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<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
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    <name>Q3SMX2_NITWN</name>
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        <submittedName>
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        </submittedName>
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        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
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            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
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        </source>
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    <evidence type="ECO:0000313" key="2">
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        </source>
    </evidence>
    <sequence length="284" mass="31481" checksum="D452874DEB452EF3" modified="2005-10-11" version="1">MPMAKKHIEMAIAYDFDGTLADGNMQEHQFLPDIGMKPAAFWAEVKRLTKEHQADEVLVYMNLMLRKAAAANVPVRRDDFKARGKAIQLFEGVEDWFDRITGYGKAQGVRVEHYLVSSGNAEIFAGTPIVSKFAQVYASKFMFDQNGVAAWPALAVNYTTKTQYLFRINKGAFDLSDNSKVNQFVEKRDRPVPFENIVFIGDGSTDIPCFRLVKEQGGLSVAVFKPHTKGARGKADSYIKDDRVHCVAPAIYTDGSELDRIIKANIAAVAARSALSGLFSEGTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMX4</accession>
    <name>Q3SMX4_NITWN</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
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        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
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        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
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    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06367.1"/>
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    </dbReference>
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        <property type="protein sequence ID" value="ABA06367"/>
        <property type="gene ID" value="Nwi_3120"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3120"/>
    <dbReference type="eggNOG" id="COG0732">
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    <dbReference type="HOGENOM" id="CLU_1188947_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
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    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Restrct_endonuc_typeI_TRD_sf"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
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    <keyword evidence="1" id="KW-0680">Restriction system</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="233" mass="26388" checksum="593A1BE1147C1BF8" modified="2005-10-11" version="1">MLKGTFQAELVKQPRVATSRSLPLTPLNPGPVPKDWQVKTIAREWKLRCLGEITRELTWRHNDRNFGRELVMGVTNSRGIVPMQTIGSDLTRYKILLPRAFAYNPMRIKVGSIARLRLPSEVLVSPDYVLFECVPGKLDPDFLNHLRQSHFWDHYINAGGSGSVRMRAYYDDLAALRLKLPGFAEQHRISAMLNTAQGEIALVATEIETLTRQTRGLMQKLPTGERRVKVGAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN30</accession>
    <name>Q3SN30_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_3063</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316227.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3063"/>
    <dbReference type="EnsemblBacteria" id="ABA06311">
        <property type="protein sequence ID" value="ABA06311"/>
        <property type="gene ID" value="Nwi_3063"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3063"/>
    <dbReference type="eggNOG" id="COG2211">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029352_1_1_5"/>
    <dbReference type="OMA" id="ANPFYVD"/>
    <dbReference type="OrthoDB" id="1165704at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004752">
        <property type="entry name" value="AmpG_permease/AT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12778">
        <property type="entry name" value="PTHR12778"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="122"/>
        </location>
    </feature>
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            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="264"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="355"/>
            <end position="381"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="420"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA06311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="452" mass="47847" checksum="38E9C4FDE363C1B2" modified="2005-10-11" version="1">MTAPDAAAPIVSDVAPRASWRESMAVYLQPRVLIVMMLGFSSGLPLALSGSTLQIWMTESGIDLRAIGLFVLVGTPYTLKFLWAPLVDALHIPLLTRRFGRRRGWLVFSQLLLIAAILLLASTDPAKAPWFVALGALLVAATSATQDIVVDAFRVESLPESEQAAGMAAYVAAYRIGMLVSTAGALFFVSALEGTGLVRASAWAWGYVGMATMVLIGTVTALIATEPEQSVRAELATRAKGGFARIVNAAVGAFSEFIARKDALAILVFIVLFKFTDAFSGSMTAPFVIDLGFTRNDYAAIVKGVGLAATLIGGFAGGFVARRYPLAASLWIGGILQAVANLSFSWLAMVGVDQWALALAITAENFTSAIGTVIFVAYLSALCRNPLHTATQYALLTALAAVGRTYLSASAGFVAEATGWPLFFVICTLVALPSLILLAWLQRRGHFAALQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN55</accession>
    <name>Q3SN55_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Peptidase C14, caspase catalytic subunit p20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_3036</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3036"/>
    <dbReference type="EnsemblBacteria" id="ABA06286">
        <property type="protein sequence ID" value="ABA06286"/>
        <property type="gene ID" value="Nwi_3036"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3036"/>
    <dbReference type="eggNOG" id="COG4249">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_968645_0_0_5"/>
    <dbReference type="OMA" id="VIGNAHY"/>
    <dbReference type="OrthoDB" id="1041250at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029030">
        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001309">
        <property type="entry name" value="Pept_C14_p20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="SSF52129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50208">
        <property type="entry name" value="CASPASE_P20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CASPASE_P20" evidence="2">
        <location>
            <begin position="57"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="295" mass="32110" checksum="1E1A883A8AC7BFF0" modified="2005-10-11" version="1">MTLGQFRVSHRSVMLAAVLVGMVSLAIGAHAALNKRSLEVAKALEAEDTTGSIAVKRARVALVIGNGHYPDANQPLRQSINDARAFAAVLRRDGFDVIMVEDADKEDMHRAVRRLQAKIGPDTTALLFFSGFGIQSGRESYMIPVDARIWRESDVRRDGLSIETVLRAMKEHAAKVKLAVIDASRRNPYERRFRSYSHGLAPINVPDNALILSSATPGRVVDESGGQYSPLMVELLKSFGQPDRDAETVFSRTRAAVSRASEGKQVPTVSSSLVQDVPFKPDTAAASRKDTAAID</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNA3</accession>
    <name>Q3SNA3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oxidoreductase, molybdopterin binding protein</fullName>
            <ecNumber evidence="7">1.7.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2988</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mo-molybdopterin</name>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="1.7.1.1" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316160.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2988"/>
    <dbReference type="EnsemblBacteria" id="ABA06238">
        <property type="protein sequence ID" value="ABA06238"/>
        <property type="gene ID" value="Nwi_2988"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2988"/>
    <dbReference type="eggNOG" id="COG2041">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003827_5_5_5"/>
    <dbReference type="OMA" id="ERHHAGI"/>
    <dbReference type="OrthoDB" id="1345710at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009703">
        <property type="term" value="F:nitrate reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005066">
        <property type="entry name" value="MoCF_OxRdtse_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008335">
        <property type="entry name" value="Mopterin_OxRdtase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000572">
        <property type="entry name" value="OxRdtase_Mopterin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036374">
        <property type="entry name" value="OxRdtase_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030835">
        <property type="entry name" value="Sulfite_DH_SoxC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03404">
        <property type="entry name" value="Mo-co_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00174">
        <property type="entry name" value="Oxidored_molyb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00407">
        <property type="entry name" value="EUMOPTERIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56524">
        <property type="entry name" value="SSF56524"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="SSF81296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04555">
        <property type="entry name" value="sulfite_DH_soxC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="3 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229166" evidence="4">
        <location>
            <begin position="33"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="Oxidored_molyb" evidence="5">
        <location>
            <begin position="109"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Mo-co_dimer" evidence="6">
        <location>
            <begin position="293"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA06238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="425" mass="46584" checksum="B168021A3278F946" modified="2005-10-11" version="1">MFPRRTTSSRRGFLFAAGSLVGAAGAATIAHAEVLKAPTGAVLYDVPDDPTKVQGRPVVDGSYGTRSQFETEARMRYPTPNENTSWSLTPLGRGVGNITPSGLHFERHHGGIPTIDPAKHNLLIHGMVERSMTFSMADIKRLPSVTRKHFIECSGNGLTEWNKPTMKTVQGTHGLLSTSEWTGVQFATFARETGLKEGAAWVLAEGADAAVMTRSIPLEKMLKDALIVYGQNGEAIRPEQGYPLRLLLPGYEGNTHIKWLRRLQVSDKPFMTREETAKYTDLLGSGKARIFSLDMEAKSVITFPSGEMKLPGPGFYNITGLAWTGRGRVQSVDVSLDSGRTWYPARLESPPEPMCTVRFSFPWMWDGKGAVLQSRCIDETGYVQPTLEQLIAIRGNNGSFGSIYHLNAIQSWAVSEAGDVTNVHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNB2</accession>
    <name>Q3SNB2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2979</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2979"/>
    <dbReference type="EnsemblBacteria" id="ABA06229">
        <property type="protein sequence ID" value="ABA06229"/>
        <property type="gene ID" value="Nwi_2979"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2979"/>
    <dbReference type="eggNOG" id="ENOG5032SJ7">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109378_1_1_5"/>
    <dbReference type="OMA" id="ECEGRRT"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009333">
        <property type="entry name" value="DUF992"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06186">
        <property type="entry name" value="DUF992"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229044" evidence="2">
        <location>
            <begin position="22"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="182" mass="18955" checksum="F2AEB46FED7C2C7D" modified="2005-10-11" version="1">MNFPKLLAAAAVTLVASVAGAQAQYQHHGVQAGVLECEGGQHVGYIVGSTTELQCIFRSSTGAPPEPYIARIQRFGLDLGVTQQTGLAWMVHAPGGRLGRGALAGHFGGVGANATVGVGIGANLLVGGSGNSISLQPLSLQGQTGFSAAAGVVDVDLRPADVRGAPRYKRHRHHHRRHRHHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SND5</accession>
    <name>Q3SND5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2956</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316128.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2956"/>
    <dbReference type="EnsemblBacteria" id="ABA06206">
        <property type="protein sequence ID" value="ABA06206"/>
        <property type="gene ID" value="Nwi_2956"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2956"/>
    <dbReference type="eggNOG" id="COG2845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_497705_0_0_5"/>
    <dbReference type="OMA" id="MADWLAY"/>
    <dbReference type="OrthoDB" id="1247435at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="377"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="460"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="491"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="538" mass="57380" checksum="ABE6D786A8D6ED98" modified="2005-10-11" version="1">MSGPRSLLRRLTQRGPWIALAIVIATLVAAPASAQLFDFGFDRPARRGGGGGGGGWFGSDNPFFAPFQQPPQRQPQQQQQRPAENYSHAPPPEKRDTVSERNVLVLGDAMADWLAYGLEDALSETSDMGVIRKHKTVSGLIRYQPKGEPADWAAAAKAILATENPDAIVVMLGLQDRMSIREAPEKEKSGKPDDRKADVDKKEGSNTGKADEAAADSDGKPADSEQSPDETADQPAASRTKSARSPNGVAEFRSDRWVELYSRKVDNMITVLKSKGVPVFWVALPAVRGTKSTADMLFLNSLYRDAADKAGITYVDIWDGFVDEAGRYIQQGPDFEGQIRRLRSYDGVFFTKAGSRKLAHYVEREITRVMAARSGPIAIPVEPGTPDTDVKPGQPAPRPLAGPIVPLVASSFGSDRLLGGPGSHPAAIDALAARTLVRGEPLSAPAGRADDFAWPRREVGLEPAKGDPPVAAAPEGAGAASAAKPNRPRPAPSAQQAQSNGPSGSRVFSPAQAGRSQASPRPPADFGLPGFFSDLFSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNI1</accession>
    <name>Q3SNI1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RND efflux system, outer membrane lipoprotein, NodT</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2910</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the outer membrane factor (OMF) (TC 1.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2910"/>
    <dbReference type="EnsemblBacteria" id="ABA06160">
        <property type="protein sequence ID" value="ABA06160"/>
        <property type="gene ID" value="Nwi_2910"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2910"/>
    <dbReference type="eggNOG" id="COG1538">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012817_13_0_5"/>
    <dbReference type="OMA" id="MYPQITL"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003423">
        <property type="entry name" value="OMP_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010131">
        <property type="entry name" value="RND_efflux_OM_lipoprot_NodT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02321">
        <property type="entry name" value="OEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01845">
        <property type="entry name" value="outer_NodT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0564">Palmitate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1134">Transmembrane beta strand</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="522" mass="55777" checksum="C7B3D2495AF117E2" modified="2005-10-11" version="1">MRSSRQNGSGFPSPGTAGKLAFSCTRCFIVAFLAAASPVLSGCAVGPDFKPPAPPDTVKYLSDRAGDRVPGHSIVRAGEVPPQWWELFRSPALNRLVEDGIAHNADLAAAEAAVRVAQANALAQRGALFPSVMGNFESSRQQVPVDLQSPLESNAHLLSLHTGQVTVSYLADVWGGTRRLVESANSQARMRAFQREGVYLTLASNIALAAIEEGRLRGHIAATRRVLGLQGEVLRLLRRQQEQGQIAQTDVAAQETAVAQTRLLLPDLEKKLAQQRHLLSFLTGRLASDDPKERFDLGSFRMPRRLPLTLPADLVRQRPDIQAAEADFQSDNAMIGIAIANRLPQITLTASGGAAAAQFTQLANPAAGLGIIAGNAAQTIFDAGRLENRQRAAEAEAEQSLAQYRSVVLAAFQNVADVLRALQAGTRAISAATGAERSATESITLVRKQVDQGQISVPLLLAAQEAYLETSLARLDAQAAQLANTAALFQALGGGWWNRDDRKQAAIKVVPEVNEAGLVLRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNI7</accession>
    <name>Q3SNI7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Possible signal peptide protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2904</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316084.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2904"/>
    <dbReference type="EnsemblBacteria" id="ABA06154">
        <property type="protein sequence ID" value="ABA06154"/>
        <property type="gene ID" value="Nwi_2904"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2904"/>
    <dbReference type="eggNOG" id="COG0526">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110678_0_0_5"/>
    <dbReference type="OMA" id="MPRTVWI"/>
    <dbReference type="OrthoDB" id="1617952at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015036">
        <property type="term" value="F:disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013740">
        <property type="entry name" value="Redoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08534">
        <property type="entry name" value="Redoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="4">
        <location>
            <begin position="11"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA06154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="175" mass="19509" checksum="289FA496F1CF6433" modified="2005-10-11" version="1">MKHPLLLFAFVLAGAVLPQRLAAHDELRAFQRGSWQTILHAHAGRPMMVNFWGVTCGPCKVELPLLGKFVKDNPGVDVVTVNADLVPNAREAALSMLRKAGLSSTENWTFSDSFVERLRYEIDPAWQGDIPRTMLIARDGKITTIEGIVELAKVREWFASQAVEADSESTRRTHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNK7</accession>
    <name>Q3SNK7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2884</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2884"/>
    <dbReference type="EnsemblBacteria" id="ABA06134">
        <property type="protein sequence ID" value="ABA06134"/>
        <property type="gene ID" value="Nwi_2884"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2884"/>
    <dbReference type="eggNOG" id="ENOG502ZSUN">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1353490_0_0_5"/>
    <dbReference type="OrthoDB" id="1777584at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229060" evidence="1">
        <location>
            <begin position="26"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="202" mass="22188" checksum="2D70BE05ECDEBF97" modified="2005-10-11" version="1">MQARMNKQTLLILTAISVAAADVHAASDLIDDAAGSQACFTRHYDAAHLAKKPNQKTTSMRFALRRETYPDSVDTPLLTFARLEIMRRNDTRPWRAIGQCAFDQQANRADGRAIVSNYAHADGIACHMTGENLDAEGGIFMIEPVDNKLKIYIDDDITMRTEARVTEGPGEYVAFGPADSIFILDRAADDACADLHKSIAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNU2</accession>
    <name>Q3SNU2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Copper/Zinc superoxide dismutase</fullName>
            <ecNumber evidence="5">1.15.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2796</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.15.1.1" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315994.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2796"/>
    <dbReference type="EnsemblBacteria" id="ABA06049">
        <property type="protein sequence ID" value="ABA06049"/>
        <property type="gene ID" value="Nwi_2796"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2796"/>
    <dbReference type="eggNOG" id="COG2032">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056632_8_1_5"/>
    <dbReference type="OMA" id="AQRGFHI"/>
    <dbReference type="OrthoDB" id="2015673at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00305">
        <property type="entry name" value="Cu-Zn_Superoxide_Dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024134">
        <property type="entry name" value="SOD_Cu/Zn_/chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001424">
        <property type="entry name" value="SOD_Cu_Zn_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10003">
        <property type="entry name" value="PTHR10003"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00080">
        <property type="entry name" value="Sod_Cu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00068">
        <property type="entry name" value="CUZNDISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49329">
        <property type="entry name" value="SSF49329"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0186">Copper</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229120" evidence="3">
        <location>
            <begin position="22"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Sod_Cu" evidence="4">
        <location>
            <begin position="38"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA06049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="169" mass="17373" checksum="C0D70969EEF3B06A" modified="2005-10-11" version="1">MIARVLFATTALLALSVTAVSANSARALLKNAEGAEVGKVELIQVKDGVLLKAALKDIPPGEHAFHIHAVGKCEPPFKSAGGHFNPDNKKHGMMAADGSHAGDLPNIVVPASGELKLDVVSTAITLDKDKPNSVLKPDGTAIVIHAAADDYKTDPTGEAGGRIACGVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNU9</accession>
    <name>Q3SNU9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2789</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315987.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2789"/>
    <dbReference type="EnsemblBacteria" id="ABA06042">
        <property type="protein sequence ID" value="ABA06042"/>
        <property type="gene ID" value="Nwi_2789"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2789"/>
    <dbReference type="eggNOG" id="ENOG502ZYRA">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_173327_0_0_5"/>
    <dbReference type="OMA" id="VIVPRMN"/>
    <dbReference type="OrthoDB" id="2015473at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="112" mass="12494" checksum="05449C0D4F7D0664" modified="2005-10-11" version="1">MDVRTAAGRLVTAAASLLLLVVPGTLLLMYGGQAQARTAARNMDRAVSSGMSAQTHKRRPRVRIYREDERGVYPSYHPGSDAVRDCTAHYVQEFRPSGTVIVPRMNCYWRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP39</accession>
    <name>Q3SP39_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2699</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315898.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2699"/>
    <dbReference type="EnsemblBacteria" id="ABA05952">
        <property type="protein sequence ID" value="ABA05952"/>
        <property type="gene ID" value="Nwi_2699"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2699"/>
    <dbReference type="eggNOG" id="ENOG5032HB8">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124286_1_0_5"/>
    <dbReference type="OMA" id="IPDMPEC"/>
    <dbReference type="OrthoDB" id="1880753at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="170" mass="18817" checksum="0E4A24CCA1FE4972" modified="2005-10-11" version="1">MTPRADESLPSPPTASRPGQNRPDSAAALAAANINPQSCLATDYLNHFNEAIMLLEMIPDMPDCVEDFLNWRPMTYQEHFMASGFKAREQAIEAYENAAPEIRAEFDRLTITMTSILTAVAAATKDIHRHETRAALATEAVSWIKPMITRAGGIINGQGRETDVDYIMNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP68</accession>
    <name>Q3SP68_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter, ATPase subunit</fullName>
            <ecNumber evidence="7">3.6.3.25</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2670</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in beta-(1--&gt;2)glucan export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.25" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2670"/>
    <dbReference type="EnsemblBacteria" id="ABA05923">
        <property type="protein sequence ID" value="ABA05923"/>
        <property type="gene ID" value="Nwi_2670"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2670"/>
    <dbReference type="eggNOG" id="COG1136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_5"/>
    <dbReference type="OMA" id="RPDSGAY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="21"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="57"/>
            <end position="64"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="236" mass="25247" checksum="087651D41F85C30C" modified="2005-10-11" version="1">MVDISSTTDGAARPIPQAPMLAIQNLTKTYCSAQEKIAVLRGVCVHVVAGESVALTGESGSGKSTLLHLIAGLDEADGGSIRIDDAEVTALSDRDRAAIRRDRIGLVFQQFNLIPSLTIADNLAFQSRIAGRHDGHWQNELVDRLGLGTLLKRYPEQLSGGQQQRVAIGRALAVRPSLFLADEPTGNLDEATADDVTDIALDLVKRTGCAFLMATHSTRLAAKLDRKVRLSAGRIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPA7</accession>
    <name>Q3SPA7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">TonB-dependent receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_2631</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Cell outer membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TonB-dependent receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2631"/>
    <dbReference type="EnsemblBacteria" id="ABA05884">
        <property type="protein sequence ID" value="ABA05884"/>
        <property type="gene ID" value="Nwi_2631"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2631"/>
    <dbReference type="eggNOG" id="COG4206">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008287_10_1_5"/>
    <dbReference type="OMA" id="NHREEWS"/>
    <dbReference type="OrthoDB" id="439303at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039426">
        <property type="entry name" value="BtuB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012910">
        <property type="entry name" value="Plug_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037066">
        <property type="entry name" value="Plug_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000531">
        <property type="entry name" value="TonB-dep_rcpt_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036942">
        <property type="entry name" value="TonB_rcpt_b-brl_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30069">
        <property type="entry name" value="PTHR30069"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07715">
        <property type="entry name" value="Plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00593">
        <property type="entry name" value="TonB_dep_Rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0798">TonB box</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229199" evidence="8">
        <location>
            <begin position="24"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="domain" description="Plug" evidence="10">
        <location>
            <begin position="100"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="TonB_dep_Rec" evidence="9">
        <location>
            <begin position="286"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="47"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="610"/>
            <end position="639"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA05884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="799" mass="85944" checksum="D1637162677EE878" modified="2005-10-11" version="1">MPFSSSRFLAMARLGAMSLPALAMGIPDDVSAQTALPGIVVNAPSPIVRHARPAPTTPARTARRGTTRPSAPQPPEIASAPQPASSDLRGTLPIVTDQFATVTVVTNEEIRRNGAATLGDLLNDKPGITGSSYAPGASSRPIIRGLDVNRVGIVENGIGSNGASDLGEDHFVPIDPLSTNQVEVIRGPAALRYGSTAIGGVVSASNNRIPEQMPCGPTPWQAYGFGVKPPVTANGCLNAETRSALSSVNNGLEGAVLIDAGADNVAVHADAYGRRADDYAIPGGRYQANTSNRSMGASVGASYLFDGGFIGASIAHNNVLYHIPGDHGADFKERIDGRQNRVTVKGEYRPDSALIDAVRIWLGAVDYRHHERGLEDAHDLSSDGIHQTFTDRSQEGRLEVQLAPYMLGAIPVTTAIGIQGGHQDLTASSPENAGALYNGLFAPNSNSRIGTYIFNEFQLAPTTRAQIAGRVEHVWLNGTMPDFPASFMPDGSAQLPIARDLSFTPASASVGLLQDLPGDLVASVTGQFVQRAPKPAELFSRGIHHATTTFDIGNPNLKIETAQSVEVGLRRAKGPLRFEATGYYTRFNNFIYRRLTGAFCDDDFASCHTHDHHDHDDHDDHDHDDHDDHGHDDAHGHGHHLNQAVYSQRDATFRGGELQFQYDAIPLWNGMFGIEGQYDIVRATFTDGTNVPRIPPQRLGGGFFYRDANWLARINLLHAFAQNNVSDVETPTPGYNRLRAEISYTTMLPGNHWLGGQAVTVGLVGDNLLNEQIRNAAAYNKDQVLLPGLNVRLFANVKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="130" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPC2</accession>
    <name>Q3SPC2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytochrome b</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">Nwi_2616</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="15">Binds 2 heme groups non-covalently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">The main subunits of complex b-c1 are: cytochrome b, cytochrome c1 and the Rieske protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the cytochrome b family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315819.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2616"/>
    <dbReference type="EnsemblBacteria" id="ABA05869">
        <property type="protein sequence ID" value="ABA05869"/>
        <property type="gene ID" value="Nwi_2616"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2616"/>
    <dbReference type="eggNOG" id="COG1290">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2857">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021957_1_0_5"/>
    <dbReference type="OMA" id="LPWLDSA"/>
    <dbReference type="OrthoDB" id="70809at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070469">
        <property type="term" value="C:respirasome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00290">
        <property type="entry name" value="cytochrome_b_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00284">
        <property type="entry name" value="Cytochrome_b_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.810.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005798">
        <property type="entry name" value="Cyt_b/b6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036150">
        <property type="entry name" value="Cyt_b/b6_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005797">
        <property type="entry name" value="Cyt_b/b6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002326">
        <property type="entry name" value="Cyt_c1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021157">
        <property type="entry name" value="Cyt_c1_TM_anchor_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027387">
        <property type="entry name" value="Cytb/b6-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016174">
        <property type="entry name" value="Di-haem_cyt_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00032">
        <property type="entry name" value="Cytochrom_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02167">
        <property type="entry name" value="Cytochrom_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00033">
        <property type="entry name" value="Cytochrome_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00603">
        <property type="entry name" value="CYTOCHROMEC1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81342">
        <property type="entry name" value="SSF81342"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81496">
        <property type="entry name" value="SSF81496"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81648">
        <property type="entry name" value="SSF81648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51003">
        <property type="entry name" value="CYTB_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51002">
        <property type="entry name" value="CYTB_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 14 15" id="KW-0349">Heme</keyword>
    <keyword evidence="12 14" id="KW-0408">Iron</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6 15 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="246"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="305"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="336"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="363"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="660"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="domain" description="CYTB_NTER" evidence="17">
        <location>
            <begin position="13"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="CYTB_CTER" evidence="18">
        <location>
            <begin position="227"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="19">
        <location>
            <begin position="458"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ABA05869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="687" mass="76214" checksum="C808BE4CED19C45F" modified="2005-10-11" version="1">MSGPSTFQPKNPVLKWIERRLPIFGLVHSSFVAYPTPRNLNYWWTFGGILSFMLGVQIVTGVILAMHYTPHADLAFNSVEAIVRDVDYGWLLRYLHSNGASMFFIAVYIHMLRGLYYGSYKEPREILWILGVIIYLLMMATGFMGYVLPWGQMSFWGATVITNLFSAIPYFGESIVTLLWGGYSVGNPTLNRFFSLHYLLPFVIAGVVVLHVWALHVAGQNNPAGVEPKSSKDTVPFTPYATIKDGFGLACFILFYFWFVFYIPNYLGDPDNYIPANPGVTPPHIVPEWYYLPFYAILRSIPDKLAGVVAMFSAILVLAFLPWLDSAKTRSMRYRPLAKQCFWIFVGVCLGLGYLGAKPPEGVYVIAGRILTACYFAYFLIVLPILSRIEKPRAVPNSIADDVLGKKKSVVASALAVIGVAGLLALDSGSTARASDHGSTPPSMSWSFAGPFGKFDQGQLQRGYKVYKEVCAACHSMNLVHYRNLTDPGGPGLSIAQAEALAAEIQVKDGPNDAGDMFERPGRIADKFPSPFPNENAARAANGGAAPPDLSLMAKARGYERGFPQFIFDAFTQFQEKGPNYIHALLTGFEDTPPEGFKLPDGSTYNRYFPGHAMKMPNVLSDDQVTYDDGAPQNVDQYAKDVAAFLMWAAEPKLEERKRLGFQVMIFMVILAGLLYFTKRAVWADAH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-16" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPE0</accession>
    <name>RNH2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease HII</fullName>
            <shortName evidence="1">RNase HII</shortName>
            <ecNumber evidence="1">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnhB</name>
        <name type="ordered locus">Nwi_2598</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNase HII family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315801.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SPE0"/>
    <dbReference type="SMR" id="Q3SPE0"/>
    <dbReference type="STRING" id="323098.Nwi_2598"/>
    <dbReference type="EnsemblBacteria" id="ABA05851">
        <property type="protein sequence ID" value="ABA05851"/>
        <property type="gene ID" value="Nwi_2598"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2598"/>
    <dbReference type="eggNOG" id="COG0164">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036532_2_2_5"/>
    <dbReference type="OMA" id="YPTKLHL"/>
    <dbReference type="OrthoDB" id="1685277at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07182">
        <property type="entry name" value="RNase_HII_bacteria_HII_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00052_B">
        <property type="entry name" value="RNase_HII_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022898">
        <property type="entry name" value="RNase_HII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001352">
        <property type="entry name" value="RNase_HII/HIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024567">
        <property type="entry name" value="RNase_HII/HIII_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954">
        <property type="entry name" value="PTHR10954"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954:SF18">
        <property type="entry name" value="PTHR10954:SF18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01351">
        <property type="entry name" value="RNase_HII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51975">
        <property type="entry name" value="RNASE_H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000235740" description="Ribonuclease HII">
        <location>
            <begin position="1"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="RNase H type-2" evidence="2">
        <location>
            <begin position="48"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="258" mass="27425" checksum="9292D421109D6AB6" modified="2005-10-11" version="1">MRVAPSGGPPHHHAMIRATPPKRRLKAGTINVAPSFMRERDLMECGVWPVAGCDEVGRGPLAGPVVAAAVVLDPNHIPKGIDDSKRLTAARREELFEEIVATASFAVACAPIERIDRDNILRASLWALARAVHALPDTPKHVFVDGRDRIDIACACEPVIGGDGLVLSIAAASIVAKVTRDRLMCKLARDCPGYGFETHKGYAVPGHLEALGRLGPSSHHRSFFAPVVAARERHRALLAGASASISETSATVAVEAVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPH1</accession>
    <name>Q3SPH1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase, family 2</fullName>
            <ecNumber evidence="3">2.4.1.83</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2567</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.1.83" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315771.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2567"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05820">
        <property type="protein sequence ID" value="ABA05820"/>
        <property type="gene ID" value="Nwi_2567"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2567"/>
    <dbReference type="eggNOG" id="COG0463">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033536_0_1_5"/>
    <dbReference type="OMA" id="PLRMWTY"/>
    <dbReference type="OrthoDB" id="1064289at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004582">
        <property type="term" value="F:dolichyl-phosphate beta-D-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="252"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="285"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="23"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="348" mass="37880" checksum="DC6E15DEFED013C7" modified="2005-10-11" version="1">MTPPSSLPGLPADEATAIAQGLSIIVPVYNEAAGLEQLHERLSALAAKLRQRFSLACEVVYVDDGSTDDTLKLARALSATVLDVQVVSLSRNFGKEAALMAGLDHARRGALLFMDGDGQHSPDLVERLVDHWINDGFDVIYTAKSHRTNESLLRRIGVHSFYAIINWGARQRIPEDAGDFRLLSPRAAAALRRMPERNRFFKGLASWIGFRQFRVDYEPDVRAHGMTTFNAARLLGLSIEGLTSFSVAPLRLASLLGLLLALAAFLFGLSILWETLVDGESVPGYPSLVVGIMTLGGVQLLMIGIMGEYIGKILSELKARPIYFVAEHSVKQAESEPSAGDPPRTAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPH5</accession>
    <name>Q3SPH5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2563</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2563"/>
    <dbReference type="EnsemblBacteria" id="ABA05816">
        <property type="protein sequence ID" value="ABA05816"/>
        <property type="gene ID" value="Nwi_2563"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2563"/>
    <dbReference type="eggNOG" id="ENOG502ZIBH">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1259434_0_0_5"/>
    <dbReference type="OMA" id="WCSIPDS"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="256" mass="25950" checksum="54244923D9F1C8C2" modified="2005-10-11" version="1">MIVPRMIVPLTLAVFVIGTTGVLGQTPFPAPLPNGGTGTASDSPFPPVNGAPAQKTASPQDSPFPPVNGAPAQRAATSQASPFPPVNGASATMSAPSAFPSGGATPLGGFGAPQQQAGPPGGADCMKDFLPLRDAAEKRGKLIQAASARQAPPDEACKLIGQYAQAEARMIKFIDANSNRCGIPDNVGKQMHANHKSTESMQQKVCDVAKQAKMRGPAGPSLSEALGSASGGLPEVTTPKRGGSTFDTLSGNVLTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPI7</accession>
    <name>Q3SPI7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA-specific adenosine deaminase</fullName>
            <ecNumber evidence="3">3.5.4.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">tadA</name>
        <name evidence="5" type="ordered locus">Nwi_2551</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the deamination of adenosine to inosine at the wobble position 34 of tRNA(Arg2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>adenosine(34) in tRNA + H(+) + H2O = inosine(34) in tRNA + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:43168"/>
            <dbReference type="Rhea" id="RHEA-COMP:10373"/>
            <dbReference type="Rhea" id="RHEA-COMP:10374"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:82852"/>
            <dbReference type="EC" id="3.5.4.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.33" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315755.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2551"/>
    <dbReference type="EnsemblBacteria" id="ABA05804">
        <property type="protein sequence ID" value="ABA05804"/>
        <property type="gene ID" value="Nwi_2551"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2551"/>
    <dbReference type="eggNOG" id="COG0590">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025810_3_2_5"/>
    <dbReference type="OMA" id="PCQMCAG"/>
    <dbReference type="OrthoDB" id="1890883at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052717">
        <property type="term" value="F:tRNA-specific adenosine-34 deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002100">
        <property type="term" value="P:tRNA wobble adenosine to inosine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00972">
        <property type="entry name" value="tRNA_aden_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028883">
        <property type="entry name" value="tRNA_aden_deaminase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14437">
        <property type="entry name" value="MafB19-deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="2 3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="4">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="148" mass="15865" checksum="5915658C5CC41584" modified="2005-10-11" version="1">MTTPSFMDMALEAAEKAGQAGEVPIGCVIVRDGEIIAAAGNRTLTDRDPTAHAEVLALRAAAHVLGSERLTGCDLYVTLEPCTMCAAAISFARIRRLYYGASDPKGGAVEYGVRFFAAPTCHHRPEVYSSVGESQAATLLRDFFKARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPK0</accession>
    <name>Q3SPK0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Diguanylate phosphodiesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2538</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315742.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2538"/>
    <dbReference type="EnsemblBacteria" id="ABA05791">
        <property type="protein sequence ID" value="ABA05791"/>
        <property type="gene ID" value="Nwi_2538"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2538"/>
    <dbReference type="eggNOG" id="COG2200">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036071_0_0_5"/>
    <dbReference type="OMA" id="FAQCLDF"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="36"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="EAL" evidence="3">
        <location>
            <begin position="186"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="432"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="479" mass="50942" checksum="02C3C5B729E3E14F" modified="2005-10-11" version="1">MIRISTIFIALCMVLVSISVGIVLYSLAGLPASECAVVTLAVLTVLILYNAVSMRVRGRSTAEGQIADLSRGVADLARQVSDFGRRLAAAESKIVQANSAASNRTQSGLESALGEINELGGLVKQLAAMVAAHDDMLTALPSVASAPAIEPAPPARPADREALARAISATPAVIRTTASSARSQHDAQTLATIKNAIEANGVDLFLQPIVTLPQRKVRYYEAMTRLRNAGGAYLAADAFIPAAESADLMGKLDNTVLSRCVQVLQRLHARNKDAGLFCNIAMASLGNPETFRQCRDILEANRAFASSLILEFKYATLRGLGITESEHLAALAKLGCRFSVDHVTTLRIEPRELADRGVRFIKAPASLLLDPKQSSTSDIHPADMSDLLGRFGIDLIAERIEDEKTVVDLLDYNVRFGQGFLFAVPRPLRQEAADEPADKAKPRASGAAGTNTPGGAIKTEHSPRMTGNAALARRSGDAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPN3</accession>
    <name>Q3SPN3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2505</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315709.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2505"/>
    <dbReference type="EnsemblBacteria" id="ABA05758">
        <property type="protein sequence ID" value="ABA05758"/>
        <property type="gene ID" value="Nwi_2505"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2505"/>
    <dbReference type="eggNOG" id="COG2960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154412_1_2_5"/>
    <dbReference type="OMA" id="MQTRNKI"/>
    <dbReference type="OrthoDB" id="1995361at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007475">
        <property type="entry name" value="UbiK"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04380">
        <property type="entry name" value="BMFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="85" mass="9535" checksum="F90E0EE881294BAE" modified="2005-10-11" version="1">MTQTSNRFFDEIGRLMNDTAGVAQGFKREVDAVVRHQAEKILSDLDVVKREEFEAVKEMARLAREDNEALKARIAALEAKLGQTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ18</accession>
    <name>Q3SQ18_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2369</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315585.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2369"/>
    <dbReference type="EnsemblBacteria" id="ABA05623">
        <property type="protein sequence ID" value="ABA05623"/>
        <property type="gene ID" value="Nwi_2369"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2369"/>
    <dbReference type="eggNOG" id="COG0265">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_749714_0_0_5"/>
    <dbReference type="OMA" id="AKTINAR"/>
    <dbReference type="OrthoDB" id="932262at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229118" evidence="2">
        <location>
            <begin position="23"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="369" mass="39834" checksum="E7D637FE9E594901" modified="2005-10-11" version="1">MNRLLMICITLVMSVWWSDANARGPFGSIKIAGWSGGAYTDNKTGEFSNCIVSATYKSGITFGIVVTKKLEWLLAFTHKRWNLAPKEKFPIALSFDGRNTFNVIGVSLSTNTVFVPMPDNSAVIRSFRSAQIMSAIAQGNVFQFRLTGTSAVLPAMVTCAKTINARGLAAATDFVAPHQPAITPPSMGPQRTTATLSSTQPNSAHENSTELQFEAMQIASNFILKASLDHPSLLRREDTPLSIASTGAGWKANDATGFVRIIPAKQNMTGLDVAATIVGNDAKECKGKFASARNSELIDSEVVFRGMSSCEDSKGSVVAEYFVFPRKKGGFILFSVLTPTKTGSQQTQAQNREEMNAGFRQAAYTSLTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ20</accession>
    <name>Q3SQ20_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosomal protein L11 methyltransferase</fullName>
            <shortName evidence="2">L11 Mtase</shortName>
            <ecNumber evidence="2">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">prmA</name>
        <name evidence="3" type="ordered locus">Nwi_2367</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Methylates ribosomal protein L11.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-lysyl-[protein] + 3 S-adenosyl-L-methionine = 3 H(+) + N(6),N(6),N(6)-trimethyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:54192"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:13826"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the methyltransferase superfamily. PrmA family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2367"/>
    <dbReference type="EnsemblBacteria" id="ABA05621">
        <property type="protein sequence ID" value="ABA05621"/>
        <property type="gene ID" value="Nwi_2367"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2367"/>
    <dbReference type="eggNOG" id="COG2264">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049382_3_0_5"/>
    <dbReference type="OMA" id="EFFFIFP"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00735">
        <property type="entry name" value="Methyltr_PrmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004498">
        <property type="entry name" value="Ribosomal_PrmA_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="321" mass="34294" checksum="A96EAA69F7F81861" modified="2005-10-11" version="1">MPNISELRSSGILTLPRICSGLPMTTEISPATSRAAFAIGAEQVARRAVDFLSESLEDGETAVTAFERSDGCWSVAVHFADPPDEARVRALVALAAGDDVARTVVFDTIAARDWVEASLEGLAPVPAGRFIVHGRHDRDRVPPNKLAIEIEAALAFGTGHHGTTRGCLLLLDHVLRARMPRRVLDLGAGTGVLAIAAAKALHRSVLASDIDPRSATVARENATLNGVGSLVWSIRATGFSAPLFRARAPFDLVLANILANPLRRMAPAMARHLAPEAMVILSGLLPQHTRGVIAAYRAQGLVLIRQLRIDGWCSLLMQNRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ42</accession>
    <name>Q3SQ42_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphoserine phosphatase</fullName>
            <ecNumber evidence="6">3.1.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">O-phosphoserine phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">Nwi_2345</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H2O + O-phospho-D-serine = D-serine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24873"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35247"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58680"/>
            <dbReference type="EC" id="3.1.3.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + O-phospho-L-serine = L-serine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21208"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57524"/>
            <dbReference type="EC" id="3.1.3.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the HAD-like hydrolase superfamily. SerB family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.3" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315563.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2345"/>
    <dbReference type="EnsemblBacteria" id="ABA05599">
        <property type="protein sequence ID" value="ABA05599"/>
        <property type="gene ID" value="Nwi_2345"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2345"/>
    <dbReference type="eggNOG" id="COG0560">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036368_4_2_5"/>
    <dbReference type="OMA" id="CINEPDL"/>
    <dbReference type="OrthoDB" id="1755462at2"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00198"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036424">
        <property type="term" value="F:L-phosphoserine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004469">
        <property type="entry name" value="PSP"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00029">
        <property type="entry name" value="phosphoserine_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00338">
        <property type="entry name" value="serB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0718">Serine biosynthesis</keyword>
    <feature type="active site" description="Nucleophile" evidence="14">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="14">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00015196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR604469-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABA05599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="297" mass="31432" checksum="80D15E0FA497206E" modified="2005-10-11" version="1">MSLVATLICNPANPALDSTVLDGARAILGNAGPAQWLWDEVAADIPFESTDSIPAITQRLREARGDLPIDVVVQPQIGRRKKLFLADMDSTMIGQECIDELADFAGLKAHVAAITERAMRGEMQFEAALRERVALLKGLPVSVVDEVLAKRITPTPGGRELVMTMRANGAYTCLISGGFTLFTEAVAALIGFHENRGNTLLVENGKLSGTAAEPIVGREAKLTTLIELRESSGLDGLDTLAVGDGANDLGMIQQAGLGVAYHAKPAVAAAAAARIDHGDLTALLYAQGYQRDEFVNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ84</accession>
    <name>Q3SQ84_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fec I like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2303</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315524.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2303"/>
    <dbReference type="EnsemblBacteria" id="ABA05557">
        <property type="protein sequence ID" value="ABA05557"/>
        <property type="gene ID" value="Nwi_2303"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2303"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_12_3_5"/>
    <dbReference type="OMA" id="NPRAYLY"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="2">
        <location>
            <begin position="11"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="3">
        <location>
            <begin position="104"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="166" mass="18808" checksum="6E31F2E0E66338D1" modified="2005-10-11" version="1">MSSTTELLNIFKAEQGQLQRLVRRLTGNPHTAEDVVHDTFIKLMHRDVGKCDVGLVVTTARNLARDAMRAERVRTSYAESVSREQVHGGVVGPEDAVSSRMDLDALLRALETLPRRTQRIFLLSKVDGMTYPQIARSLGISVSTVEKEMISALEFCRNWRRRRENV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQB8</accession>
    <name>Q3SQB8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Terpene synthase/Squalene cyclase</fullName>
            <ecNumber evidence="6">5.4.99.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_2269</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis; hopanoid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the terpene cyclase/mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.17" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315490.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2269"/>
    <dbReference type="EnsemblBacteria" id="ABA05523">
        <property type="protein sequence ID" value="ABA05523"/>
        <property type="gene ID" value="Nwi_2269"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2269"/>
    <dbReference type="eggNOG" id="COG1657">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019345_0_0_5"/>
    <dbReference type="OMA" id="CWARQTI"/>
    <dbReference type="OrthoDB" id="260889at2"/>
    <dbReference type="UniPathway" id="UPA00337"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051007">
        <property type="term" value="F:squalene-hopene cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016104">
        <property type="term" value="P:triterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02892">
        <property type="entry name" value="SQCY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006400">
        <property type="entry name" value="Hopene-cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032696">
        <property type="entry name" value="SQ_cyclase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032697">
        <property type="entry name" value="SQ_cyclase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018333">
        <property type="entry name" value="Squalene_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002365">
        <property type="entry name" value="Terpene_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11764">
        <property type="entry name" value="PTHR11764"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13243">
        <property type="entry name" value="SQHop_cyclase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13249">
        <property type="entry name" value="SQHop_cyclase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01016">
        <property type="entry name" value="Prenyltransferase_Like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="SSF48239"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01507">
        <property type="entry name" value="hopene_cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01787">
        <property type="entry name" value="squalene_cyclas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01074">
        <property type="entry name" value="TERPENE_SYNTHASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SQHop_cyclase_N" evidence="5">
        <location>
            <begin position="22"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="SQHop_cyclase_C" evidence="4">
        <location>
            <begin position="319"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="654" mass="72432" checksum="4F0700502E591C74" modified="2005-10-11" version="1">MNSINATAAPIDDNVLGDRIGAATRGLLSLKQSDGHFVFELEADATIPSEYILMRHYLGEPVDTVLEAKIAAYLRRIQGAHGGWPLVHDGPFDMSASVKAYFALKMAGDSIDAPHMARAREAILSRGGAANVNVFTRFLLSFFGELTWRSVPVLPVEIMLLPMWSPFHLNKVSYWARTTMVPLMVLAALKPRARNPRGIGIRELFLEDPATVGTPKRAPHQSPGWFALFTGFDRVLRLIEPLSPKWLRARAMKKAIAFVEERLNGEDGLGAIFPPMVNTVMMYDALGFPPEHPPRAVTRRGIDKLLVVGENEAYCQPCVSPIWDTALSCHALLEAGGPEAVNSAGKCLDWLLLKQELVLKGDWAVKRPDVRPGGWAFQYANGHYPDLDDTAVVVMAMDRVRRNGPNGRYDEAIARGREWIEGMQSRDGGFAAFDADNLEYYLNNIPFSDHAALLDPPTEDVTARCVSMLAQLGETVDSSSSMAAGVEYLRRTQLAEGSWYGRWGLNYIYGTWSVLCALNVAGVDHQDPVIRRAVNWLVSIQNADGGWGEDAVSYRLDYKGFEGAPTTASQTAWALLALMAAGEVENPAVARGIKYLIDTQTKKGLWDEQRYTATGFPRVFYLRYHGYSKFFPLWALARYRNLRSTNSKAVGVGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQE2</accession>
    <name>Q3SQE2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CsbD-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2245</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0337 (CsbD) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315467.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05499">
        <property type="protein sequence ID" value="ABA05499"/>
        <property type="gene ID" value="Nwi_2245"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2245"/>
    <dbReference type="eggNOG" id="COG3237">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135567_4_1_5"/>
    <dbReference type="OMA" id="NWDRIEG"/>
    <dbReference type="OrthoDB" id="2089955at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008462">
        <property type="entry name" value="CsbD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026042">
        <property type="entry name" value="YjbJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036629">
        <property type="entry name" value="YjbJ_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05532">
        <property type="entry name" value="CsbD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039008">
        <property type="entry name" value="YjbJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69047">
        <property type="entry name" value="SSF69047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CsbD" evidence="2">
        <location>
            <begin position="4"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="67" mass="8075" checksum="35D121CE6B4FABFB" modified="2005-10-11" version="1">MDWNRVEGSWKQLKGSVKEKWGKLTDDDLDVINGKREQLEGKIQERYGYQKDQAKKEIDDWFGSRKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQJ8</accession>
    <name>Q3SQJ8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Molybdopterin-biosynthesis enzyme MoeA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2189</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315412.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2189"/>
    <dbReference type="EnsemblBacteria" id="ABA05443">
        <property type="protein sequence ID" value="ABA05443"/>
        <property type="gene ID" value="Nwi_2189"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2189"/>
    <dbReference type="eggNOG" id="COG1058">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030805_0_2_5"/>
    <dbReference type="OMA" id="IGNVIVM"/>
    <dbReference type="OrthoDB" id="1834406at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00885">
        <property type="entry name" value="cinA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="SSF53218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoCF_biosynth" evidence="1">
        <location>
            <begin position="8"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="246" mass="26566" checksum="DA1A41CB80376973" modified="2005-10-11" version="1">MGDIVTAGVLVIGDEILSGRTKDKNIGFIAEYLTNIGIDLKEVRIVADDESDIVAAVNALRARYTYVFTTGGIGPTHDDITADSVAKALDVSIDHHPEVVARFKERYQKPGELNEARLRMARIPQGAQPIQSATILAPGFRIDNVIVMAGVPSIMQAMMDIVAPTLRVGDRMLSESVRGNCREGDIGTPLREIAEAHPDTAIGSYPFLDENGKPNTSIVVRSRDQANLTTAMDAVKAMLATLKVLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQS8</accession>
    <name>Q3SQS8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase for insertion sequence element ISRM11/ISRM2011-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2108</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2108"/>
    <dbReference type="EnsemblBacteria" id="ABA05363">
        <property type="protein sequence ID" value="ABA05363"/>
        <property type="gene ID" value="Nwi_2108"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2108"/>
    <dbReference type="eggNOG" id="COG3335">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056788_10_0_5"/>
    <dbReference type="OMA" id="IRRREIC"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0814">Transposable element</keyword>
    <feature type="domain" description="DDE_3" evidence="2">
        <location>
            <begin position="44"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="227" mass="26251" checksum="6C358650BAF054D4" modified="2005-10-11" version="1">MGVRSRCEAQLQKKTLIAAEQDRPDVARRRAQWTKYRDRIDPTRLVFIDETWTKTNMAPLRGWAPRGQRIRAKVPHGRWQTMTFMAALRHDRITAPWFIEGPINGEAFLLYIEKVLVPTLRHGDIVIMDNLGSHKASAVRRVIRAAGARLFYLPKYSPDLNPIEQFFAKFKHWLRKAAQRTTEAVYNAIAPILETVAPAECANYFVNAAHSSIFSYRNALNFQHNFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQT9</accession>
    <name>Q3SQT9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Probable TrbK</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2095</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315330.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2095"/>
    <dbReference type="EnsemblBacteria" id="ABA05352">
        <property type="protein sequence ID" value="ABA05352"/>
        <property type="gene ID" value="Nwi_2095"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2095"/>
    <dbReference type="eggNOG" id="ENOG5032T5X">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159824_0_0_5"/>
    <dbReference type="OMA" id="CLRVWAE"/>
    <dbReference type="OrthoDB" id="2054047at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027587">
        <property type="entry name" value="TrbK"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20084">
        <property type="entry name" value="TrbK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04360">
        <property type="entry name" value="other_trbK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="88" mass="9253" checksum="20E87B4C5AD6AEAF" modified="2005-10-11" version="1">MDWKLAARVVAVLAVAVVMVACAIALRPDATEEAPAPTVQPQSGEPASSELLRCREIGAAALDDAGCKKAWAEGRRHFLEGNSGRARP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-11-03" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQW4</accession>
    <name>DABA2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable inorganic carbon transporter subunit DabA 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dabA2</name>
        <name type="ordered locus">Nwi_2069</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of an energy-coupled inorganic carbon pump.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a complex with DabB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the inorganic carbon transporter (TC 9.A.2) DabA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315306.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SQW4"/>
    <dbReference type="STRING" id="323098.Nwi_2069"/>
    <dbReference type="EnsemblBacteria" id="ABA05327">
        <property type="protein sequence ID" value="ABA05327"/>
        <property type="gene ID" value="Nwi_2069"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2069"/>
    <dbReference type="eggNOG" id="COG3002">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009885_0_0_5"/>
    <dbReference type="OMA" id="CASHLDE"/>
    <dbReference type="OrthoDB" id="179501at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01871">
        <property type="entry name" value="DabA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018752">
        <property type="entry name" value="DabA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38344">
        <property type="entry name" value="PTHR38344"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10070">
        <property type="entry name" value="MpsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000387280" description="Probable inorganic carbon transporter subunit DabA 2">
        <location>
            <begin position="1"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="460"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="462"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="719"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="734"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01871"/>
        </source>
    </evidence>
    <sequence length="1037" mass="116980" checksum="60AF9B8DDFA3485F" modified="2005-10-11" version="1">MVDALNLGRRLRVRSTAYVASELVPFFWPMRNFISRNPLRRLESMPFEQAVRRGAALFHARMFLPRGDYQRWRREGKVRPETLDEEIGRRSQDLPPVPGVDWPRWLHALMQTPHDRDAVVRGVRAKDVHAALRGHPPSAQAVDVAALLPELEQRLHARTLPEAVDALWGTGLADELDDLVIKSYLDFFDEDQSAWRMPGRERGLFSAWSEVTRRNARMVLRRLHVRHILDHVQDAESAVVYVMEEMGIGAEAWPTYFTRVLTRLHGWTGFVRWRASAKHYYWAQQYPADIVDLLAIRLVMGLALLQESARHRGTPMRRDDLGAVVRERGAECVLRYALHSGEVLPDWAQRIDDTLSRGNGARCHDLLQRYWPLWQARLGQQQAAALRELAAAANATAALDALAPEDVEGLLQGLRDFAPQEGMVWTLAMEGQAIDKLLTQVQAPQDPPPDKRPFAQALFCIDVRAEPIRRHLERVGNYQTFGIAGFFGVPVGFLGYGKGSESHYCPAVITPKNLVLELPAALDPHNEDFVSTLGHVLHDLKKSVLSPYVTVEAVGMLFGLDLFGKTLAPLGYSRWRRRIDTEKPVTRLLVDKLSREQADSIIRTLQRAMIVKALHTELKIERERVDSGIIRELREIALRRRDGPTRLRTTFGVPQTQETEFIDKLRQVYGVDADYTNHQLERLGRIGYSLDEQVNYVHTALTMIGLTQTFSRFVLVVGHGGKTENNPYESALDCGACGGASGIVNARVFAQMANKAAVRERLAAMGITIPEDTWFMPALHVTTTDAIELFDLDLLPPRHLVYLERLRNSLRAASRLTAAERMPKLLPEAKALEPAEALRLANRLAVDWAQVRPEWGLSGNVYGIVGRRALTENSDLQGSAFLLSYDWRCDPRGRLLENLLTGPVVVGQWINLEYFFSTVDNSRLGSGSKVYHNVSGRFGVMTGSLSDLRTGLPMQTVMREGRPYHEPMRLIALIEAPLDFAGRVLERVVKVKSLVLGGWIRAIVIDPTQGYKPFVFNNGQWEERTPLIAPAEKEYSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQY8</accession>
    <name>Q3SQY8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2044</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315286.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2044"/>
    <dbReference type="EnsemblBacteria" id="ABA05303">
        <property type="protein sequence ID" value="ABA05303"/>
        <property type="gene ID" value="Nwi_2044"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2044"/>
    <dbReference type="eggNOG" id="ENOG5030HAM">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127079_0_0_5"/>
    <dbReference type="OMA" id="LGFMPKY"/>
    <dbReference type="OrthoDB" id="1860420at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016922">
        <property type="entry name" value="UCP029505"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029505">
        <property type="entry name" value="UCP029505"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="176" mass="19547" checksum="43BE52E9D068431E" modified="2005-10-11" version="1">MTALASPSRGISLWQRWRFHLSALVLIVPILALPTFFDNNAFFNGKKGLGQREIGEIAVGPWSVRFAEFKLSEPDAEGRAGHAKEFALALCPACVDQVKAAYLRVGKPRNIRTAGALFSGTPYRQTAEMRVPERTTADDELWLTLEGWDGTLHQATIPIRTASPVMAAWLEKRGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR54</accession>
    <name>Q3SR54_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Plasmid segregation oscillating ATPase ParF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1978</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315224.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1978"/>
    <dbReference type="EnsemblBacteria" id="ABA05237">
        <property type="protein sequence ID" value="ABA05237"/>
        <property type="gene ID" value="Nwi_1978"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1978"/>
    <dbReference type="eggNOG" id="COG1192">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037612_5_3_5"/>
    <dbReference type="OMA" id="SPMDLWA"/>
    <dbReference type="OrthoDB" id="1631847at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CbiA" evidence="1">
        <location>
            <begin position="7"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="211" mass="22303" checksum="B9917A7883581A1E" modified="2005-10-11" version="1">MSGPLVIAVINQKGGAGKTTVAMHLGAGLARRASAVVVDLDPQGSATQWAAAGTQTFPATVKHLRVGAGAVDLRRDFRAYPFVVLDCPPAVDSRAAALALQSCDVAVIPVLPSPMDLWASLRLPQDIDEARRNNPRLRAYLLPNQIETRSALSAAMQEALAEFGLPVLRTAVRRRGIYKTAALEGVSAYQLGARGQSAVAEIEGLIEEILA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRL5</accession>
    <name>Q3SRL5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1815</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315072.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1815"/>
    <dbReference type="EnsemblBacteria" id="ABA05076">
        <property type="protein sequence ID" value="ABA05076"/>
        <property type="gene ID" value="Nwi_1815"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1815"/>
    <dbReference type="HOGENOM" id="CLU_2131602_0_0_5"/>
    <dbReference type="OMA" id="GPSHISK"/>
    <dbReference type="OrthoDB" id="2190016at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="114" mass="12140" checksum="671BE7754703FC63" modified="2005-10-11" version="1">MTTPAMTIGKSLAVAILAASPCIGVTGSARAELPPRFTVWQDFAAIVAMRDFPERLGVVDRIERIGMGRFNIYGGNCAILATVVREPAKSPGGRPMPGPSRIVRVDLGEKQCKS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRN4</accession>
    <name>QUEA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">S-adenosylmethionine:tRNA ribosyltransferase-isomerase</fullName>
            <ecNumber evidence="1">2.4.99.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Queuosine biosynthesis protein QueA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">queA</name>
        <name type="ordered locus">Nwi_1796</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7-aminomethyl-7-carbaguanosine(34) in tRNA + S-adenosyl-L-methionine = adenine + epoxyqueuosine(34) in tRNA + H(+) + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:32155"/>
            <dbReference type="Rhea" id="RHEA-COMP:10342"/>
            <dbReference type="Rhea" id="RHEA-COMP:10346"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:82833"/>
            <dbReference type="ChEBI" id="CHEBI:82834"/>
            <dbReference type="EC" id="2.4.99.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; tRNA-queuosine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the QueA family.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.17" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315053.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRN4"/>
    <dbReference type="SMR" id="Q3SRN4"/>
    <dbReference type="STRING" id="323098.Nwi_1796"/>
    <dbReference type="EnsemblBacteria" id="ABA05057">
        <property type="protein sequence ID" value="ABA05057"/>
        <property type="gene ID" value="Nwi_1796"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1796"/>
    <dbReference type="eggNOG" id="COG0809">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039110_1_1_5"/>
    <dbReference type="OMA" id="YSYGDGM"/>
    <dbReference type="OrthoDB" id="368001at2"/>
    <dbReference type="UniPathway" id="UPA00392"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051075">
        <property type="term" value="F:S-adenosylmethionine:tRNA ribosyltransferase-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008616">
        <property type="term" value="P:queuosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1780.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00113">
        <property type="entry name" value="QueA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003699">
        <property type="entry name" value="QueA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042118">
        <property type="entry name" value="QueA_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042119">
        <property type="entry name" value="QueA_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036100">
        <property type="entry name" value="QueA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30307">
        <property type="entry name" value="PTHR30307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02547">
        <property type="entry name" value="Queuosine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111337">
        <property type="entry name" value="SSF111337"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00113">
        <property type="entry name" value="queA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0671">Queuosine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000231351" description="S-adenosylmethionine:tRNA ribosyltransferase-isomerase">
        <location>
            <begin position="1"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00113"/>
        </source>
    </evidence>
    <sequence length="360" mass="39098" checksum="EAEB0182478678A4" modified="2005-10-11" version="1">MRTDLFDFELPAGSIALRPASPRDSARMLVVQPGSGVRDQQVSDLPEWLRPGDQLVVNDTRVIAAQLHGRRIGREAEPRIDATLIKRLDGSRWSALVRPARKLMAGDIVRLGNEGRVCFLGHLDARVESKGEEGEVTFAFSFHGPMLDQAIAELGSTPLPPYIASKRAPDDRDASDYQTMFATHEGAVAAPTAGLHFTPEIETALRGRGVGLHRITLHVGAGTFLPVKVDDTAGHRMHSEWGAISTETADALNAARSRGGRIVAVGTTSLRLLESAADDDGRIKPFTGETAIFITPGHRFRAVDILMTNFHLPRSTLFMLVSAFSGLETMKQAYAHAISAGYRFYSYGDACLLFPERAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRR5</accession>
    <name>Q3SRR5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Serine/threonine protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1765</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315022.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1765"/>
    <dbReference type="EnsemblBacteria" id="ABA05026">
        <property type="protein sequence ID" value="ABA05026"/>
        <property type="gene ID" value="Nwi_1765"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1765"/>
    <dbReference type="eggNOG" id="COG0318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2112">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_773100_0_0_5"/>
    <dbReference type="OMA" id="AKEQNWL"/>
    <dbReference type="OrthoDB" id="607446at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="366" mass="41375" checksum="7221FABC7FE97857" modified="2005-10-11" version="1">MPLPKQDAAFLSTNWTEGVLLKRDVFSTVERGRFRTTSGEADAVLRRLDQVPWWSYVVARHLFEREREALTLARDLGVGPRLLWSGRSALVRDFIDGVALHLARPHGDPSYFRSAKAALRKLHRAGICHNDLAKEQNWLRGSDGNAYLTDFQLAACFKRRSRLFRIAAYEDLRHLLKHKRTYAPNALTPRERKMLARKSVIARVWLMTGKKVYQVITRGLFSFTDREGGGRRLVNDAPVLVARIRENPAVRDAAIVAFADRRSGVGLYAFVEADLPGLEARLRSTLAAAGEPKPPEHIQIVARLPRGAGGKVRTEILQLIAMNQIDLIEPLITSPADREFLRGILESRKNLRDRFNFESTDQALPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRY0</accession>
    <name>Q3SRY0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Heat shock protein DnaJ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1700</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1700"/>
    <dbReference type="EnsemblBacteria" id="ABA04961">
        <property type="protein sequence ID" value="ABA04961"/>
        <property type="gene ID" value="Nwi_1700"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1700"/>
    <dbReference type="eggNOG" id="COG2214">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017633_13_1_5"/>
    <dbReference type="OMA" id="WWLGRRN"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="SSF46565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0346">Stress response</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="244"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="298" mass="31934" checksum="7CB41BD772F74F9F" modified="2005-10-11" version="1">MSSGLTGSVLTGDAIPACVRKRFQNNGSGLASSWRRYVAAGHRIQSLSSTAPSYYLFAMPTLIAGLFTFLFLYLLLQLLRAANPAVLARAIRLAGGIIAFGMAAFIGSRGELAVALPLALLGVGLLGWSSSGMSFFARLFGAGMQRTAHGHTSQVRSRFLEMVLDHASGRLGGWIIDGPDAGRSLDDFDLPRLLAMMTGFDAESCALLEAYLDRRFPAWRHNAQGDPAGGRSRASPGGKMTDEEAYQILGLQPGAGNDEIRRAHRGLMKKLHPDQGGSTYLAARVNAAKDTLLRTHRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRZ0</accession>
    <name>Q3SRZ0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Malonyl CoA-acyl carrier protein transacylase</fullName>
            <ecNumber evidence="2 5">2.3.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_1690</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>holo-[ACP] + malonyl-CoA = CoA + malonyl-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:41792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="EC" id="2.3.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the fabD family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.39" evidence="2 5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314949.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1690"/>
    <dbReference type="EnsemblBacteria" id="ABA04951">
        <property type="protein sequence ID" value="ABA04951"/>
        <property type="gene ID" value="Nwi_1690"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1690"/>
    <dbReference type="eggNOG" id="COG0331">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030558_0_1_5"/>
    <dbReference type="OMA" id="AANYNCP"/>
    <dbReference type="OrthoDB" id="1123553at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004314">
        <property type="term" value="F:[acyl-carrier-protein] S-malonyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024925">
        <property type="entry name" value="Malonyl_CoA-ACP_transAc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004410">
        <property type="entry name" value="Malonyl_CoA-ACP_transAc_FabD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000446">
        <property type="entry name" value="Mct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="SSF52151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="SSF55048"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00128">
        <property type="entry name" value="fabD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PKS_AT" evidence="7">
        <location>
            <begin position="6"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR000446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000446-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA04951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="317" mass="32139" checksum="59ABF7847EFC0F7B" modified="2005-10-11" version="1">MTAAFTFPGQGSQTVGMGKALADAFPVAKAVFAEVDAALGEKLTAIIWDGPAETLQLTENTQPALMAVSIAALRVLEAEAGLSVARDAAFVAGHSLGEYSALAAAGSLSIADTARLLRTRGLAMQKAVPVGDGAMAALLGLDYEVAVAVADEAAQGQVCEAANDNGGGQIVVSGDKAAVDRAVEIAKAKGARRAMLLAVSAPFHCRLMQPAADTMAEALARVTINKPVAPVVANVIAAPVSDPDAIRKLLVKQVTGTVRWRESVAYMAAQGVTHFLEVGAGKVLTGLLKRIVKDAAGVAVGGPGDIAAARDAILAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRZ5</accession>
    <name>Q3SRZ5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Endolytic murein transglycosylase</fullName>
            <ecNumber evidence="7">4.2.2.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Peptidoglycan polymerization terminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">mltG</name>
        <name evidence="9" type="ordered locus">Nwi_1685</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Functions as a peptidoglycan terminase that cleaves nascent peptidoglycan strands endolytically to terminate their elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell inner membrane</location>
            <topology evidence="7">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the transglycosylase MltG family.</text>
    </comment>
    <dbReference type="EC" id="4.2.2.-" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314945.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1685"/>
    <dbReference type="EnsemblBacteria" id="ABA04946">
        <property type="protein sequence ID" value="ABA04946"/>
        <property type="gene ID" value="Nwi_1685"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1685"/>
    <dbReference type="eggNOG" id="COG1559">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025574_0_0_5"/>
    <dbReference type="OMA" id="IAMPGKA"/>
    <dbReference type="OrthoDB" id="708179at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008932">
        <property type="term" value="F:lytic endotransglycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08010">
        <property type="entry name" value="MltG_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02065">
        <property type="entry name" value="MltG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003770">
        <property type="entry name" value="MLTG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30518">
        <property type="entry name" value="PTHR30518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02618">
        <property type="entry name" value="YceG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00247">
        <property type="entry name" value="TIGR00247"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1 7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5 7 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="35"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="361"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="7">
        <location>
            <position position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA04946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="407" mass="44442" checksum="E7B930246D4887E1" modified="2005-10-11" version="1">MTERPPISPRSPRAALEPEQVPPPPKRSARVRSPLVVAGNAIITVLLIGMLGVGGVLVYGKQKIEAPGPLQEEKIVNIPSRAGMNDIADILQREGVIDSNRWAFIGGVFALKARADLKPGEYAFQKNASLREVIGTIVDGKVVQHPMTIPEGLTSEQIAARISENEIFSGTLREIPREGTLLPETYKFPRGTSRDQVIQRMQQTQKRVLAEIWERRASDVPVKTPEQLVTLASIIEKETGKADERSRVAAVFVNRLKQRMKLQSDPTIIYGLVGGKGTLGRPIKRSEIMHASPYNTYVIDGLPPGPIANPGRASLEAAANPARTRDLFFVADGSGGHSFTETYEQHQKNVARLRAMEKQIQNDAVEPIDDPPPAAAPTATESGATRDEPSSKPAPKPAPRTRTRPDR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-09-19" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS13</accession>
    <name>ANMK_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Anhydro-N-acetylmuramic acid kinase</fullName>
            <ecNumber evidence="1">2.7.1.170</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">AnhMurNAc kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">anmK</name>
        <name type="ordered locus">Nwi_1667</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the specific phosphorylation of 1,6-anhydro-N-acetylmuramic acid (anhMurNAc) with the simultaneous cleavage of the 1,6-anhydro ring, generating MurNAc-6-P. Is required for the utilization of anhMurNAc either imported from the medium or derived from its own cell wall murein, and thus plays a role in cell wall recycling.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1,6-anhydro-N-acetyl-beta-muramate + ATP + H2O = ADP + H(+) + N-acetyl-D-muramate 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:24952"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58690"/>
            <dbReference type="ChEBI" id="CHEBI:58722"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.170"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-sugar metabolism; 1,6-anhydro-N-acetylmuramate degradation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan recycling.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the anhydro-N-acetylmuramic acid kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.170" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314927.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SS13"/>
    <dbReference type="SMR" id="Q3SS13"/>
    <dbReference type="STRING" id="323098.Nwi_1667"/>
    <dbReference type="EnsemblBacteria" id="ABA04928">
        <property type="protein sequence ID" value="ABA04928"/>
        <property type="gene ID" value="Nwi_1667"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1667"/>
    <dbReference type="eggNOG" id="COG2377">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038782_3_0_5"/>
    <dbReference type="OMA" id="GQTIRHE"/>
    <dbReference type="OrthoDB" id="736294at2"/>
    <dbReference type="UniPathway" id="UPA00343"/>
    <dbReference type="UniPathway" id="UPA00544"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097175">
        <property type="term" value="P:1,6-anhydro-N-acetyl-beta-muramic acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006040">
        <property type="term" value="P:amino sugar metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009254">
        <property type="term" value="P:peptidoglycan turnover"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01270">
        <property type="entry name" value="AnhMurNAc_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005338">
        <property type="entry name" value="Anhydro_N_Ac-Mur_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30605">
        <property type="entry name" value="PTHR30605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03702">
        <property type="entry name" value="AnmK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000250016" description="Anhydro-N-acetylmuramic acid kinase">
        <location>
            <begin position="1"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01270"/>
        </source>
    </evidence>
    <sequence length="366" mass="38599" checksum="A97A4BDB16C53152" modified="2005-10-11" version="1">MMTAIGMMSGTSLDGVDVALIETDGERVTAFGPTGYRPYTNDERGLLREALAEAVNLTRRDARPGVIGMAEHAVTMAHSDAVTSFLARNGIRREDIDIVGFHGQTVLHRPGERLTVQIGDADALAKAIRVPVMHDFRAADVAAGGQGAPFVPAYHRALAQSLGRDGPICVVNIGGVSNVTYIDGADTLIACDTGPGNALLDDFMLRTTGEPFDRDGRLAQQGRPDADWIASALNHPFFALPPPKSLDRNDFASLALPDRPPADGAATLTAFTAAAIARIVPLLPKAPERWIVAGGGARNPIMLEMLREKVAPAPVERADALGWSIDAMEAQAFGYLAARGLKGLPLSYPATTGVPVPMTGGVVTRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS58</accession>
    <name>Q3SS58_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized phage protein (Possible DNA packaging)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1622</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314886.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1622"/>
    <dbReference type="EnsemblBacteria" id="ABA04883">
        <property type="protein sequence ID" value="ABA04883"/>
        <property type="gene ID" value="Nwi_1622"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1622"/>
    <dbReference type="eggNOG" id="ENOG5032WN3">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085951_6_4_5"/>
    <dbReference type="OMA" id="AQNHIER"/>
    <dbReference type="OrthoDB" id="1829390at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd08054">
        <property type="entry name" value="gp6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021146">
        <property type="entry name" value="Phafe_gp6_head-tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006450">
        <property type="entry name" value="Phage_HK022_Gp6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05135">
        <property type="entry name" value="Phage_connect_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01560">
        <property type="entry name" value="put_DNA_pack"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="106" mass="11943" checksum="B0A03B0639C4CFBD" modified="2005-10-11" version="1">MIATLADIKGQLNVTSDLDDTLLTRKISAAQDYLESWLGYKIETVYGGEDQEPVPPALVEAVLQLAAHWYENREATLVGVSAQQLPHGVSDIIREYRRWWSTPDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS70</accession>
    <name>Q3SS70_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1610</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04871">
        <property type="protein sequence ID" value="ABA04871"/>
        <property type="gene ID" value="Nwi_1610"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1610"/>
    <dbReference type="eggNOG" id="ENOG503141X">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1756929_0_0_5"/>
    <dbReference type="OMA" id="YRARICP"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229189" evidence="2">
        <location>
            <begin position="26"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="148" mass="15040" checksum="314D00961C5E6574" modified="2005-10-11" version="1">MKKNLIMIATVAALGSAVVSAPAQAQRFGPGLGVGLAVGALAAGAMASSFYGPYGYYGGGPYYGYGPGIYGAGWGGGWGGGWGGGWGPGFYGGGWGPGYYGGYRGYRGWGGRGGRGYYGGRGYYGGHRHWGGGRAYYGGRGWGGRGRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSA4</accession>
    <name>Q3SSA4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glutathione S-transferase</fullName>
            <ecNumber evidence="5">2.5.1.18</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_1576</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314843.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1576"/>
    <dbReference type="EnsemblBacteria" id="ABA04837">
        <property type="protein sequence ID" value="ABA04837"/>
        <property type="gene ID" value="Nwi_1576"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1576"/>
    <dbReference type="eggNOG" id="COG0625">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011226_14_4_5"/>
    <dbReference type="OMA" id="IAVWPWY"/>
    <dbReference type="OrthoDB" id="1560019at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="SSF47616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="3">
        <location>
            <begin position="46"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="4">
        <location>
            <begin position="135"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="257"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="266"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="290" mass="32750" checksum="5967E667CC7F70F8" modified="2005-10-11" version="1">MTDAPYMPPKVWTFDKENGGRFASVNRPTAGATHEKVLPVGKHPFQLYSRGTPNGQKVTIMFEELLARGHAGAEYDAWLIQIDGEQFGSGFVEVNPNSKIPALVDRSRPVPFRVFESGAILLHLADKFREFIPRATAARAECLSWLFWQMGSAPYLGGGFGHFYHYAPEKIEYAINRFAMETKRQMDVLDRRLKDNEYLAGNEYTIADMAVWPWYGGLAEGRLYGDSATFLDVQSYKNVQRWTAQIAARPAVKRGRIVNRTSGDPASQLHERHDASDFETRTQDKLAKQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSF3</accession>
    <name>Q3SSF3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Amino acid ABC transporter membrane protein 2, PAAT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_1527</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the binding-protein-dependent transport system permease family. HisMQ subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314794.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1527"/>
    <dbReference type="EnsemblBacteria" id="ABA04788">
        <property type="protein sequence ID" value="ABA04788"/>
        <property type="gene ID" value="Nwi_1527"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1527"/>
    <dbReference type="eggNOG" id="COG0765">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019602_16_1_5"/>
    <dbReference type="OMA" id="DTRLWGG"/>
    <dbReference type="OrthoDB" id="1523519at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010065">
        <property type="entry name" value="AA_ABC_transptr_permease_3TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043429">
        <property type="entry name" value="ArtM/GltK/GlnP/TcyL/YhdX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30614">
        <property type="entry name" value="PTHR30614"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01726">
        <property type="entry name" value="HEQRo_perm_3TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="141"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="233"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="259"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="293"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="332"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="368"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="472"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="297"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA04788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="506" mass="54338" checksum="E7F2A8189D98EE23" modified="2005-10-11" version="1">MTARPAPSFVRDDLVPERRAPVTTTNLLRFVQIRLFNSPGNALLTFVGVALLWLFVVPAVRFLFIDAAWEGQDRTSCLAADAGQSIGACWPFVQAKITQFIYGFYPEPERWRVNLTFLLAAVLLLPLLIPRLPAKRLNAGLFFVAFPVTAFFLLHGGGITGFGVSWSAGLLSSLADSVGDAGRQLAASGEAGEFGTILRPVGGAIASIGDVVNVLASPLMSLRDRLQGSGQPVWYDFGLTAAFISLLLFFFSGGFRAGWGALLTSLTAFAGMGLIIAVMPLDRGGLPVVDTRLWGGLLVTLVVSVTGIVSSIPIGIALALGRRSTLPLIRVFSIAFIEFWRGVPLITVLFFATYMLPLFLPGNFTVDGLVRVLIGIALFAGAYNAEVIRGGLAAVPGGQAEAAHALGLSYWKTTGLIVMPQAVRHVIPGLVNSFIALFKDTSLVSIVALFDLLGILRASFADPTWATPTTLFTGFAFTGLIYFAFCFSISRYSLFVEHRLNAHRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSG1</accession>
    <name>Q3SSG1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1519</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314786.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1519"/>
    <dbReference type="EnsemblBacteria" id="ABA04780">
        <property type="protein sequence ID" value="ABA04780"/>
        <property type="gene ID" value="Nwi_1519"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1519"/>
    <dbReference type="HOGENOM" id="CLU_996887_0_0_5"/>
    <dbReference type="OrthoDB" id="2193262at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229101" evidence="1">
        <location>
            <begin position="25"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="279" mass="30711" checksum="54C9982015D8A8D9" modified="2005-10-11" version="1">MNIRALNNSLIIGIAVLAPIQCLAANVPERFTGVWQAIENEDQACKASDWNSDRHTDTHIKIEAGLVRYHESECRFKSVSRPKVEFDSGAVHVAMTCDGEGERWTQTGIWQILPLGRNDTLAMVNVSKQRPGISLYRKCGGENAAVAPKNTTRAATGEAGDMRAAMREGRHCFAQKDEGVEFSLSVGSSGSASFSVDVANPRTKHLCSAKGTVAETAQGWRYVDKSQPTEVCQIDIEISDRIRFRFANESCERRYCGARASISSLEFSAQDKKESCSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSI3</accession>
    <name>Q3SSI3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1497</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04758">
        <property type="protein sequence ID" value="ABA04758"/>
        <property type="gene ID" value="Nwi_1497"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1497"/>
    <dbReference type="HOGENOM" id="CLU_2554851_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="82" mass="9178" checksum="4A3EA6DD807E186B" modified="2005-10-11" version="1">MVRHLAPERASANIVFAVVRDLPLRVTAQDLAMSKFALLLLEARTYAPLDVWRDHRLRCALLCQLSLRTISPSDPGRNPGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSM5</accession>
    <name>Q3SSM5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Outer membrane efflux protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1455</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the outer membrane factor (OMF) (TC 1.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314723.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1455"/>
    <dbReference type="EnsemblBacteria" id="ABA04716">
        <property type="protein sequence ID" value="ABA04716"/>
        <property type="gene ID" value="Nwi_1455"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1455"/>
    <dbReference type="eggNOG" id="COG1538">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012817_14_2_5"/>
    <dbReference type="OMA" id="QVERIYG"/>
    <dbReference type="OrthoDB" id="1533572at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003423">
        <property type="entry name" value="OMP_efflux"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02321">
        <property type="entry name" value="OEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="423" mass="45737" checksum="5EF2EECBD146C725" modified="2005-10-11" version="1">MLLKSAAHLAWAMALLISPWLMTPSYSQALTLRAALQRALAANPRLTAAGRDVGIATGRGIQAGMLINPEASYQQDNSLGSGNYRGTRSAETTLQISQLFELFGKRDARIAVGRAGVETAVIQRKAVRLEVLSETAVAFLSVLGAQRRIQILDEQIAAIDALTPLLQRRVEAGASSSAEIGRADVASALMKADRERIRARLASARRELAALMGDTSAKFKSVAGRLDDLGRPPSFQAVVAAIDANPQLMRWTAIYAQRNAELLVARLKPYPDVRVSAGWRHFNTTNDDAVILSLSVPIPVYDQNQGNILSAQENLAKTRAERESNRNTLIVLAGRAYDSLQGSLRELKVLREIAIPKSRSAADAIFEGYGQGRFSLLEVLDALANVAQARLREQEAQQNFHVGVATIEGLVGNPFALARGGVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSS8</accession>
    <name>Q3SSS8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1402</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314672.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1402"/>
    <dbReference type="EnsemblBacteria" id="ABA04663">
        <property type="protein sequence ID" value="ABA04663"/>
        <property type="gene ID" value="Nwi_1402"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1402"/>
    <dbReference type="eggNOG" id="COG0560">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061567_1_0_5"/>
    <dbReference type="OMA" id="WDFDKTL"/>
    <dbReference type="OrthoDB" id="1642828at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="307" mass="35463" checksum="3BCD3AEF5ADE42DF" modified="2005-10-11" version="1">MSRDLAQTPGRKSAAFRAYREYPHRTIAIIYDFDGTLSPLSMQEYTVFPELSIDPRTFWSEVKRVTKENEASEVLTYMRMMAERIVQGKLAINRERLGALGSHIEYFPGVETWFDRMNGFVRNQTLGYVRLNHYIVSSGLREILQGTSIAKHFRSIFASQYHYDSFGRPVFVDRVITDVSKTQYIFRINKGVENLAESVNDHMAEDERPIPFSNMIYLGDGDTDVPSMAVTRKNGGHAFAVYSRGEDPQKCEILFKANRVDAYFEANYSPNSRLEIFVKNLLKKMIAEIRYKAMLHLFKEERSTQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSS9</accession>
    <name>Q3SSS9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Propionyl-CoA carboxylase carboxyltransferase subunit</fullName>
            <ecNumber evidence="4">6.4.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1401</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.4.1.3" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314671.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1401"/>
    <dbReference type="EnsemblBacteria" id="ABA04662">
        <property type="protein sequence ID" value="ABA04662"/>
        <property type="gene ID" value="Nwi_1401"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1401"/>
    <dbReference type="eggNOG" id="COG4799">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018822_6_0_5"/>
    <dbReference type="OMA" id="YAYAECT"/>
    <dbReference type="OrthoDB" id="886663at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004658">
        <property type="term" value="F:propionyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="3">
        <location>
            <begin position="261"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="510" mass="55681" checksum="E8D6923A6ECF6C4B" modified="2005-10-11" version="1">MKNIREALDARRAAAKLGGGEKRIEAQHRRGKLTARERIEILLDKGSFEEFDMFVEHRSVEFGMEKSKVPGDGVVTGWGTVNGRTIFVFAKDFTVFGGSLSETHALKITKIQDLALKARAPIIGLYDAGGARIQEGVAALAGYSYVFRRNVQASGVIPQISVIMGPCAGGDVYSPAMTDFIFMVKNTSYMFVTGPDVVKTVTGEAVTSEELGGASVHTTKSSIADGAFENDVEALLQMRRLIDFLPGNNTDGVPEWPSFDEVERTDDSLDTLVPDNPNKPYDIKELILKVVDEGDFFEIAETFAKNIVTGFGRIAGRTVGFVANQPMVLAGVLDSDASRKAARFVRFCDAFNIPIVTFVDVPGFLPGTAQEYGGLIKHGAKLLFAYSQCTVPLVTVITRKAYGGAFDVMASKEIGADINYAWPTAQIAVMGAKGAVEIIFRNDIGDEEKIAARTREYEDRFLSPFVAAERGYIDDVILPSSTRRRIARALAMLRDKHVEVLAKKHDNLPL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSU4</accession>
    <name>RS13_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsM</name>
        <name type="ordered locus">Nwi_1386</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits; these bridges are implicated in subunit movement. Contacts the tRNAs in the A and P-sites.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Forms a loose heterodimer with protein S19. Forms two bridges to the 50S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009797115.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSU4"/>
    <dbReference type="SMR" id="Q3SSU4"/>
    <dbReference type="STRING" id="323098.Nwi_1386"/>
    <dbReference type="EnsemblBacteria" id="ABA04647">
        <property type="protein sequence ID" value="ABA04647"/>
        <property type="gene ID" value="Nwi_1386"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1386"/>
    <dbReference type="eggNOG" id="COG0099">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103849_1_2_5"/>
    <dbReference type="OMA" id="YRGLRHK"/>
    <dbReference type="OrthoDB" id="1772647at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.910.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01315">
        <property type="entry name" value="Ribosomal_S13_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027437">
        <property type="entry name" value="30s_Rbsml_prot_S13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001892">
        <property type="entry name" value="Ribosomal_S13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_S13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019980">
        <property type="entry name" value="Ribosomal_S13_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018269">
        <property type="entry name" value="Ribosomal_S13_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871:SF1">
        <property type="entry name" value="PTHR10871:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00416">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002134">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="SSF46946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03631">
        <property type="entry name" value="uS13_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00646">
        <property type="entry name" value="RIBOSOMAL_S13_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50159">
        <property type="entry name" value="RIBOSOMAL_S13_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000230532" description="30S ribosomal protein S13">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="95"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="122" mass="13842" checksum="8A76AC6F4C4254CA" modified="2005-10-11" version="1">MARIAGVNIPTNKRVVIALQYIHGIGQKNAAEIIEKVKIPADRRVSQLTDQEVLQIREVIDRDYLVEGDLRREVGINIKRLMDLGCYRGLRHRRGLPVRGQRTHTNARTRKGPAKSIAGKKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSX0</accession>
    <name>RS7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsG</name>
        <name type="ordered locus">Nwi_1360</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, probably blocks exit of the E-site tRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Contacts proteins S9 and S11.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314638.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSX0"/>
    <dbReference type="SMR" id="Q3SSX0"/>
    <dbReference type="STRING" id="323098.Nwi_1360"/>
    <dbReference type="EnsemblBacteria" id="ABA04621">
        <property type="protein sequence ID" value="ABA04621"/>
        <property type="gene ID" value="Nwi_1360"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1360"/>
    <dbReference type="eggNOG" id="COG0049">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072226_1_1_5"/>
    <dbReference type="OMA" id="NVMPHVE"/>
    <dbReference type="OrthoDB" id="1540940at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00480_B">
        <property type="entry name" value="Ribosomal_S7_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_S5/S7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005717">
        <property type="entry name" value="Ribosomal_S7_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020606">
        <property type="entry name" value="Ribosomal_S7_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023798">
        <property type="entry name" value="Ribosomal_S7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_S7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="PTHR11205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002122">
        <property type="entry name" value="RPS7p_RPS7a_RPS5e_RPS7o"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="SSF47973"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01029">
        <property type="entry name" value="rpsG_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00052">
        <property type="entry name" value="RIBOSOMAL_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000226511" description="30S ribosomal protein S7">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00480"/>
        </source>
    </evidence>
    <sequence length="156" mass="17661" checksum="7A00E2DD0B8491D3" modified="2005-10-11" version="1">MSRRHSAEKREVLPDPKFGNVVITKFMNSIMYAGKKSVAESIVYGALDLIEAKTKQGPLTVFEQALENVMPTIEVRSRRVGGATYQVPVEVRSTRRQALGIRWLITAARGRNEKTMTERLSAELLDASNNRGSAVKKREDVHKMAEANRAFSHYRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSY6</accession>
    <name>Q3SSY6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein translocase subunit SecE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">secE</name>
        <name evidence="8" type="ordered locus">Nwi_1344</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Essential subunit of the Sec protein translocation channel SecYEG. Clamps together the 2 halves of SecY. May contact the channel plug during translocation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the Sec protein translocase complex. Heterotrimer consisting of SecY, SecE and SecG subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. Interacts with SecDF, and other proteins may be involved. Interacts with SecA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell inner membrane</location>
            <topology evidence="7">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the SecE/SEC61-gamma family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314623.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1344"/>
    <dbReference type="EnsemblBacteria" id="ABA04605">
        <property type="protein sequence ID" value="ABA04605"/>
        <property type="gene ID" value="Nwi_1344"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1344"/>
    <dbReference type="eggNOG" id="COG0690">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113663_4_1_5"/>
    <dbReference type="OMA" id="FFFAADQ"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043952">
        <property type="term" value="P:protein transport by the Sec complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00422">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005807">
        <property type="entry name" value="SecE_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038379">
        <property type="entry name" value="SecE_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001901">
        <property type="entry name" value="Translocase_SecE/Sec61-g"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33910">
        <property type="entry name" value="PTHR33910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00584">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01650">
        <property type="entry name" value="SECETRNLCASE"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00964">
        <property type="entry name" value="secE_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA04605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="63" mass="7365" checksum="AF023A492EA8A2D4" modified="2005-10-11" version="1">MAFSPFKFLQEVRTETAKVTWPSRRETTVTTIMVFVMVALASIFFFFTDQIIRVFITFVLGLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST30</accession>
    <name>Q3ST30_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Macro domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1300</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1300"/>
    <dbReference type="EnsemblBacteria" id="ABA04561">
        <property type="protein sequence ID" value="ABA04561"/>
        <property type="gene ID" value="Nwi_1300"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1300"/>
    <dbReference type="eggNOG" id="COG2110">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054419_3_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="SSF52949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Macro" evidence="1">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="136" mass="15447" checksum="DD8C1B314A14B714" modified="2005-10-11" version="1">MSQNLRSRDPGTVSIHLTTDKSTAQTLVNTVNCVGVMGKGIALEFKKRQPDMFDAYKRICDKKLLTPGKLWLWRGNEHLTLNFPTKIHWRNPSKLEWIESGLRKFVENYKALGISEISFPRLGCGNGGLDWDDVAQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-09-02" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST43</accession>
    <name>SYA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Alanine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Alanyl-tRNA synthetase</fullName>
            <shortName evidence="1">AlaRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">alaS</name>
        <name type="ordered locus">Nwi_1287</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)</text>
            <dbReference type="Rhea" id="RHEA:12540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:9923"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78497"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.7" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314574.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ST43"/>
    <dbReference type="SMR" id="Q3ST43"/>
    <dbReference type="STRING" id="323098.Nwi_1287"/>
    <dbReference type="EnsemblBacteria" id="ABA04548">
        <property type="protein sequence ID" value="ABA04548"/>
        <property type="gene ID" value="Nwi_1287"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1287"/>
    <dbReference type="eggNOG" id="COG0013">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004485_1_1_5"/>
    <dbReference type="OMA" id="YHHTMFE"/>
    <dbReference type="OrthoDB" id="91428at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006419">
        <property type="term" value="P:alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00036_B">
        <property type="entry name" value="Ala_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002318">
        <property type="entry name" value="Ala-tRNA-lgiase_IIc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018162">
        <property type="entry name" value="Ala-tRNA-ligase_IIc_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018164">
        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023033">
        <property type="entry name" value="Ala_tRNA_ligase_euk/bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00980">
        <property type="entry name" value="TRNASYNTHALA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101353">
        <property type="entry name" value="SSF101353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="SSF55186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00344">
        <property type="entry name" value="alaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000347699" description="Alanine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="564"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="568"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="678"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="682"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00036"/>
        </source>
    </evidence>
    <sequence length="891" mass="95706" checksum="F4E49910A5ED5A38" modified="2005-10-11" version="1">MSGVNDIRSAFLNYFAANGHQIVPSSPLVPRNDPTLMFTNAGMVQFKNVFTGVEKRPYQRATTSQKCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYFKDRAIELAWKLVTQEFGLPKDRLTATVYIDDDGAFDLWKKIAGLPESRIIRIAGSDNFWQMGDTGPCGPCSEIFYDHGDRIPGGPPGSPEQDGDRFVEIWNLVFMQYEKLPDGSRPNLPKPSIDTGAGLERVAAVLQGKHDNYDIDLFVALIRAAADLTGADPQGPMKASLRVIADHLRASSFLIADGVLPSNEGRGYVLRRIMRRAMRHAQLLGAREPLMWRLVGVLVREMGEAFPELQRARPLIEETLRLEETRFRKTLERGLSILDEKSASLKQGDMFDGETAFTLYDTYGFPLDLTQDALRARGIGVDLASFTDAMEQQKAKARASWSGSGEAAAESIWFPLREKLGATEFLGYETEAAEGVVAALVKDGEEADTLKAGESGAIVLNQTPFYGESGGQVGDTGFLIADGVRFRVTDTQKKAGDLFVHLGTVEQGVLAPGMALALEVDHDRRSAIRANHSATHLLHEALRQVLGDHIAQRGSLVAPERLRFDFAHNKPISADELRCIEDIANDVVLENGEVTTRLMAVDDAREAGARALFGEKYGDEVRVVSMGGTDRHGAASNALGWSVELCGGTHVKRTGDIGLISVVGESAVASGVRRIEALTGHQARHHANHAIQLAKTAAGELRTSLDDMPARIASLMEERKKLERELSEARKKLAMGGAASASSGATTGVRDVGGIKLMARSVEGIEIKDLKSLADQGKKQLGSGVVALVATSGDGKASVVVGVTPDLVTRFSAVDLVRKASEVLGGKGGGGKPDMAQAGGPDGAKAGAALDAIAAAMGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST44</accession>
    <name>Q3ST44_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aminomethyltransferase</fullName>
            <ecNumber evidence="3">2.1.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Glycine cleavage system T protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_1286</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6S)-5,6,7,8-tetrahydrofolate + (R)-N(6)-(S(8)-aminomethyldihydrolipoyl)-L-lysyl-[protein] = (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + (R)-N(6)-dihydrolipoyl-L-lysyl-[protein] + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16945"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:10492"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="2.1.2.10"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GcvT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.10" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314573.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1286"/>
    <dbReference type="EnsemblBacteria" id="ABA04547">
        <property type="protein sequence ID" value="ABA04547"/>
        <property type="gene ID" value="Nwi_1286"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1286"/>
    <dbReference type="eggNOG" id="COG0404">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007884_10_0_5"/>
    <dbReference type="OMA" id="MPVQYPA"/>
    <dbReference type="OrthoDB" id="282830at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004047">
        <property type="term" value="F:aminomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006546">
        <property type="term" value="P:glycine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006223">
        <property type="entry name" value="GCS_T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028896">
        <property type="entry name" value="GCST/YgfZ/DmdA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013977">
        <property type="entry name" value="GCV_T_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006222">
        <property type="entry name" value="GCV_T_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029043">
        <property type="entry name" value="GcvT/YgfZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43757">
        <property type="entry name" value="PTHR43757"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01571">
        <property type="entry name" value="GCV_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08669">
        <property type="entry name" value="GCV_T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101790">
        <property type="entry name" value="SSF101790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00528">
        <property type="entry name" value="gcvT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GCV_T" evidence="7">
        <location>
            <begin position="16"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="GCV_T_C" evidence="8">
        <location>
            <begin position="297"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA04547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="383" mass="40277" checksum="F3E39B63D9D1AFB0" modified="2005-10-11" version="1">MVTHVSDAPTLQKTPLHALLLERGGKMVAFAGYDMPVQYASGVLKEHLHTRAKAGLFDVSHMGQIALRPKSGDVRDAALALERLVPQDIVAVAPGRQRYAQFTNPAGGLLDDLMVANFGDHLFLVVNGACKAADEAHLREHLSDACAIEVLTDRALIALQGPKAASVLAKKCPEVPAMKFMEAGPRQVGGVACFVSRSGYTGEDGYEISIPAARAEDVVSALLDDPDVAPVGLGARDSLRLEAGLCLYGHDIGATTTPVEAALEWSVQKSRRSGGVRAGGFPGANVILPQFEQGAARRRVGLRPEGRAPVREGAPLFADAASADSIGAVTSGGFGPSLNAPIAMGYLPVSHAATGGLVYAEVRGQRLPLRICAMPFVPHNYKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST65</accession>
    <name>Q3ST65_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acriflavin resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1265</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314554.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1265"/>
    <dbReference type="EnsemblBacteria" id="ABA04526">
        <property type="protein sequence ID" value="ABA04526"/>
        <property type="gene ID" value="Nwi_1265"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1265"/>
    <dbReference type="eggNOG" id="COG0841">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002755_1_2_5"/>
    <dbReference type="OMA" id="GQDFSIM"/>
    <dbReference type="OrthoDB" id="145346at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2090.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027463">
        <property type="entry name" value="AcrB_DN_DC_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001036">
        <property type="entry name" value="Acrflvin-R"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32063">
        <property type="entry name" value="PTHR32063"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00873">
        <property type="entry name" value="ACR_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00702">
        <property type="entry name" value="ACRIFLAVINRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82714">
        <property type="entry name" value="SSF82714"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="336"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="362"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="388"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="433"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="465"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="532"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="859"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="911"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="958"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="989"/>
            <end position="1015"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1044" mass="113016" checksum="11E18510ED0FF705" modified="2005-10-11" version="1">MNDSLSAPFIRYPIGTSLLMAGILFIGLVAFPLLPVAPLPQVDFPTIQVQANLPGASPETMASSVAQPLERQMAQIPGVAQMTSTSSIGSTSITVQFDLNRNIDGAANDIQRAINAAGGQLPKNLPSPPTYRKVNPADSPIMLLSATSDTLPLTTVSDNTDTQLAQQISQISGVGQVIIGGQQKPSIRIQIDPAKLVAKGLSLEDVRNQISVTTVNSPKGSIDGGTRSYTIYANDQLTLSKDWGDVIIAYRNGAPLRIRDIGQAVTGPEDNKQAAWANGKRGVFLIIFKQPGANVIDTVDRIKAELPRLIAAIPPAIKIDLISDRTTTIRAAVEDVQFTLLITVVLVVLVIFLFLRNFWATIIPSVTVPLALLGACALMWPFDYSLNNLSLMALTISVGFVVDDAIVVLENISRHVEKGEEPMAAALKGAREIGFTILSISISLVAVLIPLLLMGGIIGRLFREFAVTLAMAILVSLVVSLTLTPMMASRFLRRHDSVRHGRLYRLSERGFDAMLDAYRRGLDLVLRWRRTTLLVFFMTLGLSVYLFVIIPKGFFPQQDNGMILGTSEAAQDISFAEMKKKQEELGRIVLADPAVHSIAMFIGGGGSALNNGRMYVTLKPLAERDASAQQVIARLRPKLDQVEGARQFLQASQDVRLGGRATRTQFEYTLQDADLDELNTWAPKVLKKMQALPELRDVATDQQTGGTTLTLTIDRDAASRYGISPQLISDTLYDSFGQRQVAQYFTQLNTYRVILEILPELQGRLDTLDRIYLKSPLTGEQVPLSVFASWTTVPVQPLSISHQGQFPAITISFNLAENASLGDATAAVHNAMRELGAPGTLNGSFQGTAQAFQQSLGTVPLLILAALVVVYLILGILYESYIHPITILSTLPSAGVGALATLMLFGYDFSLIALIGIILLIGIVKKNGIMMVDFAIAAEREEHLPPEESIRKAALLRFRPIMMTTMAAMLGGVPLMLGTGAGSEIRQPLGYAMVGGLLVSQALTLFTTPVVYLYLDQLSNWLSGSGRPGDRNLSSHALIKEAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST67</accession>
    <name>Q3ST67_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, XRE family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1263</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314552.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1263"/>
    <dbReference type="EnsemblBacteria" id="ABA04524">
        <property type="protein sequence ID" value="ABA04524"/>
        <property type="gene ID" value="Nwi_1263"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1263"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066192_21_2_5"/>
    <dbReference type="OMA" id="NRADYAP"/>
    <dbReference type="OrthoDB" id="1933795at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="12"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="117" mass="12961" checksum="0BE229D1D504FC8D" modified="2005-10-11" version="1">MTSGREKFGAFIRREREAREIGLREMAKKIGVSPTYMSKVERDEFTPPTEDKVRAIAQILECDPDELLAMAGRVPSDLADIIKRQPVEMSALLRTANGLSADDLARLSREAGKAKGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STA7</accession>
    <name>Q3STA7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF2059 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1222</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314513.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1222"/>
    <dbReference type="EnsemblBacteria" id="ABA04484">
        <property type="protein sequence ID" value="ABA04484"/>
        <property type="gene ID" value="Nwi_1222"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1222"/>
    <dbReference type="eggNOG" id="COG3184">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128149_0_0_5"/>
    <dbReference type="OMA" id="GDGMAKI"/>
    <dbReference type="OrthoDB" id="1604355at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018637">
        <property type="entry name" value="DUF2059"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09832">
        <property type="entry name" value="DUF2059"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229223" description="DUF2059 domain-containing protein" evidence="1">
        <location>
            <begin position="31"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2059" evidence="2">
        <location>
            <begin position="100"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="174" mass="18850" checksum="670FCA1A74B2B335" modified="2005-10-11" version="1">MRGLSRFLPARFLPAACLAVALALPAVAQAQQAGQPSPAAIASAKELLTLKNAAGMYANAVPGIVERTKNTLLQSNLNYQKDLNEVALVVAKSLAGREKEIGDGMAKIYAGHFTEQELKDLVTFYKSPLGKKLIAQEPKAIEASMGYMNQWAQQFASVVNGEFRAEMRKRGKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STD0</accession>
    <name>Q3STD0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">ATP:glutamine synthetase adenylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">ATase</fullName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName evidence="3">Glutamine synthetase adenylyl-L-tyrosine phosphorylase</fullName>
                <ecNumber evidence="3">2.7.7.89</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">Adenylyl removase</fullName>
                <shortName evidence="3">AR</shortName>
                <shortName evidence="3">AT-N</shortName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="3">Glutamine synthetase adenylyl transferase</fullName>
                <ecNumber evidence="3">2.7.7.42</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">Adenylyl transferase</fullName>
                <shortName evidence="3">AT</shortName>
                <shortName evidence="3">AT-C</shortName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="3" type="primary">glnE</name>
        <name evidence="6" type="ordered locus">Nwi_1199</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in the regulation of glutamine synthetase GlnA, a key enzyme in the process to assimilate ammonia. When cellular nitrogen levels are high, the C-terminal adenylyl transferase (AT) inactivates GlnA by covalent transfer of an adenylyl group from ATP to specific tyrosine residue of GlnA, thus reducing its activity. Conversely, when nitrogen levels are low, the N-terminal adenylyl removase (AR) activates GlnA by removing the adenylyl group by phosphorolysis, increasing its activity. The regulatory region of GlnE binds the signal transduction protein PII (GlnB) which indicates the nitrogen status of the cell.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>[glutamine synthetase]-L-tyrosine + ATP = [glutamine synthetase]-O(4)-(5'-adenylyl)-L-tyrosine + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18589"/>
            <dbReference type="Rhea" id="RHEA-COMP:10660"/>
            <dbReference type="Rhea" id="RHEA-COMP:10661"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:83624"/>
            <dbReference type="EC" id="2.7.7.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>[glutamine synthetase]-O(4)-(5'-adenylyl)-L-tyrosine + phosphate = [glutamine synthetase]-L-tyrosine + ADP</text>
            <dbReference type="Rhea" id="RHEA:43716"/>
            <dbReference type="Rhea" id="RHEA-COMP:10660"/>
            <dbReference type="Rhea" id="RHEA-COMP:10661"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:83624"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.7.89"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GlnE family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.89" evidence="3"/>
    <dbReference type="EC" id="2.7.7.42" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314490.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1199"/>
    <dbReference type="EnsemblBacteria" id="ABA04461">
        <property type="protein sequence ID" value="ABA04461"/>
        <property type="gene ID" value="Nwi_1199"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1199"/>
    <dbReference type="eggNOG" id="COG1391">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006233_0_0_5"/>
    <dbReference type="OMA" id="EFMVQYA"/>
    <dbReference type="OrthoDB" id="427701at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008882">
        <property type="term" value="F:[glutamate-ammonia-ligase] adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047388">
        <property type="term" value="F:[glutamine synthetase]-adenylyl-L-tyrosine phosphorylase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000820">
        <property type="term" value="P:regulation of glutamine family amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00802">
        <property type="entry name" value="GlnE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023057">
        <property type="entry name" value="GlnE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005190">
        <property type="entry name" value="GlnE_rpt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013546">
        <property type="entry name" value="PII_UdlTrfase/GS_AdlTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30621">
        <property type="entry name" value="PTHR30621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08335">
        <property type="entry name" value="GlnD_UR_UTase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03710">
        <property type="entry name" value="GlnE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GlnE" evidence="4">
        <location>
            <begin position="66"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="GlnD_UR_UTase" evidence="5">
        <location>
            <begin position="328"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="GlnE" evidence="4">
        <location>
            <begin position="582"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="domain" description="GlnD_UR_UTase" evidence="5">
        <location>
            <begin position="861"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="region of interest" description="Adenylyl removase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Adenylyl transferase" evidence="3">
        <location>
            <begin position="483"/>
            <end position="987"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA04461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="987" mass="109361" checksum="9C6626A4DFA80D83" modified="2005-10-11" version="1">MISSVKDDADPASLAARLADGPKLFAPFEAERRLEDWLSDLTPQQSDGIRTLGGTFPHVKAILLGIAESSPYLFDLMRSDPGRMLRLLGADPESHLERLIAGIWEAAEAADEAEAMRRLRTVKMEAALLIALCDIGGVWPVMRITAGLTDIAVAAVRSALRFLLRQETGRGRLNPPDIDHPEEASGFVVLAMGKMGAGELNYSSDIDLIVFFDLAAPTLVSGIEAQPFFVRLTQGLARLLQQRTGDGYVFRVDLRLRPDPASTQVAVSTAAALDYYEREGRTWERAAMIKARPCAGDRKAGETMIAEISPFVWRKHLDFAALAEVHDMKRQMQTFRGQSEIAVEGHNVKIGRGGIREIEFFAQTQQLVAGGRHPELRVRPTLEALNILASSDWITHQARDELTAAYLFLRRVEHRIQMVADEQTHVLPADAEAMERFARFLGFEDRAGFATELLAHLNCVQGHYSRLFEGDPAGTINIPVADYRAGPDDPRLLDHLAKLGFKQPRMVADSIRQWMAGGYRALKAEATRTAFEAFVPTLIDGLARAEEPDSAVMAFDRFLQALQRGGRLISLLSQNRDLVALVALVLGAAPRLADMLARRPQIMDGLIDPRFFGAMPDRLELSARLSATLQDADSYEEFLDRIRLFGQESLFLIGTRILSGTVSARQAGHVFADVAEGIVDTVHGLVKQQFADQYGRISGQETAIVAMGKLGGREMTASSDLDLILLYDFDSDHPDSDGARPLHGAQYFARFTQRLISAFTTRTNYGVLYEVDMRLRPSGRSGPVASHIDSFAEYQEHEAWTWEHMALTRARVISSSPEFRARIERVIRDVLTRPRDGRLIASDVAEMRRAIALEKGEDDIWDLKNAAGGMVDIDFIAQYLQLVHAADMPDILSIDTQQALDNAQRLGVLSLPDAEILRPAARLYHDLTQILRLCVSEKFKPETAGDDLLPVLARAGDAPDFSSLEARVRETQADVRAVFLRLLEGKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STF5</accession>
    <name>Q3STF5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phage major tail protein, TP901-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1174</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314465.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1174"/>
    <dbReference type="EnsemblBacteria" id="ABA04436">
        <property type="protein sequence ID" value="ABA04436"/>
        <property type="gene ID" value="Nwi_1174"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1174"/>
    <dbReference type="eggNOG" id="COG5437">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151316_0_0_5"/>
    <dbReference type="OMA" id="FAGEHNG"/>
    <dbReference type="OrthoDB" id="1555559at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022344">
        <property type="entry name" value="GTA_major-tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011855">
        <property type="entry name" value="Phgtail_TP901_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06199">
        <property type="entry name" value="Phage_tail_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01996">
        <property type="entry name" value="MTP1FAMILY"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02126">
        <property type="entry name" value="phgtail_TP901_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="135" mass="14290" checksum="7F707CF17F09C55B" modified="2005-10-11" version="1">MGAQKGKDLLLKMSDGSSFVTVAGLRSRRIAFNAEIVDVTDAESTDRWRELLAGAGVRRASVSGRGLFKDAASDALVRQAFFDGAIKSCQVVVPDFGAIEGMFQIASLEFAGEHNGEVTFDLALESAGALTFTAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STH2</accession>
    <name>Q3STH2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ISRm2011-2 transposase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1157</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04419">
        <property type="protein sequence ID" value="ABA04419"/>
        <property type="gene ID" value="Nwi_1157"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1157"/>
    <dbReference type="eggNOG" id="COG3335">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056788_10_0_5"/>
    <dbReference type="OMA" id="DTXRGKS"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0814">Transposable element</keyword>
    <feature type="domain" description="DDE_3" evidence="2">
        <location>
            <begin position="2"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="168" mass="19210" checksum="2752748EB9907115" modified="2005-10-11" version="1">MFIDETWTKTNMAPLRGWAPRGQRIRAKVPHGRWQTMTFMAALRHDRITAPWFIEGPINGEAFLLYIEKVLVPTLRHGDIVIMDNLGSHKASAVRRVIRAAGARLFYLPKYSPDLNPIEQFFAKFKHWLRKAAQRTTEAVYNAIAPILETVAPAECANYFVNAGYNQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STJ1</accession>
    <name>Q3STJ1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_1138</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1138"/>
    <dbReference type="EnsemblBacteria" id="ABA04400">
        <property type="protein sequence ID" value="ABA04400"/>
        <property type="gene ID" value="Nwi_1138"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1138"/>
    <dbReference type="eggNOG" id="COG0784">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_51_5"/>
    <dbReference type="OMA" id="RWGQFFV"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="9">
        <location>
            <begin position="120"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="510"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="8">
        <location>
            <begin position="769"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="4">
        <location>
            <position position="820"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA04400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="888" mass="96222" checksum="C09255E654AA913E" modified="2005-10-11" version="1">MAGNEPNEALALRAPIQALRTRRRRIPRPPRQAPPNSMTPDPDIDSPQVGQEPARRSGSILLVLLVAGAIVTAAVAFMALGRTEAQPYILALLALLAMVGLFTLFAFAAGIVRFADRTVDDPLMRPIADHAFDGIAVTDSGGHIVYANSAYLTLTGAASMQDVRPVERVFIGNPDVSEAVFRLLKAAREGKRQQEEVRVAAADGTNGRWLRLRVRPLAQAKRYARYAVWSIADITRDRERQEDVFQELRHAIEYLDHAPCGFFSVNQSGDLVYVNATLANWLDHDLAEIGSGGLKLTDIVSGDGAALLTSIIAAPGEVRTEVFDIDLRMRNGKTMPARLYHKLAFGADGVPGPSRTLVVSRARDERVDPQRAAEVRFMRFFDHTPMAIATVDKSGVVGRANARFAKLAQSLSPDGAVARSILAAVNERDRGQLSAAILAAADGQGDIPPVEVMLSGARERWGQFFVTAVEDDHDAEAAIVYMLETTERRTLESQINQSQKMESVGQLAGGIAHDFNNVLSAIMMANDFLLNAHKPTDPSFQDIMQIKQNANRAAALVRQLLAFSRRQTLQPKVLDLGDTLSDIGMLLKRLIGEKVTFPGVVHGRDLWPVKADVSQFEQVIVNLVVNARDAMPDGGKLAIRTTNVAAAEVATFGHKGLPPADYVLVEISDTGTGIPPDIVDKIFEPFFSTKEVGKGTGLGLSTVYGIVKQTGGFIYVDSRTGEGTTFRIFLPRHHPQAEALPASPGLLGEAKEPSSTAKPVNTDLTGQGTILLVEDEEGLRSLNARGLRSRGYSVIEASNGIEALEALDEKDGAVDLVVSDVVMPEMDGPTLLRKMRVRNPNLKIIFVSGYAEEAFDKSLPENEQFAFLAKPFALSALIAKVKETMTAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STQ2</accession>
    <name>Q3STQ2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DegT/DnrJ/EryC1/StrS aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1077</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the DegT/DnrJ/EryC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314373.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1077"/>
    <dbReference type="EnsemblBacteria" id="ABA04339">
        <property type="protein sequence ID" value="ABA04339"/>
        <property type="gene ID" value="Nwi_1077"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1077"/>
    <dbReference type="eggNOG" id="COG0399">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033332_6_1_5"/>
    <dbReference type="OMA" id="PGDAVFC"/>
    <dbReference type="OrthoDB" id="1006364at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00616">
        <property type="entry name" value="AHBA_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000653">
        <property type="entry name" value="DegT/StrS_aminotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30244">
        <property type="entry name" value="PTHR30244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01041">
        <property type="entry name" value="DegT_DnrJ_EryC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000390">
        <property type="entry name" value="PLP_StrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR000390-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000390-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="382" mass="40513" checksum="5D12CB5E5374DF97" modified="2005-10-11" version="1">MQQHMPSPAISFIDIAEQRRRLGKKIDDAIAGVLTHCQFINGPEVARLEADLAAFSGAKHVVACASGTDALLMVLLAKKVGPGDAVICPTFTFCATGEVVALLGATPVFVDVDETTFNIDIASLKNGIAVARSQGLRPRAIIPVDLFGQPADHDAVAAVAAAEGLFVLDDAAQGFGASYKGQRLGRFGLATATSFFPAKPLGCYGDGGAIFTDDTDLAETLRSVRVHGQGADKYDNVRLGLTGRIDTMQAAVLIEKLKIFPEEIAARDAVARRYNEGLGQVAIVPRVAPDRTSVWAQYTIRAPNGRRDALASALKAQGIPTAIYYLRSLHQQTAYRSFPVAGGGLAVSERLSTEVISLPMHPYLDESTQGRIIEAVRAALAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STU0</accession>
    <name>Q3STU0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1039</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04301">
        <property type="protein sequence ID" value="ABA04301"/>
        <property type="gene ID" value="Nwi_1039"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1039"/>
    <dbReference type="HOGENOM" id="CLU_2369990_0_0_5"/>
    <dbReference type="OMA" id="ETAAHIN"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="95" mass="11036" checksum="2B472C18689DE0E1" modified="2005-10-11" version="1">MTGRPCTVPGVQNKPDTANPSLEKRKENAMPHRRRYKQTRPFQDRLFDFLAEARQKAEESPDGIEREKMIKRIESAKTAARIDAWANSPKMQRPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STZ4</accession>
    <name>Q3STZ4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0985</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0985"/>
    <dbReference type="EnsemblBacteria" id="ABA04247">
        <property type="protein sequence ID" value="ABA04247"/>
        <property type="gene ID" value="Nwi_0985"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0985"/>
    <dbReference type="eggNOG" id="ENOG5031422">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2288583_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="73"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="101" mass="11149" checksum="D9860A552E52D12B" modified="2005-10-11" version="1">MSIDQGRDGMLQTRPPTYPRYPRALQIVLQTLFTFVGESEATSTGVSQMSFEAGEAVIDEIVASCDGDLRGALMALLMVNERLENELQRLYAAVRDGNTLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU29</accession>
    <name>Q3SU29_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aspartate/glutamate/uridylate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0950</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314253.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0950"/>
    <dbReference type="EnsemblBacteria" id="ABA04212">
        <property type="protein sequence ID" value="ABA04212"/>
        <property type="gene ID" value="Nwi_0950"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0950"/>
    <dbReference type="eggNOG" id="COG0528">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1008069_0_0_5"/>
    <dbReference type="OMA" id="YHHHEFP"/>
    <dbReference type="OrthoDB" id="1043239at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030669">
        <property type="entry name" value="MoSto_subunit_alpha/beta"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039097">
        <property type="entry name" value="MoSto_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AA_kinase" evidence="1">
        <location>
            <begin position="41"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="277" mass="29007" checksum="1D52F98A4EECEF88" modified="2005-10-11" version="1">MADTNDKIKHVASPLARQTLLDGALTAPVAGKRPIRLLPWLQVVKIGGRSIMDRGRDAILPIVDEIRKLLPEHRLLILTGAGIRARHVYGVGLDLGLPVGSLAPLAASEAGQNGHILGALLAPDGVSYIEHPTIASQLAIHLSAARAVVGSAFPPYHHHEFPGSRIPPHRADTGAFLLADALGAAGVTIVEDVDGVYTADPSGPDGKKAELLRETSAADLAKFKGTLPIDTALVEVMATARHIERVQVVNGLVPGRLTAALRGEHVGTIIHTGAHQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU61</accession>
    <name>Q3SU61_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">WD-40 repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0918</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314221.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0918"/>
    <dbReference type="EnsemblBacteria" id="ABA04180">
        <property type="protein sequence ID" value="ABA04180"/>
        <property type="gene ID" value="Nwi_0918"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0918"/>
    <dbReference type="eggNOG" id="COG2319">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067065_1_0_5"/>
    <dbReference type="OMA" id="EPALHGW"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024977">
        <property type="entry name" value="Apc4_WD40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12894">
        <property type="entry name" value="ANAPC4_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="SSF50978"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ANAPC4_WD40" evidence="2">
        <location>
            <begin position="276"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="337" mass="35530" checksum="B332916564A175CB" modified="2005-10-11" version="1">MKDTAPKQHAMASIGERVRPVTIGSPVAALHFIGDRAAFVGDEETVTLVSGDGEKAAVTVHGGAILSAVSDGKRIVTGGDDGKVVALDMKGESSVLVTDAKRRWIDNIAVHPDGTLAWSAGKTAYVRGIKGEEKSIDAPSSVGGLAFAPKGMRLALAHYNGVSLWFPNMKAAPERLEWAGSHLGVTFSPDGRFLITSMHEPALHGWRLVDVQHMQMTGYPSRVKSLSWGMGGKLLATSGADRVVVWPFASKDGPMDKEPAMLAPLQARVTAVACHPTQDFLAAGYENGTILMVRLTNGAKVVVRREGEASVAALAWNANGSMLAFALQNGDAGLVDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUA9</accession>
    <name>Q3SUA9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0868</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314175.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0868"/>
    <dbReference type="EnsemblBacteria" id="ABA04132">
        <property type="protein sequence ID" value="ABA04132"/>
        <property type="gene ID" value="Nwi_0868"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0868"/>
    <dbReference type="eggNOG" id="ENOG5033QCK">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093433_0_0_5"/>
    <dbReference type="OMA" id="CEPETTA"/>
    <dbReference type="OrthoDB" id="1068088at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="256" mass="28443" checksum="D081948934519903" modified="2005-10-11" version="1">MFKVLDAYSLKARLFPALIAVIPSLAALAILISWSKFGLTSVIATTAIPVLVFAAADIARRLGKHIEERIYAELGGKPSLVMLRSSDTTFDAATKAQYRAFLSLKIAQPVPSEQDERDDPKAVDAFYERAGGWLRENTRDTTKFPVLFNENIIYGFRRNLLGLKWPALTLNAAIVLLCLTLLYRKGIVDTEDDVTVRLLIVLVFAVIHAIYMTFAVGKKSVVDASRTYARQLLLSCETFIGKEKPAKPPIRRRAGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUB6</accession>
    <name>Q3SUB6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0861</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314170.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0861"/>
    <dbReference type="EnsemblBacteria" id="ABA04125">
        <property type="protein sequence ID" value="ABA04125"/>
        <property type="gene ID" value="Nwi_0861"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0861"/>
    <dbReference type="eggNOG" id="COG5639">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_179036_1_1_5"/>
    <dbReference type="OMA" id="MIRRFMA"/>
    <dbReference type="OrthoDB" id="1902903at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018733">
        <property type="entry name" value="DUF2274"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10038">
        <property type="entry name" value="DUF2274"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="75" mass="8257" checksum="0E90E77FDDAD3260" modified="2005-10-11" version="1">MTKLRLSALPDDKPVKITVKLPAAIHRDLVTYAEILARTSGEATPADPAKLVAPMLQRFMATDKGFNKAKRTTQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUD3</accession>
    <name>Q3SUD3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF2285 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0844</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314155.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0844"/>
    <dbReference type="EnsemblBacteria" id="ABA04108">
        <property type="protein sequence ID" value="ABA04108"/>
        <property type="gene ID" value="Nwi_0844"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0844"/>
    <dbReference type="eggNOG" id="ENOG5032Z6E">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155900_0_0_5"/>
    <dbReference type="OMA" id="ARAKWMT"/>
    <dbReference type="OrthoDB" id="2023377at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018754">
        <property type="entry name" value="DUF2285"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10074">
        <property type="entry name" value="DUF2285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2285" evidence="1">
        <location>
            <begin position="10"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="88" mass="10204" checksum="1194D86543BF05A3" modified="2005-10-11" version="1">MTNKSAFKDSPPLTERVNAYDEAHLATYLRLLDAAEEGADWREVVKIVFGLDAEADPQRAKQMHDSHLARARWMTEHGYRHLLQPRMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUD6</accession>
    <name>Q3SUD6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0841</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0841"/>
    <dbReference type="EnsemblBacteria" id="ABA04105">
        <property type="protein sequence ID" value="ABA04105"/>
        <property type="gene ID" value="Nwi_0841"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0841"/>
    <dbReference type="eggNOG" id="ENOG502Z7KV">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1592863_0_0_5"/>
    <dbReference type="OMA" id="MPFINGI"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="167" mass="19074" checksum="EE73360D256688F8" modified="2005-10-11" version="1">MYFRGFYCWNRGFYCWNSEVGSKMLGIASFYLRAVCMNRNLWGCENFEEITIRHSKFAAQRFAHEAAPALTSFAHSSPAPFVAGIKAARERIVARIDDDRETFLRKRGFSKAETAKIIETVLQEEGRPPESIFDFVQGMTAHARTKTHQDTRLELEGKAKTLLEKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUF7</accession>
    <name>Q3SUF7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0819</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314132.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0819"/>
    <dbReference type="EnsemblBacteria" id="ABA04084">
        <property type="protein sequence ID" value="ABA04084"/>
        <property type="gene ID" value="Nwi_0819"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0819"/>
    <dbReference type="eggNOG" id="ENOG50345QN">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1179228_0_0_5"/>
    <dbReference type="OrthoDB" id="2100947at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="235" mass="25094" checksum="89111A98CC278C58" modified="2005-10-11" version="1">MTAALNPMPSADLAKASLDRLDAVASAMERKWGIDRLPKLVDAPLAVRFRSQAERLDEAIRSDISAAISAQAEAMLRAWNALDAAAITGGWKPLAPTIWEAVLPETGEVIAIVRDSDEAFALGHERKGAVWTLAEVAIAIEAFGDTVRATKEAFPGAEVTAVRPAGTTAKLAIGSAAPVVATERETKPARKLPARNRRNRSAGFAGLYAPLLDPRPETPSTKPPIDWERGDDIPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUI3</accession>
    <name>Q3SUI3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0793</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314106.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0793"/>
    <dbReference type="EnsemblBacteria" id="ABA04058">
        <property type="protein sequence ID" value="ABA04058"/>
        <property type="gene ID" value="Nwi_0793"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0793"/>
    <dbReference type="eggNOG" id="ENOG50331F6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2434473_0_0_5"/>
    <dbReference type="OMA" id="VARICPE"/>
    <dbReference type="OrthoDB" id="2057366at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="87" mass="10061" checksum="9938C9894308D57B" modified="2005-10-11" version="1">MFGDFFMRWRARRARWKADAKRLVAINPPTAFHAAQVEVARARSVQDRAEAWHWMKVAAEVARICPEADLDVERSVQAIRAAGFCYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUK7</accession>
    <name>Q3SUK7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cytochrome bo3 quinol oxidase subunit 3</fullName>
            <ecNumber evidence="9">1.10.3.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_0769</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the cytochrome c oxidase subunit 3 family.</text>
    </comment>
    <dbReference type="EC" id="1.10.3.-" evidence="9"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314083.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0769"/>
    <dbReference type="EnsemblBacteria" id="ABA04034">
        <property type="protein sequence ID" value="ABA04034"/>
        <property type="gene ID" value="Nwi_0769"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0769"/>
    <dbReference type="eggNOG" id="COG1845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044071_3_0_5"/>
    <dbReference type="OMA" id="FLDLLWV"/>
    <dbReference type="OrthoDB" id="1502742at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019646">
        <property type="term" value="P:aerobic electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024791">
        <property type="entry name" value="Cyt_c/ubiquinol_Oxase_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000298">
        <property type="entry name" value="Cyt_c_oxidase-like_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035973">
        <property type="entry name" value="Cyt_c_oxidase_su3-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013833">
        <property type="entry name" value="Cyt_c_oxidase_su3_a-hlx"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11403">
        <property type="entry name" value="PTHR11403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00510">
        <property type="entry name" value="COX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81452">
        <property type="entry name" value="SSF81452"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50253">
        <property type="entry name" value="COX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="219"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="COX3" evidence="8">
        <location>
            <begin position="1"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA04034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="241" mass="26148" checksum="22837C128B72E9FF" modified="2005-10-11" version="1">MTDALITADHGDEADAETARRAIEEKAYGFRLCLVSDAIIFALLFAIFIAMTRDAADVSTERPLLRLPRIFPETMLLLASSATLGFASIAAAARQRWRAIIWLGLTFVLGLGFVVTEILELYGMAAAHAGPWQSGFLSAFFTLIGAHGAHVSLGLIWIALLGGELAWRGPALDHDGFGSNRSKTMSVIDSSILERDAGGKPLHTHPAPGAVSRLHRLSLFWHFLVIVWISIFALVYLPGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUL0</accession>
    <name>Q3SUL0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytochrome-c oxidase</fullName>
            <ecNumber evidence="3">7.1.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Cytochrome aa3 subunit 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Cytochrome c oxidase polypeptide III</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_0228</name>
        <name evidence="13" type="ordered locus">Nwi_0766</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="13">Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome c oxidase subunit 3 family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.9" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313563.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0228"/>
    <dbReference type="EnsemblBacteria" id="ABA03496">
        <property type="protein sequence ID" value="ABA03496"/>
        <property type="gene ID" value="Nwi_0228"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04031">
        <property type="protein sequence ID" value="ABA04031"/>
        <property type="gene ID" value="Nwi_0766"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0228"/>
    <dbReference type="KEGG" id="nwi:Nwi_0766"/>
    <dbReference type="eggNOG" id="COG1845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044071_0_0_5"/>
    <dbReference type="OMA" id="ALLWPDF"/>
    <dbReference type="OrthoDB" id="1502742at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019646">
        <property type="term" value="P:aerobic electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01665">
        <property type="entry name" value="Cyt_c_Oxidase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024791">
        <property type="entry name" value="Cyt_c/ubiquinol_Oxase_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033945">
        <property type="entry name" value="Cyt_c_oxase_su3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000298">
        <property type="entry name" value="Cyt_c_oxidase-like_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035973">
        <property type="entry name" value="Cyt_c_oxidase_su3-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013833">
        <property type="entry name" value="Cyt_c_oxidase_su3_a-hlx"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11403">
        <property type="entry name" value="PTHR11403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00510">
        <property type="entry name" value="COX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81452">
        <property type="entry name" value="SSF81452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50253">
        <property type="entry name" value="COX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="204"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="COX3" evidence="11">
        <location>
            <begin position="6"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA03496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA04031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="284" mass="31666" checksum="96C26BEE0B916308" modified="2005-10-11" version="1">MATAQVKHDYHLVDPSPWPIVGATAAFIMAVGAILWMHHMTAAAPIIFGIGVVGVLYTMASWWADVIREAEHKGDHTRVVQISHRYGMILFITSEVMFFVAWFWAFFNSALFPGDPVHVTREALFGGVWPPKGIQTFDPWHLPLLNTLILLTSGTTVTWAHHALLNGDRKGLKWGLILTIALGAIFTCVQAYEYQHAAFSFAGNVYGATFFMATGFHGFHVLVGTIFLIVCLFRANAGHFTPSQHLGFEFAAWYWHFVDVVWLFLFVCIYIWGNGAAAMAHAAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUM2</accession>
    <name>Q3SUM2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Electron transfer flavoprotein alpha and beta-subunits</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0754</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ETF alpha-subunit/FixB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314073.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0754"/>
    <dbReference type="EnsemblBacteria" id="ABA04019">
        <property type="protein sequence ID" value="ABA04019"/>
        <property type="gene ID" value="Nwi_0754"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0754"/>
    <dbReference type="eggNOG" id="COG2025">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034178_0_1_5"/>
    <dbReference type="OMA" id="WRPYAEQ"/>
    <dbReference type="OrthoDB" id="1400253at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01715">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001308">
        <property type="entry name" value="ETF_a/FixB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033947">
        <property type="entry name" value="ETF_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014731">
        <property type="entry name" value="ETF_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018206">
        <property type="entry name" value="ETF_asu_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153">
        <property type="entry name" value="PTHR43153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00766">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000089">
        <property type="entry name" value="Electra_flavoP_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00696">
        <property type="entry name" value="ETF_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ETF" evidence="6">
        <location>
            <begin position="3"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="314" mass="32612" checksum="E10016998C00064C" modified="2005-10-11" version="1">MTTLLIAEHDNASLKDVTGKALTAAIQLGSPVEVLVAGENARGAAESAAKLQGVAKVLLADDPAYAHDLAEPLAALIRSLASGYDALVAPATSRFKNVMPRVAALLDVMQISEIIKVVAADTFERPIYAGNAIQTVRSRDARRVITVRTSTFAAAGEGGNAPVETIAATADPGLSSFVGERVARNNRPELASARIIVSGGRGMRSSANFTKYIEPLADKLGAGVGASRAAVDAGFAPNDWQVGQTGKVVAPDLYIAVGISGAIQHLAGMKDSKVIVAINKDADAPIFQVADYGLVADLYQAVPELTEELGKLDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUV4</accession>
    <name>Q3SUV4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type nitrate/sulfonate/bicarbonate transport system ATPase component</fullName>
            <ecNumber evidence="7">3.6.3.25</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0671</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in beta-(1--&gt;2)glucan export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.25" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0671"/>
    <dbReference type="EnsemblBacteria" id="ABA03937">
        <property type="protein sequence ID" value="ABA03937"/>
        <property type="gene ID" value="Nwi_0671"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0671"/>
    <dbReference type="eggNOG" id="COG1116">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_5"/>
    <dbReference type="OMA" id="IMITHNR"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="22"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="58"/>
            <end position="65"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="279" mass="30908" checksum="2320DF30E991E8FE" modified="2005-10-11" version="1">MNSTLAQPAETGIRRPAQTGHIILDRVTVRFGEDVRGRRAIFDTSLEIRPREFVCLLGPSGCGKSTLLNCIAGYIRPSSGEVRVDGHIVERPGPERGMVFQQYSLFPWKSIRDNIAFGPLRAGKSRAEALAIADHFLTMIGLSGFSKRYPAELSGGMQQRVGIARALANYPSVLLMDEPFGALDAQTRAMMQESLLRIWGEVETTVVFVTHDVDEAIFLADRIVVMSAGPGRIIADQDVPLLRPRSPDILTDDTFIRIKKSCLARIRAESLKAFQQQNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUV9</accession>
    <name>Q3SUV9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Amino acid/amide ABC transporter membrane protein 2, HAAT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0666</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313988.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0666"/>
    <dbReference type="EnsemblBacteria" id="ABA03932">
        <property type="protein sequence ID" value="ABA03932"/>
        <property type="gene ID" value="Nwi_0666"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0666"/>
    <dbReference type="eggNOG" id="COG4177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031365_0_1_5"/>
    <dbReference type="OMA" id="GHTGINT"/>
    <dbReference type="OrthoDB" id="1456257at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06581">
        <property type="entry name" value="TM_PBP1_LivM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043428">
        <property type="entry name" value="LivM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30482">
        <property type="entry name" value="PTHR30482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="130"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="219"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="265"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="349" mass="36642" checksum="20C495A518F68E88" modified="2005-10-11" version="1">MSDAASTSTTEVVGRSFALSSLITSAVLIALVTLPAVLPEFWTVNIFVRAMIFGIVALSLTFLAHYGGFISLAQMVPAGVAGYTIAVTVPDAIPANGFQLPYGPAVALALVLSTVAGLTIGAVAVRTREIYLLMITLAIAVSFYFFVQANVEFLNGYEGIRNVMGPDILSVPLRTPAIFYHAVLAVSVALYALVIYVSRAPLGLVLQGIRDNPRRISALGFNVALHRIVAFGLAGFIAGVGGVLLTFYNIGISPGTVSTHATISILIIAVIGGLGHPIGAFIGAFIFTIFDTFAADIYDRDRFNTLIGAVFLTIVVASPDGIIGLIKRGNALLARDRSPVSRSQTSIAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUW2</accession>
    <name>Q3SUW2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0663</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03929">
        <property type="protein sequence ID" value="ABA03929"/>
        <property type="gene ID" value="Nwi_0663"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0663"/>
    <dbReference type="HOGENOM" id="CLU_1794478_0_0_5"/>
    <dbReference type="OMA" id="THIIAIP"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="144" mass="16051" checksum="A6AA8C67283B33D3" modified="2005-10-11" version="1">MFYGPHSSSLKQNKGRTSLPLSELNRLVRYRQAAFACMTHIIAIPPQHIIIGMPLFAMFIMFWQHCMNMSFIDASMGFISQVMPLAVIVQVIWHIMVGIDIMPPIGIMPFIIMGDVMGDAPPIMGIIICIAGVMCLSLQEVNDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUY1</accession>
    <name>Q3SUY1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Short-chain dehydrogenase/reductase</fullName>
            <ecNumber evidence="3">5.1.3.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0643</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the polysaccharide synthase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.2" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313970.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0643"/>
    <dbReference type="EnsemblBacteria" id="ABA03910">
        <property type="protein sequence ID" value="ABA03910"/>
        <property type="gene ID" value="Nwi_0643"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0643"/>
    <dbReference type="eggNOG" id="COG1086">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013560_4_1_5"/>
    <dbReference type="OMA" id="HEVMIPK"/>
    <dbReference type="OrthoDB" id="662797at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003978">
        <property type="term" value="F:UDP-glucose 4-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd05237">
        <property type="entry name" value="UDP_invert_4-6DH_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003869">
        <property type="entry name" value="Polysac_CapD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020025">
        <property type="entry name" value="PseB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02719">
        <property type="entry name" value="Polysacc_synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03589">
        <property type="entry name" value="PseB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polysacc_synt_2" evidence="2">
        <location>
            <begin position="7"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="333" mass="37193" checksum="EF13425A1023F3C4" modified="2005-10-11" version="1">MFNGKSVFVSGGTGSFGRKFASILLERYDLARLVFYSRDELKQFEMQQEVNAPCIRWLIGDVRDGARLIEATRGIDCIVHAAAMKQVTASEYNPTECIRTNIHGAENIVAAALANNVEQVIALSSDKAANPINLYGATKLASDKLFIAANNMAGRRRTAFSVVRYGNVAGSRGSVVPFFEKLMAQGATSLPITDTAMTRFWISLQESVDFVLRCFQRMQGGEIFVPKLPSIRIVDLATAMAPHLPQNLIGIRPGEKLHEILCPADNSHLTLEFSDHYVMRPSIKFHDRGCDYLENRLRETGAPVTRGFEYNSGTNPVRYSVEQIREFYERTRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV31</accession>
    <name>Q3SV31_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lectin-like protein BA14k</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_0593</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Has immunoglobulin-binding and hemagglutination properties, and can bind to mannose. Essential for virulence. May be involved in LPS biosynthesis or polysaccharide transport.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the BA14k family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313921.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0593"/>
    <dbReference type="EnsemblBacteria" id="ABA03860">
        <property type="protein sequence ID" value="ABA03860"/>
        <property type="gene ID" value="Nwi_0593"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0593"/>
    <dbReference type="eggNOG" id="ENOG5033457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095992_3_0_5"/>
    <dbReference type="OMA" id="FRSYDAR"/>
    <dbReference type="OrthoDB" id="2188014at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012413">
        <property type="entry name" value="BA14K"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07886">
        <property type="entry name" value="BA14K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0430">Lectin</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0843">Virulence</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229334" description="Lectin-like protein BA14k" evidence="8">
        <location>
            <begin position="27"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA03860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="127" mass="12899" checksum="DDC969FD1739412E" modified="2005-10-11" version="1">MMRSKILLTALFAGSVALTFSVPATAAPLMAQSGVSTAGPSSIETVQYYRRGYYGPGYYGRGYGGGAGALIGGLAAGAIVGGAIAAGQANAAAAAQQNMAYCAQRFRSYDPRSGTYLGTDGYRHPCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="129" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV34</accession>
    <name>Q3SV34_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Sulfite reductase [NADPH] flavoprotein alpha-component</fullName>
            <shortName evidence="12">SiR-FP</shortName>
            <ecNumber evidence="12">1.8.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">Nwi_0590</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate. The flavoprotein component catalyzes the electron flow from NADPH -&gt; FAD -&gt; FMN to the hemoprotein component.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>3 H2O + hydrogen sulfide + 3 NADP(+) = 4 H(+) + 3 NADPH + sulfite</text>
            <dbReference type="Rhea" id="RHEA:13801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17359"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12 13">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="12 13">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12 13">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="12 13">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12">Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Alpha(8)-beta(8). The alpha component is a flavoprotein, the beta component is a hemoprotein.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.2" evidence="12"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313918.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0590"/>
    <dbReference type="EnsemblBacteria" id="ABA03857">
        <property type="protein sequence ID" value="ABA03857"/>
        <property type="gene ID" value="Nwi_0590"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0590"/>
    <dbReference type="eggNOG" id="COG0369">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_17_7_5"/>
    <dbReference type="OMA" id="QKRYQRD"/>
    <dbReference type="OrthoDB" id="707164at2"/>
    <dbReference type="UniPathway" id="UPA00140">
        <property type="reaction ID" value="UER00207"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004783">
        <property type="term" value="F:sulfite reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019344">
        <property type="term" value="P:cysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070814">
        <property type="term" value="P:hydrogen sulfide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.990.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010199">
        <property type="entry name" value="CysJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003097">
        <property type="entry name" value="CysJ-like_FAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001094">
        <property type="entry name" value="Flavdoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023173">
        <property type="entry name" value="NADPH_Cyt_P450_Rdtase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00667">
        <property type="entry name" value="FAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000207">
        <property type="entry name" value="SiR-FP_CysJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00369">
        <property type="entry name" value="FLAVODOXIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="SSF52343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01931">
        <property type="entry name" value="cysJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11 12" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="8 12" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6 12 13" id="KW-0274">FAD</keyword>
    <keyword evidence="3 12" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4 12 13" id="KW-0288">FMN</keyword>
    <keyword evidence="10" id="KW-0520">NAD</keyword>
    <keyword evidence="7 12 13" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 12 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 12" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="15">
        <location>
            <begin position="74"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="16">
        <location>
            <begin position="246"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="223"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="80"/>
            <end position="85"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="127"/>
            <end position="130"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="163"/>
            <end position="172"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="401"/>
            <end position="404"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="419"/>
            <end position="421"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="425"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="434"/>
            <end position="437"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="533"/>
            <end position="534"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="539"/>
            <end position="543"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="575"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="613"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR000207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000207-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ABA03857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="613" mass="66301" checksum="ABE0D52FB35B19E0" modified="2005-10-11" version="1">MTKLEFPGSALTTDQWTQVNQLATSLSHEQAIWISGYFAGLSHRARAGAAATEIVELAPPSQVGADGAAATRLLTVLFASETGNSKTLAKALVEKAAAQGIQARLADMAEYKTRGLKDEQDLLVITSTHGEGDAPQTAVGFFEFLESRKAPKLPQLRYAVLALGDSTYEKYCEAGKRVDRRLEELGAQRLADRVDCDVDYEDAADAWIASVVTKLAPAAQAPASAPGASAATMRSGAPSPATFDKKNPFQAAVIDNIVLTGRGSTKETRHIELSLADSGLTYQPGDALGVVPRNDPALVEAILEKLSLSADAPVTVKQGTISLGEALSGTFEITALTPRFLDLWSELTGSDELQALRAPDQKEARAAFQHNYHILDVIGRFPAPGIDAARLVAGLRPLQPRLYSIASSLAAAPDEAHLTVSTVRYTLHDLPRTGVASGYLAARTEADATVPVYIQSNDHFHLPDDDVPILMIGAGTGVAPYRAFMQEREASGASGRSWLFFGERNFRSDFLYQVEWQELLKNGVLSRLDLAFSRDAAPKTYVQDRLRRQGRDVYAWLEEGAYVYVCGDSAHMAPDVHAALTEIVAEHGGLDREEAGAYLSALQRDRRYRLDVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVC9</accession>
    <name>Q3SVC9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Farnesyl-diphosphate synthase</fullName>
            <ecNumber evidence="3">2.5.1.10</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0495</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.10" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313823.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0495"/>
    <dbReference type="EnsemblBacteria" id="ABA03762">
        <property type="protein sequence ID" value="ABA03762"/>
        <property type="gene ID" value="Nwi_0495"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0495"/>
    <dbReference type="eggNOG" id="COG0142">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014015_0_0_5"/>
    <dbReference type="OMA" id="CEGQALD"/>
    <dbReference type="OrthoDB" id="1861068at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004337">
        <property type="term" value="F:geranyltranstransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="SSF48576"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="308" mass="32478" checksum="57C6B2FD5611D0BB" modified="2005-10-11" version="1">MTSGLSSADFAIRLDTTADETEAVLEKLLSDAPLPDEIARPRRLTDAMRYSSLGGGKRLRPFLVVESSAVFGVPREAALLAGAALECIHCYSLIHDDLPAMDNSDLRRGRPTLHKTTDDATAILAGDGLLTLAFDIITRDEVHDDPAVRLLLTRALARAAGIGGMVGGQLLDLAGEGRFGDRGPVDVARVQQMKTGALLRFGCIAGALLGQASQKDYQALDAYGRALGEAFQIADDLLDVESDAAALGKPTGADAALGKTTFVTQLGIDGAKKRVRDLLAKADEALSVFGSRGDVLRAAAHFVANRKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVH0</accession>
    <name>Q3SVH0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_0454</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0410 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313785.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0454"/>
    <dbReference type="EnsemblBacteria" id="ABA03721">
        <property type="protein sequence ID" value="ABA03721"/>
        <property type="gene ID" value="Nwi_0454"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0454"/>
    <dbReference type="eggNOG" id="COG2261">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_160040_2_0_5"/>
    <dbReference type="OMA" id="GWIMTIV"/>
    <dbReference type="OrthoDB" id="2084822at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007341">
        <property type="entry name" value="Transgly_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33884">
        <property type="entry name" value="PTHR33884"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04226">
        <property type="entry name" value="Transgly_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA03721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="90" mass="9269" checksum="61AB54E92AF384A0" modified="2005-10-11" version="1">MSGILWIILVGFVAGVIAKFLSPGPNNPSGFILTTVLGIAGAFLATFIGQQIGHYSPDQGAGFITSAIGAVVVLFIWNRLVARGAISGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVJ7</accession>
    <name>Q3SVJ7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Primosomal protein N'</fullName>
            <ecNumber evidence="9">3.6.4.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ATP-dependent helicase PriA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">priA</name>
        <name evidence="11" type="ordered locus">Nwi_0427</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in the restart of stalled replication forks. Recognizes and binds the arrested nascent DNA chain at stalled replication forks. It can open the DNA duplex, via its helicase activity, and promote assembly of the primosome and loading of the major replicative helicase DnaB onto DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the primosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the helicase family. PriA subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="9"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313758.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0427"/>
    <dbReference type="EnsemblBacteria" id="ABA03694">
        <property type="protein sequence ID" value="ABA03694"/>
        <property type="gene ID" value="Nwi_0427"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0427"/>
    <dbReference type="eggNOG" id="COG1198">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013353_4_0_5"/>
    <dbReference type="OMA" id="RCHYCGY"/>
    <dbReference type="OrthoDB" id="1132322at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006268">
        <property type="term" value="P:DNA unwinding involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1440.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00983">
        <property type="entry name" value="PriA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005259">
        <property type="entry name" value="PriA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041222">
        <property type="entry name" value="PriA_3primeBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042115">
        <property type="entry name" value="PriA_3primeBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041236">
        <property type="entry name" value="PriA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040498">
        <property type="entry name" value="PriA_CRR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17764">
        <property type="entry name" value="PriA_3primeBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18074">
        <property type="entry name" value="PriA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18319">
        <property type="entry name" value="PriA_CRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00595">
        <property type="entry name" value="priA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 9" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 11" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1 9" id="KW-0639">Primosome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="219"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="9">
        <location>
            <begin position="446"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="9">
        <location>
            <begin position="473"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA03694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="738" mass="81059" checksum="5C9AC68C3D612847" modified="2005-10-11" version="1">MDRSSRHGTIPASSSAPRVVDVLVPVAIDHAYSYRVPRGMEVRPGEVVTVPLGPREVVAVVWAGNPAPDPRLHNRLKDISGKLDVPPLRDELRTLIDWVANYTLSPRGMVMRMSLRMGEHLGPERVRPGVRLSGEPPRRMTPARRRLLAVLSDGLLRAKSDAAREAGVSAGVVDGLVDEGTLTTEPMPRSPPPPPPDPDFAVPDFSRSQRAAVDAMRALASNGTFHVALLDGVTGSGKTEVYFEAVAETVRRGRQVLILMPEIALTGQFRDRFAARFGERPLEWHSELTPRNRARNWAAIAAGEAQVVVGARSALFLPYADLGLIIIDEEHDQAYKQDEGVRYHARDMAVVRAHVAKIPIILASATPSVESEVNARKGRYQRIALTSRFGGQHLPRIELIDLRREGPSRGRFISPRLAGEIVTAIERREQALLFLNRRGYAPLTLCRGCGHRFACTICDAWLVDHRFRDRLVCHHCGFSMPRPHQCPHCAAEQSLVAVGPGVERLQEEAAALFPDARTMVLSSDLITSIETMRSELNDIAEGRVDIIIGTQLVAKGHHFPRLNLVGVVDADLGLGNGDPRAAERTFQLLNQVIGRTGREQGRGVGYLQTHQPEHPVMKALVAGDREAFYASEIAARERTGYPPFGRLASLIISAGDRPSAESFARQLAAVAPVDERIQVLGPAEAPLALIKGRYRFRLLVKSARGVDLSGYLRDWLASAPKTRGNLKLEVDVDPQSFL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVM8</accession>
    <name>AROB_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-dehydroquinate synthase</fullName>
            <shortName evidence="1">DHQS</shortName>
            <ecNumber evidence="1">4.2.3.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aroB</name>
        <name type="ordered locus">Nwi_0396</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) to dehydroquinate (DHQ).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate = 3-dehydroquinate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21968"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="EC" id="4.2.3.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 divalent metal cation per subunit. Can use either Co(2+) or Zn(2+).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 2/7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the sugar phosphate cyclases superfamily. Dehydroquinate synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.4" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313727.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVM8"/>
    <dbReference type="SMR" id="Q3SVM8"/>
    <dbReference type="STRING" id="323098.Nwi_0396"/>
    <dbReference type="EnsemblBacteria" id="ABA03663">
        <property type="protein sequence ID" value="ABA03663"/>
        <property type="gene ID" value="Nwi_0396"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0396"/>
    <dbReference type="eggNOG" id="COG0337">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001201_0_2_5"/>
    <dbReference type="OMA" id="YGVIWDA"/>
    <dbReference type="OrthoDB" id="1677032at2"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00085"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003856">
        <property type="term" value="F:3-dehydroquinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00110">
        <property type="entry name" value="DHQ_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016037">
        <property type="entry name" value="DHQ_synth_AroB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030963">
        <property type="entry name" value="DHQ_synth_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030960">
        <property type="entry name" value="DHQS/DOIS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01761">
        <property type="entry name" value="DHQ_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001455">
        <property type="entry name" value="DHQ_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01357">
        <property type="entry name" value="aroB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0170">Cobalt</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000231101" description="3-dehydroquinate synthase">
        <location>
            <begin position="1"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="81"/>
            <end position="86"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="115"/>
            <end position="119"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="139"/>
            <end position="140"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00110"/>
        </source>
    </evidence>
    <sequence length="383" mass="40415" checksum="6738F35324BB64CC" modified="2005-10-11" version="1">MTIPIKESDPITVDVALGERSYDIVIGRGVLPSLGERIAALRPGARVAVVTDARVATHWLQRTEASLTGAGLTTSRIVVDEGEVSKSYAGVEFVCEELIKARIERNDLVVALGGGVVGDLAGFAAAIVRRGVDFVQVPTSLLAQVDSSVGGKTGINSPQGKNLVGAFHQPILVVADTAVLDTLSPRQFRAGYAEVAKYGLIGDEAFFAWLEVHHADIVKGDAAREHAVATSCRAKAAIVARDERESGERALLNLGHTFGHALEAATGFCGRLYHGEGVSIGMVLAAELSAQLGMIAAADVSRIERHLAAAGLPTRLQDIAGFRQEGLADADALMTLMAQDKKVRRGRLTFILLKAIGQAVVSSDVEPSMVRDFLARKLADAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVQ0</accession>
    <name>Q3SVQ0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransf_11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0374</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313705.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0374"/>
    <dbReference type="EnsemblBacteria" id="ABA03641">
        <property type="protein sequence ID" value="ABA03641"/>
        <property type="gene ID" value="Nwi_0374"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0374"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048277_0_0_5"/>
    <dbReference type="OMA" id="WARMEHT"/>
    <dbReference type="OrthoDB" id="1730305at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="1">
        <location>
            <begin position="86"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="246" mass="27600" checksum="C17D03123C9714CE" modified="2005-10-11" version="1">MAIDVIDLRDFYSQRLGVVARRLISRAIHAHWPNMKGQCVAGLGYPTPYLGLFREECERCIALMPAAQGVLKWPTARPALATLVDEFSLPLRDAVADRILLVHALEMSDDPESLLREVWRVLSPSGRMMVIVPNRRGIWTRTDVTPFGHGQPYSRSQITQLLRQTWFTPSAWGEALFVPPINGGWFLRSAAVWERIGAALSLPFAGVHLVEATKQVYRPIAAKREHSRLIPVLQPTLAPSSYNAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="130" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVR7</accession>
    <name>Q3SVR7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">DNA polymerase I</fullName>
            <ecNumber evidence="3 13">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">polA</name>
        <name evidence="17" type="ordered locus">Nwi_0357</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">In addition to polymerase activity, this DNA polymerase exhibits 5'-3' exonuclease activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Single-chain monomer with multiple functions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 13">Belongs to the DNA polymerase type-A family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="3 13"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313689.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0357"/>
    <dbReference type="EnsemblBacteria" id="ABA03624">
        <property type="protein sequence ID" value="ABA03624"/>
        <property type="gene ID" value="Nwi_0357"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0357"/>
    <dbReference type="eggNOG" id="COG0258">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0749">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004675_0_0_5"/>
    <dbReference type="OMA" id="NRPPMPD"/>
    <dbReference type="OrthoDB" id="1220182at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09898">
        <property type="entry name" value="H3TH_53EXO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020046">
        <property type="entry name" value="5-3_exonucl_a-hlix_arch_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002421">
        <property type="entry name" value="5-3_exonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019760">
        <property type="entry name" value="DNA-dir_DNA_pol_A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001098">
        <property type="entry name" value="DNA-dir_DNA_pol_A_palm_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020045">
        <property type="entry name" value="DNA_polI_H3TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018320">
        <property type="entry name" value="DNA_polymerase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002298">
        <property type="entry name" value="DNA_polymerase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10133">
        <property type="entry name" value="PTHR10133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01367">
        <property type="entry name" value="5_3_exonuc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02739">
        <property type="entry name" value="5_3_exonuc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00476">
        <property type="entry name" value="DNA_pol_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00868">
        <property type="entry name" value="DNAPOLI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00474">
        <property type="entry name" value="35EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00475">
        <property type="entry name" value="53EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00482">
        <property type="entry name" value="POLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="SSF47807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00593">
        <property type="entry name" value="pola"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00447">
        <property type="entry name" value="DNA_POLYMERASE_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0227">DNA damage</keyword>
    <keyword evidence="13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="13" id="KW-0235">DNA replication</keyword>
    <keyword evidence="11 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 13" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="9" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6 13 17" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13 17" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="53EXOc" evidence="15">
        <location>
            <begin position="31"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="3'-5' exonuclease" evidence="14">
        <location>
            <begin position="399"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="domain" description="POLAc" evidence="16">
        <location>
            <begin position="764"/>
            <end position="970"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ABA03624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1010" mass="110667" checksum="3BFC0278CFC19C05" modified="2005-10-11" version="1">MSKKPAAAEIPAIIPATIPPVKAAGTTLQGKHVFLVDGSSYIFRAYHALPPLNRKSDGLQVNAVLGFCNMLWKLLRDMPKNDRPTHLAIIFDKSEVTFRNKLYPAYKAHRPPAPDDLIPQFALIREAVKAFDLPCLEQIGFEADDLIATYVRQACERGATATIVSSDKDLMQLVNGCVTMYDTMKDRRLGIAEVIEKFGVPPEKVVEVQALAGDSVDNVPGVPGIGVKTAAQLITEYGDLDTLLARATEIKQPKRREALIENAEKARISRQLVLLDDHVALDVPLDDLAVQEPDARKLIAFLKAMEFTTLTKRVADYSEVNAAEIEPDAKHTSGRSAMVAKASATALTGDLFGSSDSVAKGTPAGTAKATGDTKMKTPAALAAARLETVRALPVRPDAYETIRTLDRLRDWIARIDDNGSFAFEALAPTIDPMQTELSGIALALAPNHACYIPLSHKQSGDSAGLFAAGLAPDQIAIRDALELLKPILESRGHLKIGLNVKFSAVLLAQHGIVMQNHDDIELISYALDAGRGSHDLASLAKRWLGHATVSHGELTGSGRNRLAFDQVAIDRATAYAAEYADMTLRLWQVLKPRLVAERMNTVYETLERPMIATLAQMERRGITIDRQVLSRLSGEFAQSAARLEAEIQQLAGEPINVGSPKQIGEIMFGKMGLPGGSKTKTGAWSTSAQILDELAEQGHDFPRKILDWRQVSKLKSTYTDALPEYVHPQTRRVHTTYALAATTTGRLSSNEPNLQNIPVRNEEGRKIRRAFIAAPGHKLVSADYSQIELRLLAEIADIPVLKQAFRDGLDIHAMTASEMFGVPVQGMPGEIRRRAKAINFGIIYGISAFGLANQLGIPREEAAAYIKKYFERFPGIRAYMDATREFCREHGYVTTLFGRKCHYPDIKSPNPSHRAFNERAAINARLQGSAADIIRRAMARMDDALAARNLSARMLLQVHDELIFEVPDDEVAATLPVVQHVMQDAPFPAVLLSVPLQVDARAADNWDEAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVS2</accession>
    <name>Q3SVS2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Arginase</fullName>
            <ecNumber evidence="3 10">3.5.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_0352</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>H2O + L-arginine = L-ornithine + urea</text>
            <dbReference type="Rhea" id="RHEA:20569"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16199"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="EC" id="3.5.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="10">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the arginase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.3.1" evidence="3 10"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0352"/>
    <dbReference type="EnsemblBacteria" id="ABA03619">
        <property type="protein sequence ID" value="ABA03619"/>
        <property type="gene ID" value="Nwi_0352"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0352"/>
    <dbReference type="eggNOG" id="COG0010">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039478_6_2_5"/>
    <dbReference type="OMA" id="IATCFGQ"/>
    <dbReference type="UniPathway" id="UPA00158">
        <property type="reaction ID" value="UER00270"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004053">
        <property type="term" value="F:arginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006525">
        <property type="term" value="P:arginine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000050">
        <property type="term" value="P:urea cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd09989">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014033">
        <property type="entry name" value="Arginase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020855">
        <property type="entry name" value="Ureohydrolase_Mn_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43782">
        <property type="entry name" value="PTHR43782"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036979">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00116">
        <property type="entry name" value="ARGINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="SSF52768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01229">
        <property type="entry name" value="rocF_arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01053">
        <property type="entry name" value="ARGINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0056">Arginine metabolism</keyword>
    <keyword evidence="7 9 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="305"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="305"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA03619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="331" mass="35565" checksum="907A4E6A8E93A2E5" modified="2005-10-11" version="1">MTDRRKVALLGVPIDMGASQRGTLMGPAALRTAGITPVLESLGIDVLDHGDIALAGVAPATDAIPAKANNYRDIQTWIRALSPRAYDLARSGAIPIFLGGDHSLSMGSINGVARYWHEQGRELFVLWLDAHADYNTPLTTMTANMHGMSAAFVCGESGLDGLLGDEPRASIPPAHLDLFGIHSIDPLEKKLVQDRRIAVADMRAIDEFGVAVLIHRVIERVRARNGVLHLSFDLDVLDPGIAPGVGTTVPGGATYREAHLVMELLYDSGLVGSVDIVELNPFLDERGRTARVAVELLASLFGQQITDRPTPSNTLSEPRPAQVAKIRPTRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVU4</accession>
    <name>Q3SVU4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Creatininase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0330</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the creatininase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313662.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0330"/>
    <dbReference type="EnsemblBacteria" id="ABA03597">
        <property type="protein sequence ID" value="ABA03597"/>
        <property type="gene ID" value="Nwi_0330"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0330"/>
    <dbReference type="eggNOG" id="COG1402">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055029_0_0_5"/>
    <dbReference type="OMA" id="PCFLWSG"/>
    <dbReference type="OrthoDB" id="984747at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024087">
        <property type="entry name" value="Creatininase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003785">
        <property type="entry name" value="Creatininase/forma_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35005">
        <property type="entry name" value="PTHR35005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02633">
        <property type="entry name" value="Creatininase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102215">
        <property type="entry name" value="SSF102215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="272" mass="29316" checksum="13143C6061444071" modified="2005-10-11" version="1">MIPTPPFDWLAIDWPGVAPEAAANWIAVLPLAATEQHGPHLPLTTDVLIAEAYLAKVREWLPQEIPATFLPVQPIGISTEHMDFPGTRTLSNEAALRAWTALGEEAARRGIRKIVMVSSHGGNSAAMSMVAQDLRARHRMLAVTTAWARFGVPDGLFDAQELRHGVHGGAVETSIMLARYPDRVRSDRIADFSPASIAIEKTYRWLGTQRPAPFAWQAQDLHPSGAAGDATQASAEKGERLLAHGARAFCELLADVARFDPAALTGGPGGRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVV3</accession>
    <name>Q3SVV3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">Heme exporter protein B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Nwi_0321</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 12">Required for the export of heme to the periplasm for the biogenesis of c-type cytochromes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell inner membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the CcmB/CycW/HelB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313653.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0321"/>
    <dbReference type="EnsemblBacteria" id="ABA03588">
        <property type="protein sequence ID" value="ABA03588"/>
        <property type="gene ID" value="Nwi_0321"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0321"/>
    <dbReference type="eggNOG" id="COG2386">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079069_1_0_5"/>
    <dbReference type="OMA" id="VKCLAHW"/>
    <dbReference type="OrthoDB" id="1886257at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015232">
        <property type="term" value="F:heme transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003544">
        <property type="entry name" value="Cyt_c_biogenesis_CcmB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026031">
        <property type="entry name" value="Cyt_c_CcmB_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30070">
        <property type="entry name" value="PTHR30070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03379">
        <property type="entry name" value="CcmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002764">
        <property type="entry name" value="CcmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01190">
        <property type="entry name" value="ccmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="7 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="11 12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="92"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="126"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR002764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABA03588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="222" mass="22449" checksum="3D99A0D033F64806" modified="2005-10-11" version="1">MTALAAVIRRDVRVALRVGGGALIGVLFFLTVVVLMPFAVGPDLALLKKLGPAILWLGALLASLLTLDRLFTADHDDGSLDLIVMGRTPLELVCAAKALAHWLAVGLPLVAATPVLGLLLNLDAVATGAVALTLLAGTPALTFTGMVGAALAVVLRRGGLLLSVLVLPLSIPVLIFGVAASNAAMSGPSPFGTPFSILCALSLASLVIGPFAAASSLRHGLD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW78</accession>
    <name>GRPE_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein GrpE</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">HSP-70 cofactor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">grpE</name>
        <name type="ordered locus">Nwi_0195</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GrpE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313531.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SW78"/>
    <dbReference type="SMR" id="Q3SW78"/>
    <dbReference type="STRING" id="323098.Nwi_0195"/>
    <dbReference type="EnsemblBacteria" id="ABA03463">
        <property type="protein sequence ID" value="ABA03463"/>
        <property type="gene ID" value="Nwi_0195"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0195"/>
    <dbReference type="eggNOG" id="COG0576">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057217_6_2_5"/>
    <dbReference type="OMA" id="YAYEKIA"/>
    <dbReference type="OrthoDB" id="1906715at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00446">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.22.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01151">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000740">
        <property type="entry name" value="GrpE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013805">
        <property type="entry name" value="GrpE_coiled_coil"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009012">
        <property type="entry name" value="GrpE_head"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21237">
        <property type="entry name" value="PTHR21237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01025">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00773">
        <property type="entry name" value="GRPEPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51064">
        <property type="entry name" value="SSF51064"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58014">
        <property type="entry name" value="SSF58014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01071">
        <property type="entry name" value="GRPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0346">Stress response</keyword>
    <feature type="chain" id="PRO_1000137590" description="Protein GrpE">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="197" mass="21108" checksum="2E81354FDF590070" modified="2005-10-11" version="1">MTDSDGKTDKSGEPAAEVEPVVSKPYVMPDDPEDDALDALNKQLAEAKDRTLRTLAEMENLRKRTAREVSDARTYGISGFARDVLEIADNLQRALDAVPADARAAPDPGLKALIEGVELTERSLHNALEKHGVKKFDPAGEKFDPNVHQAMYEVPDPSIPVGTVAQVIQAGYMIGERVLRPALVGVAKGGAKAAAPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWC3</accession>
    <name>Q3SWC3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Amino acid ABC transporter substrate-binding protein, PAAT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0150</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313487.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0150"/>
    <dbReference type="EnsemblBacteria" id="ABA03418">
        <property type="protein sequence ID" value="ABA03418"/>
        <property type="gene ID" value="Nwi_0150"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0150"/>
    <dbReference type="eggNOG" id="COG0834">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019602_18_0_5"/>
    <dbReference type="OMA" id="DTPFENF"/>
    <dbReference type="OrthoDB" id="1632224at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00497">
        <property type="entry name" value="SBP_bac_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="PBPb" evidence="3">
        <location>
            <begin position="76"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="306" mass="34376" checksum="F2AD23A725ED93A5" modified="2005-10-11" version="1">MQPQITVSANERLIRRGAATLGCLLAAGWMLGAWATLDRARAQTTVETATAAPQAVPGFWDPRHRPDRPDLSRLTVIRFLTETDYPPFNYTGPDGNPAGFNVDLARTLCEEIKVTCTIQMRRFETLVDALSSNRGDALIASLAVTPELRKRIDFTDPYYRTPARFVSRRDAAMAEVRPEYLEGKKVGVVAGSAHEAYLKVFFTDAELHGYPNDETLRQALRQNEVDFIFGDAISLAFWINGTDSESCCAFSGGPFIESRYFGEGIGIAVRKGNDVLRQALNWALFRIWERGRYTDLWLRYFSVSPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWD6</accession>
    <name>Q3SWD6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0137</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313474.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0137"/>
    <dbReference type="EnsemblBacteria" id="ABA03405">
        <property type="protein sequence ID" value="ABA03405"/>
        <property type="gene ID" value="Nwi_0137"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0137"/>
    <dbReference type="eggNOG" id="COG2755">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065317_1_1_5"/>
    <dbReference type="OMA" id="HATRAIT"/>
    <dbReference type="OrthoDB" id="1247435at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229278" evidence="1">
        <location>
            <begin position="20"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="253" mass="27048" checksum="D59AF7C77C781C5D" modified="2005-10-11" version="1">MRTGLASALIVLAASSAGVSGCSDQTKNCDVPAYLLTSDSTLSRVASVVKAGGPLNILVVGSRSSTISGAEGEAYPVKLQAALREKLPGIPVNVSVELTPRKTAGDVAPGLGKLVAATRSTLVVWQTGIVDAIRSVDIDEFREAIDEGVTVLRNAGADVVLMNMQYSPRTETMVSMNAYIDNMRLVAQQRDVPVFDRFTIMTQWNERGEFDLFSPVHGASLAKRVHSCLGRALSTFLINTARINSAEPEARRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWD7</accession>
    <name>Q3SWD7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acyltransferase 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0136</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313473.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0136"/>
    <dbReference type="EnsemblBacteria" id="ABA03404">
        <property type="protein sequence ID" value="ABA03404"/>
        <property type="gene ID" value="Nwi_0136"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0136"/>
    <dbReference type="eggNOG" id="COG4645">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041000_2_0_5"/>
    <dbReference type="OMA" id="LEIFCLG"/>
    <dbReference type="OrthoDB" id="717806at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014550">
        <property type="entry name" value="UCP028704_OpgC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38592">
        <property type="entry name" value="PTHR38592"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10129">
        <property type="entry name" value="OpgC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028704">
        <property type="entry name" value="UPC028704"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="341"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="366"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="415" mass="46170" checksum="6D8C6C4893C125B2" modified="2005-10-11" version="1">MPPVIDQVVGVRTKASPASVKRDAPVVLIPTPPRERELRLDLLRGFALWLIFIDHLPQNLLTWLTIRNYGFSDATEIFIFISGYTAALVYGRAMRERGFIVAAARILRRVWQIYVAHIVLFTIFLAEISYVASSSENPLYTEEMGILDFLKQPDVTIVQALLLKFRPVNMDVLPLYVALMLFFPLVLLVMQRSAGLALALSAGLYGLTWCFGLALPAYPNGVWFFNPFAWQLLFVFGAWCALGGAKRMSRILSSPATLSICAAYLLFAFYTTLTWHVPRLNHFMPHIAEQWMYPIDKTNLDVLRFMHFVALAALTVRFLPKHWPGLASPWLRPLILCGQHSLQIFCIGVFLAFAGQFILAEYSGGAVVHFLISVCGILIMCAAAWVFSWYKGVAGKGGSRAKSADGDADLAGGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWG6</accession>
    <name>Q3SWG6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tim44 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_0107</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Tim44 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313444.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0107"/>
    <dbReference type="EnsemblBacteria" id="ABA03375">
        <property type="protein sequence ID" value="ABA03375"/>
        <property type="gene ID" value="Nwi_0107"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0107"/>
    <dbReference type="eggNOG" id="COG4395">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086329_1_1_5"/>
    <dbReference type="OMA" id="WKLVGTG"/>
    <dbReference type="OrthoDB" id="1536134at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039544">
        <property type="entry name" value="Tim44-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007379">
        <property type="entry name" value="Tim44-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016985">
        <property type="entry name" value="UCP031890_Tim44-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10721">
        <property type="entry name" value="PTHR10721"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04280">
        <property type="entry name" value="Tim44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031890">
        <property type="entry name" value="UCP031890_transporter_Tim44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00978">
        <property type="entry name" value="Tim44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tim44" evidence="5">
        <location>
            <begin position="86"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA03375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="234" mass="25411" checksum="5AF7D031CAA15862" modified="2005-10-11" version="1">MDIYTIIFLALAVFIFLRLRSVLGQRTGSERPPYDRATREVLGGTQDNNVVPVPGTVINQSSAAPSADVGGAADRWKSVAEPGTPLALGLDAIAAQDSSFDPAHFLSGARSAYEMIVLAFANGDRRTLKDLLSSDVYESFDAAIKDRERLEQKTETRFVSIDKVELIGAEVRDGAEQLTVRFISQMISVTRDKTGAIVDGNPEKISDITDVWTFARDISSRDPNWKLVGTGSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWI1</accession>
    <name>Q3SWI1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0092</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313429.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0092"/>
    <dbReference type="EnsemblBacteria" id="ABA03360">
        <property type="protein sequence ID" value="ABA03360"/>
        <property type="gene ID" value="Nwi_0092"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0092"/>
    <dbReference type="eggNOG" id="COG5468">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117986_1_0_5"/>
    <dbReference type="OMA" id="SACQVRP"/>
    <dbReference type="OrthoDB" id="1548553at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019867">
        <property type="term" value="C:outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043165">
        <property type="term" value="P:Gram-negative-bacterium-type cell outer membrane assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007485">
        <property type="entry name" value="LPS_assembly_LptE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04390">
        <property type="entry name" value="LptE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="194" mass="21148" checksum="E03EB27013831565" modified="2005-10-11" version="1">MWWGRSPARRSGERTGSRIALRIGVVAVLAALTAGCFRPMYAERGDGGPALRDRLMGVELLPIDKPNASPDARIGVALRNALAFKLYGNATGAPPTHQLKIRFGISRSSLMLDPRTALPSNESFGIDASYQLIEVATGKIVLNATTFARTSYDIPGQFQRFARARAYRDAEDRAAEQIAENINTRLASFFYAGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWI7</accession>
    <name>Q3SWI7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cyclic nucleotide-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0086</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0086"/>
    <dbReference type="EnsemblBacteria" id="ABA03354">
        <property type="protein sequence ID" value="ABA03354"/>
        <property type="gene ID" value="Nwi_0086"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0086"/>
    <dbReference type="eggNOG" id="COG0664">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075053_16_3_5"/>
    <dbReference type="OMA" id="TRALFHR"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="SSF51206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="26"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="160" mass="17574" checksum="0C693FE538D11A53" modified="2005-10-11" version="1">MIRDGSSNRFCMSIDDDVVLLERVPTLRLLGAEALRVLAIGAEQQQFASGSILFRAGDEADGGYVVQEGGFRIHVPNGRDRKVVAERGTLIGELALIVPISRPATAIAREYSMVIRIPRTLFLRVLDSYPAAAQRLRDEFANRTRQTTSDILMASAKLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMX3</accession>
    <name>Q3SMX3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_3121</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316276.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3121"/>
    <dbReference type="EnsemblBacteria" id="ABA06368">
        <property type="protein sequence ID" value="ABA06368"/>
        <property type="gene ID" value="Nwi_3121"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3121"/>
    <dbReference type="eggNOG" id="ENOG502Z9SP">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038463_0_0_5"/>
    <dbReference type="OMA" id="NLSEARW"/>
    <dbReference type="OrthoDB" id="1036927at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025359">
        <property type="entry name" value="DUF4263"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14082">
        <property type="entry name" value="DUF4263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="483" mass="53700" checksum="7A5CA113B928335F" modified="2005-10-11" version="1">MALGFRQHDPERDGTLEVEEYHPNFVGVYFVPPKGSLLAAGLDPTQAKSYRTKLLDIHGQYKFLTIHPISTFGGKSDFLKPKYGQIERITLDGTDIIFRGGDDGGVPTTPEGVLELLEELPSAFTKDYAYGLGLAKSYRFIIDAVESLSDSTEIAITDDHATGPDPDGNRIFYISRRDFEQARRCINSIDSLAQSAARSVKETATYNILAERLGVPTLDPKAGRHPYRKLFTAVAQGKEELSEEDQNAVIGALSNHAASIAEVQPEKLVKLRGDIELVTLEALTKRYEEMLGEKLVEGRWQVFFNENPFILNMAFGYPVIKVRDQASVGGRKLSGNGEKITDFLVKNSLTNNTAIFEIKTPQTAILNKTPFRDGVFTPSADLSGSINQALDQKYQFQKQIAQIKDNTRLYDIESYAVHCCLVIGKTPDGDDQNKSFELFRRNSKDVEIVTFDELLEKLKQLSVFLRVGDEPAKSQESKGNANG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN06</accession>
    <name>Q3SN06_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_3088</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3088"/>
    <dbReference type="EnsemblBacteria" id="ABA06335">
        <property type="protein sequence ID" value="ABA06335"/>
        <property type="gene ID" value="Nwi_3088"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3088"/>
    <dbReference type="HOGENOM" id="CLU_2094267_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046120">
        <property type="entry name" value="DUF6117"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19612">
        <property type="entry name" value="DUF6117"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="116" mass="12333" checksum="C4CC9D669E3B64C6" modified="2005-10-11" version="1">MAIPDYARTNFNTMLRAAGDGNLALMECLDAATREPRYVLCAVGRDGGDYVFTPLGHLACPPSGLLRQFRGFREGRISGSSQPIWSADETEIRAGESTGRAGREGHPPGNAPAVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN72</accession>
    <name>Q3SN72_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">YcjX-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_3019</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3019"/>
    <dbReference type="EnsemblBacteria" id="ABA06269">
        <property type="protein sequence ID" value="ABA06269"/>
        <property type="gene ID" value="Nwi_3019"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3019"/>
    <dbReference type="eggNOG" id="COG3106">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043657_0_0_5"/>
    <dbReference type="OMA" id="FDYPGEW"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007413">
        <property type="entry name" value="DUF463_YcjX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38605">
        <property type="entry name" value="PTHR38605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04317">
        <property type="entry name" value="DUF463"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019381">
        <property type="entry name" value="YcjX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="502" mass="55913" checksum="CA0DB1366685C755" modified="2005-10-11" version="1">MRHCRLHHRHHINMAASFSDIVEEARLSARALLDYGENFFNPTIRLGVTGLSRAGKTVFITALVHGLSRGGRFPVFESLASGRIARAQLAPQPDDAIPRFNYEHHVNTLIAERRWPSSTVDISELRLVIGYQRQNGSDQTLTLDIVDYPGEWLLDLPLLDKSYEQWSAESLALSRQQPRARLAQAWHAHLATMRPSAREDEQATLTAARLFTDYLRGCRDERFAMSLLPPGRFLMPGNLAGSPALTFAPLDLPAGGIPLDGSLWAMMRRRYEAYKDLVVRPFFRDHFSRLDRQIVLVDTLAAFNAGPEALYDLEAALAGILDCYRVGRSTLLSTLFRPRIDRILFAATKADHLHHVSHDRLEAILRRIVERAASRAEVAGATIDVVALAAVRATREAQVRCGREKLPSILGTPLAGEAANGETFDGNTEVATFPGDLPEDVTSLFSGNGAFRGLSTSAMDQADFRFLRFRPPRLQNEGTGEPSLPHIRLDRALQFLIGDRLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNJ3</accession>
    <name>Q3SNJ3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2898</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316078.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2898"/>
    <dbReference type="EnsemblBacteria" id="ABA06148">
        <property type="protein sequence ID" value="ABA06148"/>
        <property type="gene ID" value="Nwi_2898"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2898"/>
    <dbReference type="HOGENOM" id="CLU_1914866_0_0_5"/>
    <dbReference type="OrthoDB" id="2042006at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021647">
        <property type="entry name" value="CusF_Ec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042230">
        <property type="entry name" value="CusF_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11604">
        <property type="entry name" value="CusF_Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229111" evidence="1">
        <location>
            <begin position="25"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="132" mass="14593" checksum="3459BD8E4B9B0654" modified="2005-10-11" version="1">MRYRIFVWTIAATAVILDFGKAHAFSSGDGAAARARGTEFLLVQVHKAKNETRDRSDALFQAVGFVTAINPATGSLTINHEEIVGLMPPMEMVFQVEPPTLSEGVKPGDKVEFRIDGKTYRVRELKVIEQSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNM3</accession>
    <name>Q3SNM3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2868</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HipA Ser/Thr kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316050.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2868"/>
    <dbReference type="EnsemblBacteria" id="ABA06118">
        <property type="protein sequence ID" value="ABA06118"/>
        <property type="gene ID" value="Nwi_2868"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2868"/>
    <dbReference type="eggNOG" id="COG3550">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041102_0_0_5"/>
    <dbReference type="OMA" id="RICFNVL"/>
    <dbReference type="OrthoDB" id="1293681at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012893">
        <property type="entry name" value="HipA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017508">
        <property type="entry name" value="HipA_N1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13657">
        <property type="entry name" value="Couple_hipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07804">
        <property type="entry name" value="HipA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Couple_hipA" evidence="4">
        <location>
            <begin position="30"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="HipA_C" evidence="3">
        <location>
            <begin position="174"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA06118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="430" mass="48135" checksum="9AD2BD891C843E27" modified="2005-10-11" version="1">MISRVTPTECFVYITLPGQTAPVTAARFELTKDRRDVAIGRLVYGRSYLARAEAVPIDPIELRLAGQTYETVGMKGLFGALRDAGPDYWGRRVIERHVGAAQLNEMDYLLHSPDDRAGALGFGLNQQPPAPKRDFNKTLSLPRLQKIADAIVDEEDRPDDAEAAQVEELLLVVTSMGGARPKAVVEDTDGLWIAKFNRKDDKWNSARAEHAMLVLARACGVTTAESKVIVVGGRDVLMVKRFDREQTQGGYLRARMLSGLTLLRAEEGHDTRDRWSYVLLVEELRRISANPRADAAELFRRMCFNALISNTDDHPRNHAAVAMTPDWKLSPAYDLTPTPLISEERRDLAMICGDAGRYANAENIVSQSMRFLLEKDQAEKIVSDMEEQVRNKWYEVARREGLSEADCNTISRSFVYPGFRLPMSRAAPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNU8</accession>
    <name>Q3SNU8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2790</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315988.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2790"/>
    <dbReference type="EnsemblBacteria" id="ABA06043">
        <property type="protein sequence ID" value="ABA06043"/>
        <property type="gene ID" value="Nwi_2790"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2790"/>
    <dbReference type="eggNOG" id="COG3824">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123836_0_0_5"/>
    <dbReference type="OMA" id="GEWYAGV"/>
    <dbReference type="OrthoDB" id="1867074at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010428">
        <property type="entry name" value="Zincin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038555">
        <property type="entry name" value="Zincin_1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06262">
        <property type="entry name" value="Zincin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="133" mass="15364" checksum="E63F83C0FC5F4CBC" modified="2005-10-11" version="1">MWTSAKAPSLNEMETMAREAFARLPENFRRLCDGLIIRVEDFPTDEVLKEMQAESEFDLLGLFQGTGLPFQSHDDVARLPNMIWLYRRPIIDYWAEHDETLDHIISHVLIHEIGHHFGLSDADMEAIEAQAVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNX2</accession>
    <name>Q3SNX2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2766</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06019">
        <property type="protein sequence ID" value="ABA06019"/>
        <property type="gene ID" value="Nwi_2766"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2766"/>
    <dbReference type="eggNOG" id="ENOG5033MTW">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_212457_1_0_5"/>
    <dbReference type="OMA" id="LIGMLPI"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="51" mass="5627" checksum="1B2053D8A5132574" modified="2005-10-11" version="1">MFISVLITFFIVVLVLYFISVAPLDIRGKQIARVAAIVIGVASALRFVPMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNY5</accession>
    <name>Q3SNY5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DsbC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2753</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315951.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2753"/>
    <dbReference type="EnsemblBacteria" id="ABA06006">
        <property type="protein sequence ID" value="ABA06006"/>
        <property type="gene ID" value="Nwi_2753"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2753"/>
    <dbReference type="eggNOG" id="COG4233">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047910_1_1_5"/>
    <dbReference type="OMA" id="KTYWRDP"/>
    <dbReference type="OrthoDB" id="1211711at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028250">
        <property type="entry name" value="DsbDN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11412">
        <property type="entry name" value="DsbC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229002" description="DsbC domain-containing protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="DsbC" evidence="2">
        <location>
            <begin position="57"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="280" mass="29478" checksum="A8C1A418EB971446" modified="2005-10-11" version="1">MIIKVPLHALPGVAVVLLSCALSTAATGQDASPWQQGSHAAVRLLAGSRSGAVLLGGIAFQLEPGWKTFWRTPGDSGVPPRFDFSKSDNVDAVTVLWPAPSKFPDGAGGHSLGYKEQVVLPLRIAATNADRPITLRATVSFAVCARLCIPAEASMELAFASVASAEDSTLAAALDMVPKPARIGDPNPLTISNVRREGESTVTVDVATANPKDDVNLFVEGPTPDWALPIPTRHERAPRGVKRFSFKLDGLPANVNAQGAALKLTLVGGKQAYEFNINLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP37</accession>
    <name>Q3SP37_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2701</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2701"/>
    <dbReference type="EnsemblBacteria" id="ABA05954">
        <property type="protein sequence ID" value="ABA05954"/>
        <property type="gene ID" value="Nwi_2701"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2701"/>
    <dbReference type="eggNOG" id="COG5451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118542_0_0_5"/>
    <dbReference type="OMA" id="NEGAKWR"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012645">
        <property type="entry name" value="CHP02301"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09539">
        <property type="entry name" value="DUF2385"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02301">
        <property type="entry name" value="TIGR02301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="163" mass="18118" checksum="0F88A3E27C46BFC4" modified="2005-10-11" version="1">MRHGGLSGFEAGLKRLARIALPCFHRTEWAYHGGFGPFMRKSVLAIVLMTTICGALPARAQDGAAPFDAELQRLAEILGTLHYLRGICAANDGAKWRNEMQALIDAETPSGERRARMIAGFNRGYNWFQQTYRACTPAANVVIRRYVEEGAKISRDLTARYAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP71</accession>
    <name>Q3SP71_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2667</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2667"/>
    <dbReference type="EnsemblBacteria" id="ABA05920">
        <property type="protein sequence ID" value="ABA05920"/>
        <property type="gene ID" value="Nwi_2667"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2667"/>
    <dbReference type="eggNOG" id="ENOG5032YT5">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_178581_0_0_5"/>
    <dbReference type="OMA" id="FEVHAQG"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="92" mass="10357" checksum="11348BDCEC4D4125" modified="2005-10-11" version="1">MIMRDNVLKRPERKMSQCETCGNEYDKAFQVTMDGKTHTYDSFECAIHALAPVCGHCGIRIVGHGLESEGRFYCCDHCAENSGVKGLRDRNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP89</accession>
    <name>Q3SP89_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cytochrome c, class IC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2649</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315852.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2649"/>
    <dbReference type="EnsemblBacteria" id="ABA05902">
        <property type="protein sequence ID" value="ABA05902"/>
        <property type="gene ID" value="Nwi_2649"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2649"/>
    <dbReference type="eggNOG" id="COG2010">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101159_6_0_5"/>
    <dbReference type="OMA" id="DIYCAQC"/>
    <dbReference type="OrthoDB" id="1939800at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13442">
        <property type="entry name" value="Cytochrome_CBB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229150" evidence="5">
        <location>
            <begin position="24"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="6">
        <location>
            <begin position="22"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="118" mass="12880" checksum="F6751A88CA3538D0" modified="2005-10-11" version="1">MKTQMFAVFAGLALLLAPSTSRADQSIEQVYEVYCVQCHGLQRNGTGVNVPALSVKPRDHTDAKSMGDTPDDELFKAIKEGGLAVNKSVLMPTWGHVLSEDQIKGLVKYLRQVCKCGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPE3</accession>
    <name>Q3SPE3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Electron transfer flavoprotein-ubiquinone oxidoreductase</fullName>
            <shortName evidence="10">ETF-QO</shortName>
            <ecNumber evidence="10">1.5.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_2595</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 10">Accepts electrons from ETF and reduces ubiquinone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a ubiquinone + reduced [electron-transfer flavoprotein] = a ubiquinol + H(+) + oxidized [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:24052"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="1.5.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="10">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <dbReference type="EC" id="1.5.5.1" evidence="10"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315798.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2595"/>
    <dbReference type="EnsemblBacteria" id="ABA05848">
        <property type="protein sequence ID" value="ABA05848"/>
        <property type="gene ID" value="Nwi_2595"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2595"/>
    <dbReference type="eggNOG" id="COG0644">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2440">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009667_4_1_5"/>
    <dbReference type="OMA" id="GGGWMYH"/>
    <dbReference type="OrthoDB" id="1770293at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004174">
        <property type="term" value="F:electron-transferring-flavoprotein dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040156">
        <property type="entry name" value="ETF-QO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10617">
        <property type="entry name" value="PTHR10617"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 10" id="KW-0274">FAD</keyword>
    <keyword evidence="4 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="8 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <keyword evidence="9 10" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="11">
        <location>
            <begin position="513"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA05848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="553" mass="60091" checksum="B5617CEC5D409A07" modified="2005-10-11" version="1">MSSDELPPRESMDFDVVIVGAGPSGLSAAIRLKQLDSGLSIVVVEKGSEVGAHILSGAVIDPSGLDKLVPDWRDDADCPLKTKVRDDRFYWMTRGGAIRLPGFLMPPLMNNHRCYIGSLGNVCRWLARKAEALGVDIYPGFAAAEVLYDDDGAVRGIATGDMGIARDGSLKSSFTRGMELLGKYTLFAEGARGSLSKQLIARFQLDKTSDPPKFGIGLKEVWQVDPARHRNGLIQHSFGWPLNNSTGGGSFLYHYDDHRVAVGFVVHLNYDDPYLSPFDEFQRFKTHPAIRGVFEGGKRLAYGARAITEGGYQSVPRLSFPGGALVGCAAGFVNVPRIKGVHNAMGSGILAAEHVAAALGAGRANDEIAEYENAWRGSAIGTDLFKVRNVKPLWSKFGTVAGVALGGLDMWTNTLFGFSLLGTWPHTKPDRQTLDPARAHVRIAYPKPDGKLTFDKLSSVFLSNTNHEEDQPVHLKVTDTVLHKTSEHDVYAGPSSRYCPAGVYEWVEDGSDLRYQINAQNCVHCKTCDVKDPNGNITWVPPEGGGGPNYEAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPG3</accession>
    <name>Q3SPG3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2575</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315779.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2575"/>
    <dbReference type="EnsemblBacteria" id="ABA05828">
        <property type="protein sequence ID" value="ABA05828"/>
        <property type="gene ID" value="Nwi_2575"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2575"/>
    <dbReference type="eggNOG" id="ENOG5034B87">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_722928_0_0_5"/>
    <dbReference type="OMA" id="AVLDVCY"/>
    <dbReference type="OrthoDB" id="571862at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229208" evidence="2">
        <location>
            <begin position="22"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="345"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="399" mass="42716" checksum="B5F82D2CFB3FAEA4" modified="2005-10-11" version="1">MRALGLCLISLMIVLPATPRAQDRPLPRTTPGGAEVRYFTSIDGLMEGNADVILKETRHGKTVTSAVLDVCYPAEKGSDRKDRFVANLAVNGSTLTGSTQSIGDKLPVTVKLTRKPTGDTFEFDGRISVGERVTEVTSTDNSDLSEAEFNDVRTTDDGIIASPRDFTEVSPEAIAVKVKIDAATDLLKGLRGQNLRVLLNSLEVTCEALRAGAQVIDINIDPERAPALVARMKATPGVIAAGWSAGMFDMERTIRFPAAEWRDKGKLNRDKLAAAISGVLEKSLDAKPVSAKWSEDTGKLTLTFKRPSTLAPALGLTDTIEVTALPGPDRPEGSDHIMLWVGAPSTTTADESDGPRLGLADDLSTGDGESIEANDRGAMDALAKQFKAQRWDVDASAWK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPM7</accession>
    <name>PTH_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidyl-tRNA hydrolase</fullName>
            <shortName evidence="1">PTH</shortName>
            <ecNumber evidence="1">3.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pth</name>
        <name type="ordered locus">Nwi_2511</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an N-acyl-L-alpha-aminoacyl-tRNA + H2O = a tRNA + an N-acyl-L-amino acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:13883"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59874"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:138191"/>
            <dbReference type="EC" id="3.1.1.29"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PTH family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.29" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315715.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SPM7"/>
    <dbReference type="SMR" id="Q3SPM7"/>
    <dbReference type="STRING" id="323098.Nwi_2511"/>
    <dbReference type="EnsemblBacteria" id="ABA05764">
        <property type="protein sequence ID" value="ABA05764"/>
        <property type="gene ID" value="Nwi_2511"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2511"/>
    <dbReference type="eggNOG" id="COG0193">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062456_1_0_5"/>
    <dbReference type="OMA" id="HVLSKFH"/>
    <dbReference type="OrthoDB" id="1676462at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:aminoacyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00462">
        <property type="entry name" value="PTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00083">
        <property type="entry name" value="Pept_tRNA_hydro_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001328">
        <property type="entry name" value="Pept_tRNA_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018171">
        <property type="entry name" value="Pept_tRNA_hydro_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036416">
        <property type="entry name" value="Pept_tRNA_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17224">
        <property type="entry name" value="PTHR17224"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01195">
        <property type="entry name" value="Pept_tRNA_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53178">
        <property type="entry name" value="SSF53178"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00447">
        <property type="entry name" value="pth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01195">
        <property type="entry name" value="PEPT_TRNA_HYDROL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01196">
        <property type="entry name" value="PEPT_TRNA_HYDROL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000264067" description="Peptidyl-tRNA hydrolase">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00083"/>
        </source>
    </evidence>
    <sequence length="202" mass="22230" checksum="56918C6758265F93" modified="2005-10-11" version="1">MRLFVGLGNPGTKYQGNRHNIGFMVVDEIARRHGFAPWRRRFQGEASEGTLDRERVIVLKPATYMNNSGNAVQDAVQFFKLKEADVVVFHDEIELPPAKVRVKVGGGIAGHNGLRSISAHIGNDYRRVRLGVGHPGVKELVHAHVLNDFARSERSWVEALVDTVAENAALLVAAKDSAFQNKVHLAMQAKGFADNGGEDKQN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPN4</accession>
    <name>LGT_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphatidylglycerol--prolipoprotein diacylglyceryl transferase</fullName>
            <ecNumber evidence="1">2.5.1.145</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lgt</name>
        <name type="ordered locus">Nwi_2504</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of the diacylglyceryl group from phosphatidylglycerol to the sulfhydryl group of the N-terminal cysteine of a prolipoprotein, the first step in the formation of mature lipoproteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) + L-cysteinyl-[prolipoprotein] = H(+) + S-1,2-diacyl-sn-glyceryl-L-cysteinyl-[prolipoprotein] + sn-glycerol 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:56712"/>
            <dbReference type="Rhea" id="RHEA-COMP:14679"/>
            <dbReference type="Rhea" id="RHEA-COMP:14680"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:64716"/>
            <dbReference type="ChEBI" id="CHEBI:140658"/>
            <dbReference type="EC" id="2.5.1.145"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; lipoprotein biosynthesis (diacylglyceryl transfer).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Lgt family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.145" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315708.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SPN4"/>
    <dbReference type="SMR" id="Q3SPN4"/>
    <dbReference type="STRING" id="323098.Nwi_2504"/>
    <dbReference type="EnsemblBacteria" id="ABA05757">
        <property type="protein sequence ID" value="ABA05757"/>
        <property type="gene ID" value="Nwi_2504"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2504"/>
    <dbReference type="eggNOG" id="COG0682">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013386_1_0_5"/>
    <dbReference type="OMA" id="TDVPWAI"/>
    <dbReference type="OrthoDB" id="435911at2"/>
    <dbReference type="UniPathway" id="UPA00664"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008961">
        <property type="term" value="F:phosphatidylglycerol-prolipoprotein diacylglyceryl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042158">
        <property type="term" value="P:lipoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01147">
        <property type="entry name" value="Lgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001640">
        <property type="entry name" value="Lgt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30589">
        <property type="entry name" value="PTHR30589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01790">
        <property type="entry name" value="LGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00544">
        <property type="entry name" value="lgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01311">
        <property type="entry name" value="LGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_1000053461" description="Phosphatidylglycerol--prolipoprotein diacylglyceryl transferase">
        <location>
            <begin position="1"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol)</name>
            <dbReference type="ChEBI" id="CHEBI:64716"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01147"/>
        </source>
    </evidence>
    <sequence length="291" mass="31688" checksum="E219AF5212199549" modified="2005-10-11" version="1">MFLLITYPVFDPVAISLGPIAIRWYALAYIGGIMLGWLYARALLRSEKLWGGPAPISVVQLDDFILWVTIAIIVGGRVGYVLFYNPDYFIRYPAQIFQLWNGGMSFHGGFMGCVAAVILFCRRHGLPILSLGDVATAVGPIGLFLGRIANFINSELWGRPADPGLPWAMVFPNGGPLPRHPSQLYEAALEGILLFTILALMIRLGALKRPGLVLGSFIALYAMARIVAEFFREPDPQLGFLWGGLTMGMLLSIPMVIIGLAIVYAAWSRGSRASDAQASPNSGASTKVDRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPP7</accession>
    <name>Q3SPP7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GCN5-related N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2491</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315697.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2491"/>
    <dbReference type="EnsemblBacteria" id="ABA05744">
        <property type="protein sequence ID" value="ABA05744"/>
        <property type="gene ID" value="Nwi_2491"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2491"/>
    <dbReference type="eggNOG" id="COG1670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_1_0_5"/>
    <dbReference type="OMA" id="ELWKLWY"/>
    <dbReference type="OrthoDB" id="1374795at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="15"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="197" mass="22570" checksum="CC7C97E504139E32" modified="2005-10-11" version="1">MPWLQPVTLEGPHARLEPLSQDHREALVDAVKDGEIWKIWYTFVPAPEKMAAEIERRLSLQAAGTMLPFTVRDAEGRIAGMTTYMNVDSASCRVEIGSTWYAKRVQRTAVNTQCKLLLLTHAFEKLNCIAVEFRTHVFNHVSRRGLERLGARQDGTLRNRQMSPNVTLRDTVVYSILPGEWPKVKAHLSYELNEKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPR8</accession>
    <name>Q3SPR8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Helix-turn-helix, Fis-type</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2470</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315678.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2470"/>
    <dbReference type="EnsemblBacteria" id="ABA05723">
        <property type="protein sequence ID" value="ABA05723"/>
        <property type="gene ID" value="Nwi_2470"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2470"/>
    <dbReference type="eggNOG" id="COG2204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_188395_0_0_5"/>
    <dbReference type="OMA" id="CNGNRTH"/>
    <dbReference type="OrthoDB" id="123059at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002197">
        <property type="entry name" value="HTH_Fis"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02954">
        <property type="entry name" value="HTH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01590">
        <property type="entry name" value="HTHFIS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_8" evidence="1">
        <location>
            <begin position="33"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="84" mass="9304" checksum="19471C4420FDEB3F" modified="2005-10-11" version="1">MNQEIQITDQGSMKDQVALTPDEVIPLLVGSTVDEVERELVLQTLARCGGNRTRAARVLGVSVRTLRNKIRQYTSMGLDVPDHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ79</accession>
    <name>Q3SQ79_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2308</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315527.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05562">
        <property type="protein sequence ID" value="ABA05562"/>
        <property type="gene ID" value="Nwi_2308"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2308"/>
    <dbReference type="eggNOG" id="ENOG5031BSR">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2056358_0_0_5"/>
    <dbReference type="OMA" id="TMFTKAT"/>
    <dbReference type="OrthoDB" id="1820418at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229124" evidence="1">
        <location>
            <begin position="25"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="117" mass="12470" checksum="D491693A5F5A863B" modified="2005-10-11" version="1">MRTFTFTLSTAIALLVAGSPMALAQTSPTQSQTETQKGVAIRSIQVVDMDELKADVRSKVDAIVANTKQEDMKSLRKTIDATPEAVSALTAKGRNSAQVVAINIDQNGVLTMFTKAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ91</accession>
    <name>Q3SQ91_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">TonB-dependent receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_2296</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell outer membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315517.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2296"/>
    <dbReference type="EnsemblBacteria" id="ABA05550">
        <property type="protein sequence ID" value="ABA05550"/>
        <property type="gene ID" value="Nwi_2296"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2296"/>
    <dbReference type="eggNOG" id="COG4206">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008287_19_4_5"/>
    <dbReference type="OMA" id="NNKVFHH"/>
    <dbReference type="OrthoDB" id="439303at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039426">
        <property type="entry name" value="BtuB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012910">
        <property type="entry name" value="Plug_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037066">
        <property type="entry name" value="Plug_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011662">
        <property type="entry name" value="Secretin/TonB_short_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036942">
        <property type="entry name" value="TonB_rcpt_b-brl_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30069">
        <property type="entry name" value="PTHR30069"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07715">
        <property type="entry name" value="Plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00965">
        <property type="entry name" value="STN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229196" evidence="7">
        <location>
            <begin position="32"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="domain" description="STN" evidence="8">
        <location>
            <begin position="59"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="752" mass="81358" checksum="7598720E50E42BC8" modified="2005-10-11" version="1">MRKFTSAAVLRCGVSICSVTLLVAGAHEAWARDDIAYSFSISAKPRLSALADFTAATGIQVVRPDAGAIRGISRAVSGRYSAETALREILAGSNLGYHFTGPRTVAIEAPGAPAGYGADVVTLDTISVTGGAQDIGRGGVSEINITRADIERRNATDVKGLFRGEAGITVGSPLPMSQKVYVHGIEETNLAVSIDGARQNNKVFHHNATTMIDPNLLKAVRIDAGVAPVDSGAGALAGAIAYVTKDARDFLSEDGFGGSLKSIFNFNGHVSTNNLTGYSRQGGFETLGSLTYGRGNEFRAGNGQEVLGTATNFLSGIGKVAYQGIEGQRFAISHEQMRDDAIRPFRANAAQIVDNRSTPLLRPYTLDRQNTVFTYTDASPEGWWDPKFVLAYTRSKVAVDQYSGATIDTYSYTSEGITDSFNGKLENKFALPIGNIVAGLDFYRDRAEYLDKSYRSAERASNFGAYAQARLRPWVRTRLSFGLRGEHQNFEGVNGFRSSGQGLSGNVSGEYDLTSFLIAKAGFSHVWAGIPLAENYAQNPAWNYGAGPKSVTSNNYTTGLVAHYGSLQVEGGVFRTQINDARVPLWAPDMALRAFNLQTQGFQAGGTYNWADGFVRVRFIRIDAEIDGRPADSYVGRYLTAPIGDVLTFQAVHTIAPWKLTVGGDVEIVFDNNTLFNPATGIGRLEGYQVLNAFIEHRSFYLPTLTMRGEIRNLFNATYTARGTYGLEFGTGLVRPLYESGRSFLASLRLDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQD1</accession>
    <name>Q3SQD1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA-directed DNA polymerase</fullName>
            <ecNumber evidence="2">2.7.7.7</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2256</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.7" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2256"/>
    <dbReference type="EnsemblBacteria" id="ABA05510">
        <property type="protein sequence ID" value="ABA05510"/>
        <property type="gene ID" value="Nwi_2256"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2256"/>
    <dbReference type="eggNOG" id="COG0389">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028184_1_1_5"/>
    <dbReference type="OMA" id="HRPECAW"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001126">
        <property type="entry name" value="UmuC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00817">
        <property type="entry name" value="IMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="UmuC" evidence="1">
        <location>
            <begin position="2"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="501" mass="55209" checksum="7A1C7ED59C317A01" modified="2005-10-11" version="1">MVISALNDAAARLGLTIGLPLANARAICPDIDVFDADETADARTLNGIADWCDRFTPLVALDPPHGLLLDITGCAHLFGGEAALMNALCSALTRQGFAVSAAIAGTPACARALTRHVHGRIVAPGEELEAVAPLPVFTLGTDDEITRGLRRAGLKTIGDVAVRGRHEIAARFGAAFTALLEQTLGQRDAPINPRRPPPDYIVEKRFAEPIATDSAIAMTLAGLARTLIAAMEKQGKGARHLEAGFFRTDGAVRVITVETGRPVTRGEIVDRLFRERLDALADPLDPGFGFDLVRLSAVRTESVVHEQRDLDARVQDNDEVAALIDRIAARIGSHRVVVHLPQDTHIPERGALSLPAQQHFVMAMRAEWPARIGSEPPLRPLRMFARPEPIEVTAMFPDGPPAQFKWRHRQCLIAHVEGPERIAMEWWNRCERALSPEPDDEFMRLRPPRGEVAVPRADGHALTRDYFRAEDKGGVRYWLYREGIYGREVQTFRWFLHGMFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQF2</accession>
    <name>Q3SQF2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase</fullName>
            <ecNumber evidence="3">2.7.6.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Nwi_2235</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the transfer of pyrophosphate from adenosine triphosphate (ATP) to 6-hydroxymethyl-7,8-dihydropterin, an enzymatic step in folate biosynthesis pathway.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HPPK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.3" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315457.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2235"/>
    <dbReference type="EnsemblBacteria" id="ABA05489">
        <property type="protein sequence ID" value="ABA05489"/>
        <property type="gene ID" value="Nwi_2235"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2235"/>
    <dbReference type="eggNOG" id="COG0801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097916_2_3_5"/>
    <dbReference type="OMA" id="QVILRQN"/>
    <dbReference type="OrthoDB" id="1953248at2"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00155"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003848">
        <property type="term" value="F:2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00483">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000550">
        <property type="entry name" value="Hppk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035907">
        <property type="entry name" value="Hppk_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01288">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55083">
        <property type="entry name" value="SSF55083"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01498">
        <property type="entry name" value="folK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00794">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="7 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HPPK" evidence="13">
        <location>
            <begin position="90"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS00794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABA05489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="163" mass="17893" checksum="3773740FE2170121" modified="2005-10-11" version="1">MASVLIALGGNVGDVRQTFRKAIANIRGMAQAALLARSSDYATPPWGEEKQARFVNACIEIDTGLDPHALLFTLQKIEKKFGRDRAAEQRWGPRTLDLDLIAYDDAILDKPELTLPHPRLFERAFVLVPLAEIAPDRIIAGQTPARALADISTAGIERLPDLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQG5</accession>
    <name>Q3SQG5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2222</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315445.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2222"/>
    <dbReference type="EnsemblBacteria" id="ABA05476">
        <property type="protein sequence ID" value="ABA05476"/>
        <property type="gene ID" value="Nwi_2222"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2222"/>
    <dbReference type="eggNOG" id="ENOG5032Z7R">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128167_0_0_5"/>
    <dbReference type="OMA" id="MCSEKPE"/>
    <dbReference type="OrthoDB" id="1841771at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035220">
        <property type="entry name" value="DUF5330"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17264">
        <property type="entry name" value="DUF5330"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="138" mass="14720" checksum="ECEF5C0E6DD4B38B" modified="2005-10-11" version="1">MFFLLRMAFWLGLVLVLLPRDEAPEANKAPRIGASEAVSAATAAVSDMTQFCTRQPAACEVGGQAAAVIGQRAQDGAKKLYRIITDKRPPDHTGSIDTVPAVETAASGDAQHDTLTPEDLQAEWRLAAPNAVPREQIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQH0</accession>
    <name>Q3SQH0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2217</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315440.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05471">
        <property type="protein sequence ID" value="ABA05471"/>
        <property type="gene ID" value="Nwi_2217"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2217"/>
    <dbReference type="eggNOG" id="ENOG5032KUU">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_709104_0_0_5"/>
    <dbReference type="OMA" id="QTNAIFQ"/>
    <dbReference type="OrthoDB" id="788083at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018759">
        <property type="entry name" value="BBP2_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10082">
        <property type="entry name" value="BBP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229201" evidence="1">
        <location>
            <begin position="23"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="400" mass="42893" checksum="2ECB4DCA4BC6A12D" modified="2005-10-11" version="1">MHIPKVFLLAGCLGLAGSHACAENDFTASFRLRGGFDTNPLLAIGQGGGSLFVAADTALAAGMKTDGYTLGVAAEATAMHYAQQRITPTRGGKVILRGSIGDDDLRLDSTSTIADVSSYTLRSSELIQSVRAESRRGPIKLFATAEGGRSSLNQANAIFQDFLPDPQQHLRGTLIPGVSLVSGKFEIGASVNLSARRYLQEFDVFGYRRDNERVQPFLFAKYGGDAVTAFGSVSRLYGTWHDPDFTNVDRILFDASLSWRAAPFGIDLTAWRRASETTFPISPITIDTAYSARVSWNWEPKMTLAASVGYSISEYLDSPFRARTLTCGVGAIRDFDHDLSVSIDVAYARGLLISGDQSNGLVVMSSITKRFSAAPPEPNKAASAAPASCGRGCPVRLPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQK2</accession>
    <name>Q3SQK2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2185</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315408.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05439">
        <property type="protein sequence ID" value="ABA05439"/>
        <property type="gene ID" value="Nwi_2185"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2185"/>
    <dbReference type="HOGENOM" id="CLU_196011_0_0_5"/>
    <dbReference type="OMA" id="TDYRVYI"/>
    <dbReference type="OrthoDB" id="2100413at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="60" mass="6776" checksum="D2D96A83DBC62063" modified="2005-10-11" version="1">MTDYRVYILNDKGHIQSDTLIVSDDDAQAIEAAKKLVGVRHVEMWDGERLVGRFKTTPDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQM0</accession>
    <name>Q3SQM0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">Adenylosuccinate lyase</fullName>
            <shortName evidence="10">ASL</shortName>
            <ecNumber evidence="4 10">4.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 10">Adenylosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_2167</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + fumarate</text>
            <dbReference type="Rhea" id="RHEA:23920"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:23921"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N(6)-(1,2-dicarboxyethyl)-AMP = AMP + fumarate</text>
            <dbReference type="Rhea" id="RHEA:16853"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:16854"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.2" evidence="4 10"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315390.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2167"/>
    <dbReference type="EnsemblBacteria" id="ABA05421">
        <property type="protein sequence ID" value="ABA05421"/>
        <property type="gene ID" value="Nwi_2167"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2167"/>
    <dbReference type="eggNOG" id="COG0015">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030949_0_1_5"/>
    <dbReference type="OMA" id="ASSCEKI"/>
    <dbReference type="OrthoDB" id="347727at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00132"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00336"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070626">
        <property type="term" value="F:(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004018">
        <property type="term" value="F:N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019468">
        <property type="entry name" value="AdenyloSucc_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004769">
        <property type="entry name" value="Pur_lyase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10397">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00998">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00928">
        <property type="entry name" value="purB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 10" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADSL_C" evidence="11">
        <location>
            <begin position="352"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA05421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="435" mass="48593" checksum="06B7BF4499A86E1C" modified="2005-10-11" version="1">MIPRYARPEMVSIWEPQTRFKIWFEIEAHAADAQAELGVIPKEAAEIIWAKAKDATFDIQRIDEIERETRHDVIAFLTHLAEIVGPEARFVHQGMTSSDVLDTCLNVQLSRAADILIADIDKLLGALKTRAFEHKMTPTIGRSHGIHAEPVTFGLKLAYAYAEFSRARERLTAARREVATCAISGAVGTFAQIDPRVEEHVAGAMGLIPEPVSTQVIPRDRHAMFFATLGVIASSMERLAIEIRHLQRTEVLEAEEFFSEGQKGSSAMPHKRNPVLTENITGLARMVRAYVTPALENVALWHERDISHSSVERMIGPDATVTLDFALNRLAGVIDKLLVYPANMQKNLDRLGGLVHSQRVLIALTQKGASREDAYKLVQRNAMPVWRGEGDFETLLKNDADVTKYLTGAEIAEQFDLNYHLKHVDTIFKRVFGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="126" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQS2</accession>
    <name>Q3SQS2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Co-chaperonin GroES</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">10 kDa chaperonin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Chaperonin-10</fullName>
            <shortName evidence="3">Cpn10</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">groES</name>
        <name evidence="3" type="synonym">groS</name>
        <name evidence="5" type="ordered locus">Nwi_2114</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Together with the chaperonin GroEL, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding. GroES binds to the apical surface of the GroEL ring, thereby capping the opening of the GroEL channel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heptamer of 7 subunits arranged in a ring. Interacts with the chaperonin GroEL.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3 4">Belongs to the GroES chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315342.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2114"/>
    <dbReference type="EnsemblBacteria" id="ABA05369">
        <property type="protein sequence ID" value="ABA05369"/>
        <property type="gene ID" value="Nwi_2114"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2114"/>
    <dbReference type="eggNOG" id="COG0234">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132825_1_0_5"/>
    <dbReference type="OMA" id="RSINMTI"/>
    <dbReference type="OrthoDB" id="1965002at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00320">
        <property type="entry name" value="cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.33.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00580">
        <property type="entry name" value="CH10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020818">
        <property type="entry name" value="Chaperonin_GroES"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037124">
        <property type="entry name" value="Chaperonin_GroES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018369">
        <property type="entry name" value="Chaprnonin_Cpn10_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772">
        <property type="entry name" value="PTHR10772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00166">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00297">
        <property type="entry name" value="CHAPERONIN10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00883">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00681">
        <property type="entry name" value="CHAPERONINS_CPN10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="104" mass="11294" checksum="7BFC6EAD45820385" modified="2005-10-11" version="1">MHFRPLHDRVVVRRIDAEEKTTGGIIIPDTAKEKPQEGEIVAAGPGARNEKGELVPLDVKAGDHILFGKWSGTEVKIDGEELLIMKESDILGVVEKVGTIKKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQT5</accession>
    <name>Q3SQT5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Helix-turn-helix, Fis-type</fullName>
            <ecNumber evidence="3">2.7.7.49</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2082</name>
        <name evidence="3" type="ordered locus">Nwi_2099</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="3">Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2082"/>
    <dbReference type="EnsemblBacteria" id="ABA05339">
        <property type="protein sequence ID" value="ABA05339"/>
        <property type="gene ID" value="Nwi_2082"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05356">
        <property type="protein sequence ID" value="ABA05356"/>
        <property type="gene ID" value="Nwi_2099"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2082"/>
    <dbReference type="KEGG" id="nwi:Nwi_2099"/>
    <dbReference type="eggNOG" id="COG2801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2963">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027402_10_0_5"/>
    <dbReference type="OMA" id="PYDVHHG"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010921">
        <property type="entry name" value="Trp_repressor/repl_initiator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48295">
        <property type="entry name" value="SSF48295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="132"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="326" mass="37802" checksum="E4F271ECF4A7E2FA" modified="2005-10-11" version="1">MRYPASEKAEIIRLVEASHLPARRTLDKLGIPRATFYRWYDRYLTGGIEALADHRSRPDRVWNRIPDPIRAELVELALRETELSPRELAVRFTDEKRYFVSEASVYRLLKAHDLITSPAYIVIKAASEFKDKTTAPNQLWQTDFTYLKITGWGWYYLSTVLDDFSRFIVAWKLCATMRADDVTATLDLALAASGLDQITVAHRPRLLSDNGASYISAELATWLDGKGMKHVRGAPYHPQTQGKIERWHQTLKNRILLENYYLPGDLERQVAAFVEHYNHGRYHESIDNLTPADVYFGRGQTILTERERIKRQTIHQRRLQHHLQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQU4</accession>
    <name>Q3SQU4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315325.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2090"/>
    <dbReference type="EnsemblBacteria" id="ABA05347">
        <property type="protein sequence ID" value="ABA05347"/>
        <property type="gene ID" value="Nwi_2090"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2090"/>
    <dbReference type="eggNOG" id="COG5639">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_179036_1_1_5"/>
    <dbReference type="OMA" id="PMVERFM"/>
    <dbReference type="OrthoDB" id="1902903at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018733">
        <property type="entry name" value="DUF2274"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10038">
        <property type="entry name" value="DUF2274"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="78" mass="8513" checksum="CBFEF99333207D78" modified="2005-10-11" version="1">MTKLRLSALPDDKAVKMTVELPAAVHRDLVAYAEILARTSGDAVPPDPAKLVAPMLQRFMATDKAFRKARAQLPSSRP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-01-10" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR23</accession>
    <name>PLSY_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycerol-3-phosphate acyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Acyl-PO4 G3P acyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Acyl-phosphate--glycerol-3-phosphate acyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">G3P acyltransferase</fullName>
            <shortName evidence="1">GPAT</shortName>
            <ecNumber evidence="1">2.3.1.275</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Lysophosphatidic acid synthase</fullName>
            <shortName evidence="1">LPA synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">plsY</name>
        <name type="ordered locus">Nwi_2009</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of an acyl group from acyl-phosphate (acyl-PO(4)) to glycerol-3-phosphate (G3P) to form lysophosphatidic acid (LPA). This enzyme utilizes acyl-phosphate as fatty acyl donor, but not acyl-CoA or acyl-ACP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an acyl phosphate + sn-glycerol 3-phosphate = a 1-acyl-sn-glycero-3-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:34075"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57970"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="EC" id="2.3.1.275"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; phospholipid metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Probably interacts with PlsX.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PlsY family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.275" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315254.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SR23"/>
    <dbReference type="SMR" id="Q3SR23"/>
    <dbReference type="STRING" id="323098.Nwi_2009"/>
    <dbReference type="EnsemblBacteria" id="ABA05268">
        <property type="protein sequence ID" value="ABA05268"/>
        <property type="gene ID" value="Nwi_2009"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2009"/>
    <dbReference type="eggNOG" id="COG0344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081254_1_0_5"/>
    <dbReference type="OMA" id="WRHRGNL"/>
    <dbReference type="OrthoDB" id="1691856at2"/>
    <dbReference type="UniPathway" id="UPA00085"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043772">
        <property type="term" value="F:acyl-phosphate glycerol-3-phosphate acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01043">
        <property type="entry name" value="PlsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003811">
        <property type="entry name" value="G3P_acylTferase_PlsY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30309">
        <property type="entry name" value="PTHR30309"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02660">
        <property type="entry name" value="G3P_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01207">
        <property type="entry name" value="G3P_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00023">
        <property type="entry name" value="TIGR00023"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000188412" description="Glycerol-3-phosphate acyltransferase">
        <location>
            <begin position="1"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01043"/>
        </source>
    </evidence>
    <sequence length="196" mass="20708" checksum="8A6E363B4E4C63AE" modified="2005-10-11" version="1">MFPNGLIAFAFGYLLGSIPFGMILTRIAGTQDLRSIGSGNIGATNVLRTGRRGLAAATLLGDMLKGTAAVVIALLLSGPGAAMAATLGAFLGHLFPVWLKFRGGKGVATYIGVLLGLFWPAALAFCAIWLLVAFTTRYSSLSALLASLTTPLLLWGFGHPQFALLFAVLTVLLWFKHRENIRRLFAGSEGRIGAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR46</accession>
    <name>Q3SR46_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribulose bisphosphate carboxylase small subunit</fullName>
            <shortName evidence="3">RuBisCO small subunit</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cbbS</name>
        <name evidence="5" type="ordered locus">Nwi_1986</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site. Although the small subunit is not catalytic it is essential for maximal activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterohexadecamer of 8 large and 8 small subunits.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="3">The basic functional RuBisCO is composed of a large chain homodimer in a 'head-to-tail' conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric 'cap' on each end of the 'barrel'.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RuBisCO small chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315232.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="SMR" id="Q3SR46"/>
    <dbReference type="STRING" id="323098.Nwi_1986"/>
    <dbReference type="EnsemblBacteria" id="ABA05245">
        <property type="protein sequence ID" value="ABA05245"/>
        <property type="gene ID" value="Nwi_1986"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1986"/>
    <dbReference type="eggNOG" id="COG4451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098114_2_0_5"/>
    <dbReference type="OMA" id="WNPAIEH"/>
    <dbReference type="OrthoDB" id="1708389at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016984">
        <property type="term" value="F:ribulose-bisphosphate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019253">
        <property type="term" value="P:reductive pentose-phosphate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03527">
        <property type="entry name" value="RuBisCO_small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00859">
        <property type="entry name" value="RuBisCO_S_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024681">
        <property type="entry name" value="RuBisCO_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000894">
        <property type="entry name" value="RuBisCO_ssu_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036385">
        <property type="entry name" value="RuBisCO_ssu_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31262">
        <property type="entry name" value="PTHR31262"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00101">
        <property type="entry name" value="RuBisCO_small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00961">
        <property type="entry name" value="RuBisCO_small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55239">
        <property type="entry name" value="SSF55239"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0113">Calvin cycle</keyword>
    <keyword evidence="2 3" id="KW-0120">Carbon dioxide fixation</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RuBisCO_small" evidence="4">
        <location>
            <begin position="11"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="108" mass="12555" checksum="C34B1D23B24FABB1" modified="2005-10-11" version="1">MAVQAYRSLKKYETFSYLPQMTPEQVRRQIAHAIAQGWNPAVEHTEKGTPAKASYWYMWKLPLFGEQSVDAVVAEIEACHREFPDQMVRFVAYDNYAQSQGMAFVVYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR58</accession>
    <name>Q3SR58_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1974</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315220.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1974"/>
    <dbReference type="EnsemblBacteria" id="ABA05233">
        <property type="protein sequence ID" value="ABA05233"/>
        <property type="gene ID" value="Nwi_1974"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1974"/>
    <dbReference type="eggNOG" id="COG4577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091281_0_0_5"/>
    <dbReference type="OMA" id="WIEVAPG"/>
    <dbReference type="OrthoDB" id="1374667at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031469">
        <property type="term" value="C:bacterial microcompartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1710">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044870">
        <property type="entry name" value="BMC_CP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000249">
        <property type="entry name" value="BMC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037233">
        <property type="entry name" value="CcmK-like_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00877">
        <property type="entry name" value="BMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143414">
        <property type="entry name" value="SSF143414"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51931">
        <property type="entry name" value="BMC_CP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1283">Bacterial microcompartment</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BMC circularly permuted" evidence="2">
        <location>
            <begin position="4"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="BMC circularly permuted" evidence="2">
        <location>
            <begin position="107"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="210" mass="23235" checksum="2B0B518ECB6E5DEB" modified="2005-10-11" version="1">MKITLRTYVFMDALQPQLASYIATSSQGFLPVPGDACMWIEVAPGMAVHNLSDIALKKTNVRMGEQVVERAFGSMELHFRNQSEVLAAGELILRQLNTTMDSRLRCKIAWNEIIRAITPDHATLINRQLRKGSMILPGKSMFILEVEPAGYIMYAANEAEKAAHITLVDVKAFGSFGRLTMMGNEAEAEEAQRAAVAAIERLNQRAMAHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR90</accession>
    <name>Q3SR90_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">CRISPR-associated helicase, Cas3 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_1941</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the CRISPR-associated nuclease Cas3-HD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the central section; belongs to the CRISPR-associated helicase Cas3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315194.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1941"/>
    <dbReference type="EnsemblBacteria" id="ABA05201">
        <property type="protein sequence ID" value="ABA05201"/>
        <property type="gene ID" value="Nwi_1941"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1941"/>
    <dbReference type="eggNOG" id="COG1203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010123_0_0_5"/>
    <dbReference type="OMA" id="HLSTNMC"/>
    <dbReference type="OrthoDB" id="1149839at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035011">
        <property type="entry name" value="Cas3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006483">
        <property type="entry name" value="CRISPR-assoc_Cas3_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038257">
        <property type="entry name" value="CRISPR-assoc_Cas3_HD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006474">
        <property type="entry name" value="Helicase_Cas3_CRISPR-ass_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF5">
        <property type="entry name" value="PTHR47959:SF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01587">
        <property type="entry name" value="cas3_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01596">
        <property type="entry name" value="cas3_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51643">
        <property type="entry name" value="HD_CAS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD Cas3-type" evidence="10">
        <location>
            <begin position="12"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="238"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA05201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="744" mass="82906" checksum="D1F22EA0AE0508CB" modified="2005-10-11" version="1">MTYHAHSGKLTDESDWQILSHHLTRVAARAGIYGSPIGLEGLAKIAGLFHDLGKYTADFQRRLHGINLRVDHSTAGAAVLMKMVPRPVREIAELVAYTILGHHAGLPDKFNEFGHCFLRRVREFEDRLDPVWKDQLSFDLGDLQMRELMGKLSPEKRIAEFELSVVTRMLFSCLVDADFKDTEAFYDALEGRQSNREWPLLQDVLPAFLAAFDAHMAAKSKDGEVNRLRGDILAHVRAGALNEPGLFTLNVPTGGGKTLASLGFALAHARKWDHRRIIYAIPFTSIVDQTAAIFRDILGEDNVLEHHSAIDEEHIEERWGRDKLKLAMQDWAAPVVVTTNVQFFESLFAARTSRARKLHNIAGSIIILDEAQTIPRPLLKPCVRMLDALARLFGCTIVLCTATQPALDASNFPDGLKLDGRELAPDPGKLSARLKRARIVRVGAMNNPELIEAIRAEPQALFIVNSRKHALDLYKEGKNAGVDGLVHLTTRQCAAHRRLILGDVKARLKNGETCRLVATSLIEAGVDVDFPGVWRAEAGLDQIVQAAGRCNREGRHPVEDSIVSVFSAPDYPPPREIAGLIGDMGRVIPKHEDLLSLGAIADYFGEVYWRAGPELDAKKILEGFKINRDGTDFAFRCVAEKFRMIESGMEPVIVEFDNDAEETVTELEFERISSGVLARKLQSYIVQVPPKARRLLIDNGHVAFVRPDIRGDQFAVLKNASLYRQEVGLMWEDAAYLAAESWQI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRA3</accession>
    <name>MSCL_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large-conductance mechanosensitive channel</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mscL</name>
        <name type="ordered locus">Nwi_1928</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homopentamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MscL family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315184.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRA3"/>
    <dbReference type="STRING" id="323098.Nwi_1928"/>
    <dbReference type="EnsemblBacteria" id="ABA05188">
        <property type="protein sequence ID" value="ABA05188"/>
        <property type="gene ID" value="Nwi_1928"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1928"/>
    <dbReference type="eggNOG" id="COG1970">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095787_0_1_5"/>
    <dbReference type="OMA" id="AWIIFLM"/>
    <dbReference type="OrthoDB" id="1863650at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00115">
        <property type="entry name" value="MscL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019823">
        <property type="entry name" value="Mechanosensitive_channel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001185">
        <property type="entry name" value="MS_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037673">
        <property type="entry name" value="MSC/AndL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036019">
        <property type="entry name" value="MscL_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30266">
        <property type="entry name" value="PTHR30266"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01741">
        <property type="entry name" value="MscL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01264">
        <property type="entry name" value="MECHCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81330">
        <property type="entry name" value="SSF81330"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00220">
        <property type="entry name" value="mscL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01327">
        <property type="entry name" value="MSCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0407">Ion channel</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000238013" description="Large-conductance mechanosensitive channel">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="19"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00115"/>
        </source>
    </evidence>
    <sequence length="139" mass="15106" checksum="77D9F2997314D71F" modified="2005-10-11" version="1">MWKEFREFAMKGNVVDLAVGVIIGAAFGGIVSSMVADIIMPIVGAVTGGLDFSNYFLPLSESVNASNLSDAKKQGAVLAWGNFLTLTLNFLIVAFVLFMVIKGMNRLKRKDEAASAEPPKPTREEELLTEIRDLLKAKV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-21" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRB3</accession>
    <name>RNC_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease 3</fullName>
            <ecNumber evidence="1">3.1.26.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Ribonuclease III</fullName>
            <shortName evidence="1">RNase III</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnc</name>
        <name type="ordered locus">Nwi_1918</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribonuclease III family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.3" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315174.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRB3"/>
    <dbReference type="SMR" id="Q3SRB3"/>
    <dbReference type="STRING" id="323098.Nwi_1918"/>
    <dbReference type="EnsemblBacteria" id="ABA05178">
        <property type="protein sequence ID" value="ABA05178"/>
        <property type="gene ID" value="Nwi_1918"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1918"/>
    <dbReference type="eggNOG" id="COG0571">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000907_1_1_5"/>
    <dbReference type="OMA" id="LTHKSCK"/>
    <dbReference type="OrthoDB" id="1890943at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00104">
        <property type="entry name" value="RNase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011907">
        <property type="entry name" value="RNase_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207">
        <property type="entry name" value="PTHR11207"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14622">
        <property type="entry name" value="Ribonucleas_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="SSF69065"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02191">
        <property type="entry name" value="RNaseIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00517">
        <property type="entry name" value="RNASE_3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0507">mRNA processing</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000228554" description="Ribonuclease 3">
        <location>
            <begin position="1"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="RNase III" evidence="1">
        <location>
            <begin position="43"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="1">
        <location>
            <begin position="196"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="266" mass="28671" checksum="4988B9A36D827F38" modified="2005-10-11" version="1">MMDESADIKPVPTSEDVAAPSGTEPVAPAPKKKRARTSTKAIMAAIEQRLGHTFADISLLTTAFTHVSALKSSRGRCDSYQRLEFLGDHVLGLVVSDMLYRAFPKADEGELSKRLADLVRKETCIDVAKSLDLQEGVKLGAVGAGAGARLRRSVLGDICEAVIGAVYLDGGYAAAAAFVERNWLERMRKPVRPLRDPKTVLQEWAQGKGLPTPVYREVERTGPHHDPRFRVAVELPGLESSEGIGGSKRAAEKAAASAMIVREGVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRI4</accession>
    <name>Q3SRI4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1847</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1847"/>
    <dbReference type="EnsemblBacteria" id="ABA05107">
        <property type="protein sequence ID" value="ABA05107"/>
        <property type="gene ID" value="Nwi_1847"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1847"/>
    <dbReference type="eggNOG" id="COG3494">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085042_1_0_5"/>
    <dbReference type="OMA" id="CKPQQDI"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010415">
        <property type="entry name" value="LpxI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043167">
        <property type="entry name" value="LpxI_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041255">
        <property type="entry name" value="LpxI_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06230">
        <property type="entry name" value="LpxI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17930">
        <property type="entry name" value="LpxI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LpxI_N" evidence="2">
        <location>
            <begin position="15"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="LpxI_C" evidence="1">
        <location>
            <begin position="147"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="288" mass="30100" checksum="0CFDEA927FCF904D" modified="2005-10-11" version="1">MPVMTDRVSGMSSPIGLIAGGGVLPFAVADSLTARGLKPVFFALKGVCDPERVSRFPHHWIAVGQIGKVMRLLRAENCRDLVFIGTLVRPALSEIRLDWGTIRAMGQVLAAFRGGDDHLLSGVGRILERDGFRMVGIKDIAPDLLMPAGCLTRKAPDQSAAADIAKGLDVLRALSPFDVGQAVVVIDAHVVGVEGIEGTDALLARIAQLRAAGRIRAKAPRGVLVKAPKHGQDLRYDLPTLGPRTIANAAAAGLAGLAVVAGNTLVAEPQALVGEADASNLFVVGLPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRL8</accession>
    <name>Q3SRL8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1812</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1812"/>
    <dbReference type="EnsemblBacteria" id="ABA05073">
        <property type="protein sequence ID" value="ABA05073"/>
        <property type="gene ID" value="Nwi_1812"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1812"/>
    <dbReference type="HOGENOM" id="CLU_2118482_0_0_5"/>
    <dbReference type="OrthoDB" id="156648at356"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="114" mass="12652" checksum="B285C238E30818E8" modified="2005-10-11" version="1">MDVGTDVKGRLSSRRVAEGPMRAPGLAGLSGARWIDSHRLAALATADGVRNDSKAGRPRTDVNLIYAGYPDRKTEWRIGATNPTSDERAKFARQFEQMFSRILTCTGRVFEKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRQ6</accession>
    <name>Q3SRQ6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Response regulator receiver</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1774</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315031.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1774"/>
    <dbReference type="EnsemblBacteria" id="ABA05035">
        <property type="protein sequence ID" value="ABA05035"/>
        <property type="gene ID" value="Nwi_1774"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1774"/>
    <dbReference type="eggNOG" id="COG0784">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_11_5"/>
    <dbReference type="OMA" id="RCVAKPF"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="10"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="126" mass="13522" checksum="2C1B225956127751" modified="2005-10-11" version="1">MESAKPSGGSVFLVEDEVMIRMMVADMLEELGYSIAAEAGDIEQALQLARTTNFDIAILDVNVNGKLISPVAEVIEGRNRPFIFATGYGTQGLPPEYCNRPSVQKPFQMDGLAAVIRSTLKGSAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS02</accession>
    <name>Q3SS02_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Permease YjgP/YjgQ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_1678</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314938.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1678"/>
    <dbReference type="EnsemblBacteria" id="ABA04939">
        <property type="protein sequence ID" value="ABA04939"/>
        <property type="gene ID" value="Nwi_1678"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1678"/>
    <dbReference type="eggNOG" id="COG0795">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028799_2_0_5"/>
    <dbReference type="OMA" id="MTCYLAL"/>
    <dbReference type="OrthoDB" id="1558251at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030923">
        <property type="entry name" value="LptG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005495">
        <property type="entry name" value="LptG/LptF_permease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33529">
        <property type="entry name" value="PTHR33529"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03739">
        <property type="entry name" value="LptF_LptG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04408">
        <property type="entry name" value="LptG_lptG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="281"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="337"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="365" mass="39830" checksum="A96581C3C4E7948F" modified="2005-10-11" version="1">MSMLTNTLGRYFAGRFMVAALGVFASIFLLLVLVDYIEMVRRTSGLASASALMVAETSLFRVPQLLEKLTPFCVLIGAMTCYLALSRKLELVVARAAGVSAWQFISPTLACAIALGVFATTVYNPLSSNLRELSKRMEAEMFGGASGGGLHDASGFWINQVTDDGQAIINAALSEQQGVRLTGLTVFRFDPDSRFRERIEAREAALEDGRWLFKGVRRFTLDNPPVEQETFVLPTSLTPAQVQNSFSTPETVSFWQLPDYIRSSESSGFATAGYRLQYHKLIAQPFLLAAMVMLAASVSLRFFRFGGVQKMVLSGVGSGFLLYVLSKVTEDLSKAELMNPIAAAWLPVCVGGLTGFLVLLHQEDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS43</accession>
    <name>Q3SS43_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1637</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04898">
        <property type="protein sequence ID" value="ABA04898"/>
        <property type="gene ID" value="Nwi_1637"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1637"/>
    <dbReference type="HOGENOM" id="CLU_1439688_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="188" mass="20977" checksum="E63E200132AC6C7A" modified="2005-10-11" version="1">MLRLPRVVLRVRCGDGCLLRKLIEARVQLSYVRLKSIYGRLEGLGILRPGDLHLERVEIRRGESAQLGLYSRELGLKTFDLCIELRSINGWRSLHLGFKEKQAIFKSGGSVCVGRGLMRRGDCRLRFARKPAGIIPYQGHAAEKHHAGNGYAQDQIGAVAFTHHGASLALLYVAYVRRVASLRTGCKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS44</accession>
    <name>Q3SS44_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1636</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04897">
        <property type="protein sequence ID" value="ABA04897"/>
        <property type="gene ID" value="Nwi_1636"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1636"/>
    <dbReference type="HOGENOM" id="CLU_2356887_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="96" mass="10796" checksum="D6115DA94F7874DB" modified="2005-10-11" version="1">MTRGWNPDRVKKTRQNKIWSFASDSIGSEALAAAQTVQYPSPNDKQNNADKHECEKNRNHEASLNQEAVDSRLRPLPYKLGAARSRREGIAFGKSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS87</accession>
    <name>Q3SS87_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Exopolyphosphatase</fullName>
            <ecNumber evidence="3">3.6.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_1593</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[phosphate](n) + H2O = [phosphate](n-1) + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21528"/>
            <dbReference type="Rhea" id="RHEA-COMP:9859"/>
            <dbReference type="Rhea" id="RHEA-COMP:14279"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16838"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="EC" id="3.6.1.11"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GppA/Ppx family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.11" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1593"/>
    <dbReference type="EnsemblBacteria" id="ABA04854">
        <property type="protein sequence ID" value="ABA04854"/>
        <property type="gene ID" value="Nwi_1593"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1593"/>
    <dbReference type="eggNOG" id="COG0248">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025908_4_2_5"/>
    <dbReference type="OMA" id="RISEGCY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004309">
        <property type="term" value="F:exopolyphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006793">
        <property type="term" value="P:phosphorus metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022371">
        <property type="entry name" value="Exopolyphosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003695">
        <property type="entry name" value="Ppx_GppA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02541">
        <property type="entry name" value="Ppx-GppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03706">
        <property type="entry name" value="exo_poly_only"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ppx-GppA" evidence="5">
        <location>
            <begin position="53"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA04854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="529" mass="57634" checksum="D60EF93377461592" modified="2005-10-11" version="1">MESRSRNLRRAVSRVAQSGSRRHRPPAPTYLRPRKPGSSVAVIDIGSNSVRLVVYEALERSLVSVFNEKASCGLGREVQSTGLLASDAISKALTSLRRFHALCKVMKISRVYAIATAACRDATNGADFIAQAERICGVTVEVLPGSQEAYLAALGVVSSVHKPNGIVGDLGGGSLELTDVRDHRVGRGITLPLGSLALQDASKKSLKRANSIVKAALADATPLKAGKSRTFYAIGGTWRALARLHIIQSGYPLRVMHGYSIPAADALDFVRRLRRLVASDMLADIEVVAEARQPLLTYAALVLEHIIRIAKPKAIVFSTYGVREGLLYSMLPAADQSRDGLICAAQTLNGLLSRSACHAEELIKWTDRLFRALKLKETEDDRRLRHTACLLSDIGWRIHPDYRSEQTLSLIMNGNFVSIDHQGRAFVALSVYYRYAGLNEANGPSPEVCELVTPPMDERARILGAAFRVAHLISAAQPGVLSATHFKNSGRKLTLVFEHRLVDLVADRVGGRFKQLARLVGRSGSIVLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSA9</accession>
    <name>Q3SSA9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">Vitamin B12-dependent ribonucleotide reductase</fullName>
            <ecNumber evidence="4 10">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Nwi_1571</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>[thioredoxin]-disulfide + a 2'-deoxyribonucleoside 5'-diphosphate + H2O = [thioredoxin]-dithiol + a ribonucleoside 5'-diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23252"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="EC" id="1.17.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 10">
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the ribonucleoside diphosphate reductase class-2 family.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="4 10"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314838.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1571"/>
    <dbReference type="EnsemblBacteria" id="ABA04832">
        <property type="protein sequence ID" value="ABA04832"/>
        <property type="gene ID" value="Nwi_1571"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1571"/>
    <dbReference type="eggNOG" id="COG0209">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000404_0_1_5"/>
    <dbReference type="OMA" id="WACADPG"/>
    <dbReference type="OrthoDB" id="357568at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02888">
        <property type="entry name" value="RNR_II_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013678">
        <property type="entry name" value="RNR_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000788">
        <property type="entry name" value="RNR_lg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013344">
        <property type="entry name" value="RNR_NrdJ/NrdZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029072">
        <property type="entry name" value="YebC-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08471">
        <property type="entry name" value="Ribonuc_red_2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02867">
        <property type="entry name" value="Ribonuc_red_lgC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01183">
        <property type="entry name" value="RIBORDTASEM1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75625">
        <property type="entry name" value="SSF75625"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02504">
        <property type="entry name" value="NrdJ_Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0846">Cobalamin</keyword>
    <keyword evidence="8 10" id="KW-0170">Cobalt</keyword>
    <keyword evidence="7 10" id="KW-0237">DNA synthesis</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribonuc_red_2_N" evidence="13">
        <location>
            <begin position="31"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="RIBORED_LARGE" evidence="12">
        <location>
            <begin position="198"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="domain" description="RIBORED_LARGE" evidence="12">
        <location>
            <begin position="818"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1133"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1193"/>
            <end position="1222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABA04832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1265" mass="138481" checksum="09C4B4488EF51F4A" modified="2005-10-11" version="1">MRFERRYTTDGQSPYAGIDFRLTTSEIRNPDGSVVFRLENVEVPEFWSQVASDVLAQKYFRKAGVAARLKKVEEETVPSWLWRSVPDTDALAALPESERIVGETSAKQVFDRLAGCWTYWGWKGGYFTSEDDARAFHDELRCMLARQMVAPNSPQWFNTGLHWAYGIDGPGQGHYYVDFKTGKLTRSKSSYEHPQPHACFIQGIEDDLVNEGGIMDLWVREARLFKYGSGTGSNFSRLRGEGERLSGGGRSSGLMSFLKIGDRAAGAIKSGGTTRRAAKMVVVDADHPDIEQYIDWKVKEEQKVAALVTGSRINQKHLKAVMRACVNCEGSGDDCFLPEKNPALRREIKLARRAQVPDNYIKRVIQFARQGYKEIDFPVYDTDWDSEAYLTVSGQNSNNSVSLKDDFLRAVETDGDWNLIGRTNRKVTKTLKARELWEKIGYAAWASADPGLHFNTTMNDWHTCKASGDIRASNPCSEYMFLDDTACNLASANLITFYDTASKQFDVEAYEHTVRLWTVVLEISVLMAQFPSKAIAALSYEFRTLGLGFANIGGLLMTMGLSYDSREGRALCGALSAIMTGIAYRTSAEMAGELGAFPGHKKNAGHMLRVIRNHRRAAHGQAHGYEALAVNPVPLDHASCPQADLIAHATRAWDDALALGEQHGYRNAQVTVVAPTGTIGLVMDCDTTGIEPDFALVKFKKLAGGGYWKIINRAVPEALRALGYRESEIAEIEAYAVGHGLLSNAPGINVSTLKAKGFTDEAIAKVEKALPTAFDIKFAFNKWTFGEDFIRDTLGVDADALASPAFDLLAAIGFSRREIEAANVHICGAMTVEGAPHLRAEHYPVFDCANPCGKIGKRYLSVESHIRMMAAAQPFISGAISKTINMPNDATVDDCKSAYLLSWKLALKANALYRDGSKLSQPLNSQLIVDDDSDDDAIEALMDKPMAARTAQVAEKVVEKLVERIVVLRERERMPDRRKGYTQKAVVGGHKVYVRTGEYDDGRLGEIFIDMHKEGAALRSFINNFAIAVSLGLQYGVPLEEYVDAFTFTRFEPAGPVQGNDSIKYATSILDYVFRELAVSYMGRYDLAHVDPSETGFDALGKGVAEGKTPEGQQATRYVSKGLTRSRTDNLVVMRGGNDARGSSSSAAGDGGARTSVPSPLAGEGRAAAASVTALSSSRAGDTLEGAAALKQETGHDLSPTERLEQQQRNKVAAPAIAPTKAERRAEAKAKGYEGEMCPECANFTLVRNGTCMKCDTCGSTTGCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSB4</accession>
    <name>Q3SSB4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Single-stranded DNA-binding protein</fullName>
            <shortName evidence="3">SSB</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_1566</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Plays an important role in DNA replication, recombination and repair. Binds to ssDNA and to an array of partner proteins to recruit them to their sites of action during DNA metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314833.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1566"/>
    <dbReference type="EnsemblBacteria" id="ABA04827">
        <property type="protein sequence ID" value="ABA04827"/>
        <property type="gene ID" value="Nwi_1566"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1566"/>
    <dbReference type="eggNOG" id="COG0629">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078758_0_1_5"/>
    <dbReference type="OMA" id="GQMQERT"/>
    <dbReference type="OrthoDB" id="1942216at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04496">
        <property type="entry name" value="SSB_OBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00984">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000424">
        <property type="entry name" value="Primosome_PriB/ssb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011344">
        <property type="entry name" value="ssDNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10302">
        <property type="entry name" value="PTHR10302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00436">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00621">
        <property type="entry name" value="ssb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50935">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="2 3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="1 3 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="DNA-binding region" evidence="3">
        <location>
            <begin position="54"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="118"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Important for interaction with partner proteins" evidence="3">
        <location>
            <begin position="158"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="163" mass="17729" checksum="066CA755E972FE3E" modified="2005-10-11" version="1">MAGSVNKVILIGNLGADPEIKRTQDGRPIANLRIATSESWRDRTSGERKERTEWHRVVIFNEGLCKVVEQYLKKGSKVYVEGQLQTRKWTDQNGVEKYSTEVVLQNFNSTLTMLDGRNSGGGGNFGPDDAGSGFGSGSPSGAAPRRAAPASSHRGDMDDDIPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSC6</accession>
    <name>Q3SSC6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1554</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MT-A70-like family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314821.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1554"/>
    <dbReference type="EnsemblBacteria" id="ABA04815">
        <property type="protein sequence ID" value="ABA04815"/>
        <property type="gene ID" value="Nwi_1554"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1554"/>
    <dbReference type="eggNOG" id="COG4725">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_370820_0_0_5"/>
    <dbReference type="OrthoDB" id="1487464at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044260">
        <property type="term" value="P:cellular macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043412">
        <property type="term" value="P:macromolecule modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090304">
        <property type="term" value="P:nucleic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007757">
        <property type="entry name" value="MT-A70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05063">
        <property type="entry name" value="MT-A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51143">
        <property type="entry name" value="MT_A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="311"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="367"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="430"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="372"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="434"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="750" mass="81912" checksum="C7C35886FB77BF09" modified="2005-10-11" version="1">MTELVKYEEARRLLAEAVAVDEVKLIRDQAVALAAYARQAKDREIEVAAAQIRFRAEQRLGEMIIAQKETVGLAKGGQPYRNPTCSDKEQVGTPVPTLEQAGIDRKLSSRAQRMARLPEDKFEELLERHADEVRSGVRVTMDLLKVGEEADGRERRRALQQALSDETARLPTGRRFPVIYADPPWHRKQGVTGRSYENHYPTMTWDDICALPVKDLLLPDAWVFLWIPRAHMFALHAVDMEFATDDGCVITRKVQLPLAWAVARAWGCDSYSTAFVWTKTDEEHPEDQGGGVITFDQDEILLLFKRGRGLPKPDTKEKFGSNHRERSKPLGHSRKPEFYREMIATMCGGLPVMELFARVDAEHPLPPKWEGWGNQTSPSSGDNEIAETVAPGSTADESTAGNATMLPVAAPTLSADDGLDLPACLRRTKSEVPSGASDGGSENTSNLSVSGDADREQQRDTLGVTAGRDRQPTSPMSELVEWKVLGAIDANVSISGPIDGLIESGLVDFDFDKLLFLTDEGKERFAVLEQKFNPAPESDSFVGSADAVDEIADEVVGIADAVDVGEPEMAGASAGPLPSTPDSGPDEFTILKAFANSATRDRFNIIGPVASDYIARGLVFKYDTITDWGLTAAGYDRLDELKRERAGLAPKVLTPPAVSDLSDDEQEMREVLAAIAGAYRSDLSATRRATYERDLIGLGFLSVIDDCGGLAVTEAGHAWLRETEAGSAPPPLTQPEPLTPVQINMFGAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSC9</accession>
    <name>Q3SSC9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1551</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314818.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1551"/>
    <dbReference type="EnsemblBacteria" id="ABA04812">
        <property type="protein sequence ID" value="ABA04812"/>
        <property type="gene ID" value="Nwi_1551"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1551"/>
    <dbReference type="eggNOG" id="ENOG50331AT">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051256_2_2_5"/>
    <dbReference type="OMA" id="QPWAWLI"/>
    <dbReference type="OrthoDB" id="2016960at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="130" mass="14384" checksum="E43DCF1F6C081381" modified="2005-10-11" version="1">MKAISIRQPWAWAILHAGKDIENRDRRWNLRGRIVVHASATMTRAEMLAIDDIASFGLRPPTVADFRACGLLGALVGTVEIVDCVRDHPSLWFCGPFGLVLREPRPFKTPIPYKGALGVMDVPDGILPPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSI4</accession>
    <name>Q3SSI4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Phage integrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_1496</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314763.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1496"/>
    <dbReference type="EnsemblBacteria" id="ABA04757">
        <property type="protein sequence ID" value="ABA04757"/>
        <property type="gene ID" value="Nwi_1496"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1496"/>
    <dbReference type="eggNOG" id="COG0582">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027562_0_2_5"/>
    <dbReference type="OMA" id="AYNRSNY"/>
    <dbReference type="OrthoDB" id="785253at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="6">
        <location>
            <begin position="30"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="5">
        <location>
            <begin position="142"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="343" mass="38387" checksum="8B499C83F530DACA" modified="2005-10-11" version="1">MIDGLDPIEDRNARKLAFVAERETQKANATTFRQCAEAYIKAHQAGWKSAKHGSQWAATLERYAYPVIGNVPVALVDRNHIMKILEPIWTTKTETASRVRGRIEKVLDRARAIGLREAENPARWVGHLDQLLPKKSQVAPVEHFAALPYKQLPAFMAKLRKRDGISARALEFTILNVTRTANTIGAKWEQINESEQTWTIPGELMKGQKGRRRPDHVIPLSDRALSILQDLPREGSFIFPGARGEGLSNMAMAETLKEMGYSGDVATVHGFRSTFKDWASEQTSYANELTEMAMAHIVSSKTERAYRRGDQREKRVRLMADWAGYCESKATGGDNVVAIGAGR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSU5</accession>
    <name>KAD_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Adenylate kinase</fullName>
            <shortName evidence="1">AK</shortName>
            <ecNumber evidence="1">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP-AMP transphosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">ATP:AMP phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Adenylate monophosphate kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">adk</name>
        <name type="ordered locus">Nwi_1385</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from ADP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the adenylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314659.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSU5"/>
    <dbReference type="SMR" id="Q3SSU5"/>
    <dbReference type="STRING" id="323098.Nwi_1385"/>
    <dbReference type="EnsemblBacteria" id="ABA04646">
        <property type="protein sequence ID" value="ABA04646"/>
        <property type="gene ID" value="Nwi_1385"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1385"/>
    <dbReference type="eggNOG" id="COG0563">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032354_1_2_5"/>
    <dbReference type="OMA" id="FHNRMRV"/>
    <dbReference type="OrthoDB" id="1491686at2"/>
    <dbReference type="UniPathway" id="UPA00588">
        <property type="reaction ID" value="UER00649"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:adenylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044209">
        <property type="term" value="P:AMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006259">
        <property type="entry name" value="Adenyl_kin_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="PTHR23359"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01351">
        <property type="entry name" value="adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000058866" description="Adenylate kinase">
        <location>
            <begin position="1"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="NMP" evidence="1">
        <location>
            <begin position="30"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="1">
        <location>
            <begin position="126"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="57"/>
            <end position="59"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="85"/>
            <end position="88"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00235"/>
        </source>
    </evidence>
    <sequence length="206" mass="22567" checksum="93C93489694CB970" modified="2005-10-11" version="1">MRLILLGPPGAGKGTQAQRLVHHYGIIQLSTGDMLRAAVAAGTPVGLKAKDIMASGGLVPDDVVIGIISDRLEQADAKDGFILDGFPRTVPQAEALDILLQSKNLALDAVIELRVDESALLQRVENRVAETTARGEQVRADDNPEVLSQRLASYRALTEPLIRYYSERGRLLTVDGMMAIDDVTRDIRRVLRRSGVPMSKFGVRRW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSV4</accession>
    <name>RL5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplE</name>
        <name type="ordered locus">Nwi_1376</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits; this bridge is implicated in subunit movement. Contacts the P site tRNA; the 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome-bound tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit; part of the 5S rRNA/L5/L18/L25 subcomplex. Contacts the 5S rRNA and the P site tRNA. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314650.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSV4"/>
    <dbReference type="SMR" id="Q3SSV4"/>
    <dbReference type="STRING" id="323098.Nwi_1376"/>
    <dbReference type="EnsemblBacteria" id="ABA04637">
        <property type="protein sequence ID" value="ABA04637"/>
        <property type="gene ID" value="Nwi_1376"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1376"/>
    <dbReference type="eggNOG" id="COG0094">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061015_2_1_5"/>
    <dbReference type="OMA" id="ERMYAFL"/>
    <dbReference type="OrthoDB" id="1456375at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01333_B">
        <property type="entry name" value="Ribosomal_L5_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002132">
        <property type="entry name" value="Ribosomal_L5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020930">
        <property type="entry name" value="Ribosomal_L5_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031309">
        <property type="entry name" value="Ribosomal_L5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020929">
        <property type="entry name" value="Ribosomal_L5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_L5_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031310">
        <property type="entry name" value="Ribosomal_L5_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11994">
        <property type="entry name" value="PTHR11994"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00281">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00673">
        <property type="entry name" value="Ribosomal_L5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002161">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="SSF55282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00358">
        <property type="entry name" value="RIBOSOMAL_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000243029" description="50S ribosomal protein L5">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01333"/>
        </source>
    </evidence>
    <sequence length="185" mass="20886" checksum="8289ADEC115C91B1" modified="2005-10-11" version="1">MAETAYVPRLRAEYDKSIRSRLTEQFGYVNVMQVPRLDKVVLNMGIGEAVNDRKKAEAAAGDLSLIAGQKAVVTYSRVAISTFKLRENQPIGCKVTLRKTKMYEFIDRLVNVALPRVRDFRGLNPKSFDGRGNYSLGIKEHIIFPEIDFDKVGESWGMDVTVCTTAQTDDEARALLTAFNFPFRQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSV5</accession>
    <name>RL24_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplX</name>
        <name type="ordered locus">Nwi_1375</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of two assembly initiator proteins, it binds directly to the 5'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="1">One of the proteins that surrounds the polypeptide exit tunnel on the outside of the subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314649.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSV5"/>
    <dbReference type="SMR" id="Q3SSV5"/>
    <dbReference type="STRING" id="323098.Nwi_1375"/>
    <dbReference type="EnsemblBacteria" id="ABA04636">
        <property type="protein sequence ID" value="ABA04636"/>
        <property type="gene ID" value="Nwi_1375"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1375"/>
    <dbReference type="eggNOG" id="COG0198">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093315_2_2_5"/>
    <dbReference type="OMA" id="HVKPTQE"/>
    <dbReference type="OrthoDB" id="2040741at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06089">
        <property type="entry name" value="KOW_RPL26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01326_B">
        <property type="entry name" value="Ribosomal_L24_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041988">
        <property type="entry name" value="KOW_RPL26/RPL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003256">
        <property type="entry name" value="Ribosomal_L24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005825">
        <property type="entry name" value="Ribosomal_L24/26_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12903">
        <property type="entry name" value="PTHR12903"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17136">
        <property type="entry name" value="ribosomal_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01079">
        <property type="entry name" value="rplX_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01108">
        <property type="entry name" value="RIBOSOMAL_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000241627" description="50S ribosomal protein L24">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01326"/>
        </source>
    </evidence>
    <sequence length="104" mass="11189" checksum="CEB608C2055AA628" modified="2005-10-11" version="1">MAAKIRKGDKVIVLSGRDKGRTGEVFEVRPAEGRALVRGVNIVKRHQKQTQNQEGGILSKESPIDLSNIAIVGKDGKPTRVGFKIQADGTKVRVAKRSGAEIDG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSW1</accession>
    <name>RL22_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplV</name>
        <name type="ordered locus">Nwi_1369</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein binds specifically to 23S rRNA; its binding is stimulated by other ribosomal proteins, e.g. L4, L17, and L20. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="1">The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009797133.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSW1"/>
    <dbReference type="SMR" id="Q3SSW1"/>
    <dbReference type="STRING" id="323098.Nwi_1369"/>
    <dbReference type="EnsemblBacteria" id="ABA04630">
        <property type="protein sequence ID" value="ABA04630"/>
        <property type="gene ID" value="Nwi_1369"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1369"/>
    <dbReference type="eggNOG" id="COG0091">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083987_3_0_5"/>
    <dbReference type="OMA" id="KRIQPRA"/>
    <dbReference type="OrthoDB" id="1666043at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00336">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01331_B">
        <property type="entry name" value="Ribosomal_L22_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_L22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036394">
        <property type="entry name" value="Ribosomal_L22/L17_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005727">
        <property type="entry name" value="Ribosomal_L22_bac/chlpt-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13501">
        <property type="entry name" value="PTHR13501"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54843">
        <property type="entry name" value="SSF54843"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01044">
        <property type="entry name" value="rplV_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000243175" description="50S ribosomal protein L22">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01331"/>
        </source>
    </evidence>
    <sequence length="128" mass="14159" checksum="8C0A2FF6D36EC4DF" modified="2005-10-11" version="1">MSKPKRERSLPENEAKAIARMLRVSPQKLNLVAQLIRGRKASAALADLQFSRKRIAGDVKKCLESAIANAENNHDLDVDELIVSEAFVGNGMVMKRFAPRGRGRSGRIYKPFSQLTIVVRQVEAEASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSX8</accession>
    <name>Q3SSX8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1352</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1352"/>
    <dbReference type="EnsemblBacteria" id="ABA04613">
        <property type="protein sequence ID" value="ABA04613"/>
        <property type="gene ID" value="Nwi_1352"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1352"/>
    <dbReference type="eggNOG" id="COG0654">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1676038_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="157" mass="17580" checksum="B3C6316249D21E39" modified="2005-10-11" version="1">MAYLTLFPIGNTMRANLMVYRSMNDIWFHEFRENPEAAMSAMMPGLDRITGGFKVSGQIKIRPADLYVTENHRQAGVVVIGDAFATSCPAAGTGTDKVFTDVERLCNHHIPHWLATEGMDRAKIKMFYDDPVKMECDAWSAAKAWHLRSLSLDNGPT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSX9</accession>
    <name>RPOC_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit beta'</fullName>
            <shortName evidence="1">RNAP subunit beta'</shortName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">RNA polymerase subunit beta'</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Transcriptase subunit beta'</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpoC</name>
        <name type="ordered locus">Nwi_1351</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314630.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSX9"/>
    <dbReference type="SMR" id="Q3SSX9"/>
    <dbReference type="STRING" id="323098.Nwi_1351"/>
    <dbReference type="EnsemblBacteria" id="ABA04612">
        <property type="protein sequence ID" value="ABA04612"/>
        <property type="gene ID" value="Nwi_1351"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1351"/>
    <dbReference type="eggNOG" id="COG0086">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000524_3_1_5"/>
    <dbReference type="OMA" id="YRNIRVE"/>
    <dbReference type="OrthoDB" id="4421at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01322">
        <property type="entry name" value="RNApol_bact_RpoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012754">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042102">
        <property type="entry name" value="RNA_pol_Rpb1_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="PTHR19376"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02386">
        <property type="entry name" value="rpoC_TIGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000225556" description="DNA-directed RNA polymerase subunit beta'">
        <location>
            <begin position="1"/>
            <end position="1399"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="462"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="464"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="466"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="810"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="884"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="891"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="894"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01322"/>
        </source>
    </evidence>
    <sequence length="1399" mass="155812" checksum="A2F5765E49F6BCB4" modified="2005-10-11" version="1">MNQEVMNLFNPTTPAQVFDQIRISIASPEKILSWSYGEIKKPETINYRTFKPERDGLFCARIFGPIKDYECLCGKYKRMKYKGIICEKCSVEVTLSRVRRERMGHIELAAPVAHIWFLKSLPSRIGLLLDMTLKDLERILYFEYYVVLEPGLTALKDRQLLSEEEYLRAQDEYGQDSFTAMIGAEAIRELLKGLELERLEAELRAEMQATESDIKHKKLAKRLKIVEAFRFSGNKPEWMILTVVPVIPPDLRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLMELRAPDIIIRNEKRMLQEAVDALFDNGRRGRVITGANKRPLKSLADMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELKLHQCGLPKKMALELFKPFIYSRLDAKGLSTTVKQAKKLVEKERPEVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPTLIEGKAIQLHPLVCAAFNADFDGDQMAVHVPLSLEAQLEARVLMMSTNNILHPANGQPIIVPSQDIVLGLYYLSIMREGLPGEGKVFGDMAELEHALHSGVIHLHTSIKYRWDQTDENGEPIKRLIDTTAGRVMLGQVLPKSQKISFDAINKLMTKREISGVIDQVYRHCGQKETVIFCDRIMALGFYNAFKAGISFGKDDMVVPHGKWKIVDTTRALAKDFEQQYNDGLITHGEKYNKVVDAWSKATEEIAKEMMKEISSTKKDAKGIEAQINSIYMMAHSGARGSPAQMRQLAGMRGLMAKPSGEIIETPIISNFKEGLSVLEYFNSTHGARKGLADTALKTANSGYLTRRLVDVAQDCIITQDDCGTKLGIKMRAIVDAGTVVASLGSRILGRTACEDIRNPASNEVLIERNTLMEESHVDQISKAGIQEVKIRSALTCELINGICGKCYGRDLARGTPVNHGEAVGVIAAQSIGEPGTQLTMRTFHIGGAAQINEQSVIESNFDGKITIRNKAIARNGEGHLVAMVRNMVVAVVDADGTERATHRIQYGARMHVDEGDMVKRGQRIAEWDPYTRPVLTELEGTIGFEDLIEGQSISETLDESTGIAKRIVIDWRGTRGGADLRPAIVIKGKDGKVLKLPRGGDARYMLSVDAILSVDVGAKVKPGDILARISTESAKTRDITGGLPRVAELFEARRPKDAAIIAEIAGTIRFGRDYKNKRRISIEPVDQEEETREYLIPKGKHIHLQDGDIVEKGDFIVDGNPAPHDILAIKGIEELAAYLVNEIQEVYRLQGVLINDKHIEVIVRQMLQKVEIIDQGETDMISGEQIDKIEFDRLNLKAQEEGKKIATGTPVLLGITKASLQTRSFFSAASFQETTRVLTEAAVNGKVDPLEGLKENVIVGRLIPAGTGASMAKIREVAVKRDRLILDEREKQAAIVPSAPEAEPLALPPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSY8</accession>
    <name>Q3SSY8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1342</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04603">
        <property type="protein sequence ID" value="ABA04603"/>
        <property type="gene ID" value="Nwi_1342"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1342"/>
    <dbReference type="HOGENOM" id="CLU_2586157_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="80" mass="8870" checksum="612CF23A1297A1DC" modified="2005-10-11" version="1">MSLSLTASHSILDVGSHPKDRLGGVFWPTRQSLIHQHQDHLHVLPQQGRVVAVLRRSSQRPDVLQGFDGIDGRLFIPLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST23</accession>
    <name>Q3ST23_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1307</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1307"/>
    <dbReference type="EnsemblBacteria" id="ABA04568">
        <property type="protein sequence ID" value="ABA04568"/>
        <property type="gene ID" value="Nwi_1307"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1307"/>
    <dbReference type="eggNOG" id="ENOG50331C8">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1276650_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="225" mass="25784" checksum="4B0CDD7FA9446EE4" modified="2005-10-11" version="1">MKQIDEVDIFKFWKEVGPSEKVHPADREILFNAPNKFDLDYLPTPFYGPLRTAPIVLLYTNPGLSDKDKKAGSDPSEQQFYFRQRQGNEWLRDQRGLTEKSWWVSRTKRICADPEYLRSRVAVLELCPYHSSSFVDAKMARTLPSYQTALGWAHQYLFPMARARERVVICLRAAKRWGLQRNASDGFLFAPGVNRSGHMLSQDRDVIIQTARKIIGLDSGSGACL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST80</accession>
    <name>Q3ST80_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Resolvase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_1250</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
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            <taxon>Proteobacteria</taxon>
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            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ABA04511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1250"/>
    <dbReference type="EnsemblBacteria" id="ABA04511">
        <property type="protein sequence ID" value="ABA04511"/>
        <property type="gene ID" value="Nwi_1250"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1250"/>
    <dbReference type="eggNOG" id="COG1961">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010686_18_17_5"/>
    <dbReference type="OMA" id="KYQNRYP"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038109">
        <property type="entry name" value="DNA_bind_recomb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011109">
        <property type="entry name" value="DNA_bind_recombinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006118">
        <property type="entry name" value="Recombinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025827">
        <property type="entry name" value="Zn_ribbon_recom_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07508">
        <property type="entry name" value="Recombinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13408">
        <property type="entry name" value="Zn_ribbon_recom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="SSF53041"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00397">
        <property type="entry name" value="RECOMBINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="7">
        <location>
            <begin position="17"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Recombinase" evidence="8">
        <location>
            <begin position="170"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="369"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-serine intermediate" evidence="4 5">
        <location>
            <position position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606118-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA04511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="524" mass="59722" checksum="F89AC9705CCD8A47" modified="2005-10-11" version="1">MRKIAKPAPAADSSRKQAVIYARVSSKEQEKEGYSIPAQLKLLKDYAAAEGFMVAQDYVDVETAKQTGRAAFGKMVAWLRAHPDVRAIIVEKTDRLYRNLKDWVTIDELDIEIHFPKEGVVLSRESRSSEKFMHGIKVLMAKNYIDNLSEEARKGQQEKAEQGIWPTKTPLGYRNVTGADGRKVIAVDPQVAPIVAKLFEWYATGEFALKEVAKKARAAGLVYKKSGAPVPTSTVHSILRNRLYTGWFEWNGKLYQGKHEPLVSVEAWERVQGVMDGRNASKHRRMTHDFAFSGLIACGRCGCSVVGEIKKQKYIYYHCTGYADKCRDKPASCRRKYVREEVLEQQFTAMLGRLRFDDEVLAWVREALHASHADERREHEEAIKRLQAEHKRLGERISAMYIDKLDGKIGGDFYDRFAGQWREEQQRLQRDIGRHEQAEQSYMDEGVRILELARNAQALFERQPAREKRRLLNFVLSNCSWEDGEVVATFRQPFDLLAETTAIAARSSAGNGDNSSKSEIWLGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST83</accession>
    <name>Q3ST83_NITWN</name>
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        <submittedName>
            <fullName evidence="5">Acyl carrier protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_1246</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1246"/>
    <dbReference type="EnsemblBacteria" id="ABA04508">
        <property type="protein sequence ID" value="ABA04508"/>
        <property type="gene ID" value="Nwi_1246"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1246"/>
    <dbReference type="eggNOG" id="COG0236">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2234373_0_0_5"/>
    <dbReference type="OMA" id="ATEEYFV"/>
    <dbReference type="OrthoDB" id="1943389at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="SSF47336"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="4">
        <location>
            <begin position="2"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="3">
        <location>
            <position position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="93" mass="10275" checksum="C3AB9F733008841D" modified="2005-10-11" version="1">MSSTFDRVATIIAETCDIPRDTITPESHAIDDLGIDSLDFLDIAFAIDKAFGIKLPLEKWTQEVNDGKATTEQYFVLKNLSARIDELVAAKDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STB3</accession>
    <name>Q3STB3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Extracytoplasmic solute receptor protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">TRAP transporter</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_1216</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Part of the tripartite ATP-independent periplasmic (TRAP) transport system.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the bacterial solute-binding protein 7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314507.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1216"/>
    <dbReference type="EnsemblBacteria" id="ABA04478">
        <property type="protein sequence ID" value="ABA04478"/>
        <property type="gene ID" value="Nwi_1216"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1216"/>
    <dbReference type="eggNOG" id="COG4663">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036176_0_1_5"/>
    <dbReference type="OMA" id="FWEGGPT"/>
    <dbReference type="OrthoDB" id="1251304at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031317">
        <property type="term" value="C:tripartite ATP-independent periplasmic transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043177">
        <property type="term" value="F:organic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015849">
        <property type="term" value="P:organic acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13682">
        <property type="entry name" value="PBP2_TRAP_alpha-ketoacid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018389">
        <property type="entry name" value="DctP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026289">
        <property type="entry name" value="SBP_TakP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041722">
        <property type="entry name" value="TakP/all3028"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019546">
        <property type="entry name" value="TAT_signal_bac_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038404">
        <property type="entry name" value="TRAP_DctP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33376">
        <property type="entry name" value="PTHR33376"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03480">
        <property type="entry name" value="DctP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10518">
        <property type="entry name" value="TAT_signal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039026">
        <property type="entry name" value="SiaP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01409">
        <property type="entry name" value="TAT_signal_seq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0574">Periplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229299" description="Extracytoplasmic solute receptor protein" evidence="6">
        <location>
            <begin position="26"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4 5">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>a metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:25213"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>a metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:25213"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR039026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR039026-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR039026-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="362" mass="39856" checksum="3121ECC32F780F55" modified="2005-10-11" version="1">MKRRDFLKGSAAGAAVSAVASPAIAQSAPEIKWRMTSSFPKSLDTIYGTAAVFAKQVAEMTDNKFQIQLFAAGEVVPALQALDATANNTVEMSHTASYYYVGKDPTFAIFASVPFGLNARQQNSWLFQGGGNELADEFFKKFGVVGLPCGNTGTQMGGWFRKEIKTVADLSGLKMRIGGIAGQVLQKVGVIPQQLAGGDVYPSLEKGTIDAAEWVGPYDDEKLGLQKVAKYYYYPGFWEGGPTLHAFCNLEKWNTLPKTYQAILVNAANTANTWMTARYDMQNPTALKRLVAGGTQLRPFSHDVLDACLKATNELWGEISANNADFKKVIDAMQAYRSDEYLWWQVAEYTYDTFMIRSRTRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STD2</accession>
    <name>Q3STD2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">COX4_pro_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1197</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04459">
        <property type="protein sequence ID" value="ABA04459"/>
        <property type="gene ID" value="Nwi_1197"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1197"/>
    <dbReference type="HOGENOM" id="CLU_174805_1_0_5"/>
    <dbReference type="OMA" id="RTYNAFV"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036596">
        <property type="entry name" value="Cyt-C_aa3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012422">
        <property type="entry name" value="Cyt_c_oxidase_su4_bac-aa3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07835">
        <property type="entry name" value="COX4_pro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81469">
        <property type="entry name" value="SSF81469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="COX4_pro_2" evidence="2">
        <location>
            <begin position="4"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="50" mass="5402" checksum="D4CB6274B36C28E0" modified="2005-10-11" version="1">MSDHSGPVYSAAEGMDYREHERTYNAFVRLGTILASTAAAVIALLAIFLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STD6</accession>
    <name>Q3STD6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cytochrome c-type biogenesis protein CcmF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_1193</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CcmF/CycK/Ccl1/NrfE/CcsA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314484.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1193"/>
    <dbReference type="EnsemblBacteria" id="ABA04455">
        <property type="protein sequence ID" value="ABA04455"/>
        <property type="gene ID" value="Nwi_1193"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1193"/>
    <dbReference type="eggNOG" id="COG1138">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015041_3_0_5"/>
    <dbReference type="OMA" id="SYWGMQL"/>
    <dbReference type="OrthoDB" id="213184at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015232">
        <property type="term" value="F:heme transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032523">
        <property type="entry name" value="CcmF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002541">
        <property type="entry name" value="Cyt_c_assembly"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003567">
        <property type="entry name" value="Cyt_c_biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003568">
        <property type="entry name" value="Cyt_c_biogenesis_CcmF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43653">
        <property type="entry name" value="PTHR43653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16327">
        <property type="entry name" value="CcmF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01578">
        <property type="entry name" value="Cytochrom_C_asm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01410">
        <property type="entry name" value="CCBIOGENESIS"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01411">
        <property type="entry name" value="CCMFBIOGNSIS"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00353">
        <property type="entry name" value="nrfE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="96"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="207"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="312"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="352"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="394"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="425"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="449"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="489"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="618"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrom_C_asm" evidence="8">
        <location>
            <begin position="89"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="CcmF_C" evidence="9">
        <location>
            <begin position="315"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA04455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="661" mass="71395" checksum="EA4E37B8DDAD2B2A" modified="2005-10-11" version="1">MIAEAGHFALVLALGLALIQSVVPIIGARLHDGALMNVARSTALAQLLFVAASFFALATLYVVSDFSVLNVFQNSHSLKPMIYKITGVWGNHEGSMLLWVTILALFGGLVAAFGNNLPLTLRAHVLAVQGWVATAFYLFILLTSNPFTRIASPPMEGRDLNPVLQDIGLAVHPPMLYLGYVGFSIAFSFAVAALIEGRIDAAWARWVRPWTLVAWIFLTLGIAMGSYWAYYELGWGGWWFWDPVENASLMPWLAGTALLHSSVVMEKRNALKVWTILLAILTFSLSLLGTFLVRSGVLTSVHAFATDPTRGVFILLILCTFIGGSLTLFIWRASTLKQGGLFAPVSREGALVLNNLFLTTACATVFVGTLYPLALEVITGNRISVGAPFFNLTFAPLFVPLLIAVPFGPLLAWKRGDLLGASQRLIAAGVGSVIAVAVLWAWTRGGSTFAPLAIGLAVFVIIGALCDLAERVNLFRASLPVSLRRAKGLPRSAWGTVFAHAGLGVALIGIVCETTWNTEYIASMKINDVARVGGYSLTFEGTTQHQRSNYREDIARFTVSRDGQPIAVMMPSKRNFTTRAASTTEAALLTRGASQLYISLGEATADGSIAVRIYHKPMVLLIWFGPVLMAFGGVLSLSDRRLRVGAPKPAKAGWRTLQPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STF2</accession>
    <name>Q3STF2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Gene transfer agent (GTA) like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1177</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314468.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1177"/>
    <dbReference type="EnsemblBacteria" id="ABA04439">
        <property type="protein sequence ID" value="ABA04439"/>
        <property type="gene ID" value="Nwi_1177"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1177"/>
    <dbReference type="eggNOG" id="COG5281">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109775_0_0_5"/>
    <dbReference type="OMA" id="RVMPFAR"/>
    <dbReference type="OrthoDB" id="1857960at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="194" mass="19522" checksum="DFDFC3F3A903E702" modified="2005-10-11" version="1">MTDDPSLHETSRTLDDLTVRTQALTTSAGGFARAMTQAFSSSVSGGKQFDDVLKTLALRVSSLAVTAAFKPLTASLTSGISSLFSGLAGSIGSFGGVQAHALGGIKPFAAGGVIGAPSYFPMMDGGVGLAGEAGPEAIMPLSRGPDGRLGVSGKGGGNSITVQIATPDLDSFRRSESYIAGQIARAVARGQRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STF9</accession>
    <name>Q3STF9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF2019 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314461.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04432">
        <property type="protein sequence ID" value="ABA04432"/>
        <property type="gene ID" value="Nwi_1170"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1170"/>
    <dbReference type="eggNOG" id="ENOG5033ZRP">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2410272_0_0_5"/>
    <dbReference type="OrthoDB" id="1764604at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018568">
        <property type="entry name" value="DUF2019"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09450">
        <property type="entry name" value="DUF2019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="SSF48371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2019" evidence="1">
        <location>
            <begin position="12"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="92" mass="10377" checksum="79F62859737349F2" modified="2005-10-11" version="1">MISVQPNALVKNLVAQFVEIGVAQYDAINVIDNAMYNRLYKKMERIRAELRKMPGDQRRALLPLLSHPNVQVRLKAAHSLSCALSRFGPQCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STJ9</accession>
    <name>Q3STJ9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Flagellar biosynthetic protein FliP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">fliP</name>
        <name evidence="13" type="ordered locus">Nwi_1130</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Plays a role in the flagellum-specific transport system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Bacterial flagellum basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the FliP/MopC/SpaP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314423.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1130"/>
    <dbReference type="EnsemblBacteria" id="ABA04392">
        <property type="protein sequence ID" value="ABA04392"/>
        <property type="gene ID" value="Nwi_1130"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1130"/>
    <dbReference type="eggNOG" id="COG1338">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042028_0_0_5"/>
    <dbReference type="OMA" id="MPPNQIL"/>
    <dbReference type="OrthoDB" id="1049349at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044781">
        <property type="term" value="P:bacterial-type flagellum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005837">
        <property type="entry name" value="FliP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005838">
        <property type="entry name" value="T3SS_IM_P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30587">
        <property type="entry name" value="PTHR30587"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30587:SF0">
        <property type="entry name" value="PTHR30587:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00813">
        <property type="entry name" value="FliP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00951">
        <property type="entry name" value="FLGBIOSNFLIP"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01302">
        <property type="entry name" value="TYPE3IMPPROT"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01103">
        <property type="entry name" value="fliP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01060">
        <property type="entry name" value="FLIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01061">
        <property type="entry name" value="FLIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="7 12" id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword evidence="11 12" id="KW-1006">Bacterial flagellum protein export</keyword>
    <keyword evidence="5 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="13" id="KW-0969">Cilium</keyword>
    <keyword evidence="13" id="KW-0282">Flagellum</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="47"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA04392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="251" mass="26950" checksum="10B2A71DD6E50875" modified="2005-10-11" version="1">MSAATHPRRVSFFLILITAGSLTDPALAQDISINLGGAGNGGVTERAIQLIALLTVLSIAPSILIMMTSFTRIVVVLSLLRTALGTATAPPNSVIIALAMFLTAFVMGPTLQKSYDEGIKPLVANETTVEEALQRASVPLRGFMQKNVREQDLKLFMDLSGEPPPATPADLSLRILMPAFMISELKRAFEIGFLLFLPFLIIDLVVASVLMSMGMMMLPPVVVSLPFKLIFFVLVDGWALVAGSLVQSYGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STK2</accession>
    <name>Q3STK2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Tetratricopeptide TPR_4 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1127</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314420.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1127"/>
    <dbReference type="EnsemblBacteria" id="ABA04389">
        <property type="protein sequence ID" value="ABA04389"/>
        <property type="gene ID" value="Nwi_1127"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1127"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004821_0_0_5"/>
    <dbReference type="OMA" id="RRGDEMI"/>
    <dbReference type="OrthoDB" id="781824at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="907"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="299"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1210"/>
            <end position="1247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="385"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1247" mass="135256" checksum="79864BD9C266257E" modified="2005-10-11" version="1">MILSENGFSTFRDHAPGRACARRLRRGFGPVLLTAVLLLAAFPDASRAQAVRGEASFSAADGFARLVLKLNDDVDSEVTVAGAVLVIRFKRPVDIPVGRIAVAVPDYVASARRDPDGSAIRLALARNVTVNTMTAGERVFVDLMPDGWKGPPPGLPQEVVRELAERARAAERALREQRSLEAAKKRPPVRVRASVQPTFVRYVFELPDGAGVSSALNEQKLVLLFRPALTFDLADAKLMAPANVEQIAQKVEGEGSRIDIKLIGDVDVHSFREEKNYIVDIGFQQTGKPVLPAPLASTEARPQVDAAGKAAAETPPPASRVIAERAAQKVKPSTADVKPVPAAKMSEAAPVVAATPAPVPPRIDKPVAAEATPAPEIVPSKEIASEARPADDQKARPTSVKQEPAKAAAASEPATIPALKEKIANDAATLNAERRTEGFRLTLGFGAPAPAALFRRADAVWLLFDSKQPVDINAIRKEGGSLITDIDRRPLDSGQAIRIRLTSPQLASLAPADTDAPTSAAAGRNWILTFADAVQQPSRALSAQRNVVDRAHASVTVPLAGAGRLHRIVDPDSGNALMVVTALPPARGLIRRQQFVEFSLGESIHGVVIEPKSDDIAVDVATDKVTVTRQGGLTLSAVNAAPGRASAAAQPIFDVAQWRKDSQADFLERLDELVDAASTAGDDDARAAARIDLARFYMARGFHHEARAVLDLALSETNPGQQDPVLLIIHSVASSLTNYTASAMRDLATPAIGASYDSELWKALALARQQKWAEAREKFKNAHFAISVLPLDLQRLIVTAAMRASLEVGDFSGVASHSTDLDALGIPDELKPRITVMRARLSEALGREKEALAQYREVMASSDREAATEAQIHDIALRQKRKEIKPEDALRDLETLSVMWRGDRLEVQTLKMLMALYVDAGRYAEALAVSRTATRLEPDSAMSRQIQDDASALFARLFLSTKGDNLPPVDALAMFYDFRDLTPIGRRGDEMIRRLADRLVAFDLLDQAGELLQYQVDHRLEGAARAQVAARLATIYLMNRKPDRAIAALRTTRIADLAGALRQQRLLLEARAESDIGRRDLALDIIFNLDGREAIRLRSDIYWASRRWREASEQVELYYGERWRDLTPLTQDEKSDIIRAVIGYALSEDVLGLARFREKYAPLMNGGADKLAFDTASKPAASNSAEFEQIARMAAKVNTLSGFLRDMNSRFSGASANAPPPEEAETDPSPTGTLRQIVGSKRVEASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STK4</accession>
    <name>Q3STK4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6468 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1125</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314418.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1125"/>
    <dbReference type="EnsemblBacteria" id="ABA04387">
        <property type="protein sequence ID" value="ABA04387"/>
        <property type="gene ID" value="Nwi_1125"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1125"/>
    <dbReference type="eggNOG" id="ENOG5030V4A">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122383_0_0_5"/>
    <dbReference type="OMA" id="GYCMLLN"/>
    <dbReference type="OrthoDB" id="1588792at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045531">
        <property type="entry name" value="DUF6468"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20072">
        <property type="entry name" value="DUF6468"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="DUF6468" evidence="2">
        <location>
            <begin position="34"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="144" mass="15546" checksum="68FFF34CC2136256" modified="2005-10-11" version="1">MNHSFGLVIESLVAVLLVLTIGYCMLLNKRLKRLRSDEQSLKATISELITATEIAERAIGGLKHTVRDVNDNLGNQIASATDLSRQLAKQLAEGDALMRRLSRIALAARPPTAPEPEAVTSDARSVADAARAFSERRRANGLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STN8</accession>
    <name>Q3STN8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1091</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1091"/>
    <dbReference type="EnsemblBacteria" id="ABA04353">
        <property type="protein sequence ID" value="ABA04353"/>
        <property type="gene ID" value="Nwi_1091"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1091"/>
    <dbReference type="eggNOG" id="COG0392">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056539_0_0_5"/>
    <dbReference type="OMA" id="MIRYRFY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="59"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="95"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="140"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="215"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="246"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="289"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="336"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="376" mass="40683" checksum="DB73DBEA6AB5D37D" modified="2005-10-11" version="1">MRRLLTALQRGFKERIGWKGLGVVASLVIIAFAITTLIHTLQGVDTAVILIALTEKSPYLIAMAALCVVGAFCTLTFYDFFAVRTIGKDHIPYRVAALAAFTSYTIGHNVGATVFTGGAIRFRIYSDYGLNAIDVAKICFISGLTFWLGNLFVLGIGMAWHPWAASAMDLLPPSVNRLIAFGCLAGITIYLAWIITGRNRRELGQNGWKVVLPSAPLTFVQILIGVVDLGFCALAMYLLMPAHPDIDFVSVAVVFILATLLGFASHAPGSLGVFDAAMLVALPQFGKEQMLATLLVFRILYFMIPFALAITIMGTRELWLNVVQPWQRRRKIKNGNSDVSNGNDAGTDPGSSNSESAPDEAASPKPHTARSAVTQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STP4</accession>
    <name>Q3STP4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Dipeptide epimerase</fullName>
            <ecNumber evidence="5">5.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_1085</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4 5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="4 5">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the mandelate racemase/muconate lactonizing enzyme family.</text>
    </comment>
    <dbReference type="EC" id="5.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1085"/>
    <dbReference type="EnsemblBacteria" id="ABA04347">
        <property type="protein sequence ID" value="ABA04347"/>
        <property type="gene ID" value="Nwi_1085"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1085"/>
    <dbReference type="eggNOG" id="COG4948">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030273_4_3_5"/>
    <dbReference type="OMA" id="MFGCYSD"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016855">
        <property type="term" value="F:racemase and epimerase activity, acting on amino acids and derivatives"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03319">
        <property type="entry name" value="L-Ala-DL-Glu_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034603">
        <property type="entry name" value="Dipeptide_epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029065">
        <property type="entry name" value="Enolase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013342">
        <property type="entry name" value="Mandelate_racemase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034593">
        <property type="entry name" value="Mandelate_racemase_DgoD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013341">
        <property type="entry name" value="Mandelate_racemase_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48080">
        <property type="entry name" value="PTHR48080"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13378">
        <property type="entry name" value="MR_MLE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02746">
        <property type="entry name" value="MR_MLE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00010">
        <property type="entry name" value="dipeptide_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00922">
        <property type="entry name" value="MR_MLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="SSF51604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="SSF54826"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MR_MLE" evidence="6">
        <location>
            <begin position="137"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; specific for (R)-substrate epimerization" evidence="3">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; specific for (S)-substrate epimerization" evidence="3">
        <location>
            <position position="252"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR634603-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR634603-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU366006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="333" mass="35623" checksum="AB8D144C391EA752" modified="2005-10-11" version="1">METLSNDVTTLRCRIERWPIAGSFRISRGAKTEAVVVVAEISRSGHTGRGECVPYARYGETPEGVLAAILGMQKPLAAGLDRRSLASAMPAGAARNALDCALCDLEAKCGGQRIWTLLNRPAPSPRSTAYTISLGSPEAMAAATARASRRPLLKIKLGGDGDPQRIAAVRKQARESELIVDANESWTPHNLERNLAACEAAGVTLVEQPLPAGNDEALARIRRPIAVCADESAHDRASLDSLRERYDAINIKLDKTGGLTEALAMADAARALRFEIMIGCMVATSLSMAPAMLIAQHARFVDLDGPLLLARDRDDGLRYDGSTVYPPDAALWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STQ1</accession>
    <name>Q3STQ1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Probable lipid II flippase MurJ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">murJ</name>
        <name evidence="10" type="ordered locus">Nwi_1078</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Involved in peptidoglycan biosynthesis. Transports lipid-linked peptidoglycan precursors from the inner to the outer leaflet of the cytoplasmic membrane.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell inner membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the MurJ/MviN family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314374.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1078"/>
    <dbReference type="EnsemblBacteria" id="ABA04340">
        <property type="protein sequence ID" value="ABA04340"/>
        <property type="gene ID" value="Nwi_1078"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1078"/>
    <dbReference type="eggNOG" id="COG0728">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006797_5_0_5"/>
    <dbReference type="OMA" id="IFFVAFK"/>
    <dbReference type="OrthoDB" id="749401at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015648">
        <property type="term" value="F:lipid-linked peptidoglycan transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13123">
        <property type="entry name" value="MATE_MurJ_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02078">
        <property type="entry name" value="MurJ_MviN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004268">
        <property type="entry name" value="MurJ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03023">
        <property type="entry name" value="MurJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002869">
        <property type="entry name" value="MviN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01806">
        <property type="entry name" value="VIRFACTRMVIN"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01695">
        <property type="entry name" value="murJ_mviN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2 8 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 8 9" id="KW-0133">Cell shape</keyword>
    <keyword evidence="8 9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7 8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 8 9" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="241"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="269"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="306"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="344"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="384"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="412"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="437"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="477"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR002869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA04340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="508" mass="53267" checksum="1628860B9E7AE493" modified="2005-10-11" version="1">MLGRIFTVGGYTLLSRLTGFARDIMLAAILGAGPVADAFFVALRLPNHFRAIFAEGAFNAAFVPAYAHVSGGGPASAKLFASRIFTLLLLSQMVLLAIAWLFMPQVIALLAPGFVDDPARGELAVSLTRITFPYLLLITLVTLYGGMLNVMHRFASAAAAPILLNLSMMMALALAAFFPSAGHAAAWGVLLSGVLQYFLLAADAARHGLMPRLTRPTLDADVRGFFRALGPATVGSMGTQLAMFADTIIATFLPVGALSALYYADRLNQLPVGVIGIAIGTVLLPEMSRRITGGDLAGAMASQRRAFEFSLLFSIPFIAAFLTVPDVIMRAMFARGAFSAADAAAAGATLAAYAVGLIPFVLIRSAVAAFYARKNTATPVKASLAGLGVNLVLKVALMGSLAQVGLALATAAGAWINLLLVLGFAARAGYIEIRRPLLISLGRFLIAGALLAAALWLTAHFASGQFAHWSVMRDEAALGVLIVVGAIVYAGTILLLFGPRWLKSLVRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STS6</accession>
    <name>Q3STS6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Excinuclease ABC, C subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1053</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314349.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1053"/>
    <dbReference type="EnsemblBacteria" id="ABA04315">
        <property type="protein sequence ID" value="ABA04315"/>
        <property type="gene ID" value="Nwi_1053"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1053"/>
    <dbReference type="eggNOG" id="COG2827">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135650_3_1_5"/>
    <dbReference type="OMA" id="WFEAYED"/>
    <dbReference type="OrthoDB" id="2077602at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000305">
        <property type="entry name" value="GIY-YIG_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035901">
        <property type="entry name" value="GIY-YIG_endonuc_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01541">
        <property type="entry name" value="GIY-YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00465">
        <property type="entry name" value="GIYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82771">
        <property type="entry name" value="SSF82771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50164">
        <property type="entry name" value="GIY_YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GIY-YIG" evidence="1">
        <location>
            <begin position="17"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="110" mass="12888" checksum="B20E621ADD9280C3" modified="2005-10-11" version="1">MAGLVPAIHVLQNSCMKGGYVYFLTNRPNGILYVGVTNDLVRRVFEHRSGFVAGFTKRHCLKRLVYFETFDDIRNAIQREHNIKHWPRTWKVRTIVAVNPEWDDLYPTIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU46</accession>
    <name>Q3SU46_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0933</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314236.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0933"/>
    <dbReference type="EnsemblBacteria" id="ABA04195">
        <property type="protein sequence ID" value="ABA04195"/>
        <property type="gene ID" value="Nwi_0933"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0933"/>
    <dbReference type="eggNOG" id="COG1618">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106681_2_0_5"/>
    <dbReference type="OMA" id="FDAWIRF"/>
    <dbReference type="OrthoDB" id="1798498at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018912">
        <property type="entry name" value="DUF2478"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10649">
        <property type="entry name" value="DUF2478"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="187" mass="20368" checksum="A69605E3328342C9" modified="2005-10-11" version="1">MFDAQCDLAALVYTAGEDPDNVLYEFASGLNASGYRAVGIVQLGRHCIDAGLSAMLMPTGEQLQLHQRLDQESAGCRLDVGQLTTAGERIATTLDAGADILIVNRFGRQERKGKGFSRLIERALGDDIPVVIAVPHHHFDDLIKFAGGMTVKLHCDYESLDAWWSLVSTPAGSLRRPDHSTVCEVMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU50</accession>
    <name>Q3SU50_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">SPFH domain, Band 7 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0929</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314232.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0929"/>
    <dbReference type="EnsemblBacteria" id="ABA04191">
        <property type="protein sequence ID" value="ABA04191"/>
        <property type="gene ID" value="Nwi_0929"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0929"/>
    <dbReference type="eggNOG" id="COG0330">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024949_2_2_5"/>
    <dbReference type="OMA" id="TQIRAEM"/>
    <dbReference type="OrthoDB" id="1213601at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032435">
        <property type="entry name" value="Band_7_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_HflKC_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16200">
        <property type="entry name" value="Band_7_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="SSF117892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="PHB" evidence="4">
        <location>
            <begin position="21"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="308"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="332" mass="35992" checksum="3516254EC2EF8A21" modified="2005-10-11" version="1">MTGFDIFAIAVVGLVILTLLAGVKTVPQGHDWTIERFGKYTRTLGPGLNLIIPYIDRVGRKMNMMEQVIEIPQQEVITKDNATVTVDGVAFYQVFDAAKASYEVANLTQSIVTLTMTNIRSVMGSMDLDQVLSHRDEINERLLRVVDAAVTPWGLKVNRIEIKDIVPPADLVQAMGRQMKAEREKRADILQAEGQRQSAILKAEGQKQSQILEAEGRKEAAFRDAEARERSAEAEAEATRMVSEAIAKGDVASLNYFIADKYIKAFGQLANSPNQKVVMLPMEATSMLASLAGIGEIAKATFGDSTAPASAARRGAVPNAGSKVPPPVPPQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU81</accession>
    <name>Q3SU81_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">TonB-dependent receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0896</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0896"/>
    <dbReference type="EnsemblBacteria" id="ABA04160">
        <property type="protein sequence ID" value="ABA04160"/>
        <property type="gene ID" value="Nwi_0896"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0896"/>
    <dbReference type="eggNOG" id="COG4774">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1747712_0_0_5"/>
    <dbReference type="OrthoDB" id="439303at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011662">
        <property type="entry name" value="Secretin/TonB_short_N"/>
    </dbReference>
    <dbReference type="SMART" id="SM00965">
        <property type="entry name" value="STN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="STN" evidence="6">
        <location>
            <begin position="69"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="124"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="149" mass="14914" checksum="EAFB1F75383634A2" modified="2005-10-11" version="1">MGYAARTFSGKAGIGRVLASALMMSTAIIAGVVMTGGDAFAQSAAQTSFNVPAGPLGRVLTAFGRQAGLQVTYLTSIGTGKTSPGISGPATREQALARILQGTGLSYHFTNATTVAISQPAAAGGAGAAPAGRSRSIRSMCRARPRGVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU90</accession>
    <name>Q3SU90_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0887</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314192.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0887"/>
    <dbReference type="EnsemblBacteria" id="ABA04151">
        <property type="protein sequence ID" value="ABA04151"/>
        <property type="gene ID" value="Nwi_0887"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0887"/>
    <dbReference type="eggNOG" id="ENOG5033J7B">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_660277_0_0_5"/>
    <dbReference type="OMA" id="INNTIKW"/>
    <dbReference type="OrthoDB" id="680679at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010567">
        <property type="entry name" value="Clostridium_P47"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06597">
        <property type="entry name" value="Clostridium_P47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="416" mass="44933" checksum="19889AF07D676CBC" modified="2005-10-11" version="1">MVYNTFGWDTVYVIDVNLVNAALRRERLPELEYTSDGTEMTGVMGSMQIVSGGSGKLLRMSFQVAQGSLVSKTVGDASLDGVTLVADLELTLLPSGMPTSQNLVPDIRKVSQQAGPGLLTPVNLLDPGKHLTPAQHALVFALLPQFLVSVAPQITFVLATVNFARPATDSWLAPVKADYAFMNKNDGSNYLGILAVTTDRDVSNLQRTIDDQIVSGSNNAGLFIAESLFMENVIVPVLERAYPDAGRSNFYWNGREVKARRSFGAGSVKKGAITYHPKINSFNLTLVGGTLDTSLSGDCDMKAGIRMSYNASSRNSSRFDMKNKLLTFTSDPHPRFHKDVHIPWYWIVGGAVVEVIVQLVARLIGNAIANALQGGQGVKLARTPPASIQWSHTTNIDVKSAGLNGLFYMQGTAAPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUC1</accession>
    <name>Q3SUC1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Antitoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0856</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Antitoxin component of a type II toxin-antitoxin (TA) system.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the phD/YefM antitoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314165.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0856"/>
    <dbReference type="EnsemblBacteria" id="ABA04120">
        <property type="protein sequence ID" value="ABA04120"/>
        <property type="gene ID" value="Nwi_0856"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0856"/>
    <dbReference type="eggNOG" id="COG2161">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_166832_1_0_5"/>
    <dbReference type="OMA" id="REFNRDV"/>
    <dbReference type="OrthoDB" id="2047723at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006442">
        <property type="entry name" value="Antitoxin_Phd/YefM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036165">
        <property type="entry name" value="YefM-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02604">
        <property type="entry name" value="PhdYeFM_antitox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143120">
        <property type="entry name" value="SSF143120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="84" mass="9221" checksum="2274E70583DE3E43" modified="2005-10-11" version="1">MTITTLSSRELNHDVSSAKKAAQNGPVVITDRGKPSHVLMTYDEFRRLSGKRRSLTQALAMPGLSDIDFDPPRVEIAPRGVDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUG6</accession>
    <name>Q3SUG6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phage terminase GpA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0810</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314123.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0810"/>
    <dbReference type="EnsemblBacteria" id="ABA04075">
        <property type="protein sequence ID" value="ABA04075"/>
        <property type="gene ID" value="Nwi_0810"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0810"/>
    <dbReference type="eggNOG" id="COG5525">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023850_4_1_5"/>
    <dbReference type="OMA" id="RAEPYPL"/>
    <dbReference type="OrthoDB" id="576433at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_04144">
        <property type="entry name" value="TERL_LAMBDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008866">
        <property type="entry name" value="Phage_lambda_GpA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05876">
        <property type="entry name" value="Terminase_GpA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="614" mass="68767" checksum="47841C0ABEF664DF" modified="2005-10-11" version="1">MSDAISKNSAKSASSFDEGFDGRAEIITAWSRGLAPDPALTVSAWADRYRFLSSRASSEAGRYRTDRTPYMRGVMDALSPGSSARRIVFMKAAQVGATEAGNNWIGYCIHQAPGPFLGVQPTTDLAKRLSQQRIEPLIDESPELRALILPSRSRDSGNTVLAKKFAGGQLVLTGANSAVGLRSMPARYVFLDEVDAYEGDVDGEGDPVALAIARTRTFGHRAKVFLVSTPTIKGLSRIEREFEASDQRRFFVPCPHCGLSQWLKFERLKWTSGEPASAAYHCEACDQPIAEHHKTAMLSAGEWRATATPADPHCVGFHISGLYSPVGWLGWADIAREWEAAQGDDAALKAAKNTLLGETWQERGEAPDWQRLYERREDFAPLVPMCGLILTAGADVQHDRIEVDIWAWGRRLTSALVEHIVLEGDTSREEVWGKLTALLGQTWRHENGARMRIARLAIGRTIRRGVKLWKVSVAVFKSETYRFLRLDRPTDEELAADTPFPDGFMHLPKSVTAEWVKQLVAEQLVTVRDRRGFSKLEWRQMRERNEALDCRVYARAAAWLLGIDRFDDAKFEALEEELRVAAEDEARPVDQRGVTPTTAPVRRSEWLGRRDKWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUH0</accession>
    <name>Q3SUH0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0806</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314119.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0806"/>
    <dbReference type="EnsemblBacteria" id="ABA04071">
        <property type="protein sequence ID" value="ABA04071"/>
        <property type="gene ID" value="Nwi_0806"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0806"/>
    <dbReference type="eggNOG" id="COG5471">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165535_1_0_5"/>
    <dbReference type="OMA" id="FWDATAK"/>
    <dbReference type="OrthoDB" id="1773677at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011231">
        <property type="entry name" value="Phage_VT1-Sakai_H0018"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09956">
        <property type="entry name" value="DUF2190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF030771">
        <property type="entry name" value="UCP030771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="116" mass="11661" checksum="580B48632BAC4D9C" modified="2005-10-11" version="1">MKNFDQAGDVVTVAAPYALTSGQGAKTGQLFGVAIGDAANGADVALKTSGVFDLTKIGSQAWTAGQLVYWDDTNKRCTSVATGSLLIGVAVAAVAGGAGNTVGRVRLNASFRANEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUH5</accession>
    <name>Q3SUH5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0801</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314114.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0801"/>
    <dbReference type="EnsemblBacteria" id="ABA04066">
        <property type="protein sequence ID" value="ABA04066"/>
        <property type="gene ID" value="Nwi_0801"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0801"/>
    <dbReference type="eggNOG" id="ENOG502ZBQM">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149361_0_0_5"/>
    <dbReference type="OMA" id="SPLRYHY"/>
    <dbReference type="OrthoDB" id="1700288at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="144" mass="15034" checksum="6187578FCD4DDECE" modified="2005-10-11" version="1">MASKRETVLAAVKALVAAALPGAEVKRNLAKAERIPPGGLVVIRDGDPGEPEISLSPLTYLYSHRIPIEIATYESATLTREQVLDAMTGAIGAAVKANPTLGGLCDWIEAEAPVTDDIEALGALPDRFADLAILAAYATSDPLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUK3</accession>
    <name>Q3SUK3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cytochrome c, class I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_0773</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314087.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0773"/>
    <dbReference type="EnsemblBacteria" id="ABA04038">
        <property type="protein sequence ID" value="ABA04038"/>
        <property type="gene ID" value="Nwi_0773"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0773"/>
    <dbReference type="eggNOG" id="COG2010">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073827_0_0_5"/>
    <dbReference type="OMA" id="ERRDEAW"/>
    <dbReference type="OrthoDB" id="1469969at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00034">
        <property type="entry name" value="Cytochrom_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229137" evidence="7">
        <location>
            <begin position="33"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="8">
        <location>
            <begin position="33"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="8">
        <location>
            <begin position="140"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="305"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA04038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="326" mass="34094" checksum="CF9552EA763304E0" modified="2005-10-11" version="1">MSKKPLASWRFMPVVALATTAIWAALPTMANAADAAAGKKLFDDKTCSACHSIGGGDLVGPDLKNVTKERPREWLHEWIMKPDAMIAKKDPVAVELLGKYNGMEMPNMGLTSAEVDNIIAFLEGGGAGASSAAAAPALKGDAENGKNLFTGNEKFANGGPPCMACHSSGGLGAFGGGALGPDLTTAAKRLGGASGLSSWLAGLPTPTMKAVWTKQPPTPQERADVAAFLSQEGLAVRSASAIWQLAGLTGLGAVVLLLIAGFRWRNRLKFGVRRPMMATPTTGRSTGPYNGGWFTGTYSDGWIGRFKGTDGNSNRPRGRTNAPRRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUU4</accession>
    <name>Q3SUU4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Sulfonate/nitrate transport system substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0681</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0681"/>
    <dbReference type="EnsemblBacteria" id="ABA03947">
        <property type="protein sequence ID" value="ABA03947"/>
        <property type="gene ID" value="Nwi_0681"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0681"/>
    <dbReference type="eggNOG" id="COG0715">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170836_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="84" mass="8875" checksum="57DBAA702441A49F" modified="2005-10-11" version="1">MIDALADIDRWADGKEAAVAEELSAGIGIPAPVLEIALKRQTYGIRPLDDKVVASQQSIADTFHALGLLPKPLVVSSIVRKAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUX2</accession>
    <name>Q3SUX2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Beta-lactamase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0653</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313979.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0653"/>
    <dbReference type="EnsemblBacteria" id="ABA03919">
        <property type="protein sequence ID" value="ABA03919"/>
        <property type="gene ID" value="Nwi_0653"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0653"/>
    <dbReference type="eggNOG" id="COG1680">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020027_14_3_5"/>
    <dbReference type="OMA" id="CEKDYFA"/>
    <dbReference type="OrthoDB" id="537440at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021860">
        <property type="entry name" value="Peptidase_S12_Pab87-rel_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11954">
        <property type="entry name" value="DUF3471"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229152" evidence="2">
        <location>
            <begin position="30"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-lactamase" evidence="3">
        <location>
            <begin position="60"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3471" evidence="4">
        <location>
            <begin position="430"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="257"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF11954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="531" mass="56122" checksum="88CAF161C6F3FCC7" modified="2005-10-11" version="1">MFLQKSVVAFRPRAILAKLVASSLVWACAAIPGFAETSPPQTSGVPIADGQIDEAVAGLDAIATGVMKKTGIPGMAVAVVRDGKIAYAKGFGVRKVGENQPIDANTVFQIASLSKSIAATVVARQVGADVVKWNTPVQAHLPWFRLMDPWVTQHVTIADLFSHRSGLPDHAGDDLEDLGYGRRAVLERLRLLPLHGFRDVYAYTNFGLTAAAEAVAAASGKDWESLSEEVLYKPLGMSATSSRFADFEKQANRAIGHVRGGDGFQPKYQRRPDAQSPAGGVSSSANDLARWLALVLRNGEFEGRQVIPADALLPAMTAEVVSIHSPSADARASLYGYGFGVGVTPAGRTMISHSGAFALGAGTHYTIIPSAGIGIVVLTNAEPVGAAEAVGASFMDLVQFGKITRDWLAAYSGVMAQLTAPAGDLVGKSPPAKPAPAASSTSYVGRYGSPYFGDAEIVDANGHMELKIGRLSRSYTLTHWDGDVFSIKPSSENQTDGSVSSVTFKRTGDEPASELTIEFLNDNGLGQFMRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUY3</accession>
    <name>Q3SUY3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0641</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0641"/>
    <dbReference type="EnsemblBacteria" id="ABA03908">
        <property type="protein sequence ID" value="ABA03908"/>
        <property type="gene ID" value="Nwi_0641"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0641"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_514657_0_0_5"/>
    <dbReference type="OMA" id="VTSHVDY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="535"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="559" mass="62755" checksum="CD27C85E8E3F64F5" modified="2005-10-11" version="1">MSISEFLEENRRFWTSSTPPRSERVIVLDLYHNANVHSHLFANCVVAKCIQHIWGGEIVGIFTDDFGDDRKTDVEAKQAIARSFGVSRFYNISEFRNRLLSDPVWSANVESDLLGRLEDKSGPSLRNAVRLLSEPKFPRRGLLIYNEFLRRFGSSTIDDLSDELIAIAKLVSADYEAIAMVLGQSSVRASVLGHIGYSVSGQIADFSAMRGAPTFLVEQYIPIAVTRYRDLTDLSSGLPGDLKEMLESYAFDQALLGDPRLDLFDGAVEATLARTIDPLLNRDAVDQPPVSREDFLENCGVDPKNPTVCVFAHVFQDAPLTRPKQLSDDYWQAIVDVVDLARQLTHLNLIIKPHPLNAIYDRERSWDRLREYCAEIKNVGFADGDLSAGEVMKHCDAGLTVRGTVLYEMAAAGHRMIATGEDKFSGLGIIEEASSMEALKSILMSVSREAWSMPAEQRQRALAYSYCLLQGFRSKSILVPDKRWPPDEYFRSATLALSNFRLEEDPLFINLKWFLRNDLPMLISKEAFPEMSSKRFATKAQSLQGSDQQTSDRDPGCSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUY7</accession>
    <name>Q3SUY7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Acyl-CoA dehydrogenase</fullName>
            <ecNumber evidence="10">1.3.8.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_0637</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.1" evidence="10"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313964.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0637"/>
    <dbReference type="EnsemblBacteria" id="ABA03904">
        <property type="protein sequence ID" value="ABA03904"/>
        <property type="gene ID" value="Nwi_0637"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0637"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_12_2_5"/>
    <dbReference type="OMA" id="IEFARMM"/>
    <dbReference type="OrthoDB" id="433393at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004085">
        <property type="term" value="F:butyryl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025878">
        <property type="entry name" value="Acyl-CoA_dh-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12806">
        <property type="entry name" value="Acyl-CoA_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0274">FAD</keyword>
    <keyword evidence="3 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="8">
        <location>
            <begin position="40"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="7">
        <location>
            <begin position="160"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="6">
        <location>
            <begin position="282"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_C" evidence="9">
        <location>
            <begin position="464"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA03904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="592" mass="62351" checksum="C1BE8A930D2703CC" modified="2005-10-11" version="1">MTYRAPISDMLLALDGAGLRAAVEAGHYGDFDAEITTVVLEEAGRFASDVLAPLNRAGDRHGARLQDGRVTTAPGWADAYRRWTAAGWNAVSGPEDFGGQGLPLAINAACTEIWSAANTAFGLCPLVTLSAVEALAAHGSDTLKTIYLGKLVSGEWTGTMQLTEPQAGSDVGALRSRAERASDGSYRIFGTKIFITYGDHDMTDNIVHFVLARLPDAPDGTRGISLFLIPKFLVDADGSLGARNDIHASGIEHKLGIHGSPTCTMTMGDDGGAIGYLIGEENRGMRCMFTMMNQARLCVGLEGVGIAERASQQALAYARERRQGRAAGTSDGMSPIIAHPDVKRMLMQMRALTAAARAICYATAVSLDMAARAADPDVRTAAAARGALLTPIAKAFSTDIGNEVASLGIQVHGGMGFIEETGAAQHYRDARITSIYEGTNGIQAIDLVTRKLAADDGAAVSALLDELSDTVKRVEASNDPAFGTTGERLRDALGSLERAGKWLLERLESSPNDALAGATPYLRLFGSTLGGCKLAEEALAARESSAEFGDPRRYVTLARFFAETIAVQAPALERTVIDGADAVNGADTVLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV08</accession>
    <name>Q3SV08_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Pirin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0616</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the pirin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313943.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0616"/>
    <dbReference type="EnsemblBacteria" id="ABA03883">
        <property type="protein sequence ID" value="ABA03883"/>
        <property type="gene ID" value="Nwi_0616"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0616"/>
    <dbReference type="eggNOG" id="COG1741">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045717_5_1_5"/>
    <dbReference type="OMA" id="TPWHPHR"/>
    <dbReference type="OrthoDB" id="833478at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012093">
        <property type="entry name" value="Pirin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008778">
        <property type="entry name" value="Pirin_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003829">
        <property type="entry name" value="Pirin_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02678">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05726">
        <property type="entry name" value="Pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006232">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pirin" evidence="3">
        <location>
            <begin position="21"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Pirin_C" evidence="4">
        <location>
            <begin position="183"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="293" mass="31256" checksum="64D281EE6F70AE41" modified="2005-10-11" version="1">MRKVLGVFSGPRQHWVGDGFPVRSLFSHASHGDHVSPFLLLDYAGPADFTPAERPRGVGVHPHRGFETVTIVYQGEVEHRDSTGNGGLIGPGDVQWMTAASGILHEEFHSRAFTKKGGALEMVQLWVNLPARDKNAEPGYQTLLNADIPSVDLPDGAGRLRVIAGEFDGRKGPAKTFTPVGIYDVKLNRDGVATLNPPEGHTVAVVVLKGTVLVGGSEIAREAQFALLDRNGGEVTIEANGDALVLILSGVPIDEPVVMRGPFVMNTEGEIRQAMIDFQSGRFGTLEPVGAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV12</accession>
    <name>Q3SV12_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0612</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03879">
        <property type="protein sequence ID" value="ABA03879"/>
        <property type="gene ID" value="Nwi_0612"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0612"/>
    <dbReference type="HOGENOM" id="CLU_2524185_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="84" mass="9086" checksum="B6EC1DC6599C0223" modified="2005-10-11" version="1">MNVIDSNILERDAGGKPLHTFPHPALGAGIHRTQSGVAKYPLVRPDRGISRSKDSASSDDCRTPRSRRRTRLGLTGQPGSKPGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV86</accession>
    <name>Q3SV86_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lipopolysaccharide biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0538</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313866.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0538"/>
    <dbReference type="EnsemblBacteria" id="ABA03805">
        <property type="protein sequence ID" value="ABA03805"/>
        <property type="gene ID" value="Nwi_0538"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0538"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_377583_0_0_5"/>
    <dbReference type="OMA" id="ILICNAM"/>
    <dbReference type="OrthoDB" id="150988at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd11579">
        <property type="entry name" value="Glyco_tran_WbsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032719">
        <property type="entry name" value="WbsX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41244">
        <property type="entry name" value="PTHR41244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14307">
        <property type="entry name" value="Glyco_tran_WbsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="734" mass="83393" checksum="9D191DEEB59E7F40" modified="2005-10-11" version="1">MNKIAERDVRSAKGTRPVASPISHEDNLQSSFLDPDLDDLESGARLRGAILEQSGREIFGWIVDARSLATSVSLSLFVGSHAIEETRTERAAPAGVGSGMAIGRPGFRIDLANPVARRRLARALGNTLADPGQTQVYLRVRLDDGEFFEIPLFDALAPEELVVALGFSPSDYTDLLIPNNNRRITPDPDPAGKQVRHVCFYLPQFHSFEENDRWWGPGFTEWTNISRMRPQFPAHESPWLPADLGFYDVRLTETRRQQAELARRYGIHGFCYYVYWFQGRRLLERPLELLLEDGEPDLPFCICWANENWSRRWDGSDAELLLGQNHALEDDVRFIDDMAQLLLDERYIKVDGRKIILVYRPSLLPDRQSLFDTWRARAKQLGIGDILICNAMTFGEFDPRDLSCDAAVEFPPHTVSTREQPPSEFGAPESFTGKVYDYVDAVRSSLGKTYDFPYFPAVMPRWDNTPRKGSRGHVFNRSSPEAFEVWLRDATGRARRGPFAEPIVFINSWNEWAEGAHLEPDSRYGRAFLEAVRRVASSEPISAQVRSNPDLLDCLSRQELEQAMKEASTHVQLLANIHRSEMVYSGLQHMRAGMPVEAEGAPENPAESLLVIENINSQTNERVVVDPRSAVLVRGWVCAGDTFEASKQRIAFLILLSTDSRKQDHNGYCLVVDWHERFDVKEYMRTRSSDCYFGFQLSFLVHDLPEGEYQIAVLEVGEGNRALIRSNHSIIIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV96</accession>
    <name>Q3SV96_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0528</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313856.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03795">
        <property type="protein sequence ID" value="ABA03795"/>
        <property type="gene ID" value="Nwi_0528"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0528"/>
    <dbReference type="eggNOG" id="ENOG5032HXN">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_193825_0_0_5"/>
    <dbReference type="OMA" id="ECARHIA"/>
    <dbReference type="OrthoDB" id="2065063at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="66" mass="6934" checksum="A7B7C84C6E5C4A3A" modified="2005-10-11" version="1">MNHSIYSADRTTHLKIVVVALVAGIAVAGFAISARMNADDGYTQTARIIKAGKPMAVTSSDAMVVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVC5</accession>
    <name>Q3SVC5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0499</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03766">
        <property type="protein sequence ID" value="ABA03766"/>
        <property type="gene ID" value="Nwi_0499"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0499"/>
    <dbReference type="HOGENOM" id="CLU_2001457_0_0_5"/>
    <dbReference type="OrthoDB" id="1925751at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="124" mass="13593" checksum="24D0DD725681F790" modified="2005-10-11" version="1">MLAGAQCSGTTHSPQGGTDDDNHGNRPDIWDRRQCRAAGPRQRDKIHGIPANISRRAGEASACSAFRARHDVFFIAQLIAIAEHCPQTRIFRRASPQVAQAAYHSTGERKQVGARTGPRLLRVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVJ2</accession>
    <name>Q3SVJ2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">OsmC-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0432</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the OsmC/Ohr family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313763.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0432"/>
    <dbReference type="EnsemblBacteria" id="ABA03699">
        <property type="protein sequence ID" value="ABA03699"/>
        <property type="gene ID" value="Nwi_0432"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0432"/>
    <dbReference type="eggNOG" id="COG1764">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106355_2_1_5"/>
    <dbReference type="OMA" id="TPEKGFK"/>
    <dbReference type="OrthoDB" id="1984062at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019953">
        <property type="entry name" value="OHR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003718">
        <property type="entry name" value="OsmC/Ohr_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036102">
        <property type="entry name" value="OsmC/Ohrsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33797">
        <property type="entry name" value="PTHR33797"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02566">
        <property type="entry name" value="OsmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82784">
        <property type="entry name" value="SSF82784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03561">
        <property type="entry name" value="organ_hyd_perox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="134" mass="14127" checksum="76B363BAC5693365" modified="2005-10-11" version="1">MAYVTKATTKGGRNGRAILDDGGLGLAMALPKEMGGSGEGHNPEQLFALGWSSCFGQAVLALARKHGLDGQAAEIGCYVSIDKDEISFALKAELTLRIPGADKEKVKALLEDAHTICPYSRATRNNVPVTLSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVK8</accession>
    <name>Q3SVK8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Lysophospholipase L1-like esterase</fullName>
            <ecNumber evidence="3">3.1.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0416</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.1.2" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0416"/>
    <dbReference type="EnsemblBacteria" id="ABA03683">
        <property type="protein sequence ID" value="ABA03683"/>
        <property type="gene ID" value="Nwi_0416"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0416"/>
    <dbReference type="eggNOG" id="COG2755">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051180_1_1_5"/>
    <dbReference type="OMA" id="MRIPPNY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004064">
        <property type="term" value="F:arylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008265">
        <property type="entry name" value="Lipase_GDSL_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01098">
        <property type="entry name" value="LIPASE_GDSL_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SGNH_hydro" evidence="2">
        <location>
            <begin position="69"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="251" mass="26364" checksum="FB17AEF58724A0C8" modified="2005-10-11" version="1">MWSRLLPYGRPPEHLKHNDGDRTGRRAGSYGISFSTIERLTRGFVLLLLGAVLVLGAPARAADPVRIVVLGDSLSAGLGLSAADAFPAQLEKALKDKGVNVAISNAGVSGDTASGGLDRLDWSVPAGTQGVILELGANDALRGIDPKVTRAALDEILKRLKARNIAILLCGMVAPPNYGDEYAAQFGAIFSDLAKKHGVPLYPFFLEGVAANAELNQADGIHPTAAGVTTIVKNILSTVEAFLDTLSERKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVS3</accession>
    <name>Q3SVS3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Beta-lactamase</fullName>
            <ecNumber evidence="2 4">3.5.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_0351</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a beta-lactam + H2O = a substituted beta-amino acid</text>
            <dbReference type="Rhea" id="RHEA:20401"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35627"/>
            <dbReference type="ChEBI" id="CHEBI:140347"/>
            <dbReference type="EC" id="3.5.2.6"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the class-A beta-lactamase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.6" evidence="2 4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313683.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0351"/>
    <dbReference type="EnsemblBacteria" id="ABA03618">
        <property type="protein sequence ID" value="ABA03618"/>
        <property type="gene ID" value="Nwi_0351"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0351"/>
    <dbReference type="eggNOG" id="COG2367">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031960_6_0_5"/>
    <dbReference type="OMA" id="FKTLACA"/>
    <dbReference type="OrthoDB" id="991474at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008800">
        <property type="term" value="F:beta-lactamase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030655">
        <property type="term" value="P:beta-lactam antibiotic catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045155">
        <property type="entry name" value="Beta-lactam_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000871">
        <property type="entry name" value="Beta-lactam_class-A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023650">
        <property type="entry name" value="Beta-lactam_class-A_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35333">
        <property type="entry name" value="PTHR35333"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13354">
        <property type="entry name" value="Beta-lactamase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00118">
        <property type="entry name" value="BLACTAMASEA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00146">
        <property type="entry name" value="BETA_LACTAMASE_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0046">Antibiotic resistance</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase2" evidence="5">
        <location>
            <begin position="59"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA03618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="296" mass="30940" checksum="96D950B241D5FC08" modified="2005-10-11" version="1">MISRRGLWPVGAGLAGLVVMGRAGFAVAPAALQDRFVEELKRLENQTGGRLGVALLDAGTGQHVGHRMDEPFPMCSTFKVLAAGALLQGVDAGKEDLARRIYFNEADLVAHSPETRKHVGPTGMTVAELCKAAITLSDNTAGNLLLASIGGPQGLTAFARRLGDEVTRLDRIETELNESVPGDPRDTTTPSAMAANLRALALGDVLSVKSRTQLVGWLVANTTGDKRLCAGLPAGWRAGDKTGTGERGTANDVAVIWPPDRAPFVVTAYLTGATVPADRQNAVMAAIGRAVTTVLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVU7</accession>
    <name>Q3SVU7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="13">ABC transporter, transmembrane region</fullName>
            <ecNumber evidence="13">3.6.3.27</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Nwi_0327</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in beta-(1--&gt;2)glucan export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.27" evidence="13"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313659.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0327"/>
    <dbReference type="EnsemblBacteria" id="ABA03594">
        <property type="protein sequence ID" value="ABA03594"/>
        <property type="gene ID" value="Nwi_0327"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0327"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_3_5"/>
    <dbReference type="OMA" id="HTFVAVT"/>
    <dbReference type="OrthoDB" id="643917at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="3 10 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="12">
        <location>
            <begin position="27"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="344"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="377"/>
            <end position="384"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA03594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="587" mass="64002" checksum="B1FE0CDB8E8BFA58" modified="2005-10-11" version="1">MDDLSEYASRPFAFVMRYCRRRSASHIAILSCVVAAVVCSVGTQYGVKSLVDALTRGPGHSGAVWLAFIFLVTLIAADNLLWRVASWVASFTFVGVTGDLRRDIFRHLTGHAPSYFARRMPGMLTSRITATSNAVHTVENMLVWNVLPPCIATVVAIALIGTVSLPMAAGLALVAVFMVLVMFGVAAGGKTLHDDYADKAAMVDGEMVDVVNNMPLVRAFCGTDHELDRFNATVGREMSARGRSLRYLEKLRIAHAVITIVLTISLLAWAITLWQRGSVTTGDVVLVCTLGLSILHATRDLAVALVDVTQHVARLSEAVATLLVPHELTDHPEARPLRKNGAAIAFNNVSFRYPGGRSVFERFNLRIQPGQRVGLVGESGGGKSTLFVLLQRFYDAERGSVTIDNQDISRVTQHSLREAISVVPQDISLFHRTILENIRYGRPTATDDEVLRAASAARCDFVDNLPEGFATMVGNRGVKLSGGQRQRIAIARAFLKDTPILLLDEATAALDSESEEAVREALGSLMRGRTVIAIAHRLSTLRSFDRVVVLKNGRIIEDGQPACLMRKDGHYRNLVAKEMSRLAVHAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVV8</accession>
    <name>Q3SVV8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Signal recognition particle receptor FtsY</fullName>
            <shortName evidence="5">SRP receptor</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ftsY</name>
        <name evidence="7" type="ordered locus">Nwi_0316</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC). Interaction with SRP-RNC leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. SRP is a ribonucleoprotein composed of Ffh and a 4.5S RNA molecule.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell inner membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the GTP-binding SRP family. FtsY subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313648.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0316"/>
    <dbReference type="EnsemblBacteria" id="ABA03583">
        <property type="protein sequence ID" value="ABA03583"/>
        <property type="gene ID" value="Nwi_0316"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0316"/>
    <dbReference type="eggNOG" id="COG0552">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009301_3_2_5"/>
    <dbReference type="OMA" id="GISDQFQ"/>
    <dbReference type="OrthoDB" id="656414at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031226">
        <property type="term" value="C:intrinsic component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00920">
        <property type="entry name" value="FtsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004390">
        <property type="entry name" value="SR_rcpt_FtsY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134:SF7">
        <property type="entry name" value="PTHR43134:SF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="SSF47364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00064">
        <property type="entry name" value="ftsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 5" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRP54" evidence="6">
        <location>
            <begin position="285"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="118"/>
            <end position="125"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="200"/>
            <end position="204"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="264"/>
            <end position="267"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="315" mass="33279" checksum="22E23D1F9E351C8F" modified="2005-10-11" version="1">MSDSSEDQPKQSWWRRLSGGLKRTSASLGTAVADLVTKRKLDRAMLDDIEDVLLRADLGTAVAERIAEAVGEGRYDKAISADEVKAVVATEVEKVLAPVARPLIIDETVKPFVILVVGVNGSGKTTTIGKLAAKLSAEGRKVMMAAGDTFRAAAIDQLKVWGERTGSPVIAGAQGSDSASLAFNALTEARNQNSDVLLIDTAGRLQNKAELMNELEKMVRVIRRVDSSAPHVVLLVLDATVGQNALSQVEAFHRTAGVTGLVMTKLDGTARGGILVALAEKHKLPVHFIGVGEGVDDLAPFTAGDFARAVAGIEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW34</accession>
    <name>Q3SW34_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FoF1 ATP synthase, subunit I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0239</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313573.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0239"/>
    <dbReference type="EnsemblBacteria" id="ABA03507">
        <property type="protein sequence ID" value="ABA03507"/>
        <property type="gene ID" value="Nwi_0239"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0239"/>
    <dbReference type="eggNOG" id="COG5336">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137927_0_0_5"/>
    <dbReference type="OMA" id="FVGTAPW"/>
    <dbReference type="OrthoDB" id="2073672at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032820">
        <property type="entry name" value="ATPase_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09527">
        <property type="entry name" value="ATPase_gene1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="127" mass="13236" checksum="090CBDDED6B43A08" modified="2005-10-11" version="1">MSDGTDKNSGGHGDREHSAPDEAALSERLGNLDQRLSRLRGPGRSETDRSGDGSGDGAARASAMARGFRLSSELIAGVAVGALIGWGVDRLLSTSPWGLIVFFLLGFAAGVINVMRSAGVIPEQKDR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW38</accession>
    <name>ATPF1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP synthase subunit b 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP synthase F(0) sector subunit b 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">ATPase subunit I 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">F-type ATPase subunit b 1</fullName>
            <shortName evidence="1">F-ATPase subunit b 1</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpF1</name>
        <name type="ordered locus">Nwi_0235</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Component of the F(0) channel, it forms part of the peripheral stalk, linking F(1) to F(0).</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">F-type ATPases have 2 components, F(1) - the catalytic core - and F(0) - the membrane proton channel. F(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). F(0) has three main subunits: a(1), b(2) and c(10-14). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. F(1) is attached to F(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase B chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313570.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SW38"/>
    <dbReference type="SMR" id="Q3SW38"/>
    <dbReference type="STRING" id="323098.Nwi_0235"/>
    <dbReference type="EnsemblBacteria" id="ABA03503">
        <property type="protein sequence ID" value="ABA03503"/>
        <property type="gene ID" value="Nwi_0235"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0235"/>
    <dbReference type="eggNOG" id="COG0711">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079215_6_1_5"/>
    <dbReference type="OMA" id="KVPGMMA"/>
    <dbReference type="OrthoDB" id="1585587at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045263">
        <property type="term" value="C:proton-transporting ATP synthase complex, coupling factor F(o)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01398">
        <property type="entry name" value="ATP_synth_b_bprime"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002146">
        <property type="entry name" value="ATP_synth_b/b'su_bac/chlpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00430">
        <property type="entry name" value="ATP-synt_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0138">CF(0)</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000368627" description="ATP synthase subunit b 1">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01398"/>
        </source>
    </evidence>
    <sequence length="161" mass="17217" checksum="40DD12C7BDE04905" modified="2005-10-11" version="1">MLAEPETWVAIAFLLLMGVFAYVGVHRTVLSALDRRSARIKNELDDARRLKDEAAKLLADYKARHASAEREAQDIIASARAEAERIAAEAKAKMEDFVARRTKSAEGKIASAEAQAIADVRAAAADAAVAAASSILSNSVKGQLADELLVQGVSEVRSKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW53</accession>
    <name>Q3SW53_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0220"/>
    <dbReference type="EnsemblBacteria" id="ABA03488">
        <property type="protein sequence ID" value="ABA03488"/>
        <property type="gene ID" value="Nwi_0220"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0220"/>
    <dbReference type="HOGENOM" id="CLU_1523613_0_0_5"/>
    <dbReference type="OMA" id="YEARAEM"/>
    <dbReference type="OrthoDB" id="2138621at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="176" mass="20106" checksum="DEADE9746E64B5A1" modified="2005-10-11" version="1">MPVLRYFVVMGCCLMGLLYAVDLMWPGEREAKVDYYSTPVVVAKPSSAESGFASRIERAVGETETPGSMENRRYPGQRFERTKQDSRPVVYPNIATLAPTPHRLQWERQLRYLPANHVYEARAEMPKEAIPEQPKPTARAATRKQLAHARPARIAPDFERPRYARNAGWDPFGLFD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW79</accession>
    <name>HRCA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Heat-inducible transcription repressor HrcA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hrcA</name>
        <name type="ordered locus">Nwi_0194</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Negative regulator of class I heat shock genes (grpE-dnaK-dnaJ and groELS operons). Prevents heat-shock induction of these operons.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HrcA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313530.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SW79"/>
    <dbReference type="SMR" id="Q3SW79"/>
    <dbReference type="STRING" id="323098.Nwi_0194"/>
    <dbReference type="EnsemblBacteria" id="ABA03462">
        <property type="protein sequence ID" value="ABA03462"/>
        <property type="gene ID" value="Nwi_0194"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0194"/>
    <dbReference type="eggNOG" id="COG1420">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050019_0_0_5"/>
    <dbReference type="OMA" id="DYPGTMG"/>
    <dbReference type="OrthoDB" id="1510750at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00081">
        <property type="entry name" value="HrcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002571">
        <property type="entry name" value="HrcA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021153">
        <property type="entry name" value="HrcA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023120">
        <property type="entry name" value="WHTH_transcript_rep_HrcA_IDD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34824">
        <property type="entry name" value="PTHR34824"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01628">
        <property type="entry name" value="HrcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005485">
        <property type="entry name" value="HrcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00331">
        <property type="entry name" value="hrcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0678">Repressor</keyword>
    <keyword id="KW-0346">Stress response</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_1000010437" description="Heat-inducible transcription repressor HrcA">
        <location>
            <begin position="1"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00081"/>
        </source>
    </evidence>
    <sequence length="362" mass="39127" checksum="08C5070B23D8C9BE" modified="2005-10-11" version="1">MAHHDPIGLIAPNAGLAQLNERSRDIFRQIVESYLATGEPVGSRNISRLIAVPLSPASVRNVMSDLEQLGLIYAPHTSAGRLPTELGLRFFVDALMQVGDLTEPERQSIQSQLASVGKAQSVEAALEEALTRLSGLTRTAAVVLTAKSNVRLKHIEFVRLEPDKALVVLVAEDGQVENRVLTLPSGVPASALTEASNFLNARIRGRTLAEARLELETALAQSKAELDQLTQKIVAAGIASWSGGDSDDRQLIVRGHANLLEDLHAMEDLERVRLLFDDLETKREVIDLLGRAERADGVRIFIGSENKLFSLSGSSTIIAPYSDGAGHIVGVLGVIGPTRLNYARVIPMVDYAARIVSQMLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWG2</accession>
    <name>Q3SWG2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Helix-turn-helix, Fis-type</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0111</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313448.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0111"/>
    <dbReference type="EnsemblBacteria" id="ABA03379">
        <property type="protein sequence ID" value="ABA03379"/>
        <property type="gene ID" value="Nwi_0111"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0111"/>
    <dbReference type="eggNOG" id="COG4567">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_6_5"/>
    <dbReference type="OMA" id="RRLEWEH"/>
    <dbReference type="OrthoDB" id="930905at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="14"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="184" mass="20045" checksum="CD8B5FBA03B4FEA4" modified="2005-10-11" version="1">MNATPDVSPQADRSLLIVEDDKPFLERLSRAMETRGFNVTPCDSVSDGLAHIGKSAPAFAVVDLRLGDGNGLDVVSALKRERPDARAIVLTGYGNIATAVTAVKLGAIDYLSKPADADDVVAALLASGTAKSEVPPNPMSADRVRWEHIQRIYEMCNRNVSETARRLNMHRRTLQRILAKRAPR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWL7</accession>
    <name>TRPF_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-(5'-phosphoribosyl)anthranilate isomerase</fullName>
            <shortName evidence="1">PRAI</shortName>
            <ecNumber evidence="1">5.3.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trpF</name>
        <name type="ordered locus">Nwi_0056</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:21540"/>
            <dbReference type="ChEBI" id="CHEBI:18277"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="EC" id="5.3.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TrpF family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.24" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313395.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWL7"/>
    <dbReference type="SMR" id="Q3SWL7"/>
    <dbReference type="STRING" id="323098.Nwi_0056"/>
    <dbReference type="EnsemblBacteria" id="ABA03324">
        <property type="protein sequence ID" value="ABA03324"/>
        <property type="gene ID" value="Nwi_0056"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0056"/>
    <dbReference type="eggNOG" id="COG0135">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076364_1_1_5"/>
    <dbReference type="OMA" id="FYAKSPR"/>
    <dbReference type="OrthoDB" id="1854712at2"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00042"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004640">
        <property type="term" value="F:phosphoribosylanthranilate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00405">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00135">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001240">
        <property type="entry name" value="PRAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044643">
        <property type="entry name" value="TrpF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42894">
        <property type="entry name" value="PTHR42894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00697">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="chain" id="PRO_1000018616" description="N-(5'-phosphoribosyl)anthranilate isomerase">
        <location>
            <begin position="1"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00135"/>
        </source>
    </evidence>
    <sequence length="225" mass="23863" checksum="9CFAD7A29019E07A" modified="2005-10-11" version="1">MSLIVKICGLSTPSTLDVALQAGADMVGFVFFPPSPRHLELARAQELGAQVRGRAAKVALTADADDETLCGIIEALRPDLLQLHGKETVPRIREIKRRFGLPVMKAIGVEIAADLADLPRYAAVADRLLFDARPPKHATRPGGLGVPFDWRLLTNLSVDIPFMLSGGLAAGNVDDAVRITRAGGVDVSSGVESAPGVKDAGMVRDFIRAARAAETSLREATSHVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="126" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWM8</accession>
    <name>Q3SWM8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0045</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313384.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0045"/>
    <dbReference type="EnsemblBacteria" id="ABA03313">
        <property type="protein sequence ID" value="ABA03313"/>
        <property type="gene ID" value="Nwi_0045"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0045"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018130_0_0_5"/>
    <dbReference type="OMA" id="NEFVQHV"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="6">
        <location>
            <begin position="613"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="84"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="838" mass="91905" checksum="BD5A3E1B57A3D11E" modified="2005-10-11" version="1">MSGVIGVMRRTLLSCTSLVPGGIAGAGAVSLIDTSPAWAAEPALNERLIQAFMNLSRQDLTALAMALGVLSFSVVAAILLMRTRIRAAANEQRLRSELRELQSEADRFRALLFVEPQILISWGAGEERPQISGDIALLLPQDAKQPLRILAFGTWLPPEPALQMDHAVDALRSAGEGFLLNLVTSAGRTVEAMGRAIGGQAIVRIRDLGGLRRDLAEMTLRHDSLLAETEMLRAFATAAPWPLWARGKDGKLSFANQAYARATEAADSADAIQRNLELLDSEDRTAMSRALNDHATFAARLPIIIGGERRMYDVHALRTGGGSAGIALDASEATALSGALARMADAHRRTLDQLSSGVAVFDGQRRLAFYNDSYRRLWDLDRPFLDGHPDDSSVLDRLRAGRKLPEQPDFRAWKARLHEAYRANEAVKDVWYLPDGRALSIVTTPNPEGGVTYLFDDVTESLDLARRYDRLINVQRETLDSLTEAVAVFGSNGRAQLFNPAFARMWKLSPEALAQQPHIDTVEAWCKPLFDDDDAWRTIHKAVTGIDNRTAVPLKLERKDGSILDCMTMPLPDGATMLTFHDITDSENVERALRERNDALEAADQMKVDFVHHVSYELRSPLTTIIGFAHLMNDPGTGPLTEKQAEYVNYITSSTDALLALTNNILDLATIDAGAMTLELGPVDVRKTIAAAAEGIQDRLTRDRIVLNVDVDPDIGDFIGDERRIIQVLYNLLANAAGFSPPDAVITVSARRTEHSVRFAVMDEGPGIPPDVRDKAFDWFESYANGSRHRGAGLGLSLVRSFVELHGGRVHFDPAIKSGTRVVCDFPLDRGAHRNAAE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWM9</accession>
    <name>DUT_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Deoxyuridine 5'-triphosphate nucleotidohydrolase</fullName>
            <shortName evidence="1">dUTPase</shortName>
            <ecNumber evidence="1">3.6.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">dUTP pyrophosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dut</name>
        <name type="ordered locus">Nwi_0044</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dUTP + H2O = diphosphate + dUMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10248"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61555"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="3.6.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the dUTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.23" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313383.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWM9"/>
    <dbReference type="SMR" id="Q3SWM9"/>
    <dbReference type="STRING" id="323098.Nwi_0044"/>
    <dbReference type="EnsemblBacteria" id="ABA03312">
        <property type="protein sequence ID" value="ABA03312"/>
        <property type="gene ID" value="Nwi_0044"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0044"/>
    <dbReference type="eggNOG" id="COG0756">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068508_1_2_5"/>
    <dbReference type="OMA" id="YAAFVHP"/>
    <dbReference type="OrthoDB" id="1669228at2"/>
    <dbReference type="UniPathway" id="UPA00610">
        <property type="reaction ID" value="UER00666"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004170">
        <property type="term" value="F:dUTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006226">
        <property type="term" value="P:dUMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046081">
        <property type="term" value="P:dUTP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00116">
        <property type="entry name" value="dUTPase_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008181">
        <property type="entry name" value="dUTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029054">
        <property type="entry name" value="dUTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11241">
        <property type="entry name" value="PTHR11241"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00692">
        <property type="entry name" value="dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="SSF51283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00576">
        <property type="entry name" value="dut"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000231414" description="Deoxyuridine 5'-triphosphate nucleotidohydrolase">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="72"/>
            <end position="74"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="89"/>
            <end position="91"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00116"/>
        </source>
    </evidence>
    <sequence length="152" mass="15826" checksum="7E61C5784214DAC2" modified="2005-10-11" version="1">MSDPIKVDVRQLPHAEGLPLPSYQSTQAAGLDLIAAIGEQAPLVLAAGRRAMVPTGLVIALPDGYEAQVRPRSGLAARHGVTVLNSPGTVDADYRGEINVLLVNLGSEAFTIRRGERIAQMIVAPVTRVELVRAAALPATPRGSGGFGSTGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMU9</accession>
    <name>Q3SMU9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Beta sliding clamp</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Nwi_0002</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of replication as well as for processivity of DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Forms a ring-shaped head-to-tail homodimer around DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the beta sliding clamp family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313341.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0002"/>
    <dbReference type="EnsemblBacteria" id="ABA03270">
        <property type="protein sequence ID" value="ABA03270"/>
        <property type="gene ID" value="Nwi_0002"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0002"/>
    <dbReference type="eggNOG" id="COG0592">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038149_4_2_5"/>
    <dbReference type="OMA" id="YLIMPVR"/>
    <dbReference type="OrthoDB" id="1040142at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009360">
        <property type="term" value="C:DNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00140">
        <property type="entry name" value="beta_clamp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001001">
        <property type="entry name" value="DNA_polIII_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022635">
        <property type="entry name" value="DNA_polIII_beta_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022637">
        <property type="entry name" value="DNA_polIII_beta_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022634">
        <property type="entry name" value="DNA_polIII_beta_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30478">
        <property type="entry name" value="PTHR30478"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00712">
        <property type="entry name" value="DNA_pol3_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02767">
        <property type="entry name" value="DNA_pol3_beta_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02768">
        <property type="entry name" value="DNA_pol3_beta_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000804">
        <property type="entry name" value="DNA_pol_III_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00480">
        <property type="entry name" value="POL3Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00663">
        <property type="entry name" value="dnan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 10" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="6 10 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA_pol3_beta" evidence="11">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="DNA_pol3_beta_2" evidence="12">
        <location>
            <begin position="131"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="DNA_pol3_beta_3" evidence="13">
        <location>
            <begin position="252"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABA03270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="372" mass="40281" checksum="A06DDAC7D757D54A" modified="2005-10-11" version="1">MKVTVERAQLLKSLGHVHRVVERRNTIPILGNVLVRAENAKLSLKATDLDLEVTETLAAETGTVGSTTVPAHMFYDIIRKLPDGAQIVLEGDGDRSVLTIRAGRSRFTLQTLPENDFPDLAAGEMTHSFTLAAADLKRLIDRTQFAISTEETRYYLNGIYLHGAGTANNATLRGVATDGHRLAQLDLALPKGAEGMPGVIIPRKTVGEVQRLIEDSEAEIGIELSQGKIRFTLGNVVLTSKLIDGTFPDYGRVIPQNNDKELVVDKKDFEAAVDRVSTISSERGRAVKLALSAGKLVLSVTNPDSGSATEELEVEYASDALDIGFNSRYLLDIAAQIEGEAAVLKLADPGSPTLVHDRDSRGALYVLMPMRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMZ6</accession>
    <name>Q3SMZ6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_3098</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06345">
        <property type="protein sequence ID" value="ABA06345"/>
        <property type="gene ID" value="Nwi_3098"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3098"/>
    <dbReference type="eggNOG" id="COG1910">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3186403_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="46" mass="5334" checksum="11D6AEBF1A4CED97" modified="2005-10-11" version="1">MVKTERVDAPDVQTMFKILRSEKFQNEFKSIGGYDISDMGKIIHET</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN21</accession>
    <name>Q3SN21_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_3072</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0382 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316236.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3072"/>
    <dbReference type="EnsemblBacteria" id="ABA06320">
        <property type="protein sequence ID" value="ABA06320"/>
        <property type="gene ID" value="Nwi_3072"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3072"/>
    <dbReference type="eggNOG" id="COG2363">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096548_2_0_5"/>
    <dbReference type="OMA" id="PTGGWAM"/>
    <dbReference type="OrthoDB" id="1985942at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006696">
        <property type="entry name" value="DUF423"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04241">
        <property type="entry name" value="DUF423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA06320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="134" mass="13724" checksum="FCCAF2534411BB1C" modified="2005-10-11" version="1">MTHLGWFRIIVIAAGLMGAAGVILGAVAAHTADGGRLALSASSMLSLHAPAAMLAVLLADRQIVRPRIGLLAAGGFVAGATLFGADMMVRQFLGHHLFPRAAPTGGTLMILSWFLLAIAAVLPRAASRSDRSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN43</accession>
    <name>Q3SN43_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter, ATPase subunit</fullName>
            <ecNumber evidence="7">3.6.3.25</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_3049</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in beta-(1--&gt;2)glucan export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.25" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316214.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3049"/>
    <dbReference type="EnsemblBacteria" id="ABA06298">
        <property type="protein sequence ID" value="ABA06298"/>
        <property type="gene ID" value="Nwi_3049"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3049"/>
    <dbReference type="eggNOG" id="COG1120">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_11_5"/>
    <dbReference type="OMA" id="KALCQEI"/>
    <dbReference type="OrthoDB" id="1752365at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="7"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="39"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA06298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="260" mass="27971" checksum="ADD45AE5D63BE8C4" modified="2005-10-11" version="1">MSEDAVLVASSLSVHLGKRLVLSDISLTLPGRRLVALVGPNGAGKTTLLRALAGLLPSEGRIEIRGDALSSLSLQERARRFAYLPQGHQVHWPLPARDVVALGRYPHGAYDPGRLTGHDADAVARAMQMADVLNLSDRRVTELSGGERSRVALARVLAVEAPVLLADEPTASLDPRYQIEVLIKLRATADNGTLVIVVTHDLGLAARFADTVLVMSEGRLVSQGPPASALSEAIMAEVFRIRTYRADHEHEIVLVPWAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SND1</accession>
    <name>Q3SND1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptide methionine sulfoxide reductase MsrB</fullName>
            <ecNumber evidence="3">1.8.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Peptide-methionine (R)-S-oxide reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">msrB</name>
        <name evidence="5" type="ordered locus">Nwi_2960</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 3">
            <text>[thioredoxin]-disulfide + H2O + L-methionyl-[protein] = [thioredoxin]-dithiol + L-methionyl-(R)-S-oxide-[protein]</text>
            <dbReference type="Rhea" id="RHEA:24164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12314"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:45764"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3">Binds 1 zinc ion per subunit. The zinc ion is important for the structural integrity of the protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MsrB Met sulfoxide reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.12" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316132.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2960"/>
    <dbReference type="EnsemblBacteria" id="ABA06210">
        <property type="protein sequence ID" value="ABA06210"/>
        <property type="gene ID" value="Nwi_2960"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2960"/>
    <dbReference type="eggNOG" id="COG0229">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031040_8_5_5"/>
    <dbReference type="OMA" id="RTEEHCA"/>
    <dbReference type="OrthoDB" id="1650261at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033743">
        <property type="term" value="F:peptide-methionine (R)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030091">
        <property type="term" value="P:protein repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="MsrB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Met_Sox_Rdtase_MsrB"/>
    </dbReference>
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        <property type="entry name" value="Met_Sox_Rdtase_MsrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10173">
        <property type="entry name" value="PTHR10173"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01641">
        <property type="entry name" value="SelR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="SSF51316"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00357">
        <property type="entry name" value="TIGR00357"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51790">
        <property type="entry name" value="MSRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MsrB" evidence="4">
        <location>
            <begin position="15"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA06210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="137" mass="15277" checksum="C16F610160553465" modified="2005-10-11" version="1">MRDSKAIPAKITKSDSEWRKELTPMQYAVLREKATEPPFSGEYEHERRGGTYVCAGCGQTLFESDAKFDSGCGWPSFTVPAVENHIDEERDISHGMVRTEVLCSRCNGHLGHVFNDGPEPTGLRYCINSAALKLEPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNE4</accession>
    <name>Q3SNE4_NITWN</name>
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        <submittedName>
            <fullName evidence="1">N-formylglutamate amidohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2947</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2947"/>
    <dbReference type="EnsemblBacteria" id="ABA06197">
        <property type="protein sequence ID" value="ABA06197"/>
        <property type="gene ID" value="Nwi_2947"/>
    </dbReference>
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    <dbReference type="OMA" id="NHPYKGV"/>
    <dbReference type="OrthoDB" id="813848at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007709">
        <property type="entry name" value="N-FG_amidohydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05013">
        <property type="entry name" value="FGase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="295" mass="32954" checksum="C458E72B8A618755" modified="2005-10-11" version="1">MSQFDGDQPPPFEIAEPVTWRSPIIFNSPHSGSIYPEQFLSASRIDLAGLRRSEDSFMDELIGGLTDAGFPVVRVNFPRSYVDVNREPYELDPRMFTGRLPSFANTRSMRVAGGLGTIPRVVGDGEEIYRERLSVEDALGRIEQLYKPYHRALRRLIYKAHQTFGTVILVDCHSMPSTGIAPEEPRRSDIVIGDRYETSCAPQIPNIIDDLLRSLGYSVTRNKPYAGGFITEHYGNPASGLHAVQLELNRAVYMDERHCERGPRFNQVAADFLTLANALAAIPLEDLGPFRAAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNF3</accession>
    <name>Q3SNF3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2938</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2938"/>
    <dbReference type="EnsemblBacteria" id="ABA06188">
        <property type="protein sequence ID" value="ABA06188"/>
        <property type="gene ID" value="Nwi_2938"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2938"/>
    <dbReference type="eggNOG" id="COG0071">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2524189_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="84" mass="9527" checksum="7E31BCD982E654EC" modified="2005-10-11" version="1">MVSKGRQLFGALVSMLLHKEDMAMRNTFDFAPLFRSTIGFDRMLDTLESASRVTSIDNVATLRHRQDERGRLPHQHGGGRLDRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="126" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNH7</accession>
    <name>Q3SNH7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_2914</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316094.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2914"/>
    <dbReference type="EnsemblBacteria" id="ABA06164">
        <property type="protein sequence ID" value="ABA06164"/>
        <property type="gene ID" value="Nwi_2914"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2914"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_37_5"/>
    <dbReference type="OMA" id="HEVDQRD"/>
    <dbReference type="OrthoDB" id="692375at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="8">
        <location>
            <begin position="190"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="251"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA06164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="456" mass="49820" checksum="222025F8F00CBF0D" modified="2005-10-11" version="1">MPLRRSILSRIVVLHVVAIIVAALVLRSILHWSLASDVAELQLDKMATQIELFARHLKPSSAGGWSLNLPDGTREQYLEAYGRYSYAIIDDAGSVILASSPDKTPLFAIDSSAKGIVPHNTRTEGGARTIAGASARRVIDGHPVWIQVAEDLSHSDVVVDDVLTNFLRRVVWIVVPVLLLLLAADIVIFLLAIRPLHRASGRARDISPTRLDVRLPTDDMPSEILPLVEAVNQALDRLEHGFLAQREFAADAAHELRTPLAILRTRIETLPLNDSIHDLARDVENMSRVVSQLLDATELETAVVDPDAVADIHGVCVEVAEFIAPLALKQGKSIELMCSEESVLVKGNAEMIRRATRNLVENALHHTPEDTTIQIVISADGSISVIDNGDGVPVADRKLVFQRFWRRSPRTTGGAGLGLSIVEKIVEAHQGSIAVDDAPGRGARFTMRFRRDQGTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNJ6</accession>
    <name>Q3SNJ6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase-like protein</fullName>
            <ecNumber evidence="2">3.1.2.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2895</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.2.6" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316075.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2895"/>
    <dbReference type="EnsemblBacteria" id="ABA06145">
        <property type="protein sequence ID" value="ABA06145"/>
        <property type="gene ID" value="Nwi_2895"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2895"/>
    <dbReference type="eggNOG" id="COG0491">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0607">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_7_1_5"/>
    <dbReference type="OMA" id="DYKGDTV"/>
    <dbReference type="OrthoDB" id="2056793at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004416">
        <property type="term" value="F:hydroxyacylglutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050313">
        <property type="term" value="F:sulfur dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07724">
        <property type="entry name" value="POD-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044528">
        <property type="entry name" value="POD-like_MBL-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="SSF52821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="248"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="346" mass="38081" checksum="9956C8990ACD57BF" modified="2005-10-11" version="1">MIFRQLFDSVSGTYSYLMASRAGGEALIIDPVLEKVDRYCQLLRELNLRLVKAIDTHLHADHITGLGELRDRTQCVTVMGEQTRADVVSMRVSDGDKVNIEGIGLDVMYTPGHTDDSYSYLMGDRVFTGDTLLIRGTGRTDFQNGSARAQYDSIFNRLLKLPDETMVFPAHDYKGDTVSTIGEERRHNPRLQVRSIDEYVELMDNLKLPNPKLMDVVIPANMHVGLRQDDLAKQGLAMTPREAIARLGQPDILPVDLREAHERARHGTLAGALHAPYPDIADSLKPGGMLREVAAATGRRIVFFCAFGERSAMAVTAAKDAGLTNAVHIEGGIDAWKRAGGPVVAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNK3</accession>
    <name>Q3SNK3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Ribonucleoside-diphosphate reductase</fullName>
            <ecNumber evidence="4 12">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Nwi_2888</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>[thioredoxin]-disulfide + a 2'-deoxyribonucleoside 5'-diphosphate + H2O = [thioredoxin]-dithiol + a ribonucleoside 5'-diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23252"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="EC" id="1.17.4.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Genetic information processing; DNA replication.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the ribonucleoside diphosphate reductase large chain family.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="4 12"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316068.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2888"/>
    <dbReference type="EnsemblBacteria" id="ABA06138">
        <property type="protein sequence ID" value="ABA06138"/>
        <property type="gene ID" value="Nwi_2888"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2888"/>
    <dbReference type="eggNOG" id="COG0209">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000404_3_0_5"/>
    <dbReference type="OMA" id="RGSIQNI"/>
    <dbReference type="OrthoDB" id="357568at2"/>
    <dbReference type="UniPathway" id="UPA00326"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013346">
        <property type="entry name" value="NrdE_NrdA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000788">
        <property type="entry name" value="RNR_lg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013509">
        <property type="entry name" value="RNR_lsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008926">
        <property type="entry name" value="RNR_R1-su_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039718">
        <property type="entry name" value="Rrm1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11573">
        <property type="entry name" value="PTHR11573"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02867">
        <property type="entry name" value="Ribonuc_red_lgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00317">
        <property type="entry name" value="Ribonuc_red_lgN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01183">
        <property type="entry name" value="RIBORDTASEM1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48168">
        <property type="entry name" value="SSF48168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02506">
        <property type="entry name" value="NrdE_NrdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00089">
        <property type="entry name" value="RIBORED_LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 12 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-cone" evidence="13">
        <location>
            <begin position="31"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-cone" evidence="13">
        <location>
            <begin position="151"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABA06138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="969" mass="107121" checksum="B797E51826B2A736" modified="2005-10-11" version="1">MSAATETVPAARIVRPSLSAAATAPSSEPACQVIRRNGSVSPFDSAKIAVALTKAFLAVEGNSAAASRRVHDIVTGLTDQIVASLTRRSDAGRTFHIEDIQDQVELALMRSEHHKVARAYVLYREERARERAKAQESKSKKGGAAAAKAALRMKSADGSLIALDTPRLTAVVEEACAGLGDVSASAILTETHRNLYDGISVDELAQAPILAARTLVETDPDYAKVSARLLLDKMRREALSFVAGRLEQATQAEMAERYADYFLSYVRAGIDHELLDPEIGRFDLDRLAAALKPERDLRFDYLGLQTLYDRYFLHVRSVRFELPQAFFMRVACGLALREIDREEKAIAFYDLLSSFDFMASTPTLFNAGTLRPQLSSCFLTTVADDLDGIFKAIKDNALLSKYAGGLGNDWTRVRGLGAHIKGTNGESQGVVPFLKVANDTAIAVNQGGKRKGAVCAYLETWHIDIEEFLDLRKNTGDDRRRTHDMNTANWVPDLFMQRVEEDGMWTLFSPDETPDLHDLTGKAFAGRYAYYEEKAARGELRVTKQVRAVDLWRRMLTMLFETGHPWITFKDPCNLRSPQQHCGVVHSSNLCTEITLNTSNTEVAVCNLGSVNLVNHIKDGKLDHEHLARTVRIAMRMLDNVLDVNFYTIPEARTSNLRHRPVGLGIMGFQDALNILRLPYASDEAVSFADESMEAIAYHAISGSVELAAERGRYPSYQGSLWSKGVLPLDSLELVDEARGAEVDIDRSSRLDWDSLRQRVMTIGMRNSNCMAIAPTATISNICGVSQSIEPAYQNLYVKSNMSGDFTVVNAHLVRDLKARGLWDEVMISDLKYFDGSVGEIDRVPDDLKALYATAFEIDSAWLIDAAARRQKWIDQAQSLNLYIANPSGKKLDALYRHAWKRGLKTTYYLRSRSATHVEKSTLKGTDGKLNAVAASVSVAPLNGHAAVASASEGWKSCRVDDPTCEACQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNP2</accession>
    <name>Q3SNP2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DDE_3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2849</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06099">
        <property type="protein sequence ID" value="ABA06099"/>
        <property type="gene ID" value="Nwi_2849"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2849"/>
    <dbReference type="eggNOG" id="COG3335">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1979200_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0814">Transposable element</keyword>
    <feature type="domain" description="DDE_3" evidence="2">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="126" mass="14377" checksum="1EC48D2818D129C7" modified="2005-10-11" version="1">MNGEIFRVYLQRCLVPTLKPGDIVVMDNLPAHKNDDVRQIIEAAGAQLRYLPPYSPDLNPIEMAFAKLKAHLRKAAERSIPALWNRIGSILDLLSRPMHQLLQSRRLCVISIQNGSSRHFLRHFSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNP8</accession>
    <name>Q3SNP8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Resolvase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2841</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2841"/>
    <dbReference type="EnsemblBacteria" id="ABA06093">
        <property type="protein sequence ID" value="ABA06093"/>
        <property type="gene ID" value="Nwi_2841"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2841"/>
    <dbReference type="eggNOG" id="COG1961">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010686_0_2_5"/>
    <dbReference type="OMA" id="HRNSRNF"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038109">
        <property type="entry name" value="DNA_bind_recomb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011109">
        <property type="entry name" value="DNA_bind_recombinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07508">
        <property type="entry name" value="Recombinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="SSF53041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51737">
        <property type="entry name" value="RECOMBINASE_DNA_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="1">
        <location>
            <begin position="3"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Recombinase" evidence="2">
        <location>
            <begin position="151"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="336" mass="37419" checksum="BECBDFC11E39FF38" modified="2005-10-11" version="1">MIRIALYARYSSENQRDASIEDQLRQCRERAEREGWTVVETYSDRAISGSSMFRAGIRSLLADAQAGRFDMVLSEALDRISRDQEDVAAVFKRLRFAGVSIVTLSEGEINELHVGLKGTMNALFIKDLAIKTHRGQRGRVEAGKSGSGRAAYGYRVVHQLDASGEPIRGERAIVEDEAEVVRRIFREYASGRSPKEIAFQLNREGVPGLRNRAWIDATIRGNPLAGTGILNNELYAGVLAWNRQRFINNPETGTRVSRINPESAWIRTEVPHLRIVNDALWQAVRERQRGISALFGPNLANTREGRAKRLYLTNRPSPFFPACLPAAAAAAGSAWS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNT5</accession>
    <name>ASSY_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Argininosuccinate synthase</fullName>
            <ecNumber evidence="1">6.3.4.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Citrulline--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argG</name>
        <name type="ordered locus">Nwi_2803</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-aspartate + L-citrulline = 2-(N(omega)-L-arginino)succinate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10932"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the argininosuccinate synthase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.5" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316001.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SNT5"/>
    <dbReference type="SMR" id="Q3SNT5"/>
    <dbReference type="STRING" id="323098.Nwi_2803"/>
    <dbReference type="EnsemblBacteria" id="ABA06056">
        <property type="protein sequence ID" value="ABA06056"/>
        <property type="gene ID" value="Nwi_2803"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2803"/>
    <dbReference type="eggNOG" id="COG0137">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032784_4_2_5"/>
    <dbReference type="OMA" id="QCEVVTF"/>
    <dbReference type="OrthoDB" id="357142at2"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00113"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004055">
        <property type="term" value="F:argininosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01999">
        <property type="entry name" value="Argininosuccinate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00005">
        <property type="entry name" value="Arg_succ_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001518">
        <property type="entry name" value="Arginosuc_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018223">
        <property type="entry name" value="Arginosuc_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023434">
        <property type="entry name" value="Arginosuc_synth_type_1_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024074">
        <property type="entry name" value="AS_cat/multimer_dom_body"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11587">
        <property type="entry name" value="PTHR11587"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00764">
        <property type="entry name" value="Arginosuc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69864">
        <property type="entry name" value="SSF69864"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00032">
        <property type="entry name" value="argG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00564">
        <property type="entry name" value="ARGININOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00565">
        <property type="entry name" value="ARGININOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000263943" description="Argininosuccinate synthase">
        <location>
            <begin position="1"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="11"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00005"/>
        </source>
    </evidence>
    <sequence length="409" mass="45626" checksum="9CD3751D7268B4DD" modified="2005-10-11" version="1">MSKKVQKVVLAYSGGLDTSIILKWLQTTYGAEVVTFTADLGQGEELEPARRKAQLLGIKDENIFIEDLREEFVRDYVFPMFRANAVYEGQYLLGTSIARPLIAKKQIEIAEKVGADAVAHGATGKGNDQVRFELGYYALKPDITIIAPWREWDLRSREQLIAFAEQHQIPIAKDKRGEAPFSVDANLLHASSEGKVLEDPAQEVPDYVYSRTIDPEAAPDQPTYITIDFEQGDAVAIDGGKMSPATLLAKLNELGRANGIGRLDLVENRFVGMKSRGMYETPGGAILLVAHRGIEQITLDRGAAHLKDELMPKYAELVYNGFWFSPEREMLQAAIDQSQRYVTGQVRLKLYKGNVILVGRDSAYSLYDQDLVTFEEGAVAYDHRDAAGFIKLNALRLRTLGQRRKKLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNU1</accession>
    <name>Q3SNU1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">AMP-dependent synthetase and ligase</fullName>
            <ecNumber evidence="4">6.2.1.26</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2797</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.26" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315995.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2797"/>
    <dbReference type="EnsemblBacteria" id="ABA06050">
        <property type="protein sequence ID" value="ABA06050"/>
        <property type="gene ID" value="Nwi_2797"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2797"/>
    <dbReference type="eggNOG" id="COG0318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_0_5"/>
    <dbReference type="OMA" id="LGCITHG"/>
    <dbReference type="OrthoDB" id="961884at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008756">
        <property type="term" value="F:o-succinylbenzoate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="2">
        <location>
            <begin position="25"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="3">
        <location>
            <begin position="413"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA06050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="518" mass="55998" checksum="F788DCEA197FC86E" modified="2005-10-11" version="1">MNANLFSRLFDDLDDASLLAIETMDGPRITYGDLIAFSGRLANVLVARGVRPGDRVAVQAEKSVPNLALYLATARAGAVYLPLNDAYTLSELDYFITDAEPSLVVCDPSKAGGLQAIASKVNATIETLDAKGKGSLTGAATTAKPDFITVARAGDDLAAILYTSGTTGRSKGAMLTHDNLTSNAMSLVDAWRFTQKDVLIHALPIHHTHGLFVAGNVTLLARASMIFLPRFDPEAVINIMARATVLMGVPTFYTRLLQHPALTKESTGPMRLFISGSAPLLADTHREWSARTGHALLERYGMTETGMNTSNPYDGDRVPGAVGPALRGVSVRVTDRESGKELPRESVGMIEVRGPNVFKGYWRMPEKTRTEFRDDGFFITGDIGKIDHRDYVHILGRGTDLVITGGLNVYPKEVESEIDALAGVAESAVIGVPHPDFGEGVTAVVVRGKNGDMTEEQMLKALDGRLAKFKIPKRIIFVDELPRNAMGKVQKNILRHTYAGLYGSKVPGGFLPHDVKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNZ2</accession>
    <name>Q3SNZ2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="14">ABC transporter, ATP-binding/permease protein</fullName>
            <ecNumber evidence="14">3.6.3.25</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Nwi_2746</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in beta-(1--&gt;2)glucan export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.25" evidence="14"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315944.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2746"/>
    <dbReference type="EnsemblBacteria" id="ABA05999">
        <property type="protein sequence ID" value="ABA05999"/>
        <property type="gene ID" value="Nwi_2746"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2746"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_3_5"/>
    <dbReference type="OMA" id="RVMHDLR"/>
    <dbReference type="OrthoDB" id="643917at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011918">
        <property type="entry name" value="ABC_MsbA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02204">
        <property type="entry name" value="MsbA_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="186"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="290"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="69"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="386"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="421"/>
            <end position="428"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABA05999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="627" mass="66774" checksum="9D115490401A4DA4" modified="2005-10-11" version="1">MSSVEVRENTSKAPAVALPPGSSPKAGTGASEPAAPDQAAPSAKDARRLRLRPLLALTPYILRYRGRALLALVALIVAALTTLVVPLAVRRIIDFGFTAEGVAMINSYFTMMIGVVAVLAGASAARYFLVTTTGERIVADLRRDVFAHLISLSPVFFDTARSGELISRLTADTTQIKSAVGSSISVALRNVVLFIGAASMMVITSPRLSGLVLAAIPLIIVPLVAFGRWVRRLSRSAQDNLADATAYASELVGGIRTVQAYTNERLADARFGGEVEHAYLAARNSARARAVLTAIIIFIVFTSVVLILWIGSRDVLEGDMTPGRLGQFVLYAAFAATGLGQLSEVWGEVAAASGAAERLFELLRVKPVIAAPPTPRALPAPARGDVTLDNVSFAYPSRPEVKVLDGVSLAVKAGEKVAIVGPSGAGKSTIFHLLLRFYDPVSGTISFDGVPLRTADPREVRARIALVPQESVVFAASARENIRFGRPDASDAEVERAAELAHATEFIRRLPGGFEALLGERGVTLSGGQRQRIAIARAILRDAPLLLLDEATSSLDAESETLVQTALEELMRHRTTLVIAHRLATVLSCDRIMVMERGRIVEQGTHASLVAANGLYARLARLQFEGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP61</accession>
    <name>Q3SP61_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2677</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2677"/>
    <dbReference type="EnsemblBacteria" id="ABA05930">
        <property type="protein sequence ID" value="ABA05930"/>
        <property type="gene ID" value="Nwi_2677"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2677"/>
    <dbReference type="eggNOG" id="ENOG5031195">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_173328_0_0_5"/>
    <dbReference type="OMA" id="HRHPLCR"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="102" mass="11111" checksum="BF2E61D08EA7242A" modified="2005-10-11" version="1">MPVNGMHLPRERPGTVGRGKKAMECDIGPRKIPATIQELQARVAIAFGRHPLCREVRFDVVSTPKTRSGANWTVSMSAVPPDAIWEASELVADIQDAYTLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPC3</accession>
    <name>Q3SPC3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phospholipid-binding protein, PBP family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2615</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2615"/>
    <dbReference type="EnsemblBacteria" id="ABA05868">
        <property type="protein sequence ID" value="ABA05868"/>
        <property type="gene ID" value="Nwi_2615"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2615"/>
    <dbReference type="eggNOG" id="COG1881">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083918_2_1_5"/>
    <dbReference type="OMA" id="LAWDEVP"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00865">
        <property type="entry name" value="PEBP_bact_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005247">
        <property type="entry name" value="YbhB_YbcL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30289">
        <property type="entry name" value="PTHR30289"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="SSF49777"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00481">
        <property type="entry name" value="TIGR00481"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="247" mass="26605" checksum="3FEA17DD881324E9" modified="2005-10-11" version="1">MSRAFTGDAPLWDRILLHPDPTQVCQYRDAPLKHFRFRLNGSGSSGIVVLTLSARKAEYPSSGQARGNAWLENATIRHRENIMAFKVVSDSFKDGDYLSTDHVVSADFGFGCAGGNRSPHLRWSGAPEGTKSFAVTCFDPDAPTGSGFWHWVVINIPASVSELELDAGNPDSGKLPAGALQTRTDYGAARYGGPCPPEGDHPHRYVFTVHAVGGDINADADTSAAVIGFQLHFNTLAKSAVMGLFKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPC9</accession>
    <name>Q3SPC9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2609</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315812.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2609"/>
    <dbReference type="EnsemblBacteria" id="ABA05862">
        <property type="protein sequence ID" value="ABA05862"/>
        <property type="gene ID" value="Nwi_2609"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2609"/>
    <dbReference type="eggNOG" id="COG5459">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072591_0_0_5"/>
    <dbReference type="OMA" id="DWCHFVQ"/>
    <dbReference type="OrthoDB" id="1345405at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015324">
        <property type="entry name" value="Ribosomal_Rsm22-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09243">
        <property type="entry name" value="Rsm22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="348" mass="37553" checksum="E82270FCE8FDE335" modified="2005-10-11" version="1">MIPPDLPAELKAALQRKAQGLSRNDAAARAAAISQTYRSGGGSNTIRNESDALAYALARMPATYAAVVASLNALRETRPKFTPLSLLDIGAGPGTATWAAAQAFESLDAFSAIDSNPALRSLALDLVRDDLRLAGLRYAQREASAGLRDAPESADLVIASYVLGEMRMERQAALAELMWAATRDTLVVVEPGTPAGYQRILGLRRQLIAQGAHVIAPCPHDGECPLAAFPDETGAASPRGNASRQNATPDWCHFVQRLPRSRAHKHIKGAELPFEDEKFSYVVLARTPDRPRPARVLAPPMLTKAAMRLKLCRPDGSVGVTTFLRRDKASYARLRRIGWGDAVNLTET</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPF8</accession>
    <name>Q3SPF8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FAD linked oxidase</fullName>
            <ecNumber evidence="2">1.1.2.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.2.4" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315784.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2580"/>
    <dbReference type="EnsemblBacteria" id="ABA05833">
        <property type="protein sequence ID" value="ABA05833"/>
        <property type="gene ID" value="Nwi_2580"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2580"/>
    <dbReference type="eggNOG" id="COG0277">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017779_0_0_5"/>
    <dbReference type="OMA" id="PLNASCW"/>
    <dbReference type="OrthoDB" id="295054at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004458">
        <property type="term" value="F:D-lactate dehydrogenase (cytochrome) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="SSF55103"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="416" mass="42862" checksum="551269DC28D568CA" modified="2005-10-11" version="1">MDTLKAGDAADVEDAVRAAVAGDQPLEIIGHGSKRSIGHAMTTDAVLDVSALNAVTCYEPHELIITVQAGAPLDEVVSLLDSRNQEFAFEPMDTAALLGVRSGRGTIGGMIGAGLAGPRRIKAGGVRDHLLGAHAVSGFGDGFKAGGRVVKNVTGYDLCKLLAGSWGTLAVMTEVTLKVMPKAEAERTLVLRGLDDVTANRAMTAALGSPYDVSGAAHMSRTALEAARGALAVLASTGQAATLLRLEGIAASVAHRGAALAAHLAPFGAGELVEGAESAALWCDVRDVAPFAADGPLGGWPVWRIVCPPASGGAFGQALARETGGEVIYDWAGGLLWAALPSSVDVVDAYAASLRRRLAPLGGHATLLRAGEELRRTVDVFHPQAKGVAALAERVRRSFDPGSILNRGRLTRTPIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPL6</accession>
    <name>Q3SPL6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">[LSU ribosomal protein L3P]-glutamine N5-methyltransferase</fullName>
            <ecNumber evidence="6">2.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_2522</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315726.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2522"/>
    <dbReference type="EnsemblBacteria" id="ABA05775">
        <property type="protein sequence ID" value="ABA05775"/>
        <property type="gene ID" value="Nwi_2522"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2522"/>
    <dbReference type="eggNOG" id="COG2890">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018398_5_1_5"/>
    <dbReference type="OMA" id="IYYGHGT"/>
    <dbReference type="OrthoDB" id="1816476at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009007">
        <property type="term" value="F:site-specific DNA-methyltransferase (adenine-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004556">
        <property type="entry name" value="HemK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017127">
        <property type="entry name" value="Ribosome_L3_Gln-N5_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037167">
        <property type="entry name" value="Mtase_YfcB_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00536">
        <property type="entry name" value="hemK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03533">
        <property type="entry name" value="L3_gln_methyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MTS" evidence="5">
        <location>
            <begin position="170"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="340" mass="36607" checksum="3D6A0D843A4D84D8" modified="2005-10-11" version="1">MAKTAARGGRSKAATSGSGRKRPTARVAAKAANSAVAGTSAPELETIFDYVRHAADRLQRAHVIFAHGTTDPVAEAAFLVGEAMQLHPDEVEPQATLRVPKAAGRRILAWIEQRIATRKPAAYLVNRMYMRGLPFYVDERVIVPRSFIGEILDSHFGGDDVLCLRDPATLTRVLDLCTGSGCLAILAARAFPNATVDATDLSADALAVAARNVREHGLDDRIQLAQGDLFAAVRGRRYDLIISNPPYVDAEGMAGLPRECLVEPRMAFDGGTEGIDLVRRIIDRAETHLAPGGGLLCEVGRCKPAIEKAYPRADLLWLDTEESEGEVFWIDAAGLDGLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPQ6</accession>
    <name>Q3SPQ6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_2482</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2482"/>
    <dbReference type="EnsemblBacteria" id="ABA05735">
        <property type="protein sequence ID" value="ABA05735"/>
        <property type="gene ID" value="Nwi_2482"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2482"/>
    <dbReference type="eggNOG" id="COG0628">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021513_0_0_5"/>
    <dbReference type="OMA" id="VIEPWLY"/>
    <dbReference type="OrthoDB" id="944044at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002549">
        <property type="entry name" value="AI-2E-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716">
        <property type="entry name" value="PTHR21716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01594">
        <property type="entry name" value="AI-2E_transport"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="9"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="234"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="333"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="126"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="638"/>
            <end position="662"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA05735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="662" mass="71312" checksum="F6111807FA052F98" modified="2005-10-11" version="1">MAGGGFGDRALASAVIAALVICGLYVGREIFVPIVLAVLLSFVLAPLVDILERWHFPRFASVPVVVLLAFIAIFALGGLIVREVRGLADSLPSYQQTMQQKIQSLRALTATGPLDRAAEILQNLGKEISGPQSPPPPAEISPSSRTAEPVKPIPVEVRTPPQSALENISALISPLLHPLATIGIVVVFVIFILFQREDLRNRFIKLAGSNDLQSATAAIDDAAGRLSRLLLMQVLLNTGFGVIVASGLLLIGVPSAILWGILAAIMRFVPYVGPFIAAFFPLTLAVAVDPGWSMLLWSGALILLTELIVGQVFDPLLVGHSSGLSPVAVVVSATFWTALWGPIGLVLATPLTICLVVLGRHVEQLRFLDILLGDRPALSPPELFYQRMLADDPAEAVEMAEKFLKERSLAEYYENVALKGLMLAQADLKRERLLPDRVAEIRDSVAEVIEGLASEFDDVLGNAQTKEDDDASDVTADAPMPELPFIIREGLPADWQADHPVLCVAERSDLDQAAAMFLAEILSKHGLGARAASKEETSTSGIFHLEASGVMLVCISTLDDGSPAHTRHLVRKLRRKMPQARILVCCWGCTNSGMAREHLKADAVAENLGEAAAYCLSLVDQTVAQDGVHETGVIPRVRHQPLPVARQKQPAAEGRHGMNDRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPR9</accession>
    <name>Q3SPR9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2469</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2469"/>
    <dbReference type="EnsemblBacteria" id="ABA05722">
        <property type="protein sequence ID" value="ABA05722"/>
        <property type="gene ID" value="Nwi_2469"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2469"/>
    <dbReference type="eggNOG" id="ENOG502ZCMG">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070564_0_0_5"/>
    <dbReference type="OMA" id="EGRTREY"/>
    <dbReference type="OrthoDB" id="668757at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024291">
        <property type="entry name" value="DUF3829"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12889">
        <property type="entry name" value="DUF3829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="354" mass="39105" checksum="088A9CDF1837C96C" modified="2005-10-11" version="1">MHGGSREAVTHPVALLRMNLCRASRDQRWDLSASRGKRTMTPFRAIALLSAAAFGLSLAPAAAQDKTPPSSSPSPLVGKLNAYVGCINRLSERSYESRNRYFSWAKKTGPTGKERIIYGTYTIYDTTDCKKNVEMANASEPHDAELESAASAYAEAVGKLEPLLKEADEYYQQENYKDDKMAKGKEMHPRLVAAWDAFASADKKLRAGVEAIKDKQAEEQLAAIEAKEGRKTHYYAEALMIHAKRLLRAEDTSKPDIAAITKALTEYETTVKAAEDASGKDGDTKLGSSFLSSAKSYLTSAKQLMRRIRDKVPYSQGDRMMLGSGGGWMVEGSPPRLLRDYNQLIESYNRGARI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPS5</accession>
    <name>Q3SPS5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Lytic transglycosylase, catalytic</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2463</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the virb1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2463"/>
    <dbReference type="CAZy" id="GH23">
        <property type="family name" value="Glycoside Hydrolase Family 23"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05716">
        <property type="protein sequence ID" value="ABA05716"/>
        <property type="gene ID" value="Nwi_2463"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2463"/>
    <dbReference type="eggNOG" id="COG0741">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114428_0_0_5"/>
    <dbReference type="OMA" id="ESTHRPW"/>
    <dbReference type="OrthoDB" id="1444566at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008258">
        <property type="entry name" value="Transglycosylase_SLT_dom_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01464">
        <property type="entry name" value="SLT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229107" evidence="2">
        <location>
            <begin position="25"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="SLT" evidence="3">
        <location>
            <begin position="33"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="158" mass="17479" checksum="797CCAC3DF009447" modified="2005-10-11" version="1">MTNFSKFGPLLVFVAMAWPQPSTAQRAEYTTMVTAHAQANGVPVDLVHRVIMRESRYNPRAVHRGNYGMMQIKLATARGLGYTGTAEGLLDPETNLTYAVKYLAGAYRLANGNHDRAVAYYAGGYYYAAKRQRLQRARHETMSADARLPGAPLSIAPR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-03-24" modified="2022-05-25" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPT8</accession>
    <name>GREA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription elongation factor GreA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Transcript cleavage factor GreA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">greA</name>
        <name type="ordered locus">Nwi_2450</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GreA/GreB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315658.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SPT8"/>
    <dbReference type="SMR" id="Q3SPT8"/>
    <dbReference type="STRING" id="323098.Nwi_2450"/>
    <dbReference type="EnsemblBacteria" id="ABA05703">
        <property type="protein sequence ID" value="ABA05703"/>
        <property type="gene ID" value="Nwi_2450"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2450"/>
    <dbReference type="eggNOG" id="COG0782">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101379_2_0_5"/>
    <dbReference type="OMA" id="TWLTQEA"/>
    <dbReference type="OrthoDB" id="1536415at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070063">
        <property type="term" value="F:RNA polymerase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032784">
        <property type="term" value="P:regulation of DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00105">
        <property type="entry name" value="GreA_GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036953">
        <property type="entry name" value="GreA/GreB_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018151">
        <property type="entry name" value="TF_GreA/GreB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006359">
        <property type="entry name" value="Tscrpt_elong_fac_GreA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028624">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001437">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023459">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022691">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036805">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30437">
        <property type="entry name" value="PTHR30437"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01272">
        <property type="entry name" value="GreA_GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03449">
        <property type="entry name" value="GreA_GreB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006092">
        <property type="entry name" value="GreA_GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46557">
        <property type="entry name" value="SSF46557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01462">
        <property type="entry name" value="greA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00829">
        <property type="entry name" value="GREAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_1000094179" description="Transcription elongation factor GreA">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="47"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00105"/>
        </source>
    </evidence>
    <sequence length="158" mass="17167" checksum="49CB8D42B9DE8B36" modified="2005-10-11" version="1">MVEKVPMTRGGHAALADELKKRQSVDRPRIIEQIAEARAHGDLSENAEYHAAKEEQSHNEGRIAELEDKLARADIIDVSKLSGDTIKFGATVTLIDEDTDKKAVWQIVGEAEADAKQGRISITSPLARALIGKKKGSSVEVVAPGGAKAYEIAKVEWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPY7</accession>
    <name>Q3SPY7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase FkbM</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315615.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2400"/>
    <dbReference type="EnsemblBacteria" id="ABA05654">
        <property type="protein sequence ID" value="ABA05654"/>
        <property type="gene ID" value="Nwi_2400"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2400"/>
    <dbReference type="eggNOG" id="COG2242">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068034_1_0_5"/>
    <dbReference type="OMA" id="NEMETAS"/>
    <dbReference type="OrthoDB" id="1340956at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006342">
        <property type="entry name" value="FkbM_mtfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05050">
        <property type="entry name" value="Methyltransf_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01444">
        <property type="entry name" value="fkbM_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_21" evidence="1">
        <location>
            <begin position="72"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="267" mass="29411" checksum="80E3070D1D9DDDAF" modified="2005-10-11" version="1">MKTFISAALRGINRAIAPFGVRLMRNNAPTRSFALFFAHLRSLGFECKTVIDVGVAFGTPPIYDAFPDAKYFLVEPVAECRSVLEELKGRLNAEYFLVAAGATNGEVTFNVHDDISGSSLFAQVEGKALDGEARLTPIRRLDSLLPEKLEHPVLLKIDTQGAEIEVLKGLGDRISAIDLLIVETSMLPMRQGIPEFAEIVRFCDEAGFAVYDVLEGHTRVLDGALAQIDLALVRKDSILRKQSAFFTEKQAADYLNRPSKLRRAKVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ05</accession>
    <name>Q3SQ05_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Formyltetrahydrofolate deformylase</fullName>
            <ecNumber evidence="3">3.5.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Formyl-FH(4) hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">purU</name>
        <name evidence="5" type="ordered locus">Nwi_2382</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the hydrolysis of 10-formyltetrahydrofolate (formyl-FH4) to formate and tetrahydrofolate (FH4).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(6S)-10-formyltetrahydrofolate + H2O = (6S)-5,6,7,8-tetrahydrofolate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19833"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
            <dbReference type="EC" id="3.5.1.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; IMP biosynthesis via de novo pathway; formate from 10-formyl-5,6,7,8-tetrahydrofolate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PurU family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.10" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315597.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2382"/>
    <dbReference type="EnsemblBacteria" id="ABA05636">
        <property type="protein sequence ID" value="ABA05636"/>
        <property type="gene ID" value="Nwi_2382"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2382"/>
    <dbReference type="eggNOG" id="COG0788">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038395_3_0_5"/>
    <dbReference type="OMA" id="RFMQVLP"/>
    <dbReference type="OrthoDB" id="979667at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00170"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008864">
        <property type="term" value="F:formyltetrahydrofolate deformylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016742">
        <property type="term" value="F:hydroxymethyl-, formyl- and related transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04875">
        <property type="entry name" value="ACT_F4HF-DF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08648">
        <property type="entry name" value="FMT_core_Formyl-FH4-Hydrolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01927">
        <property type="entry name" value="PurU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041729">
        <property type="entry name" value="Formyl-FH4-Hydrolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004810">
        <property type="entry name" value="PurU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044074">
        <property type="entry name" value="PurU_ACT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42706">
        <property type="entry name" value="PTHR42706"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01575">
        <property type="entry name" value="FFH4HYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="SSF53328"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00655">
        <property type="entry name" value="PurU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="3" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="4">
        <location>
            <begin position="7"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="285" mass="32420" checksum="4D1A33C45D419D4C" modified="2005-10-11" version="1">MSHHQYVLTLSCPDRPGIVSAVSTFLAHNGQNIIDAQQFDDAETGRFFMRVVFNAADLAVRLPALQTGFDAIAERFRMTWHMRDRAAQRKVMLLVSKSDHCLVDILYRWRTGELKMIPTAIVSNHPRETYAHLDFGEIPFHYFPVTSKASQEAQVLKLVEETGTDLVVLARYMQILSNDMSARLSGRCINIHHSFLPGFKGAKAYHQAHERGVKLIGATAHYVTSDLDEGPIIDQDVERISHRDTPEALVRKGRDIERRVLARAIRHHLDDRVILNGRKTVVFMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ33</accession>
    <name>Q3SQ33_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2354</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2354"/>
    <dbReference type="EnsemblBacteria" id="ABA05608">
        <property type="protein sequence ID" value="ABA05608"/>
        <property type="gene ID" value="Nwi_2354"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2354"/>
    <dbReference type="eggNOG" id="ENOG50339KS">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_187649_1_0_5"/>
    <dbReference type="OMA" id="FGRTKSQ"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025227">
        <property type="entry name" value="DUF4169"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13770">
        <property type="entry name" value="DUF4169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="64" mass="7305" checksum="DAD7569F827F5F61" modified="2005-10-11" version="1">MVMGDVINLNKRRKRAERERAAAVADANRARFGLTKAERDLSKQRKARELSLLDQHRIDGEDAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ48</accession>
    <name>Q3SQ48_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter, ATPase subunit</fullName>
            <ecNumber evidence="7">3.6.3.25</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2339</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in beta-(1--&gt;2)glucan export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.25" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315557.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2339"/>
    <dbReference type="EnsemblBacteria" id="ABA05593">
        <property type="protein sequence ID" value="ABA05593"/>
        <property type="gene ID" value="Nwi_2339"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2339"/>
    <dbReference type="eggNOG" id="COG1125">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_5"/>
    <dbReference type="OMA" id="DKICIMN"/>
    <dbReference type="OrthoDB" id="1220708at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="8"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="42"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="263" mass="28085" checksum="4E83724EDC0C850D" modified="2005-10-11" version="1">MGETSPLIAFSHVVKSFNGSHVRAVNDVSFDIAAGEFLAVVGGSGSGKTTLLRLANRLIDPDAGTITVAGEDIAGIDAIGLRRRIGYVFQSGGLFPHMSIAANIGITPRLLGTPKSEIAARVDELLDLVQLDRAQYRDRLPHELSGGQRQRVGVARALAAKPRIVLMDEPFGALDPVTRDALGNDYRALHIGLGLTTIMITHDMTEALLLADRVAVMHEGRLVALGTAKELSRIDSPYVGELLGTPRRQAERLQAALSQNIAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ61</accession>
    <name>Q3SQ61_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Heme utilization protein HuvX</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2326</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315545.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2326"/>
    <dbReference type="EnsemblBacteria" id="ABA05580">
        <property type="protein sequence ID" value="ABA05580"/>
        <property type="gene ID" value="Nwi_2326"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2326"/>
    <dbReference type="eggNOG" id="COG3721">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106714_0_0_5"/>
    <dbReference type="OMA" id="HSFGSIF"/>
    <dbReference type="OrthoDB" id="1938973at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd16829">
        <property type="entry name" value="ChuX_HutX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010413">
        <property type="entry name" value="HutX-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06228">
        <property type="entry name" value="ChuX_HutX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF030840">
        <property type="entry name" value="DUF1008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04108">
        <property type="entry name" value="HutX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="176" mass="19287" checksum="5EFA88ABAE27C430" modified="2005-10-11" version="1">MTGALASDLRTYLAENPGAVIEDVARDRHVPPRTVIEALPSGMVKLCQGGVFTDAMQDITQWGEVTLIVHTADAIFEFTGPIPNGEIGRGYFNLMQPKGLHGHLRHERCAAIAFVERPFMGKSSAFVAFLNVDGGVMFKVFVGRDETRALRQDQLERFRALAERLGAVPASEEICS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQE4</accession>
    <name>Q3SQE4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nitrous oxidase accessory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2243</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315465.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2243"/>
    <dbReference type="EnsemblBacteria" id="ABA05497">
        <property type="protein sequence ID" value="ABA05497"/>
        <property type="gene ID" value="Nwi_2243"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2243"/>
    <dbReference type="eggNOG" id="COG5434">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032301_0_0_5"/>
    <dbReference type="OMA" id="WGPYLRN"/>
    <dbReference type="OrthoDB" id="507585at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039448">
        <property type="entry name" value="Beta_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022388">
        <property type="entry name" value="CHP03808"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022444">
        <property type="entry name" value="Cofactor-bd_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13229">
        <property type="entry name" value="Beta_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03807">
        <property type="entry name" value="RR_fam_repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03808">
        <property type="entry name" value="RR_plus_rpt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta_helix" evidence="1">
        <location>
            <begin position="141"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="455" mass="46312" checksum="5118A92554BB2A7D" modified="2005-10-11" version="1">MDIDRRHILGASAAGIIGAQAMPANAVRAAPLTSTLGRDAAHYGVRPNATGDQTRALQRAINEAARANAPLALSPGVYRTGPLLLAKGSQLVGVRGATTLAFTGGASLLAAEGADHIGLSDLTLDGGGVPLPDRRGLVHCLGGRDVRIMDCAITGSGGNGIWLENAAGEISGNLIEKIETTGIVSFDALGLTIARNTIRQTRDNGIEILRTAIGDDGSIVTGNRIENIKAGQGGSGQHGNAINAFRAGNVIVSGNRISDCDYSAVRGNSASNIQITGNSISNVREVALYSEFAFEGAVIAGNTVDGAAVGVSVSNFNKGGRLAVVQGNIIRNLLPKRPIGTAPDDDAGIGIYIEADTSVTGNLIENAPSFGIIAGWGKYLRDVAITGNVIRKTFIGIGVSVVDGAGTALVNTNVISDAPRGAVVGLDHARPVTPDLSAAGAAKFTQIVIGANAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQE7</accession>
    <name>Q3SQE7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methylmalonyl-CoA mutase metallochaperone MeaB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIMIBI class G3E GTPase family. ArgK/MeaB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315462.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2240"/>
    <dbReference type="EnsemblBacteria" id="ABA05494">
        <property type="protein sequence ID" value="ABA05494"/>
        <property type="gene ID" value="Nwi_2240"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2240"/>
    <dbReference type="eggNOG" id="COG1703">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043725_2_2_5"/>
    <dbReference type="OMA" id="WMWERID"/>
    <dbReference type="OrthoDB" id="1134430at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005129">
        <property type="entry name" value="GTPase_ArgK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23408">
        <property type="entry name" value="PTHR23408"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00750">
        <property type="entry name" value="lao"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="326" mass="34740" checksum="5067167FCA9964E9" modified="2005-10-11" version="1">MNRPLAIPGIAADIRAGNRAALARAITLIESRRADHEATARELVQELLPATGNAIRVGITGSPGVGKSTTIDALGMDLIARGHRIAVLAVDPSSVRSGGSILGDKTRMARLAAAEAAYIRPSPAAGTLGGVAARTREAMLLCEAAGFDVILVETVGTGQSETAVYDMTDFFLALMLPGAGDELQGIKKGLVELADMIAVNKADGDNLSRANTAAAAYRSAMKILVPRSQHWSPPVLTYSGLTGSGIPELWRKIVEHRAAMNRSGEFAQRRSRQQVKWMWSMFEDRIKARLRNDPVIREKVKATEAAVSEGRVTPALGASQIAELLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQE8</accession>
    <name>Q3SQE8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF3298 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2239</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2239"/>
    <dbReference type="EnsemblBacteria" id="ABA05493">
        <property type="protein sequence ID" value="ABA05493"/>
        <property type="gene ID" value="Nwi_2239"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2239"/>
    <dbReference type="eggNOG" id="ENOG5031DQ0">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1056323_0_0_5"/>
    <dbReference type="OMA" id="SGLTFHY"/>
    <dbReference type="OrthoDB" id="1624082at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021729">
        <property type="entry name" value="DUF3298"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037126">
        <property type="entry name" value="PdaC/RsiV-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11738">
        <property type="entry name" value="DUF3298"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229132" description="DUF3298 domain-containing protein" evidence="1">
        <location>
            <begin position="33"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3298" evidence="2">
        <location>
            <begin position="187"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="270" mass="29499" checksum="09377DAC832CB53C" modified="2005-10-11" version="1">MITRLSSIVISLQKFSCLAAALVLAGIAPTIAAQPKPDVAVKTKYIDASVTLDKQIRANAPLAENLLAEGKHWVEKNRADAAKEFKTSPELFRDGRAWTFERSYAQTSNVGDRYVSIVRTDYVYTGRAHPNTLIDTILWDEETRKQISIRPFFKETADTGPTLKTLRNAAIAAVKAEKKERGIEDSGDIDWYQGILPALLKVGAISLAPSTVAGKSAGLAFHYSPYAVGPYAEGSYTVFVPWETFKAHLSPQGLAIFGGDRPPDTKPDAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQP5</accession>
    <name>Q3SQP5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phage integrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2142</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315365.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2142"/>
    <dbReference type="EnsemblBacteria" id="ABA05396">
        <property type="protein sequence ID" value="ABA05396"/>
        <property type="gene ID" value="Nwi_2142"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2142"/>
    <dbReference type="eggNOG" id="COG0582">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027562_0_4_5"/>
    <dbReference type="OMA" id="GVRAIYN"/>
    <dbReference type="OrthoDB" id="785253at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038488">
        <property type="entry name" value="Integrase_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025166">
        <property type="entry name" value="Integrase_DNA_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13356">
        <property type="entry name" value="Arm-DNA-bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyr recombinase" evidence="3">
        <location>
            <begin position="199"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="418" mass="47599" checksum="B3F47628B5CE53BC" modified="2005-10-11" version="1">MLTDVALKNLKPKEKPYKVTDRDGMYVLVSTTGTVSFRLDYRLNGRRETVVLGRYDKAGISLARARELCIDAKRMIREGRSPAFEKQRAKRRLLEAKSFGEFGEKWLTMAPMADSTRSMRRAIFERELLPTWRKRLLTEITPDDLRAHCGAIVDRGAPATALHVRDIVKQIYGFAILHGEKVANPADEVGPSAIAHFTPRDRSLSPTEIRIMLKQLENVATLPTIRLGLKLILLTMVRKSELQDAVWDEIDFENGVWTIPKERMKRSKAHNVYLSRQALDIFIALKTCAGNSRYVLPSRYDADAPMSRATFNRVTYLVVEQAKKEGLPLEPFTVHDLRRTGSTLLNELGFNSDWIEKCLAHEEGRSSRGVYNKAEYEVQRRHMLQEWSNIVDAWVAGEKYVPVLIPPSMPLLAPDPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQP7</accession>
    <name>Q3SQP7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2140</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2140"/>
    <dbReference type="EnsemblBacteria" id="ABA05394">
        <property type="protein sequence ID" value="ABA05394"/>
        <property type="gene ID" value="Nwi_2140"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2140"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2634521_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="77" mass="8216" checksum="35E23410DBC44D27" modified="2005-10-11" version="1">MNQLAAVKSGIGLALLPCYLGEVGVVRALREPIADLEGELWIGTHADLKGTARVRAFFDIVGAGLAHESQWLEGRTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQS7</accession>
    <name>Q3SQS7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Filamentation induced by cAMP protein Fic</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2109</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315337.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05364">
        <property type="protein sequence ID" value="ABA05364"/>
        <property type="gene ID" value="Nwi_2109"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2109"/>
    <dbReference type="eggNOG" id="COG3177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047250_1_1_5"/>
    <dbReference type="OMA" id="PRWLDTF"/>
    <dbReference type="OrthoDB" id="1232677at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025758">
        <property type="entry name" value="Fic/DOC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003812">
        <property type="entry name" value="Fido"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036597">
        <property type="entry name" value="Fido-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040198">
        <property type="entry name" value="Fido_containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026287">
        <property type="entry name" value="SoFic-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13504">
        <property type="entry name" value="PTHR13504"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02661">
        <property type="entry name" value="Fic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13784">
        <property type="entry name" value="Fic_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038925">
        <property type="entry name" value="AMP-prot_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140931">
        <property type="entry name" value="SSF140931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51459">
        <property type="entry name" value="FIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fido" evidence="4">
        <location>
            <begin position="132"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="217"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="221"/>
            <end position="228"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="222"/>
            <end position="228"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="259"/>
            <end position="260"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR038925-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR640198-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR640198-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="391" mass="43975" checksum="BEB4C07D7511EF73" modified="2005-10-11" version="1">MPKRTTGRYESVTAGGEKVDAFVPFDLPPARPSLAIDAKFERRLGQAEQALVRLDLASEMVPSLDWFIYAFVRKEAVITSQIEGTQASLVDLLAFEAEEQPSPNADVEEVTNYLDALTYARTQLGSKRGLPISLRLLNEAHKRLMRGGRGASKQPGEIRRSQNWIGGSRPGNAVYVPPPSDKLPKLLGAFEKYIHAEDRLPKLVRTALLHAQFESIHPYLDGNGRIGRLMIALLLEHWGLLKQPLLYLSLFFKRHREEYYRRLNLVRLEGDWEGWTDFFLDGVATIADEAVASARELFNLVSTDRTRLLGVESASVSAFRLFELLPRHPIVSVASAMRLLGTSKPTATRAIEVLTEIGILVETTGKKRDRSFAYRAYMERLRTGTELDLSR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-07-24" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR08</accession>
    <name>PANB_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-methyl-2-oxobutanoate hydroxymethyltransferase</fullName>
            <ecNumber evidence="1">2.1.2.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Ketopantoate hydroxymethyltransferase</fullName>
            <shortName evidence="1">KPHMT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">panB</name>
        <name type="ordered locus">Nwi_2024</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is transferred onto alpha-ketoisovalerate to form ketopantoate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = (6S)-5,6,7,8-tetrahydrofolate + 2-dehydropantoate</text>
            <dbReference type="Rhea" id="RHEA:11824"/>
            <dbReference type="ChEBI" id="CHEBI:11561"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodecamer; pentamer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PanB family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.11" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315269.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SR08"/>
    <dbReference type="SMR" id="Q3SR08"/>
    <dbReference type="STRING" id="323098.Nwi_2024"/>
    <dbReference type="EnsemblBacteria" id="ABA05283">
        <property type="protein sequence ID" value="ABA05283"/>
        <property type="gene ID" value="Nwi_2024"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2024"/>
    <dbReference type="eggNOG" id="COG0413">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036645_1_0_5"/>
    <dbReference type="OMA" id="VLVWTDM"/>
    <dbReference type="OrthoDB" id="916845at2"/>
    <dbReference type="UniPathway" id="UPA00028">
        <property type="reaction ID" value="UER00003"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003864">
        <property type="term" value="F:3-methyl-2-oxobutanoate hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06557">
        <property type="entry name" value="KPHMT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00156">
        <property type="entry name" value="PanB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003700">
        <property type="entry name" value="Pantoate_hydroxy_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20881">
        <property type="entry name" value="PTHR20881"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02548">
        <property type="entry name" value="Pantoate_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000388">
        <property type="entry name" value="Pantoate_hydroxy_MeTrfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00222">
        <property type="entry name" value="panB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0566">Pantothenate biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000297308" description="3-methyl-2-oxobutanoate hydroxymethyltransferase">
        <location>
            <begin position="1"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="187"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="49"/>
            <end position="50"/>
        </location>
        <ligand>
            <name>3-methyl-2-oxobutanoate</name>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>3-methyl-2-oxobutanoate</name>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>3-methyl-2-oxobutanoate</name>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00156"/>
        </source>
    </evidence>
    <sequence length="274" mass="29462" checksum="665D5959597422BE" modified="2005-10-11" version="1">MSVQSAIRRRTAPDIRARKNGDPIVMLTSYHAHTAALVDRYCDVILVGDSLGNVMHGFETTVPVTLDMMILQGRAVMRGSQQALVVVDMPFGSYEASKEQAFHSAARLLKETLCGAVKLEGGVKMAETIAFLSARGIPVMGHVGLTPQSINTLGSFRAQGREEGTWQPIEDDARAVAEAGAFSMVIEAVAEPLARRITETVAIPTIGIGASPACDGQVLVLEDMLGLSPRAPKFVRRYGELGPMIEAAIEGYARDVRSRAFPGPEHVYAMKPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="128" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR18</accession>
    <name>Q3SR18_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">Alanine racemase</fullName>
            <ecNumber evidence="4 6">5.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_2014</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>L-alanine = D-alanine</text>
            <dbReference type="Rhea" id="RHEA:20249"/>
            <dbReference type="ChEBI" id="CHEBI:57416"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="5.1.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 6 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the alanine racemase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.1.1" evidence="4 6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315259.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2014"/>
    <dbReference type="EnsemblBacteria" id="ABA05273">
        <property type="protein sequence ID" value="ABA05273"/>
        <property type="gene ID" value="Nwi_2014"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2014"/>
    <dbReference type="eggNOG" id="COG0787">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028393_1_1_5"/>
    <dbReference type="OMA" id="WEILCGF"/>
    <dbReference type="OrthoDB" id="859043at2"/>
    <dbReference type="UniPathway" id="UPA00042">
        <property type="reaction ID" value="UER00497"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008784">
        <property type="term" value="F:alanine racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030632">
        <property type="term" value="P:D-alanine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01201">
        <property type="entry name" value="Ala_racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000821">
        <property type="entry name" value="Ala_racemase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011079">
        <property type="entry name" value="Ala_racemase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001608">
        <property type="entry name" value="Ala_racemase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020622">
        <property type="entry name" value="Ala_racemase_pyridoxalP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00842">
        <property type="entry name" value="Ala_racemase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01168">
        <property type="entry name" value="Ala_racemase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00992">
        <property type="entry name" value="ALARACEMASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01005">
        <property type="entry name" value="Ala_racemase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="SSF50621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="SSF51419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00492">
        <property type="entry name" value="alr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00395">
        <property type="entry name" value="ALANINE_RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ala_racemase_C" evidence="9">
        <location>
            <begin position="255"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; specific for D-alanine" evidence="6">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; specific for L-alanine" evidence="6">
        <location>
            <position position="276"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 8">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 8">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6 7">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600821-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR600821-52"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA05273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="395" mass="41331" checksum="383B8F6EEBD9BA6D" modified="2005-10-11" version="1">MNLVPDSKVSGEPADAGAAIAAAAAIGTLTVDLDAIVANWRKLEKTAVPAECAGVIKADAYGCGARPVARALSAAGCRTFFVATLDEARVVRAAASTADIYVLDGFFQNCGDAFATINCRPVIGDLNELAEWDVFCRRTGWAGGAAIHIDTGMNRLGLTLDEAQGILPRINAGDHGVTLVMSHLACAESLHHPLNASQVAAFREIARQFSGVPASLANSSGIFLGAQYQFDLVRPGAALYGVNPTPEAANPMQPVIELKARIIQTRSIDRGESVGYGATWTARRPTRLAVVSAGYADGYFRAGGSNDGTRGAEVVVAGTRCPVAGRISMDLLAVDITDLPAHAGVRRGHLATLIGEGITVDELAHHFGTIGYEVLTSLGGRYHRIYKGGPAEPRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR69</accession>
    <name>Q3SR69_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1962</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315213.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1962"/>
    <dbReference type="EnsemblBacteria" id="ABA05222">
        <property type="protein sequence ID" value="ABA05222"/>
        <property type="gene ID" value="Nwi_1962"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1962"/>
    <dbReference type="eggNOG" id="ENOG5033CNU">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_177872_0_0_5"/>
    <dbReference type="OMA" id="QPEMYLR"/>
    <dbReference type="OrthoDB" id="1814992at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="98" mass="10491" checksum="B4BF9C80A0037C21" modified="2005-10-11" version="1">MSITKKLAVLREQHYGLDKLPETIRVPALGERRDETVKPVGAASIDDLAFALIGLNERASALYREIDAVRTLHDEARKAGALGADIAIDALIAAKGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR89</accession>
    <name>Q3SR89_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative bacteriophage-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1942</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315195.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1942"/>
    <dbReference type="EnsemblBacteria" id="ABA05202">
        <property type="protein sequence ID" value="ABA05202"/>
        <property type="gene ID" value="Nwi_1942"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1942"/>
    <dbReference type="eggNOG" id="COG1961">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132664_0_0_5"/>
    <dbReference type="OMA" id="RWRRMLE"/>
    <dbReference type="OrthoDB" id="1362093at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="136" mass="14930" checksum="7F485D96038E4AA5" modified="2005-10-11" version="1">MDSSAAKVQDAITVHVPLTFAKRGGRKQVVLPDGTSSWEPPRARIDNTAVKAIARAFRWRNMLETGVHATIADIAMAEKINDSYASRVLRLTLLAPDIIEAILNGRQGPEVTLAALMKPFPVEWERQRSLFAAPIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRA7</accession>
    <name>Q3SRA7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NYN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1924</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315180.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1924"/>
    <dbReference type="EnsemblBacteria" id="ABA05184">
        <property type="protein sequence ID" value="ABA05184"/>
        <property type="gene ID" value="Nwi_1924"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1924"/>
    <dbReference type="eggNOG" id="COG1432">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092340_0_0_5"/>
    <dbReference type="OMA" id="CSHLRHE"/>
    <dbReference type="OrthoDB" id="1891199at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021139">
        <property type="entry name" value="NYN_MARF1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01936">
        <property type="entry name" value="NYN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NYN" evidence="2">
        <location>
            <begin position="7"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="205" mass="23148" checksum="33FB17B9A9DBF669" modified="2005-10-11" version="1">MPSASNKIALFIDGANLYATAKTLGFDIDYKRLLKEFQSRGTLVRAFYYTAIIEDQEYSSIRPLIDWLDYNGYTVVTKATKEFIDASGRRKVKGNMDIELAVDAMELAEHVDQIVLFSGDGDFRSLVEAVQRRGVRVTVVSTIASQPPMIADELRRQADVFTDLVELQPKLGRDPSERQSRHHAPQFLQRATAVAPRSDDDDFED</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRE0</accession>
    <name>Q3SRE0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1891</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315147.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05151">
        <property type="protein sequence ID" value="ABA05151"/>
        <property type="gene ID" value="Nwi_1891"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1891"/>
    <dbReference type="eggNOG" id="ENOG5032ZWG">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_173298_1_0_5"/>
    <dbReference type="OMA" id="FAYGVIQ"/>
    <dbReference type="OrthoDB" id="2010287at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021529">
        <property type="entry name" value="DUF2798"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11391">
        <property type="entry name" value="DUF2798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="79" mass="8698" checksum="A5D749C9327047D3" modified="2005-10-11" version="1">MLGIPRRYCHFVFGVIQAGLTSFIAAAIASISLWGSGRFLYNWLISWVVSWAIMLPAVLFAAPVIQFIAVHLTRDDCAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRF8</accession>
    <name>Q3SRF8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Biotin--[biotin carboxyl-carrier protein] ligase</fullName>
            <ecNumber evidence="3">6.3.4.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_1873</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + biotin + L-lysyl-[protein] = AMP + diphosphate + H(+) + N(6)-biotinyl-L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:11756"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10505"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
            <dbReference type="ChEBI" id="CHEBI:83144"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.15"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="6.3.4.15" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315129.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1873"/>
    <dbReference type="EnsemblBacteria" id="ABA05133">
        <property type="protein sequence ID" value="ABA05133"/>
        <property type="gene ID" value="Nwi_1873"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1873"/>
    <dbReference type="eggNOG" id="COG0340">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051096_3_0_5"/>
    <dbReference type="OMA" id="AVWKHIE"/>
    <dbReference type="OrthoDB" id="1842956at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004077">
        <property type="term" value="F:biotin-[acetyl-CoA-carboxylase] ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd16442">
        <property type="entry name" value="BPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004408">
        <property type="entry name" value="Biotin_CoA_COase_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003142">
        <property type="entry name" value="BPL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008988">
        <property type="entry name" value="Transcriptional_repressor_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02237">
        <property type="entry name" value="BPL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03099">
        <property type="entry name" value="BPL_LplA_LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50037">
        <property type="entry name" value="SSF50037"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00121">
        <property type="entry name" value="birA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0092">Biotin</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="5">
        <location>
            <begin position="41"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="BPL_C" evidence="4">
        <location>
            <begin position="217"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="270" mass="28032" checksum="680F6CEC1CF32544" modified="2005-10-11" version="1">MAFSLGPRAASAGYRLAVFDRIGSTNAEALARARAGETGPIWLVAAEQTAGHGRRQRSWISPRGNLACSVLEVIDTSPAVAATLSFAAGLALEAALRTVSLEARMRSPGSENVTFHLKWPNDVLAGGGKLAGVLLEAETVAPDRLAVVVGTGANVAAAPEGMPYPAASLRDIGIDIGAEDLFGALTDAWAEFRIVWDKGRGFGEIRRLWLERAAGVGQHVSIRSDSSTISGLFDTIDKDGCLIVVTSDGRRVAVSAGDVYFGAAASVGAT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRI5</accession>
    <name>LPXB_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lipid-A-disaccharide synthase</fullName>
            <ecNumber evidence="1">2.4.1.182</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lpxB</name>
        <name type="ordered locus">Nwi_1846</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a lipid X + a UDP-2-N,3-O-bis[(3R)-3-hydroxyacyl]-alpha-D-glucosamine = a lipid A disaccharide + H(+) + UDP</text>
            <dbReference type="Rhea" id="RHEA:67828"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:137748"/>
            <dbReference type="ChEBI" id="CHEBI:176338"/>
            <dbReference type="ChEBI" id="CHEBI:176343"/>
            <dbReference type="EC" id="2.4.1.182"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Bacterial outer membrane biogenesis; LPS lipid A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LpxB family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.182" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315102.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRI5"/>
    <dbReference type="SMR" id="Q3SRI5"/>
    <dbReference type="STRING" id="323098.Nwi_1846"/>
    <dbReference type="CAZy" id="GT19">
        <property type="family name" value="Glycosyltransferase Family 19"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05106">
        <property type="protein sequence ID" value="ABA05106"/>
        <property type="gene ID" value="Nwi_1846"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1846"/>
    <dbReference type="eggNOG" id="COG0763">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036577_3_0_5"/>
    <dbReference type="OMA" id="PTVWAWR"/>
    <dbReference type="OrthoDB" id="1258510at2"/>
    <dbReference type="UniPathway" id="UPA00973"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008915">
        <property type="term" value="F:lipid-A-disaccharide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009245">
        <property type="term" value="P:lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00392">
        <property type="entry name" value="LpxB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003835">
        <property type="entry name" value="Glyco_trans_19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30372">
        <property type="entry name" value="PTHR30372"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02684">
        <property type="entry name" value="LpxB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00215">
        <property type="entry name" value="lpxB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0441">Lipid A biosynthesis</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000255202" description="Lipid-A-disaccharide synthase">
        <location>
            <begin position="1"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00392"/>
        </source>
    </evidence>
    <sequence length="396" mass="43640" checksum="BBDE4D4CFCE7CAD7" modified="2005-10-11" version="1">MPDEASANPSRKIFLIATEESGDRLGSSLMKALRRRLGCSVRFEGVGGQTMAREGLVSLFPIEELSIMGFTAVVKQLPMIVRRIRGTADAVIAAAPDVLVIIDSPDFTHRVARRVRARCRGLPIVDYVSPSVWAWRSGRARAMRSYVDHVLALLPFEPEAYRRLGGPPCTYVGHPLLEQIGVLRPDAQERQRRDADPPTLLVLPGSRRSEIRHHLSVFGETIGMLQQSIPEIEVVLPTTPHLVDEITPAVATWPRRPRVVIGEDDKRAAFRVARAALAKSGTVTLELALAGVPMVAAYKAGSVEAWIARRVIRVSSVILANLVIRENVIPEFLQEDCVPGKLAPALQEILTDSPMRRRQLKAFDGLNTIMATGQRSPSELAADIVIEAMRESRARM</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRR1</accession>
    <name>Q3SRR1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein translocase subunit SecD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">secD</name>
        <name evidence="10" type="ordered locus">Nwi_1769</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Forms a complex with SecF. Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF-YajC and YidC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell inner membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SecD/SecF family. SecD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315026.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1769"/>
    <dbReference type="EnsemblBacteria" id="ABA05030">
        <property type="protein sequence ID" value="ABA05030"/>
        <property type="gene ID" value="Nwi_1769"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1769"/>
    <dbReference type="eggNOG" id="COG0342">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007894_4_3_5"/>
    <dbReference type="OMA" id="SAHLQMM"/>
    <dbReference type="OrthoDB" id="121331at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015450">
        <property type="term" value="F:protein-transporting ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043952">
        <property type="term" value="P:protein transport by the Sec complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01463_B">
        <property type="entry name" value="SecD_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001036">
        <property type="entry name" value="Acrflvin-R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005791">
        <property type="entry name" value="SecD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022813">
        <property type="entry name" value="SecD/SecF_arch_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022645">
        <property type="entry name" value="SecD/SecF_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022646">
        <property type="entry name" value="SecD/SecF_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30081">
        <property type="entry name" value="PTHR30081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07549">
        <property type="entry name" value="Sec_GG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02355">
        <property type="entry name" value="SecD_SecF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00702">
        <property type="entry name" value="ACRIFLAVINRP"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00916">
        <property type="entry name" value="2A0604s01"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01129">
        <property type="entry name" value="secD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="373"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="395"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="421"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="466"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="494"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA05030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="532" mass="57644" checksum="6F28F520E5F79864" modified="2005-10-11" version="1">MLYFTRWKALTIIVTALAICLCAVPNFFPEAQVKTWPKWAQRHLVLGLDLQGGSYLLLEVDSNYVKKEKLDQVRDDARKTLRDARIAYTGLAAKNGAVEVRISKEEEFQTALAKLRELSQPLGGLLGSSGQRSLEVSSADGVIRMTVPQAAVTDRIRQSVEQSIQIIEKRVNELGTVEPLIQRQGADRILVQVPGLQDPTRLKEILGKTAKMDFRMVDSSIPPDQAAQGRVPGDSEVLMSSQAPKTPYVVRKQVLVSGGDLTDAQPGFDSRTSEPIVTFRFNSNGARKFAQATTENVGQPFAIILDNEVISAPVIREPITGGSGQISGNFTVQEANDLAILLRAGALPAPLTIIEERTVGPGLGQDSIEQGELAAYVGSIMVILFMLLTYRFFGVIANIAVAINVAMIFGVLSLLNATLTLPGIAGIVLTVGIAVDSNVLIYERIREELRAGRTAISAIDAGFKRALATILDSNITTFIAAAVLFYIGTGPVRGFAVTFGIGIITTVFTAFEVTRLIVATWVRWKRPQTVPI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRS9</accession>
    <name>AROQ_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-dehydroquinate dehydratase</fullName>
            <shortName evidence="1">3-dehydroquinase</shortName>
            <ecNumber evidence="1">4.2.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Type II DHQase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aroQ</name>
        <name type="ordered locus">Nwi_1751</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes a trans-dehydration via an enolate intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3-dehydroquinate = 3-dehydroshikimate + H2O</text>
            <dbReference type="Rhea" id="RHEA:21096"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="EC" id="4.2.1.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homododecamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-II 3-dehydroquinase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.10" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315008.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRS9"/>
    <dbReference type="SMR" id="Q3SRS9"/>
    <dbReference type="STRING" id="323098.Nwi_1751"/>
    <dbReference type="EnsemblBacteria" id="ABA05012">
        <property type="protein sequence ID" value="ABA05012"/>
        <property type="gene ID" value="Nwi_1751"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1751"/>
    <dbReference type="eggNOG" id="COG0757">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090968_2_0_5"/>
    <dbReference type="OMA" id="CAGIVIN"/>
    <dbReference type="OrthoDB" id="1872106at2"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00086"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003855">
        <property type="term" value="F:3-dehydroquinate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00466">
        <property type="entry name" value="DHQase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.9100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00169">
        <property type="entry name" value="AroQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001874">
        <property type="entry name" value="DHquinase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018509">
        <property type="entry name" value="DHquinase_II_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036441">
        <property type="entry name" value="DHquinase_II_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21272">
        <property type="entry name" value="PTHR21272"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01220">
        <property type="entry name" value="DHquinase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001399">
        <property type="entry name" value="DHquinase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52304">
        <property type="entry name" value="SSF52304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01088">
        <property type="entry name" value="aroQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01029">
        <property type="entry name" value="DEHYDROQUINASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000023492" description="3-dehydroquinate dehydratase">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="24"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="103"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00169"/>
        </source>
    </evidence>
    <sequence length="156" mass="16620" checksum="C7550F9DCE14B2FC" modified="2005-10-11" version="1">MAKTIYVLNGPNLNMLGTREPDTYGHANLADVEQLCAETAKNFGLAADCRQSNREGELIDFIHEAHAKNAAGIVINAGGYSHTSIALHDALVAVKIPTVEVHISNIHAREDFRHHSFTAKAAFASLSGFGIDGYRLAINGLAAKIGAVDPSNKVKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="126" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRU1</accession>
    <name>Q3SRU1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">S-(hydroxymethyl)glutathione dehydrogenase</fullName>
            <ecNumber evidence="5 7">1.1.1.284</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_1739</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>NAD(+) + S-(hydroxymethyl)glutathione = H(+) + NADH + S-formylglutathione</text>
            <dbReference type="Rhea" id="RHEA:19985"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57688"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58758"/>
            <dbReference type="EC" id="1.1.1.284"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>NADP(+) + S-(hydroxymethyl)glutathione = H(+) + NADPH + S-formylglutathione</text>
            <dbReference type="Rhea" id="RHEA:19981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57688"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58758"/>
            <dbReference type="EC" id="1.1.1.284"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3 7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4 7">Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.284" evidence="5 7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314996.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1739"/>
    <dbReference type="EnsemblBacteria" id="ABA05000">
        <property type="protein sequence ID" value="ABA05000"/>
        <property type="gene ID" value="Nwi_1739"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1739"/>
    <dbReference type="eggNOG" id="COG1062">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_14_0_5"/>
    <dbReference type="OMA" id="IHHYMGT"/>
    <dbReference type="OrthoDB" id="1859760at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106322">
        <property type="term" value="F:S-(hydroxymethyl)glutathione dehydrogenase NAD activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106321">
        <property type="term" value="F:S-(hydroxymethyl)glutathione dehydrogenase NADP activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006069">
        <property type="term" value="P:ethanol oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08300">
        <property type="entry name" value="alcohol_DH_class_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014183">
        <property type="entry name" value="ADH_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02818">
        <property type="entry name" value="adh_III_F_hyde"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6 7 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="ADH_N" evidence="9">
        <location>
            <begin position="28"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="ADH_zinc_N" evidence="8">
        <location>
            <begin position="197"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA05000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="375" mass="39945" checksum="08BF35148DB0A528" modified="2005-10-11" version="1">MHVRAAVALAAGKPLEVTTVELDGPRDGEVMVEIKAAGVCHTDEFTLSGADPEGLFPAILGHEGAGVVVDVGKGVTSVKKGDHVIPLYTPECRQCPSCLSRKTNLCTAIRATQGQGLMPDGTSRFSFDGKPLHHYMGTSTFANYTVLPEIAVAKIREDAPFDKVCYIGCGVTTGIGAVINTAKVEPGAKAIVFGLGGIGLNVLQGLRLAGADMIIGVDINDDRKAWGERFGMTHFVNPTDVGKELVPYLVDMTKSGADQIGGADYTFDCTGNVKVMRQALESCHRGWGQSIVIGVAPAGAEIATRPFQLVTGRVWKGTAFGGARGRTDVPKIVDWYMQGKIQIDPMITHVMPLDDINRAFDLMRKGESIRGVVTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS60</accession>
    <name>Q3SS60_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1620</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314885.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1620"/>
    <dbReference type="EnsemblBacteria" id="ABA04881">
        <property type="protein sequence ID" value="ABA04881"/>
        <property type="gene ID" value="Nwi_1620"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1620"/>
    <dbReference type="eggNOG" id="ENOG5031NKU">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126531_1_0_5"/>
    <dbReference type="OMA" id="WVYLDLH"/>
    <dbReference type="OrthoDB" id="1910502at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021508">
        <property type="entry name" value="Gp17-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11367">
        <property type="entry name" value="DUF3168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="144" mass="15776" checksum="6BF93DB11A944808" modified="2005-10-11" version="1">MSSPALALQKAIRLRLINTAAVTALVPAANILDRNERPAPSPSIILGEDQEVDEDFTLKRDYIRVYSTLHIWKTEPSTAGVKAIAGAVRKAIGRVRRLDIGDPDFVCADCRVDGVRYLRDPGGEMSHAVLTINSLVQARWSVVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS77</accession>
    <name>Q3SS77_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1603</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1603"/>
    <dbReference type="EnsemblBacteria" id="ABA04864">
        <property type="protein sequence ID" value="ABA04864"/>
        <property type="gene ID" value="Nwi_1603"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1603"/>
    <dbReference type="HOGENOM" id="CLU_2789730_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="68" mass="7707" checksum="4CB367621B6DCED4" modified="2005-10-11" version="1">MAPTAAFAGKGWHHHGHHHHHGHGHHHHGWRHYAWGPGHFIGGGGGCYVRRLVPTSFGPRWRVVNRCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSB6</accession>
    <name>Q3SSB6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phage Gp37Gp68</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1564</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1564"/>
    <dbReference type="EnsemblBacteria" id="ABA04825">
        <property type="protein sequence ID" value="ABA04825"/>
        <property type="gene ID" value="Nwi_1564"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1564"/>
    <dbReference type="eggNOG" id="COG4422">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054184_0_0_5"/>
    <dbReference type="OMA" id="KVSPGCK"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011101">
        <property type="entry name" value="DUF5131"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07505">
        <property type="entry name" value="DUF5131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="397"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="419" mass="46478" checksum="EC9CFEC2B1281093" modified="2005-10-11" version="1">MAEHSTIEWTDATWQPITGCSVVSPGCTNCYAMRLAGTRLKHHPSRANLTTMTKGGPVWNGAVRLNPEWLTQPLGWKRPRRIFVCAHGDLFHESVPDEWIDQIFAVMALTPQHTYQVLTKRSARMRDYICTRAGDWQVVLPDACAPGTLPITKNDVARALGRLPKFSYDPPTPTWPLANVWLGVSTERQKEADERIPDLLATPAAVHFISAEPLLGPIDLTAIKATHVPGTPDDSDMGWTFNSLATGDYYSFKDDRGYNEGGDGPHREHALDWVIVGGESGPDARPMHPDWARSIRDQCAAAGVPFFFKQWGSWYPIVDRDKEDPDWRADYGRASRSPEKFRILNLAGGCGFHGDSVHLMQRYSKSATGRLLDGVTYDAMPKAVAEQLIARQRPSIQNAGDALDEQTRGAPISVRGAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSC7</accession>
    <name>Q3SSC7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1553</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314820.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1553"/>
    <dbReference type="EnsemblBacteria" id="ABA04814">
        <property type="protein sequence ID" value="ABA04814"/>
        <property type="gene ID" value="Nwi_1553"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1553"/>
    <dbReference type="eggNOG" id="ENOG50331RJ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1494749_0_0_5"/>
    <dbReference type="OrthoDB" id="2025215at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="180" mass="20657" checksum="9EFCCF86D5A3AEA9" modified="2005-10-11" version="1">MKQSKWMSVLESIINIVVGFGISMTAQAVFLPMLGVPIPWHANFVFALIMTAISIVRSFTLRRLFEALHIRRPLSPFMQAVIAERYRQQDVEGWDAAHDDEHARGDLARAGAAYLKQADCHLIEGADVGRDAKFYLPLFWPWEIEWWKPTGFRRDLVKGCALGIAEGEKFDRNRSRKVQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSD7</accession>
    <name>Q3SSD7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ATP-dependent Clp protease proteolytic subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1543</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the peptidase S14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314810.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1543"/>
    <dbReference type="EnsemblBacteria" id="ABA04804">
        <property type="protein sequence ID" value="ABA04804"/>
        <property type="gene ID" value="Nwi_1543"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1543"/>
    <dbReference type="eggNOG" id="COG0740">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052762_3_1_5"/>
    <dbReference type="OMA" id="RGAFLMI"/>
    <dbReference type="OrthoDB" id="1728970at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001907">
        <property type="entry name" value="ClpP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023562">
        <property type="entry name" value="ClpP/TepA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10381">
        <property type="entry name" value="PTHR10381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00574">
        <property type="entry name" value="CLP_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00127">
        <property type="entry name" value="CLPPROTEASEP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="281" mass="30309" checksum="4F4B404A9B1914DE" modified="2005-10-11" version="1">MSLRKLPEARTFQRPQNFQWDAPSDVLARWSETPRAAASDDDATISMYEVIGEDWWSGGGVTAKRISAALRSIGDRDVTVKINSPGGDMFEGIAIYNLLRGHPARVTVEVLGWAASAASIIAMAGDEIRMGLGTFMMVHNAWGVVIGNRHDMRESAELFDGFDSAIVDIYEARTGLKRSAIEKLMDAETFMGPSEAVKNGFADLVDDAIGADQSEAENSVAGAVNARRRMDAALAKQGVPRVERRRMFAELAGGTHDAAPTATHDAGLVAAARQFINAIRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSE6</accession>
    <name>Q3SSE6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidoglycan glycosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.129</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">Nwi_1534</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = [GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23708"/>
            <dbReference type="Rhea" id="RHEA-COMP:9602"/>
            <dbReference type="Rhea" id="RHEA-COMP:9603"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:60033"/>
            <dbReference type="ChEBI" id="CHEBI:78435"/>
            <dbReference type="EC" id="2.4.1.129"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the transpeptidase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the glycosyltransferase 51 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.129" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314801.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1534"/>
    <dbReference type="CAZy" id="GT51">
        <property type="family name" value="Glycosyltransferase Family 51"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04795">
        <property type="protein sequence ID" value="ABA04795"/>
        <property type="gene ID" value="Nwi_1534"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1534"/>
    <dbReference type="eggNOG" id="COG4953">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006354_7_3_5"/>
    <dbReference type="OMA" id="APENFDM"/>
    <dbReference type="OrthoDB" id="652304at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008658">
        <property type="term" value="F:penicillin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008955">
        <property type="term" value="F:peptidoglycan glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3810.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001264">
        <property type="entry name" value="Glyco_trans_51"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011815">
        <property type="entry name" value="PBP_1c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009647">
        <property type="entry name" value="PBP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036950">
        <property type="entry name" value="PBP_transglycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001460">
        <property type="entry name" value="PCN-bd_Tpept"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06832">
        <property type="entry name" value="BiPBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00912">
        <property type="entry name" value="Transgly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00905">
        <property type="entry name" value="Transpeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02073">
        <property type="entry name" value="PBP_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="7 15" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="Transgly" evidence="13">
        <location>
            <begin position="74"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Transpeptidase" evidence="12">
        <location>
            <begin position="320"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="BiPBP_C" evidence="14">
        <location>
            <begin position="620"/>
            <end position="704"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF06832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABA04795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="706" mass="76172" checksum="6A55E80642EE3B99" modified="2005-10-11" version="1">MTTPDTPATIVQPRRWRRVKLAAAMMAVVALVASGAVTGWIVSLGPLPLAKAHEVSTTVLDRNGKLLRAFAMADGRWRLPVDATTDVDPGYLNLLLGYEDQRFYSHAGIDPLALGRAARQLLTRGHIVSGGSTITMQLARLIEPRQRRSIYAKLRQMVRALQLERELTKGEILNLYLTLAPFGGNLEGIRAASLAYFGKEPKRLSLAQSALLVALPQSPERRRLDRHPAAARAARDRVLMRMVEDSRIGAEDARRAMAVPVLRTRRPFPMLAPHSADQALATNKDSNLIRLTLESGLQRQLETLARDRAAALGPDISVGIIAVDNATGDVVAHVGSADYFNERLAGQVDMTRAVRSPGSTLKPFIYGLAFEDGLVHPDSLIDDRPVRFGSYAPENFDMTFQGTVPVRRALQLSLNVPAIELLDRIGASRLSSRLKQAGASLVLPKDEAPGLAMGLGGVGVTLHDLVQAYSGIPRLGIVMPLREIEHDDSSVRSPLRLLDAVAAWQVGNVLLGTPPPENAVRNRIAFKTGTSYGYRDAWSVGFDGRMTIGVWVGRPDGAPVPGLIGRVAAAPILFDAFARTGRLPAPLPKAPRGTLIAANAKLPLPLQRFRSAGELIRIANRERRLHIQFPLNGSRIDAGAGQGTLSPLPVKVAGGSLPMTMLVNGKAVGAIDGRRQRMIDPPGPGFVRLTVMDAAGTTDTVTVRIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSK1</accession>
    <name>Q3SSK1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1479</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1479"/>
    <dbReference type="EnsemblBacteria" id="ABA04740">
        <property type="protein sequence ID" value="ABA04740"/>
        <property type="gene ID" value="Nwi_1479"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1479"/>
    <dbReference type="HOGENOM" id="CLU_2302943_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="100" mass="11275" checksum="689A8FA8DD0EB98F" modified="2005-10-11" version="1">MQNDEKHDRQLWCAVLGQAITDATTTRTGKDAQRDQHAAIQWLLTDTIDFPIVCTLAGVDPEAVRERFMRIPGVVEKFQGTQRDRRGSTTQDLTELGVFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSK6</accession>
    <name>Q3SSK6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Per-hexamer repeat gene protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1474</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314742.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1474"/>
    <dbReference type="EnsemblBacteria" id="ABA04735">
        <property type="protein sequence ID" value="ABA04735"/>
        <property type="gene ID" value="Nwi_1474"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1474"/>
    <dbReference type="eggNOG" id="ENOG503376F">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_794174_0_0_5"/>
    <dbReference type="OMA" id="FMDSIPW"/>
    <dbReference type="OrthoDB" id="2101756at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="349" mass="32154" checksum="768A34D9C2F75467" modified="2005-10-11" version="1">MFKLFDRVKVNIATTGTGDVTFGSASSNAFLTPTEAGAGDGDTVRYMIVDGLDFEEGIGTIGGGVTTMARTTVAKSKIGGTAGTSKINLSGTAVLSLTASAADILIPANNLSDLDDADTALDNLGGTTVGKALFTAADAAEARGTLEISDTLPTTQVFTTGSGTYTTPAGCKWIEVELIGGGGGGSGSGTSYGNGFAAGNTTFGDLTGGGANGGANAGRGTGGSASGGYVNLTGAGGGNGSGVNSTQGGSGGASPFGGAGAGGAAGAGAGTAAAANTGSGGGGGGVNTTPNGGGGGGSGGYVKAIINSPTSTYSYSVAAGGNGGSAGTGGAAGGAGGSGIIIVTEHYGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSM4</accession>
    <name>Q3SSM4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Secretion protein HlyD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1456</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314724.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1456"/>
    <dbReference type="EnsemblBacteria" id="ABA04717">
        <property type="protein sequence ID" value="ABA04717"/>
        <property type="gene ID" value="Nwi_1456"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1456"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018816_13_0_5"/>
    <dbReference type="OMA" id="NTFEVRM"/>
    <dbReference type="OrthoDB" id="1174564at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032317">
        <property type="entry name" value="HlyD_D23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16576">
        <property type="entry name" value="HlyD_D23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HlyD_D23" evidence="1">
        <location>
            <begin position="139"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="330" mass="35838" checksum="B8BAB15FAA88B5B4" modified="2005-10-11" version="1">MIKRFTIIIFAVVVLAVAGIGYRMLDLTDPGVPALREDHDHTIHEFKRGHVEEDEHGAKRILISDVKLAAAGAVLAEAGEVTLSDTLSFNGVLRANQETVVQVTPRFPGIVRSIRKRIGDTVAGNDLLASIESNQSLTVYDLKAPISGTVIDRQISLGEYASEQKPAFVVADLSTIWVDLSIYRQDLKRVRTGDEVLIDPDDGGADIKGHISYVAPIGSIDTQTAIARIVLTNTDGRLRPGLFVTARLVLTKRSVPVAVRAGAIQMLDNKTVVFVREDQDKIEARPVVVGESDARFVEIRAGMVAGEPYVAENSFVVKAEMGKGEGEHDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="131" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSN3</accession>
    <name>Q3SSN3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Two component, sigma54 specific, transcriptional regulator, Fis family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_1447</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314716.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1447"/>
    <dbReference type="EnsemblBacteria" id="ABA04708">
        <property type="protein sequence ID" value="ABA04708"/>
        <property type="gene ID" value="Nwi_1447"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1447"/>
    <dbReference type="eggNOG" id="COG2204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_0_6_5"/>
    <dbReference type="OMA" id="PTNSRVM"/>
    <dbReference type="OrthoDB" id="123059at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002197">
        <property type="entry name" value="HTH_Fis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002078">
        <property type="entry name" value="Sigma_54_int"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025943">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025944">
        <property type="entry name" value="Sigma_54_int_dom_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02954">
        <property type="entry name" value="HTH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00158">
        <property type="entry name" value="Sigma54_activat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00676">
        <property type="entry name" value="SIGMA54_INTERACT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00688">
        <property type="entry name" value="SIGMA54_INTERACT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50045">
        <property type="entry name" value="SIGMA54_INTERACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="10">
        <location>
            <begin position="4"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma-54 factor interaction" evidence="9">
        <location>
            <begin position="142"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="8">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA04708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="456" mass="50412" checksum="DF1100C87647A666" modified="2005-10-11" version="1">MAHDILIVDDEADIRDLVAGILEDEGFTTRTARNSDEALAEVSSRRPHMVFLDIWLQGSKLDGLQLLEMIKQDSPDMPVVMISGHGNIETAVAAIKRGAYDFIEKPFKADRLILVATRALETSRLKREVRELKQLAPAASSLVGRSPCMNQLRQTIERAAKANSRIMIVGPSGSGKELAARTLHNQSSRAEGPFVVINAAAITPERMESELFGVEQVDGQQPRKAGALEEAHGGTLFIDEIGDMPRETQNKIMRVLVDQTFQRSGGATKIKVDVRIISSTARNLEEEIAAGRFREDLYHRLSVVPIRVPPLSERREDIPELIEFFMDHISATSGLPKRQIGDDAMAVLQSHVWPGNVRQLRNNVERVMILAGGGPEVIITADMLPQDVGSMVPAMPSGVNGEHIMGLPLREAREVFERDYLIAQISRFSNNISRTAEFVGMERSALHRKLKALGVN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-12-16" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSP7</accession>
    <name>RL33_NITWN</name>
    <protein>
        <recommendedName>
            <fullName>50S ribosomal protein L33</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">rpmG</name>
        <name type="ordered locus">Nwi_1433</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL33 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_006611239.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSP7"/>
    <dbReference type="SMR" id="Q3SSP7"/>
    <dbReference type="STRING" id="323098.Nwi_1433"/>
    <dbReference type="EnsemblBacteria" id="ABA04694">
        <property type="protein sequence ID" value="ABA04694"/>
        <property type="gene ID" value="Nwi_1433"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1433"/>
    <dbReference type="eggNOG" id="COG0267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_190949_1_1_5"/>
    <dbReference type="OMA" id="GTGFYYT"/>
    <dbReference type="OrthoDB" id="2034291at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038584">
        <property type="entry name" value="L33_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001705">
        <property type="entry name" value="Ribosomal_L33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00471">
        <property type="entry name" value="Ribosomal_L33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01023">
        <property type="entry name" value="rpmG_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000356587" description="50S ribosomal protein L33">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="55" mass="6337" checksum="1D9A3102CC958E08" modified="2005-10-11" version="1">MAKAVTIKVKLVSSADTGFYYVAKKNSRTMTDKMVKKKYDPVARKHVEFREAKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSQ4</accession>
    <name>Q3SSQ4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycerophosphoryl diester phosphodiesterase</fullName>
            <ecNumber evidence="2">3.1.4.46</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1426</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.4.46" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314696.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1426"/>
    <dbReference type="EnsemblBacteria" id="ABA04687">
        <property type="protein sequence ID" value="ABA04687"/>
        <property type="gene ID" value="Nwi_1426"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1426"/>
    <dbReference type="eggNOG" id="COG0584">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030006_10_0_5"/>
    <dbReference type="OMA" id="ADQMIFE"/>
    <dbReference type="OrthoDB" id="1542724at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008889">
        <property type="term" value="F:glycerophosphodiester phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="SSF51695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GP-PDE" evidence="1">
        <location>
            <begin position="9"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="250" mass="27726" checksum="8E4799E02E179FB3" modified="2005-10-11" version="1">MRAPDWLTARPIAHRGLHDHARGIMENMPGAIRAAIAGNFAIEVDIQLTADNEAMVYHDDELGRLTEGSGALRTVTAAELKRVIFKSTPEKMMSLADLCALVAGRVPLVIEVKSHFDGGTDLVRRMAEVLSGYDGPAAGMSFDPDQVMAMRELMPSLPRGIVAERRYSKADWPEATSRQRAAMTHLRHGFRTRPHFVAYNVNELPALAPWIARNILGCPLLTWTVRSAEQRARAARHADQMIFEGFYPNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SST7</accession>
    <name>Q3SST7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1393</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1393"/>
    <dbReference type="EnsemblBacteria" id="ABA04654">
        <property type="protein sequence ID" value="ABA04654"/>
        <property type="gene ID" value="Nwi_1393"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1393"/>
    <dbReference type="eggNOG" id="COG4783">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1183000_0_0_5"/>
    <dbReference type="OMA" id="QPFFGFA"/>
    <dbReference type="OrthoDB" id="1423349at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="127"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="279" mass="31208" checksum="BF4EBC32B95140B0" modified="2005-10-11" version="1">MTGLIDAMVRLPIHDPDKFARRRTFKDCFRTERLMMLRQGARRNQVQTMRRHMTRWLLALALLGITNAARAEMAEVAPGVTVTKKTFQARMDEQPFYGFAEKTAEQQKADTDFLAAADRKGTREAVSAAAIERGWGHLDRGNFPEAIKRFNQAWLLAPERSAIYQGFAVVTAARFGDLDYAEELFKVALRQPKQRRTLKADYGRLLLKAKRPAEAKTILEEGVREAPDFADGWVSLGLARLQTDDAAGACAAATEANKLKLSRGAMIDLLLLKRQGACP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSY1</accession>
    <name>RL7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L7/L12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplL</name>
        <name type="ordered locus">Nwi_1349</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Is thus essential for accurate translation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Part of the ribosomal stalk of the 50S ribosomal subunit. Forms a multimeric L10(L12)X complex, where L10 forms an elongated spine to which 2 to 4 L12 dimers bind in a sequential fashion. Binds GTP-bound translation factors.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL12 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314628.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSY1"/>
    <dbReference type="SMR" id="Q3SSY1"/>
    <dbReference type="STRING" id="323098.Nwi_1349"/>
    <dbReference type="EnsemblBacteria" id="ABA04610">
        <property type="protein sequence ID" value="ABA04610"/>
        <property type="gene ID" value="Nwi_1349"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1349"/>
    <dbReference type="eggNOG" id="COG0222">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086499_3_0_5"/>
    <dbReference type="OMA" id="LEDKWGV"/>
    <dbReference type="OrthoDB" id="1822695at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00387">
        <property type="entry name" value="Ribosomal_L7_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00368">
        <property type="entry name" value="Ribosomal_L7_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000206">
        <property type="entry name" value="Ribosomal_L7/12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014719">
        <property type="entry name" value="Ribosomal_L7/12_C/ClpS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013823">
        <property type="entry name" value="Ribosomal_L7/L12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008932">
        <property type="entry name" value="Ribosomal_L7/L12_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036235">
        <property type="entry name" value="Ribosomal_L7/L12_oligo_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45987">
        <property type="entry name" value="PTHR45987"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00542">
        <property type="entry name" value="Ribosomal_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16320">
        <property type="entry name" value="Ribosomal_L12_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48300">
        <property type="entry name" value="SSF48300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54736">
        <property type="entry name" value="SSF54736"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00855">
        <property type="entry name" value="L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000243451" description="50S ribosomal protein L7/L12">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00368"/>
        </source>
    </evidence>
    <sequence length="125" mass="12727" checksum="66D849AD447BBC69" modified="2005-10-11" version="1">MADLQKIVDDLSALTVLEAAELAKLLEEKWGVSAAAAVAVAAAPGAGGGAPAAEEKTEFTVVLAAAGDKKIEVIKEVRAITGLGLKEAKDLVEGAPKPVKEGVAKDEAEKIKAQLEKAGGKVELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSZ3</accession>
    <name>Q3SSZ3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative outer membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1337</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314616.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1337"/>
    <dbReference type="EnsemblBacteria" id="ABA04598">
        <property type="protein sequence ID" value="ABA04598"/>
        <property type="gene ID" value="Nwi_1337"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1337"/>
    <dbReference type="eggNOG" id="COG3637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037100_0_1_5"/>
    <dbReference type="OMA" id="NYRFGWG"/>
    <dbReference type="OrthoDB" id="2018542at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027385">
        <property type="entry name" value="OMP_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13505">
        <property type="entry name" value="OMP_b-brl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229292" evidence="1">
        <location>
            <begin position="21"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="OMP_b-brl" evidence="2">
        <location>
            <begin position="10"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="241" mass="25168" checksum="DB8C0E22FC275191" modified="2005-10-11" version="1">MKKFLLTAVVATAFAAPALAADIAPRPYTKAPVPYTPPQAVYNWTGFYIGGHLGGAFGGDLGGSDGRFLGGVQGGADYQFANNWVLGIEAQHSWLANYRENVAFPGGSVVTGGNSGLGSVTGRLGYSWGPTLLYAKGGYAFRDGALGVTDAAGAPQAFTTTGNRNSGYTVGAGLEYMFAPNWSAKAEYQYFNFGRTTFGAGSPADVVGASFRNDEHTVKAGVNYRFGWGGPPAPQYPAPQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSZ7</accession>
    <name>Q3SSZ7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hpt protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1333</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314612.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1333"/>
    <dbReference type="EnsemblBacteria" id="ABA04594">
        <property type="protein sequence ID" value="ABA04594"/>
        <property type="gene ID" value="Nwi_1333"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1333"/>
    <dbReference type="eggNOG" id="COG2198">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121898_0_0_5"/>
    <dbReference type="OMA" id="CRVIEHA"/>
    <dbReference type="OrthoDB" id="2065147at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036641">
        <property type="entry name" value="HPT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008207">
        <property type="entry name" value="Sig_transdc_His_kin_Hpt_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01627">
        <property type="entry name" value="Hpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47226">
        <property type="entry name" value="SSF47226"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HPt" evidence="1">
        <location>
            <begin position="71"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="192" mass="21248" checksum="C4EFF215422A622B" modified="2005-10-11" version="1">MLTENSQELNIQAFADHHIITPPNRLRNAVRLVDEKNFDDPIARAEKALAGLSDEFSKWMEIERDRLVMAHAAILNNGFTPAARDELFRAAHDIKGDAATFGYPFAAAGAESLCRIIEHAPALDRVPAELIAYHINAIQAIVREHTTPGAEITAMEINRKLRLVADEYLAAVNQDRPEHLEAILAPSIAPTE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-21" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST14</accession>
    <name>RS4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsD</name>
        <name type="ordered locus">Nwi_1316</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="1">With S5 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Contacts protein S5. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314595.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ST14"/>
    <dbReference type="SMR" id="Q3ST14"/>
    <dbReference type="STRING" id="323098.Nwi_1316"/>
    <dbReference type="EnsemblBacteria" id="ABA04577">
        <property type="protein sequence ID" value="ABA04577"/>
        <property type="gene ID" value="Nwi_1316"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1316"/>
    <dbReference type="eggNOG" id="COG0522">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092403_0_0_5"/>
    <dbReference type="OMA" id="NVVFRMG"/>
    <dbReference type="OrthoDB" id="1211060at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01306_B">
        <property type="entry name" value="Ribosomal_S4_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_S4/S9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_S4/S9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005709">
        <property type="entry name" value="Ribosomal_S4_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018079">
        <property type="entry name" value="Ribosomal_S4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="PTHR11831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF4">
        <property type="entry name" value="PTHR11831:SF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01017">
        <property type="entry name" value="rpsD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00632">
        <property type="entry name" value="RIBOSOMAL_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000228905" description="30S ribosomal protein S4">
        <location>
            <begin position="1"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="S4 RNA-binding" evidence="1">
        <location>
            <begin position="94"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="18"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="34"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="205" mass="23603" checksum="D6A5749AF07F00E4" modified="2005-10-11" version="1">MTKRNEAKYKIDRRMGQNIWGRPKSPVNKREYGPGQHGQRRKGKLSDFGTQLRAKQKLKGYYGNISERQFYAIYVEATRMKGDSGENLIGLLERRLDTVVYRAKFVPTIFAARQFINHGHVKVNGRRVNIPSYKLKVGDTVEVKDASKQLALVLEANQLAERDVPDFIDADHNKQSAKFIRIPQLADVPFAVQMEPHLIVEFYSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST64</accession>
    <name>Q3ST64_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Secretion protein HlyD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_1266</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1266"/>
    <dbReference type="EnsemblBacteria" id="ABA04527">
        <property type="protein sequence ID" value="ABA04527"/>
        <property type="gene ID" value="Nwi_1266"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1266"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018816_2_0_5"/>
    <dbReference type="OMA" id="KVYFTEG"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032317">
        <property type="entry name" value="HlyD_D23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006143">
        <property type="entry name" value="RND_pump_MFP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16576">
        <property type="entry name" value="HlyD_D23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01730">
        <property type="entry name" value="RND_mfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="HlyD_D23" evidence="4">
        <location>
            <begin position="100"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="141"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="411" mass="44371" checksum="3D7377FD20EB828C" modified="2005-10-11" version="1">MVCLQRICAHIPRPRALFRSFEVFVQARALMPRSRVPGWTLLGAAIAVVASYALWHGGGKHNGPSRDAAQAPPVPVKIATVRKGDFPIYLTGLGTVQGFNTVVVRTRVDGQIERIAFKEGQLVEKGDLLAHIDRRPYQAALDQAKAKKAQDEANLANAELDLQRYTKLGEFATRKQTDTQRSMVKQLTAQIAADEAAIVNAQTQLDYTSIRAPIAGVTGFRLVDRGNIVNASTQTGIVTIAQIEPIAVIFTAPENQLPQIKDAITAGSPKTIAYSPDGKRMLSEGTLMLINNQVDTASGTIRLKAVFENKDHVLWPGQSVSTRLLVSTAKDAVVVPDDAIKHGTDGLYAYVVNKDNKAEFRRITVGKSIDGETIIEKGIAPGERVIVAGQYRVQPGSVVTETIASSGSSDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST72</accession>
    <name>Q3ST72_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1258</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314547.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1258"/>
    <dbReference type="EnsemblBacteria" id="ABA04519">
        <property type="protein sequence ID" value="ABA04519"/>
        <property type="gene ID" value="Nwi_1258"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1258"/>
    <dbReference type="eggNOG" id="COG3378">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025569_0_0_5"/>
    <dbReference type="OMA" id="VIKFRRC"/>
    <dbReference type="OrthoDB" id="358978at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="553"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="570"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="650" mass="69997" checksum="509E6EC8F8E48B64" modified="2005-10-11" version="1">MRSEIDGEDLNPIVDAIDAAEEISDPLDGLVEKSAEDPGAAFAPEVLERLVALKAEDRAAFEALRAKLKKAGCRVTALDEAMAEETGDGGRGPTQADILIDLAGGAELFHSADGTAFADIMINDHRETWPVRTKGFRRWLARQFFEQTGGAPSSEALQSALNVIEAKAHFDGPERVVFIRVGGQDERLYLDLGDEAWRAVEIDATGWRVVNDPPVRFRRAAGMQPLAVPMLGGSVETLRAFLNVQSDQDFVLVVAWALAVLRNKGPYPALVLSGEQGSAKSTFSAILRALLDPNTAPLRALPREDRDLFIAASNGHVLAFDNVSGLPAWISDTLCRLATGGGFAVRQLYTDQDEVLFDAARPVILNGIEDIVTRPDLADRAVFLTLEPIPEKRRRPEQELWAAFEAERPRLLGVLLDAVVEGLKRLPDTHLPKLPRMADFALWASACETALWPSGTFWSAYCGNRDEAVDGVIDADPVAAAVRAVMTERTEWTGTASDLLGALAEMAGERVAKSKTWPDSPRALSGRLRRAATFLRKIGIDIGFEREGRARTRTIRITTAGKPASPEIRGAQPSASSASSADRPKDSSGNGFASPGLRTVGEFADGSAGEPGATVRANMLKSNAEADADGADASRPPQSAPENTGWRVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STA4</accession>
    <name>Q3STA4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Glutamine-dependent NAD(+) synthetase</fullName>
            <ecNumber evidence="5 6">6.3.5.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5 6">NAD(+) synthase [glutamine-hydrolyzing]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">nadE</name>
        <name evidence="10" type="ordered locus">Nwi_1225</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses L-glutamine as a nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>ATP + deamido-NAD(+) + H2O + L-glutamine = AMP + diphosphate + H(+) + L-glutamate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:24384"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 5 6">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the NAD synthetase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5 6">In the C-terminal section; belongs to the NAD synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.1" evidence="5 6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314516.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1225"/>
    <dbReference type="EnsemblBacteria" id="ABA04487">
        <property type="protein sequence ID" value="ABA04487"/>
        <property type="gene ID" value="Nwi_1225"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1225"/>
    <dbReference type="eggNOG" id="COG0171">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0388">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022313_2_0_5"/>
    <dbReference type="OMA" id="VLMPSPY"/>
    <dbReference type="OrthoDB" id="1152435at2"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00334"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003952">
        <property type="term" value="F:NAD+ synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008795">
        <property type="term" value="F:NAD+ synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00553">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02090">
        <property type="entry name" value="NadE_glutamine_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014445">
        <property type="entry name" value="Gln-dep_NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003694">
        <property type="entry name" value="NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090">
        <property type="entry name" value="PTHR23090"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006630">
        <property type="entry name" value="NADS_GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="SSF56317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00552">
        <property type="entry name" value="nadE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 6 7" id="KW-0520">NAD</keyword>
    <keyword evidence="3 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="9">
        <location>
            <begin position="6"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="549"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for glutaminase activity" evidence="5">
        <location>
            <position position="46"/>
        </location>
    </feature>
    <feature type="active site" description="For glutaminase activity" evidence="5">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; for glutaminase activity" evidence="5">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="297"/>
            <end position="304"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="380"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="404"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="409"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="528"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR006630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA04487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="583" mass="62691" checksum="92BD702E8E825B8E" modified="2005-10-11" version="1">MTETTFKITLGQLNPTVGDIVGNAAKARAARAQAKADGAALVALPELFIAGYPPEDLVLRPAFQAACREAIEALARETSDGGPAMLIGTPWVDDGKLYNAYALLDEGRVAALRFKVNLPNYGVFDEKRVFARGPVGGPLKIRGVRIGVPICEDTWVEESAEYENVVECLAETGAEILVVPNGSPYAKGKSDIRLSISVARVTESDLPLVYLNQVGGQDELVFDGASFVLNADRSLAAQLPFFEESVFTLQWTRSAEGWRCAGPVAPQIDGDKADYAACVLGLRDYVRKNGFPGVLLGVSGGIDSALCAAIAVDALGAEQVRGVMLPFRFTAQVSLDDAARLATALGIRYDVLPIAQAVNGFEEILSGVFAGAPRDITEENLQSRARGTLLMAMSNKLGAMVVTTGNKSEMSVGYATLYGDMNGGFNPIKDIYKTEVLRMASLRNAWKPDGALGPSGEVIPPGAISRPPTAELREGQLDQDSLPPYDLLDAILERLVEREEPLAAIVAEGFDHDVVVRVDRLLNAAEYKRRQAAPGVKVTRRNFGRDRRYPITNRFRDSGESLPAPDETLVSRGSRSSAEAFQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STF8</accession>
    <name>Q3STF8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Peptidase S1 and S6, chymotrypsin/Hap</fullName>
            <ecNumber evidence="3">3.4.21.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1171</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.4.21.4" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1171"/>
    <dbReference type="EnsemblBacteria" id="ABA04433">
        <property type="protein sequence ID" value="ABA04433"/>
        <property type="gene ID" value="Nwi_1171"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1171"/>
    <dbReference type="eggNOG" id="COG5640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079867_0_0_5"/>
    <dbReference type="OMA" id="FPWLLSC"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="SSF50494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229301" evidence="1">
        <location>
            <begin position="28"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="2">
        <location>
            <begin position="28"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="255" mass="25448" checksum="6CBBB10DF4ED53B9" modified="2005-10-11" version="1">MCPPMTFFIRNGIAAMLILVALSPASAMVGGGSAPSPALANAVVTIVGSQGNSCTGTLIARDIILTAAHCIAPGVATKMVDYGSQPPRLLDIRRAAVHPQFNPQSVLAHRATADVGLLQLAAPLAVQPVRIGGALMPINVGQRLTVVGMGVATPGDGRSGGVARAASLAVTGKPGTLQIRLTDPATNNSRAGLGACTGDSGAPAMLDSGGVIVGVVSWSTGANNASGCGGLTGVTPLALYRDWILRTARSWGAGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STG2</accession>
    <name>Q3STG2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phage major capsid protein, HK97</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1167</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314458.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1167"/>
    <dbReference type="EnsemblBacteria" id="ABA04429">
        <property type="protein sequence ID" value="ABA04429"/>
        <property type="gene ID" value="Nwi_1167"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1167"/>
    <dbReference type="eggNOG" id="COG4653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041417_0_0_5"/>
    <dbReference type="OMA" id="GDFRRGY"/>
    <dbReference type="OrthoDB" id="755947at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024455">
        <property type="entry name" value="Phage_capsid"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05065">
        <property type="entry name" value="Phage_capsid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01554">
        <property type="entry name" value="major_cap_HK97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="417" mass="44832" checksum="DF8F7283493F3A9D" modified="2005-10-11" version="1">MDFDITDMAPEHKSGGAVRGGYDDFRVTFEEFKAANDERLARLEQKRGDVLLEEKVDRINAALDAHHRRMDELALKHARPALEGRSGIVGGAAALEHKSAFEAYVRGGETGALRALETKAMSAGSNPDGGYLVPVELEHEIGQRLAAISPIRALASVRTISGNVYKKPFMTAGPATGWVGETDSRTQTTSPTLDALSFPAMELYAMPAATATLLDDSAVNIDEWIAQEVELTFAVQEGAAFVNGDGTNKPKGFLQSETVANGSWAWGKLGFVASGGASGFASSNPSDALVDLVYALKAGYRQNAAFIMNRKTQAAIRKFKDTGGSYLWQPPAQAGGRASLMTFPLVEAEDMPDVAENSLSIAFGDFRRGYLVVDRAGVRVLRDPYSAKPYVLFYTTKRVGGGVQDFDAIKLMKFAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STG6</accession>
    <name>Q3STG6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1163</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314454.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1163"/>
    <dbReference type="MEROPS" id="S78.001"/>
    <dbReference type="EnsemblBacteria" id="ABA04425">
        <property type="protein sequence ID" value="ABA04425"/>
        <property type="gene ID" value="Nwi_1163"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1163"/>
    <dbReference type="eggNOG" id="COG3740">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073043_2_1_5"/>
    <dbReference type="OMA" id="WEYDRTK"/>
    <dbReference type="OrthoDB" id="1734449at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006433">
        <property type="entry name" value="Prohead_protease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04586">
        <property type="entry name" value="Peptidase_S78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01543">
        <property type="entry name" value="proheadase_HK97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-1188">Viral release from host cell</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="151"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="215" mass="24052" checksum="45D89AC3E05DE75F" modified="2005-10-11" version="1">MHAPLSSVSRLSFFSDGMVEGYASLFGEIDQARDMMMPGAFTQTLKTRGLRRIPMLFQHDPAEPVGVWLELHEDFRGLWARGKLIPDVTRARELLSLVEAGAVDGLSIGYRTVRGRIEPRTRVRHLHQVDLWEISIVTFPLLAGARVRAVKQATPPSPRSRARTQAEREWRSMRQAADEPAPSLRPRPVVTSLRGREGRRVALCGAMRAPLAGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STP3</accession>
    <name>Q3STP3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1086</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314382.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1086"/>
    <dbReference type="EnsemblBacteria" id="ABA04348">
        <property type="protein sequence ID" value="ABA04348"/>
        <property type="gene ID" value="Nwi_1086"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1086"/>
    <dbReference type="eggNOG" id="COG1196">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000496_0_0_5"/>
    <dbReference type="OMA" id="KYDRVDP"/>
    <dbReference type="OrthoDB" id="86909at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042157">
        <property type="term" value="P:lipoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000074">
        <property type="entry name" value="ApoA_E"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01442">
        <property type="entry name" value="Apolipoprotein"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="104"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1593"/>
            <end position="1692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1799" mass="191805" checksum="882E7A227143F549" modified="2005-10-11" version="1">MANNPKKVKDPTEVALSAIQEALSVGETSAAVDLDQVAARHDGAPPVPPASHVDDRPGAHASIDRPVLETAEGSRFSRRPANDDRETIGQILQTLQKSGPSRNVYTMATLFAGIWILGAGLLTISFLPSLQAALGQGSGGTLALAGLIALFFAPVLLFYFLAHMAWRGQELRMIAQSMAQMAIRFSEPEGLASDSVVTVGQAIRREVAAMGDGVERAIARAGELEALVANEVAALERAYSDNEVRIRALLQDIAHQRDNLVGQAEQVRNAISGVQIDLRQDIELISDAIASRVEEVAKGITGALEERGDHITTALSNAGDNMIFALGERGGDLLDRLEEASAQTARAVLDASERLTSSLNFKTGHVHDEFVDLADRVHDMLNERIDRITGEFEQKSASIVDSISDRTEQVHDALKNSSESLLLELELRGSDLASKVDDAGNRLASRILSCGDKASEALDVTVNSLVANVVSQTETAHDTLSLQMNAFDQLVKDQGAELAEKFARDSGTLGALITRHISEFDRTVKTFGAEIVDRLGQRTEDIAQSMKNYVDTFDTRLTSNGGEISAVLDQHLARFETSVRSRVTNLDSAFDAKIKSFDESVNGRLKTLEQIFDTRALSVTQTIDSRLVTLTSSLSDGAAHAIGSIESRLNHLTTSLTEGATQAVVALEGRISGVTDAIDDRSAYLADTITARFQEIHQGLDERVSFVATAIDTRVAQFEDLLGSRVEAVAGRFESSGREASDILLARAEELSFGIKSHVEDAERSLTNLVVNTSETIQTGTRSAQQALLSISTDVGAQLQLTSTEVERALTTAGTAAADSILSSAREAQTTLINSSAETASQVKSLSAEIEQTLTAAGTSTADSVLAGARAAHTTLVSASSDAANQVKSLASEIERTLSTVGSDVERMLSAAGTSTADTVLSSAMEAQTTLVKASTDTASQVKSLGSDIERTLTAVGADTAAAILSSAREAQGTLTTTSTEAVNQVKSLASDIEQTLTTVSAGTAAAILNSAREAQSTLTSTSADTARQVRSLASDIEQTLTTVGADTAAAILNSAREAQTTLTSTSADTASQVKSLASDIEQTLTTVGAETAAALLGSAREVQSTLTAASADSANQVKVISSDIERTLHAAAANTTEIIQASAQSAQTSLVSASNEISIKVKSVSTDAERLIFAASGSFGTTITGKTDEIVNYVQQQTDRLSQMLDSKRGSLIEALGSKADQLNSDIGRVTDDALKTIEAKGENFSQSIAANGSNVVRTITSAGELATGAISKSLKDLEEASRSAIDQSRQASIAAVTEMQETSKILRTDTVALFERLREGNILLQEVLTGAHENLNSLERTLVTRVADFVSTMREVTSQSGLATQSLEEQLTTFNTKTSKALDDLGSLSTQFETHGKALIEAAARVEQSNQKTTTSVEERKSALESLVTTIDLRTTDFDQRLTRFTSLLDESLAAAEERARDIARVVAETAGAGSAAINSQFEAVRAAAENERRLTSESMSEICKKAGQEAESMLRQSADRFAALVQSMKQMTAEMHRELETTRGELRRGVLEMPQEAAESTAQMRKVIVDQIEALAELNRIVARHGHGFDVVSSGRSSSQREAEPVMAAAGRSAAKVRSASRMRDNGNTAPTPAPGAQPARRTEQTAKPPVADGDGWLSDLLNDADTAERDVPRGRQASPAAAPANPLDSLSLDIGRLMDRKLVGEMWDRYQRGERNAFTKRLYTPAGQKAFDEVARKYRTDRAFKQTADRYVAEFERLLDEVARDEREPSVIRDHLISETGLVYTLLAHAAGRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STR3</accession>
    <name>Q3STR3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lipopolysaccharide heptosyltransferase I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1066</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314362.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1066"/>
    <dbReference type="CAZy" id="GT9">
        <property type="family name" value="Glycosyltransferase Family 9"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04328">
        <property type="protein sequence ID" value="ABA04328"/>
        <property type="gene ID" value="Nwi_1066"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1066"/>
    <dbReference type="eggNOG" id="COG0859">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038371_6_0_5"/>
    <dbReference type="OMA" id="ITHLAWA"/>
    <dbReference type="OrthoDB" id="1326265at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008920">
        <property type="term" value="F:lipopolysaccharide heptosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009244">
        <property type="term" value="P:lipopolysaccharide core region biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03789">
        <property type="entry name" value="GT9_LPS_heptosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002201">
        <property type="entry name" value="Glyco_trans_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011908">
        <property type="entry name" value="LipoPS_heptosylTferase-I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01075">
        <property type="entry name" value="Glyco_transf_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02193">
        <property type="entry name" value="heptsyl_trn_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="314" mass="34315" checksum="D3E17FE702598BC6" modified="2005-10-11" version="1">MTDVLIIKTSSLGDVVHQMPAIVDAARACPRLRLTWLVEEAFAPLVRLHPSVADVIPVATRRWRSQLGAIATWREIGAFRARLRRQAFEKVIDTQGLIRSAIMARVSRGARHGYDARSIREPLAARFYDVRHTVGRDLHAVTRNRLLTGLSLGYDPTGDVDYGLRSPARTEGQRYAVLLHGTSRLSKTWRAEDWIETGRWLQANGLQTVLPWGTEAERLLSERLSGEIAGSRVQPRQSLDLTARMIGNAALVIGVDTGLMHLAAAYRVPLIGIYVSTDPGLTGPMGAGRMAVLGGKSGPPPAGQVIDAAERLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU05</accession>
    <name>Q3SU05_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Molybdenum cofactor guanylyltransferase</fullName>
            <shortName evidence="4">MoCo guanylyltransferase</shortName>
            <ecNumber evidence="4">2.7.7.77</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">GTP:molybdopterin guanylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Mo-MPT guanylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Molybdopterin guanylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Molybdopterin-guanine dinucleotide synthase</fullName>
            <shortName evidence="4">MGD synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">mobA</name>
        <name evidence="6" type="ordered locus">Nwi_0974</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Transfers a GMP moiety from GTP to Mo-molybdopterin (Mo-MPT) cofactor (Moco or molybdenum cofactor) to form Mo-molybdopterin guanine dinucleotide (Mo-MGD) cofactor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>GTP + H(+) + Mo-molybdopterin = diphosphate + Mo-molybdopterin guanine dinucleotide</text>
            <dbReference type="Rhea" id="RHEA:34243"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
            <dbReference type="ChEBI" id="CHEBI:71310"/>
            <dbReference type="EC" id="2.7.7.77"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="4">The N-terminal domain determines nucleotide recognition and specific binding, while the C-terminal domain determines the specific binding to the target protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MobA family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.77" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314276.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0974"/>
    <dbReference type="EnsemblBacteria" id="ABA04236">
        <property type="protein sequence ID" value="ABA04236"/>
        <property type="gene ID" value="Nwi_0974"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0974"/>
    <dbReference type="eggNOG" id="COG0746">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055597_5_0_5"/>
    <dbReference type="OMA" id="IDFWYAK"/>
    <dbReference type="OrthoDB" id="2019898at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061603">
        <property type="term" value="F:molybdenum cofactor guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02503">
        <property type="entry name" value="MobA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00316">
        <property type="entry name" value="MobA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013482">
        <property type="entry name" value="Molybde_CF_guanTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02665">
        <property type="entry name" value="molyb_mobA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 4" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transf_3" evidence="5">
        <location>
            <begin position="17"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="20"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA04236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="218" mass="23370" checksum="7CDF3AE4C0831023" modified="2005-10-11" version="1">MNSTDAASPPDYPPTPAVILAGGLARRIGGGDKPMREIGGQTILNRVIARLASQCSGLILNANGDPARFSGFGLPVVADSVADFPGPLAGILAALDWVAEQRSGIQWIVSAPGDCPFLPRDLAARLHEVRDQQDAELAVAVSGGRTHPVIGLWNVRLRDELRRALTTEGLRKVDRWTARYRLAAVSWPTAPFDPFFNINTTDDLAEAERLVAQDNDGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU07</accession>
    <name>Q3SU07_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0007</name>
        <name evidence="2" type="ordered locus">Nwi_0181</name>
        <name evidence="3" type="ordered locus">Nwi_0972</name>
        <name evidence="4" type="ordered locus">Nwi_1283</name>
        <name evidence="5" type="ordered locus">Nwi_1591</name>
        <name evidence="6" type="ordered locus">Nwi_2474</name>
        <name evidence="7" type="ordered locus">Nwi_2499</name>
        <name evidence="8" type="ordered locus">Nwi_2823</name>
        <name evidence="9" type="ordered locus">Nwi_2862</name>
        <name evidence="10" type="ordered locus">Nwi_3014</name>
        <name evidence="11" type="ordered locus">Nwi_3038</name>
        <name evidence="12" type="ordered locus">Nwi_3115</name>
    </gene>
    <organism evidence="3 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="3 13" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="3">Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0007"/>
    <dbReference type="EnsemblBacteria" id="ABA03275">
        <property type="protein sequence ID" value="ABA03275"/>
        <property type="gene ID" value="Nwi_0007"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03449">
        <property type="protein sequence ID" value="ABA03449"/>
        <property type="gene ID" value="Nwi_0181"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04234">
        <property type="protein sequence ID" value="ABA04234"/>
        <property type="gene ID" value="Nwi_0972"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04544">
        <property type="protein sequence ID" value="ABA04544"/>
        <property type="gene ID" value="Nwi_1283"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04852">
        <property type="protein sequence ID" value="ABA04852"/>
        <property type="gene ID" value="Nwi_1591"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05727">
        <property type="protein sequence ID" value="ABA05727"/>
        <property type="gene ID" value="Nwi_2474"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05752">
        <property type="protein sequence ID" value="ABA05752"/>
        <property type="gene ID" value="Nwi_2499"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06076">
        <property type="protein sequence ID" value="ABA06076"/>
        <property type="gene ID" value="Nwi_2823"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06112">
        <property type="protein sequence ID" value="ABA06112"/>
        <property type="gene ID" value="Nwi_2862"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06264">
        <property type="protein sequence ID" value="ABA06264"/>
        <property type="gene ID" value="Nwi_3014"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06288">
        <property type="protein sequence ID" value="ABA06288"/>
        <property type="gene ID" value="Nwi_3038"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06362">
        <property type="protein sequence ID" value="ABA06362"/>
        <property type="gene ID" value="Nwi_3115"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0007"/>
    <dbReference type="KEGG" id="nwi:Nwi_0181"/>
    <dbReference type="KEGG" id="nwi:Nwi_0972"/>
    <dbReference type="KEGG" id="nwi:Nwi_1283"/>
    <dbReference type="KEGG" id="nwi:Nwi_1591"/>
    <dbReference type="KEGG" id="nwi:Nwi_2474"/>
    <dbReference type="KEGG" id="nwi:Nwi_2499"/>
    <dbReference type="KEGG" id="nwi:Nwi_2823"/>
    <dbReference type="KEGG" id="nwi:Nwi_2862"/>
    <dbReference type="KEGG" id="nwi:Nwi_3014"/>
    <dbReference type="KEGG" id="nwi:Nwi_3038"/>
    <dbReference type="KEGG" id="nwi:Nwi_3115"/>
    <dbReference type="eggNOG" id="COG3293">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055261_2_1_5"/>
    <dbReference type="OMA" id="RRMEHQN"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025161">
        <property type="entry name" value="Put_IS4/IS5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13340">
        <property type="entry name" value="DUF4096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA06076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA06112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA06264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA06288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA06362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="132" mass="15192" checksum="FDF0E7BCA38EA892" modified="2005-10-11" version="1">MILTRSCTMGIKRYELSDHQWAKIAPLLPGKASDPGRTGSDNRLFVNGCLWVLRSGAHWCDLPERYGRWKTVHRRFSRWCHAGVWERVFSTLTADRDNQYLMLDSTIVRAHQQAATGKGGPRIRRWGVPEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU39</accession>
    <name>Q3SU39_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0940</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314243.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0940"/>
    <dbReference type="EnsemblBacteria" id="ABA04202">
        <property type="protein sequence ID" value="ABA04202"/>
        <property type="gene ID" value="Nwi_0940"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0940"/>
    <dbReference type="eggNOG" id="COG4223">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065356_0_0_5"/>
    <dbReference type="OMA" id="WVVVTRQ"/>
    <dbReference type="OrthoDB" id="1835973at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="142"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="183"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="350" mass="36508" checksum="2FA9AD548A71E646" modified="2005-10-11" version="1">MSDHSSDRTAGERDNKLPEMGGKPSRGEPPKLAPEQELSAPSPDDNKPVADQVDLAIEAETEAEAPAIKALRGEAEAPAVEISPVEPEVEVEHSRSGLPRELGKAMVVTPFHERAWDHEAGDHGPAAADGARTSSRFGKRRFAAVAAMAALAAITGAIGGSLATSALRHGIADQQSSAVADRTQALEATIKQLESEIASLKTGVDRSARNDASRFAKVNDRLDKIEKAQADSAARLTKLHETLDKPHSAAAPAAPPAPAAVTAAANVTGSIPVAEPVPLPSPKPGIARLPTVQGWVLHDVMDGIALIEGRSGVFEVRSGDPVPGLGRVDAIRKQDGRWVVVTSRGLIGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU40</accession>
    <name>Q3SU40_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sodium/proton antiporter, NhaD family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0939</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314242.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0939"/>
    <dbReference type="EnsemblBacteria" id="ABA04201">
        <property type="protein sequence ID" value="ABA04201"/>
        <property type="gene ID" value="Nwi_0939"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0939"/>
    <dbReference type="eggNOG" id="COG1055">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_590412_0_0_5"/>
    <dbReference type="OMA" id="YFMYRNK"/>
    <dbReference type="OrthoDB" id="522940at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045016">
        <property type="entry name" value="NhaD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43269">
        <property type="entry name" value="PTHR43269"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="249"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="283"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="339"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="381"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="406" mass="43282" checksum="AF9DA33E96781C2D" modified="2005-10-11" version="1">MADPILIVGVPLDFIFFAFILLGIALFHRHTLPIALTGLAVIIAYKTGFTGFKSGAGLNGFAHHMAHEWVTLANLFLLLMGFALLSRHFEESRIPDAMPALLPDDWKGGVVLLVIVFILSSFLDNIAGALIGGTVARHVFRGKVHIGYLAAIVAASNAGGAGSVVGDTTTTMMWIDGVSPLSVLEAYIAAIVALAIFAVPAAMRQHRFSPIVKDPRRGVRVDGARVFVVAVILIAALAANVTANLKFPALLDTLPVLGLAVWAAILLTAFLRRPDWKVMPETFKGTVFLLALVTAASMMPVETLPAASWQTTLGLGFVSSVFDNIPLTELALKQGGYDWGFLAYAVGFGGSMIWFGSSAGVALSNMYPEAKSVGLWVRNGWTIAVAYVVGFFVMLAILGWHPDAPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUF8</accession>
    <name>Q3SUF8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0818</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0818"/>
    <dbReference type="EnsemblBacteria" id="ABA04083">
        <property type="protein sequence ID" value="ABA04083"/>
        <property type="gene ID" value="Nwi_0818"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0818"/>
    <dbReference type="eggNOG" id="COG0817">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143511_0_0_5"/>
    <dbReference type="OMA" id="FLRFKRW"/>
    <dbReference type="OrthoDB" id="1671964at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="177" mass="18714" checksum="12C2AF47C79E62F7" modified="2005-10-11" version="1">MLALAFENPAIPPAPAVAPPASRTILALDLGTRTGWAVLPRSGRIASGVTEFRPGRFEGAGMAFLRFEKFLADLNRDAGPFDAVVFEEVRAHAGTLAAQVYGGFLAHLTAWCERKAAPYLGVPVATIKRHATGKGNAPKEDVIRAVRAQGFLPKDDNEADALALLAWAIANEIGGAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUN2</accession>
    <name>Q3SUN2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transcriptional regulator, BadM/Rrf2 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0743</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314063.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0743"/>
    <dbReference type="EnsemblBacteria" id="ABA04009">
        <property type="protein sequence ID" value="ABA04009"/>
        <property type="gene ID" value="Nwi_0743"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0743"/>
    <dbReference type="eggNOG" id="COG1959">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107144_2_1_5"/>
    <dbReference type="OMA" id="AQEAFYA"/>
    <dbReference type="OrthoDB" id="1765806at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000944">
        <property type="entry name" value="Tscrpt_reg_Rrf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33221">
        <property type="entry name" value="PTHR33221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02082">
        <property type="entry name" value="Rrf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00738">
        <property type="entry name" value="rrf2_super"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51197">
        <property type="entry name" value="HTH_RRF2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="150" mass="16154" checksum="3AC9520A68557FC6" modified="2005-10-11" version="1">MRLTTYSDYALRVLMYLALKSDGLSTIAEIAGSYNVSEAHLMKVVHQLGVAGYIETVRGRGGGLRLARPVEAIGLAEVIRMTEPDMAVVSCLKPLNAPCKINPCCVLKRAMERAQQAFMDVLEGYTLADLVVPSGRLSNLLGISPTASTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUN5</accession>
    <name>Q3SUN5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0740</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0740"/>
    <dbReference type="EnsemblBacteria" id="ABA04006">
        <property type="protein sequence ID" value="ABA04006"/>
        <property type="gene ID" value="Nwi_0740"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0740"/>
    <dbReference type="eggNOG" id="ENOG5032SYQ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088562_1_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="200" mass="21236" checksum="8FC1F446983E4669" modified="2005-10-11" version="1">MMTAAPSRSRAERWVNGGERIAVLKRFLHANRFPLRPKRDRIDPPSFRPRKDRVMTGYLKLLGAVTASGLLGLSALPADALTAQECSAKYQAAKSAGTLDGQKWNDFRKAHCGAAATAPEAAEASAPTTAAAPSATSADNAVFPSAVSSKYAGESAGKARRHTCLDQYKANKSSNANGGMKWIMKGGGYYSECTKRLKGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUT2</accession>
    <name>Q3SUT2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0693</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03959">
        <property type="protein sequence ID" value="ABA03959"/>
        <property type="gene ID" value="Nwi_0693"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0693"/>
    <dbReference type="eggNOG" id="ENOG5032K9C">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062809_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="290"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="374" mass="39730" checksum="2EB2D71D291A4314" modified="2005-10-11" version="1">MLTEMPLRTATVPWMKSATTDLWVTRFPSFLPYTFHLAALLMPNGQLLSRHEDAARTGPCNTQPGTPMLFRSQPAGSQADPHEAIVAQLRDLAPRIKQEPFQLEETPLSTESFLPMGEPSFQAAPSNDNAAALQDAIPRARSRRGVVTVLLAICIGIGGAAAWHSYGEQAKQGLAQLVPQLLMEAPAPPQNAHAAEAEDTAATQAAMPQPQPTAEAAPPPEAATIGPEAPPPPTDFITTAPQAPPAQAALPAELAQSLETMANDIAALKSAIEDLKAGQQQLRREIAAAAEREAHPKPAPQRAKPASSQRQRTSPQAAVPPPPSRPSPPPPEATERQLPPPQRDAYIASQAPVPARLPPRPGDDNAPRPPMPLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUT9</accession>
    <name>Q3SUT9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flavin reductase-like, FMN-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0686</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314008.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0686"/>
    <dbReference type="EnsemblBacteria" id="ABA03952">
        <property type="protein sequence ID" value="ABA03952"/>
        <property type="gene ID" value="Nwi_0686"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0686"/>
    <dbReference type="eggNOG" id="COG1853">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059021_2_1_5"/>
    <dbReference type="OMA" id="HFCTGIA"/>
    <dbReference type="OrthoDB" id="1681849at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016646">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002563">
        <property type="entry name" value="Flavin_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01613">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00903">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavin_Reduct" evidence="1">
        <location>
            <begin position="23"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="193" mass="20124" checksum="3492B1896D682957" modified="2005-10-11" version="1">MNLAVRDISRGREIGSGEFRNAMRHLAGGVSVITAGRGDDISGMTVTSVASLAIEPATLTVAINRKASTLPLLLRYGAFGVNILTGGQAGIAERFTGKDGHKGAARFAGSSWTTGAFGVPLLKDALVAITCELEETIERHSHVIALGRVVHLAASEGDSSLAYWRGDYLAIERISGISRTAGGPLPTVHASDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV36</accession>
    <name>Q3SV36_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized Fe-S protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0588</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313916.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0588"/>
    <dbReference type="EnsemblBacteria" id="ABA03855">
        <property type="protein sequence ID" value="ABA03855"/>
        <property type="gene ID" value="Nwi_0588"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0588"/>
    <dbReference type="eggNOG" id="COG3217">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028286_5_0_5"/>
    <dbReference type="OMA" id="FPGDRLY"/>
    <dbReference type="OrthoDB" id="1885467at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005302">
        <property type="entry name" value="MoCF_Sase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005303">
        <property type="entry name" value="MOSC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03473">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03476">
        <property type="entry name" value="MOSC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="SSF50800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51340">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MOSC" evidence="1">
        <location>
            <begin position="102"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="258" mass="28148" checksum="21716EC9B0A6F4F4" modified="2005-10-11" version="1">MSGSSKATVAALYRYPVKGLSAEPLRHTTLTPGGTVPGDRSYAIENGPTGFDPAAPRYFPKSRFLMLMRDERLATLESRYDDASRVLTILTGGEEAARADLGTVEGRAAIERFFATTFAQELKGPPRVLVGNGHSFSDVARKVVSIINLDSVAAIEQMVGQPVHPLRFRANLYVRGWPAWSELDRLGHTLAIGDIRLKVVKRIVRCTATNVDPETGARDLDIPNALARTLGHRDCGIYAEVVTGGTVRIEDGIETFRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV59</accession>
    <name>Q3SV59_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, MarR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0565</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313893.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0565"/>
    <dbReference type="EnsemblBacteria" id="ABA03832">
        <property type="protein sequence ID" value="ABA03832"/>
        <property type="gene ID" value="Nwi_0565"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0565"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089893_3_0_5"/>
    <dbReference type="OMA" id="CHTCRYL"/>
    <dbReference type="OrthoDB" id="1744037at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="189" mass="20184" checksum="B2183104245135F3" modified="2005-10-11" version="1">MFSRTALEAAYLISRLDRLARSGVNVEGLNPAQWEALRYLSRANRFSRTPAALAGYAGSTRGTISQTLIALEQKGLASRQSSRRDGRSVELALTRRGEAVLRDDPLRRLADDIAAATGGDAGALLATLQDILSSTIARNGGRAFGVCRTCRHFRHNAKPGSASPHHCNALGESLSDSDSGAICQEHEPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV63</accession>
    <name>Q3SV63_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0561</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313889.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0561"/>
    <dbReference type="EnsemblBacteria" id="ABA03828">
        <property type="protein sequence ID" value="ABA03828"/>
        <property type="gene ID" value="Nwi_0561"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0561"/>
    <dbReference type="eggNOG" id="COG1014">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_952089_0_0_5"/>
    <dbReference type="OMA" id="RMAYEDP"/>
    <dbReference type="OrthoDB" id="1049886at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="294" mass="33221" checksum="442B48A28DBE1106" modified="2005-10-11" version="1">MNFGRSKTADVLPAIRLMFAKVSGESNNPIPTLPADMPENARLVAVDGVKRLIDYQGPGYAQLYVDRLRRFIGRRNIDDKVFADIARLMAARMAYDDPLRMAQLKLAEIRKGPHVSSIDVRKLYLDELTSLLPKRGADPMIRVLDYLGWRRLPVTMRFSTASGFRLRCLRILASLRRWRLSSLRYANERVWVERWLHMIDRSLAKQPAAVAAVVSTAEMVQGYGAAYAQNLRDWNLIIDHLVKPTFDGAVLCPDLGAAIAEARAAVEHDPRQDSLKRVIAGLRERTGADDRRAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVB3</accession>
    <name>Q3SVB3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphate regulon transcriptional regulatory protein PhoB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_0511</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by PhoB and PhoR when phosphate is limited.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313839.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0511"/>
    <dbReference type="EnsemblBacteria" id="ABA03778">
        <property type="protein sequence ID" value="ABA03778"/>
        <property type="gene ID" value="Nwi_0511"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0511"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_4_5"/>
    <dbReference type="OMA" id="IDRVWGS"/>
    <dbReference type="OrthoDB" id="817710at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000156">
        <property type="term" value="F:phosphorelay response regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011879">
        <property type="entry name" value="Sig_transdc_resp-reg_PhoB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02154">
        <property type="entry name" value="PhoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Response regulatory" evidence="8">
        <location>
            <begin position="4"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="9">
        <location>
            <begin position="129"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="7">
        <location>
            <begin position="129"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="6">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA03778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="234" mass="26635" checksum="7BBC68DB5FC09A2D" modified="2005-10-11" version="1">MGARILVVEDEEALTTLLRYNLEAEGYEVETVVRGDEADTRLKERVPDLVVLDWMLPGLSGIELCRRLRARSDTRQLPIIMLTARGEESERVRGLATGADDYVVKPFSVPELLARVKSLLRRASPARLASVLSFGDIELDRDRHRVVRSGRPIDLGPTEYRLLEFFLEHPGRVFSREQLLDSVWGRDVYIDERTVDVHIGRLRKLLNPDGELDPIRTVRGAGYALDEQFASRTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVB4</accession>
    <name>Q3SVB4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphate-specific transport system accessory protein PhoU</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0510</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Plays a role in the regulation of phosphate uptake.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the PhoU family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313838.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0510"/>
    <dbReference type="EnsemblBacteria" id="ABA03777">
        <property type="protein sequence ID" value="ABA03777"/>
        <property type="gene ID" value="Nwi_0510"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0510"/>
    <dbReference type="eggNOG" id="COG0704">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078518_2_1_5"/>
    <dbReference type="OMA" id="WKHGIET"/>
    <dbReference type="OrthoDB" id="1648549at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:cellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045936">
        <property type="term" value="P:negative regulation of phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028366">
        <property type="entry name" value="P_transport_PhoU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930">
        <property type="entry name" value="PTHR42930"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01895">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003107">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02135">
        <property type="entry name" value="phoU_full"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PhoU" evidence="4">
        <location>
            <begin position="22"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="PhoU" evidence="4">
        <location>
            <begin position="125"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR003107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="238" mass="26636" checksum="834B974C35AF1C7A" modified="2005-10-11" version="1">MAFEHTAKAFDDDLQELTRLVSEMGGLAERQIVESLDALIRRDTTLGDRVVAADSEIDQLQRMIEERAVLTIARRQPMAVDLREIVGAMRVAIDLERIGDLAKNMGKRVVAIDSDFRPLKLIRGLEHMTDLVRSQVKSVLDAYAAHDLPAAMAVWKGDEEVDAICTSLFRELLTYMMEDPRNIGFCIHLMFCAKNIERIGDHATNIAETVFYMIEGQQILDKRPKGDMTGFASAAPGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVB6</accession>
    <name>Q3SVB6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Phosphate transport system permease protein PstA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_0508</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Cell inner membrane</location>
            <topology evidence="2 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313836.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0508"/>
    <dbReference type="EnsemblBacteria" id="ABA03775">
        <property type="protein sequence ID" value="ABA03775"/>
        <property type="gene ID" value="Nwi_0508"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0508"/>
    <dbReference type="eggNOG" id="COG0581">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033621_2_0_5"/>
    <dbReference type="OMA" id="GIARWKA"/>
    <dbReference type="OrthoDB" id="1763210at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005672">
        <property type="entry name" value="Phosphate_PstA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00974">
        <property type="entry name" value="3a0107s02c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="13"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="64"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="105"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="252"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="68"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA03775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="281" mass="29827" checksum="6E0A720D0908C4A2" modified="2005-10-11" version="1">MNPIYASRRRKDIIVRALCFLAAAFGVSWLGLILFTLFYNGLAGMSWQLFIQNTPPPGASEGGLLNAVVGSLIMTVIGVGVGAPIGMFAGTYLAEYGKHDRLSPVIRFINDILLSAPSIIIGLFIYGAVVVPMGGFSALAGSLALAVIVIPVVVRTTEDMLNLVPNSLREAASALGLPRSLVIRRIAYRAARTGLITGVLLATARVAGETAPLLFTALSNQFFSLDLTSPVANLPVTINNFVQSPYVYWKQLAWSAALLITLAVLALNIGARLIGSERTAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVF8</accession>
    <name>Q3SVF8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Peptidase S24</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_0466</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase S24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ABA03733">
        <property type="protein sequence ID" value="ABA03733"/>
        <property type="gene ID" value="Nwi_0466"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0466"/>
    <dbReference type="eggNOG" id="COG2932">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107980_0_0_5"/>
    <dbReference type="OMA" id="EWIARIV"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06529">
        <property type="entry name" value="S24_LexA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039418">
        <property type="entry name" value="LexA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006197">
        <property type="entry name" value="Peptidase_S24_LexA"/>
    </dbReference>
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        <property type="entry name" value="Peptidase_S24_S26A/B/C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00717">
        <property type="entry name" value="Peptidase_S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00726">
        <property type="entry name" value="LEXASERPTASE"/>
    </dbReference>
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        <property type="entry name" value="SSF47413"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="SSF51306"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0742">SOS response</keyword>
    <feature type="domain" description="Peptidase_S24" evidence="8">
        <location>
            <begin position="87"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA03733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="210" mass="22806" checksum="2567B9F1118AF494" modified="2005-10-11" version="1">MVMLTHAQIWTALDRLAERAGLSPSGLARRAGLDPTTFNKSKRITSDGRERWPSTESVAKALAATGTAIDTFVQFIEDTERAAKAVPLLGFAEAGAGGYFDDGGFPVGKGWDEVGLPCVNDEHAYALEISGDSMKPAYRDGDVIVVSPGTPIRRGDRVVVKTRDGEVMVKELKRRTARTIELQSLNPSHANRTLPASEVEWIARIVWASQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVH4</accession>
    <name>Q3SVH4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0450</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313781.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0450"/>
    <dbReference type="EnsemblBacteria" id="ABA03717">
        <property type="protein sequence ID" value="ABA03717"/>
        <property type="gene ID" value="Nwi_0450"/>
    </dbReference>
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    <dbReference type="eggNOG" id="COG2861">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
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    <dbReference type="OMA" id="EPFDYPD"/>
    <dbReference type="OrthoDB" id="1927281at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10936">
        <property type="entry name" value="CE4_DAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006837">
        <property type="entry name" value="Divergent_DAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30105">
        <property type="entry name" value="PTHR30105"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04748">
        <property type="entry name" value="Polysacc_deac_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="400" mass="42060" checksum="09041B7EE3C2FDD8" modified="2005-10-11" version="1">MTDTTDDLSTPLGQNPAPRRRLRLPFSLPQATGLLLGSILVVFLGFVFFNHDPLGGEPKARVAIRQAPVSDDKPTAIRPPPQQPDSAAVTAPSANGQKTITIIDGSSGSRQDVVVSGPGPDAADAGSAKAVVGVDQRLLEKSRHGMIPVAAADGLKPFKAYAAGTEADRAKAAKMPVVAIVVGGLGVGATKTTDAIMKLPPAVTLAFTPYGSDPGRLVAQARAQRHEVLLQVPMEPYDYPDNDPGPQTLLTTLGAEQNIDRLHWQLSRFQGYVGLTNFMGARFVAAAAVMQPFMHEAARRGLGYLDDGAAPLSVAGRLAEGQAMPFARADLIVDAVPTSVEIDKALARLEDLAMKRGVAIGMTSALPVSVDRIGVWIKSLESRGVMLVPLTTAMLKSKST</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVQ5</accession>
    <name>Q3SVQ5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA polymerase III subunit gamma/tau</fullName>
            <ecNumber evidence="4">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">dnaX</name>
        <name evidence="7" type="ordered locus">Nwi_0369</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the POLIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the DnaX/STICHEL family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313700.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0369"/>
    <dbReference type="EnsemblBacteria" id="ABA03636">
        <property type="protein sequence ID" value="ABA03636"/>
        <property type="gene ID" value="Nwi_0369"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0369"/>
    <dbReference type="eggNOG" id="COG2812">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006229_0_7_5"/>
    <dbReference type="OMA" id="HVDVMEM"/>
    <dbReference type="OrthoDB" id="556582at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009360">
        <property type="term" value="C:DNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18137">
        <property type="entry name" value="HLD_clamp_pol_III_gamma_tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022754">
        <property type="entry name" value="DNA_pol_III_gamma-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022107">
        <property type="entry name" value="DNA_pol_III_gamma/tau_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012763">
        <property type="entry name" value="DNA_pol_III_sug/sutau_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045085">
        <property type="entry name" value="HLD_clamp_pol_III_gamma_tau"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12169">
        <property type="entry name" value="DNA_pol3_gamma3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12362">
        <property type="entry name" value="DUF3646"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="SSF48019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02397">
        <property type="entry name" value="dnaX_nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AAA" evidence="6">
        <location>
            <begin position="67"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="412"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="611" mass="65701" checksum="B32DFB098ED55AD9" modified="2005-10-11" version="1">MNDAGHPPTLPDDAAGQTGLDLGGTPSGSSVQYRVLARKYRPACFDDLIGQEALVRTVSNAFETGRIPQAWILTGVRGVGKTTTARILARALNYQLADGSVQGPTIRMPQLGVHCRAIMESRHIDVLEMDAASHTGVDDVRQINDSVRYAPSSARYKVYIIDEVHMLSTAAFNAFLKTLEEPPEHAKFVFATTEIRKVPVTVLSRCQRFDLRRVEAEVLMAHLAAIAAKENVEAEPEALGILARAAEGSVRDSLSLLDQAIAHAAGVVRADAVRRMLGLADRTRVIDLFEALARGDIASAFGEFRSQYETGADPVVVLSDLAEFVNFVTRIKIVPAIADNVAFGEAERLRASDFAVKLSIRVLSRMWQMLLKGIAEVQGATRPAAAAEMVLVRIAYVADLPTPDEAIRLLDRNGESPGVTTNAAPPRPASGPVSSVPSSAARSLSPRAAAETARPQAAAPMAGTAGAIPAVRIESLGELVALAGEKRDLIIKAALEADIRLVRIENGRLEVALERSAARTLVNDLGRKLEQWTGRRWTVIVSNEPGQPTLRSQALLQKSERERAAEADPRVQDVLARFPGAKVVEVRKFALSSPDSDAAPDELADDLDSGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVR4</accession>
    <name>Q3SVR4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313692.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0360"/>
    <dbReference type="EnsemblBacteria" id="ABA03627">
        <property type="protein sequence ID" value="ABA03627"/>
        <property type="gene ID" value="Nwi_0360"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0360"/>
    <dbReference type="eggNOG" id="COG3577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099411_3_0_5"/>
    <dbReference type="OMA" id="EFTQRGD"/>
    <dbReference type="OrthoDB" id="1803947at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05483">
        <property type="entry name" value="retropepsin_like_bacteria"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011969">
        <property type="entry name" value="Clan_AA_Asp_peptidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034122">
        <property type="entry name" value="Retropepsin-like_bacterial"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="SSF50630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02281">
        <property type="entry name" value="clan_AA_DTGA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229289" evidence="1">
        <location>
            <begin position="20"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="175" mass="18895" checksum="44A206DC0B07E64B" modified="2005-10-11" version="1">MRNLMIFAALLIGAGTYMAQLADEMTTTAAARPTTSMQPAAFETIEDHDRGGIRSVSIARDGRGHFQTEGRIDGERIGFMVDTGASVVALNESSAARVGLRPSRNDYTTTVTTANGKLKAARTRLAMVDIGGLIVRDVDAMVLPDDALSENLLGLSFLSKLRRFEFANGRMVLEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVY3</accession>
    <name>Q3SVY3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Binding-protein-dependent transport systems inner membrane component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_0291</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0291"/>
    <dbReference type="EnsemblBacteria" id="ABA03558">
        <property type="protein sequence ID" value="ABA03558"/>
        <property type="gene ID" value="Nwi_0291"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0291"/>
    <dbReference type="eggNOG" id="COG4239">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028518_1_0_5"/>
    <dbReference type="OMA" id="PSPPTWM"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025966">
        <property type="entry name" value="OppC_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12911">
        <property type="entry name" value="OppC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="236"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="307"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="354"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="188"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA03558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="390" mass="43293" checksum="02AD774775E8D3B0" modified="2005-10-11" version="1">MTSTTPVEAGATSPLGEAVPPARHSLGMSPLNRRRWQNFKANRRGYWSLWIFLILFGLSLFAELIANDRPFLIRFDGHLYFPAFVTYPETAFGGDFETAADYRDPYLQKLIADKGGTIVWPPIRYSYDTHNLDLPTPAPSKPTWLLTEAECKDVVQRKNLSGCRDLEYNWLGTDDQGRDVVARLIYGFRISVLFGLCLTIVSSVIGIAAGGVQGYFGGWVDLGFQRFIEVWTAIPSLYLLLILSSILVPGFFVLLGILLLFSWVSLVGLVRAEFLRGRNFEYITAARALGVSNATIMFRHLLPNAMVATMTFLPFIVSSSVMTLTALDFLGFGLPPGSPSLGEMLAQGKANVQAPWLGFTGFFSLAIMLSLLIFIGEAVRDAFDPRKTFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW05</accession>
    <name>Q3SW05_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Site-specific DNA-methyltransferase (adenine-specific)</fullName>
            <ecNumber evidence="3">2.1.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_0269</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:15197"/>
            <dbReference type="Rhea" id="RHEA-COMP:12418"/>
            <dbReference type="Rhea" id="RHEA-COMP:12419"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90615"/>
            <dbReference type="ChEBI" id="CHEBI:90616"/>
            <dbReference type="EC" id="2.1.1.72"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the N(4)/N(6)-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.72" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0269"/>
    <dbReference type="REBASE" id="11314">
        <property type="entry name" value="M.NwiORF269P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03536">
        <property type="protein sequence ID" value="ABA03536"/>
        <property type="gene ID" value="Nwi_0269"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0269"/>
    <dbReference type="eggNOG" id="COG0286">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018284_4_0_5"/>
    <dbReference type="OMA" id="FPYLKGF"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009007">
        <property type="term" value="F:site-specific DNA-methyltransferase (adenine-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022749">
        <property type="entry name" value="D12N6_MeTrfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003356">
        <property type="entry name" value="DNA_methylase_A-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038333">
        <property type="entry name" value="T1MK-like_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12161">
        <property type="entry name" value="HsdM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02384">
        <property type="entry name" value="N6_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HsdM_N" evidence="5">
        <location>
            <begin position="21"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="N6_Mtase" evidence="4">
        <location>
            <begin position="175"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA03536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="519" mass="58534" checksum="F27085D2A3AFF54C" modified="2005-10-11" version="1">MARTKKKNGADAAPMTTSQMLGSLLKSARDIMRKDKGLNGDLDRLPLLTWIMFLKFLDDLEQQREEETALSGKKFKAAIEAPYRWRDWAADPQGITGDELLSFINAEEAVRADGKKGPGLFAYLRALSSSNGDNRRDVIATVFKGVDNRMKSGYLLRDIINKVGGIHFTSSDELHTLGALYESMLREMRDAAGDSGEFYTPRAVVRFMVEVTDPRLGETVLDPASGTGGFLVETYNHLEKQVKTVADRKRLQDETITGCEPKSLPYLLCQMNLLLHGLDAPQIDPGNALRFKLSEIGEKERVDVILTNPPFGGEEEKGIQGNFPEDRQTAETALLFLQLIMRKLKRQPTSVGRPARAAVVVPNGTLFGDGVCARIKEELLKDFNLHTIVRLPNGVFAPYTSIPTNILFFDRSCQTKEVWYYEQPLPDGRKNYTKTQPMQFEEFKGCMAWWTKREENDQAWRVSAKELRDANCNLDRKNPRSKVDFEHLPPDQLAEDILKKELRIAELMRKIKTTLGASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW11</accession>
    <name>Q3SW11_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0263</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0263"/>
    <dbReference type="EnsemblBacteria" id="ABA03530">
        <property type="protein sequence ID" value="ABA03530"/>
        <property type="gene ID" value="Nwi_0263"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0263"/>
    <dbReference type="eggNOG" id="ENOG5033T82">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1438704_0_0_5"/>
    <dbReference type="OMA" id="HYQREAG"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="185" mass="19875" checksum="05C34D8878BD04CE" modified="2005-10-11" version="1">MSDGPHRSLPLRPHWKVLAQRAAKAAHSPAEVCEALPYALKRDILEGPLKAVRDIMGGDSLFPSMRIEQLEALRASCPGSAAATQLIDCAIVAVQNGKVGDPGAQAAVQMALEGTMHSALRSIDEHYQREAGSRSSGYVRERLDAARETVDCGALARELLTPGKPPKRRSVNLRRHTGLDEGPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW91</accession>
    <name>Q3SW91_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glutamate--cysteine ligase</fullName>
            <ecNumber evidence="2">6.3.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0182</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the synthesis of gamma-glutamylcysteine (gamma-GC).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>ATP + L-cysteine + L-glutamate = ADP + gamma-L-glutamyl-L-cysteine + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13285"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutamate--cysteine ligase type 2 family. EgtA subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.2" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313518.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0182"/>
    <dbReference type="EnsemblBacteria" id="ABA03450">
        <property type="protein sequence ID" value="ABA03450"/>
        <property type="gene ID" value="Nwi_0182"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0182"/>
    <dbReference type="eggNOG" id="COG3572">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026610_1_0_5"/>
    <dbReference type="OMA" id="DYVEWAL"/>
    <dbReference type="OrthoDB" id="991285at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004357">
        <property type="term" value="F:glutamate-cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006750">
        <property type="term" value="P:glutathione biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035434">
        <property type="entry name" value="GCL_bact_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006336">
        <property type="entry name" value="GCS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011556">
        <property type="entry name" value="Glut_cys_lig_pln_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34378">
        <property type="entry name" value="PTHR34378"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34378:SF1">
        <property type="entry name" value="PTHR34378:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04107">
        <property type="entry name" value="GCS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017901">
        <property type="entry name" value="GCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01436">
        <property type="entry name" value="glu_cys_lig_pln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 3" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR017901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="456" mass="51092" checksum="8DCC99DE5314481F" modified="2005-10-11" version="1">MARDQIDMTLLQSRDELVAWLEAGVKPASEFRIGTEHEKTPFTLGGHDPVPYEGPRGIGALLEGMRLLLGWEPIMEQGNIIGLHDVTGGGAISLEPGGQFELSGAPVETVHQTQSELAAHLAQVKEVATPLGIGFLGLGMTPSWSLAQIPMMPKGRYRIMNDYMPKVGRYGLDMMFRTCTVQTNLDFSSESDMVKKLRVSVALQPVATALFANSPFTEGKPNGFLSYRSEIWRDTDNARAGMIPWAFEDGMGFERWVDYALDVPMYFIKRGENYIDVSGSSFRDFFDGRNEAIPGERPTLSDWANHLSTIFPEVRLKRYLEMRGADGVSWGRLPALSAFWVGLLYDDASLEGAWDIAKRWNAQERQALRDDVSRLGFKARIKNRYLFEIAKECLLLAHGGLRRRGRIDRLGRDESRHLEPLDQILDSGRSPAEEMLEKFNGAWGGSVEPAYSEYAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW96</accession>
    <name>Q3SW96_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RNA polymerase sigma-54 factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Nwi_0177</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the sigma-54 factor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313514.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0177"/>
    <dbReference type="EnsemblBacteria" id="ABA03445">
        <property type="protein sequence ID" value="ABA03445"/>
        <property type="gene ID" value="Nwi_0177"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0177"/>
    <dbReference type="eggNOG" id="COG1508">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020569_0_0_5"/>
    <dbReference type="OMA" id="VTTQKFM"/>
    <dbReference type="OrthoDB" id="1906842at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001216">
        <property type="term" value="F:DNA-binding transcription activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000394">
        <property type="entry name" value="RNA_pol_sigma_54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007046">
        <property type="entry name" value="RNA_pol_sigma_54_core-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007634">
        <property type="entry name" value="RNA_pol_sigma_54_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038709">
        <property type="entry name" value="RpoN_core-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32248">
        <property type="entry name" value="PTHR32248"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00309">
        <property type="entry name" value="Sigma54_AID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04963">
        <property type="entry name" value="Sigma54_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04552">
        <property type="entry name" value="Sigma54_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000774">
        <property type="entry name" value="RpoN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00045">
        <property type="entry name" value="SIGMA54FCT"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02395">
        <property type="entry name" value="rpoN_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00717">
        <property type="entry name" value="SIGMA54_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00718">
        <property type="entry name" value="SIGMA54_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 9" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4 9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="8 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sigma54_CBD" evidence="12">
        <location>
            <begin position="156"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma54_DBD" evidence="11">
        <location>
            <begin position="358"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="49"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="512"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="512"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA03445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="543" mass="59468" checksum="72D435F700E791C8" modified="2005-10-11" version="1">MALTQKLEFRQSQSLVMTPQLMQAIKLLQLSNLDLSAYVEEELERNPLLERASDGSGPPAAEAASDVEYSEGDEAAFEDHGGGERTGAQDPASTPEEWMNRDLGSRAEIEQTFDSGLENVFPEEPAEAAARTAQDAAPSAFTEWGGGASSDDSYNLEAFVAAEITLSEHLAEQLAVALTSPAQRMIGRYLIDLVDEAGYLPADLGEAGDRLGAGESDVAAAVAVLQTFDPPGICARSLSECLAIQLREKNRYDPAMQALVEHLELLARRDFAALRRICGVDDEDLAEMIGEIRHLDPKPGLRFGAARTQSVVPDVYVRPGPDGGWLVELNSETLPRVLVNHTYYAELSKTIRKDGDKTYFTDCLQNATWLVRALDQRARTILKVATEIVRQQDGFFAHGIAHLRPLNLRTVADAIQMHESTVSRVTANKYMATNRGIFELKYFFTASIAAADGGSAHSAEAVRHHIKRLIDAESPSAILSDDTIVERLREAGIDIARRTVAKYREALRIPSSVQRRREKQNLPGGARAETRKPPNRPRDAAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWE3</accession>
    <name>Q3SWE3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Polyhydroxyalkanoate depolymerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0130</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313467.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0130"/>
    <dbReference type="ESTHER" id="nitwn-q3swe3">
        <property type="family name" value="PHB_depolymerase_PhaZ"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03398">
        <property type="protein sequence ID" value="ABA03398"/>
        <property type="gene ID" value="Nwi_0130"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0130"/>
    <dbReference type="eggNOG" id="COG4553">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017495_0_0_5"/>
    <dbReference type="OMA" id="FYLQTVE"/>
    <dbReference type="OrthoDB" id="424537at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009656">
        <property type="entry name" value="PHB_depo_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010915">
        <property type="entry name" value="PHB_depoly_PhaZ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06850">
        <property type="entry name" value="PHB_depo_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF020818">
        <property type="entry name" value="PHB_depoly_PhaZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01849">
        <property type="entry name" value="PHB_depoly_PhaZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PHB_depo_C" evidence="2">
        <location>
            <begin position="229"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="425"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="444" mass="49922" checksum="AEFB4F158A972F2F" modified="2005-10-11" version="1">MPIGEFGRPPELPAEVSPTMTAPMYWMYEMAQASLNPARAITDATRMLFQNPLNPWAHTQLGKSVAAGCELFERTTRRYGKPEWGLHETKVNGVRTPIEIRSVWEKPFCRLLYFDRMHPRPLRTPQPRLLLVAPMSGHYATLLRGTVEAFMPTHDVYITDWSDARMVPVAEGRFDLDDFVDYLIEILHMLGGNVHVIAVCQPSVPVLAAVSAMEAARDPYVPLSMTLMGGPIDTRKNPTAVNNLAAERGTDWFRNNVITKVPFPHPGVMRDVYPGFLQLNGFISMNFDRHMNAHKSLFEHLVKGDGDLADKHRDFYDEYLAVMDLTAEYYLQTVDQVFVKHALPKGEMTHRGRLVDPSKIKRVALMTVEGEKDDISGLGQTEAAHALCTSIPDNRRTHYVQSGVGHYGVFNGSRFRSEIVPRMNDFMQSNAGPPEKASRASAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="126" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWF6</accession>
    <name>Q3SWF6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glutathione synthetase</fullName>
            <ecNumber evidence="10">6.3.2.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">GSH synthetase</fullName>
            <shortName evidence="10">GSH-S</shortName>
            <shortName evidence="10">GSHase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Glutathione synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">gshB</name>
        <name evidence="13" type="ordered locus">Nwi_0117</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + glutathione + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13557"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the prokaryotic GSH synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.3" evidence="10"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313454.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0117"/>
    <dbReference type="EnsemblBacteria" id="ABA03385">
        <property type="protein sequence ID" value="ABA03385"/>
        <property type="gene ID" value="Nwi_0117"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0117"/>
    <dbReference type="eggNOG" id="COG0189">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068239_0_0_5"/>
    <dbReference type="OMA" id="WMRKDPP"/>
    <dbReference type="OrthoDB" id="878336at2"/>
    <dbReference type="UniPathway" id="UPA00142">
        <property type="reaction ID" value="UER00210"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004363">
        <property type="term" value="F:glutathione synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00162">
        <property type="entry name" value="GSH_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006284">
        <property type="entry name" value="Glut_synth_pro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004218">
        <property type="entry name" value="GSHS_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004215">
        <property type="entry name" value="GSHS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621:SF4">
        <property type="entry name" value="PTHR21621:SF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02955">
        <property type="entry name" value="GSH-S_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02951">
        <property type="entry name" value="GSH-S_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01380">
        <property type="entry name" value="glut_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="3 10 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="12">
        <location>
            <begin position="125"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA03385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="317" mass="34980" checksum="9098C5F8AECA97D2" modified="2005-10-11" version="1">MKLKVAVQMDPIARINIRGDSTFALLLEAQRRGHDVSYYTPDKLSLRGRDVVAPVQPLTVRDHHGDHFTLGDATRAPLESFDVVLLRQDPPFDLAYITTTHLLERIHPKTLVVNNPASVRNAPEKLFVMDFPDLMPPTLISRDLDEINSFRAEHGAVVMKPLHGHGGAAVFRVMPQDMNFGSLFDMFSVTFRESWVIQRFLPEVSRGDKRIILVDGEFAGAVNRVPAADDLRSNMVRGGAARATDLTPRESEICERIGPALRERGLLFVGIDVIDGLLTEINVTSPTGIRAIAALGGPDVAAIVWDRIEAKRSQAPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWH9</accession>
    <name>Q3SWH9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Chromosome segregation DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_0094</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in chromosome partition. Localize to both poles of the predivisional cell following completion of DNA replication. Binds to the DNA origin of replication.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ParB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313431.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0094"/>
    <dbReference type="EnsemblBacteria" id="ABA03362">
        <property type="protein sequence ID" value="ABA03362"/>
        <property type="gene ID" value="Nwi_0094"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0094"/>
    <dbReference type="eggNOG" id="COG1475">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023853_0_0_5"/>
    <dbReference type="OMA" id="IALSYQR"/>
    <dbReference type="OrthoDB" id="653368at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041468">
        <property type="entry name" value="HTH_ParB/Spo0J"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004437">
        <property type="entry name" value="ParB/RepB/Spo0J"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB/Sulfiredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17762">
        <property type="entry name" value="HTH_ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02195">
        <property type="entry name" value="ParBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00470">
        <property type="entry name" value="ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="SSF110849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00180">
        <property type="entry name" value="parB_part"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ParB" evidence="5">
        <location>
            <begin position="34"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="223"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA03362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="293" mass="32102" checksum="C565738C3C2EC083" modified="2005-10-11" version="1">MADEARSRLGRGLASLIGDVGGEAAHAERPRVQRKVPIEFLSPNPRNPRRTFSDVELAELSDSIKQHGVIQPIVVRPVKGMQDRFEIIAGERRWRAAQSAGLHEVPIVPVDVSDHVALEIAIVENVQREDLNAMEEAQGYHALANEFNRTQDDIAKIVGKSRSHVANMMRLTKLPDDVRALIASGELSAGHARALIGMPDPSTAAKRIIAEGLNVRQAEALAHEDGAAERKPQKARADKARKDSDTLALEKRVSDALGLAVTVDHKDPGGVVQIRYRDLDQLDEILKRLDPKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-21" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWN3</accession>
    <name>FPG_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Formamidopyrimidine-DNA glycosylase</fullName>
            <shortName evidence="2">Fapy-DNA glycosylase</shortName>
            <ecNumber evidence="2">3.2.2.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">DNA-(apurinic or apyrimidinic site) lyase MutM</fullName>
            <shortName evidence="2">AP lyase MutM</shortName>
            <ecNumber evidence="2">4.2.99.18</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">mutM</name>
        <name evidence="2" type="synonym">fpg</name>
        <name type="ordered locus">Nwi_0040</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine.</text>
            <dbReference type="EC" id="3.2.2.23"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-monophospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="2">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FPG family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.23" evidence="2"/>
    <dbReference type="EC" id="4.2.99.18" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313379.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWN3"/>
    <dbReference type="SMR" id="Q3SWN3"/>
    <dbReference type="STRING" id="323098.Nwi_0040"/>
    <dbReference type="EnsemblBacteria" id="ABA03308">
        <property type="protein sequence ID" value="ABA03308"/>
        <property type="gene ID" value="Nwi_0040"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0040"/>
    <dbReference type="eggNOG" id="COG0266">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038423_1_1_5"/>
    <dbReference type="OMA" id="GVHLRMT"/>
    <dbReference type="OrthoDB" id="1162346at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008534">
        <property type="term" value="F:oxidized purine nucleobase lesion DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00103">
        <property type="entry name" value="Fapy_DNA_glycosyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015886">
        <property type="entry name" value="DNA_glyclase/AP_lyase_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015887">
        <property type="entry name" value="DNA_glyclase_Znf_dom_DNA_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020629">
        <property type="entry name" value="Formamido-pyr_DNA_Glyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012319">
        <property type="entry name" value="FPG_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035937">
        <property type="entry name" value="MutM-like_N-ter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_S13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000214">
        <property type="entry name" value="Znf_DNA_glyclase/AP_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010663">
        <property type="entry name" value="Znf_FPG/IleRS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01149">
        <property type="entry name" value="Fapy_DNA_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06831">
        <property type="entry name" value="H2TH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06827">
        <property type="entry name" value="zf-FPG_IleRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00898">
        <property type="entry name" value="Fapy_DNA_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01232">
        <property type="entry name" value="H2TH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="SSF46946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81624">
        <property type="entry name" value="SSF81624"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00577">
        <property type="entry name" value="fpg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51068">
        <property type="entry name" value="FPG_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01242">
        <property type="entry name" value="ZF_FPG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51066">
        <property type="entry name" value="ZF_FPG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0326">Glycosidase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="initiator methionine" description="Removed" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000228451" description="Formamidopyrimidine-DNA glycosylase">
        <location>
            <begin position="2"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="FPG-type" evidence="2">
        <location>
            <begin position="257"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with DNA" evidence="2">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="3"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for beta-elimination activity" evidence="2">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for delta-elimination activity" evidence="2">
        <location>
            <position position="283"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>DNA</name>
            <dbReference type="ChEBI" id="CHEBI:16991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>DNA</name>
            <dbReference type="ChEBI" id="CHEBI:16991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>DNA</name>
            <dbReference type="ChEBI" id="CHEBI:16991"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00103"/>
        </source>
    </evidence>
    <sequence length="293" mass="32337" checksum="460D348F3071BE27" modified="2007-01-23" version="3">MPELPEVETVRRGLQPAMEGATIVRAETRRKDLRFPFQTDFVARLEGQAVTGLGRRAKYLLADLASGDVLLMHLGMSGSFRVIDAAGVTVPGDFHRPRGEDRVHDHVRFTMSSRAEIVFNDPRRFGYMKIVARSALGDEPLLKGLGPEPLGNEFDAAVLAHGCRNRKTSLKAALLDQRVVAGLGNIYVCEALFRARLSPRRLAATLAMKTGAPSERAERLVGAIRDVLNQAIEAGGSSLRDHRQTTGELGYFQHSFQVYDREGDKCRTPACKGAVKRFTQNGRSTFWCPVCQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWQ8</accession>
    <name>Q3SWQ8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">PhoH-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_0015</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PhoH family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313354.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0015"/>
    <dbReference type="EnsemblBacteria" id="ABA03283">
        <property type="protein sequence ID" value="ABA03283"/>
        <property type="gene ID" value="Nwi_0015"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0015"/>
    <dbReference type="eggNOG" id="COG1702">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051654_0_0_5"/>
    <dbReference type="OMA" id="NLCGQFD"/>
    <dbReference type="OrthoDB" id="445854at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003714">
        <property type="entry name" value="PhoH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02562">
        <property type="entry name" value="PhoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="SSF54791"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PhoH" evidence="5">
        <location>
            <begin position="135"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA03283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="349" mass="37922" checksum="B3C8C6FB364A42B8" modified="2005-10-11" version="1">MPKSASDSPPLAPSRKPEIQGPPETQVVIDFEDNRAVSALVGPYGQNLSQLERRLGVVVDSRGNHITIAGSRDGCDAGKRVLETLYAQAAQGHDLSQGEVEGAIRAAIAQGSLFEDDVKAPRSGFEAINLRKRPVRARTAAQDLYIRALKRHELVFGIGPAGTGKTWLAVAQATQLFERKEVDRIILTRPAVEAGERLGFLPGDLREKVDPYLRPIYDALYDLMDSRIVERALQSGEIEIAPLAFMRGRTLTNAAIILDEAQNTTSMQMKMFLTRLGENSRMIVTGDPSQVDLPNGQTSGLTEATRLLDGVEGVAQVTFIAEDVIRHELVARIVAAYEGLPPRPAAGKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMX8</accession>
    <name>Q3SMX8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase, IS4 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_3116</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06363">
        <property type="protein sequence ID" value="ABA06363"/>
        <property type="gene ID" value="Nwi_3116"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3116"/>
    <dbReference type="eggNOG" id="COG3385">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055261_9_1_5"/>
    <dbReference type="OMA" id="FGRIKDW"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE_Tnp_1" evidence="1">
        <location>
            <begin position="4"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="157" mass="17413" checksum="8981DD6BC2A51742" modified="2005-10-11" version="1">MLADALGRPLRFIVTAGQVGDITQAPALLEAQAGDAVLADKAYDSNALRALIAGMGAEAVIPSNRTRKIIIPHDAGLYKHRNRIERCFNRLKHFRRFATRYDRRTVHFTGFVHLAAALIWLPRCGSELLQDHRIGPMTCSQDAWLIAASLPSHCQTD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN24</accession>
    <name>DAPD_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase</fullName>
            <ecNumber evidence="1">2.3.1.117</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Tetrahydrodipicolinate N-succinyltransferase</fullName>
            <shortName evidence="1">THDP succinyltransferase</shortName>
            <shortName evidence="1">THP succinyltransferase</shortName>
            <shortName evidence="1">Tetrahydropicolinate succinylase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dapD</name>
        <name type="ordered locus">Nwi_3069</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-2,3,4,5-tetrahydrodipicolinate + H2O + succinyl-CoA = (S)-2-succinylamino-6-oxoheptanedioate + CoA</text>
            <dbReference type="Rhea" id="RHEA:17325"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15685"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="EC" id="2.3.1.117"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route): step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.117" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316233.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SN24"/>
    <dbReference type="SMR" id="Q3SN24"/>
    <dbReference type="STRING" id="323098.Nwi_3069"/>
    <dbReference type="EnsemblBacteria" id="ABA06317">
        <property type="protein sequence ID" value="ABA06317"/>
        <property type="gene ID" value="Nwi_3069"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3069"/>
    <dbReference type="eggNOG" id="COG2171">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050859_0_1_5"/>
    <dbReference type="OMA" id="YFPIQKM"/>
    <dbReference type="OrthoDB" id="752123at2"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00019"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008666">
        <property type="term" value="F:2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.166.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00811">
        <property type="entry name" value="DapD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005664">
        <property type="entry name" value="DapD_Trfase_Hexpep_rpt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023180">
        <property type="entry name" value="THP_succinylTrfase_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037133">
        <property type="entry name" value="THP_succinylTrfase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14602">
        <property type="entry name" value="Hexapep_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14805">
        <property type="entry name" value="THDPS_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00965">
        <property type="entry name" value="dapD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword id="KW-0457">Lysine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000047158" description="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase">
        <location>
            <begin position="1"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00811"/>
        </source>
    </evidence>
    <sequence length="281" mass="29938" checksum="2A30E8590CBE9459" modified="2005-10-11" version="1">MPTASLETTINGAFDARETITPATRGEVRDAVDQALGLLDKGEARVAERAADGKWQVNQWLKKAVLLSFRLNDMSVIPGGPGNAAWWDKVPSKFEGWDENRFRDAGFRAVPGAIVRRSAFIARNVVLMPSFVNLGAYVDESTMVDTWCTVGSCAQIGKRVHISGGAGIGGVLEPLQAGPVIIEDDCFIGARSEVAEGVIVRKGAVLSMGVFLGASTKIVDRTTGEIFMGEVPEYAVVVPGALPGKPLSNGQLGPSTACAVIVKRVDERTRSKTGINELLRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN50</accession>
    <name>Q3SN50_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_3041</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06291">
        <property type="protein sequence ID" value="ABA06291"/>
        <property type="gene ID" value="Nwi_3041"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3041"/>
    <dbReference type="HOGENOM" id="CLU_2494760_0_0_5"/>
    <dbReference type="OMA" id="FRRCDIS"/>
    <dbReference type="OrthoDB" id="2148334at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="86" mass="9872" checksum="2C4F66A3248AE65D" modified="2005-10-11" version="1">MMTDADKVEFGNRLAKLVADKHWTDVEFAREASKRFRGPHDFRRCDISLYMRGIRFPDPSRLKAICETLGVEEKALKGDAHVGVKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN71</accession>
    <name>Q3SN71_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_3020</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPF0283 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316188.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3020"/>
    <dbReference type="EnsemblBacteria" id="ABA06270">
        <property type="protein sequence ID" value="ABA06270"/>
        <property type="gene ID" value="Nwi_3020"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3020"/>
    <dbReference type="eggNOG" id="COG3768">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057693_1_0_5"/>
    <dbReference type="OMA" id="MFFIAWR"/>
    <dbReference type="OrthoDB" id="1529001at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021147">
        <property type="entry name" value="DUF697"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006507">
        <property type="entry name" value="UPF0283"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39342">
        <property type="entry name" value="PTHR39342"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05128">
        <property type="entry name" value="DUF697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01620">
        <property type="entry name" value="hyp_HI0043"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="26"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA06270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="348" mass="37253" checksum="9CC112E2E61145DE" modified="2005-10-11" version="1">MSERTGQRRPVTFKLDDPHVVVLERDEAAARPARGSISITPEAEPEPEPVLLPESPDTPPVPARKGFRWGVLFWSSLAGLILLGTVLGAINLIGDLFARSEGLGFLGLTLAALFVVASIVIAAREILGLARLATIENLHRRALAAIAADDRKEADAVVRDLVKLAHNDPHLARARATLRSHSTDIIDGADLVRLAERELMAPLDQEARRLVSVAAQRVSIVTAISPRAVVDVLFVFAAAMRLVRQLARLYGGRPGTLGMIRLMRHVIAHLAITGGIAASDTLIQQMVGQGIAAKLSTRLGEGVLNGLLTARLGLAAIDVTRPMPFTALPRPALGDLVKDLLRKRDGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNL5</accession>
    <name>Q3SNL5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2876</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316058.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2876"/>
    <dbReference type="EnsemblBacteria" id="ABA06126">
        <property type="protein sequence ID" value="ABA06126"/>
        <property type="gene ID" value="Nwi_2876"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2876"/>
    <dbReference type="HOGENOM" id="CLU_1254841_0_0_5"/>
    <dbReference type="OMA" id="YLETEWT"/>
    <dbReference type="OrthoDB" id="2130017at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="220" mass="23631" checksum="E148FA70D3714904" modified="2005-10-11" version="1">MIKVPFSSLFDAFRMPFVNRRIVKKLESLDAQESFSDIKSLSEEDIEKYIESEWTRAKELDDKLSKLTAALSVALTVGGAVAKSIVDGLAASPIKWTIVALLFLSMVFFFYGAIIGFRGLRPKPRFGYGAKFMNAVASGGESARKSMNEAAAGFEVVNLIRANEASAAIDLIRNGIVLFAIAMALSFVAPGIEVSPRTATISTKGSAPAISKKQKAANGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNL9</accession>
    <name>Q3SNL9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2872</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316054.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2872"/>
    <dbReference type="EnsemblBacteria" id="ABA06122">
        <property type="protein sequence ID" value="ABA06122"/>
        <property type="gene ID" value="Nwi_2872"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2872"/>
    <dbReference type="eggNOG" id="COG0476">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1239094_0_0_5"/>
    <dbReference type="OrthoDB" id="555145at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032865">
        <property type="entry name" value="Prok-E2_A"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14457">
        <property type="entry name" value="Prok-E2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="223" mass="24876" checksum="50B6640B77C88DDC" modified="2005-10-11" version="1">MEPGMAGNDYRGHAERFLAAALRHPECRGGRLISVDAGGSRIELDLNVEMPLAFKVDGASPNGVRVVETVNVRLWPSYPWSSPSFYLRMDFPRDLPHVQPGPVTEPPRPCLIDGNQREYFFQFGLVELGIFNLVHQLVLWLQRAAEGTLIHHGRGWEPTLRCDLNDVIALNAEACRAVVDRNGGYRVLKSTFFRSGGDGSLVDKQGTTWLDVIRGCGLHARDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNR3</accession>
    <name>Q3SNR3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2825</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2825"/>
    <dbReference type="EnsemblBacteria" id="ABA06078">
        <property type="protein sequence ID" value="ABA06078"/>
        <property type="gene ID" value="Nwi_2825"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2825"/>
    <dbReference type="eggNOG" id="ENOG502ZBQE">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119921_0_0_5"/>
    <dbReference type="OMA" id="EAFQTHY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="90" mass="9439" checksum="3FC2ABCC88BEF170" modified="2005-10-11" version="1">MIRLDLSSEPKWLDLGAGLRLHVLPVTTAIMVAARNDPAVEALPEGASKEEQALVMAKAVARRVVTGWEGVGDADGDPVPVTPEGIDALL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNV7</accession>
    <name>RIMM_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosome maturation factor RimM</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rimM</name>
        <name type="ordered locus">Nwi_2781</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">An accessory protein needed during the final step in the assembly of 30S ribosomal subunit, possibly for assembly of the head region. Probably interacts with S19. Essential for efficient processing of 16S rRNA. May be needed both before and after RbfA during the maturation of 16S rRNA. It has affinity for free ribosomal 30S subunits but not for 70S ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Binds ribosomal protein S19.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The PRC barrel domain binds ribosomal protein S19.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RimM family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315979.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SNV7"/>
    <dbReference type="SMR" id="Q3SNV7"/>
    <dbReference type="STRING" id="323098.Nwi_2781"/>
    <dbReference type="EnsemblBacteria" id="ABA06034">
        <property type="protein sequence ID" value="ABA06034"/>
        <property type="gene ID" value="Nwi_2781"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2781"/>
    <dbReference type="eggNOG" id="COG0806">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077636_0_1_5"/>
    <dbReference type="OMA" id="DEFYHAD"/>
    <dbReference type="OrthoDB" id="1497415at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00014">
        <property type="entry name" value="Ribosome_mat_RimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011961">
        <property type="entry name" value="16S_RimM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002676">
        <property type="entry name" value="RimM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036976">
        <property type="entry name" value="RimM_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01782">
        <property type="entry name" value="RimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="SSF50346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02273">
        <property type="entry name" value="16S_RimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="chain" id="PRO_0000244140" description="Ribosome maturation factor RimM">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="PRC barrel" evidence="1">
        <location>
            <begin position="92"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00014"/>
        </source>
    </evidence>
    <sequence length="173" mass="18277" checksum="56B800F3B2E7F8EC" modified="2005-10-11" version="1">MTARVCVARIGAAHGLRGEVRLWTFTEDPMSVTRYGPLSTKDGARQLEVSHARAASDHLIATLTGVTTREEAERLNGTELYVARDKLPETDEGEFYHADLIGLAAFDAAGTALGTVAAIHNFGAGDIIEIAPARGPTLMLPFTDAVVPIVDIAGGRVVIEAPAEIDGEEPNSV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNZ1</accession>
    <name>RL31_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpmE</name>
        <name type="ordered locus">Nwi_2747</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds the 23S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL31 family. Type A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315945.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SNZ1"/>
    <dbReference type="STRING" id="323098.Nwi_2747"/>
    <dbReference type="EnsemblBacteria" id="ABA06000">
        <property type="protein sequence ID" value="ABA06000"/>
        <property type="gene ID" value="Nwi_2747"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2747"/>
    <dbReference type="eggNOG" id="COG0254">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114306_3_2_5"/>
    <dbReference type="OMA" id="YHEITVV"/>
    <dbReference type="OrthoDB" id="2014569at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00501">
        <property type="entry name" value="Ribosomal_L31_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034704">
        <property type="entry name" value="L28p-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002150">
        <property type="entry name" value="Ribosomal_L31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027491">
        <property type="entry name" value="Ribosomal_L31_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042105">
        <property type="entry name" value="Ribosomal_L31_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33280">
        <property type="entry name" value="PTHR33280"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01197">
        <property type="entry name" value="Ribosomal_L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01249">
        <property type="entry name" value="RIBOSOMALL31"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143800">
        <property type="entry name" value="SSF143800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00105">
        <property type="entry name" value="L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01143">
        <property type="entry name" value="RIBOSOMAL_L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000259200" description="50S ribosomal protein L31">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00501"/>
        </source>
    </evidence>
    <sequence length="75" mass="8459" checksum="D7E61571953C9EE6" modified="2005-10-11" version="1">MKAEIHPDYHTIKVVMTDGTEYLTRSTWGKDGDTMNLDIDSKSHPAWTGGSQQILDRGGRVSRFQKKFSGFLKKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP10</accession>
    <name>Q3SP10_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Magnesium transporter MgtE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_2728</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Acts as a magnesium transporter.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SLC41A transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315927.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2728"/>
    <dbReference type="EnsemblBacteria" id="ABA05981">
        <property type="protein sequence ID" value="ABA05981"/>
        <property type="gene ID" value="Nwi_2728"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2728"/>
    <dbReference type="eggNOG" id="COG2239">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037408_2_2_5"/>
    <dbReference type="OMA" id="TMTVAVR"/>
    <dbReference type="OrthoDB" id="1239195at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.60.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006668">
        <property type="entry name" value="Mg_transptr_MgtE_intracell_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038076">
        <property type="entry name" value="MgtE_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006669">
        <property type="entry name" value="MgtE_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006667">
        <property type="entry name" value="SLC41_membr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036739">
        <property type="entry name" value="SLC41_membr_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43773">
        <property type="entry name" value="PTHR43773"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01769">
        <property type="entry name" value="MgtE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03448">
        <property type="entry name" value="MgtE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00924">
        <property type="entry name" value="MgtE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161093">
        <property type="entry name" value="SSF161093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00400">
        <property type="entry name" value="mgtE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0129">CBS domain</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="349"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="386"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="428"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="8">
        <location>
            <begin position="132"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="8">
        <location>
            <begin position="196"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="456" mass="49100" checksum="7387A8D8D21DF1F8" modified="2005-10-11" version="1">MTDTHTATTDGDLDVFALASRLSGGHVADNVEILNQLEPQDAAAVLVLLPVDISIEILDKPELNFGPEIVVALPRQTATILLAGMSADMAADIVQQLEEPARTELMQALDEPTRTTIEGLLAYPENTAGAMMTTEFVTVPANWTAERTLQHIRQVERTRETVYAIYVLDAFTRRLVGAVSLRRLISGEPSARIAELARSPITTSPLTDREEVARLISRHDMLAVPVVDKAGHVLGIVTVDDIIDAIIEENTEDAQKFGGMAATDQPYLQIGLPGMIRKRGGWLCVLFLGELLTASAMQHFEGELEKAIVLTLFIPLIMSSGGNSGSQATSLLIRALALREVKLGDWWRVALLELPTGLILGAMLGVIGLVRVVAWQKFGIYDYGEHWMLIGLTVAAALIGVVVFGSVAGSMLPFMLQRAGFDPASASAPLVATLVDVTGLMIYFSVALLILSGTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP56</accession>
    <name>Q3SP56_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Peptidase S11, D-alanyl-D-alanine carboxypeptidase 1</fullName>
            <ecNumber evidence="12">3.4.16.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_2682</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the peptidase S11 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.4" evidence="12"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2682"/>
    <dbReference type="EnsemblBacteria" id="ABA05935">
        <property type="protein sequence ID" value="ABA05935"/>
        <property type="gene ID" value="Nwi_2682"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2682"/>
    <dbReference type="eggNOG" id="COG1686">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027070_1_4_5"/>
    <dbReference type="OMA" id="PWYPASI"/>
    <dbReference type="OrthoDB" id="1499212at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009002">
        <property type="term" value="F:serine-type D-Ala-D-Ala carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018044">
        <property type="entry name" value="Peptidase_S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001967">
        <property type="entry name" value="Peptidase_S11_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00768">
        <property type="entry name" value="Peptidase_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00725">
        <property type="entry name" value="DADACBPTASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="3" id="KW-0133">Cell shape</keyword>
    <keyword evidence="5" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="12" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229141" evidence="10">
        <location>
            <begin position="35"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S11" evidence="11">
        <location>
            <begin position="31"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="297"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="373"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="302"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="382"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="415"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="6">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="122"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR618044-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR618044-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA05935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="455" mass="48014" checksum="515B4ACF69F2D723" modified="2005-10-11" version="1">MHSSIFPFRKWLPTCIVLAAVALVTLAPSRTVHATEAHLLIEADTGKVLEAENATFPWYPASVTKLMTAYVTLTAVKQKRLTLDTLLTVSPTAAAQSPSKMGFRPGTQVTVDNALKMMMVKSANDMAVVLAEGVGGSIDGFSAEMNAAAQRLGMTQSSYVNPNGLPADGQISSARDMAILARAFIHDLPEYEYFVHIPSIRYGRRVTHNFNKLIGRFPGADGFKTGFICASGYNLVASATQNGKRLIAVVLGASSGTMRAVRAAQMLERGFANNSLSWLRPALGTVDRLAPIAATPPNLRDEMCGPKRRHPASDEDNDVVANTSATSAAVMSFFATGAQPPTLRPVDMIAAAPAPSDPVIVYTGPTRTGAAIAAAAEADAARDAPPKSKKPGKTARDKPAPKAEAREKSKNRVSITIDPKSASKSTPKSTKPPVNTKPAASPSAKPKTAEGKSKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP60</accession>
    <name>Q3SP60_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2678</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315877.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2678"/>
    <dbReference type="EnsemblBacteria" id="ABA05931">
        <property type="protein sequence ID" value="ABA05931"/>
        <property type="gene ID" value="Nwi_2678"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2678"/>
    <dbReference type="HOGENOM" id="CLU_2899578_0_0_5"/>
    <dbReference type="OMA" id="RTMAFKR"/>
    <dbReference type="OrthoDB" id="2162585at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="62" mass="7307" checksum="82B17237AB217288" modified="2005-10-11" version="1">MMTINDDLEKFRQARTMAFKRGFVVRRADDPDDPKPYTLVRQAVETHLATIDEVLEELKRTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP70</accession>
    <name>Q3SP70_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CrtC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2668</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315869.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2668"/>
    <dbReference type="EnsemblBacteria" id="ABA05921">
        <property type="protein sequence ID" value="ABA05921"/>
        <property type="gene ID" value="Nwi_2668"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2668"/>
    <dbReference type="eggNOG" id="COG5621">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040626_0_0_5"/>
    <dbReference type="OMA" id="AWLDHEW"/>
    <dbReference type="OrthoDB" id="591394at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.370.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023374">
        <property type="entry name" value="AttH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010791">
        <property type="entry name" value="AttH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07143">
        <property type="entry name" value="CrtC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CrtC" evidence="1">
        <location>
            <begin position="64"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="362" mass="39862" checksum="CEB77DF3E635718C" modified="2005-10-11" version="1">MSASPKISRRAFATGLAFLGFGRGASLAQNFAGLGGDASGFASVMPGKSFEFPRDHGPHPDYRIEWWYLTANLRDHAGDALGIQWTLFRQAIQPGPQREGWANQHVWMGHAAVTRADAHRCGETFARGGVGQAGVDADPFHAWIDSWRMRGLDRVSAMNVAPLEVNASSAEFSYVLRLEADKALVLQGDAGHSVKSERGQASYYYSQPHFTAAGTVTIDGRSADVIGQAWMDREWSSQPLAPDQTGWDWFSLHLETGEKLMLYRLRQTRGEPYLTGNWIGSDGRSAALSPRTADMIPTATTEVAGRRMPTGWRIIIQDRGMTIDTVPLNPASWMGTRFSYWEGPVSFTGTHRGVGYLEMTGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP85</accession>
    <name>Q3SP85_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transcriptional regulator, BadM/Rrf2 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2653</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315856.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2653"/>
    <dbReference type="EnsemblBacteria" id="ABA05906">
        <property type="protein sequence ID" value="ABA05906"/>
        <property type="gene ID" value="Nwi_2653"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2653"/>
    <dbReference type="eggNOG" id="COG1959">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107144_2_1_5"/>
    <dbReference type="OMA" id="SCISLNW"/>
    <dbReference type="OrthoDB" id="1765806at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000944">
        <property type="entry name" value="Tscrpt_reg_Rrf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33221">
        <property type="entry name" value="PTHR33221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02082">
        <property type="entry name" value="Rrf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00738">
        <property type="entry name" value="rrf2_super"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51197">
        <property type="entry name" value="HTH_RRF2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="142" mass="15743" checksum="AB9F7A89E98343E1" modified="2005-10-11" version="1">MRLTSFFDYTLRLLMYAATHGDRLITIEETASVYGISRTHLMKVANQLTRAGYLKAVRGRSGGLMLAKRPNRIKLGDVLCATEPDFALVECFSSNNRCPITSSCRLRAPLREALNAFTTTLDRYTLADLILSPKDFGVPPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPB0</accession>
    <name>Q3SPB0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Double-transmembrane region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2628</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2628"/>
    <dbReference type="EnsemblBacteria" id="ABA05881">
        <property type="protein sequence ID" value="ABA05881"/>
        <property type="gene ID" value="Nwi_2628"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2628"/>
    <dbReference type="eggNOG" id="ENOG502Z7KE">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014519_0_0_5"/>
    <dbReference type="OMA" id="TWSNLPL"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024163">
        <property type="entry name" value="Aerotolerance_reg_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011933">
        <property type="entry name" value="Double_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025297">
        <property type="entry name" value="DUF4159"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07584">
        <property type="entry name" value="BatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13709">
        <property type="entry name" value="DUF4159"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02226">
        <property type="entry name" value="two_anch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="626"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="657"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="domain" description="BatA" evidence="2">
        <location>
            <begin position="7"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4159" evidence="3">
        <location>
            <begin position="696"/>
            <end position="917"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="937" mass="100046" checksum="3AC7DC0B09CF3E7E" modified="2005-10-11" version="1">MMPALPLSFLQPLMLLGLLSLPLLWWLLRVMPPRPRRIDFPPTRLLFDIAPKEETPSRTPWWLTALRLAAAALVILAAAGPVWNPKTGAARGAAPLTILLDDGWSAASSWETRIKAADELITEADGDRRGVAIVPLSEPARDITLMPAGTARVALRQLVPKPFAVERVATLPALERFLKATGDSDIAWLSDGIDSGRGVEFVDGLRKVIGDRSLTVVEGGAPPARALTAAENAAARMTVKVLRAAANGSDSGMVRALDQKGAPIGEAPYAFALQDRETEAAFDLPLELRNDIARLEIAGERSAGAVQLLDKRWRRRAIGVVSGSSAERAQPLLAPTFYLSRALAPFADVRLDDKSAPQQAITQFLDQKLPMIVMADVGALSPELRHRIDDWIGRGGVLVRFAGPRLAQADDGLVPVKLRRGGRSLGGSLTWEKPQHLAALAEDGPFAGLPVPNDVTVSRQVLAEPDATLAAKSWASLVDGTPLVTGERRGKGLLVLFHISADMRWSDLPMSGTFVEMLRRIVDASGYTANIGRIDTNTRTETAAPLRTLDGFGVLTPPPATVKPLPVDDRGRGTIEHPPGFYGPVDAPIAVNTLAATDRIVPLDTSNLTARRASFTNAEPHDLRGVLLSSALALFLADAVIVALLGGGLAMLLRRRAAHAALAVALALPAIAIAPSPSRADAASDTFAMKAVAQTRLAYVVTGDAGVDAIVKAGMSGLTLFLAQRTALEAGEPVGVDPARDELAFFPLIYWPVVPGAPKPPQKALDRIDAYMKQGGTILFDTRDAIDALPGTDGESQTPGMQQLRIILASLDIPELEPVPHEHVLAKTFYLLHGFPGRFTTGQTWVEALPRSEDDEAASRPARGGDGVSPIIITSNDLAGAWAMRPDGQPMLPLSPGKPRQREFAFRAGVNIVMYTLTGNYKADQVHAPALIERLGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPD9</accession>
    <name>Q3SPD9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Small multidrug resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_2599</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the drug/metabolite transporter (DMT) superfamily. Small multidrug resistance (SMR) (TC 2.A.7.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315802.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2599"/>
    <dbReference type="EnsemblBacteria" id="ABA05852">
        <property type="protein sequence ID" value="ABA05852"/>
        <property type="gene ID" value="Nwi_2599"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2599"/>
    <dbReference type="eggNOG" id="COG2076">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133067_0_2_5"/>
    <dbReference type="OMA" id="EGFTQAW"/>
    <dbReference type="OrthoDB" id="1921902at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042221">
        <property type="term" value="P:response to chemical"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000390">
        <property type="entry name" value="Small_drug/metabolite_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045324">
        <property type="entry name" value="Small_multidrug_res"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30561">
        <property type="entry name" value="PTHR30561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00893">
        <property type="entry name" value="Multi_Drug_Res"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="109" mass="11296" checksum="32AA40524E0BFC0F" modified="2005-10-11" version="1">MNAWGLLAISIVAEVTATTLLTKSEGFGKPVYGVAALAIFAGCFWALSQVLTRIPVGVAYAIWSGAGVVLISLIGAIFLRQSPSMIQILFISLIVVGAVGLNLTTSVSQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPH2</accession>
    <name>HIS1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP phosphoribosyltransferase</fullName>
            <shortName evidence="1">ATP-PRT</shortName>
            <shortName evidence="1">ATP-PRTase</shortName>
            <ecNumber evidence="1">2.4.2.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisG</name>
        <name type="ordered locus">Nwi_2566</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of ATP and 5-phosphoribose 1-diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + ATP</text>
            <dbReference type="Rhea" id="RHEA:18473"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="2.4.2.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Feedback inhibited by histidine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP phosphoribosyltransferase family. Long subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.17" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315770.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SPH2"/>
    <dbReference type="SMR" id="Q3SPH2"/>
    <dbReference type="STRING" id="323098.Nwi_2566"/>
    <dbReference type="EnsemblBacteria" id="ABA05819">
        <property type="protein sequence ID" value="ABA05819"/>
        <property type="gene ID" value="Nwi_2566"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2566"/>
    <dbReference type="eggNOG" id="COG0040">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038115_0_1_5"/>
    <dbReference type="OMA" id="LDIHNTR"/>
    <dbReference type="OrthoDB" id="1419568at2"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003879">
        <property type="term" value="F:ATP phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00079">
        <property type="entry name" value="HisG_Long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013820">
        <property type="entry name" value="ATP_PRibTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018198">
        <property type="entry name" value="ATP_PRibTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001348">
        <property type="entry name" value="ATP_PRibTrfase_HisG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020621">
        <property type="entry name" value="ATP_PRibTrfase_HisG_long"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21403">
        <property type="entry name" value="PTHR21403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01634">
        <property type="entry name" value="HisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00070">
        <property type="entry name" value="hisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01316">
        <property type="entry name" value="ATP_P_PHORIBOSYLTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000004482" description="ATP phosphoribosyltransferase">
        <location>
            <begin position="1"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00079"/>
        </source>
    </evidence>
    <sequence length="325" mass="35145" checksum="7F6DA1E37D140B82" modified="2005-10-11" version="1">MTTSLVLAVPSKGRLQENAEAFFARAGLTLAKPRGVRDYRGTIAELDNVEIAYLSASEIASQLARGTVHLGVTGEDLIRESIVDADKRVALITSLGFGNANVVVAVPQSWIDVRTMADLDDVATGFRAQHNRRMRVATKYINLTRAFFASHGVVDYRIVESAGATEGAPAVGTAEMIVDITTTGATLTANGLRVLDDGVILRSQANLVASREADWSNGARETARVILDHIAARARASKYREVRTRFPGCDAALLKEAHDRFGVVSPFGGPTSSGMVTLHCPPDRLYALASFLRLHGADTVSIASLDYVFDRDNPLFEKLEVFLRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPH8</accession>
    <name>Q3SPH8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2560</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase U62 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2560"/>
    <dbReference type="EnsemblBacteria" id="ABA05813">
        <property type="protein sequence ID" value="ABA05813"/>
        <property type="gene ID" value="Nwi_2560"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2560"/>
    <dbReference type="eggNOG" id="COG0312">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026425_0_0_5"/>
    <dbReference type="OMA" id="LYRKSTF"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045570">
        <property type="entry name" value="Metalloprtase-TldD/E_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002510">
        <property type="entry name" value="Metalloprtase-TldD/E_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035068">
        <property type="entry name" value="TldD/PmbA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01523">
        <property type="entry name" value="PmbA_TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19290">
        <property type="entry name" value="PmbA_TldD_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="SSF111283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PmbA_TldD" evidence="2">
        <location>
            <begin position="58"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="PmbA_TldD_M" evidence="4">
        <location>
            <begin position="157"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="PmbA_TldD_C" evidence="3">
        <location>
            <begin position="263"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF19290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="479" mass="50244" checksum="F5D3629E60F5827D" modified="2005-10-11" version="1">MTDQPFGGSCRRPWILFVNSSPNSSPESVASDLFDQSALSELAGRLVEAAKRAGADAADAIAVRGISQGVEVRDGRVEESERSEGDDVGLRVLVGRRQATVSTNDIGGDNVAKLAERAVAMARVAPDDQYVGLADPSLLAQNFPDLDLLDRVMPSTAELERRAVEAEAAGLAVKGVTKSGGASASTGISGMVLVTSTGFHGRYLRSSQGVSMMAIVGDGTGMERDYDFSSALHGSDLMPAASIGRSAGERAVARAGPRKVATCKVPVVFDPRVAGSLVSHVVSAANGAAVARKTSFLKDRLGQQLFAKGIRIIDDPLRVRGLRSQSFDAEGVATKKLAVIDDGVLTSWLLDSATARELGLTTTGHAHRGVSSSPSPGPYNLHLEAGEMTPRQLIAEVEQGFYVTDLIGSGVNGVTGDYSRGASGFWIENGEITYPVSEITIAGHLIEMFKSLTPANDLTFRYGTNAPTVRIEGLTVGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPI2</accession>
    <name>Q3SPI2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">3-deoxy-D-manno-octulosonic acid transferase</fullName>
            <shortName evidence="10">Kdo transferase</shortName>
            <ecNumber evidence="4 10">2.4.99.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 10">Lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_2556</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 10">Involved in lipopolysaccharide (LPS) biosynthesis. Catalyzes the transfer of 3-deoxy-D-manno-octulosonate (Kdo) residue(s) from CMP-Kdo to lipid IV(A), the tetraacyldisaccharide-1,4'-bisphosphate precursor of lipid A.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>CMP-3-deoxy-beta-D-manno-octulosonate + lipid IVA (E. coli) = alpha-Kdo-(2-&gt;6)-lipid IVA + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28066"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58603"/>
            <dbReference type="ChEBI" id="CHEBI:60364"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:85987"/>
            <dbReference type="EC" id="2.4.99.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Bacterial outer membrane biogenesis; LPS core biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the glycosyltransferase group 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.12" evidence="4 10"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315760.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2556"/>
    <dbReference type="CAZy" id="GT30">
        <property type="family name" value="Glycosyltransferase Family 30"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05809">
        <property type="protein sequence ID" value="ABA05809"/>
        <property type="gene ID" value="Nwi_2556"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2556"/>
    <dbReference type="eggNOG" id="COG1519">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036146_1_1_5"/>
    <dbReference type="OMA" id="FIKYEFW"/>
    <dbReference type="OrthoDB" id="1163086at2"/>
    <dbReference type="UniPathway" id="UPA00958"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043842">
        <property type="term" value="F:Kdo transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009244">
        <property type="term" value="P:lipopolysaccharide core region biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11720">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007507">
        <property type="entry name" value="Glycos_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038107">
        <property type="entry name" value="Glycos_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039901">
        <property type="entry name" value="Kdotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42755">
        <property type="entry name" value="PTHR42755"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04413">
        <property type="entry name" value="Glycos_transf_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0448">Lipopolysaccharide biosynthesis</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycos_transf_N" evidence="11">
        <location>
            <begin position="36"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="9">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="9">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR639901-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR639901-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA05809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="430" mass="46845" checksum="B02C994FAF3A1E0C" modified="2005-10-11" version="1">MPATLRLYRRLAVAAGPLAPLLLKRRLKQGKEDPARINERRGVARQPRPAGPLVWIHGASVGEVLAAAALIERLRALDIGVLVTSGTVTSAAVAAKRFPPDIIHQYIPYDSPRFVERFLDHWQPGLALFIESDLWPNLILAGASRRVPIVLINGRMSRRSFPRWRRASRTIGALLSRFDICLVQSEIDAERFTALGGRNVVITGNLKLDVQPPPADETRLERLLFVTRGRPVVVAASTHPGEEEILLNAHQTLVRDFPSLLTVIVPRHPDRGEAIARMVIASGAQAARRSLEQLPTARTNIYIADTIGEMGLFYRLAPIVFMGGSLVPHGGQNPIEAVKLKASIVHGPHVFNFPDVYGALDSAGGACRVDDGDALTKQLGYLLNDSAARDATATAASQVVEKLAGALDRTLAALEPYLLQLRLERRAADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPT0</accession>
    <name>Q3SPT0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Extensin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2458</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315666.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2458"/>
    <dbReference type="EnsemblBacteria" id="ABA05711">
        <property type="protein sequence ID" value="ABA05711"/>
        <property type="gene ID" value="Nwi_2458"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2458"/>
    <dbReference type="eggNOG" id="COG3921">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_743606_0_0_5"/>
    <dbReference type="OMA" id="QISAYSC"/>
    <dbReference type="OrthoDB" id="1439437at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009683">
        <property type="entry name" value="Extensin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06904">
        <property type="entry name" value="Extensin-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Extensin-like_C" evidence="2">
        <location>
            <begin position="180"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="335"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="372" mass="39660" checksum="949A4ED69EC29AB8" modified="2005-10-11" version="1">MTRRVALYLLGSLVLVSLAGCGRGLFQTAEREPWRTEAEAACLKSGAVRESSDLVRIRRINGPGICGAEFPLKVSALGEGSAYGFADENLRPPASVGREPRWPIAQPAYRPPSYPETSARQPGPDERSGDPVSLAAPGTYSSADEQPAEAGDDRYAQPSAYPATPSQDPSLRLGPARGVVTSAVGPVTVKPSATLACPIVSALDRWLADAVQPASMRWFGARVAEIKQISAYSCRGMNGNPRARISEHAFGNALDIAGFTLADGRYVSVQRGWKGLPEEQGFLRDVAASACQRFTTVLAPGSNIYHYNHIHVDLMRRASRRTVCKPAPVSGEEMAARAGRGSYAGRAPAVTGSLGASRNRSHSYASEADYDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPZ0</accession>
    <name>Q3SPZ0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Acylneuraminate cytidylyltransferase</fullName>
            <ecNumber evidence="1">2.7.7.43</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2397</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.43" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315612.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2397"/>
    <dbReference type="EnsemblBacteria" id="ABA05651">
        <property type="protein sequence ID" value="ABA05651"/>
        <property type="gene ID" value="Nwi_2397"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2397"/>
    <dbReference type="eggNOG" id="COG1083">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042930_1_1_5"/>
    <dbReference type="OMA" id="IFQFRPT"/>
    <dbReference type="OrthoDB" id="1345588at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008781">
        <property type="term" value="F:N-acylneuraminate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003329">
        <property type="entry name" value="Cytidylyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02348">
        <property type="entry name" value="CTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="249" mass="27521" checksum="49421F2F0B5AF265" modified="2005-10-11" version="1">MPDSADILFLIVGRGGSKGLPGKNLKQIGNLSLVGYRARSALKSRYCSRLIVSSDSPDIQAEAKRHGAEVLFSRPAELASDTASSADVVLHAMDWIEKHEGRRYGAVMLLEPSSPFARPEHYDQAVELYRARQAELVVGMRETEVSSVFVGQIGSDGSIAPIVDKMLSLSHLRRQDQPPEVTMNGALYLIDWNAMRKHRKIYANPSSSYGILMDRFHSIEIETMADLAYASYVIDHGMLDASPWQSTSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ87</accession>
    <name>Q3SQ87_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">AMP-dependent synthetase and ligase</fullName>
            <ecNumber evidence="4">6.2.1.26</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2300</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.26" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2300"/>
    <dbReference type="EnsemblBacteria" id="ABA05554">
        <property type="protein sequence ID" value="ABA05554"/>
        <property type="gene ID" value="Nwi_2300"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2300"/>
    <dbReference type="eggNOG" id="COG0318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_670490_0_0_5"/>
    <dbReference type="OMA" id="EIVVKMN"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008756">
        <property type="term" value="F:o-succinylbenzoate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="2">
        <location>
            <begin position="104"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="3">
        <location>
            <begin position="326"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="423" mass="45526" checksum="E515D316DDB6D999" modified="2005-10-11" version="1">MMIRIDDTFYDQARMQAMSERAALAVGIADQPGLSYAVCLSDTAEFLALFFALRSAGASVLPIRPSTPRAAARRLAVSVGCHRLVCDGFAVEELEGGAAGPGLLLQTTSGTTGEPKRVARTWAEIDREIESYVSAFREPESMTPLVACPVTHSYGLICGVLVALRRGQSPHVLNTANPRYVIARLRETRQPLLYSSPAMLHTISRLLPGGETIFAAMTSGTLLPDPWFASIRARTTHLFQQYGCSEAGCVAINPDLATPDEMGIPLPHLGVETGGEGAPREIVVRAGDREIHTRDLGYLTGTGTLVFVARLDDTINVSGINVYPREVEDVVMAMPGVTDAVAFRRVDPSAGERVALAFCAEHAIAPASLREWCMQNLAVHQVPMEAVRLACVPRQANGKINRREIAALYAEGRLEHAAAEAAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQD0</accession>
    <name>Q3SQD0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2257</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2257"/>
    <dbReference type="EnsemblBacteria" id="ABA05511">
        <property type="protein sequence ID" value="ABA05511"/>
        <property type="gene ID" value="Nwi_2257"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2257"/>
    <dbReference type="eggNOG" id="COG4544">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065750_2_0_5"/>
    <dbReference type="OMA" id="ACRFREP"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017026">
        <property type="entry name" value="ImuA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF034285">
        <property type="entry name" value="UCP034285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="270" mass="29005" checksum="927B6C6FA860BCFE" modified="2005-10-11" version="1">MPGTGSSAGKLARLRDRIARIETCHGGAMLGRAALDHAEMDAVLQGGLSRGAVHEVFAEGRHSAAATGFIAGLARRICARRPLLWVRQDFAARESGALSMCGWRELGLDPRLLVMVHVADAESALRTASDALACDALGAVVMEIWGETRLFDLVASRRLTLAARHSGVTGLLLRVAATPLPSTAETRWIVRPAASWPCAAWQAWGAPVFEARLVRNRHGQTGQWIMEWKCDECLFRNASCSGPQAHPQPVVAAPAGRPYPAKVVPLRRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQE0</accession>
    <name>Q3SQE0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2247</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315469.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2247"/>
    <dbReference type="EnsemblBacteria" id="ABA05501">
        <property type="protein sequence ID" value="ABA05501"/>
        <property type="gene ID" value="Nwi_2247"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2247"/>
    <dbReference type="eggNOG" id="COG5662">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076027_0_0_5"/>
    <dbReference type="OMA" id="GRFMAVP"/>
    <dbReference type="OrthoDB" id="1789373at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="230" mass="24148" checksum="9097DAAAD2DC5318" modified="2005-10-11" version="1">MTVAKRVADGGPGDVEGLLPWHAAGTLSARDTRRVEEALASDPGLAKQYAVIRDEYAETISLNESLGAPSAGAMHKLFAAIEAEPAARVPVARVGLLARLSRFFGDLSPRALAAAGAVGALVVVLQAGVIAAMLAKDDGGQSFQTASHRAPDAPLTRGLSVESGPQALVRFAPDARMSDIAEFLDSYQVTVVSTSRNGLFRLRFDQHKDLPGLLSKLQGERIVNLAVPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQE6</accession>
    <name>Q3SQE6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pyrrolidone-carboxylate peptidase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">5-oxoprolyl-peptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Pyroglutamyl-peptidase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_2241</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315463.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2241"/>
    <dbReference type="EnsemblBacteria" id="ABA05495">
        <property type="protein sequence ID" value="ABA05495"/>
        <property type="gene ID" value="Nwi_2241"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2241"/>
    <dbReference type="eggNOG" id="COG2039">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043960_4_1_5"/>
    <dbReference type="OMA" id="GRYLCNA"/>
    <dbReference type="OrthoDB" id="1927224at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016920">
        <property type="term" value="F:pyroglutamyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00501">
        <property type="entry name" value="Peptidase_C15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000816">
        <property type="entry name" value="Peptidase_C15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016125">
        <property type="entry name" value="Peptidase_C15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036440">
        <property type="entry name" value="Peptidase_C15-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23402">
        <property type="entry name" value="PTHR23402"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01470">
        <property type="entry name" value="Peptidase_C15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015592">
        <property type="entry name" value="Prld-crbxl_pptds"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00706">
        <property type="entry name" value="PYROGLUPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53182">
        <property type="entry name" value="SSF53182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="213" mass="23243" checksum="A123C8DFF2353F1A" modified="2005-10-11" version="1">MTTSLRVLITGFGPFPGAPFNPTVPLVKQLARLRRPALDDVTITTHIFDVAYATVDSELPRLLATHRPHALLMFGLAARTRWLRIETRARNAMTTIWPDQHGARIRKGAIAGTANARAFGPHVFKLLHAARRTGIDARASRDAGSYLCNYLCWRAVEAADHQAGPRLAAFIHVPALKRDGSAAVPGGMTLAHLVDAGEAMLMEMVKAARQTRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQG0</accession>
    <name>Q3SQG0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, MucR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2227</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ros/MucR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315449.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2227"/>
    <dbReference type="EnsemblBacteria" id="ABA05481">
        <property type="protein sequence ID" value="ABA05481"/>
        <property type="gene ID" value="Nwi_2227"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2227"/>
    <dbReference type="eggNOG" id="COG4957">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106247_2_1_5"/>
    <dbReference type="OMA" id="REKSIIC"/>
    <dbReference type="OrthoDB" id="1797085at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1550">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041920">
        <property type="entry name" value="ROS/MUCR_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008807">
        <property type="entry name" value="ROS_MUCR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05443">
        <property type="entry name" value="ROS_MUCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="137" mass="15366" checksum="0CDFBC82393B23E9" modified="2005-10-11" version="1">MSDSASKSFLDLTAEIVSAYLSNNTTPASEIPALINQVHAALTRVSSGRGELPAEPAKPAVPVRKSMTADYLICLEDGKRFKSLKRHLRTQYNMSPEQYRDKWKLPADYPMVAPNYAVTRSQLAKNMGLGQQRRRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQH7</accession>
    <name>Q3SQH7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Amino acid/amide ABC transporter membrane protein 2, HAAT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_2210</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2210"/>
    <dbReference type="EnsemblBacteria" id="ABA05464">
        <property type="protein sequence ID" value="ABA05464"/>
        <property type="gene ID" value="Nwi_2210"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2210"/>
    <dbReference type="eggNOG" id="COG4177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031365_1_1_5"/>
    <dbReference type="OMA" id="VFWYKLQ"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06581">
        <property type="entry name" value="TM_PBP1_LivM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021807">
        <property type="entry name" value="LivHM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043428">
        <property type="entry name" value="LivM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30482">
        <property type="entry name" value="PTHR30482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11862">
        <property type="entry name" value="DUF3382"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="338"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="373"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="410"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3382" evidence="7">
        <location>
            <begin position="15"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA05464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="456" mass="49449" checksum="EBC3BA8B84D6A6DF" modified="2005-10-11" version="1">MSQQTATRDRGVAAIIKNAVVSALLALVLFSLMIGIRTETGPSGQLTYWTRFGELAAVVGAVFIGSIIVETIKRWTGPVGSVELPDTVRRAFAMLGRLLIPALLIFALLVPVIFYNQRYLLDLAILVLTYVMLGWGLNVVVGLAGLLDLGYVAFYAVGAYSYALLAQSGLPFPYFIETYPYVAIDFLQLGSWAFWALLPVSGLLAAFWGVLLGFPVLRLRGDYLAIVTLAFGEIIRLVILNWQDLTGGPNGISGIPRPTVFGIPLTRGDDGFAALIGVPFSPTHRLVFLFYIILALALLTNWVTIRLRRLPIGRAWEAMREDEVACRALGINITTTKLSAFAIGAMFGGFAGAFFATRQGFISPESFTFQESALVLAIVVLGGMGSQLGVALAAIAMIGGFELFRGLDQFRMLVFGGVMVLLMIWRPRGLVSHRAPTVFLQKPTEISSSLVKEGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQI3</accession>
    <name>Q3SQI3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Molybdopterin dehydrogenase, FAD-binding protein</fullName>
            <ecNumber evidence="3">1.2.99.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2204</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.99.2" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315427.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2204"/>
    <dbReference type="EnsemblBacteria" id="ABA05458">
        <property type="protein sequence ID" value="ABA05458"/>
        <property type="gene ID" value="Nwi_2204"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2204"/>
    <dbReference type="eggNOG" id="COG1319">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058050_3_0_5"/>
    <dbReference type="OMA" id="YEFKYHR"/>
    <dbReference type="OrthoDB" id="1017413at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005107">
        <property type="entry name" value="CO_DH_flav_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036683">
        <property type="entry name" value="CO_DH_flav_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002346">
        <property type="entry name" value="Mopterin_DH_FAD-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00941">
        <property type="entry name" value="FAD_binding_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01092">
        <property type="entry name" value="CO_deh_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55447">
        <property type="entry name" value="SSF55447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="268" mass="28597" checksum="295DBA33209BA732" modified="2005-10-11" version="1">MYEFKYHRPDTLQQAVNLLAENEDAMLIAGGHTLVPVMKQRLASPAHLIDLSHIEGLDGIEMREGMLAIGATATHADVADSPIVREAIPALAELAAMIGDPAVRHKGTIGGSLANNDPTADYPAACLALGATIVTSKRRLESDDYFQGMFSTALDADEIIAQVMFPLPKQAAYQKFRNQASRYALVGVFVARLPSDVRVAVTGAGADGVFRLAAFEEALKKSFTSKSLEGLSIPSDGLNSDLHGSAEYRAHLIGLLARRAVDAANDRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQK5</accession>
    <name>Q3SQK5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Monooxygenase, FAD-binding protein</fullName>
            <ecNumber evidence="3">1.14.13.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2182</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.14.13.1" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315405.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2182"/>
    <dbReference type="EnsemblBacteria" id="ABA05436">
        <property type="protein sequence ID" value="ABA05436"/>
        <property type="gene ID" value="Nwi_2182"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2182"/>
    <dbReference type="eggNOG" id="COG0654">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009665_19_3_5"/>
    <dbReference type="OMA" id="SVWAIFD"/>
    <dbReference type="OrthoDB" id="504558at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018658">
        <property type="term" value="F:salicylate 1-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="FAD_binding_3" evidence="2">
        <location>
            <begin position="18"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="412" mass="44020" checksum="2A1ACA82F936022B" modified="2005-10-11" version="1">MRGEGQGRLIHPVRTLRTVVIAGAGIGGLTAALALAARGFRVLVIETAERLEEAGAGLQLSPNATRILIGLGLHERLSARALIPEAVSIMSVRHGGEICRLPLGEAAASRAGAPYWVMHRADLQAALLEQVHDHPGIELRLGANAEDAAAYATGANAEVCGGTTHPQETAWALIGADGVWSALRRRLFPNAAALFSGLIAWRGTVETAQLPHDMAAPRIQLWMGPDAHLVTYPMSGGRRINLVAVVPGGWTEPGWSAVGDAAEIQRAFAQASWPQPARLMIDAVDHWRKWALFTMPPMAAWHRDTTALLGDAAHAMLPFAAQGAGMAIEDAAVLARCLGEAANGNSESVASALARYAGLRRARVARMQDTAQLNGRIYHLRGVAASARDLAIRAMGPQRMLARQDWIYNWRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQL6</accession>
    <name>Q3SQL6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidyl-prolyl cis-trans isomerase</fullName>
            <ecNumber evidence="6">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_2171</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the FKBP-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2171"/>
    <dbReference type="EnsemblBacteria" id="ABA05425">
        <property type="protein sequence ID" value="ABA05425"/>
        <property type="gene ID" value="Nwi_2171"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2171"/>
    <dbReference type="eggNOG" id="COG0545">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013615_7_4_5"/>
    <dbReference type="OMA" id="RVIAGWD"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="5 6 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="7">
        <location>
            <begin position="61"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA05425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="154" mass="16237" checksum="C31C563CFD95199B" modified="2005-10-11" version="1">MRMLRHAGVLAFVLAIASGGVILIEASATAAAQTAEKTMTTASGLQITDTKVGDGPLPQPGQICIMHYTGWLYENGQKGKKFDSSVDRNEPFEFPIGKRRVIAGWDEGVATMKVGGKRTLIIPPELGYGARGAGGVIPPNATLIFDVELLGLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQP8</accession>
    <name>Q3SQP8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Antibiotic biosynthesis monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2139</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315362.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2139"/>
    <dbReference type="EnsemblBacteria" id="ABA05393">
        <property type="protein sequence ID" value="ABA05393"/>
        <property type="gene ID" value="Nwi_2139"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2139"/>
    <dbReference type="eggNOG" id="COG1359">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127577_4_1_5"/>
    <dbReference type="OMA" id="QWRRRED"/>
    <dbReference type="OrthoDB" id="1909149at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007138">
        <property type="entry name" value="ABM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03992">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51725">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABM" evidence="1">
        <location>
            <begin position="16"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="111" mass="12355" checksum="A2CC777325C1965F" modified="2005-10-11" version="1">MTDKKAATIDVRGGVVTLINVYEVEPDRQAELVRLLEEATETVMRHQPGFISVSIHSSFDGARVVNYAQWASKEDFERLMKNSEAQAQMKTFAATARSVAPALYRVSSVHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQU3</accession>
    <name>Q3SQU3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Conjugation TrbI-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2091</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TrbI/VirB10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315326.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2091"/>
    <dbReference type="EnsemblBacteria" id="ABA05348">
        <property type="protein sequence ID" value="ABA05348"/>
        <property type="gene ID" value="Nwi_2091"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2091"/>
    <dbReference type="eggNOG" id="COG2948">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042657_0_0_5"/>
    <dbReference type="OrthoDB" id="1674599at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16429">
        <property type="entry name" value="VirB10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005498">
        <property type="entry name" value="T4SS_VirB10/TraB/TrbI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042217">
        <property type="entry name" value="T4SS_VirB10/TrbI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03743">
        <property type="entry name" value="TrbI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="402" mass="43179" checksum="92B879B9E8274A1E" modified="2005-10-11" version="1">MTDASTPPPGDIRAELRLRPEQPRVTRLSRKVLITLGGVSAVAIMAALLWALDANRRASQSPNELYNTENRTPADGLAGLPKDYARIPKLGPALPGDLGRPILSAQDEGKPVVAPDIRTPRADPAEQRRLAEIEAARVAKVFTDSRGVQATVASAPAIGRVDPNTGLTLPTETPPLDAGAAQNMQDRKLAFVNAAVDRRTVTPDRVQEKASPYVVQAGTVIPAALITGIKSDLPGTITAQVTEQVYDTPSGKQLLIPQGARLIGQYDSQVAFGQSRVLLVWNRIIMPNGTSIVLERQPGADAEGYSGLEDDVDYHWGQLFRAAILSTLLGVGTEIGASNNENEIARAIRQSSQDTASDVGRQIIRRQLNIQSTLTIRPGFPVRVIVNRDLILQPYGATKEPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQW8</accession>
    <name>Q3SQW8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sulfate transporter/antisigma-factor antagonist STAS</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_2065</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Membrane</location>
            <topology evidence="1 5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SLC26A/SulP transporter (TC 2.A.53) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2065"/>
    <dbReference type="EnsemblBacteria" id="ABA05323">
        <property type="protein sequence ID" value="ABA05323"/>
        <property type="gene ID" value="Nwi_2065"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2065"/>
    <dbReference type="eggNOG" id="COG0659">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003182_13_1_5"/>
    <dbReference type="OMA" id="ALYWIPK"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008271">
        <property type="term" value="F:secondary active sulfate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="PTHR11814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SSF52091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="88"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="258"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="338"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="388"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="7">
        <location>
            <begin position="450"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="559"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="565"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA05323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="579" mass="61076" checksum="B3F3EA345C8E2526" modified="2005-10-11" version="1">MAPVEPTFVEQFTPKLITVLREGYGFKNFRADVLAGLTVAIVAIPLSMAIAIASGVSPDRGLYTAVVGGFVVSLLSGSRYQIGGPAGAFIVLVSVTVALHGVEGLILATLLSGFIQLAIGLLRFGTYIKYIPFPVTIGFTAGISVIIFASQLTELLGLTLAQKEPGELIPKLMAIGEALPTISTAAVGVSLLSIAVIVAIRRFRPRYPAMLIAVGIASVAVWALGLPVETIGTRFGGIPQTLPTPALPVISLEKITEVLPAALSFALLGSIESLLSAVVADSMSGHRHRSNCELMAQGFANIAAPLFGGFCVTGTIARTATNIRSGAHGPIAGMLHAVFLLLFMLIAAPLASFIPLAALGAVLAVVAWNMAERHAFAMLLRASRSDAVILMTTFLLVIFRDLIEGILVGFGIGALLFLHRMAQSVKVENARSPIDDDKSDTVDGNGRKSYDVDLDADPAVAVHRISGAFFFGAAANVGEELDRLDEHAKAYVIDFSEVSMIDSSGAATIEGVMHKAHRRKAPVYIVGARPAIRRTLLVHGVKPPYVRFRNDLTEAVGAAHNRTAHGRRRGAEEEEVAAA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-05-25" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR28</accession>
    <name>YQGF_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative pre-16S rRNA nuclease</fullName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">Nwi_2004</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Could be a nuclease involved in processing of the 5'-end of pre-16S rRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the YqgF nuclease family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315249.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SR28"/>
    <dbReference type="SMR" id="Q3SR28"/>
    <dbReference type="STRING" id="323098.Nwi_2004"/>
    <dbReference type="EnsemblBacteria" id="ABA05263">
        <property type="protein sequence ID" value="ABA05263"/>
        <property type="gene ID" value="Nwi_2004"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2004"/>
    <dbReference type="eggNOG" id="COG0816">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098240_1_1_5"/>
    <dbReference type="OMA" id="PMGWTAQ"/>
    <dbReference type="OrthoDB" id="2075502at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000967">
        <property type="term" value="P:rRNA 5'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16964">
        <property type="entry name" value="YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00651">
        <property type="entry name" value="Nuclease_YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005227">
        <property type="entry name" value="YqgF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006641">
        <property type="entry name" value="YqgF/RNaseH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037027">
        <property type="entry name" value="YqgF/RNaseH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33317">
        <property type="entry name" value="PTHR33317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03652">
        <property type="entry name" value="RuvX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00732">
        <property type="entry name" value="YqgFc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00250">
        <property type="entry name" value="RNAse_H_YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="chain" id="PRO_0000257555" description="Putative pre-16S rRNA nuclease">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00651"/>
        </source>
    </evidence>
    <sequence length="163" mass="17479" checksum="C46A22818432E252" modified="2005-10-11" version="1">MPASVLPLVEAAAHWPARGALVGLDLGTKTIGVAVSDPDRRLATGVETIRRKTFTADAARLLAIAGERRAEGFILGLPINMDGSEGPRAQSTRAFARNLARLTDFAIGLWDERLSTAAVERELIGLDMSRARRAKVIDEHAAIFILQGALDRLATQRRTSGPG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR31</accession>
    <name>GATA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamyl-tRNA(Gln) amidotransferase subunit A</fullName>
            <shortName evidence="1">Glu-ADT subunit A</shortName>
            <ecNumber evidence="1">6.3.5.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gatA</name>
        <name type="ordered locus">Nwi_2001</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the amidase family. GatA subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.7" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315246.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SR31"/>
    <dbReference type="SMR" id="Q3SR31"/>
    <dbReference type="STRING" id="323098.Nwi_2001"/>
    <dbReference type="EnsemblBacteria" id="ABA05260">
        <property type="protein sequence ID" value="ABA05260"/>
        <property type="gene ID" value="Nwi_2001"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2001"/>
    <dbReference type="eggNOG" id="COG0154">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009600_0_3_5"/>
    <dbReference type="OMA" id="EVSCPHF"/>
    <dbReference type="OrthoDB" id="1239251at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00120">
        <property type="entry name" value="GatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004412">
        <property type="entry name" value="GatA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="PTHR11895"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="SSF75304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00132">
        <property type="entry name" value="gatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000241124" description="Glutamyl-tRNA(Gln) amidotransferase subunit A">
        <location>
            <begin position="1"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="1">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00120"/>
        </source>
    </evidence>
    <sequence length="491" mass="52513" checksum="A46F16C0DB5FBE76" modified="2005-10-11" version="1">MTDLTSLTLAEARDGLARKSFTALELTDAHLAAMERARVLNAYVLETPERAREMARQADGRIAKSEGGPLNGLPLGVKDLFATEGTRTTACSRILGDFKPPYESTVTSQLWRDGAVMLGKLNNDEFAMGSSNETSCFGPVINPWRRQGSDVRLVPGGSSGGSASAVAAGICLGATATDTGGSIRQPAAFTGTVGLKPTYGRCSRWGTVAFASSLDQAGPIARTVRDAAILLRSMAGHDPKDTTSVDRAVPDYEAAVGRSVKGMKIGLPREYRLDGMPAEIEKLWSQGADWLKAAGAELVEVSLPHTKYALPAYYIVAPAEASSNLARYDGVRYGARADGSNIVEMYENTRAKGFGAEVRRRIMIGTYVLSAGYYDAYYLRAQKVRTLIKKDFEDCYEAGVDAILTPATPSAAFGIGEKTDSDPIEMYLNDIFTVTANMAGLPGIAVPAGRDAQGLPLGLQLIGRPFDEEAVISLGEVIEQAAGRFTPEVWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR71</accession>
    <name>Q3SR71_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH_17 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315211.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1960"/>
    <dbReference type="EnsemblBacteria" id="ABA05220">
        <property type="protein sequence ID" value="ABA05220"/>
        <property type="gene ID" value="Nwi_1960"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1960"/>
    <dbReference type="eggNOG" id="COG3311">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140176_9_5_5"/>
    <dbReference type="OMA" id="ARWNISH"/>
    <dbReference type="OrthoDB" id="2076478at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041657">
        <property type="entry name" value="HTH_17"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12728">
        <property type="entry name" value="HTH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_17" evidence="1">
        <location>
            <begin position="10"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="69" mass="8091" checksum="6DA35D7383C2C051" modified="2005-10-11" version="1">MATKHLNQIDLAARWNISHRTLERWRWTGEGPRFVKLGGRVVYRLEDVEEYEREQIRASTADIPTKPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR93</accession>
    <name>Q3SR93_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CRISPR-associated protein, Csd2 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1938</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315191.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1938"/>
    <dbReference type="EnsemblBacteria" id="ABA05198">
        <property type="protein sequence ID" value="ABA05198"/>
        <property type="gene ID" value="Nwi_1938"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1938"/>
    <dbReference type="eggNOG" id="COG3649">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071770_2_0_5"/>
    <dbReference type="OMA" id="YDFVLLF"/>
    <dbReference type="OrthoDB" id="598606at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006482">
        <property type="entry name" value="Cas7_Csh2/Csh2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013418">
        <property type="entry name" value="CRISPR-assoc_prot_Csd2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05107">
        <property type="entry name" value="Cas_Cas7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02589">
        <property type="entry name" value="cas_Csd2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01595">
        <property type="entry name" value="cas_CT1132"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="316" mass="35557" checksum="34B0CB2E60547FDF" modified="2005-10-11" version="1">MTMLTNRYDFVLLFDVTKGNPNGDPDAGNLPRLDPETNHGLVSDVSLKRKVRNYVDLVRSGTDGHHIYVEEAAILNDKHRQAYKALRPDDPKVDKEAKLNPRDDVEAKKLREFMCKNFFDVRTFGAVMSTGINAGQVRGPVQMTFANSVEPIVPQEISITRMAATNEAEKKQRAEGGEEGNDRVDNRTMGRKYIVPYGLYRAHGFVSAKLAERTGFSEADLELTFEALTSMFEHDRSAARGEMTTRKLVVFKHGNALGSAPAHALFERVRIGRNIDGQFRRIDRSDNYPPARAFSDYAVEIDRDNPPDGVEIIERI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRD0</accession>
    <name>Q3SRD0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Multicopper oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1901</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1901"/>
    <dbReference type="EnsemblBacteria" id="ABA05161">
        <property type="protein sequence ID" value="ABA05161"/>
        <property type="gene ID" value="Nwi_1901"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1901"/>
    <dbReference type="eggNOG" id="COG2132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009100_6_1_5"/>
    <dbReference type="OMA" id="INACQRN"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="PTHR11709"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="SSF49503"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plastocyanin-like" evidence="1">
        <location>
            <begin position="175"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="2">
        <location>
            <begin position="329"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="425" mass="45963" checksum="83C04A4B14C78506" modified="2005-10-11" version="1">MAGPAAPIYARSQDPQRLAIDATPAPLSLGPDHHETTVWSLRTGGSSRIPRFRQGESMDAVVTNGLPAPLVLNWRGVDGAAAIEPLTDRSPIPPGGTDRYAFPLRQAGTFMCDARLLGDGLAQPSPAQALIVEEREPIEADRDEVMLIEDWRFDAEGRPVAPGPTENQRKAGPPLYLVNGKPSLDLNLHVNERLRLRVINGCQRNAIAIAIQNLDVRIMAIDSQPAEPFLARNGRLVLAPGTRIDAIIDATLPAGTASAITLHDGGGTPRQIARLKVSDGPARSAPLPAPPALPSANLPARLDLRNALRTDVPLISLAGTSSWDRPALFSKRRAPAFRVKRGRTAVLALKNTTTTPMVFHLHGHHARLLDRLDDGWKPFWLDTVLVDAGQTQRIAFVADVAGAWLIETMAVEWSAPRQVGWFAVE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-07-11" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRF1</accession>
    <name>NUOH_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADH-quinone oxidoreductase subunit H</fullName>
            <ecNumber evidence="1">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">NADH dehydrogenase I subunit H</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">NDH-1 subunit H</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nuoH</name>
        <name type="ordered locus">Nwi_1880</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. This subunit may bind ubiquinone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I subunit 1 family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315136.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRF1"/>
    <dbReference type="SMR" id="Q3SRF1"/>
    <dbReference type="STRING" id="323098.Nwi_1880"/>
    <dbReference type="EnsemblBacteria" id="ABA05140">
        <property type="protein sequence ID" value="ABA05140"/>
        <property type="gene ID" value="Nwi_1880"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1880"/>
    <dbReference type="eggNOG" id="COG1005">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015134_0_1_5"/>
    <dbReference type="OMA" id="WSGWASN"/>
    <dbReference type="OrthoDB" id="1559067at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016655">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01350">
        <property type="entry name" value="NDH1_NuoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001694">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su1/FPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018086">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su1_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11432">
        <property type="entry name" value="PTHR11432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00146">
        <property type="entry name" value="NADHdh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00668">
        <property type="entry name" value="COMPLEX1_ND1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0874">Quinone</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0830">Ubiquinone</keyword>
    <feature type="chain" id="PRO_0000244925" description="NADH-quinone oxidoreductase subunit H">
        <location>
            <begin position="1"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="292"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01350"/>
        </source>
    </evidence>
    <sequence length="356" mass="39315" checksum="70D0259AB1571C75" modified="2005-10-11" version="1">MADFFAMPLWTDFLWPLIVMVAQSVLLLVVLLIAIAYILLADRKIWAAVQIRRGPNVVGPWGLFQSFADLLKFVLKEPIIPSGANKGVFLLAPLVSCVLALAAWAVIPVNLNWVIADINVGILYIFAISSLSIYGIIMGGWASNSKYPFLAALRSAAQMVSYEVSIGFVIITVLLCAGSLNLSAIVEAQHTSGLATLIGLPWLTFLNWYWLPLLPMFVVFYVSALAETNRPPFDLVEAESELVAGFMVEYGSTPYLLFMLGEYVAITTMCAMGAILFMGGWLPPVDLPPFNWVPGVIWFSLKLFFMFFLIAMAKAIVPRYRYDQLMRLGWKVFLPLSLAMVVIVAAVLQFAGIAPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRF4</accession>
    <name>Q3SRF4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NADH-quinone oxidoreductase subunit K</fullName>
            <ecNumber evidence="7">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">NADH dehydrogenase I subunit K</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">NDH-1 subunit K</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">nuoK</name>
        <name evidence="8" type="ordered locus">Nwi_1877</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell inner membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the complex I subunit 4L family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315133.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1877"/>
    <dbReference type="EnsemblBacteria" id="ABA05137">
        <property type="protein sequence ID" value="ABA05137"/>
        <property type="gene ID" value="Nwi_1877"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1877"/>
    <dbReference type="eggNOG" id="COG0713">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144724_2_0_5"/>
    <dbReference type="OMA" id="NFVAFSY"/>
    <dbReference type="OrthoDB" id="2081663at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050136">
        <property type="term" value="F:NADH dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01456">
        <property type="entry name" value="NDH1_NuoK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001133">
        <property type="entry name" value="NADH_UbQ_OxRdtase_chain4L/K"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039428">
        <property type="entry name" value="NUOK/Mnh_C1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11434">
        <property type="entry name" value="PTHR11434"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00420">
        <property type="entry name" value="Oxidored_q2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 7" id="KW-0874">Quinone</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0830">Ubiquinone</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="62"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA05137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="102" mass="10831" checksum="FC64644D4985D8F0" modified="2005-10-11" version="1">MTIGLGHYLAVGAILFTLGTLGIFLNRKNIIIILMSVELILLAVNINLVAFSTFLGDIVGQVFTLLVLTVAAAEAAIGLAILVVYFRNRGSIAVEDVNLMKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRH0</accession>
    <name>Q3SRH0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1861</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05121">
        <property type="protein sequence ID" value="ABA05121"/>
        <property type="gene ID" value="Nwi_1861"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1861"/>
    <dbReference type="HOGENOM" id="CLU_2383212_0_0_5"/>
    <dbReference type="OMA" id="ATAWWSY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="94" mass="10609" checksum="0B0D179EE1118671" modified="2005-10-11" version="1">MVLIPSDKRTKQLAEWTGGRYRAVMAQKLKPGVRARDGGEYTCPGCGAVYSVTVYTSPFKDTGQADCKVCNQPIKSWNHATAWWSYKLIKRPRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRH6</accession>
    <name>Q3SRH6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Isoprenyl transferase</fullName>
            <ecNumber evidence="2">2.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1855</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the condensation of isopentenyl diphosphate (IPP) with allylic pyrophosphates generating different type of terpenoids.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315111.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1855"/>
    <dbReference type="EnsemblBacteria" id="ABA05115">
        <property type="protein sequence ID" value="ABA05115"/>
        <property type="gene ID" value="Nwi_1855"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1855"/>
    <dbReference type="eggNOG" id="COG0020">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038505_1_1_5"/>
    <dbReference type="OMA" id="PRTEGHK"/>
    <dbReference type="OrthoDB" id="1630604at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00475">
        <property type="entry name" value="Cis_IPPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01139">
        <property type="entry name" value="ISPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018520">
        <property type="entry name" value="UPP_synth-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291">
        <property type="entry name" value="PTHR10291"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="SSF64005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00055">
        <property type="entry name" value="uppS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01066">
        <property type="entry name" value="UPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2 3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="27"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="71"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="200"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="26"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="74"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="252" mass="27977" checksum="E6B56B605858FB3D" modified="2005-10-11" version="1">MSNAVAPAIEGSDQADAPLHVAIIMDGNGRWAAARGLPRAEGHRRGVEALRRVVRAAGELNILYLTIFSFSSENWSRPPSEISDLFGLLRRFVRNDLATLHRDGVRVRVIGERDGLEPDISALLTEAEELTKNNNRLNLVVAFNYGSRQEIAKAARRLAREVAEGKRDPATIDADTLGRYLDAPDIPDPDLIIRTSGEQRLSNFLMWQAAYSEFVFVPIHWPDFDRTALEGAIAEYARRERRFGGLAAKAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRP1</accession>
    <name>Q3SRP1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase, family 2</fullName>
            <ecNumber evidence="3">2.4.1.12</ecNumber>
            <ecNumber evidence="3">3.2.1.58</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1789</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.1.12" evidence="3"/>
    <dbReference type="EC" id="3.2.1.58" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315046.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1789"/>
    <dbReference type="CAZy" id="GH17">
        <property type="family name" value="Glycoside Hydrolase Family 17"/>
    </dbReference>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05050">
        <property type="protein sequence ID" value="ABA05050"/>
        <property type="gene ID" value="Nwi_1789"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1789"/>
    <dbReference type="eggNOG" id="COG1215">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016454_0_0_5"/>
    <dbReference type="OMA" id="FRWAYGA"/>
    <dbReference type="OrthoDB" id="926333at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016760">
        <property type="term" value="F:cellulose synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004338">
        <property type="term" value="F:glucan exo-1,3-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229279" evidence="2">
        <location>
            <begin position="17"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="335"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="361"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="679"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="714"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="791"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="820"/>
            <end position="843"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="889" mass="98027" checksum="219871F4B911708C" modified="2005-10-11" version="1">MRAVVAVFLFVSAAHAALWGVLQDKQSAPDFKGVLTSLSYAPFEGAAHPDVDNIPTVEKIRTDMKALAPLTRAIRLYSSTGGVELVPPIANEFGLKVMVGAWIDKFVERNEREMLAAIDLAKHNGNVNGIVVGNETIYRGDQKVSDLIKLIQRVKGSVNVPVTTGEIWNIWLEHPELASSVDFIAAHILPYWEGFSSKQAVDQALIIYQKLRDAFPGKRIVIAEFGWPSAGYNLRNADPGPFQQAATLRNFVTKAQSIGMEYNIVEAIDQPWKFFEGGVGPYWGILNADREPKFSWSGPVVNEAYWKLAGIALLIGILLSLPILRLVRPTPMQTLLLSVAANGVGAWVATVFAYWTTHYFVFGSALALALGLTLLVPLILIAMARIEEIATVAFGERPRRLITREAAAAAQAASDAPLPKVSIHVPAYFEPPEMLKQTLDAVARLDYPNFECVVIINNTPDPAFWQPIQDHCRALGERFKFINAEKVEGFKAGALRIAMERTAADAEIIGVIDADYVVQPDWLKDLVPAFNDPGVGLVQAPQDHRDGDRSLMHYIMNGEYAGFFDIGMVQRNETNAIIVHGTMCLIRRAAMDMAGGWAGDTICEDTDLGLAIAQHGWVTHYTNTRYGYGLLPDTYEAFKKQRHRWAYGGFQIVKKHWRRFLPGASRLSPDQKREFTLGWLNWLGAESLGVVVAILNLIWVPIVAFADIAIPDKILTLPIIAAFVVSLLHFLTLYRLRVPIKTAQMLGAMVAAMSVQWTVSRAVAQGLITEHLAFARTSKGGLSRMSIEFQAFWEAVIGVLLLVGAAVLIASNQYKEVTEIYIFAGVLVLQSLPFLSAVAIAILEMSRINQFSFWRNARIRSAELIGLRPAPMATGTSSPVGSEIQRETT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-10-14" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRQ7</accession>
    <name>PIMT_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein-L-isoaspartate O-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.77</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">L-isoaspartyl protein carboxyl methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Protein L-isoaspartyl methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Protein-beta-aspartate methyltransferase</fullName>
            <shortName evidence="1">PIMT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pcm</name>
        <name type="ordered locus">Nwi_1773</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-L-isoaspartate + S-adenosyl-L-methionine = [protein]-L-isoaspartate alpha-methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:12705"/>
            <dbReference type="Rhea" id="RHEA-COMP:12143"/>
            <dbReference type="Rhea" id="RHEA-COMP:12144"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90596"/>
            <dbReference type="ChEBI" id="CHEBI:90598"/>
            <dbReference type="EC" id="2.1.1.77"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.77" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315030.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRQ7"/>
    <dbReference type="SMR" id="Q3SRQ7"/>
    <dbReference type="STRING" id="323098.Nwi_1773"/>
    <dbReference type="EnsemblBacteria" id="ABA05034">
        <property type="protein sequence ID" value="ABA05034"/>
        <property type="gene ID" value="Nwi_1773"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1773"/>
    <dbReference type="eggNOG" id="COG2518">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055432_2_0_5"/>
    <dbReference type="OMA" id="TISAIHM"/>
    <dbReference type="OrthoDB" id="1948290at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004719">
        <property type="term" value="F:protein-L-isoaspartate (D-aspartate) O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030091">
        <property type="term" value="P:protein repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00090">
        <property type="entry name" value="PIMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000682">
        <property type="entry name" value="PCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579">
        <property type="entry name" value="PTHR11579"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00080">
        <property type="entry name" value="pimt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000351884" description="Protein-L-isoaspartate O-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00090"/>
        </source>
    </evidence>
    <sequence length="217" mass="24159" checksum="DB7076350B94A3FD" modified="2005-10-11" version="1">MPPADHPPPEKMMFQLNLRRRGISDQRVLRAMDNVPRDAFVEQGDREDAWCDTALGIACGQTISQPFVVAYMTERLELGDDQRVLEIGTGSGYQTAILSRLCREVVTVERYRVLADRARARLKHLGYDNVEVLLGDGFDIPGEAGRFDRIMVTAAMEQIPEALTARLEPDGLLIAPVGPQSGRQTLILLRRAPAGMIRKELIDVRFVPALPGIAREL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRR0</accession>
    <name>Q3SRR0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 12">Sec translocon accessory complex subunit YajC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">yajC</name>
        <name evidence="14" type="ordered locus">Nwi_1770</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 12">The SecYEG-SecDF-YajC-YidC holo-translocon (HTL) protein secretase/insertase is a supercomplex required for protein secretion, insertion of proteins into membranes, and assembly of membrane protein complexes. While the SecYEG complex is essential for assembly of a number of proteins and complexes, the SecDF-YajC-YidC subcomplex facilitates these functions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 12">Part of the SecDF-YidC-YajC translocase complex. The SecDF-YidC-YajC translocase forms a supercomplex with SecYEG, called the holo-translocon (HTL).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell inner membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the YajC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315027.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1770"/>
    <dbReference type="EnsemblBacteria" id="ABA05031">
        <property type="protein sequence ID" value="ABA05031"/>
        <property type="gene ID" value="Nwi_1770"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1770"/>
    <dbReference type="eggNOG" id="COG1862">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116157_2_0_5"/>
    <dbReference type="OMA" id="GMEMIIM"/>
    <dbReference type="OrthoDB" id="2082059at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003849">
        <property type="entry name" value="Preprotein_translocase_YajC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33909">
        <property type="entry name" value="PTHR33909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02699">
        <property type="entry name" value="YajC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01853">
        <property type="entry name" value="YAJCTRNLCASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01323">
        <property type="entry name" value="YajC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00739">
        <property type="entry name" value="yajC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0811">Translocation</keyword>
    <keyword evidence="9 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="103"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00014962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU366028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABA05031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="133" mass="14234" checksum="13958DF8128B332B" modified="2005-10-11" version="1">MFITPAFAQAAGSGDANSLLMSLLPFALIFIIMYFLIIRPQQKKAKEHAELVKNIRRGDTVITSSGLVGKVTKVVDDEQIEFEIADGVRVRQMRQMISIVRAKGEPAKGEAAKGAAKADGAKGNGSRDDTAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRS6</accession>
    <name>Q3SRS6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_1754</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1754"/>
    <dbReference type="EnsemblBacteria" id="ABA05015">
        <property type="protein sequence ID" value="ABA05015"/>
        <property type="gene ID" value="Nwi_1754"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1754"/>
    <dbReference type="eggNOG" id="COG4251">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5278">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_71_5"/>
    <dbReference type="OMA" id="SSTRKMD"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007891">
        <property type="entry name" value="CHASE3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05227">
        <property type="entry name" value="CHASE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="5">
        <location>
            <begin position="281"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="538" mass="60088" checksum="D294DFC5157674B3" modified="2005-10-11" version="1">MRAEGFSKRPVNARGRFRLSRSEYDEIVAPRARTSRPALSQLLLLTAGLVVLTLISVISIYLVNKARDDTRWVLRSMEVENEISLAQLQIQRAESSQRSFLLTQRPEFRAAFEDASGRIPLSFERLRVLTKENPYQQQRLQQMVPLTSQRIEQLRQSIDLALTNRLDQAVESARREAGQDATRQIRDLGNEMRTEANRLLAARTASADASQSRSALITSVGSGLVVLFAGLSIYLVRRSNRERDLANARLRDINLNLESTIEKRTSDLREANDEIQRFAYIVSHDLRSPLVNIMGFTSELEELRGDIFRRIAELSRPDSLPPPGVNDPASAAEPVLDGPDEQLSQDFTEALGFIKSSIAKMDRLIGAILSLTREGRREFHPVSIAMRDLIEGIVSTVAHQAAEADAQIRVEPLPDIVTDRIAIEQIFSNLIDNALKYLKPDVPGDIAISGRRKLGFAIFEITDNGRGIDPKDHQRIFDLFRRAGPQDRPGQGIGLAHVRALVRRLGGTISVSSKLGAGTTFTVTLPSTWMVTNRDEQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRU0</accession>
    <name>Q3SRU0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Adenylate/guanylate cyclase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1740</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314997.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1740"/>
    <dbReference type="EnsemblBacteria" id="ABA05001">
        <property type="protein sequence ID" value="ABA05001"/>
        <property type="gene ID" value="Nwi_1740"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1740"/>
    <dbReference type="eggNOG" id="COG2114">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4252">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_85_1_5"/>
    <dbReference type="OMA" id="RFNYSAM"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009190">
        <property type="term" value="P:cyclic nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007890">
        <property type="entry name" value="CHASE2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05226">
        <property type="entry name" value="CHASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01080">
        <property type="entry name" value="CHASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="386"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="415"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="2">
        <location>
            <begin position="504"/>
            <end position="642"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="753" mass="81084" checksum="8C932D4CDB416346" modified="2005-10-11" version="1">MKAATRLRNTLRVRAMLAWARLRRLTRNKNLRAVGAAVVTLVLLASLRAYDPRIVTELKERTFDTYQRLKPRAYTDLPVRIVDIDEASISEYGQWPWPRTRLAALTRRLADLGAGVVAYDVIFSEPDRTTPSRLANDLKDSGAPDLDQTLKLLANLPDHDQAFADAIENAGVVFGFAATGAVNDKRPPVRAGVAFVGVNPANVLAPFGGTVSSLPILNQKAAGIGGINLSSHDRGGIVRRVPMLFSDGVSIYPSLAIEALRVAQNQKSIIVRGTGSSGDSDTGSAALIDMRVGAFKLPLTSNGESWVYFSRDRPQRYVSVKDILDPAKEATVKPLVDGAIVLVGASAAGLMDSRTTPLGGSVPGVSVQAQVIEQILAQDFIERPDWAKGLEIVLTLLFGALVAGLVLMFGARFSFYAGVVGFVAGLAGSWIAFSHFRLLIDPIFPSLAALTVYIAVERVLHVASDREKKFVRQAFGQYLAPELLTQLENSPQEMRLGGASRDISVMFMDVRGFTPISEALTATELVDFINTLLAPLSDAIQDELGTIDKYIGDSIMAFWNAPADVPDHATRACQAALKMREALTALNDADVFGFSARGFTDPRVRIGVGINAGLACVGNMGSQRRFNYSAMGDVVNVAARIESASKTLDVDLLVSEDVARRVRGIALLEAGEILLKGKSRPTKIYAIVGDDSVAASPEFAEWKARHGALLAAIKAGEGFEATAALARCRELAGEDMQGFYHRLAEQIVPIHTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRV1</accession>
    <name>Q3SRV1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4062 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1729</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314986.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1729"/>
    <dbReference type="EnsemblBacteria" id="ABA04990">
        <property type="protein sequence ID" value="ABA04990"/>
        <property type="gene ID" value="Nwi_1729"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1729"/>
    <dbReference type="eggNOG" id="ENOG5031ZXI">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062193_1_1_5"/>
    <dbReference type="OMA" id="DIFAFEY"/>
    <dbReference type="OrthoDB" id="1024184at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025139">
        <property type="entry name" value="DUF4062"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13271">
        <property type="entry name" value="DUF4062"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4062" evidence="2">
        <location>
            <begin position="6"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="169"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="333" mass="37365" checksum="3359C08184C75A22" modified="2005-10-11" version="1">MDRVFQIFIASVDADLKERQAVGNTLAKAGYMPAGMESFPVTDQQQLDYIKRIIDRSDYYIIIVGRRYGSLSDHELIFAEDVFAYARSKDIPILAFLPEKPGHATGGAIEPEPPLKERLDTFKARLSTSRIVEFWSNENDLCMKAMMAVATAVNLKPGVGWIRGDQAVNPKLFQELEKLRTENADLRQKLADLDRDKSSSHPAEVEPDNSVTVEFLVYRSEEGAPDSISRPVLLGDLFTGLYGHLLKSPSEAYVRELVGYWYRQKLHISDYCELSEKSAVIIREKLEALGLIKPRSIMAGFSNHVAWSLTEKGRRFSESGHLPRIGHSGSTAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRX4</accession>
    <name>Q3SRX4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glutathione S-transferase</fullName>
            <ecNumber evidence="2">2.5.1.18</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1706</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.5.1.18" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314965.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1706"/>
    <dbReference type="EnsemblBacteria" id="ABA04967">
        <property type="protein sequence ID" value="ABA04967"/>
        <property type="gene ID" value="Nwi_1706"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1706"/>
    <dbReference type="eggNOG" id="COG0625">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070658_0_0_5"/>
    <dbReference type="OMA" id="CPMNIEA"/>
    <dbReference type="OrthoDB" id="1512459at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="SSF47616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="1">
        <location>
            <begin position="3"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="218" mass="24627" checksum="D2BFDCBCA55A5CDD" modified="2005-10-11" version="1">MTLTLVIGNKNYSSWSMRPWLALRAGGIAFKEKLIPLYTGPADKQRILDINPAGKVPALIDGRVIVWDSLAIIEYAAERFPEAGLWPADPASRAHARSISAEMHSGFSALRRECGMNLHRPISPKTLSDSARADIARIQELWTYCRRRSERDGPFLFGSFTAADAMYAPVIHRFRTYAIEVTTQVQEYMNAMMEQPAFREWTGQGLAETMVIERFETD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS73</accession>
    <name>Q3SS73_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1607</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1607"/>
    <dbReference type="EnsemblBacteria" id="ABA04868">
        <property type="protein sequence ID" value="ABA04868"/>
        <property type="gene ID" value="Nwi_1607"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1607"/>
    <dbReference type="HOGENOM" id="CLU_2106387_0_0_5"/>
    <dbReference type="OMA" id="AFGRISC"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="116" mass="12092" checksum="181D1B2E12D6E2BD" modified="2005-10-11" version="1">MSSWTLGARKPPQGDGLEIDIAIDGGDPLTLNTLEAETLETGLGKALAGVDNASGRISCVNAATQIIVAVDKVTVASLRYEKAERLRGHLKELLAGRGTEFADSGAVEDQPHPSVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS74</accession>
    <name>Q3SS74_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ETC complex I subunit conserved region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_1606</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1606"/>
    <dbReference type="EnsemblBacteria" id="ABA04867">
        <property type="protein sequence ID" value="ABA04867"/>
        <property type="gene ID" value="Nwi_1606"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1606"/>
    <dbReference type="eggNOG" id="ENOG5032RJS">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077196_4_1_5"/>
    <dbReference type="OMA" id="MIARIYS"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070469">
        <property type="term" value="C:respirasome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006885">
        <property type="entry name" value="NADH_UbQ_FeS_4_mit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038532">
        <property type="entry name" value="NDUFS4-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12219">
        <property type="entry name" value="PTHR12219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04800">
        <property type="entry name" value="ETC_C1_NDUFA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA04867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="115" mass="13403" checksum="E82535C21B1ADADF" modified="2005-10-11" version="1">MLPDQRQQQVPAKSMMARIFKPAKNAMQSGAARTREWQLDYEPEQPRAIEPLMGWTSSGDTKQQITLRFETREDAIAYCEREGIPYQVIEPKGRATRKAAYSDNFAFRRIEPWTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS93</accession>
    <name>Q3SS93_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Phosphate acetyl/butaryl transferase</fullName>
            <ecNumber evidence="11">1.1.1.40</ecNumber>
            <ecNumber evidence="11">2.3.1.8</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_1587</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the malic enzymes family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.40" evidence="11"/>
    <dbReference type="EC" id="2.3.1.8" evidence="11"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314854.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1587"/>
    <dbReference type="EnsemblBacteria" id="ABA04848">
        <property type="protein sequence ID" value="ABA04848"/>
        <property type="gene ID" value="Nwi_1587"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1587"/>
    <dbReference type="eggNOG" id="COG0280">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0281">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012366_0_0_5"/>
    <dbReference type="OMA" id="NIWVTDL"/>
    <dbReference type="OrthoDB" id="654531at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004471">
        <property type="term" value="F:malate dehydrogenase (decarboxylating) (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004473">
        <property type="term" value="F:malate dehydrogenase (decarboxylating) (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008948">
        <property type="term" value="F:oxaloacetate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008959">
        <property type="term" value="F:phosphate acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05311">
        <property type="entry name" value="NAD_bind_2_malic_enz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012301">
        <property type="entry name" value="Malic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037062">
        <property type="entry name" value="Malic_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012302">
        <property type="entry name" value="Malic_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045213">
        <property type="entry name" value="Malic_NAD-bd_bact_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012188">
        <property type="entry name" value="ME_PTA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042113">
        <property type="entry name" value="P_AcTrfase_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042112">
        <property type="entry name" value="P_AcTrfase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002505">
        <property type="entry name" value="PTA_PTB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01515">
        <property type="entry name" value="PTA_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036684">
        <property type="entry name" value="ME_PTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="8" id="KW-0521">NADP</keyword>
    <keyword evidence="4 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="malic" evidence="10">
        <location>
            <begin position="20"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Malic_M" evidence="9">
        <location>
            <begin position="165"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="78"/>
            <end position="85"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR036684-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR036684-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR036684-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA04848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="760" mass="81614" checksum="232A2849D6262A08" modified="2005-10-11" version="1">MSYSEDLRSAALSFHRSPRPGKLEIQPTKPLANQRDLALAYSPGVAAACEEIVADPAEAATLTGRANLVAVVSNGTAVLGLGNIGPLASKPVMEGKAILFKKFAGIDVFDIEIAADTIERVVETVAALEPTFGGINLEDIKGPECFEIEERLKGRMKIPVFHDDQHGTAIIVAAAVSNALMLNGKALAEVKIVASGAGAAAIACLNLLVSMGAQRKNIWVCDIDGVVYEGRDTLMDKWKAAYAQKTDKRKLAEVISGADVFLGVSAGGVLTPEMVAQMADRPLVLALANPVPEIMPEEARKARPDAMICTGRSDFPNQVNNVLCFPFIFRGALDVGATAINEEMKLAAVDAIARLAREPPLDAAIRFDSETQGFGPGSLIPGPFDPRLILRIAPAVAKAAMASGVATRPITNFDEYHARLERFAFRSGLVMKPVFAEAKKQPFRVIYAEGEDMRVLQATQQVLEEQLARPILVGRPSVVETRIRRLGLAIKAGRDFDLVNPEDDPRYRSYVQSYIDVAGRHGATPDVARTTIRTNATVIAALAVVRGEADAMLCGVEGSYMRHLRHIREIIGYLPETSDFAALSLMITGKGAYFIADTQVRPNPSAEELADIAGQAAVHVQRFNIKPKIAFVSHSDFGSYDTESARKARRATEIFITRHPDIEADGEMQGDTALSESARQLILPHSRLTGVANVLILPNLDAANAAYQMIKVLGNGLLVGPILIGPARPAHILTPSVTARGILNMTAIAVVEAQERAGRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSC3</accession>
    <name>Q3SSC3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF2312 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1557</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314824.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1557"/>
    <dbReference type="EnsemblBacteria" id="ABA04818">
        <property type="protein sequence ID" value="ABA04818"/>
        <property type="gene ID" value="Nwi_1557"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1557"/>
    <dbReference type="eggNOG" id="COG3750">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115384_0_0_5"/>
    <dbReference type="OMA" id="IFETYMA"/>
    <dbReference type="OrthoDB" id="1982771at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046367">
        <property type="entry name" value="GapR-like_DNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10073">
        <property type="entry name" value="DUF2312"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2312" evidence="2">
        <location>
            <begin position="8"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="206" mass="22687" checksum="44D0053773044F93" modified="2005-10-11" version="1">MTTARAEARSRLKDFIERIERIAADRRDLGDDMKAVFAEAKAEGFDTKAMRRIIKRRDKDPAEVAEEDSIFETYMAELGMTPEAPLPSMVRALGVDVLAREQVIDVLSSLIPNNGEIIARVGGDPMRLWRDEEGRAYAEVYVPPAAAPKEKTGRALKTPATVLTMVPGGKLATSDDVRRKADAAEKRSKVSKPDEDPDSDDEGEDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSK4</accession>
    <name>Q3SSK4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1476</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314744.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1476"/>
    <dbReference type="EnsemblBacteria" id="ABA04737">
        <property type="protein sequence ID" value="ABA04737"/>
        <property type="gene ID" value="Nwi_1476"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1476"/>
    <dbReference type="eggNOG" id="ENOG5031NKU">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126531_1_0_5"/>
    <dbReference type="OrthoDB" id="1910502at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021508">
        <property type="entry name" value="Gp17-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11367">
        <property type="entry name" value="DUF3168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="144" mass="15832" checksum="0EBEDCB10900B3CC" modified="2005-10-11" version="1">MSSPALALQKAIRLRLINTAAVTALVPPANILDRNERPAPSPSIILGEDQEVDEDFTLKRDYIRVYSMLHIWKTEPSTAGVKAIAGAVRKAIGRVRRLDIGDPDFVCADCRVDGVRYLRDPGGEMSHAVLTINSLVQARWSVVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSL0</accession>
    <name>Q3SSL0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1470</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1470"/>
    <dbReference type="EnsemblBacteria" id="ABA04731">
        <property type="protein sequence ID" value="ABA04731"/>
        <property type="gene ID" value="Nwi_1470"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1470"/>
    <dbReference type="eggNOG" id="ENOG50339EY">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2001458_0_0_5"/>
    <dbReference type="OMA" id="WFRELDC"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="124" mass="14042" checksum="ABDCDE68A13AC525" modified="2005-10-11" version="1">MINLQWLSDRLVYILRWRMDRHPGAENEPDAPEIPAAGLRVWGIFLDLHATRGSNGFGANPIAYADIESWSRLRREPVRPFELDMIRALDATWLEVAAALAKEPDKPKVSSRKVTPALFDAMFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSP9</accession>
    <name>Q3SSP9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Response regulator receiver</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1431</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314701.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1431"/>
    <dbReference type="EnsemblBacteria" id="ABA04692">
        <property type="protein sequence ID" value="ABA04692"/>
        <property type="gene ID" value="Nwi_1431"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1431"/>
    <dbReference type="eggNOG" id="COG0784">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_17_5"/>
    <dbReference type="OMA" id="RDYITKP"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="4"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="121" mass="13573" checksum="2D77608F45BAC06B" modified="2005-10-11" version="1">MAKTVLIVEDNELNMKLFRDLLEAHGYHTSGTSNGFEALDLVRKLRPDLVLMDIQLPQVSGLDVTRWIKDDPELRAIPVVAVTAFAMKGDEERIREGGCEAYLSKPISVGKFIETVRRFVG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSU1</accession>
    <name>RL17_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplQ</name>
        <name type="ordered locus">Nwi_1389</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Contacts protein L32.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314661.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSU1"/>
    <dbReference type="SMR" id="Q3SSU1"/>
    <dbReference type="STRING" id="323098.Nwi_1389"/>
    <dbReference type="EnsemblBacteria" id="ABA04650">
        <property type="protein sequence ID" value="ABA04650"/>
        <property type="gene ID" value="Nwi_1389"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1389"/>
    <dbReference type="eggNOG" id="COG0203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074407_2_0_5"/>
    <dbReference type="OMA" id="YAVTELF"/>
    <dbReference type="OrthoDB" id="1838961at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1030.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01368">
        <property type="entry name" value="Ribosomal_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000456">
        <property type="entry name" value="Ribosomal_L17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036373">
        <property type="entry name" value="Ribosomal_L17_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14413">
        <property type="entry name" value="PTHR14413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01196">
        <property type="entry name" value="Ribosomal_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64263">
        <property type="entry name" value="SSF64263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00059">
        <property type="entry name" value="L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01167">
        <property type="entry name" value="RIBOSOMAL_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000267901" description="50S ribosomal protein L17">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01368"/>
        </source>
    </evidence>
    <sequence length="138" mass="15549" checksum="BAD0A6A3C935C082" modified="2005-10-11" version="1">MRHGKVHRKLNRTAEHRKAMFANMSASLIKHEQIVTTLPKAKELRPIVEKLVTLGKKGGLAKRRQAISEMRDIDQVKKLFDVLAPRYKDRHGGYTRIIKAGFRYGDNAPMAVIEFVDRDVEAKGQDSGPVQNEASEAA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSW5</accession>
    <name>RL4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplD</name>
        <name type="ordered locus">Nwi_1365</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Forms part of the polypeptide exit tunnel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314642.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSW5"/>
    <dbReference type="SMR" id="Q3SSW5"/>
    <dbReference type="STRING" id="323098.Nwi_1365"/>
    <dbReference type="EnsemblBacteria" id="ABA04626">
        <property type="protein sequence ID" value="ABA04626"/>
        <property type="gene ID" value="Nwi_1365"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1365"/>
    <dbReference type="eggNOG" id="COG0088">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041575_5_2_5"/>
    <dbReference type="OMA" id="PQVHILE"/>
    <dbReference type="OrthoDB" id="1572673at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01328_B">
        <property type="entry name" value="Ribosomal_L4_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_L4/L1e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_L4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013005">
        <property type="entry name" value="Ribosomal_uL4/L1e"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10746">
        <property type="entry name" value="PTHR10746"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="SSF52166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03953">
        <property type="entry name" value="rplD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000242403" description="50S ribosomal protein L4">
        <location>
            <begin position="1"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01328"/>
        </source>
    </evidence>
    <sequence length="206" mass="22192" checksum="8B3B865129D13263" modified="2005-10-11" version="1">MELNVTTLEGKAAGSVHLSDGIFGLEPRKDLIQRCVNWQLAKSQAGTHKTKGRAEIWRTGKKLFKQKGTGNARHGSARAPQFRGGGRAFGPVVRSHAHDLPKKVRALALRHALSAKAKDGGLIVIDSVELKEAKTKALMGHFSSLGLTSALIIDGAQVHAGFATAARNIPNIDVLPIQGINVYDILRRQKLVLTKAAVDALEARFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST10</accession>
    <name>Q3ST10_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Rhomboid-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_1320</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1320"/>
    <dbReference type="EnsemblBacteria" id="ABA04581">
        <property type="protein sequence ID" value="ABA04581"/>
        <property type="gene ID" value="Nwi_1320"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1320"/>
    <dbReference type="eggNOG" id="COG0705">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055068_5_0_5"/>
    <dbReference type="OMA" id="VPVFNMP"/>
    <dbReference type="OrthoDB" id="2053476at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="SSF144091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="197"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Rhomboid" evidence="6">
        <location>
            <begin position="78"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="264" mass="27980" checksum="D63713F69C3C31CA" modified="2005-10-11" version="1">MDSPPDPSSERPPEPPREPILTLPGPLTAYIGLLAAIHVMWSSVPPDLEYWIIEAFGFIPKRYDPSLLATAFPGGSGAKVWTFVTYSLLHANLSHIGFNILWLLPFGSALARRFGGFRFFLFMAVAAVAGAAAHLVTHEHSIAPMIGASGSVSGAMAASIRFAFAQGSFLSFGRGDADAAAQVPALSLTRALQNPRVLAFLAVWFGINIIFGMGSIAIGSEPGSVAWQAHMGGFMAGLLLFSLFDPVPRPARDPDDASSGADHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST12</accession>
    <name>Q3ST12_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1318</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314597.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1318"/>
    <dbReference type="EnsemblBacteria" id="ABA04579">
        <property type="protein sequence ID" value="ABA04579"/>
        <property type="gene ID" value="Nwi_1318"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1318"/>
    <dbReference type="eggNOG" id="COG5388">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097079_1_0_5"/>
    <dbReference type="OMA" id="MKHPTSR"/>
    <dbReference type="OrthoDB" id="1397399at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009922">
        <property type="entry name" value="DUF1457"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07310">
        <property type="entry name" value="PAS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031878">
        <property type="entry name" value="UCP031878"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="185" mass="20929" checksum="10A9C03B064D8CC7" modified="2005-10-11" version="1">MKHPSNRAFFAYWDEKRDGERAPDRRVIEPNAVRQLLGDIFVLSYDPERGYPFRVAGTRVCALLDRDLKGDNFSALFAAASRDEITDILGIVSEELLVSVAGLTATERNGSLAHFELLLLPFNHRAHTPISLTGLLAPMQHSRQPVADFTLTSWRYLNHPPQRFVPRALKKLAIARGFMVYEGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST71</accession>
    <name>Q3ST71_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Virulence-associated protein E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_1259</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314548.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1259"/>
    <dbReference type="EnsemblBacteria" id="ABA04520">
        <property type="protein sequence ID" value="ABA04520"/>
        <property type="gene ID" value="Nwi_1259"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1259"/>
    <dbReference type="eggNOG" id="COG0358">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059689_2_1_5"/>
    <dbReference type="OMA" id="LWQSATA"/>
    <dbReference type="OrthoDB" id="1071997at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01029">
        <property type="entry name" value="TOPRIM_primases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036977">
        <property type="entry name" value="DNA_primase_Znf_CHC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034154">
        <property type="entry name" value="TOPRIM_DnaG/twinkle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13362">
        <property type="entry name" value="Toprim_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="1" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2" id="KW-0639">Primosome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Toprim" evidence="7">
        <location>
            <begin position="200"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA04520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="296" mass="31864" checksum="6D446BAC7EDE2240" modified="2005-10-11" version="1">MTAEIIARALGGRKVGQCWTARCPAHDDRVPSLSIRDDGGKVLVRCHAGCEQRDVIAILKERGLWDGRQPSTVRKSHRRMVLPQRPDPHQAERSAYALSIWQSALPADGTPVEVYLASRGLTCSLPPSLRFHVGLKHPSGGIFPCMVALVTRGADAMPMAIHRTFLAREGRGKAQVEPTKMMLGPCQGGAVRLGDPGEILMIGEGIETCLAAMQATGHPVWAALSTSGLRALDLPGSVRDVIVLADGDDPGEAAARACASRWMRQGRRARIARPPRGLDFNDVLLGRVSDIEGGRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST87</accession>
    <name>Q3ST87_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Lipid A biosynthesis acyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_1242</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314533.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1242"/>
    <dbReference type="EnsemblBacteria" id="ABA04504">
        <property type="protein sequence ID" value="ABA04504"/>
        <property type="gene ID" value="Nwi_1242"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1242"/>
    <dbReference type="eggNOG" id="COG1560">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049421_4_2_5"/>
    <dbReference type="OMA" id="VFGDMCD"/>
    <dbReference type="OrthoDB" id="1106624at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009247">
        <property type="term" value="P:glycolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd07984">
        <property type="entry name" value="LPLAT_LABLAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004960">
        <property type="entry name" value="LipA_acyltrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30606">
        <property type="entry name" value="PTHR30606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03279">
        <property type="entry name" value="Lip_A_acyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA04504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="310" mass="34841" checksum="CBF9D15DE12E6E9C" modified="2005-10-11" version="1">MRRLLLRTRAGLRDAVKPVGAAAVGALTIALLRATRCFDPIKTADLFGRITRKIGPLLREQKIGRANLTAAFPEKPPDEIEKILAGVWDNLGRIGAEFAHLDHVWNHDPDRPEDGRIEIGERTRELFHKLRLDGKPALIFASHIGNWELPAVAAVAHGLDAAILYRRPNIASADRAIKQLRSVNMGTLIPAGRDAPLRLAQALQNGQHVAMLVDQYFTNGVDVTFFGRKTKANPTLARLLRQIECPIHGVRIIRLPDHRFRAELSEEITPARDANGGIDVQGTMQIITSVIEGWIREYPDQWLWLHRRWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STD9</accession>
    <name>Q3STD9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1190</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314481.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1190"/>
    <dbReference type="EnsemblBacteria" id="ABA04452">
        <property type="protein sequence ID" value="ABA04452"/>
        <property type="gene ID" value="Nwi_1190"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1190"/>
    <dbReference type="eggNOG" id="ENOG502Z9X1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_585098_0_0_5"/>
    <dbReference type="OMA" id="QPWQIVR"/>
    <dbReference type="OrthoDB" id="781597at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="463" mass="51081" checksum="957EBB3BFFD609C9" modified="2005-10-11" version="1">MDQTSIERLKDYLAQLPPKSQALLMREFERAVERGEDAAVANLVLGELRRIDPQSGDSEQRSEDPVRHLFRPLDPFLVEDNVVPRSGQIRRNSLVPVWRWLAREGAPDDARAFEAVLAAEAGSASGIETAARVFQPVAAAAILRILTDSSGINRQRTLGRISAPGVIEDLPAIAAVLHAREALDTLNIRLPGFLPVFGEPQIASLMTALNVPSLQTPAALPFALSLVMQRLSAPWQIIRLAVQVAGSDDEIRVAATPYGVAVKMALDDLSQTSAVLRADIRRGRFENVSRHLKTLHDGVRGLRTELDIRTDSGWGRQLAAIRVDISEVLQSEIEGVAGRVRRILRQRPDKDIPAGAKLDSIEVEETAALIDFVAVCRTYASELAINEVTTRTYSDLQHYVERTSETLVEALRNGDERVRAFRQQQVKAVIRFCEVLFGRDYSSLMSRAADHALTAERKSSRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STE4</accession>
    <name>Q3STE4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1185</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314476.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1185"/>
    <dbReference type="EnsemblBacteria" id="ABA04447">
        <property type="protein sequence ID" value="ABA04447"/>
        <property type="gene ID" value="Nwi_1185"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1185"/>
    <dbReference type="eggNOG" id="ENOG5030YD1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1935817_0_0_5"/>
    <dbReference type="OMA" id="QKAYPSV"/>
    <dbReference type="OrthoDB" id="2033197at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229106" evidence="1">
        <location>
            <begin position="18"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="130" mass="13340" checksum="0A171789E7AEA2C0" modified="2005-10-11" version="1">MRKLVAAIAVVCGLALAGCQTVALPSLNLNSAASLNTVYGIQNAFGVAVNAANAYRALPLCRTGTGPGMINICAKRSVIERLQSAMRKARLAVNNLVALQKAYPSVDISNAFAAARSALIAVQQILASGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STF0</accession>
    <name>Q3STF0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1179</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314470.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1179"/>
    <dbReference type="EnsemblBacteria" id="ABA04441">
        <property type="protein sequence ID" value="ABA04441"/>
        <property type="gene ID" value="Nwi_1179"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1179"/>
    <dbReference type="eggNOG" id="COG5449">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080134_0_0_5"/>
    <dbReference type="OMA" id="HMPGNDF"/>
    <dbReference type="OrthoDB" id="756610at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011928">
        <property type="entry name" value="Phage_phiJL001_Gp84"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018964">
        <property type="entry name" value="Phage_phiJL001_Gp84_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019228">
        <property type="entry name" value="Phage_phiJL001_Gp84_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09931">
        <property type="entry name" value="DUF2163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09356">
        <property type="entry name" value="Phage_BR0599"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02218">
        <property type="entry name" value="phg_TIGR02218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2163" evidence="2">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Phage_BR0599" evidence="1">
        <location>
            <begin position="195"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="297" mass="30971" checksum="6ACA205AFD383BD7" modified="2005-10-11" version="1">MRSIPSALQAKLDSGATTLARCWIVTRRDGVVLGFTDHDRDLTIGGVLCRAATGFTASEATSRFDLAVDGGEISGAFSDDSLSEADLAAGRYDAAGVATWLVDWSDVSLRILIARGTIGEVRREGAAFTAELRGLADALAQDSGRLFTARCGVDLGDGKCRVDLASSAFRRSGTVTAVEGTSIVAVSGLSGLAAGWFTAGRLNWTGGANAGLAIEVKQHRVVAGVVRLSLWRAMAEPIAIGDSFVVTAGCDKSFTTCRDRFANTSNFRGFPQIPGNDFVVSYPLPGSPDNGNGSKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STK3</accession>
    <name>Q3STK3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1126</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314419.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1126"/>
    <dbReference type="EnsemblBacteria" id="ABA04388">
        <property type="protein sequence ID" value="ABA04388"/>
        <property type="gene ID" value="Nwi_1126"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1126"/>
    <dbReference type="eggNOG" id="COG3334">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075493_1_0_5"/>
    <dbReference type="OMA" id="FDRLEMP"/>
    <dbReference type="OrthoDB" id="1992240at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="56"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="244"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="122"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="267" mass="28690" checksum="41F52E2F7AACFB52" modified="2005-10-11" version="1">MSAFRDIRVIPVVMVAVFGLAVLKVAGLVIDGGYVFDVQPASAGQSWAQENLNFPGGTPDAADITGSVEAKKGDAGKDDAAKDEPKNEESRPAVGTPGTAKAGAIILPEPGQTVSPSERAILERLQSRREELEARAREIDIRESLLRAAEQRIQGRVEQMKGIEARIATATAEKNEAEIARFKGLVTMYENMKPKEAAKVFDRLEMPVLFEIASKIAPRKMSDILGQMSPEAAERLTVELARQASGGATSASNLPKIEGRLTPQGRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STK9</accession>
    <name>Q3STK9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Flagellar protein FlgA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_1120</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1120"/>
    <dbReference type="EnsemblBacteria" id="ABA04382">
        <property type="protein sequence ID" value="ABA04382"/>
        <property type="gene ID" value="Nwi_1120"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1120"/>
    <dbReference type="eggNOG" id="COG1261">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061553_1_0_5"/>
    <dbReference type="OMA" id="DITYDPR"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044780">
        <property type="term" value="P:bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017585">
        <property type="entry name" value="Flag_basal_body_FlgA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039246">
        <property type="entry name" value="Flagellar_FlgA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013974">
        <property type="entry name" value="SAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36307">
        <property type="entry name" value="PTHR36307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13144">
        <property type="entry name" value="ChapFlgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00858">
        <property type="entry name" value="SAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03170">
        <property type="entry name" value="flgA_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0282">Flagellum</keyword>
    <keyword evidence="3" id="KW-0574">Periplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="SAF" evidence="4">
        <location>
            <begin position="221"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="380" mass="39870" checksum="CC99125266C53111" modified="2005-10-11" version="1">MPGKKEEKLIMAVRPLLVAVVAGALLAAPAPVLAGNDAAARRADLASAFRTAAAIPAPVLRGRVDVTDKMVRIGDLVDNAGDAAQIAIYRAPDPGTTGTLPAAQLLDVLRAHQVIGVETHGIGNVAVTRLARTLDPDDVELQIAVALEHRNGLGDARDLLLTFDKDLKAIHLDAANNGAVTPLVTRFDPRSGRFDVTLEVGNDSGAAPTRLRFTGTAVETVEAAVLARDVDRNTLLKSSDIVIERRPRAEVGNNPASRDHVVGMQTRRQMRAGQALHGIDLTRPDLVQRDQAVTLIYQTAGIYLTVRGKATESGTEGDVVSVLNLQSNRTISGVVVGRGQVSVSPAAPVVPHRLASVEPAVETTASFSAGSNAPASSKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STN5</accession>
    <name>Q3STN5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphoglycerate/bisphosphoglycerate mutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1094</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1094"/>
    <dbReference type="EnsemblBacteria" id="ABA04356">
        <property type="protein sequence ID" value="ABA04356"/>
        <property type="gene ID" value="Nwi_1094"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1094"/>
    <dbReference type="eggNOG" id="COG2062">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084603_2_3_5"/>
    <dbReference type="OMA" id="DRPLNKR"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="SSF53254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="183" mass="19865" checksum="942747B7D58D3296" modified="2005-10-11" version="1">MNRVSVMRRLLLLRHAKTERDSPSGSDRDRPLAARGRNDAAELGLWLAGDPSLRPDLVLISTAVRARETWDIVRQQFSDVTAQGPAEHLPELYGAEAVQLLAIIRSLGTDHERLMIVGHNPGLHELALRLIGGGDAKGRAALADNLPTSGLAVIDFAVENWECAAFGSGQLKRLVSPKLLKQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STN9</accession>
    <name>Q3STN9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314386.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04352">
        <property type="protein sequence ID" value="ABA04352"/>
        <property type="gene ID" value="Nwi_1090"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1090"/>
    <dbReference type="eggNOG" id="ENOG5030QXU">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017225_0_0_5"/>
    <dbReference type="OMA" id="SQRAREC"/>
    <dbReference type="OrthoDB" id="110951at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="674" mass="71588" checksum="AC3E6B65D81DDF2F" modified="2005-10-11" version="1">MARAHVSSVIGIVLPLLCTLAVGSLAVGSLAAQQIGEQPLSDGIAEAGPAAPIPDRAAGGMQISWEVRNRFRLFREERDFQIHAAAESNQSILAAEQAFERQSDGRGWARNAVNRLCIDLGGHVSEPCTRDNVKESYLTPTEHPVTVRLTGAVPVGAVCAWSFDDGDSPRGTTQDCTEPIDFRARYGRATSATVDVTSGDATQRLTAQVKVRDILIAGLGDSIASGEGNPDRAVPLSDEGFCFRSYLGGAGREFYRPSRAGYKGGRACGTSGSLQSWQQFGALWMNAACHRSLYSYQTRAALALAVRYPHVAVTYLPLACTGASIPEGILGSQRARECLTAKSSAKCQGTVNGQIAQLRDALAAARHRQPERKLDLVLMTIGANDINFSGLVSDVIVDAATERALFRRGGLIGSVDGSRSSLERDLPQAFTRLRAALRPLLDNDLSRVVFVSYANPALANGGAPCPGGKAGFDVHPSFNADSGRLTAVTEYVRNEFLPGLKALASCQSGVICRDPAGDRMTFVDAHQSAFANHGFCARADSDPEFDRECFSAKGDSFVGSIVEAPHQPLVCGRGASAFRAYLPRARWIRDANDSYFTAMTYPQAQSSLMQPSDIHDATWGLLSAVYGGAVHPTAEGHAAMADAAMPAIASQLHLNAADAGDVTGEPLPPPTPQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STZ2</accession>
    <name>Q3STZ2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0987</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0987"/>
    <dbReference type="EnsemblBacteria" id="ABA04249">
        <property type="protein sequence ID" value="ABA04249"/>
        <property type="gene ID" value="Nwi_0987"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0987"/>
    <dbReference type="eggNOG" id="COG5480">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120931_0_0_5"/>
    <dbReference type="OMA" id="EGWWNVA"/>
    <dbReference type="OrthoDB" id="1606669at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009380">
        <property type="entry name" value="DUF1036"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06282">
        <property type="entry name" value="DUF1036"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="194" mass="20936" checksum="3DE626F4CA4CFE34" modified="2005-10-11" version="1">MTKPDHLLFLTLSRRASIAGFLPALGLVVVCLWSAPAAADFRLCNNTSSRVGIALGYKSADGWTTEGWWNISSHACETLLRGTLIARYYYIYALDYDHGGEWSGQAFMCSRDKEFTIKGTENCLARGFDRTGFFEIDTGDQRAWTVQLTESGEHGPQRVPGMPGQDGIPGGSDSTGGTPPGSGLTPDALPGRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU47</accession>
    <name>Q3SU47_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0932</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0932"/>
    <dbReference type="EnsemblBacteria" id="ABA04194">
        <property type="protein sequence ID" value="ABA04194"/>
        <property type="gene ID" value="Nwi_0932"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0932"/>
    <dbReference type="eggNOG" id="COG5338">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030554_0_0_5"/>
    <dbReference type="OMA" id="EVDHDFR"/>
    <dbReference type="OrthoDB" id="950266at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018759">
        <property type="entry name" value="BBP2_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10082">
        <property type="entry name" value="BBP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229126" evidence="2">
        <location>
            <begin position="34"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="563" mass="60874" checksum="966E04AD98DECD13" modified="2005-10-11" version="1">MSSGPATGFCRSVPIWRAVLACLAFIGPGCATAGAQTLSGDQITPLRGGLFQPRESSLRRSGEPAGDVASVSSTMAPSRIGRTPTYDIPAATGAADTGYDSLNRRRKEKKLYPGAPKPKQPPGPGNFVPVPPPPPLSVPPSSTAHTTPIAPAMAGRVEGQPARRRLKRDDDPFGAAGNYVGSFFVKSAVELWGGYNTNPGRFNQPKGSAFYMVAPEFVAASDWTRHSLLVDLRGSFTGFDRTFPLGADNPPQSVISVPTVVDRPDFLGRAVGRLDVRRDTRINSELRMRIGIDNPGSPNVQAGLARYPIFAVTGGTAGIEHDINRLQVSLHGFADRTMYQNSLLTDGTSASNADRNFNQYGAVGRVSYDLLPGFKPFGEVEADARNRDTRVDRDGFQRDSHGGYAKVGTSFELTRLLIGEASIGYTARNYADPRLDQLTGLLTSGSLIWSASALTTARFTSVTFVDESFVPGVAGVLTRSHTIEIDHDFRRWLTGIGRFTYATQEYRGSERSDQIYSISGDLIYRLNREFQIKAQVRHDVLSTNIPGANSAATVVMLGVRLQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUD5</accession>
    <name>Q3SUD5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ISBm1, transposase orfA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0309</name>
        <name evidence="2" type="ordered locus">Nwi_0842</name>
        <name evidence="3" type="ordered locus">Nwi_1096</name>
        <name evidence="4" type="ordered locus">Nwi_1292</name>
        <name evidence="5" type="ordered locus">Nwi_1297</name>
        <name evidence="6" type="ordered locus">Nwi_2224</name>
        <name evidence="7" type="ordered locus">Nwi_2412</name>
    </gene>
    <organism evidence="2 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="2 8" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="2">Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0309"/>
    <dbReference type="EnsemblBacteria" id="ABA03576">
        <property type="protein sequence ID" value="ABA03576"/>
        <property type="gene ID" value="Nwi_0309"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04106">
        <property type="protein sequence ID" value="ABA04106"/>
        <property type="gene ID" value="Nwi_0842"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04358">
        <property type="protein sequence ID" value="ABA04358"/>
        <property type="gene ID" value="Nwi_1096"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04553">
        <property type="protein sequence ID" value="ABA04553"/>
        <property type="gene ID" value="Nwi_1292"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04558">
        <property type="protein sequence ID" value="ABA04558"/>
        <property type="gene ID" value="Nwi_1297"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05478">
        <property type="protein sequence ID" value="ABA05478"/>
        <property type="gene ID" value="Nwi_2224"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05666">
        <property type="protein sequence ID" value="ABA05666"/>
        <property type="gene ID" value="Nwi_2412"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0309"/>
    <dbReference type="KEGG" id="nwi:Nwi_0842"/>
    <dbReference type="KEGG" id="nwi:Nwi_1096"/>
    <dbReference type="KEGG" id="nwi:Nwi_1292"/>
    <dbReference type="KEGG" id="nwi:Nwi_1297"/>
    <dbReference type="KEGG" id="nwi:Nwi_2224"/>
    <dbReference type="KEGG" id="nwi:Nwi_2412"/>
    <dbReference type="eggNOG" id="COG3293">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055261_2_2_5"/>
    <dbReference type="OMA" id="PDRYGPY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025161">
        <property type="entry name" value="Put_IS4/IS5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13340">
        <property type="entry name" value="DUF4096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="121" mass="13827" checksum="B78179F90D9B6447" modified="2005-10-11" version="1">MARFDLTDFEWSVIQPLLPNKSRGVARVDDRRVLNGIFWRLRTGAPWADIPARYGPHTTCVNRFNRWRKAGVWDRILSAVSKAYDGDIQMIDSSSIRVHQHAANGQKNRRDPVAWVARAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUJ6</accession>
    <name>Q3SUJ6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">C4-dicarboxylate transporter/malic acid transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_0780</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0780"/>
    <dbReference type="EnsemblBacteria" id="ABA04045">
        <property type="protein sequence ID" value="ABA04045"/>
        <property type="gene ID" value="Nwi_0780"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0780"/>
    <dbReference type="eggNOG" id="COG1275">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052472_0_0_5"/>
    <dbReference type="OMA" id="LSPPYWI"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004695">
        <property type="entry name" value="SLAC1/Mae1/Ssu1/TehA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038665">
        <property type="entry name" value="Voltage-dep_anion_channel_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03595">
        <property type="entry name" value="SLAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="221"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="259"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="294"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="326"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA04045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="357" mass="40184" checksum="D1412E39B6EE6939" modified="2005-10-11" version="1">MQPIDSSKRRFRLSMPGARRLSPAYFALVMATGIVSIASEKAGFHAIAVALLWLNATQFVVLWILTSWRLIRYRRALIVDLTDHRSAPGFLSAVAATGVMANQFILAGALDIALGLWIFGILLWVILTYTIFANLTIMEAKPTIDQGITGAWLLAVVSTQSISLLSALLARHIHQPLRLDVNFLALSMLLWGGMLYISIISLIFYRYMFFKFSPADFVPSYWINMGAMAISTLAGSLLIENAADAWFLEELLPFLKGFTIFFWAAGTWWIPLLIILFVWRHFYRRFPLRYDPAYWSAVFPLGMYTVCTYEMAGAMRLEFLRPIADVFVYVALLAWTAAFIGLVKKLAGNLIPAPSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUK9</accession>
    <name>Q3SUK9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0767</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314081.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0767"/>
    <dbReference type="EnsemblBacteria" id="ABA04032">
        <property type="protein sequence ID" value="ABA04032"/>
        <property type="gene ID" value="Nwi_0767"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0767"/>
    <dbReference type="eggNOG" id="COG5349">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133751_0_0_5"/>
    <dbReference type="OMA" id="IALQFHH"/>
    <dbReference type="OrthoDB" id="1899826at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009325">
        <property type="entry name" value="DUF983"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06170">
        <property type="entry name" value="DUF983"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="130" mass="14352" checksum="D188F721F1A09900" modified="2005-10-11" version="1">MSNFLVRPTLSQTILRGIACKCPRCGKGKLFSGFLTLRPACDRCEQDYSFIDAGDGPAVFIILIAGFIVVAAALVVEVKYQPALWIHAVLWIPLVLAVTLWPLRAMKSLLISLQFHHKAAEGRAVPRRHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUT4</accession>
    <name>Q3SUT4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pyrroloquinoline-quinone synthase</fullName>
            <ecNumber evidence="2">1.3.3.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Coenzyme PQQ synthesis protein C</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Pyrroloquinoline quinone biosynthesis protein C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pqqC</name>
        <name evidence="4" type="ordered locus">Nwi_0691</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Ring cyclization and eight-electron oxidation of 3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic-acid to PQQ.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>6-(2-amino-2-carboxyethyl)-7,8-dioxo-1,2,3,4,7,8-hexahydroquinoline-2,4-dicarboxylate + 3 O2 = H(+) + 2 H2O + 2 H2O2 + pyrroloquinoline quinone</text>
            <dbReference type="Rhea" id="RHEA:10692"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:58442"/>
            <dbReference type="ChEBI" id="CHEBI:58778"/>
            <dbReference type="EC" id="1.3.3.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; pyrroloquinoline quinone biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PqqC family.</text>
    </comment>
    <dbReference type="EC" id="1.3.3.11" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0691"/>
    <dbReference type="EnsemblBacteria" id="ABA03957">
        <property type="protein sequence ID" value="ABA03957"/>
        <property type="gene ID" value="Nwi_0691"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0691"/>
    <dbReference type="eggNOG" id="COG5424">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080136_0_0_5"/>
    <dbReference type="OMA" id="AYVHFVR"/>
    <dbReference type="OrthoDB" id="1571811at2"/>
    <dbReference type="UniPathway" id="UPA00539"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033732">
        <property type="term" value="F:pyrroloquinoline-quinone synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006725">
        <property type="term" value="P:cellular aromatic compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018189">
        <property type="term" value="P:pyrroloquinoline quinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.910.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00654">
        <property type="entry name" value="PQQ_syn_PqqC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016084">
        <property type="entry name" value="Haem_Oase-like_multi-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011845">
        <property type="entry name" value="PqqC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039068">
        <property type="entry name" value="PqqC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004305">
        <property type="entry name" value="Thiaminase-2/PQQC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40279">
        <property type="entry name" value="PTHR40279"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03070">
        <property type="entry name" value="TENA_THI-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48613">
        <property type="entry name" value="SSF48613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02111">
        <property type="entry name" value="PQQ_syn_pqqC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1 2" id="KW-0884">PQQ biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TENA_THI-4" evidence="3">
        <location>
            <begin position="25"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="283" mass="31661" checksum="892DC51789644945" modified="2005-10-11" version="1">MVSKSAATAPRFPLHPAPDRNAFEARLRAIGAERYHDKHVFHRMLHGGQCTLTQARAWVINRYYYQSRIPMKDAAFMSRVDDPGLRRAWRRRIEEHDGGLQEGGGLRRWLKLAEATGLDPDYVASTRGVLPGTRFAVDAYVSFVREKPMLEAVASSLTEMFARAIHEDRIAGLLEHYAFANDNALSYFRQRLKEAPREVEFGLSYVLDNADTMEKQDAAAAALIFKTDVLWSQLDALHFAYVAPGFIPPGAWNGRDGVGADEGEIAQAERTSGHDTEHCEAGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUV0</accession>
    <name>Q3SUV0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cyclic nucleotide-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0675</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0675"/>
    <dbReference type="EnsemblBacteria" id="ABA03941">
        <property type="protein sequence ID" value="ABA03941"/>
        <property type="gene ID" value="Nwi_0675"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0675"/>
    <dbReference type="eggNOG" id="COG0664">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075053_3_2_5"/>
    <dbReference type="OMA" id="CHCEGCP"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012318">
        <property type="entry name" value="HTH_CRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13545">
        <property type="entry name" value="HTH_Crp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00419">
        <property type="entry name" value="HTH_CRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="SSF51206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51063">
        <property type="entry name" value="HTH_CRP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="24"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="HTH crp-type" evidence="3">
        <location>
            <begin position="158"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="240" mass="26540" checksum="96C251618F0E4583" modified="2005-10-11" version="1">MNAAARSALFMSETIESLNVGARFLDRLSVEQRAQVRAAGRGLVVRQGDPVFSQGEHHDGIFIIDRGQVRVYYSAPSGREITLAYWTPGHFIGGPGVSGGGVYMWSGVAIEDCAITMLPSAVLRKLLLQMPDFALAMIDGLIAKGECYSSMAQMLGTRSVIERLAQYLVNLSELYGIDGGDAIIINRKVTHDQIAAMVGSTRQWVTMMLKRFQNEQIIAIDGNIMRIKRLDMLKKILFRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUW7</accession>
    <name>Q3SUW7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Secretin/TonB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0658</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313982.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0658"/>
    <dbReference type="EnsemblBacteria" id="ABA03924">
        <property type="protein sequence ID" value="ABA03924"/>
        <property type="gene ID" value="Nwi_0658"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0658"/>
    <dbReference type="eggNOG" id="COG4773">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008287_21_0_5"/>
    <dbReference type="OMA" id="SHSWART"/>
    <dbReference type="OrthoDB" id="439303at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012910">
        <property type="entry name" value="Plug_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037066">
        <property type="entry name" value="Plug_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011662">
        <property type="entry name" value="Secretin/TonB_short_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039423">
        <property type="entry name" value="TonB-dep_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036942">
        <property type="entry name" value="TonB_rcpt_b-brl_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32552">
        <property type="entry name" value="PTHR32552"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07715">
        <property type="entry name" value="Plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00965">
        <property type="entry name" value="STN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="STN" evidence="6">
        <location>
            <begin position="79"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="507" mass="54405" checksum="AB94061798CED62A" modified="2005-10-11" version="1">MRQGDLNGGSGRGERVIRGRRYLSFLLSTAMIGFTGMLGVAPACAQQTRQAVTSQPVNFSIPAQPLSSAINAFIRATGWQISYSSQLVHGKTSVAVVGAMPPSAALQRLVSGTGLSVRMGGSGSAALVGAAGAADAAVAPVAGAISLDTIDVQGATSSDPGRTEGTGSYTTSVASFGKNQTLKETPQTVSVITRQRIQDQGMTTMEHALERTPGITVVPYSTTDSTIYSRGFPVTNLQIDGNSPQFGGSMIGQGFTTSQFDLAMFDRVEVLRGSDALYGTSGAPGGAINLVRKKPTKQFQMNALLHGGSWNNYRGELDVGGSLTENGAVRGRFVGTYQDQNFFYNHGKSNRYLLYGIIEADITDSTMLTFGANVMRQDQAGFFRGLPRYANGDDLGLPRSFSLNRPEDTWLLNNNMMFIRLDQELAPNWTLGVEATRSINEIFRRDLTWQGGINPITGMGLTGYDWEYDYRAPQFTLDAVLKGSFDLLGREQKLTLGVSAVRALETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV66</accession>
    <name>Q3SV66_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PepSY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0558</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313886.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03825">
        <property type="protein sequence ID" value="ABA03825"/>
        <property type="gene ID" value="Nwi_0558"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0558"/>
    <dbReference type="eggNOG" id="ENOG5033N06">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2524151_0_0_5"/>
    <dbReference type="OMA" id="RYMEVDV"/>
    <dbReference type="OrthoDB" id="1845352at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025711">
        <property type="entry name" value="PepSY"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13670">
        <property type="entry name" value="PepSY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229244" description="PepSY domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="PepSY" evidence="2">
        <location>
            <begin position="6"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="84" mass="8746" checksum="A2DC1B6851DBECE5" modified="2005-10-11" version="1">MRKYILALAAAISLGAAAPAVAYDSGSLLSMHDALDVALNAGLISVSDAEFAGYEWQIDGRDAAGRYMEVDVDATTGAVLNINR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV92</accession>
    <name>Q3SV92_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Plasmid segregation oscillating ATPase ParF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0532</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0532"/>
    <dbReference type="EnsemblBacteria" id="ABA03799">
        <property type="protein sequence ID" value="ABA03799"/>
        <property type="gene ID" value="Nwi_0532"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0532"/>
    <dbReference type="eggNOG" id="COG1192">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037612_5_3_5"/>
    <dbReference type="OMA" id="ADHIAKP"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CbiA" evidence="1">
        <location>
            <begin position="26"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="234" mass="25475" checksum="6F26863355559E5F" modified="2005-10-11" version="1">MLLPDNVRICYRGAGMSSPRLRRRIITVTQRKGGVGKTTIAICVAAALARRGHEVALVDSDPQRSASQWAEPGNLEFPVYEMALEQTSVPAWAQEVRSIEAGIVVIDTAPNAREMGASIAVANLILVPCTPSGLDLDATLQTLAIIDAARERRRDRIKVILVPNRLDRRTLEGRQLLDELSTFGEAVGPPIASRAAFVRCFTSGQSVTSFMPGDTADQEIQELADAIERIELEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVD2</accession>
    <name>Q3SVD2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha/beta hydrolase fold protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0492</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313820.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0492"/>
    <dbReference type="ESTHER" id="nitwn-q3svd2">
        <property type="family name" value="AHL-acylase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03759">
        <property type="protein sequence ID" value="ABA03759"/>
        <property type="gene ID" value="Nwi_0492"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0492"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_50_2_5"/>
    <dbReference type="OMA" id="YSMEGYA"/>
    <dbReference type="OrthoDB" id="1282004at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="25"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="286" mass="30902" checksum="1E6373FEBD044D04" modified="2005-10-11" version="1">MLSRTIETSQGRLAFLESSGSGTPVVFIHGNSTCRDVFRHQFETTLAATQRIIAIDLPGHGQSDDALDPQKAYTIPGYAATLIEALRSLNITRAALVGWSLGGHVSLEMIGQGFDAAGAMIIGAPPIKRGILGMVRGFQAQLDLLLATKSALNARDISRFARACVGEKHAAQLHDTIARTDRRARQILGRGLRSGLGVDQRWVVENLPTPIAVVNGSGEPFARLDYVAGISYAALWDHRCHIIDGVGHAPFLEAPERFNPLLLRFLGDIHCSRIDGTTQPQIRSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVF0</accession>
    <name>Q3SVF0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">HemY protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0474</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313805.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0474"/>
    <dbReference type="EnsemblBacteria" id="ABA03741">
        <property type="protein sequence ID" value="ABA03741"/>
        <property type="gene ID" value="Nwi_0474"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0474"/>
    <dbReference type="eggNOG" id="COG3898">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028454_0_0_5"/>
    <dbReference type="OMA" id="ERWAPVS"/>
    <dbReference type="OrthoDB" id="1425742at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010817">
        <property type="entry name" value="HemY_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016982">
        <property type="entry name" value="Mms48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07219">
        <property type="entry name" value="HemY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031802">
        <property type="entry name" value="UCP031802"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="41"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="HemY_N" evidence="6">
        <location>
            <begin position="26"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="458"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="476"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="518"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="555" mass="59955" checksum="0CE9A39653D9F3E7" modified="2005-10-11" version="1">MLRIILFLVLIALSALGATWIADQTGTIVLSWDVWRIETTIPVFALGLGLLIVASLLAWSVVHGLWQAPTRMRRARRERRIARGRDAITRGLLAIGYGDAAAARNHAKAARRLVSNDPLALLLHAQAAQLDGDADRAQRAFRTMAERNDTRLLGLRGLFIEAQRAGDPAAAVAIAEEALKTSPSSIWASQAALGFRCARGDWTGALAILDKNVASGRIDKALYRRQRGVLLTARALELEKSDRDLSRQTVMEAVKFAPTLIPAVVLASKYLSEANQMRRAMRLIETAWLAQPHPDLADAYAHVMPGDSALQRLARVEKLSEKAPGHPESAMAIARAAIDASEFARAREVLEPLIAAPTQRVAMLMAEIEHNERGDSGRARAWTLRAVRALHDPVWTADGYVSDHWRPVSPLTGRVDAFQWQTPLSALPSSRPPLVEAEVSDQIAEDAPRVEALPAATSTEDFKAQDGAIPPAGVEASSSGTISMPDETATASADYTPSEEDNKPGAIPDVIPIVRAPDDPGVDDDNPRNDGFVEDDTAAARQAGGLRGLLSRRGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVI6</accession>
    <name>Q3SVI6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0438</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313769.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0438"/>
    <dbReference type="EnsemblBacteria" id="ABA03705">
        <property type="protein sequence ID" value="ABA03705"/>
        <property type="gene ID" value="Nwi_0438"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0438"/>
    <dbReference type="eggNOG" id="COG1940">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_737380_0_0_5"/>
    <dbReference type="OMA" id="NWESSRF"/>
    <dbReference type="OrthoDB" id="1193463at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000600">
        <property type="entry name" value="ROK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18964">
        <property type="entry name" value="PTHR18964"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00480">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="344"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="348"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="375" mass="41041" checksum="77FAA8ED6E9895C7" modified="2005-10-11" version="1">MAEDVLTTSGIGKHGVLRLPSVDIDNYNIELKDDDGFLGDRACKGAFRRILDDLRAPLKKNGEDPLGKTPSEAIAKGSLDEALVGEDIEASALVHGAIEEFAQELAYVTGRFLKTKAWADTECIVVGGGFRKSRVGEIAIARADLLLKNEGHKVHLVPIRFDPDEAGLIGCLHLAPSWIFEAFDSILAVDIGGTNIRCGVVETKWKKASDLSRAGVWRSELWRHADDEPTREGAVKKLVKMLKDLVSQTEKQGLKLAPFIGISCPGVIEEDGSIKKGAQNLPGNWESSRFNLPKSLIEAIPEIGGHDTTVLMHNDGVAQGLSEMPYMQDVSRWGVLTIGTGLGNARFTNRRKENKKEKKPEDGKDKDKKDRKAKD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVJ4</accession>
    <name>ATPA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP synthase subunit alpha</fullName>
            <ecNumber evidence="1">7.1.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP synthase F1 sector subunit alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">F-ATPase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpA</name>
        <name type="ordered locus">Nwi_0430</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Produces ATP from ADP in the presence of a proton gradient across the membrane. The alpha chain is a regulatory subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + 4 H(+)(in) + H2O = ADP + 5 H(+)(out) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a(1), b(2) and c(9-12). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. CF(1) is attached to CF(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313761.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVJ4"/>
    <dbReference type="SMR" id="Q3SVJ4"/>
    <dbReference type="STRING" id="323098.Nwi_0430"/>
    <dbReference type="PRIDE" id="Q3SVJ4"/>
    <dbReference type="EnsemblBacteria" id="ABA03697">
        <property type="protein sequence ID" value="ABA03697"/>
        <property type="gene ID" value="Nwi_0430"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0430"/>
    <dbReference type="eggNOG" id="COG0056">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010091_2_1_5"/>
    <dbReference type="OMA" id="LQAPGVM"/>
    <dbReference type="OrthoDB" id="837522at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045261">
        <property type="term" value="C:proton-transporting ATP synthase complex, catalytic core F(1)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18113">
        <property type="entry name" value="ATP-synt_F1_alpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01132">
        <property type="entry name" value="F1_ATPase_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.150.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01346">
        <property type="entry name" value="ATP_synth_alpha_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000793">
        <property type="entry name" value="ATP_synth_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038376">
        <property type="entry name" value="ATP_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033732">
        <property type="entry name" value="ATP_synth_F1_a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005294">
        <property type="entry name" value="ATP_synth_F1_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00306">
        <property type="entry name" value="ATP-synt_ab_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039088">
        <property type="entry name" value="F_ATPase_subunit_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="SSF50615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00962">
        <property type="entry name" value="atpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0139">CF(1)</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000238303" description="ATP synthase subunit alpha">
        <location>
            <begin position="1"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="169"/>
            <end position="176"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Required for activity" evidence="1">
        <location>
            <position position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01346"/>
        </source>
    </evidence>
    <sequence length="510" mass="55182" checksum="B7DE29FA330E14F6" modified="2005-10-11" version="1">MDIRAAEISAILKDQIKNFGKEAEVSEVGQVLSVGDGIARVYGLDNIQAGEMVEFENGTRGMALNLETDNVGIVIFGADREIKEGQTVKRTRAIVDAPVGKGLLGRVVDALGNPIDGKGPIQGAERKRVDVKAPGIIPRKSVHEPMATGLKAIDALIPIGRGQRELIIGDRQTGKTAIALDTILNQKPLNVAGAPESQKLYCVYVAVGQKRSTVAQFVKVLEEQGALEYSIVVAATASDPAPMQYLAPFTGCTMGEYFRDNAMHAVIIYDDLSKQAVAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKLNDSQGNGSLTALPVIETQANDVSAYIPTNVISITDGQIFLETDLFFQGIRPAVNVGLSVSRVGSSAQTKAMKKVAGKIKGELAQYREMAAFAQFGSDLDASTQRLLNRGSRLTELLKQPQFSPLKMEEQVVVIWAGTNGYLDPLPLGKVRAFEDGLLSLLRGQHADILNTIRDTRDLSDETAGKLKSIVEGYAKTFA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-02-21" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVN2</accession>
    <name>ACCA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha</fullName>
            <shortName evidence="1">ACCase subunit alpha</shortName>
            <shortName evidence="1">Acetyl-CoA carboxylase carboxyltransferase subunit alpha</shortName>
            <ecNumber evidence="1">2.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">accA</name>
        <name type="ordered locus">Nwi_0392</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the acetyl coenzyme A carboxylase (ACC) complex. First, biotin carboxylase catalyzes the carboxylation of biotin on its carrier protein (BCCP) and then the CO(2) group is transferred by the carboxyltransferase to acetyl-CoA to form malonyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + N(6)-carboxybiotinyl-L-lysyl-[protein] = malonyl-CoA + N(6)-biotinyl-L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:54728"/>
            <dbReference type="Rhea" id="RHEA-COMP:10505"/>
            <dbReference type="Rhea" id="RHEA-COMP:10506"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:83144"/>
            <dbReference type="ChEBI" id="CHEBI:83145"/>
            <dbReference type="EC" id="2.1.3.15"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Acetyl-CoA carboxylase is a heterohexamer composed of biotin carboxyl carrier protein (AccB), biotin carboxylase (AccC) and two subunits each of ACCase subunit alpha (AccA) and ACCase subunit beta (AccD).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AccA family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.15" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313723.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVN2"/>
    <dbReference type="SMR" id="Q3SVN2"/>
    <dbReference type="STRING" id="323098.Nwi_0392"/>
    <dbReference type="PRIDE" id="Q3SVN2"/>
    <dbReference type="EnsemblBacteria" id="ABA03659">
        <property type="protein sequence ID" value="ABA03659"/>
        <property type="gene ID" value="Nwi_0392"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0392"/>
    <dbReference type="eggNOG" id="COG0825">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015486_0_2_5"/>
    <dbReference type="OMA" id="TPWQRVQ"/>
    <dbReference type="OrthoDB" id="886663at2"/>
    <dbReference type="UniPathway" id="UPA00655">
        <property type="reaction ID" value="UER00711"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009317">
        <property type="term" value="C:acetyl-CoA carboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016743">
        <property type="term" value="F:carboxyl- or carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001295">
        <property type="term" value="P:malonyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00823">
        <property type="entry name" value="AcetylCoA_CT_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001095">
        <property type="entry name" value="Acetyl_CoA_COase_a_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42853">
        <property type="entry name" value="PTHR42853"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03255">
        <property type="entry name" value="ACCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01069">
        <property type="entry name" value="ACCCTRFRASEA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00513">
        <property type="entry name" value="accA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword id="KW-0276">Fatty acid metabolism</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000223791" description="Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha">
        <location>
            <begin position="1"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="2">
        <location>
            <begin position="41"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01137"/>
        </source>
    </evidence>
    <sequence length="320" mass="34474" checksum="DBE2CB2108CB3D13" modified="2005-10-11" version="1">MPDPMRSYLDFEKPVAELDSKIDELRALAASGSDISEEISSIEEKAAQALKDLYANLTPWQKTQVARHPQRPHFSDFVKGLITEFTPLAGDRKFGEDEALIGGFGRFRGESICVIGQEKGATTESRLKHNFGMARPEGYRKAVRLMDMADRFDIPVLSLVDSAGAYPGIGAEERGQAEAIARSTDACLSLGVANIAVIIGEGGSGGAIAIATANRVLMLEHAIYSVISPEAASSILWRDSSKAQEAATNMKITAQDLLRFGVIDAILKEPAGGAHRDPAAVIALTGDAIAGALNDLRNLDRDSLRQQRRQKFIDIGRKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVN3</accession>
    <name>Q3SVN3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_0391</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YkuD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313722.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0391"/>
    <dbReference type="EnsemblBacteria" id="ABA03658">
        <property type="protein sequence ID" value="ABA03658"/>
        <property type="gene ID" value="Nwi_0391"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0391"/>
    <dbReference type="eggNOG" id="COG3034">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032558_3_0_5"/>
    <dbReference type="OMA" id="LSFNTGY"/>
    <dbReference type="OrthoDB" id="1654458at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071972">
        <property type="term" value="F:peptidoglycan L,D-transpeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16913">
        <property type="entry name" value="YkuD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005490">
        <property type="entry name" value="LD_TPept_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038063">
        <property type="entry name" value="Transpep_catalytic_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141523">
        <property type="entry name" value="SSF141523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229287" evidence="5">
        <location>
            <begin position="24"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="336"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="402"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="402"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="438"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="453"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA03658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="492" mass="52706" checksum="CE2EAA7BBC6FA886" modified="2005-10-11" version="1">MINRSIARTIMTSAVLMGSALLAGCNTDDASLASNAKANRPVSPKLVAAMTAKDMELQSPILIRLFKQEAELEVWKQDRSGKFALLKSYPICRWSGDLGPKTREGDRQAPEGFYSITPAQMNPRSSYYLSFNTGYPNEFDKSLGRTGSQLMVHGDCSSRGCYAMTDEQIAEIYAVGRESFFGGQRAFQLQAYPFRMTAANLAKHRNNPNMPFWKMIKEGNDHFEVTRQEPKVAFCERKYVFDPAPPPGSTRPLEFNASARCPAYTVPDNIASAVREKQQRDNTEIARLISKGAPVARLNTGIDGGMNRVFAAKLPGGQTGLSDPGESDVKATAFSAAPGTIPPTVNPPRNSAPATAATSYEPEAEVRPSSALLAPSKPSNIFTNLAHKMGFGDADTTATTKAAVSATPGESVSARPKTATPARSVSSRPVGAKRAASKTTEKTAEKTPRSEHVATARPQSGSTNVSASPARIQSAAPAASSNSFDSRFSVNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVP2</accession>
    <name>Q3SVP2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0382</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313713.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0382"/>
    <dbReference type="EnsemblBacteria" id="ABA03649">
        <property type="protein sequence ID" value="ABA03649"/>
        <property type="gene ID" value="Nwi_0382"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0382"/>
    <dbReference type="eggNOG" id="COG5319">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112041_1_0_5"/>
    <dbReference type="OMA" id="ARRMHYG"/>
    <dbReference type="OrthoDB" id="1914830at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009562">
        <property type="entry name" value="DUF1178"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06676">
        <property type="entry name" value="DUF1178"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032131">
        <property type="entry name" value="UCP032131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="147" mass="16408" checksum="3E52E8BE21AAB040" modified="2005-10-11" version="1">MIRYDLRCDRGHTFESWFQSSSAYDSQVKRKLVTCAVCGSAKVEKAIMAPRIAGSKKRPSRAPADSSMSATVAEERELRAELRQLHDHLVKNADNVGARFPNEARKMHYGDIEHRPIYGEASPEEAKSLIEEGVEVGSLPVPPDDRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVU0</accession>
    <name>Q3SVU0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0334</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0334"/>
    <dbReference type="EnsemblBacteria" id="ABA03601">
        <property type="protein sequence ID" value="ABA03601"/>
        <property type="gene ID" value="Nwi_0334"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0334"/>
    <dbReference type="eggNOG" id="ENOG502ZRD4">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1202931_0_0_5"/>
    <dbReference type="OMA" id="DDNAQWR"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004866">
        <property type="term" value="F:endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021140">
        <property type="entry name" value="Inh/Omp19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016085">
        <property type="entry name" value="Protease_inh_b-brl_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02974">
        <property type="entry name" value="Inh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50882">
        <property type="entry name" value="SSF50882"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229345" evidence="2">
        <location>
            <begin position="40"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Inh" evidence="3">
        <location>
            <begin position="46"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Inh" evidence="3">
        <location>
            <begin position="152"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="246" mass="26712" checksum="1D2A0B0B36F0F556" modified="2005-10-11" version="1">MMAAPVFADNVDGGASVLRKRRAVLVAAALLMSCGPALSQDSPALKKNMIGQWELSTTERSRTCVVTLKGETAPQGLKLDLEPGCVASLPFTKDIAAWTVAGLDIVRLQTATGESVIDFTEVESGILEGRRENDGIYILQNLAEARSLARSMDQMIGDWAFVRSNGQPICGITLTNTEAPPDSFQAFLKPKCDPAIAGFKPEVWRLDRGEILLMSAGGETWHFQADDNAQWRLVPDSANPLILIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW00</accession>
    <name>Q3SW00_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0274</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313607.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0274"/>
    <dbReference type="EnsemblBacteria" id="ABA03541">
        <property type="protein sequence ID" value="ABA03541"/>
        <property type="gene ID" value="Nwi_0274"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0274"/>
    <dbReference type="eggNOG" id="COG3311">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_191265_0_0_5"/>
    <dbReference type="OMA" id="ANPWDDE"/>
    <dbReference type="OrthoDB" id="2076478at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="81" mass="9154" checksum="ABD0784DB6C55243" modified="2005-10-11" version="1">MEKAKATIIPLSPRGLSRAQAAAYIGVSPSLFDELVEDGRMPRPKRINSRTVWDRIRLDEAFEAILDDESRAANPWDDEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW14</accession>
    <name>Q3SW14_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0260</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313593.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0260"/>
    <dbReference type="EnsemblBacteria" id="ABA03527">
        <property type="protein sequence ID" value="ABA03527"/>
        <property type="gene ID" value="Nwi_0260"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0260"/>
    <dbReference type="eggNOG" id="ENOG502Z8GP">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047094_0_0_5"/>
    <dbReference type="OMA" id="VFHNPMA"/>
    <dbReference type="OrthoDB" id="1143092at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="477" mass="53098" checksum="9F2232A130D12BA8" modified="2005-10-11" version="1">MTQKETSPRENEQSAGLLLPLSERCFDLYALSLPRGPNFHPWSFRSAWSSGKDSSIGAVLQLSPAGPFATLILRRQVDHRFVLTHRKEMIGSEQEAFADLAANMQFSAPEPLRPGEKRRPLLLDTAGRTPGDYFKLLTGTRTHYPALMAIGELYLAMPRPDDNFAGDFQTNNFNSRLWELYLFAAFREQDITVTQPLPSPDFLLERDGHSCWVEAVTANAEEPYAQGFTTPAPPPDDRAERLIGAPAERFAKTLRSKLQREYDKAPHVTGQSFAFAIADFHAPGSMVWSREALPSYLYGIHAQVGEGPEGPFAFAESVAKLRGSHSIPAGLFRDPAMAHLSAVIFSNAATLGKFNRMGLLAGWRPQGLSMVREGLIFDRTPGALEPKTFRLDVLSEEYARLWPGGEAWCQELEVFHNPMATHPIDFDLIPGATHWFEENGELMCSAYWECSVLSSMTHLKIETPKDGITQEKSKQAG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-04-08" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW48</accession>
    <name>COXX1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protoheme IX farnesyltransferase 1</fullName>
            <ecNumber evidence="1">2.5.1.141</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Heme B farnesyltransferase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Heme O synthase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ctaB1</name>
        <name type="ordered locus">Nwi_0225</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2E,6E)-farnesyl diphosphate + H2O + heme b = diphosphate + Fe(II)-heme o</text>
            <dbReference type="Rhea" id="RHEA:28070"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="ChEBI" id="CHEBI:60530"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.141"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; heme O biosynthesis; heme O from protoheme: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Carbon 2 of the heme B porphyrin ring is defined according to the Fischer nomenclature.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UbiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.141" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313560.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SW48"/>
    <dbReference type="SMR" id="Q3SW48"/>
    <dbReference type="STRING" id="323098.Nwi_0225"/>
    <dbReference type="EnsemblBacteria" id="ABA03493">
        <property type="protein sequence ID" value="ABA03493"/>
        <property type="gene ID" value="Nwi_0225"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0225"/>
    <dbReference type="eggNOG" id="COG0109">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029631_0_2_5"/>
    <dbReference type="OMA" id="QFFWQFP"/>
    <dbReference type="OrthoDB" id="1875497at2"/>
    <dbReference type="UniPathway" id="UPA00834">
        <property type="reaction ID" value="UER00712"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008495">
        <property type="term" value="F:protoheme IX farnesyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048034">
        <property type="term" value="P:heme O biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13957">
        <property type="entry name" value="PT_UbiA_Cox10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00154">
        <property type="entry name" value="CyoE_CtaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006369">
        <property type="entry name" value="Protohaem_IX_farnesylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030470">
        <property type="entry name" value="UbiA_prenylTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43448">
        <property type="entry name" value="PTHR43448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01473">
        <property type="entry name" value="cyoE_ctaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00943">
        <property type="entry name" value="UBIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0350">Heme biosynthesis</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000327097" description="Protoheme IX farnesyltransferase 1">
        <location>
            <begin position="1"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00154"/>
        </source>
    </evidence>
    <sequence length="312" mass="33564" checksum="52D442003D86F105" modified="2005-10-11" version="1">MSVVDQKAVELPPRISEAGVADYFALLKPRVMSLVIFTALVGLMIAPGHIHPVLGFIAILCIAVGAGASGALNMALEGDIDVLMSRTANRPIPRGRITRGEAMGFGLTLSFFSVMTLGALVNWYAGGLLAFTIFFYVVIYTMGLKRRTAQNIVIGGAAGALPPVVAWAAATGSLSVEPLLLFLIIFFWTPPHFWALALFRSDDYARAGVPMLPVVAGPDATRLQILLYTIVLVAIAAAPWPLGYFDWVYGVTSLILGAGMLVLAIEVYRHRTGSQALRATRRLFAFSILYLFALFAVLLLDVVAKAVAPLIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWA2</accession>
    <name>Q3SWA2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">3'-5' exonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0171</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313508.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0171"/>
    <dbReference type="EnsemblBacteria" id="ABA03439">
        <property type="protein sequence ID" value="ABA03439"/>
        <property type="gene ID" value="Nwi_0171"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0171"/>
    <dbReference type="eggNOG" id="COG0349">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086957_0_0_5"/>
    <dbReference type="OMA" id="MGLNPHR"/>
    <dbReference type="OrthoDB" id="1985371at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00474">
        <property type="entry name" value="35EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3'-5' exonuclease" evidence="2">
        <location>
            <begin position="1"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="206" mass="22996" checksum="50D9A9A89BE16B0F" modified="2005-10-11" version="1">MTIRLHRGDLPHQDLARYTGSAAIDTETMGLKPHRDRLCVVQMSPGDGSADVIQIARGQRDAPNLIKLLKDPAIVKIFHFARFDLAALYNAFGVMPRPVYCTKIASRLIRTYTDRHGLKDLVRELLSIDLSKQQQSSDWGSPTLSDAQLAYAASDVLHLHALRDKLEATLAREDRSALAQACFEFLPSRAMLDLAGWETEDIFAHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWC9</accession>
    <name>Q3SWC9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Electron transport protein SCO1/SenC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0144</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SCO1/2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313481.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0144"/>
    <dbReference type="EnsemblBacteria" id="ABA03412">
        <property type="protein sequence ID" value="ABA03412"/>
        <property type="gene ID" value="Nwi_0144"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0144"/>
    <dbReference type="eggNOG" id="COG1999">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050131_3_1_5"/>
    <dbReference type="OMA" id="FELNGWM"/>
    <dbReference type="OrthoDB" id="1994201at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02968">
        <property type="entry name" value="SCO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003782">
        <property type="entry name" value="SCO1/SenC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12151">
        <property type="entry name" value="PTHR12151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02630">
        <property type="entry name" value="SCO1-SenC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0186">Copper</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="4">
        <location>
            <begin position="37"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="197" mass="21545" checksum="E52FDCF7E801A6B7" modified="2005-10-11" version="1">MSNRFVRPLILVAAFAGSLVVGLTVVMWLLGGLASKPTAASIGGPFQLTDQTGQTVTEKDMVGRPTIVFFGYTHCPDVCPTSLFEMSEVLRAMGPDADRVNAYFITVDPERDTQETMKSYLSSFDPRLKGLTGDPAAVEKALSGFRVYAKKIPLKDGGYSMDHTALIYLMDRDGRFVSSFDLKRSAEAAAADLRRYL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWF7</accession>
    <name>Y116_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0102 protein Nwi_0116</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">Nwi_0116</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0102 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWF7"/>
    <dbReference type="SMR" id="Q3SWF7"/>
    <dbReference type="STRING" id="323098.Nwi_0116"/>
    <dbReference type="EnsemblBacteria" id="ABA03384">
        <property type="protein sequence ID" value="ABA03384"/>
        <property type="gene ID" value="Nwi_0116"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0116"/>
    <dbReference type="eggNOG" id="COG0792">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115353_0_2_5"/>
    <dbReference type="OMA" id="DIWLARQ"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00048">
        <property type="entry name" value="UPF0102"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003509">
        <property type="entry name" value="UPF0102"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34039">
        <property type="entry name" value="PTHR34039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02021">
        <property type="entry name" value="UPF0102"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00252">
        <property type="entry name" value="TIGR00252"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000336213" description="UPF0102 protein Nwi_0116">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00048"/>
        </source>
    </evidence>
    <sequence length="118" mass="13580" checksum="91C6930269AE2120" modified="2005-10-11" version="1">MRVAAFHAGLSAERRAAAYLIAKGYRILARRFRTPYGEIDIVARRRQLLAFVEVKARRSLDDAAYAVTRKQQQRIINAAQAWLMAHPEHETFEFRFDAMLIAPRRLPRHLLGAFDAST</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWG1</accession>
    <name>Q3SWG1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0112</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313449.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0112"/>
    <dbReference type="EnsemblBacteria" id="ABA03380">
        <property type="protein sequence ID" value="ABA03380"/>
        <property type="gene ID" value="Nwi_0112"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0112"/>
    <dbReference type="eggNOG" id="COG5321">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114402_0_0_5"/>
    <dbReference type="OMA" id="DYRAHCD"/>
    <dbReference type="OrthoDB" id="1539191at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009394">
        <property type="entry name" value="MmcB-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06319">
        <property type="entry name" value="MmcB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031796">
        <property type="entry name" value="UPC031796"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="163" mass="18125" checksum="1938837A9F24E113" modified="2005-10-11" version="1">METNAAHIALMVPPDRRQSATALAIARGTGRLLRSLGFACLSELSLPSGRRADLVAVNARGEIWIVEIKSSADDLRADRKWQDYRAHCDRLFFAFTRDLRCDIFPGDTGLIVADAYGAYLHCEAPEHRLPSPTRKAMMLRFGMAAAQRLNRLADPQGHAESEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWH8</accession>
    <name>Q3SWH8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chromosome segregation ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0095"/>
    <dbReference type="EnsemblBacteria" id="ABA03363">
        <property type="protein sequence ID" value="ABA03363"/>
        <property type="gene ID" value="Nwi_0095"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0095"/>
    <dbReference type="eggNOG" id="COG1192">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037612_1_4_5"/>
    <dbReference type="OMA" id="VQCEYFA"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_31" evidence="1">
        <location>
            <begin position="26"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="289" mass="31592" checksum="082204AD3327E56E" modified="2005-10-11" version="1">MAWFMTVIDDIDPRDDQGRWRARHPRIISLANQKGGVGKTTTAINLGTALAAIGERVLIVDLDPQGNASTGLGIDRRDRNVSTYDVLIGEAPLRDAVVPTAVPRLHIAASTMDLSGLELELGAAKDRAFRLRDAITALNGNAAYAADYTYVLIDCPPSLNLLTVNAMAASDAILVPLQCEFFALEGLSQLLQTVEQVRSTLNPNLSIHGIVLTMFDSRNNLSNQVVADVRQFMGSKVYDTMIPRNVRISEAPSYGKPVLVYDLKCSGSEAYLKLATEVIQRERELRMAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWI4</accession>
    <name>Q3SWI4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyridoxal phosphate homeostasis protein</fullName>
            <shortName evidence="1">PLP homeostasis protein</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0089</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Pyridoxal 5'-phosphate (PLP)-binding protein, which is involved in PLP homeostasis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the pyridoxal phosphate-binding protein YggS/PROSC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313426.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0089"/>
    <dbReference type="EnsemblBacteria" id="ABA03357">
        <property type="protein sequence ID" value="ABA03357"/>
        <property type="gene ID" value="Nwi_0089"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0089"/>
    <dbReference type="eggNOG" id="COG0325">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059988_1_1_5"/>
    <dbReference type="OMA" id="PLEWHMI"/>
    <dbReference type="OrthoDB" id="1423012at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02087">
        <property type="entry name" value="PLP_homeostasis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001608">
        <property type="entry name" value="Ala_racemase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011078">
        <property type="entry name" value="PyrdxlP_homeostasis"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10146">
        <property type="entry name" value="PTHR10146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01168">
        <property type="entry name" value="Ala_racemase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004848">
        <property type="entry name" value="YBL036c_PLPDEIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="SSF51419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00044">
        <property type="entry name" value="TIGR00044"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ala_racemase_N" evidence="4">
        <location>
            <begin position="28"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1 2">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR004848-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="224" mass="24000" checksum="975011D9134CB8A4" modified="2005-10-11" version="1">MTMGETAPVNGLASVEAEIVRACRDARRERSTVTLVAVSKTFDADAITPVIAAGQRVFGENRVQEAQRKWPELIAAHPGLALHLIGPLQSNKAKEAVALFDAIHSVDRVSLCEALAKEIDRQNRRPSLFVQINTGEEPQKAGVSPSNADDFIARCRDTHGLTISGLMCIPPMDDAPAPHFALTAKIARRNGIKQLSMGMSADFAIAIALGATHIRVGSAIFGTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWK2</accession>
    <name>Q3SWK2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF2235 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0071</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0071"/>
    <dbReference type="EnsemblBacteria" id="ABA03339">
        <property type="protein sequence ID" value="ABA03339"/>
        <property type="gene ID" value="Nwi_0071"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0071"/>
    <dbReference type="eggNOG" id="COG3673">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_360110_0_0_5"/>
    <dbReference type="OMA" id="WIWPLEL"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018712">
        <property type="entry name" value="DUF2235"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09994">
        <property type="entry name" value="DUF2235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="458"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="488"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="519"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="588"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2235" evidence="2">
        <location>
            <begin position="34"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="777" mass="87598" checksum="27F5B07B16DA6828" modified="2005-10-11" version="1">MPMRTTPQRASFVLEQECRDQHRANIVPGAGMGRKIVLLSDGTGNSAAKVWRTNVWRTFEALDVSGSDQVAFYDDGVGTSSFKPLAMLGGAFGFGLKRNVIDIYKFACRNWRDDSDEIFGFGFSRGAFTIRVVMGLILNQGLIVAATEKELDRKARAAYRAYRRERYHTVWRVEHLFRSLRDLVRRDDYDKADNRVIQRIRFIGVWDTVAAYGLPIDEMTRGISRFIWPLQFPDHRLDLTRVVRACQALSIDEERTTFHPELWDECGARSASPRRDGERYLADEQVSQVWFPGVHSNVGGGYPDDSLAYIPLVWMLREAEACGLRFKSDQGTPPADPDTFKHAIVNCDKDGRLYDPRKGVGGYYRYGPRKIELLSNYIYAKRDVVSVARPKVHESALCRIASRVHAYAPVGLPAVYDVATYDGRIVTPDAFGLETSGEAATRAEAQEHVWNEVWKRRIVYFLTVGVSLWLALYPLLRKLPAKDEYLSPFRWISDIIRLVGGFLPGFAQTWIDGYARSPVQFSLLFAAVIVLIAWSGRLAAGISDTMGAIWRRAPSAPVTRPTNWIYRLRTSRIYTAFHDWLKRRGAPAFFALLFVYLGVTLTSHLLYNVQDIAGFTCRESPDTKRLARGEEASATFATSGLCNNTGIFVEGNGARYLVKVETTEPFRDGGIASPLGGFYSTDAPDWHQQLALVLGVPLRRELTRPWFRLVLRYGAVGGEEVFLDPDPEDGTIEAVIRPTRSGELLVFVNDAVLGVPGFYDTFYRNNEGAAKLIVKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWL3</accession>
    <name>Q3SWL3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313399.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0060"/>
    <dbReference type="EnsemblBacteria" id="ABA03328">
        <property type="protein sequence ID" value="ABA03328"/>
        <property type="gene ID" value="Nwi_0060"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0060"/>
    <dbReference type="eggNOG" id="COG2981">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081565_0_0_5"/>
    <dbReference type="OMA" id="LGREYFT"/>
    <dbReference type="OrthoDB" id="1914427at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="246" mass="26334" checksum="6A7C697DF28D0BAC" modified="2005-10-11" version="1">MIGAAVNALTQILSLPMRAILWRVIGLALVLIVVAAVGLQRLLSWLAGSGEVWAETMLGPSFHTPLNILAWILSIAAGLGIVFGAVFLMPAITSLVAGVFVDDVAEIVEHEHYPADRPGAALPIGLAMTESIKAALLTLLVYLIALPFVFVAGAGVIAFFIATAWLLGREYFELAAMRFRSPAEAKAMRRDNASTIFMAGLIIAAFVAIPVVNLATPLFGMAFMVHLHKRLSGPRPELIAPDRRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="127" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWP1</accession>
    <name>Q3SWP1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Enoyl-[acyl-carrier-protein] reductase [NADH]</fullName>
            <ecNumber evidence="7">1.3.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_0032</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>a 2,3-saturated acyl-[ACP] + NAD(+) = a (2E)-enoyl-[ACP] + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:10240"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9926"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78785"/>
            <dbReference type="EC" id="1.3.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.9" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313371.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0032"/>
    <dbReference type="EnsemblBacteria" id="ABA03300">
        <property type="protein sequence ID" value="ABA03300"/>
        <property type="gene ID" value="Nwi_0032"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0032"/>
    <dbReference type="eggNOG" id="COG0623">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_10_1_5"/>
    <dbReference type="OMA" id="WMGPAKA"/>
    <dbReference type="OrthoDB" id="762291at2"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004318">
        <property type="term" value="F:enoyl-[acyl-carrier-protein] reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016631">
        <property type="term" value="F:enoyl-[acyl-carrier-protein] reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05372">
        <property type="entry name" value="ENR_SDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014358">
        <property type="entry name" value="Enoyl-ACP_Rdtase_NADH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43159">
        <property type="entry name" value="PTHR43159"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000094">
        <property type="entry name" value="Enoyl-ACP_rdct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="7" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 7 9" id="KW-0520">NAD</keyword>
    <keyword evidence="4 7 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="20"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="65"/>
            <end position="66"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="192"/>
            <end position="196"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000094-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000094-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA03300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="277" mass="29357" checksum="C92262C97D04A611" modified="2005-10-11" version="1">MTHLMQGKRGLIMGVANDHSIAWGIAKTLAAHGAEMAFTYQGEALAKRVRPLAESVGSTMVLPCDVEDIASVDKVFADIGKAWGKMDFLVHAIAFSDKAELNGRYADTTRENFARTMLISCFAFTEAAKRAAELMPDGGSMLTLTYNGGDRAMPNYNVMGVAKAGLEASVRYLAVDFGAQNIRVNAISAGPIRTLAGAGIGDARFMFAFQQKHSPRGRGVSLDELGGAGLYLLSDLSGGVTGEIHFVDSGYNIIAMPQPDALRSEGMSNGAPNDTAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMV3</accession>
    <name>Q3SMV3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_3141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316296.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3141"/>
    <dbReference type="EnsemblBacteria" id="ABA06388">
        <property type="protein sequence ID" value="ABA06388"/>
        <property type="gene ID" value="Nwi_3141"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3141"/>
    <dbReference type="eggNOG" id="ENOG5033AZT">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_168239_1_0_5"/>
    <dbReference type="OMA" id="LGEFWND"/>
    <dbReference type="OrthoDB" id="1933964at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="104" mass="11654" checksum="BA0AE86E6C5E584E" modified="2005-10-11" version="1">MIRAILRFIGLLLLAGGFIFLIYDGARSIADQALRLTKLGEFWNDIHQASQHAVRTQIEALAPWMWDLVKLALNQPAFAVLGVAGILLMLLFRPRPPLIGYARD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMX5</accession>
    <name>Q3SMX5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_3119</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316274.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3119"/>
    <dbReference type="EnsemblBacteria" id="ABA06366">
        <property type="protein sequence ID" value="ABA06366"/>
        <property type="gene ID" value="Nwi_3119"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3119"/>
    <dbReference type="eggNOG" id="COG3943">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048266_0_0_5"/>
    <dbReference type="OMA" id="TRVECRF"/>
    <dbReference type="OrthoDB" id="1838027at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011204">
        <property type="entry name" value="Virulence_RhuM-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13310">
        <property type="entry name" value="Virulence_RhuM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015268">
        <property type="entry name" value="Virulence_RhuM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="342" mass="39058" checksum="6FB5EC423C8EA649" modified="2005-10-11" version="1">MSEGELILYSTEDGAATVGLRAVDGTVWLSQREIAELFDKDVRTVNEHIRNVFAEGECDPEATIRKFRIVQTEGARQVERGVDAYNLDVILSVGYRVRSARGTQFRRWATTVLREYLVKGFVMDDARLKQADQWDYFDEWLARIRDIRASEKRFYHKVRDLYTTAIDYDKTSEQAQAFFKKVQNKMLWAVTGKTAAELIESRSDPDAPNMGLTSWKGSVVRKGDVGTAKNYLRAEEVEELNRIVVMYLDYAEDQAKRRRPVTMAEWSDKLDAFLSFNERDVLTHAGRLRMDVAQRLAAERFEVFNASRRAAEALAADAEDIAQLEEMEKAAKKGRKKGGGDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN13</accession>
    <name>Q3SN13_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FecR protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_3080</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316243.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3080"/>
    <dbReference type="EnsemblBacteria" id="ABA06328">
        <property type="protein sequence ID" value="ABA06328"/>
        <property type="gene ID" value="Nwi_3080"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3080"/>
    <dbReference type="eggNOG" id="COG3712">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050192_0_1_5"/>
    <dbReference type="OMA" id="ARISAMH"/>
    <dbReference type="OrthoDB" id="1381035at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006860">
        <property type="entry name" value="FecR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032623">
        <property type="entry name" value="FecR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012373">
        <property type="entry name" value="Ferrdict_sens_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30273">
        <property type="entry name" value="PTHR30273"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16220">
        <property type="entry name" value="DUF4880"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04773">
        <property type="entry name" value="FecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018266">
        <property type="entry name" value="FecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4880" evidence="2">
        <location>
            <begin position="8"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="FecR" evidence="1">
        <location>
            <begin position="114"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="317" mass="34700" checksum="A6BECF73317C0AF9" modified="2005-10-11" version="1">MHPDPVTDQALDWFIRLQEERDSATLAEFEAWRRSDMRCGQAFARISAMHGMASLRKATETDARRLGLDVPASAVSVRERKAPRSWIPAVAAAVLLLLIGWQQLPGVMLRWQSDYMTATGQRDTITLPDGSTATLNTSTAIAVDFSGGRRRVTLLDGEAYFDVQHDPSRPFVVAGHFAQVEVKGTAFSVRTENDQDTVVLERGRVEVSSQAGHEFLAPGQMIVASASGLSAVEAADIDKSLAWRDGRIVFRDRPFRAALADLERYFDGRVVVIGSVASDRLVSGHYRIDDPDAAIRTLARSAGADVTRLPGGILILR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN31</accession>
    <name>Q3SN31_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_3062</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316226.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3062"/>
    <dbReference type="EnsemblBacteria" id="ABA06310">
        <property type="protein sequence ID" value="ABA06310"/>
        <property type="gene ID" value="Nwi_3062"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3062"/>
    <dbReference type="eggNOG" id="COG0697">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067094_0_0_5"/>
    <dbReference type="OMA" id="GIFGYHA"/>
    <dbReference type="OrthoDB" id="1515911at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="7"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="150"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="288" mass="30063" checksum="49DB6208326A0F30" modified="2005-10-11" version="1">MTSRTATLIGLAAILMWSLLAVMTVATGRIPAFQLAAMTFAIGGLAGCVSWLFRPKGFRALRQPAAVWLVGVGGLFGYHALYFIALRQAPPAEAGLLNYLWPLLIVLFSAMLPGERLAPHHLIGSTLGLFGTVLLFAGNTSIVFERAQMSGLAAAFMAAFVWAAYSVLSRRLASVPTDVVAGFCLATAALAAVAHLLVEQTVWPDNAAQWLAIVALGIGPVGLAFYVWDIGVKRGDIRVLGAAAYATPLLSTSFLILAGFAKPTAALAVAAALIAGGGLIAAKDMIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN85</accession>
    <name>Q3SN85_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Formate/nitrite transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_3006</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316178.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3006"/>
    <dbReference type="EnsemblBacteria" id="ABA06256">
        <property type="protein sequence ID" value="ABA06256"/>
        <property type="gene ID" value="Nwi_3006"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3006"/>
    <dbReference type="eggNOG" id="COG2116">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036896_1_2_5"/>
    <dbReference type="OMA" id="MIWFPIM"/>
    <dbReference type="OrthoDB" id="1834167at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000292">
        <property type="entry name" value="For/NO2_transpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30520">
        <property type="entry name" value="PTHR30520"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01226">
        <property type="entry name" value="Form_Nir_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="48"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="78"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="165"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="199"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA06256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="272" mass="29452" checksum="7B657D8307E438C0" modified="2005-10-11" version="1">MADYANASVVIETVPPSDMGAAIAADAAKKAKYGTIHLLIRGFLCTPFLAYGASVVFAMVAAGIPLAVAGLVFPAGYITLSFLGLEMATGSFATMPIGMYAGTITPLQLVRNWFWTLGGNLLGGIFFGWLLWFSLTKGGAIEPDKMLQVIARTAEHKVEYIHYGLIGWCAAIGKGVLCNWLVSLGSVMSKATRSTVGRVVLMWIPIGMFFALGFEHTVVNMWLFPTAILSGANVSIYEWWVWNQIPVTIGNILGAMVLNGTLWYFTHTLQKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNC7</accession>
    <name>Q3SNC7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2964</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316136.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2964"/>
    <dbReference type="EnsemblBacteria" id="ABA06214">
        <property type="protein sequence ID" value="ABA06214"/>
        <property type="gene ID" value="Nwi_2964"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2964"/>
    <dbReference type="eggNOG" id="COG0508">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4249">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019739_1_0_5"/>
    <dbReference type="OMA" id="MTMSFRR"/>
    <dbReference type="OrthoDB" id="460797at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="330"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="540" mass="58354" checksum="780F7098392F56B7" modified="2005-10-11" version="1">MADYYPLIARAIAGLDVNAPGEQRRALYERARTALIAQLRGVEPPLTESEITRERLALEEAVRKVESEAAQRAREASRSSGSRSGDAFRAATRAAMKSPDVAAQPPVRSRANATPQSRNLRADSTPTSAPSPSPPPSVSPTPSWRPPGPPIPLPDEPPPPARERLGLGQNQNHNHQNQNQGQPRRGPEPDRPPPRPPVPGFRDVTADADDLGRAAAQANRSARRTYANVRSPSPEFDRLEPSLEDRGAFEGAYPSDQSFSGGAAVDQPTAPLPRSWPHPDRERPERVLGGGFPLKSAIVAGLLLILAGAGIIWGSNVVSAVRGLFDSSNQTSEMTKPVAPGLKPKITDRVGQPSMSETIAPVAQRVVLYDEDPSDPKGKQYVGSVVWRTEEVKGAAGSKPEIAVRAEIDIPERKFKMTMSFRRNTDTSLPASHTAELTFVLPPDFDGGGVSNVPGVLMKSNEQARGTPLAGLAVKVTDGFFLVGLSNVESDRTRNLQLMKERSWLDIPLVYTNQRRAIIAIEKGAPGERAFNEAFTEWGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SND3</accession>
    <name>Q3SND3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">UTP--glucose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="3">2.7.7.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Alpha-D-glucosyl-1-phosphate uridylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">UDP-glucose pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Uridine diphosphoglucose pyrophosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_2958</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-glucose 1-phosphate + H(+) + UTP = diphosphate + UDP-alpha-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:19889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.7.7.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UDPGP type 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.9" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316130.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="SMR" id="Q3SND3"/>
    <dbReference type="STRING" id="323098.Nwi_2958"/>
    <dbReference type="EnsemblBacteria" id="ABA06208">
        <property type="protein sequence ID" value="ABA06208"/>
        <property type="gene ID" value="Nwi_2958"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2958"/>
    <dbReference type="eggNOG" id="COG1210">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_1_2_5"/>
    <dbReference type="OMA" id="MHYVRQG"/>
    <dbReference type="OrthoDB" id="1402673at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003983">
        <property type="term" value="F:UTP:glucose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006011">
        <property type="term" value="P:UDP-glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02541">
        <property type="entry name" value="UGPase_prokaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005771">
        <property type="entry name" value="GalU_uridylyltTrfase_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43197">
        <property type="entry name" value="PTHR43197"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transferase" evidence="8">
        <location>
            <begin position="10"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA06208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="291" mass="31898" checksum="833E726CFFD5C8E0" modified="2005-10-11" version="1">MKIRKAVFPVAGLGTRVLPATKAMPKEMLTIVDRPLIQYVFDEAREAGIEHFIFVTGRNKSAIEDHFDRQFELDETLKTRGKHAEINLLAQFQPEAGAVSFTRQQAPLGLGHAVWCARDIVGHEPFAVVLPDELVLNTPGCLKQMMQAAANLGPKSNIVAVEAVPDDMTHNYGICGVGKRSDRTFEIDGMVEKPPKGTAPSNLSITGRYILQPEIFEILANQERGAGGEIQLTDAMIGLSKSQAFHGLEFAGERHDCGSRAGFLRANLAFGMARPDLREGLRAEMKKYLGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNE5</accession>
    <name>Q3SNE5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Response regulator receiver</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2946</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2946"/>
    <dbReference type="EnsemblBacteria" id="ABA06196">
        <property type="protein sequence ID" value="ABA06196"/>
        <property type="gene ID" value="Nwi_2946"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2946"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_8_5"/>
    <dbReference type="OMA" id="LHSAPVC"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="19"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="3">
        <location>
            <begin position="34"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA06196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="135" mass="15179" checksum="9AE6FD4167E4FBDB" modified="2005-10-11" version="1">MDYIKDCLSSRAPMISMHKILLAEDDNDMRRFLVKALENAGFKVSSYDNGLSAYQRLREEPFEMLLTDIVMPEMDGIELARRASELDPDIKIMFITGFAAVALNSDSEAPKNAKVLSKPVHLRELVSEVNKMLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNI9</accession>
    <name>Q3SNI9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">TonB-dependent receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_2902</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Cell outer membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TonB-dependent receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316082.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2902"/>
    <dbReference type="EnsemblBacteria" id="ABA06152">
        <property type="protein sequence ID" value="ABA06152"/>
        <property type="gene ID" value="Nwi_2902"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2902"/>
    <dbReference type="eggNOG" id="COG4772">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008287_13_2_5"/>
    <dbReference type="OMA" id="DTRWTHR"/>
    <dbReference type="OrthoDB" id="514956at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039426">
        <property type="entry name" value="BtuB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012910">
        <property type="entry name" value="Plug_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037066">
        <property type="entry name" value="Plug_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000531">
        <property type="entry name" value="TonB-dep_rcpt_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036942">
        <property type="entry name" value="TonB_rcpt_b-brl_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30069">
        <property type="entry name" value="PTHR30069"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07715">
        <property type="entry name" value="Plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00593">
        <property type="entry name" value="TonB_dep_Rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0798">TonB box</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229056" evidence="8">
        <location>
            <begin position="25"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="domain" description="Plug" evidence="10">
        <location>
            <begin position="90"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="TonB_dep_Rec" evidence="9">
        <location>
            <begin position="322"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="38"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA06152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="791" mass="85945" checksum="50336BC9CC327F7D" modified="2005-10-11" version="1">MFRSCLLSSAGALALFAVCDGAAAQTAASTLPAITVDAPRQAQPSPRKKRAVVQRGAPRSLRPQPAVAVMPSPAPTESLLATLHRAPIGQPQTTIGRDQFDNRPSFSVADVLRDSPGISVKQGNGPRDFGISIRGSNARNGFGIRNLVIFDDGFPVTQPDGLSRSDLIDPHAYGAIDVIRGPSSALYGNYATGGALNFRTRRGAEINGFEYGSDAGSYGYFNNYLTVGQKTGNFEASLFASDTRSDGYTGNTWFNTQTVNFLGTLQATPDDRLTFKLINNTLDARLAIRSSLNQFDANPFQRGCATAATAAPGCGTVSLFNNGFNPAAGTTPTTAEQAGFGRTDRRTIVGGRWEHDFDNQTTWRNQFVFDDRNISQPTGATGAIGDYPSYNLMSDIVRRGQIFGMDATGYLGVFYNTLSWSGDTYRVMPGGNASRGWLVSNLTGMHTNYGARARGEIQINESWSAIAGIGWETTHLKGVNTAYSYAGPTGIPATTINSVDRQLQNTAPEFALQYRADKDWLFRARVATGYGTPQVGNLFVLPNGQNGNNTQLNPQRNLGYDLGFDWTPDPTLRISATGFYEFFRDELVTQAAPLGAPNASYTFNAPKSEHRGVELLAQWRFLPSWRVTAAYTYLDQIYTSYTENINNGATFSFDRAGNKIPGISPNELTARLSYDQLSGALQGVGAFVELQWKDTFYMDNANLLKAPGYELVNLNVHYDTPLQSEYLKSFHLYFEVRNVFDKTFVASANNIANTVTAAGLQNPASVLANTGGSIYAGQPRMFIAGMRLAFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNL8</accession>
    <name>Q3SNL8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2873</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2873"/>
    <dbReference type="EnsemblBacteria" id="ABA06123">
        <property type="protein sequence ID" value="ABA06123"/>
        <property type="gene ID" value="Nwi_2873"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2873"/>
    <dbReference type="eggNOG" id="COG0476">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1179229_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008641">
        <property type="term" value="F:ubiquitin-like modifier activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="SSF69572"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="235" mass="24881" checksum="8129D173CD41B88A" modified="2005-10-11" version="1">MPETVDTLARLKERAAELGCSRGLGVFLDSLERSWEGYVLEAPVPIGIILCARKPEHLVGSISNIELLPYLVDIKALKGRAALFAAGDTEPVVPAMQIDATNPALLRDVSGAPDFAPVSILGCGSVGSKMAMHLARSGVTIGAVSDNGMLRPHNMARHALARASFAGSRACHRACAARTIARGARWRSGRRSSQEQATQNVPAEGSALCCQHNSIARRPRSSLGDRIEAGQGPVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNM2</accession>
    <name>Q3SNM2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2869</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316051.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2869"/>
    <dbReference type="EnsemblBacteria" id="ABA06119">
        <property type="protein sequence ID" value="ABA06119"/>
        <property type="gene ID" value="Nwi_2869"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2869"/>
    <dbReference type="eggNOG" id="COG1426">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153788_1_1_5"/>
    <dbReference type="OMA" id="DRVHARS"/>
    <dbReference type="OrthoDB" id="2063007at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="113" mass="12344" checksum="C7C95C46C2822D2B" modified="2005-10-11" version="1">MTAKNSLVQAPPFEVDRALKKLGADLRTARLRRNLTVDATAAKIGTGRRAILDAEKGKVSTQVGVYAGLLWAYGLIDRLGDAADPRFDEEGQRLALIQDRVHARSPKGLDNDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNN4</accession>
    <name>Q3SNN4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2857</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316045.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2857"/>
    <dbReference type="EnsemblBacteria" id="ABA06107">
        <property type="protein sequence ID" value="ABA06107"/>
        <property type="gene ID" value="Nwi_2857"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2857"/>
    <dbReference type="eggNOG" id="ENOG5032WFP">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_180803_0_0_5"/>
    <dbReference type="OMA" id="FTRFEIM"/>
    <dbReference type="OrthoDB" id="1872400at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="91" mass="10047" checksum="E29D5C2BE6402116" modified="2005-10-11" version="1">MSSTFRKQPAKSDLKRPARRGMARFEVVGRAADRDLIRSLAKRLAEDGAEADRIRATISQSMSTKPVRKAGILDALRRSPLVGASLDLARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNN6</accession>
    <name>Q3SNN6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2855</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2855"/>
    <dbReference type="EnsemblBacteria" id="ABA06105">
        <property type="protein sequence ID" value="ABA06105"/>
        <property type="gene ID" value="Nwi_2855"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2855"/>
    <dbReference type="eggNOG" id="COG4453">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152494_2_0_5"/>
    <dbReference type="OMA" id="LADQECF"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014795">
        <property type="entry name" value="Vibrio_phage_ICP1_Orf50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35401">
        <property type="entry name" value="PTHR35401"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08681">
        <property type="entry name" value="DUF1778"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="SSF47598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="102" mass="11731" checksum="33670C55BB2D05A1" modified="2005-10-11" version="1">MTMAQSATKSAQRRVRDERLETRVTADQKKLIERAAALQGRTVTDFVLTSVQEAARRAIEDHQTLHLSLRDSQAFVQALMKPLPVNDRLRDTVRRYRQRTGV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNN8</accession>
    <name>NHAA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Na(+)/H(+) antiporter NhaA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Sodium/proton antiporter NhaA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nhaA</name>
        <name type="ordered locus">Nwi_2853</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 H(+)(out) + Na(+)(in) = 2 H(+)(in) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29251"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:29252"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NhaA Na(+)/H(+) (TC 2.A.33) antiporter family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316041.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SNN8"/>
    <dbReference type="SMR" id="Q3SNN8"/>
    <dbReference type="STRING" id="323098.Nwi_2853"/>
    <dbReference type="EnsemblBacteria" id="ABA06103">
        <property type="protein sequence ID" value="ABA06103"/>
        <property type="gene ID" value="Nwi_2853"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2853"/>
    <dbReference type="eggNOG" id="COG3004">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015803_1_2_5"/>
    <dbReference type="OMA" id="WHTPFGF"/>
    <dbReference type="OrthoDB" id="1805428at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015081">
        <property type="term" value="F:sodium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006885">
        <property type="term" value="P:regulation of pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01844">
        <property type="entry name" value="NhaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023171">
        <property type="entry name" value="Na/H_antiporter_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004670">
        <property type="entry name" value="NhaA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30341">
        <property type="entry name" value="PTHR30341"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06965">
        <property type="entry name" value="Na_H_antiport_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00773">
        <property type="entry name" value="NhaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0050">Antiport</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0915">Sodium</keyword>
    <keyword id="KW-0739">Sodium transport</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000334347" description="Na(+)/H(+) antiporter NhaA">
        <location>
            <begin position="1"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="320"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="349"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="390"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="424"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01844"/>
        </source>
    </evidence>
    <sequence length="455" mass="48280" checksum="06CCC0B60BF03C62" modified="2005-10-11" version="1">MDRQTSPRDLPRAQFLAERALTTLERFLHVEAVSGIVLLVAAAVALIWANSPFAQSYHDLWHLPLSIGLGTFVYAQSLHFWVNDALMTVFFLVVGMEIRREIHEGALSKLDQAILPVAAAAGGVIVPALIYLVLNTDMVRAHGWAVPTATDIAFAVGVLALLGRSIPGNLRVFLLALAIIDDVVAVLIIAFFYSGGLDPSGFAIAALGVVMVVGLQRIGVGSAYAYVLPGAVIWVGLLVTGAHPTLAGVVLGLMTPVMSIPMREQPIDVLSRISEELRSHDAVSTKDARRLAHPRRQLRLANREMLPPVVRVQTALHPWVAYGVMPLFALANAGVSVGGVNLFAGEAHLAMLGVGLALVVGKPLGVVGATWFMVRMGWCRLPPGVSWGGILLIGLLAGIGFTMSIFIAILAFADENLLAAAKLGVLLGSSVAALLGLGWGALYVRRLRARTGAHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNP9</accession>
    <name>Q3SNP9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2840</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2840"/>
    <dbReference type="EnsemblBacteria" id="ABA06092">
        <property type="protein sequence ID" value="ABA06092"/>
        <property type="gene ID" value="Nwi_2840"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2840"/>
    <dbReference type="HOGENOM" id="CLU_2410274_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="92" mass="9814" checksum="1AE78007D184A20B" modified="2005-10-11" version="1">MARRAAARSSAAILRLSSGCGPTPSATKRRAQKGWSPMKGQIAVGLAIDAKDEKAARWYERFGAMPLLDSSPLSLILPFKTIIDALDAAQSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNQ9</accession>
    <name>Q3SNQ9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2829</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316022.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2829"/>
    <dbReference type="EnsemblBacteria" id="ABA06082">
        <property type="protein sequence ID" value="ABA06082"/>
        <property type="gene ID" value="Nwi_2829"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2829"/>
    <dbReference type="eggNOG" id="ENOG502Z85X">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088242_0_0_5"/>
    <dbReference type="OMA" id="ITPGEWE"/>
    <dbReference type="OrthoDB" id="1416801at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045622">
        <property type="entry name" value="DUF6441"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20039">
        <property type="entry name" value="DUF6441"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="210" mass="22723" checksum="D7377CFFE5250C4C" modified="2005-10-11" version="1">MKLGVSIVGDIVRLMDEEVKAGEKAVTSAMRDAGTGLKTAWRAQITGAGLGARLARTIRSEQFPKGRTSLNAAALVWSKAPVIVGAHDTGPLIRSKNGFWLTIPTPAAGKSLRGGRITPGEWERRTGLRLRFVYRRTGPSLLVAEGRLNSRGRAVASRSKTGRGLATVPIFLLVPQVRLPKRLDLARDAERMRDAVPGQIVANWVDGRMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNY7</accession>
    <name>Q3SNY7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Multi-sensor diguanylate cyclase/phosphodiesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2751</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2751"/>
    <dbReference type="EnsemblBacteria" id="ABA06004">
        <property type="protein sequence ID" value="ABA06004"/>
        <property type="gene ID" value="Nwi_2751"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2751"/>
    <dbReference type="eggNOG" id="COG5001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_70_49_5"/>
    <dbReference type="OMA" id="QIYLWEP"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011623">
        <property type="entry name" value="7TMR_DISM_rcpt_extracell_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011622">
        <property type="entry name" value="7TMR_DISM_rcpt_extracell_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07695">
        <property type="entry name" value="7TMR-DISM_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07696">
        <property type="entry name" value="7TMR-DISMED2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229070" evidence="2">
        <location>
            <begin position="28"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="212"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="253"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="307"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="332"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="364"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="3">
        <location>
            <begin position="397"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="4">
        <location>
            <begin position="473"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="6">
        <location>
            <begin position="558"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="domain" description="EAL" evidence="5">
        <location>
            <begin position="700"/>
            <end position="955"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA06004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="960" mass="105737" checksum="34C1B9813377880E" modified="2005-10-11" version="1">MTLHLVRRLAWLTLALMIFVAASPARAVDAVSVRSDAPAIDLTAILDHQHSDSDRIQVSTAPGTDGIVRRVEVRAREGGQNWVVFALANNTDDQLDRLIVAPHYRLVSSGLLWPDLGQSRIATITPSTGDRPERQESATADVFQITLDPGAVITFVMELRTDRLPQLYLWEPDAYKDKVNSFTLYQGIVIGISGLLALVLTILFVVKGSIMFPAAAALAWAVLVYIGVDFGFWGKVLDMPAGAERIWRASGEAILAATLLVFLFAYLNLNRWHVRYSHITLGWLFFLGSLVALALFDPAVASGIARISLALIAVFGFGLIVYLAVHGFDRAVLLIPTWFLLVVWVAAAGMAIEGKVVNDIVGPALLGGLVLIVMLIGFTVMQHAFAGGGGASGVVSDIERRALALTGAGDLVWDWDVSADKVFTSLETESLLGLKRGTLEGPAAGWLEVLHPLDQDRFRAALDSVLEQRRGRLVQDFRLRASDGHFMWFALKARPVVGSDGEVSRVVGTLADVTETRKAEERMLHDSVHDNLTGLPNRKLFIDRLNAVATLAKTMPGLRPTLMVIDFDRFKQVNDSVGVAVGDSILLTLARRLTRILKAQDTLARLSGDQFGLILMSEQDPPRITAFAETIRKTIRAPIAFNDREIFLTASIGLALSDPQTQLSDQMIKDAELAMYHSKRIGGDRIDVYKPAMRARKNDRLSLEAELRRAIERQEITVLYQPIVRLQDRSIAGFEALARWDHPKLGRMAPDEFITIAEEAGLIVDLGTFVMDQTARQLAVWQRAMRSQPPIFASVNVSSRQLLRHDLIHDIRSVLSRSSIARGTLKLELTESLVMENPEHAAQMLQRIRELGVGLSLDDFGTGYSSLSYLQRFPLDTIKIDQSFVRTSTRGTRPVILKSIIALAHDLGMDVVAEGAETDSDAVELYQMGCEYAQGFAFGEPMDVDATMRLLAQERLETAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="137" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP28</accession>
    <name>Q3SP28_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP-dependent zinc metalloprotease FtsH</fullName>
            <ecNumber evidence="7">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ftsH</name>
        <name evidence="11" type="ordered locus">Nwi_2710</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell inner membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
            <orientation evidence="7">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">In the central section; belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315909.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2710"/>
    <dbReference type="MEROPS" id="M41.001"/>
    <dbReference type="EnsemblBacteria" id="ABA05963">
        <property type="protein sequence ID" value="ABA05963"/>
        <property type="gene ID" value="Nwi_2710"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2710"/>
    <dbReference type="eggNOG" id="COG0465">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_16_1_5"/>
    <dbReference type="OMA" id="YDKQGGG"/>
    <dbReference type="OrthoDB" id="190468at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
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        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011546">
        <property type="entry name" value="Pept_M41_FtsH_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06480">
        <property type="entry name" value="FtsH_ext"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="SSF140990"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01241">
        <property type="entry name" value="FtsH_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 7 11" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="AAA" evidence="10">
        <location>
            <begin position="188"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="596"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="623"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="419"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="196"/>
            <end position="203"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="418"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="422"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="496"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA05963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="640" mass="69886" checksum="D1DE3C972114A409" modified="2005-10-11" version="1">MNANMRNFALWVIIVLLLLALFTLFQNPGHHTTAQDISFSQLLTEVDQNNVRDVVIQGQEIRGNFTNGSAFQTYAPNDPGLVKKLYDAKVQITAKPPGESVPWFVSLLVSWLPFIALIGVWIFLSRQMQGGAGKAMGFGKSRAKMLTEANGRVTFEDVAGVDEAKQDLQEIVEFLRDPGKFQRLGGRIPRGVLLVGPPGTGKTLIARAVAGEANVPFFTISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRHRGAGLGGGNDEREQTLNQLLVEMDGFEANEGVILIAATNRPDVLDPALLRPGRFDRQVVVPNPDVVGREQILKVHVRKVPLAPDVNLKTIARGTPGFSGADLMNLVNEAALTAARRNKRMVTQSEFEEAKDKVMMGAERKSLVMTEEEKLLTAYHEGGHAIVGLNVAATDPIHKATIIPRGRALGMVMQLPERDKLSMSLEQMTSRLAIMMGGRVAEEMIFGPEKVTSGAASDIDQATRLARMMVTRWGLSKELGTVSYGENNDEVFLGMQVNRQQNVSEATAQKIDSEVRRLVETGYKDATRILTEKRADLEALAKGLLEFETLTGDEITDLLNGKKPNRESVLEPATPRASAVPPAGKARPRPEPDPGLEPQPQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP83</accession>
    <name>Q3SP83_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Multisubunit potassium/proton antiporter, PhaC subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_2655</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315858.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2655"/>
    <dbReference type="EnsemblBacteria" id="ABA05908">
        <property type="protein sequence ID" value="ABA05908"/>
        <property type="gene ID" value="Nwi_2655"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2655"/>
    <dbReference type="eggNOG" id="COG1006">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082058_3_0_5"/>
    <dbReference type="OMA" id="YRVYEEH"/>
    <dbReference type="OrthoDB" id="2078994at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039428">
        <property type="entry name" value="NUOK/Mnh_C1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00420">
        <property type="entry name" value="Oxidored_q2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="112" mass="11654" checksum="AAA61C29F36C0928" modified="2005-10-11" version="1">MEIVLAIGIGVLAGSGVWLLLRPRTFQVIIGLSLLSYAINLFIVAMGRLKIDAAPILGSHAAADITGLPDPLPQALVLTAIVISFATTALFLVVLIASRGLTGTDHVDGREP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP86</accession>
    <name>Q3SP86_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cytochrome c biogenesis factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2652</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315855.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2652"/>
    <dbReference type="EnsemblBacteria" id="ABA05905">
        <property type="protein sequence ID" value="ABA05905"/>
        <property type="gene ID" value="Nwi_2652"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2652"/>
    <dbReference type="eggNOG" id="COG4235">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1302761_0_0_5"/>
    <dbReference type="OMA" id="KPALVKW"/>
    <dbReference type="OrthoDB" id="1696728at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="216" mass="23520" checksum="E137CC8F39A41694" modified="2005-10-11" version="1">MRKAPVDQTQRSRGWLKLYLGSSFAGAAVGMAVAVWFTYQSPLTTHQVTVPLNSAATNPPVPLEGLFDLPKLLAAEHASQPWAVMGGALLQLNLPRLSIPLLERAIKRDPDNSVLHVALGEALALADGGVISDRAKAEFERVLKGDPNDLIARFYMGKWLLQNGKAKPALVKWVGLMRTVGNDQTWNDRLWTVMPRAAEEVGISQLTLQALCTSGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPD4</accession>
    <name>Q3SPD4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glutathione S-transferase</fullName>
            <ecNumber evidence="4">2.5.1.18</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2604</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315807.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2604"/>
    <dbReference type="EnsemblBacteria" id="ABA05857">
        <property type="protein sequence ID" value="ABA05857"/>
        <property type="gene ID" value="Nwi_2604"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2604"/>
    <dbReference type="eggNOG" id="COG0625">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011226_14_4_5"/>
    <dbReference type="OMA" id="LSWLMFI"/>
    <dbReference type="OrthoDB" id="1560019at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="SSF47616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="84"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="227" mass="25730" checksum="E9810B390EEF6195" modified="2005-10-11" version="1">MIKFYFNGAPNPNKVALFLEEAGLPFELVPIDTRKGDQFKPEFLKVNPNGKVPAIDDDGVFVFDSNAILLYLGEKTGKFMPENTPAHRAQMLSWMMFIASGLGPYSGQAVHFKDYAPKGLDYALNRYQFEAHRHYKVLDDHLAGNRYMVGDTYSIVDMAFWGWARMAPRVIGDSVAKYPNFKRLLDEVSARPAAAKAIALKDKFTFKTENDEEALGFLYRHMKTKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPM3</accession>
    <name>Q3SPM3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Aldehyde dehydrogenase</fullName>
            <ecNumber evidence="3">1.2.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2515</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.1.3" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315719.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2515"/>
    <dbReference type="EnsemblBacteria" id="ABA05768">
        <property type="protein sequence ID" value="ABA05768"/>
        <property type="gene ID" value="Nwi_2515"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2515"/>
    <dbReference type="eggNOG" id="COG1012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_1_2_5"/>
    <dbReference type="OMA" id="CVFTTDL"/>
    <dbReference type="OrthoDB" id="744602at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043878">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (non-phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044638">
        <property type="entry name" value="ALDH7A1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43521">
        <property type="entry name" value="PTHR43521"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="2">
        <location>
            <begin position="23"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="502" mass="53904" checksum="9447077F681EF8B9" modified="2005-10-11" version="1">MLASEVSSLLDHLGVSSDRRRGAHAVRSPLTGETIAHVQDASPQYAAEAIALAETAFRRWREAPPPRRGELVRLLGHELRAAQDALGRLVTIEAGKIVPEGRGEVQEMIDICDFAVGLSRQLYGLTIASERPHHRMMEQWHPLGPTGIITSFNFPVAVWSWNAALALVCGNPVIWKPSEKTPLTALAVQTLFERAAAKAGEVPEGLSTVLIGGRDVGETLVNDPRVPLVSATGSTAMGREVGTQLARRFGRSILELGGNNGSIVCPSANLDLALRAIAFSAIGTAGQRCTTLRRLFVHDSIHDAFMAKLKGIYASVRIGDPLTSDDVLVGPLIDATAFESMQRALEEARAHGAHVHGGERVDKRQFPDAYYVRPALVEMPAHTGPVLHETFAPILYVMKYTDLDAAIEQHNAVAHGLSSSIFSTDMREVERFLSTTGSDCGIVNANIGPSGAEIGGAFGGEKETGGGREAGSDAWKAYMRRVTSTVNYGDDLPLAQGVRFEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPN5</accession>
    <name>Q3SPN5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2503</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2503"/>
    <dbReference type="EnsemblBacteria" id="ABA05756">
        <property type="protein sequence ID" value="ABA05756"/>
        <property type="gene ID" value="Nwi_2503"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2503"/>
    <dbReference type="eggNOG" id="COG1565">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024840_3_0_5"/>
    <dbReference type="OMA" id="YYHPQRN"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003788">
        <property type="entry name" value="NDUFAF7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038375">
        <property type="entry name" value="NDUFAF7_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12049">
        <property type="entry name" value="PTHR12049"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02636">
        <property type="entry name" value="Methyltransf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="390" mass="42630" checksum="491748C4113B0FC0" modified="2005-10-11" version="1">MRRRARIRALQPRSIVTEPAPLLTYIKKLIKTSGPLPVWRYMQLCLTHPEHGYYIARDPLGREGDFVTSPEVSQMFGELLGLWAASVWRMMGSPDPLRLIELGPGRGTLMADALRALRVLPPMYESLSVHMVEINPVLVEKQMAALSDAPNIEWHTSLDQVPQGPAIILANEYFDVLPVHQMVRRDGGWHERVVDIDGSGQLVFGVSAEPTPRFDVLLPPLVRAAPVGAIFEWRPDAEMMSIATRVRDNGGAALIIDYGHVRSDAGDTFQAISRHSFADPLKYPGRVDVTAHVDFEALARAAEDVGARVHGPVPQGEFLRRLGIEARAVNLMAKATPELSDDIATALKRLTEGGRGGMGSMFKVIGVSDPSLSVLVGLSDQAHGGGIRTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPP9</accession>
    <name>Q3SPP9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2489</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315695.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05742">
        <property type="protein sequence ID" value="ABA05742"/>
        <property type="gene ID" value="Nwi_2489"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2489"/>
    <dbReference type="eggNOG" id="ENOG5030U43">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1114897_0_0_5"/>
    <dbReference type="OMA" id="NVHTVRP"/>
    <dbReference type="OrthoDB" id="1879921at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="259" mass="26216" checksum="F6FBBFC6FF41E516" modified="2005-10-11" version="1">MLPGFRSLFVAVTLTISMLIFGLGAAALLRATHEEFASLSPKQTPDLTFGTGETQQPTLAVLQVDKPAAEPAPPEASQHEAQSAEAASPDARQETGAPAANTPTTDAPPATPTADTPAAPAEAAAADEAPAASPPPSGPVPSAADTTPANAPAPEPATGPGDEPVKAGEAVKPPGATTTPAAKSDAVPTDGKTSEAEETTTSPVKDDFKTPLPGQRPAEAAKTGDPVPSASKRSSAKTPRAAKRHSSHHHRHKNRHSHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPQ1</accession>
    <name>Q3SPQ1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2487</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05740">
        <property type="protein sequence ID" value="ABA05740"/>
        <property type="gene ID" value="Nwi_2487"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2487"/>
    <dbReference type="HOGENOM" id="CLU_2509318_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="85" mass="9977" checksum="5D3E055BC908997F" modified="2005-10-11" version="1">MAPSNIAHRRADRRSKRKEHPMADFRPHSFSQGTPRLDHRLRGLDRRSRPPHPGYEVTGSYSHYYIEMVETIAAWLKGARIRSPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPQ2</accession>
    <name>Q3SPQ2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2486</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315692.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05739">
        <property type="protein sequence ID" value="ABA05739"/>
        <property type="gene ID" value="Nwi_2486"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2486"/>
    <dbReference type="HOGENOM" id="CLU_196549_0_0_5"/>
    <dbReference type="OMA" id="FNSYRPE"/>
    <dbReference type="OrthoDB" id="2052354at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="67" mass="7757" checksum="8DE2AAA491F465CB" modified="2005-10-11" version="1">MATSVSASASNRTEKQDHQRGRLHSLRARWREFIAIAFNSYRPELHYMRGPGPAWRAKHGEKPPIRS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPQ7</accession>
    <name>TAM_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Trans-aconitate 2-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.144</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tam</name>
        <name type="ordered locus">Nwi_2481</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the S-adenosylmethionine monomethyl esterification of trans-aconitate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-adenosyl-L-methionine + trans-aconitate = (E)-3-(methoxycarbonyl)pent-2-enedioate + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:14969"/>
            <dbReference type="ChEBI" id="CHEBI:15708"/>
            <dbReference type="ChEBI" id="CHEBI:57470"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.1.1.144"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. Tam family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.144" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315687.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SPQ7"/>
    <dbReference type="SMR" id="Q3SPQ7"/>
    <dbReference type="STRING" id="323098.Nwi_2481"/>
    <dbReference type="EnsemblBacteria" id="ABA05734">
        <property type="protein sequence ID" value="ABA05734"/>
        <property type="gene ID" value="Nwi_2481"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2481"/>
    <dbReference type="eggNOG" id="COG4106">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_5_2_5"/>
    <dbReference type="OMA" id="RFDARYY"/>
    <dbReference type="OrthoDB" id="1739932at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030798">
        <property type="term" value="F:trans-aconitate 2-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00560">
        <property type="entry name" value="Tran_acon_Me_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023506">
        <property type="entry name" value="Trans-aconitate_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023149">
        <property type="entry name" value="Trans_acon_MeTrfase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000056569" description="Trans-aconitate 2-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00560"/>
        </source>
    </evidence>
    <sequence length="256" mass="29107" checksum="13F637F3D7DCCCC8" modified="2005-10-11" version="1">MDDWSAEQYLKFEDERTRPSRDLLAQIPLSAPRKVVDIGCGPGNSTELLVARWPQAEVIGIDTSADMLRRARERLPGYTFIEANVAHWVPPADADLLFANAIFQWVPDHLRQLQRLLGALPSGGVLAMQMPDNLDEPSHVMMREVAHSGPWRERLAEAARAKDLLARPGAYYDALRPLCERIEIWHTVYNHALEDTAAIVEWVKGTGLRPFVDLLEPMERKEFLREYTARVAAAYQPQADGKVLLRFPRFFVVAIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPS4</accession>
    <name>Q3SPS4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycine oxidase</fullName>
            <ecNumber evidence="2">1.4.3.19</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2464</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.4.3.19" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315672.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2464"/>
    <dbReference type="EnsemblBacteria" id="ABA05717">
        <property type="protein sequence ID" value="ABA05717"/>
        <property type="gene ID" value="Nwi_2464"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2464"/>
    <dbReference type="eggNOG" id="COG0665">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007884_1_0_5"/>
    <dbReference type="OMA" id="CRRLEPM"/>
    <dbReference type="OrthoDB" id="912110at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043799">
        <property type="term" value="F:glycine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAO" evidence="1">
        <location>
            <begin position="14"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="338" mass="37617" checksum="D795099478B87CD4" modified="2005-10-11" version="1">MLHKTWDRRGTPPIAVIGAGIAGAWQALLFAKAGHEVTLHERDNDAMTQATSHWAGGMLAPWCESEVSEPLVTRLGLRSLALWREHAPDTPFNGSLVVAHPRDRSDFERFARMTSGHQRLQRDDVSKLEPSLDGRFREALFFPDEGHVEPRRVLPELHARIRAAGGRIEFGSAPETTHIDGLEIDCRGLAARDAFPDLRGVKGEEIIVETDEIVLSRPIRILHPRWPLYIIPRERNRFMIGATSIEREDDGVSVRSTLELLSAAYAVHPAFGEARIVEIGAGLRPAFPDNLPRIAIDKDHIAVNGLYRHGFLLAPALAELTLGYVARGEIDNEVMQCK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPZ6</accession>
    <name>HIS52_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Imidazole glycerol phosphate synthase subunit HisH 2</fullName>
            <ecNumber evidence="1">4.3.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">IGP synthase glutaminase subunit 2</fullName>
            <ecNumber evidence="1">3.5.1.2</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">IGP synthase subunit HisH 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">ImGP synthase subunit HisH 2</fullName>
            <shortName evidence="1">IGPS subunit HisH 2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisH2</name>
        <name type="ordered locus">Nwi_2391</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to HisF for the synthesis of IGP and AICAR.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H(+) + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="4.3.2.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-glutamine = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of HisH and HisF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="4.3.2.10" evidence="1"/>
    <dbReference type="EC" id="3.5.1.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315606.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SPZ6"/>
    <dbReference type="SMR" id="Q3SPZ6"/>
    <dbReference type="STRING" id="323098.Nwi_2391"/>
    <dbReference type="EnsemblBacteria" id="ABA05645">
        <property type="protein sequence ID" value="ABA05645"/>
        <property type="gene ID" value="Nwi_2391"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2391"/>
    <dbReference type="eggNOG" id="COG0118">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071837_2_0_5"/>
    <dbReference type="OMA" id="DKVPHMG"/>
    <dbReference type="OrthoDB" id="1726024at2"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000107">
        <property type="term" value="F:imidazoleglycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01748">
        <property type="entry name" value="GATase1_IGP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00278">
        <property type="entry name" value="HisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010139">
        <property type="entry name" value="Imidazole-glycPsynth_HisH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42701">
        <property type="entry name" value="PTHR42701"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000495">
        <property type="entry name" value="Amidotransf_hisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01855">
        <property type="entry name" value="IMP_synth_hisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000231738" description="Imidazole glycerol phosphate synthase subunit HisH 2">
        <location>
            <begin position="1"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1" evidence="1">
        <location>
            <begin position="3"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="192"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00278"/>
        </source>
    </evidence>
    <sequence length="212" mass="23414" checksum="BE54EF9693DD2F48" modified="2005-10-11" version="1">MARVAIIDYGINNVRSVRNAVEYCGHEAVVTHGNDGIADASHIILPGVGAFGDAMKKIRDRGLDEILARGVCEAGKPLLAVCLGMQLLAKTSEEHADDGEFFQGLGLIEADVRRLRPKDPDLKIPHMGWNNITKLREHPILVNMRETNLAFYFVHSFAMSCENEDDVVGRATYGQDVTAIVARDNIVGTQFHPEKSQDSGIELMSNFLQWNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ04</accession>
    <name>Q3SQ04_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glycosyl transferase, family 4</fullName>
            <ecNumber evidence="7">2.7.8.13</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2383</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.8.13" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2383"/>
    <dbReference type="EnsemblBacteria" id="ABA05637">
        <property type="protein sequence ID" value="ABA05637"/>
        <property type="gene ID" value="Nwi_2383"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2383"/>
    <dbReference type="eggNOG" id="COG0472">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023982_3_0_5"/>
    <dbReference type="OMA" id="WRLLGHF"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008963">
        <property type="term" value="F:phospho-N-acetylmuramoyl-pentapeptide-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051992">
        <property type="term" value="F:UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine:undecaprenyl-phosphate transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000715">
        <property type="entry name" value="Glycosyl_transferase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22926">
        <property type="entry name" value="PTHR22926"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00953">
        <property type="entry name" value="Glycos_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="182"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="234"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="281"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="311"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="333" mass="34872" checksum="30E47A3BAB7D3336" modified="2005-10-11" version="1">MTTIGIPLSLLAIITAAALSFALTWLLMPLLQRYALARPNARSSHKIPTPQGAGIAVIVATLATAVVFAFTIDAKISVYALAATVFIAVVGAADDIKSLPVLTRLLAQTIAVGAILSTASGHLRLFPVCPLWIEQGMLLLAGIWFVNLVNFMDGLDWMTVAEAVPIAGAIIALGYLGHIPPTTALVAGALGGALLGFAPFNRPLAKVFLGDVGSLPIGLLLGWCLLELAYQGHLAAALLLPFYYLADATITMLRRLVSGDKIWTAHRCHFYQRAADNGTPVIQIVGWVFAVNVLLAAMAIVSVAIPSPAVTALAMAGGATIVAVMLFRFSRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ24</accession>
    <name>Q3SQ24_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2363</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315579.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05617">
        <property type="protein sequence ID" value="ABA05617"/>
        <property type="gene ID" value="Nwi_2363"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2363"/>
    <dbReference type="eggNOG" id="ENOG50334N6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151314_0_0_5"/>
    <dbReference type="OMA" id="RCRGYYT"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229115" evidence="1">
        <location>
            <begin position="39"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="113" mass="11646" checksum="57FF765D6F934ADC" modified="2005-10-11" version="1">MRIKTKALPTESSFRLRIAVAVAAAALAVLSIPYPAHAQGVFGGMERGANQGAYEGNRAAGPVGGAIGGAIGAGVGGAVGGVRGVLGIPDRGPHRGQRCRGYWKRGHFYCYRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ81</accession>
    <name>Q3SQ81_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Biopolymer transport protein ExbD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_1268</name>
        <name evidence="13" type="ordered locus">Nwi_2306</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="13">Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the TonB-dependent energy-dependent transport of various receptor-bound substrates.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">The accessory proteins ExbB and ExbD seem to form a complex with TonB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the ExbD/TolR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314557.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1268"/>
    <dbReference type="EnsemblBacteria" id="ABA04529">
        <property type="protein sequence ID" value="ABA04529"/>
        <property type="gene ID" value="Nwi_1268"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05560">
        <property type="protein sequence ID" value="ABA05560"/>
        <property type="gene ID" value="Nwi_2306"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1268"/>
    <dbReference type="KEGG" id="nwi:Nwi_2306"/>
    <dbReference type="eggNOG" id="COG0848">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085305_1_3_5"/>
    <dbReference type="OMA" id="WMTVPAK"/>
    <dbReference type="OrthoDB" id="1940365at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003400">
        <property type="entry name" value="ExbD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014170">
        <property type="entry name" value="TonB_ExbD_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30558">
        <property type="entry name" value="PTHR30558"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02472">
        <property type="entry name" value="ExbD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02803">
        <property type="entry name" value="ExbD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA04529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA05560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="156" mass="16755" checksum="4D43475073B3F9F4" modified="2005-10-11" version="1">MGASIAEGGFDDDEEYGEAHEINVTPFIDVILVLLIIFMVAAPLSTVDLPVNLPTSSATPQKKPDKPTYVSIKADLAVAIGETLVKRVDLVRTLETTPNMTKESFIFLRADRAVPYGDLMDVLEILRTGGYAKIKLVALEGVPDARSVPAANTAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQA7</accession>
    <name>Q3SQA7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">OsmC-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315501.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2280"/>
    <dbReference type="ESTHER" id="nitwn-q3sqa7">
        <property type="family name" value="Est-OsmC"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05534">
        <property type="protein sequence ID" value="ABA05534"/>
        <property type="gene ID" value="Nwi_2280"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2280"/>
    <dbReference type="eggNOG" id="COG1073">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1765">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_681299_0_0_5"/>
    <dbReference type="OMA" id="AHCFTCS"/>
    <dbReference type="OrthoDB" id="721431at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003718">
        <property type="entry name" value="OsmC/Ohr_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036102">
        <property type="entry name" value="OsmC/Ohrsf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02566">
        <property type="entry name" value="OsmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82784">
        <property type="entry name" value="SSF82784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydrolase_4" evidence="1">
        <location>
            <begin position="48"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="406" mass="43780" checksum="36CF4161EE57A107" modified="2005-10-11" version="1">MPHERFQFSGSGGHKLAATLDLPDSEPVAYALFAHCFTCGKDVLAARRIATGLTGRGIAVLRFDFTGLGASEGEFANSTFSSNIADLVLAADHLRQTRKAPALLIGHSLGGAAVLAAAARIPEAEAVVTIAAPSDPAHVTRLFADHVTDIHEQGAVEVSLAGRPFPISRQFLDDIAEHNLTEQIANLRRALLVLHSPTDDIVGIDNATRIFVAAKHPKSFISLAGADHLLSEKRDTGYVAEVIASWAQRYLEPRPASAAENPESRDVVVRETGRGKFQQTVTVGPHRLKADEPIPVGGDDTGPGPYDLLLAALGACTSMTLRLYAERKALPLDRVTVRLRHSKVHAKDCAECETKTGTLDQIEREIAMEGTLDPDQRQKLIDIADKCPVHRTLTSEVRITTRTASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQF3</accession>
    <name>Q3SQF3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">7,8-dihydroneopterin aldolase</fullName>
            <ecNumber evidence="1">4.1.2.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2234</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of 7,8-dihydroneopterin to 6-hydroxymethyl-7,8-dihydropterin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7,8-dihydroneopterin = 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde</text>
            <dbReference type="Rhea" id="RHEA:10540"/>
            <dbReference type="ChEBI" id="CHEBI:17001"/>
            <dbReference type="ChEBI" id="CHEBI:17071"/>
            <dbReference type="ChEBI" id="CHEBI:44841"/>
            <dbReference type="EC" id="4.1.2.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate: step 3/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DHNA family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.25" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315456.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2234"/>
    <dbReference type="EnsemblBacteria" id="ABA05488">
        <property type="protein sequence ID" value="ABA05488"/>
        <property type="gene ID" value="Nwi_2234"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2234"/>
    <dbReference type="eggNOG" id="COG1539">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112632_1_1_5"/>
    <dbReference type="OMA" id="GHYKSVA"/>
    <dbReference type="OrthoDB" id="1858654at2"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00154"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004150">
        <property type="term" value="F:dihydroneopterin aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00534">
        <property type="entry name" value="DHNA_DHNTPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006156">
        <property type="entry name" value="Dihydroneopterin_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006157">
        <property type="entry name" value="FolB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42844">
        <property type="entry name" value="PTHR42844"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02152">
        <property type="entry name" value="FolB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00905">
        <property type="entry name" value="FolB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00525">
        <property type="entry name" value="folB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00526">
        <property type="entry name" value="folB_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="1 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FolB" evidence="2">
        <location>
            <begin position="5"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU362079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="121" mass="13035" checksum="812DC4AF82A4A6A8" modified="2005-10-11" version="1">MTDTIFIRGLVIHAQHGVMAHETEVGQRFVMDIELSTDLSESSRTDRLADTVSYAGVVATASAAFKDANYKLLERAAGAVADAVLAAYPRVRTITITVHKPHAPIPAIFDDVGISLTRGRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQH3</accession>
    <name>Q3SQH3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cytochrome c biogenesis protein, transmembrane region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2214</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315437.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2214"/>
    <dbReference type="EnsemblBacteria" id="ABA05468">
        <property type="protein sequence ID" value="ABA05468"/>
        <property type="gene ID" value="Nwi_2214"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2214"/>
    <dbReference type="eggNOG" id="COG0785">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053225_2_1_5"/>
    <dbReference type="OMA" id="TSLTPCM"/>
    <dbReference type="OrthoDB" id="1617952at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003834">
        <property type="entry name" value="Cyt_c_assmbl_TM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02683">
        <property type="entry name" value="DsbD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="210"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="DsbD" evidence="2">
        <location>
            <begin position="11"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="244" mass="25812" checksum="F741873C8E9BCCD2" modified="2005-10-11" version="1">MIHDVSVPAAMVAGLVSFLSPCVLPLVPPYLIYLTGATIEHVSHDETAKTSKRAVMLAALMFVFGFSTVFVALGASASFVGSFIRAWSAELSIVAGIVIIIMGLHFLGLTRIGFLMREGRLTAPKPVGLWGAYVMGLAFAFGWTPCIGPILAAILSVAAAEATVVKGAGLLAVYSAGLGIPFLLAALMIERFSSLFNRLKRHLTKIERGIGVLMVLTGVAFLTGTISNVSIWLLETFPALQNFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQH4</accession>
    <name>Q3SQH4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Amino acid/amide ABC transporter substrate-binding protein, HAAT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2213</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the leucine-binding protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315436.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2213"/>
    <dbReference type="EnsemblBacteria" id="ABA05467">
        <property type="protein sequence ID" value="ABA05467"/>
        <property type="gene ID" value="Nwi_2213"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2213"/>
    <dbReference type="eggNOG" id="COG0683">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027128_6_0_5"/>
    <dbReference type="OMA" id="YGVGFEQ"/>
    <dbReference type="OrthoDB" id="776281at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028081">
        <property type="entry name" value="Leu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000709">
        <property type="entry name" value="Leu_Ile_Val-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13458">
        <property type="entry name" value="Peripla_BP_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00337">
        <property type="entry name" value="LEUILEVALBP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229130" evidence="3">
        <location>
            <begin position="24"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="Peripla_BP_6" evidence="4">
        <location>
            <begin position="26"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="371" mass="40343" checksum="66F65FC0099363B9" modified="2005-10-11" version="1">MKSLKLICLALCASLALSATAFAQDITVAVAGPMTGSESAFGRQLHNGAEQFVADANDMGGVLNKKLKLFVGDDACDPKQARSIAERIASRGIPLVAGHFCSSSSIPASEAYADGNTLQITPATTNPLFTERKLWNVLRVCGRDDQQGIVAAEYIVKNFKDKNIAILNDKTTYGKGLADETKKALNKAGFQEKMFESYNKGDKDFNSIVSRLKRDNIDLVFIGGYYQEAGLILRQMRDQGLKTIMMAGDALNDKKFASITGPLAEGTLFTFGPDPRNKPTAKAVVEKFKARGIDPEGYTLYTYAAMQVWAQGAAKAGTTDPMKVMKAIKAGAWDTVLGKLEFDAKGDIRQIDYVIYKWDDKGNYAEIDPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQI1</accession>
    <name>Q3SQI1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Carbon monoxide dehydrogenase subunit G</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2206</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315429.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2206"/>
    <dbReference type="EnsemblBacteria" id="ABA05460">
        <property type="protein sequence ID" value="ABA05460"/>
        <property type="gene ID" value="Nwi_2206"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2206"/>
    <dbReference type="eggNOG" id="COG3427">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046420_1_0_5"/>
    <dbReference type="OMA" id="YEAIVTQ"/>
    <dbReference type="OrthoDB" id="1719670at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd05018">
        <property type="entry name" value="CoxG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010419">
        <property type="entry name" value="CO_DH_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38588">
        <property type="entry name" value="PTHR38588"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06240">
        <property type="entry name" value="COXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="148" mass="15700" checksum="06016D788133DF56" modified="2005-10-11" version="1">MAMTVNGEVELAASRDLVWAKLNDPEVLKSCIPGCEEFIKVDENQFQAVARLKIGPVSARFKARVTLSDIDPPNGYRIAAEGEGGVAGFAKGESTVVLLDHHGGTLLKYEAAAQIGGKLAQLGQRLISGTARKLAGEFFAKFAKAIEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQJ2</accession>
    <name>Q3SQJ2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Glycosyl transferase, family 2</fullName>
            <ecNumber evidence="9">2.4.1.83</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_2195</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GtrA family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.83" evidence="9"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315418.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2195"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05449">
        <property type="protein sequence ID" value="ABA05449"/>
        <property type="gene ID" value="Nwi_2195"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2195"/>
    <dbReference type="eggNOG" id="COG0463">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2246">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039727_0_0_5"/>
    <dbReference type="OMA" id="KLSWRVT"/>
    <dbReference type="OrthoDB" id="1064289at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004582">
        <property type="term" value="F:dolichyl-phosphate beta-D-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06442">
        <property type="entry name" value="DPM1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039528">
        <property type="entry name" value="DPM1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007267">
        <property type="entry name" value="GtrA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43398">
        <property type="entry name" value="PTHR43398"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04138">
        <property type="entry name" value="GtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="254"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="288"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="317"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="348"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="7">
        <location>
            <begin position="21"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="GtrA" evidence="8">
        <location>
            <begin position="257"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="376" mass="41198" checksum="8E0CD24AA9523177" modified="2005-10-11" version="1">MNQAIRPEPVEPSISMPRQLSVVVPTFNERDNVVALYQKLTAALKGIAWEVIYVDDNSPDGTSDVVRELAAVDPRVRCIRRVGRRGLSGACIEGILASSAPCAAVMDADLQHDETRLPAMHGLLESNAADLVVGSRYIEGGSADSFDKQRAGFSMLATSVAKKLLRIEIADPMSGFFMIRRDRFERLAPELSTQGFKILLDVIATARGALRVREIPYTFGSRLHGESKLDSMVALDFLGLVLAKLTHDAVSLRFLLFALVGSVGVVVHFVGLFVALEVLAWPFPHSQAFGAILAMTSNFLLNNFLTYRDQRLKGLGIVRGLLIFYLVCSVGLLANVGVAFSVYDQKPIWWLAGAAGALMGVVWNYAVSSLFVWRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQJ4</accession>
    <name>Q3SQJ4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2193</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2193"/>
    <dbReference type="EnsemblBacteria" id="ABA05447">
        <property type="protein sequence ID" value="ABA05447"/>
        <property type="gene ID" value="Nwi_2193"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2193"/>
    <dbReference type="HOGENOM" id="CLU_2684167_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="74" mass="8955" checksum="ABF28DB99ABFB2A8" modified="2005-10-11" version="1">MSYAQVEFLLADIAVKLELKFPYPIDKRIKAVRRIAEREGYEAYRFELEKACEDLLKYNDLRKSSAWFHFIDHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQK3</accession>
    <name>Q3SQK3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Aminopeptidase T, Metallo peptidase, MEROPS family M29</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_2184</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase M29 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315407.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2184"/>
    <dbReference type="MEROPS" id="M29.001"/>
    <dbReference type="EnsemblBacteria" id="ABA05438">
        <property type="protein sequence ID" value="ABA05438"/>
        <property type="gene ID" value="Nwi_2184"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2184"/>
    <dbReference type="eggNOG" id="COG2309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054346_1_0_5"/>
    <dbReference type="OMA" id="CNANIPT"/>
    <dbReference type="OrthoDB" id="689314at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1830.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035097">
        <property type="entry name" value="M29_N-terminal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000787">
        <property type="entry name" value="Peptidase_M29"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02073">
        <property type="entry name" value="Peptidase_M29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00919">
        <property type="entry name" value="THERMOPTASE"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA05438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="423" mass="45719" checksum="DF984B3B1F056D68" modified="2005-10-11" version="1">MTQHQRNIITPIDPVKLDRLAEVAVKVGLRLAPGQDLLITAPAVALPLVRRIAEHAYRAGAGLVTPFFSDEDITLSRYRFGPDQSFDRAAGWLYEGMAKAFAANTARLAIVGDNPMLLSGEDAAKVARANKANSVAYQPALEKIVGFDVNWNIVAYPGEAWARLMFPDDSPDGAVAKLAEAIFSASRVDDDDPVANWTAHNAALRQRTDWLNSQRFHALHYAGPDTDLTVGLADGHEWVGGASMARNGIICNPNIPTEEVFTTPHALRVEGRVRSTKPLSYQGTLIEGIAVRFEQGRIVEAHASRGADVLNKVLDTDEGARRLGEVALVPHSSPISKSGLLFYNTLFDENAASHIALGQCYSKCFVGGEKLSPEQIAAQGGNKSFIHIDWMIGSAETDIDGISGDGSRVPVFRKGEWAQAARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQM8</accession>
    <name>Q3SQM8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Methylmalonate-semialdehyde dehydrogenase (Acylating)</fullName>
            <ecNumber evidence="4">1.2.1.27</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2159</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.1.27" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2159"/>
    <dbReference type="EnsemblBacteria" id="ABA05413">
        <property type="protein sequence ID" value="ABA05413"/>
        <property type="gene ID" value="Nwi_2159"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2159"/>
    <dbReference type="eggNOG" id="COG1012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_1_10_5"/>
    <dbReference type="OMA" id="NWTLRQP"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004491">
        <property type="term" value="F:methylmalonate-semialdehyde dehydrogenase (acylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07085">
        <property type="entry name" value="ALDH_F6_MMSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010061">
        <property type="entry name" value="MeMal-semiAld_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43866">
        <property type="entry name" value="PTHR43866"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01722">
        <property type="entry name" value="MMSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="3">
        <location>
            <begin position="29"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="510" mass="55144" checksum="EC92BA545E7726DA" modified="2005-10-11" version="1">MSQRDYASEPEVNMRIIGHFIGGRNVEGASGRFSDVYQPMTGEVQARVALASKAELREAVENARAAQPAWAATNPQRRVRVLMKFLELVARDNDAIAELLAREHGKTIPDAHGDIFRGVEVVEYAIGIPELMKGDFTENVGPGIDSYSMRQPLGVVAGITPFNFPAMIPMWKFAPAIACGNAFILKPSERDPGVPMRLAELMLEAGLPPGILNVVNGDKEVVDAILDDPDIAAVGFVGSSSVAHYIYSRAAATGKRCQCFGGAKNHMIIMPDADMDQSVEALVGAGYGAAGERCMAVSVAVPVGEATADRLMEKLIPRVGSLKIGPSTDASADFGPLVTRAALERVRNYVEIGVKEGATLAVDGRGFKMQGYENGFYMGASLFDNVTRDMRIYKEEIFGPVLSVVRAKDFEEAVALPSEHDYGNGVAIFTRDGDAAREFASRVQVGMVGINVPIPVPVAYYTFGGWKRSAFGDLNQYGPDSVRFYTRTKTVTSRWPSDVKEGAQFAFHSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQN2</accession>
    <name>Q3SQN2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MaoC-like dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2155</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315378.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2155"/>
    <dbReference type="EnsemblBacteria" id="ABA05409">
        <property type="protein sequence ID" value="ABA05409"/>
        <property type="gene ID" value="Nwi_2155"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2155"/>
    <dbReference type="eggNOG" id="COG2030">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108911_1_0_5"/>
    <dbReference type="OMA" id="TVDQTMI"/>
    <dbReference type="OrthoDB" id="1380653at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd03450">
        <property type="entry name" value="NodN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002539">
        <property type="entry name" value="MaoC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039375">
        <property type="entry name" value="NodN-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01575">
        <property type="entry name" value="MaoC_dehydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MaoC-like" evidence="1">
        <location>
            <begin position="18"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="158" mass="17713" checksum="294BD7F48891E1BF" modified="2005-10-11" version="1">MNEVWKQPSVSLEAYRQMVGQEIGVSSWHLIDQKRIDLYADVIDDHQFIHVDPERARKTAFGATVAHGFLTMSLLSVMSHQVIPPVTGVTMRVNYGFDRLRFISPVKSGSRVRGRFTLVEATLRKPGELLSRTAVSVEIEGGTRPALAADWLALLYFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQP0</accession>
    <name>Q3SQP0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2147</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315370.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2147"/>
    <dbReference type="EnsemblBacteria" id="ABA05401">
        <property type="protein sequence ID" value="ABA05401"/>
        <property type="gene ID" value="Nwi_2147"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2147"/>
    <dbReference type="eggNOG" id="ENOG5033HZJ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2551642_0_0_5"/>
    <dbReference type="OMA" id="MHREVVE"/>
    <dbReference type="OrthoDB" id="1968804at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="82" mass="9160" checksum="3E28574A150C5B1F" modified="2005-10-11" version="1">MIYVLAALLPPLGLLFNGQPFSAILNVVVIVVCVIFGLIFPVLFLVPSAHAVIAVYMKREDRRHREVVDAIREHGPPPNYPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQQ4</accession>
    <name>Q3SQQ4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2132</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2132"/>
    <dbReference type="EnsemblBacteria" id="ABA05387">
        <property type="protein sequence ID" value="ABA05387"/>
        <property type="gene ID" value="Nwi_2132"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2132"/>
    <dbReference type="eggNOG" id="ENOG5032UMR">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108488_1_0_5"/>
    <dbReference type="OMA" id="FHATHGI"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="169" mass="17501" checksum="B7905867955FB5C6" modified="2005-10-11" version="1">MSRAPWWWWKARPSDPCLTEATIMILLSVLASIAAIGVICCLLFYLAVYALPLFAGVTAGVWAYGTGAGWLGGIAVGAVAGLATLLAGHLLLAFVRPLWLRLVIAAAFVAPAAIAGYHATHGIVKHTMPSETWQVAFSVAGAVVVAITALARLATLAPPGRTGQSVARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQX6</accession>
    <name>Q3SQX6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2057</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2057"/>
    <dbReference type="EnsemblBacteria" id="ABA05315">
        <property type="protein sequence ID" value="ABA05315"/>
        <property type="gene ID" value="Nwi_2057"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2057"/>
    <dbReference type="HOGENOM" id="CLU_2410273_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="92" mass="10415" checksum="2379A14162F7C1E2" modified="2005-10-11" version="1">MHRMRLLGRRKNHEMDQQQSDAADGYRFFVDDAARRLRIVSRLLTELSVRPSFLAIAAVPTPCSLSAINRSSSVGAHLRDDFCITAQRRTGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR01</accession>
    <name>Q3SR01_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Phosphate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2031</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315274.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2031"/>
    <dbReference type="EnsemblBacteria" id="ABA05290">
        <property type="protein sequence ID" value="ABA05290"/>
        <property type="gene ID" value="Nwi_2031"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2031"/>
    <dbReference type="eggNOG" id="COG0306">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015355_1_1_5"/>
    <dbReference type="OMA" id="RANPHKA"/>
    <dbReference type="OrthoDB" id="1329125at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001204">
        <property type="entry name" value="Phos_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101">
        <property type="entry name" value="PTHR11101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01384">
        <property type="entry name" value="PHO4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="220"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="263"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="309"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="334" mass="34866" checksum="89668A5F252822E3" modified="2005-10-11" version="1">MDAMLALPVLVGLIAVALLFDFLNGLHDAANSIATIVSTRVLRPQYAVVWAAFFNFIAFLVFGLHVANTIGTGIIDPNVIDAQVIFAALVGAIVWNLVTWGLGIPSSSSHALIGGLVGGGVAKAGLSAAVWGGLSKALLAIVLSPVVGFLLALVLVAIVSWLSVRSTPFAVDRAFRILQFFSASLYSLGHGGNDAQKTMGIIAVLLFSQGYLGSEFSVPFWVVLSCQAAMALGTLMGGWRIVRTMGLRITKLTPMQGFCAETGGAATLFMATFLGVPVSTTHTITGSIVGVGAARRLSAVRWNVASSIVYAWVITIPASAIVAALAYWAVAFVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR04</accession>
    <name>Q3SR04_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">SugE protein, small multidrug resistance protein, SMR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_2028</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the drug/metabolite transporter (DMT) superfamily. Small multidrug resistance (SMR) (TC 2.A.7.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05287">
        <property type="protein sequence ID" value="ABA05287"/>
        <property type="gene ID" value="Nwi_2028"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2028"/>
    <dbReference type="eggNOG" id="COG2076">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2826762_0_0_5"/>
    <dbReference type="OMA" id="MAWAILF"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042221">
        <property type="term" value="P:response to chemical"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000390">
        <property type="entry name" value="Small_drug/metabolite_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045324">
        <property type="entry name" value="Small_multidrug_res"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30561">
        <property type="entry name" value="PTHR30561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00893">
        <property type="entry name" value="Multi_Drug_Res"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="66" mass="7388" checksum="D6DB6BCFC055448B" modified="2005-10-11" version="1">MSWEDRMAWLLLFAAGVMEIGWAIGLKYTDGFTRLVPSMLTLAAMAISLGLLGLEHDEFRFGCILH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR20</accession>
    <name>Q3SR20_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cyclopropane-fatty-acyl-phospholipid synthase</fullName>
            <ecNumber evidence="2">2.1.1.79</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2012</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CFA/CMAS family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.79" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2012"/>
    <dbReference type="EnsemblBacteria" id="ABA05271">
        <property type="protein sequence ID" value="ABA05271"/>
        <property type="gene ID" value="Nwi_2012"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2012"/>
    <dbReference type="eggNOG" id="COG2230">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026434_6_1_5"/>
    <dbReference type="OMA" id="HRVEGIP"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008825">
        <property type="term" value="F:cyclopropane-fatty-acyl-phospholipid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003333">
        <property type="entry name" value="Mycolic_cyclopropane_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003085">
        <property type="entry name" value="CMAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="414" mass="47354" checksum="811183C8E277C289" modified="2005-10-11" version="1">MDRVLRFFLNQFVRRGSITFVTASGLTFTCGDGTGEPVRVRLTTSEVARQLLLDPEMSFGETYTDGTLLIERGSIADLLTVILDQPDVMPRWGKLQWWLRYLVRNIQQFNPRGRSRDNVARHYDLDGRLYSLFLDADQQYSCAYFETPDTTLDDAQLAKKRHIAAKLLLERDHSVLDIGSGWGGLGLYLAEMTGANLTGITLSTEQFQVSNARAVEKKLTRSAKFLLQDYRDIPGPFDRIVSVGMFEHVGVDHYDTFFKRCAGLLEPDGVMLLHSIGRSEGPGITNPWIAKYIFPGGYIPALSEVLPAIEGAGLLVCDIEVLRLHYAETLKAWRERFMARREEAVRLYDERFARMWEFYLAASEMSFRKQNLMNFQIQITKRQGIVPVTRDYIQRGEAKLRGLEQKHRPRLQIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR96</accession>
    <name>Q3SR96_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CRISPR-associated endoribonuclease Cas2</fullName>
            <ecNumber evidence="9">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cas2</name>
        <name evidence="11" type="ordered locus">Nwi_1935</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Functions as a ssRNA-specific endoribonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer, forms a heterotetramer with a Cas1 homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the CRISPR-associated endoribonuclease Cas2 protein family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="9"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315188.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1935"/>
    <dbReference type="EnsemblBacteria" id="ABA05195">
        <property type="protein sequence ID" value="ABA05195"/>
        <property type="gene ID" value="Nwi_1935"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1935"/>
    <dbReference type="eggNOG" id="COG1343">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161124_3_1_5"/>
    <dbReference type="OMA" id="SVFECEV"/>
    <dbReference type="OrthoDB" id="1951170at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09725">
        <property type="entry name" value="Cas2_I_II_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01471">
        <property type="entry name" value="Cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021127">
        <property type="entry name" value="CRISPR_associated_Cas2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019199">
        <property type="entry name" value="Virulence_VapD/CRISPR_Cas2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34405">
        <property type="entry name" value="PTHR34405"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09827">
        <property type="entry name" value="CRISPR_Cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032582">
        <property type="entry name" value="Cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01573">
        <property type="entry name" value="cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 10" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="5 9 10" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6 9 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 9 10" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR032582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA05195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="96" mass="10911" checksum="DFDAB455C91AB760" modified="2005-10-11" version="1">MLVLVTYDVATREEKGAARLRAVAKACRDYGQRVQYSVFEIEVDPAQWTRLKARLEAIIKPEFDSLRYYYLGANWQRKVEHVGAKPAADLGGPLIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRB5</accession>
    <name>Q3SRB5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1916</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315172.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1916"/>
    <dbReference type="EnsemblBacteria" id="ABA05176">
        <property type="protein sequence ID" value="ABA05176"/>
        <property type="gene ID" value="Nwi_1916"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1916"/>
    <dbReference type="eggNOG" id="ENOG5030WH8">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1977329_0_0_5"/>
    <dbReference type="OMA" id="YWFEVLL"/>
    <dbReference type="OrthoDB" id="1800376at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="123" mass="13582" checksum="8B24C75C247FC41D" modified="2005-10-11" version="1">MTIPRNVLRFEVLLYLSLLIDTLSAAFIARPSARVDRAAAASADTFVVMVFIFFLLLVWLAARQRKNWARWALVVALALSVLSLLGSLNSGLTPAFVIDFFSTGLAGAGLYFSFSDDAEDWFE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRI7</accession>
    <name>SYE_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamate--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glutamyl-tRNA synthetase</fullName>
            <shortName evidence="1">GluRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gltX</name>
        <name type="ordered locus">Nwi_1844</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)</text>
            <dbReference type="Rhea" id="RHEA:23540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.17"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="ABA05104" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05104.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_041344971.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRI7"/>
    <dbReference type="SMR" id="Q3SRI7"/>
    <dbReference type="STRING" id="323098.Nwi_1844"/>
    <dbReference type="PRIDE" id="Q3SRI7"/>
    <dbReference type="EnsemblBacteria" id="ABA05104">
        <property type="protein sequence ID" value="ABA05104"/>
        <property type="gene ID" value="Nwi_1844"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1844"/>
    <dbReference type="eggNOG" id="COG0008">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015768_6_0_5"/>
    <dbReference type="OrthoDB" id="1409413at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00808">
        <property type="entry name" value="GluRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00022">
        <property type="entry name" value="Glu_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045462">
        <property type="entry name" value="aa-tRNA-synth_I_cd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020751">
        <property type="entry name" value="aa-tRNA-synth_I_codon-bd_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008925">
        <property type="entry name" value="aa_tRNA-synth_I_cd-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004527">
        <property type="entry name" value="Glu-tRNA-ligase_bac/mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033910">
        <property type="entry name" value="GluRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19269">
        <property type="entry name" value="Anticodon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48163">
        <property type="entry name" value="SSF48163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00464">
        <property type="entry name" value="gltX_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000237376" description="Glutamate--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="1">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="1">
        <location>
            <begin position="240"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="474" mass="52622" checksum="51CF335EE1C326BA" modified="2006-05-30" version="2">MTTPVVTRFAPSPTGFLHIGGGRTALFNWLYARKHGGRMLLRIEDTDRARSTPEAIDAILDGLKWLGIEWDDDVVYQFSRVARHREIAEQLLAEGKAYRCYATTEELSEMREKARAEGRAKLYDGRWRDRDASEAPQGVKPTIRLKAPLTGETVIEDQVQGRIVWQNENLDDLVLLRGDGTPTYMLAVVVDDHDMGVTHIIRGDDHLINAARQKQIYDTLGWALPTMAHIPLIHGPDGSKLSKRHGALGVDAYRAMGYLPAALRNYLVRLGWSHGDQEIFSTQEMIDAFDLPAIGRSAARFDFAKLENLNGHYIRHCDDAALMTLFENALDFVPGGADLKPKLNDVTRAQLLRAMPGLKERAKTLIELIEGARFIFADRPLPIEAKAAALLTPETRALIDRLRAALESVTSWNAETTEAAMRTFAEQNNLKLGAIAQPLRIALTGRTTSPGIFDVLAALGKETCLARLGDQGSR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-07-25" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRK3</accession>
    <name>QUEF_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADPH-dependent 7-cyano-7-deazaguanine reductase</fullName>
            <ecNumber evidence="1">1.7.1.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">7-cyano-7-carbaguanine reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">NADPH-dependent nitrile oxidoreductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">PreQ(0) reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">queF</name>
        <name type="ordered locus">Nwi_1828</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7-aminomethyl-7-carbaguanine + 2 NADP(+) = 7-cyano-7-deazaguanine + 3 H(+) + 2 NADPH</text>
            <dbReference type="Rhea" id="RHEA:13409"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:45075"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58703"/>
            <dbReference type="EC" id="1.7.1.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; tRNA-queuosine biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.7.1.13" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315084.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRK3"/>
    <dbReference type="SMR" id="Q3SRK3"/>
    <dbReference type="STRING" id="323098.Nwi_1828"/>
    <dbReference type="EnsemblBacteria" id="ABA05088">
        <property type="protein sequence ID" value="ABA05088"/>
        <property type="gene ID" value="Nwi_1828"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1828"/>
    <dbReference type="eggNOG" id="COG0780">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102489_0_1_5"/>
    <dbReference type="OMA" id="LECKEFT"/>
    <dbReference type="OrthoDB" id="1675155at2"/>
    <dbReference type="UniPathway" id="UPA00392"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046857">
        <property type="term" value="F:oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033739">
        <property type="term" value="F:preQ1 synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008616">
        <property type="term" value="P:queuosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00818">
        <property type="entry name" value="QueF_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029500">
        <property type="entry name" value="QueF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016856">
        <property type="entry name" value="QueF_type1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14489">
        <property type="entry name" value="QueF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03139">
        <property type="entry name" value="QueF-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0671">Queuosine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000247683" description="NADPH-dependent 7-cyano-7-deazaguanine reductase">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="active site" description="Thioimide intermediate" evidence="1">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="78"/>
            <end position="80"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="97"/>
            <end position="98"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00818"/>
        </source>
    </evidence>
    <sequence length="158" mass="17507" checksum="2103BCC3623A2087" modified="2005-10-11" version="1">MPRKPPSSPKSSPLQLGRAVAWPDSPEQAKLDRVPNPQKGTNYVARFTAPEFTALCPVTGQPDFAHLVIDYVPGSWLLESKSLKLYLASFRNHGAFHEDCTVAIGKRIVAAIKPKWLRIGGYWFPRGGIPIDVFWQTGAAPRGVWIPDQDVPSYRGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRL2</accession>
    <name>Q3SRL2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Pyruvate dehydrogenase E1 component subunit alpha</fullName>
            <ecNumber evidence="3 8">1.2.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pdhA</name>
        <name evidence="11" type="ordered locus">Nwi_1818</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>(R)-N(6)-lipoyl-L-lysyl-[dihydrolipoyllysine-residue acetyltransferase] + H(+) + pyruvate = (R)-N(6)-(S(8)-acetyldihydrolipoyl)-L-lysyl-[dihydrolipoyllysine-residue acetyltransferase] + CO2</text>
            <dbReference type="Rhea" id="RHEA:19189"/>
            <dbReference type="Rhea" id="RHEA-COMP:10480"/>
            <dbReference type="Rhea" id="RHEA-COMP:10481"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="1.2.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2 8">Heterodimer of an alpha and a beta chain.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.1" evidence="3 8"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1818"/>
    <dbReference type="EnsemblBacteria" id="ABA05079">
        <property type="protein sequence ID" value="ABA05079"/>
        <property type="gene ID" value="Nwi_1818"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1818"/>
    <dbReference type="eggNOG" id="COG1071">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029393_5_2_5"/>
    <dbReference type="OMA" id="LGYEMPC"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043231">
        <property type="term" value="C:intracellular membrane-bounded organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:acetyl-CoA biosynthetic process from pyruvate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017597">
        <property type="entry name" value="Pyrv_DH_E1_asu_subgrp-y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03182">
        <property type="entry name" value="PDH_E1_alph_y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 8" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="E1_dh" evidence="10">
        <location>
            <begin position="37"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="321"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA05079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="342" mass="37835" checksum="DBF7A3519DFFB724" modified="2005-10-11" version="1">MTMVASKKNVAQAAGSGSKSQPSPSFTREQDLHALREMLLIRRFEEKAGQLYGMGAIGGFCHLYIGQEAIVVGMQMALGEGDQVITGYRDHGHMLACGMDAKGVMAELTGRRGGYSRGKGGSMHMFSKEKHFYGGHGIVGAQVSLGTGLAFANRYRGNDRVSLTYFGDGAANQGQVYESFNMAQLWKLPVVYIIENNRYAMGTSVARSSAQIDFSRRGVSFDIPGEQVDGMDVRAVKAAGDKAVKWCREGNGPYILEMQTYRYRGHSMSDPAKYRTREEVDKVRHDQDPIEQVRNRLLASKVSEDELKKIDAKVREIINTAADFAQNDPEPDASELYTDIYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRN0</accession>
    <name>Q3SRN0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1800</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315057.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05061">
        <property type="protein sequence ID" value="ABA05061"/>
        <property type="gene ID" value="Nwi_1800"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1800"/>
    <dbReference type="eggNOG" id="ENOG50321WS">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_187653_0_0_5"/>
    <dbReference type="OMA" id="QAWPNFE"/>
    <dbReference type="OrthoDB" id="1998465at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229277" evidence="1">
        <location>
            <begin position="31"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="84" mass="8640" checksum="9B36CAE8F8DBE66D" modified="2005-10-11" version="1">MIKALSAVAVAAFIAAALTVLPGFAPQVSASTPVPLAKADRLDIRPAGAVCSQQAWPNFDASCLRAAGSRTVVREARLITAGHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRQ8</accession>
    <name>Q3SRQ8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Peptidase M23B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1772</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315029.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1772"/>
    <dbReference type="EnsemblBacteria" id="ABA05033">
        <property type="protein sequence ID" value="ABA05033"/>
        <property type="gene ID" value="Nwi_1772"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1772"/>
    <dbReference type="eggNOG" id="COG0739">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1388">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029425_0_0_5"/>
    <dbReference type="OMA" id="ANGYKGP"/>
    <dbReference type="OrthoDB" id="1891666at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016047">
        <property type="entry name" value="Peptidase_M23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01551">
        <property type="entry name" value="Peptidase_M23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51261">
        <property type="entry name" value="SSF51261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54106">
        <property type="entry name" value="SSF54106"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LysM" evidence="2">
        <location>
            <begin position="142"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="LysM" evidence="2">
        <location>
            <begin position="213"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="453" mass="46958" checksum="04B4BF58DCDFE1A8" modified="2005-10-11" version="1">MSRVVELLCSRRVPQVAVLALVSIGFAGCSADMSTRLAQLDSGPQQSNRFGWQRETTGTVAAAPAAPVERRELHQYSRPPYRPHSDYQSQPLPPISTPKSYPVASSVSVSGRGPGSYTPPARAIESAPAVAARDGWNHKGGTPIIVGTSDTLEGLSRRYGVSSAEILKANGYRGPRQLQPGQRLIIPKRAAVAETQPAPAAKPAVVATAASGATHHVNRGDTLMNIARRNHISVGELARANGLPPSAKLKIGTRITVPTKVAAVSAQPVAAPAAPPAVAPKPAPAPVVHAAAATAGPVQKVNLAAATATPEAAVVKPSVKAAEATGALPTFRWPVRGRVITAYGARTNGKSNDGINVAVPEGTPVKAAEDGVVAYAGNELKGYGNLVLVRHSNGYVTAYAHARELMVKRGDSIRRGQVIAKSGQSGEAGSPQLHFEIRKGSTPVDPLNFLNGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRW7</accession>
    <name>Q3SRW7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribonuclease R</fullName>
            <shortName evidence="4">RNase R</shortName>
            <ecNumber evidence="4">3.1.13.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rnr</name>
        <name evidence="7" type="ordered locus">Nwi_1713</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">3'-5' exoribonuclease that releases 5'-nucleoside monophosphates and is involved in maturation of structured RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.13.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RNR ribonuclease family. RNase R subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.1" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314972.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1713"/>
    <dbReference type="EnsemblBacteria" id="ABA04974">
        <property type="protein sequence ID" value="ABA04974"/>
        <property type="gene ID" value="Nwi_1713"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1713"/>
    <dbReference type="eggNOG" id="COG0557">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002333_7_1_5"/>
    <dbReference type="OMA" id="DWYEYRS"/>
    <dbReference type="OrthoDB" id="1602988at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008859">
        <property type="term" value="F:exoribonuclease II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01895">
        <property type="entry name" value="RNase_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040476">
        <property type="entry name" value="CSD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001900">
        <property type="entry name" value="RNase_II/R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022966">
        <property type="entry name" value="RNase_II/R_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004476">
        <property type="entry name" value="RNase_II/RNase_R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011805">
        <property type="entry name" value="RNase_R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17876">
        <property type="entry name" value="CSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00773">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00955">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00358">
        <property type="entry name" value="3_prime_RNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02063">
        <property type="entry name" value="RNase_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01175">
        <property type="entry name" value="RIBONUCLEASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="2 4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
            <begin position="640"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="727"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="744"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="759"/>
            <end position="782"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="782" mass="85737" checksum="67A512931787885E" modified="2005-10-11" version="1">MSKPRARNFPDREAIVAFIRAHPGRAGTREIAHEFGLKNADRAELKHILRALADDGTIVKRSRKLRKPETLPPTLVADITGRDRDGELIATPTEWDDEATPAPRILIHVPRRAKPGTVAGVGDRALLRIETIESDETASYRARVIRVIDRTRARMLGIYRASPQGGGRLVPVDKKQAGRELNIAKADSAGAEDGDLVSVELVRTRGFGLASGKVKERLGSLATEKAVSLIAIHAHDIPQEFTPAALREAAAAKPSALQGREDWRAVPLITIDPPDAKDHDDAVHAAPDDDPNNTGGAIVTVAIADVAYYVRPESALDREALERGNSVYFPDRVVPMLPERISNDLCSLVPGEARGALAVRMVIDADGRKRSHTFHRILMRSAAKLAYSQVQAAIDGNPDDATAPLLEPILKPLYAAFALVKRARDERDPLDLELPERKILLKKDGTVDRVIVPDRLDAHRLIEEFMILANVAAAEMLEKKGLPLIYRVHDEPTVEKVHNLREFLKTLDMSFARSGALRPELFNRVLDQVRGHDSESLVNEVVLRSQAQAEYSAENYGHFGLNLRRYAHFTSPIRRYADLVVHRALLRALGLGDGALPDSDNAETLAEIAARISVTERRAMKAERETADRLIAHFLADRIGNIFEARISGVTRAGLFVKLADTGADGLIPIRSLGTEYFKYDETRHALVGSRSGSMHKLGDVVEVRLVEAVPLAGALRFELLSGAEVRPRSPRARGGGKIKTPRGRNAGHPDVHSKAHPGRKPRGTSRKPGRSKAGKPKKGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="134" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRW8</accession>
    <name>Q3SRW8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA topoisomerase 1</fullName>
            <ecNumber evidence="10">5.6.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">DNA topoisomerase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">topA</name>
        <name evidence="13" type="ordered locus">Nwi_1712</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the type IA topoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="10"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314971.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1712"/>
    <dbReference type="EnsemblBacteria" id="ABA04973">
        <property type="protein sequence ID" value="ABA04973"/>
        <property type="gene ID" value="Nwi_1712"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1712"/>
    <dbReference type="eggNOG" id="COG0550">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1754">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002929_0_2_5"/>
    <dbReference type="OMA" id="PECKYTR"/>
    <dbReference type="OrthoDB" id="223233at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00186">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03363">
        <property type="entry name" value="TOPRIM_TopoIA_TopoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00952">
        <property type="entry name" value="Topoisom_1_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000380">
        <property type="entry name" value="Topo_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003601">
        <property type="entry name" value="Topo_IA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023406">
        <property type="entry name" value="Topo_IA_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013497">
        <property type="entry name" value="Topo_IA_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013824">
        <property type="entry name" value="Topo_IA_cen_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013825">
        <property type="entry name" value="Topo_IA_cen_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013826">
        <property type="entry name" value="Topo_IA_cen_sub3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023405">
        <property type="entry name" value="Topo_IA_core_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003602">
        <property type="entry name" value="Topo_IA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013498">
        <property type="entry name" value="Topo_IA_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005733">
        <property type="entry name" value="TopoI_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028612">
        <property type="entry name" value="Topoisom_1_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025589">
        <property type="entry name" value="Toprim_C_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034149">
        <property type="entry name" value="TOPRIM_TopoI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42785">
        <property type="entry name" value="PTHR42785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01131">
        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13368">
        <property type="entry name" value="Toprim_C_rpt"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01396">
        <property type="entry name" value="zf-C4_Topoisom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00417">
        <property type="entry name" value="PRTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00437">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00436">
        <property type="entry name" value="TOP1Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56712">
        <property type="entry name" value="SSF56712"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01051">
        <property type="entry name" value="topA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00396">
        <property type="entry name" value="TOPOISOMERASE_I_PROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 10 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0799">Topoisomerase</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Toprim" evidence="12">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with DNA" evidence="10">
        <location>
            <begin position="165"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="675"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="830"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="845"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="10">
        <location>
            <position position="303"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="31"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="305"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA04973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="911" mass="99494" checksum="7E6A1D92370F0281" modified="2005-10-11" version="1">MNIVIVESPAKAKTINKYLGTSYEVLASFGHVRDLPAKNGSVDPDANFQMIWEVDPKAAGRLNDIAKSLKGADKLILATDPDREGEAISWHVLEVLKQKRALKDQKIERVVFNAITKQAVTEAMKSPRQIDGALVDAYMARRALDYLVGFTLSPVLWRKLPGARSAGRVQSVALRLVCDRELEIEKFVPREYWSLVTTLATPRGETFDARLVGADGKKIQRLDIGNGAEAEDFRKAIEAANFTVSTVEAKPARRNPQAPFTTSTLQQEASRKLGFAPAHTMRIAQRLYEGIDVGGETTGLITYMRTDGVQIDGSAITQARKVIGEDYGHAYVPEAPRQYQTKAKNAQEAHEAIRPTDLSRRPSDMKRRLDADQARLYELIWIRTIASQMESAELERTTVDIAARAGARTLELRASGQVVKFDGFLALYQEGRDDEEDEDSRRLPAMSEGEALRRENLSVTQHFTEPPPRFSEASLVKRMEELGIGRPSTYASILQVLKDRGYVKLEKKRLYGEDKGRVVIAFLENFFARYVEYDFTANLEEQLDRISNNEVSWQQVLKDFWDDFIGVVNEIKDVRVSEVLDVLDNMLGPHIYAPREDGGDPRQCPTCGTGRLNLKAGKFGAFVGCSNYPECRHTRPLAADGEAGGDRVLGKDPESGLDVAVKSGRFGPYIQLGEASDYGEGEKPKRAGIPKNTSPADIELDLAVKLLSLPREIGKHPETGEPITAGIGRFGPFVRHEKTYASLEAGDEVFDIGLNRAVTLIAEKVAKGPSRRFGADPGKPLGDHPTLGPVAVKSGRYGAYVTAGGVNATIPSDKTQDTITLPEAIALIDERAAKGGGKPKRAAKKAKPAQTEKAGKDGAATKKPAKKAASKQASTKPKSEAVSKARGSIAAAKAPPNTVKAPTKKSAGKNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRX5</accession>
    <name>Q3SRX5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1705</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04966">
        <property type="protein sequence ID" value="ABA04966"/>
        <property type="gene ID" value="Nwi_1705"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1705"/>
    <dbReference type="eggNOG" id="ENOG5033F1X">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_196550_0_0_5"/>
    <dbReference type="OMA" id="ENVYFTA"/>
    <dbReference type="OrthoDB" id="1974428at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="63" mass="6963" checksum="2191C97F20A67B06" modified="2005-10-11" version="1">MKHKFLVGETVLFTASNVARPAASGTYEVIRLLPTDGDDFQYRIKNSAEAFERVAKESQLAAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS57</accession>
    <name>Q3SS57_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1623</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1623"/>
    <dbReference type="EnsemblBacteria" id="ABA04884">
        <property type="protein sequence ID" value="ABA04884"/>
        <property type="gene ID" value="Nwi_1623"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1623"/>
    <dbReference type="HOGENOM" id="CLU_2302943_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="103" mass="11773" checksum="78A5691E7E9CDF27" modified="2005-10-11" version="1">MTMQNDEKHDRQLWCAVLGQAITDATTTRTGKDAQRDQHAAIQWLLTDTIDFPIVCTLAGVDPEAVRERFMRTPGVVEKFQGTQRDRRGSRTHDLTELGFFPR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-28" modified="2022-05-25" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS83</accession>
    <name>PUR5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribosylformylglycinamidine cyclo-ligase</fullName>
            <ecNumber evidence="1">6.3.3.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">AIR synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">AIRS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphoribosyl-aminoimidazole synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purM</name>
        <name type="ordered locus">Nwi_1597</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ATP = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AIR synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.1" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314862.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SS83"/>
    <dbReference type="SMR" id="Q3SS83"/>
    <dbReference type="STRING" id="323098.Nwi_1597"/>
    <dbReference type="EnsemblBacteria" id="ABA04858">
        <property type="protein sequence ID" value="ABA04858"/>
        <property type="gene ID" value="Nwi_1597"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1597"/>
    <dbReference type="eggNOG" id="COG0150">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047116_0_0_5"/>
    <dbReference type="OMA" id="EPLFMTD"/>
    <dbReference type="OrthoDB" id="923547at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00129"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004641">
        <property type="term" value="F:phosphoribosylformylglycinamidine cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02196">
        <property type="entry name" value="PurM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00741">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004733">
        <property type="entry name" value="PurM_cligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520">
        <property type="entry name" value="PTHR10520"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00878">
        <property type="entry name" value="purM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000148289" description="Phosphoribosylformylglycinamidine cyclo-ligase">
        <location>
            <begin position="1"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00741"/>
        </source>
    </evidence>
    <sequence length="357" mass="36684" checksum="0F0D990C6CE2E40A" modified="2005-10-11" version="1">MTDRNISLTYAGAGVDIDAGNRLVDLIKPMVRATARAGAEAEIGGFGGLFDLKAAGFADPVLVAANDGVGTKVKIAIETGIHDTIGIDLVAMSVNDLVVQGAEPLFFLDYFACGKLDPETAAAIVAGIAEGCREAGCALIGGETAEMPGLYKDGDYDLAGFAVGAAERGSLLPGRDIAAGDAVIGLASSGVHSNGYSLVRNIVEASGIALSAPAPFAPVATLGGALLTPTRLYVKSCLRAIRETGAVKGLAHITGGGFTDNIPRVLPEGLGVRIDLAGLTVLPVFKWLAQQGRVVEREMLRTFNCGIGMVAIVRRDAVQQVVDVLTQAGERVSLLGDVIVAGDEGRVVYDGQLDLAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSB3</accession>
    <name>Q3SSB3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative outer-membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1567</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314834.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1567"/>
    <dbReference type="EnsemblBacteria" id="ABA04828">
        <property type="protein sequence ID" value="ABA04828"/>
        <property type="gene ID" value="Nwi_1567"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1567"/>
    <dbReference type="eggNOG" id="COG3637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037100_0_1_5"/>
    <dbReference type="OMA" id="QASATRW"/>
    <dbReference type="OrthoDB" id="2018542at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027385">
        <property type="entry name" value="OMP_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13505">
        <property type="entry name" value="OMP_b-brl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229161" evidence="1">
        <location>
            <begin position="25"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="OMP_b-brl" evidence="2">
        <location>
            <begin position="24"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="258" mass="27199" checksum="7A6CCB16A9A597C2" modified="2005-10-11" version="1">MSKLLIGAVSALAIGLGAVAPAAAADLAPRPYTKAPPPMMAAIYDWSGFYIGANGGWGSSRNCWDFTTPAGVFVANEGCHNATGGTAGGQIGYRWQATNWLFGLEAQGNWADLSGRNVSLAFPAFTNNSNLNAFGLFTGQIGYAWDATLLYVKGGAAVTSNRYRSLVTATGVQATDSVNNNRWGAVVGVGLEYGFAPNWSLAFEYDHIFMGNSRHTFTNNGVAGAAGTLFGTDNISQDVDIVTARINYRWGGPAIARY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSC0</accession>
    <name>Q3SSC0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1560</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04821">
        <property type="protein sequence ID" value="ABA04821"/>
        <property type="gene ID" value="Nwi_1560"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1560"/>
    <dbReference type="HOGENOM" id="CLU_2437841_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="90" mass="9933" checksum="FA35DFCEC8FB8B2A" modified="2005-10-11" version="1">MKNEVLQGLIDPDRVPDLFAEGFASIEKAGNACVRFTLYARRNYGTEIEHIVIGRVVIPKEILPTAYRQTRAVLEGGPFLSAVPIASFLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSC1</accession>
    <name>Q3SSC1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1559</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314826.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04820">
        <property type="protein sequence ID" value="ABA04820"/>
        <property type="gene ID" value="Nwi_1559"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1559"/>
    <dbReference type="HOGENOM" id="CLU_1935821_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="130" mass="14618" checksum="F12826598B7645BD" modified="2005-10-11" version="1">MTRPSPTGAKLSKTGADHSIGKYRSIDRWFSRVVDALRREHPDKLAQTLADYAHRPQRVCELWLAGRQSPNGEALASLLRSPIGGIVLDAITAGAEAEWIARDRRARRLLTIHEREAELRREKAQALEDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSD2</accession>
    <name>Q3SSD2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1548</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1548"/>
    <dbReference type="EnsemblBacteria" id="ABA04809">
        <property type="protein sequence ID" value="ABA04809"/>
        <property type="gene ID" value="Nwi_1548"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1548"/>
    <dbReference type="eggNOG" id="COG1403">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1957278_0_0_5"/>
    <dbReference type="OMA" id="WSKARDT"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="128" mass="14534" checksum="CE132CF887432945" modified="2005-10-11" version="1">MSMPTVSDYDARRGSARSRGYDGRWDRAAKQFKWKHPLCLGCKAEGRVTPTAVVDHVVPHRGDMVLFWDQDRWQSSCDFHHNVVKQQLEHMFDRGALPEAELWLDSATAVAISRRERAVIGVDGWVGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSF6</accession>
    <name>Q3SSF6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RND efflux system, outer membrane lipoprotein, NodT</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1524</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the outer membrane factor (OMF) (TC 1.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1524"/>
    <dbReference type="EnsemblBacteria" id="ABA04785">
        <property type="protein sequence ID" value="ABA04785"/>
        <property type="gene ID" value="Nwi_1524"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1524"/>
    <dbReference type="eggNOG" id="COG1538">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012817_13_1_5"/>
    <dbReference type="OMA" id="NFMVTNL"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003423">
        <property type="entry name" value="OMP_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010131">
        <property type="entry name" value="RND_efflux_OM_lipoprot_NodT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02321">
        <property type="entry name" value="OEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01845">
        <property type="entry name" value="outer_NodT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0564">Palmitate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="201"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="494" mass="53199" checksum="62207AD81188920E" modified="2005-10-11" version="1">MSAPAARALGHPLRRATWIRRSAMLLPLWAAVAASAGCILTRDIPDPALDIPMAYKAASTTAADALPALDWWRGFRSSELTSLMEEAQTVNLDIAAAAARIIQADRQARIIGAALLPSVSIGGQESYFRTSGSSQSGLTNRGREVVNYSGTLSGASYELDFWGKNRDATLAALETANASRFDRDVVALTTLVSVANAYFQVLTAQDRIRTAERNIASARRILDALKQRLSAGTGSDLDVAQQESVLANLRATVPQLRQTLVQNINALATLVARPPESVTVKGGSLNRITAPRVTPGLPSELLTQRPDIRRQELQLASATANVGSARAQFFPSIQLTAQGGYQSPALVALFAPNAAFFNLAGALTQPIFDGRRILSNFELTKARQDELLQTYRKTVISAFADVGNALTAVRQTTERLRLQREVVAASRKAFQLSEQQLQAGTADIIIVLNTQLTLFQAEDVLSQAQLARLQAIVSLYQALGGGWEPRIERAGNAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSG0</accession>
    <name>Q3SSG0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA-uridine aminocarboxypropyltransferase</fullName>
            <ecNumber evidence="1">2.5.1.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_1520</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.5.1.25" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314787.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1520"/>
    <dbReference type="EnsemblBacteria" id="ABA04781">
        <property type="protein sequence ID" value="ABA04781"/>
        <property type="gene ID" value="Nwi_1520"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1520"/>
    <dbReference type="eggNOG" id="COG3148">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066458_2_0_5"/>
    <dbReference type="OMA" id="HTLCKYQ"/>
    <dbReference type="OrthoDB" id="1719529at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016432">
        <property type="term" value="F:tRNA-uridine aminocarboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005636">
        <property type="entry name" value="DTW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039262">
        <property type="entry name" value="DTWD2/YfiP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21392">
        <property type="entry name" value="PTHR21392"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03942">
        <property type="entry name" value="DTW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01144">
        <property type="entry name" value="DTW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DTW" evidence="5">
        <location>
            <begin position="14"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA04781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="237" mass="26482" checksum="42544BD4206C7D10" modified="2005-10-11" version="1">MSSNSESSAAESEALPQCGRCHKAEVLCVCGSIEQIKNRTALLLLQHPQEQDKALGTARLTALHVENAVLKIGLSWPSLSRALGRPVHDPSRWAALYLGSVRADRFNTDRTIVAIDRNGQMKTDQRAALADIEGVVLLDGTWSQAKALWWRNPWLLKCQRVVLAPGRASRYGKLRKEPRRDGLATIEAAAIMLAGLEDRPDIETTLLASFDRMLTKYQEAQADMPELATRPRRRRHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSI1</accession>
    <name>Q3SSI1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF2147 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1499</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1499"/>
    <dbReference type="EnsemblBacteria" id="ABA04760">
        <property type="protein sequence ID" value="ABA04760"/>
        <property type="gene ID" value="Nwi_1499"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1499"/>
    <dbReference type="eggNOG" id="COG4731">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_983106_0_0_5"/>
    <dbReference type="OMA" id="CGGQTWT"/>
    <dbReference type="OrthoDB" id="1848240at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019223">
        <property type="entry name" value="DUF2147"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017201">
        <property type="entry name" value="UCP037429"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09917">
        <property type="entry name" value="DUF2147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037429">
        <property type="entry name" value="UCP037429"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229167" description="DUF2147 domain-containing protein" evidence="2">
        <location>
            <begin position="29"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2147" evidence="3">
        <location>
            <begin position="176"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="272" mass="28014" checksum="1067ABE1F2AD0F21" modified="2005-10-11" version="1">MNFRTGVDPMKALGVAAALLLTLSTAQAGENFSFEIGGHTVRIHASRGCDSPSCISISIPGLYNSKRKRFDDADSGDSDVPQAGSVQAPAASSPSAPPPAPAQASATASAPAPAATAPTPAQQPSPSIAVSTPTASTGAPVEPVPSQDTKTVASNVPAAEPPEASPRATEPAGPLGMWVTEKKEGRVRIEECGKNLCGYSVDSRSGRNKEKILINMKPSGSKWSGKIYDPNSGSTYSSTVAMKGKDRLRVQGCAFGGMFCGGQIWTRLNQSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSK0</accession>
    <name>Q3SSK0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HNH endonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1480</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1480"/>
    <dbReference type="EnsemblBacteria" id="ABA04741">
        <property type="protein sequence ID" value="ABA04741"/>
        <property type="gene ID" value="Nwi_1480"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1480"/>
    <dbReference type="eggNOG" id="COG1403">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108879_4_0_5"/>
    <dbReference type="OMA" id="KPPRICG"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00085">
        <property type="entry name" value="HNHc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038575">
        <property type="entry name" value="E6_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002711">
        <property type="entry name" value="HNH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003615">
        <property type="entry name" value="HNH_nuc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01844">
        <property type="entry name" value="HNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00507">
        <property type="entry name" value="HNHc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161229">
        <property type="entry name" value="SSF161229"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HNHc" evidence="1">
        <location>
            <begin position="60"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="120" mass="14100" checksum="E0A475C7DDFC171F" modified="2005-10-11" version="1">MRLPYPKLGMPMPFRAPRICSCGLKVAYGVRCVCQQRTRAEADKRRPNARERGYDNTWQKARSYFLDKHPRCVMCGAAARVVDHIRPHRGDTKLFWDRGNWQSLCFSCHNSRKQRMERAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SST0</accession>
    <name>Q3SST0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Type III secretion protein, related to flagellar biosynthesis protein FlhB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type III secretion exporter family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314670.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1400"/>
    <dbReference type="EnsemblBacteria" id="ABA04661">
        <property type="protein sequence ID" value="ABA04661"/>
        <property type="gene ID" value="Nwi_1400"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1400"/>
    <dbReference type="eggNOG" id="COG2257">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041013_4_2_5"/>
    <dbReference type="OMA" id="HEVPIYE"/>
    <dbReference type="OrthoDB" id="1823885at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1690.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006135">
        <property type="entry name" value="T3SS_substrate_exporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029025">
        <property type="entry name" value="T3SS_substrate_exporter_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30531">
        <property type="entry name" value="PTHR30531"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01312">
        <property type="entry name" value="Bac_export_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160544">
        <property type="entry name" value="SSF160544"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0282">Flagellum</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="89" mass="9519" checksum="0EC4824AAAE3A2DC" modified="2005-10-11" version="1">MSVETRDQVAVALHYDKGSGAPRVVAKGRGSLGAKIIEVANEHDIPIEQNEVLADALSHVELGDEIPAELYKAVAEVLVFVLRMSGRAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSZ6</accession>
    <name>Q3SSZ6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Response regulator receiver</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1334</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314613.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1334"/>
    <dbReference type="EnsemblBacteria" id="ABA04595">
        <property type="protein sequence ID" value="ABA04595"/>
        <property type="gene ID" value="Nwi_1334"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1334"/>
    <dbReference type="eggNOG" id="COG0784">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_12_5"/>
    <dbReference type="OMA" id="FAPIIML"/>
    <dbReference type="OrthoDB" id="1630092at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="10"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="141"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="181" mass="20574" checksum="A5BE941F059C7232" modified="2005-10-11" version="1">MYGIDFNKLRFLVCDDNAHMRRILRTLLHSFGAREVYEAEDGATALEMYTHYVPDIVIVDWAMPIFDGIELTQMIRQPDSKGNPFAPIIMLTGHSEKRRVTVARDAGITEFLAKPISAKGLYQRILNVVVSPRPFIKTKNYFGPDRRRNTSNAYIGPERRGAREPDVIQQPSLLDKARAPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST07</accession>
    <name>Q3ST07_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ISCc1, transposase OrfB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1323</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1323"/>
    <dbReference type="EnsemblBacteria" id="ABA04584">
        <property type="protein sequence ID" value="ABA04584"/>
        <property type="gene ID" value="Nwi_1323"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1323"/>
    <dbReference type="eggNOG" id="COG3385">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055261_9_3_5"/>
    <dbReference type="OMA" id="ECAFCRL"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025668">
        <property type="entry name" value="Tnp_DDE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13586">
        <property type="entry name" value="DDE_Tnp_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tnp_DDE_dom" evidence="1">
        <location>
            <begin position="16"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="101" mass="11509" checksum="703DFA19C97975B2" modified="2005-10-11" version="1">MTQAPALLRGQTGEAVLADKAYNSNALRMLIAGMGATAVIPSNRTRKVIIPHDATAYRQRNRIERCFCHLKHFRRFATRYDRRSAHFAGFIHLAAAMIWLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST25</accession>
    <name>Q3ST25_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphoribosylformylglycinamidine synthase subunit PurS</fullName>
            <shortName evidence="5">FGAM synthase</shortName>
            <ecNumber evidence="5">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Formylglycinamide ribonucleotide amidotransferase subunit III</fullName>
            <shortName evidence="5">FGAR amidotransferase III</shortName>
            <shortName evidence="5">FGAR-AT III</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Phosphoribosylformylglycinamidine synthase subunit III</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">purS</name>
        <name evidence="6" type="ordered locus">Nwi_1305</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + H2O + L-glutamine + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ADP + H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PurS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314586.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1305"/>
    <dbReference type="EnsemblBacteria" id="ABA04566">
        <property type="protein sequence ID" value="ABA04566"/>
        <property type="gene ID" value="Nwi_1305"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1305"/>
    <dbReference type="eggNOG" id="COG1828">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_164833_3_0_5"/>
    <dbReference type="OMA" id="IENYRFE"/>
    <dbReference type="OrthoDB" id="2012814at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01926">
        <property type="entry name" value="PurS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003850">
        <property type="entry name" value="PurS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036604">
        <property type="entry name" value="PurS-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34696">
        <property type="entry name" value="PTHR34696"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02700">
        <property type="entry name" value="PurS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82697">
        <property type="entry name" value="SSF82697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00302">
        <property type="entry name" value="TIGR00302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 5" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA04566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="79" mass="8251" checksum="FC2587BCC6569778" modified="2005-10-11" version="1">MKARVTVTLKAGVLDPQGKAIEGALKSLGVDGIASVRQGKVFDIELDGDKTKAEAALKAAADKLLANTVIENYRVEVLG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST46</accession>
    <name>GCSP_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycine dehydrogenase (decarboxylating)</fullName>
            <ecNumber evidence="1">1.4.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glycine cleavage system P-protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glycine decarboxylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glycine dehydrogenase (aminomethyl-transferring)</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gcvP</name>
        <name type="ordered locus">Nwi_1284</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-N(6)-lipoyl-L-lysyl-[glycine-cleavage complex H protein] + glycine + H(+) = (R)-N(6)-(S(8)-aminomethyldihydrolipoyl)-L-lysyl-[glycine-cleavage complex H protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:24304"/>
            <dbReference type="Rhea" id="RHEA-COMP:10494"/>
            <dbReference type="Rhea" id="RHEA-COMP:10495"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="1.4.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GcvP family.</text>
    </comment>
    <dbReference type="EC" id="1.4.4.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314571.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ST46"/>
    <dbReference type="SMR" id="Q3ST46"/>
    <dbReference type="STRING" id="323098.Nwi_1284"/>
    <dbReference type="EnsemblBacteria" id="ABA04545">
        <property type="protein sequence ID" value="ABA04545"/>
        <property type="gene ID" value="Nwi_1284"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1284"/>
    <dbReference type="eggNOG" id="COG0403">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1003">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004620_3_2_5"/>
    <dbReference type="OMA" id="CVPMSEY"/>
    <dbReference type="OrthoDB" id="70707at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004375">
        <property type="term" value="F:glycine dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00613">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00711">
        <property type="entry name" value="GcvP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003437">
        <property type="entry name" value="GcvP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020581">
        <property type="entry name" value="GDC_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11773">
        <property type="entry name" value="PTHR11773"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02347">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00461">
        <property type="entry name" value="gcvP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000227109" description="Glycine dehydrogenase (decarboxylating)">
        <location>
            <begin position="1"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="699"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00711"/>
        </source>
    </evidence>
    <sequence length="954" mass="102794" checksum="24D9EE73A28C9B9A" modified="2005-10-11" version="1">MIAREEPATTFARRHIGPSSRDIAAMLETVGAKSLEALMNEALPPSIRQKTPLDLGEGFSETEVLTHMQALAAQNQPLTSLIGQGYSGTILPAVIQRNILENPAWYTAYTPYQPEISQGRLEALFNFQTMICDLTGLDVANASLLDEATAAAEAMALAERASSVKTKAFFVDHEVHPQTLAVLRTRAEPLGWTLVTGDPLHDLDKADVFGAVLQYPGTSGVVRDLRPAIAAIKAKGGLAVVAADLLALTLLTSPGVLGADIAIGSAQRFGVPMGYGGPHAAYMAVCNALKRLLPGRLVGLSVDSRGTPAYRLALQTREQHIRREKATSNICTAQVLLAVISSMYAVYHGPEGLAQIARTVHRHTATLAAGLTRLGFAPLNSTAFDTLTVNAGERQSEIVKRASSQGINLRINADGTLGIALDELTIEETVEALWRAFGATWSYADVEAHAPDLLPADLKRKTAYLTHPVFHEHRSETELLRYMRKLSDRDLALDRAMIPLGSCTMKLNATTEMIPLTWAAFANLHPFAPPEQAEGYFTLFENFEEWLLDITGYDAISLQPNSGAQGEYAGLLAIRGYHAARGESHRTVCLIPSSAHGTNPASANMAGMDVVVVACDARGDVDVDDLRTKSTQHADRLAAIMITYPSTHGVFEERIREICDIVHGHGGQVYLDGANMNAQVGLSRPGDYGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFLPGHPAIDDATPSAVGPVSAAPFGSASILTISYIYILMMGSEGLKRATEVAILNANYIAQRLDPHFPVLYRNVKGRVAHECIIDPRALKAKTGVTVDDIAKRLIDYGFHAPTMSFPVPGTLMIEPTESESKAELDRFCDAMIAIRQEIAEIEAGRWKVEASPLRHAPHTAHDIADDAWSRPYSRAQGCFPSGSSRSDKYWCPVGRVDNAYGDRNLVCSCPPVEDYAQAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STC4</accession>
    <name>Q3STC4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Malto-oligosyltrehalose trehalohydrolase</fullName>
            <shortName evidence="8">MTHase</shortName>
            <ecNumber evidence="3 8">3.2.1.141</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 8">4-alpha-D-((1-&gt;4)-alpha-D-glucano)trehalose trehalohydrolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6 8">Maltooligosyl trehalose trehalohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Nwi_1205</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1 8">Glycan biosynthesis; trehalose biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glycosyl hydrolase 13 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.141" evidence="3 8"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314496.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1205"/>
    <dbReference type="CAZy" id="GH13">
        <property type="family name" value="Glycoside Hydrolase Family 13"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04467">
        <property type="protein sequence ID" value="ABA04467"/>
        <property type="gene ID" value="Nwi_1205"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1205"/>
    <dbReference type="eggNOG" id="COG0296">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020726_0_0_5"/>
    <dbReference type="OMA" id="FTPMLFM"/>
    <dbReference type="OrthoDB" id="148706at2"/>
    <dbReference type="UniPathway" id="UPA00299"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033942">
        <property type="term" value="F:4-alpha-D-(1-&gt;4)-alpha-D-glucanotrehalose trehalohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022567">
        <property type="entry name" value="DUF3459"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="Glyco_hydro_13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004193">
        <property type="entry name" value="Glyco_hydro_13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012768">
        <property type="entry name" value="Trehalose_TreZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044901">
        <property type="entry name" value="Trehalose_TreZ_E-set_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02922">
        <property type="entry name" value="CBM_48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11941">
        <property type="entry name" value="DUF3459"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006337">
        <property type="entry name" value="Trehalose_TreZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="SSF81296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02402">
        <property type="entry name" value="trehalose_TreZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 8 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aamy" evidence="12">
        <location>
            <begin position="90"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="252"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="317"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="388"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="254"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9">
        <location>
            <position position="291"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="11">
        <location>
            <position position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR006337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR006337-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR006337-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR006337-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA04467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="594" mass="66957" checksum="2D5CE8E72694CAED" modified="2005-10-11" version="1">MSASRFGPALTPEGATFRLWAPAASKVAVVTDTAHRMEKDGSGWFVAEVTTAQAGTRYHYRIDDELDVPDPASSYQRDDVFGRSELVDHRRFRWKAIGWRGRPWHETVILECHVGAFTPEGTYRAMIDKLDHLVSTGITAIELMPLADFSGTRNWGYDGALWYAPDHVYGTPDDLKELIDQAHLRGLMVFLDVVYNHFGPEGNYLARYAPQFFCDAKTPWGAAIDYRVPEVRAFAVNNALHWLSNYRFDGLRLDAVHAIPETGEPPILADISRAIGILARQDRRHIHLVLENDDNRASLLDPSSGVPGGKYRAQWNDDYHHAWHVMLTQESHGYYGDYADNPRHLLARTLHAGFAYQGEPSAHRGGRARGERIGGLPPAAFVNFLQNHDQIGNRALGDRLEWSADARAIEAALAITLLAPMPPLMFMGEEWGATTPFPFFCDFKGDLADAVRAGRRREFAEAYDMFGDEVPDPLALSTFEAAVLDWPDQGKRPPRHVLVKRLLRIRRDELMPHLGNAKFASADLRQDDRLLIARWTLGNDARLHLRANISDHNVTDDTSPMQGRPIWGGEVPVRLPAWSVFWSLERPEEQRHSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STD4</accession>
    <name>Q3STD4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Periplasmic serine endoprotease DegP-like</fullName>
            <ecNumber evidence="5">3.4.21.107</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_1195</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>Acts on substrates that are at least partially unfolded. The cleavage site P1 residue is normally between a pair of hydrophobic residues, such as Val-|-Val.</text>
            <dbReference type="EC" id="3.4.21.107"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase S1C family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.107" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314486.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1195"/>
    <dbReference type="EnsemblBacteria" id="ABA04457">
        <property type="protein sequence ID" value="ABA04457"/>
        <property type="gene ID" value="Nwi_1195"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1195"/>
    <dbReference type="eggNOG" id="COG0265">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020120_1_0_5"/>
    <dbReference type="OMA" id="RALFQIQ"/>
    <dbReference type="OrthoDB" id="741829at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011782">
        <property type="entry name" value="Pept_S1C_Do"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001940">
        <property type="entry name" value="Peptidase_S1C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00834">
        <property type="entry name" value="PROTEASES2C"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="SSF50494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02037">
        <property type="entry name" value="degP_htrA_DO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="7">
        <location>
            <begin position="294"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="7">
        <location>
            <begin position="405"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="4">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="4">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="4">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR611782-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA04457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="516" mass="53013" checksum="0A35737B4E0A2ED5" modified="2005-10-11" version="1">MTDLPSSQLKPIRSGVTARRFALMASVVAGVGVAAYGLSPSADKAEFFISPAYAQVNTDVSKVAQPIGFADIVERVKPSVISVKIRMKDRAVASGDDSPFQPGSPMERFFRRFGGPDGLPPGMRGRRGPGLVTGQGSGFFISADGYAVTNNHVVDGADKVEVTTDDGKTYTAKVIGADPRTDIALIKVEGGSSFPFAKLSDDKVRIGDWVLAVGNPFGLGGSVTAGIVSASGRDIGTGPYDDFIQIDAPVNKGNSGGPAFNMKGDVIGVNTAIYSPSGGSVGIAFSIPANTVKSVVAQLKDKGSVSRGWIGVQIQPVTSDIADSLGLKKAEGALVAEPQANSPAAKAGIKSGDVITAVNGDPVKDARELARTIGSLAPGTSIKLNVLRKGTDKVVNLTLGTLPDTVEARADHYGDRGSSRDNDIPRLGLTVAPAGSVAGAGGEGVVVVDVDPKSPAAERGFREGDVILEVAGKSVSSSADIREAIKAARAEDKNSVLMRVRTGSTARFVAMPIAKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STJ4</accession>
    <name>Q3STJ4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Flagellar biosynthetic protein FliQ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">fliQ</name>
        <name evidence="10" type="ordered locus">Nwi_1135</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Role in flagellar biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Bacterial flagellum basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the FliQ/MopD/SpaQ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314428.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1135"/>
    <dbReference type="EnsemblBacteria" id="ABA04397">
        <property type="protein sequence ID" value="ABA04397"/>
        <property type="gene ID" value="Nwi_1135"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1135"/>
    <dbReference type="eggNOG" id="COG1987">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_164516_0_1_5"/>
    <dbReference type="OMA" id="TMPWMLN"/>
    <dbReference type="OrthoDB" id="1939288at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044780">
        <property type="term" value="P:bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002191">
        <property type="entry name" value="Bac_export_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006305">
        <property type="entry name" value="FliQ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34040">
        <property type="entry name" value="PTHR34040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01313">
        <property type="entry name" value="Bac_export_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004669">
        <property type="entry name" value="FliQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00952">
        <property type="entry name" value="TYPE3IMQPROT"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01402">
        <property type="entry name" value="fliQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="4 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="10" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="13"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA04397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="87" mass="9181" checksum="5C16F48C08F94B83" modified="2005-10-11" version="1">MTGAETLDVARDAIWTIVLVSGPLLGVGLIVGVAISLVQALTQIQEQTLVFVPKILAIFVTLVLALPFMADALHAEMLRISSRIIGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STP9</accession>
    <name>Q3STP9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CHRD protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1080</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314376.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1080"/>
    <dbReference type="EnsemblBacteria" id="ABA04342">
        <property type="protein sequence ID" value="ABA04342"/>
        <property type="gene ID" value="Nwi_1080"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1080"/>
    <dbReference type="eggNOG" id="COG2335">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107551_1_0_5"/>
    <dbReference type="OMA" id="LMAGQWY"/>
    <dbReference type="OrthoDB" id="1624298at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010895">
        <property type="entry name" value="CHRD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07452">
        <property type="entry name" value="CHRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00754">
        <property type="entry name" value="CHRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50933">
        <property type="entry name" value="CHRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229193" evidence="1">
        <location>
            <begin position="26"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="CHRD" evidence="2">
        <location>
            <begin position="24"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="141" mass="14251" checksum="21E59B14D8115670" modified="2005-10-11" version="1">MQANTGLLAVLTLAVAVTIAGQASAEKLKAMLNGASEVPPNTSKGSGTLDAHYDAASKKLAWKLTYSGLTGPVTAAHFHGPADVGQNAGIVVTIPEATSSPSEGSATLTDAQAADLLAGRYYVNVHTADHPGGEIRGQVTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STQ9</accession>
    <name>Q3STQ9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ADP-L-glycero-D-manno-heptose-6-epimerase</fullName>
            <ecNumber evidence="3">5.1.3.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">ADP-L-glycero-beta-D-manno-heptose-6-epimerase</fullName>
            <shortName evidence="3">ADP-glyceromanno-heptose 6-epimerase</shortName>
            <shortName evidence="3">ADP-hep 6-epimerase</shortName>
            <shortName evidence="3">AGME</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">hldD</name>
        <name evidence="5" type="ordered locus">Nwi_1070</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the interconversion between ADP-D-glycero-beta-D-manno-heptose and ADP-L-glycero-beta-D-manno-heptose via an epimerization at carbon 6 of the heptose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>ADP-D-glycero-beta-D-manno-heptose = ADP-L-glycero-beta-D-manno-heptose</text>
            <dbReference type="Rhea" id="RHEA:17577"/>
            <dbReference type="ChEBI" id="CHEBI:59967"/>
            <dbReference type="ChEBI" id="CHEBI:61506"/>
            <dbReference type="EC" id="5.1.3.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </cofactor>
        <text evidence="3">Binds 1 NADP(+) per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Nucleotide-sugar biosynthesis; ADP-L-glycero-beta-D-manno-heptose biosynthesis; ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate: step 4/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homopentamer.</text>
    </comment>
    <comment type="domain">
        <text evidence="3">Contains a large N-terminal NADP-binding domain, and a smaller C-terminal substrate-binding domain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NAD(P)-dependent epimerase/dehydratase family. HldD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.20" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314366.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1070"/>
    <dbReference type="EnsemblBacteria" id="ABA04332">
        <property type="protein sequence ID" value="ABA04332"/>
        <property type="gene ID" value="Nwi_1070"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1070"/>
    <dbReference type="eggNOG" id="COG0451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_3_5"/>
    <dbReference type="OMA" id="LRDFVYI"/>
    <dbReference type="OrthoDB" id="1180629at2"/>
    <dbReference type="UniPathway" id="UPA00356">
        <property type="reaction ID" value="UER00440"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008712">
        <property type="term" value="F:ADP-glyceromanno-heptose 6-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097171">
        <property type="term" value="P:ADP-L-glycero-beta-D-manno-heptose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05248">
        <property type="entry name" value="ADP_GME_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01601">
        <property type="entry name" value="Heptose_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011912">
        <property type="entry name" value="Heptose_epim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02197">
        <property type="entry name" value="heptose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1 3 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="4">
        <location>
            <begin position="3"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="205"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="10"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="31"/>
            <end position="32"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="71"/>
            <end position="75"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="326" mass="35666" checksum="71D94C70F1EC8559" modified="2005-10-11" version="1">MLLVTGGAGFIGSNLVAALNDAGRGDVVVCDALGHDGKWRNLAKRQLADVVPPAELTCWLDGRRLDAVFHLGAISETTATDGDLVIETNFRLSLRLLDWCAGTATPFIYASSASTYGDGAQGFRDDQSLAALRALRPMNLYGWSKHLFDMAVVGRAAQGGALPPQWAGLKFFNVFGPNEYHKGSMMSVLTRRFDDVKAGRPVQLFKSHRGGIADGDQRRDFIYVDDVVRVMMWLLATPSVSGLFNVGTGKARSFRDLMTAAYASLGARPNIEYIDMPEQIRGAYQYFTQADVARLQGAGYNGGFTPLEEAVDAYVKGYLDRDDRFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STY4</accession>
    <name>Q3STY4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Alanine dehydrogenase/PNT</fullName>
            <ecNumber evidence="5">1.6.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0995</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AlaDH/PNT family.</text>
    </comment>
    <dbReference type="EC" id="1.6.1.2" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314296.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0995"/>
    <dbReference type="EnsemblBacteria" id="ABA04257">
        <property type="protein sequence ID" value="ABA04257"/>
        <property type="gene ID" value="Nwi_0995"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0995"/>
    <dbReference type="eggNOG" id="COG3288">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003376_2_1_5"/>
    <dbReference type="OMA" id="YFPMLMT"/>
    <dbReference type="OrthoDB" id="464096at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008750">
        <property type="term" value="F:NAD(P)+ transhydrogenase (AB-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008143">
        <property type="entry name" value="Ala_DH/PNT_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007886">
        <property type="entry name" value="AlaDH/PNT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007698">
        <property type="entry name" value="AlaDH/PNT_NAD(H)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01262">
        <property type="entry name" value="AlaDh_PNT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05222">
        <property type="entry name" value="AlaDh_PNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01002">
        <property type="entry name" value="AlaDh_PNT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01003">
        <property type="entry name" value="AlaDh_PNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00837">
        <property type="entry name" value="ALADH_PNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AlaDh_PNT_N" evidence="4">
        <location>
            <begin position="4"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="AlaDh_PNT_C" evidence="3">
        <location>
            <begin position="145"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="376" mass="39480" checksum="9C449715E5343B8C" modified="2005-10-11" version="1">MKIAIAKEIDESEPRVAASPDTVKKFKALGADVAIEPGAGTKSGVPDSDFTAAGALVSPEAVKDADIIIKVRRPEASELARYKRGALVIAIMDPYGNEAALKKIADAGVSAFAMELMPRITRAQVMDVLSSQANLAGYRAVIEAAEAFGRAFPMMMTAAGTVPAAKVFVMGVGVAGLQAIATARRLGAAVTATDVRPATKEQVESLGAKFLAVEDEEFKNAQTAGGYAKEMSKEYQAKQAALTAEHIKKQDIIITTALIPGRPAPRLVTAEMVRSMKPGSVLVDLAVERGGNIEGVQAGKVVDLNGVKIIGFTNVAGRVAASASSLYARNLFSFIETLVDKTTKALAVNWDDELVKATALTRDGAVIHPNFQPKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STY9</accession>
    <name>Q3STY9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314291.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0990"/>
    <dbReference type="EnsemblBacteria" id="ABA04252">
        <property type="protein sequence ID" value="ABA04252"/>
        <property type="gene ID" value="Nwi_0990"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0990"/>
    <dbReference type="eggNOG" id="COG3108">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029918_1_0_5"/>
    <dbReference type="OMA" id="LFDILWE"/>
    <dbReference type="OrthoDB" id="285283at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010275">
        <property type="entry name" value="DUF882"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009045">
        <property type="entry name" value="Hedgehog_sig/DD-Pept_Zn-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37425">
        <property type="entry name" value="PTHR37425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05951">
        <property type="entry name" value="Peptidase_M15_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55166">
        <property type="entry name" value="SSF55166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="259"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="529" mass="57072" checksum="4C5DB2EF7A94F3A5" modified="2005-10-11" version="1">MLAGFARGLKSLSISRTGSRIGLSSLLLLAGAGSVHNAAALNETRTLSFHHTHSSENLTVTFKRNGRYDEGALKQLNHFLRDWRSQEQTTMDRHLFDILWEVYRDVDGRQPINIISAYRSPATNAMLRRRSKNSGVARFSQHTLGHAMDFYIPGVQLEKIRFAGLRLQRGGVGFYPKSGSPFVHLDTGHVRHWPRMTHDQLARVFPDGRTVHIPSDGNPLAGYQVAMADIEKRRRHAGEEGTASSKPSNFLLALFRGKSADEDDESDGAPAARPDQAPAPAPAATSADAVPLPRAKPGAFRLAAADVRPAPTPLSARQAEGSKPQTPADIINSRGFWDDIPPAPNQATQKQIAAAVSARRAVASAADRHTATGSISDPLQALAYAPEKDVRRARIFAATASIPPVRPAPLPARNPMAVDGVTTVVAKSAQGRKSTVSISRRLAPAASRDSTAWMRAMILSPSASRTMLATVMGEQDMTLMQAYFTKPDRTIAMAFSGDPQMGMVCDRFTGSAVARLTTTALAVQAASLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU59</accession>
    <name>Q3SU59_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0920</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04182">
        <property type="protein sequence ID" value="ABA04182"/>
        <property type="gene ID" value="Nwi_0920"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0920"/>
    <dbReference type="HOGENOM" id="CLU_153048_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229128" evidence="2">
        <location>
            <begin position="17"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="90" mass="9253" checksum="79AD4B03482855AD" modified="2005-10-11" version="1">MIISGLSLSFLSLSFAQDSMVIAQATQPHTSPVPSGKQDGPAESKPGGTRPTTPAPEPARPDADDARRANPEPALPPAPPEKVAPPIKGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU74</accession>
    <name>Q3SU74_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0905</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04167">
        <property type="protein sequence ID" value="ABA04167"/>
        <property type="gene ID" value="Nwi_0905"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0905"/>
    <dbReference type="HOGENOM" id="CLU_2155691_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="111" mass="12080" checksum="86CBAABDC4E7CCEF" modified="2005-10-11" version="1">MIRFAASKAEGVADGGFFVRDSDAGPRRSRTPRSDFRRVYVACGRGYIESPSDIESVLLRTNEAVSLRIKDVAPVSRSGRTSGAASLSSIFCAVEFRCSTSRLNRDGVRTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU87</accession>
    <name>Q3SU87_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase-like protein</fullName>
            <ecNumber evidence="2">3.1.2.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.2.6" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314195.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0890"/>
    <dbReference type="EnsemblBacteria" id="ABA04154">
        <property type="protein sequence ID" value="ABA04154"/>
        <property type="gene ID" value="Nwi_0890"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0890"/>
    <dbReference type="eggNOG" id="COG0491">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_6_1_5"/>
    <dbReference type="OMA" id="LYLCHDY"/>
    <dbReference type="OrthoDB" id="1581636at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004416">
        <property type="term" value="F:hydroxyacylglutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050313">
        <property type="term" value="F:sulfur dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07724">
        <property type="entry name" value="POD-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044528">
        <property type="entry name" value="POD-like_MBL-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="22"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="295" mass="33058" checksum="3919F72FDCB63C85" modified="2005-10-11" version="1">MSYPIDTQVKPKVIPFFDPETNTISYVVRDRSSLSCAVIDSVMDIDYAAGRISYKSADLIVAHIRRNGLQVDWLIETHAHADHLSAASYIQQRLGGKLGIGEHIVTVQETFGKVFNEGTEFKRDGSQFDRLFKDGDTYSIGGMTAFVMHTPGHTPACTTHVIGDAAFTGDTLFMPDAGTARADFPGGDARTLYRSIRKLLETLPPETRLFLCHDYGPDGRDIRWETTIREERMHNIHVRDGISEDSYVQMREARDETLSMPRLIMPSLQVNMRAGHLPEPDESGKTFLKVPINTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUM1</accession>
    <name>Q3SUM1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">3-hydroxyacyl-CoA dehydrogenase</fullName>
            <ecNumber evidence="6">1.1.1.35</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_0755</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 3-hydroxyacyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.35" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314074.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0755"/>
    <dbReference type="EnsemblBacteria" id="ABA04020">
        <property type="protein sequence ID" value="ABA04020"/>
        <property type="gene ID" value="Nwi_0755"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0755"/>
    <dbReference type="eggNOG" id="COG1250">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_2_0_5"/>
    <dbReference type="OMA" id="FYDYSES"/>
    <dbReference type="OrthoDB" id="862072at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003857">
        <property type="term" value="F:3-hydroxyacyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
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        <property type="term" value="P:cellular catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:organic substance catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="3-OHacyl-CoA_DH"/>
    </dbReference>
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        <property type="entry name" value="3-OHacyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006176">
        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006108">
        <property type="entry name" value="3HC_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00725">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02737">
        <property type="entry name" value="3HCDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000105">
        <property type="entry name" value="HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00067">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3HCDH_N" evidence="5">
        <location>
            <begin position="7"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="3HCDH" evidence="4">
        <location>
            <begin position="189"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="12"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="2">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000105-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000105-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA04020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="293" mass="31777" checksum="CEB5E84E09C81CAB" modified="2005-10-11" version="1">MAAMIRKVGVIGSGQMGNGIAHVTALAGFDVVLNDVSADQLNAAIATINANLSRQVAKKIITDGDREKALTRISQTETLDGLADCDLVIETAVEKEDVKRKIFHDLCAVLKPEAIVASNTSSISITRLAASTDRPEKFIGIHFMNPVPLMELVELIRGIATGDATFDAAKEFVAKLGKQIAVSEDFPAFIVNRILLPMINEAIYTLYEGVGNVEAIDAAMRLGAHHPMGPLELADFIGLDTCLSIMQVLHEGLADSKYRPCPLLVKYVEAGWLGRKSQRGFYDYRGEKPVPTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUR4</accession>
    <name>Q3SUR4_NITWN</name>
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        <recommendedName>
            <fullName evidence="1">FeS_assembly_P domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0711</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03977.1"/>
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        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ABA03977">
        <property type="protein sequence ID" value="ABA03977"/>
        <property type="gene ID" value="Nwi_0711"/>
    </dbReference>
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    <dbReference type="eggNOG" id="COG2151">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091588_1_2_5"/>
    <dbReference type="OMA" id="MKNFRDD"/>
    <dbReference type="OrthoDB" id="1668114at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014291">
        <property type="entry name" value="SUF_FeS_clus_asmbl-assoc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="SSF117916"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02945">
        <property type="entry name" value="SUF_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FeS_assembly_P" evidence="1">
        <location>
            <begin position="40"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="135" mass="14671" checksum="0629D175F32319E3" modified="2005-10-11" version="1">MTDEANVAGAESACLVEARTDSGTDHAANTPPDEMARMGEEIIAALKTVYDPEIPADLYELGLIYKIAIDDERKVNIEMTLTSPNCPSAAELPGQIERAVKGVSGVHDAKVAIVWEPTWDPSRMSDEARTVLNMW</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUX6</accession>
    <name>Q3SUX6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HAD-superfamily hydrolase subfamily IA, variant 3</fullName>
            <ecNumber evidence="1">3.1.3.18</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0649</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.3.18" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313975.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0649"/>
    <dbReference type="EnsemblBacteria" id="ABA03915">
        <property type="protein sequence ID" value="ABA03915"/>
        <property type="gene ID" value="Nwi_0649"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0649"/>
    <dbReference type="eggNOG" id="COG0637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_13_3_5"/>
    <dbReference type="OMA" id="KTWALAP"/>
    <dbReference type="OrthoDB" id="1167152at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008967">
        <property type="term" value="F:phosphoglycolate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="220" mass="23059" checksum="A1D284C7E35FFB8B" modified="2005-10-11" version="1">MPEADASPLHSGLFLDLDGTLADSVAALKNAYHSFLASFGASGDEAEFQRLNGPPLGEIIERLRMTHKLPGTPADLLQKYAAMVSQAHHSARPAAGAFELLAHARERGLKIAVVTSSPKLSAQGWLAVAGLADKIDDVVGGDEVTSGKPAAEPYIRALRRLNCSAALSHAVEDSRIGATAAVAAGLKTWALAPPKDRAGWPGQVVFIERLTDLLGRVPAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUY0</accession>
    <name>Q3SUY0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Acylneuraminate cytidylyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0645</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313971.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0645"/>
    <dbReference type="EnsemblBacteria" id="ABA03911">
        <property type="protein sequence ID" value="ABA03911"/>
        <property type="gene ID" value="Nwi_0645"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0645"/>
    <dbReference type="eggNOG" id="COG1861">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072501_0_0_5"/>
    <dbReference type="OMA" id="FIIQARM"/>
    <dbReference type="OrthoDB" id="1345588at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003329">
        <property type="entry name" value="Cytidylyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02348">
        <property type="entry name" value="CTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="241" mass="27250" checksum="ED98429FA368A4E0" modified="2005-10-11" version="1">MSNEAVIITQARMTSTRLPGKILMRAGERTLLETHLGRLAEVGVPVIVATTTNQSDNPVVELCAALDVPVFRGSENDVLERYQGAARAHEARHVIRVTSDCPLICPELIRAGLDIYLSLKSRSVYLSNAEKRTYPRGLDFEIFSRRSLDFAAENARLSSEREHVTSYIRTSMPDIHHESYTDQDDLSDWRITVDTDDDFKLVKLLIEHHRAAEMDYAALKALLKAHPRLMTINSHIQQKTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV77</accession>
    <name>Q3SV77_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">dTDP-4-dehydrorhamnose reductase</fullName>
            <ecNumber evidence="4 6">1.1.1.133</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_0547</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4-hexulose to yield dTDP-L-rhamnose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>dTDP-beta-L-rhamnose + NADP(+) = dTDP-4-dehydro-beta-L-rhamnose + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:21796"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57510"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:62830"/>
            <dbReference type="EC" id="1.1.1.133"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Binds 1 Mg(2+) ion per monomer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Carbohydrate biosynthesis; dTDP-L-rhamnose biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the dTDP-4-dehydrorhamnose reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.133" evidence="4 6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313875.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0547"/>
    <dbReference type="EnsemblBacteria" id="ABA03814">
        <property type="protein sequence ID" value="ABA03814"/>
        <property type="gene ID" value="Nwi_0547"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0547"/>
    <dbReference type="eggNOG" id="COG1091">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045518_1_0_5"/>
    <dbReference type="OMA" id="AGETTWH"/>
    <dbReference type="OrthoDB" id="1076083at2"/>
    <dbReference type="UniPathway" id="UPA00124"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008831">
        <property type="term" value="F:dTDP-4-dehydrorhamnose reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019305">
        <property type="term" value="P:dTDP-rhamnose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005913">
        <property type="entry name" value="dTDP_dehydrorham_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029903">
        <property type="entry name" value="RmlD-like-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10491">
        <property type="entry name" value="PTHR10491"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04321">
        <property type="entry name" value="RmlD_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01214">
        <property type="entry name" value="rmlD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="6 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RmlD_sub_bind" evidence="7">
        <location>
            <begin position="1"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA03814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="308" mass="32526" checksum="49F6A5EF59DD636C" modified="2005-10-11" version="1">MRILLTGSSGQVGGALRPLLKEGGMVIAPSRSAFDLSNPETLAGALDRFKPDLIINPAAYTAVDRAEDERELAFLVNAKAPAAIAEWSARHRVPLIHFSTDYVFDGSGDKPWREDSPTGPLSVYGASKLAGDLAIEAAGGPHLIARTSWVYAASGANFLRTIARLAGERKELRIVADQTGAPTTANTIADAVSRIVLSNASDLSALFARSGGVVNLACAGETSWHGFATAIVGGLKSRGAGLSVETIIPIATADFPTRARRPGNSRLDLSQLRERFGLTPPTWQDALSTELDRLVTGQDEQAVPAMRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVE5</accession>
    <name>Q3SVE5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0235 protein Nwi_0479</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0479</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0235 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313810.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0479"/>
    <dbReference type="EnsemblBacteria" id="ABA03746">
        <property type="protein sequence ID" value="ABA03746"/>
        <property type="gene ID" value="Nwi_0479"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0479"/>
    <dbReference type="eggNOG" id="COG1872">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130694_3_0_5"/>
    <dbReference type="OMA" id="EAWRYSA"/>
    <dbReference type="OrthoDB" id="1997369at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00634">
        <property type="entry name" value="UPF0235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003746">
        <property type="entry name" value="DUF167"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036591">
        <property type="entry name" value="YggU-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02594">
        <property type="entry name" value="DUF167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01152">
        <property type="entry name" value="DUF167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69786">
        <property type="entry name" value="SSF69786"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="106" mass="11165" checksum="F064E2E528B914F8" modified="2005-10-11" version="1">MEPWRYSSQGLAIALRVTPRGGRDAIDGIEMLADGRPVVKVRVRAVADGGEANRAVTAVLAKALGVRKIDVRILAGATSRLKQVAVEGDPVQLGNALRALTAVQPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVK0</accession>
    <name>Q3SVK0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0424</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313755.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0424"/>
    <dbReference type="EnsemblBacteria" id="ABA03691">
        <property type="protein sequence ID" value="ABA03691"/>
        <property type="gene ID" value="Nwi_0424"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0424"/>
    <dbReference type="HOGENOM" id="CLU_1600968_0_0_5"/>
    <dbReference type="OMA" id="HIYASEY"/>
    <dbReference type="OrthoDB" id="2118213at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="166" mass="18486" checksum="2E09EB313B2095CF" modified="2005-10-11" version="1">MTQILLKSNIRTAELGHLTAIDLELNAVILELVYLACLSFEHGSSAAGMYLSEYGGEIKTISYENPLDVLGDFKNISKKSADWVLSRTLFYHDNHRRRARADQEKASAEEKHQSIIEKKLGNLDKAHALRAKLIKDGADPDELNRLVGGLLFEQRAKLIPGQDEAG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVP7</accession>
    <name>RF1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptide chain release factor 1</fullName>
            <shortName evidence="1">RF-1</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">prfA</name>
        <name type="ordered locus">Nwi_0377</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="1">Methylated by PrmC. Methylation increases the termination efficiency of RF1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313708.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVP7"/>
    <dbReference type="SMR" id="Q3SVP7"/>
    <dbReference type="STRING" id="323098.Nwi_0377"/>
    <dbReference type="EnsemblBacteria" id="ABA03644">
        <property type="protein sequence ID" value="ABA03644"/>
        <property type="gene ID" value="Nwi_0377"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0377"/>
    <dbReference type="eggNOG" id="COG0216">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036856_0_1_5"/>
    <dbReference type="OMA" id="ISDHRVG"/>
    <dbReference type="OrthoDB" id="928964at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00093">
        <property type="entry name" value="Rel_fac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005139">
        <property type="entry name" value="PCRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004373">
        <property type="entry name" value="RF-1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03462">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00937">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="SSF75620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00019">
        <property type="entry name" value="prfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00745">
        <property type="entry name" value="RF_PROK_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0488">Methylation</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000263306" description="Peptide chain release factor 1">
        <location>
            <begin position="1"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="modified residue" description="N5-methylglutamine" evidence="1">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00093"/>
        </source>
    </evidence>
    <sequence length="358" mass="39437" checksum="480799D3B90222DA" modified="2005-10-11" version="1">MLPEAKLDLLLARHAALESELLSHVSADRYVQMTRELAELNPIVDAVKNWRAVTAEIAGIDAMIADPATDAEMRAMAEAERPALEARNDELMQKIRVALLPKDAMDERNVMLEVRAGTGGDEASLFAGDLFRMYERFAALQGWKVEVISASEGTMGGYKEIVAQVQGRGAYARMKFESGVHRVQRVPDTEAQGRVHTSAATVAVLPEAEEVDVDIRPDDLRIETMRAQGAGGQHVNKTESAIRITHIPTGIVVSMQDSRSQHKNRASAMNILRSRIYDAERQRINAARSADRKDQVGSGDRSERIRTYNFPQGRVTDHRINLTLYKLPQVIAGDALHELIDALTTEHQAAQLAAEGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVY0</accession>
    <name>Q3SVY0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Peptidase M17, leucyl aminopeptidase</fullName>
            <ecNumber evidence="7">3.4.11.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0294</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M17 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.1" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313627.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0294"/>
    <dbReference type="EnsemblBacteria" id="ABA03561">
        <property type="protein sequence ID" value="ABA03561"/>
        <property type="gene ID" value="Nwi_0294"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0294"/>
    <dbReference type="eggNOG" id="COG0260">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013734_2_1_5"/>
    <dbReference type="OMA" id="HFDIYGW"/>
    <dbReference type="OrthoDB" id="356206at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00433">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011356">
        <property type="entry name" value="Leucine_aapep/pepB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000819">
        <property type="entry name" value="Peptidase_M17_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963">
        <property type="entry name" value="PTHR11963"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00883">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00481">
        <property type="entry name" value="LAMNOPPTDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="SSF52949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00631">
        <property type="entry name" value="CYTOSOL_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CYTOSOL_AP" evidence="6">
        <location>
            <begin position="310"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="462" mass="49457" checksum="1B39230545AEDA77" modified="2005-10-11" version="1">MHPAFATTPAEKAVPIILASKAAWEADGDSLDDSARRFAEANGFQAKSGQYLALPTTNGDISRILFGLGEKPDQTRDPFLAGKLPGLLPPGAFRFADTATANDMHLATLAFALGSYRFDRYRKADAPKARLAPPDGVDIADISRMAEAASLARDLINTPANDMGPEDLATAARNLARRFDAEFNCIVGDDLMQRNFPLIHAVGMASPRAPRLIDLTWGDPAHPMVTLVGKGVCFDTGGVDLKTASGMQIMKKDMGGAANVLALALMVMDAKLKVRLRVLIPAVENAVAGNAFRPLDIFPSRKGLSVEIGNTDAEGRLVLADALALADEEAPELLIDVGTLTGAARVALGPDLPPFYTSDENLASDLARCGKTENDPLWRMPLWPAYDAWLDSKIADINNAPSGGFAGSIVCALFLQRFVEAAKTWLHVDIYGWTPSAKPARPEGGECQAARAIYRLLSERYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW03</accession>
    <name>Q3SW03_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Excisionase/Xis, DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0271</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0271"/>
    <dbReference type="EnsemblBacteria" id="ABA03538">
        <property type="protein sequence ID" value="ABA03538"/>
        <property type="gene ID" value="Nwi_0271"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0271"/>
    <dbReference type="eggNOG" id="COG3311">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1843019_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041657">
        <property type="entry name" value="HTH_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010093">
        <property type="entry name" value="SinI_DNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12728">
        <property type="entry name" value="HTH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01764">
        <property type="entry name" value="excise"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_17" evidence="1">
        <location>
            <begin position="80"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="139" mass="15718" checksum="4078D1A57404AF09" modified="2005-10-11" version="1">MWRACGKRDSRVEVAASRSQIDLWKSGDPSWPLATLRRLHKGSQGGRRLPALEFFSRIFADLPYNSPDSTCGERMADEILTIREVAELLKINEKTAYKLAAAGKLPGFKVGGSWRFERQEIANWIRRKVEEQQGGNQGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW33</accession>
    <name>Q3SW33_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0240</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313574.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0240"/>
    <dbReference type="EnsemblBacteria" id="ABA03508">
        <property type="protein sequence ID" value="ABA03508"/>
        <property type="gene ID" value="Nwi_0240"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0240"/>
    <dbReference type="eggNOG" id="ENOG5032YD6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110713_0_0_5"/>
    <dbReference type="OMA" id="DKGNGWA"/>
    <dbReference type="OrthoDB" id="1820785at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229276" evidence="2">
        <location>
            <begin position="29"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="180" mass="18979" checksum="CC9E1D710DDD0EE8" modified="2005-10-11" version="1">MDATPPLKLSRISAIAAIFCCLAAPALGQASEAGKARYTLMPMSGGFVRLDTLTGVVSHCEDKGSGWACYALPEEHAALDKEIGRLQAENEALKAQLAARESAGKTGKVVSGQEATGSGGSGSATPKADEPKSAEADRNSIEVPLPSDRDVGRLMSFVERAWRRLMELASRIEHDPSGRI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW60</accession>
    <name>ILVD_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dihydroxy-acid dehydratase</fullName>
            <shortName evidence="1">DAD</shortName>
            <ecNumber evidence="1">4.2.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ilvD</name>
        <name type="ordered locus">Nwi_0213</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Functions in the biosynthesis of branched-chain amino acids. Catalyzes the dehydration of (2R,3R)-2,3-dihydroxy-3-methylpentanoate (2,3-dihydroxy-3-methylvalerate) into 2-oxo-3-methylpentanoate (2-oxo-3-methylvalerate) and of (2R)-2,3-dihydroxy-3-methylbutanoate (2,3-dihydroxyisovalerate) into 2-oxo-3-methylbutanoate (2-oxoisovalerate), the penultimate precursor to L-isoleucine and L-valine, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:24809"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:24810"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate = (S)-3-methyl-2-oxopentanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:27694"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:27695"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="1">Binds 1 [2Fe-2S] cluster per subunit. This cluster acts as a Lewis acid cofactor.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IlvD/Edd family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.9" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313548.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SW60"/>
    <dbReference type="SMR" id="Q3SW60"/>
    <dbReference type="STRING" id="323098.Nwi_0213"/>
    <dbReference type="EnsemblBacteria" id="ABA03481">
        <property type="protein sequence ID" value="ABA03481"/>
        <property type="gene ID" value="Nwi_0213"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0213"/>
    <dbReference type="eggNOG" id="COG0129">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014271_4_3_5"/>
    <dbReference type="OMA" id="TQGRNMA"/>
    <dbReference type="OrthoDB" id="193579at2"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00057"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00061"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004160">
        <property type="term" value="F:dihydroxy-acid dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00012">
        <property type="entry name" value="IlvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042096">
        <property type="entry name" value="Dihydro-acid_dehy_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004404">
        <property type="entry name" value="DihydroxyA_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000581">
        <property type="entry name" value="DiOHA_6PGluconate_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020558">
        <property type="entry name" value="DiOHA_6PGluconate_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037237">
        <property type="entry name" value="IlvD/EDD_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00920">
        <property type="entry name" value="ILVD_EDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143975">
        <property type="entry name" value="SSF143975"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00110">
        <property type="entry name" value="ilvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00886">
        <property type="entry name" value="ILVD_EDD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00887">
        <property type="entry name" value="ILVD_EDD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0001">2Fe-2S</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000225399" description="Dihydroxy-acid dehydratase">
        <location>
            <begin position="1"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="517"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="1">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="491"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="1">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00012"/>
        </source>
    </evidence>
    <sequence length="614" mass="65467" checksum="B005A0938DDD4415" modified="2005-10-11" version="1">MPAYRSRTSTHGRNMAGARGLWRATGMKNEDFGKPIIAVVNSFTQFVPGHVHLKDLGQLVAREIEKAGGVAKEFHTIAVDDGIAMGHDGMLYSLPSREVIADSVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPSVFVSGGPMESGKVTLNGKVKSVDLIDAMVAAADDSVSDADVEAIERSACPTCGSCSGMFTANSMNCLTEALGLALPGNGSVLATHADRKGLFVEAGHLIVDMARRYYEQNDERVLPRSVASFKAFENAMTLDISMGGSTNTVLHLLAAAYEGEVPFTMADIDRLSRRVPVLCKVAPSVADVHLEDVHRAGGIMGILGELDRAGLIDASLPTVHSNSLREALERWDIKRTKSESVRTFYTAAPGGVRTEIAFSQDKRFEELDADRAKGCIRDAEHAFSKDGGLAVLYGNIARDGCIVKTAGVDDSILTFSGPARVFESQDAAVDAILGNRIKPGDVVLIRYEGPRGGPGMQEMLYPTSYLKSKGLGKQCALITDGRFSGGSSGLSIGHVSPEAAEGGAIGLVEEGDLIAFDIPNRKVHLDVSDAELERRRAAMEAKGDKAWKPAAPRTRRITMALKAYAAHTTSAALGAVRVVKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWB5</accession>
    <name>Q3SWB5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Bifunctional purine biosynthesis protein PurH</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="8">Phosphoribosylaminoimidazolecarboxamide formyltransferase</fullName>
                <ecNumber evidence="8">2.1.2.3</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="8">AICAR transformylase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="8">IMP cyclohydrolase</fullName>
                <ecNumber evidence="8">3.5.4.10</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="8">ATIC</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="8">IMP synthase</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="8">Inosinicase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="8" type="primary">purH</name>
        <name evidence="10" type="ordered locus">Nwi_0158</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(6S)-10-formyltetrahydrofolate + 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide = (6S)-5,6,7,8-tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:22192"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="EC" id="2.1.2.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>H2O + IMP = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:18445"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="EC" id="3.5.4.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.</text>
    </comment>
    <comment type="domain">
        <text evidence="8">The IMP cyclohydrolase activity resides in the N-terminal region.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the PurH family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.3" evidence="8"/>
    <dbReference type="EC" id="3.5.4.10" evidence="8"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313495.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0158"/>
    <dbReference type="EnsemblBacteria" id="ABA03426">
        <property type="protein sequence ID" value="ABA03426"/>
        <property type="gene ID" value="Nwi_0158"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0158"/>
    <dbReference type="eggNOG" id="COG0138">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016316_5_2_5"/>
    <dbReference type="OMA" id="WRVAKFV"/>
    <dbReference type="OrthoDB" id="1049662at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00133"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003937">
        <property type="term" value="F:IMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004643">
        <property type="term" value="F:phosphoribosylaminoimidazolecarboxamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00139">
        <property type="entry name" value="PurH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024051">
        <property type="entry name" value="AICAR_Tfase_dup_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002695">
        <property type="entry name" value="PurH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11692">
        <property type="entry name" value="PTHR11692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01808">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000414">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00798">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="SSF52335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00355">
        <property type="entry name" value="purH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="5 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MGS-like" evidence="9">
        <location>
            <begin position="2"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA03426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="530" mass="56464" checksum="ED1C34F918C58C06" modified="2005-10-11" version="1">MTDRPRRVTRALLSVSDKTGLTEFARALADLGVELVSTGGTAKEIAAAGLKVSDVSDLTGFPEMMDGRVKTLHPKVHGGLLAIRDNADHAKSMKDHGIAPIDLLVVNLYPFESTVDKGAACEECIENIDIGGPAMIRAAAKNHDDVAVVVEPQDYQAVLDELKANAGATTLSLRKRLAAKAYARTAAYDAAISNWFAVQLETDAPDYRAVGGRLAQRLRYGENPHQTAAFYRTPERRAGVATARQLQGKELSYNNINDTDAAYECVAEFDAARTAACVIIKHANPCGVAEGSSLTEAYRRALACDQTSAYGGIIAFNRTIDADAANAVAGIFTEVIIAPDATEEAIAVIGKRKNLRLLLAGGLPDPRARGLTAKTVAGGLLVQGRDNAVIDDMSLKVVTKRPPTEAEMRDLRFAFRVAKHVKSNTIVYARDLATVGIGAGQMSRVDSARIAARKAEDAARDLKLAEPLTKGSVVASDAFFPFADGMLACIKAGATAVIQPGGSMRDEEVIKAADEHGIAMVFTGVRHFRH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWG9</accession>
    <name>COAE_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dephospho-CoA kinase</fullName>
            <ecNumber evidence="1">2.7.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Dephosphocoenzyme A kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">coaE</name>
        <name type="ordered locus">Nwi_0104</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3'-dephospho-CoA + ATP = ADP + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18245"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 5/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CoaE family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.24" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313441.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWG9"/>
    <dbReference type="SMR" id="Q3SWG9"/>
    <dbReference type="STRING" id="323098.Nwi_0104"/>
    <dbReference type="EnsemblBacteria" id="ABA03372">
        <property type="protein sequence ID" value="ABA03372"/>
        <property type="gene ID" value="Nwi_0104"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0104"/>
    <dbReference type="eggNOG" id="COG0237">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057180_3_0_5"/>
    <dbReference type="OMA" id="QMDIEQK"/>
    <dbReference type="OrthoDB" id="1515383at2"/>
    <dbReference type="UniPathway" id="UPA00241">
        <property type="reaction ID" value="UER00356"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004140">
        <property type="term" value="F:dephospho-CoA kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00376">
        <property type="entry name" value="Dephospho_CoA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001977">
        <property type="entry name" value="Depp_CoAkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01121">
        <property type="entry name" value="CoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00152">
        <property type="entry name" value="TIGR00152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51219">
        <property type="entry name" value="DPCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000243308" description="Dephospho-CoA kinase">
        <location>
            <begin position="1"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="DPCK" evidence="1">
        <location>
            <begin position="3"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="11"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00376"/>
        </source>
    </evidence>
    <sequence length="199" mass="21613" checksum="02BF71E19FFA9B52" modified="2005-10-11" version="1">MLVIGLTGSIGMGKSTTARLFSEAGVPVYDADATVHAIYEGEAAFLVEAAFPGTTVNGKVDREKLSAKVVHDAAAMKRLEQIVHPLLYDYRQAFLQQAERSGAAVAVIDVPLLFETGGERSVDAVVVVTTTPEIQRERILARGNMTSEKLKAIQARQLPDAEKRKRADFVVDTSHGVDAVRTQIREILAAAAKMPRRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWI0</accession>
    <name>Q3SWI0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNA polymerase III, delta subunit</fullName>
            <ecNumber evidence="3">2.7.7.7</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0093</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.7" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313430.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0093"/>
    <dbReference type="EnsemblBacteria" id="ABA03361">
        <property type="protein sequence ID" value="ABA03361"/>
        <property type="gene ID" value="Nwi_0093"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0093"/>
    <dbReference type="eggNOG" id="COG1466">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068860_1_0_5"/>
    <dbReference type="OMA" id="WRGLHFR"/>
    <dbReference type="OrthoDB" id="1199768at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009360">
        <property type="term" value="C:DNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010372">
        <property type="entry name" value="DNA_pol3_delta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005790">
        <property type="entry name" value="DNA_polIII_delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06144">
        <property type="entry name" value="DNA_pol3_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="SSF48019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01128">
        <property type="entry name" value="holA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA_pol3_delta" evidence="2">
        <location>
            <begin position="24"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="342" mass="36543" checksum="173ECBDBB5BD1A22" modified="2005-10-11" version="1">MVALRGKDIDGFLARPDPARPIILLYGPDAGLVRERANALISSAVEDPNDPFSLVRLDGDDLAAEPSRLVDEAMTVPLLGGRRAIRIKAGSRSFASGVDTLANSTIQDCRIVIEAGELRPESPLRKACERAKNAVAIACYPDTERDLARLIDEELRASDLKIAPDARAALMSLLGGDRQASRNELRKIALYAHGQGEITLLDVMAVVSDASDFKLDPVIDAAFAGRPAAVEAEFAKAMVAGTYPGLVISAAQRFVAALHKAALLMEEGASASSATERGFPRLHFSRKASVETALSHFSAPRLVQVMDQLARAALETRKQAALASAITQRALMSIAVNARRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWJ7</accession>
    <name>Q3SWJ7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nitrogen regulatory protein P-II</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0076</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">In nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP. P-II-UMP allows the deadenylation of glutamine synthetase (GS), thus activating the enzyme. Conversely, in nitrogen excess P-II is deuridylated and promotes the adenylation of GS. P-II indirectly controls the transcription of the GS gene (glnA). P-II prevents NR-II-catalyzed conversion of NR-I to NR-I-phosphate, the transcriptional activator of glnA. When P-II is uridylylated to P-II-UMP, these events are reversed.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotrimer.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313413.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0076"/>
    <dbReference type="EnsemblBacteria" id="ABA03344">
        <property type="protein sequence ID" value="ABA03344"/>
        <property type="gene ID" value="Nwi_0076"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0076"/>
    <dbReference type="eggNOG" id="COG0347">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082268_0_0_5"/>
    <dbReference type="OMA" id="CIRIRTE"/>
    <dbReference type="OrthoDB" id="2021322at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002187">
        <property type="entry name" value="N-reg_PII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002332">
        <property type="entry name" value="N-reg_PII_urydylation_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30115">
        <property type="entry name" value="PTHR30115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00543">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039144">
        <property type="entry name" value="GlnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00340">
        <property type="entry name" value="PIIGLNB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00938">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51343">
        <property type="entry name" value="PII_GLNB_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00496">
        <property type="entry name" value="PII_GLNB_UMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="O-UMP-tyrosine" evidence="5">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR039144-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602187-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="112" mass="12114" checksum="ADEE79E35F4FE92C" modified="2005-10-11" version="1">MKLVVAIIKPFKLDEVREALTALGVHGMTVTEVKGYGRQKGHTEIYRGAEYVVNFLPKLRIEVAVASELADKAVEAIAAAARTGQIGDGKLFVLPIDHALRIRTGETDSDAL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWK8</accession>
    <name>SYFB_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phenylalanine--tRNA ligase beta subunit</fullName>
            <ecNumber evidence="1">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Phenylalanyl-tRNA synthetase beta subunit</fullName>
            <shortName evidence="1">PheRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pheT</name>
        <name type="ordered locus">Nwi_0065</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + H(+) + L-phenylalanyl-tRNA(Phe)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium ions per tetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313404.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWK8"/>
    <dbReference type="SMR" id="Q3SWK8"/>
    <dbReference type="STRING" id="323098.Nwi_0065"/>
    <dbReference type="EnsemblBacteria" id="ABA03333">
        <property type="protein sequence ID" value="ABA03333"/>
        <property type="gene ID" value="Nwi_0065"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0065"/>
    <dbReference type="eggNOG" id="COG0072">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0073">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016891_0_0_5"/>
    <dbReference type="OMA" id="ISYNWLK"/>
    <dbReference type="OrthoDB" id="53568at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00769">
        <property type="entry name" value="PheRS_beta_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02796">
        <property type="entry name" value="tRNA_bind_bactPheRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00283">
        <property type="entry name" value="Phe_tRNA_synth_beta1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005146">
        <property type="entry name" value="B3/B4_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005121">
        <property type="entry name" value="Fdx_antiC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036690">
        <property type="entry name" value="Fdx_antiC-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045060">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004532">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020825">
        <property type="entry name" value="Phe-tRNA_synthase-like_B3/B4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041616">
        <property type="entry name" value="PheRS_beta_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033714">
        <property type="entry name" value="tRNA_bind_bactPheRS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005147">
        <property type="entry name" value="tRNA_synthase_B5-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947">
        <property type="entry name" value="PTHR10947"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03483">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03484">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03147">
        <property type="entry name" value="FDX-ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17759">
        <property type="entry name" value="tRNA_synthFbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00873">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00874">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00896">
        <property type="entry name" value="FDX-ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54991">
        <property type="entry name" value="SSF54991"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00472">
        <property type="entry name" value="pheT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51483">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51447">
        <property type="entry name" value="FDX_ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000232068" description="Phenylalanine--tRNA ligase beta subunit">
        <location>
            <begin position="1"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA-binding" evidence="1">
        <location>
            <begin position="39"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="B5" evidence="1">
        <location>
            <begin position="405"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="FDX-ACB" evidence="1">
        <location>
            <begin position="718"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="458"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="464"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="467"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="468"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00283"/>
        </source>
    </evidence>
    <sequence length="812" mass="87077" checksum="72025ED7133ECA70" modified="2005-10-11" version="1">MKFTLSWLKDHLDTDEPLEKLADKLTMIGLEVEHIADKSKTFAPFTIARVVSAVQHPNADRLRVCMVDTGETSDKGDPSYIQVVCGAPNAREGLISVFAPPGTHIPGKNITLGVGTIRGVESRGMLCSAAELEISDDHDGIIELPADAPVGAGYAEWAGLGDPVIEINLTPNRQDCTGVHGIARDLSAADMGRFKDPTIKPVTGEFPCPVKVTVEDASLCPGFALRMVRGVKNGPSPEWLQKRLTSIGLRPINALVDITNFMTYDRARPLHVFDAKKVHGDLTIRRARDGETLKALDGRIYTLDPNVCVIADDKGVESLAGIMGGEETGCDETTTDVLIESALWSEINIAQTGRRLGINSDARYRFERGVDPAFMVPGLEMATRLVMELCGGSPSENVVAGQALPEDRVIDFPLSEIKRLAAIDVPLVEVRRILGHLGFMVAGSGPVVKVAIPTWRTDVQGKADLVEEIVRIVGVDKVPMTPFDRGEAPRKPVLTSIQSRTRKARRALAARGMVEAVNWSFISRTQAELFGGGSSELALANPIASDLSDMRPSLLPGLVAIAQANADRGFADVALFEVGQIFRGDGPQDQFMAAAGLRRGIASSAGLGRHWSGSATANALDAKADAFAVLAAAGAPAAALQIATSHESKNFPAWLHPGRSAAIQIGPHNVLGYFGELHPRVLDELKAEGPLLGFEVILDRIPEAKQKPTRAKPVLELPAFQPVSRDFAFIVDRTVKAADIVRAAQNVDRKLVSGVTVFDIYEGKGIDADKKSVAIAVRLQPRDRTFTDQEIEAVAGKIVAEVAKRTGGSLRG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-05-05" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMT7</accession>
    <name>MIAB_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase</fullName>
            <ecNumber evidence="1">2.8.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA-i(6)A37 methylthiotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">miaB</name>
        <name type="ordered locus">Nwi_0014</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i(6)A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[sulfur carrier]-SH + AH2 + N(6)-dimethylallyladenosine(37) in tRNA + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-dimethylallyladenosine(37) in tRNA + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H(+) + L-methionine + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:37067"/>
            <dbReference type="Rhea" id="RHEA-COMP:10375"/>
            <dbReference type="Rhea" id="RHEA-COMP:10376"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:74415"/>
            <dbReference type="ChEBI" id="CHEBI:74417"/>
            <dbReference type="EC" id="2.8.4.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methylthiotransferase family. MiaB subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.3" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SMT7"/>
    <dbReference type="SMR" id="Q3SMT7"/>
    <dbReference type="STRING" id="323098.Nwi_0014"/>
    <dbReference type="EnsemblBacteria" id="ABA03282">
        <property type="protein sequence ID" value="ABA03282"/>
        <property type="gene ID" value="Nwi_0014"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0014"/>
    <dbReference type="eggNOG" id="COG0621">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018697_2_0_5"/>
    <dbReference type="OMA" id="CEHFHIP"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035596">
        <property type="term" value="F:methylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
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        <property type="entry name" value="Methylthiotransferase"/>
    </dbReference>
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        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013848">
        <property type="entry name" value="Methylthiotransferase_N"/>
    </dbReference>
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        <property type="entry name" value="Methylthiotransferase_N_sf"/>
    </dbReference>
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        <property type="entry name" value="MiaB_methiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF04055">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00919">
        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00273">
        <property type="entry name" value="(dimethylallyl)adenosine_tRNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01061">
        <property type="entry name" value="methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00089">
        <property type="entry name" value="TIGR00089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000374409" description="tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase">
        <location>
            <begin position="1"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="MTTase N-terminal" evidence="1">
        <location>
            <begin position="9"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="156"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="1">
        <location>
            <begin position="391"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="454"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="477" mass="52190" checksum="9184011A283F6528" modified="2005-10-11" version="1">MPESRMPPRKLHIKSYGCQMNVYDAQRMADTLAVEGYVETTSAEDADLVILNTCHIREKASEKVYSELGRLRAAKDQAARDGRGMNIVVAGCVAQAEGEEIIRRAPMVDVVVGPQSYHHLPQLLARAKTCGRALETEFPVADKFGFLPQPSRQAIRSRGISAFVTVQEGCDKFCTFCVVPYTRGAEASRPVAKVVEDVRRLADNGVREITLIGQNVNAYHGEGPDGRPWPFGKLLYRLAGIPGIVRLRYSTSHPRDVEDTLIDAHRDLDALMPFVHLPVQSGSDRILAAMNRRHTADDYRRVIDRFRQARPDIAFSSDFIVGFPGETEEDFAATLALVGQIGYAAAYSFKYSPRPGTPAADMQETVSTADMDQRLVQLQNLIDSQQSAFNRTALGRTIDVLFDRAGRKPGQIVGRTAYLQPAHVEASDAIIGQVLPVTVASLERYSLFGTLASKPTSGEPSNHAATGGAQFQTTAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMU5</accession>
    <name>Q3SMU5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase, IS4 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0006</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0006"/>
    <dbReference type="EnsemblBacteria" id="ABA03274">
        <property type="protein sequence ID" value="ABA03274"/>
        <property type="gene ID" value="Nwi_0006"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0006"/>
    <dbReference type="eggNOG" id="COG3385">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055261_9_1_5"/>
    <dbReference type="OMA" id="FIAFINF"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE_Tnp_1" evidence="1">
        <location>
            <begin position="4"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="122" mass="13576" checksum="B051EB5338D483F8" modified="2005-10-11" version="1">MLADALGRPLRFIVTAGQVGDVTQAPALLEAQAGDAVLADKAYDSNALRALIAGMGAEAVIPSNRTRKIIIPHDAGLYKHRNRIERCFNRLKHFRRFATRYDRRTVHFTGFVHLAAALIWLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMX0</accession>
    <name>Q3SMX0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_3124</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + O-phospho-L-tyrosyl-[protein] = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:10137"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:82620"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316279.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3124"/>
    <dbReference type="EnsemblBacteria" id="ABA06371">
        <property type="protein sequence ID" value="ABA06371"/>
        <property type="gene ID" value="Nwi_3124"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3124"/>
    <dbReference type="eggNOG" id="COG0394">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071415_3_0_5"/>
    <dbReference type="OMA" id="CRSILCE"/>
    <dbReference type="OrthoDB" id="1800563at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023485">
        <property type="entry name" value="Ptyr_pPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036196">
        <property type="entry name" value="Ptyr_pPase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01451">
        <property type="entry name" value="LMWPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00226">
        <property type="entry name" value="LMWPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52788">
        <property type="entry name" value="SSF52788"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LMWPc" evidence="4">
        <location>
            <begin position="11"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA06371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="180" mass="19282" checksum="CE7B6ABA251F37A4" modified="2005-10-11" version="1">MSDPDQPNRIFNVLFLCTGNSARSILAESIAAKDGAGRFRSFSAGSQPKGEVNPFALKVLKNFDYPADGFRSKSWEEFAGPDAPVMDFVFTVCDNAAGETCPVWPGQPITAHWGIEDPAAVEGSDFQKEAAFVAALRYLKNRFSIFAALPVASLDRASLRAKLVEIGQSDGASSSRNSAA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN20</accession>
    <name>ENGB_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable GTP-binding protein EngB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">engB</name>
        <name type="ordered locus">Nwi_3073</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Necessary for normal cell division and for the maintenance of normal septation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. EngB GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316237.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SN20"/>
    <dbReference type="SMR" id="Q3SN20"/>
    <dbReference type="STRING" id="323098.Nwi_3073"/>
    <dbReference type="EnsemblBacteria" id="ABA06321">
        <property type="protein sequence ID" value="ABA06321"/>
        <property type="gene ID" value="Nwi_3073"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3073"/>
    <dbReference type="eggNOG" id="COG0218">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033732_2_0_5"/>
    <dbReference type="OMA" id="LMMDIRH"/>
    <dbReference type="OrthoDB" id="1746051at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01876">
        <property type="entry name" value="YihA_EngB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00321">
        <property type="entry name" value="GTPase_EngB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030393">
        <property type="entry name" value="G_ENGB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019987">
        <property type="entry name" value="GTP-bd_ribosome_bio_YsxC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03598">
        <property type="entry name" value="GTPase_YsxC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51706">
        <property type="entry name" value="G_ENGB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0717">Septation</keyword>
    <feature type="chain" id="PRO_0000266905" description="Probable GTP-binding protein EngB">
        <location>
            <begin position="1"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="EngB-type G" evidence="1">
        <location>
            <begin position="37"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="45"/>
            <end position="52"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="72"/>
            <end position="76"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="92"/>
            <end position="95"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="159"/>
            <end position="162"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="193"/>
            <end position="195"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00321"/>
        </source>
    </evidence>
    <sequence length="217" mass="23585" checksum="7595FF1598B040B1" modified="2005-10-11" version="1">MTTGLDAELIEQGRKLFAGEWQFIWASPSIETLPPMSGLEVAFAGRSNVGKSSLINALTGRSALARTSRTPGRTQELIFFEGPKGVDVRLVDMPGYGFASAPKARIASWTSLIHQFLLGRATLARVYVLIDARHGIKDIDKTVLGALDRSAVSYQVVLTKADEVKVTEMAERIDITRTQLASHPAAFPDVLVTSSRTGNGIPELRAAMARLIGDHRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN26</accession>
    <name>Q3SN26_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">5'-methylthioadenosine nucleosidase / S-adenosylhomocysteine nucleosidase</fullName>
            <ecNumber evidence="2">3.2.2.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_3067</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.2.2.16" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316231.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3067"/>
    <dbReference type="EnsemblBacteria" id="ABA06315">
        <property type="protein sequence ID" value="ABA06315"/>
        <property type="gene ID" value="Nwi_3067"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3067"/>
    <dbReference type="eggNOG" id="COG0775">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107471_0_0_5"/>
    <dbReference type="OMA" id="WTEYLHI"/>
    <dbReference type="OrthoDB" id="1860206at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008930">
        <property type="term" value="F:methylthioadenosine nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009116">
        <property type="term" value="P:nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="SSF53167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNP_UDP_1" evidence="1">
        <location>
            <begin position="23"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="184" mass="20121" checksum="E5492E11F3F56624" modified="2005-10-11" version="1">MSRILVLTAIEDELGRARLPDGVRVIYTGVGKINAASAATLALLTLHPDLVINYGTAGKINETLRGLVEVSTVVQRDMMAMPLAPRGRTPFAPELDLLTSGQSGVVCGTGDSFVTSSDPWLIENNVDIVDMELFAVAQVCRRHGLPWRAFKFITDDANDLAHQDWTANVKDGEGLFWDTLDRIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN36</accession>
    <name>Q3SN36_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Fec I like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_3056</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316221.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06305">
        <property type="protein sequence ID" value="ABA06305"/>
        <property type="gene ID" value="Nwi_3056"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3056"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_3_0_5"/>
    <dbReference type="OMA" id="HGRLCRF"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="6">
        <location>
            <begin position="41"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4" evidence="7">
        <location>
            <begin position="142"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA06305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="203" mass="22749" checksum="887F17B5AB6B8C55" modified="2005-10-11" version="1">MQNVIAISAAACQGIVAAQTTTDEMLLKRIAHGDCSAMHTLYLRHNVRVYRFVLRSLRDATAAEDIVSQVFLDVWRTADQFEGRSRVSTWLLSIARFKTLTAMRQRRHEDIDQENVREIADGADTPETSLDRSNVSAILRACIAKLSPAHREIIHLVYYHEKTVEEAGEIIGIPPSTVKTRMFYARKQLATLLKGAGLETLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN64</accession>
    <name>Q3SN64_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_3027</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316195.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3027"/>
    <dbReference type="EnsemblBacteria" id="ABA06277">
        <property type="protein sequence ID" value="ABA06277"/>
        <property type="gene ID" value="Nwi_3027"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3027"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_3_5"/>
    <dbReference type="OMA" id="QAFFYNA"/>
    <dbReference type="OrthoDB" id="1121832at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="122"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="278"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="313"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="343"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="373"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="404"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="438"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="29"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA06277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="481" mass="52046" checksum="63736534C64532E6" modified="2005-10-11" version="1">MQERNDRIVETDIPARLDSLRWSPFHTRVVTALGITWILDGLEVTLAGALSGALKDSPTLQFSNLDIGIAGSAYLIGAVLGALGFGWLTDRIGRKKLFFATLTLYLVATAATALSWDLASFALFRFFTGAGIGGEYTAINSTIQELVPARYRGWTDLTINGSFWIGAALGAAGSIVLLDPQVIDPELGWRLAFLTGAVLGLVVFVMRLWIPESPRWLMVHGRPEEAARIVAGIERRAVRDPDEPAGGVWPVIELRMRSHTPLREVARTLFKVYRKRSLVGLALMTAQAFFYNAIFFTYALVLSTFFNIPADQIGWYLLPFAASNFLGPLLLGRLFDTVGRRAMIAFTYGISGILLAITGYLFAAGVLTAQTQMIAWMVIFFFASPAASAAYLTVSETFPLEVRALAIAVFYCVGTAIGGVAGPALFGALIDSGSRDSVFIGYCFGSALMVAAALIGWRFGIAAERRPLESVASPLAAVESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNB5</accession>
    <name>Q3SNB5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">FeoA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2976</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FeoA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2976"/>
    <dbReference type="EnsemblBacteria" id="ABA06226">
        <property type="protein sequence ID" value="ABA06226"/>
        <property type="gene ID" value="Nwi_2976"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2976"/>
    <dbReference type="eggNOG" id="COG1918">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2684168_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007167">
        <property type="entry name" value="Fe-transptr_FeoA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038157">
        <property type="entry name" value="FeoA_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008988">
        <property type="entry name" value="Transcriptional_repressor_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04023">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00899">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50037">
        <property type="entry name" value="SSF50037"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FeoA" evidence="3">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA06226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="74" mass="7903" checksum="881EE1EDD5B959AE" modified="2005-10-11" version="1">MVGGDHLGVSANEIERRLLEFGIVEGASVQILHEGPVGRDPIAVRVNGTTIALRRREAMAIMVGDHDQAAKTVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNB7</accession>
    <name>Q3SNB7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2974</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316146.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2974"/>
    <dbReference type="EnsemblBacteria" id="ABA06224">
        <property type="protein sequence ID" value="ABA06224"/>
        <property type="gene ID" value="Nwi_2974"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2974"/>
    <dbReference type="HOGENOM" id="CLU_2845395_0_0_5"/>
    <dbReference type="OMA" id="DHATDKA"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="65" mass="7290" checksum="EE008DF42B818338" modified="2005-10-11" version="1">MASNGGETDDRRRKTKKELDEELDRGLEESFPGSDPLSVTQPAPAKPPEENHPKEKPTKEESTRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNC1</accession>
    <name>Q3SNC1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2970</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316142.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2970"/>
    <dbReference type="EnsemblBacteria" id="ABA06220">
        <property type="protein sequence ID" value="ABA06220"/>
        <property type="gene ID" value="Nwi_2970"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2970"/>
    <dbReference type="eggNOG" id="COG3637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1049219_0_0_5"/>
    <dbReference type="OMA" id="EANYMHG"/>
    <dbReference type="OrthoDB" id="2018542at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004228989" evidence="1">
        <location>
            <begin position="20"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="276" mass="29874" checksum="F72DE41FC1D328CE" modified="2005-10-11" version="1">MRRLLLAAVICGAAHGAYAADMPDDFPFLRGSQPPSLSTVRTVWQGFYIGGQASYGSADMDFKNSGKDLLERLLNHVDVESQFNLSSWPLGGTSSNRNSGFGGFLGYNLQMEDVVFGVEANYTHSKFLGASSGSQGRSFSYPADYRTSAFVQSNASMQIMDFGSLRVRAGYSLGSFLPYAFGGVAMGQANIRRNASYNLHYQYVGTQIPPLPNLAGGGSLSDNANAHFIYGYAAGLGIDMMIVDGLFLRAEWEFLRFTAPVDTIVNTVRAGAGYRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SND2</accession>
    <name>Q3SND2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2959</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316131.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2959"/>
    <dbReference type="EnsemblBacteria" id="ABA06209">
        <property type="protein sequence ID" value="ABA06209"/>
        <property type="gene ID" value="Nwi_2959"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2959"/>
    <dbReference type="eggNOG" id="ENOG5033AJG">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_184297_0_0_5"/>
    <dbReference type="OMA" id="MPQREAC"/>
    <dbReference type="OrthoDB" id="2071481at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="87" mass="10141" checksum="A2747CB875FA38C4" modified="2005-10-11" version="1">MPDFDALRDYLKQQTQAEFVLSFAQIEQIIDDDLPRAAQRASWWDTARAPEQRMPQREACIAAGYVATRLGEGVRFRRADAKRPRFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNI2</accession>
    <name>Q3SNI2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2909</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06159">
        <property type="protein sequence ID" value="ABA06159"/>
        <property type="gene ID" value="Nwi_2909"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2909"/>
    <dbReference type="eggNOG" id="ENOG5030XY6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2058904_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="127" mass="14137" checksum="64A213331EC0359C" modified="2005-10-11" version="1">MMPKPYAWRTEGRPAGSRGMRILTVAFALGSALALCCSGAFIAPVHAKPRRVIIVTPPTPQPYWDSYIVPRYQYRPEDDRVDPCGGPVVPVIRYGAQEATIPFWLANDLGLGLVHGRDWPCRGRGYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNI4</accession>
    <name>Q3SNI4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, ArsR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2907</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2907"/>
    <dbReference type="EnsemblBacteria" id="ABA06157">
        <property type="protein sequence ID" value="ABA06157"/>
        <property type="gene ID" value="Nwi_2907"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2907"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097806_7_4_5"/>
    <dbReference type="OMA" id="YHVEHLR"/>
    <dbReference type="OrthoDB" id="1772343at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="28"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="122" mass="13674" checksum="4F1CF0B6E22A2638" modified="2005-10-11" version="1">MPGGLTNTIRYSIVHWNIGFSGEMVSDPQWHQLELAAEMLKALADPHRLGILLRLSKRELSVGELAEIEQEKVTTMSARLKVLLTAHLVKRRRKGQSVLYSLADTHVLNLVDNAIEHACEGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNK2</accession>
    <name>Q3SNK2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 12">Epoxyqueuosine reductase QueH</fullName>
            <ecNumber evidence="5 12">1.17.99.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 12">Queuosine biosynthesis protein QueH</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">queH</name>
        <name evidence="13" type="ordered locus">Nwi_2889</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 12">Catalyzes the conversion of epoxyqueuosine (oQ) to queuosine (Q), which is a hypermodified base found in the wobble positions of tRNA(Asp), tRNA(Asn), tRNA(His) and tRNA(Tyr).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>AH2 + epoxyqueuosine(34) in tRNA = A + H2O + queuosine(34) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:32159"/>
            <dbReference type="Rhea" id="RHEA-COMP:10345"/>
            <dbReference type="Rhea" id="RHEA-COMP:10346"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:82831"/>
            <dbReference type="ChEBI" id="CHEBI:82834"/>
            <dbReference type="EC" id="1.17.99.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">tRNA modification; tRNA-queuosine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the QueH family.</text>
    </comment>
    <dbReference type="EC" id="1.17.99.6" evidence="5 12"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2889"/>
    <dbReference type="EnsemblBacteria" id="ABA06139">
        <property type="protein sequence ID" value="ABA06139"/>
        <property type="gene ID" value="Nwi_2889"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2889"/>
    <dbReference type="eggNOG" id="COG1636">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088177_0_0_5"/>
    <dbReference type="OMA" id="PNIHPYT"/>
    <dbReference type="UniPathway" id="UPA00392"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052693">
        <property type="term" value="F:epoxyqueuosine reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008616">
        <property type="term" value="P:queuosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02089">
        <property type="entry name" value="QueH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003828">
        <property type="entry name" value="QueH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36701">
        <property type="entry name" value="PTHR36701"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02677">
        <property type="entry name" value="QueH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 12" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-0671">Queuosine biosynthesis</keyword>
    <keyword evidence="12" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0819">tRNA processing</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="12">
        <location>
            <begin position="235"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA06139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="272" mass="31421" checksum="C29B85DAF0586C14" modified="2005-10-11" version="1">MLAIHKRGRIQPGRNFHQSIDRPLTLRLFRPLSRRTGCGVVPAPQPLSLTVTEFVRPNLTPPNGQKKVLLHSCCAPCSGEVMEAMLASGIDYTIFFYNPNIHPLKEYLLRKDENVRFAEKHGVPFVDADYDKDNWFARARGMEWEPERGARCTMCFDMRFERTALYAHEHGFPVMTSCLGISRWKNMAQINDCGHRAAGRYPGVSYWDFNWRKGGGAQRMIEISKREQFYQQEYCGCVYSLRDTNQHRRNHGRAPIVIGTQFYRQGEEPDAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNS1</accession>
    <name>Q3SNS1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA-dihydrouridine(20/20a) synthase</fullName>
            <ecNumber evidence="6">1.3.1.91</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">U20-specific dihydrouridine synthase</fullName>
            <shortName evidence="6">U20-specific Dus</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">tRNA-dihydrouridine synthase A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">dusA</name>
        <name evidence="10" type="ordered locus">Nwi_2817</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>5,6-dihydrouridine(20) in tRNA + NAD(+) = H(+) + NADH + uridine(20) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:53340"/>
            <dbReference type="Rhea" id="RHEA-COMP:13533"/>
            <dbReference type="Rhea" id="RHEA-COMP:13534"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.91"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>5,6-dihydrouridine(20) in tRNA + NADP(+) = H(+) + NADPH + uridine(20) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:53336"/>
            <dbReference type="Rhea" id="RHEA-COMP:13533"/>
            <dbReference type="Rhea" id="RHEA-COMP:13534"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.91"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>5,6-dihydrouridine(20a) in tRNA + NAD(+) = H(+) + NADH + uridine(20a) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:53348"/>
            <dbReference type="Rhea" id="RHEA-COMP:13535"/>
            <dbReference type="Rhea" id="RHEA-COMP:13536"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>5,6-dihydrouridine(20a) in tRNA + NADP(+) = H(+) + NADPH + uridine(20a) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:53344"/>
            <dbReference type="Rhea" id="RHEA-COMP:13535"/>
            <dbReference type="Rhea" id="RHEA-COMP:13536"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6 7">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the Dus family. DusA subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the dus family.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.91" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316015.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2817"/>
    <dbReference type="EnsemblBacteria" id="ABA06070">
        <property type="protein sequence ID" value="ABA06070"/>
        <property type="gene ID" value="Nwi_2817"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2817"/>
    <dbReference type="eggNOG" id="COG0042">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013299_2_1_5"/>
    <dbReference type="OMA" id="LIYPYVD"/>
    <dbReference type="OrthoDB" id="1320183at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102264">
        <property type="term" value="F:tRNA-dihydrouridine20 synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02041">
        <property type="entry name" value="DusA_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001269">
        <property type="entry name" value="DUS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004653">
        <property type="entry name" value="DusA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42907">
        <property type="entry name" value="PTHR42907"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006621">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00742">
        <property type="entry name" value="yjbN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6 7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 6 7" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="5 6 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="6" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Dus" evidence="9">
        <location>
            <begin position="11"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6 8">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="13"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="207"/>
            <end position="209"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="229"/>
            <end position="230"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="site" description="Interacts with tRNA" evidence="6">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <feature type="site" description="Interacts with tRNA; defines subfamily-specific binding signature" evidence="6">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <feature type="site" description="Interacts with tRNA" evidence="6">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <feature type="site" description="Interacts with tRNA; defines subfamily-specific binding signature" evidence="6">
        <location>
            <position position="295"/>
        </location>
    </feature>
    <feature type="site" description="Interacts with tRNA; defines subfamily-specific binding signature" evidence="6">
        <location>
            <position position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR006621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR006621-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA06070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="340" mass="37137" checksum="A3649DFF572136E0" modified="2005-10-11" version="1">MQLNQPHIFSVAPMMEWTDRHCRVFHRLLTRRAWLYSEMLTTGAVIHGARDRLLGFSDIEHPVALQLGGSNPADLAIAARIGEDFGYDEINLNVGCPSDRVKDGRFGACLMAEPDIVAESVNAMKRAVSVPVTVKCRIGIDDQDMESALDIFVRKVVAAGVDALIVHARKAWLQGLSPKENRNVPPLDHGRVYRLKASFPGLPIIINGGIGGIPEAKQHLAHVDGVMLGRAAYQEPWRLLEVDSELFGEAPPHPTMKQALEAMVPYIEEQLAGGARLHSISRHLVGAFHGVPGARGFRRYLAENGVKPDAGVEVLQEAIARVDEFPAYSVKADAGFAVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNS4</accession>
    <name>Q3SNS4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2814</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316012.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2814"/>
    <dbReference type="EnsemblBacteria" id="ABA06067">
        <property type="protein sequence ID" value="ABA06067"/>
        <property type="gene ID" value="Nwi_2814"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2814"/>
    <dbReference type="eggNOG" id="COG3656">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129911_0_0_5"/>
    <dbReference type="OMA" id="EGTKWLK"/>
    <dbReference type="OrthoDB" id="2021063at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014469">
        <property type="entry name" value="DUF2271"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10029">
        <property type="entry name" value="DUF2271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF014995">
        <property type="entry name" value="UCP014995"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229058" evidence="2">
        <location>
            <begin position="20"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="170" mass="18699" checksum="EFC852F3DF6723DB" modified="2005-10-11" version="1">MRLLISAALTTLAASSSLASEATISIDVPQLDVAEYHKPYVAMWVEQADGGKITNLAVWYDVNHKKNEGTKWLKDVRQWWRRTGRELALPVDGLSSPTRAPGTHQIKFDSASKPLEGLTPGKYQLVIEAAREVGGRELLKIPFEWPPKAPATGEAKGERELGTVTLKLTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNT8</accession>
    <name>Q3SNT8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 18">Succinate dehydrogenase flavoprotein subunit</fullName>
            <ecNumber evidence="7 18">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ordered locus">Nwi_2800</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>a quinone + succinate = a quinol + fumarate</text>
            <dbReference type="Rhea" id="RHEA:40523"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 18">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5 18">Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (bacterial route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 18">Cell inner membrane</location>
            <topology evidence="4 18">Peripheral membrane protein</topology>
            <orientation evidence="4 18">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 18">Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="7 18"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315998.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2800"/>
    <dbReference type="EnsemblBacteria" id="ABA06053">
        <property type="protein sequence ID" value="ABA06053"/>
        <property type="gene ID" value="Nwi_2800"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2800"/>
    <dbReference type="eggNOG" id="COG1053">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014312_6_1_5"/>
    <dbReference type="OMA" id="DPIPIQP"/>
    <dbReference type="OrthoDB" id="153138at2"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER01005"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003952">
        <property type="entry name" value="FRD_SDH_FAD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037099">
        <property type="entry name" value="Fum_R/Succ_DH_flav-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030664">
        <property type="entry name" value="SdhA/FrdA/AprA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011281">
        <property type="entry name" value="Succ_DH_flav_su_fwd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014006">
        <property type="entry name" value="Succ_Dhase_FrdA_Gneg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632">
        <property type="entry name" value="PTHR11632"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46977">
        <property type="entry name" value="SSF46977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="SSF56425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01816">
        <property type="entry name" value="sdhA_forward"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01812">
        <property type="entry name" value="sdhA_frdA_Gneg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00504">
        <property type="entry name" value="FRD_SDH_FAD_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="18" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13 18" id="KW-0249">Electron transport</keyword>
    <keyword evidence="12 18" id="KW-0274">FAD</keyword>
    <keyword evidence="11 18" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="15 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="14 18 21" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 18" id="KW-0813">Transport</keyword>
    <keyword evidence="10 18" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="FAD_binding_2" evidence="19">
        <location>
            <begin position="24"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="Succ_DH_flav_C" evidence="20">
        <location>
            <begin position="474"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="16">
        <location>
            <position position="301"/>
        </location>
    </feature>
    <feature type="modified residue" description="Tele-8alpha-FAD histidine" evidence="17">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00008040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00019965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU362051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ABA06053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="607" mass="66259" checksum="04FA903433C9F58A" modified="2005-10-11" version="1">MAGNGNAAAAVNGKAYPIEDHTYDVVVVGAGGAGLRAVVGCGEAGLRTACITKVFPTRSHTVAAQGGISASLGNMHKDDWRWHMYDTVKGSDWLGDQDCIEYMVRNAPEAVYELEHWGVPFSRTEDGRIYQRPFGGMTIDYGKSQAQRTCAAADRTGHAMLHTMYGQALRRAAEFFIEFFAIDLIMDDQGACRGVIALKLDDGTLHRFRAQTTILATGGYGRVYASCTSAHICTGDGGGMALRAGLPLQDMEFVQFHPTGIYGSGCLVTEGARGEGGYLVNAEGERFMERYAPSAKDLASRDVVSRAMTIEIREGRGVGKKKDHIFLHLDHLDPKVLHERLPGISETVKIFAGVDVTREPIPILPTAHYNMGGIPTNFHGEVVTKKDGNDNAVVPGLMAVGEAACVSVHGANRLGSNSLIDLVVFGRAAARRCAEKLTANAEQPDLPANSSDMALGRLDKFRYAAGGTPTARLRENMQRVMQANCAVFRTQDTLAEGQKLIHQVHEGISDLAVFDRSLVWNTDLMETMEYDNLISQAVVTMDCAANRTESRGAHAREDYPQRDDQNWMKHTLARLSDRGVTTIDYRPVHDYTMTNDVQSIPPKARVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNW2</accession>
    <name>Q3SNW2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Diaminopimelate epimerase</fullName>
            <shortName evidence="8">DAP epimerase</shortName>
            <ecNumber evidence="4 8">5.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">PLP-independent amino acid racemase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">dapF</name>
        <name evidence="10" type="ordered locus">Nwi_2776</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>(2S,6S)-2,6-diaminoheptanedioate = meso-2,6-diaminoheptanedioate</text>
            <dbReference type="Rhea" id="RHEA:15393"/>
            <dbReference type="ChEBI" id="CHEBI:57609"/>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
            <dbReference type="EC" id="5.1.1.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-2,6-diaminopimelate from LL-2,6-diaminopimelate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the diaminopimelate epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.1.7" evidence="4 8"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2776"/>
    <dbReference type="EnsemblBacteria" id="ABA06029">
        <property type="protein sequence ID" value="ABA06029"/>
        <property type="gene ID" value="Nwi_2776"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2776"/>
    <dbReference type="eggNOG" id="COG0253">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053306_1_0_5"/>
    <dbReference type="OMA" id="HVAMRVH"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00025"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008837">
        <property type="term" value="F:diaminopimelate epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00197">
        <property type="entry name" value="DAP_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018510">
        <property type="entry name" value="DAP_epimerase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001653">
        <property type="entry name" value="DAP_epimerase_DapF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31689">
        <property type="entry name" value="PTHR31689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01678">
        <property type="entry name" value="DAP_epimerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00652">
        <property type="entry name" value="DapF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01326">
        <property type="entry name" value="DAP_EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 8" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="86"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="224"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="234"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Could be important to modulate the pK values of the two catalytic cysteine residues" evidence="8">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <feature type="site" description="Could be important to modulate the pK values of the two catalytic cysteine residues" evidence="8">
        <location>
            <position position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA06029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="297" mass="32397" checksum="403AEB9005A548AF" modified="2005-10-11" version="1">MIRKLEPMSVLANHAFSKMNGIGNEIVVVDLRDNPATVTAGEARAVASPGGVPYDQLMVLQPPRTPGTEAFVRIYNNDGSEANACGNGMRCVARQIFEATDKTALTFETRAGLLNCWRGSAPGLYSVDMGSPKFGWKDIPLAEEFRDTRYIELQVGPIDAPVLHSPSVVSMGNPHAIFWVDDIHAYDLARFGPLLENHPVFPERANITLAHIVDRDHITMRTWERGAGLTKACGSAACATAVAAARLKRVERIVHMTLPGGSLTIEWRERDDHVLMTGPAIFEYEGAFDPALFASVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP38</accession>
    <name>Q3SP38_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NUDIX hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2700</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315899.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2700"/>
    <dbReference type="EnsemblBacteria" id="ABA05953">
        <property type="protein sequence ID" value="ABA05953"/>
        <property type="gene ID" value="Nwi_2700"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2700"/>
    <dbReference type="eggNOG" id="COG1051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037162_20_2_5"/>
    <dbReference type="OMA" id="RCTPLYC"/>
    <dbReference type="OrthoDB" id="1846332at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="2">
        <location>
            <begin position="6"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="138" mass="14952" checksum="3A579D80602AEA69" modified="2005-10-11" version="1">MPPPQFPQIAVSAAIFRDGKVLLVRRARPPAKGLHSLPGGRIAFGETVAAALHREVAEETGLRIEITGLAGWREVMPTLPGDGHYIVMSFAARWIAGEPILNDELDAFSWVAPELPGELRLTEGLRTIIDAARRLVGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP45</accession>
    <name>Q3SP45_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Phosphoribulokinase</fullName>
            <ecNumber evidence="3 5">2.7.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2693</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>ATP + D-ribulose 5-phosphate = ADP + D-ribulose 1,5-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19365"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57870"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Carbohydrate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the phosphoribulokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.19" evidence="3 5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315892.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2693"/>
    <dbReference type="EnsemblBacteria" id="ABA05946">
        <property type="protein sequence ID" value="ABA05946"/>
        <property type="gene ID" value="Nwi_2693"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2693"/>
    <dbReference type="eggNOG" id="COG3954">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_962223_0_0_5"/>
    <dbReference type="OMA" id="TDILFYE"/>
    <dbReference type="OrthoDB" id="1793376at2"/>
    <dbReference type="UniPathway" id="UPA00655">
        <property type="reaction ID" value="UER00711"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008974">
        <property type="term" value="F:phosphoribulokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006082">
        <property type="entry name" value="PRK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00478">
        <property type="entry name" value="PHRIBLKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00567">
        <property type="entry name" value="PHOSPHORIBULOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PRK" evidence="6">
        <location>
            <begin position="7"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="290" mass="33013" checksum="55745A7929D3DA99" modified="2005-10-11" version="1">MSRKHPIISITGSSGAGTTSVKRTFEQIFRREKVVAAYIEGDAFHRYNRAEMRTRMAEESDKGNRHFSHFSPETNLFAELESVFRSYGETGTGTTRYYVHDDTESALHGVPPGTFTDWQPLPENSDLLFYEGLHGAVVTDKVNVAQYADLKIGVVPVINLEWIQKLHRDRSARGYSTEAVTDTILRRMPDYVNYICPQFAETDINFQRVPTVDTSNPFIARWIPTPDESMVVIRLKNPKGIDFPYLLSMIPNSFMSRANSIVIHGSKLDLAMQLILTPLILQLIERKRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP79</accession>
    <name>Q3SP79_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Multisubunit potassium/proton antiporter, PhaG subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2659</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315862.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2659"/>
    <dbReference type="EnsemblBacteria" id="ABA05912">
        <property type="protein sequence ID" value="ABA05912"/>
        <property type="gene ID" value="Nwi_2659"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2659"/>
    <dbReference type="eggNOG" id="COG1320">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121334_1_0_5"/>
    <dbReference type="OMA" id="HMLAKAA"/>
    <dbReference type="OrthoDB" id="2051355at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005451">
        <property type="term" value="F:monovalent cation:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098662">
        <property type="term" value="P:inorganic cation transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005133">
        <property type="entry name" value="PhaG_MnhG_YufB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34703">
        <property type="entry name" value="PTHR34703"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03334">
        <property type="entry name" value="PhaG_MnhG_YufB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01300">
        <property type="entry name" value="CPA3_mnhG_phaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="119" mass="12456" checksum="AA1A46913D8F280C" modified="2005-10-11" version="1">MNAADELSALPAVLTAVFLVGGAAITLIGSLGLLTLRSFYERVHAPTLGTTLGTACIAIASMIYFSALGTRPVLHELLIIVLIITTTPIGLMILVNAALVRDKSESDGAPQRKAKDEQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP81</accession>
    <name>Q3SP81_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Multisubunit potassium/proton antiporter, PhaE subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_2657</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CPA3 antiporters (TC 2.A.63) subunit E family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315860.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2657"/>
    <dbReference type="EnsemblBacteria" id="ABA05910">
        <property type="protein sequence ID" value="ABA05910"/>
        <property type="gene ID" value="Nwi_2657"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2657"/>
    <dbReference type="eggNOG" id="COG1863">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086615_4_0_5"/>
    <dbReference type="OMA" id="HAMDIED"/>
    <dbReference type="OrthoDB" id="1987527at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009521">
        <property type="term" value="C:photosystem"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016168">
        <property type="term" value="F:chlorophyll binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009767">
        <property type="term" value="P:photosynthetic electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002758">
        <property type="entry name" value="Cation_antiport_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036001">
        <property type="entry name" value="PS_II_antenna-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34584">
        <property type="entry name" value="PTHR34584"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01899">
        <property type="entry name" value="MNHE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019239">
        <property type="entry name" value="MrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161077">
        <property type="entry name" value="SSF161077"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="5"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="59"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="162" mass="17985" checksum="B45EF24E6DFE0E0A" modified="2005-10-11" version="1">MTRLVPFPLVSLSLLGFWLLLNETLSPGHILLGGVVALIGGWGLAALQPPKARPRRLGAIFRLAFLVFVDIVRSNVAVARIIFGPERRERTVGFVEIPLELRNPYGLAALACIITSTPGTLWVDFNEASGILTIHVLDLVDKAEWVDIIKGRYERRLMEIFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPB4</accession>
    <name>Q3SPB4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NUDIX hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2624</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Nudix hydrolase family. PCD1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315827.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2624"/>
    <dbReference type="EnsemblBacteria" id="ABA05877">
        <property type="protein sequence ID" value="ABA05877"/>
        <property type="gene ID" value="Nwi_2624"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2624"/>
    <dbReference type="eggNOG" id="COG0494">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040940_5_1_5"/>
    <dbReference type="OMA" id="YYIWGAT"/>
    <dbReference type="OrthoDB" id="1623169at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010945">
        <property type="term" value="F:CoA pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009132">
        <property type="term" value="P:nucleoside diphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03426">
        <property type="entry name" value="CoAse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045121">
        <property type="entry name" value="CoAse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000059">
        <property type="entry name" value="NUDIX_hydrolase_NudL_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12992">
        <property type="entry name" value="PTHR12992"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01293">
        <property type="entry name" value="NUDIX_COA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="4">
        <location>
            <begin position="59"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="221" mass="24570" checksum="5D7315F676CE6EE6" modified="2005-10-11" version="1">MVRTGAAATTIGAGEFFDRARERLRFDIPPGLTDAGIVPLTGDDDNDAVLRTIALETPIRPAAVLIPVIAREQPTVLLTQRAAHLKEHAGQIAFPGGKIDATDASPIDAALREAWEEVGLTRDFVEPIGYLDLYGTSFGFRILPTLARVRPGFDLRINTNEVDHAFEVPLSFLMNPLNHKLGRKQFRGRTRSFYEMPFAERNIWGATAGILRVLYERVYLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPF1</accession>
    <name>Q3SPF1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycine--tRNA ligase alpha subunit</fullName>
            <ecNumber evidence="9">6.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Glycyl-tRNA synthetase alpha subunit</fullName>
            <shortName evidence="9">GlyRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">glyQ</name>
        <name evidence="10" type="ordered locus">Nwi_2587</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly)</text>
            <dbReference type="Rhea" id="RHEA:16013"/>
            <dbReference type="Rhea" id="RHEA-COMP:9664"/>
            <dbReference type="Rhea" id="RHEA-COMP:9683"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.14"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.14" evidence="9"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315790.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2587"/>
    <dbReference type="EnsemblBacteria" id="ABA05840">
        <property type="protein sequence ID" value="ABA05840"/>
        <property type="gene ID" value="Nwi_2587"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2587"/>
    <dbReference type="eggNOG" id="COG0752">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057066_1_0_5"/>
    <dbReference type="OMA" id="SYYQFQV"/>
    <dbReference type="OrthoDB" id="676868at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006426">
        <property type="term" value="P:glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00733">
        <property type="entry name" value="GlyRS_alpha_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00254">
        <property type="entry name" value="Gly_tRNA_synth_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006194">
        <property type="entry name" value="Gly-tRNA-synth_heterodimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002310">
        <property type="entry name" value="Gly-tRNA_ligase_asu"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02091">
        <property type="entry name" value="tRNA-synt_2e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01044">
        <property type="entry name" value="TRNASYNTHGA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00388">
        <property type="entry name" value="glyQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50861">
        <property type="entry name" value="AA_TRNA_LIGASE_II_GLYAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA05840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="314" mass="35632" checksum="74F08FF7F68E5B84" modified="2005-10-11" version="1">MDPLPPHMRPERSFQGLILTLQRYWADYGCVILQPYDMEVGAGTFHPATTLRALGPKHWNAAYVQPSRRPKDGRYGENPNRLQHYYQFQVILKPSPPDIQDLYLKSLAAIGVDSALHDIRFVEDDWESPTLGAWGLGWECWCDGMEVSQFTYFQQVAGVECAPVAGELTYGLERLAMFVQGVDRVYDLNFNGREGAEKVTYGDVFLQAEQEYSRHNFEHSDTAMLFEQFRMAENACKTYLDAGWRDDPKQPGQRAAHLMALPAYDQCIKASHVFNLLDARGVISVTERQSYILRVRELAKACGDAWLHTDAGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPG6</accession>
    <name>Q3SPG6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2572</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315776.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05825">
        <property type="protein sequence ID" value="ABA05825"/>
        <property type="gene ID" value="Nwi_2572"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2572"/>
    <dbReference type="eggNOG" id="ENOG5032VZQ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131957_0_0_5"/>
    <dbReference type="OMA" id="WGLASFQ"/>
    <dbReference type="OrthoDB" id="1758645at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="171" mass="17921" checksum="1793AC986CA3CCA8" modified="2005-10-11" version="1">MVRSDGGRAALNLGAAAALAVFLGGCGSIGSIGLSPQPAQPPQPPVEPELPASIRAEELVGRWGLASYQNPNDRARTEAAAKSQCRNPYVISAGGNGGVIMHLADEATPQELRLKGSQSGKNYIGPEGPPGGPKDREIVSFDGRVLVTRFIDPDAATRYGNMVYIRCAPKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="135" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPK5</accession>
    <name>Q3SPK5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Histidine--tRNA ligase</fullName>
            <ecNumber evidence="9">6.1.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Histidyl-tRNA synthetase</fullName>
            <shortName evidence="9">HisRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">hisS</name>
        <name evidence="12" type="ordered locus">Nwi_2533</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>ATP + L-histidine + tRNA(His) = AMP + diphosphate + H(+) + L-histidyl-tRNA(His)</text>
            <dbReference type="Rhea" id="RHEA:17313"/>
            <dbReference type="Rhea" id="RHEA-COMP:9665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78527"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.21"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.21" evidence="9"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2533"/>
    <dbReference type="EnsemblBacteria" id="ABA05786">
        <property type="protein sequence ID" value="ABA05786"/>
        <property type="gene ID" value="Nwi_2533"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2533"/>
    <dbReference type="eggNOG" id="COG0124">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025113_3_2_5"/>
    <dbReference type="OMA" id="CDFDFIG"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004821">
        <property type="term" value="F:histidine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006427">
        <property type="term" value="P:histidyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00773">
        <property type="entry name" value="HisRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00859">
        <property type="entry name" value="HisRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00127">
        <property type="entry name" value="His_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015807">
        <property type="entry name" value="His-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004516">
        <property type="entry name" value="HisRS/HisZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033656">
        <property type="entry name" value="HisRS_anticodon"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001549">
        <property type="entry name" value="His-tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00442">
        <property type="entry name" value="hisS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="11">
        <location>
            <begin position="56"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="510"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA05786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="547" mass="60074" checksum="39CB91380CDE9A4F" modified="2005-10-11" version="1">MLADKSALLVRFSAGTTRAMRDTSNIGKKPKKPQKLRARLPRGLEDRGPAAIAATRRMVEKIRAVYERYGFEPVETPAFEYTDALGKFLPDQDRPNEGVFSFQDDDEQWISLRYDLTAPLARYVAENFQNLALPYRSYRAGYVYRNEKPGPGRFRQFMQFDADTVGSASPAADAEMCMMAADTMEALGIPRGQYVVKVNNRKVLDGVLESIGLGGEENAGRRLTVLRAIDKTDKFPEYEIRKLLGTGRWDGGEEGKGDFTKGAGLDERQSEVILRATSPANVAGRFNADGSGGVNNRDTVELLRSIAPAEILRQGCDELWEIAELLEAGGYGATSRNPDVRVIIDPSVVRGLEYYTGPVFEVELLLKTKDEKGRPVRFGSVGGGGRYDDLVSRFRGEPVPATGFSIGVSRLQAALTMLGKLDTKPQPGPVVVTVFGGDIAGYQKMVADLRNAGIRAELYLGNPKHSLGQQMKYADKRNSPCAIIQGTDEKANGKVLIKDLIAGAGLTSIEDRAEYLKKQAEAQVEAAESNLVAEVRKLLARHDVRWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPL5</accession>
    <name>Q3SPL5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Molybdopterin synthase catalytic subunit</fullName>
            <ecNumber evidence="4">2.8.1.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">MPT synthase subunit 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Molybdenum cofactor biosynthesis protein E</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Molybdopterin-converting factor large subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Molybdopterin-converting factor subunit 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_2523</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Converts molybdopterin precursor Z into molybdopterin. This requires the incorporation of two sulfur atoms into precursor Z to generate a dithiolene group. The sulfur is provided by MoaD.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-NH-CH2-C(O)SH + cyclic pyranopterin phosphate + H2O = 2 [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + 4 H(+) + molybdopterin</text>
            <dbReference type="Rhea" id="RHEA:26333"/>
            <dbReference type="Rhea" id="RHEA-COMP:12160"/>
            <dbReference type="Rhea" id="RHEA-COMP:12202"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58698"/>
            <dbReference type="ChEBI" id="CHEBI:59648"/>
            <dbReference type="ChEBI" id="CHEBI:90619"/>
            <dbReference type="ChEBI" id="CHEBI:90778"/>
            <dbReference type="EC" id="2.8.1.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterotetramer of 2 MoaD subunits and 2 MoaE subunits. Also stable as homodimer. The enzyme changes between these two forms during catalysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MoaE family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.12" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315727.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2523"/>
    <dbReference type="EnsemblBacteria" id="ABA05776">
        <property type="protein sequence ID" value="ABA05776"/>
        <property type="gene ID" value="Nwi_2523"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2523"/>
    <dbReference type="eggNOG" id="COG0314">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089568_2_1_5"/>
    <dbReference type="OMA" id="WPLQRVS"/>
    <dbReference type="OrthoDB" id="1877633at2"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030366">
        <property type="term" value="F:molybdopterin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00756">
        <property type="entry name" value="MoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036563">
        <property type="entry name" value="MoaE_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003448">
        <property type="entry name" value="Mopterin_biosynth_MoaE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02391">
        <property type="entry name" value="MoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54690">
        <property type="entry name" value="SSF54690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA05776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="155" mass="16945" checksum="9B7E2845C766AEB2" modified="2005-10-11" version="1">MTVPITIRLQEADFDLAHEVAALSRGRTDIGAVVTFSGICRGDEDGETIAALTLEHYPGMAEAEIARHAEEAVSRWPLTGITVIHRYGRIVPGENIVLVVTASSHREAAFQGAEFLMDYLKTKAPFWKREEGASGSGWVDAHDRDDTAAARWAKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPN8</accession>
    <name>Q3SPN8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2500</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315704.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2500"/>
    <dbReference type="EnsemblBacteria" id="ABA05753">
        <property type="protein sequence ID" value="ABA05753"/>
        <property type="gene ID" value="Nwi_2500"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2500"/>
    <dbReference type="eggNOG" id="ENOG50344KB">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1804703_0_0_5"/>
    <dbReference type="OMA" id="TRMIADN"/>
    <dbReference type="OrthoDB" id="1710609at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="152" mass="16115" checksum="4D64183677B7C0A0" modified="2005-10-11" version="1">MNATPSETAETSATETDSNLAAVSEVEAGIRDFVRNDIAYLRRPAAGAAPAAGAPPAASAPNADVKLEPSTEATVNNVNSLIQRVAGTSLAEIENLVTELESLRDLLHAEGQRVQREISGYAQLSQAAMKSTRIIADNVAQWKRTAENLRSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPT1</accession>
    <name>Q3SPT1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2457</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05710">
        <property type="protein sequence ID" value="ABA05710"/>
        <property type="gene ID" value="Nwi_2457"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2457"/>
    <dbReference type="HOGENOM" id="CLU_3120393_0_0_5"/>
    <dbReference type="OMA" id="RLWRSSH"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="50" mass="5538" checksum="D965756991257887" modified="2005-10-11" version="1">MTDLHLGRPNRGGVEDMRGIVPEEQNYAIGWTLSAFLTLSVVGVVWTFGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPT2</accession>
    <name>Q3SPT2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2456</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2456"/>
    <dbReference type="EnsemblBacteria" id="ABA05709">
        <property type="protein sequence ID" value="ABA05709"/>
        <property type="gene ID" value="Nwi_2456"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2456"/>
    <dbReference type="eggNOG" id="ENOG5032YAU">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145985_1_0_5"/>
    <dbReference type="OMA" id="MEKSARW"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="103" mass="12158" checksum="2C81EB35AD78934A" modified="2005-10-11" version="1">MSKDHRAELDRHLAWFEGKLPRRFARFIGWVRKPSSRLVRIPLAILLIIGGIFSFLPVLGLWMLPLGLILIAQDLPFLQRPMARMLGWIERKWLERKAAKRFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPU0</accession>
    <name>Q3SPU0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Methyltransf_25 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2448</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2448"/>
    <dbReference type="EnsemblBacteria" id="ABA05701">
        <property type="protein sequence ID" value="ABA05701"/>
        <property type="gene ID" value="Nwi_2448"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2448"/>
    <dbReference type="eggNOG" id="COG0500">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1208797_0_0_5"/>
    <dbReference type="OMA" id="NSVAQYM"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransf_25" evidence="2">
        <location>
            <begin position="86"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="284" mass="30803" checksum="1449C9F911EFB235" modified="2005-10-11" version="1">MGQFRCAAESPFRPRKTRSTTSSLTPDSASGSGRGTQRLPGPSMDDWIAFYDSAHTIYVSKLHRDAHFRTIARNIVSYIPSRDSVVLDYACGEALSAETVADACGRLILTEPAPGVRGRLVTRFTPNTKIDVRSLDELRHMDEESIDLAVMNSVAQYMTPDELDAGFAVVRRLLKPTGLLVLGDIPQPHIGLAGDVIALLGFAAKHGFVKDALRGLVRTAMSDYPQLRLRIGLQRYSEAEMLAKLAAAGMTAIRAPVNIGHNASRMTFLAGRPVKADRGATAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPU3</accession>
    <name>Q3SPU3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2445</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315653.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2445"/>
    <dbReference type="EnsemblBacteria" id="ABA05698">
        <property type="protein sequence ID" value="ABA05698"/>
        <property type="gene ID" value="Nwi_2445"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2445"/>
    <dbReference type="eggNOG" id="COG1610">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079430_2_2_5"/>
    <dbReference type="OMA" id="AMGAVMK"/>
    <dbReference type="OrthoDB" id="1498741at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016884">
        <property type="term" value="F:carbon-nitrogen ligase activity, with glutamine as amido-N-donor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1510.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023168">
        <property type="entry name" value="GatB_Yqey_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019004">
        <property type="entry name" value="YqeY/Aim41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042184">
        <property type="entry name" value="YqeY/Aim41_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28055">
        <property type="entry name" value="PTHR28055"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09424">
        <property type="entry name" value="YqeY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="SSF89095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="151" mass="16249" checksum="103B08D14A571286" modified="2005-10-11" version="1">MLRDDINNAVKEAMKARDERRLSTLRMVNSTIKNADIEARGQGKPPLSDGDLLGLLQKMIKQRQESVELYEKGGREELAAQERAEIAVISTYLPKQMSDEDVKAAVAAIIADTGASGIKDMGKVIGALKARYAGQMDFGKASGVVKAALTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPY8</accession>
    <name>Q3SPY8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2399</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2399"/>
    <dbReference type="EnsemblBacteria" id="ABA05653">
        <property type="protein sequence ID" value="ABA05653"/>
        <property type="gene ID" value="Nwi_2399"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2399"/>
    <dbReference type="eggNOG" id="ENOG503422C">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_747533_0_0_5"/>
    <dbReference type="OMA" id="YAPKTER"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008775">
        <property type="entry name" value="Phytyl_CoA_dOase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05721">
        <property type="entry name" value="PhyH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="385" mass="43405" checksum="929750299A15A86E" modified="2005-10-11" version="1">MMTTFDPPADTLPDLASGNIVSPPVPAYRSSSLPDIDYDAHPAYGGILPAATLSERFHALRVFAKSFAFVTIRRIVDYENMPLLSKEAGGGHLNLSSATRYLTMFASIRAQRFLRTLIGTRRAAKPTNETLFQRMKSDGVACMTLSPEEMENIRQVIAPCFAHLDERRAAIPVERRKFDDNVYWCTRSRESKVFETIEEVFRRHGITDVASAYLGRPVRVKHVNPQINDDDYTFWKKQFADTDVPDPWSTYLHIDATYGMLKCALYIHDIGPDGGPFCYVRGSHRIKVGWFEGMIRRTNDFCGFSGRKPEARKKFMALPRMLRKKADFGADVLDDSAASKSVREGHFAATSNYGNCVLFDGHGIHRGGMVNKGERRCIFVLLAEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPZ9</accession>
    <name>Q3SPZ9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Aminotransferase</fullName>
            <ecNumber evidence="3">2.6.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2388</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315603.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2388"/>
    <dbReference type="EnsemblBacteria" id="ABA05642">
        <property type="protein sequence ID" value="ABA05642"/>
        <property type="gene ID" value="Nwi_2388"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2388"/>
    <dbReference type="eggNOG" id="COG0001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_1_4_5"/>
    <dbReference type="OMA" id="GYNGWHD"/>
    <dbReference type="OrthoDB" id="493793at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="1 2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="437" mass="47657" checksum="46E8AFFFE47AD80E" modified="2005-10-11" version="1">MVDTGQTLYRRAREIIPGGTQLLSKRPEMFLPDQWPTYYRSAKGAEVVDLDGRTLLDMSYCGIGATVLGFADPDVNAAVKTAIDLGAMSTLNCGEEVELAELLVELHPWADMVRFGRSGGEALAVAIRIARATTGRDMIAFCGYHGWHDWYLSANLGETTALDGHLLPGLKTAGVPRGLAGLMHPFHFNKVNEIEAIISEHGSRLAAIVMEPVRSREPTPEFILGVRRLADQCGAVLIFDEVTSGLRINSGGAHLAYNVDPDVAVFAKALANGYPMAAIIGRRAVMDAAQDTFVSSTAWTERIGPVAALATLRKHRAQNVAAHLVRIGQRIQKGWEDASSAARLPLHVSGIPPLGHFAFDLPNAQEVRTLFTQMMLDRGILATGSFYAMWTHTDEHVDRYLEAVNQVFRDLRLAIDQNAITQLLRGPVAHSGFSRLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ64</accession>
    <name>Q3SQ64_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ABC-type Fe3+-siderophore transport system permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_2323</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315542.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2323"/>
    <dbReference type="EnsemblBacteria" id="ABA05577">
        <property type="protein sequence ID" value="ABA05577"/>
        <property type="gene ID" value="Nwi_2323"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2323"/>
    <dbReference type="eggNOG" id="COG0609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013016_0_3_5"/>
    <dbReference type="OMA" id="MQGVFGN"/>
    <dbReference type="OrthoDB" id="1283908at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000522">
        <property type="entry name" value="ABC_transptr_permease_BtuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472">
        <property type="entry name" value="PTHR30472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01032">
        <property type="entry name" value="FecCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="25"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="174"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="271"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="310"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="338"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA05577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="365" mass="37179" checksum="AD1244A8F4A94CFA" modified="2005-10-11" version="1">MTAIDSIGNAESSRGSASARPAPTLLFGCLLVALSGVAIAAVTMGAAGIPLGRVPVALGLWTSEQAGALLARDQLVLWSIRIPRILAAAVVGGLLATSGAIMQGLFRNPLADPALVGVSSGGAFAAASAIVLIDSSYGDAFRFMQNAILPLAAFLGSLVTTFVLYGIASRMGRTSIALFLLAGLAIAAIANAGLGLLVFIADDRQLRDITFWMLGSLSGATWSKAVTIAPVLVIGVFACFRIGRGLDLLVLGESEAFHSGVNVERLKQASIVLISAMTGVAVSICGVVGFIGIVVPHLLRLMIGPGHRMLLPASALLGAILVVGADTLARTIVAPAEMPIGILTAAIGAPFFLAILLRQRGLAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ82</accession>
    <name>Q3SQ82_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Biopolymer transport protein ExbB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">Nwi_2305</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Involved in the TonB-dependent energy-dependent transport of various receptor-bound substrates. Protects ExbD from proteolytic degradation and functionally stabilizes TonB.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The accessory proteins ExbB and ExbD seem to form a complex with TonB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the exbB/tolQ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2305"/>
    <dbReference type="EnsemblBacteria" id="ABA05559">
        <property type="protein sequence ID" value="ABA05559"/>
        <property type="gene ID" value="Nwi_2305"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2305"/>
    <dbReference type="eggNOG" id="COG0811">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053325_0_0_5"/>
    <dbReference type="OMA" id="DLSPWSM"/>
    <dbReference type="OrthoDB" id="1796180at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002898">
        <property type="entry name" value="MotA_ExbB_proton_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014164">
        <property type="entry name" value="TonB_ExbB_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01618">
        <property type="entry name" value="MotA_ExbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02797">
        <property type="entry name" value="exbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="106"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="213"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="256"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="MotA_ExbB" evidence="16">
        <location>
            <begin position="167"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="43"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="45"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ABA05559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="320" mass="32978" checksum="E777E89CA8850481" modified="2005-10-11" version="1">MMRHSGMTLSRKRHARVARAAIAGVALLIGAFGPLHAQGLPQATPAITAPHSPSGPPASEISSTTDHLNATSSSPAPASAPSAIIANDSASPHELSPWSMFMSADIVVQLVMIGLAFASLVTWTICIAKLAELSLARRALRAALNKIGDARSLAEAQMALGAREGVLASLLASAMHEARLSAGISSDAGIKERAASSFAEIVRAESRRIRLGMGLLATIGATSPFVGLFGTVWGIMNSFIGISKSQTTNLAVVAPGIAEALLATALGLVAAIPAVIIYNYFARVTRGYTELVGRASGAAGRLLSRDLDRTHGSLTSRAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQF9</accession>
    <name>Q3SQF9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2228</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315450.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05482">
        <property type="protein sequence ID" value="ABA05482"/>
        <property type="gene ID" value="Nwi_2228"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2228"/>
    <dbReference type="eggNOG" id="ENOG503322S">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_952750_0_0_5"/>
    <dbReference type="OrthoDB" id="1985987at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="274" mass="28825" checksum="22F5302C93442FA8" modified="2005-10-11" version="1">MSVLSIAGFGVFVTGLVTVAFGIPIKEFSFGDTMIVSGAVVSCTGLIMVSLSVVVRELQKVADHLNSGDRFGSGAVANHSGVHAGDVRVPIPPGRPVADSPPPLADGDRIAPDPRSDLRSAPSVLSQRRQGQAPERDHVPADRDRATVASVPDAEFPASSDQPSQPKPRRNLLFASTVREREQGRAERFGLSAPAVHAPDPETPKPASRGDQPADASDRNASPATVLRSGVVNGMAYSLYSDGAIDAQLPEGEVRFASIDQLRAHLDQGSRRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQM2</accession>
    <name>Q3SQM2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tim44 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2165</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315388.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2165"/>
    <dbReference type="EnsemblBacteria" id="ABA05419">
        <property type="protein sequence ID" value="ABA05419"/>
        <property type="gene ID" value="Nwi_2165"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2165"/>
    <dbReference type="eggNOG" id="COG4395">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066429_1_0_5"/>
    <dbReference type="OMA" id="WKLAAIQ"/>
    <dbReference type="OrthoDB" id="1536134at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007379">
        <property type="entry name" value="Tim44-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04280">
        <property type="entry name" value="Tim44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00978">
        <property type="entry name" value="Tim44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229061" description="Tim44 domain-containing protein" evidence="3">
        <location>
            <begin position="31"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="93"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Tim44" evidence="4">
        <location>
            <begin position="168"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="312" mass="32641" checksum="A51578A817058B52" modified="2005-10-11" version="1">MKLAKRASGLMKAFAVVLSLALPLMFAVSAADARIGGGKSMGSRGSHTFSAPPATSTAPNAARPFDRTMSQPGRPAAGAAAAGRSARPGMGMLGGLAAGFLGAGLLGMLFGGGLFGGLEGLSSIIGLVLQVGLIYLLVRFAMSWWQRRNAPAYAGPTPGSAAQTNARSGLGFGSVSNAAPLEITPSDYETFERLLGEIQAAWSKEDVNTLHTLATPEMVSYFTEDLRANDARGVVNTVSDVKLLQGDLAEAWREGVTDYATVALRYSLIDKTTERASGRIVDGGDQPQEAAEIWTFARRDGGKWELSAIQQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQS1</accession>
    <name>Q3SQS1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315343.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2115"/>
    <dbReference type="EnsemblBacteria" id="ABA05370">
        <property type="protein sequence ID" value="ABA05370"/>
        <property type="gene ID" value="Nwi_2115"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2115"/>
    <dbReference type="eggNOG" id="ENOG5033AXH">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154609_1_0_5"/>
    <dbReference type="OMA" id="ACAVESM"/>
    <dbReference type="OrthoDB" id="1960973at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="114" mass="12392" checksum="921A5BA5C1797ABF" modified="2005-10-11" version="1">MVGTSKHATSPSHLSTDDPHLDRLLNPAQFYKHPREVIDDPHLGLSEKRAILSSWASDACAVESMPALRQIPGTPAPVSFDDVMDALRRLDDLARPPARTPAFPADNTVCPPTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQV0</accession>
    <name>Q3SQV0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Divergent AAA region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2084</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2084"/>
    <dbReference type="EnsemblBacteria" id="ABA05341">
        <property type="protein sequence ID" value="ABA05341"/>
        <property type="gene ID" value="Nwi_2084"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2084"/>
    <dbReference type="eggNOG" id="COG2865">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1883576_0_0_5"/>
    <dbReference type="OMA" id="QDNDARA"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007421">
        <property type="entry name" value="Schlafen_AlbA_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038461">
        <property type="entry name" value="Schlafen_AlbA_2_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04326">
        <property type="entry name" value="AlbA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AlbA_2" evidence="1">
        <location>
            <begin position="15"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="135" mass="14621" checksum="F704131FA1F98F16" modified="2005-10-11" version="1">MASVDLSDLLAGTSENLGIEFKAWMDTSNGEVRAKLARHIAALANHGGGYLIFGVDDKTRKPLGKTEFNLDLFSQDAITGIIKKYLDPRIQVLVEHVVLEGTTYPVVVIPPHGARPVFAIADGPQDDRNRPVGVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQY0</accession>
    <name>Q3SQY0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">TonB like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_2053</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
            <orientation evidence="1">Periplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TonB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2053"/>
    <dbReference type="EnsemblBacteria" id="ABA05311">
        <property type="protein sequence ID" value="ABA05311"/>
        <property type="gene ID" value="Nwi_2053"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2053"/>
    <dbReference type="eggNOG" id="COG0810">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076333_2_2_5"/>
    <dbReference type="OMA" id="SEAQAYN"/>
    <dbReference type="OrthoDB" id="2074833at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006260">
        <property type="entry name" value="TonB/TolA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037682">
        <property type="entry name" value="TonB_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03544">
        <property type="entry name" value="TonB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01352">
        <property type="entry name" value="tonB_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="TonB_C" evidence="10">
        <location>
            <begin position="179"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="59"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA05311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="256" mass="27526" checksum="A08A779752C651D8" modified="2005-10-11" version="1">MQGGWMLRFIARRIPTCIALAAHTAVVFTLVYDPAIKLVIDEQPGPTFVEIVTVPPHGSTAAKQDEAAEPASTAPAELPLPALAEESPPEISDRGEETEKPVTLPVPNPKRAAVPKRSAPRVRLAARSAPPRTSEQLTDAAGTPEQLEGGATGTTDISQVPVDVLSAWKARLLSHLERYKRYPSAARKKGTEGTAYLSFGMDSEGRVLGYHLARSSGSSELDEEVLAMIVRASPLPPAPPEMNTQVKLIVPIRFRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR07</accession>
    <name>Q3SR07_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NnrU</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2025</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2025"/>
    <dbReference type="EnsemblBacteria" id="ABA05284">
        <property type="protein sequence ID" value="ABA05284"/>
        <property type="gene ID" value="Nwi_2025"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2025"/>
    <dbReference type="eggNOG" id="COG4094">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104582_1_0_5"/>
    <dbReference type="OMA" id="VKIWALA"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009915">
        <property type="entry name" value="NnrU_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07298">
        <property type="entry name" value="NnrU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="NnrU" evidence="2">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="189" mass="20270" checksum="233938341C1D7608" modified="2005-10-11" version="1">MIAGLVLSLGVHLVVTRREFRARLISSYGEGPYKIGFSLISAVGVALTLWGFLSYRATGVIDVWYPPRVMKHIAEALILPSIILVVASYIRGRIYAKLKHPMLAGIKLWATAHLLANGDLGSIILFGSILAWAVIDRISLKHRSDPGGLPIPVGGVRNDVIAIVVGVVVYVAIGALFHPFVLGVPVFGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR17</accession>
    <name>Q3SR17_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">DNA repair protein RadA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">radA</name>
        <name evidence="9" type="ordered locus">Nwi_2015</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">DNA-dependent ATPase involved in processing of recombination intermediates, plays a role in repairing DNA breaks. Stimulates the branch migration of RecA-mediated strand transfer reactions, allowing the 3' invading strand to extend heteroduplex DNA faster. Binds ssDNA in the presence of ADP but not other nucleotides, has ATPase activity that is stimulated by ssDNA and various branched DNA structures, but inhibited by SSB. Does not have RecA's homology-searching function.</text>
    </comment>
    <comment type="function">
        <text evidence="5">Plays a role in repairing double-strand DNA breaks, probably involving stabilizing or processing branched DNA or blocked replication forks.</text>
    </comment>
    <comment type="domain">
        <text evidence="5">The middle region has homology to RecA with ATPase motifs including the RadA KNRFG motif, while the C-terminus is homologous to Lon protease.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the RecA family. RadA subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315260.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2015"/>
    <dbReference type="MEROPS" id="S16.A04"/>
    <dbReference type="EnsemblBacteria" id="ABA05274">
        <property type="protein sequence ID" value="ABA05274"/>
        <property type="gene ID" value="Nwi_2015"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2015"/>
    <dbReference type="eggNOG" id="COG1066">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018264_0_1_5"/>
    <dbReference type="OMA" id="FELMRLA"/>
    <dbReference type="OrthoDB" id="505485at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000725">
        <property type="term" value="P:recombinational repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01498">
        <property type="entry name" value="RadA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004504">
        <property type="entry name" value="DNA_repair_RadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041166">
        <property type="entry name" value="Rubredoxin_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18073">
        <property type="entry name" value="Rubredoxin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00416">
        <property type="entry name" value="sms"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5 6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0346">Stress response</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RECA_2" evidence="8">
        <location>
            <begin position="68"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Lon-protease-like" evidence="5">
        <location>
            <begin position="354"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="457"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="RadA KNRFG motif" evidence="5">
        <location>
            <begin position="255"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="97"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="486" mass="50646" checksum="B193C1E14B8013DA" modified="2005-10-11" version="1">MAKSALSFVCQNCGAAYNRWQGKCDGCGEWNTLAEEDITGATTMPVSVRSRRKGRLFTLETLTGKTQDAPRLSSGMAELDRVTGGGFVRGSVLLVGGDPGIGKSTLLTQATSLLARSGHRAVYISGEEAVAQVRLRAERLGLADAPVQLAAETSVEDIVATLSEGTVPRLVVIDSIQTMWTDTVESAPGTVTQVRASAQALIRFAKKSGAALILVGHVTKDGQIAGPRVVEHMVDAVLSFEGEGSQQFRILRAVKNRFGPTDEIGVFEMTGLGLREVSNPSELFLSERDLGSPGTAVFAGIEGTRPVLVELQALVAPTSLGTPRRAVVGWDSSRLSMVLAVLEAHCGVKLSGHDVYFNVAGGLRINEPAADLAAAAALVSSLTNAQLPTDAVYFGEISLSGAVRPVAQTTARLKEAAKLGFGRAVLPESARGDIGGDAGLSLDAVAGLTTLVADIAARGQARPGSPGKRTDPEKNATPGQNRRQGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRC9</accession>
    <name>Q3SRC9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Bifunctional NAD(P)H-hydrate repair enzyme</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Nicotinamide nucleotide repair protein</fullName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName evidence="16">ADP-dependent (S)-NAD(P)H-hydrate dehydratase</fullName>
                <ecNumber evidence="16">4.2.1.136</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="16">ADP-dependent NAD(P)HX dehydratase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="16">NAD(P)H-hydrate epimerase</fullName>
                <ecNumber evidence="16">5.1.99.6</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="15" type="primary">nnrE</name>
        <name evidence="14" type="synonym">nnrD</name>
        <name evidence="18" type="ordered locus">Nwi_1902</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 16">Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="function">
        <text evidence="14">Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="function">
        <text evidence="15">Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15 16">
            <text>(6R)-NADHX = (6S)-NADHX</text>
            <dbReference type="Rhea" id="RHEA:32215"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:64075"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 15 16">
            <text>(6R)-NADPHX = (6S)-NADPHX</text>
            <dbReference type="Rhea" id="RHEA:32227"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:64077"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 14 16">
            <text>(6S)-NADHX + ADP = AMP + H(+) + NADH + phosphate</text>
            <dbReference type="Rhea" id="RHEA:32223"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 14 16">
            <text>(6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate</text>
            <dbReference type="Rhea" id="RHEA:32235"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 16">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="15 16">Binds 1 potassium ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the NnrE/AIBP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 16">In the C-terminal section; belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 16">In the N-terminal section; belongs to the NnrE/AIBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.136" evidence="16"/>
    <dbReference type="EC" id="5.1.99.6" evidence="16"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315158.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1902"/>
    <dbReference type="EnsemblBacteria" id="ABA05162">
        <property type="protein sequence ID" value="ABA05162"/>
        <property type="gene ID" value="Nwi_1902"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1902"/>
    <dbReference type="eggNOG" id="COG0062">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0063">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024853_4_1_5"/>
    <dbReference type="OMA" id="HNGGDAL"/>
    <dbReference type="OrthoDB" id="1748633at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052855">
        <property type="term" value="F:ADP-dependent NAD(P)H-hydrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052856">
        <property type="term" value="F:NADHX epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052857">
        <property type="term" value="F:NADPHX epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01171">
        <property type="entry name" value="YXKO-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01965">
        <property type="entry name" value="NADHX_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01966">
        <property type="entry name" value="NADHX_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017953">
        <property type="entry name" value="Carbohydrate_kinase_pred_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030677">
        <property type="entry name" value="Nnr"/>
    </dbReference>
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        <property type="entry name" value="yjeF_cterm"/>
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    </dbReference>
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    <keyword evidence="8 14 16" id="KW-0067">ATP-binding</keyword>
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        <location>
            <begin position="9"/>
            <end position="206"/>
        </location>
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            <begin position="55"/>
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        </location>
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        <location>
            <position position="56"/>
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        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
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        <location>
            <position position="116"/>
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        <location>
            <position position="149"/>
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        <location>
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        <ligand>
            <name>K(+)</name>
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        <location>
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        <location>
            <begin position="407"/>
            <end position="411"/>
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        <ligand>
            <name>AMP</name>
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        <location>
            <position position="436"/>
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        <ligand>
            <name>AMP</name>
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        </ligand>
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        <location>
            <position position="437"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
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            <dbReference type="ARBA" id="ARBA00000013"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00000909"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00001026"/>
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            <dbReference type="ARBA" id="ARBA00001241"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00006001"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009524"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00025153"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_01966"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR017184"/>
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    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51385"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ABA05162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="499" mass="51333" checksum="B08BAED4BAA768FC" modified="2005-10-11" version="1">MEILTTGEMEQADRLSIAGGVSGFALMLRAGQAVANAAADLAEKGPILVVVGCGNNGGDGFVAATELAARGHEVSVILLGERDSLKGDAALAAKGWKAPVLPCDLSALGSPALIIDALFGAGLNRPVKGDALATIEAIMANGAPVLSIDLPSGINGTTGAVMGAAIRATETVTFFRKKPAHLLLPGRIHCGRVRVADIGIADAVLDEIRPVTFENTPDYWNGAFPAPHIDGHKYKRGHAVVVSGHLTSTGAARLSARGALRAGAGLVTVLSPDDALGANAAALTAVMVRRMNGAAGFAEVLADRRLNACVIGPGAGIGEETRALVSTALAAGRALVLDADALTSFADTPDRLFETVRQSGGSDIILTPHEGEFHRLFSEMSNKHPFRSKLERVRDAARRSGCVVLLKGADTVVASPDGRATIAANAPPWLATAGSGDVLSGIIGGLLAQSVPAFEAACMGVWMHGETGREAGPGLIAEDLPEVLPAVLRRLYDRFGIDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRG4</accession>
    <name>Q3SRG4_NITWN</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ordered locus">Nwi_1867</name>
    </gene>
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        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
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            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
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                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_007406.1"/>
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    <dbReference type="EnsemblBacteria" id="ABA05127">
        <property type="protein sequence ID" value="ABA05127"/>
        <property type="gene ID" value="Nwi_1867"/>
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    <dbReference type="eggNOG" id="COG2847">
        <property type="taxonomic scope" value="Bacteria"/>
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    <dbReference type="HOGENOM" id="CLU_100939_2_2_5"/>
    <dbReference type="OMA" id="MKMQQVP"/>
    <dbReference type="OrthoDB" id="1872498at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007410">
        <property type="entry name" value="PCuAC"/>
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        <property type="entry name" value="PCuAC_sf"/>
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    <dbReference type="PANTHER" id="PTHR36302">
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
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        <location>
            <begin position="28"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="178" mass="18367" checksum="983FF2AB401CAD75" modified="2005-10-11" version="1">MKSWLTSRGMIGAVAVATLGLVTPVHAESVSAGDLVVSLPWTRATAGGAKVAVGYVTIENKGGVADKLVGGSTDAAGRLEVHEMSMNNGVMKMNQVEGGLAVEPGKTVKLEPGGYHLMLLDLKHPFKKGEKVPITLKFEKAGDVAVTLDVEGMGTRAPGASGGAMKNPPDHSHDGSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRI9</accession>
    <name>Q3SRI9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ComEC/Rec2-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_1842</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
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                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315098.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1842"/>
    <dbReference type="EnsemblBacteria" id="ABA05102">
        <property type="protein sequence ID" value="ABA05102"/>
        <property type="gene ID" value="Nwi_1842"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1842"/>
    <dbReference type="eggNOG" id="COG0658">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011826_1_0_5"/>
    <dbReference type="OMA" id="HPSFQMS"/>
    <dbReference type="OrthoDB" id="1796527at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004477">
        <property type="entry name" value="ComEC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025405">
        <property type="entry name" value="DUF4131"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03772">
        <property type="entry name" value="Competence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13567">
        <property type="entry name" value="DUF4131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00360">
        <property type="entry name" value="ComEC_N-term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="336"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="381"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="447"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="479"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="533"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="561"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4131" evidence="9">
        <location>
            <begin position="77"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Competence" evidence="8">
        <location>
            <begin position="274"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="703"/>
            <end position="778"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA05102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="778" mass="82316" checksum="026736AC327801F8" modified="2005-10-11" version="1">MAERGRARDRRGGYAGTWPTRQAVAAPSQTPSEALPAIVARLRAWANAEAAAVRLLPWVPVAFGAGIAIYFAADHEPVAWVVSVTAGALFVSALLLRRHRIFPAAVMVAAVAAGFAVATIKTAHVSHAVLTRPMYTPLLQGFVETREVRERTDRFILRVVRMDSRRPHVKLDRVRLSVRKGAAPAVGSFVELKARLQPPLTPVRPGSYDFARDMYFQGIGASGFVMGAIKTVASPPAGGIALRYAALMQELRDAIDARIRLVLSGDSRAIATALLTGRRDAITPPVNEAMFISGLGHVLSISGYHMAVVAGVVFFAVRALLALVPALTATFPIKKWSAAAALAAAAFYLLLSGAEVATQRSFFMTAAVLIAIMVDRRAITFRTLAVAAMIVLAIAPESLIHPSFQMSFAATLGLVALVQVGMPRMLATPDHSPTAKAALWGGREIAMLALASFVAGLATTPYAAFHFHRVTPYGMAANLLAMPVISALVMPAGLLGLAAMPFGFDGVFWRLMDFGIEWMVAVAQGVVELPGSIGRIAAFGTGPLLAATAGIVLLGLLRTPLRWGGAIVVGLAIVWSLAVKPPDILISAEGRNVAVRGGDGRLRLISTGKDSFTVREWLAADGDVRGPTDPTLAEGVSCDDAGCVVKASGGGIVALTRHPEALQDDCARAELIVTRWQAPEGCLAHVIDQNRLRREGALALWRTPNSSRQDASRLDVSRSGASKPGASRPGFAVDAVRPAGLKRPWSSAAFDEQPARPGSKPEPSVDATPDPEDLQPDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRU8</accession>
    <name>Q3SRU8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="13">TonB-dependent siderophore receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Nwi_1732</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the TonB-dependent receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1732"/>
    <dbReference type="EnsemblBacteria" id="ABA04993">
        <property type="protein sequence ID" value="ABA04993"/>
        <property type="gene ID" value="Nwi_1732"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1732"/>
    <dbReference type="eggNOG" id="COG4773">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008287_9_3_5"/>
    <dbReference type="OMA" id="GGLVFNY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015344">
        <property type="term" value="F:siderophore uptake transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055072">
        <property type="term" value="P:iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012910">
        <property type="entry name" value="Plug_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037066">
        <property type="entry name" value="Plug_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011662">
        <property type="entry name" value="Secretin/TonB_short_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039423">
        <property type="entry name" value="TonB-dep_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000531">
        <property type="entry name" value="TonB-dep_rcpt_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036942">
        <property type="entry name" value="TonB_rcpt_b-brl_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010917">
        <property type="entry name" value="TonB_rcpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010105">
        <property type="entry name" value="TonB_sidphr_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32552">
        <property type="entry name" value="PTHR32552"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07715">
        <property type="entry name" value="Plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00593">
        <property type="entry name" value="TonB_dep_Rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00965">
        <property type="entry name" value="STN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01783">
        <property type="entry name" value="TonB-siderophor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01156">
        <property type="entry name" value="TONB_DEPENDENT_REC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0410">Iron transport</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="6 10" id="KW-0798">TonB box</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="STN" evidence="12">
        <location>
            <begin position="77"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="157"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA04993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="864" mass="94289" checksum="21256E81385F1321" modified="2005-10-11" version="1">MRQGDLGQRALEYRFRGSAFCKDVRKALIAALLATTAVIGGSAAPQSQALAQAQTSFNIPAGPLNRVLAAFGRQSGIQLSYEASIASGKTSPGIQGAATREQAIARILQGSGLSYSFKDSRNVLITQPGATATGAAPAGAIALDTIDVEGATRPGRTEGTESYTTSQSSFGKGQSLRELPQTVTVMTHQRMRDQGLRTLEDVMEYTPGITVEQQSSSSSGFYSRGFEITNFQIDGNSPLLSRSYSQTYAGGLTTSQADLAMYDSVEVLRGSDALYGVSGAPGGVINLVRKKPTSQFQMNALARGGSWNNYRGELDVSGSLNANGAVRGRFVGVYEDREFFYNVAKSNKYLLYGIVEADVTDSTMLTFGSDIMRQDGSNWRYGLPRYSNGADIGLPRSTFLAPDENRWLRNTTKQFVRLDQELGDKWTLGIEASRAHSKVDEKTQVFWGAIDPITLSGLRSGTGHGRQFYRNETQSTLDAVLKGSFHLLGMDHKLILGANIIRYDSGGETNARNGGTIFVPNIFEFDPADFRLNDPYFRIFQWATKITQKGVYGSLAAKIADPLTLIVGGRLSWYNYDDAITTFDRNTGNITGTTLTRFDDNKVFTPSLGLVYDFSTHWSAYASFAETYQPQANSLSGPLPGTPLGPVTGRTYETGVKGSLLDGRLNTSIALYHVNRNGEAVPDPAYPPVTGDLGRNCCFLGQGRIVSRGVDAEVSGEIAEGWRVQAGYTFNDNENVVAGGRYSTVTPKHLFKLWTTYALRGPLDGWKVGGGVTAQSSFYRKGEARTLNPNTGEYDGPLAPFEFTEPGRAVVDLYVEYKINQHWSATLNVNNVFDKRYYQTMGYADYANWYGPPRNFLLSARARF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRY2</accession>
    <name>Q3SRY2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phasin_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1698</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314957.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1698"/>
    <dbReference type="EnsemblBacteria" id="ABA04959">
        <property type="protein sequence ID" value="ABA04959"/>
        <property type="gene ID" value="Nwi_1698"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1698"/>
    <dbReference type="eggNOG" id="COG5490">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140350_0_0_5"/>
    <dbReference type="OMA" id="SAYKPYE"/>
    <dbReference type="OrthoDB" id="1880107at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018968">
        <property type="entry name" value="Phasin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09361">
        <property type="entry name" value="Phasin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phasin_2" evidence="1">
        <location>
            <begin position="5"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="112" mass="12278" checksum="DC1684374929C324" modified="2005-10-11" version="1">MTKIEDIQQQGKEQFDAAVTTASGFQKGVQAIAVAFGDYSRKSFEDGRAFTEKLAGAKSLDQAIEAQTAYARTAYETFVSESQKIGGLYADLAKQAYKPFEDFTAKFVPDAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS11</accession>
    <name>Q3SS11_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_1669</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1669"/>
    <dbReference type="EnsemblBacteria" id="ABA04930">
        <property type="protein sequence ID" value="ABA04930"/>
        <property type="gene ID" value="Nwi_1669"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1669"/>
    <dbReference type="eggNOG" id="COG3164">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007198_1_0_5"/>
    <dbReference type="OMA" id="GTFVPMY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032712">
        <property type="entry name" value="AsmA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025263">
        <property type="entry name" value="DUF3971"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13502">
        <property type="entry name" value="AsmA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13116">
        <property type="entry name" value="DUF3971"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3971" evidence="3">
        <location>
            <begin position="534"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="domain" description="AsmA_2" evidence="4">
        <location>
            <begin position="1076"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1210" mass="128974" checksum="07D58AF1AD3C8CA7" modified="2005-10-11" version="1">MTKSTPTRDPNPHGDHHVSQGEVAGWGEDDDELHSRRVRRLLSRSDSRFHRAGDRFVRFRNWLAGERWIKRLAVAFLVLSVTFAGGFGALWWRLGAGPLNLDMMTPWLVSAIEGNIGHDNTVEIGGTQIERAGRIRIAVRIRDVVVRDRDRVVIASAPKAEVKLSGLALLTGRLRAESLDLVDAELAVRITPDGQVTVSTGDNAKPLATGVAPPLRSGLPSAVVSSETSPPPQATQQLSDSPAAAPASSESSGNAGALLAGLEWLDGLGVRGLDGQNLNEIGLKNGRLVVDDQQRGNRWSFSNISLSLHRPSAGGVAFTVSEGGSKEWSLHVAVGPPANGVRPIELRAAGIPAKTVLLALRIKDLTYSADMPLSGELKGEIGRDGLPTYLRGNLFAGKGTIIDSDTPDYPMAIDQIEMNVEWDSGRRVLLVPFKIVSGENRITLLARLQPPNGNVPDWQMGLSGGTIVLAGSDGEAPLIFNRIAVGLRFDTGHRQAILTQADISNGEIGIAGTGSIDYSDAPRLKLGFAGTPMSAAALKSIWPAVIVPELREWVIGRIKRGFLKRIEIGVNSPLHNLSRRGPPMPDDGLSVDIVADNVTIYPVDELPLVHDAGLKARVTGRTAAVTIEQAVADTPSGRRLNISDVVFEVPDMAPKPSPAKVRFRIDSPVPAAAEILVSDRLNEFTDNLIDPNSSKGTVAAQIALGLPLTRQITKEDTTYSITADLTGFAADRLVMNQKLEASALKVTANNEGYHVKGDVKINGQPASLDYRKPADGDADIKLQATLDDASRAKLGIDLGAGATGSIPVKLAGKIGRNRDNKFAVDADLTSLKLDNILPGWVKLPGKSGHATFNVVQKAQSTRLEDIIVDGSGVTIKGSLEVDQNGDLVNVNFPVYAPSEGDRASLKAERNADGVLKVVMRGDVFDGRGFLKSAISGKQADARSKSKSIDFDIDLKIGAVAGFYGEAIRSADVKMAQRNGSVRSLTMTGKIGRNTPLTGSLRGRPQGRDVVLIETDDAGAFFRFTDTYGKMFGGQLALAMDPPTAEQRAKQGLINVRDFTIKGESALDRAAAGGPRTAQNGISFSRLRAEFSRQNGQLTVRDGVVKGPTIGATIEGRIDYPANEVRMSGTFVPMYGLNNIFGQLPVVGLILGGGSDEGLIGVTYEVVGTPGQPQLRINPISAMAPGLVRKIFEFQTGRTANQIEFPATSGN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-16" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS12</accession>
    <name>SYY_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tyrosine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Tyrosyl-tRNA synthetase</fullName>
            <shortName evidence="1">TyrRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tyrS</name>
        <name type="ordered locus">Nwi_1668</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + H(+) + L-tyrosyl-tRNA(Tyr)</text>
            <dbReference type="Rhea" id="RHEA:10220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9706"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78536"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.1" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314928.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SS12"/>
    <dbReference type="SMR" id="Q3SS12"/>
    <dbReference type="STRING" id="323098.Nwi_1668"/>
    <dbReference type="PRIDE" id="Q3SS12"/>
    <dbReference type="EnsemblBacteria" id="ABA04929">
        <property type="protein sequence ID" value="ABA04929"/>
        <property type="gene ID" value="Nwi_1668"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1668"/>
    <dbReference type="eggNOG" id="COG0162">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024003_0_3_5"/>
    <dbReference type="OMA" id="YMMAKDS"/>
    <dbReference type="OrthoDB" id="402899at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004831">
        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006437">
        <property type="term" value="P:tyrosyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00805">
        <property type="entry name" value="TyrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02006">
        <property type="entry name" value="Tyr_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002307">
        <property type="entry name" value="Tyr-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024088">
        <property type="entry name" value="Tyr-tRNA-ligase_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024107">
        <property type="entry name" value="Tyr-tRNA-ligase_bac_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11766">
        <property type="entry name" value="PTHR11766"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01040">
        <property type="entry name" value="TRNASYNTHTYR"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00234">
        <property type="entry name" value="tyrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <feature type="chain" id="PRO_0000234742" description="Tyrosine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="S4 RNA-binding" evidence="1">
        <location>
            <begin position="350"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="236"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02006"/>
        </source>
    </evidence>
    <sequence length="417" mass="46100" checksum="F29017F21A6020F3" modified="2005-10-11" version="1">MVAFKSDFLNTLQERGFIHQCSDFEGLDALASRNQVTAYTGYDCTAPSLHVGHLLSIMMMHWLQQTGNKPIALMGGGTTRVGDPSGRDETRKILSYDQIDANKESIKGTFLKFLVFGDGKNDAIMTDNAEWLTRLNYIEMLRDVGRHFSINRMLTMDSVKMRLEREQELSFIEFNYMILQSYDYVELARRHGCNLQMGGSDQWGNIVNGVDLGRRMGTHQLYALTCPLLTTSSGAKMGKTAAGAVWLNATMLPVYDYWQYWRNVEDADVGRFLKLFTILPMGEIAKLAALQGSEINEAKKVLATEATALLHGRDEAEKAADTARTTFEQGAISESLPTLDIPRSELASGAGVLALFVKAGLVASNGEARRQIKGGGLRVNDVAVADDKMVLTPDHLTPEGVIKLSMGKKRHVLLRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS40</accession>
    <name>Q3SS40_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1640</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314900.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1640"/>
    <dbReference type="EnsemblBacteria" id="ABA04901">
        <property type="protein sequence ID" value="ABA04901"/>
        <property type="gene ID" value="Nwi_1640"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1640"/>
    <dbReference type="HOGENOM" id="CLU_177031_0_0_5"/>
    <dbReference type="OMA" id="HRQPRRD"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229241" evidence="2">
        <location>
            <begin position="22"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="86" mass="9140" checksum="2580876B399FC0D9" modified="2005-10-11" version="1">MSRISVIAVSAVLIGAPAALAQYPSGSGSSPGLTIDRFSRDSTPIGHRQPRPSDLPPDSRDARDRDRLDPADAALQRKIKSICRGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS99</accession>
    <name>Q3SS99_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1581</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314848.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1581"/>
    <dbReference type="EnsemblBacteria" id="ABA04842">
        <property type="protein sequence ID" value="ABA04842"/>
        <property type="gene ID" value="Nwi_1581"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1581"/>
    <dbReference type="eggNOG" id="ENOG5032UBW">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133201_0_0_5"/>
    <dbReference type="OMA" id="YGIRRVR"/>
    <dbReference type="OrthoDB" id="1955141at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="99" mass="11045" checksum="F42BC2D0DE1741A2" modified="2005-10-11" version="1">MSEGWHTKYGIRRVRRDPPTLEEAIFAATGITDDLDSQAEIAASLIGMPFDIVHAEVKKTARAVTRSPTTRVIVGEQGALRSVVVERRVVRRFGNDKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSB5</accession>
    <name>Q3SSB5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phage integrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1565</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1565"/>
    <dbReference type="EnsemblBacteria" id="ABA04826">
        <property type="protein sequence ID" value="ABA04826"/>
        <property type="gene ID" value="Nwi_1565"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1565"/>
    <dbReference type="eggNOG" id="COG0582">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027562_11_0_5"/>
    <dbReference type="OMA" id="IREIGHA"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyr recombinase" evidence="3">
        <location>
            <begin position="175"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="390"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="416" mass="47343" checksum="3930DABA48F708CF" modified="2005-10-11" version="1">MSVYPIKGSRYWQFDFQISGYRFSGSTKKTCERDAKAVETAEKAKARELVKSITVAAGEPIRLGAACDRWWAEVGSRGSERDMQHALGRIRDIIGDRTFLHDITDDTLSRLIAERRQDRVRAGRDDNGVQLWRPISNKRVNKTTTGLLRRVINRAVDNWNAVVPRAPKWEKHWLKETKRPIREIGHAEEAAIDAEEDADYAALRRFAVITGLRRGSVLLRWPQVDFEQAVVRVITKGGVPRVIPLTKEAYALLWARRGHHPEAVFTFIARRTNKNAKNGQQYIRGQRYPITVYGFVSHWRYATKRAGVKARIHDMRHTAATRTLRETGNLRLVQKLLGHTDIKITAEFYADVLVEDLREGMERTSAAERQAVAKLESGRAVERECAVEVAGNPCNKSRGTSATTDKPLRSRRNSEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSC2</accession>
    <name>Q3SSC2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1558</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04819">
        <property type="protein sequence ID" value="ABA04819"/>
        <property type="gene ID" value="Nwi_1558"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1558"/>
    <dbReference type="HOGENOM" id="CLU_2437840_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="90" mass="10064" checksum="B10DE207B4B1832F" modified="2005-10-11" version="1">MSDDERKWPYGLATDHIDRLHDLDLHPPEVFSDAAQIFESGGILRFVFRASRPELGDPYSDPTVPVARVAISRRYFTAALQDALAKLPPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSD9</accession>
    <name>Q3SSD9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Helix-turn-helix, Fis-type</fullName>
            <ecNumber evidence="3">2.7.7.49</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1541</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04802">
        <property type="protein sequence ID" value="ABA04802"/>
        <property type="gene ID" value="Nwi_1541"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1541"/>
    <dbReference type="eggNOG" id="COG2801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2963">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_569646_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010921">
        <property type="entry name" value="Trp_repressor/repl_initiator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48295">
        <property type="entry name" value="SSF48295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="301"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="495" mass="56603" checksum="FDE9D0F725237123" modified="2005-10-11" version="1">MQGRDIRRRYFSKNFRVCPPSGLLRQFRSFREGRISGSSQPIWSADETEIRTRQSAGRTGAEGHPTADAPALFGGGEDPYRAGRTARRGEHLRALPPRRHRRLDVLRLVQGVPGSRQAPVGGRHGTCRNLWRGERSSSGSLGVEGSRRRSHPGEPSAKKKHERGWGERGMRYPASEKAEIIALVEQSHLPAKRTLDKLGIPRATFYRWYDRYRAGGIEALADHRSRPDRVWNRIPDDVRGQIIDLALELPELSPRELAVRFTDERKYFVSEASVYRLLKAHDLITSPAYVVIKAANEFKDKTTAANQLWQTDFTYLKITGWGWYYLSTVLDDFSRYIVAWRLGPTMCASDVTATLDQALAASGLDHVSVRQRPRLLSDNGSSYVADDLATWLRAKDMQHVRGAPYHPQTQGKIERWHQTLKNRILLENYYLPDDLKRQVAAFVEHYNHDRYHESIGNVTPADVYFGRAETILAERHRIKRDTIANRRLQHQLQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSE5</accession>
    <name>Q3SSE5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Extracellular alpha-helical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_1535</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family. Bacterial alpha-2-macroglobulin subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314802.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1535"/>
    <dbReference type="EnsemblBacteria" id="ABA04796">
        <property type="protein sequence ID" value="ABA04796"/>
        <property type="gene ID" value="Nwi_1535"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1535"/>
    <dbReference type="eggNOG" id="COG2373">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000965_1_0_5"/>
    <dbReference type="OMA" id="LDRYPYG"/>
    <dbReference type="OrthoDB" id="231654at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004866">
        <property type="term" value="F:endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026284">
        <property type="entry name" value="A2-macglob_dom_prot_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011625">
        <property type="entry name" value="A2M_N_BRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011626">
        <property type="entry name" value="Alpha-macroglobulin_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021868">
        <property type="entry name" value="Alpha_2_Macroglob_MG3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041203">
        <property type="entry name" value="Bact_A2M_MG5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041462">
        <property type="entry name" value="Bact_A2M_MG6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041246">
        <property type="entry name" value="Bact_MG10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001599">
        <property type="entry name" value="Macroglobln_a2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002890">
        <property type="entry name" value="MG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00207">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07703">
        <property type="entry name" value="A2M_BRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17973">
        <property type="entry name" value="bMG10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11974">
        <property type="entry name" value="bMG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17972">
        <property type="entry name" value="bMG5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17962">
        <property type="entry name" value="bMG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01835">
        <property type="entry name" value="MG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07678">
        <property type="entry name" value="TED_complement"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038980">
        <property type="entry name" value="A2M_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01360">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01359">
        <property type="entry name" value="A2M_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="SSF48239"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229194" evidence="3">
        <location>
            <begin position="25"/>
            <end position="1739"/>
        </location>
    </feature>
    <feature type="domain" description="A2M_N_2" evidence="4">
        <location>
            <begin position="872"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="domain" description="A2M" evidence="5">
        <location>
            <begin position="1077"/>
            <end position="1166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA04796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1739" mass="186472" checksum="A77D7962E8C45E96" modified="2005-10-11" version="1">MTRLVRAVAFCATLLIGLGSVAHAADKAFKRDDLADAAIKLEARIKSEAGAVAKSGSMLRTDADTAFKRADFRSGLQILGQIATVAPNDSGNWLRLARTIFQIKPATSQEQTFLLERASTAAYLAYQRATDPGTEADALAVLGRAFSDRKLWRPALDALRLSLDLREVASVREQYEKMRDDHGFRLLDYTVDSDSATPRVCFQFSEDLAKRVDFSPFVALAGDDRPALSSEDRQLCVEGLKHGERYNINLRAGLPSAVKESLPKSAEFNVYVRDRKPFVRFTGRAYVLPRTGQRGIPLVSVNTQNVTVKVFRIGDRNLINTVVESDFQKALGSHQLYELGHERGIKVWSGEVTTASTLNADVTTAFPVDEALGNLQPGVYVMTAAPKGPGSTDDDESGSLATQWFIVSDLGLSAYSGNDGIHVFVNSLATTDAVDKAEVRLVARNNEILATRKTDASGHALFEPGLARGEGGQSPALLTVSTDKADYAFLSLKSNAFDLTDRGVSGRAVPSGADAFVYAERGVYRSGETVYLTALLRDGQGVAVTGGPLTLVVERPDGVEYRRAVLSDHGSGGRSLDLPLNSAVPTGTWRVRAFTDPKGPSVGETTFMVEDYVPDRIEFDLTTKAKQIAADNPVELKVDGRFLYGAPASGLQLEGELLVAPAESRPGYAGYRFGVPDDEAASNERTPIENLPEADANGVATFPVSLATAPSSDRQHEAQIFIRMAEAGGRAVERKIVLPVKPSAAMIGVKPLFADKSVAEGDRARFDVVFVAPDGTSLARKGLRYELLKLESRYQWYRQNSYWEYEPVKSTRRVADGDLSIAADSPARIELSPQPGRYRLDVKSSDSDGPLTSVQFDVGWYSDGSADTPDLLETSIDKPDYQSGDTMVVSVNARTAGKLTINVLGDRLLTTQTTEVKEGTSQVKIPVGKDWGTGAYVVATLRRPLDVAAQRMPGRAIGIKWFGIDRSARTLSVNLSPPELARPSAPLKLPVKVGGLSPGEDAKIVVAAVDVGILNLTNYKPPAPDDYYLGQRRLTSEIRDLYGQLIDGMQGTRGQLRTGGDFAGAELQGSPPTQKPLALYSGIVTVAADGTAEISFDIPEFAGTARVMAVAWTATKLGRATVDVTVRDPVVLTATLPRFLLTGDRGTMSFDLDNVEGPPGDYTVNVRTSGPVKVAGNATTAIKLAAGQRSSMALTLDTAGSAGAARFDIDIKGPNGLRLARHYDLEVKPATQILARRSVRTLAKGESLTLTSDMFSDLVAGTGGVSMSVGLSSALDAASVLKALDRYPFGCSEQIASRAMPLLYVNDLAAEAHLAMDTSIDDRIKSSIERLLARQGSNGSFGLWSSGGEDSWLDAYVTDFLTRAREKGFSVPDIQFTSALDRIRNSVVNAAEPEKDGGRDLAYGLYVLARNGAAPIGDLRYLADTKLNNLATPIAKAQLAAALALVGDRARAERVYIAAAESLAPKPALEFGRADYGSALRDAAALVSLASEGNAPRATVTQAVQRVEAARGLTSSTSTQENAWLVLAARALAKESMSLDVNGAPVKAALYRSHKAAELADKPIKITNTGEAPVQAVISVAGAPVTPEPATSNGFVIERNYFNLDGTPADPTQATQNDRLAVVLRITETKPEYGHILVADYLPAGFEIDNPNLVSSGDTGTLDWIEDGKEPVSTEFRDDRFTAAIDRTASDKAVFTVAYVVRAVSPGKYVLPQAYVEDMYNPSRYGRTSTGRVEVRSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSF1</accession>
    <name>Q3SSF1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Amino acid ABC transporter substrate-binding protein, PAAT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_1529</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the bacterial solute-binding protein 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314796.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1529"/>
    <dbReference type="EnsemblBacteria" id="ABA04790">
        <property type="protein sequence ID" value="ABA04790"/>
        <property type="gene ID" value="Nwi_1529"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1529"/>
    <dbReference type="eggNOG" id="COG0834">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019602_3_2_5"/>
    <dbReference type="OMA" id="RNTTWTF"/>
    <dbReference type="OrthoDB" id="685252at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018313">
        <property type="entry name" value="SBP_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00497">
        <property type="entry name" value="SBP_bac_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01039">
        <property type="entry name" value="SBP_BACTERIAL_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229248" evidence="4">
        <location>
            <begin position="23"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="PBPb" evidence="5">
        <location>
            <begin position="42"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA04790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="346" mass="37753" checksum="E927CA11F9713233" modified="2005-10-11" version="1">MKCVFTAIFAIVAGISLQPASAQEQESAKAKTTLEAVKARGALSCGVSQGLPGFSAPDDKGNWTGLDVDICRAIAAVVFNDPTKVKFVPLSAKDRFIALQSGEIDVLSRDTTWTLSRDTSLGANFTGVTYYDGQGFLIRKSLKVNSALELNSASVCVQTGTTNEQNLADYFKSNKMKYEVIAFSTADETIKAYESGRCDVFTSDISQLYAQRLKLINPADHAVLPEVISKEPHATMVRHGDDQWFDLVKWTLFAMINAEELGITKKNVDEMARSENPELKRIFGTDGNFGEQLGVTKDWVSRIIKAVGNYGESFDRNVGAGSPLGIARGLNKLWNQGGIMYAPPIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSF9</accession>
    <name>Q3SSF9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acriflavin resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1521</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314788.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1521"/>
    <dbReference type="EnsemblBacteria" id="ABA04782">
        <property type="protein sequence ID" value="ABA04782"/>
        <property type="gene ID" value="Nwi_1521"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1521"/>
    <dbReference type="eggNOG" id="COG0841">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002755_1_2_5"/>
    <dbReference type="OMA" id="QACLMRF"/>
    <dbReference type="OrthoDB" id="145346at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2090.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027463">
        <property type="entry name" value="AcrB_DN_DC_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001036">
        <property type="entry name" value="Acrflvin-R"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32063">
        <property type="entry name" value="PTHR32063"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00873">
        <property type="entry name" value="ACR_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00702">
        <property type="entry name" value="ACRIFLAVINRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82714">
        <property type="entry name" value="SSF82714"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="337"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="361"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="432"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="464"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="525"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="861"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="898"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="961"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="991"/>
            <end position="1015"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1032" mass="110863" checksum="8DBC3F036C76F438" modified="2005-10-11" version="1">MASISEPFIRRPVGTTLLAIGLFLIGVVAYGVLPVASVPNVDFPMIRVSASRPGASPSVMAATVAAPLERRLGEIAGVEQITSSSTLGSTSIHLQFAIGRDIDHAARDVQAAINASLANLPTDMPTLPKFRKANPAASPVFILALTSKTMSTSAIYDVADTVLVQRVSQVPGVGEVSVSGADQPAVRIALNPVALSNAGISTDDVRTAIVNANPLGPVGIFNGDRQSETLSTNRQMRSAAEFRDIVIKSSGGNFVRLSDVAEVKDATRNSRSIAWFNKQPAVLIQVTKQGDANVIDTVDRVRALLPELKQWIPAGIEVSIIVDRTGTIRASVEDMEWTLLATALLVMVVVFAFLRRFTPTVAAGVSVPLALAGTCAGMWLAGFSIDNLSLMALAISVGFVVDDAIVMIENMYRNLEQGLPPYRAALEGARQIGFTVLSISLSLIAAFTPLIFMDGIVGRLLREFSLTLTFAIVVSTIVSLTVTPMICAHYIKRATSTDATRFDRLVEGLLSRIVAFYTRTLRVVLGFPVLMLLVFFATIALTVTLYIKTPKGYFPIDDSGFIIGATRASPDVSFQSMLELQQRVMDIVMADPAVEGIGSSIGGGGMRAGSNRGFMYISLKPPAERGKLTTQQAIDRLRRSLGDVPGIRLFMFAAQDIGRGGRQSDSNYQYTLISTDLDLLQKWAPLVAKRMQAIEGVTDVSSDREAGGLQLTLSIDRQRASSLGVRVQDIDNALNNAFSQRQISIVYTQRNQYQVILEIDPRFQEDPSHLERIFVAGANGVQIPLSAVARYERDLSPLAVYHSQSFPSVTVSFNTLPGVELQDATANIQRAVDELHMPEGIRGSFEGSAADFRKTSGRQPLLILGALVAVYIVLGVLYESLAHPLTIISTLPSAGLGALLALQITGTPLTIIAFVGIILLIGIVKKNGIMIVDFALEAERQRGLSSADAIFEACRVRFRPIIMTTMAALFAAIPLVLATGPGTELRRPLGITIIGGLLVSQILTLYTTPVIYLLIDRIRRRGEAIPAAAPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSI0</accession>
    <name>Q3SSI0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cupin region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1500</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314767.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1500"/>
    <dbReference type="EnsemblBacteria" id="ABA04761">
        <property type="protein sequence ID" value="ABA04761"/>
        <property type="gene ID" value="Nwi_1500"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1500"/>
    <dbReference type="eggNOG" id="COG1917">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072993_1_4_5"/>
    <dbReference type="OMA" id="YGPAENF"/>
    <dbReference type="OrthoDB" id="1792151at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin_2" evidence="1">
        <location>
            <begin position="43"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="136" mass="14314" checksum="0B947A729F96581B" modified="2005-10-11" version="1">MDIHQAGSRPAKPGPGEYFSGTVSVEPIIASVNAPSRVLAASVAFEPGARTAWHTHPLGQTLYVVSGVGLVQTEGGPIREIHPGDTVWFSPGEKHWHGAAPTSAMTHIAVHEALNGSAVTWMEHVTDEEYAGKISG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSJ3</accession>
    <name>Q3SSJ3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phage integrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1487</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314755.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04748">
        <property type="protein sequence ID" value="ABA04748"/>
        <property type="gene ID" value="Nwi_1487"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1487"/>
    <dbReference type="eggNOG" id="COG0582">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027562_17_1_5"/>
    <dbReference type="OMA" id="DVAIPPH"/>
    <dbReference type="OrthoDB" id="785253at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyr recombinase" evidence="3">
        <location>
            <begin position="169"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="376" mass="42028" checksum="7260264C2F06EC5D" modified="2005-10-11" version="1">MKGHIQQRGKTSWRLKFDAGRDEKTGKRNVQFVTFRGTKRQAQTKLAELIASVAQSKYIEPSKITVADYVRGRVDQWEDSGDISARTAARYRELTENQIVPHLGAKLIQKLRTDDIEEWHTTLRSGGRAKGKGGLAPRTIGHAHRVLGKAMRDALRFEKITKNVTALQAAPKVDDDEMAIVQDVPALIDRLRHHRLFPLAMIALFTGMRIGEVLALRWGRVDLDRKVIEVREAIEETKAHGIRIKPPKTKAGRRDVTLPDVLVDVLRDYRKTHLERRVALGLGKLPDDALLFAGPGGSLPSQKYYSKAWSDLDVGVPFHGLRHTHASQLIDAGVDIVTISKRLGHAKPDITLRIYAHLFRKDDSKAAAAINAAFNG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSL6</accession>
    <name>KTHY_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thymidylate kinase</fullName>
            <ecNumber evidence="1">2.7.4.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">dTMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tmk</name>
        <name type="ordered locus">Nwi_1464</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + dTMP = ADP + dTDP</text>
            <dbReference type="Rhea" id="RHEA:13517"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58369"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thymidylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.9" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314732.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSL6"/>
    <dbReference type="SMR" id="Q3SSL6"/>
    <dbReference type="STRING" id="323098.Nwi_1464"/>
    <dbReference type="EnsemblBacteria" id="ABA04725">
        <property type="protein sequence ID" value="ABA04725"/>
        <property type="gene ID" value="Nwi_1464"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1464"/>
    <dbReference type="eggNOG" id="COG0125">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049131_0_0_5"/>
    <dbReference type="OMA" id="VMTREPG"/>
    <dbReference type="OrthoDB" id="1585072at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004798">
        <property type="term" value="F:thymidylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006233">
        <property type="term" value="P:dTDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00165">
        <property type="entry name" value="Thymidylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039430">
        <property type="entry name" value="Thymidylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018095">
        <property type="entry name" value="Thymidylate_kin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018094">
        <property type="entry name" value="Thymidylate_kinase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02223">
        <property type="entry name" value="Thymidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00041">
        <property type="entry name" value="DTMP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01331">
        <property type="entry name" value="THYMIDYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000023237" description="Thymidylate kinase">
        <location>
            <begin position="1"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="20"/>
            <end position="27"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00165"/>
        </source>
    </evidence>
    <sequence length="230" mass="24575" checksum="FF681EF1C3A9CE71" modified="2005-10-11" version="1">MAQATPKADSQRGRFITFEGGEGSGKSTQIRILAERLQAAKLRAIVTREPGGSPGAEIIRHLVLSGVGKLLGADAETLLFAAARDDHVHTVIKPALEQGIWVLCDRFADSTRVYQGQLGNVLPGVLNAMERVTIGDLKPDLTVILDVPVEVGLQRAAARRGSGAPDRFEAEDIAFHRKLRDAYRAIAASDPQRCVVIDADADLDAVAARVWAALHDRALTADAATKASRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSL7</accession>
    <name>Q3SSL7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DNA polymerase III, delta prime subunit</fullName>
            <ecNumber evidence="1">2.7.7.7</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1463</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.7" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314731.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1463"/>
    <dbReference type="EnsemblBacteria" id="ABA04724">
        <property type="protein sequence ID" value="ABA04724"/>
        <property type="gene ID" value="Nwi_1463"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1463"/>
    <dbReference type="eggNOG" id="COG0470">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006229_4_4_5"/>
    <dbReference type="OMA" id="GGWRVCI"/>
    <dbReference type="OrthoDB" id="1971388at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="346" mass="37170" checksum="F0E7E9F88CDA0B1D" modified="2005-10-11" version="1">MIARPAATETPFPHPRETTALFGHGEAERTLLDAYRSGRIPHAWLIGGAPGIGKATLAYRMARFVLAHADPATPRVQSAETLELDLMHPAIRHVAADTHGGLLTLERTLNEKGVMRTVISVEQTRETIAFFGSTATGNGWRVCIVDPVDELNPNAANALLKVLEEPPHRSLFFLISHAPARVLATMQSRCRKLMLRPLAASDVISAAAAAAGIAPDDASLRNVAGAAEGSVARALALLGGGALKLHQRTTALLNNLPHIDPGELHALGDAISGSDRAALGAFIDSVDRWMGERLRISDANADLPRLARLAEVWEKINNTARETETYNLERKPLVFSVFGLLAEATR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSQ8</accession>
    <name>RS9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsI</name>
        <name type="ordered locus">Nwi_1422</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314692.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSQ8"/>
    <dbReference type="SMR" id="Q3SSQ8"/>
    <dbReference type="STRING" id="323098.Nwi_1422"/>
    <dbReference type="EnsemblBacteria" id="ABA04683">
        <property type="protein sequence ID" value="ABA04683"/>
        <property type="gene ID" value="Nwi_1422"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1422"/>
    <dbReference type="eggNOG" id="COG0103">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046483_2_0_5"/>
    <dbReference type="OMA" id="QWKINGR"/>
    <dbReference type="OrthoDB" id="1766414at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00532_B">
        <property type="entry name" value="Ribosomal_S9_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_S9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023035">
        <property type="entry name" value="Ribosomal_S9_bac/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020574">
        <property type="entry name" value="Ribosomal_S9_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="PTHR21569"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00360">
        <property type="entry name" value="RIBOSOMAL_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_1000051271" description="30S ribosomal protein S9">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00532"/>
        </source>
    </evidence>
    <sequence length="158" mass="17309" checksum="008F882683874E7B" modified="2005-10-11" version="1">MADTIQSLDQLSALKPAESEAPKHTKKVDKYGRAYATGKRKDAVARVWIKPGAGKILVNAREVDVYFARPVLRMMIQQPLVAAARAGQYDVVCTVAGGGLSGQAGAVRHGLSKALTHFEPELRGVLKKGGFLTRDSRVVERKKYGKAKARRSFQFSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSR4</accession>
    <name>Q3SSR4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acetyltransf_6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1416</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1416"/>
    <dbReference type="EnsemblBacteria" id="ABA04677">
        <property type="protein sequence ID" value="ABA04677"/>
        <property type="gene ID" value="Nwi_1416"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1416"/>
    <dbReference type="eggNOG" id="COG5653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046277_2_0_5"/>
    <dbReference type="OMA" id="MMFNTYT"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038740">
        <property type="entry name" value="BioF2-like_GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13480">
        <property type="entry name" value="Acetyltransf_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyltransf_6" evidence="1">
        <location>
            <begin position="190"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="395" mass="44061" checksum="EB4DDE5BDD8D80EE" modified="2005-10-11" version="1">MTALIGSPTVKAQERSDGRRIAHLEILQDLREAEPIWRNLEVSQFSTPYQRFDLLSAWQRHVGTGEQLQPGIIVAFDAQRQPLLLLPLAIGTESGVRVARFMGGKHTTFNMALWQREFAASATRGDLDALVQGISALPARIDVLAFTQQPRRWRDQQNPMALLPNQPSANGCPLMILSPASSPSERISNSFRRRLKGKERKLQALPGYRYCVATTDTEIKRMCDAFFAIKPLRMAAQKLPNVFAEAGVEDFLRSACLTPLTGGGRVIEIHGLECDAEVIAIFAGVADGHRFSMMFNTYTMSESARYSPGLILMRDIIDHYAENGYTSLDLGIGSDDYKRLFCKSDEPIFDSYLPLTTRGRMAAIGMSSLARAKRLVKQTPALKRTAQMLRGMLHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SST6</accession>
    <name>Q3SST6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ribosomal large subunit pseudouridine synthase C</fullName>
            <ecNumber evidence="5">5.4.99.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_1394</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase RluA family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314666.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1394"/>
    <dbReference type="EnsemblBacteria" id="ABA04655">
        <property type="protein sequence ID" value="ABA04655"/>
        <property type="gene ID" value="Nwi_1394"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1394"/>
    <dbReference type="eggNOG" id="COG0564">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016902_1_2_5"/>
    <dbReference type="OMA" id="RMRIAKH"/>
    <dbReference type="OrthoDB" id="1714699at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120159">
        <property type="term" value="F:rRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd02869">
        <property type="entry name" value="PseudoU_synth_RluA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006225">
        <property type="entry name" value="PsdUridine_synth_RluC/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006224">
        <property type="entry name" value="PsdUridine_synth_RluC/D_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00005">
        <property type="entry name" value="rluA_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01129">
        <property type="entry name" value="PSI_RLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S4" evidence="4">
        <location>
            <begin position="98"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="34"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR606225-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="412" mass="45339" checksum="E85A6C0206BB65F1" modified="2005-10-11" version="1">MSRRDKRSSAESGSRGSERHTNAVAKAARPGKAARSKGRGEVERSAGERWQGVRPGRPQRAGAPRNSVHSAEKPPEPVPAPLPTKVQTVAVTADEANMRVDRFLEARFPGLSFSHIQRVVRKGELRVNGRRADSKDRVSEGDKVRIPPLKLDTPKAAVGLSDAETKIRDELRKMVLFEDADVMVLNKPAGLAVQGGSGLSRHIDGMLETMRDAKGQRPRLVHRLDRETAGCLLIAKTRFAATALTGAFRHRSARKIYWALVAGVPKPKQGRISTYLAKEENEEESIMRIAAHGDEGASHAVTYYAVVETSAQKLAWVSLKPVTGRTHQLRAHMAHIGHAIIGDPKYFNKENWQLPGGLQNRLHLLARRIVIPHPRGGVIDASAPLPPHMLQSWNLLGLEADRFDPIENAPEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST49</accession>
    <name>Q3ST49_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1281</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1281"/>
    <dbReference type="EnsemblBacteria" id="ABA04542">
        <property type="protein sequence ID" value="ABA04542"/>
        <property type="gene ID" value="Nwi_1281"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1281"/>
    <dbReference type="HOGENOM" id="CLU_1833077_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="140" mass="15813" checksum="064997D88A25B598" modified="2005-10-11" version="1">MRRFIRANRSSILGSSPRTCFARKRETPNSLPSLAMREGSEMNDRKPDAGDVGNSTSTAPVSSNRRDTDFHEGFPSSRIAPTGVARPGPENSPRQGVLDRRVFSGFPRTRRGSEENAATKIRERAPMLPWKLWKLEHFRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STB4</accession>
    <name>Q3STB4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Iron-sulfur cluster carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_1215</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds and transfers iron-sulfur (Fe-S) clusters to target apoproteins. Can hydrolyze ATP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the Mrp/NBP35 ATP-binding proteins family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the Mrp/NBP35 ATP-binding proteins family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the MIP18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314506.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1215"/>
    <dbReference type="EnsemblBacteria" id="ABA04477">
        <property type="protein sequence ID" value="ABA04477"/>
        <property type="gene ID" value="Nwi_1215"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1215"/>
    <dbReference type="eggNOG" id="COG0489">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024839_0_0_5"/>
    <dbReference type="OMA" id="SIPLMMN"/>
    <dbReference type="OrthoDB" id="1413173at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000808">
        <property type="entry name" value="Mrp_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044304">
        <property type="entry name" value="NUBPL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42961">
        <property type="entry name" value="PTHR42961"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="SSF117916"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01215">
        <property type="entry name" value="MRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FeS_assembly_P" evidence="7">
        <location>
            <begin position="6"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="83"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="99"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="134"/>
            <end position="141"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA04477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="379" mass="40079" checksum="143FE555142A20AA" modified="2005-10-11" version="1">MNVTPQQVLDRLAGVLSPSGVALTAAGVLSEIAINGDKVFFSISVEAEESKSWEEVRVNAQAAVRAIPGVSAVMVALTAERKPDRATAGLPPGSEQGIPHVSTRRSPQHPASGSPMARQADIPGVAAVVAVASGKGGVGKSTTALNLALGLRDSGLRVGLLDADIYGPSIPRLTGIREKPRLTDDKKIVPIERFGLAIMSVGFLIEEESAVIWRGPMVTSAIRQMLRDVVWGDLDVLVVDMPPGTGDAQLTLAQNVPLKGAVIVSTPQDLSLIDARRGLAMFRKVNVPVFGIIENMSFFQCPHCGGRSDIFGHGGARHEAERLGVPFLGEVPLHMSIRETSDSGDPVVESEPDGPHAAIYRAMAASIRNRLQDATPKVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STC6</accession>
    <name>Q3STC6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Histone-like DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1203</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial histone-like protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314494.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1203"/>
    <dbReference type="EnsemblBacteria" id="ABA04465">
        <property type="protein sequence ID" value="ABA04465"/>
        <property type="gene ID" value="Nwi_1203"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1203"/>
    <dbReference type="eggNOG" id="COG0776">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105066_0_2_5"/>
    <dbReference type="OMA" id="TGEPMTF"/>
    <dbReference type="OrthoDB" id="1847472at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000119">
        <property type="entry name" value="Hist_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010992">
        <property type="entry name" value="IHF-like_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33175">
        <property type="entry name" value="PTHR33175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00216">
        <property type="entry name" value="Bac_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00411">
        <property type="entry name" value="BHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47729">
        <property type="entry name" value="SSF47729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="99" mass="10660" checksum="762B1E9B946B1FED" modified="2005-10-11" version="1">MAAQMTKSQLVEKISTQVGLSKKDVKGVLEALATTGYKELKKTGAFLLPGFAKFVVIKKPATKARKGTNPFTGEPMTFKAKPARKIVRARPVKAAKDAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STC7</accession>
    <name>Q3STC7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1202</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314493.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1202"/>
    <dbReference type="EnsemblBacteria" id="ABA04464">
        <property type="protein sequence ID" value="ABA04464"/>
        <property type="gene ID" value="Nwi_1202"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1202"/>
    <dbReference type="eggNOG" id="ENOG50339FW">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130407_2_0_5"/>
    <dbReference type="OMA" id="LELMQFW"/>
    <dbReference type="OrthoDB" id="1879824at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017015">
        <property type="entry name" value="UCP033367_VanZ"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF033367">
        <property type="entry name" value="UCP033367_VanZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="118" mass="12555" checksum="2D36321450E73170" modified="2005-10-11" version="1">MKILFRLGTAALIAAVVFATLGPARYRPHSPLGQDGEHALAFVLVGLAVGLSFPQRRLRVAAITVVLIGLLEIMQRWAPGRHARLEDFLVDAGAACLGLVGAAAIGWLTARLHGSRPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STD8</accession>
    <name>Q3STD8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cytochrome c-type biogenesis protein cycH / CcmH</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1191</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314482.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1191"/>
    <dbReference type="EnsemblBacteria" id="ABA04453">
        <property type="protein sequence ID" value="ABA04453"/>
        <property type="gene ID" value="Nwi_1191"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1191"/>
    <dbReference type="eggNOG" id="COG4235">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036074_4_1_5"/>
    <dbReference type="OMA" id="IMQQMIG"/>
    <dbReference type="OrthoDB" id="1539126at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017560">
        <property type="entry name" value="Cyt_c_biogenesis_CcmI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03142">
        <property type="entry name" value="cytochro_ccmI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="367" mass="39378" checksum="68EFC12699E87F41" modified="2005-10-11" version="1">MTVWFVFALMTAGAAFAVLWPLGRSGSRRTSGGSETTVYRDQLAEVGRDAAAGLIGEAEAAAARVEIGRRLLEAADAERHQPQDSKLWLRRAVAVVALAGIPLLAFATYLPLGSPQMPDFPLAARIQTADASQSLDSLVARVESHLEKNPGDGRGWTVLAPILAKLGRFDDAARAYRNVITYEGETAPRRADLGAAIAGAANGIVTAEAKKEFERALALNPQEAKARFFLGMAAQQDGRSADAMKIWKALLADAPADAEWRVTVQEALAQLGDGAPRPTEEAVAAASDMNEADRSAMIHTMVDRLAARLKQDGNDVEGWLRLARAYMVLNQPEKSRAVRDDARRALGRNEAGLRQLNEGFKALGIDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STF3</accession>
    <name>Q3STF3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1176</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314467.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1176"/>
    <dbReference type="EnsemblBacteria" id="ABA04438">
        <property type="protein sequence ID" value="ABA04438"/>
        <property type="gene ID" value="Nwi_1176"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1176"/>
    <dbReference type="eggNOG" id="ENOG5032XZR">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_188457_1_0_5"/>
    <dbReference type="OMA" id="QFWRMTP"/>
    <dbReference type="OrthoDB" id="2082692at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011739">
        <property type="entry name" value="GTA_rcc01693"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019056">
        <property type="entry name" value="Phage_TAC_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09550">
        <property type="entry name" value="Phage_TAC_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02216">
        <property type="entry name" value="phage_TIGR02216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="70" mass="7707" checksum="A8C9B52020B62EF7" modified="2005-10-11" version="1">MKPFPWAAAMGFGFGVLRLAPDVFWRMTPRELAQAVHAIRGPATTPLARGELDDLLARFPDAPRKGESDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STG8</accession>
    <name>Q3STG8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phage portal protein, HK97</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1161</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314452.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1161"/>
    <dbReference type="EnsemblBacteria" id="ABA04423">
        <property type="protein sequence ID" value="ABA04423"/>
        <property type="gene ID" value="Nwi_1161"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1161"/>
    <dbReference type="eggNOG" id="COG4695">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054516_0_0_5"/>
    <dbReference type="OMA" id="TYANYRE"/>
    <dbReference type="OrthoDB" id="389520at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006944">
        <property type="entry name" value="Phage/GTA_portal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006427">
        <property type="entry name" value="Portal_HK97"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04860">
        <property type="entry name" value="Phage_portal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01537">
        <property type="entry name" value="portal_HK97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="391" mass="42862" checksum="8CD842BAC82D4404" modified="2005-10-11" version="1">MRLRFKNMFAPPETKASRTARLLAFESGGRARWTPRDYAALAREGYLANPVVHRAVRLIAENVASCSYLVFEGAQEREAHPLSLLLTRPNTRQDGGAFLETLVSHLLLAGNAYVETVALDGAVRELHALRPDRMKVVPGPEGWAEAYEYSVGGRSVRFDQASSVVPPILHLTFFHPLDDHYGLAPIESAAVAIDTHNAGSKWNKALLDNAARPSGALVYAGPEGAVLSDSQFDRLKRELTDTYQGAVNAGRPLLLEGGLDWKAMSLTPKDMDFLEAKHTAAREIALAFGVPPMMLGIPGDNTYANFLEANRCFFRQTVLPLASRIGNSFAQWLSPQFGESIRVVVDTDKMDALAADRTALWERVSDAAFLTLNEKREAVGYAPIEGGDRLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STI9</accession>
    <name>Q3STI9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phage integrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314433.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1140"/>
    <dbReference type="EnsemblBacteria" id="ABA04402">
        <property type="protein sequence ID" value="ABA04402"/>
        <property type="gene ID" value="Nwi_1140"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1140"/>
    <dbReference type="eggNOG" id="COG0582">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_681147_0_0_5"/>
    <dbReference type="OMA" id="HARFAQP"/>
    <dbReference type="OrthoDB" id="1217430at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="405" mass="44959" checksum="4D8E0A0FECEA24A8" modified="2005-10-11" version="1">MEPRETETKARAPGLTWRNGRPVWRATKTAIAAGYPVKNVNLSSYLNQPTLLVQRCERLQAEMLAWVSGRKGRITEFDGTIRSLIEVWQADPESPYHKLKSSTKKPYDVYARMVIMEVGSKLIDDVDGRDVKRWFNAWAKPKKSGAPLQIPKARTAIAVIKSALSFGIMCRKPGCAAFKAVLAEVDFPVPASRTTAPTADDTTKVRQAARAAGHGAAALAYAIQFDGAVRQWDVIGEWVPMSDPRPSIVLDRGMKWIGPMWSQVDENQVFRFTPSKTENTTGKEVAVDFTVCPMIMEELALITSEQRKGPLIVNPVTGKPYRPDYFQKLWRTVRDAQEVSPAIWNRDLRAGGITEGRAADASTDDLAKLAGHANKRTTAKVYDRAQIEAARRVAVARAAHRSKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STK8</accession>
    <name>Q3STK8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Flagellar basal-body rod protein FlgG</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 7">Distal rod protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_1121</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="5 7">The basal body constitutes a major portion of the flagellar organelle and consists of four rings (L,P,S, and M) mounted on a central rod. The rod consists of about 26 subunits of FlgG in the distal portion, and FlgB, FlgC and FlgF are thought to build up the proximal portion of the rod with about 6 subunits each.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Bacterial flagellum basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the flagella basal body rod proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314414.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1121"/>
    <dbReference type="EnsemblBacteria" id="ABA04383">
        <property type="protein sequence ID" value="ABA04383"/>
        <property type="gene ID" value="Nwi_1121"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1121"/>
    <dbReference type="eggNOG" id="COG4786">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013687_0_1_5"/>
    <dbReference type="OMA" id="TRDGTFH"/>
    <dbReference type="OrthoDB" id="1488472at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009426">
        <property type="term" value="C:bacterial-type flagellum basal body, distal rod"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001444">
        <property type="entry name" value="Flag_bb_rod_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019776">
        <property type="entry name" value="Flagellar_basal_body_rod_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020013">
        <property type="entry name" value="Flagellar_FlgE/F/G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010930">
        <property type="entry name" value="Flg_bb/hook_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037925">
        <property type="entry name" value="FlgE/F/G-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012834">
        <property type="entry name" value="FlgG_G_neg"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00460">
        <property type="entry name" value="Flg_bb_rod"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06429">
        <property type="entry name" value="Flg_bbr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117143">
        <property type="entry name" value="SSF117143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03506">
        <property type="entry name" value="FlgEFG_subfam"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02488">
        <property type="entry name" value="flgG_G_neg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00588">
        <property type="entry name" value="FLAGELLA_BB_ROD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flg_bb_rod" evidence="8">
        <location>
            <begin position="4"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="Flg_bbr_C" evidence="9">
        <location>
            <begin position="182"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA04383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="262" mass="27940" checksum="7B4B421D65059827" modified="2005-10-11" version="1">MRALYTAATGMAAQELNVQVISNNIANLRTTGYKKQRAAFQDLIYDHVRRVGAQASTQGTIMPVGIDLGGGVKTVGTPRQMSQGTLSQTGNDLDIAIRGEGFFKIQMPDGTFQYTRDGTFQMDNMGRIVNAQGNPVQPTITIPQNSAGITINAQGQVSVTLPGQTTPTVLGQIGLTRFINKAGLQPVGDNNFKETPASGPFQDGIANLDGFGDMQQSNLEQANVEVVSEISDLIAAQRAYEMNAKVISAADQMLQSAAALFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STN1</accession>
    <name>Q3STN1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FAD dependent oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1098</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1098"/>
    <dbReference type="EnsemblBacteria" id="ABA04360">
        <property type="protein sequence ID" value="ABA04360"/>
        <property type="gene ID" value="Nwi_1098"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1098"/>
    <dbReference type="eggNOG" id="COG0665">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007884_3_0_5"/>
    <dbReference type="OMA" id="GFACFGS"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAO" evidence="1">
        <location>
            <begin position="35"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="457" mass="49448" checksum="FD6DDC3622D62A06" modified="2005-10-11" version="1">MTSDGMAAADGPGDTESAVRARRRERLTFDLDVEICVVGGGLAGLTVALEAARTGANVVVLEGRHVGWNASGHHLGTVMPGYALPLSDLIARVGFDDAYDLWSLARAGADYVRAHAAEHIIPGLGLTEGVLEVSNVDAGERLISHLQMLSEDFSTEVEGWQIDRVRDTLRTGRYFHAVHYPRAFQIDGRKYVHGLAELARKAGARIFEDTPVVGIDPFGIRKRIATPRARLRASHLVLAGNVHLGGALRRLSDTLVPVWRYAGVTSPLGNRLAEVMTFQGSVIDTDGIDHFRIVEGDRLMWASPETTWEARPGRQKAAIGRRIGTIFPQLGAVEIAKVFCGAVGQTVHGMPQIGQLQPGLWVASGFGRQGLGTSAMAGQLIARGMLEGDDRWRLFAPFELVWAGGKTGRAAGHAIGMAMRARSAAAGTLARYRERARIKARERQARLNAADRKSRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STU2</accession>
    <name>Q3STU2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1037</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314333.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04299">
        <property type="protein sequence ID" value="ABA04299"/>
        <property type="gene ID" value="Nwi_1037"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1037"/>
    <dbReference type="HOGENOM" id="CLU_2955935_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="59" mass="6519" checksum="C8A0D63E8714002D" modified="2005-10-11" version="1">MSDATGVNNISGGSLDEEAKKKTGKTMEELTDEKRKELDKKVEQGGDENIKIRTNQILP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STY2</accession>
    <name>Q3STY2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 15">NAD(P) transhydrogenase subunit beta</fullName>
            <ecNumber evidence="6 15">7.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Nicotinamide nucleotide transhydrogenase subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">Nwi_0997</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 15">The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>H(+)(in) + NAD(+) + NADPH = H(+)(out) + NADH + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:47992"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="7.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell inner membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 15">Belongs to the PNT beta subunit family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.1" evidence="6 15"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314298.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0997"/>
    <dbReference type="EnsemblBacteria" id="ABA04259">
        <property type="protein sequence ID" value="ABA04259"/>
        <property type="gene ID" value="Nwi_0997"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0997"/>
    <dbReference type="eggNOG" id="COG1282">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007866_4_0_5"/>
    <dbReference type="OMA" id="KRGMSAG"/>
    <dbReference type="OrthoDB" id="788546at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008750">
        <property type="term" value="F:NAD(P)+ transhydrogenase (AB-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012136">
        <property type="entry name" value="NADH_DH_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034300">
        <property type="entry name" value="PNTB-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02233">
        <property type="entry name" value="PNTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000204">
        <property type="entry name" value="PNTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="8 15" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14 15 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0520">NAD</keyword>
    <keyword evidence="11 15" id="KW-0521">NADP</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 15" id="KW-1278">Translocase</keyword>
    <keyword evidence="10 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="PNTB" evidence="17">
        <location>
            <begin position="8"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00014581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR000204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ABA04259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="465" mass="47991" checksum="0FCF46534B7FEF6D" modified="2005-10-11" version="1">MNADLAALLYLVAGVLFILSLRGLSSPASSRRGNFLGMAGMAIAVATTLAAHPPADAVAWVLVILGVAIGGGIGAVIARRVPMTSMPELVAAFHSLVGMAAVLVAAGAFYAPEAFEIGTPGNIHAQSLIEMSLGVAIGALTFTGSVIAFLKLSGRMSGAPIILPARHIINIALALALVFFMYGLVVSGSALDFWLITILALVIGVLLIIPIGGADMPVVISMLNSYSGWAAAGIGFTLGNSALIITGALVGSSGAILSYIMCHAMNRSFISVILGGFGGETAAAGGAGGEQKPAKLGSADDAAFIMKNASKVIIVPGYGMAVAQAQHALRELADSLKKEGVEVKYAIHPVAGRMPGHMNVLLAEANVPYDEVFELEDINSEFAQADVAFVIGANDVTNPAAEDDKSSPIYGMPVLQVWKAGTVMFIKRSLASGYAGIDNPLFYRDNTMMLLGDAKKMTENIVKGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU80</accession>
    <name>Q3SU80_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ATP-grasp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0897</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0897"/>
    <dbReference type="EnsemblBacteria" id="ABA04161">
        <property type="protein sequence ID" value="ABA04161"/>
        <property type="gene ID" value="Nwi_0897"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0897"/>
    <dbReference type="eggNOG" id="COG0189">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4773">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040640_0_0_5"/>
    <dbReference type="OMA" id="WEFIQFV"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039423">
        <property type="entry name" value="TonB-dep_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32552">
        <property type="entry name" value="PTHR32552"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="2">
        <location>
            <begin position="221"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="513" mass="56627" checksum="9125F2E519F36ABC" modified="2005-10-11" version="1">MQGETAWGPVNGFVASRSATATKTDTPIIEVPQSISVVTRDQIETRRNQTLRRCNTRRAFWHAPRWLHHAVFWLRAFSSHQQDSTWPENALSPVMPTSEARTSPLRILLSEGSSTSAREAITILGMQGHHVEVCDPDPRCFGRFSRFVRRFHRCPGLGVDPAGYVTFVLALLARRPFDVLLPIHEQGYALSKIREAIEKHVAVALPAHAAYQQAHSKASFSRLLSGLDLPQPRTLLVSTSEDVLALDRFPLILKAATGTASRTVWPVRGAHELAAVVRELARCGAFADDIVAQEFIDAPVEHAQAVFDRGRLLGMHAYRQIARGAGGGDAVKESVDRPLVREHLTRIGKHLDWHGALSVDYLTPGANDVLYIDCNPRLVEPMNALLAGHDLLGLLLRVTRGVSPEALVPGRAGVRTHLALQALLGCAMRSRSRLELLRECRRLLMRTGVYRGSQEELTPLRIDWPSVIPTVFAAALLLIRPGAAEHLVSKGWGDHLLNPESIRVIEGWNGPPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUB0</accession>
    <name>Q3SUB0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Type I site-specific deoxyribonuclease</fullName>
            <ecNumber evidence="3">3.1.21.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Nwi_0867</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates, ATP is simultaneously hydrolyzed.</text>
            <dbReference type="EC" id="3.1.21.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HsdR family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.3" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0867"/>
    <dbReference type="EnsemblBacteria" id="ABA04131">
        <property type="protein sequence ID" value="ABA04131"/>
        <property type="gene ID" value="Nwi_0867"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0867"/>
    <dbReference type="eggNOG" id="COG0610">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1203813_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009035">
        <property type="term" value="F:type I site-specific deoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007409">
        <property type="entry name" value="Restrct_endonuc_type1_HsdR_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04313">
        <property type="entry name" value="HSDR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0680">Restriction system</keyword>
    <feature type="domain" description="HSDR_N" evidence="12">
        <location>
            <begin position="115"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="207"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA04131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="230" mass="24926" checksum="A9E37B19BDC0D115" modified="2005-10-11" version="1">MPAIAMNGSPFSMTRYLAFGGLVRLASGERQSNDRFSDRIRFAIRLCWVIYGGQGMHIDHDDLVLKSEGDVEAKLLFPLLMSELYLGIPSQNVFSKEYLAPSDLDKGAKVAGGYYPDASVWVHGFPIVMVEAKAPGVPVEQAYREAALYARHLNSQRPAGFNPANFIIASSGDCPPSGLLRQFRGFREGRISGSSQPIWSADETEIRAGESTGRAGREGHPPGNAPAVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUE6</accession>
    <name>Q3SUE6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, XRE family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0830</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0830"/>
    <dbReference type="EnsemblBacteria" id="ABA04095">
        <property type="protein sequence ID" value="ABA04095"/>
        <property type="gene ID" value="Nwi_0830"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0830"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066192_47_3_5"/>
    <dbReference type="OMA" id="NFMVRVE"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="28"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="88" mass="9336" checksum="585571037DC7CD26" modified="2005-10-11" version="1">MRTDQCLFACSGRMANRTVTPTEIGVIVRTTRKSQKLRQDQLAGAAGVGVRFIVDLEAGKPTAQIGKALLILAALGCKVTIDPPPSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUJ0</accession>
    <name>Q3SUJ0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0786</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314099.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0786"/>
    <dbReference type="EnsemblBacteria" id="ABA04051">
        <property type="protein sequence ID" value="ABA04051"/>
        <property type="gene ID" value="Nwi_0786"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0786"/>
    <dbReference type="eggNOG" id="ENOG50334PS">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_841014_0_0_5"/>
    <dbReference type="OMA" id="VFIANFG"/>
    <dbReference type="OrthoDB" id="1248554at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="326" mass="36729" checksum="B6CC745A3EDD65DF" modified="2005-10-11" version="1">MKVFIANFGRENYEWPVCQTRGTIATMNEVSAQAYWESGDREGYILSRMKGKTAAGITPTKPVASRWFNLMSIIAQSSGDIWIHREKDQFWWTTSRADPPMFEPKVEPVGDKRNVIVCHKPCNPWSDKNRNGNRLDWNGLHPKAREFLFTEGTLQQLGDDNAEYALALIEGADLSSWHSLPAWKAKAEKAREKRGVVTIFNAKQRAAARMAMTARDTVAGANGQQVLRTLKNKELKFASPQELERYLIDLLDLQEGLCAITGLTLQYDGEHEDAEMLCSLDRIDSEGHYEAGNLQVVCRFANRWKGSSSDEGFRRLIGAVRSVGSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUS8</accession>
    <name>Q3SUS8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0697</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314019.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0697"/>
    <dbReference type="EnsemblBacteria" id="ABA03963">
        <property type="protein sequence ID" value="ABA03963"/>
        <property type="gene ID" value="Nwi_0697"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0697"/>
    <dbReference type="eggNOG" id="ENOG502ZB69">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1308150_0_0_5"/>
    <dbReference type="OMA" id="RWDDHRY"/>
    <dbReference type="OrthoDB" id="1289751at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="208" mass="24130" checksum="8EEDD833FE21D45C" modified="2005-10-11" version="1">MMSFREQLRTQRWDDHRYYHHSRINQSLHFVSAFSFLVAYALLFVDPVAAALIGWLFSMTTRQAGHFFFEPKGYDHGNHATHDHKEEIKVGYNLNRKIVLMAIWALSPLVLFLDPTLFGVFTPWASPADFMRQVAKVWLAIGIGGVLFRTVHLFFIRDVETGLVWMTKILTDPFHDLKLYYKAPLYLMKGELIDPGLGQVQTRKARSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUU0</accession>
    <name>Q3SUU0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Aliphatic sulfonate ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0685</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0685"/>
    <dbReference type="EnsemblBacteria" id="ABA03951">
        <property type="protein sequence ID" value="ABA03951"/>
        <property type="gene ID" value="Nwi_0685"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0685"/>
    <dbReference type="eggNOG" id="COG1116">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_192113_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="88" mass="9883" checksum="9E3E602BB944B390" modified="2005-10-11" version="1">MQRLLERVWSDQAFTAILVTHDVSEAVALADRVLVIEQGRIAHDVEINIARPRRRGIARPRRRGSADLAAIEGSILQELLKEDRSTDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVG6</accession>
    <name>Q3SVG6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter, ATPase subunit</fullName>
            <ecNumber evidence="7">3.6.3.25</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0458</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in beta-(1--&gt;2)glucan export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.25" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313789.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0458"/>
    <dbReference type="EnsemblBacteria" id="ABA03725">
        <property type="protein sequence ID" value="ABA03725"/>
        <property type="gene ID" value="Nwi_0458"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0458"/>
    <dbReference type="eggNOG" id="COG1116">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_5"/>
    <dbReference type="OMA" id="QSIVTFP"/>
    <dbReference type="OrthoDB" id="1832232at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="19"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="53"/>
            <end position="60"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="270" mass="29484" checksum="A3EDF8C361B05B97" modified="2005-10-11" version="1">MTALAQTILPAPDRQVAVLEAAGVVLRYPGQLKPVVEAFDFELDAGETVSVLGPSGVGKSSLLRVLGGLQKPTDGTVTMNGKPLRGVPPRVSIAFQDASLLPWLSLEKNVAFGLNFKHQPHLTPEESKVRVDVAVREVGLTHARRYFPAQLSGGMAQRAALARCLARKPEILLLDEPFGALDEVTRTEMQHLLLKVVRDFHTAAILITHDIDEALLVSDRILLLGGTPARVIGQWRIDLPTPREDYIAELGAIRVEILSRLRGATLRRIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVG9</accession>
    <name>Q3SVG9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0455</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313786.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0455"/>
    <dbReference type="EnsemblBacteria" id="ABA03722">
        <property type="protein sequence ID" value="ABA03722"/>
        <property type="gene ID" value="Nwi_0455"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0455"/>
    <dbReference type="eggNOG" id="COG3753">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084747_0_0_5"/>
    <dbReference type="OMA" id="GMQNGPH"/>
    <dbReference type="OrthoDB" id="1727566at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045372">
        <property type="entry name" value="YidB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027405">
        <property type="entry name" value="YidB-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20159">
        <property type="entry name" value="YidB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140804">
        <property type="entry name" value="SSF140804"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="192" mass="19221" checksum="44F1809E1FEDC78D" modified="2005-10-11" version="1">MGLLDVLNGMQNGPRGPSQPGAESESGGMSPLTLAILAVLGYKALKHLTGGQPDPSPAAPPPPAPGRTVNAGLPGSTGGLGDLLKGGLGGLLAGGAAGSVLSGGLGELLNQLQKKGHSETANSWVGPGPNKQIAPRDLADALGADQINALTAQTGLSQDELLTELSKHLPEVVHHLTPDGRLPTESELSRLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVI4</accession>
    <name>RL27_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpmA</name>
        <name type="ordered locus">Nwi_0440</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL27 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313771.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVI4"/>
    <dbReference type="SMR" id="Q3SVI4"/>
    <dbReference type="STRING" id="323098.Nwi_0440"/>
    <dbReference type="EnsemblBacteria" id="ABA03707">
        <property type="protein sequence ID" value="ABA03707"/>
        <property type="gene ID" value="Nwi_0440"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0440"/>
    <dbReference type="eggNOG" id="COG0211">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095424_4_1_5"/>
    <dbReference type="OMA" id="GKDHTLH"/>
    <dbReference type="OrthoDB" id="1904463at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00539">
        <property type="entry name" value="Ribosomal_L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001684">
        <property type="entry name" value="Ribosomal_L27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018261">
        <property type="entry name" value="Ribosomal_L27_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15893">
        <property type="entry name" value="PTHR15893"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01016">
        <property type="entry name" value="Ribosomal_L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00063">
        <property type="entry name" value="RIBOSOMALL27"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00062">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00831">
        <property type="entry name" value="RIBOSOMAL_L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_1000017534" description="50S ribosomal protein L27">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="90" mass="9531" checksum="900B0140ED151A05" modified="2005-10-11" version="1">MAHKKAGGSSRNGRDSHGKRLGIKAFGGEHVIPGNIIARQRGTTWHPGLNVGMGTDHTLFAKVEGHVAFRVKTGGRTFVSVLPMTEAAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVP8</accession>
    <name>Q3SVP8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Release factor glutamine methyltransferase</fullName>
            <shortName evidence="6">RF MTase</shortName>
            <ecNumber evidence="6">2.1.1.297</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">N5-glutamine methyltransferase PrmC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Protein-(glutamine-N5) MTase PrmC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Protein-glutamine N-methyltransferase PrmC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">prmC</name>
        <name evidence="9" type="ordered locus">Nwi_0376</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 6">Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>L-glutaminyl-[peptide chain release factor] + S-adenosyl-L-methionine = H(+) + N(5)-methyl-L-glutaminyl-[peptide chain release factor] + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42896"/>
            <dbReference type="Rhea" id="RHEA-COMP:10271"/>
            <dbReference type="Rhea" id="RHEA-COMP:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61891"/>
            <dbReference type="EC" id="2.1.1.297"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein N5-glutamine methyltransferase family. PrmC subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.297" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313707.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0376"/>
    <dbReference type="EnsemblBacteria" id="ABA03643">
        <property type="protein sequence ID" value="ABA03643"/>
        <property type="gene ID" value="Nwi_0376"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0376"/>
    <dbReference type="eggNOG" id="COG2890">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018398_3_1_5"/>
    <dbReference type="OMA" id="FDARYWE"/>
    <dbReference type="OrthoDB" id="1816476at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102559">
        <property type="term" value="F:protein-(glutamine-N5) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036009">
        <property type="term" value="F:protein-glutamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018364">
        <property type="term" value="P:peptidyl-glutamine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02126">
        <property type="entry name" value="RF_methyltr_PrmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004556">
        <property type="entry name" value="HemK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040758">
        <property type="entry name" value="PrmC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019874">
        <property type="entry name" value="Release_fac_Glu-N5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17827">
        <property type="entry name" value="PrmC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00536">
        <property type="entry name" value="hemK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03534">
        <property type="entry name" value="RF_mod_PrmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PrmC_N" evidence="8">
        <location>
            <begin position="14"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="MTS" evidence="7">
        <location>
            <begin position="126"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="6">
        <location>
            <begin position="131"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="197"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA03643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="298" mass="30681" checksum="D9F2B81C0A2711C1" modified="2005-10-11" version="1">MADSFAAQTIESIRRALTARFEAAGIESAALDARMLTGAVLDLDLTGLIAQGLRQLDEDDAARLDAFARRRLAGEPIARILGTKEFWGLPLKLSADTLVPRADTETVVEAALGVLRAEGRTQEPLRIADLGTGSGAILLALLTELPKATGVGTDLSAAALDTARANAQGVGLAARSRFLVSDYADGLSGQFDLIVSNPPYIRSADIPTLAPEVRDHDPHLALDGGLDGLDAYRRIAPRAAALLADGGLLVLEIGQGQGGAVTPLVSAAGLTVMGSVRADLAGIGRAVVAGKIALGKPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVX2</accession>
    <name>Q3SVX2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Flp pilus assembly CpaB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0302</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0302"/>
    <dbReference type="EnsemblBacteria" id="ABA03569">
        <property type="protein sequence ID" value="ABA03569"/>
        <property type="gene ID" value="Nwi_0302"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0302"/>
    <dbReference type="eggNOG" id="COG3745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057068_1_1_5"/>
    <dbReference type="OMA" id="FFAKQWM"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017592">
        <property type="entry name" value="Pilus_assmbl_Flp-typ_CpaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031571">
        <property type="entry name" value="RcpC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013974">
        <property type="entry name" value="SAF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16976">
        <property type="entry name" value="RcpC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08666">
        <property type="entry name" value="SAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00858">
        <property type="entry name" value="SAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03177">
        <property type="entry name" value="pilus_cpaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="SAF" evidence="2">
        <location>
            <begin position="56"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="275" mass="29405" checksum="77AD8DB922CADD6B" modified="2005-10-11" version="1">MTATQAPRRNVDNQRMNTARIVVLTIAVGAGGLAAYLASGSDKETQPVQTEQLRTVDVLVAKSDIPLGQAVTANDVAWQTWPADSASHSFIRRDQRPDATTQLAGSIARSPFIAGEPIRELKLVSAKGSGFMAAILPTGMRAISTEISPETGAGGFILPNDRVDVILSKREKNADHASGDSVNSETVLGNVRVLAIDQTIEEKNGQKVVVGRTATLELKPEQAETLARSRQMGTLSLALRSLADANAPEGDINDRRRDSINVVRYGVPTQTSVQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVY1</accession>
    <name>Q3SVY1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cell wall-associated hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0293</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C40 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313626.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0293"/>
    <dbReference type="MEROPS" id="C40.001"/>
    <dbReference type="EnsemblBacteria" id="ABA03560">
        <property type="protein sequence ID" value="ABA03560"/>
        <property type="gene ID" value="Nwi_0293"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0293"/>
    <dbReference type="eggNOG" id="COG0791">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016043_13_3_5"/>
    <dbReference type="OMA" id="NAPYLWG"/>
    <dbReference type="OrthoDB" id="682655at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000064">
        <property type="entry name" value="NLP_P60_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041382">
        <property type="entry name" value="SH3_16"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00877">
        <property type="entry name" value="NLPC_P60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18348">
        <property type="entry name" value="SH3_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SSF50044"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51935">
        <property type="entry name" value="NLPC_P60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NlpC/P60" evidence="2">
        <location>
            <begin position="156"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="281" mass="30355" checksum="CECA173A2FC5D14B" modified="2005-10-11" version="1">MDDPRLTPARPEVAARYLEGRVDAARFTDGEEFEVVEAAAPLQREPAPDAMLQTQALRGERVTVYDHDAEGWAWGQLNGDGYVGWLPDRALARPGPAPTHKVTALRTFAFPGPSIKLPPLETFSLGARLTVQRHDGCFAVTDDRLYIPAPHVAPLDHSAADFVTVAEMFVGTPYLWGGKTSLGIDCSGLVQVALNASGIACPRDSDMQEAAIGRTLSPQEAQQPRRGDLMFWKGHVAIVRNEATLLHANAHHMATAIEGIHDAVTRIKAAGSDVTSVKRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVY4</accession>
    <name>Q3SVY4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Binding-protein-dependent transport systems inner membrane component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0290</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313623.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0290"/>
    <dbReference type="EnsemblBacteria" id="ABA03557">
        <property type="protein sequence ID" value="ABA03557"/>
        <property type="gene ID" value="Nwi_0290"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0290"/>
    <dbReference type="eggNOG" id="COG4174">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036879_1_1_5"/>
    <dbReference type="OMA" id="TFVVIQF"/>
    <dbReference type="OrthoDB" id="1479518at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="227"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="285"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="332"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="136"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="369" mass="41050" checksum="0F4C85F1A8ADFBA1" modified="2005-10-11" version="1">MSAYIVRRILLMLPTLLGILFVSFVVVQFAPGGPVERVIAQLSGSDTGGMSRISGSSGGDFGARGQVGASSDAVNSKYRGAQGLDPAFIKSLEQQFGFDKPAHERFLLMVWNFARFDFGKSYFRDTTVIELIKEKLPVSMSLGIWMTLLTYLISIPLGIRKAVKDGSQFDTWTSAVIIVGFAIPGFLFAILLIVLFAGGSFFSIFPLRGLTSDGWNEFPWYWKILDYFWHLTLPLISMALGAFATMTLLTKNSFLDEIRKQYVMTARAKGCSERQVLYNHVFRNAMLIVIAGFPGAFVHAFFSGSLLIETIFSLDGLGLLGFESVLNRDYPVVFGTLYIFSLVGLVVNLISDLTYMWIDPRIDFEAREV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVZ9</accession>
    <name>Q3SVZ9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phage integrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0275</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313608.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0275"/>
    <dbReference type="EnsemblBacteria" id="ABA03542">
        <property type="protein sequence ID" value="ABA03542"/>
        <property type="gene ID" value="Nwi_0275"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0275"/>
    <dbReference type="eggNOG" id="COG0582">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056713_2_0_5"/>
    <dbReference type="OMA" id="AIMAWGG"/>
    <dbReference type="OrthoDB" id="785253at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyr recombinase" evidence="3">
        <location>
            <begin position="179"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="359" mass="40950" checksum="88A06E081E2F44D6" modified="2005-10-11" version="1">MVAFRMKDGTGKIELRYTYEDTDRHGNVRIYVRRPGMKKVRLRKTPGTPEFLEEYRDALAGRLELPSDKRPVRVPAGKGTLRWLCEQYYRSAEYKRLEPRTQHVRRLILDSICKRDGDKPYALMEPRHLRRRRDEKSDTPEAANGIVKALRQVFTFALENDLAQRNPAKDVPYLKSGSEGFHSWNIEEVRQFEERHSVGSKARLALALLLYTGQRRSDVVALGRQHIRDGWFTFTQVKNRNRKPVTLSIPVRPELKAIIDATPSGNLTYLVTAFGKPFTANGFGNWFRERCDEAGLPHCSAHGLRKAAAARLAELGASESEIMAVTGHTTSKEIVRYTRGARQKVLAANAMARFGENKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW55</accession>
    <name>Q3SW55_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">PfkB</fullName>
            <ecNumber evidence="4">2.7.1.20</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0218</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.1.20" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313553.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0218"/>
    <dbReference type="EnsemblBacteria" id="ABA03486">
        <property type="protein sequence ID" value="ABA03486"/>
        <property type="gene ID" value="Nwi_0218"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0218"/>
    <dbReference type="eggNOG" id="COG0524">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027634_5_1_5"/>
    <dbReference type="OMA" id="APFIAQF"/>
    <dbReference type="OrthoDB" id="1604782at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004001">
        <property type="term" value="F:adenosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PfkB" evidence="3">
        <location>
            <begin position="59"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="333" mass="34845" checksum="9C62B6471232D50B" modified="2005-10-11" version="1">MAAAKYDVLAIGNAIFDVLVRTDEGFLAAHGMTKGGMALIDEARAASIYADMGPATEMSGGSAANTIVGLAGFGARTAYVGKVKDDQIGRLYIHDIRAAKVAFDTPPASDGPATGCSYILVTPDGERTMNTYLGAAQDLSSADIDPDAVAASSILYLEGYLWDPKAAKEAFLKASRIAHDAGRQVALTLSDAFCVDRYRDEFLALMRDGTVDLIFANESELHSLYQTSDFDTALAQLRQDIALGVVTRSEKGCVVATEGGVVAVPACPIDNLVDTTGAGDLFAAGFLFGLVRGASHEDAGRLGALAAAEVIQHIGARPQVSLKELAQANRLPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWA1</accession>
    <name>Q3SWA1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0172</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0172"/>
    <dbReference type="EnsemblBacteria" id="ABA03440">
        <property type="protein sequence ID" value="ABA03440"/>
        <property type="gene ID" value="Nwi_0172"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0172"/>
    <dbReference type="eggNOG" id="COG5375">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093446_1_0_5"/>
    <dbReference type="OMA" id="ADIPHIH"/>
    <dbReference type="OrthoDB" id="1781443at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015221">
        <property type="term" value="F:lipopolysaccharide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010664">
        <property type="entry name" value="LipoPS_assembly_LptC-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026265">
        <property type="entry name" value="LptC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06835">
        <property type="entry name" value="LptC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04409">
        <property type="entry name" value="LptC_YrbK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="230" mass="25039" checksum="5F1E91F67CCC1DF6" modified="2005-10-11" version="1">MYPVQNPTYQAGIQTGLEARFARAARHSRMVRTLRIAVPAAVGLAMAGLIGVAAFNPFRTLANLPLDMDNLVVSGTKITMESPRLAGFTPDKRPYEMQATTATQDVADPDHVELHTLRAKVLMEDRTTVTLNSRTGVFNTKTQVLDLRKDILMLSSTGYEARLTHAIVDMGRGTVTSDQPVDVKLLNGTLTADTMQVTEKGDVVRFENNVVMHLVMDRAPADAGTGGDKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWB2</accession>
    <name>Q3SWB2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glutathione hydrolase proenzyme</fullName>
            <ecNumber evidence="4">2.3.2.2</ecNumber>
            <ecNumber evidence="4">3.4.19.13</ecNumber>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName evidence="4">Glutathione hydrolase large chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="4">Glutathione hydrolase small chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0161</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2 4">
            <text>an S-substituted glutathione + H2O = an S-substituted L-cysteinylglycine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:59468"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="ChEBI" id="CHEBI:143103"/>
            <dbReference type="EC" id="3.4.19.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>an alpha-amino acid + an N-terminal (5-L-glutamyl)-[peptide] = 5-L-glutamyl amino acid + N-terminal L-alpha-aminoacyl-[peptide]</text>
            <dbReference type="Rhea" id="RHEA:23904"/>
            <dbReference type="Rhea" id="RHEA-COMP:9780"/>
            <dbReference type="Rhea" id="RHEA-COMP:9795"/>
            <dbReference type="ChEBI" id="CHEBI:77644"/>
            <dbReference type="ChEBI" id="CHEBI:78597"/>
            <dbReference type="ChEBI" id="CHEBI:78599"/>
            <dbReference type="ChEBI" id="CHEBI:78608"/>
            <dbReference type="EC" id="2.3.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>glutathione + H2O = L-cysteinylglycine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:28807"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:61694"/>
            <dbReference type="EC" id="3.4.19.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Sulfur metabolism; glutathione metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">This enzyme consists of two polypeptide chains, which are synthesized in precursor form from a single polypeptide.</text>
    </comment>
    <comment type="PTM">
        <text evidence="4">Cleaved by autocatalysis into a large and a small subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the gamma-glutamyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.2" evidence="4"/>
    <dbReference type="EC" id="3.4.19.13" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313498.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0161"/>
    <dbReference type="MEROPS" id="T03.001"/>
    <dbReference type="EnsemblBacteria" id="ABA03429">
        <property type="protein sequence ID" value="ABA03429"/>
        <property type="gene ID" value="Nwi_0161"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0161"/>
    <dbReference type="eggNOG" id="COG0405">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014813_0_3_5"/>
    <dbReference type="OMA" id="KATKNMF"/>
    <dbReference type="OrthoDB" id="595845at2"/>
    <dbReference type="UniPathway" id="UPA00204"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036374">
        <property type="term" value="F:glutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102953">
        <property type="term" value="F:hypoglycin A gamma-glutamyl transpeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103068">
        <property type="term" value="F:leukotriene C4 gamma-glutamyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006750">
        <property type="term" value="P:glutathione biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043138">
        <property type="entry name" value="GGT_lsub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000101">
        <property type="entry name" value="GGT_peptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043137">
        <property type="entry name" value="GGT_ssub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00066">
        <property type="entry name" value="g_glut_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00462">
        <property type="entry name" value="G_GLU_TRANSPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229308" description="Glutathione hydrolase proenzyme" evidence="6">
        <location>
            <begin position="32"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="565"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU368036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="584" mass="61102" checksum="53C98A297F0F70AA" modified="2005-10-11" version="1">MIRREIRRYVFFLFAMSVIVAWTPLSSLAFAQDARGAVPAVTSDAIRPVAAKHGMVVAQERLAARIGADVLAQGGNAVDAAVATGFALAVTYPRAGNIGGGGFMLIHLADSSRDIAIDYRETAPGATGRDIFLGADGKPDPAKSRDSALGIGVPGTVAGLALALDRYGSGRFTLARLLQPAITLAREGITVADDSADTLSGSHDRLARWPSSMKIFARPDGAALREGDRLVQTDLAATLTAIAGQGPQGFYEGPVASKLVKGIGAAGGIMTLEDLKSYRPLIREPVRGRYRGYDIISMPLSSSGGTVLLESLNILEGFPMRDLHQGSPDSLHVLVEAMKRAYADRARHLGDPAFVNAPVTTLLAKDYAAKQRASIDMMNATPADAVLASPREGDNTTHFSVADAFGNVVSNTYTLNFSYGVGLVAEGTGVLLNNELDDFTAAPGAANAFGLVGFEANLPGPGKRPLSSMTPTIVLKDGKPVLATGSPGGSRIVSTVLQVIVNVLDYDMNIAAAVQAPRLHHQWLPDEVRAEEGFPDGVIAELRARGHTVVVPMGQTSANSIAITPDGLFGAPDPRTRGSSAAGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWD8</accession>
    <name>Q3SWD8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 2">Protein ApaG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">apaG</name>
        <name evidence="5" type="ordered locus">Nwi_0135</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0135"/>
    <dbReference type="EnsemblBacteria" id="ABA03403">
        <property type="protein sequence ID" value="ABA03403"/>
        <property type="gene ID" value="Nwi_0135"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0135"/>
    <dbReference type="eggNOG" id="COG2967">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1169694_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00791">
        <property type="entry name" value="ApaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007474">
        <property type="entry name" value="ApaG_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036767">
        <property type="entry name" value="ApaG_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023065">
        <property type="entry name" value="Uncharacterised_ApaG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04379">
        <property type="entry name" value="DUF525"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110069">
        <property type="entry name" value="SSF110069"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51087">
        <property type="entry name" value="APAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ApaG" evidence="4">
        <location>
            <begin position="109"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="21"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00017693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="237" mass="26293" checksum="D436E8BED699CDC0" modified="2005-10-11" version="1">MESEARPYNLDLTRFLHANRSPAPRKRYSSDKKAILSPRSSCGTGLAHDHGRSGAGSCASFGYDRPDTIAFSSEVDTGSREENVFNQDLRASLLIPSEAKGSREFAMYRAVTRHIEVTVEPNFMPERSSAERGHFFWSYTVVITNAGPDTVQLRTRHWIITDASGRKQEVRGEGVVGEQPVLAPGERFEYTSGVPLPTATGFMSGRYQMISIRSGEPFEIDVPTFSLDSPDSKRVLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWF4</accession>
    <name>Q3SWF4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_0119</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313456.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0119"/>
    <dbReference type="EnsemblBacteria" id="ABA03387">
        <property type="protein sequence ID" value="ABA03387"/>
        <property type="gene ID" value="Nwi_0119"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0119"/>
    <dbReference type="eggNOG" id="COG0784">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_15_5"/>
    <dbReference type="OMA" id="LADRNWE"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="9">
        <location>
            <begin position="107"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="241"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="8">
        <location>
            <begin position="478"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="8">
        <location>
            <begin position="623"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="588"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="4">
        <location>
            <position position="527"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="4">
        <location>
            <position position="677"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA03387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="754" mass="82511" checksum="65705861F9005977" modified="2005-10-11" version="1">MTARLFWTKARLRARRLIRRHPRLNVAFRTFMAFSIAFGGAYGFIAGSLSADYDPHAFAIGISFLFALACVALAILSMRLRRMRKKMHKIALHNEALVDRNWELKEAEERARHLFESQSDLIVLRDGGGVITSVNEAYCILAQRSRTELLGSRFALPVLEQGESAIESNGTRVYDQRVETARGPRWIAWREALMRIDADHPAELQCVGRDVTDRTDSERALAEARDRANEASQAKSRFLAMTSHEFRTPLNGIIGMSGLLLETLLTPEQTTYVKAVKASGDALLSLIEDLLDYSRIEAGKVELECRPFVLSDLIEDITELLAPRAQARQLEIASYVDETLPAEVIGDAARLRQVLLNLAGNAIKFTSSGGVALIVVPGIRPGDIRFMVRDTGIGIAPDAQARIFREFEQADDQIIRSYGGSGLGLSISDRIVKRMGGRITLQSKPDSGSTFEVLVPLPAQDRAPERKRFTAPDLTGQPVMLVAPQAIETSLLARRLERWGAEICTASDLTVARALLPERAWHAVVVDHAMGGGAIWRFGQAARQHTERRIIMVTPSTRHDLQVAGAAAFTGYLVKPLRTASIAARLSAPADDPTSLRTAGGQPDCGRPETSRTPASTSGPSLSILIAEDNEINALLTRSLLTRLGHAVTVATNGEEALKSWDAALAAGAPYDLVLMDVQMPHLDGIEATRKIRERESVRSGRRTTILALTANALIDDRHACFEAGMDGFLVKPLDRERLADALAGLTAARHVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWI3</accession>
    <name>Q3SWI3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glutathione S-transferase</fullName>
            <ecNumber evidence="4">2.5.1.18</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0090</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313427.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0090"/>
    <dbReference type="EnsemblBacteria" id="ABA03358">
        <property type="protein sequence ID" value="ABA03358"/>
        <property type="gene ID" value="Nwi_0090"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0090"/>
    <dbReference type="eggNOG" id="COG0625">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011226_14_4_5"/>
    <dbReference type="OMA" id="FPITRKW"/>
    <dbReference type="OrthoDB" id="1560019at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="SSF47616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="18"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="108"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="234" mass="26450" checksum="09FB500FEB4A7A6F" modified="2005-10-11" version="1">MADLAAFLITRRWPAAHPERLQLYSVPTPNGVKVSIMLEETGLPYEPHFVDFGRDEQKSREFVSLNPNGRIPAILDPDGPGGKPLGVFESGAILQYLAEKTGQFLPADPARRWQALQWLHFQMGGVGPMFGQVGFFHKFAGRDFQDKRPRDRYVSESKRLLGVMDTHLATSRWFSGDDYTIADIAMLGWVRNLIGFYDARDLVEFDRFRHVGGWLQRGLARPAVQRGLAIPARP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-11-25" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWP3</accession>
    <name>KATG_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Catalase-peroxidase</fullName>
            <shortName evidence="1">CP</shortName>
            <ecNumber evidence="1">1.11.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Peroxidase/catalase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">katG</name>
        <name type="ordered locus">Nwi_0030</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AH2 + H2O2 = A + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:30275"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="EC" id="1.11.1.21"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 H2O2 = 2 H2O + O2</text>
            <dbReference type="Rhea" id="RHEA:20309"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="EC" id="1.11.1.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="1">Binds 1 heme b (iron(II)-protoporphyrin IX) group per dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer or homotetramer.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Formation of the three residue Trp-Tyr-Met cross-link is important for the catalase, but not the peroxidase activity of the enzyme.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peroxidase family. Peroxidase/catalase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.21" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313369.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWP3"/>
    <dbReference type="SMR" id="Q3SWP3"/>
    <dbReference type="STRING" id="323098.Nwi_0030"/>
    <dbReference type="PeroxiBase" id="3657">
        <property type="entry name" value="NwCP01_Nb-255"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03298">
        <property type="protein sequence ID" value="ABA03298"/>
        <property type="gene ID" value="Nwi_0030"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0030"/>
    <dbReference type="eggNOG" id="COG0376">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025424_2_0_5"/>
    <dbReference type="OMA" id="MILAGNC"/>
    <dbReference type="OrthoDB" id="49441at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004096">
        <property type="term" value="F:catalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01961">
        <property type="entry name" value="Catal_peroxid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000763">
        <property type="entry name" value="Catalase_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30555">
        <property type="entry name" value="PTHR30555"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00460">
        <property type="entry name" value="BPEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="SSF48113"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00198">
        <property type="entry name" value="cat_per_HPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0349">Heme</keyword>
    <keyword id="KW-0376">Hydrogen peroxide</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0575">Peroxidase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000354850" description="Catalase-peroxidase">
        <location>
            <begin position="1"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="1">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <feature type="cross-link" description="Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-243)" evidence="1">
        <location>
            <begin position="95"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="cross-link" description="Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-95)" evidence="1">
        <location>
            <begin position="217"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="729" mass="80042" checksum="442BA399F78896AA" modified="2005-10-11" version="1">MDAKTNDGKAGQCPFTSGRGHKNRDWWPEQLDVQVLHQKSNLSNPMGRDFDYASAFASLDLNAVIADLKALMTDSQDWWPADFGHYGGLMVRMAWHSAGTYRITDGRGGAGGGQQRFAPLNSWPDNVLLDRARRLLWPIKQKYGRKISWADLYVLAGNAALESMGFKPLGFAGGRVDEWEPQELFWGPEGAWLGDERYSGERELAEPLAAVQMGLIYVNPEGPNGQPDPLAAAKDIRETFLRMAMNDEETVALIAGGHTFGKTHGAGDASLVGPVPDSAPIEDQGLGWKGRFGTGKGADAIGSGLEVTWTQTPTKWDNNFFDTLFGFEWELTKSPAGAHQWRAKDAPAITPDPFDESKKHVPTMLTTDLSLRFDPIYGEISKRFHENPDQFADAFARAWFKLTHRDMGPRDRYLGPLVPKEVMIWQDPIPAVDHELVDDNDIADLKAKILASGCTVAQLVSTAWASASTFRGSDKRGGANGARIRLAPQKDWEVNQPIQLKAVLAKLEEIQAEFNDAQTGGKKVSLADLIVLGGSAAVEKAARDAGIDLKVPFTPGRMDASLDQTDVESFAPLEPRADGFRNYISDRHQFMAPEEALVDRAQLLNLTGPEMTVLVGGLRVLGANAADSRHGVFTTRPGKLTNDFFVNLLTMDTEWQPVAGQDGVYESRDRKTGTLKWTGTRVDLIFGSHSQLRAFAEVYACADSGEKFANDFAAAWAKVMNADRFDLKA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWQ2</accession>
    <name>RIMP_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosome maturation factor RimP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rimP</name>
        <name type="ordered locus">Nwi_0021</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for maturation of 30S ribosomal subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RimP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313360.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWQ2"/>
    <dbReference type="SMR" id="Q3SWQ2"/>
    <dbReference type="STRING" id="323098.Nwi_0021"/>
    <dbReference type="EnsemblBacteria" id="ABA03289">
        <property type="protein sequence ID" value="ABA03289"/>
        <property type="gene ID" value="Nwi_0021"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0021"/>
    <dbReference type="eggNOG" id="COG0779">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070525_0_0_5"/>
    <dbReference type="OMA" id="PMVRKSD"/>
    <dbReference type="OrthoDB" id="1595498at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01734">
        <property type="entry name" value="YlxS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01077">
        <property type="entry name" value="RimP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003728">
        <property type="entry name" value="Ribosome_maturation_RimP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028998">
        <property type="entry name" value="RimP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036847">
        <property type="entry name" value="RimP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028989">
        <property type="entry name" value="RimP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035956">
        <property type="entry name" value="RimP_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33867">
        <property type="entry name" value="PTHR33867"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02576">
        <property type="entry name" value="DUF150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17384">
        <property type="entry name" value="DUF150_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74942">
        <property type="entry name" value="SSF74942"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75420">
        <property type="entry name" value="SSF75420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="chain" id="PRO_0000229255" description="Ribosome maturation factor RimP">
        <location>
            <begin position="1"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="198"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="214"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="260" mass="28649" checksum="29F3A9A887B2BAD6" modified="2005-10-11" version="1">MTDPSAQNPDHELLDEPRLVVEPGVAARVAAIAAPVLQGMGYRLVRIKVSGEAGCTVQIMAERPDGSIQIEDCEAISKALSPVLDVADPIDKAYRLEISSPGIDRPLVRRSDFERYAGHLAKIEMAVAHQGRKRFRGVLQGVEDNAVRLHRDDIRNNDDESEVMLVMEDIADARLVLTDELIAESMRRGKIAEREMKQSLGILPPPPPHAKTDPAKRGTPKPKLENGKKAPGKIPPKNTKEHRLAAERLRRGDIDPIEGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMV0</accession>
    <name>DNAA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chromosomal replication initiator protein DnaA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dnaA</name>
        <name type="ordered locus">Nwi_0001</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DnaA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313340.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SMV0"/>
    <dbReference type="SMR" id="Q3SMV0"/>
    <dbReference type="STRING" id="323098.Nwi_0001"/>
    <dbReference type="EnsemblBacteria" id="ABA03269">
        <property type="protein sequence ID" value="ABA03269"/>
        <property type="gene ID" value="Nwi_0001"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0001"/>
    <dbReference type="eggNOG" id="COG0593">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026910_3_0_5"/>
    <dbReference type="OMA" id="REFNPLF"/>
    <dbReference type="OrthoDB" id="219876at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06571">
        <property type="entry name" value="Bac_DnaA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1750.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00377">
        <property type="entry name" value="DnaA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001957">
        <property type="entry name" value="Chromosome_initiator_DnaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020591">
        <property type="entry name" value="Chromosome_initiator_DnaA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018312">
        <property type="entry name" value="Chromosome_initiator_DnaA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013317">
        <property type="entry name" value="DnaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013159">
        <property type="entry name" value="DnaA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024633">
        <property type="entry name" value="DnaA_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038454">
        <property type="entry name" value="DnaA_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010921">
        <property type="entry name" value="Trp_repressor/repl_initiator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30050:SF2">
        <property type="entry name" value="PTHR30050:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00308">
        <property type="entry name" value="Bac_DnaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08299">
        <property type="entry name" value="Bac_DnaA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11638">
        <property type="entry name" value="DnaA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00051">
        <property type="entry name" value="DNAA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00760">
        <property type="entry name" value="Bac_DnaA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48295">
        <property type="entry name" value="SSF48295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00362">
        <property type="entry name" value="DnaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01008">
        <property type="entry name" value="DNAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000048682" description="Chromosomal replication initiator protein DnaA">
        <location>
            <begin position="1"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="176"/>
            <end position="183"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00377"/>
        </source>
    </evidence>
    <sequence length="475" mass="52924" checksum="19A8F4F5CACFB646" modified="2005-10-11" version="1">MSDTEQERWSRVKGRLRSTVGEDIYSSWFARMDLESVHGESVRLSVPTRFLKSWIQAHYAERVLSCWQAELPDVHRIDLTVRSAMRCAAPVREAPATDARHPERSEGRNGVELKTVATAPASANHDALGGSPLDPRLTFQSFVTGRSNTLAHAAARQVAEGRRGDSVMFNPLYIHAGVGLGKTHLLQAVTWAGNAGTERRVLYLTAEKFMYGFVAALKTQTALAFKEALRGIDVLVIDDLQFLQGKSTQAEFCHTLNALIDAGRQVVIAADRPPSDLESLDDRVRSRLAGGLVVEMGSLGEELRLGILRSRVEAARVHHASFDVPEPVLDYLAKAITHNGRDLEGAINRLLAHSKLNAQPVTLEMAEREVRDLVRPQEPRRIKIEDIQRVVARQYNVSRSDLLSSRRTANVVRPRQVAMYLAKTLTLRSLPEIGRRFGGRDHTTVLHAVRKIEALVSKDTTLSDEVELLKRQLQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMW8</accession>
    <name>Q3SMW8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Arsenite efflux pump ACR3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_3126</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the arsenical resistance-3 (ACR3) (TC 2.A.59) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316281.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3126"/>
    <dbReference type="EnsemblBacteria" id="ABA06373">
        <property type="protein sequence ID" value="ABA06373"/>
        <property type="gene ID" value="Nwi_3126"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3126"/>
    <dbReference type="eggNOG" id="COG0798">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022869_0_2_5"/>
    <dbReference type="OMA" id="ISAKWYD"/>
    <dbReference type="OrthoDB" id="1493959at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015103">
        <property type="term" value="F:inorganic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004706">
        <property type="entry name" value="Arsenical-R_Acr3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002657">
        <property type="entry name" value="BilAc:Na_symport/Acr3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43057">
        <property type="entry name" value="PTHR43057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01758">
        <property type="entry name" value="SBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005508">
        <property type="entry name" value="Acr3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00832">
        <property type="entry name" value="acr3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="148"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="217"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="247"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="280"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="308"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR005508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA06373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="351" mass="37600" checksum="5F5088AE4C865BDE" modified="2005-10-11" version="1">MSTFERYLTFWVALCIVVGIAFGHVMPGVFQAIGAAEIAKVNLPVAVLIWLMVIPMLLKIDFAALGEVGRHWRGIGVTLFVNWAVKPFSMALLGWLFIGWLFRSLLPADQIDSYIAGLIILAAAPCTAMVFVWSNLTKGEPHFTLSQVALNDAIMVVAFAPIVGLLLGLSAITVPWGTLILSVALYIVIPVIVAQIIRRRLLSSGGQAALDRLLAKLGPVSLVSLLATLVLLFGFQGEQIMAQPMVIALLAVPILIQVYFNSGLAYLLNRISGEQHCVAGPSALIGASNFFELAVAAAISLFGFHSGAALATVVGVLIEVPVMLSVVWIVNRSKGWYERDGKAAAVVEANR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMZ8</accession>
    <name>Q3SMZ8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_3096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06343">
        <property type="protein sequence ID" value="ABA06343"/>
        <property type="gene ID" value="Nwi_3096"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3096"/>
    <dbReference type="HOGENOM" id="CLU_1676039_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="157" mass="16761" checksum="2F9223AD977A8B1E" modified="2005-10-11" version="1">MFPGLGMALPRAGCAKDKRAMTIRRGLRAVPFLAFGAASIWWAARAPHGRKPFAIACDFSLDALLLALTKWPHMASMAFLYLLALIATGLQRSVLAATLVFGVGVGWELAQSTVIGHNARIADLLPNAAALTVCVLVTRIGQMLHRRMAGARALNAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN97</accession>
    <name>Q3SN97_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Thiolase</fullName>
            <ecNumber evidence="6">2.3.1.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_2994</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.16" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316166.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2994"/>
    <dbReference type="EnsemblBacteria" id="ABA06244">
        <property type="protein sequence ID" value="ABA06244"/>
        <property type="gene ID" value="Nwi_2994"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2994"/>
    <dbReference type="eggNOG" id="COG0183">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031026_2_0_5"/>
    <dbReference type="OMA" id="MTAFPEP"/>
    <dbReference type="OrthoDB" id="330994at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003988">
        <property type="term" value="F:acetyl-CoA C-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase_N" evidence="4">
        <location>
            <begin position="5"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase_C" evidence="5">
        <location>
            <begin position="288"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="2">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="383"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA06244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="427" mass="45656" checksum="E9924467CA23F465" modified="2005-10-11" version="1">MVRPVYIIDGSRTPFLKARGGPGPFTPVDLAVQCGRPLLARQPFAPEAFDQVILGCVNVIADEMNPARVAALRLGMGEAMVAFTVQINCGSGMQSMDTGYRYIRDGDANLILTGGAEALSHAPLVWPQQGVRWFAGVAAAKGIVEKVKAIAKARPPYFKPVIGLERGLTDPITELNMGQTAEVVGHLFGITREQSDAYAVESHKRLAKAQAEGWLKGELETAFARDGRFYDHDDGVRPDSSMESLAKLKPVFERPWGQVTAGNSSQITDGACWTILASEDAVIKHGLEPKAMIIDSQWSGLDPSVMGLGPVLSATELLNRNKLSLQDIETWELNEAFATQVLGCLAAWEDEKFCRDVLGLEGAAGAIDRARLNVDGGAVSLGHPVGTSGNRIVLHLVNAMKRLGTKRGIATECIGGGLGGAMLVEMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNC3</accession>
    <name>Q3SNC3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 9">D-3-phosphoglycerate dehydrogenase</fullName>
            <ecNumber evidence="9">1.1.1.95</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_2968</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 9">
            <text>(2R)-3-phosphoglycerate + NAD(+) = 3-phosphooxypyruvate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:12641"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18110"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="EC" id="1.1.1.95"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-2-hydroxyglutarate + NAD(+) = 2-oxoglutarate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:49612"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.399"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 9">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 9">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.95" evidence="9"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316140.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2968"/>
    <dbReference type="EnsemblBacteria" id="ABA06218">
        <property type="protein sequence ID" value="ABA06218"/>
        <property type="gene ID" value="Nwi_2968"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2968"/>
    <dbReference type="eggNOG" id="COG0111">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019796_8_1_5"/>
    <dbReference type="OMA" id="NIAGMQV"/>
    <dbReference type="OrthoDB" id="1638924at2"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00196"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004617">
        <property type="term" value="F:phosphoglycerate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029009">
        <property type="entry name" value="ASB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006236">
        <property type="entry name" value="PGDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045626">
        <property type="entry name" value="PGDH_ASB_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19304">
        <property type="entry name" value="PGDH_inter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143548">
        <property type="entry name" value="SSF143548"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01327">
        <property type="entry name" value="PGDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00670">
        <property type="entry name" value="D_2_HYDROXYACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="7 9 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0718">Serine biosynthesis</keyword>
    <feature type="domain" description="ACT" evidence="10">
        <location>
            <begin position="457"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA06218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="529" mass="56087" checksum="F135F885E2186FBB" modified="2005-10-11" version="1">MTKPKVLISDALSPAAVQIFKDRGIEVDFQPALGKDKDKLAEIIGDYDGLAIRSATKVTAKILANARKLKVVGRAGIGVDNVEIPAATAKGVIVMNTPFGNSITTAEHAITLMLALAREIPAADASTQAGKWEKNRFMGVEITGKTLGVIGCGNIGSIVADRALGLKMKVVAFDPFLSPERARDIGVEKVELDDLFKRADFITLHTPLTDKTRNIIDADAIAGMKKGVRIINCARGGLVDEQALADALNTKQVAGAAFDVFVEEPATGNVLFGHPNVICTPHLGASTTEAQENVALQVAEQMSDYLLTGAITNALNFPSITAEEAPKLKPFVELAEKLGSFAGQLTETGISKVTITYEGQVAEMKIKALTSAALSGLLRPMLGDVNVVSAPVVAKERGMVVDEVVRAAQSDYESLITVTVFTERQERSVSGTVYADGHPRLVDIKGIRVDAEFGKSMIYVTNEDRPGFIGKFASLLGDAGVNIATFNLGRHSQGGDAIALVEVDGSVPENVLAKVQALPQVRQAKALTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SND0</accession>
    <name>Q3SND0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2961</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316133.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06211">
        <property type="protein sequence ID" value="ABA06211"/>
        <property type="gene ID" value="Nwi_2961"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2961"/>
    <dbReference type="eggNOG" id="ENOG503392M">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_180799_0_0_5"/>
    <dbReference type="OMA" id="YRRFDNA"/>
    <dbReference type="OrthoDB" id="1892056at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="95" mass="10791" checksum="D40F72943917D641" modified="2005-10-11" version="1">MTFSFQKSAELFPAAVRRKKRAGFSYRRFDTAADAVRFAIEELPADSLNGAYLQVDEARFDQNGIRSLYESRDFPLPRRKLDTASAETRVPKDAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SND4</accession>
    <name>Q3SND4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Lytic murein transglycosylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2957</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2957"/>
    <dbReference type="CAZy" id="GH103">
        <property type="family name" value="Glycoside Hydrolase Family 103"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06207">
        <property type="protein sequence ID" value="ABA06207"/>
        <property type="gene ID" value="Nwi_2957"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2957"/>
    <dbReference type="eggNOG" id="COG2951">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035402_0_2_5"/>
    <dbReference type="OMA" id="WPADGYP"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.101.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043426">
        <property type="entry name" value="MltB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011970">
        <property type="entry name" value="MltB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036366">
        <property type="entry name" value="PGBDSf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031304">
        <property type="entry name" value="SLT_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30163">
        <property type="entry name" value="PTHR30163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13406">
        <property type="entry name" value="SLT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="SSF47090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02283">
        <property type="entry name" value="MltB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229105" evidence="2">
        <location>
            <begin position="24"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="SLT_2" evidence="4">
        <location>
            <begin position="100"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="PG_binding_1" evidence="3">
        <location>
            <begin position="412"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA06207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="470" mass="51370" checksum="E8EC1F1FC9FFA247" modified="2005-10-11" version="1">MRGTGMAAVVALAVLAPPGEARAQASNGNGIQGFLDNIFTGSIANDGQSASLAPRGGSEQTPSPPKRPASSAATAWSGEDGASGHPLMTAAAIRQAAANFSNCVAAMWPDAARRHITRENFERFTAGLSPDLRIMDLMDSQPEFTKAIWDYLDILVKDARLARGREILATYKPQFDAVEKAYGVDRYAIAAIWGIESNYSTQMGDRSVLQSTATLACVGRRQKYFRDEFLAALEILNRGDLRPEQLRGSWAGAFGPTQFMPTAFKRYAVDGDGDGRRDVVDNPADLIASTANNLKKDGWQTGRTWGYEVVVPNDFNYMLADRAKAMTLAQWEQLGIRRAGNKAFPYSADRAYLLAPAGAGGPGFLMLQNFRVFMKYNPAEAYALAIGHFADRLRGGSPFVQPWPRQERVLSRAERLELQQLLARRGFYRGTPDGQIGSRTREALRGFQASVGAPADGFASSDVLERLRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SND7</accession>
    <name>Q3SND7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glutamate synthase (NADH) small subunit</fullName>
            <ecNumber evidence="7">1.4.1.14</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2954</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.14" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316126.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2954"/>
    <dbReference type="EnsemblBacteria" id="ABA06204">
        <property type="protein sequence ID" value="ABA06204"/>
        <property type="gene ID" value="Nwi_2954"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2954"/>
    <dbReference type="eggNOG" id="COG0493">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_3_1_5"/>
    <dbReference type="OMA" id="DCVGTAH"/>
    <dbReference type="OrthoDB" id="671513at2"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00109"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016040">
        <property type="term" value="F:glutamate synthase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028261">
        <property type="entry name" value="DPD_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006005">
        <property type="entry name" value="Glut_synth_ssu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14691">
        <property type="entry name" value="Fer4_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01317">
        <property type="entry name" value="GOGAT_sm_gam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0314">Glutamate biosynthesis</keyword>
    <keyword evidence="2 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fer4_20" evidence="6">
        <location>
            <begin position="23"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Pyr_redox_2" evidence="5">
        <location>
            <begin position="154"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA06204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="484" mass="52994" checksum="EA5B129F073CE917" modified="2005-10-11" version="1">MGKITGFLEIERHDRKYAPVEERVKNFKEFVIPLSEKETRDQAARCMNCGIPYCHGTGSVQPGTPGCPVNNQIPDFNDLVYQGNWEEASRTLHSTNNFPEFTGRICPAPCEASCTLNIDDNPVTIKTIECAIVDRAWDNGWLKPVVAAQKTGRKIAVVGSGPAGLACAQQLARVGHEVHVYEKNAKAGGLLRYGIPDFKMEKHIIDRRLEQMQAEGVIFHYGKPVGGDATGAIDPVQLVRDHDAVALTGGAEAPRDLPIPGRDLDGIHFAMDFLPQQNRRNGNETFDTPDILATGKNVVVIGGGDTGSDCIGTSFRQGARSVTQLEIMPAPPEHENKALTWPNWPLKMRTSSSQAEGARRDYAVLTQKFSGIDGKVTRLHCVRVDGRFQPIAGTEFALEADLVLLAMGFVHPVYEGLLKTLGLDLDQRGNVRANTQDFQTSLPKVFSAGDMRRGQSLVVWAIREGRLCARAIDQMLMGSTTLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNE1</accession>
    <name>Q3SNE1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Histidinol-phosphate phosphatase, putative, inositol monophosphatase</fullName>
            <ecNumber evidence="3">3.1.3.25</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2950</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.25" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316122.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2950"/>
    <dbReference type="EnsemblBacteria" id="ABA06200">
        <property type="protein sequence ID" value="ABA06200"/>
        <property type="gene ID" value="Nwi_2950"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2950"/>
    <dbReference type="eggNOG" id="COG0483">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044118_4_1_5"/>
    <dbReference type="OMA" id="WRTRAYG"/>
    <dbReference type="OrthoDB" id="1741160at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004401">
        <property type="term" value="F:histidinol-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008934">
        <property type="term" value="F:inositol monophosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052832">
        <property type="term" value="F:inositol monophosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052833">
        <property type="term" value="F:inositol monophosphate 4-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046855">
        <property type="term" value="P:inositol phosphate dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011809">
        <property type="entry name" value="His_9_proposed"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02067">
        <property type="entry name" value="his_9_HisN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="261" mass="28595" checksum="609E6FDAC545004B" modified="2005-10-11" version="1">MTVIDFTAFIDRLATASGETILPFFRTSLLDVENKSASRDFDPVTEADRAAEAVMRRMIQASFPEHGIVGEEFGNQNEDAEYVWMLDPIDGTKSFIAGFPIWGTLIALLHRGTPVFGMMHQPYIGERFSGDNRSSRYRGPSQGRKLAVRRCPSLQEATSFTTSPLLMNPDDRAAFGKVEAEVRLTRYGGDCYSYCMLAAGHLDLVIETELKSYDIAALIPIITGAGGVITTWEGEPAQGGGRVVAAGDRRVHEAALKRLNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNF8</accession>
    <name>Q3SNF8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ParB-like nuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2933</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2933"/>
    <dbReference type="REBASE" id="13849">
        <property type="entry name" value="M.NwiORF2933P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06183">
        <property type="protein sequence ID" value="ABA06183"/>
        <property type="gene ID" value="Nwi_2933"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2933"/>
    <dbReference type="eggNOG" id="COG1475">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1516368_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB/Sulfiredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02195">
        <property type="entry name" value="ParBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00470">
        <property type="entry name" value="ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="SSF110849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ParB" evidence="1">
        <location>
            <begin position="16"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="177" mass="19023" checksum="94FA6229AE7308E5" modified="2005-10-11" version="1">MSDPVSVASSSRLAVVYRPVNDLTPDPRNARTHPKRQIDQLRASIKEFGFTNPILIDPEGHIIAGHGRLQAARAIGIAEVPTIALAGLSEPQKRALRIADNKIALNAGWDLEILQCPPSGLLRQFRSFREGRISGSSQPIWSADETEIRAGESTGRAGREGHPPGNAPVVMISVAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNN7</accession>
    <name>Q3SNN7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2854</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2854"/>
    <dbReference type="EnsemblBacteria" id="ABA06104">
        <property type="protein sequence ID" value="ABA06104"/>
        <property type="gene ID" value="Nwi_2854"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2854"/>
    <dbReference type="eggNOG" id="COG5340">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089333_0_1_5"/>
    <dbReference type="OMA" id="CFKYRNK"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025159">
        <property type="entry name" value="AbiEi_4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13338">
        <property type="entry name" value="AbiEi_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="232" mass="26336" checksum="FA99E56DB1B94379" modified="2005-10-11" version="1">MPWRPLLKKQIDQSFAGIARITTYLQVETICLMLKTARSEDIFRAHGGQLRMSEAIRHGLSRYTLYALKEHGAIEQISRGLYRLADLPPIGNPDLVTVSLRCPHAVICLVSALSWHGITTEIPHAVPVAVPRDARAFPRSTIPRSSRIGFRMRRSKPASRAMTSMACQSVSTMPRSRSPIASSSATRSEWTWFWEALQLYRQRKRFDPGKLVKYAKVCRVENVMRPYLEAIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNW8</accession>
    <name>Q3SNW8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2770</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2770"/>
    <dbReference type="EnsemblBacteria" id="ABA06023">
        <property type="protein sequence ID" value="ABA06023"/>
        <property type="gene ID" value="Nwi_2770"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2770"/>
    <dbReference type="HOGENOM" id="CLU_2570363_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="81" mass="8596" checksum="376FDCB3344E9EE5" modified="2005-10-11" version="1">MSLALRRRDSLREKDPRDPGFISKILAAGRSGVFGPRRGAPADKRSVHAFSGIAVNDIRRTALMQGGSASATGGCRNRCGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNX8</accession>
    <name>Q3SNX8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Thiosulphate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2760</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the prokaryotic sulfate-binding protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315958.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2760"/>
    <dbReference type="EnsemblBacteria" id="ABA06013">
        <property type="protein sequence ID" value="ABA06013"/>
        <property type="gene ID" value="Nwi_2760"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2760"/>
    <dbReference type="eggNOG" id="COG1613">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055615_0_1_5"/>
    <dbReference type="OMA" id="KDFGGWK"/>
    <dbReference type="OrthoDB" id="1111285at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901681">
        <property type="term" value="F:sulfur compound binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902358">
        <property type="term" value="P:sulfate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01005">
        <property type="entry name" value="PBP2_CysP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034408">
        <property type="entry name" value="Sulphate/thiosulphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005669">
        <property type="entry name" value="Thiosulph/SO4-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30368">
        <property type="entry name" value="PTHR30368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00971">
        <property type="entry name" value="3a0106s03"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00757">
        <property type="entry name" value="PROK_SULFATE_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0574">Periplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229068" evidence="6">
        <location>
            <begin position="21"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA06013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="329" mass="36412" checksum="7E72994561F27D97" modified="2005-10-11" version="1">MLRRILTVLAGLIWAGSAYAADVSLLNVSYDPTRELYVDFNRAFAAHYQKETGKSVEIRQSHGGSGKQARSVIDGLQADVVTLALAYDIDAVADHGLLAKDWQARLPENSSPYTSTIVFLVRTGNPKGIKDWDDLVRPGVNVITPNPKTSGGARWNYLAAWGYALKKWGGEDKARQFVADIYSHVPVLDTGARGSTVTFVERGVGDVLLAWENEAFLALKEFGKDKFEIVAPSISILAEPPVAVVDKVVDKKGTRAAAEAYLKYWYTEEAQDIAARNYYRPTNPGLIKKYADAFPKVELFRIDEVFGGWTKAQKVHFSEGGIFDQIYKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNZ5</accession>
    <name>Q3SNZ5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2743</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315941.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2743"/>
    <dbReference type="EnsemblBacteria" id="ABA05996">
        <property type="protein sequence ID" value="ABA05996"/>
        <property type="gene ID" value="Nwi_2743"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2743"/>
    <dbReference type="eggNOG" id="COG1511">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034730_0_0_5"/>
    <dbReference type="OMA" id="GHGQNRF"/>
    <dbReference type="OrthoDB" id="1344010at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="55"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="194"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="329"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="358" mass="38736" checksum="C8F2FEA49D744AE7" modified="2005-10-11" version="1">MAQGTAPRSAPEIELTKLAPPRIFLVRMVVFIVLCALVLVVLYKQIVAAFFANPSLNALIIGVLAIGIVFAFRQVIRLYPEIAWVNNFRIANAGRVPDRRPRLLAPMAAILGGERTGRVSISQQTMRHLLDSIATRLDEARDISRYMTGLLVFLGLLGTFWGLIETVGSVGKVIDGLKVGGDTGSLFETLKEGLAAPLGGMGISFSSSLFGLAGSLILGFLDLQSSQAQNRFYTDLEDWLATTVREYSGNKTRPDPAAEIKAAIERLSTTVEKTGSGHETTSAMASLAEAIQGLVSHMRTEQQLIREWADEQGEQNREIKKLLQHLAQQPDEDPLTVPLAATTASRPVAPSAEGEAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP02</accession>
    <name>Q3SP02_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7 10">Phosphoglycerate kinase</fullName>
            <ecNumber evidence="2 7 10">2.7.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pgk</name>
        <name evidence="11" type="ordered locus">Nwi_2736</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7 10">
            <text>(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 10">Belongs to the phosphoglycerate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.3" evidence="2 7 10"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315934.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2736"/>
    <dbReference type="EnsemblBacteria" id="ABA05989">
        <property type="protein sequence ID" value="ABA05989"/>
        <property type="gene ID" value="Nwi_2736"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2736"/>
    <dbReference type="eggNOG" id="COG0126">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025427_0_2_5"/>
    <dbReference type="OMA" id="DMIFDIG"/>
    <dbReference type="OrthoDB" id="462069at2"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00185"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004618">
        <property type="term" value="F:phosphoglycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1260">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00145">
        <property type="entry name" value="Phosphoglyc_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001576">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015911">
        <property type="entry name" value="Phosphoglycerate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015824">
        <property type="entry name" value="Phosphoglycerate_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036043">
        <property type="entry name" value="Phosphoglycerate_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406">
        <property type="entry name" value="PTHR11406"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00162">
        <property type="entry name" value="PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000724">
        <property type="entry name" value="Pgk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00477">
        <property type="entry name" value="PHGLYCKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53748">
        <property type="entry name" value="SSF53748"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00111">
        <property type="entry name" value="PGLYCERATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5 7 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 10" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="7 8">
        <location>
            <begin position="23"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7 8">
        <location>
            <begin position="61"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 8">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 8">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 8">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <begin position="354"/>
            <end position="357"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA05989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="398" mass="41757" checksum="111EC4FBC5939DCA" modified="2005-10-11" version="1">MTNSFRTLDDVDVRGKRVLLRVDLNVPMEQGRVTDATRLERVAPTITEIADKGGKAILLAHFGRPKGADPKNSLRPVAEELAKVLGKPVAFADDCVGEPAQQAVAAMKDGDILCLENTRFHPEEEKNDPAFVAELARLGDIWVNDAFSAAHRAHASTEGLGHKLPGYAGRTMQAELEALNKALEAPTKPVIAVIGGAKVSTKIDLLENLVSKVDALVIGGGMANTFLHAQGVSIGKSLAEKDLAATALRIMEKAEAANCAIILPVDATVAFHFAANTPSHAYGLDAIPADGMILDVGPQSVERVRSAIDDAATLVWNGPLGAFELTPFDRGTVAAAKHAAERTKAGKLVSVAGGGDTVAALNQADVAGDFTYVSTAGGAFLEWMEGKPLPGVEVLRAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP41</accession>
    <name>Q3SP41_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">4-phosphoerythronate dehydrogenase (FAD-dependent)</fullName>
            <ecNumber evidence="3">1.1.2.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2697</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.2.4" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2697"/>
    <dbReference type="EnsemblBacteria" id="ABA05950">
        <property type="protein sequence ID" value="ABA05950"/>
        <property type="gene ID" value="Nwi_2697"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2697"/>
    <dbReference type="eggNOG" id="COG0277">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017779_4_1_5"/>
    <dbReference type="OMA" id="CNDNMLA"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004458">
        <property type="term" value="F:D-lactate dehydrogenase (cytochrome) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-linked_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="SSF55103"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="2">
        <location>
            <begin position="50"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="480" mass="50967" checksum="46C4226CE6983F65" modified="2005-10-11" version="1">MDLPIAISKSAQPPLSPDLIARLAAIVGDKYAVTDADELAPYLTEARNLFHGRSPLVLRPASTAEVSAICRLASEHRIALVPQGGNTGLVGGQTPHNGEVVVSMRRMDKIREVDTASNTMTVEAGAILQAIQRRADEAGRFFPLSLGAEGSCTIGGNLSTNAGGTGALAYGVARDMALGLEVVLPDGRTLNGLSKLKKDNTGYDLRNLFIGAEGTLGIITAAVVKLFPKPRSVQTAFVGLRTPADALRLLAISQAEAAGSLTSFELLAEICVDICVRHGSGIRDPLTRRYPWYVLMEVSSSRDDATATVETILERGIKSGCIEDAAIAASLQQRAAFWKLRETIPPAQKREGGSIKHDISVPVAAIPAFIAEANAAVVKLIPDARPVPFGHLGDGNIHYNVSQPVSGMTTHEFLARWQEVNAVVFAIALRMGGSISAEHGIGVLKRDDLPRVKDKTAIETMRGIKALFDPLGIMNPGKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPC4</accession>
    <name>Q3SPC4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Anthranilate synthase</fullName>
            <ecNumber evidence="1">4.1.3.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2614</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>chorismate + L-glutamine = anthranilate + H(+) + L-glutamate + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:21732"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="4.1.3.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.27" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315817.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2614"/>
    <dbReference type="MEROPS" id="C26.A09"/>
    <dbReference type="EnsemblBacteria" id="ABA05867">
        <property type="protein sequence ID" value="ABA05867"/>
        <property type="gene ID" value="Nwi_2614"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2614"/>
    <dbReference type="eggNOG" id="COG0147">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0512">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006493_3_0_5"/>
    <dbReference type="OMA" id="AYRSFMN"/>
    <dbReference type="OrthoDB" id="1786019at2"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00040"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01743">
        <property type="entry name" value="GATase1_Anthranilate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005801">
        <property type="entry name" value="ADC_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019999">
        <property type="entry name" value="Anth_synth_I-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006805">
        <property type="entry name" value="Anth_synth_I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015890">
        <property type="entry name" value="Chorismate_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010112">
        <property type="entry name" value="TrpE-G_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006221">
        <property type="entry name" value="TrpG/PapA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236">
        <property type="entry name" value="PTHR11236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04715">
        <property type="entry name" value="Anth_synt_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00425">
        <property type="entry name" value="Chorismate_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036934">
        <property type="entry name" value="TrpE-G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56322">
        <property type="entry name" value="SSF56322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01815">
        <property type="entry name" value="TrpE-clade3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00566">
        <property type="entry name" value="trpG_papA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="1 5" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Anth_synt_I_N" evidence="4">
        <location>
            <begin position="118"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Chorismate_bind" evidence="3">
        <location>
            <begin position="246"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="GATase" evidence="2">
        <location>
            <begin position="529"/>
            <end position="702"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR036934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="727" mass="79687" checksum="DAAF00B0CEFC2378" modified="2005-10-11" version="1">MNRTAFSLPAQSEYLTAGGLSISRSIEHFTGGSRLDDLIGLLDSRRGVVLSSGTTVPGRYESFDLGFADPPLKIETTGFNFTIEAANARGEVLIAFLGETLSEPCAVVTEKTARRIAGHIVRGDAPVEEDQRTRRASVMSLVRALVAAFASTADPMLGLYGAFAYDLVFQIEDLVQKRAREPDQRDIVLFIPDRLLAYDRAAGHGVVLSYDFAWKGRSTEGQPRDTPDSVYAKTSRQGFTDHAPGEYQATVETARAAFARGDLFEAVPGQLFAEPCERSPAEVFQRLCRINPSPYGGLVNLGDGEFLVSASPEMFVRSDGRRIETCPISGTIARGADAIGDAEQIRELLNSEKDEFELNMCTDVDRNDKARICMPGTIKVLARRQIETYSKLFHTVDHVEGILRSGFDALDGFLTHAWAVTVTGAPKKWAIQFVEDNERSTRRWYAGAFGVVGFDGSINTGLTIRTIRMKDGLAEVRVGATCLFDSKPELEDRECQTKAAALFQALRGDAPKPLSAVAPDATGSGKRVLLIDHDDSFVHMLADYFRQVGARVSVVRYVHARDMLDRQGYDLLVLSPGPGRPEDFGMARTIGLALEKKMPVFGVCLGLQGIGEYFGGRLGQLAQPAHGRPSRVQNRGGRLMRNLPNEIVIGRYHSLYVERDRLPEVLEITATTDDGVPMAIEHKSLPVGGVQFHPESLMSLGGEVGLRIVENAFRLGRERRENSNDAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPF6</accession>
    <name>Q3SPF6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cytochrome c, class I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_2582</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315786.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2582"/>
    <dbReference type="EnsemblBacteria" id="ABA05835">
        <property type="protein sequence ID" value="ABA05835"/>
        <property type="gene ID" value="Nwi_2582"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2582"/>
    <dbReference type="eggNOG" id="COG3474">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060944_2_1_5"/>
    <dbReference type="OMA" id="YSDAMKN"/>
    <dbReference type="OrthoDB" id="1853490at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002327">
        <property type="entry name" value="Cyt_c_1A/1B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11961">
        <property type="entry name" value="PTHR11961"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00034">
        <property type="entry name" value="Cytochrom_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00604">
        <property type="entry name" value="CYTCHRMECIAB"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229090" evidence="6">
        <location>
            <begin position="25"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="7">
        <location>
            <begin position="25"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA05835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="133" mass="14032" checksum="C0C653A472F6528B" modified="2005-10-11" version="1">MKTFAISALTVLGVTLASVGTSSAGDVEAGKSAFNKCKACHEIGEGAKNKVGPELNGLDGRHSGAVEGYAYSPANKASGITWTEAEFKEYIKDPKAKVPGTKMVFAGIKKDSELDNLWAYVSQFDKDGKVKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPJ4</accession>
    <name>Q3SPJ4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2544</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2544"/>
    <dbReference type="EnsemblBacteria" id="ABA05797">
        <property type="protein sequence ID" value="ABA05797"/>
        <property type="gene ID" value="Nwi_2544"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2544"/>
    <dbReference type="eggNOG" id="ENOG5031PVX">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1140694_0_0_5"/>
    <dbReference type="OMA" id="NWPKDPD"/>
    <dbReference type="OrthoDB" id="1376590at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229093" evidence="2">
        <location>
            <begin position="43"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="239" mass="25533" checksum="3D96B5E81183D21F" modified="2005-10-11" version="1">MRNTEQNGWDVLGAAATLLRGARLATVAAGLAVVLSAGMAHAQDDDNTFEEGIIKKVMRGLGATSMENSDIEYRERSPLVIPPSTQLPPPGSGLAATEANDPNWPKDPDVQRRREAAAARKDRTLLLPWENGQSILPSKLAKGSAASKRAETNQGSEDSSINNPSLSPSQLGFVGKLSNLFGGKKTETAPFKGEPEREKLTQPPVGYQTPSPNYAYGTGPKEALGNTYIDVNSGKERTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPK2</accession>
    <name>Q3SPK2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TadE-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2536</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315740.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2536"/>
    <dbReference type="EnsemblBacteria" id="ABA05789">
        <property type="protein sequence ID" value="ABA05789"/>
        <property type="gene ID" value="Nwi_2536"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2536"/>
    <dbReference type="eggNOG" id="COG4961">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128173_2_1_5"/>
    <dbReference type="OMA" id="PIWNLFP"/>
    <dbReference type="OrthoDB" id="1888885at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012495">
        <property type="entry name" value="TadE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07811">
        <property type="entry name" value="TadE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="146" mass="15081" checksum="A66E9706854CDFD4" modified="2005-10-11" version="1">MTGLSICHASSLPRFARCARAASAVEFAMLLPLFLVLVAGIVVFGAYLTMVHGVQQLAAEAARSSVAGLSETERTSLAENYVTTNAGSYPLLQPGHLTMSAATSGGGVFVVTVNYDASDSIIFTLPFVPAPPSTIVRSAAIPFGGF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-05-25" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPL2</accession>
    <name>UVRC_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UvrABC system protein C</fullName>
            <shortName evidence="1">Protein UvrC</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Excinuclease ABC subunit C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">uvrC</name>
        <name type="ordered locus">Nwi_2526</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with UvrB in an incision complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UvrC family.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="ABA05779" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05779.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SPL2"/>
    <dbReference type="SMR" id="Q3SPL2"/>
    <dbReference type="STRING" id="323098.Nwi_2526"/>
    <dbReference type="EnsemblBacteria" id="ABA05779">
        <property type="protein sequence ID" value="ABA05779"/>
        <property type="gene ID" value="Nwi_2526"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2526"/>
    <dbReference type="eggNOG" id="COG0322">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014841_3_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009380">
        <property type="term" value="C:excinuclease repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009381">
        <property type="term" value="F:excinuclease ABC activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00203">
        <property type="entry name" value="UvrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000305">
        <property type="entry name" value="GIY-YIG_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035901">
        <property type="entry name" value="GIY-YIG_endonuc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001943">
        <property type="entry name" value="UVR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036876">
        <property type="entry name" value="UVR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004791">
        <property type="entry name" value="UvrC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001162">
        <property type="entry name" value="UvrC_RNase_H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038476">
        <property type="entry name" value="UvrC_RNase_H_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01541">
        <property type="entry name" value="GIY-YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08459">
        <property type="entry name" value="UvrC_HhH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00465">
        <property type="entry name" value="GIYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46600">
        <property type="entry name" value="SSF46600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82771">
        <property type="entry name" value="SSF82771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00194">
        <property type="entry name" value="uvrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50164">
        <property type="entry name" value="GIY_YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50165">
        <property type="entry name" value="UVRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0228">DNA excision</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0267">Excision nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0742">SOS response</keyword>
    <feature type="chain" id="PRO_0000264918" description="UvrABC system protein C">
        <location>
            <begin position="1"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="domain" description="GIY-YIG" evidence="1">
        <location>
            <begin position="83"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="UVR" evidence="1">
        <location>
            <begin position="271"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="38"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="689" mass="75922" checksum="C816CC93ECFA3A72" modified="2006-12-12" version="2">MTSDSSDTAKQIGSGQPSGSPADMRRRDGVAPEQEVDPASLETDEDDEARLPDLPDEPVDAVAEAPLAIGRAAIEHAVRHAPTSPGVYRMMNAARDVLYVGKAKNVRKRLSSYARPTGQVMRIARMIAATAAVEIVSTGTETEALLLEANLIKQLRPRFNVQLRDDKSFPYILITGDHWAPQLLKHRGAQSRPGRYFGPFASAGAVGRTITALQRAFLVRSCTDSFFESRTRPCLLYQIKRCAGPCTGEIDFPGYTALVREATDFLSGRSRAVKEDLARAMEQAAADLAFERAALYRDRLAALSAIQSQQGINPRTVEEADVFAIHQEGGYFCVEVFFFRTGQNWGNRAYFPRAEKSFTPEEVLGSFLAQFYDDKPPPRLILLSHRIEESELLAHALSIKAGCKVVVSTPQRGEKKELVAHALTNAREALGRKLADTATQSRLLQGLAALLGLPQPPQRVEVYDNSHIQGANAVGAMIVAGPEGFLKNQYRKFNIRSESLTPGDDYAMMREVLERRFKRLLTQKAADSRAAAEQDSAPQWPDLVIIDGGLGQLNAARGVVDALGLSRVSLMAVAKGPDRDAGRETLFLPDRPAIKLEPRDPVLYFIQRLRDEAHRFVIGSHRKLRRKDIREAGLQEVPGIGPARKRALLHHFGTLKEIERASLADLGKVPGISAESARRIFDFFHARPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPL3</accession>
    <name>Q3SPL3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="14">CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase</fullName>
            <ecNumber evidence="14">2.7.8.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Nwi_2525</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="11">Phospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.5" evidence="14"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315729.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2525"/>
    <dbReference type="EnsemblBacteria" id="ABA05778">
        <property type="protein sequence ID" value="ABA05778"/>
        <property type="gene ID" value="Nwi_2525"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2525"/>
    <dbReference type="eggNOG" id="COG0558">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051314_2_1_5"/>
    <dbReference type="OMA" id="WSMVYYL"/>
    <dbReference type="OrthoDB" id="1702107at2"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008444">
        <property type="term" value="F:CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004570">
        <property type="entry name" value="Phosphatidylglycerol_P_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000847">
        <property type="entry name" value="Phos_ph_gly_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00560">
        <property type="entry name" value="pgsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="19"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="89"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="175"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABA05778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="205" mass="22489" checksum="22A2963B1E554BAA" modified="2005-10-11" version="1">MNTATSQATAKHMLSLPNILTYARIAAIPVVVGCVYAQSIMDGPLQLRWVALGVFVAAAVTDFFDGYYARIWDQQSALGKMLDPIADKLLVASCLLMLAADGIIHGWTLWAAIVILCREILVSGLREYLAALRVSVPVTKLAKWKTTVQLVAIGFLLAGEAGDLIFPFVTQIGLLLLWISAILTIYTGWDYFHAGIHHLMEEDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPP1</accession>
    <name>Q3SPP1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2497</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05750">
        <property type="protein sequence ID" value="ABA05750"/>
        <property type="gene ID" value="Nwi_2497"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2497"/>
    <dbReference type="eggNOG" id="ENOG5033KXI">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060262_0_0_5"/>
    <dbReference type="OMA" id="GSAWARM"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229170" evidence="2">
        <location>
            <begin position="28"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="296" mass="31395" checksum="98F605FECA84B97C" modified="2005-10-11" version="1">MASLMRRAAGLLLLVAAAGWTTGTLPARSEAPDNRDNRPEQAAFPGEQPVDSNIDWSQLDVDGFTLTEDMPPQVLRSQRSAPAASGEGLAWTSHDKADGSAALTAKRSVSSLWNAQVGTDLTVIRQPSPLTASEQAAGRLAANAPPSRSSGQAWAAVTAPGLGRIWDKTALEARIDPARDQTMLDTSLSKSLPLSDGRYALTLRNGYNIIQQDALSVPGIKGTRHYNTDHSARLSVTDTGTSLIAGQSLSSTDSRWLRRIGAEQKLSDGLSITGAVSETPQGPLNASLTAAFRRKW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPS2</accession>
    <name>THIG_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thiazole synthase</fullName>
            <ecNumber evidence="1">2.8.1.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thiG</name>
        <name type="ordered locus">Nwi_2466</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + [sulfur-carrier protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH = 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS]-C-terminal Gly-Gly + 2 H(+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:26297"/>
            <dbReference type="Rhea" id="RHEA-COMP:12908"/>
            <dbReference type="Rhea" id="RHEA-COMP:12909"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
            <dbReference type="ChEBI" id="CHEBI:62899"/>
            <dbReference type="ChEBI" id="CHEBI:77846"/>
            <dbReference type="ChEBI" id="CHEBI:90619"/>
            <dbReference type="ChEBI" id="CHEBI:90778"/>
            <dbReference type="EC" id="2.8.1.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer. Forms heterodimers with either ThiH or ThiS.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ThiG family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.10" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315674.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SPS2"/>
    <dbReference type="SMR" id="Q3SPS2"/>
    <dbReference type="STRING" id="323098.Nwi_2466"/>
    <dbReference type="EnsemblBacteria" id="ABA05719">
        <property type="protein sequence ID" value="ABA05719"/>
        <property type="gene ID" value="Nwi_2466"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2466"/>
    <dbReference type="eggNOG" id="COG2022">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062233_1_0_5"/>
    <dbReference type="OMA" id="PHNFQLI"/>
    <dbReference type="OrthoDB" id="784095at2"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990107">
        <property type="term" value="F:thiazole synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04728">
        <property type="entry name" value="ThiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00443">
        <property type="entry name" value="ThiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033983">
        <property type="entry name" value="Thiazole_synthase_ThiG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008867">
        <property type="entry name" value="ThiG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34266">
        <property type="entry name" value="PTHR34266"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05690">
        <property type="entry name" value="ThiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <keyword id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000236346" description="Thiazole synthase">
        <location>
            <begin position="1"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with DXP" evidence="1">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>1-deoxy-D-xylulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="184"/>
            <end position="185"/>
        </location>
        <ligand>
            <name>1-deoxy-D-xylulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="206"/>
            <end position="207"/>
        </location>
        <ligand>
            <name>1-deoxy-D-xylulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00443"/>
        </source>
    </evidence>
    <sequence length="260" mass="27652" checksum="2275FBE7F4E3DB6F" modified="2005-10-11" version="1">MVSFYDREFSSRLLIGTALYPSPKIMQDAIRAAGSQIVTVSLRRETAGGKTGDAFWSLIRELDVTVLPNTAGCKTVREAVTTAKLARELFGTSWIKLEVIADNDTLQPDVVGLVEAAAILIRDGFDVFPYCTEDLGVAMRLVEAGCKVVMPWAAPIGSAKGITNRDALKLLRERLPDVTLVVDAGLGAPSHAAQACELGYDAVLLNTAVAKAADPVVMAGAFRLAVEAGRNAYEAGLMEARDFASPSTPVVGTPFWHAVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ13</accession>
    <name>Q3SQ13_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2374</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2374"/>
    <dbReference type="EnsemblBacteria" id="ABA05628">
        <property type="protein sequence ID" value="ABA05628"/>
        <property type="gene ID" value="Nwi_2374"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2374"/>
    <dbReference type="HOGENOM" id="CLU_2423960_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="91" mass="10131" checksum="3F087275C557297F" modified="2005-10-11" version="1">MTRGWTGTRFQIVRLVSRTDWSLVSNDGAFYLGESAIATHVVRDPASRTAFFVQGRSIAFSSEVETGSREENASKQDHRASLLILSEAKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ73</accession>
    <name>Q3SQ73_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome-c oxidase</fullName>
            <ecNumber evidence="4">7.1.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Nwi_2314</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>4 Fe(II)-[cytochrome c] + 8 H(+)(in) + O2 = 4 Fe(III)-[cytochrome c] + 4 H(+)(out) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11436"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="7.1.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the heme-copper respiratory oxidase family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.9" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315533.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2314"/>
    <dbReference type="EnsemblBacteria" id="ABA05568">
        <property type="protein sequence ID" value="ABA05568"/>
        <property type="gene ID" value="Nwi_2314"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2314"/>
    <dbReference type="eggNOG" id="COG0843">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011899_6_1_5"/>
    <dbReference type="OMA" id="TIMMFLF"/>
    <dbReference type="OrthoDB" id="316745at2"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070469">
        <property type="term" value="C:respirasome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006119">
        <property type="term" value="P:oxidative phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.210.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023616">
        <property type="entry name" value="Cyt_c_oxase-like_su1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036927">
        <property type="entry name" value="Cyt_c_oxase-like_su1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000883">
        <property type="entry name" value="Cyt_C_Oxase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023615">
        <property type="entry name" value="Cyt_c_Oxase_su1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000298">
        <property type="entry name" value="Cyt_c_oxidase-like_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014241">
        <property type="entry name" value="Cyt_c_oxidase_su1_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035973">
        <property type="entry name" value="Cyt_c_oxidase_su3-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013833">
        <property type="entry name" value="Cyt_c_oxidase_su3_a-hlx"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422">
        <property type="entry name" value="PTHR10422"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00115">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01165">
        <property type="entry name" value="CYCOXIDASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81442">
        <property type="entry name" value="SSF81442"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81452">
        <property type="entry name" value="SSF81452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02891">
        <property type="entry name" value="CtaD_CoxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50855">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00077">
        <property type="entry name" value="COX1_CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50253">
        <property type="entry name" value="COX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9" id="KW-0349">Heme</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="78"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="165"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="203"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="263"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="289"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="320"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="358"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="396"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="428"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="470"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="586"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="610"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="657"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="697"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="727"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="769"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="813"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="domain" description="COX1" evidence="12">
        <location>
            <begin position="13"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="COX3" evidence="11">
        <location>
            <begin position="575"/>
            <end position="830"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA05568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="839" mass="92667" checksum="5F015410F1B95946" modified="2005-10-11" version="1">MSGRVATPLRLHRQLNEIWASGRGVKRLAAVNHSVIGTRFMITAFIFFGIGGLLAMLIRAQLATSRSAFIGPDLYNQLFTMHGTIMMFLFAIPMFEGMAIYLLPKLLGTRDLAFPRLGAFGYWCYLFGGAILIAAMLAGAAPDSGWFMYTPLSSSAFTPGINADVWLLGITFVEISAVTAAVELVVSILKIRAPGMSLSRMPIFGWYVLVTAGMMVLGFPPLILGSVLLELERAFDLPFFDTARGGDALLWQHLFWLFGHPEVYIIFLPAAGAISTILPVFARRKLIGYRAVVVALVALAFLSFGLWVHHMFAVGIPHMALAFFSAASAAVTVPTAVQIFAWIATLAHGRPRFDIPMLWIFGFLFVFILGGLTGVMLAMVPFNWQAHDTQFVTAHLHYVLVGGFIFPLVAALYYWLPHICGRIPAFRLSMPAFWLVFIGFNVTFFMMHLTGLRGMPRRVDTYPVEAGWDWLNLTSSIGGFILTMGFALIIVDVFLQIRYGRRFRRNPWKARTLEWAQPIPPPAYNFLSLPWIERRADTLEPESIGPQLAAGHGYLGFARNGWMETLGVDITSGRTDHVIVLPRQTYLPLWTALAVATFVLSLLFSYYWFSLLPLGVAFMLLLCWTRVTGLDRDNGPVVIGREEVAPLHYETHRPPSWWGMIITLIADAAFYGSLVFSPLFLWLTAPAWPPPQTLKSGLVVPALLVAALAAAPLCARRAVARNARSRSPVLWLGATAVLQFASLILLVILMRTSLPAPTAHAYNATSFALLAYSALHTFLATVFCLFGLRRVLTGYVSARRSLDLRIATPLHDYTAITGFAGIGLVLLLPALITAGEAFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQB9</accession>
    <name>Q3SQB9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PNP_UDP_1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2268</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2268"/>
    <dbReference type="EnsemblBacteria" id="ABA05522">
        <property type="protein sequence ID" value="ABA05522"/>
        <property type="gene ID" value="Nwi_2268"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2268"/>
    <dbReference type="eggNOG" id="COG0775">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031248_4_0_5"/>
    <dbReference type="OMA" id="VDMESHV"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009116">
        <property type="term" value="P:nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017831">
        <property type="entry name" value="Hopanoid-assoc_phosphoryl_HpnG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="SSF53167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03468">
        <property type="entry name" value="HpnG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNP_UDP_1" evidence="2">
        <location>
            <begin position="44"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="276" mass="28321" checksum="EEFCF13A52AFEECD" modified="2005-10-11" version="1">MSVGDALDPRPVLIVTGLVQEARIAAGPGMTVICSSSNPRQLRKLLDQFDSSTVRGVISFGVAGGLDPSLKSGDVVVATEVVNGPSRWRAGLALSEELIDGTGLGGQKVVRAGLAGVEQVVLGQAGKAVLHATTGASAVDMESHIAAAYATEAGLPFAALRVISDPATRALPAIARNAIKPNGNIDLKKVLSGLARNPLTLRALVSTGVDFNRALRSLRGCRVFLLGGATLAPADYPAAPAARSRGPGHRKGRAGEPVPARRFSGLRASVPQPARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQC2</accession>
    <name>Q3SQC2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_2265</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2265"/>
    <dbReference type="EnsemblBacteria" id="ABA05519">
        <property type="protein sequence ID" value="ABA05519"/>
        <property type="gene ID" value="Nwi_2265"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2265"/>
    <dbReference type="eggNOG" id="COG4258">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009099_0_0_5"/>
    <dbReference type="OMA" id="AFKIYYV"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017841">
        <property type="entry name" value="Hopanoid_biosynth_HpnN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004869">
        <property type="entry name" value="MMPL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03176">
        <property type="entry name" value="MMPL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03480">
        <property type="entry name" value="HpnN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="299"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="323"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="351"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="428"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="477"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="738"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="765"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="795"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="827"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="859"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="7">
        <location>
            <begin position="324"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA05519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="887" mass="95283" checksum="9EABF111DA0E3964" modified="2005-10-11" version="1">MKRTRQNKRRSARGAYPYTVAERKNVLTSTVVSIVKACTRFAWATALIATVLAAGAVFYSVKNFEINTDINRLISPDLDWRQRDIAFEKAFDRERLILAVVEAPTAEFASAAGAMLAAKLTEDHRHFESVQFLGGGPFFERNGLLFLPTEEVKQLTGQFGEAAPLVEIMAGDPSLRGLTAALETALVGLKRGQFELDSTARPFSLIAGSVEDVLTKGSGVFSWRQLASDKPLPDSDRRSLIQIKPYLDFNALEPGKDATDAIRNAASDLDIAGKFGARVRLTGPVPIANEEFATVQEGAIVNGVGTVLIVLVILWLALRSPKIILAVSATLFIGLAITTAVGLMMVTSLTLLSIAFAVLFVGLGVDFGIQYSVRYRSERFKSDDLERALVVAAKRSSVPLSLAALATAAGFLSFMPTDYKGVSELGKIAGVGMMIAFFASITILPALLRILNPPGEKEPVGYAFLAPLDRFLEDHRVAIVGGTIAVVILGAPALYFLRFDFNPIHLRSAKVESIATYLDLRKDPDTGANAINVMVKSEQEARQVEARLAEVPEVASTRSLDSFVPADQPAKLKLIARGAKILDPALNPDSIDPPPSDEENIDALKGTADSLRKAAGTHQGAGATAAKRLADDLTRLADSNQATREKAQAAFVEPLKIMFDQLRNLLKAEPVTLESLPPDFVNLWKSEDGITRVEVLPRGDPDNNETLREFASAVLAAAPNAIGGPVSILKSGDTIVKAFIEAGLWALVSISILLWLALRRITDVLLTLVPLLVAGAVTLEICVLIGLPLNFANIVALPLLLGVGVAFKIYYVTAWRSGRTNLLQSSLTRAIFFSALTTATAFGSLWLSSHPGTASMGKLLALSLITTLAAVLLFQPALMGRPRDVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQE9</accession>
    <name>Q3SQE9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Heterodimeric methylmalonyl-CoA mutase large subunit</fullName>
            <ecNumber evidence="7">5.4.99.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2238</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="5.4.99.2" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2238"/>
    <dbReference type="EnsemblBacteria" id="ABA05492">
        <property type="protein sequence ID" value="ABA05492"/>
        <property type="gene ID" value="Nwi_2238"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2238"/>
    <dbReference type="eggNOG" id="COG1884">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2185">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009523_3_1_5"/>
    <dbReference type="OMA" id="PWGGSAY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004494">
        <property type="term" value="F:methylmalonyl-CoA mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006159">
        <property type="entry name" value="Acid_CoA_mut_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016176">
        <property type="entry name" value="Cbl-dep_enz_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036724">
        <property type="entry name" value="Cobalamin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006099">
        <property type="entry name" value="MeMalonylCoA_mutase_a/b_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006098">
        <property type="entry name" value="MMCoA_mutase_a_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01642">
        <property type="entry name" value="MM_CoA_mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51703">
        <property type="entry name" value="SSF51703"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52242">
        <property type="entry name" value="SSF52242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00640">
        <property type="entry name" value="acid_CoA_mut_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00641">
        <property type="entry name" value="acid_CoA_mut_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00544">
        <property type="entry name" value="METMALONYL_COA_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0846">Cobalamin</keyword>
    <keyword evidence="5" id="KW-0170">Cobalt</keyword>
    <keyword evidence="4 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B12-binding" evidence="6">
        <location>
            <begin position="586"/>
            <end position="718"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="722" mass="78837" checksum="D25F5575E8EAB8E8" modified="2005-10-11" version="1">MMARVPDFADVAFEPVAPPVAGGFERSPEVWLTPEGIPVKTAYSEADLDGIDFLDTWPGIAPYLRGPYPTMYVNQPWTIRQYAGFSTAEDSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVSGDVGMAGVAIDSIYDMRTLFAGIPLDRISVSMTMNGAVLPILALFVAAAEEQGVPAEKLSGTIQNDILKEFMVRNTYIYPPAPSMRIVSDIFAYASRRMPKFNSISVSGYHMQEAGATQDLELAYTLADGVEYVRAGLKAGLDIDRFAPRLSFFWATGMNFFMEVAKMRAARLLWAKLMKPFNPKNPRSLSLRTHCQTSGWSLTAQDVFNNVIRTAIEAMAATQGHTQSLHTNALDEALALPTDFSARIARNTQLFLQQESGTTRIIDPWGGSHYVERLTHDLAIKAWGHIREVEELGGMARAIEAGVPKLRIEEAAARTQARIDAGAQVVIGVNRYKPEHETPIDVLRIDNSAVRSLQIEKLKRLRGERRQEDVDAALAALTRSAGEGNGNLLALAIDAARAKATVGEISDAMEKVFGRHRAEIRSITGVYKREVSAMSDKVEQLQRLIDAFDEAEGRRPRILVAKIGQDGHDRGQKVIASAFADIGFDVDIGPLFATAEEAARQAIENDVHILGVSSLAAAHLTAVPELKAALTRHGRDDIMIIVGGVVPPQDYDALYKAGAEAIFPPGTVIADAAEDLIRKLSARLGHGTTME</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQP2</accession>
    <name>Q3SQP2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2145</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2145"/>
    <dbReference type="EnsemblBacteria" id="ABA05399">
        <property type="protein sequence ID" value="ABA05399"/>
        <property type="gene ID" value="Nwi_2145"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2145"/>
    <dbReference type="eggNOG" id="ENOG5032M5C">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1336340_0_0_5"/>
    <dbReference type="OMA" id="DAIRDNH"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="218" mass="23997" checksum="DB66070FAEE4A064" modified="2005-10-11" version="1">MASRAITREHENMVDWTDDRIGALSDSGLKNLLANAERKSVDDLVARCRAELEKRNVSKPRKASSKARSGLKDFEHEMSLQLAVVGRRMAEKYDLSEETAKVKSAGVKGFRAHKLVGSDGHAKLGGLQRAGFVAIDRYISYRRCSDIVSLGVFLPKDQDISEHRFFVIAPQEVLDGGKPVDAIRDNHGQKQSADSALMFDDLEAAADAFDKVLARIAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQT7</accession>
    <name>Q3SQT7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Type IV secretion system protein VirB4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2097</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2097"/>
    <dbReference type="EnsemblBacteria" id="ABA05354">
        <property type="protein sequence ID" value="ABA05354"/>
        <property type="gene ID" value="Nwi_2097"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2097"/>
    <dbReference type="eggNOG" id="COG3451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118466_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="156" mass="17169" checksum="836FB7F528533109" modified="2005-10-11" version="1">MFSSQSLADIEASPIAPVLVESCPTRIFLANERAIEPQITAVYERFGLNARQIEIIARATLKRDYYCQSRRGNRLFELGLGSVALALCASSDKAAHALIDELLRDGARPYFLEAWLYANDLAWAGDLVPNLTNVIDSSATADRPVSIDPKEQETPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR10</accession>
    <name>Q3SR10_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TPR_21 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2022</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315267.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2022"/>
    <dbReference type="EnsemblBacteria" id="ABA05281">
        <property type="protein sequence ID" value="ABA05281"/>
        <property type="gene ID" value="Nwi_2022"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2022"/>
    <dbReference type="eggNOG" id="COG4649">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073302_1_1_5"/>
    <dbReference type="OMA" id="EQNKHAE"/>
    <dbReference type="OrthoDB" id="1926596at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018704">
        <property type="entry name" value="TPR_21"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09976">
        <property type="entry name" value="TPR_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="TPR_21" evidence="3">
        <location>
            <begin position="18"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="217" mass="23676" checksum="6D6B455908824739" modified="2005-10-11" version="1">MTEIYSEIDDELRRERLKKIWGQYSALIIAAVILVLVAVGGWRGYQYLQTQKAAEASSAFEAAAALADQNKHAEAQAAFAKLAETAPLGYRNLSRLRAAAEAARHDINAAVKLYDAIATEGGFSSVERDFARVRAAALLVDSETYNNMLQRLEPAAAPEATFRHSARELLALSAWRSDDATAARKWLDMISEDSQTPSSLRGRAEALQALLPPVAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR22</accession>
    <name>Q3SR22_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SMF protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2010</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DprA/Smf family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315255.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2010"/>
    <dbReference type="EnsemblBacteria" id="ABA05269">
        <property type="protein sequence ID" value="ABA05269"/>
        <property type="gene ID" value="Nwi_2010"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2010"/>
    <dbReference type="eggNOG" id="COG0758">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029601_1_1_5"/>
    <dbReference type="OMA" id="SEMPFGW"/>
    <dbReference type="OrthoDB" id="1881437at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009294">
        <property type="term" value="P:DNA-mediated transformation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003488">
        <property type="entry name" value="DprA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041614">
        <property type="entry name" value="DprA_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43022">
        <property type="entry name" value="PTHR43022"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02481">
        <property type="entry name" value="DNA_processg_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17782">
        <property type="entry name" value="DprA_WH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00732">
        <property type="entry name" value="dprA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DprA_WH" evidence="2">
        <location>
            <begin position="312"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="372" mass="39461" checksum="991FC2ABA46DC2F2" modified="2005-10-11" version="1">MNIRDPDRRLSEAQRTDWLRLIRSDNVGPRTFRSLVNHFGSASEALVRLPDLARRGGASRPSRLCTEAEARNELAAARRIGVSLLAPGEAGYPPHLATIDDAPPLLGARGNLDVMERPMIAIVGSRNASGAGHKFAQTLAHDLGDTGFVIVSGLARGIDQAAHRASVARGTVAVLAGGHDRIYPLEHEDLLAAVLESGGAISEMPMGHVPRARDFPRRNRLISGAAIGVVVVEAAHRSGSLITARMAAEQGREVFAVPGSPLDPRATGTNDLIKQGATLITEAADVINAVRPIIRRPVDLPAEEPEPGEPWTEEPAASDRARIIALLGPAPIGLDDLIRMANAPPAVVRTVLLELELAGRLERHGGGLVSMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR37</accession>
    <name>Q3SR37_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1995</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315241.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1995"/>
    <dbReference type="EnsemblBacteria" id="ABA05254">
        <property type="protein sequence ID" value="ABA05254"/>
        <property type="gene ID" value="Nwi_1995"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1995"/>
    <dbReference type="eggNOG" id="COG0790">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_146682_1_0_5"/>
    <dbReference type="OMA" id="YATGRGC"/>
    <dbReference type="OrthoDB" id="1915444at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="88" mass="9615" checksum="738F668FE3BCC8EC" modified="2005-10-11" version="1">MFQGQIDFETAVPVSADAVQDILFERGLYWASGRAGLVDLVAAHKWFNLAALKGHAEAAAMRREVAALMSGAEIVMAQRDARAWMTVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR38</accession>
    <name>Q3SR38_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1994</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05253">
        <property type="protein sequence ID" value="ABA05253"/>
        <property type="gene ID" value="Nwi_1994"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1994"/>
    <dbReference type="HOGENOM" id="CLU_2423959_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="91" mass="10359" checksum="8EB112A029C59552" modified="2005-10-11" version="1">MCDDASHHLKLARPLQSLTTKGHRTQTARKLPRYVTRPRIMSVLDVYVYVIPFLVISATIGAVLWNRRKINRARASDINKKPEDESLSASD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="130" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR82</accession>
    <name>Q3SR82_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Heavy metal translocating P-type ATPase</fullName>
            <ecNumber evidence="8">3.6.3.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_1949</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.3" evidence="8"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1949"/>
    <dbReference type="EnsemblBacteria" id="ABA05209">
        <property type="protein sequence ID" value="ABA05209"/>
        <property type="gene ID" value="Nwi_1949"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1949"/>
    <dbReference type="eggNOG" id="COG2217">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001771_6_3_5"/>
    <dbReference type="OMA" id="HLHLMET"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00941">
        <property type="entry name" value="CDATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="SSF81653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="76"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="243"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="273"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="583"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="606"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="631"/>
            <end position="665"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA05209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="665" mass="69496" checksum="5ABF9107C5285DA4" modified="2005-10-11" version="1">MSKLFNLDRAMILTALTVAGMLLAILGLLPLQGPAAGFFSRTGLSLVYLAGGLPAAWRAAVALWEERILDIDLLMVVAAVAAAAVGAPFEGAVLLTLFSISTTLEERALGRARRAIEALMELRPETALRKAPDGSVSEVPAADLKVDDTVVLRPGARVPADGVIVGGRGSLDEANITGESMPVAKQPGAQVFEATVNLDGVLEVAVTKTVEESTVARMIALVTEAQAAKAPSERFSAWFGQRYTVAVMAGAVLAFAVFYWLGRDWEEALYRSATLLVAASPCAIVISVPAAILSALSAAARGGVLFKGGAALETLAAVDIFAFDKTGTLTTGKASVTGVVALDGDERLFLSLLAGLEAHSEHHSAAAIRQEAASRGIAPIRVMNVITRPSAGIVGDDDKGQLWAGNPRLAVQMGAPIDHPMLKALAADTQTVVYVGRDSRVMGAVTIADQARPTSAPALAALRESGVSQIVMMTGDRRPVALRIGAELGLKPEEIYADMLPEDKVRMAGELATTTRGMVAFVGDGVNDAAALARADVGIAMGAAGSDVALQAADVALLSEDMGRLADAHRLARRAATIIRQNLVFAMGVMAILVTGGLFFELPLPLAVIGHEGGTVLVVLNGLRLLRDPIRRGETKPAPKRSVHASSSQPIPRQAYRRKVPRRAN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-21" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRB1</accession>
    <name>ACPS_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Holo-[acyl-carrier-protein] synthase</fullName>
            <shortName evidence="1">Holo-ACP synthase</shortName>
            <ecNumber evidence="1">2.7.8.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">4'-phosphopantetheinyl transferase AcpS</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">acpS</name>
        <name type="ordered locus">Nwi_1920</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of acyl-carrier-protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>apo-[ACP] + CoA = adenosine 3',5'-bisphosphate + H(+) + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:12068"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9690"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="EC" id="2.7.8.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the P-Pant transferase superfamily. AcpS family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.7" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315176.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRB1"/>
    <dbReference type="SMR" id="Q3SRB1"/>
    <dbReference type="STRING" id="323098.Nwi_1920"/>
    <dbReference type="EnsemblBacteria" id="ABA05180">
        <property type="protein sequence ID" value="ABA05180"/>
        <property type="gene ID" value="Nwi_1920"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1920"/>
    <dbReference type="eggNOG" id="COG0736">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089696_0_2_5"/>
    <dbReference type="OMA" id="DERHYAV"/>
    <dbReference type="OrthoDB" id="1893660at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008897">
        <property type="term" value="F:holo-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00101">
        <property type="entry name" value="AcpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008278">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037143">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002582">
        <property type="entry name" value="ACPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004568">
        <property type="entry name" value="Ppantetheine-prot_Trfase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01648">
        <property type="entry name" value="ACPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56214">
        <property type="entry name" value="SSF56214"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00516">
        <property type="entry name" value="acpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00556">
        <property type="entry name" value="pantethn_trn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword id="KW-0276">Fatty acid metabolism</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000228293" description="Holo-[acyl-carrier-protein] synthase">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00101"/>
        </source>
    </evidence>
    <sequence length="139" mass="15275" checksum="84D68773AAB429AA" modified="2005-10-11" version="1">MIIGIGSDLIDIRRVAKVIERHGDRFLNRVFTEIERAKAERRATNEKMVVSTYAKRFAAKEACAKALGTGIRQGVWWRDMGVVNLQAGQPSMMLTGGALKRLQLLTPPGLEVRIDVSLTDDWPLAQAFVIISAAAAGKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRD3</accession>
    <name>CLPX_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent Clp protease ATP-binding subunit ClpX</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">clpX</name>
        <name type="ordered locus">Nwi_1898</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of ClpP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the ClpX-ClpP complex. Forms a hexameric ring that, in the presence of ATP, binds to fourteen ClpP subunits assembled into a disk-like structure with a central cavity, resembling the structure of eukaryotic proteasomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ClpX chaperone family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315154.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRD3"/>
    <dbReference type="SMR" id="Q3SRD3"/>
    <dbReference type="STRING" id="323098.Nwi_1898"/>
    <dbReference type="EnsemblBacteria" id="ABA05158">
        <property type="protein sequence ID" value="ABA05158"/>
        <property type="gene ID" value="Nwi_1898"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1898"/>
    <dbReference type="eggNOG" id="COG1219">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014218_8_2_5"/>
    <dbReference type="OMA" id="HYKRVQA"/>
    <dbReference type="OrthoDB" id="718259at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00175">
        <property type="entry name" value="ClpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004487">
        <property type="entry name" value="Clp_protease_ATP-bd_su_ClpX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046425">
        <property type="entry name" value="ClpX_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010603">
        <property type="entry name" value="Znf_CppX_C4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038366">
        <property type="entry name" value="Znf_CppX_C4_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06689">
        <property type="entry name" value="zf-C4_ClpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00994">
        <property type="entry name" value="zf-C4_ClpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00382">
        <property type="entry name" value="clpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51902">
        <property type="entry name" value="CLPX_ZB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_1000024600" description="ATP-dependent Clp protease ATP-binding subunit ClpX">
        <location>
            <begin position="1"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="ClpX-type ZB" evidence="2">
        <location>
            <begin position="3"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="119"/>
            <end position="126"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01250"/>
        </source>
    </evidence>
    <sequence length="424" mass="46580" checksum="28A24B1E937278F7" modified="2005-10-11" version="1">MSKVGTGDSKNTLYCSFCGKSQHEVRKLIAGPTVFICDECVELCMDIIREENKSSLVKSRDGIPTPKEICKVLDDYVIGQGHAKKVLSVAVHNHYKRLNHQTKHNDVELAKSNILLIGPTGSGKTLLAQTLARILDVPFTMADATTLTEAGYVGEDVENIILKLLQSADYNVERAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKIMEGTVASVPPQGGRKHPQQEFLQVDTTNILFICGGAFSGLEKIISARGRTTSIGFAAQVLAPEDRRTGEIFRHVEPEDLLKYGLIPEFVGRLPVVATLEDLDEASLKKILTDPKNALVKQYQRLFEMENIELTFADEALGAVARKAIERKTGARGLRSILESILLETMFDLPGLEGVEEVVISREVVEGTARPLYIYADRADRASETSASA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRG9</accession>
    <name>PYRF_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Orotidine 5'-phosphate decarboxylase</fullName>
            <ecNumber evidence="1">4.1.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">OMP decarboxylase</fullName>
            <shortName evidence="1">OMPDCase</shortName>
            <shortName evidence="1">OMPdecase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrF</name>
        <name type="ordered locus">Nwi_1862</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the decarboxylation of orotidine 5'-monophosphate (OMP) to uridine 5'-monophosphate (UMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + orotidine 5'-phosphate = CO2 + UMP</text>
            <dbReference type="Rhea" id="RHEA:11596"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="EC" id="4.1.1.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the OMP decarboxylase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.23" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315118.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRG9"/>
    <dbReference type="SMR" id="Q3SRG9"/>
    <dbReference type="STRING" id="323098.Nwi_1862"/>
    <dbReference type="EnsemblBacteria" id="ABA05122">
        <property type="protein sequence ID" value="ABA05122"/>
        <property type="gene ID" value="Nwi_1862"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1862"/>
    <dbReference type="eggNOG" id="COG0284">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067069_1_0_5"/>
    <dbReference type="OMA" id="NFKIFLD"/>
    <dbReference type="OrthoDB" id="1150446at2"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00120"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004590">
        <property type="term" value="F:orotidine-5'-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01200_B">
        <property type="entry name" value="OMPdecase_type1_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014732">
        <property type="entry name" value="OMPdecase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001754">
        <property type="entry name" value="OMPdeCOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32119:SF2">
        <property type="entry name" value="PTHR32119:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00215">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00934">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01740">
        <property type="entry name" value="pyrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0210">Decarboxylase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000241879" description="Orotidine 5'-phosphate decarboxylase">
        <location>
            <begin position="1"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01200"/>
        </source>
    </evidence>
    <sequence length="240" mass="26061" checksum="2860876D5CA750B1" modified="2005-10-11" version="1">MTGSHDSDLAREKLIVALDYWNIEDARKLVRDLGDGVSFYKVGLGLQLVGGNEFARELIAEGKRVFLDYKYYDIEETVQRAVAQAAELKIAFLTVHGVTSIMKAAVAGRGNSDMKILGVTVLTSMDAEDIKEMGFECSVEDLVVARARRALEVGVDGVVASALEAAELRKHTNNKLMIVSPGIRPSGGARHDQKRVATPFEAMRAGADYLVLGRPIYAADNPKAAAQAIIREMADALRPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRM4</accession>
    <name>Q3SRM4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Proline-rich protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1806</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1806"/>
    <dbReference type="EnsemblBacteria" id="ABA05067">
        <property type="protein sequence ID" value="ABA05067"/>
        <property type="gene ID" value="Nwi_1806"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1806"/>
    <dbReference type="eggNOG" id="COG3115">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041662_0_0_5"/>
    <dbReference type="OMA" id="RAMVGPF"/>
    <dbReference type="OrthoDB" id="933232at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042834">
        <property type="term" value="F:peptidoglycan binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007730">
        <property type="entry name" value="SPOR-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036680">
        <property type="entry name" value="SPOR-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05036">
        <property type="entry name" value="SPOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110997">
        <property type="entry name" value="SSF110997"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51724">
        <property type="entry name" value="SPOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="SPOR" evidence="3">
        <location>
            <begin position="385"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="290"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="470" mass="50090" checksum="06E237DFA78D5D9F" modified="2005-10-11" version="1">MAHRYQDRPFPEDDDYGRDDQRATAPTEPDPLAELARLIGQGDSFGEPRRETPMAPVDDPVDHFEPLPPIEEPIEGEPPAGPPSWIQRRMARPDPSPEQLESSAHPVLRRAAVYPDSHQPEPESYQADPGRYDDARYGNLPDDQPAYAYGDRVPHDAYEGQYPRETYPEAPFGDENGYGESYGYGEAEDEPAPGRRGKMATVVAVLALAVVGTGAAYAYRTFVGNAPNGEPPIIRADTEPNKIVPQQTTSGDAVGKLLQDRMSVENGTEQVVSREEQPVDVKSAVSAGPRVVFPPLNQNDNPPSAASVAPDIKPPATVANSALGGDEPRKVRTLAVRGDQADLAAASAASRHAAAAGAAPGANVPLSQTADTRTRMASTHPAQQSSASGGYLVQVSSQRNEADAQASFRVLQGKFPSMLGSRTPVIKRADLGAKGVYYRAMVGPFASSGEASRFCGSLKSAGGQCVVQRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRN7</accession>
    <name>Q3SRN7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1793</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315050.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05054">
        <property type="protein sequence ID" value="ABA05054"/>
        <property type="gene ID" value="Nwi_1793"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1793"/>
    <dbReference type="eggNOG" id="ENOG50316DE">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118465_0_0_5"/>
    <dbReference type="OMA" id="RADSQFR"/>
    <dbReference type="OrthoDB" id="1707166at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229172" evidence="1">
        <location>
            <begin position="31"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="165" mass="17045" checksum="6F4C1691EC776920" modified="2005-10-11" version="1">MGFGGIMSRGLSAACALGAVVMLSMSTSLAGAGPFEGLAGIWTGRGTIQLEDGSTEKIRCKATYAVSGDAQGLNQTLLCASDSYKFELKSNVLARNGVLSGDWRETSRNVGGTLEGRAGNGQFNVDVSAPAFTAKLKLKTNGNRQDVVISSDGQFKGASISLSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRT9</accession>
    <name>Q3SRT9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1741</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1741"/>
    <dbReference type="EnsemblBacteria" id="ABA05002">
        <property type="protein sequence ID" value="ABA05002"/>
        <property type="gene ID" value="Nwi_1741"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1741"/>
    <dbReference type="HOGENOM" id="CLU_2330896_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="98" mass="10879" checksum="17F0773FD7643100" modified="2005-10-11" version="1">MKKTRRVEAAQWRSGCGIHFTRKRYNVLAAFAMLSCPRFARLINETSNRTKDPLSSSLDCADPGLSGRPADACRAPARFSDRTGGHVPLFAPERATER</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRV0</accession>
    <name>Q3SRV0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Surface presentation of antigens (SPOA) protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1730</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FliN/MopA/SpaO family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1730"/>
    <dbReference type="EnsemblBacteria" id="ABA04991">
        <property type="protein sequence ID" value="ABA04991"/>
        <property type="gene ID" value="Nwi_1730"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1730"/>
    <dbReference type="eggNOG" id="COG1886">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097058_4_3_5"/>
    <dbReference type="OMA" id="KQMLPRT"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001543">
        <property type="entry name" value="FliN-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001172">
        <property type="entry name" value="FliN_T3SS_HrcQb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036429">
        <property type="entry name" value="SpoA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01052">
        <property type="entry name" value="FliMN_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00956">
        <property type="entry name" value="FLGMOTORFLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101801">
        <property type="entry name" value="SSF101801"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FliMN_C" evidence="2">
        <location>
            <begin position="21"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="97" mass="10644" checksum="D9945BF0DCDF9C01" modified="2005-10-11" version="1">MWWCGTVAIGMAPGLNFVATLDKVSVDLMVVLGTTSMPVHQVMRLSRGAIIELDATEQDEVKILANNLPVASGVVMVNRNRITVEVKQMLPRAPDRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRV5</accession>
    <name>Q3SRV5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Riboflavin biosynthesis protein RibD</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="10">Diaminohydroxyphosphoribosylaminopyrimidine deaminase</fullName>
                <shortName evidence="10">DRAP deaminase</shortName>
                <ecNumber evidence="10">3.5.4.26</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="10">Riboflavin-specific deaminase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="10">5-amino-6-(5-phosphoribosylamino)uracil reductase</fullName>
                <ecNumber evidence="10">1.1.1.193</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="10">HTP reductase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">Nwi_1725</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Converts 2,5-diamino-6-(ribosylamino)-4(3h)-pyrimidinone 5'-phosphate into 5-amino-6-(ribosylamino)-2,4(1h,3h)-pyrimidinedione 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + H(+) + H2O = 5-amino-6-(5-phospho-D-ribosylamino)uracil + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:21868"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:58453"/>
            <dbReference type="ChEBI" id="CHEBI:58614"/>
            <dbReference type="EC" id="3.5.4.26"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>5-amino-6-(5-phospho-D-ribitylamino)uracil + NADP(+) = 5-amino-6-(5-phospho-D-ribosylamino)uracil + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:17845"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58421"/>
            <dbReference type="ChEBI" id="CHEBI:58453"/>
            <dbReference type="EC" id="1.1.1.193"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10 13">Binds 1 zinc ion.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 3/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 10">In the C-terminal section; belongs to the HTP reductase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">In the N-terminal section; belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.26" evidence="10"/>
    <dbReference type="EC" id="1.1.1.193" evidence="10"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314982.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1725"/>
    <dbReference type="EnsemblBacteria" id="ABA04986">
        <property type="protein sequence ID" value="ABA04986"/>
        <property type="gene ID" value="Nwi_1725"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1725"/>
    <dbReference type="eggNOG" id="COG0117">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1985">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036590_1_1_5"/>
    <dbReference type="OMA" id="PYIILKW"/>
    <dbReference type="OrthoDB" id="1775351at2"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00401"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008703">
        <property type="term" value="F:5-amino-6-(5-phosphoribosylamino)uracil reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008835">
        <property type="term" value="F:diaminohydroxyphosphoribosylaminopyrimidine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004794">
        <property type="entry name" value="Eubact_RibD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002734">
        <property type="entry name" value="RibDG_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01872">
        <property type="entry name" value="RibD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006769">
        <property type="entry name" value="RibD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="SSF53597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00326">
        <property type="entry name" value="eubact_ribD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="10 12" id="KW-0521">NADP</keyword>
    <keyword evidence="10 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="8 10 13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="14">
        <location>
            <begin position="19"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="317"/>
            <end position="323"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR006769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR006769-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR006769-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR006769-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABA04986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="387" mass="40115" checksum="7BB163BD5F4B5D63" modified="2005-10-11" version="1">MIVGVLEEEHGASLRTERTVDLHFMQLALALGRRSLGASAPNPAVGAVIVKDGVIVGRGWTQPGGRPHAEAEALRRAGEAARGATLYVTLEPCSHVGKTPPCADAVIAAGITRVVSAIEDPTPEVSGQGHARLRAAGVAVEIGLCAAEAAHHHAGHFRRVRDRRPHIILKLAVSSDGKIAAAGGVPVAITGEAARARAHLLRAQCSAILVGIGTVLADDPLLTCRLPGMETRSPLRVVLDQALRIPESSRLVQSARETPLWIMTSEFAEASRAMKLGAAGARVMRVPGAAGAPGLDLVAVLRALSESGVTRLVVEGGARVAASFIAAALVDEAWLLRGPDPIGDDGVAALGALPLTVITGSPEFRMCAGETLDQDALAIYERVRSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRY5</accession>
    <name>Q3SRY5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">AAA ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_1695</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the ClpA/ClpB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314954.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1695"/>
    <dbReference type="EnsemblBacteria" id="ABA04956">
        <property type="protein sequence ID" value="ABA04956"/>
        <property type="gene ID" value="Nwi_1695"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1695"/>
    <dbReference type="eggNOG" id="COG0542">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005070_4_2_5"/>
    <dbReference type="OMA" id="GRVIQEH"/>
    <dbReference type="OrthoDB" id="44062at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043335">
        <property type="term" value="P:protein unfolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1780.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036628">
        <property type="entry name" value="Clp_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004176">
        <property type="entry name" value="Clp_R_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013461">
        <property type="entry name" value="ClpA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018368">
        <property type="entry name" value="ClpA/B_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028299">
        <property type="entry name" value="ClpA/B_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041546">
        <property type="entry name" value="ClpA/ClpB_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17871">
        <property type="entry name" value="AAA_lid_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02861">
        <property type="entry name" value="Clp_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81923">
        <property type="entry name" value="SSF81923"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02639">
        <property type="entry name" value="ClpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51903">
        <property type="entry name" value="CLP_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00870">
        <property type="entry name" value="CLPAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00871">
        <property type="entry name" value="CLPAB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Clp R" evidence="9">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="765"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA04956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="801" mass="88274" checksum="FC565D0C04DC58DC" modified="2005-10-11" version="1">MPTFSQSLEQSLHRALAIANERHHQYATLEHLLLSLVDDSDAAAVMRACSVDLDKLRGSLVNYLETEFENLVTAEAADDAKPTAGFQRVIQRAVIHVQSSGREEVTGANVLIAIFAERESHAAYFLQEQDMTRYDAVNYISHGIAKRPGVSEARPVRGVDEETETKSGDDAKKKGDALETYCVNLNKKARDGKIDPVVGRNTEINRAIQVLCRRQKNNPLFVGDAGVGKTAIAEGLAKRIVDSEVPEVLAAATIFSLDMGTLLAGTRYRGDFEERLKQVLKELEAHPNAVLFIDEIHTVIGAGATSGGAMDASNLLKPALASGTIRCMGSTTYKEYRQHFEKDRALVRRFQKIDVNEPSVEDAIAILKGLKPYFEDYHKLKYTNDAIEAAVQLSSRYIHDRKLPDKAIDVIDESGAAQMLVTENKRKKTIGIKEIETTIAAMARIPPKSMSKDDAEVLKHLEQTLKRVVFGQDRAIEALTASIKLARAGLREPEKPIGSYLFSGPTGVGKTEVAKQLAEALGVELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDGVDQHPHSVVLLDEIEKAHPDVYNVLLQIMDHGRLTDHHGKQVNFRNVILIMTTNAGASDMAKAAFGFTRNKREGDDREAINRQFSPEFRNRLDAVVSFGHLNTDVIGMVVEKFVLQLEAQLADRYVTIELSDTAKAWLVQHGYDEQMGARPMARVIQEHIKKPLADEVLFGKLKGGGHVRVVLVKDEAVAGVDLEKIGFEFIEGPVTPKPENLPGARKRTPPRKPKPGGGGLTSEEPLKV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS04</accession>
    <name>AMPA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable cytosol aminopeptidase</fullName>
            <ecNumber evidence="1">3.4.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Leucine aminopeptidase</fullName>
            <shortName evidence="1">LAP</shortName>
            <ecNumber evidence="1">3.4.11.10</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Leucyl aminopeptidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pepA</name>
        <name type="ordered locus">Nwi_1676</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.</text>
            <dbReference type="EC" id="3.4.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.</text>
            <dbReference type="EC" id="3.4.11.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M17 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.1" evidence="1"/>
    <dbReference type="EC" id="3.4.11.10" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314936.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SS04"/>
    <dbReference type="SMR" id="Q3SS04"/>
    <dbReference type="STRING" id="323098.Nwi_1676"/>
    <dbReference type="EnsemblBacteria" id="ABA04937">
        <property type="protein sequence ID" value="ABA04937"/>
        <property type="gene ID" value="Nwi_1676"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1676"/>
    <dbReference type="eggNOG" id="COG0260">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013734_6_0_5"/>
    <dbReference type="OMA" id="MKNTGPR"/>
    <dbReference type="OrthoDB" id="356206at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00433">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00181">
        <property type="entry name" value="Cytosol_peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011356">
        <property type="entry name" value="Leucine_aapep/pepB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000819">
        <property type="entry name" value="Peptidase_M17_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023042">
        <property type="entry name" value="Peptidase_M17_leu_NH2_pept"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008283">
        <property type="entry name" value="Peptidase_M17_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963">
        <property type="entry name" value="PTHR11963"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00883">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02789">
        <property type="entry name" value="Peptidase_M17_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00481">
        <property type="entry name" value="LAMNOPPTDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="SSF52949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00631">
        <property type="entry name" value="CYTOSOL_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0031">Aminopeptidase</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000019946" description="Probable cytosol aminopeptidase">
        <location>
            <begin position="1"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="276"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="350"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="348"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="348"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00181"/>
        </source>
    </evidence>
    <sequence length="500" mass="52815" checksum="D2ABB61E72182DED" modified="2005-10-11" version="1">MPDAVKVGFVPFSTASRGTLVVFCDETLKFGRAAGKALGSSAGLVKRAAAANQFKGKSATTLDILAPEGLKADRLIVVGVGKTPALKSSDFLKLGGVIAGKVRSDNNGVTIIAELPSATMEPDLSASLASGIRLRAYKFDRYKTRKKEGDDAVNRADVSIAVADVAAAKKAFAPVNHVVNGVIIARDLVNEPPNVLYPEEFARRASRLRAMGVGIDVLDVKAMTKLGMGALLGVGQGSARPSRTVVMRWNGGKKGEPPVAFVGKGVCFDTGGISIKPANGMEEMKGDMGGAACVVGLMHALAARKAKVNAIGAIGIVENMPDGSAQRPGDIVTSMSGQTIEIINTDAEGRLVLADVLWYVARKFKPKVMIDLATLTGAIMVALGTEHAGLFSNNDELSERLTRAGLDTGERVWRMPLAPEYDKLIDSKFADMKNTGGRHGGSITAAQFLQRFVDDTPWAHLDIAGTAMGAPKSDINQSWGSGFGVRLLDRLVAEHYETKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS10</accession>
    <name>Q3SS10_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alkyl hydroperoxide reductase/ Thiol specific antioxidant/ Mal allergen</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1670</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1670"/>
    <dbReference type="EnsemblBacteria" id="ABA04931">
        <property type="protein sequence ID" value="ABA04931"/>
        <property type="gene ID" value="Nwi_1670"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1670"/>
    <dbReference type="eggNOG" id="COG1225">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_14_0_5"/>
    <dbReference type="OMA" id="RVVVYFY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016209">
        <property type="term" value="F:antioxidant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="58"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="209" mass="22161" checksum="B26289D922C9856F" modified="2005-10-11" version="1">MSKKIRKKSSKSDSSVTVRAATVAHTEQAREAGPSPSPAATTKTKPRPAPPASQGAGLAEGSPAPPFNLPREDGALVSLADFAGGKLVIFFYPRAGTPGCTIEAMDFTRLATDFAVSGTAVIGVSADPVKAQLSFRNKHQLSIALISDEKHQMLEAYGVWGEKSMYGKTFMGVLRTTVLVDGAGRIARIWRRVKVEGHADEVLAAAREL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS68</accession>
    <name>Q3SS68_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1612</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04873">
        <property type="protein sequence ID" value="ABA04873"/>
        <property type="gene ID" value="Nwi_1612"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1612"/>
    <dbReference type="eggNOG" id="ENOG5030X5N">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127514_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="125" mass="14670" checksum="ACE0ACDADC402CF0" modified="2005-10-11" version="1">MSNWSAYWRMPSPKQMEKLAREQRQIQTPDGEPDGWWDSVLNDPRATGAPRLPIQQCRLNEIPRDVLRVECFRCMRIVEIEKADAIRLYGPHAIWKDVGRKLLDDGCKHRTGRHEEDGCWPDWTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSA5</accession>
    <name>Q3SSA5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Polyphosphate glucokinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1575</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1575"/>
    <dbReference type="EnsemblBacteria" id="ABA04836">
        <property type="protein sequence ID" value="ABA04836"/>
        <property type="gene ID" value="Nwi_1575"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1575"/>
    <dbReference type="eggNOG" id="COG1940">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065796_1_0_5"/>
    <dbReference type="OMA" id="HVEMLFS"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="236" mass="25827" checksum="1DCAB962891EE69F" modified="2005-10-11" version="1">MKAARSSRMNPTILAIDVGGSNVKMMTNTDRVPRKFESGPGLTAKEMVRKIKALTKDWSFDVVSIGYPGPVCGNRPLREPVNLGVGWKGFDFQKAFGKPTRVVNDALMQALGGYRGGRMLFLGLGTGLGSAMIADGEPEPMELAHLPYRKGKTYEDYAGKAGRKHSGDKKWRKHVFDIIAQLSAALEPDEVLIGGGNARHLEKLPPKSRRGDNADAFEGGFRLWDQSSVDARARVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSF2</accession>
    <name>Q3SSF2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Amino acid ABC transporter membrane protein 1, PAAT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_1528</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the binding-protein-dependent transport system permease family. HisMQ subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314795.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1528"/>
    <dbReference type="EnsemblBacteria" id="ABA04789">
        <property type="protein sequence ID" value="ABA04789"/>
        <property type="gene ID" value="Nwi_1528"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1528"/>
    <dbReference type="eggNOG" id="COG4597">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019602_8_0_5"/>
    <dbReference type="OMA" id="GKPMRVW"/>
    <dbReference type="OrthoDB" id="1523519at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010065">
        <property type="entry name" value="AA_ABC_transptr_permease_3TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043429">
        <property type="entry name" value="ArtM/GltK/GlnP/TcyL/YhdX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30614">
        <property type="entry name" value="PTHR30614"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01726">
        <property type="entry name" value="HEQRo_perm_3TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="90"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="188"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="342"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="366"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="95"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA04789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="401" mass="43037" checksum="307413329D89218D" modified="2005-10-11" version="1">MATRSRKPPLQFSLRLRRALGGRAGWSGLALQVLFVAILAWIGYEIVANARGNLEAQNTASGLGFMSNTAGFGVSQTLIPYSQTDSYTRVFLVGLVNTLVVAAVGIFFATVIGFMVALGRLSPNWLISRISGGYVELVRNLPLLFQILFWYLAVLRMLPSPKQSISILDGVFLSNRGLAIPRPVGQEGLIAFAVAIAVAIIGCMVLRSHSRRSLFRTGRALVVWPYALGLLIFLPAVAIAVFGIPFTFEMPVLKGFNFSGGARLIPEFVALAIALSTYTAAFIAEVVRAGILSVHKGQMEAGAALGLSRGSTLRLIVVPQAMRVVLPPLTNQYLNLTKNSSLAVAIGYPELFSVFAGTTLSQTGQAIEILAITMGVYLLISLVTSGIMSIYSRRVARSVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSR3</accession>
    <name>Q3SSR3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1417</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314687.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1417"/>
    <dbReference type="EnsemblBacteria" id="ABA04678">
        <property type="protein sequence ID" value="ABA04678"/>
        <property type="gene ID" value="Nwi_1417"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1417"/>
    <dbReference type="eggNOG" id="ENOG50339JI">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_179280_2_0_5"/>
    <dbReference type="OMA" id="MPIVSWM"/>
    <dbReference type="OrthoDB" id="2039320at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021265">
        <property type="entry name" value="DUF2842"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11003">
        <property type="entry name" value="DUF2842"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="68" mass="7617" checksum="597C552915A3E030" modified="2005-10-11" version="1">MRIRTRKFLGTIALLVLVVVWSLLGMTIAQTPWLADSRLYQAIFYVVAGLGWVLPAMPIVSWMAKPDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSR7</accession>
    <name>Q3SSR7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase, group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1413</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314683.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1413"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04674">
        <property type="protein sequence ID" value="ABA04674"/>
        <property type="gene ID" value="Nwi_1413"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1413"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_0_3_5"/>
    <dbReference type="OMA" id="PHAWSFE"/>
    <dbReference type="OrthoDB" id="635734at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13579">
        <property type="entry name" value="Glyco_trans_4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="2">
        <location>
            <begin position="20"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="197"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="377" mass="40577" checksum="BD18C06F8D9DFB48" modified="2005-10-11" version="1">MTGPPDPPLRILHAVRAPVGGIIRHILDVANGQADRGHLVGIVADSLTGGERADTAFAEIAPRMKLGIHRIPIRRNPHPSDLFAWMRFAALVGKLKPQVLHGHGAKAGAFVRLKTSSKDRIRVYTPHGGSLHFPPTTMKGMFYSRLERALMNRTDLFLFESAFARNTYQRIVGNPAGLVKCVFNGVTAGEFDPVPLAADATDVVYVGEFRHIKGADLLIDAMARLREGGRHLTLTLAGDGEETEALKAQVQRLSLGESVRFIGHVKARHGFSKGRLLIVPSRGDSMPYVVIEAAAAGVPMIAANVGGIPEIFDQHTDALFVPGNVTALADAVAAALDDPAAAAARAKSLRERILMHFSQTAMVEGIMAGYRETFARS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSU9</accession>
    <name>RS5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsE</name>
        <name type="ordered locus">Nwi_1381</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">With S4 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Contacts proteins S4 and S8.</text>
    </comment>
    <comment type="domain">
        <text>The N-terminal domain interacts with the head of the 30S subunit; the C-terminal domain interacts with the body and contacts protein S4. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314655.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSU9"/>
    <dbReference type="SMR" id="Q3SSU9"/>
    <dbReference type="STRING" id="323098.Nwi_1381"/>
    <dbReference type="EnsemblBacteria" id="ABA04642">
        <property type="protein sequence ID" value="ABA04642"/>
        <property type="gene ID" value="Nwi_1381"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1381"/>
    <dbReference type="eggNOG" id="COG0098">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065898_2_2_5"/>
    <dbReference type="OMA" id="KRGCGSW"/>
    <dbReference type="OrthoDB" id="1505038at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01307_B">
        <property type="entry name" value="Ribosomal_S5_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_S5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005712">
        <property type="entry name" value="Ribosomal_S5_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005324">
        <property type="entry name" value="Ribosomal_S5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_S5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018192">
        <property type="entry name" value="Ribosomal_S5_N_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718">
        <property type="entry name" value="PTHR13718"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03719">
        <property type="entry name" value="Ribosomal_S5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01021">
        <property type="entry name" value="rpsE_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00585">
        <property type="entry name" value="RIBOSOMAL_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000230351" description="30S ribosomal protein S5">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="S5 DRBM" evidence="1">
        <location>
            <begin position="23"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="191" mass="20743" checksum="CB42B3AC8EBCE92B" modified="2005-10-11" version="1">MAGERERGGRERSRDREERDSEFVDKLVHINRVAKVVKGGKRFGFAALVVIGDQKGRVGFGHGKAREVPEAIRKATESAKRNLTRVPLREGRTLHHDIAGRHGAGRVYLRAAPAGTGIIAGGPMRAVFETLGIQDVVAKSVGSSNPYNMVRATFDALKHQDSPRSVSARRNIKVSTLQSRRVGGDAEAVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="126" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSY5</accession>
    <name>Q3SSY5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Transcription termination/antitermination protein NusG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">nusG</name>
        <name evidence="9" type="ordered locus">Nwi_1345</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Participates in transcription elongation, termination and antitermination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the NusG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314624.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1345"/>
    <dbReference type="EnsemblBacteria" id="ABA04606">
        <property type="protein sequence ID" value="ABA04606"/>
        <property type="gene ID" value="Nwi_1345"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1345"/>
    <dbReference type="eggNOG" id="COG0250">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067287_1_0_5"/>
    <dbReference type="OMA" id="DGPFNGF"/>
    <dbReference type="OrthoDB" id="2027986at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006354">
        <property type="term" value="P:DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032784">
        <property type="term" value="P:regulation of DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00948">
        <property type="entry name" value="NusG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006645">
        <property type="entry name" value="NGN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036735">
        <property type="entry name" value="NGN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043425">
        <property type="entry name" value="NusG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001062">
        <property type="entry name" value="Transcrpt_antiterm_NusG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015869">
        <property type="entry name" value="Transcrpt_antiterm_NusG_bac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30265">
        <property type="entry name" value="PTHR30265"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30265:SF2">
        <property type="entry name" value="PTHR30265:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02357">
        <property type="entry name" value="NusG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00338">
        <property type="entry name" value="NUSGTNSCPFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00738">
        <property type="entry name" value="NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82679">
        <property type="entry name" value="SSF82679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00922">
        <property type="entry name" value="nusG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01014">
        <property type="entry name" value="NUSG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 5 6" id="KW-0889">Transcription antitermination</keyword>
    <keyword evidence="3 5 6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 5 6" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="NGN" evidence="7">
        <location>
            <begin position="2"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="8">
        <location>
            <begin position="122"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA04606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="176" mass="19949" checksum="C72A3E40482FAD3B" modified="2005-10-11" version="1">MSMRWYIVHAYSNFEKKVSESIREQAKQRGLEDLFEQVLVPTEKVTEVRRGRKVDTERKFFPGYVLVKMNLTDEAFHLIKNTPKVTGFLGAENKPMPISEAEAMRIMHQVQEGVERPKASVSFEVGENVRVADGPFASFSGVVEEVDEARSRVKVAVSIFGRATPVELEFGQVEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST37</accession>
    <name>Q3ST37_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Integrase, catalytic region</fullName>
            <ecNumber evidence="3">2.7.7.49</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1293</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04554">
        <property type="protein sequence ID" value="ABA04554"/>
        <property type="gene ID" value="Nwi_1293"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1293"/>
    <dbReference type="eggNOG" id="COG2801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027402_15_1_5"/>
    <dbReference type="OMA" id="PQMADES"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="127"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="329" mass="37887" checksum="5BC35BA851CF0FB8" modified="2005-10-11" version="1">MGQVLHGSATTTEAIRRAIQNSEESLRALSKRYGINQKTVAKWKKRTSVADLPTGPKDPRSTVLSSEEEAIIVAFRKHTLLPLDDCLYALQPTIPHLSRSSLHRCLHRHGISRLPEVEGDKPIRKTFRSYPIGYFHIDIAEVQTAEGKLRLFVAIDRTSKFAYAELHQEAGKMVAAQFLRNLIVAVPYAIHTVLTDNGIQFTNHARHKYAFHHIFDRVCDENGIEHRLTRINHPWTNGQVERMNRTIKDATVKRFHYDNHDQLRRHLQDFIKAYNFGRRLKTLKAPHTLRVHLQTMDFRARSIHHRSNPSNAGTEHLGHRLIKSQPDAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST73</accession>
    <name>Q3ST73_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1257</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1257"/>
    <dbReference type="EnsemblBacteria" id="ABA04518">
        <property type="protein sequence ID" value="ABA04518"/>
        <property type="gene ID" value="Nwi_1257"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1257"/>
    <dbReference type="eggNOG" id="ENOG50335XC">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081277_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="233"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="298" mass="32729" checksum="867443865AD8BEE6" modified="2005-10-11" version="1">MAQPQCGALATRPLPRLRWTRLRARRPAAVRHGPDRSCLAAFALLGRLARWPEGGSDRRPFSLWNLDEDIAMSTRKPATHAPKTVLADDPDDRKGRLKTIGGSQSDHWNNTLANQAVQALWVKNSDDQERDRQLSATVAGLTGIGPKDELEGMMAVQLVAAHNAAMECYRRAMIGEQTFEGRRENLNQANKLSRTWATLLDALNKHRGKGQQKVTVEHVHVHAGGQAVVGTIERPGGGDRTETEEQPHAKQIAGQIAHAPEPTLPCPDAGRDAVPVARDGERTLPDARRQGARRGQRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST93</accession>
    <name>Q3ST93_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">MotA/TolQ/ExbB proton channel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_1236</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MotA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314527.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1236"/>
    <dbReference type="EnsemblBacteria" id="ABA04498">
        <property type="protein sequence ID" value="ABA04498"/>
        <property type="gene ID" value="Nwi_1236"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1236"/>
    <dbReference type="eggNOG" id="COG1291">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079895_1_0_5"/>
    <dbReference type="OMA" id="YAPAWGM"/>
    <dbReference type="OrthoDB" id="897037at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097588">
        <property type="term" value="P:archaeal or bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000540">
        <property type="entry name" value="Flag_MotA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002898">
        <property type="entry name" value="MotA_ExbB_proton_chnl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01618">
        <property type="entry name" value="MotA_ExbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01307">
        <property type="entry name" value="MOTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0283">Flagellar rotation</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="36"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="143"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="MotA_ExbB" evidence="10">
        <location>
            <begin position="101"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA04498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="257" mass="28161" checksum="2DC6A5A48DB2388A" modified="2005-10-11" version="1">MDIMTGAGLAFGMIVIAMMVFMGGDLHMFVSEHAMIIIFGGSIAATMIRFPLGAILHGLPLGAKYAFTMSRLSARDLVDELARIAEISRKQGPVGLEKVETTEPFLAKGVRFVADGYDLEFIRDNLERDRDNFLMHLDEGSKIYRAIGDCAPAFGMIGTLIGMVQMFANMTDPSKLGPFMATALLATLYGALVANLFCLPIADKLHGKLLDEETNRTLIIDGILMIRDSKSPALVREMLLAYLPEKHRHEEGEPVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STC8</accession>
    <name>Q3STC8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cation efflux protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_1201</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314492.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1201"/>
    <dbReference type="EnsemblBacteria" id="ABA04463">
        <property type="protein sequence ID" value="ABA04463"/>
        <property type="gene ID" value="Nwi_1201"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1201"/>
    <dbReference type="eggNOG" id="COG0053">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044569_0_0_5"/>
    <dbReference type="OMA" id="IVNFHCR"/>
    <dbReference type="OrthoDB" id="381612at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1350">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027470">
        <property type="entry name" value="Cation_efflux_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16916">
        <property type="entry name" value="ZT_dimer"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="SSF160240"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="SSF161111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="181"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="ZT_dimer" evidence="8">
        <location>
            <begin position="219"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="ZT_dimer" evidence="8">
        <location>
            <begin position="293"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="ZT_dimer" evidence="8">
        <location>
            <begin position="395"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA04463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="460" mass="48972" checksum="64E460FD6B2FEE06" modified="2005-10-11" version="1">MSRLAARSKTKVAAISIIASASMAAIKLVVGVAIGSLALISEALHSSIDLVATIVTWIVVRISDRPADAEHHYGHGKIESLSALGIIAMLYVLAGGIVVQAYSHLREGAAPPTLSAIPFIVLVTDIIVNAWRARALHRAALDTRSQALEADALHFASDVLGSVAVIIGLTLSGLGYAWGDAAAAIGVAILISLLGLRLGRATVETLLDRAPEGAAERAGAAIRALPDVVDLKRLRVRMVGPRHFVDVIVEVPRTYPIDRIDAIKQQAQDAVSAALGDADLTFTAIPVARDNESVRERIMVIARNLGLAIHHVTVHDLGAKLTVSIDLEVDGEMALVDAHAIAHRLEHDIRHEFGADVEVDTHIEPLEPELPLGTDAAPGRIHAIRTALSGFAAGGAIHDIHNVRVRDTEAGEIVNFHCRAAPSMSVARVHESVDEIERALRRAFPSVKRVISHAEPPRES</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STJ8</accession>
    <name>Q3STJ8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_1131</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314424.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1131"/>
    <dbReference type="EnsemblBacteria" id="ABA04393">
        <property type="protein sequence ID" value="ABA04393"/>
        <property type="gene ID" value="Nwi_1131"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1131"/>
    <dbReference type="eggNOG" id="COG3115">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060737_0_0_5"/>
    <dbReference type="OMA" id="FGEMSVN"/>
    <dbReference type="OrthoDB" id="1662206at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044781">
        <property type="term" value="P:bacterial-type flagellum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022781">
        <property type="entry name" value="Flagellar_biosynth_FliO"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04347">
        <property type="entry name" value="FliO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="89"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="185"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="230"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="285" mass="30618" checksum="307E5CDE9EE060FC" modified="2005-10-11" version="1">MQAVTFVIAFIVVLALIAGAAWLVRRFAGDRLGVNANRGRMPRLAVIDAAAVDGRRRLVLVRRDNVEHLLMIGGPTDIVVEHNIVRAAPGRDQAPQRPLPGSETPRLPPLAETGWTESDSTRSIPPDHPEPTEPELPRPAPRPAFADTVRKPVAAERRSDPLADFTPEPMSRTDIRGTRPARQEPLVPRPAPAPAPLPPASDADQNLAEMAERLEAALRRPTGGKSPPATPVTSEPPPRPRGSEFSPPPASRSEPPPVAPPGPKSGFEHLENEMASLLGRTKSPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STK1</accession>
    <name>Q3STK1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1128</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314421.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04390">
        <property type="protein sequence ID" value="ABA04390"/>
        <property type="gene ID" value="Nwi_1128"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1128"/>
    <dbReference type="HOGENOM" id="CLU_2586156_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="80" mass="9314" checksum="F98DBE30FA7CFC4E" modified="2005-10-11" version="1">MIIDAQMRFHRNLYRLAISIRIPIEGKLNVDRHRSVEGSKSTLFPANRKQSSGLFFRRITLRELAAIQAHPKDKFHSITL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-03-03" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STP0</accession>
    <name>FENR_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ferredoxin--NADP reductase</fullName>
            <shortName evidence="1">FNR</shortName>
            <shortName evidence="1">Fd-NADP(+) reductase</shortName>
            <ecNumber evidence="1">1.18.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">Nwi_1089</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + NADP(+) + 2 reduced [2Fe-2S]-[ferredoxin] = NADPH + 2 oxidized [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:20125"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.18.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="1">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ferredoxin--NADP reductase type 2 family.</text>
    </comment>
    <dbReference type="EC" id="1.18.1.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314385.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3STP0"/>
    <dbReference type="SMR" id="Q3STP0"/>
    <dbReference type="STRING" id="323098.Nwi_1089"/>
    <dbReference type="EnsemblBacteria" id="ABA04351">
        <property type="protein sequence ID" value="ABA04351"/>
        <property type="gene ID" value="Nwi_1089"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1089"/>
    <dbReference type="eggNOG" id="COG0492">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031864_5_5_5"/>
    <dbReference type="OMA" id="PEKVIYD"/>
    <dbReference type="OrthoDB" id="692968at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004324">
        <property type="term" value="F:ferredoxin-NADP+ reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01685">
        <property type="entry name" value="FENR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022890">
        <property type="entry name" value="Fd--NADP_Rdtase_type_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000364886" description="Ferredoxin--NADP reductase">
        <location>
            <begin position="1"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01685"/>
        </source>
    </evidence>
    <sequence length="342" mass="37241" checksum="CB9E75427604918C" modified="2005-10-11" version="1">MSEAIKTDVLIIGAGPCGLFAVFELGLLDMKVHLVDILDKIGGQCAELYPEKPIYDIPGIPLVTGQGLTEALLEQIKPFNPTFHLNEMVESIEKIGDPGFRVTTDAGQVFECKVVVVSAGGGSFQPKRPPVPGIEAYENISVFYAVRKMEQFRDKEILIVGGGDSALDWTLNLHPLAKRITLLHRRDEFRAAPHSVEQMRKLVADGTMDLKIGQVTALEGADGKLTGAQVKGSDNTTSTVSCDTMLPFFGLTMKLGPVANWGLHLENNLIPVETASFETNVPGIFAIGDINTYPGKLKLILSGFHEGALMAQKAHRYVYPDKRLVFQYTTSSSSLQKKLGVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STQ4</accession>
    <name>Q3STQ4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mannose-1-phosphate guanylyltransferase</fullName>
            <ecNumber evidence="3">2.7.7.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_1075</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-mannose 1-phosphate + GTP + H(+) = diphosphate + GDP-alpha-D-mannose</text>
            <dbReference type="Rhea" id="RHEA:15229"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
            <dbReference type="EC" id="2.7.7.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the mannose-6-phosphate isomerase type 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.13" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314371.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1075"/>
    <dbReference type="EnsemblBacteria" id="ABA04337">
        <property type="protein sequence ID" value="ABA04337"/>
        <property type="gene ID" value="Nwi_1075"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1075"/>
    <dbReference type="eggNOG" id="COG0662">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0836">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035527_1_0_5"/>
    <dbReference type="OMA" id="LIVCNEK"/>
    <dbReference type="OrthoDB" id="224217at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004475">
        <property type="term" value="F:mannose-1-phosphate guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006375">
        <property type="entry name" value="Man1P_GuaTrfase/Man6P_Isoase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001538">
        <property type="entry name" value="Man6P_isomerase-2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01050">
        <property type="entry name" value="MannoseP_isomer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01479">
        <property type="entry name" value="GMP_PMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transferase" evidence="6">
        <location>
            <begin position="7"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="MannoseP_isomer" evidence="7">
        <location>
            <begin position="316"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA04337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="470" mass="51586" checksum="5F7D9B927D9A41C6" modified="2005-10-11" version="1">MSERIVPLIMCGGAGTRLWPASREGRPKQFLPLFGPRSTFQDTLRRVSDPVLFERPIVVTNAAYRFMVLEQLAEIGLEADILLEPARRDSGPAIAAGAAFAQMRDSEAVVLALAADHVVRDTQAFVSACRQGLAAANGGRIVTFGVRPQRPATEYGYINPGPAIAGEVREVTTFVEKPDPQTAAGYLDAGYLWNSGNFMFRAAVLLDEYRNVDPDSVSAATDAVSRAGRDLGFVILDPEAFSRARAISIDYAVMEKTTRAAVVPVDCGWSDVGSWHAVWELSDKDGQGNAAQGIAVFEDSRNCNVATDKALVALEGVDDLVVVATQDAVLVSRQKDANGLKRLVTKLKSLAPQVTDEHLRVHRPWGAYQSIDRGERHQVKRITVKAGDRLSLQKHHHRSEHWIVVRGTARVTVDDLVKIVHENESIYIPIGAVHRLENPGKIPLELIEVQTGSYLGDDDIVRIEDDYRRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STR4</accession>
    <name>Q3STR4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 8">UDP-glucose 4-epimerase</fullName>
            <ecNumber evidence="5 8">5.1.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_1065</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>UDP-alpha-D-glucose = UDP-alpha-D-galactose</text>
            <dbReference type="Rhea" id="RHEA:22168"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="5.1.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 8">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 8">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.2" evidence="5 8"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314361.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1065"/>
    <dbReference type="EnsemblBacteria" id="ABA04327">
        <property type="protein sequence ID" value="ABA04327"/>
        <property type="gene ID" value="Nwi_1065"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1065"/>
    <dbReference type="eggNOG" id="COG1087">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_10_5"/>
    <dbReference type="OMA" id="GEHLICN"/>
    <dbReference type="OrthoDB" id="1597849at2"/>
    <dbReference type="UniPathway" id="UPA00214"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003978">
        <property type="term" value="F:UDP-glucose 4-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05247">
        <property type="entry name" value="UDP_G4E_1_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005886">
        <property type="entry name" value="UDP_G4E"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01179">
        <property type="entry name" value="galE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7 8" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="9">
        <location>
            <begin position="3"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA04327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="336" mass="35995" checksum="13E6961199030046" modified="2005-10-11" version="1">MTVLVTGGAGYIGSHMVLALAEAGENVVVIDNLSTGFSSALPEGVPLFIGDAGDENLVENVIAQHGIESIIHFAGSVVVPDSMRDPLAYYRNNTMTTRNLLNAAVKCGVNRFIFSSTAAVYGNPDQMPVAEEAPTRPLSPYGSSKLMTEIMLHDVGSAHDLNYVVLRYFNVAGADPQARIGLQTAGATHLLKIAVEVATGQRAKIDVFGTDYPTPDGSCIRDFIHVSDLAQAHRAALTYLRNGGGSTTLNCGYGRGYSVLETIEAVRRVCGRNFAVHYSDRRPGDIMTMIADTSRIRAALDWTPQYDDLATIAAHALAWEEKLAQRHATDALAVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STV6</accession>
    <name>Q3STV6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">IMP dehydrogenase</fullName>
            <ecNumber evidence="4">1.1.1.205</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1023</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.205" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314319.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1023"/>
    <dbReference type="EnsemblBacteria" id="ABA04285">
        <property type="protein sequence ID" value="ABA04285"/>
        <property type="gene ID" value="Nwi_1023"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1023"/>
    <dbReference type="eggNOG" id="COG0517">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040681_1_0_5"/>
    <dbReference type="OMA" id="HGVADDW"/>
    <dbReference type="OrthoDB" id="2014094at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003938">
        <property type="term" value="F:IMP dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007055">
        <property type="entry name" value="BON_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017080">
        <property type="entry name" value="UCP036990_CBS_BON"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04972">
        <property type="entry name" value="BON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036990">
        <property type="entry name" value="UCP036990_CBS_BON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50914">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="7"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="94"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="BON" evidence="2">
        <location>
            <begin position="157"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="243" mass="27137" checksum="17F2764E88769276" modified="2005-10-11" version="1">MRTHQIMTHKLITVKADTPIVEAAKLMLESHISGLPVVDDAGRLLGIVSESDFMRRSEIGTHGPRIRWLDFLMGTEKAAIDFVREHGRKVSAIMTRETLFTATEDMPLEELVRLMERQNIKRLPVIRGDLLVGIVTRADLLRAVASLARDVPDPTADDDHVRRRVIASIEKNEWQPTGLGVTVRDGIVYLSGMITDEAHRQAAIVAAENVSGVKLVRDHLYLFDPVSGLTFRSPKDECRTKTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STX0</accession>
    <name>Q3STX0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein involved in outer membrane biogenesis</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1009</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1009"/>
    <dbReference type="EnsemblBacteria" id="ABA04271">
        <property type="protein sequence ID" value="ABA04271"/>
        <property type="gene ID" value="Nwi_1009"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1009"/>
    <dbReference type="eggNOG" id="COG2982">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012870_2_0_5"/>
    <dbReference type="OMA" id="FPWLGLE"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007844">
        <property type="entry name" value="AsmA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05170">
        <property type="entry name" value="AsmA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="17"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="AsmA" evidence="3">
        <location>
            <begin position="15"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="AsmA" evidence="3">
        <location>
            <begin position="353"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="631"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="656" mass="69818" checksum="58C87CB49582C552" modified="2005-10-11" version="1">MLIDDDPRTMAQGMKRLGISVAAILGVAVIGLTGLISMSRLLDHDALRDAVETQIRTVTGLDFVADGRIDVSVFPGSYVSFHDVRIKGGDADAPALSVEALTANLRLFPLLMQRFQIADVMLLHPHIRVRRAADGRSNWTPFMETIARNMKPGADNPLSFSEIRIQNGELSYEDGDGRISENLHGIDLSLAWPSISRSFAATGQFDWRGERVDGSISLGDFLAALSGERSGLKIKVASPRLKFAFDGTVANPVSLIMEGTLSADSTSLREALRWIGQPPPGSGGFDRFSLKARASVIGSSVALTNVNIEIDGNVAEGVMTYANNGRQTLQATLAAGTLDFTPYVKTIRLLASGARDWNRQLFDLRALSATDLDMRLSAAKVIVGSTRLGKTALGANLRNGSLALSIGEAQIYDGVAKGSFAISHAEEAADVKAQFQLIDVDLQACVSDLFGTGRLSGRGNLNVSLEARGSSPYGLAQSLDGTAVLNGHDGAISGFNVEQLLKRLERRPLSGGGSFRSGKTPFDKLNVTLNFDDGVAVATDVRVEGPAARLTLTGTASVPSRDYDLKGTASLTSAAGADNFELPFVVQGPWDDPLVFPDPESLIRRSKASAPLLDAVRDRRARDAVRSVIERLTDHKPVPEAAVPDAASGSGTSHSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STX4</accession>
    <name>Q3STX4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FAD dependent oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314305.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1005"/>
    <dbReference type="EnsemblBacteria" id="ABA04267">
        <property type="protein sequence ID" value="ABA04267"/>
        <property type="gene ID" value="Nwi_1005"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1005"/>
    <dbReference type="eggNOG" id="COG0579">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024775_1_1_5"/>
    <dbReference type="OMA" id="WGVSKAN"/>
    <dbReference type="OrthoDB" id="1371190at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAO" evidence="1">
        <location>
            <begin position="7"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="368" mass="39017" checksum="2CCD376957B0CB79" modified="2005-10-11" version="1">MDRIECVVVGAGVIGLAVARRLARAGREVIVLEAAENIGTGTSSRNSEVIHAGIYYPSGSLMARTCVSGRPALYAYCREHSIPHSMCGKLIVAATEGEVDRLQIIRAQAEANGVEDIRFLNGDEARALEPALRCVAGLLSPSTGIIDSHAFMLALRGDAERDGAAFAFHTPLSGARATPHGLEIKTGGDAPMTLVCDLLVNAAGLDAPAVARRIDGMPVERIPPAYLAKGSYFSCSVRAPFSRLIYPVPEPGGLGVHLTLDLAGQPRFGPDVEWIETPNYDVDPARATRFYPAIRRYWPDLPDDTLIPAYSGIRPKIVPPAVAAQDFMIQGPADHGLSGLINLFGIESPGLTASLAIADHVAALAAQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU09</accession>
    <name>Q3SU09_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0970</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314273.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04232">
        <property type="protein sequence ID" value="ABA04232"/>
        <property type="gene ID" value="Nwi_0970"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0970"/>
    <dbReference type="HOGENOM" id="CLU_2650797_0_0_5"/>
    <dbReference type="OMA" id="APFFHYE"/>
    <dbReference type="OrthoDB" id="2168178at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="76" mass="8848" checksum="0CBC4082792F838D" modified="2005-10-11" version="1">MPIKLDETKFALDDHAPFGKLWGRLLIEVVDKETDIRQTVHLEVLLDKGDRTNFLQYEKLARDEAKKLLTQVAEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU30</accession>
    <name>Q3SU30_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aspartate/glutamate/uridylate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0949</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314252.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0949"/>
    <dbReference type="EnsemblBacteria" id="ABA04211">
        <property type="protein sequence ID" value="ABA04211"/>
        <property type="gene ID" value="Nwi_0949"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0949"/>
    <dbReference type="eggNOG" id="COG0528">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1008069_0_0_5"/>
    <dbReference type="OMA" id="RTDAGCF"/>
    <dbReference type="OrthoDB" id="1043239at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04255">
        <property type="entry name" value="AAK_UMPK-MosAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030669">
        <property type="entry name" value="MoSto_subunit_alpha/beta"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039097">
        <property type="entry name" value="MoSto_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AA_kinase" evidence="1">
        <location>
            <begin position="38"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="269" mass="28340" checksum="85968940096953EE" modified="2005-10-11" version="1">MATTSELEQLLMQRSLTDAQLLAAADASANFRILPDATVLKIGGQSVIDRGRAAVYPLVDEIVAARKDHKLLIGTGAGTRARHLYSISAGLGLPAGVLSQLGASVASQNAIMLGQLLAKHGISAVDGAGLSAVPLYLSEVNVVIFSGMPPYGLWMRPDSEGVIPPYRTDAGCFLIAEQFGCKAMIFVKDEDGLYTANPKTSKGATLIPKISVAEMKAKGLQDSILEFRMLDLLESARNVREVQVVNGLVPGNLTRALSGEHVGTIITAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU79</accession>
    <name>Q3SU79_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NAD-dependent epimerase/dehydratase</fullName>
            <ecNumber evidence="3">5.1.3.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0898</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.2" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0898"/>
    <dbReference type="EnsemblBacteria" id="ABA04162">
        <property type="protein sequence ID" value="ABA04162"/>
        <property type="gene ID" value="Nwi_0898"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0898"/>
    <dbReference type="eggNOG" id="COG0451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_6_0_5"/>
    <dbReference type="OMA" id="SHRVPWK"/>
    <dbReference type="OrthoDB" id="1928091at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003978">
        <property type="term" value="F:UDP-glucose 4-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="2">
        <location>
            <begin position="16"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="342" mass="37289" checksum="9D2915DF8C2C28FF" modified="2005-10-11" version="1">MLQPRARMALAGRRPILVTGGNGFIGQHLVAALRRRHGVVRVLDLQPPPPGPVPEFIQGTILDPHDVRRALDGVDTVYHLAAISHLWTANPADFERVNQHGTELMLAAARERGVRNIVHCSTEAILFPYRRGGTTMPQRAEDMPGPYTRSKFMAEQIAREAAADGLRVVIANPTVPIGPGDHNFTEPTRMLDLFARKSPPMVLDSMLNLVDVRDVAAGLILAGERGRAGERYILGGENVSVRELVRRVGSLCGRSANVHALPASLALAIGAASEWFEGQVIQRTPRVSIEAVRIALRSIPLDTRKAETELGYLPRPIDDALTDAIAWLTRRDADAAQPRVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU83</accession>
    <name>Q3SU83_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Sigma 24, ECF subfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0894</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314199.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0894"/>
    <dbReference type="EnsemblBacteria" id="ABA04158">
        <property type="protein sequence ID" value="ABA04158"/>
        <property type="gene ID" value="Nwi_0894"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0894"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_12_3_5"/>
    <dbReference type="OMA" id="GQIANGR"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="2">
        <location>
            <begin position="9"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="3">
        <location>
            <begin position="108"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="165" mass="19087" checksum="3B5DBD07069894EE" modified="2005-10-11" version="1">MTWDLQKLFIRHAREIDRFLRRRGHNAETAADITQDTFLRVLASPPGEARTNHNPKAYLYQVSRSLSINHLRRESLIQTTDLDEAGAIADPTPSAEKIIYSRQCLAQTRKALAELPERTRMAFEMHRLCDKTIQEVADELGVSTSRAWKLIRNGYRHLLTRVDDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU89</accession>
    <name>Q3SU89_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Outer membrane ferric siderophore receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0888</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04152">
        <property type="protein sequence ID" value="ABA04152"/>
        <property type="gene ID" value="Nwi_0888"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0888"/>
    <dbReference type="HOGENOM" id="CLU_2918016_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036942">
        <property type="entry name" value="TonB_rcpt_b-brl_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0675">Receptor</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="61" mass="6781" checksum="7F45337D4A0A13B8" modified="2005-10-11" version="1">MMTLARAPNDESLRDTFQGREHFKQPGAITPYYGAILDLADVLSAYASYTGIFRPQAAQDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUD0</accession>
    <name>Q3SUD0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0847</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the N(4)/N(6)-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314158.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0847"/>
    <dbReference type="REBASE" id="11317">
        <property type="entry name" value="M.NwiORF847P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04111">
        <property type="protein sequence ID" value="ABA04111"/>
        <property type="gene ID" value="Nwi_0847"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0847"/>
    <dbReference type="eggNOG" id="COG0863">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024927_3_0_5"/>
    <dbReference type="OMA" id="IDAGHYV"/>
    <dbReference type="OrthoDB" id="1487464at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006306">
        <property type="term" value="P:DNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002941">
        <property type="entry name" value="DNA_methylase_N4/N6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001091">
        <property type="entry name" value="RM_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01555">
        <property type="entry name" value="N6_N4_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00508">
        <property type="entry name" value="S21N4MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N6_N4_Mtase" evidence="6">
        <location>
            <begin position="24"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="252" mass="27585" checksum="6B8A3A2B49EEBF38" modified="2005-10-11" version="1">MTVTDFSVITGDCRVLMPAQGPFDLILADPPYGDTSLAWDRRVDGWLQLACAALKPSGSLWVFGSLRSFMATADQFADADLRYAQEIVWEKQNGTSFHADRFKRVHELVAQFYRADVPWSAVYNDVQTTPDATARTVRRKMPPPHTGRIDAGHYVSEDGGPRLMRSVIYARNCHGTAIHPTEKPSGLIEILIRTSCPQGGLVGDWFVGSGAAGEACRLAGRRYVGCEIDAGMAELARGRITSVLPFIEGAQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUE1</accession>
    <name>Q3SUE1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GCN5-related N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0836</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314148.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0836"/>
    <dbReference type="EnsemblBacteria" id="ABA04100">
        <property type="protein sequence ID" value="ABA04100"/>
        <property type="gene ID" value="Nwi_0836"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0836"/>
    <dbReference type="eggNOG" id="COG0454">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101288_0_0_5"/>
    <dbReference type="OMA" id="DIAGIRC"/>
    <dbReference type="OrthoDB" id="2055010at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13508">
        <property type="entry name" value="Acetyltransf_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="55"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="171" mass="19655" checksum="E47594A5E148D68B" modified="2005-10-11" version="1">MARLTPPRQLEETDDCAGFDCGRESMNQWFRRHAWRNQQIDVSRSTVIRDAETGMIAGYVTLSVGHIEREYLPKSSQRNRPENIPIFLLGQLAVDTRFQGMGVGRSLMFYALKTAVGMAKEIGCYGVLTHPLDDDVRRFYQNFGFEQLPHDPRRSMIVRIKDLVRNGFDAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUF0</accession>
    <name>Q3SUF0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, XRE family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0826</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314139.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0826"/>
    <dbReference type="EnsemblBacteria" id="ABA04091">
        <property type="protein sequence ID" value="ABA04091"/>
        <property type="gene ID" value="Nwi_0826"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0826"/>
    <dbReference type="eggNOG" id="COG1476">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2406816_0_0_5"/>
    <dbReference type="OMA" id="RECEKPP"/>
    <dbReference type="OrthoDB" id="1933795at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="7"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="96" mass="11033" checksum="F655F49A1A8710FB" modified="2005-10-11" version="1">MAFGQTVRFHRNQKGFGLNEFAERLGVSPAYWSRVEREQENPPRDDLVERVAAILEVKLDDLFCEAGRLPPDLRGDIKAVVTLYRRSRDARNSTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUG9</accession>
    <name>Q3SUG9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Peptidase U35, phage prohead HK97</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0807</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314120.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0807"/>
    <dbReference type="EnsemblBacteria" id="ABA04072">
        <property type="protein sequence ID" value="ABA04072"/>
        <property type="gene ID" value="Nwi_0807"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0807"/>
    <dbReference type="eggNOG" id="COG3087">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024622_2_0_5"/>
    <dbReference type="OMA" id="NSWFRMQ"/>
    <dbReference type="OrthoDB" id="436392at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="689" mass="75110" checksum="79B4CDEDB71543DB" modified="2005-10-11" version="1">MKPAQPPSRGVAPLANALPMQTRLAPVASIEAETRTVEVVWTTGASVRRRRWTGFDTAIDYEEILVVSRDAVDLSRLDAGAPVLDSHSQWTTRAIIGVVERAWIDKGEGRAALRFPKPGVDEAADRLFALVTDGIVRNISVGYHIDKVRVERPKRVGEPERWFVERWTPHELSFVAVGADPGAQVRSAEDAPTFPFELIEPFSRTMETAAMDEPVQTREAAPEASPPNAHTDRAPPAPAANERATPAPPNPSIAAADLDQVRTEERERVGAIFGLADRFKLDRAFADDLVTRGVAIEEARRLILDKLARRDERGAGHTAVSFPAGGLDATVTRREALVEAILHRLAPVAFTMTDRARDYRGMSLVDVARDSLAVAGQATRGMTGNEIVHAATRAAGLHSSSDFPLILAAVAGKRLRQAYQVTPRTFTRWTRGTTAPDFKPMYPTQIGNFPPLKPVMEGAEFTYGTIAEGRETYKLATYGRIVALTRQAIVNDDLRAFDRALNTAGQKAAELESALVYNELLANGVLADGVALFHATHGNLGTAAAISEAALSQAWELMAQQKDISGADAEYISVIPRFILVPPGTRSIEARKMIAAVTPAKAADVNAFANSLEPIEEPRLYKPGGPQPWWLCADPNAIDTIEYAHLEGQTEPFMDQRVGFEVDGVEFKIRHDFAAKALDFRGLFYNPGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUI8</accession>
    <name>Q3SUI8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0788</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314101.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0788"/>
    <dbReference type="EnsemblBacteria" id="ABA04053">
        <property type="protein sequence ID" value="ABA04053"/>
        <property type="gene ID" value="Nwi_0788"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0788"/>
    <dbReference type="eggNOG" id="ENOG502ZQJ6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2899619_0_0_5"/>
    <dbReference type="OMA" id="YRTYIIA"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="62" mass="6379" checksum="7E668447745A860A" modified="2005-10-11" version="1">MNAVLQFGAGYRTYIIAAVLVLVVVVEKGLGIDVPGVDVGSDWLSHVLAALGLGTLRAGITK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUK2</accession>
    <name>Q3SUK2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nitrate reductase (quinone)</fullName>
            <ecNumber evidence="7">1.7.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Nwi_0774</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a quinol + nitrate = a quinone + H2O + nitrite</text>
            <dbReference type="Rhea" id="RHEA:56144"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16301"/>
            <dbReference type="ChEBI" id="CHEBI:17632"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.7.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.7.5.1" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314088.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0774"/>
    <dbReference type="EnsemblBacteria" id="ABA04039">
        <property type="protein sequence ID" value="ABA04039"/>
        <property type="gene ID" value="Nwi_0774"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0774"/>
    <dbReference type="eggNOG" id="COG5013">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_14_1_5"/>
    <dbReference type="OMA" id="PFIHPFN"/>
    <dbReference type="OrthoDB" id="88184at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009325">
        <property type="term" value="C:nitrate reductase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008940">
        <property type="term" value="F:nitrate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042126">
        <property type="term" value="P:nitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02776">
        <property type="entry name" value="MopB_CT_Nitrate-R-NarG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037943">
        <property type="entry name" value="MopB_CT_Nitrate-R-NarG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006655">
        <property type="entry name" value="Mopterin_OxRdtase_prok_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027467">
        <property type="entry name" value="MopterinOxRdtase_cofactor_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006468">
        <property type="entry name" value="NarG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00926">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01580">
        <property type="entry name" value="narG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00551">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00932">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 12" id="KW-0408">Iron</keyword>
    <keyword evidence="11 12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="13">
        <location>
            <begin position="26"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00010312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABA04039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1214" mass="138729" checksum="9EB47D5143A00A8F" modified="2005-10-11" version="1">MSWILDLVNPRERKWEEFYRNRWSHDNVFRSTHGVNCTGGCSWAIYVKDGIITWEMQQTDYPLLERSLPPYEPRGCQRGISASWYVYSPIRVKYPYVRGPLYDLWKEAKASHPDPVQAWASLVGDEQKRSRIQKARGKGGFRRAKWEELVELIAAAALYTARKYGPDRVMGFSPIPAMSMLSYAAGSRFLQLFGGVNMSFYDWYADLPTSFPEIWGDQTDVCESADWYNSKFIVSMAANMNMTRTPDVHFISEARTEGTKFVVLSPDFSQIAKYCDEWIPIQAGQDTALWMAANHVILKEYYIDRQVPYFIDYVKRYTDLPFLVELEPNGNTYKTGRLLRSNRVARYKDVENGEWKMLVLDTATGEPRAFKGQVGDRWGSTHGKWNLSAEDTLDNSPIDPVLSFIDQSDGVVQVGFDDFVNGSVVSRGVPVKRIATDKGEVLVTTGFDIMMSQFGHSRGLEGSFATSYDDEDAPYTPAWQERHTGIGRETAIRFAREFATNAELTNGKSMVIVGASANHWYYNNLCYRSATVALILCGCCGVNGGGINHYVGQEKLAPVAPWNTIAMALDWTKPPRVVQSSTWHYAHSCQWRYEQEFTEYGLTAPNPRWAKGHAIDLEAKSVRCGWMPFTPHFNRNPIELAAEAERAGAKSTADIVTHVVDQVASKKVNFAIDDPDAEESWPRMWFIWRGNAIQSSAKGHEFFLRHYLGAHDNSIAEDRAKGKTQRVKYRDTAPRGKYDLVVDLNFRMNTTSLYSDIVLPTAFWYEKNDLNTTDLHSFLHVLGQAVPPVWESKTDWEIFKLISKKVSELSPLAFSKPVRDVVVQPLMHDTPDELAQPEILDWAEGECKPVPGKSFPHVRVVERDYANLYNKFISFGPKAREDGVSAVGVNIPIKKQYDQMLDNPIMPMPDARHMRCVEWGGKRYPSLEDVLDACNTLLMCAPEANGEVCYQGFHNEEHHVGLPLVDIAEPTRGVSSTFYDLTRQPRRILTSPCWTGLTNDGRAYSAWCMNVERLVPWRTLTGRQSLYLDHQWYLDFGEHIPTYKPRLNPRKTGDIVKSRVDDRSLVLNYITPHGKWNIHSTYKDNHRMLMLSRGMDPVWINDRDAEKVGIEDNDWVEVYNDNGVVVTRANVSRRIQPGTCMYYHAVERTVYIPKSQERKWRGGGHNSLTRTRINPLFLAGGYAQFTYGFNYWGPTGIFTRDTHVVVRKMEKLEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUM8</accession>
    <name>Q3SUM8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Glutamyl-tRNA synthetase, class Ic</fullName>
            <ecNumber evidence="8">6.1.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_0748</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="8"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314067.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0748"/>
    <dbReference type="EnsemblBacteria" id="ABA04013">
        <property type="protein sequence ID" value="ABA04013"/>
        <property type="gene ID" value="Nwi_0748"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0748"/>
    <dbReference type="eggNOG" id="COG0008">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015768_0_3_5"/>
    <dbReference type="OMA" id="WLLRMED"/>
    <dbReference type="OrthoDB" id="1409413at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110165">
        <property type="term" value="C:cellular anatomical entity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006418">
        <property type="term" value="P:tRNA aminoacylation for protein translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 6 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA-synt_1c" evidence="7">
        <location>
            <begin position="7"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA04013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="290" mass="32182" checksum="43F24D478E1BE151" modified="2005-10-11" version="1">MPPPVFRFAPSPNGFLHLGHAFSALMNFESARRGQGRFLLRIEDIDAARCRPEFEAAIYQDLAWLGLSWETPVRRQSEHLADYRAALDRLAALGLLYPSFESRAEIAALVNAREMDGGWPRDPDGAPLYPGTAKSLAATERERRLRSGAPFALRLDMEAARRLAGDLTWIERGSGPDGETGEILARPEAWGDVILARKDIPTSYHLSVVIDDALQGVTEVTRGQDLFRSTAVHRLLQEILDLPQPVYRHHDLIRDAEGKKLSKSSRAIGLRELRSGGMTTAELRSLVGLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUQ0</accession>
    <name>Q3SUQ0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="12">TonB-dependent receptor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_0725</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the TonB-dependent receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0725"/>
    <dbReference type="EnsemblBacteria" id="ABA03991">
        <property type="protein sequence ID" value="ABA03991"/>
        <property type="gene ID" value="Nwi_0725"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0725"/>
    <dbReference type="eggNOG" id="COG4773">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008287_9_0_5"/>
    <dbReference type="OMA" id="AMERIEM"/>
    <dbReference type="OrthoDB" id="169533at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015344">
        <property type="term" value="F:siderophore uptake transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012910">
        <property type="entry name" value="Plug_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037066">
        <property type="entry name" value="Plug_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011662">
        <property type="entry name" value="Secretin/TonB_short_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039423">
        <property type="entry name" value="TonB-dep_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000531">
        <property type="entry name" value="TonB-dep_rcpt_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036942">
        <property type="entry name" value="TonB_rcpt_b-brl_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010105">
        <property type="entry name" value="TonB_sidphr_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32552">
        <property type="entry name" value="PTHR32552"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07715">
        <property type="entry name" value="Plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00593">
        <property type="entry name" value="TonB_dep_Rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00965">
        <property type="entry name" value="STN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01783">
        <property type="entry name" value="TonB-siderophor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <keyword evidence="4 8" id="KW-0798">TonB box</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229327" evidence="10">
        <location>
            <begin position="36"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="domain" description="STN" evidence="11">
        <location>
            <begin position="74"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="613"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA03991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="633" mass="68374" checksum="EE8543CD80B23E71" modified="2005-10-11" version="1">MAGRSVSGVAAIRMARLLASISFAALLLPSQAAFAQTAPSSAPSAGALQQSITFAIPPQSLSSGIVAFSRAAGIDLVFDGAVPRGARTSGVSGTFTVRDGVNRLLAGTGLSARFTNARTVQIVNPSAAGGVSGAAPSGAISLDTIDVQGETAWGPANGFVASRSATATKTDTPIIEVPQSISVVTRDQIEIRRNQTLNETLQYTPGVFAESSGMQHSGPTFQIRGFSAVPADGPIFLNGLRTINNGDIEPFGLERIEVMRGPASVLYGQNQPSGVIALVTKRPTEGPVHEVQLRGGSFGQKSGAFDFGGPVTEDRTLLYRFTGLLREGGNGIDFSNDQRAFVSGAVTWRPTDATELTAFSLFQKDKGRWNYGLPAQGTALPNPNGRIPVTRFIGEPDLDYNVTERAVVGYNLQHRATESLTFRQNLQYARENWDYRNATPIGLQADLRTLDRSYETLRASWDTFAIDNQAELKTTTGVLHHTVLFGVDYRWRRYNSLGLWGGVASPIDVFAPVYGQPVLISADNYGSRQTQNFPGIYLQDQIKLDRWILTFGGRHDWAESKTVNAFSAAVTNQNDRAFTRRAGLGYEFDSGVVPYVSYSESFMPVSGTTFDQVPFRPKPERSTRQASNTSRMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUT7</accession>
    <name>Q3SUT7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">3-demethylubiquinone-9 3-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0688</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314010.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0688"/>
    <dbReference type="EnsemblBacteria" id="ABA03954">
        <property type="protein sequence ID" value="ABA03954"/>
        <property type="gene ID" value="Nwi_0688"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0688"/>
    <dbReference type="eggNOG" id="COG3865">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046006_22_0_5"/>
    <dbReference type="OMA" id="DQDEVDY"/>
    <dbReference type="OrthoDB" id="1889762at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06588">
        <property type="entry name" value="PhnB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009725">
        <property type="entry name" value="3_dmu_93_MTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028973">
        <property type="entry name" value="PhnB-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06983">
        <property type="entry name" value="3-dmu-9_3-mt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF021700">
        <property type="entry name" value="3_dmu_93_MTrfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="3-dmu-9_3-mt" evidence="1">
        <location>
            <begin position="5"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="159" mass="17172" checksum="158F0A226EC46308" modified="2005-10-11" version="1">MATAKNTICLWYDRDAEAAARFYAATFPDSSVSAVHHAPTDYPSGKAGDVLTVEFTVAGIPCLGLNGGPAFKHNEAFSFQIATDDQEETDRYWNAIVGNGGQESACGWCKDKWGISWQITPRVLTEAMAAGGDEAKRAFSAMMTMKKIDVAAIEKARRG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUZ1</accession>
    <name>DXS_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">1-deoxy-D-xylulose-5-phosphate synthase</fullName>
            <ecNumber evidence="1">2.2.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">1-deoxyxylulose-5-phosphate synthase</fullName>
            <shortName evidence="1">DXP synthase</shortName>
            <shortName evidence="1">DXPS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dxs</name>
        <name type="ordered locus">Nwi_0633</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glyceraldehyde 3-phosphate + H(+) + pyruvate = 1-deoxy-D-xylulose 5-phosphate + CO2</text>
            <dbReference type="Rhea" id="RHEA:12605"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="2.2.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
        <text evidence="1">Binds 1 thiamine pyrophosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the transketolase family. DXPS subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.7" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SUZ1"/>
    <dbReference type="SMR" id="Q3SUZ1"/>
    <dbReference type="STRING" id="323098.Nwi_0633"/>
    <dbReference type="EnsemblBacteria" id="ABA03900">
        <property type="protein sequence ID" value="ABA03900"/>
        <property type="gene ID" value="Nwi_0633"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0633"/>
    <dbReference type="eggNOG" id="COG1154">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009227_1_4_5"/>
    <dbReference type="OMA" id="QVGYHAQ"/>
    <dbReference type="UniPathway" id="UPA00064">
        <property type="reaction ID" value="UER00091"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008661">
        <property type="term" value="F:1-deoxy-D-xylulose-5-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052865">
        <property type="term" value="P:1-deoxy-D-xylulose 5-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02007">
        <property type="entry name" value="TPP_DXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00315">
        <property type="entry name" value="DXP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005477">
        <property type="entry name" value="Dxylulose-5-P_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020826">
        <property type="entry name" value="Transketolase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005474">
        <property type="entry name" value="Transketolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43322">
        <property type="entry name" value="PTHR43322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13292">
        <property type="entry name" value="DXP_synthase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00204">
        <property type="entry name" value="dxs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00801">
        <property type="entry name" value="TRANSKETOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00802">
        <property type="entry name" value="TRANSKETOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000256446" description="1-deoxy-D-xylulose-5-phosphate synthase">
        <location>
            <begin position="1"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="144"/>
            <end position="146"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="176"/>
            <end position="177"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="396"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00315"/>
        </source>
    </evidence>
    <sequence length="666" mass="71480" checksum="2099615DC9AFF4B5" modified="2005-10-11" version="1">MNVCCVKIPACVRRFRTCQIGNVAVTDFSSTPLLDTIRTPADLRKLKVEQVRQVADELRLETIDAVSVTGGHFGAGLGVVELTTAIHYIFDTPRDRLIWDVGHQAYPHKILTGRRDRIRTLRTGGGLSGFTKRTESDYDPFGAAHSSTSISASLGMAVARDLSGGNNNVIAVIGDGAMSAGMAYEAMNNAGAMNSRLIVILNDNDMSIAPPVGAMSAYLSRLYSGKTYRSLREAAKQLGKHLPKMIADRAERVEEYSRGFMTNSGTLFEELGFYYVGPIDGHNLDHLLPVLKNVRDMENGPILVHVVTQKGKGYPPAEAAADKYHAVVKFDISTGAQSKSKPNAPSYQNVFGASLVKEAEKDDKIVAITAAMPSGTGVDIFNNAFPERTFDVGIAEQHAVTFAAGLATEGFKPFCAIYSTFLQRGYDQVVHDVAIQSLPVRFAIDRAGLVGADGATHAGSFDNAFLGCLPNMVIMAAADEAELVHMVATQVAINDRPSAVRYPRGEGRGVEMPEVGVPLPIGKGRIVRQGSKIALLSFGTRLAECEKAADELAAHGLSTTIADARFMKPLDVDLALKLAREHDVLITIEEGSIGGFGSHVMQTLMDNGALDGGLVRVRSMILPDEFLDHDTPTAMYARAGLDAKGIVAKVFEALGKDVNTETVKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV07</accession>
    <name>Q3SV07_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">AMP-dependent synthetase and ligase</fullName>
            <ecNumber evidence="4">6.2.1.26</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0617</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.26" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313944.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0617"/>
    <dbReference type="EnsemblBacteria" id="ABA03884">
        <property type="protein sequence ID" value="ABA03884"/>
        <property type="gene ID" value="Nwi_0617"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0617"/>
    <dbReference type="eggNOG" id="COG0318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_5_5"/>
    <dbReference type="OMA" id="FAIPSMG"/>
    <dbReference type="OrthoDB" id="377638at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008756">
        <property type="term" value="F:o-succinylbenzoate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="2">
        <location>
            <begin position="19"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="3">
        <location>
            <begin position="448"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="534" mass="58889" checksum="E10B637564412A8A" modified="2005-10-11" version="1">MLGLMQDWPLLCHRIIDHAARIHAGREVVTRSVEGPIHRTNYAEIRGRALKLAQRLDRQGIRLGDRVATLAWNTWRHLEAWYGIMGIGAVCHTVNPRLFPDQIAWIINHATDRIVMTDITFVPILEKIAGRLPSVERYVVLTDGAHMPRTSLKNAVAYEDWIAEADGDFAWKTFDENTAAAMCYTSGTTGDPKGVVYSHRSNVLHSMIVNNADALGAGSKDTLLPVVPLFHANSWGTAFSAPSMGTRLVMPGARLDGASVYELLSGEKVTFAAGVPTVWLMLLQHMETDNLKLPDLKVVICGGSAMPRSIIKAFDDMGIEVRHAWGMTEMSPRGTVGALQGAFSHLKGDARLDRLQMQGYAPFMVEMKLTDDAGNELPWDGKTPGRLKVRGPAISAAYYRVDDDILDEEGFFDTGDVATLDSYGYLRITDRSKDVIKSGGEWISSIDLENLAVGHPKVAEAAVIGLPHPKWGERPLLIVQVKPGESVTREEMLDFMDGKIAKWWMPDDVAFVDSIPHTATGKILKAALREQFKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV39</accession>
    <name>METXA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Homoserine O-acetyltransferase</fullName>
            <shortName evidence="1">HAT</shortName>
            <ecNumber evidence="1">2.3.1.31</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Homoserine transacetylase</fullName>
            <shortName evidence="1">HTA</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">metXA</name>
        <name type="ordered locus">Nwi_0585</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transfers an acetyl group from acetyl-CoA to L-homoserine, forming acetyl-L-homoserine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + L-homoserine = CoA + O-acetyl-L-homoserine</text>
            <dbReference type="Rhea" id="RHEA:13701"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57716"/>
            <dbReference type="EC" id="2.3.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; O-acetyl-L-homoserine from L-homoserine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. MetX family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.31" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313913.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SV39"/>
    <dbReference type="SMR" id="Q3SV39"/>
    <dbReference type="STRING" id="323098.Nwi_0585"/>
    <dbReference type="ESTHER" id="nitwn-metx">
        <property type="family name" value="Homoserine_transacetylase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03852">
        <property type="protein sequence ID" value="ABA03852"/>
        <property type="gene ID" value="Nwi_0585"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0585"/>
    <dbReference type="eggNOG" id="COG2021">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028760_1_2_5"/>
    <dbReference type="OMA" id="TRFCVVS"/>
    <dbReference type="OrthoDB" id="536745at2"/>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00074"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004414">
        <property type="term" value="F:homoserine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00296">
        <property type="entry name" value="MetX_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008220">
        <property type="entry name" value="HAT_MetX-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32268">
        <property type="entry name" value="PTHR32268"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000443">
        <property type="entry name" value="Homoser_Ac_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01392">
        <property type="entry name" value="homoserO_Ac_trn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0486">Methionine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000231875" description="Homoserine O-acetyltransferase">
        <location>
            <begin position="1"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="63"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="334"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="367"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="368"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="399" mass="43815" checksum="6E491230FD5E0793" modified="2005-10-11" version="1">MVKVQSISGPAHPEHRAQEADHPTSQVATFDTDQPLPLDCGVDLAPFQIAYQTYGKLNPAKSNAILICHALTGDQHVANPHPVTGKPGWWVTLVGPGKPLDTSRYFIICSNVIGGCMGSTGPASTNPETGRVWGLDFPVITIPDMVRAQAMLIDRLGIDTLFSVVGGSMGGMQVLQWCVAYPKRVFSALPIACSTRHSAQNIAFHELGRQAVMADPDWREGRYVEQGTHPRRGLGVARMAAHITYLSDAALHRKFGRRMQDRELPTFSFDADFQVESYLRHQGSSFVERFDANSYLYLTRAMDYFDIAADHDGVLAAAFRGTRTRFCVVSFTSDWLFPTAESRATVHALNAGGARVSFAEIETDRGHDAFLLDVPEFFDIAHAFLESAGKTRGLADAER</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV75</accession>
    <name>Q3SV75_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase, family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0549</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313877.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0549"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03816">
        <property type="protein sequence ID" value="ABA03816"/>
        <property type="gene ID" value="Nwi_0549"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0549"/>
    <dbReference type="eggNOG" id="COG1216">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_333082_0_0_5"/>
    <dbReference type="OMA" id="CRAFEIA"/>
    <dbReference type="OrthoDB" id="948440at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="245"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="818" mass="89916" checksum="CE94C9C7832E3A2B" modified="2005-10-11" version="1">MVLGPVTDNQERAERSTRVMLERMAAQALNSRDYLAAFKYADRHCRIDPSSAAHCFVLRAEANWKLERKEAAMADLAQALLVDPSDLAANRRMLAWSATGARRRTAAISLIARDNNPAVLRLAIEELRRAGHRHWAACSVFDDHVTGWIAWTLANAVEVSVGVENSALISRIEPDPFHPLASIDVQAAAFLARRPASRAPQTLTLTCDGEVFRVRRLAPNLSSPPASSNRSRRSTAPQPDTLPPTVIVPVYRDVAATLDCFDSLIKARASRAAGKRAFRIMAIDDASPEPELRRHLGELAARGAIDLLANENNLGFVGAINRALENVPAGDVVLLNSDTIVPPGFVERLADVAHSAPDIGTVTPLSNNGDMFSFPVPNRLNPMQSYDGVLEIDGIASIANAGDVTDVPSGIGFCLYVTRGCLKAIGALSEHFERGYLEDVDLCLRARDKGFRNVCAPSVYVGHHGSKSFQHEKRRLVLRNLEVLDQRFPDYRRECRAFEIADPLRPARARLDRALTWPRQPSVLILGHRRKFAVAEERVRQLRQRGERAILLSRERDVIHVRAADGSSPQAVQLKLDTETGITEAAGVITRLHPERVEVFEPSPLPRLIDLIRQLGLPVHPWLTTGTLSEAITSLPGEVPLFAPGRIAQAFAKARWPGRKIILQNWPTRPLTLKPVHRARRSLAIVPSAPSPASFRMMRRLADGLRRSAPSRSVIVAGVTCDDDRLMSCENIFVTGKVAADEIGDVLAPHNPGWLLTDFDEPAFGHPLIEAARRASIPVAYRDWSAGSVKPRKADLAIPSDTDEEEFIDAVIAWAGLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV93</accession>
    <name>Q3SV93_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0531</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313859.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0531"/>
    <dbReference type="EnsemblBacteria" id="ABA03798">
        <property type="protein sequence ID" value="ABA03798"/>
        <property type="gene ID" value="Nwi_0531"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0531"/>
    <dbReference type="eggNOG" id="COG2963">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143474_0_0_5"/>
    <dbReference type="OMA" id="GTEGPRQ"/>
    <dbReference type="OrthoDB" id="1839196at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="72"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="131" mass="14578" checksum="56BD7E69B066824E" modified="2005-10-11" version="1">MKKHSREEILSKLARADELARAGNSQVDVCKALGVSVMTLHRWRKLPLPRQEADIPHDNAGEGAAFVKPPAMDQMRHVLEELKLENQRLRKIVTDLLLEKMRLEEAAVSASPPQSETQGVIQALLGPANSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVD1</accession>
    <name>Q3SVD1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ferric uptake regulation protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0493</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as a global negative controlling element, employing Fe(2+) as a cofactor to bind the operator of the repressed genes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Fur family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0493"/>
    <dbReference type="EnsemblBacteria" id="ABA03760">
        <property type="protein sequence ID" value="ABA03760"/>
        <property type="gene ID" value="Nwi_0493"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0493"/>
    <dbReference type="eggNOG" id="COG0735">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096072_2_0_5"/>
    <dbReference type="OMA" id="QHELTTF"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07153">
        <property type="entry name" value="Fur_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002481">
        <property type="entry name" value="FUR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043135">
        <property type="entry name" value="Fur_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33202">
        <property type="entry name" value="PTHR33202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01475">
        <property type="entry name" value="FUR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="177" mass="19082" checksum="16B9AB2D1F844D63" modified="2005-10-11" version="1">MGSGTGSNGLMDNRMTATRPTFPVPGHDHDRCAAEAISHAEQICARRGQKFTPIRRQVLQALLSSHRPLGAYEVIEELAKSIPRPAPITVYRALDFLMGNGLVHRIESRNAFLACGHDHDESAMVAFLICEQCGSVGEIPASRVARSLSDAVRTTGFTPKLSVVEITGTCAHCQNAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVD6</accession>
    <name>Q3SVD6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_0488</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NodD regulates the expression of the nodABCFE genes which encode other nodulation proteins. NodD is also a negative regulator of its own expression. Binds flavonoids as inducers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313816.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0488"/>
    <dbReference type="EnsemblBacteria" id="ABA03755">
        <property type="protein sequence ID" value="ABA03755"/>
        <property type="gene ID" value="Nwi_0488"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0488"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_16_3_5"/>
    <dbReference type="OMA" id="RFDVQHL"/>
    <dbReference type="OrthoDB" id="750230at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0536">Nodulation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA03755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="309" mass="34346" checksum="6CD98A19431F451A" modified="2005-10-11" version="1">MDRIDLFRVFVRVVELASFTRAADALVLPRSSVSAAVRELESRVGARLLHRTTRKVVPTPDGTAFYKRCLRLLADVEETEALFRQSARRPVGKLKVDVPGRLGRLIIVPALPDFFARYPDIELDLGVTDRAVNLVEQSVDGVLRVGVLSQSGLIARSVGVLPLINVAAPSYLGRHGIPETPDDLDTHLAVRYASPSTGRIEDWEWIDNGHVRTRVVAGRITVNSAEAYIACALAGLGMIQIPAYDVKPHLAAGELVEILPHHRAQPFPMTFLYPSRQHRSRRVQVFADWLENLLRERVLHLDPTQALSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVF2</accession>
    <name>Q3SVF2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Uroporphyrinogen-III synthase</fullName>
            <ecNumber evidence="1">4.2.1.75</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0472</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>hydroxymethylbilane = H2O + uroporphyrinogen III</text>
            <dbReference type="Rhea" id="RHEA:18965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57308"/>
            <dbReference type="ChEBI" id="CHEBI:57845"/>
            <dbReference type="EC" id="4.2.1.75"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 3/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the uroporphyrinogen-III synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.75" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313803.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0472"/>
    <dbReference type="EnsemblBacteria" id="ABA03739">
        <property type="protein sequence ID" value="ABA03739"/>
        <property type="gene ID" value="Nwi_0472"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0472"/>
    <dbReference type="eggNOG" id="COG1587">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011276_10_1_5"/>
    <dbReference type="OMA" id="LAIPQCC"/>
    <dbReference type="OrthoDB" id="1185397at2"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00320"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004852">
        <property type="term" value="F:uroporphyrinogen-III synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006780">
        <property type="term" value="P:uroporphyrinogen III biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06578">
        <property type="entry name" value="HemD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10090">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036108">
        <property type="entry name" value="4pyrrol_syn_uPrphyn_synt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003754">
        <property type="entry name" value="4pyrrol_synth_uPrphyn_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039793">
        <property type="entry name" value="UROS/Hem4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38042">
        <property type="entry name" value="PTHR38042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02602">
        <property type="entry name" value="HEM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69618">
        <property type="entry name" value="SSF69618"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEM4" evidence="2">
        <location>
            <begin position="14"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="248" mass="25789" checksum="B33DFBA12E182FC7" modified="2005-10-11" version="1">MAVLVTRPEPDNETTASALRAMGREALLSPVLRFEAAAFEIDRGANYGAVIVTSANALRALAVHPGIESLLKLAVFAVGRHTAEAARRVGFQDVVAAAGDAAALCELIVKRVRAKSLPKGARILYLAAADRSCDLAGKLEGHGLAVVTTTCYRMAPVASLSREVCDAFAAHRISAVLHYSRRSARAFLDAVRIGGVEISALAVPQCCISGAVASVLRDAGAPHVVEASAPAEEAVLEALDCALRPASQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVG1</accession>
    <name>Q3SVG1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6460 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0463</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313794.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0463"/>
    <dbReference type="EnsemblBacteria" id="ABA03730">
        <property type="protein sequence ID" value="ABA03730"/>
        <property type="gene ID" value="Nwi_0463"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0463"/>
    <dbReference type="eggNOG" id="ENOG5032ZW8">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_167396_0_0_5"/>
    <dbReference type="OMA" id="VMTVFDW"/>
    <dbReference type="OrthoDB" id="2075423at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045594">
        <property type="entry name" value="DUF6460"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20061">
        <property type="entry name" value="DUF6460"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="DUF6460" evidence="2">
        <location>
            <begin position="63"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="101" mass="11323" checksum="84E9068D4CA536BF" modified="2005-10-11" version="1">MPNDDTRNFRASHDSLSRFLGGSPLAVAFRLILLSTLVGVMLAAIGFDPWNIVQSIRLLVERVWDLGFDAINGLWRYFLLGAVIVIPIWLLSRLFGAPRGR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-08-03" version="127" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVJ1</accession>
    <name>ATPB_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP synthase subunit beta</fullName>
            <ecNumber evidence="1">7.1.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP synthase F1 sector subunit beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">F-ATPase subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpD</name>
        <name type="ordered locus">Nwi_0433</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Produces ATP from ADP in the presence of a proton gradient across the membrane. The catalytic sites are hosted primarily by the beta subunits.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + 4 H(+)(in) + H2O = ADP + 5 H(+)(out) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a(1), b(2) and c(9-12). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. CF(1) is attached to CF(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313764.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVJ1"/>
    <dbReference type="SMR" id="Q3SVJ1"/>
    <dbReference type="STRING" id="323098.Nwi_0433"/>
    <dbReference type="EnsemblBacteria" id="ABA03700">
        <property type="protein sequence ID" value="ABA03700"/>
        <property type="gene ID" value="Nwi_0433"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0433"/>
    <dbReference type="eggNOG" id="COG0055">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022398_0_2_5"/>
    <dbReference type="OMA" id="GFNMIMD"/>
    <dbReference type="OrthoDB" id="430176at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045261">
        <property type="term" value="C:proton-transporting ATP synthase complex, catalytic core F(1)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01347">
        <property type="entry name" value="ATP_synth_beta_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005722">
        <property type="entry name" value="ATP_synth_F1_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="SSF50615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01039">
        <property type="entry name" value="atpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0139">CF(1)</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000254316" description="ATP synthase subunit beta">
        <location>
            <begin position="1"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="154"/>
            <end position="161"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01347"/>
        </source>
    </evidence>
    <sequence length="476" mass="50823" checksum="F400501C1B9A0FDF" modified="2005-10-11" version="1">MATAANQVGRITQVIGAVVDVQFEGHLPAILNAIETKNGENRLVLEVAQHLGESTVRTIAMDTTEGLVRGQDVTDTGAPIRVPVGAGTLGRIMNVIGEPVDEQGPVKADDLRPIHAEAPSYTDQSTEAEILVTGIKVVDLLAPYAKGGKIGLFGGAGVGKTVLIQELINNVAKAHGGYSVFAGVGERTREGNDLYHEFIESGVNKKGGGEGSKCALVYGQMNEPPGARARVALSGLTVAEHFRDQGQDVLFFVDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATDMGALQERITTTNKGSITSVQAIYVPADDLTDPAPATSFAHLDATTVLNRAISEKGIYPAVDPLDSTSRMLSPLIVGEEHYQTARLVQQVLQRYKSLQDIIAILGMDELSEEDKLTVARARKVERFLSQPFHVAEVFTGSPGKFVDLADTIKGFRDLCQGKYDHLPEAAFYMVGTIEEAVEKGKKLAAEAA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVK5</accession>
    <name>MDH_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Malate dehydrogenase</fullName>
            <ecNumber evidence="1">1.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mdh</name>
        <name type="ordered locus">Nwi_0419</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible oxidation of malate to oxaloacetate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-malate + NAD(+) = H(+) + NADH + oxaloacetate</text>
            <dbReference type="Rhea" id="RHEA:21432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.37"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH/MDH superfamily. MDH type 3 family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.37" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313750.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVK5"/>
    <dbReference type="SMR" id="Q3SVK5"/>
    <dbReference type="STRING" id="323098.Nwi_0419"/>
    <dbReference type="EnsemblBacteria" id="ABA03686">
        <property type="protein sequence ID" value="ABA03686"/>
        <property type="gene ID" value="Nwi_0419"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0419"/>
    <dbReference type="eggNOG" id="COG0039">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045401_2_1_5"/>
    <dbReference type="OMA" id="MGWTSQA"/>
    <dbReference type="OrthoDB" id="870724at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030060">
        <property type="term" value="F:L-malate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01339">
        <property type="entry name" value="LDH-like_MDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00487">
        <property type="entry name" value="Malate_dehydrog_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011275">
        <property type="entry name" value="Malate_DH_type3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00086">
        <property type="entry name" value="LLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="SSF56327"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01763">
        <property type="entry name" value="MalateDH_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="chain" id="PRO_0000241954" description="Malate dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="119"/>
            <end position="121"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00487"/>
        </source>
    </evidence>
    <sequence length="322" mass="34061" checksum="D35B3292E950CC85" modified="2005-10-11" version="1">MARDKIGLIGSGQIGGTLAHLIGLKELGDVVMFDIADGVPQGKSLDIAQSSPVEGFDARLAGTNSYEALEGAGVCIVTAGVPRKPGMSRDDLLGINLKVMEQVGAGIRKYAPDAFVICITNPLDAMVWALQKASGLPAKKVVGMAGVLDSSRFRYFLADEFNVSVEDVTAFVLGGHGDSMVPLVKYSTVAGIPLPDLVKMGWTSQARIDEIVDRTRNGGAEIVNLLKTGSAFYAPAASAIAMAESFLRDKKRVLPCAAYLNGEFGVYDMYVGVPVVIGAKGVERIVEIELAGRDREAFDRSVAAVQGLVDACKKIAPDLLDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVN0</accession>
    <name>Q3SVN0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0394</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313725.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0394"/>
    <dbReference type="EnsemblBacteria" id="ABA03661">
        <property type="protein sequence ID" value="ABA03661"/>
        <property type="gene ID" value="Nwi_0394"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0394"/>
    <dbReference type="eggNOG" id="ENOG5033A7M">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_191964_0_1_5"/>
    <dbReference type="OrthoDB" id="2048223at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="48" mass="5274" checksum="D23D0E4A5DC0D025" modified="2005-10-11" version="1">MPSLFRFLTIVAALGAIAYGVILGLANFVEPTPREMTVTVHPDKFLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW02</accession>
    <name>Q3SW02_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Phage integrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_0272</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313605.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0272"/>
    <dbReference type="EnsemblBacteria" id="ABA03539">
        <property type="protein sequence ID" value="ABA03539"/>
        <property type="gene ID" value="Nwi_0272"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0272"/>
    <dbReference type="eggNOG" id="COG0582">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_676050_0_0_5"/>
    <dbReference type="OMA" id="LSLWWED"/>
    <dbReference type="OrthoDB" id="785253at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="7">
        <location>
            <begin position="60"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="6">
        <location>
            <begin position="169"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="393"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA03539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="424" mass="48231" checksum="9DFC416745F087E7" modified="2005-10-11" version="1">MSDIRKRVGRKGTTYQVRYPSKSSKTGYAYKTFATRKEALAFRDDSAARASAARPQSDSRTVEQGVQKWLDVCEKEGRNGREPVTKYTLANYRYRADIMLSYPWDKQLHELTKPDIIEFRSWLLQKHSRDVAQKVLLSFHSMIRELVGRGILQNDMAAGVSIAAASRYDTSVTIPSEADIMALLAAADRLANARNLKVQQSWQRYRPMLYLAVDSGMRPQEYLVLARSAIKETGVEVSRALEGGGTAITVTKTRAGRRFIDLSAETLDMVRHYAEHHAVANRYDLVFPTETGKWLSIRNWRNRAFARACEEAGLMVEEEENGEKVMRPKYRPYDLRHFYASMLIEQRVNLKRIQTLMGHRNIQTTLNVYGHLIERAEAVADKRTSVLSFVERDASTAPADGVKSRSRPRKRKAASDASFLEAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW52</accession>
    <name>Q3SW52_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_0221</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase U62 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313556.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0221"/>
    <dbReference type="EnsemblBacteria" id="ABA03489">
        <property type="protein sequence ID" value="ABA03489"/>
        <property type="gene ID" value="Nwi_0221"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0221"/>
    <dbReference type="eggNOG" id="COG0312">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026425_1_0_5"/>
    <dbReference type="OMA" id="TDFWGSM"/>
    <dbReference type="OrthoDB" id="806701at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045570">
        <property type="entry name" value="Metalloprtase-TldD/E_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002510">
        <property type="entry name" value="Metalloprtase-TldD/E_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025502">
        <property type="entry name" value="TldD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035068">
        <property type="entry name" value="TldD/PmbA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01523">
        <property type="entry name" value="PmbA_TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19290">
        <property type="entry name" value="PmbA_TldD_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004919">
        <property type="entry name" value="TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="SSF111283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PmbA_TldD" evidence="3">
        <location>
            <begin position="35"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="PmbA_TldD_M" evidence="5">
        <location>
            <begin position="122"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="PmbA_TldD_C" evidence="4">
        <location>
            <begin position="239"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF19289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF19290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA03489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="475" mass="50636" checksum="B0ED8C1FC05FC6BE" modified="2005-10-11" version="1">MTSPITASLLDRAHLDRDSVRHEIARGLRGADDGELFLEYSQSEALMFDNGRLKQATYDTSQGFGLRAVKDDAVGYAHSSDVSLAALIRAADAVGAVRGGYQGAFAVAPGHTNVRLYGDDNPLDGLGFETKVKLLAEIDAYVRDRDPRVRQVSVSLGATWQVVEIMRPDGETYRDIRPLVRVNISVVAGQGDRQETGSKGYGGREGYARFVESKAWREVADGAIRQALINLESIPAPAGEMDVVLGPGWPGVMLHEAVGHGLEGDFNRKKTSAFAGLMGQQVAAKGVTVVDDGTISARRGSLSIDDEGTPTSRTVLIEDGILVGYMQDRQNARLMGMKPTGNGRRQDYAHVPMPRMTNTYMPAGDRDPAEILASVKNGIYAVNFGGGQVDITSGKYVFQCTEAYRIENGKVGAPLKGAMLIGNGPTDLHRITMIGNDVALDDGVGTCGKNGQGVPVGVGQPTLRMERITVGGTGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW54</accession>
    <name>Q3SW54_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Pirin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0219</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the pirin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313554.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03487">
        <property type="protein sequence ID" value="ABA03487"/>
        <property type="gene ID" value="Nwi_0219"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0219"/>
    <dbReference type="eggNOG" id="COG1741">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045717_1_1_5"/>
    <dbReference type="OMA" id="RPHPHIN"/>
    <dbReference type="OrthoDB" id="833478at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012093">
        <property type="entry name" value="Pirin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008778">
        <property type="entry name" value="Pirin_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003829">
        <property type="entry name" value="Pirin_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903">
        <property type="entry name" value="PTHR13903"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02678">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05726">
        <property type="entry name" value="Pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006232">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pirin" evidence="3">
        <location>
            <begin position="35"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Pirin_C" evidence="4">
        <location>
            <begin position="193"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="306" mass="33822" checksum="188FC64271D33266" modified="2005-10-11" version="1">MSWLPSNDPALGDPQSCDALELVIIPRVRDLGDGFAVRRALPHGRRQMVGPFIFFDHFGPIQFVSGKGMDVRPHPHIGLATVTYLFDGSIMHRDSEGHIQEIQPGAMNLMTAGSGIAHSERTPDAQRASGQAMLGLQSWIALPKGKEEIAPTFQHYGADSLPTVQDKGFRARVIAGRSFGATSPVEMVSPWFYAEVALEAGMSAPLDADHEERAIYIVDGEVEIAGDRHQAPQLLVFRPGDRITVRATQPTRMMFLGGDALEGPRHIWWNFVSSSKERIEQAKEDWKTGRFAPVPNEHEFIPLPEN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-21" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW72</accession>
    <name>DAPB_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4-hydroxy-tetrahydrodipicolinate reductase</fullName>
            <shortName evidence="1">HTPA reductase</shortName>
            <ecNumber evidence="1">1.17.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dapB</name>
        <name type="ordered locus">Nwi_0201</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-2,3,4,5-tetrahydrodipicolinate + H2O + NAD(+) = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:35323"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="EC" id="1.17.1.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-2,3,4,5-tetrahydrodipicolinate + H2O + NADP(+) = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:35331"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="EC" id="1.17.1.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 4/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DapB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Was originally thought to be a dihydrodipicolinate reductase (DHDPR), catalyzing the conversion of dihydrodipicolinate to tetrahydrodipicolinate. However, it was shown in E.coli that the substrate of the enzymatic reaction is not dihydrodipicolinate (DHDP) but in fact (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid (HTPA), the product released by the DapA-catalyzed reaction.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="ABA03469" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="1.17.1.8" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03469.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SW72"/>
    <dbReference type="SMR" id="Q3SW72"/>
    <dbReference type="STRING" id="323098.Nwi_0201"/>
    <dbReference type="EnsemblBacteria" id="ABA03469">
        <property type="protein sequence ID" value="ABA03469"/>
        <property type="gene ID" value="Nwi_0201"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0201"/>
    <dbReference type="eggNOG" id="COG0289">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047479_2_1_5"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00018"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008839">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate reductase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016726">
        <property type="term" value="F:oxidoreductase activity, acting on CH or CH2 groups, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00102">
        <property type="entry name" value="DapB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022663">
        <property type="entry name" value="DapB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000846">
        <property type="entry name" value="DapB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022664">
        <property type="entry name" value="DapB_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023940">
        <property type="entry name" value="DHDPR_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20836">
        <property type="entry name" value="PTHR20836"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05173">
        <property type="entry name" value="DapB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01113">
        <property type="entry name" value="DapB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000161">
        <property type="entry name" value="DHPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00036">
        <property type="entry name" value="dapB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01298">
        <property type="entry name" value="DAPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword id="KW-0457">Lysine biosynthesis</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000228365" description="4-hydroxy-tetrahydrodipicolinate reductase">
        <location>
            <begin position="1"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="97"/>
            <end position="99"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="121"/>
            <end position="124"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>(S)-2,3,4,5-tetrahydrodipicolinate</name>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="164"/>
            <end position="165"/>
        </location>
        <ligand>
            <name>(S)-2,3,4,5-tetrahydrodipicolinate</name>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="267" mass="27403" checksum="AFB0962FFFC2BC59" modified="2006-03-21" version="2">MRLIVAGAGGRMGRALVRAIAETDGAVLAGALEAPESELIGKDASVLAGLPASGIEVSADLWSLSAKADGILDFTVPAATIANVAVAAERGLIHVIGTTGLSASDMAVIRSVTSRTAVVQSGNMSLGVNLLAALVKRMAQALDESFDVEVLEMHHRAKIDAPSGTALMLGQAAADGRKVGLDQRAVRGRDGLTGARKAGDIGFASLRGGTVSGDHSVIFAGPYERITLSHHAEDRMIFAHGALKAAKWAFGKEPGLYSMSDVLGLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW85</accession>
    <name>Q3SW85_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Possible outer membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0188</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313524.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0188"/>
    <dbReference type="EnsemblBacteria" id="ABA03456">
        <property type="protein sequence ID" value="ABA03456"/>
        <property type="gene ID" value="Nwi_0188"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0188"/>
    <dbReference type="eggNOG" id="COG3637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_609535_0_0_5"/>
    <dbReference type="OMA" id="KSHRDGW"/>
    <dbReference type="OrthoDB" id="2018542at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036709">
        <property type="entry name" value="Autotransporte_beta_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027385">
        <property type="entry name" value="OMP_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13505">
        <property type="entry name" value="OMP_b-brl"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103515">
        <property type="entry name" value="SSF103515"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229195" evidence="1">
        <location>
            <begin position="20"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="OMP_b-brl" evidence="2">
        <location>
            <begin position="9"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="OMP_b-brl" evidence="2">
        <location>
            <begin position="236"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="453" mass="48062" checksum="76EF0E6860D426D3" modified="2005-10-11" version="1">MKKILLGVLLASTAMSAQAADLAPRPYTKAPPIAPVYDWTGLYVGVNAGLGLGRNRAVHDFGVGAISTHLQPLGAVGGGQIGYNWQTNSFFGPLVFGIEADIQGADMTDNRTNFAGLLQYNQRLDWFGTARGRVGLINGPMLTYVTGGWAYGHVGTTVIQGGVPFAFSGGRNGWTFGSGVEAALGGNWTGKIEYLYLDFGNRTDSFAGQALRSEMRENIFRAGLNYRFGASNPYAPVATANWNGLYLGGNVGGGVGRNRTTISNPAGNLAQFNLAPDGFVGGGQIGYNWQAGNIVLGLETDFQGATLQDNKTCIGCVPGASFDFNSKLQWFGTVRGRLGYAVGNSMFYATGGFAYGNIRTALNTPLNNFNFSNTRTGYAVGGGIETPLPMVNLFGGPLFGPNWTSTTEYLFVDLGRSSFNIGGGPNVLTTGAQEHIFRTGLNYHFNIPLAPSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWB3</accession>
    <name>Q3SWB3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0160</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313497.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0160"/>
    <dbReference type="EnsemblBacteria" id="ABA03428">
        <property type="protein sequence ID" value="ABA03428"/>
        <property type="gene ID" value="Nwi_0160"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0160"/>
    <dbReference type="eggNOG" id="COG2270">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017518_3_1_5"/>
    <dbReference type="OMA" id="YAQEVMG"/>
    <dbReference type="OrthoDB" id="1249292at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024671">
        <property type="entry name" value="Atg22-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11700">
        <property type="entry name" value="ATG22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="264"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="295"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="329"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="375"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="410"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="441"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="1"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="462" mass="48421" checksum="B77ED4EA3BD5DB6B" modified="2005-10-11" version="1">MAVTTALPADGAEPGERYASRTAINSWIMFDWAAQPYFTLITTFVFAPYFATFVAATPAEGQSLWGFATGAAGLVIALASPVLGAIADANGRRKPWIAAFGALLVIGSSLMWIGKPGDPGVILPLLIAYGIATIGVEFATVFNNAMMPSLVPPHRIGRLSGTGWATGYVGGILSLILVLGFLAANPATGKTLFGMTPLFGLDPATHEGDRITGPLTGLWFIIFVLPMFLLTPDLPAKRSVRMALREGLIGLRGTLRELPQRRSLATFLLANMIYTDGLVSLFAFGGIYAAGTFGWHTIEIGTFGMLLAIAGALGAWIGGKLDDRVGPKAVIATSMTVLLLSIIMILMVDKDSILFVSVTPPSPGGGLFAAPAEKAYLILGCLIGGAGGPLQAASRTLLIRMAPKERITQFFGLFALTGKVTSFVGPLLIGFITAVTASQKAGMAVLVLFFVAGLALLSRVRD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWH0</accession>
    <name>NTPP_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleoside triphosphate pyrophosphatase</fullName>
            <ecNumber evidence="1">3.6.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Nucleotide pyrophosphatase</fullName>
            <shortName evidence="1">Nucleotide PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">Nwi_0103</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Nucleoside triphosphate pyrophosphatase. May have a dual role in cell division arrest and in preventing the incorporation of modified nucleotides into cellular nucleic acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-phosphate + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23996"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="EC" id="3.6.1.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + H2O = a 2'-deoxyribonucleoside 5'-phosphate + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44644"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:65317"/>
            <dbReference type="EC" id="3.6.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Maf family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.9" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313440.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWH0"/>
    <dbReference type="SMR" id="Q3SWH0"/>
    <dbReference type="STRING" id="323098.Nwi_0103"/>
    <dbReference type="EnsemblBacteria" id="ABA03371">
        <property type="protein sequence ID" value="ABA03371"/>
        <property type="gene ID" value="Nwi_0103"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0103"/>
    <dbReference type="eggNOG" id="COG0424">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040416_1_1_5"/>
    <dbReference type="OMA" id="CAGSFKA"/>
    <dbReference type="OrthoDB" id="1469203at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036218">
        <property type="term" value="F:dTTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035529">
        <property type="term" value="F:NADH pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00555">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00528">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003697">
        <property type="entry name" value="Maf-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43213">
        <property type="entry name" value="PTHR43213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02545">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006305">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="SSF52972"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000267353" description="Nucleoside triphosphate pyrophosphatase">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00528"/>
        </source>
    </evidence>
    <sequence length="202" mass="21719" checksum="E9B629B4C5E4670A" modified="2005-10-11" version="1">MAIWRGSEPLILASQSRVRQLLLANAGLPFETMPAAIDERAVQRSSGLTTAGEIAVRLACEKAREVSSRSPGRYVIGADQTLECDGRLLNKPAGRADAGAHLRALSGRTHALHAGVAVVRDGRLMFENVSVARMTMRELSEDTIESYLDAAGDAASASVGAYQLEGLGIHLFSRIEGDHFTILGLPLLPLLAYLRSQWLLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWM7</accession>
    <name>Q3SWM7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA threonylcarbamoyladenosine biosynthesis protein TsaE</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_0046</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TsaE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313385.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0046"/>
    <dbReference type="EnsemblBacteria" id="ABA03314">
        <property type="protein sequence ID" value="ABA03314"/>
        <property type="gene ID" value="Nwi_0046"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0046"/>
    <dbReference type="eggNOG" id="COG0802">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3178">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021467_2_1_5"/>
    <dbReference type="OMA" id="SPNIIWR"/>
    <dbReference type="OrthoDB" id="443861at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012180">
        <property type="entry name" value="Bifunc_ATPase/PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003442">
        <property type="entry name" value="T6A_TsaE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02367">
        <property type="entry name" value="TsaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036599">
        <property type="entry name" value="AtpPhos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00150">
        <property type="entry name" value="T6A_YjeE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="APH" evidence="5">
        <location>
            <begin position="176"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00032441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA03314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="507" mass="55225" checksum="7B34533DDBAD9C41" modified="2005-10-11" version="1">MSEPSRFATALVNETATAHLMADLALLIGPGDVITLSGDLGAGKTAAARALIRYLAGDDTIEVPSPTFTLAQHYDLPPHPLLHADLYRISGPGELDEIGLAPLPEDAVVLIEWPERAAGGLPADRIDIAISHRPALGSAARSAEITGYGKAASQVARLASLRRFLDDAGYLDARRQRMAGDASTRSYARLIRDDASFILMNAPKRPDGPAIHDGKSYSAAVHLAEDIKPFVAIAHGLRGQGFSAPEIHHADLESGFLITEDFGSAGFVEGTPPAPIVERYQAATDLLAALHGRTLPDTLPLTADTAYAIPAFDLEAMLIEVGLMLDWYLIDRGIEPDEGLRAEFLAIWRALLAKPDAASRTWVLRDFHSPNLIWLEQRRDIGKVGIIDFQDTVLGPDAYDLVSLLQDARVDVPESIELALLARYAKARLECEAHFDPAGFAETYAIISAQRNTRLLGTFARLNRRDGKPHYLRHQPRIWTYLTRSLAHPALSAARAWYRNHVPPPAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMW1</accession>
    <name>Q3SMW1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulator, MerR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_3133</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316288.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3133"/>
    <dbReference type="EnsemblBacteria" id="ABA06380">
        <property type="protein sequence ID" value="ABA06380"/>
        <property type="gene ID" value="Nwi_3133"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3133"/>
    <dbReference type="eggNOG" id="COG0789">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060077_2_0_5"/>
    <dbReference type="OMA" id="FIKCMRN"/>
    <dbReference type="OrthoDB" id="1748559at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015358">
        <property type="entry name" value="Tscrpt_reg_MerR_DNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00376">
        <property type="entry name" value="MerR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09278">
        <property type="entry name" value="MerR-DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00040">
        <property type="entry name" value="HTHMERR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH merR-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="89"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA06380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="139" mass="15645" checksum="391885A100DA8A85" modified="2005-10-11" version="1">MTGDFLAIGELGRRTDTKIETIRYYERIGLLAAPGRTAGNYRAYGPEHLNRLSFIRRSRNLGFSLDQVRALLDLADQHDRSCDAVDAIAKEHRAEVDRKIADLRTLRRELDSMIDQCRCGTVADCRIIEALSPTTIVAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMX1</accession>
    <name>Q3SMX1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, ArsR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_3123</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316278.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3123"/>
    <dbReference type="EnsemblBacteria" id="ABA06370">
        <property type="protein sequence ID" value="ABA06370"/>
        <property type="gene ID" value="Nwi_3123"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3123"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097806_2_0_5"/>
    <dbReference type="OMA" id="QETRLHI"/>
    <dbReference type="OrthoDB" id="1800563at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="120" mass="12914" checksum="83F1C359A893D81D" modified="2005-10-11" version="1">MYEKQALDAFAALSQETRLRIVRLLVKAGPEGMSAGAIGEAMDSASSSRMSFHLSHLEHAGLVESRREGRSIIYSAALVTLSSLVEFLMRDCCQGHPEVCNPAVAALSACCEPKKRGPHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN49</accession>
    <name>Q3SN49_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_3042</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3042"/>
    <dbReference type="ESTHER" id="nitwn-q3sn49">
        <property type="family name" value="Duf_900"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06292">
        <property type="protein sequence ID" value="ABA06292"/>
        <property type="gene ID" value="Nwi_3042"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3042"/>
    <dbReference type="eggNOG" id="COG4782">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030170_1_0_5"/>
    <dbReference type="OMA" id="VGQVQWP"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010297">
        <property type="entry name" value="DUF900_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014586">
        <property type="entry name" value="UCP033909"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05990">
        <property type="entry name" value="DUF900"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF033909">
        <property type="entry name" value="UCP033909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="345" mass="36563" checksum="0952EF897AF7D28F" modified="2005-10-11" version="1">MPHHIHQPPLACRRAVLRAFAGAACTLTLGASVGGCTSAALSSGGASFDAAELTANPTLLVATTRKPVDGARAKPWYGSERAGRLHVARARLTPPGEGRFSLSAVGLGDWRLDAIEPTSQIGDLYAEPGGTRDVLIYVHGFNQTFESAALDAARISEGIRFRGETMVFSWPSRASLLDYVYDRESAMWSRDALDQVVTGLMASPITGRIHIVAHSVGTMLTMEALRQICARQPAAGDRIGAVIFASPDIDIDVFTSSVARVGPLARKITVVTATNDRALALSGFLAGGVTRVGAAEKAKLERLGLRVIDASAQGWGIINHDLFLSNAKVRQVIRRSIDGQPVDGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN52</accession>
    <name>Q3SN52_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Secretion protein HlyD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_3039</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316205.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3039"/>
    <dbReference type="EnsemblBacteria" id="ABA06289">
        <property type="protein sequence ID" value="ABA06289"/>
        <property type="gene ID" value="Nwi_3039"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3039"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018816_7_0_5"/>
    <dbReference type="OMA" id="VGQVPTR"/>
    <dbReference type="OrthoDB" id="1432684at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006143">
        <property type="entry name" value="RND_pump_MFP"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01730">
        <property type="entry name" value="RND_mfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="301" mass="30793" checksum="0B464A8A6B04226D" modified="2005-10-11" version="1">MSTKLLGGRHIATRGLALAAVWVTALVAPAGVLADEQDIPNAAAVSVLKATRFCFSASVQVTGILIPRNETAVRPERPGLKVAEILAEPGQSVTAGQALARLTRPEGGTTQISSPVAGLVSRSSAGIGAVASGKGEALFSIVTGNEFDLVGQVPSGDLAKLAVGQAAKVMAVGADEMDGKVRLIAPTVEPNSQLGQVFISVDPSKHLLVNAFGRAIIKTGESCGIAVPLTAILYGSAGAVVQVVRRQRVETRHVEVGLLSGGQVEIREGLSEGDVVVARAGALLREGDPVRPIALEESAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN99</accession>
    <name>Q3SN99_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Thioesterase superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2992</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the acyl coenzyme A hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2992"/>
    <dbReference type="EnsemblBacteria" id="ABA06242">
        <property type="protein sequence ID" value="ABA06242"/>
        <property type="gene ID" value="Nwi_2992"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2992"/>
    <dbReference type="eggNOG" id="COG1607">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050164_2_0_5"/>
    <dbReference type="OMA" id="MDEMAFL"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016790">
        <property type="term" value="F:thiolester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040170">
        <property type="entry name" value="Cytosol_ACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033120">
        <property type="entry name" value="HOTDOG_ACOT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11049">
        <property type="entry name" value="PTHR11049"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51770">
        <property type="entry name" value="HOTDOG_ACOT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HotDog ACOT-type" evidence="4">
        <location>
            <begin position="22"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA06242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="149" mass="16149" checksum="B6353E361BEEA242" modified="2005-10-11" version="1">MTDTAMTDPTAAHARPIADTAPLGELCIRTLAMPADTNQNGDIFGGWLLSQMDIGGGVFASKLAKSRTVTVAIEAMNFRKPVYVGDLVSVHATLIRVGRTSIAVHLEAWVMRRKEKQFILVTDGKFTYVAIDDNGRPREIPQTGLPNPA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-12-20" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNB8</accession>
    <name>GLMM_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoglucosamine mutase</fullName>
            <ecNumber evidence="1">5.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">glmM</name>
        <name type="ordered locus">Nwi_2973</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of glucosamine-6-phosphate to glucosamine-1-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-glucosamine 1-phosphate = D-glucosamine 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:23424"/>
            <dbReference type="ChEBI" id="CHEBI:58516"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="EC" id="5.4.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Activated by phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.10" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316145.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SNB8"/>
    <dbReference type="SMR" id="Q3SNB8"/>
    <dbReference type="STRING" id="323098.Nwi_2973"/>
    <dbReference type="EnsemblBacteria" id="ABA06223">
        <property type="protein sequence ID" value="ABA06223"/>
        <property type="gene ID" value="Nwi_2973"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2973"/>
    <dbReference type="eggNOG" id="COG1109">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016950_7_0_5"/>
    <dbReference type="OMA" id="PHNPEPL"/>
    <dbReference type="OrthoDB" id="1265792at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008966">
        <property type="term" value="F:phosphoglucosamine mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05802">
        <property type="entry name" value="GlmM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01554_B">
        <property type="entry name" value="GlmM_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006352">
        <property type="entry name" value="GlmM_bact"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="SSF53738"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="SSF55957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01455">
        <property type="entry name" value="glmM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000147923" description="Phosphoglucosamine mutase">
        <location>
            <begin position="1"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="1">
        <location>
            <position position="101"/>
        </location>
    </feature>
    <feature type="binding site" description="via phosphate group" evidence="1">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="1">
        <location>
            <position position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01554"/>
        </source>
    </evidence>
    <sequence length="448" mass="48976" checksum="991AAD732E54F815" modified="2005-10-11" version="1">MSRRYFGTDGIRGRANGLITPELAMKVGQAAGLVFQRGEHRHRVVIGKDTRLSGYMIEYALVAGFTSVGMDVLLLGPMPTPAVAMLTKSMRADLGVMISASHNLFEDNGIKMFGPRGFKLSDEVEKKIEKLLDENLDKKLAQSRNLGRARRIDGVHDRYIEFAKRTLPRELSLEGLRVVVDCAHGAAYKVVPEALWELGADVVPIGVEPDGLNINKECGSTAPEALCRKVREMRGDIGIALDGDADRVIIVDERGHVVDGDQLLAVIAESWKEDGRLTKPGIVATVMSNLGLERFLKDRDLSLVRTPVGDRYVLEQMLKQGYNLGGEQSGHIILSDYATTGDGFVSALQVLAVVQKLGRPVSQVCHKFEPLPQILKNFRYRNGKPLDRAEVKSAITAGEKRLNGHGRLLVRSSGTEPVIRVMGEGDDRILVEEVVDTIVSALGNPAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNG3</accession>
    <name>Q3SNG3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribulose bisphosphate carboxylase small subunit</fullName>
            <shortName evidence="3">RuBisCO small subunit</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cbbS</name>
        <name evidence="5" type="ordered locus">Nwi_2928</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site. Although the small subunit is not catalytic it is essential for maximal activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterohexadecamer of 8 large and 8 small subunits.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="3">The basic functional RuBisCO is composed of a large chain homodimer in a 'head-to-tail' conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric 'cap' on each end of the 'barrel'.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RuBisCO small chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316103.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2928"/>
    <dbReference type="EnsemblBacteria" id="ABA06178">
        <property type="protein sequence ID" value="ABA06178"/>
        <property type="gene ID" value="Nwi_2928"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2928"/>
    <dbReference type="eggNOG" id="COG4451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098114_2_0_5"/>
    <dbReference type="OMA" id="KQCQVLS"/>
    <dbReference type="OrthoDB" id="1708389at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016984">
        <property type="term" value="F:ribulose-bisphosphate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019253">
        <property type="term" value="P:reductive pentose-phosphate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03527">
        <property type="entry name" value="RuBisCO_small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00859">
        <property type="entry name" value="RuBisCO_S_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024681">
        <property type="entry name" value="RuBisCO_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000894">
        <property type="entry name" value="RuBisCO_ssu_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036385">
        <property type="entry name" value="RuBisCO_ssu_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31262">
        <property type="entry name" value="PTHR31262"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00101">
        <property type="entry name" value="RuBisCO_small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00961">
        <property type="entry name" value="RuBisCO_small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55239">
        <property type="entry name" value="SSF55239"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0113">Calvin cycle</keyword>
    <keyword evidence="2 3" id="KW-0120">Carbon dioxide fixation</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RuBisCO_small" evidence="4">
        <location>
            <begin position="4"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA06178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="141" mass="16550" checksum="0A9519DF9F9FDF34" modified="2005-10-11" version="1">MRITQGCFSFLPDLTDDQISRQIQYCLSQNWAVNIEFTDDPHPRNTYWEMWNLPMFDLPDAAGVLMELKECRKVYGDRYIRISGFDSSPGWESVKISFIVNRPKNEPGFRLERQESDSRNVRYTTTSYAVAERSEGQRYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNR1</accession>
    <name>Q3SNR1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PIN_12 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2827</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316020.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2827"/>
    <dbReference type="EnsemblBacteria" id="ABA06080">
        <property type="protein sequence ID" value="ABA06080"/>
        <property type="gene ID" value="Nwi_2827"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2827"/>
    <dbReference type="eggNOG" id="ENOG5032VMM">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1155319_0_0_5"/>
    <dbReference type="OMA" id="WIDNIDA"/>
    <dbReference type="OrthoDB" id="1821960at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032557">
        <property type="entry name" value="DUF4935"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16289">
        <property type="entry name" value="PIN_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN_12" evidence="1">
        <location>
            <begin position="20"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="236" mass="25807" checksum="10496DC9EB4A6936" modified="2005-10-11" version="1">MTLQNLDDIVAAQVPLILPDTCILLDILRSPRRDNVDARSVLSAKAIAEAVKLIGSVGSVVASQVCDELSDNRGRVRQETEDGLRKLQIELQRIDGWSGALGVNGQADLSHGLNAVSRCELILVDWVDASLVAPTTDTLTGRAHRRMMQRATPARQGKDSFKDCLVVETYLTLAKELRVKGYTERIVFASSNTAEFIERPGSQLHADIAQEFAVVGIEYARALHEAAYRLGLNTQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNU0</accession>
    <name>Q3SNU0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Succinate dehydrogenase cytochrome b556 subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">Nwi_2798</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Membrane-anchoring subunit of succinate dehydrogenase (SDH).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
        <text evidence="13">The heme is bound between the two transmembrane subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur protein, plus two membrane-anchoring proteins, SdhC and SdhD. The complex can form homotrimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome b560 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2798"/>
    <dbReference type="EnsemblBacteria" id="ABA06051">
        <property type="protein sequence ID" value="ABA06051"/>
        <property type="gene ID" value="Nwi_2798"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2798"/>
    <dbReference type="eggNOG" id="COG2009">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094691_3_1_5"/>
    <dbReference type="OMA" id="GYTWALM"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045281">
        <property type="term" value="C:succinate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000104">
        <property type="term" value="F:succinate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018495">
        <property type="entry name" value="Succ_DH_cyt_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014314">
        <property type="entry name" value="Succ_DH_cytb556"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000701">
        <property type="entry name" value="SuccDH_FuR_B_TM-su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10978">
        <property type="entry name" value="PTHR10978"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01127">
        <property type="entry name" value="Sdh_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000178">
        <property type="entry name" value="SDH_cyt_b560"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81343">
        <property type="entry name" value="SSF81343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02970">
        <property type="entry name" value="succ_dehyd_cytB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01000">
        <property type="entry name" value="SDH_CYT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01001">
        <property type="entry name" value="SDH_CYT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 13" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13" id="KW-0408">Iron</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <note>ligand shared with second transmembrane subunit</note>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000178-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABA06051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="141" mass="15609" checksum="AEE436B7C607E05D" modified="2005-10-11" version="1">MPATSEAMMTVRIERPLSPHLQIYRWTLTMTLSIVHRATGVALYFGTLLLVWWLMAVSSGPGAYAAVQSFTSSWAGRLIAFGYTWALMHHMLGGVRHLVWDLGYGFQPTEREWLTRATLIGGILLTALLWIAAYAIGGGGR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNV5</accession>
    <name>RL19_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplS</name>
        <name type="ordered locus">Nwi_2783</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315981.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SNV5"/>
    <dbReference type="SMR" id="Q3SNV5"/>
    <dbReference type="STRING" id="323098.Nwi_2783"/>
    <dbReference type="EnsemblBacteria" id="ABA06036">
        <property type="protein sequence ID" value="ABA06036"/>
        <property type="gene ID" value="Nwi_2783"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2783"/>
    <dbReference type="eggNOG" id="COG0335">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103507_2_1_5"/>
    <dbReference type="OMA" id="TITVYYE"/>
    <dbReference type="OrthoDB" id="1698718at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00402">
        <property type="entry name" value="Ribosomal_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038657">
        <property type="entry name" value="L19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001857">
        <property type="entry name" value="Ribosomal_L19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018257">
        <property type="entry name" value="Ribosomal_L19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15680">
        <property type="entry name" value="PTHR15680"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01245">
        <property type="entry name" value="Ribosomal_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002191">
        <property type="entry name" value="Ribosomal_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00061">
        <property type="entry name" value="RIBOSOMALL19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01024">
        <property type="entry name" value="rplS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01015">
        <property type="entry name" value="RIBOSOMAL_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000226857" description="50S ribosomal protein L19">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00402"/>
        </source>
    </evidence>
    <sequence length="126" mass="14117" checksum="F772B64BC2A78936" modified="2005-10-11" version="1">MNIIQQLEKEQFDKLSATKTIPEFGPGDTVIVNVKVVEGERTRVQAYEGVCIGRSGGGLNESFTVRKISYGEGVERVFPVMSPMIDSIKVVRRGKVRRAKLYYLRKLRGKSARIVEKKTDRAAATN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNY2</accession>
    <name>Q3SNY2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2756</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315954.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2756"/>
    <dbReference type="EnsemblBacteria" id="ABA06009">
        <property type="protein sequence ID" value="ABA06009"/>
        <property type="gene ID" value="Nwi_2756"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2756"/>
    <dbReference type="eggNOG" id="COG2340">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048111_5_0_5"/>
    <dbReference type="OMA" id="YKVFWTL"/>
    <dbReference type="OrthoDB" id="1705869at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="SSF55797"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP" evidence="1">
        <location>
            <begin position="49"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="169" mass="18024" checksum="4745546AE8072FB7" modified="2005-10-11" version="1">MIRTFAVLLGLLLVAGCVTEKPAAREQPAFYLDMAHGGAKLDSVMAASMISGYRANNGLGPVTVDPELMKIAETQSMAMAKRNKMDHDVAGTLPKRLAAAGYPATLAVENIGAGYHTLAEAFSGWRDSPPHRANMLKNGVTKLGIAASYAPETKYKVFWTLVLASTGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNZ7</accession>
    <name>Q3SNZ7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Efflux pump membrane transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2741</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 4">Cell inner membrane</location>
            <topology evidence="2 4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315939.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2741"/>
    <dbReference type="EnsemblBacteria" id="ABA05994">
        <property type="protein sequence ID" value="ABA05994"/>
        <property type="gene ID" value="Nwi_2741"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2741"/>
    <dbReference type="eggNOG" id="COG0841">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002755_0_1_5"/>
    <dbReference type="OMA" id="LMYMSSS"/>
    <dbReference type="OrthoDB" id="91817at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042908">
        <property type="term" value="P:xenobiotic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2090.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027463">
        <property type="entry name" value="AcrB_DN_DC_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001036">
        <property type="entry name" value="Acrflvin-R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004764">
        <property type="entry name" value="HAE1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32063">
        <property type="entry name" value="PTHR32063"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00873">
        <property type="entry name" value="ACR_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00702">
        <property type="entry name" value="ACRIFLAVINRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82714">
        <property type="entry name" value="SSF82714"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00915">
        <property type="entry name" value="2A0602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="340"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="366"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="434"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="470"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="542"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="868"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="894"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="920"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="969"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1001"/>
            <end position="1023"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1053" mass="112280" checksum="775237732022742E" modified="2005-10-11" version="1">MPAFFIDRPIFAWVVALFICLVGAISIPFLGVAQYPIIAPPSISIATAYPGASPQNLYNSVTRLIEEQLNGASGILNFESTSDSLGQVEITAFFVPGTSTDLASVDVQNRIKRIEARLPAAVLKEGILVEEASSAVLQIITLRSTDDSLDEVGLGDFMTRNIIGEIRRLPGVGRATLFSTERSLRVWLDPEKLVGYGMTSEDVTKAIAAQNAQVASGSIGGPPSRAGQKTAALVLVKGQLGTPEEFGSILLRANRDGSTVRLRDVARVEVGGMDYRFTTRLNGKSTAGLSVLLAPGANALATASAVEAKMEELSKFFPANITYDIPYNITPVVKASIEKVVHTLIEAVVLVFLVMFLFLQNIRYTIIPTIVVPVALLGTCAVLLIAGYSINMLTMFGMVLAIGILVDDAIVVVENVERIMSEEGLPPREAARKAMSQITSAIVGITLVLMAVFVPMAFFPGSVGIIYRQFSVTVVAAIAFSALMALSLTPALCATLLKPVKAGHAHARKGLFGWFNRALEGVRGRYVSTVGWSLRRTGRLMLIYAVLVGVLVWAFVRLPAGFLPVDDQGFITTDVQTPADSSHGRTGAAVEAVERYLAERPGVKNVTFLTGFAYSGQGMNTAQAFITLKDWSERPEKDSAANIVADANRDLASLRDAKITALQPPPIDNLGNSSGFSFRLQDRGQKGYPALLQATNQLIAEANASPILQNVYVEGLPSAGQINLVVDREKASALGVTFEDINDTMSTNLGSTYVNDFPNRGRMQRVIVQSDREGRTTAQDILNYNVKNSSGGLVPLSSFSSIEWSKGPTQVVGFNYYPSMRISGEARAGFTSGDAIAEMERLAGRLPRGFGYEWTGQSLQEKLSGSQAPFLLALSALVVFLCLAALYESWTIPLAVLLTVPLGIFGAVLAATTRGLANDVYFTVGLVTIIGLAAKDAILIVEFAKDLRAQGKSLIDATMEACALRFRPILMTGFAFASGVLPMVIASGAGGASQQALGTSVMGGMIAVVVLALLMVPVFFVVVMRVLGRDKSGLEPAGKAEIHGPPAPMHPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP01</accession>
    <name>Q3SP01_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">Fructose-bisphosphate aldolase</fullName>
            <ecNumber evidence="3 4">4.1.2.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_2737</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:14729"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.1.2.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 5">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the class I fructose-bisphosphate aldolase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.13" evidence="3 4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315935.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2737"/>
    <dbReference type="EnsemblBacteria" id="ABA05990">
        <property type="protein sequence ID" value="ABA05990"/>
        <property type="gene ID" value="Nwi_2737"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2737"/>
    <dbReference type="eggNOG" id="COG3588">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031243_0_0_5"/>
    <dbReference type="OMA" id="DYREMLF"/>
    <dbReference type="OrthoDB" id="945470at2"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00183"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029768">
        <property type="entry name" value="Aldolase_I_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000741">
        <property type="entry name" value="FBA_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11627">
        <property type="entry name" value="PTHR11627"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00274">
        <property type="entry name" value="Glycolytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00158">
        <property type="entry name" value="ALDOLASE_CLASS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="4 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="343" mass="37289" checksum="BC539562A048A45C" modified="2005-10-11" version="1">MNLDDLNKVAQAMVAPGKGILAADESSPTITKRFNAIGVTSTEESRRDYREMMFRATDAMTQYISGVILYDETIWQSARDGTPLIRLIEQAGAIPGIKVDEGTQPLPACSGELVTVGLDRLAERLNKYHAQGARFAKWRAVIDINAADRIPSMTAIGVNAHALGRYAALCQAAQIVPIVEPEVLMDGDHDIDRCLEVTTRVLNVTFQELRKQRVALEGMILKPNMAISGKKSARKAPVEEVAEKTVRMLKACVPAAVPGIAFLSGGQSDEEATAHLDAMNRMGNLPWKLTFSYGRALQAAPQKAWSGKPENVVAGQRALIHRARMNSLATRGAWSADLEEKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP90</accession>
    <name>Q3SP90_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Copper-containing nitrite reductase</fullName>
            <ecNumber evidence="5">1.7.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_2648</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Fe(III)-[cytochrome c] + H2O + nitric oxide = Fe(II)-[cytochrome c] + 2 H(+) + nitrite</text>
            <dbReference type="Rhea" id="RHEA:15233"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16301"/>
            <dbReference type="ChEBI" id="CHEBI:16480"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.7.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu(+)</name>
            <dbReference type="ChEBI" id="CHEBI:49552"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.7.2.1" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2648"/>
    <dbReference type="EnsemblBacteria" id="ABA05901">
        <property type="protein sequence ID" value="ABA05901"/>
        <property type="gene ID" value="Nwi_2648"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2648"/>
    <dbReference type="eggNOG" id="COG2132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031740_1_1_5"/>
    <dbReference type="OMA" id="APCVGCK"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050421">
        <property type="term" value="F:nitrite reductase (NO-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001287">
        <property type="entry name" value="NO2-reductase_Cu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="PTHR11709"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00695">
        <property type="entry name" value="CUNO2RDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="SSF49503"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229083" description="Copper-containing nitrite reductase" evidence="8">
        <location>
            <begin position="39"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="9">
        <location>
            <begin position="57"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA05901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="323" mass="35210" checksum="AE1833FAF0D39FC3" modified="2005-10-11" version="1">MKGRKISMTTVRLRNGVIAGSVAAVMMLAGAAITAAQAKTVKIELVAKEVTVPLDNQGNTVAAWTFNGQVPGPIIRATEGDTIEFTLKNDAANKKSHSIDMHSARSDVLTDFASIKPGETKTFSYVPEHPGVFFYHCGSDPMIQHIARGMFGVVIIDPKDPNAMPKADREYVLIEHQIFENPEDVDSMMANKWKRNAFNAVPFQYDPVHDEHATQTLEAKPGERVRIYMVNAGPNEFSGFHPIAGIWDRIYPSGNPKNVMVGMQNYTLAPGDAATFDLISPKEGAYALVNHSMRAALTGGIAILMISDKADPSMGRGDKILVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPC7</accession>
    <name>Q3SPC7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">LemA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_2611</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LemA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315814.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2611"/>
    <dbReference type="EnsemblBacteria" id="ABA05864">
        <property type="protein sequence ID" value="ABA05864"/>
        <property type="gene ID" value="Nwi_2611"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2611"/>
    <dbReference type="eggNOG" id="COG1704">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056714_0_1_5"/>
    <dbReference type="OMA" id="YNNAVQA"/>
    <dbReference type="OrthoDB" id="1573349at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023353">
        <property type="entry name" value="LemA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007156">
        <property type="entry name" value="MamQ_LemA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34478">
        <property type="entry name" value="PTHR34478"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04011">
        <property type="entry name" value="LemA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140478">
        <property type="entry name" value="SSF140478"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="186" mass="19997" checksum="D760EA360DF0D598" modified="2005-10-11" version="1">MSTGLIIFGVIVAAAVFALIAYNRLVALSQRVGQAFADIDVQLKQRHDLIPNLVETVKGYAAHERGTLDDVIKARNIAMSAQGPAQVGAAENQLSGALGRLIALSEAYPDLKANANFQQLASELSDIENKVAASRRFFNNAVQEYNTAIQQLPAALFAGAFGFTPKEFFDLGASRATVEQTPQVKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPC8</accession>
    <name>Q3SPC8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2610</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05863">
        <property type="protein sequence ID" value="ABA05863"/>
        <property type="gene ID" value="Nwi_2610"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2610"/>
    <dbReference type="eggNOG" id="COG2832">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113299_0_1_5"/>
    <dbReference type="OMA" id="HHWPVFL"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007401">
        <property type="entry name" value="DUF454"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35813">
        <property type="entry name" value="PTHR35813"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04304">
        <property type="entry name" value="DUF454"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016789">
        <property type="entry name" value="DUF454"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="142" mass="15142" checksum="134C13427418A028" modified="2005-10-11" version="1">MIADEPSFGAGAAGALDSRVGTSRMMRGVFLVAGIFFVALGFIGAFLPVLPTTPFLILAAACFTRSSPRLENWLLQHPRFGSLLRAWRERGAIPVKAKLMALGGMAIGFAGFWFGSHPGPWLTAAVALLMLTGLAYVFTRPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPD6</accession>
    <name>Q3SPD6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_2602</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315805.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2602"/>
    <dbReference type="EnsemblBacteria" id="ABA05855">
        <property type="protein sequence ID" value="ABA05855"/>
        <property type="gene ID" value="Nwi_2602"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2602"/>
    <dbReference type="eggNOG" id="COG2321">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059329_0_0_5"/>
    <dbReference type="OMA" id="QKRSQGY"/>
    <dbReference type="OrthoDB" id="1175086at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007343">
        <property type="entry name" value="Uncharacterised_pept_Zn_put"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30168">
        <property type="entry name" value="PTHR30168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04228">
        <property type="entry name" value="Zn_peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="308" mass="33189" checksum="5773EFDAB9735807" modified="2005-10-11" version="1">MRDDDFRRSDNIDDRRGQGGFGGGGFGFPRGRGGLGIGTILILGIVGYAFGIDPRLLIGGAEILTGGGQAPSYQTDGRGSQGKPGAPTDEMGSMISGVLGEIDDRWDEIFKASGQRYSGPRIVLFRNATNGGRCGMAQSAMGPFYCPPDKQIFLDTEFFKQVETRFRGCSGSACRFTAAYIIAHEAGHHIQNLLGILPRVTRLQQQAGSKAEANALQVRVELQADCLSGVWVNRQAKKRPNFLEPGDIEAALTTASAIGDDTLQRQSTGRVVPDSFTHGSAVQRKRWFMTGYQQGTVQACNTFATDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPH0</accession>
    <name>Q3SPH0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">YdjC-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2568</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315772.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2568"/>
    <dbReference type="EnsemblBacteria" id="ABA05821">
        <property type="protein sequence ID" value="ABA05821"/>
        <property type="gene ID" value="Nwi_2568"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2568"/>
    <dbReference type="eggNOG" id="COG3394">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064244_3_0_5"/>
    <dbReference type="OMA" id="FVDGHQH"/>
    <dbReference type="OrthoDB" id="1204930at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006879">
        <property type="entry name" value="YdjC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31609">
        <property type="entry name" value="PTHR31609"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04794">
        <property type="entry name" value="YdjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="291" mass="32138" checksum="DE1D7F05AEA5DFFB" modified="2005-10-11" version="1">MSGDDIILDRADPKPLQRRIRLCADDYGISPGVNRAIRDLIERERINATSVMVVGPAAGRDEVEALKTAASRHPHCAIGLHLTLTAPFHPLTLHYRPLDGGLFPPLSKMLRASLSRRLEPDMIFDEVTAQIAAFGERFGRAPEYVDGHQHVQIFPQVRDAFLNAVKTAAPGAWVRQCGRSRHSMTTLFTTPKALLLDILSAGFRREAARTGIGFNPAFAGAYDFNKPDFAAQMQQFLNGSPDDGLIMCHPGFVDDTLVAFDPLTDQREREYEFLAGDHFADMLAANRVTLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPI5</accession>
    <name>Q3SPI5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Exodeoxyribonuclease 7 large subunit</fullName>
            <ecNumber evidence="5">3.1.11.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Exodeoxyribonuclease VII large subunit</fullName>
            <shortName evidence="5">Exonuclease VII large subunit</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">xseA</name>
        <name evidence="10" type="ordered locus">Nwi_2553</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>Exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterooligomer composed of large and small subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the XseA family.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.6" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315757.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2553"/>
    <dbReference type="EnsemblBacteria" id="ABA05806">
        <property type="protein sequence ID" value="ABA05806"/>
        <property type="gene ID" value="Nwi_2553"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2553"/>
    <dbReference type="eggNOG" id="COG1570">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023625_3_1_5"/>
    <dbReference type="OMA" id="LVWPVKV"/>
    <dbReference type="OrthoDB" id="1371775at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009318">
        <property type="term" value="C:exodeoxyribonuclease VII complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008855">
        <property type="term" value="F:exodeoxyribonuclease VII activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04489">
        <property type="entry name" value="ExoVII_LU_OBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00378">
        <property type="entry name" value="Exonuc_7_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003753">
        <property type="entry name" value="Exonuc_VII_L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020579">
        <property type="entry name" value="Exonuc_VII_lsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025824">
        <property type="entry name" value="OB-fold_nuc-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30008">
        <property type="entry name" value="PTHR30008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02601">
        <property type="entry name" value="Exonuc_VII_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13742">
        <property type="entry name" value="tRNA_anti_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00237">
        <property type="entry name" value="xseA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5 6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5 6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA_anti_2" evidence="9">
        <location>
            <begin position="15"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Exonuc_VII_L" evidence="8">
        <location>
            <begin position="132"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="500"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="500"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA05806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="541" mass="58443" checksum="6A6BAD905A19CF4C" modified="2005-10-11" version="1">MPANPAQLLANAPEYTVSELSSALKRTVENAYGHVRVRGEISGFRGPHASGHCYFALKDESAKIEAVIWKGVHGRMRFKPQEGLEVIATGKLTTYPGSSKYQIVIEAIEPAGIGALMALMEERKKKLAAQGLFDEARKQLLPWLPEVIGVVTSPTGAVIRDILHRLEDRFPRRVLVWPVRVQGEGSAEQIAAAIDGFNALPDGGPIPRPDLLIVARGGGSLEDLWSFNEEIVVRAAAGSMIPLISAVGHETDVTLIDFAADQRAPTPTAAAEMAVPVRSDLAIEISDLAARALVAWKRGHDDRRTELRSAARALPGAGELLAIPRQKLDNAAAALPRALRASTHLHHRRFTRISGGLTLRVLHAQITHAKQSVAATGARLAHCTQVMLRHRRDRFAGLAVRLRASRVANVQAQRDRIARDNERTRRLAERARRALVTLLRRHEARITHSGQLLAALSYRGVLARGFALVRDDAGQAVRSATAAGSGARLTLEFADGKVNVTSDTDQTAATSSNPPRSGPTSEPKPVSRKRAAKPAGQGSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPK8</accession>
    <name>Q3SPK8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315734.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2530"/>
    <dbReference type="EnsemblBacteria" id="ABA05783">
        <property type="protein sequence ID" value="ABA05783"/>
        <property type="gene ID" value="Nwi_2530"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2530"/>
    <dbReference type="eggNOG" id="ENOG5032SR7">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132144_0_0_5"/>
    <dbReference type="OMA" id="WAFNPAC"/>
    <dbReference type="OrthoDB" id="1761037at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229100" evidence="1">
        <location>
            <begin position="33"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="151" mass="16666" checksum="21B6CE7C550BF080" modified="2005-10-11" version="1">MRNLITSLKKCAVGVTLGAALGLGSTMPDAHAFNFPVFGPSYEGQLPRCEAGLPTITTQFWEKERKFWNSPLRITAYGNVHERAFRPRESDNIPRRYCTAEAMVSDGIVRTVHYSIVEDGGIASMGQGVEWCVVGLDRNWAYNPGCLTAQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPN2</accession>
    <name>Q3SPN2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2506</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2506"/>
    <dbReference type="EnsemblBacteria" id="ABA05759">
        <property type="protein sequence ID" value="ABA05759"/>
        <property type="gene ID" value="Nwi_2506"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2506"/>
    <dbReference type="eggNOG" id="ENOG5032I0K">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2718268_0_0_5"/>
    <dbReference type="OMA" id="WTVVKGY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="72" mass="7710" checksum="E9CAC39E164A13B3" modified="2005-10-11" version="1">MLRAERSLEDAISKTLYEAGDAMADRTGLKIVGVLFATVMLAVTLATWTVVKGYADGVYSFESAAVARIAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPP2</accession>
    <name>Q3SPP2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2496</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315702.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2496"/>
    <dbReference type="EnsemblBacteria" id="ABA05749">
        <property type="protein sequence ID" value="ABA05749"/>
        <property type="gene ID" value="Nwi_2496"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2496"/>
    <dbReference type="eggNOG" id="ENOG50301X3">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150046_0_0_5"/>
    <dbReference type="OMA" id="RQMAKHA"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="111" mass="12190" checksum="EA926E6AE4F9DB9C" modified="2005-10-11" version="1">MADADLDVIIRRLAKQQHKSLIAAVKARRDRYLALAAKAKDAAGRQRLRQMAKHAFEEGAAAARRMQMSADNAADSYARAMRRAADRLATEQAATPKKKSVKATKRKAGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPQ5</accession>
    <name>Q3SPQ5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2483</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315689.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2483"/>
    <dbReference type="EnsemblBacteria" id="ABA05736">
        <property type="protein sequence ID" value="ABA05736"/>
        <property type="gene ID" value="Nwi_2483"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2483"/>
    <dbReference type="eggNOG" id="COG2929">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149290_3_0_5"/>
    <dbReference type="OMA" id="ARYMHEK"/>
    <dbReference type="OrthoDB" id="2065431at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038573">
        <property type="entry name" value="BrnT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007460">
        <property type="entry name" value="BrnT_toxin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04365">
        <property type="entry name" value="BrnT_toxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="99" mass="11472" checksum="99B8CC26DC9BD497" modified="2005-10-11" version="1">MIDFDRIAGFEWDDGNRRKNADKHDVSSAEAESIFFNDPLIVAGDEKHSRSEQRFNALGQTTEQRLLHVTFTLRRSGTLIRVISACDMNRKERKIYEQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPW6</accession>
    <name>Q3SPW6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">8-amino-7-oxononanoate synthase</fullName>
            <ecNumber evidence="2">2.3.1.47</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2421</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.47" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2421"/>
    <dbReference type="EnsemblBacteria" id="ABA05675">
        <property type="protein sequence ID" value="ABA05675"/>
        <property type="gene ID" value="Nwi_2421"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2421"/>
    <dbReference type="eggNOG" id="COG0156">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015846_11_2_5"/>
    <dbReference type="OMA" id="YPYFRPI"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008710">
        <property type="term" value="F:8-amino-7-oxononanoate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="1">
        <location>
            <begin position="29"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="378" mass="40434" checksum="280933BA71D5EB2E" modified="2005-10-11" version="1">MTIHGTYRTDLQALADKGRLRTLRGCSGIDFASNDYLGLAESEELRQAAADALARGVPVGSGGSRLLRGNHPEHEALEREAAVFFGAESALYFGGGYVANFAIFSTLPQRGDLVVHDELIHASVHDGVRRGRAEFVSARHNDADAFDAAIVRWRGAGGMGRPWLSIESLYSMDGDSPNLQDLFAVADRHEAMVVIDEAHATGVLGPQGRGLAAPFEGRDNVITLHTCGKALGTSGGFILAPRVIRDFLVNRARPFIYATAPSPLTAAVTRAALELSRTGSARRERLAHLVRSAGGELRRCCHIEPSGSHIVPVIVGADQAAVNLAASLQRRGFDIRAIRPPTVPEGTARLRIALTLHVDEAIVADLFAALAEDMRFAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPY5</accession>
    <name>Q3SPY5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2402</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05656">
        <property type="protein sequence ID" value="ABA05656"/>
        <property type="gene ID" value="Nwi_2402"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2402"/>
    <dbReference type="eggNOG" id="ENOG5032JRU">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_558764_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="386"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="421"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="447"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="488" mass="53627" checksum="85FDBBC5101FDFE6" modified="2005-10-11" version="1">MTFDAWIARRKPLPLPSRTTLNRTVVIASLFGLAGIAMIALDRTVLSGINNSGYAALLRCAPELVDYIEIKRTVLIYLGYVIFSFGYASLALFLLRGEEIQGWPATLAQLSIFSPVGYALLYSGRMPILLVIALIISVGLVRLVQGRSFLPRGHHLFIKTVLFAILFMFYANAMWSARQSFCTDMSGLVEELRSRVNEQASRAVNKANERREEILRQLGSASLDQEKKLRQQLAALEGIIATAGKGGPETSMGAENLSRMIETRKAAVTLPIELRGGRTDFAMFAAVMRDAWHAQPRSYVLSAVESQFIPENSMRSILSNYFYLTHGVRTLDAVWQARENLTPVWGVYEVGILSPLLRVFLPDNQILSLMSSQLRSAEIYGFFPSVWGAAYIDFGASGAMIYIMLWGLVAGLSFRGSRVSTLATPPLILTFIFSSIALSPLQAPLGIANSALVLVSILIVGLAVDFTIIKRGRSTLIAVDARSESNVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPZ2</accession>
    <name>Q3SPZ2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Hexapeptide transferase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2395</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315610.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2395"/>
    <dbReference type="EnsemblBacteria" id="ABA05649">
        <property type="protein sequence ID" value="ABA05649"/>
        <property type="gene ID" value="Nwi_2395"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2395"/>
    <dbReference type="eggNOG" id="COG0110">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081811_2_3_5"/>
    <dbReference type="OMA" id="RIGRWVT"/>
    <dbReference type="OrthoDB" id="1878940at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03360">
        <property type="entry name" value="LbH_AT_putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041561">
        <property type="entry name" value="PglD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020019">
        <property type="entry name" value="Sia_OAcTrfase_NeuD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17836">
        <property type="entry name" value="PglD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03570">
        <property type="entry name" value="NeuD_NnaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PglD_N" evidence="4">
        <location>
            <begin position="3"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="212" mass="21735" checksum="ADC614544F2C6D32" modified="2005-10-11" version="1">MTLVVLCAGGHASVVIEALLSTGTRPDFATDQNPSLVGKLIQGIPIKGPDAAVLEMPVETTELVNAMGNRATRSDSGLSRRRDLFCRFDKLGFRFPVIAHRSAIVASTVQPGDGCQVMAGAVIQPRAQIGRNVLINTRAVVEHDCHVGDHSHIAPGAVLCGGVLVGESVHVGAGAIVLGGVRLGAGSVVAAGATVTRDIEGGCFSGRQDGTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPZ7</accession>
    <name>Q3SPZ7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Imidazole glycerol-phosphate synthase</fullName>
            <ecNumber evidence="5">4.3.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">IGP synthase cyclase subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_2390</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H(+) + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="4.3.2.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterodimer of HisH and HisF.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the HisA/HisF family.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.10" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315605.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2390"/>
    <dbReference type="EnsemblBacteria" id="ABA05644">
        <property type="protein sequence ID" value="ABA05644"/>
        <property type="gene ID" value="Nwi_2390"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2390"/>
    <dbReference type="eggNOG" id="COG0107">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048577_4_0_5"/>
    <dbReference type="OrthoDB" id="1522718at2"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000107">
        <property type="term" value="F:imidazoleglycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016833">
        <property type="term" value="F:oxo-acid-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04731">
        <property type="entry name" value="HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020021">
        <property type="entry name" value="Glycosyl_amidation-assoc_WbuZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004651">
        <property type="entry name" value="HisF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03572">
        <property type="entry name" value="WbuZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA05644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="258" mass="28274" checksum="6B06ABC19672076B" modified="2005-10-11" version="1">MLKKRLIPKFLLRDGRLVKYTCFFDNERIAGNPVTTAKVYNDYGVDELIVLDIVPSAASRARVEDIIGRMSEEIFMPFTVGGGVKTAEDVNALLRAGADKVSINSEAVRRPEFVREAARTFGDQCITVSIDYKRIGSHHASVYIDGGREHVALDPVEWALRCEDLHCGEILMSSIDRDGTMQGYDLEMINTLTKRLGVPMIVSGGCGSLQHAIDALEAGASAVAISSMFLFTDHSPIKLRSHLASRGLDVRTSASSRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ34</accession>
    <name>Q3SQ34_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fumarate hydratase class II</fullName>
            <shortName evidence="2">Fumarase C</shortName>
            <ecNumber evidence="2">4.2.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Aerobic fumarase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Iron-independent fumarase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">fumC</name>
        <name evidence="5" type="ordered locus">Nwi_2353</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(S)-malate = fumarate + H2O</text>
            <dbReference type="Rhea" id="RHEA:12460"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="EC" id="4.2.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="2">There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.2" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315571.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2353"/>
    <dbReference type="EnsemblBacteria" id="ABA05607">
        <property type="protein sequence ID" value="ABA05607"/>
        <property type="gene ID" value="Nwi_2353"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2353"/>
    <dbReference type="eggNOG" id="COG0114">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021594_4_1_5"/>
    <dbReference type="OMA" id="HDSMGEV"/>
    <dbReference type="OrthoDB" id="734949at2"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER01007"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045239">
        <property type="term" value="C:tricarboxylic acid cycle enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004333">
        <property type="term" value="F:fumarate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006106">
        <property type="term" value="P:fumarate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01362">
        <property type="entry name" value="Fumarase_classII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00743">
        <property type="entry name" value="FumaraseC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005677">
        <property type="entry name" value="Fum_hydII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018951">
        <property type="entry name" value="Fumarase_C_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11444">
        <property type="entry name" value="PTHR11444"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10415">
        <property type="entry name" value="FumaraseC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00979">
        <property type="entry name" value="fumC_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lyase_1" evidence="3">
        <location>
            <begin position="18"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="FumaraseC_C" evidence="4">
        <location>
            <begin position="414"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="104"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding (B site)" evidence="2">
        <location>
            <begin position="135"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="145"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="330"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="2">
        <location>
            <position position="194"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="324"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="2">
        <location>
            <position position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="469" mass="50878" checksum="993BB4B71D0FBED5" modified="2005-10-11" version="1">MATSSRTAKTRTETDSFGAIEVQADRYWGAQTERSRQNFRIGEDRMPMPLIRALGIVKLAAAQTNQELGLLDRRRAKAIIRAAREVIEGKLDDHFPLVVWQTGSGTQTNMNLNEVIANRANEMLGGKLGDKKPVHPNDHVNMSQSSNDTFPTAMHIAAAHGIVSDLVPALAALLRALRKKEKAFASIVKIGRTHTQDATPLTLGQEFSGYAAQVESGLTRLRMALNDLYPLAQGGTAVGTGLNTKPKFAKLFARNAALLAKLPFKSAPNKFEALASNDAYVFAHGAINAVATGLFKIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAITMVCCRVFGNHTTMTVAGSQGHFELNVYKPVLAYCMIDSIRLLADATRSFTEHCIKGIRADEQRISELMQRSLMLVTALAPKIGYDNAAKVAKAAHDRGTTLKEEAQRLGFITAAEFDRLVQPSKMTRPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ51</accession>
    <name>Q3SQ51_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Resolvase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2336</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315554.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2336"/>
    <dbReference type="EnsemblBacteria" id="ABA05590">
        <property type="protein sequence ID" value="ABA05590"/>
        <property type="gene ID" value="Nwi_2336"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2336"/>
    <dbReference type="eggNOG" id="COG1961">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010686_18_15_5"/>
    <dbReference type="OrthoDB" id="1791095at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038109">
        <property type="entry name" value="DNA_bind_recomb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011109">
        <property type="entry name" value="DNA_bind_recombinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07508">
        <property type="entry name" value="Recombinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="SSF53041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51737">
        <property type="entry name" value="RECOMBINASE_DNA_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="1">
        <location>
            <begin position="13"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Recombinase" evidence="2">
        <location>
            <begin position="174"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="532" mass="59930" checksum="9B1D72B0B37C3B91" modified="2005-10-11" version="1">MRAGKALSREIVRCAIYTRVSTDNGLDQEFNSLDAQFEASSAYIKSQAHAGWVQVKTRRYDDGGFSGGSTDRPALQSLLEDIRARKIDVIVVYKVDRLTRSLADFAKLVELFDAHGVSFVSVTQQFNTTTSMGRLTLNVLLSFAQFEREVTSERIRDKVAASKKKGIWVGGPLPLGYVMENGKVAVVEEEASRVRHIFGRYLELGSVRALMRDLKDRNIVSRQRLLKTGVTRGGVPFGCGALFHLLRNRFYIGEVNYKGQIYPGEQPPILERDLFERVQTMLTDQRSHQTRTRTSFSHLLTGLMFDDAGHRMAPTQAQKKGRHYSYYVSRPLLFGNAATAAVGSVSRVPAPDIDEQVRKVVLEPRHKSTSPIDNTYASLLIERIEVQQNRLVVQLKQDPDITIEENHSTIVIPWTKPPSKRARSVLRPTSAKLPLRPMKLERRATLVAAIARGRRWLDELITDSAIDTQTIAKRERCSLRHVNMTLSLAFLSPVLTKAAVEGRLPRGIGLEQLRELPLQWDRQFEALGFGPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ54</accession>
    <name>Q3SQ54_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2333</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05587">
        <property type="protein sequence ID" value="ABA05587"/>
        <property type="gene ID" value="Nwi_2333"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2333"/>
    <dbReference type="eggNOG" id="COG2849">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_803709_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="345" mass="38636" checksum="6052A711898B91F7" modified="2005-10-11" version="1">MGLFDFLRRKKPRSVLEALQSSPELQPQKELFEAMDAMCETGVDADELPNGIGEYGLTATNPIPCRTIFGSTAYLGRLRAADGAKVAYQRLGSTQSSVSAHPIDMYEVTHPSGRNLATLYVSPYHKRISAKTPRGFTLAENSFAQAAPVRNVVTEPDGTVRYFSCGVLHREDGPAEIEVYDNYDGPERHRSWYRFGVLHREDGPAVVGGRTTNGSYEWWREGVRHRDDGPAVIDLIHSFEFGDGPIYEWWRNGVQQKVQDGDGILFYFKDGETVLTELPDGCRIVDREDEYRFEDADGNDLGDPPISLNAYIGYLEGRECLNELYEEPQSTFDTFNPAPSAQDGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ78</accession>
    <name>Q3SQ78_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycoside hydrolase 15-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2309</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2309"/>
    <dbReference type="CAZy" id="GH15">
        <property type="family name" value="Glycoside Hydrolase Family 15"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05563">
        <property type="protein sequence ID" value="ABA05563"/>
        <property type="gene ID" value="Nwi_2309"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2309"/>
    <dbReference type="eggNOG" id="COG3387">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010399_2_0_5"/>
    <dbReference type="OMA" id="RMRFSYG"/>
    <dbReference type="OrthoDB" id="318658at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011613">
        <property type="entry name" value="GH15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045582">
        <property type="entry name" value="Trehalase-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19291">
        <property type="entry name" value="DUF5911"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00723">
        <property type="entry name" value="Glyco_hydro_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5911" evidence="2">
        <location>
            <begin position="13"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_hydro_15" evidence="1">
        <location>
            <begin position="229"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="617" mass="68883" checksum="837E972C2C4C0EAF" modified="2005-10-11" version="1">MSIGMFNSVTTRAIGDYALIGDCETAALVAKDGSIDWLCWPRFDSGACFAALLGTEKHGRWRISPARELTRRSRRYRGDTLILETEFETQEGAVSLIDFMPIRKNRQVSEIVRMVVGKRGRVDMRMEVAFRFDYGRIVPWVSRIEGGLLRAVAGPHSAILKSPVATHGENFTTVAEFSVGEGERVPFVLAHEASHLPPPCICDPEQALDETEAYWSEWSGRCHYDGPWKEAVTRSLITVKALTYRPTGGIVAAPTTSLPEQFGSTRNWDYRFCWLRDATFTLLSLINAGYRTEAESWCDWLLRAVAGAASQIQPLYGIGGEHRNDEVELDWLPGFGGSRPVRIGNGAYSQLQVDVFGSVMDALHAATAFGLDLHEASAGLQRELLKHLESVWREPDEGIWEVRSGRKHFVHSKLMSWVAFDRAVISCERFGLAGPVDRWRALRDEIHAEIIEKGFDAEQGAFMQSYGSKAFDASVLLMPILGFLPPDDPRIVSTTRTIERELSKDGLLRRYDTGKAEDGLPASEGAFLACSFWLADNMILQGREQEARRLFEHLLSLRNDVGLLAEQYDVKRQAQAGNFPQAFSHFALIDTAFNFAGIKGAAREVHMKSQETARTGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQA3</accession>
    <name>Q3SQA3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Secretion protein HlyD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2284</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315505.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2284"/>
    <dbReference type="EnsemblBacteria" id="ABA05538">
        <property type="protein sequence ID" value="ABA05538"/>
        <property type="gene ID" value="Nwi_2284"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2284"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018816_1_2_5"/>
    <dbReference type="OMA" id="TTGERWQ"/>
    <dbReference type="OrthoDB" id="1432684at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032317">
        <property type="entry name" value="HlyD_D23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006143">
        <property type="entry name" value="RND_pump_MFP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16576">
        <property type="entry name" value="HlyD_D23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01730">
        <property type="entry name" value="RND_mfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="HlyD_D23" evidence="4">
        <location>
            <begin position="98"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="403" mass="43434" checksum="19B440224FF0EE02" modified="2005-10-11" version="1">MTVRREGDTGDMTEVPPRELNEKPRTRPVRMTRWFIIGGILLSLLVGGLVGFNAFRAHMIAQFFANNKPPPVTISAVEVKSEVLPNLQRAVGSLAAVHQVNVSSDVNGRITEILFTAGSRVVKGAPLVQLFDKPEQGDLASFKAQATVSRLALERAKQLAARQVGPQVTVDQAQAAFDQANAGIAKTEAIISQKLVRAPFDGVLGVRQVEVGQFLNAGTPIVSLTDLSTLFANFTVTEKDSARLAVGQTVRVSVDAYPGREFDGKITTIEPQIATETRNIRVQATIANPDNILKPGMFADVIVVLPDKPPAVTVPETAVDYTLYGDSVYLIKEKKEDDGKTSLTVERTFVRTGDRINGRAIIEKGVKPGDRVAAVGQLKLQSGAAVEISKDQMPQIPAEPPLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQN3</accession>
    <name>Q3SQN3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HI0933-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2154</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315377.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2154"/>
    <dbReference type="EnsemblBacteria" id="ABA05408">
        <property type="protein sequence ID" value="ABA05408"/>
        <property type="gene ID" value="Nwi_2154"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2154"/>
    <dbReference type="eggNOG" id="COG2081">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025174_1_0_5"/>
    <dbReference type="OMA" id="RHRWLGW"/>
    <dbReference type="OrthoDB" id="1513550at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004792">
        <property type="entry name" value="BaiN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022460">
        <property type="entry name" value="Flavoprotein_PP4765"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023166">
        <property type="entry name" value="HI0933-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42887">
        <property type="entry name" value="PTHR42887"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03862">
        <property type="entry name" value="flavo_PP4765"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00275">
        <property type="entry name" value="TIGR00275"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="408" mass="43255" checksum="60F2B263CD8316B0" modified="2005-10-11" version="1">MRDALHTQAAVLGAGPAGLMAAEVLARGGVRVTVFDAMPSAGRKLLMAGRGGLNLTHSEPLPQFLARYGAAATYLQPAIDSFTPAALRQWAGDLDQPTFVGSSGRVFPKAFKASPLLRAWLKRLDTLGVRLALRHRWSGWDHDDRLKFQTPDGERTVAADTTVMALGGASWPRLGSDGAWVEQLASRGIVVSPLRPANCAFDVAWSDVFRDRFEGQPLKNVALSFGPHTVRGEALVTREGIEGGGIYALSAALRERIDIAGNAVLYINLRADAATDQLAARLSTSRGKQSMSNWLRKAAGLSPVAIGLLREAATDHHPASAQPASLAALIHAVPLTLTGIAPLARAISSAGGVAFSEVDDDYMIRRLPGVFAAGEMLDWEAPTGGYLLQASFATGAAAGRGALKWLRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQN5</accession>
    <name>Y2152_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0178 protein Nwi_2152</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">Nwi_2152</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0178 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315375.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SQN5"/>
    <dbReference type="STRING" id="323098.Nwi_2152"/>
    <dbReference type="EnsemblBacteria" id="ABA05406">
        <property type="protein sequence ID" value="ABA05406"/>
        <property type="gene ID" value="Nwi_2152"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2152"/>
    <dbReference type="eggNOG" id="COG1671">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106619_2_1_5"/>
    <dbReference type="OMA" id="CPVKDEI"/>
    <dbReference type="OrthoDB" id="1890924at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00489">
        <property type="entry name" value="UPF0178"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003791">
        <property type="entry name" value="UPF0178"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35146">
        <property type="entry name" value="PTHR35146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02639">
        <property type="entry name" value="DUF188"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000241817" description="UPF0178 protein Nwi_2152">
        <location>
            <begin position="1"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00489"/>
        </source>
    </evidence>
    <sequence length="157" mass="16818" checksum="F366C92CBBE5F330" modified="2005-10-11" version="1">MTAIRLYVDADACPVKDEIYRVAERHGVSVSVVAGGYIRVPDHPLIERIAAGPGMDAADDWIAERAGKTDIVITADIPLASRCVKAGASVIAPNGKPFTEQSIGMTLAVRNLMTDLRASGEMTGGPRSFQPRDRSAFLSALDQAIRRIRRACAARQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQQ3</accession>
    <name>Q3SQQ3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2133</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05388">
        <property type="protein sequence ID" value="ABA05388"/>
        <property type="gene ID" value="Nwi_2133"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2133"/>
    <dbReference type="HOGENOM" id="CLU_2207253_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="107" mass="11859" checksum="287461023EC29815" modified="2005-10-11" version="1">MRPRWRSAGRRRSTPHLLAGRSAAEDGGRETFLFREEWAQPRGRKSTGAIAAQAIEAKPACGQISPSKRPHGALDQAERIKENLAPPSCNREGRRGRRTALIPRHGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQR5</accession>
    <name>Q3SQR5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Antirestriction protein ArdC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2121</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315347.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2121"/>
    <dbReference type="EnsemblBacteria" id="ABA05376">
        <property type="protein sequence ID" value="ABA05376"/>
        <property type="gene ID" value="Nwi_2121"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2121"/>
    <dbReference type="eggNOG" id="COG4227">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041111_0_0_5"/>
    <dbReference type="OMA" id="GFPGQSW"/>
    <dbReference type="OrthoDB" id="1509644at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017113">
        <property type="entry name" value="Antirestriction_ArdC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013610">
        <property type="entry name" value="ArdC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041459">
        <property type="entry name" value="MPTase-PolyVal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08401">
        <property type="entry name" value="ArdcN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18818">
        <property type="entry name" value="MPTase-PolyVal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037112">
        <property type="entry name" value="Antirestriction_ArdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ArdcN" evidence="1">
        <location>
            <begin position="17"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="MPTase-PolyVal" evidence="2">
        <location>
            <begin position="166"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="317" mass="34729" checksum="5AA15BE3FABCDCCD" modified="2005-10-11" version="1">MSRHGRNARAGTDRASLYDDITNRIIAELEAGRVPWVQPWGTAAAKAPLAMPANAATGRQYSGINVLILWGAVVEHGFPVQSWLTFRQALSLGGHVRKGERGTTVVYADRFIPDDEKKRAQETGEEAQAIPFLKRFTVFNVAQCEELPDDLAVAPPAPEPGLIEPRVEALIKATGIDFHIGGERAFYVPAQDYVQVPPPQAYFETINWHRTALHELGHATGHASRLGRDLSGSYGSKKYAFEELVAEMNAAFCCASLGITPTVRHADYIGSWLDVLREDNRAIVRAASQASKAADWLLAFLPETETAVESVNNRRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQR6</accession>
    <name>Q3SQR6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Integrase, catalytic region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1572</name>
        <name evidence="3" type="ordered locus">Nwi_2120</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="3">Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1572"/>
    <dbReference type="EnsemblBacteria" id="ABA04833">
        <property type="protein sequence ID" value="ABA04833"/>
        <property type="gene ID" value="Nwi_1572"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05375">
        <property type="protein sequence ID" value="ABA05375"/>
        <property type="gene ID" value="Nwi_2120"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1572"/>
    <dbReference type="KEGG" id="nwi:Nwi_2120"/>
    <dbReference type="eggNOG" id="COG2801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027402_4_0_5"/>
    <dbReference type="OMA" id="QIAPSGY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025948">
        <property type="entry name" value="HTH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13276">
        <property type="entry name" value="HTH_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="126"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="301" mass="34112" checksum="B259A55771B4961A" modified="2005-10-11" version="1">MIAFIDGHREVHGVEPICRVLPIAPSTYHDDLAKRADPTKLSARAKRDLRLKPEIERVFVENFEVYGVRKVWRQLNRENIPVARCTVERLMADLGLQGVIRGKPIRTTVQDKAAPCPLDHVNRVFHAPAPNMLWLSDFTYVSTWSGFVYVAFVIDAYARRIVGWRVSRAAHAAFVLDALEQALHERRPASKAGLVHHSDRGSQYVSIRYTERLAEAGIEPSVGSVGDSYDNALAETINGLYKAEVIHRRGPWRSFEAVEFATLAWVDWFNHRRLLEPIGNIPPAEAEERYYAMLDETAMAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQX0</accession>
    <name>Q3SQX0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315300.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05321">
        <property type="protein sequence ID" value="ABA05321"/>
        <property type="gene ID" value="Nwi_2063"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2063"/>
    <dbReference type="eggNOG" id="ENOG5030VKY">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132502_0_0_5"/>
    <dbReference type="OMA" id="DPTRYYY"/>
    <dbReference type="OrthoDB" id="1926140at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229136" evidence="1">
        <location>
            <begin position="25"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="186" mass="20522" checksum="5E5C673812AFF104" modified="2005-10-11" version="1">MRQMVSGLVAAAAMVAASVAPAMACGGWYTGGCASCLSYGSCAWGYGPGYYGAGFASYEHLPSPTQYYYVNQGPAFSGPGNFAPVPTYQESAVAGWNAYSRPYYYPYDGGRYAHAMHHYYDGAPAAGPVIYSYRWNRNRQGVHPHSRRHLRYGHASRDGHRAALPRVIYGSDHHHGYRHAVQYRHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR11</accession>
    <name>Q3SR11_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2021</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315266.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2021"/>
    <dbReference type="EnsemblBacteria" id="ABA05280">
        <property type="protein sequence ID" value="ABA05280"/>
        <property type="gene ID" value="Nwi_2021"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2021"/>
    <dbReference type="eggNOG" id="ENOG502ZUHH">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1465558_0_0_5"/>
    <dbReference type="OMA" id="PTDMFDF"/>
    <dbReference type="OrthoDB" id="1916139at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="172" mass="18052" checksum="E22829AAECB83930" modified="2005-10-11" version="1">MRRSERLLTAAVLISLCGMLAGCGGGGLNNWDPTDMFNFLDTKKKMPGNRQPVFPDGVPGIEQGVPKDLYKSDAGLQQEQAVAAPAPPDESEQARRQGAAEGSSRRAAAADSRTAAAPRKPKRLVHRRATSLPKEEAPPAQQAAPPPPAAQQSEPAAAPPFPAPLPSGSFSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="126" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR15</accession>
    <name>Q3SR15_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Amidophosphoribosyltransferase</fullName>
            <shortName evidence="5">ATase</shortName>
            <ecNumber evidence="5">2.4.2.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Glutamine phosphoribosylpyrophosphate amidotransferase</fullName>
            <shortName evidence="5">GPATase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">purF</name>
        <name evidence="11" type="ordered locus">Nwi_2017</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = 5-phospho-alpha-D-ribose 1-diphosphate + H2O + L-glutamine</text>
            <dbReference type="Rhea" id="RHEA:14905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="EC" id="2.4.2.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="5 8">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 5 6">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5 6">In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.14" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315262.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2017"/>
    <dbReference type="MEROPS" id="C44.001"/>
    <dbReference type="EnsemblBacteria" id="ABA05276">
        <property type="protein sequence ID" value="ABA05276"/>
        <property type="gene ID" value="Nwi_2017"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2017"/>
    <dbReference type="eggNOG" id="COG0034">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022389_3_1_5"/>
    <dbReference type="OMA" id="ENAQPTF"/>
    <dbReference type="OrthoDB" id="267682at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00124"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004044">
        <property type="term" value="F:amidophosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00715">
        <property type="entry name" value="GPATase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01931">
        <property type="entry name" value="PurF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005854">
        <property type="entry name" value="PurF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035584">
        <property type="entry name" value="PurF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000485">
        <property type="entry name" value="Amd_phspho_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01134">
        <property type="entry name" value="purF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="5 6 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5 6" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="10">
        <location>
            <begin position="31"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5 7">
        <location>
            <position position="31"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5 8">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 8">
        <location>
            <position position="379"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 8">
        <location>
            <position position="380"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA05276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="504" mass="55103" checksum="D633B7E5F0D7A790" modified="2005-10-11" version="1">MDCQQSLSSDASPNDPDTDFDLDGDTLREECGVFGIFGHPEAAAITALGLHALQHRGQEAAGIVTFDGHRFHSERRLGLVGDAFSRREVIERLPGSAAVGHTRYSTTGGTILRNVQPLFAELHAGGFAVAHNGNLTNGLTLRRELVGRGALMQSTSDTEAILHLVAQSRRGRFIERYIEALRAIEGAYALVSLTNKKLVGARDPRGIRPLVLGELDGCPILASETCALDIIGAKYVRDIEPGEVIVFHRKDDVIQTEIHKPFPPMPPRPCIFEYIYFSRPDSIVAGRSVYEVRKAFGAQLARESHVDVDVVVPVPDSGVPAALGYSRFSGVPYELGIIRNHYVGRTFIQPAQSVRELGVRMKHSANRAAIAGKRIILIDDSLVRGTTSRKIVRMMRDAGATEVHFRLASPPIIHPDYYGIDLPDRNGLLAATHSLEEMREMIGADSLAFLSIDGMYRAMGEPGRDPAHPKYADHCFTGAYPTSLTDHTDVEPQPHQLSLLAEAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR80</accession>
    <name>Q3SR80_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Resolvase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1951</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315202.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1951"/>
    <dbReference type="EnsemblBacteria" id="ABA05211">
        <property type="protein sequence ID" value="ABA05211"/>
        <property type="gene ID" value="Nwi_1951"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1951"/>
    <dbReference type="eggNOG" id="COG1961">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010686_18_15_5"/>
    <dbReference type="OMA" id="NQRWAFQ"/>
    <dbReference type="OrthoDB" id="1791095at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038109">
        <property type="entry name" value="DNA_bind_recomb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011109">
        <property type="entry name" value="DNA_bind_recombinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07508">
        <property type="entry name" value="Recombinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="SSF53041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51737">
        <property type="entry name" value="RECOMBINASE_DNA_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="1">
        <location>
            <begin position="16"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Recombinase" evidence="2">
        <location>
            <begin position="176"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="445" mass="49764" checksum="7C1360C1626EC13F" modified="2005-10-11" version="1">MIRHQEAVAVMSRRQRCAIYTRKSSEEGLDMEFNSLDAQREACESFVTSQKAEGWATIRERYDDGGFSGGTLERPGLKRLIQDVEAGLIDVIVVYKIDRLSRSLMDFARLVEIFDRSQVTFVSVTQSFNTTTSMGRLTLNILLSFAQFEREVIGERIRDKVAASRKRGMWMGGHVPLGYDVRDRKLVVNEAEAATVRMIFERFVAIGSATTLAKALAAEGVLNKRGKQIDKGFLYKLINNRLYLGEAVHKGTAYPGEHEAILDQALWDKVHGILQESPRRRAKNTRRQAPALLKGIIFTDTGTAMTPTATKKGTRLYRYYASMDLIRNRPTGDASGPLRLPAGMVEDAVVGEIRRMIRAPEIAARTIKALREESSIVDEKAVVKALGEFDQLWAALYPAEQTRIVQLLVERVTVGEDGIAVDLRHEGLGSVLRDMMAPRRTEACA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRC0</accession>
    <name>BPT_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartate/glutamate leucyltransferase</fullName>
            <ecNumber evidence="1">2.3.2.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">bpt</name>
        <name type="ordered locus">Nwi_1911</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Functions in the N-end rule pathway of protein degradation where it conjugates Leu from its aminoacyl-tRNA to the N-termini of proteins containing an N-terminal aspartate or glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-leucyl-tRNA(Leu) + N-terminal L-glutamyl-[protein] = H(+) + N-terminal L-leucyl-L-glutamyl-[protein] + tRNA(Leu)</text>
            <dbReference type="Rhea" id="RHEA:50412"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="Rhea" id="RHEA-COMP:12664"/>
            <dbReference type="Rhea" id="RHEA-COMP:12668"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:64721"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:133041"/>
            <dbReference type="EC" id="2.3.2.29"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-leucyl-tRNA(Leu) + N-terminal L-aspartyl-[protein] = H(+) + N-terminal L-leucyl-L-aspartyl-[protein] + tRNA(Leu)</text>
            <dbReference type="Rhea" id="RHEA:50420"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="Rhea" id="RHEA-COMP:12669"/>
            <dbReference type="Rhea" id="RHEA-COMP:12674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:64720"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:133042"/>
            <dbReference type="EC" id="2.3.2.29"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the R-transferase family. Bpt subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.29" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315167.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRC0"/>
    <dbReference type="STRING" id="323098.Nwi_1911"/>
    <dbReference type="PRIDE" id="Q3SRC0"/>
    <dbReference type="EnsemblBacteria" id="ABA05171">
        <property type="protein sequence ID" value="ABA05171"/>
        <property type="gene ID" value="Nwi_1911"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1911"/>
    <dbReference type="eggNOG" id="COG2935">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077607_1_0_5"/>
    <dbReference type="OMA" id="MVEDSHV"/>
    <dbReference type="OrthoDB" id="1675133at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004057">
        <property type="term" value="F:arginyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008914">
        <property type="term" value="F:leucyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071596">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00689">
        <property type="entry name" value="Bpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017138">
        <property type="entry name" value="Asp_Glu_LeuTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030700">
        <property type="entry name" value="N-end_Aminoacyl_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007472">
        <property type="entry name" value="N-end_Aminoacyl_Trfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007471">
        <property type="entry name" value="N-end_Aminoacyl_Trfase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21367">
        <property type="entry name" value="PTHR21367"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04377">
        <property type="entry name" value="ATE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04376">
        <property type="entry name" value="ATE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037208">
        <property type="entry name" value="ATE_pro_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000263195" description="Aspartate/glutamate leucyltransferase">
        <location>
            <begin position="1"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00689"/>
        </source>
    </evidence>
    <sequence length="257" mass="29306" checksum="B0CBD813A6383399" modified="2005-10-11" version="1">MTQHSRDTPQFYLTAPSPCPYLPDRSERKVFTHLVGEKAGELNDLLTHGGFRRSQSIAYRPACDQCRACVSVRVIADEFQLSRGARRVLARNADITGSLRAATPTSEQYSIFRAYLDQRHRHGGMADMTVLDYAMMVEDSHVETRIIEYRRRGADRVDNKRGDLVGVALTDVLSDGLSMVYSFFDPSEQNRSFGTFMILDHISRARQLGLPYVYLGYWIEGSRKMDYKGRFLPQQRLAPTGWLRVDADGETKHETTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRE7</accession>
    <name>Q3SRE7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase FkbM</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1884</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315140.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1884"/>
    <dbReference type="EnsemblBacteria" id="ABA05144">
        <property type="protein sequence ID" value="ABA05144"/>
        <property type="gene ID" value="Nwi_1884"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1884"/>
    <dbReference type="eggNOG" id="COG4122">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_860187_0_0_5"/>
    <dbReference type="OMA" id="HTVSRYI"/>
    <dbReference type="OrthoDB" id="1340956at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006342">
        <property type="entry name" value="FkbM_mtfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05050">
        <property type="entry name" value="Methyltransf_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01444">
        <property type="entry name" value="fkbM_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_21" evidence="1">
        <location>
            <begin position="110"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="321" mass="35174" checksum="49AC4FCCC16B61F5" modified="2005-10-11" version="1">MIAQPPIQFDRTSGVLEGANAWERAASLALVAGSKVSSLFAHRGYNWCANLLRKTLPERDIAVRLNPDATFAFPYGDGYWSKLLNRSFRYEEELELLFRASADVDYTLLDCGANYGYWSVLVTSRPFGSRKAIAIEPSSKNFARLAHNAVINGNRFEVMQSAIGATRGVARLSGTKHEAFSIAGAAGAGGESVPVLSLDDLLDDGRISPNGKHLIKLDVEGVEIEAIKGGARVLQGDAVILCEEHGNDRNHTVSRYILEQTPLTLIVHDPASGRFETLTELSILDRIKVASHVGYNVFGTSSAFWQDRFHRMNATASQRVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRG3</accession>
    <name>Q3SRG3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">TonB-dependent receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_1868</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the TonB-dependent receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315124.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1868"/>
    <dbReference type="EnsemblBacteria" id="ABA05128">
        <property type="protein sequence ID" value="ABA05128"/>
        <property type="gene ID" value="Nwi_1868"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1868"/>
    <dbReference type="eggNOG" id="COG4772">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008287_13_1_5"/>
    <dbReference type="OMA" id="DWDYAPR"/>
    <dbReference type="OrthoDB" id="514956at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012910">
        <property type="entry name" value="Plug_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037066">
        <property type="entry name" value="Plug_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039423">
        <property type="entry name" value="TonB-dep_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000531">
        <property type="entry name" value="TonB-dep_rcpt_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036942">
        <property type="entry name" value="TonB_rcpt_b-brl_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32552">
        <property type="entry name" value="PTHR32552"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07715">
        <property type="entry name" value="Plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00593">
        <property type="entry name" value="TonB_dep_Rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0798">TonB box</keyword>
    <feature type="domain" description="Plug" evidence="7">
        <location>
            <begin position="100"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="TonB_dep_Rec" evidence="6">
        <location>
            <begin position="227"/>
            <end position="733"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA05128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="734" mass="80388" checksum="67D50E4284060AF0" modified="2005-10-11" version="1">MFNHLTRGGGSALYGALAATVAVLLPYDVFAQSVPGTALPPVMITAPTPHRLQSRQAVRSAPRPVPVARQARVPSQAAAPAAVPGSLTVATAQQALQEIQQTPGGVALVTADAYRNSTVSNTIKDVLDYVPGVFAQPKWGDDTRLSIRGSGLSRNFHLRGVQLYMDGIPISTADGYGDFQEIDPTAYKYVEVFKGGNALRLGANSLGGAINFVTPSGRDPFINGVSVDAGAFGFRRLQANTGGSNGPWDGFVTASTQRSDGFRDHSSGQSTRLSGNIGYQFSPDFETRFYLNANHVRQRIPGAVSKFSALTSPQTAYVNNVAGDWQRNIDTVRIGNKTTMRFDDTTVEFGLFGTDRHLMHPIFQWLDYHYKDYGAFVRAVDDRVIGGYRNRLVAGVNVLNGRIDNQQFANLAGQKGALLSSSIDRSRNTSVYIEDSFYFLPNVAAIAGTQFLYATRDRQDRFLSDGDQSGRTEFSLWSPKGGLLWQIDPTWQAFANVSRSAEVPSYGESANGPGIPNIPFTSIRPQRATTYEIGTRARRPDATWELTYYRAQIENELLCLFSAFGNCNVTNADRTMHQGIEAGAGAAIFRNLFERGDRPDRLWLNVAYTFNDFRFVNDPVFGNNLLPGAPRHYVRGELLYKHPNGFYAGPNIEWVPQGYYADSANTLSTDAYAIYGMKAGFDDGGRYSGYIEARNIGNKAYIASASILDRANANSPLFEPGFGRAVYAGFKVRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRH7</accession>
    <name>Q3SRH7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphatidate cytidylyltransferase</fullName>
            <ecNumber evidence="7">2.7.7.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_1854</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 1,2-diacyl-sn-glycero-3-phosphate + CTP + H(+) = a CDP-1,2-diacyl-sn-glycerol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:16229"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="2.7.7.41"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the CDS family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.41" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1854"/>
    <dbReference type="EnsemblBacteria" id="ABA05114">
        <property type="protein sequence ID" value="ABA05114"/>
        <property type="gene ID" value="Nwi_1854"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1854"/>
    <dbReference type="eggNOG" id="COG0575">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037294_1_1_5"/>
    <dbReference type="OMA" id="PKFWPRV"/>
    <dbReference type="UniPathway" id="UPA00557">
        <property type="reaction ID" value="UER00614"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004605">
        <property type="term" value="F:phosphatidate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016024">
        <property type="term" value="P:CDP-diacylglycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000374">
        <property type="entry name" value="PC_trans"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01315">
        <property type="entry name" value="CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="74"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="280" mass="28729" checksum="F6DBFFFE53374378" modified="2005-10-11" version="1">MDQDQRAPAAASGSQNLRTRIMAALVLAPAAIAIAWTGGPLWIALVMLAGIGMYVEWLTIVGAAQEKQTVAAGIAALVVSGLCLAFGYGALSLAALLTGAVAVAAVSRRLRLWSVAGFCYAAAAAFASAQVRLDGDYGFVALMFVLFVVWAADIGGYFAGRGIGGPKLWPRVSPKKTWAGAVGGFAGGLAVAAGFAAFDLGRLAPLMLLAALLSIVSQSGDLFESAVKRRFGVKDSSQIIPGHGGLLDRLDGFVAAIVFAALFGFLRGDADGIGHALMVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRJ1</accession>
    <name>Q3SRJ1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Molybdopterin molybdenumtransferase</fullName>
            <ecNumber evidence="7">2.10.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_1840</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 7">Catalyzes the insertion of molybdate into adenylated molybdopterin with the concomitant release of AMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenylyl-molybdopterin + H(+) + molybdate = AMP + H2O + Mo-molybdopterin</text>
            <dbReference type="Rhea" id="RHEA:35047"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36264"/>
            <dbReference type="ChEBI" id="CHEBI:62727"/>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.10.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 7">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 7">Belongs to the MoeA family.</text>
    </comment>
    <dbReference type="EC" id="2.10.1.1" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315096.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1840"/>
    <dbReference type="EnsemblBacteria" id="ABA05100">
        <property type="protein sequence ID" value="ABA05100"/>
        <property type="gene ID" value="Nwi_1840"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1840"/>
    <dbReference type="eggNOG" id="COG0303">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010186_7_0_5"/>
    <dbReference type="OMA" id="MTGAMVP"/>
    <dbReference type="OrthoDB" id="651103at2"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061599">
        <property type="term" value="F:molybdopterin molybdotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032324">
        <property type="term" value="P:molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00887">
        <property type="entry name" value="MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038987">
        <property type="entry name" value="MoeA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005111">
        <property type="entry name" value="MoeA_C_domain_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036688">
        <property type="entry name" value="MoeA_C_domain_IV_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005110">
        <property type="entry name" value="MoeA_linker/N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036135">
        <property type="entry name" value="MoeA_linker/N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192">
        <property type="entry name" value="PTHR10192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03454">
        <property type="entry name" value="MoeA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03453">
        <property type="entry name" value="MoeA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="SSF53218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63867">
        <property type="entry name" value="SSF63867"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63882">
        <property type="entry name" value="SSF63882"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="6 7" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="300"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="MoCF_biosynth" evidence="9">
        <location>
            <begin position="177"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA05100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="404" mass="42486" checksum="6CD9EDC48EE1982E" modified="2005-10-11" version="1">MALMAVADALAAVLDGVEPLPEETAALEAAHRRVLARDLPSRRTQPPVAMSAMDGYAVRAADIATIPSRLRVIGESAAGRPLDRALRAGEALRIFTGGVVPDGADTVVIQEDTDRDGDHVIVKEATASGKNIRPAGVDFREGDVLIRARTRLTDRHLSLAAGMNYPDLPVHRRPTIAMLATGDELVLPGATPGPGQIILSNGYALRALARGEGAEAIDLGLAADTMDATTAAIRRARDSGADILVTTGGASVGDHDFIKRALEAEGVEMTFWRIAMRPGKPMMYGRLGAMRVIGLPGNPVSAYICAFLFMVPLIRALSGRVDVHHRVEPALLGGDVAANQARQDYLRARLTYSTDGLPIATPVTHQDSSLMGNLAAADALVVRAPFAPAASKGAPCTVIKLSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRP3</accession>
    <name>Q3SRP3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative beta (1-6) glucans synthase</fullName>
            <ecNumber evidence="2">3.2.1.58</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1787</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.2.1.58" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1787"/>
    <dbReference type="CAZy" id="GH17">
        <property type="family name" value="Glycoside Hydrolase Family 17"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05048">
        <property type="protein sequence ID" value="ABA05048"/>
        <property type="gene ID" value="Nwi_1787"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1787"/>
    <dbReference type="eggNOG" id="COG5309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016454_1_0_5"/>
    <dbReference type="OMA" id="QCASYTP"/>
    <dbReference type="OrthoDB" id="926333at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004338">
        <property type="term" value="F:glucan exo-1,3-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="314"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="346"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="420"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="452"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="481"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="505"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="532" mass="58022" checksum="CB15392DA1C41276" modified="2005-10-11" version="1">MALLIISLGLIAAAWWWLGAPIRLARAPIDPAAKLECVSYAPFRGQQTPLVATTEIAPEQIAEDLAQLAKISNCVRTYSIENGLDRLPALAEKVGLKVIQGIWLGNNRLKNLAQINVAIGLAKEYPNVITALVVGNEVLLRGEMTASDLAANIRLVKSQVKVPVTYADVWEYWLRSREVYDAVDFVTIHILPYWEDIPIRSRNAAAHVGAIRKQVAASFPAKEILIGETGWPSAGRMREGALPSRANQARVVSEILDMAKREKFRVNLIEAYDQPWKRQLEGTVGGNWGLIGVDPRELKYPAGEPVRNFPLWKIQMGAGMGLCVLVFGVAMLTLRRKPWKPRWMSWIAVAVSATTAGILLGVAADKLCYESFGLGNSLRWGGLLAAGVIAPLLGANALMSGRALPTFVELIGPRPGRTGSVLTVMLGITLLVTTLIAAETALGFVFDPRYRDFPFAALSMAVAPCWALAWLNRPLTGVRPIAESVFAGLLIIAAVYIGFNEGPKNWQSLWTCVVYAMLGITLWRARAAQTQG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRV8</accession>
    <name>NUSB_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription antitermination protein NusB</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Antitermination factor NusB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nusB</name>
        <name type="ordered locus">Nwi_1722</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in transcription antitermination. Required for transcription of ribosomal RNA (rRNA) genes. Binds specifically to the boxA antiterminator sequence of the ribosomal RNA (rrn) operons.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NusB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314979.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRV8"/>
    <dbReference type="SMR" id="Q3SRV8"/>
    <dbReference type="STRING" id="323098.Nwi_1722"/>
    <dbReference type="EnsemblBacteria" id="ABA04983">
        <property type="protein sequence ID" value="ABA04983"/>
        <property type="gene ID" value="Nwi_1722"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1722"/>
    <dbReference type="eggNOG" id="COG0781">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087843_4_0_5"/>
    <dbReference type="OMA" id="LYQMDIA"/>
    <dbReference type="OrthoDB" id="1574817at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00073">
        <property type="entry name" value="NusB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035926">
        <property type="entry name" value="NusB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011605">
        <property type="entry name" value="NusB_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006027">
        <property type="entry name" value="NusB_RsmB_TIM44"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11078">
        <property type="entry name" value="PTHR11078"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01029">
        <property type="entry name" value="NusB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48013">
        <property type="entry name" value="SSF48013"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01951">
        <property type="entry name" value="nusB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0889">Transcription antitermination</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000265551" description="Transcription antitermination protein NusB">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00073"/>
        </source>
    </evidence>
    <sequence length="161" mass="17824" checksum="BF197709B4890842" modified="2005-10-11" version="1">MADIKASIDRKANRRGAARLAAVQALYQMEIGGAGINDVFAEFDSHWLGNEVEGDRYLPAEAAFFRDVVAGVVRDQVRIDPLIDDALSKSWPLKRVAAILRAVLRAGSYELEHRKDVPARVVVSEYVDVAHAFVEKDEAGMVNAVLDQIARRFRTGEFARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRW4</accession>
    <name>Q3SRW4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Penicillin-binding protein 1A</fullName>
            <ecNumber evidence="7">2.4.1.129</ecNumber>
            <ecNumber evidence="6">3.4.16.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ordered locus">Nwi_1716</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>[GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = [GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23708"/>
            <dbReference type="Rhea" id="RHEA-COMP:9602"/>
            <dbReference type="Rhea" id="RHEA-COMP:9603"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:60033"/>
            <dbReference type="ChEBI" id="CHEBI:78435"/>
            <dbReference type="EC" id="2.4.1.129"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the transpeptidase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the N-terminal section; belongs to the glycosyltransferase 51 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.129" evidence="7"/>
    <dbReference type="EC" id="3.4.16.4" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="SMR" id="Q3SRW4"/>
    <dbReference type="STRING" id="323098.Nwi_1716"/>
    <dbReference type="CAZy" id="GT51">
        <property type="family name" value="Glycosyltransferase Family 51"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04977">
        <property type="protein sequence ID" value="ABA04977"/>
        <property type="gene ID" value="Nwi_1716"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1716"/>
    <dbReference type="eggNOG" id="COG5009">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006354_2_4_5"/>
    <dbReference type="OMA" id="YQLTSMM"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008658">
        <property type="term" value="F:penicillin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008955">
        <property type="term" value="F:peptidoglycan glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009002">
        <property type="term" value="F:serine-type D-Ala-D-Ala carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3810.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001264">
        <property type="entry name" value="Glyco_trans_51"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036950">
        <property type="entry name" value="PBP_transglycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031376">
        <property type="entry name" value="PCB_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001460">
        <property type="entry name" value="PCN-bd_Tpept"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17092">
        <property type="entry name" value="PCB_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00912">
        <property type="entry name" value="Transgly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00905">
        <property type="entry name" value="Transpeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0046">Antibiotic resistance</keyword>
    <keyword evidence="9" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="11 21" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="10" id="KW-0645">Protease</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="21" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="Transgly" evidence="19">
        <location>
            <begin position="60"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="PCB_OB" evidence="20">
        <location>
            <begin position="326"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="Transpeptidase" evidence="18">
        <location>
            <begin position="451"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="806"/>
            <end position="831"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00018638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF17092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ABA04977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="831" mass="91436" checksum="95DF06C0102422E9" modified="2005-10-11" version="1">MMRLLLRFMGFLFAAGTILFLVGVAAVAGLIWHFSQDLPEYSQLQNYEPPVMTRVHASDGSLLGEYAKERRLYLPIQAVPKLVINAFLAAEDKNFYEHGGIDYQGMARAALLYAQNYGTNKRPQGASTITQQVAKNFLLTNEVSFTRKIKEALLAMRLERTYSKDKILELYLNEIYLGLGAYGIAAASLVYFDKSVNELSVAEAAYLAALPKAPSTLHPVKNRERAIERRDYVVDRLLENGWVNQADAEKARKQPLIVTGRSNGAHTFAGEYFAEEVRRDLFERYGEEKLYGGGLSVRTTLDPELQVIARKTMANGLVTYDEAQGWRGPVSKLDISGDWGVKLAEVKSLSDISPWRMAVVLESDDKSARIGFQPGRELGGAVSRERRTGLITLDGVKWAKATSGSARGRTPTKVSQVLSPGDVIYADPLIAKDGKIVEGEYRLRQLPEVSGAMVAMDPRTGRVLAMVGGFSFDQSQFNRATQAYRQPGSSFKPLVYSSALDNGYTPSTVVVDAPIEIDQGRGSGVWRPENYSKEKYYGPQTLRNALRLSLNTVTVRLAQDVGMPLIGEYAKRFGVYDELPNYLSYALGAGETTVLRMVTAYSMFDNGGRRVKPTLIDRIQDRYGHTIYKHDARECRGCEAPEGWKNQSEPQLIDHREQVLDSMTAYQITSMMEGVVQAGTATVMREVGKPIAGKTGTTNDQKDAWFIGFSPDLVVGIYIGYDKPRNLGRRATGGHLAAPIARDFMKIALADKPATPFRIPPGIKLVRVDAKSGMRAGPGSGGKTILEAFKPGTAPPDNYSVIGVADADGREPQPLPDADRSLFRPGTGGLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRX3</accession>
    <name>Q3SRX3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1707</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314966.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1707"/>
    <dbReference type="EnsemblBacteria" id="ABA04968">
        <property type="protein sequence ID" value="ABA04968"/>
        <property type="gene ID" value="Nwi_1707"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1707"/>
    <dbReference type="eggNOG" id="ENOG5032YT1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136716_3_0_5"/>
    <dbReference type="OMA" id="DWHGLLM"/>
    <dbReference type="OrthoDB" id="1872036at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021660">
        <property type="entry name" value="DUF3253"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11625">
        <property type="entry name" value="DUF3253"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="89" mass="9874" checksum="F37CB3948DA174F9" modified="2005-10-11" version="1">MMTQDSHSIPSLENDILKVLTRDATRTLSAPELAQAIAAEADWRSLLMPIRRAAVAMAQSGRLVIYRKGKPADPLDFRGVYRVGLPRLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS51</accession>
    <name>Q3SS51_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RecA-family ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1629</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314889.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1629"/>
    <dbReference type="EnsemblBacteria" id="ABA04890">
        <property type="protein sequence ID" value="ABA04890"/>
        <property type="gene ID" value="Nwi_1629"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1629"/>
    <dbReference type="eggNOG" id="COG3598">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5545">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_412090_0_0_5"/>
    <dbReference type="OMA" id="RINIRCI"/>
    <dbReference type="OrthoDB" id="1706570at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016817">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015330">
        <property type="entry name" value="DNA_primase/pol_bifunc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014819">
        <property type="entry name" value="PriCT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08707">
        <property type="entry name" value="PriCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09250">
        <property type="entry name" value="Prim-Pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00943">
        <property type="entry name" value="Prim-Pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prim-Pol" evidence="2">
        <location>
            <begin position="15"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="336"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="666" mass="72571" checksum="73951060866A3D4B" modified="2005-10-11" version="1">MNNSLYPQAADCASLQYYKSNGAALVPIPAGSKIATGIISSFKHDWSRNPEQIDKWARENPGCNFGVVAFASNLIIVDIDTKGDNGREEAWALWAELCASWGLPAALVPHVQSPSGGWHVYCTVPDGVDAATLRQPDAIKGRINIRCIGYTVAAGSYYDGRPYQLMPGAPPPHPAPEGLIQHCSGGTRKVTEHRSRQGEHSLRDVRSMLAWMNERDAFDSYEDWISIGQALKICFWDDGLELWESVTVAGRYSCDQKWETFKDTDDGNAVTLATWFQRARKAGWQLAASEMFGSVASIGSPSLAPTTPRAALLKSSAEFLEGFVPPDYILDGVLQRGFCYSMTAKTGTGKTAVAMCFSAHVATGRSLNGLSVSQGDVIYLAGENPTDIQMRWLGLTQEMRIDPQTVPVYFLPGVISVSQNVAAIEQEVKERALQPMLVVVDTAAAYFEGDNENDNVQQGNNARLLRSLTNLPGRPCVLILCHPTKRAADDDLMPRGGGAFLNEVDGNIALKANDTIIGAEVQGKFRGPAFSPLAFKLDAVREHPLLKDSKGRSIPTVIARAIGEGEMASNHATQERDENKLLRLLEQHPKASLQELATLLGGAGKSKVNRLVQKLIDTKLIRREGRKIVLTQIGQKELNDLEPVVSPSFRTPFPGVSFPPLPPIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS81</accession>
    <name>Q3SS81_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cold-shock DNA-binding protein family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1599</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314864.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1599"/>
    <dbReference type="EnsemblBacteria" id="ABA04860">
        <property type="protein sequence ID" value="ABA04860"/>
        <property type="gene ID" value="Nwi_1599"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1599"/>
    <dbReference type="eggNOG" id="COG1278">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117621_4_1_5"/>
    <dbReference type="OMA" id="AHYSEIA"/>
    <dbReference type="OrthoDB" id="2034748at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012156">
        <property type="entry name" value="Cold_shock_CspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019844">
        <property type="entry name" value="CSD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002599">
        <property type="entry name" value="Cold_shock_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00050">
        <property type="entry name" value="COLDSHOCK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00352">
        <property type="entry name" value="CSD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CSD" evidence="3">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="67" mass="7503" checksum="EFD68B859D76937D" modified="2005-10-11" version="1">MQKGTVKWFNPTKGYGFIKPTTGDRDVFVHISAVERAGLSTLDENQTIEYDLVENRGKTSAENLKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSG2</accession>
    <name>Q3SSG2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1518</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04779">
        <property type="protein sequence ID" value="ABA04779"/>
        <property type="gene ID" value="Nwi_1518"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1518"/>
    <dbReference type="HOGENOM" id="CLU_2035578_0_0_5"/>
    <dbReference type="OMA" id="CERSAQS"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="121" mass="13665" checksum="202561D585BB543F" modified="2005-10-11" version="1">MHANRRSAMSRMSVGRVMCEAQTVSQFSWLDPDNFSDFRNGSPSRFWHSVCQTREAYMSEVEFERLLSAVSDVLAPRIEEAVRNAQPQVDQMGPGKLPQAANDNEVAWPFLPFPEGWNGAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSL9</accession>
    <name>Q3SSL9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TatD-related deoxyribonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1461</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. TatD-type hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314729.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1461"/>
    <dbReference type="EnsemblBacteria" id="ABA04722">
        <property type="protein sequence ID" value="ABA04722"/>
        <property type="gene ID" value="Nwi_1461"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1461"/>
    <dbReference type="eggNOG" id="COG0084">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031506_4_2_5"/>
    <dbReference type="OMA" id="PNEAPRI"/>
    <dbReference type="OrthoDB" id="915852at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004536">
        <property type="term" value="F:deoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01310">
        <property type="entry name" value="TatD_DNAse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018228">
        <property type="entry name" value="DNase_TatD-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015991">
        <property type="entry name" value="Hydrolase_TatD-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005902">
        <property type="entry name" value="DNase_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00010">
        <property type="entry name" value="TIGR00010"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01137">
        <property type="entry name" value="TATD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01090">
        <property type="entry name" value="TATD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01091">
        <property type="entry name" value="TATD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="263" mass="28522" checksum="AED5CCB56A8A72BE" modified="2005-10-11" version="1">MLVDSHCHLDFPDFADELDAVVARAEAAGVGRMVTISTRVREQAKLLAIAERFPDVYCSVGTHPHHADEEGGISIDELIALTRHPKVAALGEAGLDYFYEHGSHAAQARGFGTHIAAARETGLPLVIHTREADEDCGHILDEEMAKGAFRAVLHCYTGGRELAMKAVALGLYIGFTGILTFKKSDALRALAAELPADRILVETDAPYLAPGKFRGKRNEPSYVVETAKVLADARGVSFDDISRQTTENFFRLFSKVPHPDAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSM3</accession>
    <name>Q3SSM3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Heavy metal efflux pump CzcA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1457</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1457"/>
    <dbReference type="EnsemblBacteria" id="ABA04718">
        <property type="protein sequence ID" value="ABA04718"/>
        <property type="gene ID" value="Nwi_1457"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1457"/>
    <dbReference type="eggNOG" id="COG3696">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002755_1_2_5"/>
    <dbReference type="OMA" id="QVMMLKG"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2090.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027463">
        <property type="entry name" value="AcrB_DN_DC_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001036">
        <property type="entry name" value="Acrflvin-R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004763">
        <property type="entry name" value="CzcA/CusA/SilA/NccA/HelA/CnrA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32063">
        <property type="entry name" value="PTHR32063"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00873">
        <property type="entry name" value="ACR_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00702">
        <property type="entry name" value="ACRIFLAVINRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82714">
        <property type="entry name" value="SSF82714"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00914">
        <property type="entry name" value="2A0601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="351"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="404"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="454"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="485"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="540"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="895"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="921"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="946"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="996"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1024"/>
            <end position="1050"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1066" mass="114386" checksum="693E666A53E055F5" modified="2005-10-11" version="1">MIIDAVLAFSIRQRWLMMLFVLGIGVLGAWNFTRLPIDAVPDITNVQVQINTRAPGYSPLETEQRITFPVETAMGGLPKLDYTRSSSRYGLSQVTVVFLDGTDIYFARQLVNERIQRVRDQLPPGVEVAMGPVSTGLGEIFMYTVEARTGARTAGGGEFTPSDLRTIQDWIIKPQLRNVPGVIEVNTIGGYERQFHVLPDPGRLMAYNFGFRDVMTALAANNANVGAGYIERNGEQYLVRAPGQVANIAEIQEIVIGSRGGIPVRIRDVASVKEGRDLRTGAATMDGKETVLGTAMLLIGENSRAVARQVAAKLDEIVRSLPEGVVTRTVYDRTVLVDATIRTVQKNLIEGALLVIAVLFLILGNIRAAIVTACVIPLSMLFTITGMIETKVSANLMSLGAIDFGIIVDGAVIIVENCLRLLAEEQRRKGRILALNERLQTILDGSSEVIKPSLFGTMIIAVVYLPVLTLTGIEGKMFTPMALTVLMALLGAAILSVTFVPAAVAIVVTGKVSEKENIFMRGAKQVYLPLLDGAIRYRHMVVAVAVVIVLGSGVAATRMGGEFIPSLDEGDIAIAAIRIPGTSLTQSLDLQSTLERRIKQLPEVKDFFARTGTAEVATDAMPPSISDGYVMLKPRREWPDPSKPKSDLVEEIEAAANEIPGSSYEISQPIQLRVNELISGVRSDLGIKIFGDDMDILLGAAEQVREVIRGIAGASDVKAEQVSGLPILTVKLDRQALSRYGLSIADVQNVVEIAIGGKSAGKLFEGDRRFDIVVRLPESLRGRLESIRAISIPLPSAGIAAAEATKFAWATSPVQMRYVPLSSVAAVDVAPGPNQISRENGKRRIVVTANVRERDLGSFVADAQHAVAEKVQLPPGYWIGWGGQFEQLVSATQRLTVVVPVALALVLLLLFMSFGSVADALLVFSGVPLALTGGVLALLLRGLPLSISAGVGFIALSGVAVLNGMVIVAFIERLRREGKPVWDAVRQGASTRLRPVLMTALVASLGFVPMAIATGAGAEVQRPLATVVIGGIVSSTILTLLVLPALYILFRKDSDPAAEQPRTTHP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-09-02" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSR2</accession>
    <name>CTAA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Heme A synthase</fullName>
            <shortName evidence="1">HAS</shortName>
            <ecNumber evidence="1">1.17.99.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cytochrome aa3-controlling protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ctaA</name>
        <name type="ordered locus">Nwi_1418</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of heme O to heme A by two successive hydroxylations of the methyl group at C8. The first hydroxylation forms heme I, the second hydroxylation results in an unstable dihydroxymethyl group, which spontaneously dehydrates, resulting in the formyl group of heme A.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 A + Fe(II)-heme o + H2O = 2 AH2 + Fe(II)-heme a</text>
            <dbReference type="Rhea" id="RHEA:63388"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:60530"/>
            <dbReference type="ChEBI" id="CHEBI:61715"/>
            <dbReference type="EC" id="1.17.99.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:63389"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; heme A biosynthesis; heme A from heme O: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with CtaB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the COX15/CtaA family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.17.99.9" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314688.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSR2"/>
    <dbReference type="SMR" id="Q3SSR2"/>
    <dbReference type="STRING" id="323098.Nwi_1418"/>
    <dbReference type="EnsemblBacteria" id="ABA04679">
        <property type="protein sequence ID" value="ABA04679"/>
        <property type="gene ID" value="Nwi_1418"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1418"/>
    <dbReference type="eggNOG" id="COG1612">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017627_0_0_5"/>
    <dbReference type="OMA" id="YWWEWGH"/>
    <dbReference type="OrthoDB" id="1135592at2"/>
    <dbReference type="UniPathway" id="UPA00269">
        <property type="reaction ID" value="UER00713"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016653">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006784">
        <property type="term" value="P:heme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01665">
        <property type="entry name" value="HemeA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003780">
        <property type="entry name" value="COX15/CtaA_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023754">
        <property type="entry name" value="HemeA_Synthase_type2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23289">
        <property type="entry name" value="PTHR23289"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02628">
        <property type="entry name" value="COX15-CtaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0350">Heme biosynthesis</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000349050" description="Heme A synthase">
        <location>
            <begin position="1"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="265"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="294"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="317"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="1">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="1">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01665"/>
        </source>
    </evidence>
    <sequence length="360" mass="40581" checksum="D08BACB0A3BCD64C" modified="2005-10-11" version="1">MTRPIAQKESHLRAVRWWLVFMALLIAAMVLVGGATRLTESGLSIVEWKPVTGSLPPLSQAEWIQAFEGYKAIPQYREMNAGMTLDQFKTIFWWEWSHRLLGRFIGIAFLLPFLWFMWRGALPSDLKRRLWIIFGLGGLQGAVGWWMVASGLTERTEVSQYRLATHLVLALLIFTAIVWTLRRLSDRPPTLASIRLRVTSGVLLVLVFVQLYFGALVAGLRAGRVFNTWPDIDGSFIPSADRLFFEQPWWRNLFDNTLTVQFQHRMMAYALFAVAVLHLIDALVSRANPAIVRGALWLALAMTLQATLGILTLLYEVPIMLALVHQGVAIVVLTLAIVQAERMTEGQSARQRQELGVAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SST2</accession>
    <name>Q3SST2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Chaperone required for the assembly of F1-ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_1398</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP12 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314668.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1398"/>
    <dbReference type="EnsemblBacteria" id="ABA04659">
        <property type="protein sequence ID" value="ABA04659"/>
        <property type="gene ID" value="Nwi_1398"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1398"/>
    <dbReference type="eggNOG" id="COG5387">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047893_3_0_5"/>
    <dbReference type="OMA" id="DTVLFQY"/>
    <dbReference type="OrthoDB" id="1673094at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043461">
        <property type="term" value="P:proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011419">
        <property type="entry name" value="ATP12_ATP_synth-F1-assembly"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042272">
        <property type="entry name" value="ATP12_ATP_synth-F1-assembly_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023335">
        <property type="entry name" value="ATP12_ortho_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21013">
        <property type="entry name" value="PTHR21013"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07542">
        <property type="entry name" value="ATP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="260" mass="28373" checksum="9D86F323734661A7" modified="2005-10-11" version="1">MRESDEGSGKATPDREETARSSAPAGPRKRFYARVGVNETPEGFAILLDDRPVRTPSRNVLAAPVREIAETIAAEWDAQHDLINPMTMPLTRLANSIIDGVTVRVDAVVEDIAKYLETDLLFYRASHPDALVAREATQWDPVLQWAAEALGARFIPAEGIVHVRQPDQAVTAVRKALPTDPWVVGALHVVTTLTGSALLAIALMRGRLDADEVWAAACVDEDWNSEQWGVDEEAAVQRAVRLVDFRAAATVLRARVEPAG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-03" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSU7</accession>
    <name>RL15_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplO</name>
        <name type="ordered locus">Nwi_1383</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314657.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSU7"/>
    <dbReference type="SMR" id="Q3SSU7"/>
    <dbReference type="STRING" id="323098.Nwi_1383"/>
    <dbReference type="EnsemblBacteria" id="ABA04644">
        <property type="protein sequence ID" value="ABA04644"/>
        <property type="gene ID" value="Nwi_1383"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1383"/>
    <dbReference type="eggNOG" id="COG0200">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055188_4_0_5"/>
    <dbReference type="OMA" id="FKNINRV"/>
    <dbReference type="OrthoDB" id="1874945at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01341">
        <property type="entry name" value="Ribosomal_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="L18e/L15P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030878">
        <property type="entry name" value="Ribosomal_L15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005749">
        <property type="entry name" value="Ribosomal_L15_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001196">
        <property type="entry name" value="Ribosomal_L15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_L18e/L15P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12934">
        <property type="entry name" value="PTHR12934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="SSF52080"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01071">
        <property type="entry name" value="rplO_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00475">
        <property type="entry name" value="RIBOSOMAL_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000251532" description="50S ribosomal protein L15">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="161" mass="16900" checksum="A7EF4757AB73F6CF" modified="2005-10-11" version="1">MKLSDIADNAGSRKKRMRIGRGIGSGKGKTGGRGGKGQTARSGVRINGFEGGQMPLHRRLPKRGFNNIFRVEFAEINLDRLQDAIDAGSIDANTTINAESLVKSGVVRRAKGGVRLLGRGEIKAKLTVEVHGASKSAIAAVEKAGGTVKILAPKKDEGEAA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-02-21" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSY0</accession>
    <name>RPOB_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit beta</fullName>
            <shortName evidence="1">RNAP subunit beta</shortName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">RNA polymerase subunit beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Transcriptase subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpoB</name>
        <name type="ordered locus">Nwi_1350</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314629.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSY0"/>
    <dbReference type="SMR" id="Q3SSY0"/>
    <dbReference type="STRING" id="323098.Nwi_1350"/>
    <dbReference type="PRIDE" id="Q3SSY0"/>
    <dbReference type="EnsemblBacteria" id="ABA04611">
        <property type="protein sequence ID" value="ABA04611"/>
        <property type="gene ID" value="Nwi_1350"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1350"/>
    <dbReference type="eggNOG" id="COG0085">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000524_4_0_5"/>
    <dbReference type="OMA" id="FMTWEGY"/>
    <dbReference type="OrthoDB" id="9601at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01321">
        <property type="entry name" value="RNApol_bact_RpoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042107">
        <property type="entry name" value="DNA-dir_RNA_pol_bsu_ext_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019462">
        <property type="entry name" value="DNA-dir_RNA_pol_bsu_external_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010243">
        <property type="entry name" value="RNA_pol_bsu_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="PTHR20856"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10385">
        <property type="entry name" value="RNA_pol_Rpb2_45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02013">
        <property type="entry name" value="rpoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000224082" description="DNA-directed RNA polymerase subunit beta">
        <location>
            <begin position="1"/>
            <end position="1380"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01321"/>
        </source>
    </evidence>
    <sequence length="1380" mass="154232" checksum="E9002B52DB7B5139" modified="2005-10-11" version="1">MVQQTFTGRKRVRKFFGHIREVAEMPNLIEVQKASYDQFLMVDEPEGGRLDEGLQAVFKSVFPINDFSGASQLEFVRYEFEAPKYDVDECRQRGMTYAAPLKVTLRLIVFDIDEETGAKSVKDIKEQDVYMGDIPLMTMNGTFVVNGTERVIVSQMHRSPGVFFDHDKGKTHSSGKLLFAARVIPYRGSWLDIEFDAKDIVFARIDRRRKIPVTSLMFALGLDGEEILSTFYKKIIYKRAKSGGGSDGWRVPYDPVRFRGYSTLNDLIDADTGKVVLEAGKKLTVRAARQLQEKGLKALRMSDEELVGMYLAEDLVNPKTGEIYAEAGEEITEKSLKALNEEGYKELPLLDIDHVNVGPYIRNTLAADKNMTREDALFDIYRVMRPGEPPTLESAQNMFQSLFFDAERYDLSAVGRVKMNMRLDLDAPDTYRTLRKEDILAVIKTLVDLRDGKGEIDDIDHLGNRRVRSVGELMENQYRVGLLRMERAIKERMSSVDIDTVMPQDLINAKPAAAAVREFFGSSQLSQFMDQTNPLSEITHKRRLSALGPGGLTRERAGFEVRDVHPTHYGRICPIETPEGPNIGLINSLATFARVNKYGFVETPYRKVKDGRVTDEVVYLSAMEEGRYHVAQANLPLDARGRFTEDLVVCRHAGEVLPVTPDKVDFMDVSPKQLVSVAAALIPFLENDDANRALMGSNMQRQAVPLVRAEAPFVGTGMEGVVARDSGAAIAARRSGVIDQIDATRVVIRATEDLDPTKSGVDIYRLMKYQRSNQSTCINQRPLVKVGDIVRKGDIIADGPSTDLGELALGRNVLVAFMPWNGYNFEDSILLSERIVKEDVFTSIHIEEFEVMARDTKLGPEEITRDIPNVSEEALKSLDEAGIVYIGAEVRAGDILVGKITPKGESPMTPEEKLLRAIFGEKASDVRDTSLRVPPGVQGTIVEVRVFNRHGVDKDERALAIEREEIERLAKDRDDEQAILDRNVYGRLADLLENRQGIAGPKGFKKDTKITRAVLDEYPKSQWWLFASPNDKLMAEIEAMRKQYDESKKGLEQRFLDKVEKLQRGDELPPGVMKMVKVFVAVKRKIQPGDKMAGRHGNKGVVSKIVPIEDMPFLEDGTHADIVLNPLGVPSRMNVGQILETHLGWACAGLGRRIGQAVDAYLASAKQETKPLKETLKKVYGDNETIKSLEDHELVELGRNLRRGVPIATPVFDGAKEADIEQMLELAGMDKSGQSTVYDGRTGDPFDRKVTVGYIYMLKLHHLVDDKIHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVAGRTKVYEAIVRGDDTFEAGIPESFNVLVKEMRSLGLNVDLHNSKIGDMMPTSEAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST45</accession>
    <name>Q3ST45_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycine cleavage system H protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">gcvH</name>
        <name evidence="6" type="ordered locus">Nwi_1285</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
        <text evidence="3">Binds 1 lipoyl cofactor covalently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the GcvH family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314572.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1285"/>
    <dbReference type="EnsemblBacteria" id="ABA04546">
        <property type="protein sequence ID" value="ABA04546"/>
        <property type="gene ID" value="Nwi_1285"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1285"/>
    <dbReference type="eggNOG" id="COG0509">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097408_2_0_5"/>
    <dbReference type="OMA" id="EHEWLSG"/>
    <dbReference type="OrthoDB" id="1773485at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06848">
        <property type="entry name" value="GCS_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00272">
        <property type="entry name" value="GcvH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002930">
        <property type="entry name" value="GCV_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033753">
        <property type="entry name" value="GCV_H/Fam206"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017453">
        <property type="entry name" value="GCV_H_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11715">
        <property type="entry name" value="PTHR11715"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01597">
        <property type="entry name" value="GCV_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00527">
        <property type="entry name" value="gcvH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="5">
        <location>
            <begin position="18"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-lipoyllysine" evidence="3 4">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR617453-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA04546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="121" mass="12909" checksum="9E9DB7C5A9868EA8" modified="2005-10-11" version="1">MTVLYTTDHEWLRIEGDTATIGITGYAQSQLGDVVFVELPKVGSQLTKAQAAAVVESVKAASDVYAPITGEVLEVNEALGAEPALVNSDAEGKAWIFKLRIADRSELEGLMDEAAYKAHTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST70</accession>
    <name>Q3ST70_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1260</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314549.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1260"/>
    <dbReference type="EnsemblBacteria" id="ABA04521">
        <property type="protein sequence ID" value="ABA04521"/>
        <property type="gene ID" value="Nwi_1260"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1260"/>
    <dbReference type="HOGENOM" id="CLU_2634520_0_0_5"/>
    <dbReference type="OrthoDB" id="616258at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="77" mass="8449" checksum="2FA530A97E76F66B" modified="2005-10-11" version="1">MNAANDNLNDELIGQTRKVWQPRLGRDLSRDEAKQVAASVTGFFAILAEWSRANMPKPANDDSDVATCDDEGAHDDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STC1</accession>
    <name>Q3STC1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Maltose alpha-D-glucosyltransferase</fullName>
            <ecNumber evidence="3">5.4.99.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Maltose alpha-D-glucosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_1208</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-maltose = alpha,alpha-trehalose</text>
            <dbReference type="Rhea" id="RHEA:15145"/>
            <dbReference type="ChEBI" id="CHEBI:16551"/>
            <dbReference type="ChEBI" id="CHEBI:17306"/>
            <dbReference type="EC" id="5.4.99.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 13 family. TreS subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.16" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314499.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1208"/>
    <dbReference type="CAZy" id="GH13">
        <property type="family name" value="Glycoside Hydrolase Family 13"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04470">
        <property type="protein sequence ID" value="ABA04470"/>
        <property type="gene ID" value="Nwi_1208"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1208"/>
    <dbReference type="eggNOG" id="COG0366">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3281">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007635_1_2_5"/>
    <dbReference type="OMA" id="QEDRFPI"/>
    <dbReference type="OrthoDB" id="1573900at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047471">
        <property type="term" value="F:maltose alpha-D-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="Glyco_hydro_13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032091">
        <property type="entry name" value="Malt_amylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045857">
        <property type="entry name" value="O16G_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012810">
        <property type="entry name" value="TreS/a-amylase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012811">
        <property type="entry name" value="TreS_maltokin_C_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16657">
        <property type="entry name" value="Malt_amylase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02457">
        <property type="entry name" value="TreS_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02456">
        <property type="entry name" value="treS_nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aamy" evidence="7">
        <location>
            <begin position="27"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA04470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1094" mass="124586" checksum="8EA29E353D0B3A97" modified="2005-10-11" version="1">MNEMSHIDADRYVQTDDLWYKDAIIYQLHVKAFADSNNDGIGDFVGLTNKLDYLQELGVTALWLLPFYPSPGRDDGYDIADYGSVNPDFGTMKDFRRFISEAKRRGLRVITELVINHTSDQHRWFKRAQRSPAGSSARNWYVWSNTDKKYQGTRIIFTDTEKSNWAWDSEANAYYWHRFFSHQPDLNFDNPRVVRAVIQVMKRWLDTGVDGFRLDAIPYLCEREGTNNENLPETHAVIKHIRAALDSYAKGKVLLAEANQWPEDVQFYFGDGDECHMAYHFPLMPRIYMAIAQEDRFPITDILRQTPDIPDNCQWAMFLRNHDELTLEMVTDIERDYLWSTYAADPRARINLGIRRRLAPLMDNDRRKIELMNSLLMSLPGTPIIYYGDEIGMGDNIYLGDRNGVRTPMQWTSDRNGGFSRCDPARLYAPPVMDAVYGYESVNVESQSRSLSSLLSWMKKLISVRKSSHVFGRGTMAFVRSSNRSVLAYLRQHGDEAVLCVANLSRSAQASELDLSHWKGRSPLEMLGRTTFPDIGELPYLITLAPYGFYWFRLNEKPPSPNVAAVIVPDYETLVVPQGATWMSLARTRSIFERDVLPSHLARSRWFPERSAGAITARVPAAIPLTKDQVSRPWLSIFDVTHRGQTGRYAMPIQIRWDRLDRKNYDARNVAAVRQGSREGTLIDAAYESGLITGIIEGLRQRATFEGGNLQISYIPTERLASITEEPIENVRPVQTEQSNSTALVDQKFVVKLYRKLENGINPEVEVGRFLTDVAGYANTPALLGSVELTGPDVHAAVAIVHAFVGNQGDAWTISSAALDRFIDEQRLVGAIDTAPALEEKAAYQRYMMQLGKRVAEMHVALASRGDIEDFRPEPVNASDVAHFIDRLTARAEKTFDRLAQARLSESDRILVEEIQSLRPALHGLLAGLLPSSIQMYSIRHHGDFHLGQVLVVRDDVFIIDFEGEPRRSLSERRMKAPAARDVAGLIRSIDYSVTSAQQRVLKTEVDDDGRLAEALSAWRDEATQTFLIAYREAMSDSRLWPQDNDESEGMLRFFLLEKALYEIDYELAHRPDWLRVALDGALRVLRNDKRDRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STC5</accession>
    <name>Q3STC5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Alpha-1,4 glucan phosphorylase</fullName>
            <ecNumber evidence="10">2.4.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_1204</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Allosteric enzyme that catalyzes the rate-limiting step in glycogen catabolism, the phosphorolytic cleavage of glycogen to produce glucose-1-phosphate, and plays a central role in maintaining cellular and organismal glucose homeostasis.</text>
    </comment>
    <comment type="function">
        <text evidence="8">Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + phosphate = [(1-&gt;4)-alpha-D-glucosyl](n-1) + alpha-D-glucose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:41732"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9586"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="2.4.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 10">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the glycogen phosphorylase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.1" evidence="10"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314495.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1204"/>
    <dbReference type="CAZy" id="GT35">
        <property type="family name" value="Glycosyltransferase Family 35"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04466">
        <property type="protein sequence ID" value="ABA04466"/>
        <property type="gene ID" value="Nwi_1204"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1204"/>
    <dbReference type="eggNOG" id="COG0058">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010198_1_1_5"/>
    <dbReference type="OMA" id="KHKRTFT"/>
    <dbReference type="OrthoDB" id="137158at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008184">
        <property type="term" value="F:glycogen phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102250">
        <property type="term" value="F:linear malto-oligosaccharide phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102499">
        <property type="term" value="F:SHG alpha-glucan phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011833">
        <property type="entry name" value="Glycg_phsphrylas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000811">
        <property type="entry name" value="Glyco_trans_35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035090">
        <property type="entry name" value="Pyridoxal_P_attach_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11468">
        <property type="entry name" value="PTHR11468"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00343">
        <property type="entry name" value="Phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000460">
        <property type="entry name" value="Pprylas_GlgP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02093">
        <property type="entry name" value="P_ylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00102">
        <property type="entry name" value="PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4 10 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 9 10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10 11" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="9">
        <location>
            <position position="682"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000460-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA04466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="831" mass="93889" checksum="00CC9911097B3C88" modified="2005-10-11" version="1">MAFDVPPKRLRSLDEDTPAWLEPEDDDIVAEIKSTILSKIVLVAGKDVKHADIHDWYIAAALTLRDRIVYQWLQSDRATQNHGAKRAYYLSLEFLIGRLLTDALTNMSLMKPFRMAIEDLGLDFDELRNVEPDAALGNGGLGRLAACFMESMASLAIPARGYGIRYEHGLFRQIISNGWQEEFPEQWLLSGNPWEFERRDVVFDIQYSGRLDHAEEDDSEGRTLWIPDETIQAIAYDTPIVGWRGHHVNALRLWSARAVDPMRLDTFNSGDHLGAMSEMARAQAISKFLYPSDETPAGRELRLRQEYFFVSASLQDILNRHLHTDGDIRSLPSHAAIQLNDTHPSLAVPELIRLLMDRYHIGWNDALDITTKTISYTNHTLLPEALETWPVELFERMLPRHLDIIYRINAEHLAAAEKHFGADARSQASVSLIDETGGRRVRMGHLAFVGSHRINGVSAMHSELMRETVFADLNSLYPDRITNKTNGITFRRWLHQCNPELTSLLQDACGAAVLDEPERLRMLERLSDDPAFQQRFQTVKRTNKVALAQIIARQFGIVVDPSALFDVQIKRIHEYKRQLLNAIETIALYQAILDDPQGNWTPRVKIFAGKAAASYRQAKLIIKLINDIGNVINNDPRIRDLLKVVFIPNYNVSAAEVIVPASDLSEQISTAGMEASGTGNMKLALNGAITIGTFDGANIEIAEHVGEENIHIFGMRADEVVQRRSMGLDATDIITSSPKLARVIEAIERGAFSPDDASRFIPIAHALRFLDHYLVSADFDDYYRAQRAVDTKWASPEWTHSSILNVARMAWFSSDRTIREYAEDIWGIPVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STE6</accession>
    <name>Q3STE6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidase_M15_4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1183</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314474.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1183"/>
    <dbReference type="EnsemblBacteria" id="ABA04445">
        <property type="protein sequence ID" value="ABA04445"/>
        <property type="gene ID" value="Nwi_1183"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1183"/>
    <dbReference type="HOGENOM" id="CLU_089638_0_0_5"/>
    <dbReference type="OMA" id="LAWEKAN"/>
    <dbReference type="OrthoDB" id="2016400at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009045">
        <property type="entry name" value="Hedgehog_sig/DD-Pept_Zn-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039561">
        <property type="entry name" value="Peptidase_M15C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13539">
        <property type="entry name" value="Peptidase_M15_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55166">
        <property type="entry name" value="SSF55166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M15_4" evidence="1">
        <location>
            <begin position="104"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="166" mass="18697" checksum="1FE7069A0CB3FE50" modified="2005-10-11" version="1">MPNAAVAANVYAWPKDTTAAKNAFYGNFHAARWQQQYLIRITTPFQMYYDKKPIPSLLVNRMCAQAMLSCFNDIWNACDHDQKKVDAAGASDFGGCFNIRPIAGSKNWSNHSWACAVDLSPRSNGFNMKATLSRVVIDVFKAHGARWGGDYRGRKDPMHFEFIRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STG9</accession>
    <name>Q3STG9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Terminase_6C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314451.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1160"/>
    <dbReference type="EnsemblBacteria" id="ABA04422">
        <property type="protein sequence ID" value="ABA04422"/>
        <property type="gene ID" value="Nwi_1160"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1160"/>
    <dbReference type="eggNOG" id="COG5323">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034922_0_0_5"/>
    <dbReference type="OMA" id="FEFWALP"/>
    <dbReference type="OrthoDB" id="892377at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035421">
        <property type="entry name" value="Terminase_6C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17289">
        <property type="entry name" value="Terminase_6C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terminase_6C" evidence="1">
        <location>
            <begin position="261"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="422" mass="45653" checksum="9D70A93A46482ACF" modified="2005-10-11" version="1">MSEAELGYLARQWDVFAHPHQRPPELAPNGAPWLIWLMIGGRGAGKTRAGAEWVRAQALGLPPFAGEAVSRIALVGETEHDAREVMIEGVSGLLAVHAAHERPSWSPSRKRLEWSNGAVAQAFSADDPESLRGPQFGCAWSDEMAKWRYAEAAFDMLQFGLRLGSQPRQLITTTPRPTALLKRLMNDPASVVTRAPTQANAFNLAPTFLQGVMARYAGTRLGRQELDGEIIEDRPDALWSRALLERCRVSEAPPLQRIVVAVDPPASVTRRADACGIVAAGQAADGTVYVIADETVAGVTPAQWAAKAIALWRRWEADALVAEVNQGGDMVKAVIAEVDASVPVIAVRATRGKWLRAEPVATLYEQGRVRHAGSFAALEDEMCDFALTGLSSGRSPDRLDALVWAVTSLALVSRAQPRVRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STL3</accession>
    <name>Q3STL3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Heat shock protein Hsp20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_1116</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314409.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1116"/>
    <dbReference type="EnsemblBacteria" id="ABA04378">
        <property type="protein sequence ID" value="ABA04378"/>
        <property type="gene ID" value="Nwi_1116"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1116"/>
    <dbReference type="eggNOG" id="COG0071">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046737_4_2_5"/>
    <dbReference type="OMA" id="YNIEKRD"/>
    <dbReference type="OrthoDB" id="1523369at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06470">
        <property type="entry name" value="ACD_IbpA-B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037913">
        <property type="entry name" value="ACD_IbpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="SSF49764"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="4">
        <location>
            <begin position="30"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="152" mass="17070" checksum="922C4A2F8D643CF7" modified="2005-10-11" version="1">MRTTLDFAPLWRSTIGFDHLADFVGSTTHQAAEENYPPYNIERSGEDHYRITLAVAGFGVDDITVTAEQNTLTIEGRKPEDGRRAYLYQGIAARPFRRVFNLADYVQVKEASFRDGLLIVDLLREVPEAMKPRRISIANGAPSSQIEQKKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STS3</accession>
    <name>Q3STS3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cell division protein FtsQ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ftsQ</name>
        <name evidence="10" type="ordered locus">Nwi_1056</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Essential cell division protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell inner membrane</location>
            <topology evidence="8">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <text evidence="8">Localizes to the division septum.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the FtsQ/DivIB family. FtsQ subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314352.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1056"/>
    <dbReference type="EnsemblBacteria" id="ABA04318">
        <property type="protein sequence ID" value="ABA04318"/>
        <property type="gene ID" value="Nwi_1056"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1056"/>
    <dbReference type="eggNOG" id="COG1589">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061141_2_0_5"/>
    <dbReference type="OMA" id="KANPWIA"/>
    <dbReference type="OrthoDB" id="1270665at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090529">
        <property type="term" value="P:cell septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00911">
        <property type="entry name" value="FtsQ_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005548">
        <property type="entry name" value="Cell_div_FtsQ/DivIB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026579">
        <property type="entry name" value="FtsQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045335">
        <property type="entry name" value="FtsQ_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034746">
        <property type="entry name" value="POTRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013685">
        <property type="entry name" value="POTRA_FtsQ_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35851">
        <property type="entry name" value="PTHR35851"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03799">
        <property type="entry name" value="FtsQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08478">
        <property type="entry name" value="POTRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51779">
        <property type="entry name" value="POTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 8 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 8" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="POTRA" evidence="9">
        <location>
            <begin position="124"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA04318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="340" mass="36893" checksum="550659C3C60AB06C" modified="2005-10-11" version="1">MNGGGRLDRSLRLPGYKSVLLRASDLLKQANLLRWAASGAAILLREQIDKLKRARAERLDARARNPNRAIAFIERYVPRRLGAIMTVVVVGGSAVLGVVAGGHVDEAVAALDDTRNALANAAGFRITSVTVNGRTQLTQEEVLAAGGVNGRSSLLFLDAAGVRDSLKANPWIADATVLKLYPGALQIDITERLPFALWQENGKLAVIAADGIVLTPHVSQRFATLPLVVGKGAETRARDFLALVANYPVVNSQLKAAIFVGERRWNLRLKDGLDIRLPENDVGRALAALVKYDRENKLLSRDITAIDMRFPDRLTVRLSEEAAKAREEQLKKSSKKAGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STX6</accession>
    <name>Q3STX6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1003"/>
    <dbReference type="EnsemblBacteria" id="ABA04265">
        <property type="protein sequence ID" value="ABA04265"/>
        <property type="gene ID" value="Nwi_1003"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1003"/>
    <dbReference type="eggNOG" id="COG5481">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_175516_1_0_5"/>
    <dbReference type="OMA" id="YVIMADE"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007420">
        <property type="entry name" value="DUF465"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038444">
        <property type="entry name" value="DUF465_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04325">
        <property type="entry name" value="DUF465"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="52"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="107" mass="12392" checksum="165E4702DDF2249D" modified="2005-10-11" version="1">MNRSHGTRDLRAARAREIIREADIPTLRTALRDSSQYVIMADEEDRELGAELARLQQEHRDLDAAIDALHQSPAPDLLRLQRLKKRKLQLRDRISFIEDQITPDIIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU36</accession>
    <name>Q3SU36_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Di-heme cytochrome c peroxidase</fullName>
            <ecNumber evidence="11">1.11.1.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_0943</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
        <text evidence="6">Binds 2 heme groups.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="6">Binds 2 heme groups per subunit.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.5" evidence="11"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0943"/>
    <dbReference type="PeroxiBase" id="3542">
        <property type="entry name" value="NwDiHPOX"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04205">
        <property type="protein sequence ID" value="ABA04205"/>
        <property type="gene ID" value="Nwi_0943"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0943"/>
    <dbReference type="eggNOG" id="COG1858">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034652_3_1_5"/>
    <dbReference type="OMA" id="YKFANVG"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004130">
        <property type="term" value="F:cytochrome-c peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004852">
        <property type="entry name" value="Di-haem_cyt_c_peroxidsae"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026259">
        <property type="entry name" value="MauG/Cytc_peroxidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03150">
        <property type="entry name" value="CCP_MauG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000294">
        <property type="entry name" value="Cytochrome-c_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6 8" id="KW-0349">Heme</keyword>
    <keyword evidence="5 7 8" id="KW-0408">Iron</keyword>
    <keyword evidence="3 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0574">Periplasm</keyword>
    <keyword evidence="11" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome c" evidence="10">
        <location>
            <begin position="29"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="10">
        <location>
            <begin position="182"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="binding site" description="covalent" evidence="6">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="6">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="7">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="covalent" evidence="6">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="6">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="7">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>2</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000294-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000294-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA04205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="308" mass="33206" checksum="352D52DB0C5B7E8D" modified="2005-10-11" version="1">MGEASTWRWTLPAGVSAPPVPADNPMTAAKVELGRRLFYDADLSVDGTMSCATCHVQKHAFADSTRTRPGVTDEPGRRNVPGLANVAWFTPLNFADPAATTLEMQAATPVFGTHPVEMGMAGREAEIGRRFGRDSCYQTMFARAFPEDGGRIDFSNVARALASFERTLISHGSAWDRQRLGPEAQAGSALFARDCASCHSGSNFTDLTMHRLGPADPALADQGLFEKTGIDADRGKFRTPSLRNIALTGPWWHDGSAWTLDEAIARHGLTHGAADVAQLIAFLGALSDTEFTQRKSLAMPEDACGKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU58</accession>
    <name>Q3SU58_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Homospermidine synthase</fullName>
            <ecNumber evidence="3">2.5.1.44</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0921</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.5.1.44" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0921"/>
    <dbReference type="EnsemblBacteria" id="ABA04183">
        <property type="protein sequence ID" value="ABA04183"/>
        <property type="gene ID" value="Nwi_0921"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0921"/>
    <dbReference type="eggNOG" id="COG5310">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046538_0_0_5"/>
    <dbReference type="OMA" id="RTWCPTP"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047296">
        <property type="term" value="F:homospermidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023181">
        <property type="entry name" value="Homospermid_syn-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032095">
        <property type="entry name" value="Sacchrp_dh-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16653">
        <property type="entry name" value="Sacchrp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sacchrp_dh_NADP" evidence="1">
        <location>
            <begin position="17"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Sacchrp_dh_C" evidence="2">
        <location>
            <begin position="162"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="478" mass="53100" checksum="AA5E6FEC2E650452" modified="2005-10-11" version="1">MMSSNASSVHARITGPIVMIGFGSIGKGALPLIEKHFEFDIARFVIIDPHADDTLAKKHGVKFIRQAITRDNYRDVLVPLLTEGGGRGFCVNLSVDTGSVDLMTMCREIGSLYIDTVVEPWLGFYFDKSAGPEKRSNYALRETLLAAKRKSEGGTTAVSTCGANPGMVSWFVKQALLDIARDTGTEFNEPKSREGWAQLAHKLGVKGIHIAERDTQRARTPKPMNVFVNTWSVEGFVSEGLQPSELGWGTHEKWMPENGRKHDTGCQAAIYLLQPGANTRVRTWCPTPGAQYGFLVTHNEAISIADYLTVRDGQSVIYRPTCHYAYHPANDAVLSLHEMFGAEGKAQPKFHILEETEIVDGIDELGVLIYGHGKNAYWYGSQLSIEETRRVAPYQNATGLQVSSAVLAGMVWALENPEAGIVEADEMDFRRCLEIQRPYLGPVKGYYTDWTPLTDRPGLFPEDIDTSDPWQFRNVLVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU65</accession>
    <name>Q3SU65_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter, ATPase subunit</fullName>
            <ecNumber evidence="7">3.6.3.25</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0914</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in beta-(1--&gt;2)glucan export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.25" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314217.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0914"/>
    <dbReference type="EnsemblBacteria" id="ABA04176">
        <property type="protein sequence ID" value="ABA04176"/>
        <property type="gene ID" value="Nwi_0914"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0914"/>
    <dbReference type="eggNOG" id="COG1121">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_11_5"/>
    <dbReference type="OMA" id="IAVLHDF"/>
    <dbReference type="OrthoDB" id="1721927at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="5"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="251" mass="26943" checksum="0CCCE0E83FEAEF00" modified="2005-10-11" version="1">MVAQLQFRDVTLGYERHPAVHHLSGEVASGALLAVVGPNGAGKSTLFRGITGILKPLAGSIALGDLDIRDIAYLPQSAEIDRSFPISVFDFTGSGLWRTTGAFGRLGRHECRRIAEALAAVGLNGFENRSIGTLSGGQMQRMLFARVLLQDAGVIVLDEPFNAIDAKTSDDLLALVRRWHEEKRTVLAALHDMEMVRANFPETLLLARGAVAWGPTGQVLTAGNLSRARQMCEAFDDSAAACAVRVPPRVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU69</accession>
    <name>Q3SU69_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FrmR/RcnR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314213.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0910"/>
    <dbReference type="EnsemblBacteria" id="ABA04172">
        <property type="protein sequence ID" value="ABA04172"/>
        <property type="gene ID" value="Nwi_0910"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0910"/>
    <dbReference type="eggNOG" id="COG1937">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130332_2_3_5"/>
    <dbReference type="OMA" id="YCIDVAN"/>
    <dbReference type="OrthoDB" id="1868412at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003735">
        <property type="entry name" value="Metal_Tscrpt_repr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038390">
        <property type="entry name" value="Metal_Tscrpt_repr_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33677">
        <property type="entry name" value="PTHR33677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02583">
        <property type="entry name" value="Trns_repr_metal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="91" mass="10531" checksum="239F23944591545C" modified="2005-10-11" version="1">MRDNIKISCRKRLSRIEGQVRGLSRMVEEDRYCIDIVTQISAVRAALRRVEEEVLKDHVAHCVEHAITSGNKTDQRRKISELMAVIERASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUB5</accession>
    <name>Q3SUB5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phage Gp37Gp68</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0862</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0862"/>
    <dbReference type="EnsemblBacteria" id="ABA04126">
        <property type="protein sequence ID" value="ABA04126"/>
        <property type="gene ID" value="Nwi_0862"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0862"/>
    <dbReference type="eggNOG" id="COG4422">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1633652_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011101">
        <property type="entry name" value="DUF5131"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07505">
        <property type="entry name" value="DUF5131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="162" mass="18793" checksum="BD6B727E14392E19" modified="2005-10-11" version="1">MAETQIEWTDATWNPVAGCSIVSAGCTNCYAMEMARRLQAMNVPKYKGLTRRSGKRTVWNGVVREDRAALDIPLRWKKPRKIFVNSMSDLFHDRVTDALRRVVVPRCRRKRRVLVELPLPSHRRFLPSRRKASSKPSYVARPDRSRRRRRSASGKVGCDRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUC0</accession>
    <name>Q3SUC0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribonuclease VapC</fullName>
            <shortName evidence="5">RNase VapC</shortName>
            <ecNumber evidence="5">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Toxin VapC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">vapC</name>
        <name evidence="7" type="ordered locus">Nwi_0857</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Toxic component of a toxin-antitoxin (TA) system. An RNase.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PINc/VapC protein family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314166.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0857"/>
    <dbReference type="EnsemblBacteria" id="ABA04121">
        <property type="protein sequence ID" value="ABA04121"/>
        <property type="gene ID" value="Nwi_0857"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0857"/>
    <dbReference type="eggNOG" id="COG1487">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118482_8_0_5"/>
    <dbReference type="OMA" id="TRCAHLH"/>
    <dbReference type="OrthoDB" id="1968541at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00265">
        <property type="entry name" value="VapC_Nob1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022907">
        <property type="entry name" value="VapC_family"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0800">Toxin</keyword>
    <keyword evidence="1 5" id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="domain" description="PINc" evidence="6">
        <location>
            <begin position="3"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="140" mass="15437" checksum="9FE962C96DE87CA2" modified="2005-10-11" version="1">MSYLLDTNVVSELRKIGDGKADARVAAWVDAEEAESFFISAITILELERGVLAIQRRDAAQGARLRAWLDNHVRPEFAGRILPVDDQIATRCAHLHIPDRRNEVDALIAATALVHGLTVATRNLRDFEGTGVVVVNPWEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUJ9</accession>
    <name>Q3SUJ9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Respiratory nitrate reductase chaperone NarJ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0777</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314091.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0777"/>
    <dbReference type="EnsemblBacteria" id="ABA04042">
        <property type="protein sequence ID" value="ABA04042"/>
        <property type="gene ID" value="Nwi_0777"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0777"/>
    <dbReference type="eggNOG" id="COG2180">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_854812_0_0_5"/>
    <dbReference type="OMA" id="VETHVVF"/>
    <dbReference type="OrthoDB" id="1975985at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003765">
        <property type="entry name" value="NO3_reductase_chaperone_NarJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036411">
        <property type="entry name" value="TorD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43680">
        <property type="entry name" value="PTHR43680"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89155">
        <property type="entry name" value="SSF89155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="342" mass="38715" checksum="66A2E41BD2368CD6" modified="2005-10-11" version="1">MSRFSHRAEVLYSNRSRVLGLFADILDYPVPGLGEKASECVALIKSVQPQAAALLESFRVFAEETPVGKLQEVYSGFFDLNSICHPYVGYQLFGENYKRSVFLVELKKSYRANGFEPDPTEMPDRLSVVLRFSAQNEGDDIDALLNKGVLPGLERMTTKPETESHQHGPADIDGDTGIERAKLDDRDLRKQLKAQQREARKLLEGQSQGDDRQQLKGQGQGDVLEGGFLLAMSEDYDVEEAEKRAHPYHQVLDALRLLLNEGMTKKRADRSVEQERVLSWTRERFNLDEDETIMVSELPCSDPGCPPVETHVVFWTQSGRQHFKVYKPLAEVAADDLLDWRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUM5</accession>
    <name>Q3SUM5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HIG1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0751</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314070.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0751"/>
    <dbReference type="EnsemblBacteria" id="ABA04016">
        <property type="protein sequence ID" value="ABA04016"/>
        <property type="gene ID" value="Nwi_0751"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0751"/>
    <dbReference type="eggNOG" id="ENOG5033A2N">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_186083_2_0_5"/>
    <dbReference type="OMA" id="LINMMRG"/>
    <dbReference type="OrthoDB" id="2027635at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007667">
        <property type="entry name" value="Hypoxia_induced_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04588">
        <property type="entry name" value="HIG_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51503">
        <property type="entry name" value="HIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="HIG1" evidence="2">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="64" mass="7084" checksum="5B94EEC4172A1AFF" modified="2005-10-11" version="1">MTVFLSMILVPVAVASVAAVLMMGLVNMMRGGSPNLSQKLMRWRVFLQFVAIIIAMVTIWMLGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUN8</accession>
    <name>Q3SUN8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Resolvase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0737</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0737"/>
    <dbReference type="EnsemblBacteria" id="ABA04003">
        <property type="protein sequence ID" value="ABA04003"/>
        <property type="gene ID" value="Nwi_0737"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0737"/>
    <dbReference type="eggNOG" id="COG1961">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010686_17_1_5"/>
    <dbReference type="OMA" id="MTECKRA"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038109">
        <property type="entry name" value="DNA_bind_recomb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011109">
        <property type="entry name" value="DNA_bind_recombinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006118">
        <property type="entry name" value="Recombinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025827">
        <property type="entry name" value="Zn_ribbon_recom_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07508">
        <property type="entry name" value="Recombinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13408">
        <property type="entry name" value="Zn_ribbon_recom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="SSF53041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00397">
        <property type="entry name" value="RECOMBINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="6">
        <location>
            <begin position="8"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-serine intermediate" evidence="4 5">
        <location>
            <position position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606118-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="542" mass="61175" checksum="F95619FBAA444F6F" modified="2005-10-11" version="1">MKATTSQKAVIYCRVSSKKQVREGDGLRSQETRCREYAAYKGYEIVGVFCDSVTGSKKKRLGLSAVVKHLKAHRKDGCVVIIDHIDRFARDVRWHWDLRDLLREAGGKLESPSIEFGEDADSILRENLLASVSQHQRQKNAEQTRNRMRARAMNGYWVFQAPMGYRYERVAGHGKMLVPQEPLASIIGEALEGYASGRFEGLTEVKRFLESQPAFPRNSNDEVHIERISEIFSRPVYAGHITIEDWGLRLVPGKHKQLVSLATWQAVQDRRSGTAKAPARADISEDFPLRGFVSCGCCGKPLTACWSKGRSARYPYYLCDTRGCPESRKSIRRDAIEGEFETLLTSLKPTEGLFNVAFAMFRDLWEAKLASQHSQGGTLKKEIALIERKVDALLDRIVDADSDSIVRAYEKRIRDLETQKALMRDRIANCGRPLTSFSEAYRTAFDFLANPCKLWHSEKIEDRRAVLKLAFAERLIYVRGEGYRTAQFSMPFKLIGGAEMNEKEMVPLAGIEPALLAELDFESSASTSSATGAFASHIRMRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUU6</accession>
    <name>Q3SUU6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GBBH-like_N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0679</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314001.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0679"/>
    <dbReference type="EnsemblBacteria" id="ABA03945">
        <property type="protein sequence ID" value="ABA03945"/>
        <property type="gene ID" value="Nwi_0679"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0679"/>
    <dbReference type="eggNOG" id="COG3536">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117841_2_0_5"/>
    <dbReference type="OMA" id="RLDVIWE"/>
    <dbReference type="OrthoDB" id="2003927at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2020.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010376">
        <property type="entry name" value="GBBH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038492">
        <property type="entry name" value="GBBH-like_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06155">
        <property type="entry name" value="GBBH-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GBBH-like_N" evidence="3">
        <location>
            <begin position="10"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="97" mass="10948" checksum="339C527E4EB89A02" modified="2005-10-11" version="1">MQPTNVELVEGATVLALTWHDCGVRRLEAERLRRASRAATEIRRQADGVELYLPPDLHVTSIEAIGNYALRLSFSDGHDRGIYPWCYLRELAGQTPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUV1</accession>
    <name>Q3SUV1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC transporter sulfonate/nitrate transport system substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0674</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0674"/>
    <dbReference type="EnsemblBacteria" id="ABA03940">
        <property type="protein sequence ID" value="ABA03940"/>
        <property type="gene ID" value="Nwi_0674"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0674"/>
    <dbReference type="eggNOG" id="COG0715">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028871_8_0_5"/>
    <dbReference type="OMA" id="MGDYPLV"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015168">
        <property type="entry name" value="SsuA/THI5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09084">
        <property type="entry name" value="NMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NMT1" evidence="1">
        <location>
            <begin position="147"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="474" mass="51700" checksum="3645BAE02D676721" modified="2005-10-11" version="1">MVRWSNLISRDNSMISFRVANMTAGIATAMISMLGATNIVHAEAITIGIGHQSMCTDTYAAGIIVKELKLLEKHLPRSGKYVDVTYNLKWDDYASGGPITNQMLAGKLDIGVMGDYPVIVNGAKFQATKSLNTSLVSSTGYNLRGAGNAIVVPASSNVYSLAQLKGRSISTPYGSAAWGMLIKALEDAHISLSDVDLKNQAPSVGAANIATGKIDAHADFCPWSEIMEYRGTGRKIYDGSQTGVPYLHGVVVRKDFVDKYPEIIVAFIKAIHDAGEWLKKDPALAAAQMEKWTGVEKEVLYLYYSRGGHLTVDPTLKPQWIETLEFDHGVLARADLSPPLDFKEWVDDRYVKKAYAELGLDYAAQSKEIVDPLIANRDLPNEIWHAKDGILSYPTTAAFLKAAADFQATGVKINAAYVYDHDTGLKLFGTTAFYVRNKDGSYTAFLRKDPAETYAAKTGGEVSTFTEAIARQAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUZ2</accession>
    <name>Q3SUZ2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Hemolysin A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0632</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TlyA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313959.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0632"/>
    <dbReference type="EnsemblBacteria" id="ABA03899">
        <property type="protein sequence ID" value="ABA03899"/>
        <property type="gene ID" value="Nwi_0632"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0632"/>
    <dbReference type="eggNOG" id="COG1189">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058015_1_0_5"/>
    <dbReference type="OMA" id="VLMVKPQ"/>
    <dbReference type="OrthoDB" id="1670446at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004538">
        <property type="entry name" value="Haemolysin_A/TlyA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32319:SF0">
        <property type="entry name" value="PTHR32319:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005578">
        <property type="entry name" value="TlyA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00478">
        <property type="entry name" value="tly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="S4" evidence="4">
        <location>
            <begin position="13"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00029460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="251" mass="26544" checksum="0391E985F69AC080" modified="2005-10-11" version="1">MANREKSEPRARKRADVLLVERGLFESRARAQAAIEAGFVTADGKPVTKVSEAVDVSAAIEAQPAHPYVSRGGVKLAGALAAYPVEIEDHVCLDVGASTGGFTEVLLSEGAALVFAVDVGHDQLHPSLRGDPRVVSMESTDIRSLEGKRLPQRPNVVVMDASFISLKAILPAALALAAAQMHLLALIKPQFEAPRAAIRKGIVRDAAVHRAVCDDIAAFASTLGCSHIEIFPSPIRGGDGNIEFFLGARRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV91</accession>
    <name>Q3SV91_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hemolysin-type calcium-binding region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0533</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313861.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0533"/>
    <dbReference type="EnsemblBacteria" id="ABA03800">
        <property type="protein sequence ID" value="ABA03800"/>
        <property type="gene ID" value="Nwi_0533"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0533"/>
    <dbReference type="eggNOG" id="COG2931">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_629577_0_0_5"/>
    <dbReference type="OMA" id="HNFPSIN"/>
    <dbReference type="OrthoDB" id="1361614at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.150.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018511">
        <property type="entry name" value="Hemolysin-typ_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001343">
        <property type="entry name" value="Hemolysn_Ca-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011049">
        <property type="entry name" value="Serralysin-like_metalloprot_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00353">
        <property type="entry name" value="HemolysinCabind"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51120">
        <property type="entry name" value="SSF51120"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00330">
        <property type="entry name" value="HEMOLYSIN_CALCIUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="359" mass="36228" checksum="2869B74D357EE73D" modified="2005-10-11" version="1">MAVSYLTKTELGEFFHSNGNIEAGVRSALIDSLEQSGVFNDDDNSARGWLQSGPFPGGEVPPVVQILGVSNSTNVETNPNLKAIVLDDHSARLDVTGGDNDVFVAAGKGHDTINLNDSGNDTVYGGSGNDVIRASEGDNLLFGGAGNDSIYGGAGNDTLDGGAGNDLLVSGGARAAGVGGDARIASADDSAFGAAGRGHHHGGGHGDDRGWGRGDDHGGGRGGDHGGGVGEIEGHSTLIGGAGNDTLVGMQGDVLQGGAGNDQFTLLSGDGSANSTLYGGGGDDTFRIEARSGSDTIFGGNGNDTAEFVGRSFFDVAKIDVDASTSTYTLHFSDQQTVAVNGVEELHFSDQIVTLPKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVA0</accession>
    <name>Q3SVA0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein-glutamate O-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.80</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_0524</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamyl-[protein] + S-adenosyl-L-methionine = [protein]-L-glutamate 5-O-methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:24452"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:10311"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:82795"/>
            <dbReference type="EC" id="2.1.1.80"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.80" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313852.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0524"/>
    <dbReference type="EnsemblBacteria" id="ABA03791">
        <property type="protein sequence ID" value="ABA03791"/>
        <property type="gene ID" value="Nwi_0524"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0524"/>
    <dbReference type="eggNOG" id="COG1352">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025854_0_2_5"/>
    <dbReference type="OMA" id="PGIIYKA"/>
    <dbReference type="OrthoDB" id="1378151at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008983">
        <property type="term" value="F:protein-glutamate O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.155.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022642">
        <property type="entry name" value="CheR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000780">
        <property type="entry name" value="CheR_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022641">
        <property type="entry name" value="CheR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036804">
        <property type="entry name" value="CheR_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01739">
        <property type="entry name" value="CheR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03705">
        <property type="entry name" value="CheR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00996">
        <property type="entry name" value="CHERMTFRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00138">
        <property type="entry name" value="MeTrc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50123">
        <property type="entry name" value="CHER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CheR-type methyltransferase" evidence="7">
        <location>
            <begin position="1"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="260"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA03791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="288" mass="32210" checksum="21CD674D728A6C41" modified="2005-10-11" version="1">MTPLDFEYLRKLLKERSGLDLTAEKQYLVESRLTPLARKAGLSGIGDLVQKMKTGATALTIDVVEAMTTNETFFFRDKTPFEHLRETVIPALLQARAARRHLRIWCAAGSTGQEPYSIAMCLKECGPKLTGWRTEIIATDLSNEVLEKSRAGLYSQFEVQRGLPIQLLIKHFKQVGELWQINPDIRAMVQHRQLNLLQDFSHLGVFDVIFCRNVLIYFDQETKAGVFTRLARQVEADGFLALGTAETVVGLTDAFKPHPDKRGLYRPNRVSPPSRAAGMPSPRAAAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVA7</accession>
    <name>Q3SVA7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0517</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313845.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0517"/>
    <dbReference type="EnsemblBacteria" id="ABA03784">
        <property type="protein sequence ID" value="ABA03784"/>
        <property type="gene ID" value="Nwi_0517"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0517"/>
    <dbReference type="eggNOG" id="COG3350">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087914_1_0_5"/>
    <dbReference type="OMA" id="WCAYAMG"/>
    <dbReference type="OrthoDB" id="1898704at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="168" mass="18322" checksum="191BBDDB17A39B3E" modified="2005-10-11" version="1">MTARRQERNGLRLGLAVIAALGGGLAPPGLDLTALAATSERVVVDRYTGLAIGGFDPVAFFTDARPVRGVSEFEVSHDGVIWRFANPSNRAFFLADPDVYSPRFGGYDPVGVTRGVVRAGTARLWLVIGERLYLFGHEDTRAAFAADPARYLREATRRWAALKEMLAQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-05-25" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVI0</accession>
    <name>PROA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Gamma-glutamyl phosphate reductase</fullName>
            <shortName evidence="1">GPR</shortName>
            <ecNumber evidence="1">1.2.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glutamate-5-semialdehyde dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glutamyl-gamma-semialdehyde dehydrogenase</fullName>
            <shortName evidence="1">GSA dehydrogenase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">proA</name>
        <name type="ordered locus">Nwi_0444</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NADPH-dependent reduction of L-glutamate 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamate 5-semialdehyde + NADP(+) + phosphate = H(+) + L-glutamyl 5-phosphate + NADPH</text>
            <dbReference type="Rhea" id="RHEA:19541"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="ChEBI" id="CHEBI:58274"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.41"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the gamma-glutamyl phosphate reductase family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="ABA03711" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="1.2.1.41" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03711.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVI0"/>
    <dbReference type="SMR" id="Q3SVI0"/>
    <dbReference type="STRING" id="323098.Nwi_0444"/>
    <dbReference type="EnsemblBacteria" id="ABA03711">
        <property type="protein sequence ID" value="ABA03711"/>
        <property type="gene ID" value="Nwi_0444"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0444"/>
    <dbReference type="eggNOG" id="COG0014">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030231_0_0_5"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00360"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004350">
        <property type="term" value="F:glutamate-5-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07079">
        <property type="entry name" value="ALDH_F18-19_ProA-GPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00412">
        <property type="entry name" value="ProA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020593">
        <property type="entry name" value="G-glutamylP_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012134">
        <property type="entry name" value="Glu-5-SA_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000965">
        <property type="entry name" value="GPR_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000151">
        <property type="entry name" value="GPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00407">
        <property type="entry name" value="proA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01223">
        <property type="entry name" value="PROA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0641">Proline biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000230006" description="Gamma-glutamyl phosphate reductase">
        <location>
            <begin position="1"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="426" mass="44718" checksum="2506035349E29735" modified="2006-04-04" version="2">MKAIGAPADLPVLMGDLAAQAREAARSLGLASTAQKNEALDAMARAIRSNAAAILQANAQDVADARAGGATAAFLDRLTLTQARVEAMADGVKVVREIDDPVGRVTERWQRPNGMTIERVRVPLGVVAVIFESRPNVCADAGVLCLKSGNAVILRGGSESFRSCRAIHACLVEGLREARLPEAAITLVPTRDRAAVGLLLSGMNGGIDVIVPRGGKSLVARVEAEARVPVFAHLEGVNHVYVDGAADLAMAKAVVLNSKMRRPGVCGAAETLLVDRSGVRDILKPLVEALIEAGCEVRGDSDVQGVDPRVRSAADSDWNTEYGDAIISARVVDGCGGAIDHIQRHGSRHTDAIVTEDPETAARFLNEVDSAIVLHNASTQFADGGEFGFGAEIGIATGKFHARGPVGAEQLTSFKYRVHGDGQVRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVL3</accession>
    <name>Q3SVL3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ferredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_0411</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 7">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313742.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0411"/>
    <dbReference type="EnsemblBacteria" id="ABA03678">
        <property type="protein sequence ID" value="ABA03678"/>
        <property type="gene ID" value="Nwi_0411"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0411"/>
    <dbReference type="eggNOG" id="COG1146">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_0_0_5"/>
    <dbReference type="OMA" id="DCIHPKK"/>
    <dbReference type="OrthoDB" id="1873445at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000813">
        <property type="entry name" value="7Fe_ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022569">
        <property type="entry name" value="Fd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11953">
        <property type="entry name" value="DUF3470"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00354">
        <property type="entry name" value="7FE8SFRDOXIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4 7" id="KW-0408">Iron</keyword>
    <keyword evidence="5 7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="9">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="9">
        <location>
            <begin position="31"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="78"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="85"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA03678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="112" mass="12548" checksum="56C46FCAC5F1A90C" modified="2005-10-11" version="1">MTYVVNDACIKCKYTDCVEVCPVDCFYEGENMLVIHPDECIDCGVCEPECPAEAIKPDSEPGVEKWLEVNAEYAGRWPNITQKKETPPDAKDFEGQEGKFEKYFSPDPGTGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVM4</accession>
    <name>Q3SVM4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, BolA protein family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BolA/IbaG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313731.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0400"/>
    <dbReference type="EnsemblBacteria" id="ABA03667">
        <property type="protein sequence ID" value="ABA03667"/>
        <property type="gene ID" value="Nwi_0400"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0400"/>
    <dbReference type="eggNOG" id="COG0271">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109462_2_1_5"/>
    <dbReference type="OMA" id="TRVEIHR"/>
    <dbReference type="OrthoDB" id="1981704at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002634">
        <property type="entry name" value="BolA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036065">
        <property type="entry name" value="BolA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01722">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003113">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82657">
        <property type="entry name" value="SSF82657"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="91" mass="10066" checksum="010AB720AA6B5C61" modified="2005-10-11" version="1">MSVKDNISKKLSEAFAPESLDVADESYLHEGHAGHRPGGETHFRIHIVSQAFEGKSRVERHRMINTLLAAELDGPIHALAIRAMAPGEQQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVN5</accession>
    <name>Q3SVN5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Parvulin-like PPIase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Peptidyl-prolyl cis-trans isomerase plp</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Rotamase plp</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_0389</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PpiC/parvulin rotamase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313720.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0389"/>
    <dbReference type="EnsemblBacteria" id="ABA03656">
        <property type="protein sequence ID" value="ABA03656"/>
        <property type="gene ID" value="Nwi_0389"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0389"/>
    <dbReference type="eggNOG" id="COG0760">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034646_1_3_5"/>
    <dbReference type="OMA" id="YEQAKPT"/>
    <dbReference type="OrthoDB" id="1838755at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000297">
        <property type="entry name" value="PPIase_PpiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027304">
        <property type="entry name" value="Trigger_fact/SurA_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109998">
        <property type="entry name" value="SSF109998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50198">
        <property type="entry name" value="PPIC_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PpiC" evidence="9">
        <location>
            <begin position="149"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="277"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA03656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="301" mass="33106" checksum="7AF78F97B401AFC1" modified="2005-10-11" version="1">MTISLPATKHFLRVRLVSSVLGGCLAAVLLVGTPVHADDANPVLAKVNGSEIRQSDVNLAAEELGPSLAQMDPAAKQENVLSFLIDMKIIAKAAEEKKVENSEDFKKRLAFARDRLLMDRLLASEGKAALTDSAMKTVYTDASKQITSEQEVHARHILVPTEEEAKKVEEELKKGADFAELAKKESKDPGASDGGDLGFFTKEQMVPDFSKVAFALEPGKISDPVKTQFGWHIIKVEEKRARKAPDFDQVKPQIEQFVTRKAQAEYVAKLRETAKIERLDKPADDKTKSAKPADDKTPAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVR8</accession>
    <name>Q3SVR8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Lysine exporter protein (LYSE/YGGA)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_0356</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0356"/>
    <dbReference type="EnsemblBacteria" id="ABA03623">
        <property type="protein sequence ID" value="ABA03623"/>
        <property type="gene ID" value="Nwi_0356"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0356"/>
    <dbReference type="eggNOG" id="COG1280">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079569_3_2_5"/>
    <dbReference type="OMA" id="LHMACCA"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001123">
        <property type="entry name" value="LeuE-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30086">
        <property type="entry name" value="PTHR30086"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01810">
        <property type="entry name" value="LysE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006324">
        <property type="entry name" value="LeuE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="181"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA03623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="203" mass="21407" checksum="48A273CAB7729277" modified="2005-10-11" version="1">MLAFALTSLVIEMTPGPNMAYLAALSLSQGIRAGLAAVGGVALGLAVYGLVAALGLSAAIDSSPLLYEVLRWGGVVYLLWLAWEAWARETETSAGNNGDPDIRPAQAFRRGLITNLLNPKAAVFYVAVLPDFVQIGKGSVAMQTLTLSAVYVGVATVVHLAIVLLASRLQSAIRTADKRRTVRRVLAVALAAIAIWFAFSTAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="127" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVT7</accession>
    <name>Q3SVT7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Potassium/proton antiporter, CPA1 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0337</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313669.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0337"/>
    <dbReference type="EnsemblBacteria" id="ABA03604">
        <property type="protein sequence ID" value="ABA03604"/>
        <property type="gene ID" value="Nwi_0337"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0337"/>
    <dbReference type="eggNOG" id="COG3263">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005912_9_2_5"/>
    <dbReference type="OMA" id="QIGMFVL"/>
    <dbReference type="OrthoDB" id="1194802at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005451">
        <property type="term" value="F:monovalent cation:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030151">
        <property type="entry name" value="NhaP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005170">
        <property type="entry name" value="Transptr-assoc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32507:SF7">
        <property type="entry name" value="PTHR32507:SF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03471">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01091">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0630">Potassium</keyword>
    <keyword evidence="1" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="62"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="232"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="270"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="304"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="336"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="363"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="6">
        <location>
            <begin position="404"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="597" mass="63692" checksum="261EE44CC478AAE1" modified="2005-10-11" version="1">MASLDSVSLAILLGAILVMAGILSSLLALRFGAPLLLVFLFIGMLAGDSGPGGLSFSDVQNTYLVGSAALALILFDGGLKTRFQSIRTVLAPSMVLATLGVLLTALVTAPVARYALDLNWTEALLIGAVVASTDAAAVFLLVHAQGLRLRPRVGATLEAESGTNDPFAVFLTLMLVELISVGDSSVWHVVIEFLRESMLGGIVGVIGGRLVVVALNRVALPQGLHAPFVTTAALVIFGVAQIFHASGFLAVYLAGIIIGNRPTRAHSSVVTFLDAATWLAQIVMFVLLGLLVSPYRLMDSALPAVLVAFVLMLVARPLAVFLCLAPFPFNWREKLFIAWTGLRGAVAIFLASIPMLVGLSKAYLYFDVAFVVVIISLLLQGWTLAPAARRLHVALPRADRGPRRVELDLPGQLEQQLVGYAVRPTSLFLRRGLIPSWSKPTLVIRNEKILTPAEAEPIEAGDYLYLLAPPEKAEALDRFFVDMQPMASPDPHLLGDFLVSGETSLGDIAQIYGVSVDPAAAELTLADYFDVHLDRAPKEGATLPLDAIVLVARNISQGRVNAVGLRLPQDEEKRPAPSRMGTLKQKAAKAWSAVAGT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-28" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW30</accession>
    <name>BIOB_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Biotin synthase</fullName>
            <ecNumber evidence="1">2.8.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">bioB</name>
        <name type="ordered locus">Nwi_0243</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(4R,5S)-dethiobiotin + [sulfur carrier]-SH + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2 5'-deoxyadenosine + [sulfur carrier]-H + biotin + 2 L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:22060"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:149473"/>
            <dbReference type="EC" id="2.8.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="1">Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the radical SAM superfamily. Biotin synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.6" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313577.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SW30"/>
    <dbReference type="SMR" id="Q3SW30"/>
    <dbReference type="STRING" id="323098.Nwi_0243"/>
    <dbReference type="EnsemblBacteria" id="ABA03511">
        <property type="protein sequence ID" value="ABA03511"/>
        <property type="gene ID" value="Nwi_0243"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0243"/>
    <dbReference type="eggNOG" id="COG0502">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033172_1_2_5"/>
    <dbReference type="OMA" id="AMGVHRY"/>
    <dbReference type="OrthoDB" id="940969at2"/>
    <dbReference type="UniPathway" id="UPA00078">
        <property type="reaction ID" value="UER00162"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004076">
        <property type="term" value="F:biotin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01694">
        <property type="entry name" value="BioB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010722">
        <property type="entry name" value="BATS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002684">
        <property type="entry name" value="Biotin_synth/BioAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024177">
        <property type="entry name" value="Biotin_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22976">
        <property type="entry name" value="PTHR22976"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06968">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001619">
        <property type="entry name" value="Biotin_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00272">
        <property type="entry name" value="biotin_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00876">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00433">
        <property type="entry name" value="bioB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0001">2Fe-2S</keyword>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0093">Biotin biosynthesis</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000381507" description="Biotin synthase">
        <location>
            <begin position="1"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="53"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <sequence length="341" mass="37433" checksum="224545513FF7C5C0" modified="2005-10-11" version="1">MKSDPVSFDSSHDLMARPRHDWTRVEAETLYTTPFPDLLFQAQTIHRRHFDPNHVETASLLSIKTGGCPEDCGYCAQSAHYDAGVKATKLMDKDAVLETARRAKDAGAGRFCMAAAWRHPKDRDLDRVCDMISAVKALGMETCATLGMLTPEQARRLQEAGLDFYNHNVDTSPEFYDKIITTRTMQDRIETLACAREAGLKLCCGGIIGMGEQVGDRLGMLILLANLDEHPESVPINLWNEVRGVPVNDTADRPDPIALVRMIAVARIMMPKSVVRLSAGRQYMTDELQALCFVAGANSIFIGDVLLTTKNPATLRDAALLDRLGMSSPLDGVKMPAASPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW86</accession>
    <name>Q3SW86_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATP dependent helicase, Lhr family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0187</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313523.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0187"/>
    <dbReference type="EnsemblBacteria" id="ABA03455">
        <property type="protein sequence ID" value="ABA03455"/>
        <property type="gene ID" value="Nwi_0187"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0187"/>
    <dbReference type="eggNOG" id="COG1201">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002025_0_1_5"/>
    <dbReference type="OMA" id="VVDEWHE"/>
    <dbReference type="OrthoDB" id="35275at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013701">
        <property type="entry name" value="DEAD/DEAH_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026362">
        <property type="entry name" value="DEXH_lig_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017170">
        <property type="entry name" value="Lhr-like_ATP-dep_RNA_helic_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045628">
        <property type="entry name" value="Lhr_WH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08494">
        <property type="entry name" value="DEAD_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19306">
        <property type="entry name" value="Lhr_WH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037307">
        <property type="entry name" value="Lhr-like_helic_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04121">
        <property type="entry name" value="DEXH_lig_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="35"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="2">
        <location>
            <begin position="247"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="835" mass="92915" checksum="05EBE9A40080CD14" modified="2005-10-11" version="1">MEIADRETQPLLPEGFLRWFAARRWSPRAHQLALLRKARAGRSALLIAPTGAGKTLAGFLPTLVEISGKEKHLHTLYISPLKALAVDIARNLETPIAEMNLPIRVETRTGDTPVSRRQRQRRYPPDILLTTPEQIALLLASDDAPFLFSSLRRVVLDELHALAISKRGDLLSLALARLWRLAPRMRTTGLSATVADPHALARFLAPQSRDKAVSADIVEADGAAAPVVEMLDTSERLPWAGHSARHALTEVYDLIRRNRTTLVFVNTRSQAEMLFQDLWRINDDGLAIALHHGSLDVAQRRKVEQAMTAGRLRGVVCTSSLDLGVDWGDVDLVINIGAPKGASRLMQRIGRANHRLDEASRAVLVPANRFEVLECRAAIDAVNENAQDTPPLRTGALDVLAQHVMGRACGAPFHADDLYEEVRTAAPYAALARADFDDVIDFVATGGYALKSYERFARIRQDEAGRWRIANPRARQSYRLNVGTIVEEAMLKVRLVRSRHGGGKGPTGAIARGGRMLGEIEEYFIEGLVTGDTFVFGGEIVRFEALAEDQVYVSRASDESPKVPSYMGGKFPLSTYLAERVRRLLDDERAWRGLPDQVREWLKLQSRFSRVPHAREMIVETFPRAGKHYLVCYPFEGRLAHQTLGMLLTRRLERARARPLGFVANEYALAVWMLGDPSSMIRQGRLNLDALFDPDMLGDDLEAWLAESALMKRTFRSCAIISGLIARRFTGAEKSRRQVLFSTDLVYDVLRKHQPDHVLLRAARADAATGLLDIRRLGDMLARIRGRIVHKPLDRISPLAVPVMLGIGRETVYGEASDELLAEAAEQLVREATGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW95</accession>
    <name>Q3SW95_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosome hibernation promoting factor</fullName>
            <shortName evidence="2">HPF</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">hpf</name>
        <name evidence="5" type="ordered locus">Nwi_0178</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Required for dimerization of active 70S ribosomes into 100S ribosomes in stationary phase; 100S ribosomes are translationally inactive and sometimes present during exponential growth.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with 100S ribosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HPF/YfiA ribosome-associated protein family. Long HPF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313515.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0178"/>
    <dbReference type="EnsemblBacteria" id="ABA03446">
        <property type="protein sequence ID" value="ABA03446"/>
        <property type="gene ID" value="Nwi_0178"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0178"/>
    <dbReference type="eggNOG" id="COG1544">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071472_0_1_5"/>
    <dbReference type="OMA" id="RRLKDHH"/>
    <dbReference type="OrthoDB" id="1766263at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044238">
        <property type="term" value="P:primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00552">
        <property type="entry name" value="RaiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00839">
        <property type="entry name" value="HPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034694">
        <property type="entry name" value="HPF_long/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036567">
        <property type="entry name" value="RHF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003489">
        <property type="entry name" value="RHF/RaiA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032528">
        <property type="entry name" value="Ribosom_S30AE_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038416">
        <property type="entry name" value="Ribosom_S30AE_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16321">
        <property type="entry name" value="Ribosom_S30AE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02482">
        <property type="entry name" value="Ribosomal_S30AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69754">
        <property type="entry name" value="SSF69754"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00741">
        <property type="entry name" value="yfiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="1 2" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="Ribosom_S30AE_C" evidence="4">
        <location>
            <begin position="135"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="73"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="198" mass="21455" checksum="82F0A0ED196B7F35" modified="2005-10-11" version="1">MTLRISGKSINVGEALRGRLNSRTDEVLRKYFDGQYSGHITLSKDGFGFRTDCALHLDSGVTLEAESNAADAYASADQALLQIEKRLRRYKSRLKDHSARKARAAAETIAGLDAPSYVIEAPAMNGEGEVTAFNPVIIAETITSLRRLSVSEAVMELDFTGAPVLVFQHGSSGRVNMIYRRPDGNVGWIDPPAVNGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWC8</accession>
    <name>Q3SWC8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Possible glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0145</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0145"/>
    <dbReference type="EnsemblBacteria" id="ABA03413">
        <property type="protein sequence ID" value="ABA03413"/>
        <property type="gene ID" value="Nwi_0145"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0145"/>
    <dbReference type="eggNOG" id="COG1215">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1395742_0_0_5"/>
    <dbReference type="OMA" id="YWACLSI"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13632">
        <property type="entry name" value="Glyco_trans_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="73"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="3">
        <location>
            <begin position="2"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="216" mass="24120" checksum="0BD0EBA10FB7BA21" modified="2005-10-11" version="1">MGGWDAYNVTEDADLGFRLARFGYRSTTFASTTFEEAPARFGGWLRQRSRWMKGWMQTWSVHMRRPRQLWKDAGAKGFLTLNAIIGGNVLTALTFPILMIDMALRSLEGAGGGFAAGAVTPLHLTTIAAGFVSTIVVGLMGLARRGQLRQGWILALTPIYWACLSIAAWRALYQLLSEPYRWEKTEHGLSPQIRSAATSQGTASHHMAARRRQRYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWD5</accession>
    <name>Q3SWD5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Lysophospholipase L1-like esterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0138</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0138"/>
    <dbReference type="EnsemblBacteria" id="ABA03406">
        <property type="protein sequence ID" value="ABA03406"/>
        <property type="gene ID" value="Nwi_0138"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0138"/>
    <dbReference type="eggNOG" id="COG2755">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065317_0_0_5"/>
    <dbReference type="OMA" id="EVMRDWH"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229200" evidence="1">
        <location>
            <begin position="31"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="SGNH_hydro" evidence="2">
        <location>
            <begin position="121"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="327" mass="34345" checksum="B936C0E19D51EF5C" modified="2005-10-11" version="1">MMVNSRIFRTAGPVALALMLAGAAASRAVAQTAPANAAALPGSSAELSASPSLGAATAGTGMPAPGLSGTVADKTAAMAASVRDIFDRVPCLPPKGISQSTGALPHVAAKLAAHKAVVIVAFGSSSTQGHGATSPEFNYPNRLAAQLHRKYPAAAITVLNRGKGGEDAPEMIKRLRTEVIDAKPDMVIWQVGTNAVLRDLDPASTVKTVEDGVATIQASGADLVLVDPQYAPAVNARGETADRMIRLLNKVAHHRHVGIFPRFEVMRDWHDRQSLPFDTFVTKDGLHMNDWGYACFAQLLGDDIIRSVDQVQAGVRLQPAATVNRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWF8</accession>
    <name>Q3SWF8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal RNA small subunit methyltransferase I</fullName>
            <ecNumber evidence="6">2.1.1.198</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">16S rRNA 2'-O-ribose C1402 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">rRNA (cytidine-2'-O-)-methyltransferase RsmI</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rsmI</name>
        <name evidence="8" type="ordered locus">Nwi_0115</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the 2'-O-methylation of the ribose of cytidine 1402 (C1402) in 16S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>cytidine(1402) in 16S rRNA + S-adenosyl-L-methionine = 2'-O-methylcytidine(1402) in 16S rRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42924"/>
            <dbReference type="Rhea" id="RHEA-COMP:10285"/>
            <dbReference type="Rhea" id="RHEA-COMP:10286"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.198"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the methyltransferase superfamily. RsmI family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.198" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313452.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0115"/>
    <dbReference type="EnsemblBacteria" id="ABA03383">
        <property type="protein sequence ID" value="ABA03383"/>
        <property type="gene ID" value="Nwi_0115"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0115"/>
    <dbReference type="eggNOG" id="COG0313">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044779_2_1_5"/>
    <dbReference type="OMA" id="RTMVFFE"/>
    <dbReference type="OrthoDB" id="1059309at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070677">
        <property type="term" value="F:rRNA (cytosine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000453">
        <property type="term" value="P:enzyme-directed rRNA 2'-O-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11648">
        <property type="entry name" value="RsmI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01877">
        <property type="entry name" value="16SrRNA_methyltr_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008189">
        <property type="entry name" value="rRNA_ssu_MeTfrase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018063">
        <property type="entry name" value="SAM_MeTrfase_RsmI_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46111">
        <property type="entry name" value="PTHR46111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005917">
        <property type="entry name" value="MTase_YraL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00096">
        <property type="entry name" value="TIGR00096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01296">
        <property type="entry name" value="RSMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="7">
        <location>
            <begin position="36"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA03383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="317" mass="33614" checksum="5E808096FF050214" modified="2005-10-11" version="1">MRAKSASPKFHIETPRIRTFSIAGHQLAAPAAAPGLHLVATPIGHLGDITLRALETLAGVDFIACEDTRITRRLTERYAITGLLKPYHEHNAATARPKILAQLAQGASIALVSDAGTPLISDPGFKLVRDVCAAGHQVIALPGASSVLAALSVAALPTDRFFFEGFLPAKETARRTRLAELATIDATLVMFESGSRIRRALPDLAAVLGNRAAAICRELTKLHQDIHRAGLTELAEVAATLETRGEFVLVVGPPAEDSGALSSGDLDELLLSLLKEGSVKDSVARAVEISGHPRRQVYARALELAGQIRDIGDNGCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWH2</accession>
    <name>Q3SWH2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 15 16">Protoporphyrinogen IX oxidase</fullName>
            <shortName evidence="15">PPO</shortName>
            <ecNumber evidence="15 16">1.3.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">Nwi_0101</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15 16">Catalyzes the oxidation of protoporphyrinogen IX to protoporphyrin IX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15 16">
            <text>3 A + protoporphyrinogen IX = 3 AH2 + protoporphyrin IX</text>
            <dbReference type="Rhea" id="RHEA:62000"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:57306"/>
            <dbReference type="ChEBI" id="CHEBI:57307"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 16">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="15 16">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 15 16">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 15">Cell membrane</location>
            <topology evidence="3 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 15 16">Belongs to the HemJ family.</text>
    </comment>
    <dbReference type="EC" id="1.3.99.-" evidence="15 16"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313438.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0101"/>
    <dbReference type="EnsemblBacteria" id="ABA03369">
        <property type="protein sequence ID" value="ABA03369"/>
        <property type="gene ID" value="Nwi_0101"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0101"/>
    <dbReference type="eggNOG" id="COG1981">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125006_1_0_5"/>
    <dbReference type="OMA" id="VRLFIYH"/>
    <dbReference type="OrthoDB" id="1433260at2"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00324"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070818">
        <property type="term" value="F:protoporphyrinogen oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02239">
        <property type="entry name" value="HemJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005265">
        <property type="entry name" value="HemJ-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40255">
        <property type="entry name" value="PTHR40255"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03653">
        <property type="entry name" value="UPF0093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004638">
        <property type="entry name" value="UCP004638"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00701">
        <property type="entry name" value="TIGR00701"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15 16" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 15 16" id="KW-0349">Heme</keyword>
    <keyword evidence="13 15 16" id="KW-0408">Iron</keyword>
    <keyword evidence="14 15 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 15 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="15">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="15">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR004638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ABA03369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="140" mass="16361" checksum="9E3CC453E0441016" modified="2005-10-11" version="1">MYEWIKAFHVIAIISWMAGMLYLPRLFVYHCDAEIGSKQSETFKVMERRLFKAIINPAMMIAWGLGLYMMWERQWYAVGWFQGKLGLVILMSAVHGYFARRVRDFAADRNVMSQKFYRIINEVVTALMVGIVILVIVKPS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWK7</accession>
    <name>SYFA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phenylalanine--tRNA ligase alpha subunit</fullName>
            <ecNumber evidence="1">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Phenylalanyl-tRNA synthetase alpha subunit</fullName>
            <shortName evidence="1">PheRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pheS</name>
        <name type="ordered locus">Nwi_0066</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + H(+) + L-phenylalanyl-tRNA(Phe)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium ions per tetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWK7"/>
    <dbReference type="SMR" id="Q3SWK7"/>
    <dbReference type="STRING" id="323098.Nwi_0066"/>
    <dbReference type="PRIDE" id="Q3SWK7"/>
    <dbReference type="EnsemblBacteria" id="ABA03334">
        <property type="protein sequence ID" value="ABA03334"/>
        <property type="gene ID" value="Nwi_0066"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0066"/>
    <dbReference type="eggNOG" id="COG0016">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025086_0_1_5"/>
    <dbReference type="OMA" id="DWHNFTA"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00281">
        <property type="entry name" value="Phe_tRNA_synth_alpha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004529">
        <property type="entry name" value="Phe-tRNA-synth_IIc_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004530">
        <property type="entry name" value="Phe-tRNA-synth_IIc_mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004188">
        <property type="entry name" value="Phe-tRNA_ligase_II_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022911">
        <property type="entry name" value="Phe_tRNA_ligase_alpha1_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF41">
        <property type="entry name" value="PTHR11538:SF41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02912">
        <property type="entry name" value="Phe_tRNA-synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="SSF46589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00468">
        <property type="entry name" value="pheS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000231999" description="Phenylalanine--tRNA ligase alpha subunit">
        <location>
            <begin position="1"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with beta subunit</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00281"/>
        </source>
    </evidence>
    <sequence length="369" mass="41616" checksum="4701DAA02AC45390" modified="2005-10-11" version="1">MTLCKWWRFMSDLDALQSNILADIANASDEAALEAVRVMALGKKGSISALLATLGKMSPDERKTEGAKINLAKDTVTRALAARREVLKALALDARLASETIDVTPPLRETPAEAGRIHPLSQVWDEVTTIFADMGFAVAEGPDIETDDYNFTRLNFPEGHPAREMHDTFYFNPKDDGSRLLLRTHTSPVQVRTMLSQKPPIRVICPGRTYRSDSDQTHTPMFHQVEGLVIDKSSHLGHLKWILHEFCKAFFEVDNVNMRFRPSFFPFTEPSLEVDIQCRRDKHEIRFGEGEDWLEILGCGMVHPNVLKLCGIDPEVYQGFAWGMGIDRITMLKYGIADLRQLFEGDVRWLTHYGFRPLEIPTLAGGLSS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-01-24" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWP6</accession>
    <name>RS15_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsO</name>
        <name type="ordered locus">Nwi_0027</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it helps nucleate assembly of the platform of the 30S subunit by binding and bridging several RNA helices of the 16S rRNA.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Forms an intersubunit bridge (bridge B4) with the 23S rRNA of the 50S subunit in the ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Forms a bridge to the 50S subunit in the 70S ribosome, contacting the 23S rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313366.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWP6"/>
    <dbReference type="SMR" id="Q3SWP6"/>
    <dbReference type="STRING" id="323098.Nwi_0027"/>
    <dbReference type="EnsemblBacteria" id="ABA03295">
        <property type="protein sequence ID" value="ABA03295"/>
        <property type="gene ID" value="Nwi_0027"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0027"/>
    <dbReference type="eggNOG" id="COG0184">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148518_0_0_5"/>
    <dbReference type="OMA" id="FKTHVKD"/>
    <dbReference type="OrthoDB" id="1990141at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00353">
        <property type="entry name" value="Ribosomal_S15p_S13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01343_B">
        <property type="entry name" value="Ribosomal_S15_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000589">
        <property type="entry name" value="Ribosomal_S15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005290">
        <property type="entry name" value="Ribosomal_S15_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="S15_NS1_RNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23321">
        <property type="entry name" value="PTHR23321"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00312">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01387">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="SSF47060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00952">
        <property type="entry name" value="S15_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00362">
        <property type="entry name" value="RIBOSOMAL_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000115490" description="30S ribosomal protein S15">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="89" mass="10063" checksum="A35227E0DA14AA24" modified="2005-10-11" version="1">MSIAAERKAEVIKTSANKPGDTGSPEVQVAILSERIANLTAHFKIHTKDNHSRRGLLKLVSTRRSLLDYVKKKDEARYKALLEKHGIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMW9</accession>
    <name>Q3SMW9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Arsenate reductase</fullName>
            <ecNumber evidence="3">1.20.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_3125</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>[glutaredoxin]-dithiol + arsenate + glutathione + H(+) = arsenite + glutathionyl-S-S-[glutaredoxin] + H2O</text>
            <dbReference type="Rhea" id="RHEA:22016"/>
            <dbReference type="Rhea" id="RHEA-COMP:10729"/>
            <dbReference type="Rhea" id="RHEA-COMP:17668"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29242"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:48597"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:146199"/>
            <dbReference type="EC" id="1.20.4.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 2 3">Belongs to the ArsC family.</text>
    </comment>
    <dbReference type="EC" id="1.20.4.1" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316280.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3125"/>
    <dbReference type="EnsemblBacteria" id="ABA06372">
        <property type="protein sequence ID" value="ABA06372"/>
        <property type="gene ID" value="Nwi_3125"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3125"/>
    <dbReference type="eggNOG" id="COG1393">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116644_0_0_5"/>
    <dbReference type="OMA" id="EPRIVHY"/>
    <dbReference type="OrthoDB" id="1650947at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008794">
        <property type="term" value="F:arsenate reductase (glutaredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03034">
        <property type="entry name" value="ArsC_ArsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006659">
        <property type="entry name" value="Arsenate_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006660">
        <property type="entry name" value="Arsenate_reductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30041">
        <property type="entry name" value="PTHR30041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03960">
        <property type="entry name" value="ArsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00014">
        <property type="entry name" value="arsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51353">
        <property type="entry name" value="ARSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA06372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="141" mass="15591" checksum="9EA8EC351378FDE1" modified="2005-10-11" version="1">MDVIIYHNPDCGTSRNTLALIRNAGIEPHIIEYLKTPPLRVLLAQLIARMGISARALLRDKGIPYTELGLADPKLTDDQILDAMMAHPILINRPIVVSPKGVKLCRPSEDVLDLLPPQQGELTKEDGERVIDEQGRRIATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMY6</accession>
    <name>Q3SMY6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_3108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316266.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3108"/>
    <dbReference type="EnsemblBacteria" id="ABA06355">
        <property type="protein sequence ID" value="ABA06355"/>
        <property type="gene ID" value="Nwi_3108"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3108"/>
    <dbReference type="eggNOG" id="COG5483">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2220413_0_0_5"/>
    <dbReference type="OrthoDB" id="1831995at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007438">
        <property type="entry name" value="DUF488"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04343">
        <property type="entry name" value="DUF488"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="106" mass="11868" checksum="FD9DF0325368DCB1" modified="2005-10-11" version="1">MSDYILPQDVHWPKSTILTVGHSTLPIERFVELLKIYGIEQLIDIRTMPGSRHNPQFGSDALAGSLGAAHIEYAHMGALGGLRRARKDSPNDGWRNKSFRGFADYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMZ3</accession>
    <name>Q3SMZ3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_3101</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3101"/>
    <dbReference type="EnsemblBacteria" id="ABA06348">
        <property type="protein sequence ID" value="ABA06348"/>
        <property type="gene ID" value="Nwi_3101"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3101"/>
    <dbReference type="eggNOG" id="ENOG5033CBD">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165561_0_0_5"/>
    <dbReference type="OMA" id="RVGAFIH"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="90" mass="10259" checksum="485F9F118714FA4F" modified="2005-10-11" version="1">MENVMTVRSRTDSVVFKHSFCIEGIDRPFPPGTYEVVTDEEEIEGLSFLAFRRIATSFRATDSRRARLTTESFDIDPDDLKEALRIDAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN63</accession>
    <name>Q3SN63_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">OmpA/MotB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_3028</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3028"/>
    <dbReference type="EnsemblBacteria" id="ABA06278">
        <property type="protein sequence ID" value="ABA06278"/>
        <property type="gene ID" value="Nwi_3028"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3028"/>
    <dbReference type="eggNOG" id="COG2885">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016890_5_2_5"/>
    <dbReference type="OMA" id="IERYRIQ"/>
    <dbReference type="OrthoDB" id="2018797at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07185">
        <property type="entry name" value="OmpA_C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006664">
        <property type="entry name" value="OMP_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006665">
        <property type="entry name" value="OmpA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006690">
        <property type="entry name" value="OMPA-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036737">
        <property type="entry name" value="OmpA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00691">
        <property type="entry name" value="OmpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01021">
        <property type="entry name" value="OMPADOMAIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103088">
        <property type="entry name" value="SSF103088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01068">
        <property type="entry name" value="OMPA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51123">
        <property type="entry name" value="OMPA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="1 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229163" evidence="4">
        <location>
            <begin position="25"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="OmpA-like" evidence="5">
        <location>
            <begin position="88"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA06278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="213" mass="22694" checksum="0B2121819AEA1715" modified="2005-10-11" version="1">MLIQRISGKSLGAAILAASLMAIAAPQGVSADDLSAQQIIDGLKVSKTRSLSASPRPTLDPNDLNFVQKVRGKTRSLSMGDRSQLAEIAAKRPKIDLDINFDFDSAAVTPRTEPQLHSLGKALTSDELAGSVIMLGGHTDGKGSDAYNQNLSERRAEAVKRFLVETYKIPAANLVAAGYGKEGYKNPADPFAAENRRVEVVNMATKDQAANSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN68</accession>
    <name>Q3SN68_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sulfite dehydrogenase (Cytochrome) subunit SorB</fullName>
            <ecNumber evidence="2">1.8.2.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_3023</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.8.2.1" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316191.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3023"/>
    <dbReference type="EnsemblBacteria" id="ABA06273">
        <property type="protein sequence ID" value="ABA06273"/>
        <property type="gene ID" value="Nwi_3023"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3023"/>
    <dbReference type="eggNOG" id="COG2857">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155036_0_0_5"/>
    <dbReference type="OMA" id="MICHAAQ"/>
    <dbReference type="OrthoDB" id="1939800at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050310">
        <property type="term" value="F:sulfite dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229048" evidence="1">
        <location>
            <begin position="30"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="110" mass="11704" checksum="A157307B5E1B0F6B" modified="2005-10-11" version="1">MTRSVALALATGIGLAASLYTCASTTATAGPLSYELPRETIEFKPGPNLDVVKNNCLACHSADYIATQPRGPKFGKDFWQAEVNKMIGAYGAQIDEADIGKIVDYLAATY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNA7</accession>
    <name>Q3SNA7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2984</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316156.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2984"/>
    <dbReference type="EnsemblBacteria" id="ABA06234">
        <property type="protein sequence ID" value="ABA06234"/>
        <property type="gene ID" value="Nwi_2984"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2984"/>
    <dbReference type="eggNOG" id="ENOG5033I2W">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_193733_0_0_5"/>
    <dbReference type="OMA" id="NRHKAGV"/>
    <dbReference type="OrthoDB" id="2068659at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="68" mass="7751" checksum="3EB1052DF8DAD01E" modified="2005-10-11" version="1">MTRSNFWVIGGEFGSMNFHKLVEGSAQVQGPFKTRQEAEDAWKVVSEENRYRAGVRFSIVEEPNRMAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNB9</accession>
    <name>Q3SNB9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2972</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2972"/>
    <dbReference type="EnsemblBacteria" id="ABA06222">
        <property type="protein sequence ID" value="ABA06222"/>
        <property type="gene ID" value="Nwi_2972"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2972"/>
    <dbReference type="eggNOG" id="COG2223">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040537_2_0_5"/>
    <dbReference type="OMA" id="YFYMASF"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="104"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="193"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="237"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="308"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="365"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="395"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="40"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA06222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="422" mass="45170" checksum="7BAA482455FED780" modified="2005-10-11" version="1">MQQSEKLPVDKPVISEETLVAPVVIPGITEKSATGPAYLVLGGISFSHFLNDTMQSLIPSVYPILKENYALDFAQIGMITLAFQVTASLLQPVVGYVTDSKPQPFSLAVGMGFTFVGLLLLSGAHSYAMILVAAALIGLGSAVFHPESARIARMASGGRYGFAQSVFQVGGNFGAAMGPLLAALIIVPFGQPSIAWFSSIAFVAIVLLVQIGRWYRPRIVPRKSAAQIRSPEAPTRARTMVALAVLIVLVFSKFIYMSSLTSYYTFYLIEKFHVSTQTSQLLLFVFLGAVAIGVYFGGPIGDRFGRRYVIWLSILGVLPFTLLLPFANLAGTVALTVVIGVILSSAMPAIIVFAQELMPHRLGMISGMFFGFAFGAAGLGAALLGEVADAKGIEFVYRVCAFLPAIGLLAMFLPKMPKNQHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNG1</accession>
    <name>Q3SNG1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulatory protein, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_2930</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NodD regulates the expression of the nodABCFE genes which encode other nodulation proteins. NodD is also a negative regulator of its own expression. Binds flavonoids as inducers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2930"/>
    <dbReference type="EnsemblBacteria" id="ABA06180">
        <property type="protein sequence ID" value="ABA06180"/>
        <property type="gene ID" value="Nwi_2930"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2930"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_1_5"/>
    <dbReference type="OMA" id="GATACIH"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0536">Nodulation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="5">
        <location>
            <begin position="7"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA06180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="320" mass="35771" checksum="66257B81F1F90532" modified="2005-10-11" version="1">MHQFMHLTLRQLQIFDAVVRRRNFTRAADALHLTQPAVSMQMRQLEEQIGVPLFDYVGKQIQPTDAGRELHRHATLIGAKMRDLTSAMEEFRGVHRGELDLIVASTANYFMPKLIAAFCASHAKVQVRLQVSNQTHILESLSASNQDLAIMGQPPEDAELVARGFLDNPLMVIAPPAHPLARRRKIPLTELGCQPFITREPDSGTRHAAERHFSRHGVEFHSVMEMSSSEAIKQAVEAGLGLGVLSLHTLELELATRRLVTLRVESFPIVRQWFVVHRKGKRLSAVAQSFLAFLTKQARPLASPKQSPSHSGLQAFGLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNL3</accession>
    <name>Q3SNL3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2878</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2878"/>
    <dbReference type="EnsemblBacteria" id="ABA06128">
        <property type="protein sequence ID" value="ABA06128"/>
        <property type="gene ID" value="Nwi_2878"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2878"/>
    <dbReference type="HOGENOM" id="CLU_2437845_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="90" mass="10466" checksum="D0695DD1C08D3682" modified="2005-10-11" version="1">MTGVGRRPEEPTRILNMQQSSNVRARSEQAFRDSIVMSPEHAASFKDAHRYRLASEAPASFARLRMHEYLRAPRKWLASINGRVRKRENE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNL4</accession>
    <name>Q3SNL4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2877</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316059.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2877"/>
    <dbReference type="EnsemblBacteria" id="ABA06127">
        <property type="protein sequence ID" value="ABA06127"/>
        <property type="gene ID" value="Nwi_2877"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2877"/>
    <dbReference type="eggNOG" id="COG0189">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055286_0_1_5"/>
    <dbReference type="OMA" id="FLECNQS"/>
    <dbReference type="OrthoDB" id="937913at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="319" mass="36031" checksum="37839A17FE7412AE" modified="2005-10-11" version="1">MPGNVDVLILSSLADISTDRICYHLSTMGIGFLRLNREQLRDVGLQLDPARARLTCRYVDQVWQVGTNLRSVWWRQPTFLRNTPGTALTVEEQLDRSQWSAAMRGLMLFSEARWFNNPAATYRAESKPFQLREAYKLGFDVPETLVTNDRFADVPRIVGQKLAVKSIDTVLLADHDNQHFGYTIVTDWSDCADESFHAVPVACQALLSPKLDLRVTIIGEHVWCTTVEVEGGGIEGDWRLTPKTDLIYREHELPIEVQRRCLALIRHLGLGYGAIDLALSAGRYWFIEINPTGEWGWLDRDGRGISEAIAMELASEGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNT9</accession>
    <name>Q3SNT9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Succinate dehydrogenase hydrophobic membrane anchor subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">Nwi_2799</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Membrane-anchoring subunit of succinate dehydrogenase (SDH).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Carbohydrate metabolism; tricarboxylic acid cycle.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur protein, plus two membrane-anchoring proteins, SdhC and SdhD.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315997.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2799"/>
    <dbReference type="EnsemblBacteria" id="ABA06052">
        <property type="protein sequence ID" value="ABA06052"/>
        <property type="gene ID" value="Nwi_2799"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2799"/>
    <dbReference type="eggNOG" id="COG2142">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151315_0_0_5"/>
    <dbReference type="OMA" id="MKLGMQV"/>
    <dbReference type="OrthoDB" id="2048140at2"/>
    <dbReference type="UniPathway" id="UPA00223"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000104">
        <property type="term" value="F:succinate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014312">
        <property type="entry name" value="Succ_DH_anchor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000701">
        <property type="entry name" value="SuccDH_FuR_B_TM-su"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01127">
        <property type="entry name" value="Sdh_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81343">
        <property type="entry name" value="SSF81343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02968">
        <property type="entry name" value="succ_dehyd_anc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0349">Heme</keyword>
    <keyword evidence="13" id="KW-0408">Iron</keyword>
    <keyword evidence="14 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00019425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ABA06052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="135" mass="14565" checksum="212A252032702F54" modified="2005-10-11" version="1">MTATPPSGAKSMRTPLGRVRNHGSAHSGTTDFWRQRLTSVAMILLIVPMVVVMMILLGRNQAGAAQILGSPPIAIIMLLFVVASIWHMKIGMQVIIEDYVHDGKLKLASIMANNFFSVTLALTAAYAIFKLSSGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNV4</accession>
    <name>Q3SNV4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2784</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2784"/>
    <dbReference type="EnsemblBacteria" id="ABA06037">
        <property type="protein sequence ID" value="ABA06037"/>
        <property type="gene ID" value="Nwi_2784"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2784"/>
    <dbReference type="HOGENOM" id="CLU_2524188_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="84" mass="9550" checksum="D079D5C7284DAFC9" modified="2005-10-11" version="1">MLYKILPGRIFLRRTGLHFAGKCPIYPERRRSFDCRGGCGHGIKDAMITDRISSDIGRFTARIVIDDRSRLPGRFFGRFATSAT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP21</accession>
    <name>TOLB_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tol-Pal system protein TolB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tolB</name>
        <name type="ordered locus">Nwi_2717</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the Tol-Pal system, which plays a role in outer membrane invagination during cell division and is important for maintaining outer membrane integrity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The Tol-Pal system is composed of five core proteins: the inner membrane proteins TolA, TolQ and TolR, the periplasmic protein TolB and the outer membrane protein Pal. They form a network linking the inner and outer membranes and the peptidoglycan layer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TolB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SP21"/>
    <dbReference type="SMR" id="Q3SP21"/>
    <dbReference type="STRING" id="323098.Nwi_2717"/>
    <dbReference type="EnsemblBacteria" id="ABA05970">
        <property type="protein sequence ID" value="ABA05970"/>
        <property type="gene ID" value="Nwi_2717"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2717"/>
    <dbReference type="eggNOG" id="COG0823">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047123_0_0_5"/>
    <dbReference type="OMA" id="VREPSWG"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017038">
        <property type="term" value="P:protein import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00671">
        <property type="entry name" value="TolB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011659">
        <property type="entry name" value="PD40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014167">
        <property type="entry name" value="Tol-Pal_TolB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007195">
        <property type="entry name" value="TolB_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07676">
        <property type="entry name" value="PD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04052">
        <property type="entry name" value="TolB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02800">
        <property type="entry name" value="propeller_TolB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0574">Periplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000259060" description="Tol-Pal system protein TolB" evidence="1">
        <location>
            <begin position="38"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00671"/>
        </source>
    </evidence>
    <sequence length="450" mass="49097" checksum="C1FC61D89E6EEA15" modified="2005-10-11" version="1" precursor="true">MIERNGLQRMPFRLNRRHMISGMASAAVLLGSRQALGQARVQITEGNVAPLPIAIPNFVGGTPSDNEVGAGVAKVITNNLKRSGLFAPIDQAAYIEKITNIDVPPQFANWKTINAQALVTGRMTRQGDGRLKAEFRLWDVATGQQLAGQQYFTSPEYWRRIAHIISDQIYERLTGEKGYFDSRVVFVDESGSSQRRVKRLALMDQDGANVRYLTRGADLVITPRFSPSTQEVTYMEFGQGDPRVYLFNIETGQREIVGNFPGMSFSPRFAPDGQRIIMSLQQGGNSNLFVMDLRSKSTTRLTDTPAIDTSPSYSPDGARICFESDRGGKPQIYVMGATGGGAQRISFGEGSYSTPVWSPRGDYIAFTKQGGGQFSIGIIKPDGSGERILTSGFHNEGPTFAPNGRVLMFFRDPGGGGGPSLYTIDVSGRNELKVPTPGFASDPAWSPLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPA1</accession>
    <name>Q3SPA1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2637</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05890">
        <property type="protein sequence ID" value="ABA05890"/>
        <property type="gene ID" value="Nwi_2637"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2637"/>
    <dbReference type="HOGENOM" id="CLU_2451640_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="89" mass="9658" checksum="C9DDB7FC201121FC" modified="2005-10-11" version="1">MASMLTVRPGSNVAYAIASGGGLSDAPMAAIAIRGDQPRMIARFDQRMVETSTVRFEANLRLARGGSFTDPHALPRSCYECPRSMRRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPA9</accession>
    <name>Q3SPA9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2629</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315832.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2629"/>
    <dbReference type="EnsemblBacteria" id="ABA05882">
        <property type="protein sequence ID" value="ABA05882"/>
        <property type="gene ID" value="Nwi_2629"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2629"/>
    <dbReference type="eggNOG" id="COG5426">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_400015_0_0_5"/>
    <dbReference type="OMA" id="WLMKEPE"/>
    <dbReference type="OrthoDB" id="102505at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="660"/>
            <end position="683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="687" mass="74126" checksum="82673D61633D8B9D" modified="2005-10-11" version="1">MQYGIAFAPLVPTIVLWSALAAIVVITVLLLVGRARGAAVRVAALALILLALANPSFTREEREPLASVAAVIVDKSPSQNFGDRTDQAAKARDALVDRLKQVKGLEVRIVDAGQADGETDGTRLFSALSSTLSDVPTDRVAAAFLITDGRVHDIPDNVAALGFKAPVHALITGHTNERDRRVAIVAAPRFGIVGQSQTVTYRLDDQGVSGELAKVVVRRDGDVISERSVLSGETVKVDVGIKHAGQNIIEIEASPLANELTLVNNRAVVPIDGVRDKLRVLLVSGEPHSGERTWRNLLKSDPSVDLVHFTILRPPEKQDGTPIHELSLIAFPTRELFQEKINEFQLIIFDRYARQGVLPTAYFDSIARYVRAGGAVLVSAGPDYASTTSIWRTPLDAVLPAEPVGVTEKPFHARLSEAGKRHPVTRGLEGSASDPPRWSRFFREVDTRNVAVPPVMTGADGQPLLLLSRYDEGRVALLLSDHIWLWARGFEGGGPHLDLLRRMSHWLMKQPELEEETLRLAVQGKDLVVQRQTMADTVAPVTVTSPSGKTRELTLEAAEPGLWKAALAADELGLWQATDGTLKALINVGPANPKEFSEVTSTVGGLRPIAQASGGDARRIADGSAINIPRIVPVHASSVFRGEGWLGVRMRDASVVRGVGVLPVFTGLIGLLLLLGAFAATWLREGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPB2</accession>
    <name>Q3SPB2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATPase associated with various cellular activities, AAA_3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2626</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315829.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2626"/>
    <dbReference type="EnsemblBacteria" id="ABA05879">
        <property type="protein sequence ID" value="ABA05879"/>
        <property type="gene ID" value="Nwi_2626"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2626"/>
    <dbReference type="eggNOG" id="COG0714">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034716_3_1_5"/>
    <dbReference type="OMA" id="SRAWAWL"/>
    <dbReference type="OrthoDB" id="688156at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011703">
        <property type="entry name" value="ATPase_AAA-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041628">
        <property type="entry name" value="ChlI/MoxR_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07726">
        <property type="entry name" value="AAA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17863">
        <property type="entry name" value="AAA_lid_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_3" evidence="1">
        <location>
            <begin position="58"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="AAA_lid_2" evidence="2">
        <location>
            <begin position="267"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="337" mass="36313" checksum="D4348DDFFEB882F2" modified="2005-10-11" version="1">MAAADPDKGVEKLEDVIVRSAEQVAEQIRAARDAISNVIFGQDRVIENTLVTILSGGHALLIGVPGLAKTKLVETLGVTLGLDAKRVQFTPDLMPSDILGAEVLDESSIGKRSFRFIPGPVFAQLLMADEINRASPRTQSALLQAMQEQHITVAGVRHDLPKPFHVLATQNPLEQEGTYPLPEAQLDRFLMEIDVGYPDRDAERRILFETTGAGEALAKAAMNADAVLAAQRLVRRLPVGDSVVEAILSLVRSARPGPESGETGKLIAWGPGPRASQSLMLAVRARALLDGRLAPSIDDVLDLAEPVLKHRMALTFSARAEGRSIADVIRQLKSRIG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPI3</accession>
    <name>LPXK_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tetraacyldisaccharide 4'-kinase</fullName>
            <ecNumber evidence="1">2.7.1.130</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Lipid A 4'-kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lpxK</name>
        <name type="ordered locus">Nwi_2555</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + {2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-beta-D-glucosaminyl}-(1-&gt;6)-{2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-alpha-D-glucosaminyl phosphate} = ADP + {2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-4-O-phospho-beta-D-glucosaminyl}-(1-&gt;6)-{2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-alpha-D-glucosaminyl phosphate}.</text>
            <dbReference type="EC" id="2.7.1.130"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 6/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LpxK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.130" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315759.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SPI3"/>
    <dbReference type="SMR" id="Q3SPI3"/>
    <dbReference type="STRING" id="323098.Nwi_2555"/>
    <dbReference type="EnsemblBacteria" id="ABA05808">
        <property type="protein sequence ID" value="ABA05808"/>
        <property type="gene ID" value="Nwi_2555"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2555"/>
    <dbReference type="eggNOG" id="COG1663">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038816_0_0_5"/>
    <dbReference type="OMA" id="MDDGFQN"/>
    <dbReference type="OrthoDB" id="1382484at2"/>
    <dbReference type="UniPathway" id="UPA00359">
        <property type="reaction ID" value="UER00482"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009029">
        <property type="term" value="F:tetraacyldisaccharide 4'-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009245">
        <property type="term" value="P:lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00409">
        <property type="entry name" value="LpxK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003758">
        <property type="entry name" value="LpxK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42724">
        <property type="entry name" value="PTHR42724"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02606">
        <property type="entry name" value="LpxK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00682">
        <property type="entry name" value="lpxK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0441">Lipid A biosynthesis</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000229966" description="Tetraacyldisaccharide 4'-kinase">
        <location>
            <begin position="1"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="51"/>
            <end position="58"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00409"/>
        </source>
    </evidence>
    <sequence length="337" mass="35569" checksum="CD0AF94F658A03D5" modified="2005-10-11" version="1">MREPAFWRRPSSLLSRLLIPVGALYGAIAARRLSRTGLRAGVPVICVGNYHLGGAGKTPTVLALAGILRSLGETPVVISRGYGGRLRGPVRVDPDRHAAADVGDEPLMMARTLPVIVSRQRAAGVAPARALGASVILMDDGFQNPTLARDISLIVIDGDRGLGNRRIFPAGPLRAPLPPQLARTDALVIVGPGSAADDIAASIEARGGPVLRARVVPDEASVAALRGRRVLAFAGIGDPSRFFRGLRACGVDVAAERAFADHHPFSQRDVAALQSAAEKDGLTLVTTEKDLARLRNNENIAAFAQAVAPFAVTLAFDDETALRSFLMDGIAKVRRLD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-12-20" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPJ6</accession>
    <name>SYI_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Isoleucine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Isoleucyl-tRNA synthetase</fullName>
            <shortName evidence="1">IleRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ileS</name>
        <name type="ordered locus">Nwi_2542</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile)</text>
            <dbReference type="Rhea" id="RHEA:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:9666"/>
            <dbReference type="Rhea" id="RHEA-COMP:9695"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78528"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.5" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315746.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SPJ6"/>
    <dbReference type="SMR" id="Q3SPJ6"/>
    <dbReference type="STRING" id="323098.Nwi_2542"/>
    <dbReference type="PRIDE" id="Q3SPJ6"/>
    <dbReference type="EnsemblBacteria" id="ABA05795">
        <property type="protein sequence ID" value="ABA05795"/>
        <property type="gene ID" value="Nwi_2542"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2542"/>
    <dbReference type="eggNOG" id="COG0060">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001493_7_1_5"/>
    <dbReference type="OMA" id="HLGTAWN"/>
    <dbReference type="OrthoDB" id="32262at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004822">
        <property type="term" value="F:isoleucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006428">
        <property type="term" value="P:isoleucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07960">
        <property type="entry name" value="Anticodon_Ia_Ile_BEm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02002">
        <property type="entry name" value="Ile_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033708">
        <property type="entry name" value="Anticodon_Ile_BEm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002301">
        <property type="entry name" value="Ile-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023585">
        <property type="entry name" value="Ile-tRNA-ligase_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00984">
        <property type="entry name" value="TRNASYNTHILE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="SSF50677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00392">
        <property type="entry name" value="ileS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000098430" description="Isoleucine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="67"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="668"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="627"/>
        </location>
        <ligand>
            <name>L-isoleucyl-5'-AMP</name>
            <dbReference type="ChEBI" id="CHEBI:178002"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="671"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02002"/>
        </source>
    </evidence>
    <sequence length="999" mass="111640" checksum="689891A0DB8D55C8" modified="2005-10-11" version="1">MTEKKKNSDAPDYAKTLFLPQTEFPMRAGLPQREPDILKRWTDIDLYGQLRACAKGRPKFVLHDGPPYANGSIHIGHALNKILKDVVTKSQQMLGHDSNYVPGWDCHGLPIEWKIEEENYRSKGKTKPNFKDPAAMVAFRKECRAYAEHWLNVQREQFKRLGIIGDWDHPYATMTYPAEAQIARELMKFAANGTLYRGSKPVMWSVVEKTALAEAEVEYEDHTSDTVWVKFPVTSPAHGALSQASVVIWTTTPWTLPGNRAISFSNKIAYGLYKITDAPADNWAKTGDVLILANKLAGEVFKQARVTAYEKVRDIPADTLDAVECAHPLKGLSGGYEFTVPLLDGDHVTDDTGTGFVHTAPGHGREDFDIWTANARELEVRGISSTIPYTVDENGALTAQAPGFEGKRVIDDKGNKGDANEAVIKALVERNRLLARGRLKHQYPHSWRSKKPVIFRNTPQWFIAMDRDIADDGKANHGDTLRARALQAISVTRWVPPAGQNRITGMIANRPDWVISRQRAWGVPIAVFVREKGDGSAEVLIDDAVNRRISDAFEKEGADAWYAEGARERFLGDRAGEDWSKVDDILDVWFDSGSTHAFVLEDPAHFPSLRDIKRKADGGRDTVMYLEGSDQHRGWFHSSLLESCGTRGRAPYDIVLTHGFTLDENGRKMSKSLGNTVDPQKVIKDSGADILRLWVCATDYADDQRIGPEILKNTIETYRKLRNTIRWMLGTLHHFDRGEAVAFTEMPELERLMLHRLAEQSALVRSAYAEFDYKTVVASLAAFMNTELSAFYFDIRKDTLYCDPPSSPARKAALTVIDKLCDAILKWLAPVLSFTTEEAWRLYRPDAEPSVHLTLFPESFDNFRDDSLAAKWETVRAVRSVVTGALELERAAKRIGSSLEASPIVHVADRAMLGTLFDIDLAEVCITSNFEVREGDAPDGAFRLPNVQGVAVVVEKAAGTKCARSWKILPTVGSDSEYPDVSPRDAQALREWKALGVTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPK4</accession>
    <name>Q3SPK4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF559 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2534</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315738.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05787">
        <property type="protein sequence ID" value="ABA05787"/>
        <property type="gene ID" value="Nwi_2534"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2534"/>
    <dbReference type="eggNOG" id="COG2852">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107928_4_0_5"/>
    <dbReference type="OMA" id="KLRSDQT"/>
    <dbReference type="OrthoDB" id="2013215at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007569">
        <property type="entry name" value="DUF559"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04480">
        <property type="entry name" value="DUF559"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF559" evidence="1">
        <location>
            <begin position="9"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="114" mass="13062" checksum="6D2FFE22C6393EE0" modified="2005-10-11" version="1">MRGPKPNVTGFARHLRISQTQAEAKLWSRIRARQIDGRKFVRQVPVGPYFCDFVCREQKLVVEVDGGQHADSQGDHIRDRYLTMNGYRVLRFWNNDVLGNIEGVVSVIHAELIG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPM6</accession>
    <name>RL25_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L25</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">General stress protein CTC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplY</name>
        <name evidence="1" type="synonym">ctc</name>
        <name type="ordered locus">Nwi_2512</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This is one of the proteins that binds to the 5S RNA in the ribosome where it forms part of the central protuberance.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit; part of the 5S rRNA/L5/L18/L25 subcomplex. Contacts the 5S rRNA. Binds to the 5S rRNA independently of L5 and L18.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL25 family. CTC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315716.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SPM6"/>
    <dbReference type="SMR" id="Q3SPM6"/>
    <dbReference type="STRING" id="323098.Nwi_2512"/>
    <dbReference type="EnsemblBacteria" id="ABA05765">
        <property type="protein sequence ID" value="ABA05765"/>
        <property type="gene ID" value="Nwi_2512"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2512"/>
    <dbReference type="eggNOG" id="COG1825">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075939_0_0_5"/>
    <dbReference type="OMA" id="HVDFYEV"/>
    <dbReference type="OrthoDB" id="1673077at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00495">
        <property type="entry name" value="Ribosomal_L25_TL5_CTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01334">
        <property type="entry name" value="Ribosomal_L25_CTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_L25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029751">
        <property type="entry name" value="Ribosomal_L25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_L25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020057">
        <property type="entry name" value="Ribosomal_L25_b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037121">
        <property type="entry name" value="Ribosomal_L25_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001021">
        <property type="entry name" value="Ribosomal_L25_long"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01386">
        <property type="entry name" value="Ribosomal_L25p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14693">
        <property type="entry name" value="Ribosomal_TL5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="SSF50715"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00731">
        <property type="entry name" value="bL25_bact_ctc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000244218" description="50S ribosomal protein L25">
        <location>
            <begin position="1"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="228" mass="23822" checksum="EAD01458AA3FF17A" modified="2005-10-11" version="1">MATVMELKATARPKSGKGAARAERRAGRVPAVIYGNKQPPQPISVEEPELRQRILAGRFLTTIFDINLDGKKHRVIPRDFHLDPVKDFPIHVDFMRLGEGATIRVSVPLRLLKADVAPGVKRGGTVNLVTHAIDVECAAESIPQFIEADVGALEISHSLHLSDLQLPPGVKPLAREDVTLVTIVPPSGYAEELKAAAAAGAAAPAAGAAPAAAAAGAKAPAGGGDKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPR3</accession>
    <name>Q3SPR3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">Bacterioferritin</fullName>
            <ecNumber evidence="4">1.16.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_2475</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Iron-storage protein, whose ferroxidase center binds Fe(2+) ions, oxidizes them by dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>4 Fe(2+) + 4 H(+) + O2 = 4 Fe(3+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11148"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.16.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4 6">Belongs to the bacterioferritin family.</text>
    </comment>
    <dbReference type="EC" id="1.16.3.1" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315681.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2475"/>
    <dbReference type="EnsemblBacteria" id="ABA05728">
        <property type="protein sequence ID" value="ABA05728"/>
        <property type="gene ID" value="Nwi_2475"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2475"/>
    <dbReference type="eggNOG" id="COG2193">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104506_2_0_5"/>
    <dbReference type="OMA" id="YQRLFHV"/>
    <dbReference type="OrthoDB" id="1890523at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:cellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00907">
        <property type="entry name" value="Bacterioferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002024">
        <property type="entry name" value="Bacterioferritin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008331">
        <property type="entry name" value="Ferritin_DPS_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00210">
        <property type="entry name" value="Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002560">
        <property type="entry name" value="Bacterioferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00601">
        <property type="entry name" value="BACFERRITIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00754">
        <property type="entry name" value="bfr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00549">
        <property type="entry name" value="BACTERIOFERRITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="4 6" id="KW-0409">Iron storage</keyword>
    <keyword evidence="4 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="7">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR002560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR002560-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA05728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="160" mass="18685" checksum="757F06F4A7BB6ED8" modified="2005-10-11" version="1">MKGDPKVIDYLNKALRHELTAVNQYWLHYRLLDNWGIKDLAKKWRAESIEEMEHADKLIDRIIFLDGFPNMQVLDPLHIGQNVGEVMDCDLKAEYSARALYEEAATHCHAVKDYVTRDIFESLMKDEESHIDFLETQIDLIKKIGVELYSQKHIGEMDHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPS8</accession>
    <name>Q3SPS8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2460</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315668.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05713">
        <property type="protein sequence ID" value="ABA05713"/>
        <property type="gene ID" value="Nwi_2460"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2460"/>
    <dbReference type="eggNOG" id="COG3360">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161196_0_1_5"/>
    <dbReference type="OMA" id="FRVNVKI"/>
    <dbReference type="OrthoDB" id="2025589at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009923">
        <property type="entry name" value="Dodecin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025543">
        <property type="entry name" value="Dodecin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036694">
        <property type="entry name" value="Dodecin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39324">
        <property type="entry name" value="PTHR39324"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07311">
        <property type="entry name" value="Dodecin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89807">
        <property type="entry name" value="SSF89807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="70" mass="7878" checksum="335168E86D1A5752" modified="2005-10-11" version="1">MAESVYKVIELIGTSKDSWEKAAKGAVERAGESLRDLRVAEIVKLDMQLDEEGKVETYRAKLKVSFKFEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPX0</accession>
    <name>Q3SPX0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cyclic nucleotide-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2417</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315629.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2417"/>
    <dbReference type="EnsemblBacteria" id="ABA05671">
        <property type="protein sequence ID" value="ABA05671"/>
        <property type="gene ID" value="Nwi_2417"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2417"/>
    <dbReference type="eggNOG" id="COG0664">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075053_3_1_5"/>
    <dbReference type="OMA" id="GERTCFA"/>
    <dbReference type="OrthoDB" id="1596937at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012318">
        <property type="entry name" value="HTH_CRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13545">
        <property type="entry name" value="HTH_Crp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00419">
        <property type="entry name" value="HTH_CRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="SSF51206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51063">
        <property type="entry name" value="HTH_CRP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="17"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="HTH crp-type" evidence="3">
        <location>
            <begin position="151"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="250" mass="27387" checksum="5449D9DDA3463B01" modified="2005-10-11" version="1">MSKIPPLSRQALSNLELFRGLPATALDEVLASAAPRSFARDCRVFNQGETRVRAHVLVDGWIRISQSGSDGGQVVLRFIGPGDTFGAVSLFTDRRYPADADTLTDATEVSWSDKALLDLMNSHPQIAINAIRIVGQRLQEAQHRVRELATQSAERRVAHALLRLTRQVGTATARGIVIRFPLRRKDIADIAGTTLYSASRILSAWERAGWLKTLDRRIAVESLSELVRIAEDESETGRGAIAASADRHAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ31</accession>
    <name>Q3SQ31_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein related to arylsulfate sulfotransferase involved in siderophore biosynthesis</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2356</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2356"/>
    <dbReference type="EnsemblBacteria" id="ABA05610">
        <property type="protein sequence ID" value="ABA05610"/>
        <property type="gene ID" value="Nwi_2356"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2356"/>
    <dbReference type="eggNOG" id="COG4321">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1756930_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3990.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027373">
        <property type="entry name" value="RHH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038268">
        <property type="entry name" value="RHH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13467">
        <property type="entry name" value="RHH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RHH_4" evidence="2">
        <location>
            <begin position="22"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="148" mass="16222" checksum="378152C8503B68CD" modified="2005-10-11" version="1">MHNDESGDRRGEPELLREYAVVKRSVVVGGHKTSVSLEDAFWTSLKDIATRQGITLSTQIASIDTNRRTSNLSSAIRLYVLEHFRMRATSTCPPSGLLRQFRSFREGRISGSSQPIWSADETEIRAGESTGRAGREGHPPGNAPAVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ35</accession>
    <name>Q3SQ35_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2352</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315570.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2352"/>
    <dbReference type="EnsemblBacteria" id="ABA05606">
        <property type="protein sequence ID" value="ABA05606"/>
        <property type="gene ID" value="Nwi_2352"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2352"/>
    <dbReference type="eggNOG" id="COG3814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106715_0_0_5"/>
    <dbReference type="OMA" id="QMTIVIQ"/>
    <dbReference type="OrthoDB" id="1620073at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007481">
        <property type="entry name" value="SspB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036760">
        <property type="entry name" value="SspB-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04386">
        <property type="entry name" value="SspB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101738">
        <property type="entry name" value="SSF101738"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="182" mass="20081" checksum="CDB476A0BC169DAA" modified="2005-10-11" version="1">MPTDHIRYDLLTSDAMRGVLRRVLTDAAERGLPGEHHFFITFKSKADGVTISPRLLTQYPEEMTIILQHQFWDLIVTEDHFEVGLSFGGVPERLVVPFSAIKSFFDPSVQFGLQFEPAETGEAQAEKAETEETSTSEPSTAPSPTALAVPADPSQPAAAESENKPKPGQGAEVVRLDRFRKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-28" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ49</accession>
    <name>LEU1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">2-isopropylmalate synthase</fullName>
            <ecNumber evidence="1">2.3.3.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Alpha-IPM synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Alpha-isopropylmalate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">leuA</name>
        <name type="ordered locus">Nwi_2338</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3-methyl-2-oxobutanoate + acetyl-CoA + H2O = (2S)-2-isopropylmalate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21524"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.13" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315556.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SQ49"/>
    <dbReference type="SMR" id="Q3SQ49"/>
    <dbReference type="STRING" id="323098.Nwi_2338"/>
    <dbReference type="EnsemblBacteria" id="ABA05592">
        <property type="protein sequence ID" value="ABA05592"/>
        <property type="gene ID" value="Nwi_2338"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2338"/>
    <dbReference type="eggNOG" id="COG0119">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022158_0_1_5"/>
    <dbReference type="OMA" id="NTMRMLV"/>
    <dbReference type="OrthoDB" id="840579at2"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00070"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003852">
        <property type="term" value="F:2-isopropylmalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01025">
        <property type="entry name" value="LeuA_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013709">
        <property type="entry name" value="2-isopropylmalate_synth_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036230">
        <property type="entry name" value="LeuA_allosteric_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005671">
        <property type="entry name" value="LeuA_bact_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08502">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00917">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110921">
        <property type="entry name" value="SSF110921"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00973">
        <property type="entry name" value="leuA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0432">Leucine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000149232" description="2-isopropylmalate synthase">
        <location>
            <begin position="1"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="2">
        <location>
            <begin position="12"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01151"/>
        </source>
    </evidence>
    <sequence length="519" mass="56216" checksum="4D226491C4AA5285" modified="2005-10-11" version="1">MTPANKSDKDRVVIFDTTLRDGEQCPGATMTFEEKIEVAEMLDGMGVDIIEAGFPIASVGDFEAVAEIARNARNAVIAGLARAIPGDIARAGEAVRHARRGRIHTFVSTSPIHLAHQMRKSEAEVLEIITATVKQARNLVEDVEWSAMDATRTPIDYLCKCVEAAIAAGATTINLPDTVGYAVPDEYRRMFKTIRERVPSADKAIFSVHCHDDLGLAVANSLAGVEGGARQVESTINGIGERAGNAALEEVVMAIKTRADVMPYWCNVESTMLTRASKMVSAATSFPVQYNKAIVGRNAFAHESGIHQDGMLKNAQTYEIMTPESVGVKQTSLVMGKHSGRHAFQHKLEELGYKLAENQLQDAFVRFKALADRKKDIYDEDIIALVDEEMAATHDRIKLSSLTVIAGTHGPQRATMKLTVSGQTRIEEAEGNGPVDAVFNCIKRLVPHEAKLELYQVHAVTQGTDAQAEVSVRLSQDGRAMTSKAADPDTLVASAKAYLGALNKIVMKHQRDVPAAAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ95</accession>
    <name>Q3SQ95_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2292</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05546">
        <property type="protein sequence ID" value="ABA05546"/>
        <property type="gene ID" value="Nwi_2292"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2292"/>
    <dbReference type="eggNOG" id="ENOG502ZXRJ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1641962_0_0_5"/>
    <dbReference type="OMA" id="DGCVGRW"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="161" mass="16509" checksum="37EC94EEAE555D5D" modified="2005-10-11" version="1">MIERRAVQGRETGMLRDFQVALMAAIALATATSVAIAGLSQDRREDQPPRSRPKPPHARQSNPPAQGNLPAFGGFDGGWSFTGTSTNCQGSGGGSFTVSGTRVVVSGGGSGRVSRSGAFQASTNAGGGVRLSAVGRLSGNRGGGSYQRSDGCVGRWTAIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQK9</accession>
    <name>Q3SQK9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha/beta hydrolase fold protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2178</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315401.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2178"/>
    <dbReference type="ESTHER" id="nitwn-q3sqk9">
        <property type="family name" value="6_AlphaBeta_hydrolase"/>
    </dbReference>
    <dbReference type="MEROPS" id="S33.010"/>
    <dbReference type="EnsemblBacteria" id="ABA05432">
        <property type="protein sequence ID" value="ABA05432"/>
        <property type="gene ID" value="Nwi_2178"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2178"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_50_5_5"/>
    <dbReference type="OMA" id="DIPNRDH"/>
    <dbReference type="OrthoDB" id="1196738at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="21"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="250" mass="26840" checksum="2F9BE9A5CCC8F80C" modified="2005-10-11" version="1">MPSFHHGEVEVSYLDEGEGDPVVLVHGFASSKNVNWVYPTWVSELRKNGFRVIALDNRGHGDSTKLYDPEDYHIGTMASDVTALMDHLGVARADVMGYSLGARIMGILAHTRPERVRSGIFGGLGVGLIEGGGPGESVARALEAASLDDVADPFGRTFRAFADQTRSDRRALAACLRGSRRLMTEAEAASISVPTLIAVGSKDEIAGSAQALAKIIPGAEVLDIPNRDHMRAVGDKVYKDGVLKFLSRRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQQ6</accession>
    <name>Q3SQQ6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2130</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315356.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2130"/>
    <dbReference type="EnsemblBacteria" id="ABA05385">
        <property type="protein sequence ID" value="ABA05385"/>
        <property type="gene ID" value="Nwi_2130"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2130"/>
    <dbReference type="eggNOG" id="ENOG502Z7S6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054190_0_0_5"/>
    <dbReference type="OMA" id="ELLWGTV"/>
    <dbReference type="OrthoDB" id="549473at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019627">
        <property type="entry name" value="YAcAr"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10686">
        <property type="entry name" value="YAcAr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="307" mass="34308" checksum="1B001164B4972535" modified="2005-10-11" version="1">MTSDDDFEPHHSSSPTDHVLTELQLYGFRPFQGEADPRPLPEGNAVAGAIADIFDALVATLGDTRLEPDLEDLLWSTVNVFHRAADRIARELDDNEQAQRRSQQEQDGTEIKSVELERLIAEGQTLIERRDAFELMRDQACEHFERHTGSAWRPRSGSLVNHRAMTAAMIDSRDFLAAKKRAETEVMLPPGPKVALSGGLDFNDHRLIWAKLDQVHAKHPDMVLMHGGSPKGAERIAARWADHRNVPQIAFKPDWTKHAKAAPFKRNDAMLEVLPIGVMIFPGTGIQENIADKARKLGIPVWRFGGA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-04-08" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR25</accession>
    <name>PYRB_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartate carbamoyltransferase</fullName>
            <ecNumber evidence="1">2.1.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Aspartate transcarbamylase</fullName>
            <shortName evidence="1">ATCase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrB</name>
        <name type="ordered locus">Nwi_2007</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>carbamoyl phosphate + L-aspartate = H(+) + N-carbamoyl-L-aspartate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20013"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. ATCase family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="ABA05266" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.1.3.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05266.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_041344999.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SR25"/>
    <dbReference type="SMR" id="Q3SR25"/>
    <dbReference type="STRING" id="323098.Nwi_2007"/>
    <dbReference type="EnsemblBacteria" id="ABA05266">
        <property type="protein sequence ID" value="ABA05266"/>
        <property type="gene ID" value="Nwi_2007"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2007"/>
    <dbReference type="eggNOG" id="COG0540">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043846_2_0_5"/>
    <dbReference type="OrthoDB" id="1844275at2"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00116"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004070">
        <property type="term" value="F:aspartate carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:cellular amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00001">
        <property type="entry name" value="Asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002082">
        <property type="entry name" value="Asp_carbamoyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF16">
        <property type="entry name" value="PTHR11405:SF16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="SSF53671"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00670">
        <property type="entry name" value="asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000329109" description="Aspartate carbamoyltransferase">
        <location>
            <begin position="1"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="317" mass="34367" checksum="33D1F7F48AC6FB7F" modified="2008-04-08" version="2">MTSSAKSSFVLGHRHLLGIEGLSVADITGLLDLSEEYVELNRQVDKKRTSLRGLTQVNLFFEASTRTQSSFEIAGKRLGADVMNMSVSSLSTRKGETLVDTAMTLNAMHPDILVMRHSASGAVELLARKVDGSVVNAGDGAHEHPTQALLDALTIRRNKGRLDGLLVAICGDVLHSRVARSNIILLNAMGARVRVVAPSTLLPPGIERMGVEVARDMREGLDGADIVMMLRLQRERMSGSFVPSSSEYFHYFGLDQKKLAYAKPNALVMHPGPMNRGVEIDSIVADGTQSLIREQVEMGVAVRMAVLEALARNLPNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR51</accession>
    <name>Q3SR51_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Carboxysome structural polypeptide</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1981</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315227.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1981"/>
    <dbReference type="EnsemblBacteria" id="ABA05240">
        <property type="protein sequence ID" value="ABA05240"/>
        <property type="gene ID" value="Nwi_1981"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1981"/>
    <dbReference type="eggNOG" id="COG4577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064903_5_3_5"/>
    <dbReference type="OMA" id="VIARPHA"/>
    <dbReference type="OrthoDB" id="1802372at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031469">
        <property type="term" value="C:bacterial microcompartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020808">
        <property type="entry name" value="Bact_microcomp_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000249">
        <property type="entry name" value="BMC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037233">
        <property type="entry name" value="CcmK-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044872">
        <property type="entry name" value="CcmK/CsoS1_BMC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00936">
        <property type="entry name" value="BMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00877">
        <property type="entry name" value="BMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143414">
        <property type="entry name" value="SSF143414"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01139">
        <property type="entry name" value="BMC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51930">
        <property type="entry name" value="BMC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1283">Bacterial microcompartment</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BMC" evidence="2">
        <location>
            <begin position="8"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="98" mass="9997" checksum="2CA19F9E4087C624" modified="2005-10-11" version="1">MAEIMGIALGMIETRGLVPAIEAADAMTKAAEVRLISRQFVGGGYVTVLVRGETGAVNAAVRAGADACERVGDGLVAAHIIARVHSEVEGILPKTPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR61</accession>
    <name>Q3SR61_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1971</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05230">
        <property type="protein sequence ID" value="ABA05230"/>
        <property type="gene ID" value="Nwi_1971"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1971"/>
    <dbReference type="HOGENOM" id="CLU_1766092_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="147" mass="15490" checksum="BEAB0F00604C6F15" modified="2005-10-11" version="1">MRRGRMERAGGTGAIGQALLVGASWKRGPARGGVRNTKWRSGLDAAVLFPVRDRHPDGPKPQLAGSVEAATDAGIEPGALRCGQPHIDDVKIAAGSAPHLARTPQLVTATDRHDVVGNRREIATSVQFAARPAGYLPPAIEPVSRPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR95</accession>
    <name>Q3SR95_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CRISPR-associated endonuclease Cas1</fullName>
            <ecNumber evidence="8">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cas1</name>
        <name evidence="10" type="ordered locus">Nwi_1936</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as a dsDNA endonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer, forms a heterotetramer with a Cas2 homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the CRISPR-associated endonuclease Cas1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="8"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315189.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1936"/>
    <dbReference type="EnsemblBacteria" id="ABA05196">
        <property type="protein sequence ID" value="ABA05196"/>
        <property type="gene ID" value="Nwi_1936"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1936"/>
    <dbReference type="eggNOG" id="COG1518">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052779_1_0_5"/>
    <dbReference type="OMA" id="AYKLIKH"/>
    <dbReference type="OrthoDB" id="1581397at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004520">
        <property type="term" value="F:endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09721">
        <property type="entry name" value="Cas1_I-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01470">
        <property type="entry name" value="Cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002729">
        <property type="entry name" value="CRISPR-assoc_Cas1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042206">
        <property type="entry name" value="CRISPR-assoc_Cas1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019856">
        <property type="entry name" value="CRISPR-assoc_Cas1_DVULG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042211">
        <property type="entry name" value="CRISPR-assoc_Cas1_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01867">
        <property type="entry name" value="Cas_Cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00287">
        <property type="entry name" value="cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03640">
        <property type="entry name" value="cas1_DVULG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA05196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="344" mass="38577" checksum="CC8874A7D8166954" modified="2005-10-11" version="1">MKKLLNTVYVTTEGVALRKDGENLVAEVEGEERARVPLHMLASLVVFGPIHISPALMAACAGAGIAIALLDRNGRFRARVEGPVTGNVLLRRAQYRVSEAPEPVVRSILVGKVANQRSVLMRALRDHREELEPGDRDAITAVTERLAFILRRVERSDGGLDVLRGAEGEAANLYFSVFDNLLRVPDPDLRWKGRSRRPPLDPINALLSFLYTLLTHDCRSACESVGLDPAVGFLHRDRPGRPSLALDLMEELRPVLTDRLALSLINRRQLRAGDFEKRDGGAVVMSDDARRTVLTAWQERKKEERLHPFLQEKAPLGLVPYLQAQMLARHLRGDLDAYPPWFWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="132" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRB4</accession>
    <name>Q3SRB4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">GTPase Era</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">era</name>
        <name evidence="12" type="ordered locus">Nwi_1917</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">An essential GTPase that binds both GDP and GTP, with rapid nucleotide exchange. Plays a role in 16S rRNA processing and 30S ribosomal subunit biogenesis and possibly also in cell cycle regulation and energy metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cell inner membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 8 9">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Era GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1917"/>
    <dbReference type="EnsemblBacteria" id="ABA05177">
        <property type="protein sequence ID" value="ABA05177"/>
        <property type="gene ID" value="Nwi_1917"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1917"/>
    <dbReference type="eggNOG" id="COG1159">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038009_1_1_5"/>
    <dbReference type="OMA" id="WAEVDVI"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04163">
        <property type="entry name" value="Era"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00367">
        <property type="entry name" value="GTPase_Era"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030388">
        <property type="entry name" value="G_ERA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005662">
        <property type="entry name" value="GTP-bd_Era"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42698">
        <property type="entry name" value="PTHR42698"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00436">
        <property type="entry name" value="era"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51713">
        <property type="entry name" value="G_ERA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Era-type G" evidence="11">
        <location>
            <begin position="9"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="KH type-2" evidence="10">
        <location>
            <begin position="207"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="G1" evidence="8">
        <location>
            <begin position="17"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="G2" evidence="8">
        <location>
            <begin position="43"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="G3" evidence="8">
        <location>
            <begin position="64"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="G4" evidence="8">
        <location>
            <begin position="126"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="G5" evidence="8">
        <location>
            <begin position="155"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="17"/>
            <end position="24"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="64"/>
            <end position="68"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="126"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA05177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="303" mass="33480" checksum="039E1798182DC215" modified="2005-10-11" version="1">MSETGPTSRCGFVALIGAPNVGKSTLVNALVGSKVTIVSRKVQTTRALIRGIVIENNAQIILVDTPGIFVPKRRLDRAMVSTAWSGAHDADLVCVLLDARAGIDEEADAILTKLAGVAHPKILVLNKIDLVRREKLLALAGSANERLGFEETFMVSALSGDGVDDLRLALAGRVPEGPFHYPEDQMSDAPLRHLAAEITREKIYRHLHQELPYQSTVETDTWTERNDKSVRIEQTIFVERDSQRKIVLGKNGATIKSIGASSRKEIGEIIGQPVHLFLFVKVREKWGDDPNRYREMGLEFPKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRE3</accession>
    <name>Q3SRE3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NADH-quinone oxidoreductase subunit A</fullName>
            <ecNumber evidence="6">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">NADH dehydrogenase I subunit A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">NDH-1 subunit A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">NUO1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">nuoA</name>
        <name evidence="8" type="ordered locus">Nwi_1888</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell inner membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the complex I subunit 3 family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315144.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1888"/>
    <dbReference type="EnsemblBacteria" id="ABA05148">
        <property type="protein sequence ID" value="ABA05148"/>
        <property type="gene ID" value="Nwi_1888"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1888"/>
    <dbReference type="eggNOG" id="COG0838">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119549_3_1_5"/>
    <dbReference type="OMA" id="RFPVKYY"/>
    <dbReference type="OrthoDB" id="1748431at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01394">
        <property type="entry name" value="NDH1_NuoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023043">
        <property type="entry name" value="NAD(P)H_OxRDtase_bac/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000440">
        <property type="entry name" value="NADH_UbQ/plastoQ_OxRdtase_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038430">
        <property type="entry name" value="NDAH_ubi_oxred_su3_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11058">
        <property type="entry name" value="PTHR11058"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00507">
        <property type="entry name" value="Oxidored_q4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 7" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 7" id="KW-0874">Quinone</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0830">Ubiquinone</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA05148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="121" mass="13533" checksum="4D508FD7C8FB6983" modified="2005-10-11" version="1">MSGILQNYLPLVVFIGIAAAIGVALLVAPFIVAYKQPDPEKLSAYECGFNAFDDARMKFDVRFYLVAILFIIFDLEVAFLFPWAVTFGKLGVAGFWSMMVFLGVLTVGFAYEWKKGALEWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRG0</accession>
    <name>Q3SRG0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Methylmalonyl-CoA epimerase</fullName>
            <ecNumber evidence="3">5.1.99.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1871</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methylmalonyl-CoA epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.99.1" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315127.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1871"/>
    <dbReference type="EnsemblBacteria" id="ABA05131">
        <property type="protein sequence ID" value="ABA05131"/>
        <property type="gene ID" value="Nwi_1871"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1871"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046006_5_0_5"/>
    <dbReference type="OMA" id="IHHICYE"/>
    <dbReference type="OrthoDB" id="1335734at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004493">
        <property type="term" value="F:methylmalonyl-CoA epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07249">
        <property type="entry name" value="MMCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017515">
        <property type="entry name" value="MeMalonyl-CoA_epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03081">
        <property type="entry name" value="metmalonyl_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="2">
        <location>
            <begin position="4"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="134" mass="14300" checksum="2A800A10857F5CFA" modified="2005-10-11" version="1">MLGRLNHVAIAVRDAEKAAKIYGAAFGAEISAPLPLPEHGVITVFAILPNTKIEFIQPLGETSPIAKFVERNADGGIHHVCYEVPDIVATRDILIAEGARVLGDGEPKIGAHGKPVLFFHPKDFSGALVEIEQS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-01-23" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRG8</accession>
    <name>LOLD_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lipoprotein-releasing system ATP-binding protein LolD</fullName>
            <ecNumber evidence="1">7.6.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lolD</name>
        <name type="ordered locus">Nwi_1863</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the ABC transporter complex LolCDE involved in the translocation of mature outer membrane-directed lipoproteins, from the inner membrane to the periplasmic chaperone, LolA. Responsible for the formation of the LolA-lipoprotein complex in an ATP-dependent manner.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of two ATP-binding proteins (LolD) and two transmembrane proteins (LolC and LolE).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. Lipoprotein translocase (TC 3.A.1.125) family.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315119.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRG8"/>
    <dbReference type="SMR" id="Q3SRG8"/>
    <dbReference type="STRING" id="323098.Nwi_1863"/>
    <dbReference type="EnsemblBacteria" id="ABA05123">
        <property type="protein sequence ID" value="ABA05123"/>
        <property type="gene ID" value="Nwi_1863"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1863"/>
    <dbReference type="eggNOG" id="COG1136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_5"/>
    <dbReference type="OMA" id="FVYQSHR"/>
    <dbReference type="OrthoDB" id="1181903at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042953">
        <property type="term" value="P:lipoprotein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015854">
        <property type="entry name" value="ABC_transpr_LolD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24220:SF654">
        <property type="entry name" value="PTHR24220:SF654"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51244">
        <property type="entry name" value="LOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000272112" description="Lipoprotein-releasing system ATP-binding protein LolD">
        <location>
            <begin position="1"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="11"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="47"/>
            <end position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01708"/>
        </source>
    </evidence>
    <sequence length="232" mass="25529" checksum="C4C0F12581824BF2" modified="2005-10-11" version="1">MEQGKKETPTVYLHDIKREYTQGETRLTILDSTKLALWAGQSVALVAPSGSGKSTLLHIAGLLESPDAGEVYIGGIATSGLSDVERTQIRRTDIGFVYQSHRLLPEFTALENVMLPQMIRGLKRAETVKRAQEILAYLGLEDRVTHRPAELSGGEQQRVAIARAVANAPRVLLADEPTGNLDPQTADHVFNALMQLVKATRVSMLIATHNMELADRMDRRVSLENGHVVELD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRH3</accession>
    <name>EFTS_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Elongation factor Ts</fullName>
            <shortName evidence="1">EF-Ts</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tsf</name>
        <name type="ordered locus">Nwi_1858</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the EF-Ts family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315114.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRH3"/>
    <dbReference type="SMR" id="Q3SRH3"/>
    <dbReference type="STRING" id="323098.Nwi_1858"/>
    <dbReference type="EnsemblBacteria" id="ABA05118">
        <property type="protein sequence ID" value="ABA05118"/>
        <property type="gene ID" value="Nwi_1858"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1858"/>
    <dbReference type="eggNOG" id="COG0264">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047155_2_0_5"/>
    <dbReference type="OMA" id="DAGMMDC"/>
    <dbReference type="OrthoDB" id="1405357at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00050">
        <property type="entry name" value="EF_Ts"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036402">
        <property type="entry name" value="EF-Ts_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001816">
        <property type="entry name" value="Transl_elong_EFTs/EF1B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014039">
        <property type="entry name" value="Transl_elong_EFTs/EF1B_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018101">
        <property type="entry name" value="Transl_elong_Ts_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11741">
        <property type="entry name" value="PTHR11741"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00889">
        <property type="entry name" value="EF_TS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="SSF46934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54713">
        <property type="entry name" value="SSF54713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00116">
        <property type="entry name" value="tsf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01126">
        <property type="entry name" value="EF_TS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01127">
        <property type="entry name" value="EF_TS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0251">Elongation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000241499" description="Elongation factor Ts">
        <location>
            <begin position="1"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Involved in Mg(2+) ion dislocation from EF-Tu" evidence="1">
        <location>
            <begin position="80"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00050"/>
        </source>
    </evidence>
    <sequence length="307" mass="32103" checksum="C70E680C94E1DAEE" modified="2005-10-11" version="1">MATITAAMVKELREITGVGMMDCKQALTENNGDMQAAIDWLRKKGLSKAAKKAGRVAAEGLIGVVTSGNKGVVVEVNSETDFVARNEQFQGLVKMVAQVALSVGADVEVIKAAEVGNATVATTISDAIATIGENMTLRRANSLEVPKGIVAGYVHNAVIDGAGKLGVIVALESAGNADELAALGRQVAMHIASANPVAVDPSGVDPEVVKREKDVLADKFRQQGKPEAMIEKITESGLKTFFKEQTLLEQPFIFDDKRSVGQALKEAEGRVGAPVRIAGFVRYALGEGIEKAESDFAAEVAAAAGQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRM5</accession>
    <name>Q3SRM5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glycoside hydrolase, family 3</fullName>
            <ecNumber evidence="4">3.2.1.52</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1805</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.2.1.52" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315062.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1805"/>
    <dbReference type="CAZy" id="GH3">
        <property type="family name" value="Glycoside Hydrolase Family 3"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05066">
        <property type="protein sequence ID" value="ABA05066"/>
        <property type="gene ID" value="Nwi_1805"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1805"/>
    <dbReference type="eggNOG" id="COG1472">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008392_0_0_5"/>
    <dbReference type="OMA" id="WQMAAEM"/>
    <dbReference type="OrthoDB" id="949956at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004563">
        <property type="term" value="F:beta-N-acetylhexosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102148">
        <property type="term" value="F:N-acetyl-beta-D-galactosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_hydro_3" evidence="3">
        <location>
            <begin position="22"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="338" mass="35888" checksum="904E0D85A334AB9F" modified="2005-10-11" version="1">MSTRAFITGASGLGLTMQEREFLRAGRPWGFILFKRNIDNPLQVARLVQEFREAIEDGDAPVLIDQEGGRVQRLGPPHWPVYPAGATFGALYDRDQAAGLEAARLGAQLIAADLADLGITVDCLPLADVPVPGADAVIGDRAYGTDPLKVATIARAVTEGLEQGGVLPVLKHIPGHGRATADTHHRLPIVDTLKDELEAVDFAAFRPLSDLPMAMTAHVVFSAIDATQPATTSATIIEQVIRGSIGFQGLLMSDDVSMNALTGSIAQRTRAIVAAGCDMVLHCNGQLDEMRQVASEAPELSGVALDRARRALAARRPPQPFDREAARAKLDALTAASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRM7</accession>
    <name>Q3SRM7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Condensin subunit ScpB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_1803</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315060.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1803"/>
    <dbReference type="EnsemblBacteria" id="ABA05064">
        <property type="protein sequence ID" value="ABA05064"/>
        <property type="gene ID" value="Nwi_1803"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1803"/>
    <dbReference type="eggNOG" id="COG1386">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045647_3_0_5"/>
    <dbReference type="OMA" id="WIRVVGY"/>
    <dbReference type="OrthoDB" id="1771906at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051304">
        <property type="term" value="P:chromosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005234">
        <property type="entry name" value="ScpB_csome_segregation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34298">
        <property type="entry name" value="PTHR34298"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04079">
        <property type="entry name" value="SMC_ScpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00281">
        <property type="entry name" value="TIGR00281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="194"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="248" mass="26831" checksum="D1195A2294080D58" modified="2005-10-11" version="1">MASLAEIRVERLEEPETELPASRPEELRLLEALLFASSEPLDQSALAKRMPDGVDIKSALAQLQADYASRGVNLVRIANKWTFRTAGDLAWLMTRESTETRRLSRAAIEVLAIIAYHQPVTRAEIEEIRGVVTSKGTLDVLLETGWIKPRGRRKTPGRPLTFGTTEAFLSQFSLENLGDLPGLEELKGTGLLDSRLPGGFSVPTPSDDPALRDDEDPLDAGDLELALVPALEAESDNSSSGGTAPTGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRP9</accession>
    <name>Q3SRP9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1781</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05042">
        <property type="protein sequence ID" value="ABA05042"/>
        <property type="gene ID" value="Nwi_1781"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1781"/>
    <dbReference type="HOGENOM" id="CLU_2586130_0_0_5"/>
    <dbReference type="OMA" id="NDGFEME"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="81" mass="8814" checksum="868D95CB1E8E8E7F" modified="2005-10-11" version="1">MMAEDNFEVETEGSAIAAFTLAQFAFWGLIESGVISTEKAADMLEQGVTALSKGDLANRKASQMLQTILDMVQRNQRSSVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRS2</accession>
    <name>Q3SRS2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1758</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1758"/>
    <dbReference type="EnsemblBacteria" id="ABA05019">
        <property type="protein sequence ID" value="ABA05019"/>
        <property type="gene ID" value="Nwi_1758"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1758"/>
    <dbReference type="eggNOG" id="COG4765">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_791475_0_0_5"/>
    <dbReference type="OMA" id="PAQKIVN"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019225">
        <property type="entry name" value="DUF2155"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09923">
        <property type="entry name" value="DUF2155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="339"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="397" mass="42725" checksum="18EE6D6CB6F67B19" modified="2005-10-11" version="1">MRPSLLPDGNQQRGSGCLDAFHVNRPRFRSITLLPTESSELSRCRQWTRSRSPFPRRNRRVNRIAASGLEPLIPVQWSGPDSSLTKPRMIFRTIVIAGCAAFAGVALVAAPPSARAQIGNIFSDPPPRPPGAIPQGQAAPEDDEEEVPELPPEGRVLPAPMRPPPGQGVPPPGPVQSQPLPPPSGTTVTPQNAPGGAAVAPPSPRQAPEQNAARPAPPAPATLQPGDEVVTEAPAERIVNKKAVFSGLDKITGRIIHFDEDIGETVQFGALRVKTSACYTRPATEAANTDAFVEVDEITLQGEVKRIFSGWMFASSPGLHGVEHPIYDVWLTDCKDPETTVIAEQPDQPKPTPPPPARRSRQSTRQSRQPAPPPQPAPVPPAQRQPQSNPFFPPFQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRW5</accession>
    <name>Q3SRW5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptide chain release factor 2</fullName>
            <shortName evidence="4">RF-2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">prfB</name>
        <name evidence="7" type="ordered locus">Nwi_1715</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="4">Methylated by PrmC. Methylation increases the termination efficiency of RF2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1715"/>
    <dbReference type="EnsemblBacteria" id="ABA04976">
        <property type="protein sequence ID" value="ABA04976"/>
        <property type="gene ID" value="Nwi_1715"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1715"/>
    <dbReference type="eggNOG" id="COG1186">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036856_6_0_5"/>
    <dbReference type="OMA" id="YVFHPYQ"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00094">
        <property type="entry name" value="Rel_fac_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005139">
        <property type="entry name" value="PCRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004374">
        <property type="entry name" value="PrfB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03462">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00937">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="SSF75620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00020">
        <property type="entry name" value="prfB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00745">
        <property type="entry name" value="RF_PROK_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0488">Methylation</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RF_PROK_I" evidence="6">
        <location>
            <begin position="189"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="33"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="modified residue" description="N5-methylglutamine" evidence="4">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="322" mass="35582" checksum="1D03E6E5EFE34069" modified="2005-10-11" version="1">MQERTSLEDSLAGIGKVERELEDQIGMIELGEAENDEAVIDESENALKALKKEVDRRELEALLSGEADRFDTYLEVHAGAGGTESQDWAAMLLRMYTRWAEKRGFKVEYLEETPGEEAGIKSATVQVSGHNAYGWLKTEAGVHRLVRISPFDSNARRHTSFSSVAIFPVVDDSIKIDIKESDVRTDTMRSGGAGGQHVNKTESAVRLTHVPTGVAVVCQAGRSQHKNRAQAWDMLRARLYEIELKRREEQAAADQAAKTDIGWGHQIRSYVLQPYQMVKDLRTGVQTSDTSGVLDGNLDDFMAATLAQRAFGTTPGAVEDVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS39</accession>
    <name>Q3SS39_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">L-lysine 2,3-aminomutase</fullName>
            <ecNumber evidence="10">5.4.3.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_1641</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="5.4.3.2" evidence="10"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314901.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1641"/>
    <dbReference type="EnsemblBacteria" id="ABA04902">
        <property type="protein sequence ID" value="ABA04902"/>
        <property type="gene ID" value="Nwi_1641"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1641"/>
    <dbReference type="eggNOG" id="COG1509">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032161_2_0_5"/>
    <dbReference type="OMA" id="PIWLNTH"/>
    <dbReference type="OrthoDB" id="557227at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050066">
        <property type="term" value="F:lysine 2,3-aminomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022447">
        <property type="entry name" value="Lys_aminomutase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003739">
        <property type="entry name" value="Lys_aminomutase/Glu_NH3_mut"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30538">
        <property type="entry name" value="PTHR30538"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004911">
        <property type="entry name" value="DUF160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01070">
        <property type="entry name" value="PLP-dependent"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03822">
        <property type="entry name" value="AblA_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00238">
        <property type="entry name" value="TIGR00238"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6 7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="9">
        <location>
            <begin position="96"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="8">
        <location>
            <position position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR004911-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR603739-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA04902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="366" mass="39673" checksum="3AFE0D8F94D2F6F5" modified="2005-10-11" version="1">MSARITKGIASTLRRPEDLIAHGLAPAAALADLEKVAARYAIAITPEVANLIDPADPDDPIARQYLPSADELAAQAYERADPIGDHARSPVDGIVHRYPDRVLLKLVHVCAVYCRFCFRREMVGPAKETALSKSATAAALDYIRSHPEVWEVILTGGDPLMLSPRRLAEIMAELAAIDHVRIVRIHTRVPVADPARVTDEMAAALRTDGATTWLALHANHPRELTAAARAACARIIDAGIPMVSQSVLLRGVNDDAATLEALMRAFVQCRIKPYYLHHGDLAPGTAHLRTTLEQGQALMRELRGRVSGLCQPDYVLDIPGGYGKSPVGPGYMSPSDLISGAGEHRPELHYLITDYCGGVHLYPPKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS47</accession>
    <name>Q3SS47_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Phage integrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_1633</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1633"/>
    <dbReference type="EnsemblBacteria" id="ABA04894">
        <property type="protein sequence ID" value="ABA04894"/>
        <property type="gene ID" value="Nwi_1633"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1633"/>
    <dbReference type="eggNOG" id="COG0582">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_640806_0_0_5"/>
    <dbReference type="OMA" id="KEDRTHA"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="7">
        <location>
            <begin position="57"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="6">
        <location>
            <begin position="183"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="119"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="129"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA04894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="362" mass="40496" checksum="D7B5386694383976" modified="2005-10-11" version="1">MRLYAGEQSYRTETLATADDFSDADGVAILDWRQAQTLARKRMADYARTEAGIAKPLTVADAMATYLEYLETKTKSGREGRYAAEAFILPVFGSVEVAKLTKEQIERWHAALAKNPARIRTKNGRRQRFKSTTHPENDRRRKSTANRILTTLKAALNLAWRDGKVPTDAAWRRVTPFESVDSARVRYLSMTEIRRLLEACSPAFKHLVRVALETGCRYGELIALRVEDFDADAGTLHVRDSKSGKPRHVVLTQEGAAYVAEIAKGRSPAAMLLVKENGEPWQRDHQADPLRNACKNAKIDPAINFHALRHSWASHAVMRGVSLQIVARQLGHTTTRVTEAHYAHLGPSFVADAIRANAPRFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSF7</accession>
    <name>Q3SSF7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Secretion protein HlyD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_1523</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314790.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1523"/>
    <dbReference type="EnsemblBacteria" id="ABA04784">
        <property type="protein sequence ID" value="ABA04784"/>
        <property type="gene ID" value="Nwi_1523"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1523"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018816_2_0_5"/>
    <dbReference type="OMA" id="NTAQMRT"/>
    <dbReference type="OrthoDB" id="1432684at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032317">
        <property type="entry name" value="HlyD_D23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006143">
        <property type="entry name" value="RND_pump_MFP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16576">
        <property type="entry name" value="HlyD_D23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01730">
        <property type="entry name" value="RND_mfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="HlyD_D23" evidence="4">
        <location>
            <begin position="94"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="396"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="412"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="457" mass="48792" checksum="1BB1353972C52F56" modified="2005-10-11" version="1">MLFRPGLDDTVKAAGDGLSGARAASDRRRPLRIAITLLIVGGLAYLGWTSFAQKKRDGSWERPDRAVPVLAATPRIQDVPVYLDGVGAVRALSTVTVRSQVNGKLIAVNFTEGQDVKKGDVLGEIEPVIYQAQYDQAVAKKAQDEAQLANMRLDLKRYEQLAASNAGSKQQADTQRAVVAQQEALVKADQAAIDNAQAMLGFTRIVAPISGRVGLRQVDRGNIINAADATGLVIITQLQPISVQFSLPQQQITRVNAAAAKGEIEVDVFGNDGTTVVDTGKLIGIDNQVDPTTGTLRLRAAFSNEHFQLWPGQFVNVRIKVETLSQATVIPTSAVQRGPLGTFSYVIGDDDIVKARPVTVRQQDEKDAVIAKGIAPTDRVVTTGFANLSDGAKVTVGQSDQVPTADLAPPARQDRRGARRGGDGSDRRGRRSERGQNQSGSEPQESGQSGAGPKPRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSJ5</accession>
    <name>Q3SSJ5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phage portal protein, HK97</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1485</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1485"/>
    <dbReference type="EnsemblBacteria" id="ABA04746">
        <property type="protein sequence ID" value="ABA04746"/>
        <property type="gene ID" value="Nwi_1485"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1485"/>
    <dbReference type="eggNOG" id="COG4695">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033789_5_0_5"/>
    <dbReference type="OMA" id="NVPAHMI"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006944">
        <property type="entry name" value="Phage/GTA_portal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006427">
        <property type="entry name" value="Portal_HK97"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04860">
        <property type="entry name" value="Phage_portal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01537">
        <property type="entry name" value="portal_HK97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="378"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="381"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="399" mass="42934" checksum="A7B2D2A5ABB6C51D" modified="2005-10-11" version="1">MYLEQATLKLWPFSRTTVETKSLSDPDEAIVAILSGGSTYGPVHPLSEPAVSAAVTTISNAAATLDLHLVKRDDTSADVKHPALDLLRGHVNEWTSGSVLIRDLIAQALTADAGGIAWVNKSSDGRPLEIIRYSAGRITVEYAGDGSGRPSYTVNGRSVPASDIIHIRGPFSKCPVSLAYPTISAAVAMSRYVEFLFKRMARPGGVVEVPSGAGEKAVQNMIAGWNAAYGGPDNAGGTAFLFDGATFKQIALASTDAQFVENRRWQLEDIARAFNISSVMLGDLAKSSYANAWQKYREFLSVTLMPWLKALESAFDRALLTDDERALYAFKFDIDDLTRVDLEKRATAISSLVASRVLNPNEARTWLDTGLAPYAGGNEFANPNTGASQPGSQEQPVND</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSN0</accession>
    <name>Q3SSN0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GTPase HflX</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">GTP-binding protein HflX</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">hflX</name>
        <name evidence="9" type="ordered locus">Nwi_1450</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">GTPase that associates with the 50S ribosomal subunit and may have a role during protein synthesis or ribosome biogenesis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer. Associates with the 50S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="5">May associate with membranes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. HflX GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1450"/>
    <dbReference type="EnsemblBacteria" id="ABA04711">
        <property type="protein sequence ID" value="ABA04711"/>
        <property type="gene ID" value="Nwi_1450"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1450"/>
    <dbReference type="eggNOG" id="COG2262">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019597_1_0_5"/>
    <dbReference type="OMA" id="AEMKTGR"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01878">
        <property type="entry name" value="HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00900">
        <property type="entry name" value="GTPase_HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030394">
        <property type="entry name" value="G_HFLX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032305">
        <property type="entry name" value="GTP-bd_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016496">
        <property type="entry name" value="GTPase_HflX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025121">
        <property type="entry name" value="GTPase_HflX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042108">
        <property type="entry name" value="GTPase_HflX_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045498">
        <property type="entry name" value="HflX_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10229">
        <property type="entry name" value="PTHR10229"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16360">
        <property type="entry name" value="GTP-bdg_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13167">
        <property type="entry name" value="GTP-bdg_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19275">
        <property type="entry name" value="HflX_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006809">
        <property type="entry name" value="GTP-binding_hflX_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03156">
        <property type="entry name" value="GTP_HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51705">
        <property type="entry name" value="G_HFLX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hflx-type G" evidence="8">
        <location>
            <begin position="209"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="215"/>
            <end position="222"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="240"/>
            <end position="244"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="262"/>
            <end position="265"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="331"/>
            <end position="334"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR006809-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR006809-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA04711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="442" mass="48925" checksum="815706C76C36D188" modified="2005-10-11" version="1">MRGEPTGRAIVIGPYVRTRGGDAETPRRSLRNTDARLDEAAGLAKAIDLHVIQAIVAPVSQIRPATYLGKGKVEELQGLIAADGAGLVVMDCALSPIQQRNLEKAWSTKVLDRTGLILEIFGRRAKTKEGALQVELAHLNYQRSRLVRSWTHLERQRGGFGFMGGPGETQIEADRRLIGDRITRLENELKKVQATRRLHRAGRQRVPYRVVALVGYTNAGKSTLFNRLTRADVQAADMLFATLDPTLRSLALPHGGKAMLSDTVGFISNLPTQLVAAFRATLEEVMEADVILHVRDISHEDTEPQQHDVEAVLHQLGIDPDGPARMIEVWNKIDRFDADQRKDLENVAARRSTDVPRFLVSAETGEGVETLLAAIEDRLAATRTTLDLSVDASDGAAISWLHRNSEVLAKELRDGRYDMTVRVDETKRDILVNRYGAAPRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSP5</accession>
    <name>Q3SSP5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1435</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1435"/>
    <dbReference type="EnsemblBacteria" id="ABA04696">
        <property type="protein sequence ID" value="ABA04696"/>
        <property type="gene ID" value="Nwi_1435"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1435"/>
    <dbReference type="eggNOG" id="COG3827">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078707_0_0_5"/>
    <dbReference type="OMA" id="YAGVEFP"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019632">
        <property type="entry name" value="DUF2497"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10691">
        <property type="entry name" value="DUF2497"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="239" mass="25292" checksum="5D0D18755CCD3B64" modified="2005-10-11" version="1">MKILISLEAGALARAVTDVELDMTQPAKVQEPSMEEILASIRRIIADDEKPAAAGKAAVADVVEARPRTAPAPKPAAPANADAANSQDDIDAMLANLDAAATGKEGRPAEAEEVFELTDEMAVPDARQDAFQKIEPQDDLEFAEATQVAAASPDSAAIPPSNPNAAPAARILSHSTVSAVETAFNSLASTVLTNNARTLEDLVKEMLRPMLKSWLDDNLPGLVERIVKAEIERVSRGRP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSW6</accession>
    <name>RL3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplC</name>
        <name type="ordered locus">Nwi_1364</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Forms a cluster with proteins L14 and L19.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Methylated by PrmB.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314641.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSW6"/>
    <dbReference type="SMR" id="Q3SSW6"/>
    <dbReference type="STRING" id="323098.Nwi_1364"/>
    <dbReference type="EnsemblBacteria" id="ABA04625">
        <property type="protein sequence ID" value="ABA04625"/>
        <property type="gene ID" value="Nwi_1364"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1364"/>
    <dbReference type="eggNOG" id="COG0087">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044142_2_0_5"/>
    <dbReference type="OMA" id="KRMAGRY"/>
    <dbReference type="OrthoDB" id="1270636at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01325_B">
        <property type="entry name" value="Ribosomal_L3_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_L3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019927">
        <property type="entry name" value="Ribosomal_L3_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019926">
        <property type="entry name" value="Ribosomal_L3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11229">
        <property type="entry name" value="PTHR11229"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03625">
        <property type="entry name" value="L3_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00474">
        <property type="entry name" value="RIBOSOMAL_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0488">Methylation</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000241375" description="50S ribosomal protein L3">
        <location>
            <begin position="1"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="140"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="212"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="modified residue" description="N5-methylglutamine" evidence="1">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="247" mass="26263" checksum="63BBE825EA96C0B1" modified="2005-10-11" version="1">MRSGVIAQKVGMTRVFTEAGEHIPVTVLKLSNCQVLGHRTSEKNGYVALQLGSGARKTVYMPKAERGQFAVAKVAPKRKVAEFRVSEDSLIPVGAEIQADHFVVGQFVDVTGTSVGKGYAGGMKRWNFGGLRATHGVSISHRSIGSTGGRQDPGKTFKNKKMPGHMGVDRITTLNLRVVQTDVERGLILVEGAVPGSKGGWIAVRDAVKKPLPKEAPKPGKFKVVGDAQAVDEDKAPADTPAEKEGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSX4</accession>
    <name>Q3SSX4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH crp-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1356</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314635.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1356"/>
    <dbReference type="EnsemblBacteria" id="ABA04617">
        <property type="protein sequence ID" value="ABA04617"/>
        <property type="gene ID" value="Nwi_1356"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1356"/>
    <dbReference type="eggNOG" id="COG0664">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077340_0_0_5"/>
    <dbReference type="OMA" id="ACNRHHT"/>
    <dbReference type="OrthoDB" id="1596937at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012318">
        <property type="entry name" value="HTH_CRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13545">
        <property type="entry name" value="HTH_Crp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="SSF51206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51063">
        <property type="entry name" value="HTH_CRP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH crp-type" evidence="2">
        <location>
            <begin position="143"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="236" mass="26954" checksum="F21CD110F3511CE4" modified="2005-10-11" version="1">MVHGPKNSLLASIPDTEWIEVQKLCEFVKLPLDRVLYEPRGPFDYVYFPETAIISNVAPLKVDKTAETSTTGREGMINVGAIMGDDQSMHRAVVQVSGDSIRIAFQDFQELQARLPVFRRKLNAYSRAFLAQVMQSVACNASHTLLQRCARWLLKSHDRVDGDELRLTQQFLAEMLVVSRVAVNAELQKFQQQQVLTYSRGIITVLDRKLLERTSCECYRAVRREYDRLLPGSFTK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-16" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSY2</accession>
    <name>RL10_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplJ</name>
        <name type="ordered locus">Nwi_1348</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the ribosomal stalk of the 50S ribosomal subunit. The N-terminus interacts with L11 and the large rRNA to form the base of the stalk. The C-terminus forms an elongated spine to which L12 dimers bind in a sequential fashion forming a multimeric L10(L12)X complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="ABA04609" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04609.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314627.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSY2"/>
    <dbReference type="SMR" id="Q3SSY2"/>
    <dbReference type="STRING" id="323098.Nwi_1348"/>
    <dbReference type="EnsemblBacteria" id="ABA04609">
        <property type="protein sequence ID" value="ABA04609"/>
        <property type="gene ID" value="Nwi_1348"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1348"/>
    <dbReference type="eggNOG" id="COG0244">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092227_0_0_5"/>
    <dbReference type="OrthoDB" id="1470978at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05797">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00362">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022973">
        <property type="entry name" value="Ribosomal_L10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_L10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002363">
        <property type="entry name" value="Ribosomal_L10_eubac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_L10P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11560">
        <property type="entry name" value="PTHR11560"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="SSF160369"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01109">
        <property type="entry name" value="RIBOSOMAL_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000234863" description="50S ribosomal protein L10">
        <location>
            <begin position="1"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="172" mass="17825" checksum="92E893C8F4E0F4FB" modified="2006-05-16" version="2">MERAAKKEAVEQLNETFRTTGVAIVAHYSGLTVAQMQTLRKQMKQAGASVKVSKNRLAKIALEGTDVVAIGSLLKGPTVIATSSDPVAAPKVAVEFAKTNENFVVLGGSMGKTVLDVNGVKALASLPSLDELRGKLVGLLVAPATKIAQLSTAPAAKLARVVQAYASKDEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSZ1</accession>
    <name>Q3SSZ1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">FecR protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1339</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314618.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1339"/>
    <dbReference type="EnsemblBacteria" id="ABA04600">
        <property type="protein sequence ID" value="ABA04600"/>
        <property type="gene ID" value="Nwi_1339"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1339"/>
    <dbReference type="eggNOG" id="COG3712">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050192_0_1_5"/>
    <dbReference type="OMA" id="IMVTSGH"/>
    <dbReference type="OrthoDB" id="1381035at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006860">
        <property type="entry name" value="FecR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032623">
        <property type="entry name" value="FecR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012373">
        <property type="entry name" value="Ferrdict_sens_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30273">
        <property type="entry name" value="PTHR30273"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16220">
        <property type="entry name" value="DUF4880"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04773">
        <property type="entry name" value="FecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018266">
        <property type="entry name" value="FecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4880" evidence="3">
        <location>
            <begin position="15"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="FecR" evidence="2">
        <location>
            <begin position="116"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="319" mass="34548" checksum="D6CB0FC699D4826F" modified="2005-10-11" version="1">MTADQSAQASRKRHEEAAAWVLKNRDINQSAHESRAFEEWLDRGEENRAAYEAAQRLMGEARTAILNDPALRNFKVKPRPRVAKTLAVAFLGAALAGSAFVYSDGPMRLLANAISGTGEMPTIELADGTTIQLNAFSAISYRFTAETRTVTLLRGQAFFQVAKDASRPFVVEANGGQTTALGTAFDIRLGQNSTEVTVTEHAVSVTPHTHAEPEVRVNEGQQAVYDADGKVKDVRQADTHAVLAWRRGQLVVDNAPVADVVAEIDRHFAGRIIISNSDLAARRVSGTFTVTDTDSALALLRESLGLSVIRMGPLILLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST17</accession>
    <name>Q3ST17_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PepSY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1313</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314594.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1313"/>
    <dbReference type="EnsemblBacteria" id="ABA04574">
        <property type="protein sequence ID" value="ABA04574"/>
        <property type="gene ID" value="Nwi_1313"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1313"/>
    <dbReference type="eggNOG" id="COG5591">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147864_2_0_5"/>
    <dbReference type="OMA" id="DWMPIDQ"/>
    <dbReference type="OrthoDB" id="2084476at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025711">
        <property type="entry name" value="PepSY"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13670">
        <property type="entry name" value="PepSY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229295" description="PepSY domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="PepSY" evidence="2">
        <location>
            <begin position="6"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="87" mass="9451" checksum="E594ACEE9E9EE6D0" modified="2005-10-11" version="1">MRIRYIALAMAATVAVAGLAKADQPGTDWMPADQVIEKLTAQGYTNFSKVEADDGHLELEADLKGVRYDLQVDPKSGEVTKSKPDND</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST29</accession>
    <name>Q3ST29_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1301</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314582.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04562">
        <property type="protein sequence ID" value="ABA04562"/>
        <property type="gene ID" value="Nwi_1301"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1301"/>
    <dbReference type="eggNOG" id="ENOG50345AJ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083874_0_0_5"/>
    <dbReference type="OMA" id="PRTEEDW"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="192" mass="21750" checksum="7F4CF0F892B38A08" modified="2005-10-11" version="1">METTTLTFAQIQARTGVNHSTITLWKRNHGWTRPAFAARHSYDVPLWRAGPRQKTAALTGRLVAIAERMVRDLEEKPDTDIDKLMQAMEALKMARLEARTRNRHWPVIGPSRTGREVLNEQEAIRAALKEMRRGGVDIDCAPQEALDLVIDANMPVEKDHPALRERGARRRGRQAAATTAMPTELSEIKLRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST57</accession>
    <name>Q3ST57_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acetyltransf_6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1273</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314562.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1273"/>
    <dbReference type="EnsemblBacteria" id="ABA04534">
        <property type="protein sequence ID" value="ABA04534"/>
        <property type="gene ID" value="Nwi_1273"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1273"/>
    <dbReference type="eggNOG" id="COG5653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051159_1_0_5"/>
    <dbReference type="OMA" id="YTWKTAY"/>
    <dbReference type="OrthoDB" id="1856290at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038740">
        <property type="entry name" value="BioF2-like_GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13480">
        <property type="entry name" value="Acetyltransf_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyltransf_6" evidence="1">
        <location>
            <begin position="203"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="406" mass="44462" checksum="21C19A42E6890919" modified="2005-10-11" version="1">MVDIANTAPARRSLMNEFVASPPRTLHRAAALASLDPDQWRSLADRAVEPNGYYLADWELALDATARGRTHASALTARSDFSSGETGAETLIGLIPAISAWRAYRIPLPLLVSADPYGTLCTPLLDRDRTDQAAATLLREARALGQRALILRNVTLDGAAVKAFTAALEPDGLQPHRLHGHARACLDATRDPEELLREALGAKKLKELRRQRNRLAEHGEVSFQVARAAAEVATALETFLALEASGWKAQRGTALIQHDGDAAFIRRAAPALAERGQCEIVTLRAGPTPVAAAIVLRHQDRAFYFKLGVDERFAKFSPGVQLTLDLTRHLCADPAITMADSTADPGHPMIDPIWRGRLVIGDVLIPLRRRDPVVSLARAAITLRKLVREPMRRAVHILRERQEKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST76</accession>
    <name>Q3ST76_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Site-specific DNA-methyltransferase (adenine-specific)</fullName>
            <ecNumber evidence="3">2.1.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_1254</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:15197"/>
            <dbReference type="Rhea" id="RHEA-COMP:12418"/>
            <dbReference type="Rhea" id="RHEA-COMP:12419"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90615"/>
            <dbReference type="ChEBI" id="CHEBI:90616"/>
            <dbReference type="EC" id="2.1.1.72"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the N(4)/N(6)-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.72" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314543.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1254"/>
    <dbReference type="REBASE" id="11319">
        <property type="entry name" value="M.NwiORF1254P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04515">
        <property type="protein sequence ID" value="ABA04515"/>
        <property type="gene ID" value="Nwi_1254"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1254"/>
    <dbReference type="eggNOG" id="COG0286">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018284_4_0_5"/>
    <dbReference type="OMA" id="THRLALM"/>
    <dbReference type="OrthoDB" id="791672at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009007">
        <property type="term" value="F:site-specific DNA-methyltransferase (adenine-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022749">
        <property type="entry name" value="D12N6_MeTrfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003356">
        <property type="entry name" value="DNA_methylase_A-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038333">
        <property type="entry name" value="T1MK-like_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12161">
        <property type="entry name" value="HsdM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02384">
        <property type="entry name" value="N6_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HsdM_N" evidence="5">
        <location>
            <begin position="5"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="N6_Mtase" evidence="4">
        <location>
            <begin position="142"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA04515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="484" mass="55025" checksum="1477CBBA1768A9A1" modified="2005-10-11" version="1">MIVRTTVKSIQDIMRQDVGVDGDAQRISQLTWLFFLKIIDDQDQELELTKDGYHSPIPKKLQWRTWAADPEGITGQALLDFVNDELFPALKGLQVSDRPGDRRRVVRDVFEDAYNYMKSGQLLRQVVNKITQVDFNNLDERRHFGEFYEQLLNDLQSAGNAGEYYTPRAVTAFMVQMIDPHPGEILFDPACGTGGFLSCAINHMEANYVRTPKQREKMQGGLRAVEKKQLPHMLCVTNMLLHGIEDPSFVKHDNTLARPLISWSKDERVDIVVTNPPFGGKEEDGIENNFPTFRTKETADLFLALIVRLLKPDGRAAVVLPDGTLFGEGVKTRLKEHLMEECNLHTIVRLPNSVFKPYASIGTNLLFFEKGAPTKDIWFYEHRVPEGQKAYSMTRPIRLEHFHGCIDWWGGKARKGRKETPQAWKVSAEEIKARGYNLDIKNPHAVADDHGDPETLLADLARAEAETATYRDQLKAILAEALLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STE9</accession>
    <name>Q3STE9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative phage cell wall peptidase, NlpC/P60</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1180</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C40 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314471.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1180"/>
    <dbReference type="EnsemblBacteria" id="ABA04442">
        <property type="protein sequence ID" value="ABA04442"/>
        <property type="gene ID" value="Nwi_1180"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1180"/>
    <dbReference type="eggNOG" id="COG0791">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115301_1_0_5"/>
    <dbReference type="OMA" id="PAYTPDW"/>
    <dbReference type="OrthoDB" id="682655at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000064">
        <property type="entry name" value="NLP_P60_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011929">
        <property type="entry name" value="Phage_pept_NlpC/P60"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00877">
        <property type="entry name" value="NLPC_P60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02219">
        <property type="entry name" value="phage_NlpC_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51935">
        <property type="entry name" value="NLPC_P60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NlpC/P60" evidence="2">
        <location>
            <begin position="5"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="148" mass="16024" checksum="4E858EFC72E20A62" modified="2005-10-11" version="1">MSCVCSTRAAIVAEARGWIGTPYRHQASLKGVGCDCLGLVRGVWRNCIGDEPETPPPYAPDWAEAKGDETLAAAALRHLVPVARDGFGAGDVLLFRWRDGFVAKHVAIASSEHAMVHAHDGAAVCEIAIAPWWRRRLAYAFRFPGVSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STH9</accession>
    <name>Q3STH9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidoglycan glycosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.129</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Nwi_1150</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = [GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23708"/>
            <dbReference type="Rhea" id="RHEA-COMP:9602"/>
            <dbReference type="Rhea" id="RHEA-COMP:9603"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:60033"/>
            <dbReference type="ChEBI" id="CHEBI:78435"/>
            <dbReference type="EC" id="2.4.1.129"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the transpeptidase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the glycosyltransferase 51 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.129" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314442.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1150"/>
    <dbReference type="CAZy" id="GT51">
        <property type="family name" value="Glycosyltransferase Family 51"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04412">
        <property type="protein sequence ID" value="ABA04412"/>
        <property type="gene ID" value="Nwi_1150"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1150"/>
    <dbReference type="eggNOG" id="COG0744">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006354_2_7_5"/>
    <dbReference type="OMA" id="VHGMGLA"/>
    <dbReference type="OrthoDB" id="652304at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008658">
        <property type="term" value="F:penicillin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008955">
        <property type="term" value="F:peptidoglycan glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3810.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001264">
        <property type="entry name" value="Glyco_trans_51"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036950">
        <property type="entry name" value="PBP_transglycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001460">
        <property type="entry name" value="PCN-bd_Tpept"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00912">
        <property type="entry name" value="Transgly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00905">
        <property type="entry name" value="Transpeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="7 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Transgly" evidence="13">
        <location>
            <begin position="117"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Transpeptidase" evidence="12">
        <location>
            <begin position="374"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="714"/>
            <end position="759"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABA04412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="759" mass="82802" checksum="B87FCCEC52080A6E" modified="2005-10-11" version="1">MRNILPPDWKKRIRHIMLDLDARIDSTLFSSAVGARELYERFSTFMDRFYVGHWKRWIFVEPLSEAATLGLGGLILLLALAQPAFRETADEDWLKKSDLAVTFLDRYGNPIGSRGIKHNDSIPLEEFPDNLIKATLATEDRRFYNHFGIDVGGTFRAVVTNAQAGGVRQGGSSITQQLAKNLFLSNERTIERKVNEAFLAVWLETRLSKNEILKLYLDRAYMGGGTFGVDGAAHFYFNKSVRDVNLAESAMLAGLFKAPTKYAPHINLPAARARANQVLDNMVDSGFMTEGQVFGARRNPATAVDRRDENSPNYYLDWAFEEMRRLVETFPRSYTERVFVVRTTIDMNVQHAAEEAIESQLRQFGRDYHATQAATVVADLDGGVRAMVGGRDYGASQFNRATDALRQPGSSFKPYVYTTALLNGFKPTSKIVDGPVCLGNWCPQNYGHSYSGTVTLTQALTRSINVVPVKLSIALGKGAKNPAKAGRAKIIEVARKFGIVTPLPDTPSLPIGADEVNVLEHAVAYATFPNKGKAVTPHAVLEVRTGAGDLVWRYDRDGPEPRQIIPPSVAADMAGMMSHVVSEGTARRAILDGIPAAGKTGTTNAYRDAWFVGYTGNFACGVWYGNDDYSPTNRLTGGALPAQTWHDIMTAAHRGIEIKDIPGVATPAKPTAAMTARTAASQDNATPAPPPILTRRGADILVRVEKMLDEAARTIGKTSAGEPVNPARSDATDPTSPRGEAKNVATASGDRAIPTPSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STJ6</accession>
    <name>Q3STJ6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Flagellar basal-body rod protein FlgC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_1133</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="5 6">The basal body constitutes a major portion of the flagellar organelle and consists of four rings (L,P,S, and M) mounted on a central rod. The rod consists of about 26 subunits of FlgG in the distal portion, and FlgB, FlgC and FlgF are thought to build up the proximal portion of the rod with about 6 subunits each.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Bacterial flagellum basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the flagella basal body rod proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314426.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1133"/>
    <dbReference type="EnsemblBacteria" id="ABA04395">
        <property type="protein sequence ID" value="ABA04395"/>
        <property type="gene ID" value="Nwi_1133"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1133"/>
    <dbReference type="eggNOG" id="COG1558">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123272_2_0_5"/>
    <dbReference type="OMA" id="YEANLRM"/>
    <dbReference type="OrthoDB" id="1870921at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030694">
        <property type="term" value="C:bacterial-type flagellum basal body, rod"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001444">
        <property type="entry name" value="Flag_bb_rod_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010930">
        <property type="entry name" value="Flg_bb/hook_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006299">
        <property type="entry name" value="FlgC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00460">
        <property type="entry name" value="Flg_bb_rod"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06429">
        <property type="entry name" value="Flg_bbr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01395">
        <property type="entry name" value="FlgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0969">Cilium</keyword>
    <keyword evidence="9" id="KW-0282">Flagellum</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flg_bb_rod" evidence="7">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Flg_bbr_C" evidence="8">
        <location>
            <begin position="93"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA04395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="141" mass="15410" checksum="343CA46DD15DA362" modified="2005-10-11" version="1">MADNGADFMRSMSIATSGLRAQAGRMRVISENIANANSTAQAPGGDPYRRKVPTFSSALDRALDAKVVTLGRVRPDTSTFRIKHEPGNPAADTSGNVKYPNVNPLVEMTDMRDAQRSYEANLNIISATRRMIQRTLDILKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STM9</accession>
    <name>Q3STM9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flagellar hook-associated protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_1100</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bacterial flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the flagella basal body rod proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314393.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1100"/>
    <dbReference type="EnsemblBacteria" id="ABA04362">
        <property type="protein sequence ID" value="ABA04362"/>
        <property type="gene ID" value="Nwi_1100"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1100"/>
    <dbReference type="eggNOG" id="COG1256">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012762_3_1_5"/>
    <dbReference type="OMA" id="KPQAAYM"/>
    <dbReference type="OrthoDB" id="1815809at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009424">
        <property type="term" value="C:bacterial-type flagellum hook"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044780">
        <property type="term" value="P:bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001444">
        <property type="entry name" value="Flag_bb_rod_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010930">
        <property type="entry name" value="Flg_bb/hook_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002371">
        <property type="entry name" value="FlgK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30033">
        <property type="entry name" value="PTHR30033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00460">
        <property type="entry name" value="Flg_bb_rod"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06429">
        <property type="entry name" value="Flg_bbr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01005">
        <property type="entry name" value="FLGHOOKAP1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02492">
        <property type="entry name" value="flgK_ends"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Flg_bb_rod" evidence="7">
        <location>
            <begin position="7"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="Flg_bbr_C" evidence="8">
        <location>
            <begin position="562"/>
            <end position="619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA04362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="622" mass="63645" checksum="EEF0D8341A42E395" modified="2005-10-11" version="1">MSLSSALSIAMSGLRANQAALSIVSGNVANANTPGYVAQTLVQDQVVTGGMGSGVRVIGVNRTLDAYVQSQLRTENAGGAYASHIAGVLGQLQNVYGTPGNDGTLEAAYNRFTSALQALSASSGNPAAQSSVLNAAQALAHQLNATTSGIQTLRSNAEQSLSASVSQANAAMQQIAQLNQRLQSMGAQDPAAATLMDQRDAAIDQLSGLMDIRAVTDGANQTSVFTTAGVQLVGGVYASTLTFNAQSSLTASSQWNADPAKSSVGTIICQLPNGARIDMIASQGINSGQIAADAQLRDKILVQAQNQVDQMAATLASALSDVTTSGAPVTGPPSGFDLDLSGILPGNAFRVTYTDSANVSYTVTVVRVDDPSALPIPNAAANPNDRVIGVDFSGGLASVVSQLNAQIGAASHLMFSASGSLLRVADDGSGQSTVSAASATTTVQSLASGDPQLPFFTDRASLYTGAITGSASQITGLAGRIQVNAALLADPSKLTVYSTSPVTPAGDTTRADFIYDRLTFASFSYAPNTGLGAAAAPFQGTLSSFLQQFVSLQSGAATTARQVAQGQSIVVNTLQEKFDDKSGVNMDTEMANLIALQNSYAANAHVMTVVQSMMQTLMQAQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STR0</accession>
    <name>Q3STR0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lipopolysaccharide heptosyltransferase II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1069</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314365.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1069"/>
    <dbReference type="CAZy" id="GT9">
        <property type="family name" value="Glycosyltransferase Family 9"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04331">
        <property type="protein sequence ID" value="ABA04331"/>
        <property type="gene ID" value="Nwi_1069"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1069"/>
    <dbReference type="eggNOG" id="COG0859">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038371_7_0_5"/>
    <dbReference type="OMA" id="GSAKRWP"/>
    <dbReference type="OrthoDB" id="1068323at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009103">
        <property type="term" value="P:lipopolysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03789">
        <property type="entry name" value="GT9_LPS_heptosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002201">
        <property type="entry name" value="Glyco_trans_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011910">
        <property type="entry name" value="RfaF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01075">
        <property type="entry name" value="Glyco_transf_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02195">
        <property type="entry name" value="heptsyl_trn_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="356" mass="38602" checksum="B68D209E53D9BB22" modified="2005-10-11" version="1">MNTDSSYEPEMVDPDDTRPILIIPYMWIGDFVRGHTVVRVLKQRWPNRPVDLLVTTLCAPLVDYMPGVRAGVVWDLPRSRLALAQQWGLARRLRARGYGTALVLPRTWKSAIAPTLAGIPERVGFVGEARFGLINRWRWGERALPRMIDRKASLALPAGAARPPEWPAPQLRVSGEDVARWRQAQGLGTGPAVALAPGAVGPSKRWSYFAEAARGLAERGIDIWVVGGPKEKALATDIVATGGPRVRDLTGHDLRNGILAMAGATIAISNDSGLMHVAAALGTPTMGIFGPTSPYHWGPLNGLAATIQAKTPLPCQPCHRPVCTRNDHRCMKDIPAAEVMAIAERVLAEAGMRDAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STR6</accession>
    <name>Q3STR6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Fumarylacetoacetate (FAA) hydrolase</fullName>
            <ecNumber evidence="2">4.1.1.68</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.1.1.68" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314359.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1063"/>
    <dbReference type="EnsemblBacteria" id="ABA04325">
        <property type="protein sequence ID" value="ABA04325"/>
        <property type="gene ID" value="Nwi_1063"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1063"/>
    <dbReference type="eggNOG" id="COG0179">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028458_5_1_5"/>
    <dbReference type="OMA" id="YALSIDM"/>
    <dbReference type="OrthoDB" id="775108at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018800">
        <property type="term" value="F:5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="SSF56529"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAA_hydrolase" evidence="1">
        <location>
            <begin position="29"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="234" mass="25321" checksum="590912E825F5098C" modified="2005-10-11" version="1">MSASPFVIPEPPRPVIAVAGGNGFFPVRRIWCVGRNYLDHIRELRNDERATPFFFAKHADMIEADGATIPYPPLTHDLHHEVELIVAMKSGSLRIPADTALDHVYGYAVGIDLTRRDLQNAARKKEQPWEIAKSFDHTAPCGALQPTAKIGHPSKGRIWLAVNGKETQSGDLAQMIWNVPEIIAQLSKQVSLDAGDIIMTGTPAGVSALHPGDHVECGCDGVGKLTITIGQPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STS0</accession>
    <name>Q3STS0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">UDP-3-O-acyl-N-acetylglucosamine deacetylase</fullName>
            <shortName evidence="12">UDP-3-O-acyl-GlcNAc deacetylase</shortName>
            <ecNumber evidence="4 12">3.5.1.108</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">UDP-3-O-[R-3-hydroxymyristoyl]-N-acetylglucosamine deacetylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">lpxC</name>
        <name evidence="13" type="ordered locus">Nwi_1059</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 12">Catalyzes the hydrolysis of UDP-3-O-myristoyl-N-acetylglucosamine to form UDP-3-O-myristoylglucosamine and acetate, the committed step in lipid A biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>a UDP-3-O-[(3R)-3-hydroxyacyl]-N-acetyl-alpha-D-glucosamine + H2O = a UDP-3-O-[(3R)-3-hydroxyacyl]-alpha-D-glucosamine + acetate</text>
            <dbReference type="Rhea" id="RHEA:67816"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:137740"/>
            <dbReference type="ChEBI" id="CHEBI:173225"/>
            <dbReference type="EC" id="3.5.1.108"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 2/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the LpxC family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.108" evidence="4 12"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314355.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1059"/>
    <dbReference type="EnsemblBacteria" id="ABA04321">
        <property type="protein sequence ID" value="ABA04321"/>
        <property type="gene ID" value="Nwi_1059"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1059"/>
    <dbReference type="eggNOG" id="COG0774">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046528_1_1_5"/>
    <dbReference type="OMA" id="IVFYRSD"/>
    <dbReference type="OrthoDB" id="428602at2"/>
    <dbReference type="UniPathway" id="UPA00359">
        <property type="reaction ID" value="UER00478"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008759">
        <property type="term" value="F:UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103117">
        <property type="term" value="F:UDP-3-O-acyl-N-acetylglucosamine deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009245">
        <property type="term" value="P:lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1700.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00388">
        <property type="entry name" value="LpxC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004463">
        <property type="entry name" value="UDP-acyl_GlcNac_deAcase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011334">
        <property type="entry name" value="UDP-acyl_GlcNac_deAcase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015870">
        <property type="entry name" value="UDP-acyl_N-AcGlcN_deAcase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33694">
        <property type="entry name" value="PTHR33694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03331">
        <property type="entry name" value="LpxC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00325">
        <property type="entry name" value="lpxC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 12" id="KW-0441">Lipid A biosynthesis</keyword>
    <keyword evidence="5 12" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10 12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton donor" evidence="12">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA04321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="318" mass="33840" checksum="B420498420A82FD8" modified="2005-10-11" version="1">MRFNRQTTLRSRATVTGVGVHSGLPANLTLGPASIDAGFVFIRTSPDGTERRVQAAAKSVTSTNFATVLGDQQGSLVSTAEHVLAALRGLGVDNATIELDGPEVPIMDGSSAAFVAAIDQAGIVEQSAARRFIQVLKPVRVTKGEAVGELRPYKRGFRVEVEIDFANPVIGHQDYSIDLDPESFRREISRARTFGCMNDVARLRSAGFALGASLENSMVFDDERLLNAGGLRYADECVRHKVLDAIGDLALAGLPLIGGYRSARGGHKLNHAVLTALMADRTAWRVVEDQPVSRPRSQGELVGGMVGGMAAAYGPNVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STX2</accession>
    <name>Q3STX2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Binding-protein-dependent transport systems inner membrane component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_1007</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314307.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1007"/>
    <dbReference type="EnsemblBacteria" id="ABA04269">
        <property type="protein sequence ID" value="ABA04269"/>
        <property type="gene ID" value="Nwi_1007"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1007"/>
    <dbReference type="eggNOG" id="COG1176">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_18_3_5"/>
    <dbReference type="OMA" id="YPIAYGM"/>
    <dbReference type="OrthoDB" id="1488093at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="86"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="215"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="86"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="304" mass="33470" checksum="4D168B385ABB735A" modified="2005-10-11" version="1">MSGRRLFSAPARRAAIAPFLWMLLFFLLPFGFVLKISLSHAVMAQPPYLPVFDPAEGIAAPKAAIAAFSLDNFRLLASDHFYVLSYLRSVVVALVSTSILLAVGYPIAYGMSRLPARWQPIAMILVIVPFWTSFLIRVYAWINILQHDGILNNVLMALRLISAPVVWLSTDAAMYIGLVYSYLPFMILPLYATLAKIDPALLEASADLGGSRLTTFWLVTVPLSLPGIGAGALLCFIPIVGEFVIPDLLAGSASPMIGQTLWLEFFNNHDWPVASSIAVVLLVLLLAPLLVYERVQRRRLEGTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU02</accession>
    <name>Q3SU02_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Uroporphyrinogen decarboxylase</fullName>
            <shortName evidence="8">UPD</shortName>
            <shortName evidence="8">URO-D</shortName>
            <ecNumber evidence="4 8">4.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hemE</name>
        <name evidence="10" type="ordered locus">Nwi_0977</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the decarboxylation of four acetate groups of uroporphyrinogen-III to yield coproporphyrinogen-III.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>4 H(+) + uroporphyrinogen III = 4 CO2 + coproporphyrinogen III</text>
            <dbReference type="Rhea" id="RHEA:19865"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57308"/>
            <dbReference type="ChEBI" id="CHEBI:57309"/>
            <dbReference type="EC" id="4.1.1.37"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 4/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the uroporphyrinogen decarboxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.37" evidence="4 8"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0977"/>
    <dbReference type="EnsemblBacteria" id="ABA04239">
        <property type="protein sequence ID" value="ABA04239"/>
        <property type="gene ID" value="Nwi_0977"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0977"/>
    <dbReference type="eggNOG" id="COG0407">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040933_0_0_5"/>
    <dbReference type="OMA" id="LWLMRQA"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00321"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004853">
        <property type="term" value="F:uroporphyrinogen decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00717">
        <property type="entry name" value="URO-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00218">
        <property type="entry name" value="URO_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006361">
        <property type="entry name" value="Uroporphyrinogen_deCO2ase_HemE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000257">
        <property type="entry name" value="Uroporphyrinogen_deCOase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01208">
        <property type="entry name" value="URO-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="SSF51726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01464">
        <property type="entry name" value="hemE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00907">
        <property type="entry name" value="UROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="6 8 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 8" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uroporphyrinogen_deCOase" evidence="9">
        <location>
            <begin position="117"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="3"/>
            <end position="7"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="8">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA04239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="325" mass="35489" checksum="070DFFA4CC6A4222" modified="2005-10-11" version="1">MMRQAGRYLPEYRELRAKAGGFLDLCFTPEFAAEVTLQPIRRFGFDAAIIFSDILVIPYALGRSVRFEAGEGPRLDPLDTPDKIAALSSEADMTKLEPVFEALRRVKSELDPKTALIGFCGAPWTVATYMVAGQGTPDQAPARMMAYRHPDAFAKVIDAIVDNSIRYLLGQLKAGADALQIFDTWAGVLPPREFAQWSIEPTKRIVAGVRRQVPDAKIIGFPRGAGALLPSYIEATGVDAVSIDWAAEPFLIRDKVQNRVAVQGNLDPLALIAGGAALNRAVDDVLTNFAGGRLIFNLGHGIQPETPIPHVEQMIRRVRGNGIRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU32</accession>
    <name>Q3SU32_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0947</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314250.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04209">
        <property type="protein sequence ID" value="ABA04209"/>
        <property type="gene ID" value="Nwi_0947"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0947"/>
    <dbReference type="HOGENOM" id="CLU_2437824_0_0_5"/>
    <dbReference type="OMA" id="MCITILN"/>
    <dbReference type="OrthoDB" id="2182684at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="73" mass="8112" checksum="6118EA79E499ACE4" modified="2005-10-11" version="1">MPIFSYFITDLAERKSKPIKVPLQNHEEAVMYGTLLAREMLGDTPELPDNGMCITILNDEGETVSILPFGSVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU51</accession>
    <name>Q3SU51_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">Ferrochelatase</fullName>
            <ecNumber evidence="7 8">4.99.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Heme synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Protoheme ferro-lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">hemH</name>
        <name evidence="9" type="ordered locus">Nwi_0928</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">Catalyzes the ferrous insertion into protoporphyrin IX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>2 H(+) + heme b = Fe(2+) + protoporphyrin IX</text>
            <dbReference type="Rhea" id="RHEA:22584"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:57306"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="EC" id="4.99.1.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Fe-coproporphyrin III + 2 H(+) = coproporphyrin III + Fe(2+)</text>
            <dbReference type="Rhea" id="RHEA:49572"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:68438"/>
            <dbReference type="ChEBI" id="CHEBI:131725"/>
            <dbReference type="EC" id="4.99.1.9"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="6">
            <dbReference type="Rhea" id="RHEA:49574"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="7 8">Porphyrin-containing compound metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the ferrochelatase family.</text>
    </comment>
    <dbReference type="EC" id="4.99.1.1" evidence="7 8"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0928"/>
    <dbReference type="EnsemblBacteria" id="ABA04190">
        <property type="protein sequence ID" value="ABA04190"/>
        <property type="gene ID" value="Nwi_0928"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0928"/>
    <dbReference type="eggNOG" id="COG0276">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018884_0_0_5"/>
    <dbReference type="OMA" id="LGDPYHC"/>
    <dbReference type="UniPathway" id="UPA00252">
        <property type="reaction ID" value="UER00325"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004325">
        <property type="term" value="F:ferrochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00419">
        <property type="entry name" value="Ferrochelatase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03411">
        <property type="entry name" value="Ferrochelatase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00323">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001015">
        <property type="entry name" value="Ferrochelatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019772">
        <property type="entry name" value="Ferrochelatase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033644">
        <property type="entry name" value="Ferrochelatase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033659">
        <property type="entry name" value="Ferrochelatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11108">
        <property type="entry name" value="PTHR11108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00762">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00109">
        <property type="entry name" value="hemH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00534">
        <property type="entry name" value="FERROCHELATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7 8" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="2 7 8" id="KW-0408">Iron</keyword>
    <keyword evidence="4 7 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA04190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="347" mass="38609" checksum="13A101186FFE41C1" modified="2005-10-11" version="1">MDMTVAIPIDKAGTAACAPRERVGVLLVNLGTPDTADARGVRVYLKEFLSDPRVIEKQSLGWKLVLNGIILNTRPRRKALDYLKIWNTELDESPLKTITRSQSDKLTAALEGHDHVVVDWAMRYGNPSMQSRIAAMAAQGCDRLLVVPLYPQYSATTSATVCDEAFRILSEMRAQPTLRVTPPYYDDPDYIDALAVSIETHLASLSFTPEIIMASFHGMPQAYVAKGDPYQGHCVATTEALRKRMGLDASKLMLTFQSRFGFDEWLQPYTDKTVEKLAKDGVKRLAVVTPGFSADCLETLEEIAQENAHIFRQNGGEEFTAIPCLNDSDPGMDVIRKLVLRELQGWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUC3</accession>
    <name>Q3SUC3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0854</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0854"/>
    <dbReference type="EnsemblBacteria" id="ABA04118">
        <property type="protein sequence ID" value="ABA04118"/>
        <property type="gene ID" value="Nwi_0854"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0854"/>
    <dbReference type="eggNOG" id="COG3843">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1061021_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021795">
        <property type="entry name" value="DUF3363"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11843">
        <property type="entry name" value="DUF3363"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="262" mass="29905" checksum="517D4385177D7678" modified="2005-10-11" version="1">MPVAGRRMASLDHAGMPVMMPRESDGEMRRDGSSAADVQPKHPVAKMFGPQTDDADVKDFTERCKDDRHHFRFIVSPEDASDMEDLKRFTRELMTQAEKDLGTKLEWVAVDHWNTDNPHVHVIVRGRADDGRDLVIDRDYIKSGMRDRAQDLITQELSPRTDLDISRALSRQIEAERWTQLDRQLVRDANEEGVIDVAPSPDRQPDAYLTQKVGRLRHLEHLGLAEAIGPGQWVMAEGAESTLRELGQRNDIIKRMHRALDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUE5</accession>
    <name>Q3SUE5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phage integrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0831</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314144.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0831"/>
    <dbReference type="EnsemblBacteria" id="ABA04096">
        <property type="protein sequence ID" value="ABA04096"/>
        <property type="gene ID" value="Nwi_0831"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0831"/>
    <dbReference type="eggNOG" id="COG0582">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027562_17_7_5"/>
    <dbReference type="OMA" id="TISYAYN"/>
    <dbReference type="OrthoDB" id="785253at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038488">
        <property type="entry name" value="Integrase_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025166">
        <property type="entry name" value="Integrase_DNA_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13356">
        <property type="entry name" value="Arm-DNA-bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyr recombinase" evidence="3">
        <location>
            <begin position="205"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="400" mass="45176" checksum="144063AFD4378F98" modified="2005-10-11" version="1">MKAHLTERNVKGLQPKQKNIIVYDEEVVGFGVRITSGGTRAFILTYRIEARERRLTIGAWPDWSVTAAREEAKRLKREIDQGRDPMAERDEARESPNVRQLIDRYLEEHASKLAKRNRDDQASMLRKLVEPAWGPRKAAEIQPDDVDRLLCQIAGGTLGKRGRKPTPVRANRVGEILRKMFNLAIRWRIRTDNPAAGFARNAEAPRDRYLSTDEIGRLSAALDAHPNRRAADAVRLILLTGARRGEVLGARWDQFDLDAAVWIKPAATTKQRRLHRAPISASAAALLRTIRLRVPKDCEWVFPGDAEGKPLQDIKRFWEDVRTKAELPAVRTHDLRHTFASLLVSGGMTLPMIGKLLGHTQVQTTQRYAHLLDDPLRAGLEQVGDMLRAKPRLVQNVATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUM3</accession>
    <name>Q3SUM3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Electron transfer flavoprotein beta-subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0753</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ETF beta-subunit/FixA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0753"/>
    <dbReference type="EnsemblBacteria" id="ABA04018">
        <property type="protein sequence ID" value="ABA04018"/>
        <property type="gene ID" value="Nwi_0753"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0753"/>
    <dbReference type="eggNOG" id="COG2086">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060196_0_0_5"/>
    <dbReference type="OMA" id="YNGGMVP"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01714">
        <property type="entry name" value="ETF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000049">
        <property type="entry name" value="ET-Flavoprotein_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012255">
        <property type="entry name" value="ETF_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033948">
        <property type="entry name" value="ETF_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294">
        <property type="entry name" value="PTHR21294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000090">
        <property type="entry name" value="Beta-ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01065">
        <property type="entry name" value="ETF_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ETF" evidence="3">
        <location>
            <begin position="25"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="251" mass="27002" checksum="1982A176DD1170A2" modified="2005-10-11" version="1">MSMKILVPVKRVVDFNVKIRLKGDGSGVELANVKMSMNPFDEIAVEEALRLQEAGKATEVVLVSIGPAEAAETIRTGLSMGADRGLLIQSEAPVEPLAVAKLLKAVVEEEGPGLIILGKQAIDDDSNQTGQMLAGLLGWAQATFASKIEVDEAHFNVTREVDGGLQTIRLKRPAIVTTDLRLNEPRYASLPNIMKAKKKPIVVRAANLYGVDLAPRLEILKTTEPPGRKAGVKVKDVAELVSRLRNEAGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUP4</accession>
    <name>Q3SUP4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH_37 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0731</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314051.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0731"/>
    <dbReference type="EnsemblBacteria" id="ABA03997">
        <property type="protein sequence ID" value="ABA03997"/>
        <property type="gene ID" value="Nwi_0731"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0731"/>
    <dbReference type="eggNOG" id="COG5606">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_163934_0_0_5"/>
    <dbReference type="OMA" id="ERKLMDC"/>
    <dbReference type="OrthoDB" id="1946648at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039554">
        <property type="entry name" value="HigA2-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13744">
        <property type="entry name" value="HTH_37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_37" evidence="1">
        <location>
            <begin position="17"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="111" mass="12424" checksum="51F9549ACAE6BDB4" modified="2005-10-11" version="1">MSIKNDDTELVHGSGNVFRDFGRDDADLLQAKALMASEIIKILDDRHWTTRKAEDATGISHADFSRIRRVNLGRFTLDRLMTILTLLGQEVEVAVSVRPRLPLGQPVPTNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUP7</accession>
    <name>Q3SUP7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0728</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314048.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0728"/>
    <dbReference type="EnsemblBacteria" id="ABA03994">
        <property type="protein sequence ID" value="ABA03994"/>
        <property type="gene ID" value="Nwi_0728"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0728"/>
    <dbReference type="eggNOG" id="COG0612">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039168_0_0_5"/>
    <dbReference type="OMA" id="CVWSRID"/>
    <dbReference type="OrthoDB" id="1516427at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="340"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="424" mass="49222" checksum="62403B491FF2E3A7" modified="2005-10-11" version="1">MILKASQRGSGQNLAAHLMRTDDNEHVLVHDLRGFASDDLHGAFKEAEAVSHGTKCRQYLFSLSLNPPEAENVPITVFEDAIERIEGKLKLEGQPRAIVFHEKEGRRHAHCVWSRIDAETMTARQMSFFKQKLMGVSRDLYLENGWKMPRGLENAAERNPTNFSLAEWQQAKRQDIDPRWLKTTLQHCWARSDNERSFVRSLEEHGFFLARGDRRSFVVLDHNGEVWSLARCLDLKSKEVAARLGDAAALPSVEVAKASVGARMTPALRRHVEESRARFQKRSATLGQRRMEMTHRQRAERAQLKATQETEWQRETRARAERLPKGLRGLWHRITGKYQQVRSENEREAQRTQERHTGERQTLIDRQRDERSLLQKQFRELRSQQAALLLELRKDIGRYLRMARARADSAALSRRRGSSLRLQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV20</accession>
    <name>Q3SV20_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphatidyl-N-methylethanolamine N-methyltransferase / phosphatidylethanolamine N-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.17</ecNumber>
            <ecNumber evidence="2">2.1.1.71</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0604</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.17" evidence="2"/>
    <dbReference type="EC" id="2.1.1.71" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313931.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0604"/>
    <dbReference type="EnsemblBacteria" id="ABA03871">
        <property type="protein sequence ID" value="ABA03871"/>
        <property type="gene ID" value="Nwi_0604"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0604"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_7_0_5"/>
    <dbReference type="OMA" id="RYWHIER"/>
    <dbReference type="OrthoDB" id="1865374at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080101">
        <property type="term" value="F:phosphatidyl-N-dimethylethanolamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000773">
        <property type="term" value="F:phosphatidyl-N-methylethanolamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004608">
        <property type="term" value="F:phosphatidylethanolamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="1">
        <location>
            <begin position="48"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="212" mass="23410" checksum="5A3688D28F17C230" modified="2005-10-11" version="1">MANDIDRAGVARAYGLWAPVYDLVFGKVFDPGRQSTIEIANAIGGRILDVGVGTGLSLSDYASTTKLYGVDLSEPMLRKAQQRVRSLNLTNVETLAVMDAKNLAFPDSFFDAVVAQFVITAVPDPEATLDDFVRVLKPGGELILVNHIGAEGGPRRVFELAFAPLARRLGWRPEFPWARLVNWAAKHGGVTLSERRLMRPLGHFSLIRYRKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV23</accession>
    <name>Q3SV23_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Basal-body rod modification protein FlgD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_0601</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">Required for flagellar hook formation. May act as a scaffolding protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the FlgD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313928.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0601"/>
    <dbReference type="EnsemblBacteria" id="ABA03868">
        <property type="protein sequence ID" value="ABA03868"/>
        <property type="gene ID" value="Nwi_0601"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0601"/>
    <dbReference type="eggNOG" id="COG1843">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047535_0_1_5"/>
    <dbReference type="OMA" id="STYAHVT"/>
    <dbReference type="OrthoDB" id="709873at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044781">
        <property type="term" value="P:bacterial-type flagellum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005648">
        <property type="entry name" value="FlgD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025965">
        <property type="entry name" value="FlgD_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025963">
        <property type="entry name" value="FLgD_Tudor"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03963">
        <property type="entry name" value="FlgD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13860">
        <property type="entry name" value="FlgD_ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13861">
        <property type="entry name" value="FLgD_tudor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="8" id="KW-0282">Flagellum</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FLgD_tudor" evidence="7">
        <location>
            <begin position="95"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="FlgD_ig" evidence="6">
        <location>
            <begin position="129"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA03868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="230" mass="23844" checksum="6EB169E233011C2A" modified="2005-10-11" version="1">MTTSVGSAVQQVVSGATAQPASSAASSATSSTGTLAGNFQTFLTLLTTQLQNQNPLDPLDTNQFTQQLVQFAGVEQQLKTNDQLSNLVSLQQTTQATQALDFVGKNAVIDGGTATMANNAVTWHLGIPTASTLTINIASSTGQNVFSGSYGVDAGDDQAFSWNGLGNDGTQWPDGQYTLTATAVDASGNPVGVSTQIKGQVNSVDLTQSPPLLSISGQTYTINQIRRIVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="132" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV29</accession>
    <name>Q3SV29_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Two component, sigma54 specific, transcriptional regulator, Fis family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_0595</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313923.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0595"/>
    <dbReference type="EnsemblBacteria" id="ABA03862">
        <property type="protein sequence ID" value="ABA03862"/>
        <property type="gene ID" value="Nwi_0595"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0595"/>
    <dbReference type="eggNOG" id="COG2204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_0_6_5"/>
    <dbReference type="OMA" id="ARYIHQQ"/>
    <dbReference type="OrthoDB" id="123059at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002197">
        <property type="entry name" value="HTH_Fis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002078">
        <property type="entry name" value="Sigma_54_int"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025943">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029995">
        <property type="entry name" value="TR_FlbD/FleR/FlgR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32071:SF21">
        <property type="entry name" value="PTHR32071:SF21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02954">
        <property type="entry name" value="HTH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00158">
        <property type="entry name" value="Sigma54_activat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01590">
        <property type="entry name" value="HTHFIS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00676">
        <property type="entry name" value="SIGMA54_INTERACT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50045">
        <property type="entry name" value="SIGMA54_INTERACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="9">
        <location>
            <begin position="2"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma-54 factor interaction" evidence="8">
        <location>
            <begin position="120"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="7">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA03862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="459" mass="49425" checksum="F58AB17D445C547B" modified="2005-10-11" version="1">MRLLIVGSLKGQLTTATKIAMDNGASVTHAESNEQAMAVVRGGKGADLLLVDVGLDIRDLVLRLEAEHIHVPIVACGIVSDARAAVAAIHAGAKEYIPLPPDPELIAAVLAAVANDSRDLVYRDEAMARVIKLAQQIAGSDASVMITGESGTGKEVLARHVHARSNRAKRPFISINCAAIPEHLLESELFGHEKGAFTGAIARRIGKFEEASGGTLLLDEISEMDVRLQSKLLRAIQERVIDRVGGARPVPVDIRIIATSNRNLADAVRQGTFREDLLFRLNVVNLKIPPLRERPADIIELAQHFIRKYAAANGVPARPLSADARRVLSANRWQGNVRELENTIHRAVLMANGDEIGADAILTPEGDRLDLIRTAPAVAHATLAAEQVTRALVGRTVANVERDLILETLKHCLGNRTHAANILGISIRTLRNKLNEYADDGIAITPAGNSDHRAFIATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV52</accession>
    <name>Q3SV52_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Methylated-DNA-(Protein)-cysteine S-methyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.63</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0572</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 4-O-methyl-thymidine in DNA + L-cysteinyl-[protein] = a thymidine in DNA + S-methyl-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:53428"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:13555"/>
            <dbReference type="Rhea" id="RHEA-COMP:13556"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:137386"/>
            <dbReference type="ChEBI" id="CHEBI:137387"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 6-O-methyl-2'-deoxyguanosine in DNA + L-cysteinyl-[protein] = a 2'-deoxyguanosine in DNA + S-methyl-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:24000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:11367"/>
            <dbReference type="Rhea" id="RHEA-COMP:11368"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:85445"/>
            <dbReference type="ChEBI" id="CHEBI:85448"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.63" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0572"/>
    <dbReference type="EnsemblBacteria" id="ABA03839">
        <property type="protein sequence ID" value="ABA03839"/>
        <property type="gene ID" value="Nwi_0572"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0572"/>
    <dbReference type="eggNOG" id="COG0350">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2667395_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003908">
        <property type="term" value="F:methylated-DNA-[protein]-cysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06445">
        <property type="entry name" value="ATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001497">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014048">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036217">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNAb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01035">
        <property type="entry name" value="DNA_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46767">
        <property type="entry name" value="SSF46767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00589">
        <property type="entry name" value="ogt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00374">
        <property type="entry name" value="MGMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA_binding_1" evidence="6">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="75" mass="7817" checksum="EA30F601D78D4FAF" modified="2005-10-11" version="1">MTYGDIAKRLGGVELSRDVGQALGRNPCPIVVPCHRVLAAGDRPGGFSASDGVTTKLRMLAIEGAIVNHTPGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV57</accession>
    <name>Q3SV57_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Peptidase M23B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0567</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313895.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0567"/>
    <dbReference type="EnsemblBacteria" id="ABA03834">
        <property type="protein sequence ID" value="ABA03834"/>
        <property type="gene ID" value="Nwi_0567"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0567"/>
    <dbReference type="eggNOG" id="COG0739">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026846_2_0_5"/>
    <dbReference type="OMA" id="YVRMHTG"/>
    <dbReference type="OrthoDB" id="1891666at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016047">
        <property type="entry name" value="Peptidase_M23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01551">
        <property type="entry name" value="Peptidase_M23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51261">
        <property type="entry name" value="SSF51261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M23" evidence="3">
        <location>
            <begin position="539"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="692" mass="74051" checksum="D1544D32E4AA58F7" modified="2005-10-11" version="1">MTIAGFWGTTDLNTARAGHKGRETGLIDLGHEPPLSADGSEAAVIDRRRVSVQWFSGTILTGLCGAALIGGAVFASLDGETTFAQVAERVEGGLRGSFGTGDHQASLHKGDRLPPPSEATAARQVIRVSTVTRAGNRDVMRVRPFVRVSGNLSMATGDLSARVPPFNAQRLLADVGGTTRTTSEDASAAAAAEPDAEVSFVTRDLASILPKAKIAAILPIDEVLMRVRDASNWRGNSGVRYTMADAATDAPGATSSIKMAYAAEGGVDPYAGFEARIVPENVTLLPKTSEQLTGGNPIGERVHLVRRGDTIVSVLRDQGATPDEARAIATTLGPRGRDGGLREGQKLRIMMAPATSDQRLVPYRVIVANDSVIEAVAALSDLGNYVAVDVASMNSVADVADSSDNDDGGGARLYQSIYETALRDKVPPAVIEDMIRIYSYDIDFQRKVQPGDSFDVFYAGDDEGTPTTEKNEVLYASLTVGGETKKYFRFQTPDDAMVDYYDETGKSAKKFLVRKPAASAVMRSGFGTRRHPILGYVKMHTGVDWAASYGTPIFAAGNGVVEKAAWEGGYGKYVRIKHNNGYETAYGHMSAFAKGMEPGKRVRQGQVIGFVGSTGRSTGAHVHYEILVNGRFVDPMRVRLPRGRSLNGTILASFEKERDRLDAMMNSRNTVARVSDAAGSPALPLPRQVSNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVB8</accession>
    <name>Q3SVB8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">Phosphate-binding protein PstS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_0506</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Part of the ABC transporter complex PstSACB involved in phosphate import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 6">The complex is composed of two ATP-binding proteins (PstB), two transmembrane proteins (PstC and PstA) and a solute-binding protein (PstS).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the PstS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313834.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0506"/>
    <dbReference type="EnsemblBacteria" id="ABA03773">
        <property type="protein sequence ID" value="ABA03773"/>
        <property type="gene ID" value="Nwi_0506"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0506"/>
    <dbReference type="eggNOG" id="COG0226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034528_1_0_5"/>
    <dbReference type="OMA" id="FPYPVYA"/>
    <dbReference type="OrthoDB" id="1222928at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042301">
        <property type="term" value="F:phosphate ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005673">
        <property type="entry name" value="ABC_phos-bd_PstS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024370">
        <property type="entry name" value="PBP_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12849">
        <property type="entry name" value="PBP_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002756">
        <property type="entry name" value="PstS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00975">
        <property type="entry name" value="3a0107s03"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229282" description="Phosphate-binding protein PstS" evidence="7">
        <location>
            <begin position="27"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="PBP_domain" evidence="8">
        <location>
            <begin position="27"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA03773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="341" mass="36002" checksum="933EF860E72CCED0" modified="2005-10-11" version="1">MNFFKAIAAAGLVAASASVFSVPVKAADVTGAGATFPFPVYSKWADAYKKETGHGVNYQSIGSGAGIKQILAKTVTFGATDAPLKADKLEKDGLAQWPMVMGAIVPVVNIEGVKPGELVLSGELLGDIYLGKVTKWDDPAIVKLNPSVKLPSASIAVVHRSDGAGTSFNFTNYLSKVNGEWKSKVGEGTAVEWPAGVGAKGNEGVSGNVGQTRNAIGYVEYAYAKQNKLTYTALINKAGKVVQPGVASFQAAAANADWASAPGYHVILTDQPGEASWPITAATFILMYREPSDKAASAEAIKFFKWAFEKGGVMAEELDYIPMPESVVKQIEKTWTSEIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVC2</accession>
    <name>Q3SVC2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">OmpA/MotB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0502</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313830.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0502"/>
    <dbReference type="EnsemblBacteria" id="ABA03769">
        <property type="protein sequence ID" value="ABA03769"/>
        <property type="gene ID" value="Nwi_0502"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0502"/>
    <dbReference type="eggNOG" id="COG2885">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024178_0_0_5"/>
    <dbReference type="OMA" id="FDACSAD"/>
    <dbReference type="OrthoDB" id="903581at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07185">
        <property type="entry name" value="OmpA_C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006664">
        <property type="entry name" value="OMP_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006665">
        <property type="entry name" value="OmpA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036737">
        <property type="entry name" value="OmpA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00691">
        <property type="entry name" value="OmpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01021">
        <property type="entry name" value="OMPADOMAIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103088">
        <property type="entry name" value="SSF103088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51123">
        <property type="entry name" value="OMPA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OmpA-like" evidence="4">
        <location>
            <begin position="348"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="432"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="463" mass="49637" checksum="66A9E0522AE50303" modified="2005-10-11" version="1">MRGFFRWSSKWWPGVIPLTVLWVLAAWTTTAPLEADLSEQTAVALKDSVLDKTAISAAGRDITFSADAFSEQERSNAVASVETVPGVRRVNDRTALVAEAKPFVWTIERGVGRVTLGGSAPLPVVRNKLLDAARARFAGVDIADRMDLKRGAPPRFEAAAILLIGETSKLKNGKATISDLTVNLSGMARELGGREAIAAALKNLPEGYSVADDIQAPPYVFEANKDPVSGVLTLTGYVPDNAVHAAIVASAGRKFGEKIVDKLKASLGAPAGFDAVVTRALRSLSRLSTGRLVVSDRNVELSGDALYDLAGKQIRADLPKDLPRGWETKTDVSIKPSASPVDPTVCQQLFGELLEKRTIQFEAGQATLHLDSIGLLDQLTETALRCQTATIEIVGHTDSDGDASFNQTLSERRAQAVVDFLVKAGLPANRFTPMGRGSAQPIATNDTDEGKAKNRRIDFIVRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="131" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVG0</accession>
    <name>Q3SVG0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Dihydroorotate dehydrogenase (quinone)</fullName>
            <ecNumber evidence="11">1.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">DHOdehase</fullName>
            <shortName evidence="11">DHOD</shortName>
            <shortName evidence="11">DHODase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Dihydroorotate oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">pyrD</name>
        <name evidence="13" type="ordered locus">Nwi_0464</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 11">Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>(S)-dihydroorotate + a quinone = a quinol + orotate</text>
            <dbReference type="Rhea" id="RHEA:30187"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="11">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 11">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 11">Cell membrane</location>
            <topology evidence="3 11">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 11">Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.2" evidence="11"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313795.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0464"/>
    <dbReference type="EnsemblBacteria" id="ABA03731">
        <property type="protein sequence ID" value="ABA03731"/>
        <property type="gene ID" value="Nwi_0464"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0464"/>
    <dbReference type="eggNOG" id="COG0167">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013640_2_1_5"/>
    <dbReference type="OMA" id="ERIKMGA"/>
    <dbReference type="OrthoDB" id="1109542at2"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00946"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004152">
        <property type="term" value="F:dihydroorotate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04738">
        <property type="entry name" value="DHOD_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00225">
        <property type="entry name" value="DHO_dh_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005720">
        <property type="entry name" value="Dihydroorotate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005719">
        <property type="entry name" value="Dihydroorotate_DH_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001295">
        <property type="entry name" value="Dihydroorotate_DH_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01180">
        <property type="entry name" value="DHO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01036">
        <property type="entry name" value="pyrD_sub2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00911">
        <property type="entry name" value="DHODEHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00912">
        <property type="entry name" value="DHODEHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7 11" id="KW-0288">FMN</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 11" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHO_dh" evidence="12">
        <location>
            <begin position="44"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="11">
        <location>
            <begin position="110"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="11">
        <location>
            <begin position="243"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="61"/>
            <end position="65"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="316"/>
            <end position="317"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA03731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="364" mass="39203" checksum="1E0C6BE8E74E73EA" modified="2005-10-11" version="1">MIRAFDNLSLPLLRWLDPEDAHRMAIQGLRLLPPIRQRADDPKLAARAFGLNFPNPIGLAAGFDKSAEVPDALLKLGFGFVEIGSVTPRAQPGNPRPRVFRLERDEAIINRMGFNNDGAHTVLRRLAARAHSGGIVGVNIGANKDSANRIADYVRLIEIFAPVASYFTVNVSSPNTPGLRNLQQAAALDELLAKVIDARERVRQNAGDTPVLLKIAPDLTLGELDDVVHTARSRRIDGMIVGNTTLARPSTLRDQSRAKEQGGLSGRPLFQLSTRMVAETYVRAEGAFPLIGVGGIDSGGAALTKIRAGATLIQLYSSLVYKGLGLVDAIKRDLTSTLLRTGRDSLSEIIGADAATITAEDWPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVG4</accession>
    <name>Q3SVG4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carboxymuconolactone decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313791.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0460"/>
    <dbReference type="EnsemblBacteria" id="ABA03727">
        <property type="protein sequence ID" value="ABA03727"/>
        <property type="gene ID" value="Nwi_0460"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0460"/>
    <dbReference type="eggNOG" id="COG2128">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082760_5_1_5"/>
    <dbReference type="OMA" id="HTGIAKM"/>
    <dbReference type="OrthoDB" id="1925754at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004675">
        <property type="entry name" value="AhpD_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="SSF69118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00778">
        <property type="entry name" value="ahpD_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMD" evidence="1">
        <location>
            <begin position="52"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="190" mass="19976" checksum="296983BD7E8D2484" modified="2005-10-11" version="1">MTRLPVHTAVTAPEAARERVQNAEKANGFLPNLIGLLANAPTALETYQTVSGINARAGLTLAEREAVQITAAITHGCGFCVAGHTAIAYKKADLKDAAVNALRDGVHGPDARLNAVADFTRAVIRSRGRVEDAELNAFRAAGFSDANALEVVLGVSLATLCNFANNLGQTDINPELKPYAWNNPTTVAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVH2</accession>
    <name>Q3SVH2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Peptidase M48, Ste24p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_0452</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313783.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0452"/>
    <dbReference type="EnsemblBacteria" id="ABA03719">
        <property type="protein sequence ID" value="ABA03719"/>
        <property type="gene ID" value="Nwi_0452"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0452"/>
    <dbReference type="eggNOG" id="COG4784">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029002_5_1_5"/>
    <dbReference type="OMA" id="WMENVDK"/>
    <dbReference type="OrthoDB" id="1445800at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase_M48" evidence="8">
        <location>
            <begin position="82"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="238"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="245"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA03719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="496" mass="53937" checksum="3950CF393C9F34B7" modified="2005-10-11" version="1">MTASPVWRGTEAIRLSAAPVMLLCATLALGGCGDITRFQTASPSSAKSVKPARAAAPTPKSEHEHKRILASYGGAYDDPKLEALINRTVERLVAVSERPDLTYKVTILNSGAVNAFALPTGQLYVTRGLVALANDTSELSSVLSHEMAHVLAKHATIREDQARQAALVSHVVTEMGTDPDLTALALAKSKLTLASFSRAQEFEADGIGVGISARAHFDPFGAARFLAAMERNAALKAGKPSLDPRSQDFLSSHPATPERVQNAQANARQYSAPAQGERDRETYLNAIDNIVYGEDPSEGFVRGRRFLHPKLGFTFQAPEAFTLDNTAQAVIGIREGGSQAMRFDVVRVPAEQKLTEYLNSGWMENVNKASTEELTINGFPAASAVASGEEWQFRVYALRFGSDVYRFIFAARRKTTESERNARETVASFRRLTLGEIQAARPLRIKVITVQPGDTVESLSHRMAGVDHPAERFRVLNGLDPRAQVKVGNRVKIVVD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVH3</accession>
    <name>RPPH_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA pyrophosphohydrolase</fullName>
            <ecNumber evidence="1">3.6.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">(Di)nucleoside polyphosphate hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rppH</name>
        <name evidence="1" type="synonym">nudH</name>
        <name type="ordered locus">Nwi_0451</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Accelerates the degradation of transcripts by removing pyrophosphate from the 5'-end of triphosphorylated RNA, leading to a more labile monophosphorylated state that can stimulate subsequent ribonuclease cleavage.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Nudix hydrolase family. RppH subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313782.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVH3"/>
    <dbReference type="SMR" id="Q3SVH3"/>
    <dbReference type="STRING" id="323098.Nwi_0451"/>
    <dbReference type="PRIDE" id="Q3SVH3"/>
    <dbReference type="EnsemblBacteria" id="ABA03718">
        <property type="protein sequence ID" value="ABA03718"/>
        <property type="gene ID" value="Nwi_0451"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0451"/>
    <dbReference type="eggNOG" id="COG1051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087195_3_0_5"/>
    <dbReference type="OMA" id="PCVGIML"/>
    <dbReference type="OrthoDB" id="1345242at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016462">
        <property type="term" value="F:pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd03671">
        <property type="entry name" value="Ap4A_hydrolase_plant_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00298">
        <property type="entry name" value="Nudix_RppH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022927">
        <property type="entry name" value="RppH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000231918" description="RNA pyrophosphohydrolase">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Nudix hydrolase" evidence="1">
        <location>
            <begin position="8"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Nudix box">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00298"/>
        </source>
    </evidence>
    <sequence length="170" mass="19584" checksum="2DAD25E489A527EE" modified="2005-10-11" version="1">MTRYDDLPYRTCVGMMLINERGLVFVGRRAGVEQVDDSYVWQMPQGGVDPGEDTWLAAKRELYEETSVRSIEKLAEIPDWLTYDIPRVVAGRAWKGRYRGQRQKWYAVRFIGEDNEINIANPGDGHKPEFTAWRWEPMQNLTGLIIPFKRPVYERVVKEFASLAGAQAGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVM7</accession>
    <name>Q3SVM7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Mg2+ and Co2+ transporter CorB family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Nwi_0397</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0053 family. Hemolysin C subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313728.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0397"/>
    <dbReference type="EnsemblBacteria" id="ABA03664">
        <property type="protein sequence ID" value="ABA03664"/>
        <property type="gene ID" value="Nwi_0397"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0397"/>
    <dbReference type="eggNOG" id="COG1253">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015237_4_1_5"/>
    <dbReference type="OMA" id="GVAHIKD"/>
    <dbReference type="OrthoDB" id="1155802at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04590">
        <property type="entry name" value="CBS_pair_CorC_HlyC_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002550">
        <property type="entry name" value="CNNM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044751">
        <property type="entry name" value="Ion_transp-like_CBS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005170">
        <property type="entry name" value="Transptr-assoc_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03471">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01595">
        <property type="entry name" value="DUF21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01091">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51846">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0129">CBS domain</keyword>
    <keyword evidence="7 9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="CNNM transmembrane" evidence="12">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="11">
        <location>
            <begin position="220"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="11">
        <location>
            <begin position="287"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA03664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="449" mass="49840" checksum="9D0685EDF9964CBE" modified="2005-10-11" version="1">MSSTLQILLAVLLLAANGFFVAAEFALVKSRGYRVKAMVERGQFGARLMRDIMGNIEAYLACCQLGITMASLGLGWVGEPTVEALLAPLLRPLGMPEPALHLTAFLTGFLVFSSLHIVIGEQVPKTLAIRQPEPVSQWIIYPLHASYVLFYPLNWLLNSASRAILRALRVKEVSQHDVLTGAEIEGLIGESAEHGGIESGEAEYIQNVFRFGDLAVSDVMVHRTAMVTINADLPPEDLVREVLASEYTRIPLWRDKPENIVGVLHAKDLLRAIHASEGDIARINVNAIALPPWFVPEMRPVSDQLKAFRRRKTHFALVVDEYGEVEGMVTLEDVLEEIVGDISDEHDVVVAGVRQQADGSVVVDGSVPIRDLNRAMDWHLPDEEATTIAGLVIHEARSIPERGQNFTFYGFRFRVLRRERNRITALRIQPVPRGGEAIENRPKRTGMLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVP1</accession>
    <name>Q3SVP1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0383</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313714.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0383"/>
    <dbReference type="EnsemblBacteria" id="ABA03650">
        <property type="protein sequence ID" value="ABA03650"/>
        <property type="gene ID" value="Nwi_0383"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0383"/>
    <dbReference type="eggNOG" id="COG5319">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_191430_0_0_5"/>
    <dbReference type="OMA" id="CANEHVA"/>
    <dbReference type="OrthoDB" id="1914830at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009562">
        <property type="entry name" value="DUF1178"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06676">
        <property type="entry name" value="DUF1178"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="81" mass="8962" checksum="CF1CA25B33E64097" modified="2005-10-11" version="1">MIRYNLRCEQGHTFESWFQSSSSYDSQVKRKLVTCAVCGSTKVEKAIMAPQIAGSRKRRTPRLAAAPAFTIDEPPERGGSA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVS4</accession>
    <name>PCKA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoenolpyruvate carboxykinase (ATP)</fullName>
            <shortName evidence="1">PCK</shortName>
            <shortName evidence="1">PEP carboxykinase</shortName>
            <shortName evidence="1">PEPCK</shortName>
            <ecNumber evidence="1">4.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pckA</name>
        <name type="ordered locus">Nwi_0350</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) through direct phosphoryl transfer between the nucleoside triphosphate and OAA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + oxaloacetate = ADP + CO2 + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:18617"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.1.1.49"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 1 Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphoenolpyruvate carboxykinase (ATP) family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.49" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313682.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVS4"/>
    <dbReference type="SMR" id="Q3SVS4"/>
    <dbReference type="STRING" id="323098.Nwi_0350"/>
    <dbReference type="EnsemblBacteria" id="ABA03617">
        <property type="protein sequence ID" value="ABA03617"/>
        <property type="gene ID" value="Nwi_0350"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0350"/>
    <dbReference type="eggNOG" id="COG1866">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018247_0_1_5"/>
    <dbReference type="OMA" id="MRYAGEM"/>
    <dbReference type="OrthoDB" id="146648at2"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004612">
        <property type="term" value="F:phosphoenolpyruvate carboxykinase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00484">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.449.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00453">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001272">
        <property type="entry name" value="PEP_carboxykinase_ATP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013035">
        <property type="entry name" value="PEP_carboxykinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008210">
        <property type="entry name" value="PEP_carboxykinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015994">
        <property type="entry name" value="PEPCK_ATP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30031">
        <property type="entry name" value="PTHR30031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01293">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006294">
        <property type="entry name" value="PEP_crbxkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68923">
        <property type="entry name" value="SSF68923"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00224">
        <property type="entry name" value="pckA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00532">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0210">Decarboxylase</keyword>
    <keyword id="KW-0312">Gluconeogenesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000236930" description="Phosphoenolpyruvate carboxykinase (ATP)">
        <location>
            <begin position="1"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="236"/>
            <end position="244"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="449"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00453"/>
        </source>
    </evidence>
    <sequence length="538" mass="58963" checksum="21CBAE9E90F285EC" modified="2005-10-11" version="1">MPEIGVRNGAFGADKFGLQNLKAVYWNPGAPQLYEHALRSGEAMVNADGALCAETGIFTGRSPKDKFTVRDAATDKSVWWAGNQSMTPDQFAALYSDFLAHARNMTLFAQDLYGGADPNFRIKTRVFTELAWHSLFIRTLLRRPETSELAAFVPELTVIDLPSFRADPQRHGVRSENVVAIDFTRRIILIGGSHYAGEMKKSIFTTLNYYLPDQDVLPMHCSANVGPGGDSAIFFGLSGTGKTTLSADPGRTLIGDDEHGWSKDGIFNFEGGCYAKCIRLSREAEPEIYAASKRFGAVLENVVHDEVTRVPDFNDGSKTENTRSAYPLEFIPNASLTGCAGQPKNLVMLAADAFGVLPPIARLTPAQAMYHFLSGYTAKVAGTERDLGNEPQPEFSACFGSPFLPRHPGVYGNMLRALIAKHNVDCWLVNTGWTGGKYGTGRRMPIKVTRALLGAALDGSLRGVPFHDDRYFGFAVPTSVPGVEPHILDPIRTWADKIEFDRTARALIGMFRQNFTKFENDVDAEVREAAPGTMMAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVU5</accession>
    <name>Q3SVU5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Porin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0329</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell outer membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the OprB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0329"/>
    <dbReference type="EnsemblBacteria" id="ABA03596">
        <property type="protein sequence ID" value="ABA03596"/>
        <property type="gene ID" value="Nwi_0329"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0329"/>
    <dbReference type="eggNOG" id="COG3637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3659">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_392228_0_0_5"/>
    <dbReference type="OMA" id="SRDHRDF"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007049">
        <property type="entry name" value="Carb-sel_porin_OprB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027385">
        <property type="entry name" value="OMP_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038673">
        <property type="entry name" value="OprB_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13505">
        <property type="entry name" value="OMP_b-brl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04966">
        <property type="entry name" value="OprB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="2" id="KW-0406">Ion transport</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0626">Porin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007232144" description="Porin" evidence="2">
        <location>
            <begin position="42"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="domain" description="OMP_b-brl" evidence="3">
        <location>
            <begin position="50"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="674" mass="73396" checksum="77E7351235D50A39" modified="2005-10-11" version="1">MKGIRSNRPRQIQPAQALKRATCALAGLAASLLAQNGFAQAADFPAVRKARPLTAIDWSGFYVGGHAGFGGGRSAATLFDPAPAAGRNVFGGLIGGIQAGYNVRLPSGLLLGLEADVSFPSYIESNSFVSGLATARSDITHQWDFIGTARARFGYAAGPWLAYATGGLAFAGDRYLNDLPSGDQEKMLDARFGWVAGAGLEYAFAPYWSARLEYLHYQFEGTGVQFPSAAQYAATMNFQSIRVGLNRRLGGSLPGTGPAEPPAGLESDRWEVHGQTVYLGQGYPSFRAPYSGPNSLSPAAQHQATWSNALFLNARLWDGGEVYYNPELLQGFGLSDTVGVAGFPSGEAQKSNFPYPRYNTSRLFVRQTFGFGGEQEDLASDPGQIAGKADISRLTLQAGKMAVIDMFDGNVYAKDTRADFMNWSLWAPGAFDYAADRLGLTYGVTAELNQKQWALRGGYFLMNSESNADSFDTQVFRRGEYVVELETRFSLFGRPGRLRSLGWLNSAFMGSYREALNDPANLDISRTRMGRIKYGYAINVEQAVTDDIGLFGRWSWNDGRTEIMAFTDIDASLSLGTSIKGTGWGRPEDTIGIGGAINALSRDHRDFIAAGGLSVLIGDGRLNYRRERILETYYAFALNRWVTLTADYQFITNPGYNADRGPVHVFSGRLRGEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVX1</accession>
    <name>Q3SVX1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Peptidase A24A, prepilin type IV</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0303</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313636.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0303"/>
    <dbReference type="EnsemblBacteria" id="ABA03570">
        <property type="protein sequence ID" value="ABA03570"/>
        <property type="gene ID" value="Nwi_0303"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0303"/>
    <dbReference type="eggNOG" id="COG4960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057101_9_0_5"/>
    <dbReference type="OMA" id="DLFTMTI"/>
    <dbReference type="OrthoDB" id="2046608at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000045">
        <property type="entry name" value="Prepilin_IV_endopep_pep"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01478">
        <property type="entry name" value="Peptidase_A24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="141"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_A24" evidence="3">
        <location>
            <begin position="10"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="174" mass="18539" checksum="66A1AA77807AD37C" modified="2005-10-11" version="1">MVLDVARLLLFPALMAFAAASDLLTMTISNRVSLLLVAGFLVLAPLSGMGLHEMLSHAGAGLAVLVVAFSCFAMGWVGGGDAKVAAAAALWFGFGHTLEYLVYASLFGGALTLLLLQFRQWPLPASLYGQDWLLRLHGKNTGIPYGIALAFAALLIYPETDWVRAVDLARFGLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVY7</accession>
    <name>Q3SVY7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cytochrome c, class I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0287</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313620.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0287"/>
    <dbReference type="EnsemblBacteria" id="ABA03554">
        <property type="protein sequence ID" value="ABA03554"/>
        <property type="gene ID" value="Nwi_0287"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0287"/>
    <dbReference type="eggNOG" id="COG3474">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060944_4_0_5"/>
    <dbReference type="OMA" id="KGYIPGT"/>
    <dbReference type="OrthoDB" id="1939800at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002327">
        <property type="entry name" value="Cyt_c_1A/1B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11961">
        <property type="entry name" value="PTHR11961"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00034">
        <property type="entry name" value="Cytochrom_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00604">
        <property type="entry name" value="CYTCHRMECIAB"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cytochrome c" evidence="6">
        <location>
            <begin position="82"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="185" mass="19368" checksum="9471F9A44C73C01E" modified="2005-10-11" version="1">MINKFIGAVLGTCLVLLVMNLIARAVFAPAVPAKPGYKIDVKEEPAAVQKDDKPVAASKAAAASKEADVAAAVSLGKRLQAASAENGAVVAKKCAACHTFEKGGPNRVGPNLYGIVGEPRGQGHDFNFSAAMKAKGGTWTLDELDKFLANPKEYIPGTAMSFVGVPKDSDRADLIAYLRKNSDKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="127" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVZ1</accession>
    <name>Q3SVZ1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 20">Methionine synthase</fullName>
            <ecNumber evidence="6 20">2.1.1.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19 20">5-methyltetrahydrofolate--homocysteine methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="30" type="ordered locus">Nwi_0283</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="31">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18 20">Catalyzes the transfer of a methyl group from methyl-cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 20">
            <text>(6S)-5-methyl-5,6,7,8-tetrahydrofolate + L-homocysteine = (6S)-5,6,7,8-tetrahydrofolate + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:11172"/>
            <dbReference type="ChEBI" id="CHEBI:18608"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="2.1.1.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 20 23">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3 20 22">
            <name>methylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:28115"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 20">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetH route): step 1/1.</text>
    </comment>
    <comment type="domain">
        <text evidence="20">Modular enzyme with four functionally distinct domains. The isolated Hcy-binding domain catalyzes methyl transfer from free methylcobalamin to homocysteine. The Hcy-binding domain in association with the pterin-binding domain catalyzes the methylation of cob(I)alamin by methyltetrahydrofolate and the methylation of homocysteine. The B12-binding domain binds the cofactor. The AdoMet activation domain binds S-adenosyl-L-methionine. Under aerobic conditions cob(I)alamin can be converted to inactive cob(II)alamin. Reductive methylation by S-adenosyl-L-methionine and flavodoxin regenerates methylcobalamin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the vitamin-B12 dependent methionine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.13" evidence="6 20"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313616.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0283"/>
    <dbReference type="EnsemblBacteria" id="ABA03550">
        <property type="protein sequence ID" value="ABA03550"/>
        <property type="gene ID" value="Nwi_0283"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0283"/>
    <dbReference type="eggNOG" id="COG0646">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1410">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004914_2_0_5"/>
    <dbReference type="OMA" id="ADCIAMS"/>
    <dbReference type="OrthoDB" id="118138at2"/>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00081"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008705">
        <property type="term" value="F:methionine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042558">
        <property type="term" value="P:pteridine-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02069">
        <property type="entry name" value="methionine_synthase_B12_BD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1240.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.196.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003759">
        <property type="entry name" value="Cbl-bd_cap"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036724">
        <property type="entry name" value="Cobalamin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003726">
        <property type="entry name" value="HCY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036589">
        <property type="entry name" value="HCY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033706">
        <property type="entry name" value="Met_synthase_B12-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011822">
        <property type="entry name" value="MetH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036594">
        <property type="entry name" value="Meth_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000489">
        <property type="entry name" value="Pterin-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004223">
        <property type="entry name" value="VitB12-dep_Met_synth_activ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037010">
        <property type="entry name" value="VitB12-dep_Met_synth_activ_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02607">
        <property type="entry name" value="B12-binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02965">
        <property type="entry name" value="Met_synt_B12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00809">
        <property type="entry name" value="Pterin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02574">
        <property type="entry name" value="S-methyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000381">
        <property type="entry name" value="MetH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01018">
        <property type="entry name" value="B12-binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47644">
        <property type="entry name" value="SSF47644"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="SSF51717"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52242">
        <property type="entry name" value="SSF52242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56507">
        <property type="entry name" value="SSF56507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82282">
        <property type="entry name" value="SSF82282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02082">
        <property type="entry name" value="metH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50974">
        <property type="entry name" value="ADOMET_ACTIVATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51337">
        <property type="entry name" value="B12_BINDING_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50970">
        <property type="entry name" value="HCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50972">
        <property type="entry name" value="PTERIN_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 20" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 20 22" id="KW-0846">Cobalamin</keyword>
    <keyword evidence="17 20 21" id="KW-0170">Cobalt</keyword>
    <keyword evidence="13 20 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16 20" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="8 20 24" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0677">Repeat</keyword>
    <keyword evidence="12 20 22" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="11 20 24" id="KW-0808">Transferase</keyword>
    <keyword evidence="15 20 21" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Hcy-binding" evidence="25">
        <location>
            <begin position="12"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Pterin-binding" evidence="26">
        <location>
            <begin position="362"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="domain" description="B12-binding N-terminal" evidence="29">
        <location>
            <begin position="654"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="domain" description="B12-binding" evidence="28">
        <location>
            <begin position="760"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="domain" description="AdoMet activation" evidence="27">
        <location>
            <begin position="912"/>
            <end position="1244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Methylcob(III)alamin binding" evidence="22">
        <location>
            <begin position="770"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="22">
        <location>
            <begin position="1206"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21 23">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21 23">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21 23">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="698"/>
        </location>
        <ligand>
            <name>methylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:28115"/>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="21">
        <location>
            <position position="773"/>
        </location>
        <ligand>
            <name>methylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:28115"/>
        </ligand>
        <ligandPart>
            <name>Co</name>
            <dbReference type="ChEBI" id="CHEBI:27638"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="818"/>
        </location>
        <ligand>
            <name>methylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:28115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="822"/>
        </location>
        <ligand>
            <name>methylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:28115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="875"/>
        </location>
        <ligand>
            <name>methylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:28115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="962"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="1151"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00025552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00031040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRNR" id="PIRNR000381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR000381-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR000381-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS51337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="EMBL" id="ABA03550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1290" mass="140731" checksum="E37A08A59E688DAE" modified="2005-10-11" version="1">MTRVTKSSNTIADKFRALARERILVLDGAMGTMIQNRGFDEAAFRGERFKNFHRDVRGNNDLLILTQPQAIEDIHAQYLRAGADLVATNTFSSTSIAQADYDMSDLVYELNREGAKLARSAAEKVSAEDGKPRFVVGAIGPTNRTASISPDVANPGYRAVTFDDLRKSYGEQINGLLDGGADLLLVETIFDTLNAKAALYAIAEIGEQRGIDIPVMVSGTITDKSGRLLSGQLPEAFWHSVRHAGPITIGFNCALGAEDLRAHVAEIGRVADTLVCAYPNAGLPNEFGEYDESPDYMARLIGEFAQAGLVNVVGGCCGTTPDHIAAIAAAVAPHKPRVVPVIEPRLRLSGLEPFSLTSDIPFVNVGERTNVTGSAKFRNLIKAGDYAAALDVARNQVENGAQIIDVNMDEGLLDSEAAMVTFLNLIAAEPDIARVPVMVDSSKFSVIEAGLKCLQGKPVVNSISLKEGEEKFLHEARIARRHGAAVVVMAFDEQGQADTCQRKMEICKRAYDILVEQVGFPPEDIIFDPNIFAIATGLEEHNNYGVDFIEATRWIRQNLPHAHISGGVSNLSFSFRGNEPVREAMHSVFLYHAIKAGMDLGIVNAGQMVIYDDIDPELRQVCEDVVLNRDPDAGERLLNLAEKFRGQGKQAKEADLSWREWPVEKRLSHALVNGITEYIEQDTEEARKSADRPLSVIEGPLMAGMNVVGDLFGDGKMFLPQVVKSARVMKHAVAYLMPFMEEEKARNQAAGIEGAGSTSAGKILLATVKGDVHDIGKNIVGIVLQCNNYEVIDLGVMVPAAKIIETAKAEKADIVGLSGLITPSLDEMGYFAAELERQGLNLPLLIGGATTSRVHTAVKIDPNYRNGPVIHVNDASRAVGVVASLMSPERREAYAAGVRAEYAKISAAHFRAQQDKKRLPLAAARANAVPIDWSSYRPVKPTFLGTKAFADYPLEELVEVIDWTPFFQVWELAGRFPAILDDKVVGETARALYDDARKMLDRIVKEKWFTASSVIGFWPANRIGDDIAVYSDESRTTQIATYHTLRQQLEKREGRHNAALADFIAPKETGIADYIGGFAVTAGLGEDEIAARFKNANDDYSSILVKALADRLAEAFAERMHQRVRTEFWGYAPDEALTNDQLIREEYVGIRPAPGYPAQPDHTEKATLFRLLDATRATGVELTESFAMWPGSSVSGLYYSHPESQYFGVGKIERDQVEDYAARKGMEVAEIERWLASILNYIPGQDASGKPATTPVPAPANDASADVANHPPGCQCAVHLRLRKTTARAG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVZ7</accession>
    <name>NTPPA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">dTTP/UTP pyrophosphatase</fullName>
            <shortName evidence="1">dTTPase/UTPase</shortName>
            <ecNumber evidence="1">3.6.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Nucleoside triphosphate pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Nucleotide pyrophosphatase</fullName>
            <shortName evidence="1">Nucleotide PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">Nwi_0277</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Nucleoside triphosphate pyrophosphatase that hydrolyzes dTTP and UTP. May have a dual role in cell division arrest and in preventing the incorporation of modified nucleotides into cellular nucleic acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dTTP + H2O = diphosphate + dTMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28534"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37568"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="EC" id="3.6.1.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + UTP = diphosphate + H(+) + UMP</text>
            <dbReference type="Rhea" id="RHEA:29395"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="EC" id="3.6.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Maf family. YhdE subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.9" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313610.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVZ7"/>
    <dbReference type="SMR" id="Q3SVZ7"/>
    <dbReference type="STRING" id="323098.Nwi_0277"/>
    <dbReference type="EnsemblBacteria" id="ABA03544">
        <property type="protein sequence ID" value="ABA03544"/>
        <property type="gene ID" value="Nwi_0277"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0277"/>
    <dbReference type="eggNOG" id="COG0424">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040416_2_0_5"/>
    <dbReference type="OMA" id="RIDGDFY"/>
    <dbReference type="OrthoDB" id="1469203at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036218">
        <property type="term" value="F:dTTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035529">
        <property type="term" value="F:NADH pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036221">
        <property type="term" value="F:UTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00555">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00528">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003697">
        <property type="entry name" value="Maf-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43213">
        <property type="entry name" value="PTHR43213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02545">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006305">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="SSF52972"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00172">
        <property type="entry name" value="maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000267354" description="dTTP/UTP pyrophosphatase">
        <location>
            <begin position="1"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="1">
        <location>
            <position position="15"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="1">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="1">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00528"/>
        </source>
    </evidence>
    <sequence length="207" mass="22404" checksum="BF8EA697ECFDC665" modified="2005-10-11" version="1">MSGRPKLVLASGSPRRLSLLNQAGIEPDALRPVDIDETPTKGELPRACANRLARAKADAALRALQIDDELRGSFILAADTVVAVGRRILPKADLVDEASQCLRLLSGRNHRVYTAVCLVTPKENFRQRLVETRVRFKRLSEEDIQAYIGSGEWRGKAGGYAIQGIAGSFVVKLVGSYTNVVGLPLNESIALLGGEEFPIRAGWLDAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW07</accession>
    <name>Q3SW07_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA_13 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0267</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313600.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0267"/>
    <dbReference type="EnsemblBacteria" id="ABA03534">
        <property type="protein sequence ID" value="ABA03534"/>
        <property type="gene ID" value="Nwi_0267"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0267"/>
    <dbReference type="eggNOG" id="COG0497">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016086_0_0_5"/>
    <dbReference type="OMA" id="QVIIFTH"/>
    <dbReference type="OrthoDB" id="538212at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026866">
        <property type="entry name" value="CR006_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13166">
        <property type="entry name" value="AAA_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_13" evidence="1">
        <location>
            <begin position="504"/>
            <end position="711"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="872" mass="96067" checksum="60396DD4F32ABF95" modified="2005-10-11" version="1">MSAAPSQSAPLTEKAALADLVTWAEKRPAWQKDALRRLVTGETLDDQAIAELTELCLDPKRAHAPISQSHIGAVTSSAEPISLVSIKNPTAINALASQQELSFEQSGLTVIYGDNASGKSGYVRVLKHACRSRDAKFAIHRDLEDKTDTPQSAEIFFARGSTTEQFSWTPDGEPHSDLPSVSIFDSRSASIHVESTNDVAYIPRPMRLLETLADACDRIKAALDSKIATLNKQVPLAIAAPSLSRETAAGSYVFGLNAKSNIPQLDKLAVLSEAENARLTTLETDFAQDPKRAVARLDNQRGEIERIQTTLQTLIGATNAAAFSEIGTLRKAHSEAVEAASIASQKLFSASPLPEVGQAAWQKLWEAARTYADTVAYPGKTFPSSDPDTLCVLCQQPLGADAIARQETFESFVKSSTKSDEAAARAKLDTALVQLEARTLRLDGVLKHSKFLATEIGDASLARQVRRAFIVVLWRLRAMLRAYGAPKPLGTDPLPALSNLAADLKTRAAQLSADTQSDDYKKLKSEYLELKDRAALAPLLPDIKAQIARLQESETINTALKATAKKPITDKNKELSDRMVTDALRGRFAREIDKLKLSRMPVELRKEKDRQAVSYFRVALVERPDAKVGEIFSEGEHRCVALAAFLAELVTAQRYSGIVFDDPMSSLDHIHRGAVASRLVEEAEHRQVVVFTHDLVFLYELRREAEKANRPIAFRNVRRQQNRPGYVENELPDKAKSGLEMCNALRSSIKAAKSGFDHLNDTQRTIFCKGAIGQMRDAWEQAICDYIRPVLERFDNKVKPTSMFKLGILTEDDVKCVMAAQARLSEDLHSSAQALNPEAISLNDLLSEVKALEDWIQSIRERQNNATRPTIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW59</accession>
    <name>Q3SW59_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0214</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0214"/>
    <dbReference type="EnsemblBacteria" id="ABA03482">
        <property type="protein sequence ID" value="ABA03482"/>
        <property type="gene ID" value="Nwi_0214"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0214"/>
    <dbReference type="eggNOG" id="COG3293">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055261_2_6_5"/>
    <dbReference type="OMA" id="TWERVFT"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025161">
        <property type="entry name" value="Put_IS4/IS5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13340">
        <property type="entry name" value="DUF4096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="106" mass="12179" checksum="6CAF82B1A552BA41" modified="2005-10-11" version="1">MLPGKASDPGRTGSDNRLFVNGCLWVLRSGAHWCDLPERYGRWKTVHRRFSRWCHAGVWERVFSTLTADRDNQYLMLDSTIVRAHQQAATGKGGPRIRRWGVPEVD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW65</accession>
    <name>KCY_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cytidylate kinase</fullName>
            <shortName evidence="1">CK</shortName>
            <ecNumber evidence="1">2.7.4.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cytidine monophosphate kinase</fullName>
            <shortName evidence="1">CMP kinase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cmk</name>
        <name type="ordered locus">Nwi_0208</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + CMP = ADP + CDP</text>
            <dbReference type="Rhea" id="RHEA:11600"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + dCMP = ADP + dCDP</text>
            <dbReference type="Rhea" id="RHEA:25094"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57566"/>
            <dbReference type="ChEBI" id="CHEBI:58593"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytidylate kinase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.25" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313544.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SW65"/>
    <dbReference type="SMR" id="Q3SW65"/>
    <dbReference type="STRING" id="323098.Nwi_0208"/>
    <dbReference type="PRIDE" id="Q3SW65"/>
    <dbReference type="EnsemblBacteria" id="ABA03476">
        <property type="protein sequence ID" value="ABA03476"/>
        <property type="gene ID" value="Nwi_0208"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0208"/>
    <dbReference type="eggNOG" id="COG0283">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079959_0_1_5"/>
    <dbReference type="OMA" id="RAITWWM"/>
    <dbReference type="OrthoDB" id="776861at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036430">
        <property type="term" value="F:CMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036431">
        <property type="term" value="F:dCMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006220">
        <property type="term" value="P:pyrimidine nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02020">
        <property type="entry name" value="CMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00238">
        <property type="entry name" value="Cytidyl_kinase_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003136">
        <property type="entry name" value="Cytidylate_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011994">
        <property type="entry name" value="Cytidylate_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02224">
        <property type="entry name" value="Cytidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00017">
        <property type="entry name" value="cmk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000048245" description="Cytidylate kinase">
        <location>
            <begin position="1"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00238"/>
        </source>
    </evidence>
    <sequence length="212" mass="22587" checksum="E7ACB18A2D273BC8" modified="2005-10-11" version="1">MIIAIDGPAASGKGTLGKRLAAHYHFRHLDTGVIYRAVAKALLDAGADPADEARAIAAALELDPERFGHPALKARAIGEAASVVSTFPKVREALLTFQRQFAAEPPGAVLDGRDIGTVICPNADVKIFVEADPVVRARRRTLEARARGEEVDEAVVLADILKRDERDRNRPVAPLKAAADAHVLDNSNLDIEAGLKAAIAIVESVRVRSGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW83</accession>
    <name>Q3SW83_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Amino acid/amide ABC transporter substrate-binding protein, HAAT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0190</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0190"/>
    <dbReference type="EnsemblBacteria" id="ABA03458">
        <property type="protein sequence ID" value="ABA03458"/>
        <property type="gene ID" value="Nwi_0190"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0190"/>
    <dbReference type="eggNOG" id="COG0683">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049010_0_0_5"/>
    <dbReference type="OMA" id="TGLWDNP"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028081">
        <property type="entry name" value="Leu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13458">
        <property type="entry name" value="Peripla_BP_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peripla_BP_6" evidence="2">
        <location>
            <begin position="71"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="407" mass="41576" checksum="9461C3C666614C8B" modified="2005-10-11" version="1">MMMKDPFAPKPERHGPTRRTALGLVVGAPLLGACSGVQQTLTNGFSSPPGSSPQPESTAGPQQGATIGTGQVKVGLILPLSAAGNASIAAQSMRNAAEMALSEFQNPNVQVLVKDAAGTARGAQQGAQQALDEGAEIILGPLFAASVPGVAQVARTRGVSVIAFSTDSSIAGRGVYLLSFLPESDVTRIVEYAASTGKRSFAAMVPENAYGNVVEAAFKQTVGRRGGRIAAFEKYGSDRASAARNVAQSLRSADALLIADDGDTVVSVAAALTAAGANLRNVQLLGTGLWDNPRVFSSTTLHGGLYAAPDPAGFRAFAGRYRTKFGGEPVRTATLAYDAVALVAALARTQGSQRFAAETLTNASGFAGIDGLFRFRSDGTNQRGLAVMRVASGGGQSVAGSPKSFGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWA7</accession>
    <name>Q3SWA7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NAD-dependent epimerase/dehydratase</fullName>
            <ecNumber evidence="3">1.1.1.145</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0166</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.145" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0166"/>
    <dbReference type="EnsemblBacteria" id="ABA03434">
        <property type="protein sequence ID" value="ABA03434"/>
        <property type="gene ID" value="Nwi_0166"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0166"/>
    <dbReference type="eggNOG" id="COG0702">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_6_5_5"/>
    <dbReference type="OMA" id="FFNRFAA"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003854">
        <property type="term" value="F:3-beta-hydroxy-delta5-steroid dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102294">
        <property type="term" value="F:cholesterol dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="2">
        <location>
            <begin position="40"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="353" mass="37201" checksum="B3FD9CED1E9540B4" modified="2005-10-11" version="1">MAGWTARRLTCRGTAEYPFSRPDTNAFEDQIMASNSDTLVTVFGGSGFLGRYVVRALAKRDYRIRVGVRRPELAGHLQPLGKVGQIHAVQVNLRHPASVRAAMRGSEVAVNLVGILTKSGRQTFDAVVAKGAATVAETAAAAGARLVHVSAIGADPASASAYARAKASGEKAVLAAVPSATILRPSVMFGPEDQFANRFAALALISPMLPLIGGGATRMQPVHVGDVATAVADAVDGRTKAGAVYELGGPDVLSMREIMRIILRVIERERMLVPVPFLMARLMAMGLQFAPGPLKLTPDQVALLRTDNVVSNTATAAGLTLQGLGIAPDSMEAVVPQYLWRFRKAGQFAPKGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWB0</accession>
    <name>Q3SWB0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 8">2-dehydropantoate 2-reductase</fullName>
            <ecNumber evidence="4 8">1.1.1.169</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 8">Ketopantoate reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_0163</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>(R)-pantoate + NADP(+) = 2-dehydropantoate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:16233"/>
            <dbReference type="ChEBI" id="CHEBI:11561"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.169"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the ketopantoate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.169" evidence="4 8"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313500.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0163"/>
    <dbReference type="EnsemblBacteria" id="ABA03431">
        <property type="protein sequence ID" value="ABA03431"/>
        <property type="gene ID" value="Nwi_0163"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0163"/>
    <dbReference type="eggNOG" id="COG1893">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031468_6_1_5"/>
    <dbReference type="OMA" id="WEKWVFL"/>
    <dbReference type="OrthoDB" id="427052at2"/>
    <dbReference type="UniPathway" id="UPA00028">
        <property type="reaction ID" value="UER00004"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008677">
        <property type="term" value="F:2-dehydropantoate 2-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003710">
        <property type="entry name" value="ApbA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013752">
        <property type="entry name" value="KPA_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013332">
        <property type="entry name" value="KPR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02558">
        <property type="entry name" value="ApbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08546">
        <property type="entry name" value="ApbA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00745">
        <property type="entry name" value="apbA_panE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0521">NADP</keyword>
    <keyword evidence="8 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 8" id="KW-0566">Pantothenate biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ApbA" evidence="9">
        <location>
            <begin position="3"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="ApbA_C" evidence="10">
        <location>
            <begin position="179"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA03431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="306" mass="32344" checksum="E6D0806BAFC1DB3D" modified="2005-10-11" version="1">MRILVVGAGATGGYFGGCLLRAGQDVTFLVRPKRATELASAGLVIRSPHGDFTLDNPPTVQADGVSETFDLVLLSCKAFDLEDAIASFARAVGPTTAIVPLLNGMLHLETLDAAFSRERVLGGLCSLAVTLNEKREVVHLAPMHSLRFGERDGGASERVRAIAEAFAGARCGAQPSETIIQDMWEKWVFLAALAGATCVMRASIGQIVAAPGGRDFMLALTDEAAGIAKAAGYAPREEFLSGATAMLTAAGSQLTASMFRDIETGARVEADHIMGDLIARAEAAKLPVPKLRTIHTHLKAYENRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWD9</accession>
    <name>Q3SWD9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Hsp33 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0134</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313471.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0134"/>
    <dbReference type="EnsemblBacteria" id="ABA03402">
        <property type="protein sequence ID" value="ABA03402"/>
        <property type="gene ID" value="Nwi_0134"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0134"/>
    <dbReference type="eggNOG" id="COG1281">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054493_0_1_5"/>
    <dbReference type="OMA" id="DMQCECC"/>
    <dbReference type="OrthoDB" id="1406579at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00498">
        <property type="entry name" value="Hsp33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.30.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000397">
        <property type="entry name" value="Heat_shock_Hsp33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016154">
        <property type="entry name" value="Heat_shock_Hsp33_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016153">
        <property type="entry name" value="Heat_shock_Hsp33_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023212">
        <property type="entry name" value="Hsp33_helix_hairpin_bin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30111">
        <property type="entry name" value="PTHR30111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01430">
        <property type="entry name" value="HSP33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005261">
        <property type="entry name" value="Heat_shock_Hsp33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118352">
        <property type="entry name" value="SSF118352"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64397">
        <property type="entry name" value="SSF64397"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="334" mass="36318" checksum="54EFACDC89519A46" modified="2005-10-11" version="1">MTSQTSDPAALREPSVRAPSSVPVDDAVIPFEVGALDLRGRLTRLGPALDELLARHDYPAPVAKLLGEAIVLTTLLGSSLKFEGRFILQTQTDGPVSFLIVDFRAPARLRAYARFDEARLGDASDAGALLGRGHLAMTIDQGPDMSRYQGLVALEGGSLEDAAHEYFLRSEQIPTRVRLAVGEEWRRDGSGPKRGWRGGGMLLQFLPKAPERARQADLPPGDAPDGEAVHTVADDDAWVEGQSLIGTVEDLELIDPDLSGERLLYRLFHERGVMVFSSQALHARCSCSRDAVEAMLKSFALEDRSGMVQDGKVAVTCEFCNSVYEFTPREAGVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN00</accession>
    <name>Q3SN00_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_3094</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3094"/>
    <dbReference type="EnsemblBacteria" id="ABA06341">
        <property type="protein sequence ID" value="ABA06341"/>
        <property type="gene ID" value="Nwi_3094"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3094"/>
    <dbReference type="eggNOG" id="ENOG5032NJM">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_177741_0_0_5"/>
    <dbReference type="OMA" id="WRSERMS"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="104" mass="11645" checksum="CAD859894D1387DC" modified="2005-10-11" version="1">MFGLISQRPSVATGDRVPAMRFVFPHSHAFRRGRVTVDDDQKSGPGCLVEFGDGVTVIAEWYAVGNAIHLSIPAYHTAKGTQVSRWTWRLVQSKDGLWRSERMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN07</accession>
    <name>Q3SN07_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_3087</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3087"/>
    <dbReference type="EnsemblBacteria" id="ABA06334">
        <property type="protein sequence ID" value="ABA06334"/>
        <property type="gene ID" value="Nwi_3087"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3087"/>
    <dbReference type="eggNOG" id="COG2963">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027402_36_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002514">
        <property type="entry name" value="Transposase_8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01527">
        <property type="entry name" value="HTH_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="46"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="89" mass="9894" checksum="53608BF59B52D2AB" modified="2005-10-11" version="1">MLEGVRGEESIAELCRREGIASSMYYGWSKEFLDAGKRRLAGDTARAATSDEVKELRREAQALKEAVADLTLENRLLKKSMLADGEDDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN82</accession>
    <name>Q3SN82_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, PadR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_3009</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316179.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06259">
        <property type="protein sequence ID" value="ABA06259"/>
        <property type="gene ID" value="Nwi_3009"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3009"/>
    <dbReference type="eggNOG" id="COG1695">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063440_1_0_5"/>
    <dbReference type="OMA" id="ISEAPRH"/>
    <dbReference type="OrthoDB" id="1706427at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PadR" evidence="1">
        <location>
            <begin position="19"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="141" mass="15617" checksum="792EC169EA89FA01" modified="2005-10-11" version="1">MTAPSSKGVAEGDLRLVALSLISEAPRHGYDIIQTVEKMTADWFLPSPNVVHPMLAKLVEERCLTASADPPRLFSITDEGRAYLEQHRDRADAVLNRLKALGERVTRWRQALGVAERQKEADADSTEGLLDVLARAAMPRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN95</accession>
    <name>Q3SN95_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Methyl-accepting chemotaxis sensory transducer</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_2996</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316168.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2996"/>
    <dbReference type="EnsemblBacteria" id="ABA06246">
        <property type="protein sequence ID" value="ABA06246"/>
        <property type="gene ID" value="Nwi_2996"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2996"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2770">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_27_5"/>
    <dbReference type="OMA" id="MERIEHS"/>
    <dbReference type="OrthoDB" id="429006at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0807">Transducer</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="324"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="7">
        <location>
            <begin position="347"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="5">
        <location>
            <begin position="442"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="6">
        <location>
            <begin position="594"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA06246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="698" mass="74137" checksum="3E02CA5514FBCFBA" modified="2005-10-11" version="1">MKAKISVILPAIVSALVMIGALSAGIMAYQAYEQRQQSEAFLEVNHISQLLLRSAGQWAVERGLTNAPLMSPDALPAERRPQILEARQTADRAFSEAARLLREHTAMKSAESRISEAESAFQAFVGFRARLDENLARRGPERSAEVVESFAPTITRLIEVSGNTLRLTLETLTRPPTAALTQLVSLRHLTAQMAENAGRERALFGGIVSSRARLSTSSLRTIARSRGYVELAWETISPIRMRADLPPKVADAISGVEKDYFQRYGQTRDAVFAASETGEYKISGNDYVAQATTAINSILRLADAVGEAADQEAARVAAGSRSQLLSSALILLGCLALALISFWIAFSRILRPMAALTTAMGELAKGNFAVVLPGLERKDEMGDMARAVETFKIKAEQKARDEAEAKMKQDQLAAQQRKADMHRLADGFEAAVGAVIQTVSSAATQLEDSATALTQTAEQTHQLSSAVAAASEEATVNVQSVASASEEMAASVNEISRQVQEASRIASDAAQKAHRADQRINSLSQASTKIGDVVELINTIASQTNLLALNATIEAARAGEAGRGFAVVASEVKALAEQTAKATAEIGQQITSIQSATNDSVDNMKEIGIVIGRIAEISATIASAVEEQASATQEISRNVQQAATGTSEVSSNIIEVSNGASVTGSASTQALAAAKSLAGDTDRLKQEVEKFLATIRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNC5</accession>
    <name>Q3SNC5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2966</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2966"/>
    <dbReference type="EnsemblBacteria" id="ABA06216">
        <property type="protein sequence ID" value="ABA06216"/>
        <property type="gene ID" value="Nwi_2966"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2966"/>
    <dbReference type="HOGENOM" id="CLU_2509319_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="85" mass="9662" checksum="1AF189C7C9622E7D" modified="2005-10-11" version="1">MEGVFAVDPACVNRRHPLSDETLGGMVRKIASFLLRACKANLPNPHHYDNHEYGNHGLPASCGFEVRDRRRGMDALHFCKEFPRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNC8</accession>
    <name>Q3SNC8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2963</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316135.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06213">
        <property type="protein sequence ID" value="ABA06213"/>
        <property type="gene ID" value="Nwi_2963"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2963"/>
    <dbReference type="HOGENOM" id="CLU_198738_0_0_5"/>
    <dbReference type="OrthoDB" id="2007990at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="71" mass="7762" checksum="670A5D91B35810C2" modified="2005-10-11" version="1">MSQVPSQKFGAEYVAHLRELLDIAAEEIGPEHRASATKAKITRTRIRNKDGLPEARHFTSAASTAGEDRTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SND6</accession>
    <name>Q3SND6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2955</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06205">
        <property type="protein sequence ID" value="ABA06205"/>
        <property type="gene ID" value="Nwi_2955"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2955"/>
    <dbReference type="HOGENOM" id="CLU_2082306_0_0_5"/>
    <dbReference type="OMA" id="DATSCYR"/>
    <dbReference type="OrthoDB" id="39959at41294"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229054" evidence="1">
        <location>
            <begin position="22"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="117" mass="12656" checksum="B7B3F4627A18D11B" modified="2005-10-11" version="1">MAASRWMVFLIVVVPIAPAQAMSRHVAVSAGSFFAAAARCEEQNLIAPGQTGHLLEALDPYLSKSDKANMAAGDARGKKISKVFVESEKKWVSFTPDATSCYRVQGVLDDYRAQLGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNE0</accession>
    <name>Q3SNE0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lysophospholipase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2951</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2951"/>
    <dbReference type="ESTHER" id="nitwn-q3sne0">
        <property type="family name" value="Monoglyceridelipase_lysophospholip"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06201">
        <property type="protein sequence ID" value="ABA06201"/>
        <property type="gene ID" value="Nwi_2951"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2951"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026209_10_1_5"/>
    <dbReference type="OMA" id="AFDWRGQ"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydrolase_4" evidence="1">
        <location>
            <begin position="69"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="344" mass="37853" checksum="676B103B59CF3DF5" modified="2005-10-11" version="1">MEAVQARSRGGTRLLSGDPLFQVSPPAGSVMKLLSIPANPVPENCVSGAVRTSDGVDLRFARWPSPQGRKGTVCLFTGRGEQIEKYFETVRDLRDRGFAVAMIDWRGQGHSLRQLPDPRKGHVRRFSDYENDVRAFVRQVVLPDCPPPYFALAHSMGGAVLLRVARAGNRWFDRMVLSAPMIDLPGRRTSLPVRTLLRAMRLLGQGGRYVPGGSAQITGMGGFAGNFLTSDPVRFARNVAILQEDPTLGIASPTVAWADTAFQTMHDFRDASYPARIRQPLLLIAAGSDAIVSTPAIEQFAYHLRSGLHLVIAGSKHEILQEQDRYRAQFWAAFDAFVPGTPLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNH9</accession>
    <name>Q3SNH9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Secretion protein HlyD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2912</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316092.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2912"/>
    <dbReference type="EnsemblBacteria" id="ABA06162">
        <property type="protein sequence ID" value="ABA06162"/>
        <property type="gene ID" value="Nwi_2912"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2912"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018816_13_3_5"/>
    <dbReference type="OMA" id="GAFHLNN"/>
    <dbReference type="OrthoDB" id="1174564at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032317">
        <property type="entry name" value="HlyD_D23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006143">
        <property type="entry name" value="RND_pump_MFP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16576">
        <property type="entry name" value="HlyD_D23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01730">
        <property type="entry name" value="RND_mfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HlyD_D23" evidence="3">
        <location>
            <begin position="79"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="169"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA06162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="418" mass="45248" checksum="2A9A666F47BCF128" modified="2005-10-11" version="1">MRGLASLRRVREIPRIAIGLASALVLVGTAAVVLNIDRGSAARNPTLDSEIKQPDGKLRLTSSQWGTVTVQPVEQHAFRSEFRTEGKIAIDQNLSTRIFAPYAGRVTQLMVASGDQVQKGQLLFVIAAADSLDAQRDFVVALTTRNKAVSQVNLAQIVERRMSSLAKDKAASQREWQEAQTNLTAAENDSRSAEIALQAARNRLRLVGKTDAEIDAFEKTGALTPDAPVYAPLAGTVLHRKVGIGQYVSAGAGDSDPALLIGDTSKVWIVAYVRETDVAKVKIGERLTFKVLAQPDRVFETRIDYVAPGIDPDNRRLLVRASVDNADGLLKPEMFASVTIVASEGAPMPAIPLESVIYEGSNAHVWIVGDDQSVELRQITLGQSDGRLIQVLDGLHPGEKIVTRGSLFIDRMTVAKQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNL6</accession>
    <name>Q3SNL6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2875</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316057.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2875"/>
    <dbReference type="EnsemblBacteria" id="ABA06125">
        <property type="protein sequence ID" value="ABA06125"/>
        <property type="gene ID" value="Nwi_2875"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2875"/>
    <dbReference type="HOGENOM" id="CLU_119014_0_0_5"/>
    <dbReference type="OMA" id="SMTEGAN"/>
    <dbReference type="OrthoDB" id="2211794at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="182" mass="19804" checksum="A14D72B73452568F" modified="2005-10-11" version="1">MAEQNGYLAELAKLREADSQAYLKQWIVWLAVGSAAGAVALLSLAAQSGNPNYAIRFLLPSLWAFAAGVIFAGASIALLSKKLGAMAEHFVHAYNRAQMEIKIAETPEVFASPQHLADGANAARNKLISEQHKAHAFAEKAWVSQTLWSRMWTGCVIVSATRFIFGIVWPLIQLSLGRTLIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNN2</accession>
    <name>Q3SNN2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ParB-like nuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2859</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2859"/>
    <dbReference type="EnsemblBacteria" id="ABA06109">
        <property type="protein sequence ID" value="ABA06109"/>
        <property type="gene ID" value="Nwi_2859"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2859"/>
    <dbReference type="eggNOG" id="COG1475">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019174_1_0_5"/>
    <dbReference type="OMA" id="RNVRTTP"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB/Sulfiredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02195">
        <property type="entry name" value="ParBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00470">
        <property type="entry name" value="ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="SSF110849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ParB" evidence="2">
        <location>
            <begin position="61"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="699"/>
            <end position="726"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="726" mass="79720" checksum="7FAB70C9311ADECE" modified="2005-10-11" version="1">MNRERIGTMARTSKKTKAVPIATVVEAAADVDVRSVGEAEAVAATESGAETVSVAASGETVFIPLNKLKKSPRNARKIPHGEADIEAYAASIAAKGILQNLVVEPERDEEGQPTGFYFVTIGEGRRLAQLLRAKRKQIKKTEPIRCVIDTANDPFEISLDENVTRTAMHPADQFEAFRELAETRGWGAEEIAARFGVTAHVVKQRLRLGAVSPKLMQVYRDGGLTLDQLMAFAITEDHSRQEQVYESLSYNRDPSFIRRELMQANVPASDRRAIFIGPESYSEAGGNIVRDLFPEDRGGFYEDVALLDRLVMEKLESIAFRVRGDEGWKWWEAHIDFPHAHGMRRVYPHPVELSPQDEADYAAAQEEYDRLSSEYEDADELPDDVDQRFGELEAEIEHIDAKRHAYDVDDICRSGVFVVLDHDGGARVERGFVRPEDEKPEAEEASDGETVIDRVRVNDDGEVIEDGEDGNHAADLEAEDEYAEEDGKPLSDLLIRDLTAHRTLGLRLALGGQPDMALIAVTHALAAQTFYHAVDAHCLDIRPNSAHLASHADGIEDTAAAKALADRHAGWAADMPRDVADLWGFIAGLDHASVMALFAHCASQTINAVKLPWERKPRAHATADKLATALALDMTANWTPTVRTYLGRVTKALILDAVREAVSDEAADRLASVKKQPMAEAAEQLLAGTGWLPPLMRTTPVAEAESGPVEDIAAPEGNEGFPEAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNP6</accession>
    <name>Q3SNP6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Outer membrane autotransporter barrel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2845</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316035.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2845"/>
    <dbReference type="EnsemblBacteria" id="ABA06095">
        <property type="protein sequence ID" value="ABA06095"/>
        <property type="gene ID" value="Nwi_2845"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2845"/>
    <dbReference type="eggNOG" id="COG3468">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_452487_0_0_5"/>
    <dbReference type="OMA" id="NNWFIEP"/>
    <dbReference type="OrthoDB" id="2018542at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019867">
        <property type="term" value="C:outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005546">
        <property type="entry name" value="Autotransporte_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036709">
        <property type="entry name" value="Autotransporte_beta_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006315">
        <property type="entry name" value="OM_autotransptr_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027385">
        <property type="entry name" value="OMP_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03797">
        <property type="entry name" value="Autotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13505">
        <property type="entry name" value="OMP_b-brl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00869">
        <property type="entry name" value="Autotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103515">
        <property type="entry name" value="SSF103515"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01414">
        <property type="entry name" value="autotrans_barl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51208">
        <property type="entry name" value="AUTOTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229062" evidence="1">
        <location>
            <begin position="38"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="domain" description="Autotransporter" evidence="2">
        <location>
            <begin position="92"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="652" mass="68105" checksum="725B32866B5AD2C9" modified="2005-10-11" version="1">MNVVVRASMPGNGALRRRVVAGGAFAFALSASSGAIAACTGSGTLVGLGADVLPFTAGGAAVNSLVSAINATNTVFLTQSTAFVSAPANAAPNQEGGGIWVRGIGGEITTRNNTTTSNVSVGGVASPGQIDCNNSSRLSFSGVQLGTDIAQLNWNGWNVHLGTTVGYLGARSRDTSSAGSENPFGGTFQNSLQVPFVGMYAAATHGGFFVDGQIRLDYYQNTLSDPNVSGIFNQKLDARGLSFAGNFGYHHQLPNNWFIEPSAGIVVSRVQVDPLNITGTLVLPTAGGISLPGTLRVDDMYSALGRWSLRAGTNIVSGNIAWQPFGTASVFHEFQGGANASFTGYFPIPGFDVAGNVRTSSIGTYGQFGLGVAAQVLNTGWLGYLRADYRTGSNIEGYSLNGGLRYQFSPDQVAGAPLFAKAPAMGVAPYNWTGFYIGASTGVLNGRTDWSFPGFGTGANPRFAGFAGGGQIGYDWQTGPWVVGIEGAFNASNANGARSCPNALFYTCRNEIDWLGTATAKLGYALWSRSLVYLRGGAAFGNTRIVSTCNTGPNGQYIDYSIPALITQPGCGASSSRTAVGWTIGYGAEFALSRNWTVRAESNYFDLGTERHSLAQGGGIPPIQVDVRNHGFISTVGLNYRFPIEPVLAARY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNS8</accession>
    <name>Q3SNS8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ferredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_2810</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 7">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316008.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2810"/>
    <dbReference type="EnsemblBacteria" id="ABA06063">
        <property type="protein sequence ID" value="ABA06063"/>
        <property type="gene ID" value="Nwi_2810"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2810"/>
    <dbReference type="eggNOG" id="COG1146">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_0_0_5"/>
    <dbReference type="OMA" id="FVTDKCI"/>
    <dbReference type="OrthoDB" id="1873445at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000813">
        <property type="entry name" value="7Fe_ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022569">
        <property type="entry name" value="Fd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11953">
        <property type="entry name" value="DUF3470"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00354">
        <property type="entry name" value="7FE8SFRDOXIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4 7" id="KW-0408">Iron</keyword>
    <keyword evidence="5 7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="31"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA06063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="112" mass="12407" checksum="179518C7397A3ABC" modified="2005-10-11" version="1">MTFVVTENCIKCKYMDCVEVCPVDCFYEGDNMLVINPDECIDCGVCEPECPAEAIKPDSEPDLENWLKLNAEYSAVWPNITIKRDAPADAKTFDGVAGKLEQYFSANPGQGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNW0</accession>
    <name>Q3SNW0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2778</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2778"/>
    <dbReference type="EnsemblBacteria" id="ABA06031">
        <property type="protein sequence ID" value="ABA06031"/>
        <property type="gene ID" value="Nwi_2778"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2778"/>
    <dbReference type="eggNOG" id="COG2968">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080344_4_0_5"/>
    <dbReference type="OMA" id="LPNGPHI"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007497">
        <property type="entry name" value="DUF541"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04402">
        <property type="entry name" value="SIMPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229065" evidence="2">
        <location>
            <begin position="31"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="240" mass="24774" checksum="912CFB70E1B5CFDB" modified="2005-10-11" version="1">MRTVMQNRIPLAIAIAIATTTALIPLAAQADTPPPMISVTGEATISTPPDLAQIEAGVTTDAKTAREASEANNAAMSKVLAALKSAGIPEKDFQTSRLSLQPQYTNPNRSSPSVLSGYRATNRVAVKLRDISKLAGVIDTVTAAGANDIGGINFAVSNASKLLDDARAEAVADARRKAEIYAKAAGVSLGAPLSISEHGSSAPVMYRRAAMPMAAEAAIAPGEETLRVTVGISWEIKAAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNW6</accession>
    <name>Q3SNW6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NepR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2772</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315970.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2772"/>
    <dbReference type="EnsemblBacteria" id="ABA06025">
        <property type="protein sequence ID" value="ABA06025"/>
        <property type="gene ID" value="Nwi_2772"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2772"/>
    <dbReference type="eggNOG" id="ENOG502ZJVB">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_185315_0_0_5"/>
    <dbReference type="OrthoDB" id="2071140at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041649">
        <property type="entry name" value="NepR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18557">
        <property type="entry name" value="NepR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NepR" evidence="2">
        <location>
            <begin position="18"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="72" mass="7955" checksum="1006B9CAEF95FBAB" modified="2005-10-11" version="1">MKDSKPPLAKGGLNAEIQSRIGHQLRAMYDDVVRQGIPDRFAELIRKLDASEPGTPQVTPRGDLDVKNDGRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNX1</accession>
    <name>Q3SNX1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2767</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315965.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06020">
        <property type="protein sequence ID" value="ABA06020"/>
        <property type="gene ID" value="Nwi_2767"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2767"/>
    <dbReference type="eggNOG" id="COG4575">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2010404_0_0_5"/>
    <dbReference type="OMA" id="HAVKNDI"/>
    <dbReference type="OrthoDB" id="1787032at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010279">
        <property type="entry name" value="YqjD/ElaB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35893">
        <property type="entry name" value="PTHR35893"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="122" mass="13038" checksum="5D21DF96069CA8F9" modified="2005-10-11" version="1">MASDGENAIKNLTDKATYDRLEKDLSAVKNEISALADQVSDALSGLTGTARKQARRGYRQAKANVDSVLSDVSERGNAALDAAQSAVTTIEETLEDTIQQRPLATVALAMGLGFLIGVTWRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="138" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNY1</accession>
    <name>Q3SNY1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Sulphate transport system permease protein 1</fullName>
            <ecNumber evidence="10">3.6.3.25</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_2757</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in beta-(1--&gt;2)glucan export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.25" evidence="10"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315955.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2757"/>
    <dbReference type="EnsemblBacteria" id="ABA06010">
        <property type="protein sequence ID" value="ABA06010"/>
        <property type="gene ID" value="Nwi_2757"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2757"/>
    <dbReference type="eggNOG" id="COG1118">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_5"/>
    <dbReference type="OMA" id="HLGWEIQ"/>
    <dbReference type="OrthoDB" id="1220708at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015419">
        <property type="term" value="F:ABC-type sulfate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102025">
        <property type="term" value="F:ABC-type thiosulfate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03296">
        <property type="entry name" value="ABC_CysA_sulfate_importer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005666">
        <property type="entry name" value="Sulph_transpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024765">
        <property type="entry name" value="TOBE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12857">
        <property type="entry name" value="TOBE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="SSF50331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00968">
        <property type="entry name" value="3a0106s01"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0764">Sulfate transport</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="3"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA06010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="349" mass="38512" checksum="ABBEB412FC345992" modified="2005-10-11" version="1">MSIELKNIVKRFGAFSALNNVDLTIGDGELLALLGPSGSGKTTLLRIIAGLDWPDSGSVSFDGENALARGAGERQVGFVFQHYALFRHMTVFENVAFGLRVQPRAIRKDEASIRARVKELLDLVQLDWLADRYPSQLSGGQRQRIALARALATEPRILLLDEPFGALDAKVRKELRRWLRSLHEEIHVTSVFVTHDQEEALEVANRVVVMDKGRIEQIGKPGEVYNSPATAFVHGFIGESIVLPVDIQDGVIRLGGTPLNIAAECGASGPSKLFVRRHDMQISPPGWGGLEGTVRRVRSFGPIQRADIALSDGQGETVIEIDAPRDRELRDGEVVGLQPRRYRIFADRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNZ4</accession>
    <name>Q3SNZ4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">OmpA/MotB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2744</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315942.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2744"/>
    <dbReference type="EnsemblBacteria" id="ABA05997">
        <property type="protein sequence ID" value="ABA05997"/>
        <property type="gene ID" value="Nwi_2744"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2744"/>
    <dbReference type="eggNOG" id="COG1360">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016890_14_3_5"/>
    <dbReference type="OMA" id="YVSMENK"/>
    <dbReference type="OrthoDB" id="1619550at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07185">
        <property type="entry name" value="OmpA_C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006665">
        <property type="entry name" value="OmpA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036737">
        <property type="entry name" value="OmpA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00691">
        <property type="entry name" value="OmpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103088">
        <property type="entry name" value="SSF103088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51123">
        <property type="entry name" value="OMPA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="22"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="OmpA-like" evidence="4">
        <location>
            <begin position="216"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="132"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="342" mass="37778" checksum="91A933B857EDF759" modified="2005-10-11" version="1">MALARGRRNESGFNYWPGFVDALSTLVLAIVFLLTVFLVTQFFLSQEVTGKDKALEQLNARIAQLTDLLSLEKLGKVSLGDEVSQLRAGLASAEAERDRIKGLYDGLAGAGNDAAGRDSQLNKALDSEKQISSRALAQVEVLTQQISALRRQLAALENALDASEKKDKESQNRIADLGQRLNVALAKRVQELSRYRSEFFGRLRAILGNRPDIRIVGDRFVFQSEVFFDTAQATLLPEGKAELDKVAGALTDLDKQIPSEIAWVLRVDGHTDVRPIKSPVFKSNWDLSAARAISVVQYLASLGVPPQRLVAAGFGEFQPLDPDATEEAYKRNRRIELKLTER</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP22</accession>
    <name>Q3SP22_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Diguanylate cyclase/phosphodiesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2716</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2716"/>
    <dbReference type="EnsemblBacteria" id="ABA05969">
        <property type="protein sequence ID" value="ABA05969"/>
        <property type="gene ID" value="Nwi_2716"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2716"/>
    <dbReference type="eggNOG" id="COG5001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_70_49_5"/>
    <dbReference type="OMA" id="ALNTMSH"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="3">
        <location>
            <begin position="371"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="EAL" evidence="2">
        <location>
            <begin position="512"/>
            <end position="762"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="778" mass="86230" checksum="94A0958911BEFC0E" modified="2005-10-11" version="1">MRAQPMQLVDHHRDTDTMSPAIYAALVGSLFQNPAPMFAGAACAAIAAVMTALKTGNHLLWPCALLLVLIGLLRSFDMAKYKNRRSDLTVSEAGRWEARYQIGAVLYATALGAWCAIALLMTDDAVAHMICISVTTGYVAAGAGRAYGLPWIFHLQILLTCGPMAGAMALRGDPYYFGMALLTVLYFLGLERITTRLQQIFLRALIAREREAALAGQFDTALNNMPHGLCMFGADGRLGVMNYRFNEMMHLADDFVHRGVGAVDVIAACVTAGTMSPENGRAIVSEIENSQAGAIVTADVDLERGRALSWTFQPMAGGGTVILVEDITERRKAEAKINHLARFDSLTELPNRLSFRDEIERLLASLNGESRLSALLFIDLDQFKQINDTLGHPCGDRLLCMVADRLRGMLRPEDFVARFGGDEFVVFQQNIRSPEEASALARRIVERLAERYEVDRHLVEIGASIGIALTSPGITADNLLKHADMALYRAKADGRGTFCFFRGEMAETVESRRVLELDLRKALAREEFELFYQPLVHLKSGRITTCEALLRWNHPTRGMVSPMDIIPVAEEMGLILDLGRWILRKACMECMRWPEAVSVAVNFSSQQFHQRDILSEVRYALEVSGLPAHRLEIEITESSLLRNTEWTHDALRQLHSAGVKISLDDFGTGYSSLSYLHNFPLQKVKIDRSFLEGIDTDRPLMLLRGVARLSADLGMAVVVEGIETTEQLELVSLDGTVTEAQGYLFSRPVSGVRIRELLDASHGRYLHDNNVKAVRSIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP30</accession>
    <name>Q3SP30_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cell wall hydrolase, SleB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2708</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315907.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2708"/>
    <dbReference type="EnsemblBacteria" id="ABA05961">
        <property type="protein sequence ID" value="ABA05961"/>
        <property type="gene ID" value="Nwi_2708"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2708"/>
    <dbReference type="eggNOG" id="COG3773">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_565792_0_0_5"/>
    <dbReference type="OMA" id="STHYHAY"/>
    <dbReference type="OrthoDB" id="682655at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011105">
        <property type="entry name" value="Cell_wall_hydrolase_SleB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042047">
        <property type="entry name" value="SleB_dom1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07486">
        <property type="entry name" value="Hydrolase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydrolase_2" evidence="2">
        <location>
            <begin position="307"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="460" mass="49628" checksum="57FE7395E44DDDD6" modified="2005-10-11" version="1">MAVLRNQPKSVRLALFGSGLCFFALVPNEIGYQDIASLLARQPGVAERWQKRVSASAVSSIQVATFNFGRPVGTSSPRAATLLLASLDEGVGAVVRNRLARPPQRYSASDFPSVNRALKGDRLALAGPETPPVAPTNAAPQDPSTSNASVLGAKTAAGHGEPATLDPELAEALNAPPLPQYGASLSLEGQPSGDLEDDSAVEGSQSSTEGFSIKTASLYFGNSALGVVNEGLERWRPGEEPVIVMPAPDPDMKVALPPRRDSARAGETVVGKGEVKTPAERLGLLDDKKRARQEKCLAEAVYFEARGEAVRGQIAVAQVVMNRVFSGYYPTTVCGVVYQNADRRLACQFTFACDGIRDVVTEPDMWDRARRIAKATLDGRLWLPEVGKSTHYHAYWVHPSWVREMKKMYRTGVHTFYRPRAWGDGRDAPSWGATAQTAEISARLAEAARSSAELDSGRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="129" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP49</accession>
    <name>Q3SP49_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">4-hydroxy-3-methylbut-2-enyl diphosphate reductase</fullName>
            <shortName evidence="5">HMBPP reductase</shortName>
            <ecNumber evidence="5">1.17.7.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ispH</name>
        <name evidence="6" type="ordered locus">Nwi_2689</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>H2O + isopentenyl diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin] = (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:24488"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:128753"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="1.17.7.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>dimethylallyl diphosphate + H2O + 2 oxidized [2Fe-2S]-[ferredoxin] = (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:24825"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:128753"/>
            <dbReference type="EC" id="1.17.7.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="5">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the IspH family.</text>
    </comment>
    <dbReference type="EC" id="1.17.7.4" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2689"/>
    <dbReference type="EnsemblBacteria" id="ABA05942">
        <property type="protein sequence ID" value="ABA05942"/>
        <property type="gene ID" value="Nwi_2689"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2689"/>
    <dbReference type="eggNOG" id="COG0761">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027486_1_0_5"/>
    <dbReference type="OMA" id="HNKYVVD"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00097"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00059">
        <property type="reaction ID" value="UER00105"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051745">
        <property type="term" value="F:4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050992">
        <property type="term" value="P:dimethylallyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13944">
        <property type="entry name" value="lytB_ispH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00191">
        <property type="entry name" value="IspH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003451">
        <property type="entry name" value="LytB/IspH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30426">
        <property type="entry name" value="PTHR30426"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02401">
        <property type="entry name" value="LYTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00216">
        <property type="entry name" value="ispH_lytB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3 5" id="KW-0408">Iron</keyword>
    <keyword evidence="4 5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="240"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="329" mass="35714" checksum="9DC2C550F17285E7" modified="2005-10-11" version="1">MGDSVPDRLMNAKPKLKIVLCSPRGFCAGVVRAIDTVERALTLYGAPVYVRHEIVHNRYVVDSLRAKGAIFVEELAEIPDDTTAPVVFSAHGVPKSVPADAQRRNFFSLDATCPLVTKVHREAAIHFKRGHEILLIGHSHHPEVVGTVGQLPAGAVTLIETAEDAEKFTPKDADNLAFVTQTTLSIDDTAEIVAVLKQRFPNIHGPHKEDICYATTNRQLAVKKVAPVVDALIVVGAPNSSNSQRLREVAEREGCKVSVLAQRAGDLDWSMFEGIKSLGITAGASAPEVIVEEIMDAFAARYELEVETVSAAEENEFFPLPRSLRPDAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP62</accession>
    <name>Q3SP62_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2676</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05929">
        <property type="protein sequence ID" value="ABA05929"/>
        <property type="gene ID" value="Nwi_2676"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2676"/>
    <dbReference type="eggNOG" id="ENOG502ZV0W">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1169943_0_0_5"/>
    <dbReference type="OMA" id="SQEYYWP"/>
    <dbReference type="OrthoDB" id="1593362at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="217" mass="23550" checksum="66436B1DD63258E0" modified="2005-10-11" version="1">MTRTMTILALACCVVTGVALAEIFGHGNVLMVRFVAAPSPQSAEGEPLESAANRERKADRLPVMLQAGTPDAEGALPEALRPAVATDSPFDAEILPSTPPIEEPPLPRPRPKLASQLVQKDYSLLSDMQIAALKGRLRLTPGQEPYWPAVERALRETAQKIHERRSAPGKQPALRAGEIEQIKVAAQPLLTRLREDQKREVRALARIIGLEAVASLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP75</accession>
    <name>Q3SP75_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Isochorismatase hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2663</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315866.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2663"/>
    <dbReference type="EnsemblBacteria" id="ABA05916">
        <property type="protein sequence ID" value="ABA05916"/>
        <property type="gene ID" value="Nwi_2663"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2663"/>
    <dbReference type="eggNOG" id="COG1335">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091983_0_0_5"/>
    <dbReference type="OMA" id="HLCVDMQ"/>
    <dbReference type="OrthoDB" id="1442962at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="SSF52499"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase" evidence="1">
        <location>
            <begin position="13"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="202" mass="22492" checksum="1D8800474E1F414A" modified="2005-10-11" version="1">MGKLKAAPGPHSIHLCIDMQRLFDEGGPWPAPWMARVLPTVERLVTHAPERTVFTRFIPPQKPEKATGMWRAYYAKWPRVTRNVMDLSLLRLVPELETFAPPAHVIDKPAYSAFTASALQSYLAEMGVDTLLITGSETDVCVLATVLSAVDLGYRTIVVKDGLCSSSDETHDASLRLYAQRFNVQVELTDAAELIDSWRPDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPL1</accession>
    <name>Q3SPL1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative outer-membrane immunogenic protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2527</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315731.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2527"/>
    <dbReference type="EnsemblBacteria" id="ABA05780">
        <property type="protein sequence ID" value="ABA05780"/>
        <property type="gene ID" value="Nwi_2527"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2527"/>
    <dbReference type="eggNOG" id="COG3637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037100_0_1_5"/>
    <dbReference type="OMA" id="WTAKIEY"/>
    <dbReference type="OrthoDB" id="2018542at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027385">
        <property type="entry name" value="OMP_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13505">
        <property type="entry name" value="OMP_b-brl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229165" evidence="1">
        <location>
            <begin position="24"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="OMP_b-brl" evidence="2">
        <location>
            <begin position="22"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="206" mass="21877" checksum="78F8E42D956ABB9C" modified="2005-10-11" version="1">MTRITIAALAAMASTALIGAAHAADFSYRAPYTVNQPLDAFSWAGPYLGGNLGYAWGSVQNSLTRPDGVFGGVQAGFNWQSGPVVFGLEGDIQANAANDTFAAWKFSNPWFGTVRGRIGYAFNNVLVYGTGGLAFGKLRAETFGLSESHTSAGWTVGVGAEVGIYQNWTAKVEYLYVDLSSSSFTITGAPHSYQSGMVRAGINYHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPM4</accession>
    <name>Q3SPM4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pyrroline-5-carboxylate reductase</fullName>
            <shortName evidence="2">P5C reductase</shortName>
            <shortName evidence="2">P5CR</shortName>
            <ecNumber evidence="2">1.5.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">PCA reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">proC</name>
        <name evidence="5" type="ordered locus">Nwi_2514</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the reduction of 1-pyrroline-5-carboxylate (PCA) to L-proline.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-proline + NAD(+) = 1-pyrroline-5-carboxylate + 2 H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:14105"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15893"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-proline + NADP(+) = 1-pyrroline-5-carboxylate + 2 H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:14109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15893"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the pyrroline-5-carboxylate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.2" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2514"/>
    <dbReference type="EnsemblBacteria" id="ABA05767">
        <property type="protein sequence ID" value="ABA05767"/>
        <property type="gene ID" value="Nwi_2514"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2514"/>
    <dbReference type="eggNOG" id="COG0345">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042344_0_2_5"/>
    <dbReference type="OMA" id="AKQTCLG"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00361"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004735">
        <property type="term" value="F:pyrroline-5-carboxylate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01925">
        <property type="entry name" value="P5C_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029036">
        <property type="entry name" value="P5CR_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000304">
        <property type="entry name" value="Pyrroline-COOH_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645">
        <property type="entry name" value="PTHR11645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14748">
        <property type="entry name" value="P5CR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000193">
        <property type="entry name" value="Pyrrol-5-carb_rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00112">
        <property type="entry name" value="proC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F420_oxidored" evidence="3">
        <location>
            <begin position="42"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="P5CR_dimer" evidence="4">
        <location>
            <begin position="194"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="301" mass="30891" checksum="B5B326A612713337" modified="2005-10-11" version="1">MAGSKSGNDADLRDRVVSVRQVRNPAMVESRASLANISGAVVLAGAGKMGNALLTGWLARGLDPKRLVVIEPYPSDDVRALASRGIRLNPTEETGEAAALVIAVKPQTFGDTGPALRPLLGRSTLVVSIMAGTTIAKLDMVCGGNIVRAMPNTPAAIGRGITVAIAATSVNDKQHAMADALLRATGAVEWIDDEALMDAVTAVSGSGPAYVFLLAEELARAGVSAGLPADLAAKLARETVAGSGELLHRSDLDAATLRRNVTSPGGTTAAALDILMGEQGLKDLMARAVAAATKRSRELAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPW3</accession>
    <name>Q3SPW3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Quinolinate synthase</fullName>
            <ecNumber evidence="2 9">2.5.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">nadA</name>
        <name evidence="10" type="ordered locus">Nwi_2424</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>dihydroxyacetone phosphate + iminosuccinate = H(+) + 2 H2O + phosphate + quinolinate</text>
            <dbReference type="Rhea" id="RHEA:25888"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29959"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
            <dbReference type="EC" id="2.5.1.72"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from iminoaspartate: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the quinolinate synthase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.72" evidence="2 9"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315636.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2424"/>
    <dbReference type="EnsemblBacteria" id="ABA05678">
        <property type="protein sequence ID" value="ABA05678"/>
        <property type="gene ID" value="Nwi_2424"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2424"/>
    <dbReference type="eggNOG" id="COG0379">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047382_0_0_5"/>
    <dbReference type="OMA" id="FFLPDKC"/>
    <dbReference type="OrthoDB" id="613347at2"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00327"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008987">
        <property type="term" value="F:quinolinate synthetase A activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10800">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00568">
        <property type="entry name" value="NadA_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003473">
        <property type="entry name" value="NadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036094">
        <property type="entry name" value="NadA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023066">
        <property type="entry name" value="Quinolinate_synth_type2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30573">
        <property type="entry name" value="PTHR30573"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02445">
        <property type="entry name" value="NadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142754">
        <property type="entry name" value="SSF142754"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00550">
        <property type="entry name" value="nadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0408">Iron</keyword>
    <keyword evidence="8 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Iminoaspartate binding" evidence="9">
        <location>
            <begin position="139"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Iminoaspartate binding" evidence="9">
        <location>
            <begin position="224"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA05678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="327" mass="35603" checksum="4434E7E1F38D8DE6" modified="2005-10-11" version="1">MPSVLELRPSARIYPKVRHAIPRIEWPAFADDVDAIIELKKRRNAVVLAHNYQTPEIFHGVADIVGDSLALARKATEVEADVIVLAGVHFMAETAKLLNPSKTVLIPDTGAGCSLADSITPEDVRLLRKTYPGVPIVTYVNTSAAVKAESDICCTSGNAKAVVESLGVERVIMLPDEYLAKNIAAETHVKVIAWAGHCEVHERFSADEVRQLRDNHPGVVVLAHPECPPEVVAEADFSGSTAAMASYVATRQPPRVVLLTECSMSDNVAADHPGLEFIRPCNLCPHMKRITLGNIRHALETMRHEVTIDPSIAERARLAVERMLSVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ11</accession>
    <name>Q3SQ11_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2376</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2376"/>
    <dbReference type="EnsemblBacteria" id="ABA05630">
        <property type="protein sequence ID" value="ABA05630"/>
        <property type="gene ID" value="Nwi_2376"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2376"/>
    <dbReference type="HOGENOM" id="CLU_163997_0_0_5"/>
    <dbReference type="OMA" id="NVRKNME"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="123" mass="13287" checksum="433F9A7A97047305" modified="2005-10-11" version="1">MAFSAVNRNSGATALAFRRIMTILGLMTKSPPSPAQLARNERMRTAAIEGAKARADIEARDVAIRKNMERLRALRLAKEAEEAAKPKPAKPVKASARGKKAAPKQTAKLSDFLASQRSTGRTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ55</accession>
    <name>Q3SQ55_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2332</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2332"/>
    <dbReference type="EnsemblBacteria" id="ABA05586">
        <property type="protein sequence ID" value="ABA05586"/>
        <property type="gene ID" value="Nwi_2332"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2332"/>
    <dbReference type="eggNOG" id="COG2801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2059502_0_0_5"/>
    <dbReference type="OMA" id="WCLASAQ"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="124" mass="13694" checksum="37844858B1F12921" modified="2005-10-11" version="1">MASRISMGPRREVLAAVVGRYRSAGRADKGRILDELCAVTGWHRKHAVRALAHVAISSEARRQRRPTYGAAIRDALVALWEASDRICGKRLKVMIPTLLPSLERHGGSSSTRPIERWCLASAQP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ65</accession>
    <name>HMUV_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hemin import ATP-binding protein HmuV</fullName>
            <ecNumber evidence="1">7.6.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hmuV</name>
        <name type="ordered locus">Nwi_2322</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the ABC transporter complex HmuTUV involved in hemin import. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of two ATP-binding proteins (HmuV), two transmembrane proteins (HmuU) and a solute-binding protein (HmuT).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. Heme (hemin) importer (TC 3.A.1.14.5) family.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315541.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SQ65"/>
    <dbReference type="SMR" id="Q3SQ65"/>
    <dbReference type="STRING" id="323098.Nwi_2322"/>
    <dbReference type="EnsemblBacteria" id="ABA05576">
        <property type="protein sequence ID" value="ABA05576"/>
        <property type="gene ID" value="Nwi_2322"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2322"/>
    <dbReference type="eggNOG" id="COG4559">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_11_5"/>
    <dbReference type="OMA" id="YPRITWI"/>
    <dbReference type="OrthoDB" id="1752365at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51261">
        <property type="entry name" value="HMUV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000269603" description="Hemin import ATP-binding protein HmuV">
        <location>
            <begin position="1"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="5"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01718"/>
        </source>
    </evidence>
    <sequence length="269" mass="28730" checksum="E48D0FAF7DDD4BF3" modified="2005-10-11" version="1">MRPIIETHSVTMRINGATLVDGIDLSVGSGEMVAIVGPNGAGKSTLLRLLSGDLQPTQGRIELKQADLRSYSAAQLATHRATLSQHVNVTFPFTVEEIVRMGAGDMAIAAAHPLVESAMDEVAIRPLRSRQLPTLSGGEQQRTHFARVLVQLACGEARHGPGLLLLDEPTSSLDLRHQIELVEIAGRRARNGTAVIAILHDLNLAVRFASRIIVIHRGAIVADGPPAQTITDGLIRKVFEVCADIQYHGDGSPVLLPQAMKSIGPGNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ92</accession>
    <name>Q3SQ92_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GCN5-related N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2295</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2295"/>
    <dbReference type="EnsemblBacteria" id="ABA05549">
        <property type="protein sequence ID" value="ABA05549"/>
        <property type="gene ID" value="Nwi_2295"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2295"/>
    <dbReference type="eggNOG" id="COG1247">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_19_8_5"/>
    <dbReference type="OMA" id="AEFWIGV"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="17"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="175" mass="20156" checksum="5D9E714DCB39D013" modified="2005-10-11" version="1">MQAHDRLTELPEKRMEITIRAMRSEDAADIFEIVNQHSFRFWTLALPYEPFDKVQKWLEPQSPRDLHLSAELDGRVVGASALRPFYGRRAHAAEFWIGVHEDHSRKGIGSRLLGAMIDAADNWLNIKRIEMTVFTDNVSALALYNKFGFEIEGTHKAATFRDGMFVDAYCMARLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQA9</accession>
    <name>Q3SQA9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 10">Phosphoenolpyruvate carboxylase</fullName>
            <shortName evidence="10">PEPC</shortName>
            <shortName evidence="10">PEPCase</shortName>
            <ecNumber evidence="5 10">4.1.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ppc</name>
        <name evidence="13" type="ordered locus">Nwi_2278</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 10">Forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>oxaloacetate + phosphate = hydrogencarbonate + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:28370"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.1.1.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the PEPCase type 1 family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.31" evidence="5 10"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315499.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2278"/>
    <dbReference type="EnsemblBacteria" id="ABA05532">
        <property type="protein sequence ID" value="ABA05532"/>
        <property type="gene ID" value="Nwi_2278"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2278"/>
    <dbReference type="eggNOG" id="COG2352">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006557_2_0_5"/>
    <dbReference type="OMA" id="PWVFGWT"/>
    <dbReference type="OrthoDB" id="398146at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008964">
        <property type="term" value="F:phosphoenolpyruvate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015977">
        <property type="term" value="P:carbon fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006107">
        <property type="term" value="P:oxaloacetate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00595">
        <property type="entry name" value="PEPcase_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021135">
        <property type="entry name" value="PEP_COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022805">
        <property type="entry name" value="PEP_COase_bac/pln-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018129">
        <property type="entry name" value="PEP_COase_Lys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033129">
        <property type="entry name" value="PEPCASE_His_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30523">
        <property type="entry name" value="PTHR30523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00311">
        <property type="entry name" value="PEPcase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00150">
        <property type="entry name" value="PEPCARBXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00781">
        <property type="entry name" value="PEPCASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00393">
        <property type="entry name" value="PEPCASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0120">Carbon dioxide fixation</keyword>
    <keyword evidence="8 10 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="10 11">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <feature type="active site" evidence="10 12">
        <location>
            <position position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA05532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="928" mass="103516" checksum="086D73A411023D76" modified="2005-10-11" version="1">MSSLMPLPRAGQPESDAADLDARLRDDIRLLGRILGDTVRDREGPEAFDLVERIRQTSIRFHRDDDKPARRELEAILDGMSTDETVLVVRAFSYFSHLANIAEDQNNIRQMRSQTLAGGARHQGRLAHTLACARKAGFSAAELRDFFDSAVVSPVLTAHPTEVRRKSTIDREMEVAALLDRRERMQLTPDEYEASEEQLRRAVLTLWQTDLLRRTKLTVLDEVANGLSFYDYTFLREVPRLHCMLEDRLHDPEESSAANEIASFLRIGSWIGGDRDGNPFVTADVMRGTLHWQVSRILRHYLDELHELGRELSLAADLTDVSPELSSLAERSADTSPHRRSEPYRLAVSGIYARLSATAVKLKADTLRPPYGAAPPYASAAEFSADLDILHHSLTANNSEVIARGRLRKLRRAADCFGFHLACLDLRQNSTVHERTIAELLAAASPGTSYLTLDEEARIVLLLRELRSARPLTSPFITYSEETQGELAVFHAAAEAHGTLGPDAIPQCIVSMTEGISDLLEVALLLKEADLVDPTGHSAINIVPLFETIEDLQACAGIMDRLLSLPEYRALVDSRGGLQEVMLGYSDSNKDGGFVTSGWELYKAEIGLVEVFERHKVRLRLFHGRGGSVGRGGGPSYDAILAQPGGAVNGQIRITEQGEIISSKYSNAEVGRSNLEILAAATLEASLLQPRQSAPSLEYLDAMESLSSLAYKAYRNLVFETPGFEDYFWASTVINEISTLNIGSRPASRKKTRRIEDLRAIPWVFSWAQCRVMLPGWYGFGSAVEAWIESHPDKGMAFLQELYGEWPFFRMLLSNMDMVLAKSSIAIASRYADLVPDVELREAIFGRIRREWHLTIDKLLAIMGQERLLAGNPLLERSIRNRFPYLDPLNHVQVELLKDHRAQCEDEQVLRGIQLTINGISAGLRNSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQE1</accession>
    <name>Q3SQE1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Peptidase S8 and S53, subtilisin, kexin, sedolisin</fullName>
            <ecNumber evidence="7">3.4.21.66</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2246</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.4.21.66" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2246"/>
    <dbReference type="EnsemblBacteria" id="ABA05500">
        <property type="protein sequence ID" value="ABA05500"/>
        <property type="gene ID" value="Nwi_2246"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2246"/>
    <dbReference type="eggNOG" id="COG1404">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029776_0_0_5"/>
    <dbReference type="OMA" id="RDQNSGP"/>
    <dbReference type="OrthoDB" id="923655at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="SSF52743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229259" evidence="5">
        <location>
            <begin position="34"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S8" evidence="6">
        <location>
            <begin position="318"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="114"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="588"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="600"/>
            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="627" mass="64709" checksum="92C5D85EA5624475" modified="2005-10-11" version="1">MRQRMTGRMKWSGALFALTGLCWLASDASPAHAQGNMQGFRGSPSFGMQGRAPMPSPGGGMTGFPDRGGGPRGMGSGLGSGLGLGLGLGYGLMLGATGASSRAPYVEVPDRAIERDENVNRRPSRPQPRKTVSRERKPRKPVIGVTASGISYIRYAPNTFPACARGNGAMSGDCNGRPFVVDLGGNGPPPRDQNSGPRRNAGQAAVTRNYLPGEIVAETETMGEAQLTALARRHWLQLIASQDFPLTGSTVSLFRIAGRRPVEDVQRELRADEAVRSAQPNFRYILQDQNTEAVTEGDPAQYALAKLRLPEAHRLANGEGVIVAVIDSAIDVDHPELAGSIAASFDALDGKGKPHVHGTGVAGAIVAHARLMGSAPSARILAIRAFGVTQGAAESTSFTILRSLDHAAAYGARIINMSFAGPKDALVSRGIAAAAEKGIVLVAASGNAGPKSPPLHPAAAAQVIAVSATDADDRLFPASNRGSHVAVAAPGVDIFLPAPDRKYQMTSGTSFAAAYISGLAALILERRPALKPAEVRAILMTSARDLGAPGRDDLFGAGQADAYRAVQSVPDNAGPAADKEMPAIAVSGPATVVSGSADEAAGETASSSEQQPAPAGLESRGADPSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQF1</accession>
    <name>Q3SQF1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Heterodimeric methylmalonyl-CoA mutase small subunit</fullName>
            <ecNumber evidence="7">5.4.99.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2236</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="5.4.99.2" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315458.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2236"/>
    <dbReference type="EnsemblBacteria" id="ABA05490">
        <property type="protein sequence ID" value="ABA05490"/>
        <property type="gene ID" value="Nwi_2236"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2236"/>
    <dbReference type="eggNOG" id="COG1884">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009523_6_0_5"/>
    <dbReference type="OMA" id="WYVERLT"/>
    <dbReference type="OrthoDB" id="720283at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004494">
        <property type="term" value="F:methylmalonyl-CoA mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016176">
        <property type="entry name" value="Cbl-dep_enz_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036724">
        <property type="entry name" value="Cobalamin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006099">
        <property type="entry name" value="MeMalonylCoA_mutase_a/b_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01642">
        <property type="entry name" value="MM_CoA_mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51703">
        <property type="entry name" value="SSF51703"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52242">
        <property type="entry name" value="SSF52242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0846">Cobalamin</keyword>
    <keyword evidence="4" id="KW-0170">Cobalt</keyword>
    <keyword evidence="3 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="METMALONYL_COA_MUTASE" evidence="6">
        <location>
            <begin position="67"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="532"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="639" mass="67730" checksum="EC1D07CFDC682DAA" modified="2005-10-11" version="1">MPAAPHEPIDPPLAADFAPALAADWRKLVDGVLKGAPFERLVGKTYDGLRIEPIYTRAEGAPPIAGRAAATPWRIMQRIDHPEPAQANAQALHDLDNGAGGLTLVFAGANGARGFGLPPTAKAVARVLDGVFLDAGITIELHIAPQSLGVAFQIADHIRTKGIDPAACDIHFGLDPLGACATSGSSTNEWAEIVAAATETAKTLAARGFKGPFLVADGRVIHDAGGSEAQELAFVLASAVTYLRALESAGVVLDDARGMIHARLSTDADQFLTLAKFRALRLLWARVEQACGLAPRPLLIAAETSWRMLTRRDPYMNMLRATVAAFSAGLGGADSVCVLPHTQALGLPDGFARRMARNTQLILLEEANLAKVADPAAGSGGIETLTRELCQKSWALFQDTERAGGAFAALEQNLIQRGVAATRDARQTSVAKRRDVLTGISEFPDLHETPIDVLDVEPVTLAPSGEAKISFEALTPIRLAAPFEQLRDHSDDWLKATGARPKILLATLGVPAGFAARAAFARSFFEAGGIEAVDPPSPRSRQGDVEGAARREDENALAEAFKASGARMVCLCSSDEVYAEQAVLAARAFHAAGAGHIYLAGRPNELEPVLRQAGVRDFIFAGNDALAVLNEVWQRLEQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQF4</accession>
    <name>Q3SQF4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dihydropteroate synthase</fullName>
            <shortName evidence="1">DHPS</shortName>
            <ecNumber evidence="1">2.5.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Dihydropteroate pyrophosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2233</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of para-aminobenzoate (pABA) with 6-hydroxymethyl-7,8-dihydropterin diphosphate (DHPt-PP) to form 7,8-dihydropteroate (H2Pte), the immediate precursor of folate derivatives.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DHPS family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.15" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315455.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2233"/>
    <dbReference type="EnsemblBacteria" id="ABA05487">
        <property type="protein sequence ID" value="ABA05487"/>
        <property type="gene ID" value="Nwi_2233"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2233"/>
    <dbReference type="eggNOG" id="COG0294">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008023_1_3_5"/>
    <dbReference type="OMA" id="FSIDTYH"/>
    <dbReference type="OrthoDB" id="1275854at2"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00156"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004156">
        <property type="term" value="F:dihydropteroate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00739">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045031">
        <property type="entry name" value="DHP_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006390">
        <property type="entry name" value="DHP_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000489">
        <property type="entry name" value="Pterin-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20941">
        <property type="entry name" value="PTHR20941"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00809">
        <property type="entry name" value="Pterin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="SSF51717"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01496">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00792">
        <property type="entry name" value="DHPS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00793">
        <property type="entry name" value="DHPS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50972">
        <property type="entry name" value="PTERIN_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pterin-binding" evidence="2">
        <location>
            <begin position="29"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU361205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="292" mass="31102" checksum="26136FA2E2BB1E02" modified="2005-10-11" version="1">MTSADSMSRAVTDDARHPALEALLSRPCPAVMGILNITPDSFSDGGQFMTPDRAVAQARRMIADGADIIDIGAESTRPYGAQPISRDEEMRRLEPVLAAVVALGISVSIDSMKSPVVEWALGNAAAIANDVWGLQRDPDMARVVAARNAPVVVMHNRDKPDGTLDIMRDITAFFERSLKIAAQAGIPASRIILDPGIGFGKTPEQSMIALARLGELQTFGLPVLVGASRKRFISMVTPSEPQQRLGGSLAAHLIAARSGARIIRTHDVAETVQALRVAHAIETATQPGSFEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQV5</accession>
    <name>Q3SQV5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, XRE family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2078</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315315.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2078"/>
    <dbReference type="EnsemblBacteria" id="ABA05336">
        <property type="protein sequence ID" value="ABA05336"/>
        <property type="gene ID" value="Nwi_2078"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2078"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153788_1_0_5"/>
    <dbReference type="OMA" id="KSTMGTK"/>
    <dbReference type="OrthoDB" id="2063007at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="2">
        <location>
            <begin position="17"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="126" mass="13573" checksum="9EC22483BB124960" modified="2005-10-11" version="1">MSSPKSKTALERLGRDLRAARLSRRVAVADLAVRAGTSASTVARLEKGDPGVGIGTLADVLVALGLIERLADLVDIRKDDLGLALTSERVPQRGRSFATTLRKQERQNDKPKRNEPDTVDPDGVAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQW2</accession>
    <name>Q3SQW2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Luciferase-like protein</fullName>
            <ecNumber evidence="3">1.14.14.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2071</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.14.14.3" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315308.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2071"/>
    <dbReference type="EnsemblBacteria" id="ABA05329">
        <property type="protein sequence ID" value="ABA05329"/>
        <property type="gene ID" value="Nwi_2071"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2071"/>
    <dbReference type="eggNOG" id="COG2141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_3_0_5"/>
    <dbReference type="OMA" id="YVGDPEY"/>
    <dbReference type="OrthoDB" id="1434838at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047646">
        <property type="term" value="F:alkanal monooxygenase (FMN-linked) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_luciferase" evidence="2">
        <location>
            <begin position="41"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="360" mass="39240" checksum="968AF2C05C95430C" modified="2005-10-11" version="1">MTNTVYAPTASSDVERSGPNTNDAPQSFKLGLSGCGGGLESISTSHILDLAEKIEALGFTGIWLNEEHFQGSIVEVEGRRCHSPLILASAILARTTRLRVGFSVLLAALHNPIRLAEEIATLDVLSNGRVDVGISRGNNPRYLNAYGVNPEGTADHFKDMLTFLPRAWSVGRLPFGDAAHSIEPKPIQSPHPPIYVGTNTNETAAWATKSGHKIICHGIISMPNQRRLMRAYVDAGGNPAEVPFGRFVYVSETDASARKELWPTIEKLTTRLKGFGLFRRDGVIDEAGLEPDNFYREMVITGSPETCAEKFMALHGELGVTYLNALSAFFGFLPLDHLERSLDLMGSVLRPKLERALAAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQZ9</accession>
    <name>Q3SQZ9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2033</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the ArsC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315276.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2033"/>
    <dbReference type="EnsemblBacteria" id="ABA05292">
        <property type="protein sequence ID" value="ABA05292"/>
        <property type="gene ID" value="Nwi_2033"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2033"/>
    <dbReference type="eggNOG" id="COG1393">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116644_2_1_5"/>
    <dbReference type="OMA" id="FGIKNCD"/>
    <dbReference type="OrthoDB" id="1606021at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006660">
        <property type="entry name" value="Arsenate_reductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006504">
        <property type="entry name" value="Tscrpt_reg_Spx/MgsR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30041">
        <property type="entry name" value="PTHR30041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03960">
        <property type="entry name" value="ArsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01617">
        <property type="entry name" value="arsC_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51353">
        <property type="entry name" value="ARSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="121" mass="13636" checksum="E1F7430B11A15830" modified="2005-10-11" version="1">MTTTIYGIKNCDTMKKARAWLDDHGVAYDFHDYKTAGIDGVRLKGWAAKLGWEALLNRAGTTFRKLPDAKKAGLTEARALALMMEQPSMIKRPVLEAGDKLLVGFKPDIYEEQLRPLKVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR39</accession>
    <name>Q3SR39_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1993</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315239.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1993"/>
    <dbReference type="EnsemblBacteria" id="ABA05252">
        <property type="protein sequence ID" value="ABA05252"/>
        <property type="gene ID" value="Nwi_1993"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1993"/>
    <dbReference type="eggNOG" id="ENOG5032WAV">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158704_0_0_5"/>
    <dbReference type="OMA" id="IPEDETC"/>
    <dbReference type="OrthoDB" id="1897003at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="97" mass="10687" checksum="34DCC4F62BD2F3AE" modified="2005-10-11" version="1">MAKYVGLDQDTHGITALGRTVLDARVFGLIPETQDCAGWDAGQMQALMSKVNNLWDQYGNVPSALPPELADRHNRVYSEAIARAKARGWDAELDDND</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR57</accession>
    <name>Q3SR57_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nitric oxide reductase activation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1975</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315221.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1975"/>
    <dbReference type="EnsemblBacteria" id="ABA05234">
        <property type="protein sequence ID" value="ABA05234"/>
        <property type="gene ID" value="Nwi_1975"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1975"/>
    <dbReference type="eggNOG" id="COG4548">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020675_0_0_5"/>
    <dbReference type="OMA" id="IHGFCSD"/>
    <dbReference type="OrthoDB" id="39518at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="SSF53300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="1">
        <location>
            <begin position="596"/>
            <end position="784"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="786" mass="89275" checksum="E7423B2C92FCC9F0" modified="2005-10-11" version="1">MAIHLHEFQELLDELPGRAQELLRASWNEAARAISPRGLSNYIKGAVALHELGRGEDLVVSFIQSMPEVGRAIGEEVLPDLVNFLLGMASKTSGQVLALIASTAPLAAHRVGDAELFRQYLNVLEIMLAQAPRGLRPMLENLDRLLSQLTLGGLRRWVQWGAQAYKADFEGQAAYFGLQSADAMAVLQQERKGTLFVDIQRRLNIYMRAIWGRDFFLRPTAGDYESREGLKPYIEHYVIHVADAYDDYKPEGDATALERIVSALELYRAAVNHCAAHLVFTQTPLSMQQLLPVQMALIELFEDARVEELAIRKFPGMRQAWLALHPVFDYSAQPRTVGELFDRLARALLDPDSTDPDPFVREGVAAFRAQSDLESNQVSWNLGVDLSHRLFELDLPTYNKVTDALSAVYRDDNRAVWFLEEYDEAQVMAATWETRQQVRKKVNVMEMVNEVDVEFAGDDAQEVWELPTEFYLDQENVSINSLEGRPPISEPFHYPEWDYQIQLERPNWVTLLEYHPVMGELDVIEGIILEHKPVISRLKFLIESMVPQGMQRIRNVEDGDEIDINAAVRAMIDIRIGQQHDPRIMMRYKRALRDLAVLVLLDMSESSNDKVRGHDYTVMDLTRAATVLLAGALHRIGDPFAIHGFCSDGRHDVHYHRFKDFDQPYDDVVKARLAGMKGRLSTRMGAALRHAAHYLALQPSSKRVVFVVTDGEPADNDVTDPQYLRHDTKAAVEELGRQGITVYALSLDPHADQYVSRIFGMKNFTVIDQAERLPEKLPMLYMSLTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR68</accession>
    <name>Q3SR68_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1963</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1963"/>
    <dbReference type="EnsemblBacteria" id="ABA05223">
        <property type="protein sequence ID" value="ABA05223"/>
        <property type="gene ID" value="Nwi_1963"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1963"/>
    <dbReference type="eggNOG" id="COG1100">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112884_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="212" mass="22945" checksum="51FED37E39DF5A57" modified="2005-10-11" version="1">MALPIISADQRLAEQRGVKGTIFGKSGIGKTSLLWTLDPATTLFIDLEAGDLAIEGWSGDSVRPRTWAECRDFAVFIGGPNPALRDDQVYSDAHYAAVCERFGDPAALDRYHTVFIDSITVAGRLCFQWCKGQPEAFSEKTGKPDVRGAYGLHGREMIAWLTHLQHTRAKCPSSDELRLFRGHGNGGSGSSGFEVRRPAGDAASVFGGWSGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR70</accession>
    <name>Q3SR70_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1961</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315212.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1961"/>
    <dbReference type="EnsemblBacteria" id="ABA05221">
        <property type="protein sequence ID" value="ABA05221"/>
        <property type="gene ID" value="Nwi_1961"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1961"/>
    <dbReference type="eggNOG" id="ENOG5031AXS">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1658744_0_0_5"/>
    <dbReference type="OMA" id="ESRYTNW"/>
    <dbReference type="OrthoDB" id="1731868at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="162" mass="17845" checksum="BA56C16E7E2D1FD4" modified="2005-10-11" version="1">MTISNRISLDELRHMAVGDIVALPAEQLALLQDAAADALRRARTVCDWLDGAVALKYGDRARATRQAAGKDTGTIRFDDGAVTVIADLPKRVDWDQDKLAALVERIRAEGDDPTEYVDVAIKVPERKFAAWPSHIRSAFEDARTVRTGKPSFRLSLNTEVTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRC5</accession>
    <name>Q3SRC5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Antibiotic biosynthesis monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1906</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315162.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05166">
        <property type="protein sequence ID" value="ABA05166"/>
        <property type="gene ID" value="Nwi_1906"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1906"/>
    <dbReference type="eggNOG" id="COG2329">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_156590_2_0_5"/>
    <dbReference type="OMA" id="DHMVHFR"/>
    <dbReference type="OrthoDB" id="1909149at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007138">
        <property type="entry name" value="ABM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03992">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51725">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABM" evidence="1">
        <location>
            <begin position="2"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="100" mass="11664" checksum="1D3FFD87AA321668" modified="2005-10-11" version="1">MITEIAQIEIKPGSEEDFEAAVSQAQTIFKRCKGWRSCELHRSVERPSRYRLLIKWETLENHVVDFRESSDFTEWRELVGPFFASSPEVEHTETVTAFSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRD6</accession>
    <name>Q3SRD6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1895</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315151.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05155">
        <property type="protein sequence ID" value="ABA05155"/>
        <property type="gene ID" value="Nwi_1895"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1895"/>
    <dbReference type="eggNOG" id="ENOG50315W8">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_156392_0_0_5"/>
    <dbReference type="OMA" id="LPYACGW"/>
    <dbReference type="OrthoDB" id="2063999at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229268" evidence="1">
        <location>
            <begin position="22"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="103" mass="11499" checksum="95C5DDB7FB0DC6F2" modified="2005-10-11" version="1">MLRAISLAAIGVAMMGATGSAADLPAHSRIGKIFAEPTPPPARVAQEKDYPESVLYRFRVPPPVPGYYGRPNDFYYRPYYEGRPVISLYFDRLPYACGLYGYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRD8</accession>
    <name>Q3SRD8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Peptidase T, Metallo peptidase, MEROPS family M20B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_1893</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20B family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315149.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1893"/>
    <dbReference type="EnsemblBacteria" id="ABA05153">
        <property type="protein sequence ID" value="ABA05153"/>
        <property type="gene ID" value="Nwi_1893"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1893"/>
    <dbReference type="eggNOG" id="COG2195">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053676_0_0_5"/>
    <dbReference type="OMA" id="GHNFHGK"/>
    <dbReference type="OrthoDB" id="1015417at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045148">
        <property type="term" value="F:tripeptide aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006518">
        <property type="term" value="P:peptide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010161">
        <property type="entry name" value="Peptidase_M20B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42994:SF1">
        <property type="entry name" value="PTHR42994:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037215">
        <property type="entry name" value="Peptidase_M20B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01882">
        <property type="entry name" value="peptidase-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00759">
        <property type="entry name" value="ARGE_DAPE_CPG2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="M20_dimer" evidence="7">
        <location>
            <begin position="222"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="388"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR037215-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR037215-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA05153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="417" mass="45553" checksum="C918A77BCF3B563A" modified="2005-10-11" version="1">MTAPMTFHHTVTERFLRYVVIDTQSDPASSSYPSTEKQKDLGRLLVSELQELGIADAHLDSHGYVYATLPANSSKQVPVICFCSHMDTSPDCSGANVKPQIVRNYRGGDIHLRGDPSQIIRAADHPALADQVGNDIITSDGTTLLGADNKAGVAEIMDAVQVLLANPEVRHGTIKILFTPDEEIGRGVDKVDLKKLGADFAYTVDGETAGYIEDETFSADAALITIKGVSAHPGFAKGKMEHAIKIASRIVERLPRNACSPETTDGREGFLHPTDISGTLESATLSLIVRDFTETGLTEKKMLLEDIIRDIMADFPHSTCRLEIQPQYRNMKEVVDRHPEVVDNAMEAIRRAGLEPVKGSVRGGTDGSRLSFMGLPCPNIFAGEHAFHSRTEWVSVQDMEAAVRTIVHLAAIWEERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRI3</accession>
    <name>Q3SRI3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase</fullName>
            <shortName evidence="7">UDP-N-acetylglucosamine acyltransferase</shortName>
            <ecNumber evidence="7">2.3.1.129</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">lpxA</name>
        <name evidence="9" type="ordered locus">Nwi_1848</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a (3R)-hydroxyacyl-[ACP] + UDP-N-acetyl-alpha-D-glucosamine = a UDP-3-O-[(3R)-3-hydroxyacyl]-N-acetyl-alpha-D-glucosamine + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:67812"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="ChEBI" id="CHEBI:173225"/>
            <dbReference type="EC" id="2.3.1.129"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 1/6.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the transferase hexapeptide repeat family. LpxA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.129" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315104.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1848"/>
    <dbReference type="EnsemblBacteria" id="ABA05108">
        <property type="protein sequence ID" value="ABA05108"/>
        <property type="gene ID" value="Nwi_1848"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1848"/>
    <dbReference type="eggNOG" id="COG1043">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061249_0_0_5"/>
    <dbReference type="OMA" id="ECVTINR"/>
    <dbReference type="OrthoDB" id="971098at2"/>
    <dbReference type="UniPathway" id="UPA00359">
        <property type="reaction ID" value="UER00477"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008780">
        <property type="term" value="F:acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009245">
        <property type="term" value="P:lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03351">
        <property type="entry name" value="LbH_UDP-GlcNAc_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00387">
        <property type="entry name" value="LpxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029098">
        <property type="entry name" value="Acetyltransf_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037157">
        <property type="entry name" value="Acetyltransf_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010137">
        <property type="entry name" value="Lipid_A_LpxA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43480">
        <property type="entry name" value="PTHR43480"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13720">
        <property type="entry name" value="Acetyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000456">
        <property type="entry name" value="UDP-GlcNAc_acltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01852">
        <property type="entry name" value="lipid_A_lpxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7" id="KW-0441">Lipid A biosynthesis</keyword>
    <keyword evidence="1 7" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Acetyltransf_11" evidence="8">
        <location>
            <begin position="176"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="268" mass="28570" checksum="91E056C34620C9EA" modified="2005-10-11" version="1">MSRIDPTARIEDGAVIGESVEIGPFCTVGPHVVLGPNCRLISHVSVAGHTTIGAGCTIYPFAALGGAPQDMGYGNEPTRLEIGEGCTIRESVTMNVGSPKDVGVTRIGARGFFMSYSHVGHDCQVGDDVVFANSATLGGHCKIGDFVYIGGLSAVHQFARIGRQAMIGGLTGIRGDVIPYGFVNGQHGHLEGLNVVGMRRRKFTRERLARVRTFYQELFYGPGLFADRLQRVQSRASDDPAIAEILTFIGADKHRPLCLPEGGANKSA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRJ3</accession>
    <name>TRPC_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Indole-3-glycerol phosphate synthase</fullName>
            <shortName evidence="1">IGPS</shortName>
            <ecNumber evidence="1">4.1.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trpC</name>
        <name type="ordered locus">Nwi_1838</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H(+) = (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:23476"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="EC" id="4.1.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TrpC family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.48" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315094.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRJ3"/>
    <dbReference type="SMR" id="Q3SRJ3"/>
    <dbReference type="STRING" id="323098.Nwi_1838"/>
    <dbReference type="PRIDE" id="Q3SRJ3"/>
    <dbReference type="EnsemblBacteria" id="ABA05098">
        <property type="protein sequence ID" value="ABA05098"/>
        <property type="gene ID" value="Nwi_1838"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1838"/>
    <dbReference type="eggNOG" id="COG0134">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034247_2_0_5"/>
    <dbReference type="OMA" id="RGPHDLI"/>
    <dbReference type="OrthoDB" id="1789381at2"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00043"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004425">
        <property type="term" value="F:indole-3-glycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00331">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00134_B">
        <property type="entry name" value="IGPS_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045186">
        <property type="entry name" value="Indole-3-glycerol_P_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013798">
        <property type="entry name" value="Indole-3-glycerol_P_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001468">
        <property type="entry name" value="Indole-3-GlycerolPSynthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22854">
        <property type="entry name" value="PTHR22854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00218">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00614">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0210">Decarboxylase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="chain" id="PRO_1000018512" description="Indole-3-glycerol phosphate synthase">
        <location>
            <begin position="1"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00134"/>
        </source>
    </evidence>
    <sequence length="264" mass="28634" checksum="428B326CDC0232CA" modified="2005-10-11" version="1">MADILTRIETYKREEIASAKRARPLGEVTAAAKAAPPPRGFTDTIRAKLARGDYALIAEIKKASPSKGLIRADFDPPSLARAYEAGGAACLSVLTDAPSFQGHLDFMVAARAATSLPVLRKDFIFDTYQVVEARAHGADCILIIMAALDDDAAKDIEATALDLGMDVLLEIHNREELDRALKLRSPMIGVNNRNLRTFETTLETSEGLAPYIPKERLMVGESGIFTPADLARLERVGISTFLVGESLMRQADVTAATRTLLKHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRV2</accession>
    <name>Q3SRV2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1728</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04989">
        <property type="protein sequence ID" value="ABA04989"/>
        <property type="gene ID" value="Nwi_1728"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1728"/>
    <dbReference type="HOGENOM" id="CLU_2317408_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="99" mass="10849" checksum="464CE0931FEECB81" modified="2005-10-11" version="1">MMISRSNTRNDILSLETAATAARGGFACLFSSSDEYEQALIAERHARGRYTSTRRLVDDGLDCSVGHDRPLDTASAKRGTARGSLARVFWLTVGHCFPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRW0</accession>
    <name>Q3SRW0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribonuclease E</fullName>
            <shortName evidence="5">RNase E</shortName>
            <ecNumber evidence="5">3.1.26.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rne</name>
        <name evidence="7" type="ordered locus">Nwi_1720</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions.</text>
            <dbReference type="EC" id="3.1.26.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="5">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 2 Zn(2+) ions per homotetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer formed by a dimer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell inner membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RNase E/G family. RNase E subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.12" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1720"/>
    <dbReference type="EnsemblBacteria" id="ABA04981">
        <property type="protein sequence ID" value="ABA04981"/>
        <property type="gene ID" value="Nwi_1720"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1720"/>
    <dbReference type="eggNOG" id="COG1530">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3170">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003468_5_0_5"/>
    <dbReference type="OMA" id="RFLIEVW"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008995">
        <property type="term" value="F:ribonuclease E activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00970">
        <property type="entry name" value="RNase_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019307">
        <property type="entry name" value="RNA-bd_AU-1/RNase_E/G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028878">
        <property type="entry name" value="RNase_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004659">
        <property type="entry name" value="RNase_E/G"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30001">
        <property type="entry name" value="PTHR30001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10150">
        <property type="entry name" value="RNase_E_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00757">
        <property type="entry name" value="RNaseEG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2 5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="5" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="118"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Required for zinc-mediated homotetramerization and catalytic activity" evidence="5">
        <location>
            <begin position="693"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="800"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="886"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="118"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="174"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="252"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="313"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="339"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="806"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="825"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="927"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="966"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="592"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="635"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="693"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="696"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1059" mass="117679" checksum="A12ABCA7BA4C9770" modified="2005-10-11" version="1">MGRPRATRLPCHAPSASKSFKMPNKPNKMLIDATHPEETRVVVVRGNRVEEFDFESAQRKQLRGNIYLAKVTRVEPSLQAAFIEYGGNRHGFLAFSEIHPDYYQIPVADRQALIEADERAHREAEEESEDRSSRRRSRHRNARRRDHGERVQSAVVENGEHQVAGADGTQSDPSHSDPSHDEFAAGDHLGQQHDVQDHDQPNDPDHHHLDDDDHSHESGSISLYGSDYETVADHLPESPDFAFAADSDNGDHEQDENQEDTGDDQVAASSEEDLPSIAPHDAVGSEPSADDGARDDQVEPAISSSPNEDHALGVHASGDHDHDEPHAEEFHGDEVRVEDRADDDGDDDEEEEQVESVGGDDALEEVPERAFRPRRQYKIQEVIKRRQVMLVQVVKEERGNKGAALTTYLSLAGRYAVLMPNTARGGGISRKITSAQDRSRLKEVVQDLDVPEGMGVILRTAGAARTKPEIKRDFEYLIRMWETVRDMTLRSQAPTLVYEEGSLIKRSLRDLYSKEIDEILVAGEAGYHEARGFMKMLMPSNARAVKQYRDCQPLFSRMGVESQLDAMFLPTVQLRSGGYIVINQTEALVSIDVNSGRSTREHHIKDTALKTNLEAAEEVARQLRLRDLAGLIVIDFIDMDEKRSNRAVERKLSECLRHDRARIQVGRISHFGLLEMSRQRIRASMLESSTEPCPQCGGSGHVRSVPSVALQLLRSLEEVLMKGATHNLIVRTRINVALYVLNQKREHLRALEHSFRVGLSVVADPAIAGQQSFVIDRGEQVHTLEAAKALLATQLVTYPPQAEDVQDDEPTLDTETETEADDAEVRVDEQAGGETEGDGRKRRRRRRRERSSETNDSQVSQAVEVPDSVQIAAEFAAARFMIDDVENEGEDSSAQESAAPADQPADERRPRRRGRRGGRRRRGTNGANPVDSASDELNVTSVSEAVDAVADLDSVSLAPEARSEPAESETQPQQQSEESASSPAETGNGTVPEPEKAVCRRSTVREKVSFTSQEEPPSVPVVQGEPEESTPEAQQPPQASDETRPRRAGWWSRRFGGGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRX6</accession>
    <name>Q3SRX6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1704</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314963.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04965">
        <property type="protein sequence ID" value="ABA04965"/>
        <property type="gene ID" value="Nwi_1704"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1704"/>
    <dbReference type="eggNOG" id="ENOG50348SR">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_905791_0_0_5"/>
    <dbReference type="OMA" id="VSIMNPE"/>
    <dbReference type="OrthoDB" id="1173662at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="306" mass="32138" checksum="8F00148EA2FD5872" modified="2005-10-11" version="1">MNWGWVTSLDQFWRSPAFPMWLTLAGAVFFAFIVFLTLIRAEKSVANGVLTIITLLAIGVAAAVALRTFEADGSRSLDSKNQSVRTNIAIPALSCIDGLAGEVVESACEKALFGTPDAVAAAVSYVASEINRLRSFGAAAADGSIVAPELEVLRRTIERDRYGLVAHVLVARDHCQPSDCAAFQSLTDHGRIAANMKEQLYENLVVRYASSWNAPAVAQAAAPGTPPVPALASEPTGNPTTMDFPTAESIPPVSIMTPEPAAAPPASSSAAAQAVPKPRPAARKPHAPKPHAPPPVRLAPPPTPQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRX9</accession>
    <name>Q3SRX9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1701</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1701"/>
    <dbReference type="EnsemblBacteria" id="ABA04962">
        <property type="protein sequence ID" value="ABA04962"/>
        <property type="gene ID" value="Nwi_1701"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1701"/>
    <dbReference type="eggNOG" id="COG2304">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1128360_0_0_5"/>
    <dbReference type="OMA" id="VFMFQEG"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="SSF53300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="254" mass="27124" checksum="63D870A875F63807" modified="2005-10-11" version="1">MAYDPEPPKRSPNPTICRSPPAGGSAPLPDVKSSSSDIAAFVAKARSMSPYASDGRGRLLFALDATMSRQPTWDMACTLQADMFHEAVGIGSLDIRLVYYRGLNECRSSGWIPDSAKLARLMSAIDCRGGNTQIGKVLSHARTEAVASGIKAVVFVGDAMEESVDDLCAKAGELGLLKVPVFMFQEGHDAIAEQAFREIARLTGGAWCRFDPGAASQLRELLRAAAAYAVGGREALRRLSTTVTSATRLLGQMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS16</accession>
    <name>Q3SS16_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Iron-regulated ABC transporter membrane component SufB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1664</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0051 (ycf24) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314924.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1664"/>
    <dbReference type="EnsemblBacteria" id="ABA04925">
        <property type="protein sequence ID" value="ABA04925"/>
        <property type="gene ID" value="Nwi_1664"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1664"/>
    <dbReference type="eggNOG" id="COG0719">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026231_0_0_5"/>
    <dbReference type="OMA" id="YYSAPKQ"/>
    <dbReference type="OrthoDB" id="635721at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010231">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000825">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037284">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045595">
        <property type="entry name" value="SufBD_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01458">
        <property type="entry name" value="SUFBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19295">
        <property type="entry name" value="SufBD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101960">
        <property type="entry name" value="SSF101960"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01980">
        <property type="entry name" value="sufB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SufBD_N" evidence="2">
        <location>
            <begin position="156"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="493" mass="55173" checksum="051B4D7B67E6F295" modified="2005-10-11" version="1">MAAVQETVDRVRQIDVDQYRYGFETIIESEKAPKGLSEDTIRFISAKKNEPAWMLEWRLEAYRRWLTMQEPTWARVDYPKIDYQDLYYYSAPKPKKEIASLDEIDPEILKTYEKLGIPLREVEVLEGVVRPEGERKVAVDAVFDSVSVATTFQEELKKAGVIFMPISHAIQQHPELVKQYLGSVVPTSDNFFATLNSAVFSDGSFVYVPPGVRCPMELSTYFRINERNTGQFERTLIIADKGSYVSYLEGCTAPQRDENQLHAAVVELVTLDDAEIKYSTVQNWYPGNSEGKGGIFNFVTKRGDCRGANSKISWTQVETGSAITWKYPSCILRGDNSRGEFYSIAISNGYQQVDSGTKMLHLGKNTTSRIISKGIAAGKSQNTYRGLVTAHRKATNARNFTACDSLLIGDKCGAHTVPYIEAKNTSALFEHEATTSKISEDVLFYCVQRGLSQEEAVGLVVNGFVRDVLQQLPMEFAVEAQKLISISLEGSVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS30</accession>
    <name>Q3SS30_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Poly(R)-hydroxyalkanoic acid synthase, class I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_1650</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1650"/>
    <dbReference type="ESTHER" id="nitwn-q3ss30">
        <property type="family name" value="PHA_synth_I"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04911">
        <property type="protein sequence ID" value="ABA04911"/>
        <property type="gene ID" value="Nwi_1650"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1650"/>
    <dbReference type="eggNOG" id="COG3243">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017387_1_0_5"/>
    <dbReference type="OMA" id="YIYGSRE"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042619">
        <property type="term" value="P:poly-hydroxybutyrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010963">
        <property type="entry name" value="PHA_synth_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010941">
        <property type="entry name" value="PhaC_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07167">
        <property type="entry name" value="PhaC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01838">
        <property type="entry name" value="PHA_synth_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PhaC_N" evidence="6">
        <location>
            <begin position="119"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="5">
        <location>
            <begin position="326"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="609" mass="67967" checksum="F3AE028885A78307" modified="2005-10-11" version="1">MSKLQDASMSEVSAEAKPTKGFDSDAFAMNLASAMENGGRALAAYLKAREQKQTPDNPPTEFAELIKTVSAVSEYWLSDQARAVDLQARLGKSYLELWGYAARRLAGEEVEPAIEPDPRDKRFADPEWRSNLLFNFLMQAYLLTTKWTNELVRDAHGLDPHTRKKAEFYITQLTNAAAPSNFVMTNPEVLRETFASNADNLARGMKMLAEDIEAGRGVLRIRQSDSAGLAVGVNLATTPGKVIYQNELMQLIQYSPSTENVLRTPLLIVPPWINKFYILDLTPEKSYIRWCVDQGITVFVISWVNPDGHLGEKTFEDYMKQGPLAAMDAIEKATGETKVHTAGYCVGGTLLAATLAWLANKRRQRVRSVTFFAAQVDFTHAGDLLVFADEDQISALERDMKRSGVLEGSKMAMTFNMLRSNDLIWPYVVSNYLKGKAPSAFDLLHWNSDATRMPAANHSFYLRNCYLENRLSTGSMVLDNTLLDLSRVRVPVYNLATRDDHIAPAESVLYGSRFFGGPVRFVLAGSGHIAGVINPPSRGKYQYWTNDGISDKTLAQWMQDAQEHKGSWWPDWLAWLRNIDGKEVPAREVGTGALSPIEDAPGSYVRVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS33</accession>
    <name>Q3SS33_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 16">Homoserine dehydrogenase</fullName>
            <ecNumber evidence="6 16">1.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">Nwi_1647</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-homoserine + NAD(+) = H(+) + L-aspartate 4-semialdehyde + NADH</text>
            <dbReference type="Rhea" id="RHEA:15757"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.1.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>L-homoserine + NADP(+) = H(+) + L-aspartate 4-semialdehyde + NADPH</text>
            <dbReference type="Rhea" id="RHEA:15761"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.1.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 16">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 3/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 16">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 17">Belongs to the homoserine dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.3" evidence="6 16"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314907.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="ABA04908"/>
        <property type="gene ID" value="Nwi_1647"/>
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        <property type="taxonomic scope" value="Bacteria"/>
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    <dbReference type="OrthoDB" id="1464088at2"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00063"/>
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        <property type="reaction ID" value="UER00465"/>
    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:homoserine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:isoleucine biosynthetic process"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ACT_dom"/>
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        <property type="entry name" value="Asp/hSer_DH_NAD-bd"/>
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        <property type="entry name" value="HDH"/>
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    </dbReference>
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        <property type="entry name" value="HDH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00742">
        <property type="entry name" value="Homoserine_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03447">
        <property type="entry name" value="NAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000098">
        <property type="entry name" value="Homoser_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01042">
        <property type="entry name" value="HOMOSER_DHGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="16" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="9 16" id="KW-0412">Isoleucine biosynthesis</keyword>
    <keyword evidence="13 16" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="11 15 16" id="KW-0521">NADP</keyword>
    <keyword evidence="12 16 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 16" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="ACT" evidence="18">
        <location>
            <begin position="351"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="14">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="10"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
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        <location>
            <position position="107"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
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        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005056"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005062"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000098-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000098-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU004171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ABA04908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="440" mass="46381" checksum="968F45FE8452A65F" modified="2005-10-11" version="1">MVAPLKVGIAGLGTVGSAVVRLIEQQRGALTARCGRGVRVVAVAARSKTRKRDVDLTDIAWARDALALANDPGIDCFVELMGGSDDPAHSAIEAALKSGKSVVTANKALIAKHGLRLASLAEQHGGALNYEAAVGAAIPVIKTLREGLAGTTINRVYGILNGTCNYILTRMEREGLSFAECLKDAQRLGYAEADPSFDVDGHDTAQKLSILASLAFGTRIAQNEVYVEGISSIAPEDLRAAEELGYRVKLLGVAVRTSKGIEQRVHPTMVPKTSSIAQVMGVTNAVTIDGDGIPPITLVGPGAGGAATASAVVADIADVASGTRALPFGRHSTKLRETKKAPMERHEGGYYIRLMARDFAGTAATIATRLAEQKISIESIVQRHPNGKLDTDESDTPISPVPVLLITYATSEDAVHRALHAVRADKVITGRPQVIRIEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS50</accession>
    <name>Q3SS50_NITWN</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ordered locus">Nwi_1630</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
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    </dbReference>
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    </dbReference>
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        <property type="protein sequence ID" value="ABA04891"/>
        <property type="gene ID" value="Nwi_1630"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1630"/>
    <dbReference type="HOGENOM" id="CLU_2602463_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="79" mass="8545" checksum="3695C1C2664D2CB3" modified="2005-10-11" version="1">MMQITRIEPAAPDRPGSPTLIALFDVETPNAVLRNCKLLESGTGECFVLAPAGLKFWSDSALRDEICEAALDALDEIEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS75</accession>
    <name>Q3SS75_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Carboxyl transferase</fullName>
            <ecNumber evidence="4">6.4.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1605</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.4.1.3" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ABA04866">
        <property type="protein sequence ID" value="ABA04866"/>
        <property type="gene ID" value="Nwi_1605"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1605"/>
    <dbReference type="eggNOG" id="COG4799">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018822_0_1_5"/>
    <dbReference type="OMA" id="AYLPIMS"/>
    <dbReference type="OrthoDB" id="123223at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004658">
        <property type="term" value="F:propionyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045190">
        <property type="entry name" value="MCCB/AccD1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22855">
        <property type="entry name" value="PTHR22855"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="2">
        <location>
            <begin position="21"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="3">
        <location>
            <begin position="277"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="534" mass="57050" checksum="BDD05E9A0D3BE3D4" modified="2005-10-11" version="1">MALRSGVDPTSSDFARNVEAMKTLVGELREKLAGVAGGGGEAAVARHVSRGKMLARDRIDLLLDPGTAFLELSPLAANGMYGGDVHSASIITGVGRVAGRECVIVANDATIKGGTYYPMTVKKHLRAQDIARQNNLPCIYMVDSGGAFLPHQDEIFPDERHFGRIFYNQASMSAAGIPQVAIVMGSCTAGGAYVPAMSDESIIVRNQGTIFLGGPPLVKAATGEVVTAEELGGADVHSRQSGVTDHYARNDADAIGIAHRIVSTLKPPQRAALNMREPREPLFAREEIYGVVPADGRKPFDVRDVIARIVDGSEFDEFKKLYGQTLVCGFAHIWGYPVGVVANNGILFSESSLKGAHFIELCCQRGIPLVFLQNITGFMVGKKYEAGGIARDGAKLVTAVATAGVPKFTVVIGGSYGAGNYGMCGRAFSPRFLWMWPNARISVMGGEQAAMVLSTLRRENIEAKGGTWSAEDESEFQAPIRAQYESQGNPYYATARLWDDGVIDPADTRLVLGLGLSAAANAPIEPTTFGLFRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS98</accession>
    <name>Q3SS98_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1582</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314849.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04843">
        <property type="protein sequence ID" value="ABA04843"/>
        <property type="gene ID" value="Nwi_1582"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1582"/>
    <dbReference type="eggNOG" id="ENOG503141W">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_182288_0_0_5"/>
    <dbReference type="OMA" id="VVRARKC"/>
    <dbReference type="OrthoDB" id="1954859at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="83" mass="8987" checksum="6E7B5EA85DA39C00" modified="2005-10-11" version="1">MLEYRGPLLLLMIVGTLTVAATAAYASQVSRETDVVNLRLGERILVDDGSCPAGHIKEVSGTKLTPAGVVRARKCIPRIRLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSA6</accession>
    <name>Q3SSA6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1574</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314841.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1574"/>
    <dbReference type="EnsemblBacteria" id="ABA04835">
        <property type="protein sequence ID" value="ABA04835"/>
        <property type="gene ID" value="Nwi_1574"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1574"/>
    <dbReference type="eggNOG" id="ENOG5032YT2">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_177114_0_0_5"/>
    <dbReference type="OMA" id="HTMWVEL"/>
    <dbReference type="OrthoDB" id="1922535at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018841">
        <property type="entry name" value="DUF2442"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10387">
        <property type="entry name" value="DUF2442"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="82" mass="9160" checksum="220589E443D0F715" modified="2005-10-11" version="1">MNISASSVRFDEHTMWVELTDGRTLGVPLAWFPRLLRATPAQRDKVEISRVGLHWDDLDEDISIAGILAGRGDVTHRPDHAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSD4</accession>
    <name>Q3SSD4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_1546</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the N(4)/N(6)-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1546"/>
    <dbReference type="REBASE" id="11321">
        <property type="entry name" value="M.NwiORF1546P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04807">
        <property type="protein sequence ID" value="ABA04807"/>
        <property type="gene ID" value="Nwi_1546"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1546"/>
    <dbReference type="eggNOG" id="COG0863">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1475">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024927_0_0_5"/>
    <dbReference type="OMA" id="GDWDKDK"/>
    <dbReference type="OrthoDB" id="1487464at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006306">
        <property type="term" value="P:DNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002941">
        <property type="entry name" value="DNA_methylase_N4/N6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015840">
        <property type="entry name" value="DNA_MeTrfase_ParB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB/Sulfiredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001091">
        <property type="entry name" value="RM_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01555">
        <property type="entry name" value="N6_N4_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02195">
        <property type="entry name" value="ParBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036758">
        <property type="entry name" value="Aden_M_ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00508">
        <property type="entry name" value="S21N4MTFRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00470">
        <property type="entry name" value="ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="SSF110849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="1 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ParB" evidence="6">
        <location>
            <begin position="39"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="470" mass="52731" checksum="4B2C095D7CD2070C" modified="2005-10-11" version="1">MKMTNTSTKPKRTRKPKAETPRVELEPIGPRAANRVREETWPIDQLRPYDRNAKKHDQAQLDQIRASLRQYGQVHRVLVDEAGIVIAGHGRLEALKQEQFAEVRVLIAVGWEEAEKRKFRLADNQLTMSTGWDEKLLKGEVLELGALGVELDQLGFEPGRIAGLLHEPTAGLTDPDEVPEPPPVPTSRRGDVWILGRHRITNGDSTNPEDVARVLAGRVPHLMVTDPPYGVKYDPAWRQAAGVTSKDAATGKVLNDDRADWREAWELFPGDVAYIWHAGAYCGDVANSLTACRFKIRAHIVWVKQRHVLGRGDYHFQHEPCFYAVKEGADERWHFVPEHEVATYTVRDGERGHYEGGRKQSTVWNIEHVKSETGHGTQKPVEAMKRPIENNSQPGEMVYEPFSGSGTTLIAAEITGRKCFAIELNPLYVDVAVKRWQQFTGLVATLESDGRTFDDVAAERDAERRAAAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSG4</accession>
    <name>Q3SSG4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1516</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1516"/>
    <dbReference type="EnsemblBacteria" id="ABA04777">
        <property type="protein sequence ID" value="ABA04777"/>
        <property type="gene ID" value="Nwi_1516"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1516"/>
    <dbReference type="eggNOG" id="COG2928">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068050_1_1_5"/>
    <dbReference type="OMA" id="PTTGWYT"/>
    <dbReference type="OrthoDB" id="1305123at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007462">
        <property type="entry name" value="COV1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31876">
        <property type="entry name" value="PTHR31876"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04367">
        <property type="entry name" value="DUF502"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="257" mass="27873" checksum="EB93B774EB6FCD07" modified="2005-10-11" version="1">MTRDTPPSGAQGDHSPDMPRGLIARFRNYFLTGLIVAGPVAITLYLTWWFVNWVDNLVRPFVPMAYRPETYLPFILPGSGLIVAVFALTMLGFLTANLIGRTLVDLGEKVLGRMPVVRAIYRGLKQVFETLFSGSGSSLRRVGLVEFPSPGMWSIVLISQVPSANVAARLPSQEEHISVFLPCAPNPTTGFFFYVPKNRVVEIDMSTEEAATLIMSAGVVQPNSDSQKKLAALAEMAQAARVANASNLASTTAAKVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSJ2</accession>
    <name>Q3SSJ2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1488</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04749">
        <property type="protein sequence ID" value="ABA04749"/>
        <property type="gene ID" value="Nwi_1488"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1488"/>
    <dbReference type="HOGENOM" id="CLU_2602462_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="79" mass="8662" checksum="A40E241F3D194A07" modified="2005-10-11" version="1">MNTTPELIQEAQSGLNGMDDHLNSILEYCDLIALLLSAPDIENECPGLHRLVLVMRDHALALDKCQHRAGMAIGRLANP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSQ3</accession>
    <name>Q3SSQ3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Endoribonuclease L-PSP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1427</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314697.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1427"/>
    <dbReference type="EnsemblBacteria" id="ABA04688">
        <property type="protein sequence ID" value="ABA04688"/>
        <property type="gene ID" value="Nwi_1427"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1427"/>
    <dbReference type="eggNOG" id="COG0251">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104845_0_1_5"/>
    <dbReference type="OMA" id="VANYVGF"/>
    <dbReference type="OrthoDB" id="1956412at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02199">
        <property type="entry name" value="YjgF_YER057c_UK114_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013813">
        <property type="entry name" value="Endoribo_LPSP/chorism_mut-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14588">
        <property type="entry name" value="YjgF_endoribonc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="SSF55298"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YjgF_endoribonc" evidence="1">
        <location>
            <begin position="6"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="155" mass="15748" checksum="2082D8C4F9C6A66E" modified="2005-10-11" version="1">MAGVIEQNLAAQGIILHEPASPVANYVGFVRTGNLLFVSGQLCLDPEGNLIAKGKLGADVTVEEGNAAARGCAINLLAQVKAALGDLDKVARVVRLGGFVNSAPDFADGPKVLNGASDLMVAAFGEKGRHSRSTVGVAALPANAAVEIEGLFEVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSU0</accession>
    <name>Q3SSU0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1390</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314662.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1390"/>
    <dbReference type="EnsemblBacteria" id="ABA04651">
        <property type="protein sequence ID" value="ABA04651"/>
        <property type="gene ID" value="Nwi_1390"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1390"/>
    <dbReference type="HOGENOM" id="CLU_2451610_0_0_5"/>
    <dbReference type="OMA" id="WSAVVAY"/>
    <dbReference type="OrthoDB" id="2157415at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="89" mass="10113" checksum="3FC80181E78E6A43" modified="2005-10-11" version="1">MNFEMVFAAALLTDDILAAIHDLHAYSNGGGTAISREKLREIVDKYVTFDEKDFPELRSDVDWSAVVAYWREAVEDMEEAIKGEVPVYK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-11-28" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSV1</accession>
    <name>RL6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplF</name>
        <name type="ordered locus">Nwi_1379</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314653.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSV1"/>
    <dbReference type="SMR" id="Q3SSV1"/>
    <dbReference type="STRING" id="323098.Nwi_1379"/>
    <dbReference type="EnsemblBacteria" id="ABA04640">
        <property type="protein sequence ID" value="ABA04640"/>
        <property type="gene ID" value="Nwi_1379"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1379"/>
    <dbReference type="eggNOG" id="COG0097">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065464_1_2_5"/>
    <dbReference type="OMA" id="KPDPYKG"/>
    <dbReference type="OrthoDB" id="1398618at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.930.12">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01365_B">
        <property type="entry name" value="Ribosomal_L6_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000702">
        <property type="entry name" value="Ribosomal_L6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020040">
        <property type="entry name" value="Ribosomal_L6_a/b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036789">
        <property type="entry name" value="Ribosomal_L6_a/b-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019906">
        <property type="entry name" value="Ribosomal_L6_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002358">
        <property type="entry name" value="Ribosomal_L6_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655">
        <property type="entry name" value="PTHR11655"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655:SF14">
        <property type="entry name" value="PTHR11655:SF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00347">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002162">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00059">
        <property type="entry name" value="RIBOSOMALL6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56053">
        <property type="entry name" value="SSF56053"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03654">
        <property type="entry name" value="L6_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00525">
        <property type="entry name" value="RIBOSOMAL_L6_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000260903" description="50S ribosomal protein L6">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01365"/>
        </source>
    </evidence>
    <sequence length="177" mass="19135" checksum="3E24AD64EAE48713" modified="2005-10-11" version="1">MSRIGKRPVTVPSGVTATVEGQTVKMKGPKGELRFVVHDDVEVKLEDGAVKVAPRYETKRAQALYGTARAQVANLVAGVTKGFEKKLEIIGVGYRAALQGKSLQLALGYSHDVNYAVPEGITIAVPKPTEITITGNDAQRVGQVAAEIRGYRPPEPYKGKGVKYADETIFRKEGKKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSW3</accession>
    <name>RL2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplB</name>
        <name type="ordered locus">Nwi_1367</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314644.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSW3"/>
    <dbReference type="SMR" id="Q3SSW3"/>
    <dbReference type="STRING" id="323098.Nwi_1367"/>
    <dbReference type="EnsemblBacteria" id="ABA04628">
        <property type="protein sequence ID" value="ABA04628"/>
        <property type="gene ID" value="Nwi_1367"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1367"/>
    <dbReference type="eggNOG" id="COG0090">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036235_2_1_5"/>
    <dbReference type="OMA" id="SCIELRP"/>
    <dbReference type="OrthoDB" id="961486at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01320_B">
        <property type="entry name" value="Ribosomal_L2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022666">
        <property type="entry name" value="Rbsml_prot_L2_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002171">
        <property type="entry name" value="Ribosomal_L2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005880">
        <property type="entry name" value="Ribosomal_L2_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022669">
        <property type="entry name" value="Ribosomal_L2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022671">
        <property type="entry name" value="Ribosomal_L2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014726">
        <property type="entry name" value="Ribosomal_L2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691">
        <property type="entry name" value="PTHR13691"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00181">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03947">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002158">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01383">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01382">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01171">
        <property type="entry name" value="rplB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00467">
        <property type="entry name" value="RIBOSOMAL_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000237215" description="50S ribosomal protein L2">
        <location>
            <begin position="1"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="199"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="277" mass="30435" checksum="F84C65971D2F7035" modified="2005-10-11" version="1">MALKTYNPTTPGQRQLVMVDRSALYKGKPVKTLTEGKRGKGGRNNTGRITVRFRGGGHKQAYRNVDFKRGKTDVPAVVERLEYDPNRTAFIALIKYQDGEQAYILAPQRMAVGDTVVAGNYVDVKPGNVMPLGNMPVGTIVHNVEMKIGKGGQLARSAGTYAQIVGRDQDYVILRLNSGEQRLVHGRCRGAIGAVSNPDHMNTSVGKAGRTRWMGRRPHNRGVVMNPIDHPHGGGEGRTSGGRHPVTPWGKPTKGKKTRSNKSTNKFILISRHKRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSZ4</accession>
    <name>Q3SSZ4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1336</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04597">
        <property type="protein sequence ID" value="ABA04597"/>
        <property type="gene ID" value="Nwi_1336"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1336"/>
    <dbReference type="eggNOG" id="ENOG5030XXU">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1863640_0_0_5"/>
    <dbReference type="OMA" id="PFPANYR"/>
    <dbReference type="OrthoDB" id="1568063at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229258" evidence="1">
        <location>
            <begin position="23"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="146" mass="15913" checksum="65014DD82C763F50" modified="2005-10-11" version="1">MLGASAKFVAIAILAASLTACVTDSGDQGRGQGGLVYTDDRGVADQPFPTDYRTQVLAFLRTYLNDPAGIRDAAMAEPAQRTVGGRRRYVSCLRYNAREIDGSYRGVAQRAIVYVDARLDRMIDKTDDICAGAVYAPFPELEKLTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST98</accession>
    <name>Q3ST98_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">(R)-citramalate synthase</fullName>
            <ecNumber evidence="4">2.3.1.182</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_1231</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + H2O + pyruvate = (3R)-citramalate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19045"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30934"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.1.182"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-oxobutanoate from pyruvate: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the alpha-IPM synthase/homocitrate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.182" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314522.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1231"/>
    <dbReference type="EnsemblBacteria" id="ABA04493">
        <property type="protein sequence ID" value="ABA04493"/>
        <property type="gene ID" value="Nwi_1231"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1231"/>
    <dbReference type="eggNOG" id="COG0119">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022158_7_0_5"/>
    <dbReference type="OMA" id="KSWDFHV"/>
    <dbReference type="OrthoDB" id="840579at2"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00066"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043714">
        <property type="term" value="F:(R)-citramalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003852">
        <property type="term" value="F:2-isopropylmalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013709">
        <property type="entry name" value="2-isopropylmalate_synth_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005675">
        <property type="entry name" value="Citramal_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036230">
        <property type="entry name" value="LeuA_allosteric_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43538">
        <property type="entry name" value="PTHR43538"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08502">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00917">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110921">
        <property type="entry name" value="SSF110921"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00977">
        <property type="entry name" value="citramal_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0412">Isoleucine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="10">
        <location>
            <begin position="6"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA04493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="540" mass="58727" checksum="4B0483DC481CA223" modified="2005-10-11" version="1">MSRERLYLFDTTLRDGAQTNGVDFTLQDKQAIAKMLDDLGIDYVEGGYPGANPTDTGFFSHKPAFRHARFTAFGMTRRPGRSASNDPGLAALIEADADAICFVAKSSAYQVRVALETTKDENLASIRDSVKAATAVGREVMVDCEHFFDGYREDPAFALACAKAAYDSGARWVVLCDTNGGAMPNEIEATVREAAKHIPGDHLGIHAHNDTEQAVANSLAAVRAGVRQIQGTLNGLGERCGNANLCSLIPTLKLKKEFSDIFEIGVTGDRLAALTQVSRTLDNMLNRAPNRHAPYVGESAFVTKTGIHASAILKDPATYEHVAPESVGNRRKVLVSDQAGRSNVLAELERANIAFDRNDPKLGRLVREMKEREAAGYAYESANASFDLLARRTLGRVPEYFDVEQYDVNVEQRYNSHGRRVTVAMAVVKVRVDGQPLISAAEGNGPVNALDLALRKDLGKYQKYIDGLKLIDYRVRILNGGTGAVTRVLLESEDEEGERWTTIGVSPNIIDASFQALMDSVFYKLVKSGAPAEGAVPEPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="127" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STA6</accession>
    <name>Q3STA6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Glutathione reductase</fullName>
            <shortName evidence="11">GRase</shortName>
            <ecNumber evidence="11">1.8.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Nwi_1223</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Maintains high levels of reduced glutathione.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>2 glutathione + NADP(+) = glutathione disulfide + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:11740"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="8">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.7" evidence="11"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314514.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1223"/>
    <dbReference type="EnsemblBacteria" id="ABA04485">
        <property type="protein sequence ID" value="ABA04485"/>
        <property type="gene ID" value="Nwi_1223"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1223"/>
    <dbReference type="eggNOG" id="COG1249">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016755_2_1_5"/>
    <dbReference type="OMA" id="CFDYVKP"/>
    <dbReference type="OrthoDB" id="267896at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004362">
        <property type="term" value="F:glutathione-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006324">
        <property type="entry name" value="GSHR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="SSF55424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01424">
        <property type="entry name" value="gluta_reduc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 10" id="KW-0274">FAD</keyword>
    <keyword evidence="3 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 10 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 10" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="13">
        <location>
            <begin position="7"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="Pyr_redox_dim" evidence="12">
        <location>
            <begin position="340"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="437"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="176"/>
            <end position="183"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="9">
        <location>
            <begin position="43"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABA04485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="460" mass="49267" checksum="CE3E35A65E58A412" modified="2005-10-11" version="1">MADFDVDLFVIGGGSGGVRAARIAAGYGARVMVAEEYRMGGTCVIRGCVPKKLLVYGSHIRHDIEDAAGFGWSIPSATFDWPALIANKDREIARLEAAYTATLEKAGVQTVKSRAVFEDAHTLRLTTGETIRSKHVLIATGGRPNQGPAIPGIEHVISSNEAFHLDELPKRIAIQGGGYIALEFACIFAGFGSDVTLIYRGDNILRGFDDDVRAHVRAEMEKSGITILTDCTVERVDRHGDDFTSHLSNGSSVASDQVLFAIGRHPNVANLGLEKAGVAINPENGGIAVDGFSRTNVPHIYAVGDVTHRFNLTPVAIREGHAFADSVFGNKPTRVDHADIPTAVFSQPEVGTVGLTEAQARAEYAVDIYKATFRPLKATLSGRDTRMLMKLVVDATTDRVLGCHIVGDGAAEMTQVVGIAIRMKATKADFDATMALHPTAAEELVTMRTRTAHHARGAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STC0</accession>
    <name>Q3STC0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alpha-1,4-glucan:maltose-1-phosphate maltosyltransferase</fullName>
            <shortName evidence="3">GMPMT</shortName>
            <ecNumber evidence="3">2.4.99.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">(1-&gt;4)-alpha-D-glucan:maltose-1-phosphate alpha-D-maltosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">glgE</name>
        <name evidence="5" type="ordered locus">Nwi_1209</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Maltosyltransferase that uses maltose 1-phosphate (M1P) as the sugar donor to elongate linear or branched alpha-(1-&gt;4)-glucans. Is involved in a branched alpha-glucan biosynthetic pathway from trehalose, together with TreS, Mak and GlgB.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 3">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + alpha-maltose 1-phosphate = [(1-&gt;4)-alpha-D-glucosyl](n+2) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:42692"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:10183"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:63576"/>
            <dbReference type="EC" id="2.4.99.16"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2 3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 13 family. GlgE subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.16" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314500.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1209"/>
    <dbReference type="CAZy" id="GH13">
        <property type="family name" value="Glycoside Hydrolase Family 13"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04471">
        <property type="protein sequence ID" value="ABA04471"/>
        <property type="gene ID" value="Nwi_1209"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1209"/>
    <dbReference type="eggNOG" id="COG0366">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015798_0_0_5"/>
    <dbReference type="OMA" id="RPNFWPN"/>
    <dbReference type="OrthoDB" id="1573900at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030979">
        <property type="term" value="P:alpha-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02124">
        <property type="entry name" value="GlgE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026585">
        <property type="entry name" value="GlgE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021828">
        <property type="entry name" value="GlgE_dom_N/S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="Glyco_hydro_13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357:SF203">
        <property type="entry name" value="PTHR10357:SF203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11896">
        <property type="entry name" value="DUF3416"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aamy" evidence="4">
        <location>
            <begin position="209"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Maltose 1-phosphate binding" evidence="3">
        <location>
            <begin position="528"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="388"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="417"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>alpha-maltose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:63576"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>alpha-maltose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:63576"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="352"/>
        </location>
        <ligand>
            <name>alpha-maltose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:63576"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="389"/>
        </location>
        <ligand>
            <name>alpha-maltose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:63576"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="3">
        <location>
            <position position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="657" mass="74331" checksum="58556B13A2F054D8" modified="2005-10-11" version="1">MSVSTQKNMPLRAGEEGATAGAFHIEDVYPCIDAGRFPVKRLAGDTVDVWADIFRSGHDVIAAQLIWRREQDRDWKRSAMTLHDNDRWFGSFKPAEPGRYVYAIEAWTDEFATWKRDFELKQKAGQDVALDALEGAALLTRAQYGGKEATAIILRQCEDFLHRGDPSSLLVPELTSAMAEGCVRSDLTRSRLYPLTIDRSLAVAGAWYEMVPRSQSTVPGQHGTFRDCMARLPDIAAMGFDVLYFTPIHPIGKANRKGRNNALIAAEGDPGSPYAIGSPEGGHDAIHPDLGSFDDFRELIAACKTHSMEVALDIAVQCSPDHPWLTQHPDWFKRRPDGSMRYAENPPKKYEDIVNPDFGCEDAGSLWNALRDVILFWVNQGVRIFRIDNPHTKPLNFWEWLIHEVQAKDPGVIFLAEAFTRPKVMKGLAKLGFTQSYTYFTWRTRRWEIESYLNELTGYPERDYYRPNFFVSTPDILPYHLQGGEPWMFKSRVALAATLSSNYGIYNGFELLEHEAIPGREEYLNSEKYEIKVRDWDASGNIKSYIAALNRARRANPALLQTRNLIFIPIDNDQIIAFAKQSAEGHNTIVVAITLSRESCEFWLPLDSIRVETADGSGPPVALENLMTCERHAIEWGGVRLRIDPLRDPALLFRCLA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-11-28" modified="2022-05-25" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STC2</accession>
    <name>GLGB_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">1,4-alpha-glucan branching enzyme GlgB</fullName>
            <ecNumber evidence="1">2.4.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Alpha-(1-&gt;4)-glucan branching enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glycogen branching enzyme</fullName>
            <shortName evidence="1">BE</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">glgB</name>
        <name type="ordered locus">Nwi_1207</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Transfers a segment of a (1-&gt;4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.</text>
            <dbReference type="EC" id="2.4.1.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.18" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314498.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3STC2"/>
    <dbReference type="SMR" id="Q3STC2"/>
    <dbReference type="STRING" id="323098.Nwi_1207"/>
    <dbReference type="CAZy" id="CBM48">
        <property type="family name" value="Carbohydrate-Binding Module Family 48"/>
    </dbReference>
    <dbReference type="CAZy" id="GH13">
        <property type="family name" value="Glycoside Hydrolase Family 13"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q3STC2"/>
    <dbReference type="EnsemblBacteria" id="ABA04469">
        <property type="protein sequence ID" value="ABA04469"/>
        <property type="gene ID" value="Nwi_1207"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1207"/>
    <dbReference type="eggNOG" id="COG0296">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004245_3_2_5"/>
    <dbReference type="OMA" id="FGMKWMM"/>
    <dbReference type="OrthoDB" id="227746at2"/>
    <dbReference type="UniPathway" id="UPA00164"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003844">
        <property type="term" value="F:1,4-alpha-glucan branching enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102752">
        <property type="term" value="F:1,4-alpha-glucan branching enzyme activity (using a glucosylated glycogenin as primer for glycogen synthesis)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043169">
        <property type="term" value="F:cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02855">
        <property type="entry name" value="E_set_GBE_prok_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00685">
        <property type="entry name" value="GlgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006048">
        <property type="entry name" value="A-amylase/branching_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037439">
        <property type="entry name" value="Branching_enzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006407">
        <property type="entry name" value="GlgB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044143">
        <property type="entry name" value="GlgB_N_E_set_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="Glyco_hydro_13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004193">
        <property type="entry name" value="Glyco_hydro_13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651">
        <property type="entry name" value="PTHR43651"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02806">
        <property type="entry name" value="Alpha-amylase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02922">
        <property type="entry name" value="CBM_48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000463">
        <property type="entry name" value="GlgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="SSF81296"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01515">
        <property type="entry name" value="branching_enzym"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword id="KW-0321">Glycogen metabolism</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000260670" description="1,4-alpha-glucan branching enzyme GlgB">
        <location>
            <begin position="1"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="398"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00685"/>
        </source>
    </evidence>
    <sequence length="716" mass="81516" checksum="A7E754D1FF133126" modified="2005-10-11" version="1">MTELSSEARAIVEGRHSDPFHYLGCHRETDRKVVRVYLPDASEVRLIDETGEEIILTRIHDAGLFLGDLPDRAERYRLRARYGADLVELEDPYRFPPILDDFDLYLLGEGTHQRIYDKLGAHPMAMEGVDGAGFVVLAPNARAVAVVGDFNFWDRRRHPMRVRGNGYWELFIPGARAGDRYKFAITAQDGSLLPLKSDPMAFAAEVRPNTASIIFDQARLPRPTPLAATINARGVPISIYEVHLGSWRRSDGNRWLSYRELAETLPDYVRELGFTHIELMPVNEHPFDGSWGYQPTGLYAPTSRFGSPEDFAHLIDACHREGLGLLLDWVPGHFPDDPHGLGRFDGTSLYEHANPLQGRHLDWDTLIYNYGRTEVVNFLVANALFWLDRYGIDGLRVDAVASMLYLDYSRPEGGWIPNKHGGRENLEAIEFLRRFNREVFGRFPNATTVAEESTAWPQVSRPIEFGGLGFGYKWNMGWMHDTLSYFEKDPIHRKHHHDQILFGLHYAFSENFILPLSHDEVVHGKRSILGRMPGDDWQRFANLRAYYAFMFGHPGKKLMFMGAEFGQEREWNHDHALDWHLLDQARHQGVQAVVRDLNKLYREVPALHEFDCDPAGFEWIVANDADHSVFAWMRKGASGRARCLVIANLTPTIHRDYRVRVPFTGRWREALNTDSAHYGGSNVGNDGAIETSGEIIPELVLTLPPLGVIFLVPEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STE0</accession>
    <name>Q3STE0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">LipA a lipoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1189</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1189"/>
    <dbReference type="EnsemblBacteria" id="ABA04451">
        <property type="protein sequence ID" value="ABA04451"/>
        <property type="gene ID" value="Nwi_1189"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1189"/>
    <dbReference type="eggNOG" id="COG4520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118535_1_0_5"/>
    <dbReference type="OMA" id="NCRQYTH"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032635">
        <property type="entry name" value="Anti_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016364">
        <property type="entry name" value="Surface_antigen_Rickettsia"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16998">
        <property type="entry name" value="17kDa_Anti_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002721">
        <property type="entry name" value="Surface_antigen_Rickettsia"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="17kDa_Anti_2" evidence="1">
        <location>
            <begin position="53"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="142" mass="14392" checksum="946A626BB2C66C25" modified="2005-10-11" version="1">MLLIAVAATLALGGCDTTGSGGPDAPGLTAFAGFWEGATGPATAAALGGLIGPKLGSALDDNDRRLAYQAQIDALERGAPGAPITWRNPDSGRHGNIVAGPEYEVEGATCRGYSHTVTIAGQLQTVRRTACRNADGRWTATG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STF4</accession>
    <name>Q3STF4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Gene transfer agent (GTA) like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1175</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314466.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1175"/>
    <dbReference type="EnsemblBacteria" id="ABA04437">
        <property type="protein sequence ID" value="ABA04437"/>
        <property type="gene ID" value="Nwi_1175"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1175"/>
    <dbReference type="eggNOG" id="ENOG5032SG3">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_164764_0_0_5"/>
    <dbReference type="OMA" id="NKHRGEI"/>
    <dbReference type="OrthoDB" id="2062495at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021791">
        <property type="entry name" value="Phage_TAC_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11836">
        <property type="entry name" value="Phage_TAC_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="106" mass="10775" checksum="709B2498717B9E6A" modified="2005-10-11" version="1">MPNKYRGEIGAELGGRHRTLVLTLGALAELESAFGAGDLMALAERFGSGRVSSRDLIRIIGAGLRGAGEAVSDDDVAVMTVEGGAAGFVRIAAGLISATFDESVAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STN4</accession>
    <name>Q3STN4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transposase, IS4 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0310</name>
        <name evidence="4" type="ordered locus">Nwi_1095</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="4">Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0310"/>
    <dbReference type="EnsemblBacteria" id="ABA03577">
        <property type="protein sequence ID" value="ABA03577"/>
        <property type="gene ID" value="Nwi_0310"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04357">
        <property type="protein sequence ID" value="ABA04357"/>
        <property type="gene ID" value="Nwi_1095"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0310"/>
    <dbReference type="KEGG" id="nwi:Nwi_1095"/>
    <dbReference type="eggNOG" id="COG3293">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055261_9_1_5"/>
    <dbReference type="OMA" id="QHNGARP"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE_Tnp_1" evidence="2">
        <location>
            <begin position="54"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="210" mass="23841" checksum="D18125D92E9F6B20" modified="2005-10-11" version="1">MGGHSSALRPAHHLREPLQPVAQGRRVGSHSVGCFKGLRWRHPNDRFVVDPRASTCGQRSKKQTRSRCMGRSRGGLTTKIHALVDACGLPIVLKITEGQAHDGRSAQDMIDTVERGDVLLADRAYDSNALRQTLAARGARANVKPMPNRVAALQFNRRLYRKRNLVERFFNKIKHYRAVATRYDKRDDNFLTSIKLASIRIWLRFNESMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STX1</accession>
    <name>Q3STX1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Binding-protein-dependent transport systems inner membrane component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_1008</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314308.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1008"/>
    <dbReference type="EnsemblBacteria" id="ABA04270">
        <property type="protein sequence ID" value="ABA04270"/>
        <property type="gene ID" value="Nwi_1008"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1008"/>
    <dbReference type="eggNOG" id="COG1177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_3_0_5"/>
    <dbReference type="OMA" id="FPMFVWG"/>
    <dbReference type="OrthoDB" id="1510342at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="107"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="67"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="273" mass="29252" checksum="FF91E59BD67346C3" modified="2005-10-11" version="1">MMARPALARLSPFNITSLALGLAFLYLPIVILVIYSFNASRLVTVWGGWSLRWYVEFFNDEAMLSALAMSLRVAAMSATAATILGTLAAVALTRAGSFHGRTLFSGMLYAPLVMPEVITGLSLLLLFVAVDAQRGFWTVTIAHTTLTMCFVTVVVQSRLGSLDRSLEEAAMDLGCDPFWAFVKVTLPLILPSVVAGWMLAFTLSMDDVVIASFTTGPGSTTLPIRIYSEVRLGVKPEINAICTLIVGSIAVVIVVASLVSKLSSRQGENAAPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU16</accession>
    <name>Q3SU16_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Membrane fusion protein (MFP) family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_0963</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell inner membrane</location>
            <topology evidence="8">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314266.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0963"/>
    <dbReference type="EnsemblBacteria" id="ABA04225">
        <property type="protein sequence ID" value="ABA04225"/>
        <property type="gene ID" value="Nwi_0963"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0963"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023976_1_1_5"/>
    <dbReference type="OMA" id="FPIVPGM"/>
    <dbReference type="OrthoDB" id="1395473at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010129">
        <property type="entry name" value="T1SS_HlyD"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01843">
        <property type="entry name" value="type_I_hlyD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="159"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA04225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="447" mass="49170" checksum="E7CCF896569926C8" modified="2005-10-11" version="1">MSMTATGDQPWHVDLPRSTRGLTLAGVVIVAVTVLGFGVWGNTAPIAGAVVASGVFVATGQNKIIQHLEGGMIDEIKVREGDTVEPGQILVELDRTSAKAEMQRLFLRNARLTALDARLQAQVREETEVHFPPELASDSKDPQIRSIVNAQLLSFTAQRNNANSDIAGIEDSIKALNERIAGSKVQLDGVRRQIALIEEDIKTKEHLLAAGLVRKPEVLLLQRNQANLEGEVGRIMGDIGDAKERISRAVEQINGVRKVAIKTAVEQMHEVRGELADVRERMQSAKGVLDRTTIRAPVKGVVVKLRFHTRGGVVEPGKPIMELLPLDEPLIIEARVRPQDIDSVKHGQEAMVRLTALSQRITPMVSGDVIYLSADTVADEKKSQQTGPSDIYVIRVRLNNAEVVAIHGFHPTPGMPAEVFIKTSERTFFQYIMKPIDDSMSRAFRER</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU26</accession>
    <name>Q3SU26_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CRISPR-associated endoribonuclease Cas2</fullName>
            <ecNumber evidence="9">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cas2</name>
        <name evidence="10" type="ordered locus">Nwi_0953</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Functions as a ssRNA-specific endoribonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer, forms a heterotetramer with a Cas1 homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the CRISPR-associated endoribonuclease Cas2 protein family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="9"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314256.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0953"/>
    <dbReference type="EnsemblBacteria" id="ABA04215">
        <property type="protein sequence ID" value="ABA04215"/>
        <property type="gene ID" value="Nwi_0953"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0953"/>
    <dbReference type="eggNOG" id="COG3512">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150500_0_0_5"/>
    <dbReference type="OMA" id="TDKQFGM"/>
    <dbReference type="OrthoDB" id="1841250at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01471">
        <property type="entry name" value="Cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021127">
        <property type="entry name" value="CRISPR_associated_Cas2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019199">
        <property type="entry name" value="Virulence_VapD/CRISPR_Cas2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09827">
        <property type="entry name" value="CRISPR_Cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01573">
        <property type="entry name" value="cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="5 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA04215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="101" mass="11838" checksum="6E8165CAEF10E0C6" modified="2005-10-11" version="1">MWLVVLFDLPVGTKKERKAASGFRLKLLDLGFEMSQFSVYLRFCAGKEQAAAYERKIEQFMPASGKIHIMAITDKQYENIRTYRGRKRERSPENPNQFVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU78</accession>
    <name>Q3SU78_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0901</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04163">
        <property type="protein sequence ID" value="ABA04163"/>
        <property type="gene ID" value="Nwi_0901"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0901"/>
    <dbReference type="HOGENOM" id="CLU_2451638_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="89" mass="9255" checksum="99272B34807B3FFC" modified="2005-10-11" version="1">MTVRAADQDPGSGGGHTKFPSHGCDAFFAEMVHLEGATCPFRQFPQAAAEIASRCRSNTIVSGDGMSSGIASNSASVRATCGAHRSRRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU96</accession>
    <name>Q3SU96_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, GntR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0881</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0881"/>
    <dbReference type="EnsemblBacteria" id="ABA04145">
        <property type="protein sequence ID" value="ABA04145"/>
        <property type="gene ID" value="Nwi_0881"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0881"/>
    <dbReference type="eggNOG" id="COG1167">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_0_1_5"/>
    <dbReference type="OMA" id="RIGFMVM"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="2">
        <location>
            <begin position="17"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="478" mass="51804" checksum="B6131034F64CC5B0" modified="2005-10-11" version="1">MGSRHDLKLELTGDGERPVFMNIAYSIIREIERGRLKPGDPLPGTRALSTSLKVHRNTVDAAYQELTMQGWLVARPSRGTFVANDLPEPAGGETPPPTLRAPLDPAGGTARPMLRVSDGAPDARLMPATQLARAFRTALTSPAFVGGGDYSDPRGAIRLRTALSSYLISERGLTASADDMMITRGSQMGLYLAAAALVEPGEAIAVEEPGYPLAWSAFRAAGARVVGIPVDAHGIDVDRLAAIADREPELRAIYVTPHHQYPTTVTLGASRRLKLLDLARRKRLVLIEDDYDHEYRFEGRPVLPLAARVEAEGVVYLGSLSKLLAPAVRVGYAIAAPAVLTRMADRREAIDRQGDVPLEQALASLIEDGELRRHTRKARRIYGEKRDLFAAAIRRELGQHVAFDLPAGGLALWLRLRAGLDAETWAANAAQVGLAVSSGVKFVLDAAHAPHAFRLGYASLTEIELSRVATLLKRTIDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU99</accession>
    <name>Q3SU99_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0878</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314183.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0878"/>
    <dbReference type="EnsemblBacteria" id="ABA04142">
        <property type="protein sequence ID" value="ABA04142"/>
        <property type="gene ID" value="Nwi_0878"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0878"/>
    <dbReference type="eggNOG" id="COG1225">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076204_1_0_5"/>
    <dbReference type="OMA" id="HCPYVKH"/>
    <dbReference type="OrthoDB" id="1617952at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016209">
        <property type="term" value="F:antioxidant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="1">
        <location>
            <begin position="8"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="184" mass="20211" checksum="16BF1D7DD3BEC2AE" modified="2005-10-11" version="1">MATTVTQVTFDTPAPEFRLPATDGKTYGLDDLAGVNGTVVVFICNHCPYVKAVIDRMVADARVLMSEDIGFVAICSNDAASHPEDSFENMKKFAETHGFPFPYLHDETQEVARAYGAVCTPDFFGYSADRKLKYRGRLDEGRKTPPRAEAPRELVEAMRAIARTGVAPADQNPSLGCSIKWKDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUD9</accession>
    <name>Q3SUD9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Integrase, catalytic region</fullName>
            <ecNumber evidence="2">2.7.7.49</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0838</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04102">
        <property type="protein sequence ID" value="ABA04102"/>
        <property type="gene ID" value="Nwi_0838"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0838"/>
    <dbReference type="eggNOG" id="COG2801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027402_15_1_5"/>
    <dbReference type="OMA" id="HIDITET"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="79"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="286" mass="32795" checksum="EC09764669FA8AE2" modified="2005-10-11" version="1">MADLPTGPKDPRSTVLSSEEEAIIVAFRKHTLLPLDDCLYALQPTIPHLSRSSLHRCLHRHGISRLPEVEGDKPIRKTFRSYPIGYFHIDIAEVQTAEGKLRLFVAIDRTSKFAYAELHQEAGKMVAAQFLRNLIVAVPYAIHTVLTDNGIQFTNHARHKYAFHHIFDRVCDENGIEHRLTRINHPWTNGQVERMNRTIKDATVKRFHYDNHDQLRRHLQDFIKAYNFGRRLKTLKAPHTLRVHLQTMDFRARSIHHRSNPSNAGTEHHAAFGAADGLALSPPFAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUF6</accession>
    <name>Q3SUF6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Plasmid and phage replicative helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0820</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314133.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0820"/>
    <dbReference type="EnsemblBacteria" id="ABA04085">
        <property type="protein sequence ID" value="ABA04085"/>
        <property type="gene ID" value="Nwi_0820"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0820"/>
    <dbReference type="eggNOG" id="COG3598">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_373776_0_0_5"/>
    <dbReference type="OrthoDB" id="1529872at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="743" mass="80008" checksum="EC44029CA1CCFD9A" modified="2005-10-11" version="1">MTTHAPESEPDRTAMLRHVELVFGGGFDGALDGLVELAWTDPATGSLRNAQMFGTDQLEELVERAAELNRTERCNVYVGAALRKPGTALAKRTADSDFHSAPFAWADIDDDCVEAAIKAAKAAGVPATMTVVTGRHPHMRAQFWWRLVDAERDAAAIKALCSSIGLALGGDSTVSNPGRVLRLGGSIAWPLKPGRVVESTEVHIPEDDRPPAYWASALTQAFAAPAPLLQTAPAASEPSAPSMAPSTPKPLELPIGSLSVEATLAAIQRNDHWHQNTVRLVGNWVARGLSNAEILAFAPALTIGNGPDGRSYTVDQTCLQLNSMITGARRKWNLPNPVVTIEDKLPPPPMEIEWEDGASAAMIPRRRWLVGSFAIRGHLTVLVAPPGAGKSTLGIALAVAGVTGRGEIVGETVHETIKAWVWNNEDDRNELRRRLAAIMQQWNVAPADLRGKLGLNSGSERPLVIAKATKDGAVVRLPDIEAIIERVQAEGIGLLIVDPFVETHEVDENNNAQIKAVAAMWREVARRGDCAVVIVHHTGKPPSASPDAWTGSLSASRGASSLGGVARIMRTLFAMSQSDADKFGLDADERRLWVRLDDAKANLSLASGSARWFKRVSIIIANGEEVGALVPGDPNERAPQVDRAAEIESALFEAIRTAWNAGSPLSEQPRAKDRYAPGIVGKAMRIASETVADVLARLMGGGAIERTRFDAKTKTYGLRIVPLDERDMRSDQRRNHEPAEEFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUI7</accession>
    <name>Q3SUI7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0789</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314102.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0789"/>
    <dbReference type="EnsemblBacteria" id="ABA04054">
        <property type="protein sequence ID" value="ABA04054"/>
        <property type="gene ID" value="Nwi_0789"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0789"/>
    <dbReference type="eggNOG" id="COG0791">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100188_0_0_5"/>
    <dbReference type="OMA" id="YLGAHEK"/>
    <dbReference type="OrthoDB" id="682655at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013423">
        <property type="entry name" value="CHP02594"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02594">
        <property type="entry name" value="TIGR02594"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="189" mass="20490" checksum="76F9D038AEA16D93" modified="2005-10-11" version="1">MLPAQYRWLEAEPGPRMIVEALKEYGTLEAPGAADNPKIIGWQTELEAAGLGRVYAGVYRHDAIPWCGLFMAIIAHRANAERRAERNPPRLYLAALQWASFGKTVPKGGGALGDVLVFKRKGGGHVGLYVGHDASAFHVLGGNQSDRVSITRLSRNRLVAVRRPAYRVQPANVRAVPLAQSGSLSVNEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUJ1</accession>
    <name>Q3SUJ1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0785</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0785"/>
    <dbReference type="EnsemblBacteria" id="ABA04050">
        <property type="protein sequence ID" value="ABA04050"/>
        <property type="gene ID" value="Nwi_0785"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0785"/>
    <dbReference type="eggNOG" id="ENOG502ZMIR">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1018748_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="273" mass="30285" checksum="743C0C3266560E24" modified="2005-10-11" version="1">MTQSRFRWVTVQRALDLLVAEFTNARAISFIEEIGCGPEVDRLSSAERTTPKLRNLISRACREEPQRMDTEGSPLTDRVVREAASYVPAPESVTPWNNEPPTFSTPVAAFLNSLAVDGWGVEQRQLMPHTAVPIVEPRSRLRQVLQDSSATEALRRLDQLEKGLDEGHWESANSDVRGFLNAVFDTIAERHPQTRDQGLKEGAARARLQDVGFFKPDARDSKKSYEGKFVQALAELLGSDGAHTGASDGDSAVFRYAIAIVTADYFVARARKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUN0</accession>
    <name>Q3SUN0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HNH endonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0745</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0745"/>
    <dbReference type="EnsemblBacteria" id="ABA04011">
        <property type="protein sequence ID" value="ABA04011"/>
        <property type="gene ID" value="Nwi_0745"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0745"/>
    <dbReference type="eggNOG" id="COG1403">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099824_1_0_5"/>
    <dbReference type="OMA" id="WRIIGTG"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00085">
        <property type="entry name" value="HNHc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029471">
        <property type="entry name" value="HNH_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003615">
        <property type="entry name" value="HNH_nuc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14279">
        <property type="entry name" value="HNH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00507">
        <property type="entry name" value="HNHc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HNHc" evidence="1">
        <location>
            <begin position="87"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="191" mass="22220" checksum="C6138D93D6AA515D" modified="2005-10-11" version="1">MLWRRYLNAHVSQGGWPVLVLNADFRPLSYYPLSLWSWQDAIKAVFLDRVNIVERYDRAVRSPSFEIQLPSVVSLKSFVKPSTHPAFTRFNVFLRDRFMCQYCTAVEDLTFDHIIPRSKGGQTTWDNVVAACSPCNLRKGSLTPQQAGMFPRQAPFAPTVHQLHRNGRLFPPNYLHDSWLDYLYWDTELDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUV5</accession>
    <name>Q3SUV5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cytochrome c553</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_0670</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="PTM">
        <text evidence="4">Binds 2 heme c groups covalently per subunit.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0670"/>
    <dbReference type="EnsemblBacteria" id="ABA03936">
        <property type="protein sequence ID" value="ABA03936"/>
        <property type="gene ID" value="Nwi_0670"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0670"/>
    <dbReference type="eggNOG" id="COG2863">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076280_2_1_5"/>
    <dbReference type="OMA" id="NVSAWFA"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024167">
        <property type="entry name" value="Cytochrome_c4-like"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000005">
        <property type="entry name" value="Cytochrome_c4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4 6" id="KW-0349">Heme</keyword>
    <keyword evidence="3 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229331" evidence="7">
        <location>
            <begin position="25"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="8">
        <location>
            <begin position="24"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="8">
        <location>
            <begin position="115"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="binding site" description="covalent" evidence="4">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="4">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="covalent" evidence="4">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="4">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
            <label>2</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
            <label>2</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000005-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000005-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA03936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="205" mass="22141" checksum="BA68FB4225AEF854" modified="2005-10-11" version="1">MSRFLIVGMTSLAAFALLVRHAFADAIVDAPDRPWEACGNCHGVDGISATAHFPKLAGQKRAYIEKQLRDFRSGLRINDNGQMSGTGDFPDHALELAAAYFSQLSAPSPESRENNNISRAKTIVESGVPKAGVPACRSCHNGDANLEPWLEAQHAGYLAKQLKDFKSGVRGNDKVMADIARRLSDDDIEEVAGFLASTPRPPGKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUZ9</accession>
    <name>Q3SUZ9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0625</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03892">
        <property type="protein sequence ID" value="ABA03892"/>
        <property type="gene ID" value="Nwi_0625"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0625"/>
    <dbReference type="HOGENOM" id="CLU_188387_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="68" mass="7912" checksum="3213E7DE444E8C04" modified="2005-10-11" version="1">MLIHLERYVAVEKLIDDAWEMSQQLDLGLVKLLLEMAKLELYEEMQAVIGSNEIKRAEKRAGGRRQLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV38</accession>
    <name>Q3SV38_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methionine biosynthesis MetW</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0586</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313914.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0586"/>
    <dbReference type="EnsemblBacteria" id="ABA03853">
        <property type="protein sequence ID" value="ABA03853"/>
        <property type="gene ID" value="Nwi_0586"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0586"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091323_0_0_5"/>
    <dbReference type="OMA" id="LPYEWYN"/>
    <dbReference type="OrthoDB" id="957193at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010743">
        <property type="entry name" value="Methionine_synth_MetW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07021">
        <property type="entry name" value="MetW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02081">
        <property type="entry name" value="metW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="229" mass="25855" checksum="98B00773B6A42902" modified="2005-10-11" version="1">MSAEQPSLPLDEVASRPVDRYRSDHLLLAEMIEPGSKVLDVGCGDGDLLQLLETRGISGRGIELSREGVNRCVAKGLAVVQGDADTDLVNYPDDAFDYVILSQTLQATRQPKMVLENLLRIGRRAVVSFPNFGYWKMRVQLLVGGHMPRTDNLPATWYDTPNIHFCTIKDFVQLCDEINVKMERAVALNLYGRPLRFNAPWWFWNMFGEQGVFLLSRAEKIARTPGQDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV43</accession>
    <name>Q3SV43_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0581</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0581"/>
    <dbReference type="EnsemblBacteria" id="ABA03848">
        <property type="protein sequence ID" value="ABA03848"/>
        <property type="gene ID" value="Nwi_0581"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0581"/>
    <dbReference type="eggNOG" id="COG4093">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064708_0_0_5"/>
    <dbReference type="OMA" id="PFRLEVR"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018666">
        <property type="entry name" value="DUF2125"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09898">
        <property type="entry name" value="DUF2125"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="414" mass="44670" checksum="370F95113FE9F529" modified="2005-10-11" version="1">MVEDPADPAHQEPAFRMNAANNASSKYRLWPIFVMPVLVVIAAAAWSAFWFYAASQVDQTVDAWRAREAASGRIYDCAKRTVAGFPFRLEVRCEDATVELRSQTAEQAAAQMPPLTARLGDILVIAQIYTPRLLIAEFKAPATLSRHGQPVMVVDWRVGRSSIVGLPDTPKSADIVFDDLAIDRMVESVRTPAARAAHVELHGRTAEKSTLDKPMIETAVELSGATLSDIHPVLTQPFNAEVGAIVHGLKDFTPKPWPVRFRELQAAGGRIEIRQSRIQQGDLIAIAAGSLGLTADGHLDGELHMTATGFEKMIPALGIDKMLEQGVPQETLDKVAPGVKSKDIDNLFGALDRAIPGLGRAVRENANVGVAAGLAAIGQEATLENKKARSFPLRFVAGAVFIGPLKVAQTPPLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV50</accession>
    <name>Q3SV50_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Response regulator receiver</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0574</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313902.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0574"/>
    <dbReference type="EnsemblBacteria" id="ABA03841">
        <property type="protein sequence ID" value="ABA03841"/>
        <property type="gene ID" value="Nwi_0574"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0574"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_8_5"/>
    <dbReference type="OMA" id="MDGHETV"/>
    <dbReference type="OrthoDB" id="1630092at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="3"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="130" mass="13516" checksum="33285C4F260287D2" modified="2005-10-11" version="1">MARVLIADDEDSMRSLVARAIAMDGHEIVTAADGAEALDILSADAHTFDLLLTDIKMPIMDGIALALAAARDFPGLIILLMTGFADQRERASGLNAIVHDVVTKPFSVADIRAAVAGALTTQTGGTISSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV65</accession>
    <name>Q3SV65_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">HWE histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0559</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313887.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0559"/>
    <dbReference type="EnsemblBacteria" id="ABA03826">
        <property type="protein sequence ID" value="ABA03826"/>
        <property type="gene ID" value="Nwi_0559"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0559"/>
    <dbReference type="eggNOG" id="COG3920">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073829_0_0_5"/>
    <dbReference type="OMA" id="PYGHVRF"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004673">
        <property type="term" value="F:protein histidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011102">
        <property type="entry name" value="Sig_transdc_His_kinase_HWE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07536">
        <property type="entry name" value="HWE_HK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00911">
        <property type="entry name" value="HWE_HK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="HWE_HK" evidence="2">
        <location>
            <begin position="147"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="337" mass="36233" checksum="C21AC41C797E1CD6" modified="2005-10-11" version="1">MAKRTEARWIEEFRRGWRGVSEPPAALGFGLAAVCLMLATAFRFGISLLHADVPYGAYLPGVFAAAAFGGERAGIAAAIGGAALGLMLNFGEAPQAVAAAILMAVYFTVAALIILGVRRHRSLVVHHRELTTELEKEQHYRELVVEELRHRLKNKLATVHAVTKQALHNYPEARTKIDGCINALATTDDLIARTDDRGCDILELLRSELGPYGHVRFTLNGDSLFLPAKLAVSLALMFHELATNAAKYGAFSTPHGLLNVSWTVTNGTLSIVWDESGGPMITAQGKPGFGTQLLNAGLRGFGGKAEIAFLGTGLHCIMQCRIDPPPADKKAETLSEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV76</accession>
    <name>Q3SV76_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Glycosyl transferase, family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0548</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313876.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0548"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03815">
        <property type="protein sequence ID" value="ABA03815"/>
        <property type="gene ID" value="Nwi_0548"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0548"/>
    <dbReference type="eggNOG" id="COG1216">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005003_3_0_5"/>
    <dbReference type="OMA" id="GGWSWDP"/>
    <dbReference type="OrthoDB" id="957193at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="784" mass="85607" checksum="A8B5550494C3C52F" modified="2005-10-11" version="1">MTHVIRSRKAEIAQPRLVSRLALGADGTVSGFVFDPEAPERRFTIDILLDGLVLKTVHADAFAPECAEPDRSNTCGFAVSVDTDLLDAARHLSARLANLETPVGQSIDLENDTTSRSDLRPTCKLRWLGGLHFHGWIDSEAIVTLEAIVDGESVAQVRATAWTHIGGDAEGGVSRNVRAFDFHVPQRLADGRIHRILLRKEDGDRLPAAATFVAFPDGLAGMIDVIGGYAAERLRGELYDRLIPASLPLHDYAGWRERFPLPEPQPSSLRLAVVIAGSSGTQQTIASLEAQSHENWTAGAIDGEPLSIDGDALLDFLEDAAPDTSHIVVTMAGVSLERTALARIAAAFDSHPDALAIYGDLDFLADDGRIWPLAFPAFDYERLLEQGYCVHLFAVRREALLAAIEAARPDSLYRLFNSLLDQIGPRQADILHLPGALATMPRLDRTTAGALLSAASQSHLRARGIHASVTRETGNFFPAVRIKRPVSRQRVTVIIPTRDRVSLLQRCLETILPAVERCHADILVVDNDSAHPQAIDFLSDLPRRGIRTLRVEGPFNFARLNNEAAATLDSDILCLLNNDIEASSDDWLEEMLTRLGEPDVGAVGALLTWPGGVVQHGGVVLGINFSVAHAFTDRLSGDPGFLDQLLVAHECSAVTAACLATRRSDYLAVGGMDEARFAVALNDVDYCLRLREAGKRIVLTPHAKLVHAESASRGSDNRADRRGRFEHELNLLRARWGEFLNEDPAYNPQLSRDGVPYRGLAWPPGRRAPRYNHPPVAHDLPLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVC4</accession>
    <name>Q3SVC4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Diguanylate cyclase/phosphodiesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0500</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0500"/>
    <dbReference type="EnsemblBacteria" id="ABA03767">
        <property type="protein sequence ID" value="ABA03767"/>
        <property type="gene ID" value="Nwi_0500"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0500"/>
    <dbReference type="eggNOG" id="COG2199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2200">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_70_50_5"/>
    <dbReference type="OMA" id="ERRYMQD"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGDEF" evidence="3">
        <location>
            <begin position="197"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="EAL" evidence="2">
        <location>
            <begin position="341"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="592" mass="64926" checksum="7401680FB2619F32" modified="2005-10-11" version="1">MTSAISPAVNPSSGADASQTISAPAGLPQATQEPRVSAILTALGRAAFVWDIPSDAINWTEGAASIFKGIDPFLLATGARYGRLIEPSGSVRTNIFSAAPTTRRRFDEGVPYFIEYGVRASASAPMLWIEEAGCWFADADGKVVRVEGIVRLENERRARDEQLINLSRNDALTGELNRTYLTAALAEAIEEAARYRSPVAFMMVGIDHLARINNAFGFDVADEVISDVARRIRRRLRSGDTLGRFSGNRFGVILKNCGLDEVSIAAERLLAGIREEIVMTKSGPVSVTASIGAVLAPRYAQTADEAINRAQEILEVAKGRRCGSLAVWRPDTEREAQRRINIRVTDEIVTALNERRILTAFERVVDARTREAAFYECLVRMQQQNGQILLAPDVVPVAEKLGLIRLVDHRVLELVIAELAEAPHVQLSLNISPETTMDVDWWAAIEALMGAHPGVAERLIVEITETAAIQDIDDVQRFVTRLKNLGSRIAIDDFGAGYTSFRNLRKLGVDIVKIDGAFVQNLARSSDDRAFVQTLIDLARRLDIRTVAEWVQDEQSAAMLRDWGCDFIQGRLIGLASPSRPWSGPAAVPATN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVE6</accession>
    <name>Q3SVE6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0478</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the YggT family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313809.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0478"/>
    <dbReference type="EnsemblBacteria" id="ABA03745">
        <property type="protein sequence ID" value="ABA03745"/>
        <property type="gene ID" value="Nwi_0478"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0478"/>
    <dbReference type="eggNOG" id="COG0762">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136788_0_1_5"/>
    <dbReference type="OMA" id="WLINFQV"/>
    <dbReference type="OrthoDB" id="2069856at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003425">
        <property type="entry name" value="CCB3/YggT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33219">
        <property type="entry name" value="PTHR33219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02325">
        <property type="entry name" value="YGGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="96" mass="10967" checksum="B411863CD9A55C0E" modified="2005-10-11" version="1">MRAILDIVLIILDLYVWLLIASAILSWLIAFNVVNTRNQFVGAVAEFLERITEPLLAPIRRLLPNLGGLDISPIILILIILFMQRVITYYIYPAVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVH1</accession>
    <name>Q3SVH1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nuclease (SNase-like protein)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0453</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313784.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0453"/>
    <dbReference type="EnsemblBacteria" id="ABA03720">
        <property type="protein sequence ID" value="ABA03720"/>
        <property type="gene ID" value="Nwi_0453"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0453"/>
    <dbReference type="eggNOG" id="COG1525">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064698_0_0_5"/>
    <dbReference type="OMA" id="RGWVLDR"/>
    <dbReference type="OrthoDB" id="1719840at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016071">
        <property type="entry name" value="Staphylococal_nuclease_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00565">
        <property type="entry name" value="SNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00318">
        <property type="entry name" value="SNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50199">
        <property type="entry name" value="SSF50199"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229344" evidence="1">
        <location>
            <begin position="30"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="TNase-like" evidence="2">
        <location>
            <begin position="36"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="259" mass="28282" checksum="6838A2C490AF4BC2" modified="2005-10-11" version="1">MTAVPRCFCNLLFAVPLSAISFATVPALADGCQLDIQGEGVVSAVIDARTLRLEDGRDVRLAGVEVVALKSDDRRSNLAGLIGQHVILRGEHDDPDRYGRQTGLLFLDHTARSVQSELLARGEALASADVTNKACAAELAAAETVARQGRLGIWAESSVIKSAERPDEILTRIGRFTIVEGHVRSIRQAGGTLYLNFGPRWTRDFAATISKRIMPSFKAAGVAPRSFEHRRVRVRGWVERRGGPRIEVFRVGQIDAEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW24</accession>
    <name>Q3SW24_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0249</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + O-phospho-L-tyrosyl-[protein] = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:10137"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:82620"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0249"/>
    <dbReference type="EnsemblBacteria" id="ABA03517">
        <property type="protein sequence ID" value="ABA03517"/>
        <property type="gene ID" value="Nwi_0249"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0249"/>
    <dbReference type="eggNOG" id="COG0394">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071415_3_1_5"/>
    <dbReference type="OMA" id="LFCCDHN"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023485">
        <property type="entry name" value="Ptyr_pPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036196">
        <property type="entry name" value="Ptyr_pPase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01451">
        <property type="entry name" value="LMWPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00226">
        <property type="entry name" value="LMWPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52788">
        <property type="entry name" value="SSF52788"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LMWPc" evidence="4">
        <location>
            <begin position="26"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="170" mass="19140" checksum="34CC0F28B8763F86" modified="2005-10-11" version="1">MAARRARAKRKRLEPMAAPARVHSPQSVLFACAHNSVRSPMAASLLQQMFPRTLYVRSAGVRTGELDPFAVAVMAELGQDISKHRPMTFEEIEDWEGLNFDLIITLSPEAHHKALELTRTIAADVEYWPTPDPTIIEGNREQRLQAYRDVCDGLLSRIRKRFARGGGFKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW66</accession>
    <name>Q3SW66_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase FkbM</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0207</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313543.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0207"/>
    <dbReference type="EnsemblBacteria" id="ABA03475">
        <property type="protein sequence ID" value="ABA03475"/>
        <property type="gene ID" value="Nwi_0207"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0207"/>
    <dbReference type="eggNOG" id="COG2242">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082597_0_0_5"/>
    <dbReference type="OMA" id="GWYGPWT"/>
    <dbReference type="OrthoDB" id="1340956at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006342">
        <property type="entry name" value="FkbM_mtfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05050">
        <property type="entry name" value="Methyltransf_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01444">
        <property type="entry name" value="fkbM_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_21" evidence="1">
        <location>
            <begin position="36"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="235" mass="26095" checksum="6E16875CC1B0AFAE" modified="2005-10-11" version="1">MNARFVWRALKARWRDQRAELSVIGGHVRPGDTVCDVGAHKGSYVYWLSRWVGNSGRVLAFEPQPSLAAYLREVAPAHNVTVEQKALWSGSGVLDLAIPAIDSPGASLVAPVAGGTRMQVPVVALDDYLRPGQRVSLIKVDAEGAELAIFKGARRTLSEARPWLIFECETRHLAETNVEDVFGLLDRLGYEGFFFRQGSLHPVSAFDPAIHQKQSGPRFWDHPDYCNNFLFKPRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW87</accession>
    <name>Q3SW87_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metallophosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0186</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0186"/>
    <dbReference type="EnsemblBacteria" id="ABA03454">
        <property type="protein sequence ID" value="ABA03454"/>
        <property type="gene ID" value="Nwi_0186"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0186"/>
    <dbReference type="eggNOG" id="COG1407">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075478_2_0_5"/>
    <dbReference type="OMA" id="WINGNHD"/>
    <dbReference type="OrthoDB" id="1690667at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026336">
        <property type="entry name" value="P_estr_lig_assc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024173">
        <property type="entry name" value="Pesterase_MJ0037"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000887">
        <property type="entry name" value="Pesterase_MJ0037"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04123">
        <property type="entry name" value="P_estr_lig_assc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="1">
        <location>
            <begin position="46"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="241" mass="26179" checksum="EDF16E44FEC6DC67" modified="2005-10-11" version="1">MPDLNRCARDALPPWEEQMSRVAAVTVAGADFAADLSGALFWERERLLVVSDLHLEKGSSFAMRGVLLPPYDTVATLDRLAAVIRRFDPRTVIALGDSFHDRDAHNRLCARDREALSGLQAGRRWIWISGNHDPAPPSGLGGLVATEWEIGGIVFRHEPTGAFGEIAGHLHPKARVSARGRSMERRCFASDGARMVMPAFGAFAGGLSIRNGAFARIFSADFTAHVLGHSRLHAIAASRCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWD0</accession>
    <name>Q3SWD0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0143</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0143"/>
    <dbReference type="EnsemblBacteria" id="ABA03411">
        <property type="protein sequence ID" value="ABA03411"/>
        <property type="gene ID" value="Nwi_0143"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0143"/>
    <dbReference type="eggNOG" id="ENOG5033B2H">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109713_0_0_5"/>
    <dbReference type="OMA" id="MIFLRGR"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="158" mass="17101" checksum="89F4B571FABE7CF3" modified="2005-10-11" version="1">MISGQMTRIVTGLLAAVFVLGHVAASEAVVRIGEDRGGRIGTYIDKFEAVRSSGQSVIIDGLCASACTIVLGTVPHDRICVTSHAALGFHAAWDLGSNGRTVINSEATHMLYSMYPSAVQRWIKQRGGLTKRMIFLRGRQLADMYRPCYLDAQASSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWG0</accession>
    <name>Q3SWG0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase-like protein</fullName>
            <ecNumber evidence="2">3.1.2.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0113</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.2.6" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313450.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0113"/>
    <dbReference type="EnsemblBacteria" id="ABA03381">
        <property type="protein sequence ID" value="ABA03381"/>
        <property type="gene ID" value="Nwi_0113"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0113"/>
    <dbReference type="eggNOG" id="COG0491">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_5_0_5"/>
    <dbReference type="OMA" id="GAWGTNC"/>
    <dbReference type="OrthoDB" id="1581636at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004416">
        <property type="term" value="F:hydroxyacylglutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="22"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="224" mass="24021" checksum="9AABDF652B957C37" modified="2005-10-11" version="1">MTREDQTRAKVGAAIIPVTPFRQNCTLLWCEATRDGVVIDPGGDIPLIRAAIKQAGVTVGRIWLTHGHIDHVGGAAELRDALKVPIMGPHVADKFLLDHVVESGRHYGMTGMRDFVPDRWLEDGDQVEVGQLAFDVLHCPGHSPGSVVFFNADLHFAHVGDVLFNGSVGRTDLPGGSRAVLIKSITDKLLPLGDDVAFICGHGPGSTIGHERVSNPFLTGNVCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWN0</accession>
    <name>Q3SWN0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Coenzyme A biosynthesis bifunctional protein CoaBC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">DNA/pantothenate metabolism flavoprotein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Phosphopantothenoylcysteine synthetase/decarboxylase</fullName>
            <shortName evidence="3">PPCS-PPCDC</shortName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName evidence="3">Phosphopantothenoylcysteine decarboxylase</fullName>
                <shortName evidence="3">PPC decarboxylase</shortName>
                <shortName evidence="3">PPC-DC</shortName>
                <ecNumber evidence="3">4.1.1.36</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">CoaC</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="3">Phosphopantothenate--cysteine ligase</fullName>
                <ecNumber evidence="3">6.3.2.5</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">CoaB</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="3">Phosphopantothenoylcysteine synthetase</fullName>
                <shortName evidence="3">PPC synthetase</shortName>
                <shortName evidence="3">PPC-S</shortName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="3" type="primary">coaBC</name>
        <name evidence="7" type="ordered locus">Nwi_0043</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes two sequential steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. In the second step the latter compound is decarboxylated to form 4'-phosphopantotheine.</text>
    </comment>
    <comment type="function">
        <text evidence="4">Catalyzes two steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>(R)-4'-phosphopantothenate + CTP + L-cysteine = CMP + diphosphate + H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine</text>
            <dbReference type="Rhea" id="RHEA:19397"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:59458"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="6.3.2.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-phosphopantetheine + CO2</text>
            <dbReference type="Rhea" id="RHEA:16793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:59458"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="4.1.1.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="3">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 2/5.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">In the C-terminal section; belongs to the PPC synthetase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">In the N-terminal section; belongs to the HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.36" evidence="3"/>
    <dbReference type="EC" id="6.3.2.5" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313382.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0043"/>
    <dbReference type="EnsemblBacteria" id="ABA03311">
        <property type="protein sequence ID" value="ABA03311"/>
        <property type="gene ID" value="Nwi_0043"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0043"/>
    <dbReference type="eggNOG" id="COG0452">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033319_0_1_5"/>
    <dbReference type="OMA" id="LDMIVAN"/>
    <dbReference type="OrthoDB" id="1346419at2"/>
    <dbReference type="UniPathway" id="UPA00241">
        <property type="reaction ID" value="UER00353"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004632">
        <property type="term" value="F:phosphopantothenate--cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004633">
        <property type="term" value="F:phosphopantothenoylcysteine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015941">
        <property type="term" value="P:pantothenate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02225">
        <property type="entry name" value="CoaBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035929">
        <property type="entry name" value="CoaB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005252">
        <property type="entry name" value="CoaBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007085">
        <property type="entry name" value="DNA/pantothenate-metab_flavo_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04127">
        <property type="entry name" value="DFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102645">
        <property type="entry name" value="SSF102645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="SSF47598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="SSF52507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00521">
        <property type="entry name" value="coaBC_dfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3 4" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="3 4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 4" id="KW-0288">FMN</keyword>
    <keyword evidence="3 4 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 3 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="5">
        <location>
            <begin position="73"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="DFP" evidence="6">
        <location>
            <begin position="257"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphopantothenoylcysteine decarboxylase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphopantothenate--cysteine ligase" evidence="3">
        <location>
            <begin position="262"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="359"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="376"/>
            <end position="379"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="396"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="410"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="414"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="479" mass="50532" checksum="A834548AB9AD820B" modified="2005-10-11" version="1">MASLTVRKLDDAVKARLRARAAVSGRSVEEEIRVILREATVIPPPTAFDDPASRNAASLRDKGFFNVSSRLTKVTVIVGGGIAAYKALDLIRRLRERGFFVRCVMTNAAQQFVTPLSAGSLSNERCYTDLFDASSEFDAGHIRLARDCDLIVVAPATADLMAKMANGLANDLASAILLASDRPVLLAPAMNPLMWNNPATRRNVARLARDGVAMIGPDAGEMAEAGEAGVGRMAEPVAIAAAAARMLGPPQTQAQPLKGRRVLITAGPTREAIDPVRYIANRSSGKQGFAIAAAAQAAGAEVTLISGPVDLDDPPGVTVAHVESARQMLAAVEAALPSDIAIFTAAVADWRVAHAGAQKLKKTEAGVPPLQLIENPDILATISNLRDERPPLVIGFAAETEHLIGNAKAKFARKGCDWMIANDVSPGTGVMGGDRNTVHLLTRGEDGEIRTESFPVMTKEEVAAMLIERIISALPGPAA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-04-17" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWQ7</accession>
    <name>YBEY_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endoribonuclease YbeY</fullName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ybeY</name>
        <name type="ordered locus">Nwi_0016</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Single strand-specific metallo-endoribonuclease involved in late-stage 70S ribosome quality control and in maturation of the 3' terminus of the 16S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the endoribonuclease YbeY family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWQ7"/>
    <dbReference type="SMR" id="Q3SWQ7"/>
    <dbReference type="STRING" id="323098.Nwi_0016"/>
    <dbReference type="EnsemblBacteria" id="ABA03284">
        <property type="protein sequence ID" value="ABA03284"/>
        <property type="gene ID" value="Nwi_0016"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0016"/>
    <dbReference type="eggNOG" id="COG0319">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106710_0_0_5"/>
    <dbReference type="OMA" id="HHPGEAR"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00009">
        <property type="entry name" value="Endoribonucl_YbeY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023091">
        <property type="entry name" value="MetalPrtase_cat_dom_sf_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002036">
        <property type="entry name" value="YbeY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020549">
        <property type="entry name" value="YbeY_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46986">
        <property type="entry name" value="PTHR46986"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02130">
        <property type="entry name" value="YbeY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00043">
        <property type="entry name" value="TIGR00043"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01306">
        <property type="entry name" value="UPF0054"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000284256" description="Endoribonuclease YbeY">
        <location>
            <begin position="1"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00009"/>
        </source>
    </evidence>
    <sequence length="190" mass="20879" checksum="713693782FC06B99" modified="2005-10-11" version="1">MPNITCREQPARARRGGEKHIMIRSATPVTEILVVADCWRAEAGAEAVIHRAIATAAGMVDKDTGDAELAIMLTDDAGIRTLNANWRGLDKPTNVLSFPALQPTGPCLPDDPPRMLGDIAIAYETLRREAGDERKTFDHHLSHLAVHGFLHLIGYDHETDGEAEEMESLERQILAQLGIPDPYAPPERMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMU6</accession>
    <name>Q3SMU6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0005"/>
    <dbReference type="EnsemblBacteria" id="ABA03273">
        <property type="protein sequence ID" value="ABA03273"/>
        <property type="gene ID" value="Nwi_0005"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0005"/>
    <dbReference type="eggNOG" id="COG3409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120476_0_0_5"/>
    <dbReference type="OMA" id="AVCWLKN"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01100">
        <property type="entry name" value="APPLE_Factor_XI_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000177">
        <property type="entry name" value="Apple"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14295">
        <property type="entry name" value="PAN_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Apple" evidence="1">
        <location>
            <begin position="51"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="1">
        <location>
            <begin position="134"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="206" mass="22968" checksum="E7C9DB9080754B00" modified="2005-10-11" version="1">MTPIRARPRWGCAALRQVMKVHALKVGLVLLAALLWSSVPAVRPASAQVNFDRPGADYLRVPLRSGDPVDCGLACERDRRCRAWSFSYPNERSETAVCWLKNAVPQRIANRCCVSGVRGAGVIERRIGPVETSTDRSGGDYRNFEIRKDERADADQVCRHACDADSKCRAWTYARPGYAGKAARCFLKKEIKPPHRRPGFTSGVIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN11</accession>
    <name>Q3SN11_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, AbrB family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_3083</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316245.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3083"/>
    <dbReference type="EnsemblBacteria" id="ABA06330">
        <property type="protein sequence ID" value="ABA06330"/>
        <property type="gene ID" value="Nwi_3083"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3083"/>
    <dbReference type="eggNOG" id="COG2002">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158484_8_0_5"/>
    <dbReference type="OMA" id="GCLKYQG"/>
    <dbReference type="OrthoDB" id="1942483at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00966">
        <property type="entry name" value="SpoVT_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="SSF89447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01439">
        <property type="entry name" value="lp_hng_hel_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="9"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="91" mass="9728" checksum="5B5F58ED5E34F2A9" modified="2005-10-11" version="1">MASAEKLTTTVSTKGQVILPSAIRQRREWNAGTRLTVEETPDGVLLKPAPAFAETRPGDVFGVLPSKGGSKTIAEMDAGILAEAKRRHARD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN27</accession>
    <name>TRUA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA pseudouridine synthase A</fullName>
            <ecNumber evidence="1">5.4.99.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridine(38-40) synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridylate synthase I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA-uridine isomerase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">truA</name>
        <name type="ordered locus">Nwi_3066</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:22376"/>
            <dbReference type="Rhea" id="RHEA-COMP:10085"/>
            <dbReference type="Rhea" id="RHEA-COMP:10087"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.12" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316230.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SN27"/>
    <dbReference type="SMR" id="Q3SN27"/>
    <dbReference type="STRING" id="323098.Nwi_3066"/>
    <dbReference type="EnsemblBacteria" id="ABA06314">
        <property type="protein sequence ID" value="ABA06314"/>
        <property type="gene ID" value="Nwi_3066"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3066"/>
    <dbReference type="eggNOG" id="COG0101">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014673_0_2_5"/>
    <dbReference type="OMA" id="FLYGMVR"/>
    <dbReference type="OrthoDB" id="1075262at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106029">
        <property type="term" value="F:tRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02570">
        <property type="entry name" value="PseudoU_synth_EcTruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00171">
        <property type="entry name" value="TruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PTHR11142"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001430">
        <property type="entry name" value="tRNA_psdUrid_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00071">
        <property type="entry name" value="hisT_truA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_1000017125" description="tRNA pseudouridine synthase A">
        <location>
            <begin position="1"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00171"/>
        </source>
    </evidence>
    <sequence length="255" mass="28365" checksum="BC952EFCF18246FB" modified="2005-10-11" version="1">MPRYKLTIEYDGGPFCGWQYQTNGPSVQGALEAAVKAICGDDVRVHGAGRTDAGVHALAQVAHCDIARHFMPDRLRDGLNAHLRPHPIGVLEAEIVPDSFEARFSARRRHYIYRIANRRANLAIDVGRVWRVPRPLDTEAMHAAAQRLLGKHDFTTFRDTECQAKSPEKTLDQLDVIRNGDNVSIITSARSYLHSQVRSMVGSLVWVGHGRWNADDLSGALAARRREACGPVAPPDGLYLARVDYEGSIPLRQDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN29</accession>
    <name>Q3SN29_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptide deformylase</fullName>
            <shortName evidence="2">PDF</shortName>
            <ecNumber evidence="2">3.5.1.88</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Polypeptide deformylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">def</name>
        <name evidence="3" type="ordered locus">Nwi_3064</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H2O + N-terminal N-formyl-L-methionyl-[peptide] = formate + N-terminal L-methionyl-[peptide]</text>
            <dbReference type="Rhea" id="RHEA:24420"/>
            <dbReference type="Rhea" id="RHEA-COMP:10639"/>
            <dbReference type="Rhea" id="RHEA-COMP:10640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:49298"/>
            <dbReference type="ChEBI" id="CHEBI:64731"/>
            <dbReference type="EC" id="3.5.1.88"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="2">Binds 1 Fe(2+) ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the polypeptide deformylase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.88" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3064"/>
    <dbReference type="EnsemblBacteria" id="ABA06312">
        <property type="protein sequence ID" value="ABA06312"/>
        <property type="gene ID" value="Nwi_3064"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3064"/>
    <dbReference type="eggNOG" id="COG0242">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061901_2_0_5"/>
    <dbReference type="OMA" id="VCIQHEI"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042586">
        <property type="term" value="F:peptide deformylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00487">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.45.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00163">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023635">
        <property type="entry name" value="Peptide_deformylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036821">
        <property type="entry name" value="Peptide_deformylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10458">
        <property type="entry name" value="PTHR10458"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01327">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004749">
        <property type="entry name" value="Pep_def"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01576">
        <property type="entry name" value="PDEFORMYLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56420">
        <property type="entry name" value="SSF56420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00079">
        <property type="entry name" value="pept_deformyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="2">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="187" mass="21090" checksum="91D896F1BFEE33F7" modified="2005-10-11" version="1">MTPAGPGITSPCMAIREIIILPDKQLRLVSRPIETVTPEIRKLADDMFETMYDAPGIGLAGIQIAQPLRIVTMDLARRDEEGELTPRPRVFINPEILSASEELSVYEEGCLSIPEYYEAVERPSRVRIRFTDLDGKVHEEDAEGLFATCIQHEIDHLNGVLFVDHLSKLKRDRVMKKFTKAAKFAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN35</accession>
    <name>Q3SN35_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_3058</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316222.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3058"/>
    <dbReference type="EnsemblBacteria" id="ABA06306">
        <property type="protein sequence ID" value="ABA06306"/>
        <property type="gene ID" value="Nwi_3058"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3058"/>
    <dbReference type="eggNOG" id="COG0784">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_72_5"/>
    <dbReference type="OMA" id="NVDCAGI"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021800">
        <property type="entry name" value="DUF3369"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11849">
        <property type="entry name" value="DUF3369"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Response regulatory" evidence="7">
        <location>
            <begin position="28"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="6">
        <location>
            <begin position="360"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="312"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="4">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA06306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="580" mass="63402" checksum="558667962CEC3072" modified="2005-10-11" version="1">MADQDDVLDLIDDSGADRPDASTVRKWKVAVIDDDRAVHEGTRFALSDYSLNGQTLDILSAYSAAEGRELMRAHPDIAAVLLDVIMETDEAGLDLVEFIRNDLRNETVRVILRTGQPGQAPERRVIVDYDINDYKAKTELTADKLFTSLTAALRSYQQLERMVQTRRGLEIIIDAASTLYDFKSMQRLAEGVLTQLASLLNVDCAGILVLRDGGADDDDFSVLAGSGCYRRFSGAEGQASLDCDLKQTVEDAFRRRRHEFVDHRSILYIRTGSGREVVILLQAECDLSDTDRSLVEIFGSRLSIAFDNVILYQQLQDANAQLEDRVAQRTRALMQANRRLSAQWLRLQRANGFKNEILGTVAHDLKNPLGVILGRAEMLAELIGAGSSGDTVTVQINHIRDATKRLIAMVDDLISDAMADAFDITIRREPVDVAALVAEVAEANQPPALNKQQTITVSAPAGHVTMCDQDRMREAIDNLVSNAIKYSPVGGRITLLVDHDAGNTIIRITDEGAGLSPEDLGRLFGRFQRLSAKPTAGESSTGLGLSIVKRIVDMHDGEIKADSGGPGQGSAFTIILPAAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN38</accession>
    <name>Q3SN38_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cold-shock DNA-binding protein family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_3054</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316219.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3054"/>
    <dbReference type="EnsemblBacteria" id="ABA06303">
        <property type="protein sequence ID" value="ABA06303"/>
        <property type="gene ID" value="Nwi_3054"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3054"/>
    <dbReference type="eggNOG" id="COG1278">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117621_4_1_5"/>
    <dbReference type="OMA" id="FICKDQG"/>
    <dbReference type="OrthoDB" id="2034748at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012156">
        <property type="entry name" value="Cold_shock_CspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019844">
        <property type="entry name" value="CSD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002599">
        <property type="entry name" value="Cold_shock_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00050">
        <property type="entry name" value="COLDSHOCK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00352">
        <property type="entry name" value="CSD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CSD" evidence="3">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA06303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="66" mass="7069" checksum="7EC3F3458C41E811" modified="2005-10-11" version="1">MAKGTVKWFNPTKGYGFIQPAGGGKDVFVHISAVERAGLSTLNEGQTVEFEEVANRGKTSAENLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNB3</accession>
    <name>Q3SNB3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Elongation factor G</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_2978</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316150.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2978"/>
    <dbReference type="EnsemblBacteria" id="ABA06228">
        <property type="protein sequence ID" value="ABA06228"/>
        <property type="gene ID" value="Nwi_2978"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2978"/>
    <dbReference type="eggNOG" id="COG0480">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002794_4_2_5"/>
    <dbReference type="OMA" id="DRGHEDH"/>
    <dbReference type="OrthoDB" id="88967at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16262">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01434">
        <property type="entry name" value="EFG_mtEFG1_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03713">
        <property type="entry name" value="EFG_mtEFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009022">
        <property type="entry name" value="EFG_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035649">
        <property type="entry name" value="EFG_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="9">
        <location>
            <begin position="23"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA06228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="696" mass="74264" checksum="99059BFF87E2E199" modified="2005-10-11" version="1">MGQDLRSPQGVSQERGSQGTQASGPRCIALVGPFQSGKTTLLEAILARTGAIPKAGSVDQATSVGDASAEARQHRMGVALTPATTCFMDENYTFIDCPGSIEFAHDMRAAIPAVDAAVVVCEADERKLPQLQIILRELEDLNIPRFLFLNKIDKANKGVRETLATLQQASRTPLLLRQIPVWNGDSISGFVDLALERAFVYREHMASEVVQLDGGDLDREKEARFSMLEKLADHDDALMEQLLEDIAPPQDAVFDDLARELREGRICPVLLGSAARENGVMRLMKALRHEAPGIGETVKRMGVAGSKEALACVFKTVHLQHGGKLSLARVLTGRFDDGAAVQASSGEAGKISGIFAATGAQETKRASAGAGEVVALGKLEAVRTGDTLTTARAAPPSLVSIAPSPPVLAMALAAGDRKDDVKLGQALSKLNEEDPSLGVVHNPQTHDTVLWGQGEMHLRVALERLRDRFGVNVSSHPPAIGYQEAIRKPVSQRGRHKKQSGGHGQFGDVLLEIQPLSRGAGFVFVDRIVGGAVPRNYIGAVEDGVVDGLARGPLGFPVTDVQVTLTDGSYHSVDSSDQAFRTAARIGIAEALPKCQPVLLEPIDVVEIVCPSEATAKVNAILSARRGQILGFDSREGWPGWDCVRATMPEAEIGDLIIELRSATAGAGGFTRQFDRMAEVTGRTADQIIASRRVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNH3</accession>
    <name>Q3SNH3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Integrase, catalytic region</fullName>
            <ecNumber evidence="2">2.7.7.49</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2918</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316098.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06168">
        <property type="protein sequence ID" value="ABA06168"/>
        <property type="gene ID" value="Nwi_2918"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2918"/>
    <dbReference type="HOGENOM" id="CLU_027402_15_1_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13683">
        <property type="entry name" value="rve_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="127"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="316" mass="36569" checksum="49920D96407A8C67" modified="2005-10-11" version="1">MGQVLHGSATTTEAIRRAIQNSEESLRALSKRYGINQKTVAKWKKRTSVADLPTGPKDPRSTVLSSEEEAIIVAFRKHTLLPLDDCLYALQPTIPHLSRSSLHRCLHRHGISRLPEVEGDKPIRKTFRSYPIGYFHIDIAEVQTAEGKLRLFVAIDRTSKFAYAELHQEAGKMVAAQFLRNLIVAVPYAIHTVLTDNGIQFTNHARHKYAFHHIFDRVCDENGIEHRLTRINHPWTNGQVERMNRTIKDATVKRFHYDNHDQLRRHLQDFIKAYNFGRRLKTLKGLTPYEFICKRWTSEPDRFIIDPIHQMPGLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNI6</accession>
    <name>Q3SNI6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nickel/cobalt efflux system</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_2905</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the NiCoT transporter (TC 2.A.52) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316085.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2905"/>
    <dbReference type="EnsemblBacteria" id="ABA06155">
        <property type="protein sequence ID" value="ABA06155"/>
        <property type="gene ID" value="Nwi_2905"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2905"/>
    <dbReference type="eggNOG" id="COG2215">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058605_0_2_5"/>
    <dbReference type="OMA" id="GLLLCWR"/>
    <dbReference type="OrthoDB" id="1515392at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015099">
        <property type="term" value="F:nickel cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011541">
        <property type="entry name" value="Ni/Co_transpt_high_affinity"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03824">
        <property type="entry name" value="NicO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0533">Nickel</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="122"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="274"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="308"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA06155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="381" mass="39783" checksum="6EA0A8953FDDE884" modified="2005-10-11" version="1">MADGHGHQCPYAAKVAIFTCALLLVAGLMDAALAQSLSLGSRQPAAASSSAGFFGWIFSQQAAIYRSLSALVRASKTDGSAIWGLLGFSFLYGVFHAAGPGHGKAVISSYLVANNETWKRGVTLSFASAMLQAMVAIAIVAIGAIVLEVTAKAMGDTVRVIEIFSYGLVVLIGARLLWVKGRSFLRSCREIRRARSLGAAAVAAEHRVPDLSKPAVDCGCRHHDGQRHGHLRHDHSAHKDCDPACDHDHASGCGHAHGPEPEDLAGPGGWRRGFAAVLAVGARPCSGAIIILVFALSQDMFWTGIGSALMMGAGTAITIAAIATLAVCARNLAGRMTDARPGRGALVMKGIEVGAAAMVIVVGLFLLTGFMASEQLWMFNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNJ1</accession>
    <name>Q3SNJ1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2900</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316080.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2900"/>
    <dbReference type="EnsemblBacteria" id="ABA06150">
        <property type="protein sequence ID" value="ABA06150"/>
        <property type="gene ID" value="Nwi_2900"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2900"/>
    <dbReference type="eggNOG" id="ENOG5032R4T">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1766581_0_0_5"/>
    <dbReference type="OMA" id="CEKECRK"/>
    <dbReference type="OrthoDB" id="1635052at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030913">
        <property type="entry name" value="TAT_Cys_rich"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04401">
        <property type="entry name" value="TAT_Cys_rich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229051" evidence="1">
        <location>
            <begin position="30"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="146" mass="15218" checksum="1BB1E9B3F8302E1E" modified="2005-10-11" version="1">MHRREFVTAIGTAAAVVSAAQAFAVTADAATGESMHPAKYKALEEAIAHCISAGDACMRHCLGMLSMKDVSMAGCTNATYQVIAGCTALRTLAAVNSHHVPALGKVVAQMCTDCQKECEKFPDVAECKSCGDACKTCAEECRKVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNN5</accession>
    <name>Q3SNN5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GCN5-related N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2856</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316044.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2856"/>
    <dbReference type="EnsemblBacteria" id="ABA06106">
        <property type="protein sequence ID" value="ABA06106"/>
        <property type="gene ID" value="Nwi_2856"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2856"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101288_3_0_5"/>
    <dbReference type="OMA" id="FNCGIEA"/>
    <dbReference type="OrthoDB" id="2055010at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="9"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="170" mass="19092" checksum="40A492862B82077A" modified="2005-10-11" version="1">MGKGGDELLRVEVLGPHHDRSGFESGIEPLDRYFRTQASQDAKKNMAAPFVLVLPDRAIGGYYTLSSTALKLAELPAQATRKLPRYPSVPATLIGRLAVDRRHQGKGYGRFLLADALYRALRSEIAAFAVIVDAKDENARRFYERESFLPLPDQPMKLFRPMGDIEKLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNR6</accession>
    <name>Q3SNR6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase, IS4 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2822</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2822"/>
    <dbReference type="EnsemblBacteria" id="ABA06075">
        <property type="protein sequence ID" value="ABA06075"/>
        <property type="gene ID" value="Nwi_2822"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2822"/>
    <dbReference type="eggNOG" id="COG3293">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055261_9_3_5"/>
    <dbReference type="OMA" id="WRIESAF"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025668">
        <property type="entry name" value="Tnp_DDE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13586">
        <property type="entry name" value="DDE_Tnp_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tnp_DDE_dom" evidence="1">
        <location>
            <begin position="36"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="127" mass="14669" checksum="AA64D76815C5C619" modified="2005-10-11" version="1">MDACGLPIVLKITEGQAHDGRSAQDMIDTVERGDVLLADRAYDSNALRQTLAARGARANVKPMPNRVAALQFNRRLYRKRNLVERFFNKIKHYRAVATRYDKRDDNFLTSIKLASIRIWLRFNESMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNV2</accession>
    <name>Q3SNV2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2786</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06039">
        <property type="protein sequence ID" value="ABA06039"/>
        <property type="gene ID" value="Nwi_2786"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2786"/>
    <dbReference type="eggNOG" id="ENOG503171Q">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1584753_0_0_5"/>
    <dbReference type="OMA" id="DGHFKNQ"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="201" mass="20571" checksum="F10D88651377E209" modified="2005-10-11" version="1">MRRRIRPASTGGLPSPDNSVSRNDVMKTAKTRAAAPKAEKTSRPPANAKASAKTPSRTAAKATAKAAAKPASEKGKAAGPAEVVESGGTAAPRAADKPVMTAPRDALTLPPKPAAAITPPIPAPVAAPPTPAATTIKPVSGPAARADVPPDAGFTLRVDGHFKNQFETLAQATQAATDLKSRFPILRIEIYDAANKARLPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP08</accession>
    <name>Q3SP08_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2730</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315929.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2730"/>
    <dbReference type="EnsemblBacteria" id="ABA05983">
        <property type="protein sequence ID" value="ABA05983"/>
        <property type="gene ID" value="Nwi_2730"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2730"/>
    <dbReference type="eggNOG" id="COG1692">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068238_0_0_5"/>
    <dbReference type="OMA" id="NHIWDKK"/>
    <dbReference type="OrthoDB" id="1127353at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005235">
        <property type="entry name" value="YmdB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36303">
        <property type="entry name" value="PTHR36303"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13277">
        <property type="entry name" value="YmdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004789">
        <property type="entry name" value="DR1281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00282">
        <property type="entry name" value="TIGR00282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="39"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR004789-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR004789-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="273" mass="29413" checksum="EB01B8F83D431823" modified="2005-10-11" version="1">MRILFIGDVVGRTGRTAINDYLPELVTAWKLDLVVVNGENAAGGFGITEAIYQELLDAGADAITLGNHAWNQKEALVFIERAPRLVRPLNFPPGTPGRGAAMIDAKDGRRALVINAMARIFMEPLNDPFHAIEYELDACRLREAADAIIVDFHGEATSEKQSMGYFCDGRVSLVVGTHTHVPTADHRILSGGTAYMSDAGMTGDYDSVIGMQKEEPLRRFTTGIPSSRFEPAAGAATLSGVAVETDDATGLALKIAPVRIGGRLERAEPSFWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP52</accession>
    <name>Q3SP52_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glutathione-dependent peroxiredoxin</fullName>
            <ecNumber evidence="3">1.11.1.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2686</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a hydroperoxide + 2 glutathione = an alcohol + glutathione disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62632"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="EC" id="1.11.1.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxiredoxin family. Prx5 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.27" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315885.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2686"/>
    <dbReference type="EnsemblBacteria" id="ABA05939">
        <property type="protein sequence ID" value="ABA05939"/>
        <property type="gene ID" value="Nwi_2686"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2686"/>
    <dbReference type="eggNOG" id="COG0678">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072440_1_2_5"/>
    <dbReference type="OMA" id="GYINHPK"/>
    <dbReference type="OrthoDB" id="892697at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03013">
        <property type="entry name" value="PRX5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037944">
        <property type="entry name" value="PRX5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013740">
        <property type="entry name" value="Redoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10430">
        <property type="entry name" value="PTHR10430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08534">
        <property type="entry name" value="Redoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1 3" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="3" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="4">
        <location>
            <begin position="3"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU366011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="161" mass="17347" checksum="634F2AB1F9718149" modified="2005-10-11" version="1">MTIKVGDCLPNATFRIMTEDGVLTKSTDDIFKSKKVALFAVPGAYTGTCHKQHLPSIFASANAIKGKGVNEIAIVSVNDVFVMNAWKRDTDQRNEATFLADGNAEFAKAIDMTFDGSEKGLGIRSKRYSMLVEDGVVKTLNVEDSPGKVEVSGGDKLLGQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP54</accession>
    <name>Q3SP54_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">AMP-dependent synthetase and ligase</fullName>
            <ecNumber evidence="5">6.2.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2684</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.3" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2684"/>
    <dbReference type="EnsemblBacteria" id="ABA05937">
        <property type="protein sequence ID" value="ABA05937"/>
        <property type="gene ID" value="Nwi_2684"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2684"/>
    <dbReference type="eggNOG" id="COG0318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_9_5"/>
    <dbReference type="OMA" id="YIMPKFD"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="260"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding" evidence="3">
        <location>
            <begin position="37"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="4">
        <location>
            <begin position="479"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="567" mass="62040" checksum="7F934E4A84A41158" modified="2005-10-11" version="1">MGSPGRHMDRIWLRQYPAGVPADIDASQYASLIELLEESFARFSDRKAFICMDTAMTYREIDETSRALGAYLQSLGLKKGARVALMMPNVLQYPISTVAVLRAGYAAVNVNPLYTPHELEYQLKDSGAEAIIVLENFAATVQQAIGKTAVRHVIVGSMGDLLGFKGLIVNFVVRHIKKMVPPSSIPHAVKFNDALAAGRGMALARPELAPDDVAFLQYTGGTTGVSKGATLLHRNILANMLQNDAWLQPVLKKPPVIDQLFIVCALPLYHIFALTACFMLGMRAGGVNLLIPNPRDIPGLVKELRKYQINCFPAVNTLYNALLNAPGFEQVDFSKLRASIGGGMAVQKAVAERWLKVTGCPLLEGYGLSETSPTLTCNPADGDKYTGSIGLPLPSTYISIRDNDGREMPLGEAGEICASGPQVMPGYWNRQDETAKVMTADGFFRTGDIGVMSTDGYTRLVDRKKDMIVVSGFNVYPNEIEDVIASHPGVLECAVIGVKDERSGETVKAFVVRKDPDLTREEIIKFARTELTNYKVPKQIEFRDSLPKTNVGKILRRALRDENKTAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP69</accession>
    <name>Q3SP69_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_2669</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC-4 integral membrane protein family. LolC/E subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315870.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2669"/>
    <dbReference type="EnsemblBacteria" id="ABA05922">
        <property type="protein sequence ID" value="ABA05922"/>
        <property type="gene ID" value="Nwi_2669"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2669"/>
    <dbReference type="eggNOG" id="COG0577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012341_0_1_5"/>
    <dbReference type="OMA" id="PQSFHWT"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038766">
        <property type="entry name" value="Membrane_comp_ABC_pdt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30287">
        <property type="entry name" value="PTHR30287"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="240"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="283"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="339"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="384"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="411"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="457"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="684"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="738"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="785"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="8">
        <location>
            <begin position="270"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="MacB_PCD" evidence="9">
        <location>
            <begin position="462"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="8">
        <location>
            <begin position="720"/>
            <end position="809"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA05922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="820" mass="88094" checksum="4C7B5907DD18728B" modified="2005-10-11" version="1">MKQGYWVLAVLLSHWRRHPLQLMTLMVGLISATALWSGVEALNHQARASYDRAAAVFGGPRTAMLVGQRSSTFSQQLFVDLRRAGWRVSPVVEGRVRVQGRPLRLLGIEPVTFPAQAGSIPAIGAPDLKSFIAPPGEALVAPETLIALAGTEGAGLRTDDGASLPPLRAHAQLAPDVVIVDIGAAQGILHLQGQLSRLLVADARSRSPPLEAVTGDMLRYVAADAGTDLEHLTDSFHLNLSAFGLLSFLVGLFIVYSAIGLAFEQRMPMFRTLRACGVSSRRLNGLLLIELLVLAIAGGLVGLVCGYLIAAGLLPNVAASLQGLYGARVSGALALRPEWWVAGLAMSAIGTLAASATSLLKVIRLPVLAAAQPYAWRQSQRQWLGWQGVTAAAMLVAAIIVLILGESLISGFVVLGLLLVGAALVLPVLLAFLLRIGERSSRRPLVLWFWADSRQQLPGLSLALMALLLALAVNVGVGTMVESFSKTFTAWLDGRLAADVYVNAMSEAQARDIEASLRARQEVTAILPSGRAETGIGGRPVEILGLADHATYRDLWPLLESRPNAWDDLKSGEGALVSEQLARRLKLRPGDRLDVGAPTGDWSLRIAAIYADYGNPKGQIAVNIDAFTDRFPGLPRTRFGLRVSPGKIAPLIDDMRKAFHLDESRLSDQSTLKMESKGIFNRTFAVTAALNTFTLGVAGVALLTSLLTLSHSRLPQLAPLWALGVTRRQLAGIEFLKTVFIAFITAMLALPLGLLVAWCLIAVVNVRAFGWRLPFHVFPLHLAELVVVAMLASLLAMLIPLLRLTRMPPAALIRIFSNER</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP93</accession>
    <name>Q3SP93_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Gluconokinase</fullName>
            <ecNumber evidence="4 9">2.7.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_2645</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>ATP + D-gluconate = 6-phospho-D-gluconate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19433"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18391"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58759"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate acid metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the gluconokinase GntK/GntV family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.12" evidence="4 9"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315848.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2645"/>
    <dbReference type="EnsemblBacteria" id="ABA05898">
        <property type="protein sequence ID" value="ABA05898"/>
        <property type="gene ID" value="Nwi_2645"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2645"/>
    <dbReference type="eggNOG" id="COG3265">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077168_4_1_5"/>
    <dbReference type="OMA" id="ENFIQVS"/>
    <dbReference type="OrthoDB" id="1843451at2"/>
    <dbReference type="UniPathway" id="UPA00345"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046316">
        <property type="term" value="F:gluconokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02021">
        <property type="entry name" value="GntK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031322">
        <property type="entry name" value="Shikimate/glucono_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006001">
        <property type="entry name" value="Therm_gnt_kin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43442">
        <property type="entry name" value="PTHR43442"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01202">
        <property type="entry name" value="SKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01313">
        <property type="entry name" value="therm_gnt_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9 10" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA05898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="172" mass="18881" checksum="4DE7371BE5FEED77" modified="2005-10-11" version="1">MNAGGGAAFRALIVMGVSGSGKSVIADALGRRLGWIVEDADRFHAKNSIDKMRDGIALTDDDRRPWLRAVAAEIGRRRADGTSVIMACSALKRAYRDILVQGSSDTRIIYLRGDKDLIASRLKTRSEHFMPQGLLASQFATLEEPTEDERPIVVDIDATIKDITERIIIALD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPD1</accession>
    <name>Q3SPD1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein-disulfide isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2607</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be required for disulfide bond formation in some proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the thioredoxin family. DsbA subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315810.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2607"/>
    <dbReference type="EnsemblBacteria" id="ABA05860">
        <property type="protein sequence ID" value="ABA05860"/>
        <property type="gene ID" value="Nwi_2607"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2607"/>
    <dbReference type="eggNOG" id="COG1651">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_47_5_5"/>
    <dbReference type="OMA" id="APEDRYF"/>
    <dbReference type="OrthoDB" id="1678187at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13462">
        <property type="entry name" value="Thioredoxin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229204" evidence="3">
        <location>
            <begin position="22"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="4">
        <location>
            <begin position="28"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="220" mass="23994" checksum="9BA11E377D2779BF" modified="2005-10-11" version="1">MIITRRAFNAALSLTGLAAVAGLSPWRFLGVAQAQNATAADVAKPVSLPDMALGPKDAAVTITEYASMTCPHCARFAEDVFPKIKTEYIDTNKIRYVFREFPLDIKAAAGAMLARCIAKDDAGKYFAVIDTLFKSQDTWTGSKTTESLKLIGKQTGLTEGEVENCLKDQALLDKIAADQKYANEVLKVNSTPSFFINGDMVKGEIAFEDFKNKIDPLLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPG1</accession>
    <name>Q3SPG1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">META domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2577</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315781.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05830">
        <property type="protein sequence ID" value="ABA05830"/>
        <property type="gene ID" value="Nwi_2577"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2577"/>
    <dbReference type="eggNOG" id="COG3187">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130281_0_0_5"/>
    <dbReference type="OMA" id="LELWCKG"/>
    <dbReference type="OrthoDB" id="1873893at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005184">
        <property type="entry name" value="DUF306_Meta_HslJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038670">
        <property type="entry name" value="HslJ-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03724">
        <property type="entry name" value="META"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229025" description="META domain-containing protein" evidence="1">
        <location>
            <begin position="27"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="META" evidence="2">
        <location>
            <begin position="47"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="135" mass="14048" checksum="42DF0FF7C0F1B3EF" modified="2005-10-11" version="1">MAAGIGFLSGVVTLALGAACAWPAFAQGGFPFGLEMTLDAPPRPGSKRVPSLDIGDSGGVKLELWCKGGKGQFSVAGETVIFAAGPLEDRSCPPEKAEADDELVAALSAADTWERQGDEITFVGPTRLRFHLNTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPN7</accession>
    <name>Q3SPN7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribose-phosphate pyrophosphokinase</fullName>
            <shortName evidence="6">RPPK</shortName>
            <ecNumber evidence="6">2.7.6.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">5-phospho-D-ribosyl alpha-1-diphosphate</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Phosphoribosyl diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Phosphoribosyl pyrophosphate synthase</fullName>
            <shortName evidence="6">P-Rib-PP synthase</shortName>
            <shortName evidence="6">PRPP synthase</shortName>
            <shortName evidence="6">PRPPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">prs</name>
        <name evidence="8" type="ordered locus">Nwi_2501</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib-5-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + D-ribose 5-phosphate = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Binds 2 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ribose-phosphate pyrophosphokinase family. Class I subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2501"/>
    <dbReference type="EnsemblBacteria" id="ABA05754">
        <property type="protein sequence ID" value="ABA05754"/>
        <property type="gene ID" value="Nwi_2501"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2501"/>
    <dbReference type="eggNOG" id="COG0462">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033546_4_0_5"/>
    <dbReference type="OMA" id="FGWARQD"/>
    <dbReference type="UniPathway" id="UPA00087">
        <property type="reaction ID" value="UER00172"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009165">
        <property type="term" value="P:nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009156">
        <property type="term" value="P:ribonucleoside monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00583_B">
        <property type="entry name" value="RibP_PPkinase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000842">
        <property type="entry name" value="PRib_PP_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037515">
        <property type="entry name" value="Rib-P_diPkinase_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="PTHR10210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00114">
        <property type="entry name" value="PRPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pribosyltran_N" evidence="7">
        <location>
            <begin position="23"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Ribose-5-phosphate binding" evidence="6">
        <location>
            <begin position="241"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="55"/>
            <end position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="114"/>
            <end position="115"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA05754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="331" mass="35466" checksum="B14259C1FA142472" modified="2005-10-11" version="1">MLNVVSTTSRGDASMSAKNGAVKLVAGNSNPVLAREIGRWLGLELTKASVRRFADNEIFVEVLENVRGSDVYVIQSTSYPTNDHLMELLIITDALRRASARRITAVIPYFGYARQDRKVGSRSPISAKLVANLITRAGMDRVLTLDLHAGQIQGFFDIPTDNLYASPVMVRDIKERFELGSLMVVSPDVGGVVRARGLAKRISAPLAIIDKRRERAGESEVMNVIGDVAGYTCILIDDIVDSGGTLVNAADALLANGARDVYAYITHGVLSGGAAARIAGSKLKELVITDSIQPTDAVNKTGNIRLLSIAGLIGEAIRRTAAEQSVSSLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPS9</accession>
    <name>Q3SPS9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2459</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315667.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2459"/>
    <dbReference type="EnsemblBacteria" id="ABA05712">
        <property type="protein sequence ID" value="ABA05712"/>
        <property type="gene ID" value="Nwi_2459"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2459"/>
    <dbReference type="eggNOG" id="ENOG503353M">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_533035_0_0_5"/>
    <dbReference type="OMA" id="QNADIGW"/>
    <dbReference type="OrthoDB" id="308080at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025515">
        <property type="entry name" value="DUF4403"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14356">
        <property type="entry name" value="DUF4403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="518" mass="54716" checksum="9CC1579ACD44D32D" modified="2005-10-11" version="1">MRSGFKFKTILLGIGILAVSFFVSLKAMDWLSPHGIDKPALAALPPLPPVNRSSSIVVPVKIPLTLIRDAAERAAPHHFAGKADNPAAQILQNADIGWTASRGAISATGANNALSLSTPLTGAVKVTGSLSANAQDAVGNALAGVLGGDIAKHVGRVNIKNLNANAEIKGNVVMTAQPTLAGNWRIDPGITAQVNLGDTNLSVAGARLNVPSQVKPVIDKAVNEQIAVLQQRIRDDPALERDARRQWAQMCRSIPLQDPAGMPQLHLELKPVRAIAAQPRIDARNVTLTIGVEAETRITTAATRPDCPFPATLALEPATTGRLNIGVPIDMPFTDINKIIEAQFAGKTFPEDGSGAADVTVKQVSVAAAGDQLLISLLVHAKEKKSWFGFGGEATIHIWGRPVLDQAQQIMRLKDIRLAVESEAAFGLLGAAAQTALPYLESALAERAVVDLKPFVSDAREKIAAVIADFQKSEDRAKVTAEISRLNLSSIAFDSTTLRVIAEAAGSVNVELKTLPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPZ1</accession>
    <name>Q3SPZ1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-acetylneuraminate synthase</fullName>
            <ecNumber evidence="2">2.5.1.56</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2396</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.5.1.56" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2396"/>
    <dbReference type="EnsemblBacteria" id="ABA05650">
        <property type="protein sequence ID" value="ABA05650"/>
        <property type="gene ID" value="Nwi_2396"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2396"/>
    <dbReference type="eggNOG" id="COG2089">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040465_0_0_5"/>
    <dbReference type="OMA" id="WKRMEFT"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050462">
        <property type="term" value="F:N-acetylneuraminate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016051">
        <property type="term" value="P:carbohydrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006190">
        <property type="entry name" value="AFP_Neu5c_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036732">
        <property type="entry name" value="AFP_Neu5c_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020007">
        <property type="entry name" value="N-AcNeuraminate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013132">
        <property type="entry name" value="Neu5Ac_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03102">
        <property type="entry name" value="NeuB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51269">
        <property type="entry name" value="SSF51269"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03569">
        <property type="entry name" value="NeuB_NnaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50844">
        <property type="entry name" value="AFP_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AFP-like" evidence="1">
        <location>
            <begin position="319"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="371" mass="40033" checksum="A7EB333B53D7350C" modified="2005-10-11" version="1">MSSAPRKRSDFMLQRARIIAEAGVNHNGDLARALALVDTAARAGADIIKFQSFRADKLATPAAAKASYQQATTGGGQSQLEMLRSLELSESEEREIAAACAAANIGYLSTPFDADSATHLVRHVGVSAFKVGSGDLTNAPLLLHLARFRLPVILSTGMGTLAEIEQALGVIAFGYFREEAAPSTSRAFSEVLLERRAWSMLREKVTLLHCTTEYPAAAHSINLRAMASLREAFGLEVGFSDHSVGIHLAVAAVALGATVIEKHFTLDRNLPGPDHRASLEPDELTAMIAAIRDVESAMGDGRKIPAPEELANRSVARRSLVVTRRVCRGERFSADNLGVKRPGNGVSPMEFWTYLDQVAPRDYEVDEAIEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ23</accession>
    <name>Q3SQ23_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ErfK/YbiS/YcfS/YnhG</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2364</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YkuD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315580.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2364"/>
    <dbReference type="EnsemblBacteria" id="ABA05618">
        <property type="protein sequence ID" value="ABA05618"/>
        <property type="gene ID" value="Nwi_2364"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2364"/>
    <dbReference type="eggNOG" id="COG1376">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096102_0_0_5"/>
    <dbReference type="OMA" id="YSKEFDD"/>
    <dbReference type="OrthoDB" id="975147at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071972">
        <property type="term" value="F:peptidoglycan L,D-transpeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16913">
        <property type="entry name" value="YkuD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005490">
        <property type="entry name" value="LD_TPept_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038063">
        <property type="entry name" value="Transpep_catalytic_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03734">
        <property type="entry name" value="YkuD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141523">
        <property type="entry name" value="SSF141523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229192" evidence="5">
        <location>
            <begin position="23"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="YkuD" evidence="6">
        <location>
            <begin position="26"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="148"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="201"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="270" mass="29799" checksum="F9F7F363A5D45917" modified="2005-10-11" version="1">MRRFLVPVAGLILLAAGGVADAKVSILVDKDNQRMTVSVNGVERYQWPVSSGNPSYETPNGKFQAFRMEADHFSKEFDDAPMPHSIFFTKAGHAIHGTLSEGSLGVPVSHGCVRISRANASTLFSLVKQEGVLNTTVTLTGSSRVALARNPRGKRKTDVAGRDPGPAYQSYDAAGNPVDLTPRQIAPGWQPQAYVRGADGYMYPADGRSNDPRYPDPRSYRRHYGAQNVPPQQHSYYGDRNEYGYAQPYQPQVYAPQPYRPRGLFSDFDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ26</accession>
    <name>Q3SQ26_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter, ATPase subunit</fullName>
            <ecNumber evidence="7">3.6.3.25</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2361</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in beta-(1--&gt;2)glucan export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.25" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315577.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2361"/>
    <dbReference type="EnsemblBacteria" id="ABA05615">
        <property type="protein sequence ID" value="ABA05615"/>
        <property type="gene ID" value="Nwi_2361"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2361"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_5"/>
    <dbReference type="OMA" id="PVMFMDE"/>
    <dbReference type="OrthoDB" id="1926714at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="5"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="306" mass="33554" checksum="6EDCC06A3A94D2B8" modified="2005-10-11" version="1">MSSMISISGLSKAYASGFMALKSIDLDIKRGEIFALLGPNGAGKTTLISIICGITNPSAGQVLVDGRDIRRDYRATRAMIGLVPQELHTDAFETVWATVSFSRGLFGKPRNPTHIEKVLKDLSLWDKKDSKIVTLSGGMKRRVMIAKALSHEPQVLFLDEPTAGVDVELRKAMWGVVRTLRASGVTIILTTHYIEEAEEMADRVGVINKGEIILVDEKTALMQKLGKKRLKLHLQGKVEAIPATLAAYNLELAADGRQVIYTYDTRSEHTGITSLLGDLRAAGIRFSDLDTTQSSLEEIFVSLVRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ45</accession>
    <name>Q3SQ45_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Acetolactate synthase small subunit</fullName>
            <shortName evidence="8">AHAS</shortName>
            <shortName evidence="8">ALS</shortName>
            <ecNumber evidence="8">2.2.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Acetohydroxy-acid synthase small subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_2342</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the conversion of 2 pyruvate molecules into acetolactate in the first common step of the biosynthetic pathway of the branched-amino acids such as leucine, isoleucine, and valine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>H(+) + 2 pyruvate = (2S)-2-acetolactate + CO2</text>
            <dbReference type="Rhea" id="RHEA:25249"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="2.2.1.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 8">Dimer of large and small chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 8">Belongs to the acetolactate synthase small subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.6" evidence="8"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315560.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2342"/>
    <dbReference type="EnsemblBacteria" id="ABA05596">
        <property type="protein sequence ID" value="ABA05596"/>
        <property type="gene ID" value="Nwi_2342"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2342"/>
    <dbReference type="eggNOG" id="COG0440">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055003_1_2_5"/>
    <dbReference type="OMA" id="EPYGIKE"/>
    <dbReference type="OrthoDB" id="1500398at2"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00055"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00059"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990610">
        <property type="term" value="F:acetolactate synthase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04878">
        <property type="entry name" value="ACT_AHAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004789">
        <property type="entry name" value="Acetalactate_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019455">
        <property type="entry name" value="Acetolactate_synth_ssu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039557">
        <property type="entry name" value="AHAS_ACT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30239">
        <property type="entry name" value="PTHR30239"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10369">
        <property type="entry name" value="ALS_ss_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00119">
        <property type="entry name" value="acolac_sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACT" evidence="9">
        <location>
            <begin position="21"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU368092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA05596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="180" mass="19859" checksum="CB694019F3FE7176" modified="2005-10-11" version="1">MNQPASAYFLEERRDPKETHTLSVLVANEPGVLARVIGLFSGRGYNIESLTVSETESKKHVSRITIVTTGTPMVIQQIKHQLDRMIPVYQVVDMTLTGRSIERELAMVKVKGAGDQRIEAMRLAEVFRARVVDATNESFVFEITGGTSKINQFVDLMRPIGLVEVSRTGVAAIGRGPEGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ88</accession>
    <name>Q3SQ88_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Carrier domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2299</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315520.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2299"/>
    <dbReference type="EnsemblBacteria" id="ABA05553">
        <property type="protein sequence ID" value="ABA05553"/>
        <property type="gene ID" value="Nwi_2299"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2299"/>
    <dbReference type="eggNOG" id="COG0236">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052637_0_0_5"/>
    <dbReference type="OMA" id="QNPFPHY"/>
    <dbReference type="OrthoDB" id="470772at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046047">
        <property type="entry name" value="DUF6005"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19468">
        <property type="entry name" value="DUF6005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="SSF47336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="2">
        <location>
            <begin position="4"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="1">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="436" mass="49223" checksum="BC9EAD90D13F0A47" modified="2005-10-11" version="1">MTREDIVASIRAVLAEQMNNTHLDGFGPHSRLNADLYLDSVLLLDLFLHLELKHGLVISEEMVTGPDIETVDDLASLLAGARTTSDVAANASETAVPDVHAEEYPDIKVHCFVSCLCDAVKAAGLDHRLFYFSVWDADFAVSTRHELLYHTPEISHDFFRRWFHRLYGVQVTEWYDHSASKQANLEKLIGLVAARRPTESVMVMLDMFHLPERENKFNQNPFPHYLMLEATEDPEVWQVRDPDFRWEGPIARERLLNAVAQPSVAGGYIFDRQAVAPPLAADIRDFFLACFISGDNPLIRNVREIVAAHVEGRAGLDVSDLKTALRELPVIAIRKWAYEHGFAYFWRALNLPNDEFLHWCDKIEELAVGLKGLHFVILRLAQTGDHAHAADVFAALDKLDALETAIKARLETVFFNWCADQGWKPASTPECLSAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQC3</accession>
    <name>Q3SQC3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF2147 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2264</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2264"/>
    <dbReference type="EnsemblBacteria" id="ABA05518">
        <property type="protein sequence ID" value="ABA05518"/>
        <property type="gene ID" value="Nwi_2264"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2264"/>
    <dbReference type="eggNOG" id="COG4731">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108869_1_1_5"/>
    <dbReference type="OMA" id="GFLCGGE"/>
    <dbReference type="OrthoDB" id="1848240at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019223">
        <property type="entry name" value="DUF2147"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09917">
        <property type="entry name" value="DUF2147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229198" description="DUF2147 domain-containing protein" evidence="2">
        <location>
            <begin position="39"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2147" evidence="3">
        <location>
            <begin position="43"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="213" mass="22321" checksum="6403644096E00338" modified="2005-10-11" version="1">MLQRSRHPMRILDYCRTSAMTSLVAATVMTASSGVAGAADPTGDWRVAEGVANIRVAECNGSMWGVVAWEKKPGGVDRNNPDASKRSRPTLGMPILLDMKKKADKDAWEGQVYNAKDGKTYESTIKLASPDQLEIEGCVLGFLCGGETWTRVGPPISTSASNAPGATASKTGTAKTAKPHKTSSKNKASTTGTASDDPVGDICLLPDIAGATH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQD4</accession>
    <name>Q3SQD4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AcetylCoA_hyd_C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2253</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315475.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2253"/>
    <dbReference type="EnsemblBacteria" id="ABA05507">
        <property type="protein sequence ID" value="ABA05507"/>
        <property type="gene ID" value="Nwi_2253"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2253"/>
    <dbReference type="eggNOG" id="COG0427">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_442578_0_0_5"/>
    <dbReference type="OMA" id="CSEMFVN"/>
    <dbReference type="OrthoDB" id="319106at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1080.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026888">
        <property type="entry name" value="AcetylCoA_hyd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038460">
        <property type="entry name" value="AcetylCoA_hyd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13336">
        <property type="entry name" value="AcetylCoA_hyd_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AcetylCoA_hyd_C" evidence="1">
        <location>
            <begin position="334"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="615" mass="66921" checksum="123E0E69B03749F1" modified="2005-10-11" version="1">MPRIYTDPAAVVDDIIRETGPRLTVGLPLGLGKANHIANALYRRAAADSDLDLTFFAALTLEKPRPSNEMERRFIAPVIERLFGEWPDLDYASALHKGDLPPNIKIIEFFFLAGKWLRNTYAQQHYVAANYTHAASYILDHGLNVITQLVAKRVVGGETRYSLSCNTDTTLDLLRARAAGRASFKLVGQVNSQLPFMPGAGDLPASEFSAVLDSPATDFPLFAPPSEPVSDTKYAIGLHTAGLIRDGGSLQIGIGQTSDALAQSLILRHRDNARFREIATRLAPDRTMAETAPFAIGLYGVSEMVFEAFLGLIKAGVLKREVDGAVLHGAFFLGSQSFYRALREMTPDELSRIQMTAVSFTNDLYGDEAGKRHARVDARFVNNTMMATLMGAAVSDGLENGQVVSGVGGQYNFVAQAFALQGARSILTLEASRREKSGVVSNIRWAYGHATVPRHLRDIVVTEYGVADLRGKSDADVIAAMLNVADSRFQAELMRQAKDAGKLPKSHEIPAVHRENFAGRIKAALRPAHAAGLLPRFPFGSDFTEVEQRLIPALETLRDASRSPLSLMGLLMKGLRPGDVSTQPALARMGLDHPTTLSDRLYRALLNGALKESGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQI4</accession>
    <name>Q3SQI4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">AAA ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2203</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315426.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2203"/>
    <dbReference type="EnsemblBacteria" id="ABA05457">
        <property type="protein sequence ID" value="ABA05457"/>
        <property type="gene ID" value="Nwi_2203"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2203"/>
    <dbReference type="eggNOG" id="COG0714">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051820_1_0_5"/>
    <dbReference type="OMA" id="ALYEWKY"/>
    <dbReference type="OrthoDB" id="489714at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_5" evidence="1">
        <location>
            <begin position="54"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="314" mass="34833" checksum="FCB577E6A599ADED" modified="2005-10-11" version="1">MRDPRRAGPDLMIAESALPSSVDATLDLLAKHGYFAERSLATVVFLSLRMARPLFVEGEAGVGKTEIAKVLSAALGRRLIRLQCYEGLDLASAAYEWNSHAQLIAIRLAEAAGDTDRERLSSDIFAERYLIKRPLLQALEPDVRGPPVLLIDELDRADEAFEAFLLEILGDFQVTIPEFGTVRAAPPPIVIATSNRTREIHDALKRRCLYHWVDYPSAERELAIVRSRVPGISAALSQQVVGFVQALRNRDFYKNPGVAETIDWAMALTELDTRLLTPQIVGDTIGALLKYQDDISRMQDDVVQNLLKEAATDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQJ3</accession>
    <name>Q3SQJ3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PMT_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_2194</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315417.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2194"/>
    <dbReference type="CAZy" id="GT83">
        <property type="family name" value="Glycosyltransferase Family 83"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05448">
        <property type="protein sequence ID" value="ABA05448"/>
        <property type="gene ID" value="Nwi_2194"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2194"/>
    <dbReference type="eggNOG" id="COG1807">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016165_1_0_5"/>
    <dbReference type="OMA" id="EAQYWFW"/>
    <dbReference type="OrthoDB" id="854536at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038731">
        <property type="entry name" value="RgtA/B/C-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13231">
        <property type="entry name" value="PMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="51"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="277"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="338"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="PMT_2" evidence="9">
        <location>
            <begin position="52"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA05448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="498" mass="54222" checksum="7D9D6729DFD7BC08" modified="2005-10-11" version="1">MRLAGTWSVRAVVLTVLALVALRLVCAALTPLTFDEAYYWTWSKHLAGGYYDHPPLVAVVIRLGAMIAGDTELGVRLASILLVLPMSFAVYRTTMLLFGSAHAAASAAVFLNVTLMVAAGTVIVTPDAPLMAASSFVLLFLAELLVSGRGAWWLAVGAAVGAALLSKYTALFFGPVILIWLLTVPELRRWLRTPWPYLGGLVALAIFSPVIIWNAEHHWVSFAKQFGRARVDQFTLRYIVELIPTQIAFATPPVFALAAMGLFALSKRGEGTRAARVLINATVWTLTLYFVWHSLHGRVEANWFGPIYPALAIAAAVASDVARWQPCGRNFADACRRWALPVGVGMFVALVVQVNTGVLSAYRRDASVRSVGVGVRELAAGIEALRDKNGARCVLAQGYGVTSWLRFYLPEGTCVAQHFERIRWINAPPPDADQLAGRLLFVDDGSSNWPPVAHSFNQVEKIATLERKRGPLTIETFAINVVAEPKGDVLAPLPVELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQL3</accession>
    <name>Q3SQL3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2174</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315397.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2174"/>
    <dbReference type="EnsemblBacteria" id="ABA05428">
        <property type="protein sequence ID" value="ABA05428"/>
        <property type="gene ID" value="Nwi_2174"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2174"/>
    <dbReference type="eggNOG" id="ENOG5032TME">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1169944_0_0_5"/>
    <dbReference type="OMA" id="FRFPRQW"/>
    <dbReference type="OrthoDB" id="1135119at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="238" mass="26663" checksum="79B5B38C03D983B7" modified="2005-10-11" version="1">MALLLSAPRRSRGSRKSRAHHLTPLLAAAVIAMPSITAVAYLLWPTWTPKAPDDNKRLPVSIGQTVFNVPERAFRVKVQRHSGPQERIDLSFLYPSLATPDAPRRVTAESAEDEPRALDRIFLSIAAHHDSLAPDTLVRTIYPRYLDAARAQAEDGLSMHAFRDGTPYAGEDLFIAQSPALVTRCTREAMTPGMCLSERRIGDADLTFRFPRQWLTRWRDVAGAMERLAQQVRPGQDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQU2</accession>
    <name>Q3SQU2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Conjugal transfer protein TrbG/VirB9/CagX</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2092</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TrbG/VirB9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315327.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2092"/>
    <dbReference type="EnsemblBacteria" id="ABA05349">
        <property type="protein sequence ID" value="ABA05349"/>
        <property type="gene ID" value="Nwi_2092"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2092"/>
    <dbReference type="eggNOG" id="COG3504">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058585_1_0_5"/>
    <dbReference type="OMA" id="VSWEYPK"/>
    <dbReference type="OrthoDB" id="1251301at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06911">
        <property type="entry name" value="VirB9_CagX_TrbG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010258">
        <property type="entry name" value="Conjugal_tfr_TrbG/VirB9/CagX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014142">
        <property type="entry name" value="TrbG_Ti"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033645">
        <property type="entry name" value="VirB9/CagX/TrbG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038161">
        <property type="entry name" value="VirB9/CagX/TrbG_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03524">
        <property type="entry name" value="CagX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02775">
        <property type="entry name" value="TrbG_Ti"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229064" evidence="3">
        <location>
            <begin position="24"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="339" mass="36993" checksum="5BEC842C233AE0C4" modified="2005-10-11" version="1">MGDKRNNAVPALIAMMLTTTALAGCANKFIPPDINYDDAAPAVLSADPVGPVKVVELPKPLPLPGQLKPVNAAKTKPEPADPKKRVAQANEAARMQPVRNGFINAVQVYPFVVGALYQVYAAPGQVTDIALQPGEQLVGAGPVAAGDTVRWIVGDTLSGSGQTAQVHILVKPTRPDLQTNLVINTNLRTYHMELRSTEKTYMASVSWQYPQDQLIALRRQNQQAALTQPVASGVDISKLNFRYQIEGDDAPWRPLRAFDDGNKVYIEFPSGIGQGEMPPLFVIGPSGNSELVNYRARENYYVVDRLFAAAELRLGDKDSEKRVRIVRTDGRPGRGLRLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR24</accession>
    <name>Q3SR24_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dihydroorotase</fullName>
            <ecNumber evidence="2">3.5.2.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.2.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315253.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2008"/>
    <dbReference type="EnsemblBacteria" id="ABA05267">
        <property type="protein sequence ID" value="ABA05267"/>
        <property type="gene ID" value="Nwi_2008"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2008"/>
    <dbReference type="eggNOG" id="COG0044">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015572_1_0_5"/>
    <dbReference type="OMA" id="QHAQEPR"/>
    <dbReference type="OrthoDB" id="1319925at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004151">
        <property type="term" value="F:dihydroorotase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01317">
        <property type="entry name" value="DHOase_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004722">
        <property type="entry name" value="DHOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="1">
        <location>
            <begin position="60"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="433" mass="45597" checksum="6EBCB026645B6327" modified="2005-10-11" version="1">MLSGHRPILLANARIVDPARDLDGPGDVLIADGVIRDARHGIGAAGAPEGTDIINCAGMIVTPGLIDIRAFVGEPGAGHRETFASASCAAAAGGITTIVCQPDTSPVIDNSATVDFVLRRARDTAIVNIHPMAALTKGLAGKEMTEFGLLKEAGAVAFTDGARSVMNAQVMRRALTYARDFDALIVHHTEDAHLVGDGVMNEGELSSRLGLTGVPATAEAVMLERDMRLVGLTGGRYHAASLTCAESLDILMRARDAGLPVSASASINHLTLNENDIGPYRTFLKLSPPLRGEDDRRALVAALASGLIDVVMSDHNPQDVETKRLPFAEAAAGAIGLETMLTAALRLVHNAELDFKTLIRAMSTRPAELLGLPGGSLRPGAPADVIVIDPDVPWILDPADLKSQCKNTPFDESRFSGRVVRTIVGGRTVFEHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRG1</accession>
    <name>Q3SRG1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315126.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1870"/>
    <dbReference type="EnsemblBacteria" id="ABA05130">
        <property type="protein sequence ID" value="ABA05130"/>
        <property type="gene ID" value="Nwi_1870"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1870"/>
    <dbReference type="eggNOG" id="COG5454">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_160698_3_0_5"/>
    <dbReference type="OMA" id="VYFIIWW"/>
    <dbReference type="OrthoDB" id="2038848at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009935">
        <property type="entry name" value="DUF1467"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07330">
        <property type="entry name" value="DUF1467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="91" mass="10028" checksum="2E2A04445754AA56" modified="2005-10-11" version="1">MASKIFSGLAVYFIFWWITLFLMLPFGVRSQHEDGELIAGTDPGAPVKALMRRKVIWTTIVSSVIYGVAALAYYSGLLNVERLSRLMGASG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRH8</accession>
    <name>DXR_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">1-deoxy-D-xylulose 5-phosphate reductoisomerase</fullName>
            <shortName evidence="1">DXP reductoisomerase</shortName>
            <ecNumber evidence="1">1.1.1.267</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">1-deoxyxylulose-5-phosphate reductoisomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">2-C-methyl-D-erythritol 4-phosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dxr</name>
        <name type="ordered locus">Nwi_1853</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-C-methyl-D-erythritol 4-phosphate + NADP(+) = 1-deoxy-D-xylulose 5-phosphate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:13717"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
            <dbReference type="ChEBI" id="CHEBI:58262"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.267"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 1/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DXR family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.267" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315109.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRH8"/>
    <dbReference type="SMR" id="Q3SRH8"/>
    <dbReference type="STRING" id="323098.Nwi_1853"/>
    <dbReference type="EnsemblBacteria" id="ABA05113">
        <property type="protein sequence ID" value="ABA05113"/>
        <property type="gene ID" value="Nwi_1853"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1853"/>
    <dbReference type="eggNOG" id="COG0743">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035714_4_0_5"/>
    <dbReference type="OMA" id="PIDSEHF"/>
    <dbReference type="OrthoDB" id="1200722at2"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00092"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030604">
        <property type="term" value="F:1-deoxy-D-xylulose-5-phosphate reductoisomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00183">
        <property type="entry name" value="DXP_reductoisom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003821">
        <property type="entry name" value="DXP_reductoisomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013644">
        <property type="entry name" value="DXP_reductoisomerase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013512">
        <property type="entry name" value="DXP_reductoisomerase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026877">
        <property type="entry name" value="DXPR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036169">
        <property type="entry name" value="DXPR_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30525">
        <property type="entry name" value="PTHR30525"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08436">
        <property type="entry name" value="DXP_redisom_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02670">
        <property type="entry name" value="DXP_reductoisom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13288">
        <property type="entry name" value="DXPR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006205">
        <property type="entry name" value="Dxp_reductismrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69055">
        <property type="entry name" value="SSF69055"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00243">
        <property type="entry name" value="Dxr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000020284" description="1-deoxy-D-xylulose 5-phosphate reductoisomerase">
        <location>
            <begin position="1"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="22"/>
            <end position="51"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00183"/>
        </source>
    </evidence>
    <sequence length="407" mass="42750" checksum="B693CF5E980D7527" modified="2005-10-11" version="1">MSAVPLKNDKPVEAGVRTVTVLGATGSIGDSTMDLLRGARGRYRVEALTANSNVEGLVKLAREFDARFVALADTSYFDELKNALAGTGIEYGVGESAIIEAASRPADWLMAAVSGAAGLKPALAAVDRGATIALANKECLVCAGDFFMQRAARAGACVLPADSEHNALFQALSSGNRDELTRVIITASGGPFRTWAAADIENATLAQALKHPNWSMGRKITIDSASMMNKGLEVIEAACLFALEPEEIDVLVHPQSIVHGMVEFSDRSVVAQLGAPDMRTPIAHCLGWPERIAGPAAKLDLVSIGQLTFEAPDFARFPALRLAYDALRTGQGATTVYNAANEIAVAAFIAEKIRFGAIARLVEATLNGWVRAGNLAPLASADDAIAVDHNARNMAASLLPQIAAKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRJ5</accession>
    <name>Q3SRJ5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Parvulin-like PPIase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Peptidyl-prolyl cis-trans isomerase plp</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Rotamase plp</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_1836</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PpiC/parvulin rotamase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315092.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1836"/>
    <dbReference type="EnsemblBacteria" id="ABA05096">
        <property type="protein sequence ID" value="ABA05096"/>
        <property type="gene ID" value="Nwi_1836"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1836"/>
    <dbReference type="eggNOG" id="COG0760">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023843_2_1_5"/>
    <dbReference type="OMA" id="DNSQGWI"/>
    <dbReference type="OrthoDB" id="1201303at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000297">
        <property type="entry name" value="PPIase_PpiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027304">
        <property type="entry name" value="Trigger_fact/SurA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13145">
        <property type="entry name" value="Rotamase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109998">
        <property type="entry name" value="SSF109998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50198">
        <property type="entry name" value="PPIC_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0697">Rotamase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="PpiC" evidence="9">
        <location>
            <begin position="270"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA05096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="636" mass="69142" checksum="FEBD8A04E6C84B5E" modified="2005-10-11" version="1">MLRGIRNASSNWLGKTLMTIVMGLLIVAFGIWGIADIFHGFGQSALATVGGTEISTEQFRQIYNDRLQQIGRQFGRPLPPDQVRALGLDRQILQQVIAEAALDEDAQRKGLKVSDEEIGRQIKSDPNFKGANNAFDAARFAGLIRQLGYTEQRYVAEQRNMALRRQIVGTMTAGVEPSKTLLEALNRFQNEQRTIDYVKLGAAQAGTIDPPTADQLSGYFDAHKVQFRAPEYRKVAFVVLTPEEVAKWTAVSDDDARKIYEERKDKLSTPERRQLRQIMFPSVTDAQAAREKIAGGASFDDIARERGLATSDIDLGTISKAEIIDPAIANAAFSINSDEVSQPTQGRFGVALIKVGRIEPGNQPTYESVAEKLKRDVALDRARAEINNLHNKMEDERGGGASVVEAAKKVGLSAVTIEATDRSGRAPDGKPVSGIPQGVDLIAQAFASDVGVDNDTLQLGNNGFIWFDVIGVTPSRDRTLDEVKDKVEARWREDQISSRLMTKATDTIRKLEQGGKLADEAASLGLKVESASAIKRDATTTALPAAVVAAVCRAAKDAAGQAPGAGNDEWFIFRVTDVKVPTLDLASDETKKLREGVQRGLSDEYIGQYIVKLEAEIGTKINQAAVAQITGASSNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRK2</accession>
    <name>Q3SRK2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1829</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315085.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1829"/>
    <dbReference type="EnsemblBacteria" id="ABA05089">
        <property type="protein sequence ID" value="ABA05089"/>
        <property type="gene ID" value="Nwi_1829"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1829"/>
    <dbReference type="eggNOG" id="ENOG50346ME">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1164900_0_0_5"/>
    <dbReference type="OMA" id="INPYRID"/>
    <dbReference type="OrthoDB" id="2115974at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229226" evidence="1">
        <location>
            <begin position="24"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="238" mass="24113" checksum="D6B528B042902ED9" modified="2005-10-11" version="1">MMLRGLLGLVTLVTIASPLSALAAEPGFFAGLDVSGGAAFGSSSTVNGGAPFAGGGVVGNVKLGNFVGGGGHVGYRFDSAMSAFVSYQHGQGGIGWDANFPLYGASSRFDGTAISNAILGNVAYEIPLTDVISMRTTAGLGITFNTLSRVIETNKATGIFLADLATHTRTGTAAQVGLGFRYKIAPSAEVGLDGLVAYAGGFETGSARSGNLGITSINPYRIDDVWRASLSASLTIGF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRS3</accession>
    <name>LFTR_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Leucyl/phenylalanyl-tRNA--protein transferase</fullName>
            <ecNumber evidence="1">2.3.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">L/F-transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Leucyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phenyalanyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aat</name>
        <name type="ordered locus">Nwi_1757</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Functions in the N-end rule pathway of protein degradation where it conjugates Leu, Phe and, less efficiently, Met from aminoacyl-tRNAs to the N-termini of proteins containing an N-terminal arginine or lysine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-leucyl-tRNA(Leu) + N-terminal L-lysyl-[protein] = H(+) + N-terminal L-leucyl-L-lysyl-[protein] + tRNA(Leu)</text>
            <dbReference type="Rhea" id="RHEA:12340"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="Rhea" id="RHEA-COMP:12670"/>
            <dbReference type="Rhea" id="RHEA-COMP:12671"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:65249"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:133043"/>
            <dbReference type="EC" id="2.3.2.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-leucyl-tRNA(Leu) + N-terminal L-arginyl-[protein] = H(+) + N-terminal L-leucyl-L-arginyl-[protein] + tRNA(Leu)</text>
            <dbReference type="Rhea" id="RHEA:50416"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="Rhea" id="RHEA-COMP:12672"/>
            <dbReference type="Rhea" id="RHEA-COMP:12673"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:64719"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:133044"/>
            <dbReference type="EC" id="2.3.2.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an N-terminal L-alpha-aminoacyl-[protein] + L-phenylalanyl-tRNA(Phe) = an N-terminal L-phenylalanyl-L-alpha-aminoacyl-[protein] + tRNA(Phe)</text>
            <dbReference type="Rhea" id="RHEA:43632"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="Rhea" id="RHEA-COMP:10636"/>
            <dbReference type="Rhea" id="RHEA-COMP:10637"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:78597"/>
            <dbReference type="ChEBI" id="CHEBI:83561"/>
            <dbReference type="EC" id="2.3.2.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the L/F-transferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.6" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315014.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRS3"/>
    <dbReference type="SMR" id="Q3SRS3"/>
    <dbReference type="STRING" id="323098.Nwi_1757"/>
    <dbReference type="EnsemblBacteria" id="ABA05018">
        <property type="protein sequence ID" value="ABA05018"/>
        <property type="gene ID" value="Nwi_1757"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1757"/>
    <dbReference type="eggNOG" id="COG2360">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075045_1_1_5"/>
    <dbReference type="OMA" id="YRQGIFP"/>
    <dbReference type="OrthoDB" id="1670846at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008914">
        <property type="term" value="F:leucyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3550">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00688">
        <property type="entry name" value="Leu_Phe_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004616">
        <property type="entry name" value="Leu/Phe-tRNA_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042203">
        <property type="entry name" value="Leu/Phe-tRNA_Trfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042221">
        <property type="entry name" value="Leu/Phe-tRNA_Trfase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30098">
        <property type="entry name" value="PTHR30098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03588">
        <property type="entry name" value="Leu_Phe_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00667">
        <property type="entry name" value="aat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000258069" description="Leucyl/phenylalanyl-tRNA--protein transferase">
        <location>
            <begin position="1"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00688"/>
        </source>
    </evidence>
    <sequence length="225" mass="24882" checksum="C09E15544BF646EA" modified="2005-10-11" version="1">MSRETASSEITPELLLRAYACGIFPMAESVDDPTLFWVEPKMRGVVPLESFRIPSRLARTVRSDSFTVTVDTAFDAVINGCAAPQPGRNNTWINHRIRELYIGLHELGHCHSVEVWRDNELAGGLYGVRLGRAFFGESMFHVVRDASKVALVHLVARLIAGGFVLLDTQFVTDHLRGFGAIEIPRRRYRALLDVALEGRADFGALPLDRPVPGAEALKAIADRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRT5</accession>
    <name>Q3SRT5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Pyruvate ferredoxin/flavodoxin oxidoreductase</fullName>
            <ecNumber evidence="9">1.2.7.8</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_1745</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pyruvate:ferredoxin/flavodoxin oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.8" evidence="9"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315002.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1745"/>
    <dbReference type="EnsemblBacteria" id="ABA05006">
        <property type="protein sequence ID" value="ABA05006"/>
        <property type="gene ID" value="Nwi_1745"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1745"/>
    <dbReference type="eggNOG" id="COG1014">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4231">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009166_1_0_5"/>
    <dbReference type="OMA" id="PRVVGKF"/>
    <dbReference type="OrthoDB" id="37279at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043805">
        <property type="term" value="F:indolepyruvate ferredoxin oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="SSF53323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="630"/>
            <end position="662"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1165" mass="127649" checksum="09502C8DB60153E8" modified="2005-10-11" version="1">MGINQGPISLDQKYTQGSGHVFMTGLQALVRLPMAQIRRDRAAGLNTAGFISGYRGSPLGGYDQQLFAARHHLDRHGIKFQPGVNEDLAATAVWGSQQVNLSPGARYDGVVGLWYGKGPGVDRCGDVFRHGNAAGSARNGGVLCLAGDDHGAKSSTVPHQSDHAFIAALMPYLYPSSIHEMIEMGLLGIAMSRYSGCWVGMKVITETVETTAEIDLTDEMKPFVIPADFEMPPGGLNLRWPDDRYDQDLRLQDHKGFAAVAFARANNINRVTMDSPNARFGIVASGKSYEDIRQALRELGITEQVAAKIGLRLLKIGMPWPLEPQSIREFAVGLEEIFIVEERREIVENQIKRELFNWRDDVRPRIIGKMDDHDKRFLPFAQELGVASLASYLTERLLRLNLNPEIAEMLRAKADWFNGRQASQMQPATPVMRVPYFCSGCPHNTSTKVPDGSRAMAGIGCHFMALWMDRNTETYTHMGGEGAPWVGIAPFTNEKHIFANLGDGTYFHSGSLAIRQAVASGANITYKILYNDAVAMTGGQHFDGELSPQRITFQLHAEGIRDIYLVSESPDSYPASDIAPGTRTAHRDDLDAVMKTCRETKGTSAIVFVQTCAAEKRRRRKRGLMEDPQRRVLINPEVCEGCGDCSVQSNCISVEPLETEMGRKRSINQSSCNKDYSCLKGFCPSFVTIDGGRLRKRAPADLGEIGDLPEPASRPSLDRPYNIGVGGVGGTGVLTIGALLGMAAHIEGKASMILDMSGLAQKGGAVLSHVRLSRNPAEVICSRIVTGTADLVIAADEVVAAAKETITLCESSRTYGLINTHLIPTAGFVLDRDFNFQSGKVGRVLETALRKDSTFFNFTRPAETLLGDSIATNMMMMGYAYQKGLLPLTSEAIEQAIEMNGVAVKMNVGAFRLGRLAVVDPARLVEMMKGTGEAAPPKALGDMSLDEIVVHRAAHLADYQNARLADKYRAMVSRIRQAANEGGYGDALPRAVAINYAKLLAYKDEYEVARLYTDGRFRKQVSDQFEGDYSIRFHLAPPLFDGRKDALGRPAKKPYGKSLMMPVFRLLAKMKGLRGTAFDVFGYNPERRLERSLIAEYETDVAAVLDRLSPANVEAAIELLSLPDRIRGYGPVKEKAVADTRGRHAELLRDLANPPPVAPRQMAAE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-04-08" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRU5</accession>
    <name>ACYP_NITWN</name>
    <protein>
        <recommendedName>
            <fullName>Acylphosphatase</fullName>
            <ecNumber>3.6.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Acylphosphate phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">acyP</name>
        <name type="ordered locus">Nwi_1735</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>an acyl phosphate + H2O = a carboxylate + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="EC" id="3.6.1.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acylphosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314992.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRU5"/>
    <dbReference type="SMR" id="Q3SRU5"/>
    <dbReference type="STRING" id="323098.Nwi_1735"/>
    <dbReference type="EnsemblBacteria" id="ABA04996">
        <property type="protein sequence ID" value="ABA04996"/>
        <property type="gene ID" value="Nwi_1735"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1735"/>
    <dbReference type="eggNOG" id="COG1254">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141932_3_2_5"/>
    <dbReference type="OMA" id="VGFRWSM"/>
    <dbReference type="OrthoDB" id="2034659at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003998">
        <property type="term" value="F:acylphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020456">
        <property type="entry name" value="Acylphosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001792">
        <property type="entry name" value="Acylphosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036046">
        <property type="entry name" value="Acylphosphatase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017968">
        <property type="entry name" value="Acylphosphatase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47268">
        <property type="entry name" value="PTHR47268"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00708">
        <property type="entry name" value="Acylphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54975">
        <property type="entry name" value="SSF54975"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00150">
        <property type="entry name" value="ACYLPHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00151">
        <property type="entry name" value="ACYLPHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51160">
        <property type="entry name" value="ACYLPHOSPHATASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000326758" description="Acylphosphatase">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Acylphosphatase-like" evidence="1">
        <location>
            <begin position="5"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="20"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="99" mass="11181" checksum="CDFFA7A5EE637E3D" modified="2005-10-11" version="1">MHEVVRQVMIRGRVQGVGFRYWTMREAIRFGVGGWVRNRRDGSVEALFAGSTEAVAEMITRCRSGPEFARVDDIEDQPVAANALKMIRPGERFSQLPTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRY1</accession>
    <name>Q3SRY1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Peptidase S11, D-alanyl-D-alanine carboxypeptidase 1</fullName>
            <ecNumber evidence="12">3.4.16.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_1699</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the peptidase S11 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.4" evidence="12"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314958.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1699"/>
    <dbReference type="EnsemblBacteria" id="ABA04960">
        <property type="protein sequence ID" value="ABA04960"/>
        <property type="gene ID" value="Nwi_1699"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1699"/>
    <dbReference type="eggNOG" id="COG1686">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027070_1_1_5"/>
    <dbReference type="OMA" id="PRYYRYF"/>
    <dbReference type="OrthoDB" id="1499212at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042834">
        <property type="term" value="F:peptidoglycan binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009002">
        <property type="term" value="F:serine-type D-Ala-D-Ala carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018044">
        <property type="entry name" value="Peptidase_S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001967">
        <property type="entry name" value="Peptidase_S11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007730">
        <property type="entry name" value="SPOR-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036680">
        <property type="entry name" value="SPOR-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00768">
        <property type="entry name" value="Peptidase_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05036">
        <property type="entry name" value="SPOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00725">
        <property type="entry name" value="DADACBPTASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="3" id="KW-0133">Cell shape</keyword>
    <keyword evidence="5" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="12" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Peptidase_S11" evidence="10">
        <location>
            <begin position="58"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="SPOR" evidence="11">
        <location>
            <begin position="507"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="313"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="420"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="6">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR618044-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR618044-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA04960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="587" mass="62378" checksum="C5752ED4489A95B5" modified="2005-10-11" version="1">MLRTTLASSRATRVGALGLAAIIAVVFTIDHADARRARQHHRHHQQQVRHSYNPPFASIVVDANSGATLSSTSPDSLRHPASLTKMMTLYMLFERLDAGRIGLDSEMRVSARAAAQNPTKLGLRPGQTIRVEDAIKGLVTKSANDAAVVIAEAIAGDEDDFARMMTRKARALGMTRTVYRNASGLPDDDQVTTARDQATLGRALQDRFPRYYRYFATTSFTFRGRTIRGHNRLVGSVDGVDGIKTGYIRASGFNLVTNLKRGNRHLVGVVLGGRSGGSRDATMRKLLAENLDKAATRRTVAAIAESSMAEANARAAEDQARSRPRGTTQVDGAVQVASTVPELVSLPAERPAHLSRSLTAAPAAALPPKRPLAEPAPLTSGVIETQPIDAVPGSAEPMKPVRVKTVQVRAKAVRLASAGPAPALSPAAGDAAADREAAPETSGSVVARANMPPQPANFGTGQGILGVLPASSLPASTRQAMVHADPAPAVQPRTVQKNGALKPVASHSGWIIQVGALETESQARERLDAARGRAPRMLGKADPFTEPVAKGSKKLYRARFAGLERDEAEAVCRKLKRSDISCITIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS17</accession>
    <name>Q3SS17_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">FeS assembly ATPase SufC</fullName>
            <ecNumber evidence="7">3.6.3.30</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_1663</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in beta-(1--&gt;2)glucan export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. Ycf16 family.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.30" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314923.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1663"/>
    <dbReference type="EnsemblBacteria" id="ABA04924">
        <property type="protein sequence ID" value="ABA04924"/>
        <property type="gene ID" value="Nwi_1663"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1663"/>
    <dbReference type="eggNOG" id="COG0396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_48_1_5"/>
    <dbReference type="OMA" id="MAMLEPK"/>
    <dbReference type="OrthoDB" id="1214676at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03217">
        <property type="entry name" value="ABC_FeS_Assembly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010230">
        <property type="entry name" value="FeS-cluster_ATPase_SufC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43204">
        <property type="entry name" value="PTHR43204"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01978">
        <property type="entry name" value="sufC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="4"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="249" mass="27291" checksum="A2E1FEE56A7F9C66" modified="2005-10-11" version="1">MPLLEVKDLQVRVEEREILHGLSLTVNKGEVHAIMGPNGSGKSTLSHVIAGKPGYEVTGGQILFKGEDLLEMEPDERAAKGVFLAFQYPVEIPGVATMTFLRTALNAQRKARGESEYSTPNFLKKVRDVAASLNIPQDMLRRGINVGFSGGEKKRNEVLQMALFEPSLCILDEMDSGLDIDALRVAADGVNALRSPDRAMVVITHYQRLLNYIVPDVVHVMSKGRVVKSGNKDLALELEASGYAQFEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS22</accession>
    <name>Q3SS22_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Diguanylate cyclase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1658</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1658"/>
    <dbReference type="EnsemblBacteria" id="ABA04919">
        <property type="protein sequence ID" value="ABA04919"/>
        <property type="gene ID" value="Nwi_1658"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1658"/>
    <dbReference type="eggNOG" id="COG3706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_11_5_5"/>
    <dbReference type="OMA" id="YAVWYEY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGDEF" evidence="2">
        <location>
            <begin position="207"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="149"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="354" mass="38916" checksum="794599E0493D6654" modified="2005-10-11" version="1">MVELNEHERTMAFAEVALGQIRSLRQAAVPRNYEVWYVYAAGYNCSLNRTINETLARNGTLSDVDLEQIYDTYLSPIRTTERIDKVGARVINEIDEVILLITGARSMAAGFGDSLREAARQLGAAGSHAQIKSVIAALMTSTLDMQEANKVLEDRLELSKVEINNLQHSLEAIRAESMTDPLTGLGNRKYFDRAVADAVALAMSEGEPLSLLMFDIDHFKSFNDNYGHLTGDQVLRLVAMSLKQNIKGQDITARYGGEEFAVVLPSTALKQALTVADHIRRAVMAKELKKKSTGEILGRVTISIGVSMLRPGDTPDSLMERADACLYAAKRGGRNRAICETDPEYFAGAETRVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS76</accession>
    <name>Q3SS76_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Carbamoyl-phosphate synthetase large chain</fullName>
            <ecNumber evidence="9">6.3.4.14</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_1604</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="6.3.4.14" evidence="9"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314869.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1604"/>
    <dbReference type="EnsemblBacteria" id="ABA04865">
        <property type="protein sequence ID" value="ABA04865"/>
        <property type="gene ID" value="Nwi_1604"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1604"/>
    <dbReference type="eggNOG" id="COG4770">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000395_3_1_5"/>
    <dbReference type="OMA" id="KITTWRT"/>
    <dbReference type="OrthoDB" id="361205at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004075">
        <property type="term" value="F:biotin carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CbamoylP_synth_lsu-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="SSF51246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="8">
        <location>
            <begin position="9"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="7">
        <location>
            <begin position="128"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="6">
        <location>
            <begin position="589"/>
            <end position="665"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA04865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="668" mass="72600" checksum="947960F21F36AD4A" modified="2005-10-11" version="1">MAGAPTCRPFTTLLIANRGEIACRVIRTARAMCLRTVAVYSEADRDALHVALADEAVCLGPAPARDSYLNVGKVIDAARRTGAEGVHPGYGFLSENAGFAQACADAGLVFVGPTAAMMTAMGTKSDARALMQKADVPLVPGYHGEAQDEATLRNTADRIGYPVLVKASGGGGGRGMRIVRSAAELAPAIVSARREAGAAFGDERMLIEKYVENPRHIEVQVIGDSHGNLLSLFERECTLQRRHQKVIEEAPSPTLNAAQRDRVCEAARKAAAIVNYIGAGTVEFVSDGRDVFFIEMNTRLQVEHPVTELVTGIDLVEWQLRVAFGEKLPLTQDQITLNGHAIEARVYAEDPDRNFMPSVGRIRMWRTPPEVDGLRIDAGYREGDAVPPSYDAMLAKVIAWAPTRQAAIRRLARGLDETDVRGIATNIPFLSALITHADVRANAVDTGFIERHLQELTRPSTAPGETELAAAVAAVLLEESKASSTDPASPWRTAGWMAVGRRRQRFVFRQGQGQAEHEVTFVYGNGPDEIQLADKKLPFAFAADDSEGMILTLDGRRSHVVFAVDGRELYLRTRNGRFELHWVDPFGEDEEEQAGEDRIIAPLPGTVVALFAEEGTLLEKGAPILALEVMKMEQTLRAPFAGRLKKIKCRVGDVVREGVELAEIEAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS78</accession>
    <name>Q3SS78_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1602</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1602"/>
    <dbReference type="EnsemblBacteria" id="ABA04863">
        <property type="protein sequence ID" value="ABA04863"/>
        <property type="gene ID" value="Nwi_1602"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1602"/>
    <dbReference type="HOGENOM" id="CLU_2047227_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="120" mass="12375" checksum="FA54557FD256F621" modified="2005-10-11" version="1">MPANAAVGANVAVPSDAAAIAANAILRNIELSPHDGDAEASAVNISSGWSDVAPGEQVQCVGGYGMDSNRSVMQITELVIARRIAEGGTVRCPMRLPSLTGADISAQFLRPLNQGRHHGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSA0</accession>
    <name>Q3SSA0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1580</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04841">
        <property type="protein sequence ID" value="ABA04841"/>
        <property type="gene ID" value="Nwi_1580"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1580"/>
    <dbReference type="HOGENOM" id="CLU_2539146_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="83" mass="9617" checksum="629E008162BD2E85" modified="2005-10-11" version="1">MTIIVRRMTALRRFASDISAFRFKFRTRQAFSMTKRKAEAGFSRFRKLGTLTQYGIPGGKTAMHFDALCQVEMITCDMPPRFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSC5</accession>
    <name>Q3SSC5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1555</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314822.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1555"/>
    <dbReference type="EnsemblBacteria" id="ABA04816">
        <property type="protein sequence ID" value="ABA04816"/>
        <property type="gene ID" value="Nwi_1555"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1555"/>
    <dbReference type="eggNOG" id="ENOG5030XZ6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1632206_0_0_5"/>
    <dbReference type="OMA" id="VRMHREI"/>
    <dbReference type="OrthoDB" id="1810735at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044925">
        <property type="entry name" value="His-Me_finger_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54060">
        <property type="entry name" value="SSF54060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="177" mass="20145" checksum="97436C927C6AB45F" modified="2005-10-11" version="1">MSGYDKTLADKLDAIAPIVTHLLKPQPVELITLEQVDLSRTAWRLIPLSERRGVYCLVDADDYGWLTEVTWNISWGSRTPWQQYAKRNVGSGRATVRMHREILIRHDPRSDRFMAKRHVDHVNGQTLDNRRCNLRWLTPGENAANRRPRGRVPSLESIIAELLTAHAAGGAMAEVPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSE0</accession>
    <name>Q3SSE0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase_M15_4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1540</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314807.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1540"/>
    <dbReference type="EnsemblBacteria" id="ABA04801">
        <property type="protein sequence ID" value="ABA04801"/>
        <property type="gene ID" value="Nwi_1540"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1540"/>
    <dbReference type="eggNOG" id="COG0741">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1085167_0_0_5"/>
    <dbReference type="OMA" id="IWEHYGR"/>
    <dbReference type="OrthoDB" id="1915613at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009045">
        <property type="entry name" value="Hedgehog_sig/DD-Pept_Zn-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039561">
        <property type="entry name" value="Peptidase_M15C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13539">
        <property type="entry name" value="Peptidase_M15_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55166">
        <property type="entry name" value="SSF55166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M15_4" evidence="2">
        <location>
            <begin position="92"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="257" mass="28697" checksum="48777872301442B5" modified="2005-10-11" version="1">MTKWPRDNQADLLAFYGTPGRDVERQLVDVVPPFQMYYDGKPISRIRFHRKAAGALRAALDEIWEHYGRDQRRIDALGISKYAGAYNPRKVRGSATRWSNHAYGAAIDLNAEQNGLGAGRGTMPQPVINAFKRQGARWGGDYRGRTDPMHFEFCDASGYPGVTPVKLFDAPEIDADSDAHIEHSAAIDVSPKPTWLRRKWKAVTGWFSGGAGLGFFGYLTDWRVIAVLLGAMVLVAVLFVLFMGPSNVRAWIKRQVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSE2</accession>
    <name>Q3SSE2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1538</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314805.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1538"/>
    <dbReference type="EnsemblBacteria" id="ABA04799">
        <property type="protein sequence ID" value="ABA04799"/>
        <property type="gene ID" value="Nwi_1538"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1538"/>
    <dbReference type="eggNOG" id="ENOG5033IHQ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_192019_0_0_5"/>
    <dbReference type="OMA" id="ISKIASW"/>
    <dbReference type="OrthoDB" id="2070288at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="77" mass="8556" checksum="80C73DC5CA5C36FA" modified="2005-10-11" version="1">MSEAKRFDDLPPATKEFLTNLRPDEIKTLNDGIRLINSALTVGRFMKWVIITMLGILAGIVMFGESISKIASWMKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSQ5</accession>
    <name>Q3SSQ5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1425</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314695.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1425"/>
    <dbReference type="EnsemblBacteria" id="ABA04686">
        <property type="protein sequence ID" value="ABA04686"/>
        <property type="gene ID" value="Nwi_1425"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1425"/>
    <dbReference type="eggNOG" id="COG3146">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036032_1_1_5"/>
    <dbReference type="OMA" id="HFEVCYY"/>
    <dbReference type="OrthoDB" id="891566at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007434">
        <property type="entry name" value="FemAB-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04339">
        <property type="entry name" value="FemAB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="414" mass="45848" checksum="E8CD89F5F48899D1" modified="2005-10-11" version="1">MIPPSEISLEAVGSVSEIPAADWDACANPASGPGGGSDSAVPAPLPDSSQSSKCRYNPFVSHAFFIALESSNSACSRTGWGPRHLIARQDGSIVGIVPCYLKSHSQGEYVFDHGWANAYESAGGRYYPKLLVSVPFTPATGPRLLIRPGNDEADVRAALAGGLVGLCDATRASSVHVTFARKDEWAFLAEKGFLRRTDQQFHWRNDGYSCFEDFLASLNSRHRKAIRRERRDALANGITIHVLTGGDLTEEIWDAFFQFYMETGTRKWGRPYLTRSFYSLIGQSMSRDIALIMARRDGRWIAGAINFIGSDTLFGRHWGAIEHHPFLHFEVCYYQAIDFAIQHRLRTVEAGAQGEHKIARGYLPQTTYSAHYIADPGLRRAVGDFLNRERHYVAEAARELTEAGPFRKTGDGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSQ7</accession>
    <name>Q3SSQ7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Lytic transglycosylase, catalytic</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_1423</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the virb1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314693.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1423"/>
    <dbReference type="CAZy" id="GH23">
        <property type="family name" value="Glycoside Hydrolase Family 23"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04684">
        <property type="protein sequence ID" value="ABA04684"/>
        <property type="gene ID" value="Nwi_1423"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1423"/>
    <dbReference type="eggNOG" id="COG0741">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070265_0_0_5"/>
    <dbReference type="OMA" id="ICLMIES"/>
    <dbReference type="OrthoDB" id="1444566at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042834">
        <property type="term" value="F:peptidoglycan binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007730">
        <property type="entry name" value="SPOR-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008258">
        <property type="entry name" value="Transglycosylase_SLT_dom_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01464">
        <property type="entry name" value="SLT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05036">
        <property type="entry name" value="SPOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SLT" evidence="3">
        <location>
            <begin position="123"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="SPOR" evidence="4">
        <location>
            <begin position="291"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="78"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="84"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="380" mass="41218" checksum="D0BDF158DE64C03E" modified="2005-10-11" version="1">MHPASLPSRKRTIAFSSEAESGSRQENASKHKDKVFHHFHKTVKDSGIAQRLSGRIRCALAAFLIVVVPGPASAQVGAETAAAPEHAKPDKTDKPDKAVEPDKVDKSDKVDLDTREAICLMVESAARAESLPLEFFARVIWQESRFQPDAVGPMTRSGQHAQGIAQFMPGTANERRLLDPFDPVQALPKSAEYLNELRGRFGNLGLAAAAYNAGPRRLQDWLDGKGSMPAETRNYVVAITGSPIEDWAAAGKSGKPPAPSDTTSCRELMALLKRAPNPFVTGLEQHVKLGADKPWGVQLAAGFNRAGAMAMYARAIRHLGAVIGDRDPSLLGSLWRSRGTRTFYQVRIGADSRMEADDLCNRIRRAGGACLVLRNPRVKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSU2</accession>
    <name>RPOA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit alpha</fullName>
            <shortName evidence="1">RNAP subunit alpha</shortName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">RNA polymerase subunit alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Transcriptase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpoA</name>
        <name type="ordered locus">Nwi_1388</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA polymerase alpha chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSU2"/>
    <dbReference type="SMR" id="Q3SSU2"/>
    <dbReference type="STRING" id="323098.Nwi_1388"/>
    <dbReference type="EnsemblBacteria" id="ABA04649">
        <property type="protein sequence ID" value="ABA04649"/>
        <property type="gene ID" value="Nwi_1388"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1388"/>
    <dbReference type="eggNOG" id="COG0202">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053084_0_0_5"/>
    <dbReference type="OMA" id="LMKFRNF"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00059">
        <property type="entry name" value="RNApol_bact_RpoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011262">
        <property type="entry name" value="DNA-dir_RNA_pol_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011263">
        <property type="entry name" value="DNA-dir_RNA_pol_RpoA/D/Rpb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011773">
        <property type="entry name" value="DNA-dir_RpoA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011260">
        <property type="entry name" value="RNAP_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036643">
        <property type="entry name" value="RNApol_insert_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32108">
        <property type="entry name" value="PTHR32108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01000">
        <property type="entry name" value="RNA_pol_A_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03118">
        <property type="entry name" value="RNA_pol_A_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01193">
        <property type="entry name" value="RNA_pol_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00662">
        <property type="entry name" value="RPOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="SSF55257"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56553">
        <property type="entry name" value="SSF56553"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02027">
        <property type="entry name" value="rpoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000225284" description="DNA-directed RNA polymerase subunit alpha">
        <location>
            <begin position="1"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Alpha N-terminal domain (alpha-NTD)" evidence="1">
        <location>
            <begin position="1"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Alpha C-terminal domain (alpha-CTD)" evidence="1">
        <location>
            <begin position="251"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00059"/>
        </source>
    </evidence>
    <sequence length="339" mass="37517" checksum="3725F04A136A6620" modified="2005-10-11" version="1">MTIQKNWQELIRPNKLQVVPGSDPSRFATLIAEPLERGFGQTLGNALRRILLSSLQGAAVQSVHIDGVLHEFSSIAGVREDVTDIVLNIKDIAIRMQGEGPKRMVVKKQGPGTVTAGDIQTVGDVVVLNPDLQICTLDDGAEIRMEFTVASGKGYVAAERNRPEDAPIGLIPVDSLYSPVRKVSYKVENTREGQILDYDKLTMTIETNGGVTPEDAVAFAARILQDQLNVFVNFEEPRKEVTTEIIPDLAFNPAFLKKVDELELSVRSANCLKNDNIVYIGDLVQKSEAEMLRTPNFGRKSLNEIKEVLAQMGLHLGMEVPGWPPENIDELAKRFEDHY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-03" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSV0</accession>
    <name>RL18_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplR</name>
        <name type="ordered locus">Nwi_1380</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This is one of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit; part of the 5S rRNA/L5/L18/L25 subcomplex. Contacts the 5S and 23S rRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314654.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSV0"/>
    <dbReference type="SMR" id="Q3SSV0"/>
    <dbReference type="STRING" id="323098.Nwi_1380"/>
    <dbReference type="EnsemblBacteria" id="ABA04641">
        <property type="protein sequence ID" value="ABA04641"/>
        <property type="gene ID" value="Nwi_1380"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1380"/>
    <dbReference type="eggNOG" id="COG0256">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098841_0_1_5"/>
    <dbReference type="OMA" id="NKQIYAQ"/>
    <dbReference type="OrthoDB" id="1963491at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01337_B">
        <property type="entry name" value="Ribosomal_L18_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005484">
        <property type="entry name" value="Ribosomal_L18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004389">
        <property type="entry name" value="Ribosomal_L18_bac-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12899">
        <property type="entry name" value="PTHR12899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00861">
        <property type="entry name" value="Ribosomal_L18p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00060">
        <property type="entry name" value="L18_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000251335" description="50S ribosomal protein L18">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01337"/>
        </source>
    </evidence>
    <sequence length="120" mass="13098" checksum="44D9C9C9B8FEEBF8" modified="2005-10-11" version="1">MSKLKITNARRKQRVRLSLRRIANGRPRLSVFRSSKHIYAQVIDDLKGETLASASSLEKAMRDAGNTGADIDAAKAVGKLVAERAVKNGVKEVVFDRGGYLYHGRVKALADAARESGLSF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSW7</accession>
    <name>RS10_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsJ</name>
        <name type="ordered locus">Nwi_1363</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the binding of tRNA to the ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_002712302.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSW7"/>
    <dbReference type="SMR" id="Q3SSW7"/>
    <dbReference type="STRING" id="323098.Nwi_1363"/>
    <dbReference type="EnsemblBacteria" id="ABA04624">
        <property type="protein sequence ID" value="ABA04624"/>
        <property type="gene ID" value="Nwi_1363"/>
    </dbReference>
    <dbReference type="GeneID" id="66894337"/>
    <dbReference type="KEGG" id="nwi:Nwi_1363"/>
    <dbReference type="eggNOG" id="COG0051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122625_1_3_5"/>
    <dbReference type="OMA" id="QFEIRVH"/>
    <dbReference type="OrthoDB" id="2012682at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00508">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001848">
        <property type="entry name" value="Ribosomal_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018268">
        <property type="entry name" value="Ribosomal_S10_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027486">
        <property type="entry name" value="Ribosomal_S10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036838">
        <property type="entry name" value="Ribosomal_S10_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11700">
        <property type="entry name" value="PTHR11700"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00338">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00971">
        <property type="entry name" value="RIBOSOMALS10"/>
    </dbReference>
    <dbReference type="SMART" id="SM01403">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54999">
        <property type="entry name" value="SSF54999"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01049">
        <property type="entry name" value="rpsJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00361">
        <property type="entry name" value="RIBOSOMAL_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000237069" description="30S ribosomal protein S10">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00508"/>
        </source>
    </evidence>
    <sequence length="102" mass="11669" checksum="60709820CED359DA" modified="2005-10-11" version="1">MNGQNIRIRLKAFDHRILDTSTREIVNTAKRTGAQVRGPIPLPTRIEKFTVNRSPHVDKKSREQFEMRTHKRLLDIVDPTPQTVDALMKLDLAAGVDVEIKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST00</accession>
    <name>HIS3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribosyl-AMP cyclohydrolase</fullName>
            <shortName evidence="1">PRA-CH</shortName>
            <ecNumber evidence="1">3.5.4.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisI</name>
        <name type="ordered locus">Nwi_1330</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the hydrolysis of the adenine ring of phosphoribosyl-AMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(5-phospho-beta-D-ribosyl)-5'-AMP + H2O = 1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:20049"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="EC" id="3.5.4.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 3/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PRA-CH family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.19" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314609.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ST00"/>
    <dbReference type="SMR" id="Q3ST00"/>
    <dbReference type="STRING" id="323098.Nwi_1330"/>
    <dbReference type="EnsemblBacteria" id="ABA04591">
        <property type="protein sequence ID" value="ABA04591"/>
        <property type="gene ID" value="Nwi_1330"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1330"/>
    <dbReference type="eggNOG" id="COG0139">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048577_5_0_5"/>
    <dbReference type="OMA" id="TGYRSCF"/>
    <dbReference type="OrthoDB" id="1842189at2"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00008"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004635">
        <property type="term" value="F:phosphoribosyl-AMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01021">
        <property type="entry name" value="HisI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026660">
        <property type="entry name" value="PRA-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002496">
        <property type="entry name" value="PRib_AMP_CycHydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038019">
        <property type="entry name" value="PRib_AMP_CycHydrolase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01502">
        <property type="entry name" value="PRA-CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141734">
        <property type="entry name" value="SSF141734"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000229827" description="Phosphoribosyl-AMP cyclohydrolase">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01021"/>
        </source>
    </evidence>
    <sequence length="145" mass="15977" checksum="0A399F3F738A4752" modified="2005-10-11" version="1">MSGSKSSSETEEGLAFQPSFDASGLVTCVATDAKTGDVLMVAHMNEEALRRTVETGDAWYYSRSRKALWRKGESSGQVQRVLEMRTDCDQDAVWIKVEQQGAACHTGRRSCFYRAVAKGEGAGIRLAFVDAERLFDPAEVYRAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST08</accession>
    <name>Q3ST08_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1322</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1322"/>
    <dbReference type="EnsemblBacteria" id="ABA04583">
        <property type="protein sequence ID" value="ABA04583"/>
        <property type="gene ID" value="Nwi_1322"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1322"/>
    <dbReference type="eggNOG" id="COG3293">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055261_2_1_5"/>
    <dbReference type="OMA" id="WRIFEAM"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025161">
        <property type="entry name" value="Put_IS4/IS5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13340">
        <property type="entry name" value="DUF4096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="124" mass="14012" checksum="EF14809986B6F673" modified="2005-10-11" version="1">MAAKRYELNEAQWSKISSLLPGKTGDAGRTAADNRLFVNGCLWVLRSGAHWCDLPERYGKWKTVHRRFSRWCHAGVWERVFAALTADRDNQYLMIDSTIVRAHQQAASGKGGPGIRRWGAPEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST90</accession>
    <name>Q3ST90_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Rhodanese-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1239</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1239"/>
    <dbReference type="EnsemblBacteria" id="ABA04501">
        <property type="protein sequence ID" value="ABA04501"/>
        <property type="gene ID" value="Nwi_1239"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1239"/>
    <dbReference type="eggNOG" id="COG0607">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089574_6_3_5"/>
    <dbReference type="OMA" id="GGMLGWK"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="SSF52821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="47"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="137" mass="14426" checksum="A47F7705071F41E0" modified="2005-10-11" version="1">MRGRRSGDTGIAPGASVRRQKREEVTLANLNFVVHDLTPEDVSKGITDGRYLLVDVREPNEIAAEAFPDAVVVALSTFDLSAIPDPQGKQVVFACRSGKRSVSASLAAQAAGLPYDKHLDGGILGWKAAGLPTRSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STA2</accession>
    <name>Q3STA2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1227</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314518.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04489">
        <property type="protein sequence ID" value="ABA04489"/>
        <property type="gene ID" value="Nwi_1227"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1227"/>
    <dbReference type="eggNOG" id="ENOG50334RR">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_163848_0_0_5"/>
    <dbReference type="OMA" id="GIDQCLA"/>
    <dbReference type="OrthoDB" id="2048609at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229181" evidence="1">
        <location>
            <begin position="27"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="106" mass="11384" checksum="747491DC107F97EA" modified="2005-10-11" version="1">MHIFPAVRVFPGILAGTALFAAAAWADSRVFIVANQADGYGIDQCLAQGESCGAHAAHSYCRSRDFAQAVAYKRVRPDEMTDSIPGSISSCRHSGCNEYVAITCQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STB2</accession>
    <name>Q3STB2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">TRAP C4-dicarboxylate transport system permease DctM subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_1217</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314508.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1217"/>
    <dbReference type="EnsemblBacteria" id="ABA04479">
        <property type="protein sequence ID" value="ABA04479"/>
        <property type="gene ID" value="Nwi_1217"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1217"/>
    <dbReference type="eggNOG" id="COG4664">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019824_3_0_5"/>
    <dbReference type="OMA" id="LDPVWLG"/>
    <dbReference type="OrthoDB" id="941328at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010656">
        <property type="entry name" value="DctM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004681">
        <property type="entry name" value="TRAP_DctM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33362">
        <property type="entry name" value="PTHR33362"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06808">
        <property type="entry name" value="DctM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="15"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="58"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="105"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="145"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="184"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="253"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="285"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="349"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="388"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="434"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="474"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="DctM" evidence="4">
        <location>
            <begin position="12"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="536" mass="57645" checksum="D8204C7E7957B856" modified="2005-10-11" version="1">MITLEMMPPMMFGGLVLAMLIGFPVAFTLAAVGLSFGFLAIHLGFFDLNFLQAIPGRVFGGVLSNELLLAIPFFTFMGSVLERCGLAEDMLDSMGQLFGPIRGGLGYSVILVGFILGAITGTVAAQVIAMALISMPVMMRYGYNIRYVTGVLAASGTITQLVPPSLVLIVLADQLGKSVGDMYLGAWGPSLFQIMLFAGYTFLLGIFKPDHVPPVPKEFRTLTGWPLWRKCLMGIIPSAVLIFVVLGTMMLGLATPTEAGAMGAVGAIVLATIHHKDFSSTGRKLLIIGAITGGIGTLVGIFVAEGLVFKIAFAVTYLAVVWICLEAVRIPGLRDLIRQGYQSTMRLTTMVTFILIGSTCFSVVFLGVSGGEWLEHLLTSLPGGVWGFLIFINAFIFFLAFFLDFFEIAFIILPMLAPVAQKVLAPVVGADAALIWFGVMICVNMQTSFLHPPFGFALFYLRGVAPREVKSSDIYWGAVPWIGLQIIMVILVIAFPATVTGLLGKSTTVDLDNIKIEVPMIDVAPLDFGPVPTNRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STF6</accession>
    <name>Q3STF6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Gene transfer agent (GTA) like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1173</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314464.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1173"/>
    <dbReference type="EnsemblBacteria" id="ABA04435">
        <property type="protein sequence ID" value="ABA04435"/>
        <property type="gene ID" value="Nwi_1173"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1173"/>
    <dbReference type="eggNOG" id="ENOG5032YKK">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126531_0_0_5"/>
    <dbReference type="OMA" id="GGHKEAH"/>
    <dbReference type="OrthoDB" id="1910502at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021508">
        <property type="entry name" value="Gp17-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11367">
        <property type="entry name" value="DUF3168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="136" mass="14527" checksum="21AE88223AEA2DE6" modified="2005-10-11" version="1">MTSANAALRAAVHDALAADSGLHALLGGKRVYDEPPRGALLPYVAIGEARVTDVSVDEGPTQEHQITLHARSRRGGQSEAHAIAGAVLQALDDASLTLAAHRLVNFRFSVADIRRESDGRTYHAQVRFHAVTEPTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STH6</accession>
    <name>Q3STH6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flp/Fap pilin component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1153</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314445.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04415">
        <property type="protein sequence ID" value="ABA04415"/>
        <property type="gene ID" value="Nwi_1153"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1153"/>
    <dbReference type="eggNOG" id="COG3847">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_171854_3_3_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007047">
        <property type="entry name" value="Flp_Fap"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04964">
        <property type="entry name" value="Flp_Fap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="57" mass="5980" checksum="559BD780E6B9F25A" modified="2005-10-11" version="1">MRRLAKFASELLWDTSGATAIEYALIASGISIVIVAAVSGIGGSLRDRFDALNGLLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STR7</accession>
    <name>DNLJ_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA ligase</fullName>
            <ecNumber evidence="1">6.5.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Polydeoxyribonucleotide synthase [NAD(+)]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ligA</name>
        <name type="ordered locus">Nwi_1062</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NAD(+) + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide.</text>
            <dbReference type="EC" id="6.5.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD-dependent DNA ligase family. LigA subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314358.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3STR7"/>
    <dbReference type="SMR" id="Q3STR7"/>
    <dbReference type="STRING" id="323098.Nwi_1062"/>
    <dbReference type="PRIDE" id="Q3STR7"/>
    <dbReference type="EnsemblBacteria" id="ABA04324">
        <property type="protein sequence ID" value="ABA04324"/>
        <property type="gene ID" value="Nwi_1062"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1062"/>
    <dbReference type="eggNOG" id="COG0272">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007764_2_1_5"/>
    <dbReference type="OMA" id="HDVEHEI"/>
    <dbReference type="OrthoDB" id="241401at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003911">
        <property type="term" value="F:DNA ligase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00114">
        <property type="entry name" value="LIGANc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01588">
        <property type="entry name" value="DNA_ligase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041663">
        <property type="entry name" value="DisA/LigA_HHH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018239">
        <property type="entry name" value="DNA_ligase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033136">
        <property type="entry name" value="DNA_ligase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001679">
        <property type="entry name" value="DNAligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013839">
        <property type="entry name" value="DNAligase_adenylation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013840">
        <property type="entry name" value="DNAligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004150">
        <property type="entry name" value="NAD_DNA_ligase_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004149">
        <property type="entry name" value="Znf_DNAligase_C4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01653">
        <property type="entry name" value="DNA_ligase_aden"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03120">
        <property type="entry name" value="DNA_ligase_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03119">
        <property type="entry name" value="DNA_ligase_ZBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12826">
        <property type="entry name" value="HHH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001604">
        <property type="entry name" value="LigA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00532">
        <property type="entry name" value="LIGANc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="SSF52113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00575">
        <property type="entry name" value="dnlj"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01055">
        <property type="entry name" value="DNA_LIGASE_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01056">
        <property type="entry name" value="DNA_LIGASE_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000313337" description="DNA ligase">
        <location>
            <begin position="1"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="1">
        <location>
            <begin position="638"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="active site" description="N6-AMP-lysine intermediate" evidence="1">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="47"/>
            <end position="51"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="96"/>
            <end position="97"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="435"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="438"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="453"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="459"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01588"/>
        </source>
    </evidence>
    <sequence length="716" mass="78589" checksum="0AFC83DB828C9665" modified="2005-10-11" version="1">MAAKSKTPPPVDSLTRSQARVEHKRLALEIETHNERYYQQDAPTVSDATYDALQRRLEAIEARFPDLVTASSPTQTVGAAPARGFAKVQHAVPMLSLGNAFSDAEVAEFVERIRRFLKLGADDMPAIVAEPKIDGLSLSLRYENGDLVRAATRGDGFTGEDVTANVRTIADVPHKLNGHNIPTACELRGEVYMLKEDFLALNKKQQEAGDTVFANPRNSAAGSLRQKDVSITASRPLKFFAYAWGEMTDRPAETQNDMLEWLRDVGFIVNPLIQLCHSVDEVLAFYRRIGEQRATLGYDIDGVVYKVDRLDWQERLGFVSRSPRWAIAHKFAAEQAMTILRGIDIQVGRTGAMTPVARLEPVTVGGVVVQNATLHNEDYIRGLGNDGQPIRDGIDLRIGDTVVVQRAGDVIPQVVSVIIEKRPANAKRYEFPDRCPICGSHAVREEGEAVRRCTGALICPAQAVERLKHFVSRLAFDIDGLGEKQIQEFYDDGLVMHPVDIFTLQERDARAENKLRDRDGYGDVSVRNLFAAIDARRRIELNRLIFALGIRHVGEGNAKLLARHYGSIESFRSAMTEAAAAQTEAGNDSEAYADLTNIGGIGDIVADAVVEFFAEPRNVKALNDLLEEIEVLPVAQARSDSAVAGKTVVFTGSLEKFTRDEAKASAERVGAKTSSSVSKKTDLVVAGPGAGSKLKDAEKFGVKVISEDEWLKLIEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STT8</accession>
    <name>Q3STT8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1041</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04303">
        <property type="protein sequence ID" value="ABA04303"/>
        <property type="gene ID" value="Nwi_1041"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1041"/>
    <dbReference type="eggNOG" id="COG0741">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1188938_0_0_5"/>
    <dbReference type="OMA" id="PEDAWMQ"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="192" mass="22311" checksum="D110D73A9538C3FB" modified="2005-10-11" version="1">MRVDLALLGRIDAARQADERLDRFSVWSRWYDCQVEELRGRWMELFDFYKITARAPEDAWMQLATSLARDFVPGLLVENVFPTQDLLVEQARLFTLITEVSQAVLARRGEEESRDVSERMVLDQDFFNGESWDSAMFGKKPDSIDTFTARFSREKERFARLLIEQYWSKAAVMSFFADSGLEVPEFVAGLPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STU9</accession>
    <name>Q3STU9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1030"/>
    <dbReference type="EnsemblBacteria" id="ABA04292">
        <property type="protein sequence ID" value="ABA04292"/>
        <property type="gene ID" value="Nwi_1030"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1030"/>
    <dbReference type="eggNOG" id="COG2187">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1883575_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="135" mass="15331" checksum="9CD5E0D789089AC1" modified="2005-10-11" version="1">MRAMVLPVPHGLLWEKRRWRKEPPCFNPDLIPNMIDPTAQDAATQRDVLAFLATLTTYGGADDVRRIGTHAAVVFLAGDRVLKVKRAIRFPFLEYSTLEKRKVACEEENRTDHPALANAPRDRRQATAELRPRSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="130" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STV0</accession>
    <name>Q3STV0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_1029</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314325.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1029"/>
    <dbReference type="EnsemblBacteria" id="ABA04291">
        <property type="protein sequence ID" value="ABA04291"/>
        <property type="gene ID" value="Nwi_1029"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1029"/>
    <dbReference type="eggNOG" id="COG4191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_39_5"/>
    <dbReference type="OMA" id="NGIIMRI"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="5">
        <location>
            <begin position="14"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="5">
        <location>
            <begin position="141"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="6">
        <location>
            <begin position="209"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="288"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="511" mass="56894" checksum="A2BE550F1B29ED08" modified="2005-10-11" version="1">MTSDDLSSLNNLFRDEDLQRGIDKSGVGIWDLDLSTRKLFWSNPARRLFGVPENIPVSYDLFLSLLRRDDRARTKQAIQRSITTGCRFDLQYHIHGTLEGSRWIRARGGLVFNEDGTPQRLSGVILDIDHQKSIEEALIAREDHLRSILDTIPDAMIVIDDRGIMQHFSTAAERLFGYSEHEAIGQNVRILMPEPDSSRHDGYLARHQSTGERRIIGIGRVVTGKRRDGSTFPLHLSVGEMHSGGKRHFTGFVRDLTEHQQTQVKLQELQSELFHVSRLSAMGEMASALAHELNQPLTAISNYIKGSRRLLAASTDPDIAKIEMALDRAADQAIRAGQIIRRLRDFVSRRESEKRVESLSKLMEEASVLGLAGAREQNIILLTDLNADCDRVLVDRVQVQQVLVNLFRNALEAMAKSPKRELTASSTKAPDNMVELSISDTGHGFSEEAAANLFDTFFTTKESGMGVGLSISRSIVEAHGGRMWAETNDSGGATMRLTLPIASIGDFPDVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="133" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STX3</accession>
    <name>Q3STX3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Spermidine/putrescine import ATP-binding protein PotA</fullName>
            <ecNumber evidence="10">7.6.2.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">potA</name>
        <name evidence="12" type="ordered locus">Nwi_1006</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in beta-(1--&gt;2)glucan export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Part of the ABC transporter complex PotABCD involved in spermidine/putrescine import. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">The complex is composed of two ATP-binding proteins (PotA), two transmembrane proteins (PotB and PotC) and a solute-binding protein (PotD).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ABC transporter superfamily. Spermidine/putrescine importer (TC 3.A.1.11.1) family.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.11" evidence="10"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314306.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1006"/>
    <dbReference type="EnsemblBacteria" id="ABA04268">
        <property type="protein sequence ID" value="ABA04268"/>
        <property type="gene ID" value="Nwi_1006"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1006"/>
    <dbReference type="eggNOG" id="COG3842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_5"/>
    <dbReference type="OMA" id="EITVFWQ"/>
    <dbReference type="OrthoDB" id="1200451at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015417">
        <property type="term" value="F:ABC-type polyamine transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005893">
        <property type="entry name" value="Sp_pt_ABC_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013611">
        <property type="entry name" value="Transp-assoc_OB_typ2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08402">
        <property type="entry name" value="TOBE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="SSF50331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01187">
        <property type="entry name" value="potA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="20"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="52"/>
            <end position="59"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA04268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="382" mass="41315" checksum="42FEDEFCA3E7AD8E" modified="2005-10-11" version="1">MTDEARLGSEATAQADKPLLQVDAAVKRFGGFTAVDHLSLDIRTGEFFALLGPSGCGKTTLLRMLGGFETPDGGRILLDGQDISGVPPHRRPVNMMFQNYALFPHLSVRDNIAFGLRRAGMPRADIEARVAEMVALVSLEGMEKRKPEQLSGGQRQRVALARALARRPRLLLLDEPMAALDKKLRENTQAELMRLQRQLGMTFIIVTHDQEEAMMMADRIGVMNAGRLQQVATPRELYEVPNSRWIAEFVGGINIIDGAVESSGGGRITVATQAAGSVTVATPLQALTTDQVCVAIRPEKIRLSSGEAAPDAGDAAGTNRVEGVIAGVSYLGGQTRYQVRLDRGALLHASVANTVRREAEAFDTAQRVVACFAPDDCMVLDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="141" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STY7</accession>
    <name>Q3STY7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Two component, sigma54 specific, transcriptional regulator, Fis family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_0992</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314293.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0992"/>
    <dbReference type="EnsemblBacteria" id="ABA04254">
        <property type="protein sequence ID" value="ABA04254"/>
        <property type="gene ID" value="Nwi_0992"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0992"/>
    <dbReference type="eggNOG" id="COG2204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_0_6_5"/>
    <dbReference type="OMA" id="YRAVVMS"/>
    <dbReference type="OrthoDB" id="123059at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002197">
        <property type="entry name" value="HTH_Fis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002078">
        <property type="entry name" value="Sigma_54_int"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025943">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025944">
        <property type="entry name" value="Sigma_54_int_dom_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02954">
        <property type="entry name" value="HTH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00158">
        <property type="entry name" value="Sigma54_activat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00676">
        <property type="entry name" value="SIGMA54_INTERACT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00688">
        <property type="entry name" value="SIGMA54_INTERACT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50045">
        <property type="entry name" value="SIGMA54_INTERACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="10">
        <location>
            <begin position="4"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma-54 factor interaction" evidence="9">
        <location>
            <begin position="146"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="8">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA04254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="498" mass="53727" checksum="54FD6917697D2B37" modified="2005-10-11" version="1">MTATILIADDDAVQRRLVENMVRRCGYETIVVESGDAAVAAMTAGDGPVIDAVVLDLVMPGLDGMGVLAEIREAGLSIPVIVQTAHGGIDNVVSAMRAGAHDFVVKPVGIERLQVSLRNALNASALKNELQRVRHSREGRLTFGDIITRSEAMADVMKAAKKAAGSSIPVLIEGESGVGKELFARAIHGTSDRSAKPFVTVNCGAIPDNLVESILFGHEKGSFTGATDRHAGKFVDASGGTLFLDEVSELPLATQVKLLRALQEGAVEAVGGHKPVKVDVRIISATNRRLLDRVKAGLFREDLFYRLHVLPLTIPPLRKRREDIPNLLRHFLARFCAEENRLIKGISGEAMTALSQLDWPGNIRQLENAVYRAVVMSEDPQLGLPDFPSIAQQTAATTHAETHAPASEPLIMESIAQQTASQAIPGNEIPLAPAPDAGSLPLFAQDGEVRPLDELEADIIRFAVSHYRGQMSEVARRLKIGRSTLYRKLDDIAAKEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STZ9</accession>
    <name>Q3STZ9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_0980</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NodD regulates the expression of the nodABCFE genes which encode other nodulation proteins. NodD is also a negative regulator of its own expression. Binds flavonoids as inducers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314281.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0980"/>
    <dbReference type="EnsemblBacteria" id="ABA04242">
        <property type="protein sequence ID" value="ABA04242"/>
        <property type="gene ID" value="Nwi_0980"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0980"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_16_2_5"/>
    <dbReference type="OMA" id="HMTVSAI"/>
    <dbReference type="OrthoDB" id="1661500at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0536">Nodulation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="5">
        <location>
            <begin position="5"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA04242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="304" mass="32514" checksum="3323B4BD579C2111" modified="2005-10-11" version="1">MAKLPDFEALAIFAKVVELRSFAGAAADLGLSKATVSKAVSRLEDRLGARLFNRTSRRLALTDAGKNLSERAARLLADGEAAENEALARSTVPRGLVRLAVPMTFGVNVIAPLLPGFLAMYPEVSVDLHFSDAMVDLIGEGFDVGVRIASLPDSSLLARRLCPMPRHTVASPAYLKAHGRPTHPMHLAQHSCLGYAYLSTPGVWRYVNARGEQAAVRPSGRLRVNNGEALLPAIIAGLGIADLPEFIIGDAIAAKQVEVLLKGWKQVEGAVHLVMPPGGPRPARIEVLIDYLAHQLAHQGRRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU64</accession>
    <name>Q3SU64_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ABC-type Mn2+/Zn2+ transport systems permease components</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_0915</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ABC-3 integral membrane protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314218.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0915"/>
    <dbReference type="EnsemblBacteria" id="ABA04177">
        <property type="protein sequence ID" value="ABA04177"/>
        <property type="gene ID" value="Nwi_0915"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0915"/>
    <dbReference type="eggNOG" id="COG1108">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028808_4_2_5"/>
    <dbReference type="OMA" id="ALMGDGI"/>
    <dbReference type="OrthoDB" id="1421296at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001626">
        <property type="entry name" value="ABC_TroCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30477">
        <property type="entry name" value="PTHR30477"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00950">
        <property type="entry name" value="ABC-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA04177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="289" mass="30112" checksum="8703CFB27E580BCE" modified="2005-10-11" version="1">MIYDALVAPFADFEFMRRALAAAAALALGAAPIGVFLMLRGMSLVGDAMAHAILPGAAVGFLLSGLNLFAMTVGGLIAGFAVAILAGVVSRVTELKEDSSLATFYLASLALGVTIVSVKGTNIDLLHVLFGNILAMDDQTLMVIAINATVTLVVLAVIYRPLVFECVDPLFLRTVSRAGAPAHLAFLGLVVVNLVNGFHAMGTLLAVGLMILPAGIARFWSRDITGMMMIAVVSALLSGYAGLVLSFHSKVPSGPAIILVASVFYVLSVLFGRAGGIVRRFFPAPHLEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU76</accession>
    <name>Q3SU76_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0903</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314206.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0903"/>
    <dbReference type="EnsemblBacteria" id="ABA04165">
        <property type="protein sequence ID" value="ABA04165"/>
        <property type="gene ID" value="Nwi_0903"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0903"/>
    <dbReference type="eggNOG" id="COG4430">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170393_0_0_5"/>
    <dbReference type="OMA" id="CKHRERN"/>
    <dbReference type="OrthoDB" id="1891100at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="104" mass="12114" checksum="2ABDB5E7737ECBED" modified="2005-10-11" version="1">MANTFPHGTVHKAGDDLQAALRSDPKVFALWESLTPLGRNEFICWVEDAKQSRTRQRRIERTRDELLDGKKRPCCWTGCIHRADKAPSRWQQAVLIDQKRKKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUK6</accession>
    <name>Q3SUK6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cytochrome bo3 quinol oxidase subunit 1 apoprotein</fullName>
            <ecNumber evidence="9">1.10.3.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_0770</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the heme-copper respiratory oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.10.3.-" evidence="9"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314084.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0770"/>
    <dbReference type="EnsemblBacteria" id="ABA04035">
        <property type="protein sequence ID" value="ABA04035"/>
        <property type="gene ID" value="Nwi_0770"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0770"/>
    <dbReference type="eggNOG" id="COG0843">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011899_7_1_5"/>
    <dbReference type="OMA" id="FWGKMSF"/>
    <dbReference type="OrthoDB" id="316745at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070469">
        <property type="term" value="C:respirasome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.210.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023616">
        <property type="entry name" value="Cyt_c_oxase-like_su1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036927">
        <property type="entry name" value="Cyt_c_oxase-like_su1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000883">
        <property type="entry name" value="Cyt_C_Oxase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023615">
        <property type="entry name" value="Cyt_c_Oxase_su1_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422">
        <property type="entry name" value="PTHR10422"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00115">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01165">
        <property type="entry name" value="CYCOXIDASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81442">
        <property type="entry name" value="SSF81442"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50855">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00077">
        <property type="entry name" value="COX1_CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="101"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="188"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="224"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="272"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="307"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="348"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="380"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="413"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="452"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="486"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="586"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="COX1" evidence="8">
        <location>
            <begin position="39"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA04035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="657" mass="72016" checksum="4A323E247811E4B5" modified="2005-10-11" version="1">MLGKLTIDALPFYSGIAMAGALTVVCGGLGVAVLITWLGRWRYLWSEWLLSLDHKKIGIMYVILALIMLVRGVVDALMIRTQQAFAFEPHGYLPPDHFDQIFTSHATIMMFFVAMPFVLGLFSIIIPQQIGARQVAFPFMNAAGLWLTVSGAALVMISLVVGRFSAAYWTAYPPQSGIQFSPDVGVDYWIWAVLLSSIGAILAGISFLVTIVKRRAPGMKLMAMPLFTWTALCTSILIITAYPALIVAATLLGLDRHLGMQFFAGSNGVANYVSLFWIWGHPHVYILILPACGIYSEVVATFSGRRLFGYAALVYSMIAITVLSLVVWLHHFLTIGSAADVSAFFGRAAPLIAVPTIVTIVCWSLTIYRGEVRFSVPMLFTLGYIVTFLIGGITGFLFAVVPAGIHDTTFRVAHFHNLLIPGALFGYFAGYQYWFPKAFGFRLEEKWGRRAFWCWLVGFGLAFMPLYILGLMGMPQRLNRSDVAEWQPYLIAAQAGVLLIVCGVAFLAIQIAVSIRDRKALAASSNDPWNGRTLEWATASPPAAYNFAAVPPVQGIDPWWTMKQAGTADQGPARFHDIIMPGKSSAGVMLGAMSFVFAFAMIWHIWWLAAVGGLGMFAAVLSQTFHDHAGQRLPASDVEAIESRRLISPALGREAPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUN1</accession>
    <name>Q3SUN1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Trypsin-like serine protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0744</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314064.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0744"/>
    <dbReference type="EnsemblBacteria" id="ABA04010">
        <property type="protein sequence ID" value="ABA04010"/>
        <property type="gene ID" value="Nwi_0744"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0744"/>
    <dbReference type="eggNOG" id="COG0265">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060720_0_0_5"/>
    <dbReference type="OMA" id="NMVVPID"/>
    <dbReference type="OrthoDB" id="741829at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001940">
        <property type="entry name" value="Peptidase_S1C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00834">
        <property type="entry name" value="PROTEASES2C"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="SSF50494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="1">
        <location>
            <begin position="218"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="323" mass="34716" checksum="EF57012829DDD9F7" modified="2005-10-11" version="1">MASLTQWKVPSSAQPRPEDYRFDLDRTLSAVVGLHSFVPPDALTADTLGTERAGNGVVIDDGLVLTIGYVITEAETVWLHLADGRVVQADTVGVDRESGFGLVQALGDLDLEPLPLGSSAAASVNDPVVVAGVGGRSRSLAARIISREEFAGYWEYLIDQAIFTRPAHPNWGGTGLISASGDLIGIGSLQLEREEGGRSEHLNMNVPIDLLKPVLDDLRKFGKVNRPARPWLGIYVAEVENRVTAVGIVPKGPADRAELRAGDAILALKGEKVTDAAQLYRKLWALGPAGVDVPLTLHREGDTFDVVVTSTDRARMLKKPRLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUV3</accession>
    <name>Q3SUV3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0672</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0672"/>
    <dbReference type="EnsemblBacteria" id="ABA03938">
        <property type="protein sequence ID" value="ABA03938"/>
        <property type="gene ID" value="Nwi_0672"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0672"/>
    <dbReference type="eggNOG" id="ENOG502ZGWW">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1223692_0_0_5"/>
    <dbReference type="OrthoDB" id="1510796at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="226" mass="24665" checksum="D1A26C21824CC513" modified="2005-10-11" version="1">MTGFKVIAQTVRAHWSLLVLTSAGLVVVYYGAQIAVLYFRLGHLPNYITAYDYPANVARIIRSTPAVSDMIPIILNEWIFEIGYMDYDYGHGIAEWTMGIPPSKLLMIAIASVLISVNALLWRTARHSCFHLERRSLVGMTGLGAVTVGLVSISLSWVVCCGTPTWIVGLALLGLDVGLAFALEPFGVWIVSVGFIILAMSTLRFASHNLTVQSAVRRTGLLPEIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUX8</accession>
    <name>Q3SUX8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-acetylneuraminate synthase</fullName>
            <ecNumber evidence="2">2.5.1.56</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0647</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.5.1.56" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313973.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0647"/>
    <dbReference type="EnsemblBacteria" id="ABA03913">
        <property type="protein sequence ID" value="ABA03913"/>
        <property type="gene ID" value="Nwi_0647"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0647"/>
    <dbReference type="eggNOG" id="COG2089">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040465_0_1_5"/>
    <dbReference type="OMA" id="YRHRVEF"/>
    <dbReference type="OrthoDB" id="674753at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050462">
        <property type="term" value="F:N-acetylneuraminate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016051">
        <property type="term" value="P:carbohydrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006190">
        <property type="entry name" value="AFP_Neu5c_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036732">
        <property type="entry name" value="AFP_Neu5c_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013132">
        <property type="entry name" value="Neu5Ac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020030">
        <property type="entry name" value="Pseudaminic_synth_PseI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013974">
        <property type="entry name" value="SAF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03102">
        <property type="entry name" value="NeuB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08666">
        <property type="entry name" value="SAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00858">
        <property type="entry name" value="SAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51269">
        <property type="entry name" value="SSF51269"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03586">
        <property type="entry name" value="PseI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50844">
        <property type="entry name" value="AFP_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AFP-like" evidence="1">
        <location>
            <begin position="294"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="350" mass="37645" checksum="588A87DEC7B3D240" modified="2005-10-11" version="1">MQSPDLIIEGRKIGADHPPYIIAELSANHNGKLSKALEIIEAAAGAGADAVKIQTYTADTMTIPHDGEEFLIKGGLWDGYRLHQLYQQAHTPYEWHGQMFAKARELGITIFSSPFDETAVDLLAALDAPAYKIASFEVIDLPLIKYVAKQGKPMIISTGMANLGEIQEAAETARDNGAGGIALLHCTSAYPAPIEEANVRTVAHLGQAFNVVSGLSDHAPGSAACVASIALGGSIIEKHFTISRTDGGPDAAFSLEPHEFRRLVDDCKSAWSALGYVDYGLAQSEKANRQFRRSIYAVKPIKAGELLTSENIRVIRPGLGLPPKYLPRVIGRTAARDIAYGEAIKWSVIA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-09-11" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUZ0</accession>
    <name>EX7S_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Exodeoxyribonuclease 7 small subunit</fullName>
            <ecNumber evidence="1">3.1.11.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Exodeoxyribonuclease VII small subunit</fullName>
            <shortName evidence="1">Exonuclease VII small subunit</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">xseB</name>
        <name type="ordered locus">Nwi_0634</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterooligomer composed of large and small subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the XseB family.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.6" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313961.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SUZ0"/>
    <dbReference type="SMR" id="Q3SUZ0"/>
    <dbReference type="STRING" id="323098.Nwi_0634"/>
    <dbReference type="EnsemblBacteria" id="ABA03901">
        <property type="protein sequence ID" value="ABA03901"/>
        <property type="gene ID" value="Nwi_0634"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0634"/>
    <dbReference type="eggNOG" id="COG1722">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145918_0_3_5"/>
    <dbReference type="OMA" id="PLNDYKG"/>
    <dbReference type="OrthoDB" id="2057189at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009318">
        <property type="term" value="C:exodeoxyribonuclease VII complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008855">
        <property type="term" value="F:exodeoxyribonuclease VII activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00337">
        <property type="entry name" value="Exonuc_7_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003761">
        <property type="entry name" value="Exonuc_VII_S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037004">
        <property type="entry name" value="Exonuc_VII_ssu_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34137">
        <property type="entry name" value="PTHR34137"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02609">
        <property type="entry name" value="Exonuc_VII_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006488">
        <property type="entry name" value="Exonuc_VII_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116842">
        <property type="entry name" value="SSF116842"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01280">
        <property type="entry name" value="xseB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0269">Exonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000303729" description="Exodeoxyribonuclease 7 small subunit">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00337"/>
        </source>
    </evidence>
    <sequence length="83" mass="9281" checksum="7B8C4AF41D7BED8C" modified="2005-10-11" version="1">MAENVPADVNTLSFELAIEELETIVKRLEDGKVALEESVAIYERGEALKRRCEELLRRAEARVEKITTDASGHVTGTEPLDVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV18</accession>
    <name>Q3SV18_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transporter, CPA2 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0606</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313933.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0606"/>
    <dbReference type="EnsemblBacteria" id="ABA03873">
        <property type="protein sequence ID" value="ABA03873"/>
        <property type="gene ID" value="Nwi_0606"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0606"/>
    <dbReference type="eggNOG" id="COG0475">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005126_9_0_5"/>
    <dbReference type="OMA" id="KWSAKER"/>
    <dbReference type="OrthoDB" id="941006at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015299">
        <property type="term" value="F:solute:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="85"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="266"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="289"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="323"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="352"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="6">
        <location>
            <begin position="411"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="574" mass="61913" checksum="7326F7E02D1582D1" modified="2005-10-11" version="1">MHQLIGDITLCILFAWGLGLAAHFSRQPLILAYLLAGFFIGPFGMGLVKSQESIRTISELGLIFMLFMIGLEIDLKKIMRAGPVILIAGGGQLLGGCILGALFFVMIGLPSGGFDALYLCVACALSSTVIIVKVLYEKHELDTLPGRVTLGVLVLQDVFAILFLAVQPSLDDLHMSIVLLSIARVATLVATALIVSRYVLPFLFHQIARRPELVLLGALAWCFLIGEIAERLHLSREMGSLVAGVSLSTFPYALDVTAKVTTLRDFFITLFFVALGMTIPIPNGSVIGLALMIAAFTVASRLATTFLPLYLMRQGLRASLLPAINLAQISEFSLVVIQTGLIAGHIQSETASAASFAFVVLAVLSTFAIGRSDQLSRQAIRFLKRLGFRDLDRLQAGEGGHGAGAHGEPRRIVILGFFRAASALLNEIERRDEAMLDQIGVVDFNPLVFKTLSARGLQVTYGDISNVDTLVHAGVGRAEIIILSVPDSLLKGANNEKLVRHVRALNPTAKIISRADRLSDVEDQYAAGADYVTLTRLTDAQELFAAIEAAENGLLADKRAETDALLEGRREVLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV30</accession>
    <name>Q3SV30_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Exodeoxyribonuclease V</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0594</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313922.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0594"/>
    <dbReference type="EnsemblBacteria" id="ABA03861">
        <property type="protein sequence ID" value="ABA03861"/>
        <property type="gene ID" value="Nwi_0594"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0594"/>
    <dbReference type="eggNOG" id="COG0507">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_643819_0_0_5"/>
    <dbReference type="OMA" id="TIHKKIY"/>
    <dbReference type="OrthoDB" id="961809at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027785">
        <property type="entry name" value="UvrD-like_helicase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13538">
        <property type="entry name" value="UvrD_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD_C_2" evidence="1">
        <location>
            <begin position="320"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="369" mass="41217" checksum="BF8742495C095B09" modified="2005-10-11" version="1">MTSFTPHQDTALKAVADWLKAKPGRNGTPAVFRLFGYAGTGKTTLARHIADGVDGEVKFAAFTGKAALVMRNKGCGGASTIHSLIYRTRESGEEQPSFELWDDAPASKARLIVIDECSMVDAELGRDLMSFDCPLLVLGDPAQLPPIQGAGFFTNASPDAMLTEVHRQAKDDPIVRMSMDVREGRELDIGRYGESEVVPRSELDPARVMDADQVLVGRNNTRRAYNMRMRQRQNIEDPLPVAGDKLVCLRNNRKKALFNGGLWRVKARAVSRSKIITMRLSPDEEFAHKVTKVSVRQDCFAGGIEDIPWEQRKPYDEFDYGYVLTVHKSQGSQWDDVVLFDESFAFQDSRARWLYTGITRAAKRLSVVV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-01-10" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV41</accession>
    <name>HIS8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Histidinol-phosphate aminotransferase</fullName>
            <ecNumber evidence="1">2.6.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Imidazole acetol-phosphate transaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisC</name>
        <name type="ordered locus">Nwi_0583</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-oxoglutarate + L-histidinol phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23744"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:57980"/>
            <dbReference type="EC" id="2.6.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 7/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. Histidinol-phosphate aminotransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.9" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313911.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SV41"/>
    <dbReference type="SMR" id="Q3SV41"/>
    <dbReference type="STRING" id="323098.Nwi_0583"/>
    <dbReference type="EnsemblBacteria" id="ABA03850">
        <property type="protein sequence ID" value="ABA03850"/>
        <property type="gene ID" value="Nwi_0583"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0583"/>
    <dbReference type="eggNOG" id="COG0079">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_3_3_5"/>
    <dbReference type="OMA" id="YPDMACT"/>
    <dbReference type="OrthoDB" id="1248286at2"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00012"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004400">
        <property type="term" value="F:histidinol-phosphate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01023">
        <property type="entry name" value="HisC_aminotrans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005861">
        <property type="entry name" value="HisP_aminotrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01141">
        <property type="entry name" value="hisC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0032">Aminotransferase</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000153401" description="Histidinol-phosphate aminotransferase">
        <location>
            <begin position="1"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="365" mass="39892" checksum="616F0AB70720450D" modified="2005-10-11" version="1">MSRPVPNPGILDIAPYTPGKSPAPEAGRKVFKLSANETPFGPSPKAMDAYRDAVAHLEDYPEGTSRVLRAAIGRAYGLDPDRIICGAGSDEILNLLAHTFLSHGDEAISTTHAFLVYPIATMANGATNIVAPETGYTADIDAILERVTPKTKMVWLANPNNPTGTYLPFDEIRRLRAGLPPHVLLVLDGAYSDYVSRNDYEFGIELVATTENTVLTHTFSKIHGLAALRIGWMFGPAHIVDAMNRIRGPFNVSTPAMLAAAAAIEDTAHVQMTRMHTEKWRSWLTDELTNLGLKVTPSVTNFILIHFPDARGRTASEADAFLTRRGLVLRALDNYCLPNALRMTIGTEEANRLVADGLRDFMART</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV46</accession>
    <name>Q3SV46_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF218 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0578</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0578"/>
    <dbReference type="EnsemblBacteria" id="ABA03845">
        <property type="protein sequence ID" value="ABA03845"/>
        <property type="gene ID" value="Nwi_0578"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0578"/>
    <dbReference type="eggNOG" id="COG1434">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080658_0_0_5"/>
    <dbReference type="OMA" id="CCVDLDY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06259">
        <property type="entry name" value="YdcF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003848">
        <property type="entry name" value="DUF218"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02698">
        <property type="entry name" value="DUF218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="DUF218" evidence="2">
        <location>
            <begin position="62"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="241" mass="25903" checksum="519A4CD41BC39AEA" modified="2005-10-11" version="1">MSKEARHDNVEAPRRAPGRRRGMLLATLVAAAGIAFVGAAVGFVGFLSQLRGVETKPAANADGIVVLTGGSSRISDAVDLLAGGYGKRLLISGVHPTNGVSDISRSLPDSQRLLTCCVDLDRSAVNTRSNASETRRWVDGQGFRSLIVVTSNYHMPRAIVELSHAMPDVTLIPFAVIGEKWREEPWWTSGATLRLLLSEYVKYLAAEARVRLAHLGLDLELPPENADQSTGAIQPRRPASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV48</accession>
    <name>Q3SV48_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Cell division ATP-binding protein FtsE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ftsE</name>
        <name evidence="10" type="ordered locus">Nwi_0576</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in beta-(1--&gt;2)glucan export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Part of the ABC transporter FtsEX involved in cellular division. Important for assembly or stability of the septal ring.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer. Forms a membrane-associated complex with FtsX.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell inner membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
            <orientation evidence="8">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313904.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0576"/>
    <dbReference type="EnsemblBacteria" id="ABA03843">
        <property type="protein sequence ID" value="ABA03843"/>
        <property type="gene ID" value="Nwi_0576"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0576"/>
    <dbReference type="eggNOG" id="COG2884">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_5"/>
    <dbReference type="OMA" id="MRDEIMD"/>
    <dbReference type="OrthoDB" id="1181903at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005286">
        <property type="entry name" value="Cell_div_FtsE_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02673">
        <property type="entry name" value="FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="2"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA03843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="219" mass="24638" checksum="6753A6E488005E51" modified="2005-10-11" version="1">MIRFENVGLRYGLGPEILRDISFHLPARSFQFLSGPSGAGKTSLLRLLFLSLRPTRGLVNLFGQDVSLQSKDAIADLRKRIGIVLQDFRLLDHMTTYENVALPFRVMGREESTYRREVIDLLKWVGLGERMDALPPVLSGGEKQRAAIARAVIARPQLLLADEPTGNVDPTLGQRLLRLFIELNKSGTAVIIATHDIALMDQYDARRLVLHQGRLHIYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV61</accession>
    <name>Q3SV61_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0563</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313891.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03830">
        <property type="protein sequence ID" value="ABA03830"/>
        <property type="gene ID" value="Nwi_0563"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0563"/>
    <dbReference type="eggNOG" id="ENOG5033B4H">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153166_0_0_5"/>
    <dbReference type="OMA" id="FCYRRSH"/>
    <dbReference type="OrthoDB" id="2068456at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="131" mass="14195" checksum="A5555E389E418E34" modified="2005-10-11" version="1">MSSSDAFENDICPIKDDLLGAMYRASEHGLPQLIASVAPDVRAMLALFCYRRSHLHALALAVASSCTERDLIVLGGRVGSTLFALSRNARQPHVRSPHGQRKPITLSTRPLSTYVPLDDLADDEEPALVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVA8</accession>
    <name>Q3SVA8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0516</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313844.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0516"/>
    <dbReference type="EnsemblBacteria" id="ABA03783">
        <property type="protein sequence ID" value="ABA03783"/>
        <property type="gene ID" value="Nwi_0516"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0516"/>
    <dbReference type="eggNOG" id="COG5400">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077638_1_0_5"/>
    <dbReference type="OMA" id="WGLPNGY"/>
    <dbReference type="OrthoDB" id="1645607at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008325">
        <property type="entry name" value="EipA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06577">
        <property type="entry name" value="EipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF033924">
        <property type="entry name" value="UCP033924"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229177" evidence="2">
        <location>
            <begin position="25"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="214" mass="22727" checksum="AB88CC0D062F6EF9" modified="2005-10-11" version="1">MTFISRLAAVALAAVMFAIVPASAQQSAPYQTPPAQPYQAQPYQAQPAHRNGSPGTYSSDELLKSGTRFFGNVSRGLASVIERAVSQWGLPNGYILGEEGSGAFVAGLRYGEGTLYTKNAGDLKVYWQGPSVGFDWGGDGARTMTLVYNLPATNAIYQRFVGIDGSAYIVGGFGMTALTANDIVLVPIRSGLGLRLGANVGYLKYTPQATWNPF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVD0</accession>
    <name>ISPG_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)</fullName>
            <ecNumber evidence="1">1.17.7.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ispG</name>
        <name type="ordered locus">Nwi_0494</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) + H2O + oxidized [flavodoxin] = 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + reduced [flavodoxin]</text>
            <dbReference type="Rhea" id="RHEA:43604"/>
            <dbReference type="Rhea" id="RHEA-COMP:10622"/>
            <dbReference type="Rhea" id="RHEA-COMP:10623"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:58483"/>
            <dbReference type="ChEBI" id="CHEBI:128753"/>
            <dbReference type="EC" id="1.17.7.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 5/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IspG family.</text>
    </comment>
    <dbReference type="EC" id="1.17.7.3" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313822.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVD0"/>
    <dbReference type="SMR" id="Q3SVD0"/>
    <dbReference type="STRING" id="323098.Nwi_0494"/>
    <dbReference type="EnsemblBacteria" id="ABA03761">
        <property type="protein sequence ID" value="ABA03761"/>
        <property type="gene ID" value="Nwi_0494"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0494"/>
    <dbReference type="eggNOG" id="COG0821">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042258_1_0_5"/>
    <dbReference type="OMA" id="PTCGRTQ"/>
    <dbReference type="OrthoDB" id="665306at2"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00096"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046429">
        <property type="term" value="F:4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.413.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00159">
        <property type="entry name" value="IspG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016425">
        <property type="entry name" value="IspG_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004588">
        <property type="entry name" value="IspG_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045854">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30454">
        <property type="entry name" value="PTHR30454"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04551">
        <property type="entry name" value="GcpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004640">
        <property type="entry name" value="IspG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56014">
        <property type="entry name" value="SSF56014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00612">
        <property type="entry name" value="ispG_gcpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000011490" description="4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)">
        <location>
            <begin position="1"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00159"/>
        </source>
    </evidence>
    <sequence length="430" mass="45755" checksum="5A9B8DFD2BD09D35" modified="2005-10-11" version="1">MNKLESPSEIDVAGPSPRHKTTQVMVGNVAVGGGAPIVVQSMTNTDTADIEGTIAQVAALSRAGSEMVRMTVDRDEAAAAVPHIRDGLRKRGITTPLIGDFHYIGHKLLADHPSCAEALDKYRINPGNVGFKDKRDKQFTDIVETAIKYGKAVRIGANWGSLDQELLTHLMEENANSAAPLDARAVTREAMVQSALLSAKRAEEIGLPKTRMVLSAKVSAVQDLIAVYQTLASRSDYAIHLGLTEAGMGSKGIVASSAALGILLQQGIGDTIRISLTPEPGGDRTLEVQVAQELLQTMGFRTFVPLVAACPGCGRTTSTTFQELARSIQDFIRDEMPNWKTQYPGVEQLNVAVMGCIVNGPGESKHADIGISLPGTGEAPAAPVFVDGKKFKTLRGPAIAQDFKALVIDYIEQRYGDAAKARAEAVSAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVG3</accession>
    <name>Q3SVG3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0461</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0461"/>
    <dbReference type="EnsemblBacteria" id="ABA03728">
        <property type="protein sequence ID" value="ABA03728"/>
        <property type="gene ID" value="Nwi_0461"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0461"/>
    <dbReference type="eggNOG" id="COG1236">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050517_1_0_5"/>
    <dbReference type="OMA" id="FYIDPWR"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026360">
        <property type="entry name" value="Xnuc_lig_assoc"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04122">
        <property type="entry name" value="Xnuc_lig_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="390" mass="41958" checksum="7815708DFCDD6D2B" modified="2005-10-11" version="1">MGDYFKVGDDDPSRVFRPGCAPFPPPDRSKARTILVAAYMSLMRPQDILMPDAAGLYCKPGGFHIDPVRPVDRAIVTHGHSDHARPGHGAVLATPETLDIMRLRYGDDFAGSAQAISYGQEIRLGDVTIRFHPAGHVLGSAQIAVTHGGIRIVISGDYKDVGDPTCTPLEIVPCDVFITEATFGLPVFRHGDAASETGKLLASVALFPERAHLVGAYSLGKAQRVIAMVRAAGYDAPIYLHGAMEKVTQYYRSRGIALGDLRPARGMKKADFAGTITLAPPSATADVWTRRFPDPVRAFASGWMRVRARARQRRVELPLVISDHADWNGLTRTIAATGAGEIWVTHGQEDALVHWCRAQGLAARPLALVGYGDEDGEQDAAAFSGGEDGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVK2</accession>
    <name>Q3SVK2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">2-oxoglutarate dehydrogenase E1 component</fullName>
            <ecNumber evidence="5">1.2.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Alpha-ketoglutarate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_0422</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">E1 component of the 2-oxoglutarate dehydrogenase (OGDH) complex which catalyzes the decarboxylation of 2-oxoglutarate, the first step in the conversion of 2-oxoglutarate to succinyl-CoA and CO(2).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer. Part of the 2-oxoglutarate dehydrogenase (OGDH) complex composed of E1 (2-oxoglutarate dehydrogenase), E2 (dihydrolipoamide succinyltransferase) and E3 (dihydrolipoamide dehydrogenase); the complex contains multiple copies of the three enzymatic components (E1, E2 and E3).</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the alpha-ketoglutarate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.2" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313753.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0422"/>
    <dbReference type="EnsemblBacteria" id="ABA03689">
        <property type="protein sequence ID" value="ABA03689"/>
        <property type="gene ID" value="Nwi_0422"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0422"/>
    <dbReference type="eggNOG" id="COG0567">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004709_1_0_5"/>
    <dbReference type="OMA" id="RDSYCRT"/>
    <dbReference type="OrthoDB" id="29166at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004591">
        <property type="term" value="F:oxoglutarate dehydrogenase (succinyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032106">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011603">
        <property type="entry name" value="2oxoglutarate_DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031717">
        <property type="entry name" value="KGD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042179">
        <property type="entry name" value="KGD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152">
        <property type="entry name" value="PTHR23152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16078">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16870">
        <property type="entry name" value="OxoGdeHyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000157">
        <property type="entry name" value="Oxoglu_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00239">
        <property type="entry name" value="2oxo_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Transket_pyr" evidence="11">
        <location>
            <begin position="632"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="52"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA03689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="985" mass="110867" checksum="2D721E03B02F2180" modified="2005-10-11" version="1">MSRQDANAAFALSSFLQGANAGYIDELYARYEKDPGSVDAAWQEFFKSLKDSPQDVEKNARGPSWERANWPQAPRDDLTSALDGNWARFEKTADVKAQETAKPKASVLAGGDVNQATRDSVRALMLIRAYRMRGHFHARLDPLGLEPARDREELDPRAYGFTEADMDRKIFLDHVLGLEYGSLREIVAICQRTYCQTLGVEFMHISDAAQKSWIQERIEGPDKEISFTPEGRRAILIKLIEAEGFEKFCDVKFTGTKRFGLDGGESLIPALEQIIKRGGNLGVKEIVIGMPHRGRLNVLTQVMGKPHRALFHEFKGGSVNPDSVEGSGDVKYHLGASSDREFDNNRIHLSLTANPSHLEIVDPVVLGKVRAKQDQHGDPPEQRNSVLPLLMHGDAAFAGQGVVAECFALSDLKGYRTGGSIHFIVNNQIGFTTYPRYSRSSPYPSDVAKMIDAPIFHVNGDDPEAVVFAAKVAVEFRQKFHKPVVIDMFCYRRHGHNEGDEPSFTNPLMYKKIAVHPSTLELYAKRLSAEGIITEGEIEKLKADWRARLDAELEAGAGYRPNKADWLDGKWAGLKLADQSEEPRRGVTGVSIETLKKIGRSITRVPDGFRVHRTVQRFLDNRARAIESGAGIDWATAEALAFCTLMLEGHNVRLSGQDSERGTFSQRHSVLFDQEDESRYTPFNHLDADHGCYEVINSLLSEEAVLGFEYGYSLAEPNTLTMWEAQFGDFANGAQVLFDQFISSGERKWLRMSGLVCMLPHGYEGQGPEHSSARLERFLQMCAEDNMQVVNITTPANYYHALRRQLKRQIRKPLIMMTPKSLLRHKRVVSRLDELGADTAFHRILYDDATMLPEQKIRLVDDDKIRRVVLCSGKVYYDLYEEREQRDINDVYLMRIEQLYPVPLKALVQVLGSFKNAEIVWCQEEPRNMGAWLFIEPYLEWVLNQIGAPGKRPRYVGRAAAAATATGLMSKHLAQLKAFLDEALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVR2</accession>
    <name>Q3SVR2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, AsnC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0362</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313694.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0362"/>
    <dbReference type="EnsemblBacteria" id="ABA03629">
        <property type="protein sequence ID" value="ABA03629"/>
        <property type="gene ID" value="Nwi_0362"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0362"/>
    <dbReference type="eggNOG" id="COG1522">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_0_2_5"/>
    <dbReference type="OMA" id="HVSGDYD"/>
    <dbReference type="OrthoDB" id="1844486at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019885">
        <property type="entry name" value="Tscrpt_reg_HTH_AsnC-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13404">
        <property type="entry name" value="HTH_AsnC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00519">
        <property type="entry name" value="HTH_ASNC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="15"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="169" mass="18588" checksum="7949E4095B654B80" modified="2005-10-11" version="1">MSDIAPQIIETGRRLDAIDRKILTVLQEDASLSVAEIGDRVGLSSTPCWKRIQRLEADGIILRRVALVDQNKIGLGISVFVSVESGDHSDVWLKKFADAISAMPEVMELYRMAGDVDYMLRVVVADMASYDLFYKKLISSVALKNVTSRFAMEKIKSVTALPVPALSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVS6</accession>
    <name>Q3SVS6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two component transcriptional regulator, winged helix family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0348</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313680.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0348"/>
    <dbReference type="EnsemblBacteria" id="ABA03615">
        <property type="protein sequence ID" value="ABA03615"/>
        <property type="gene ID" value="Nwi_0348"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0348"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_4_5"/>
    <dbReference type="OMA" id="WGGDTRV"/>
    <dbReference type="OrthoDB" id="817710at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="3"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="4">
        <location>
            <begin position="133"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="133"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="233" mass="26548" checksum="728251A109666FED" modified="2005-10-11" version="1">MPTIALVDDDRNILTSVSIALEAEGYRIMTYTDGASALDGFRTSQPDLAILDIKMPRMDGMETLRRLRQKSDLPVIFLTSKDEEIDELFGLKMGADDFIRKPFSQRLLVERVKAVLRRACPKDPAAAPKESDAKALDRGLLRMDPERHTCTWKNEPVTLTVTEFLILQALATRPGVVKSRNALMDAAYDDQVYVDDRTIDSHIKRLRKKFKVVDDDFEMIETLYGVGYRFKET</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVV5</accession>
    <name>Q3SVV5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Heme exporter protein D</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_0319</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Required for the export of heme to the periplasm for the biogenesis of c-type cytochromes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 11">Cell inner membrane</location>
            <topology evidence="3 11">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the CcmD/CycX/HelD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313651.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0319"/>
    <dbReference type="EnsemblBacteria" id="ABA03586">
        <property type="protein sequence ID" value="ABA03586"/>
        <property type="gene ID" value="Nwi_0319"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0319"/>
    <dbReference type="eggNOG" id="ENOG5033CGS">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_199071_0_0_5"/>
    <dbReference type="OMA" id="PHAAFIW"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015886">
        <property type="term" value="P:heme transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007078">
        <property type="entry name" value="Haem_export_protD_CcmD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04995">
        <property type="entry name" value="CcmD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03141">
        <property type="entry name" value="cytochro_ccmD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="7 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA03586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="59" mass="6382" checksum="DAA4C9333D4C2B4D" modified="2005-10-11" version="1">MALGPYASFIVTSYVSVAAVVVALIVWVIMDYRRQKARLRGLEAAGAVRRSGRSAAEVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW01</accession>
    <name>Q3SW01_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0273</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313606.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0273"/>
    <dbReference type="EnsemblBacteria" id="ABA03540">
        <property type="protein sequence ID" value="ABA03540"/>
        <property type="gene ID" value="Nwi_0273"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0273"/>
    <dbReference type="eggNOG" id="ENOG503444T">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2410271_0_0_5"/>
    <dbReference type="OrthoDB" id="2059875at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="92" mass="10601" checksum="640EDB8F33FB1A71" modified="2005-10-11" version="1">MKNGRGDFEPQGLTISYKELGTISIDELMHALIEDIHALKDLYNVQYVKGPRLRLFVTNEYGEVVRVRRPGGGSISYMDTHHYRPACRDYDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW20</accession>
    <name>Q3SW20_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Helix-turn-helix, Fis-type</fullName>
            <ecNumber evidence="4">2.7.7.49</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0254</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ParB family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.49" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0254"/>
    <dbReference type="EnsemblBacteria" id="ABA03521">
        <property type="protein sequence ID" value="ABA03521"/>
        <property type="gene ID" value="Nwi_0254"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0254"/>
    <dbReference type="eggNOG" id="COG1475">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2963">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_357085_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004437">
        <property type="entry name" value="ParB/RepB/Spo0J"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB/Sulfiredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010921">
        <property type="entry name" value="Trp_repressor/repl_initiator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02195">
        <property type="entry name" value="ParBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00470">
        <property type="entry name" value="ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="SSF110849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48295">
        <property type="entry name" value="SSF48295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00180">
        <property type="entry name" value="parB_part"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="3">
        <location>
            <begin position="591"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="343"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="404"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="437"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="785" mass="87925" checksum="8A1D376DFA9F2B8B" modified="2005-10-11" version="1">MSIQTIPLQSILPPQGNPRSAFDPATIEGLAASIRQDGLLQNLVVRPSRGRQFRIVSGERRYRALKLLQERGDIAEDFGVTVEIRSKLSKDDALRLATVENVQRENLPPLDEAAAFAALIRKGASLDDLAAKTGLSHTTIRRRLALNDLCREAKEALAAGTITLAQAEALTLGDADAQRSVLGDLESGYVPYSAADIRDHLLDERPSVAMAIFPVEQYTGTITTDLFADDEESYFDDADQFLELQRQAVEKLATEYAGKAAWVEVTEAHRIPAWQYEEAEDGEQGGVLINLAPSGRVEIREGLVSPACPPSGLLRQFRSFREGRISGSSQPIWSADETEIRTRQSAGRTGAEGHPTADAPALFGGGEDPYRAGRTARRGEHLRALPPRRHRRLDVLRLVQGVPGSRQAPVGGRHGTCRNLWRGERSSSGSLGVEGSRRRSHPGEPSAKKKHERGWGERGMRYPASEKAEIIALVEQSHLPAKRTLDKLGIPRATFYRWYDRYRAGGIEALADHRSRPDRVWNRIPDDVRGQIIDLALELPELSPRELAVRFTDERKYFVSEASVYRLLKAHDLITSPAYVVIKAANEFKDKTTAANQLWQTDFTYLKITGWGWYYLSTVLDDFSRYIVAWRLGPTMCASDVTATLDQALAASGLDHVSVRQRPRLLSDNGSSYVADDLATWLRAKDMQHVRGAPYHPQTQGKIERWHQTLKNRILLENYYLPDDLKRQVAAFVEHYNHDRYHESIGNVTPADVYFGRAETILAERHRIKRDTIANRRLQHQLQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW99</accession>
    <name>Q3SW99_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Lipopolysaccharide export system ATP-binding protein LptB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">Nwi_0174</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in beta-(1--&gt;2)glucan export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Part of the ABC transporter complex LptBFG involved in the translocation of lipopolysaccharide (LPS) from the inner membrane to the outer membrane. Probably responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the lipopolysaccharide transport and assembly complex. The LptBFG transporter is composed of two ATP-binding proteins (LptB) and two transmembrane proteins (LptF and LptG).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell inner membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ABC transporter superfamily. Outer membrane lipopolysaccharide export (TC 1.B.42) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313511.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0174"/>
    <dbReference type="EnsemblBacteria" id="ABA03442">
        <property type="protein sequence ID" value="ABA03442"/>
        <property type="gene ID" value="Nwi_0174"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0174"/>
    <dbReference type="eggNOG" id="COG1137">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_5"/>
    <dbReference type="OMA" id="LPMYQRA"/>
    <dbReference type="OrthoDB" id="1220708at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03218">
        <property type="entry name" value="ABC_YhbG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032823">
        <property type="entry name" value="BCA_ABC_TP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030921">
        <property type="entry name" value="LPS_export_LptB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12399">
        <property type="entry name" value="BCA_ABC_TP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04406">
        <property type="entry name" value="LPS_export_lptB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="96"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="38"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="128"/>
            <end position="135"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00026081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ABA03442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="332" mass="36407" checksum="A841D4BCABFA9AF4" modified="2005-10-11" version="1">MVDIFNVFRKRPQKRAARGIARTRTDITALGDAIGDMLTHPVRDAPPVARGSRRDAPPSRAPARPAPETSRPEAARLRAASSGTAAPRMATRPGYLAVHGVEKSFGSRQVVRGVSIYVRRGEAVGLLGPNGAGKTTVFYMITGLIPADRGAIELDGHDVTRLPMYQRARLGIGYLPQEASIFRGLSVEQNIRAVLEVVEPNKKKREAELDALLEEFNITRLRKSPSIALSGGERRRVEIARALATRPNYMLLDEPFAGIDPIAVGDIQDLVRHLTNRGIGVLITDHNVRETLGLTDRAYIVYAGEILTEGSPEAIVNDPDVRRLYLGEEFRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWA9</accession>
    <name>Q3SWA9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glutathione S-transferase</fullName>
            <ecNumber evidence="3">2.5.1.18</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0164</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.5.1.18" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313501.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0164"/>
    <dbReference type="EnsemblBacteria" id="ABA03432">
        <property type="protein sequence ID" value="ABA03432"/>
        <property type="gene ID" value="Nwi_0164"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0164"/>
    <dbReference type="eggNOG" id="COG0625">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011226_5_3_5"/>
    <dbReference type="OMA" id="ERVYKLQ"/>
    <dbReference type="OrthoDB" id="1819702at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="SSF47616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="2">
        <location>
            <begin position="88"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="230" mass="26274" checksum="C2DE40E873ED88CA" modified="2005-10-11" version="1">MFTLLHHPFCPQSRFIRLILGEHGLDVRPVEERVWERREAFLRLNPAGTTPVLIAEGIPAVPGAAIIADYLDETYGERVGERRLLPTSMPERIEVRRLMAWFNEKFFEEVSSPLVTERIYKRFMAEEIGGGPPETDVIRAARTNVRYHLAYIGWLARTRNYLAGDRLTYADLAAAAHLSAIDYLGDVPWAEDEAAKAWYARIKSRPSFRPLLSEWLAGVPASRTYVDLDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWD3</accession>
    <name>Q3SWD3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">dCTP deaminase</fullName>
            <ecNumber evidence="1">3.5.4.13</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0140</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.4.13" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313477.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0140"/>
    <dbReference type="EnsemblBacteria" id="ABA03408">
        <property type="protein sequence ID" value="ABA03408"/>
        <property type="gene ID" value="Nwi_0140"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0140"/>
    <dbReference type="eggNOG" id="COG0717">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043743_0_0_5"/>
    <dbReference type="OMA" id="ETNCVYI"/>
    <dbReference type="OrthoDB" id="696035at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008829">
        <property type="term" value="F:dCTP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009394">
        <property type="term" value="P:2'-deoxyribonucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010550">
        <property type="entry name" value="dCTP_deam_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06559">
        <property type="entry name" value="DCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="SSF51283"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="368" mass="40009" checksum="586F55D4A526912E" modified="2005-10-11" version="1">MSFTLPRDANGILPDRMIAAMADAGLILPEYPFVESQIQPASLDLRLGATAYRVRASFLPGPDATVAERIDELKLHEIDLSDGAVLETNCVYIVPLLESLALPPEIVAAANPKSSTGRLDVFTRVIADGTRSFDMIGAGYHGPLYAEISPKTFPVLLREGSRLSQVRFRSGDAVLDADELDALHASERLVDSDDTDFVNGVALSVDLSGAQAHGFVGYRAKRHTGVIDIDRRGGYAVGEFWEPIAARPDASLILDPGEFYILASKEAVQVPPDYAAEMVPFDPLVGEFRVHYAGFFDPGFGYAGAGGEGSRAVLEVRSREVPFILEHGQIVGRLVYEKMLARPDAMYGQRIGSNYQAQGLKLSKHFRL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWK5</accession>
    <name>RL35_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L35</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpmI</name>
        <name type="ordered locus">Nwi_0068</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL35 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009796600.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWK5"/>
    <dbReference type="SMR" id="Q3SWK5"/>
    <dbReference type="STRING" id="323098.Nwi_0068"/>
    <dbReference type="EnsemblBacteria" id="ABA03336">
        <property type="protein sequence ID" value="ABA03336"/>
        <property type="gene ID" value="Nwi_0068"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0068"/>
    <dbReference type="eggNOG" id="COG0291">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_169643_2_1_5"/>
    <dbReference type="OMA" id="KQWAPYG"/>
    <dbReference type="OrthoDB" id="2046660at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00514">
        <property type="entry name" value="Ribosomal_L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021137">
        <property type="entry name" value="Ribosomal_L35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018265">
        <property type="entry name" value="Ribosomal_L35_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001706">
        <property type="entry name" value="Ribosomal_L35_non-mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037229">
        <property type="entry name" value="Ribosomal_L35_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01632">
        <property type="entry name" value="Ribosomal_L35p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00064">
        <property type="entry name" value="RIBOSOMALL35"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143034">
        <property type="entry name" value="SSF143034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00001">
        <property type="entry name" value="rpmI_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00936">
        <property type="entry name" value="RIBOSOMAL_L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000258714" description="50S ribosomal protein L35">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="66" mass="7636" checksum="87E73C514C5073F1" modified="2005-10-11" version="1">MPKLKTKSGAKKRFKITATGKVKHAQRGKRHGMIKRTKKQIRQLRGTRVLFKTDGDNIKKYFLPNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWM6</accession>
    <name>Q3SWM6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PilZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0047</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313386.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0047"/>
    <dbReference type="EnsemblBacteria" id="ABA03315">
        <property type="protein sequence ID" value="ABA03315"/>
        <property type="gene ID" value="Nwi_0047"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0047"/>
    <dbReference type="eggNOG" id="ENOG50348C5">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_160508_0_0_5"/>
    <dbReference type="OMA" id="MMAIDGT"/>
    <dbReference type="OrthoDB" id="1800377at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035438">
        <property type="term" value="F:cyclic-di-GMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009875">
        <property type="entry name" value="PilZ_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07238">
        <property type="entry name" value="PilZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PilZ" evidence="1">
        <location>
            <begin position="4"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="106" mass="11946" checksum="3AABBDC51C4D472B" modified="2005-10-11" version="1">MGTERRKGERVTFERGYSAHMMGIDGTWRRNCTMEDVSEIGAKLTVEGSIEGLHLKEFFLLLSSTGLAYRRCELAWVNGDQIGVNFLKQTDKKKKTTRQNSATADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWN7</accession>
    <name>Q3SWN7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0036</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313375.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0036"/>
    <dbReference type="EnsemblBacteria" id="ABA03304">
        <property type="protein sequence ID" value="ABA03304"/>
        <property type="gene ID" value="Nwi_0036"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0036"/>
    <dbReference type="eggNOG" id="COG3807">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086360_0_0_5"/>
    <dbReference type="OMA" id="HRIDWVF"/>
    <dbReference type="OrthoDB" id="1857055at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010466">
        <property type="entry name" value="DUF1058"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06347">
        <property type="entry name" value="SH3_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229280" evidence="1">
        <location>
            <begin position="22"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="176" mass="19372" checksum="0BD9BAD58FD3D259" modified="2005-10-11" version="1">MTVRWRVLSMVFAAATLGAVAIETTADAKDSALSTSGLPVPRYVSLKSDHVNVRAGPTKDNDVAWVYTKAGLPVEITAEFENWRRIRDSEGAEGWVYHSLLSGRRTAVVTMKAKDDFTPLYDRADVQGNVAARLQAGVVTQVKRCAAGWCHVTGDGFDGWIEQQRLWGVYADEKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMU2</accession>
    <name>Q3SMU2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nitrogen-fixing NifU</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0009</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ABA03277.1"/>
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    <dbReference type="EnsemblBacteria" id="ABA03277">
        <property type="protein sequence ID" value="ABA03277"/>
        <property type="gene ID" value="Nwi_0009"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0009"/>
    <dbReference type="eggNOG" id="COG0694">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060555_0_2_5"/>
    <dbReference type="OMA" id="AIMEHYM"/>
    <dbReference type="OrthoDB" id="2010888at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.70">
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    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014824">
        <property type="entry name" value="Nfu/NifU_N"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="NFU1-like"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF08712">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HIRIP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00932">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SSF117916"/>
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    </dbReference>
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    <feature type="domain" description="Nfu_N" evidence="1">
        <location>
            <begin position="3"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="190" mass="20382" checksum="EE53E3DDB5EE332D" modified="2005-10-11" version="1">MFIQTEITPNPATLKFLPGRTVLGKGTMEFTSASTTARSPLAVKLFAVHGVAGVFYGSDFITVTKDEGSDWQHLKPAILGVIMEHYMAGAPLLADGEGCSEANADSDVEFFDEADAETVTLIKDLIETRVRPGVADDGGDITFRGFRDGIVYVNMKGACSGCPSSTVTLRNGIQNLLKHFVPEVVEVRPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMV4</accession>
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    </protein>
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    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06387">
        <property type="protein sequence ID" value="ABA06387"/>
        <property type="gene ID" value="Nwi_3140"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3140"/>
    <dbReference type="eggNOG" id="ENOG502ZR0B">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134429_1_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="156" mass="17251" checksum="60B28A2CC719A6D1" modified="2005-10-11" version="1">MCYNLVLALLASGRSSQTKALFCVVRNSILSCQAGGIMKQRISVFWSTCLVAFFAAWVPPGHAQPAVAGPAYSPPFTETLSNHVPLAFGMDPMTVANALGETLNYMGGSPGNEVFLALLKYGGSNLFNRKDRLYLQFRRGRLVGWKADWGRNWMWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMV7</accession>
    <name>Q3SMV7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_3137</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06384">
        <property type="protein sequence ID" value="ABA06384"/>
        <property type="gene ID" value="Nwi_3137"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3137"/>
    <dbReference type="HOGENOM" id="CLU_1064894_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="261" mass="29520" checksum="66309A8BCF020A6E" modified="2005-10-11" version="1">MRVMDIPSFPRPIEILDLETLFAARLNETVVMWPRQAIPLLGYMCHPNDEAERHGLVNLLRSWPNYEGPGQPPVPERLPRIQGNWLKVTDIFHLYCDLIDGQHQERRGGPSIGKAISLVEANAKSRGTSETNLWKLWSDYKDVAHLVTAATLVCAEVCTRFSESPPRLNPTQFLPFQMALLMPDLVLAVAQEFERLGRAKREDPHSEPALDADTHWRIPSDINVAPLPPPPRKIRPQDIKVLNDRRAGNRGRANKTTPVSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMX6</accession>
    <name>Q3SMX6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_3118</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316273.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06365">
        <property type="protein sequence ID" value="ABA06365"/>
        <property type="gene ID" value="Nwi_3118"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3118"/>
    <dbReference type="HOGENOM" id="CLU_2343849_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="97" mass="10855" checksum="5D11347BEE269D2D" modified="2005-10-11" version="1">MTKPSGWKHSPEAKAKIAERNRARWADPAERARVSEETKIRMADPAVRQRIRDGMARAAGVADALQPLRDAWRSAAPDVRKRFLEELFAPACGESSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMX7</accession>
    <name>Q3SMX7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_3117</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3117"/>
    <dbReference type="EnsemblBacteria" id="ABA06364">
        <property type="protein sequence ID" value="ABA06364"/>
        <property type="gene ID" value="Nwi_3117"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3117"/>
    <dbReference type="eggNOG" id="COG1725">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106278_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="197" mass="21544" checksum="A57F934E1B7A72DA" modified="2005-10-11" version="1">MRGVERMSIQAVAWALEQHINDPRSKLVLISLANHADHVTGRCWPSMATIGREASCRRETVLRHVKALEESGFIKVIRAAGGLRKRVHTYELCMSRCATDAHQTGAQSEALMCADRTSNCATHRTSMNHHRTITSPLPSSPPPPVENLSRSGNGKEATQRSAMHPASRSSVRSDGPEVIQNRIAAARSGLRPGERSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN04</accession>
    <name>Q3SN04_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_3090</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3090"/>
    <dbReference type="EnsemblBacteria" id="ABA06337">
        <property type="protein sequence ID" value="ABA06337"/>
        <property type="gene ID" value="Nwi_3090"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3090"/>
    <dbReference type="eggNOG" id="COG2020">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065200_1_2_5"/>
    <dbReference type="OMA" id="FWPAGTF"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007318">
        <property type="entry name" value="Phopholipid_MeTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04191">
        <property type="entry name" value="PEMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA06337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="232" mass="26024" checksum="4F79B4ECD7508009" modified="2005-10-11" version="1">MTVTEPPPMTRALAISYAIGLPLALLAIVFLPVGRLDWSPGWFFVTFLIVVYGISALVLWRVNPVIYRARSRFQPGTERWDRILLALMLPAMVAEIPVATFDAGRMSWSDVPFSIVVLGYVLLVAGIALGAWAQAVNRFFEPGVRIQRERGQHVITAGPYQFVRHPGYVSAIMIFVGVPFSLASWWALIPAAFATAILILRTSWEDALLRTELDGYADYARRTRFRLMPGIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN15</accession>
    <name>Q3SN15_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_3078</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06326">
        <property type="protein sequence ID" value="ABA06326"/>
        <property type="gene ID" value="Nwi_3078"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3078"/>
    <dbReference type="HOGENOM" id="CLU_2396675_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="93" mass="11075" checksum="4EDD8DB9AE1A4CEB" modified="2005-10-11" version="1">MRFFEAIAISDAFLKLFRATYPGLVYASLWQCNHIRLDIFKDWLDRPRRSSAHLPETPAATRSNDHSRRQRFDAQSFTHVSPRRGCEHWRDNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN32</accession>
    <name>Q3SN32_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">3-oxoacyl-[acyl-carrier-protein] reductase</fullName>
            <ecNumber evidence="3">1.1.1.100</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_3061</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.100" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316225.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3061"/>
    <dbReference type="EnsemblBacteria" id="ABA06309">
        <property type="protein sequence ID" value="ABA06309"/>
        <property type="gene ID" value="Nwi_3061"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3061"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_5"/>
    <dbReference type="OMA" id="MFEVNVY"/>
    <dbReference type="OrthoDB" id="1601931at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018454">
        <property type="term" value="F:acetoacetyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042619">
        <property type="term" value="P:poly-hydroxybutyrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011283">
        <property type="entry name" value="Acetoacetyl-CoA_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01829">
        <property type="entry name" value="AcAcCoA_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="241" mass="25233" checksum="88DD1E4B6F8AFE80" modified="2005-10-11" version="1">MARVALVTGGTRGIGGAISKALKAAGYNVAASYAGNDAAAEKFKAETGIPVHKWDVASFEACAGGIKQVEADLGPVEVLVNNAGITKDTAFHKMTFEQWSAVINTNLGSLFNMTRPLIEGMRARKFGRIINISSINGQKGQFGQVNYSAAKAGEIGFTKALALENAKTGITVNAICPGYINTEMVQAVPKDVIEKSILPQIPVTRLGEPEEIARAVVFLAADDAGFITGSTLSVNGGQYLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN80</accession>
    <name>Q3SN80_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_3011</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316181.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3011"/>
    <dbReference type="EnsemblBacteria" id="ABA06261">
        <property type="protein sequence ID" value="ABA06261"/>
        <property type="gene ID" value="Nwi_3011"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3011"/>
    <dbReference type="eggNOG" id="ENOG5030XRM">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2506175_0_0_5"/>
    <dbReference type="OMA" id="AVEWMEF"/>
    <dbReference type="OrthoDB" id="1922922at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="85" mass="9187" checksum="8B1827D849EE9F8D" modified="2005-10-11" version="1">MDKVSDSITKLQATFRITVNGESIAIATVGQAYDFITRLSTAEWAEFRTLHEDACAALEAAAGNAMLSRKATDALRALLARTKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNE6</accession>
    <name>Q3SNE6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Resolvase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2945</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316118.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2945"/>
    <dbReference type="EnsemblBacteria" id="ABA06195">
        <property type="protein sequence ID" value="ABA06195"/>
        <property type="gene ID" value="Nwi_2945"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2945"/>
    <dbReference type="eggNOG" id="COG1961">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010686_18_15_5"/>
    <dbReference type="OrthoDB" id="1791095at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038109">
        <property type="entry name" value="DNA_bind_recomb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011109">
        <property type="entry name" value="DNA_bind_recombinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07508">
        <property type="entry name" value="Recombinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="SSF53041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51737">
        <property type="entry name" value="RECOMBINASE_DNA_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="1">
        <location>
            <begin position="10"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Recombinase" evidence="2">
        <location>
            <begin position="170"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="550" mass="61519" checksum="FA49EFC94B7D431F" modified="2005-10-11" version="1">MKPDDNRVVRCAIYTRVSTDTGLDQEFNSLDAQYDASQAYIRSQAHAGWSLVKARYDDGGFSGGSTDRPALQRLLADVAARRIHIIVVYKVDRLTRSLADFAKLVELFDAHGVSFVSVTQQFNTTTSMGRLTLNVLLSFAQFEREVTSERIRDKIAASKRKGLWVGGMVPLGYILKDGQLHLQEEEANTVRLIFQRYLELGSVNRLVKDLKERGFKSKVRQLASGGTRGGVPFTQGPLFYMLRNRFYLGEVRFKGEILPGPQPALLDRALFEAVQKKLTEQRSHHTTTRIRTRALLAGLLFDDAGHRMILTYASRNRVRHRYYVSAPCIRGQTDEPVGSVTRVPASDIEASISKAVMANTDVDEPSSNETLSREVLERFVIQIEVRKNQLAIHLRSGENDIQGTQDDPDPDESRAVATRIIVVPWKKPSRPAREILLPANTPEHRARPMKAERRSALIRAIGRGRLWLQEIVDGRETIESLAARQHCSTRHIKMTISLAFLAPTLVTAAVEGRFPRGIGVAALRDAPAEWSQQMRRLGLAPTGTPGPASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNM8</accession>
    <name>Q3SNM8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2863</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2863"/>
    <dbReference type="EnsemblBacteria" id="ABA06113">
        <property type="protein sequence ID" value="ABA06113"/>
        <property type="gene ID" value="Nwi_2863"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2863"/>
    <dbReference type="eggNOG" id="COG0433">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008548_0_0_5"/>
    <dbReference type="OMA" id="FYGERIC"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="921" mass="101566" checksum="C05101C2C4D1CA16" modified="2005-10-11" version="1">MYRPPAAWSHRRRQFLNLGGRMSDQALDALSEAISEERPVILVAGQAFGASDTHGEAILNGFLDRLGRKAQTGGWRVALTDPVTDADMAWLSERFDRHVPSESTLRVFDLPWSAVFTSSIDPRFAGRFETRGRQPEAVLSRGAYARVSRSRSRPPVYYLFGRANETVSDARPPKTKADLLRRSQNHASELLNRLAETATARGLVVIAGFDPARDWLDVDALLATLSDQAGVRAIWFGAPQRLDSVVADEMIAQGSLITTQTTVAAALASLDLQGRINMVGAAAPDEPGIVTTSAGILDITPQLRLRVEASARIVDDAWTEHPEPLEVSEFHEAFRKFHGDLGGFRGMMEGVAREFAIQRSFETELWKAIEAAIRQPQESDSLVVLHGQSGTGKSVSLARLAWKLRRDLALPVLVAAGRLPTPTDVDAFCSAAERTGSPVTIVVCDANQSPWRYRDLAAALQSRGRRVVVVGSSYRIEEHSRQGFRRCVEAPATLSAAESRDLEKVVARFSSTQSASEASPPEGWNALAWLYRHLSAGRQRIAAGISSEARFAESAVRERARSAPRASGRSALADQLIAAGIAEESTQLFDNDEALAATGEDAAARLIDLVMVAGRLSCPVPLNLLMRVISDRSDGLGVEGVAHLFQDLDLFRWRAADAEGTELLISPRLQLEAELICQRRLADPSREIDCLIELIKGVRPSGVDRNIERTFVLDLLQKLDRDGPRGQAYRRGYLRFADALEDLRKKAGVRDASLMLRECVFRRQAIFSLDGYNSTEEMDEDARLMVLDAARAVVEEALRLIDLGELHAGKRNRVNLIVERASIYGYLAVQRTNSGGNALWADYLAARTASARATAMSDDYHPFDVALWTSCDVLRGATLSDEQRAELLSDLYSTMDLADGMDFRSDQRIRYLERRVRRQHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNQ4</accession>
    <name>Q3SNQ4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF3883 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2835</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316027.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2835"/>
    <dbReference type="EnsemblBacteria" id="ABA06087">
        <property type="protein sequence ID" value="ABA06087"/>
        <property type="gene ID" value="Nwi_2835"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2835"/>
    <dbReference type="eggNOG" id="COG3440">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061574_0_0_5"/>
    <dbReference type="OMA" id="NGWERTP"/>
    <dbReference type="OrthoDB" id="1475643at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024975">
        <property type="entry name" value="NOV_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13020">
        <property type="entry name" value="DUF3883"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3883" evidence="1">
        <location>
            <begin position="159"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="277" mass="31635" checksum="0A7B4709F4DE13DB" modified="2005-10-11" version="1">MSNGPWTDEENDLIVADYFAMLADDIAGRPYNKAEHDRQLQERIDRTRTSIEFKHQNISAVLKGLGEDWIPGYKPAFNFQMTLVDAVARWLAFNPDWLGQMPGTHPATGLQEAAQLWIGPPPTLSNQPPPQELEQMLHIARKFDVAGRDERNRSLGRAGEERVLAHERASLQAVGREDLARKVRWVSEEDGDGAGYDIESFAPDGRSRLIEVKTTNGWERTPFHITRNELAVAEERRSEWRLFRLWNFSREPKAFELHPPLDAHVSLTATTFQASFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNT4</accession>
    <name>Q3SNT4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphoesterase, PA-phosphatase related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2804</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316002.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2804"/>
    <dbReference type="EnsemblBacteria" id="ABA06057">
        <property type="protein sequence ID" value="ABA06057"/>
        <property type="gene ID" value="Nwi_2804"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2804"/>
    <dbReference type="eggNOG" id="COG0671">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070327_1_0_5"/>
    <dbReference type="OMA" id="CSFFSGE"/>
    <dbReference type="OrthoDB" id="1928533at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxi_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="SSF48317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="acidPPc" evidence="2">
        <location>
            <begin position="92"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="262" mass="28809" checksum="3C9AC8DD4B4ED5F6" modified="2005-10-11" version="1">MNRPGLFIALGLALVIGVVFGIHPEYDLRLAALFYDSATGTFPLKSDALASFARNAAMWVAWGFAVPAIVALAVKMFRPDRPLMMSGRAVAFLLITMLLSAGILTNLTFKSYWARPRPVMVTEFSGPWKFMPWWDPRGECGRNCSFFSGEGATAFWTLAPAALTPPAWRPLAYAGALAFGAVTSVLRMAFGGHFFTDVATAGLVAFLVIWLVHGYIYRWPSTRKSDAGIDAALTRFAWPGYRLRQRWRGRDVGPAPAPEKPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNY8</accession>
    <name>Q3SNY8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zinc-containing alcohol dehydrogenase superfamily</fullName>
            <ecNumber evidence="2">1.6.5.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2750</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.6.5.5" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315948.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2750"/>
    <dbReference type="EnsemblBacteria" id="ABA06003">
        <property type="protein sequence ID" value="ABA06003"/>
        <property type="gene ID" value="Nwi_2750"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2750"/>
    <dbReference type="eggNOG" id="COG0604">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_3_4_5"/>
    <dbReference type="OMA" id="VWSNVFM"/>
    <dbReference type="OrthoDB" id="1027479at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003960">
        <property type="term" value="F:NADPH:quinone reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd05276">
        <property type="entry name" value="p53_inducible_oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014189">
        <property type="entry name" value="Quinone_OxRdtase_PIG3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02824">
        <property type="entry name" value="quinone_pig3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PKS_ER" evidence="1">
        <location>
            <begin position="17"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="332" mass="34597" checksum="32AA9028A8ED3F52" modified="2005-10-11" version="1">MEKPPAQMTAIGISKPGGPEVLVPQLRPVPSPGPREVLIKVAAAGVNRPDVLQRSGSYPPPPGASDLPGLEVAGEVVALGEGASRYRPGDKVMSLVSGGGYAQYCVAHEAIAIPVPTAFSMIEAGGTPETLITVWHNVFERGALKAGETLLIHGGSSGIGTMAIQLAKVFGARTIVTVGSKEKAGACLGLGADHAINYKLQDFVAEVKAATAGAGADLILDMVGGDYIEKNYDAAALEGRIVQIAFLGGAKATVNLARLMVKRLHHTGSTLRPRSVADKAAMVAAIEAKVLPMMRERRIKPLIDSSFPLEQAADAHRRMETSAHVGKIVLTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP05</accession>
    <name>Q3SP05_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2733</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315931.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2733"/>
    <dbReference type="EnsemblBacteria" id="ABA05986">
        <property type="protein sequence ID" value="ABA05986"/>
        <property type="gene ID" value="Nwi_2733"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2733"/>
    <dbReference type="eggNOG" id="ENOG50334CR">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170379_1_0_5"/>
    <dbReference type="OMA" id="DMRIERQ"/>
    <dbReference type="OrthoDB" id="2034863at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025310">
        <property type="entry name" value="DUF4164"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13747">
        <property type="entry name" value="DUF4164"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="111" mass="11979" checksum="8780DC6048E7554B" modified="2005-10-11" version="1">MNDRPANRSASAEPPNGGGGTDIDAATRRLTAALDALERAVERRRDADRDEDELASRIQALGADRSRLADELDGSLVRTRKLERTNREIAEKLDTAIGAIKSVLEAAGEKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="133" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP35</accession>
    <name>Q3SP35_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Dihydroorotase multifunctional complex type</fullName>
            <ecNumber evidence="8">3.5.2.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_2703</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the reversible cyclization of carbamoyl aspartate to dihydroorotate.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallo-dependent hydrolases superfamily. DHOase family. Class I DHOase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.5" evidence="8"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315902.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2703"/>
    <dbReference type="EnsemblBacteria" id="ABA05956">
        <property type="protein sequence ID" value="ABA05956"/>
        <property type="gene ID" value="Nwi_2703"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2703"/>
    <dbReference type="eggNOG" id="COG0044">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015572_1_1_5"/>
    <dbReference type="OMA" id="WVTAEVT"/>
    <dbReference type="OrthoDB" id="906155at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004038">
        <property type="term" value="F:allantoinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002195">
        <property type="entry name" value="Dihydroorotase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00483">
        <property type="entry name" value="DIHYDROOROTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="7">
        <location>
            <begin position="54"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA05956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="442" mass="48424" checksum="0FF115045AF421F3" modified="2005-10-11" version="1">MTKTFDVILRSGAIVNQDGEGVADIGVSGGRIAAIGGLGQADANEVIDCRGLHILPGVIDTQVHFREPGLTHKEDLETGSRSAVMGGVTAVFEMPNTNPLTVTAETFAAKMKAAHHRMHCDFAFFIGGTRENVRELPELERAPGCAGVKVFVGSSTGALLVEDDDSLRRIFNVIRRRAAFHAEDEYRLNERKSLRVENDPRSHPLWRDETAALLATQRLVTLARETGKRIHVLHISTREEIEYLRDHKDVASCEATPHHLTMAAPECYERLGTLAQMNPPVRDAAHRSGIWKGIEQGIIDVLGSDHAPHTLEEKRKTYPASPSGMTGVQTLVPLMLDHVSSGRLSLARFVDLTSAGPARLFNIACKGRVAAGFDADFTVVDLKRTETITNKWVASRAGWTPYDGVEVTGWPVGTFVRGRRVMWQGELATASTGEPVRFLETL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP63</accession>
    <name>Q3SP63_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glutathione S-transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2675</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315874.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2675"/>
    <dbReference type="EnsemblBacteria" id="ABA05928">
        <property type="protein sequence ID" value="ABA05928"/>
        <property type="gene ID" value="Nwi_2675"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2675"/>
    <dbReference type="eggNOG" id="COG0435">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037263_1_0_5"/>
    <dbReference type="OMA" id="YQLFVSY"/>
    <dbReference type="OrthoDB" id="780709at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016639">
        <property type="entry name" value="GST_Omega/GSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32419">
        <property type="entry name" value="PTHR32419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13409">
        <property type="entry name" value="GST_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015753">
        <property type="entry name" value="GST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01206">
        <property type="entry name" value="Xi.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="SSF47616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST C-terminal" evidence="2">
        <location>
            <begin position="143"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR015753-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="316" mass="35390" checksum="77D44401DA1AA3AF" modified="2005-10-11" version="1">MNGKWTSDWQPVQAKDEKGGFVRQTSSFRNWITPDGSAGPSGESGFPAERGRYHLYVALICPWASRTLIARKLKKLEEVVSASVVEPVLTDQGWRFGDYPGAGRDEVNGATYLHELYTRADPEICGRATVPVLWDKKRKTIVNNESAEIVRMFNSGFAGLADDSVDLYPQGLRSAIDALNDRIYPALNNGVYRAGFATTQIAYDEAFDDVFAMLDLLENYLANRPFLLGPRFTEADIRLFVTLVRFDTAYHGLFKCNLRRLSEYPSLTAYLKRVLDVPGIRETVSIDHIKRGYYSIKSLNPNGIVPKGPDLSHLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPE7</accession>
    <name>Q3SPE7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF2007 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2591</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2591"/>
    <dbReference type="EnsemblBacteria" id="ABA05844">
        <property type="protein sequence ID" value="ABA05844"/>
        <property type="gene ID" value="Nwi_2591"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2591"/>
    <dbReference type="eggNOG" id="ENOG5032YJ8">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_171578_0_1_5"/>
    <dbReference type="OMA" id="LDQNMSI"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018551">
        <property type="entry name" value="DUF2007"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09413">
        <property type="entry name" value="DUF2007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2007" evidence="2">
        <location>
            <begin position="4"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="84" mass="9044" checksum="62E391210975CD17" modified="2005-10-11" version="1">MTLQELVRTNDMVLVSAVGALLDGAGIFHLVLDQNMSVLEGSLGVLPRRVLVNDGDNRAARRLLAEAGLAHELRPDDADDLRAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPG7</accession>
    <name>Q3SPG7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein phosphatase CheZ</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Chemotaxis protein CheZ</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_2571</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CheZ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315775.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2571"/>
    <dbReference type="EnsemblBacteria" id="ABA05824">
        <property type="protein sequence ID" value="ABA05824"/>
        <property type="gene ID" value="Nwi_2571"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2571"/>
    <dbReference type="eggNOG" id="COG3143">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053644_1_0_5"/>
    <dbReference type="OMA" id="RAQIEQC"/>
    <dbReference type="OrthoDB" id="1206193at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009288">
        <property type="term" value="C:bacterial-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097588">
        <property type="term" value="P:archaeal or bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050920">
        <property type="term" value="P:regulation of chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007439">
        <property type="entry name" value="Chemotax_Pase_CheZ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04344">
        <property type="entry name" value="CheZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0283">Flagellar rotation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="45"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="69"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="53"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="259" mass="28352" checksum="B016BE18DCC1B75A" modified="2005-10-11" version="1">MAVQRKRFRVEEILSGGTPAIATLDEAPMPVHREIMAELRAIRQQMGAPHRSDPKQSDADNEPDAGREATDAQALLQTYRTQIEQCEKLKTELDLIYEAINRTKQEIAVLHGKSFDGNEMAKVTDELDAVVGGTEQATQRILEAAEAIDQSAGALSGAASPEQARLLSEDIQERVISIFEACNFQDLTGQRIAKVMSTMTFIEHHINVMMDIWGGVDAIRAHAPPVIDNRVGDAKLLNGPKLEGDEGHASQDDIDALFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPI4</accession>
    <name>Q3SPI4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2554</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2554"/>
    <dbReference type="EnsemblBacteria" id="ABA05807">
        <property type="protein sequence ID" value="ABA05807"/>
        <property type="gene ID" value="Nwi_2554"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2554"/>
    <dbReference type="eggNOG" id="COG3908">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2058909_0_0_5"/>
    <dbReference type="OMA" id="RVEQPWR"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018661">
        <property type="entry name" value="DUF2093"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09866">
        <property type="entry name" value="DUF2093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="119" mass="13641" checksum="9937169D372CC8BA" modified="2005-10-11" version="1">MMRSRSERRPDGVSSSRWFNHSVFERSGIRSASRKRVEQPWRKAVLNKFRQSGQGEASAQYLDGDFRVISPGTYVRCAVTDARIPLDELKYWSVDLQEAYALPSAVLQRHYPGAFKSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPV8</accession>
    <name>Q3SPV8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Diguanylate cyclase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2429</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315641.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2429"/>
    <dbReference type="EnsemblBacteria" id="ABA05683">
        <property type="protein sequence ID" value="ABA05683"/>
        <property type="gene ID" value="Nwi_2429"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2429"/>
    <dbReference type="eggNOG" id="COG3706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_11_1_5"/>
    <dbReference type="OMA" id="ARYWTAS"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="2">
        <location>
            <begin position="252"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="406" mass="43933" checksum="D6E16EE0D79F197D" modified="2005-10-11" version="1">MLSVPTIWVVFIANFLALGLVWTYVMRSYPTLVAAPYWAAASYFAALFSALSMLRGHVDSMLPLVIGGGGLIFSICLCTMGIRRFYERPVSWRTTFLTVALSVAGLAYFVYVRDDMPMRIFIYSAAQALPIAMTLKMVLSRKAGRINSGAQLAGIVSVLIICVSVIRSVCAFFDIGGGVSYINFNGLQAALILGLVFLSMTWNFGFLLMAIDRLRNEVADLALVDDLTGVANRRQLLSRLEDACAMSERSGEPFVILMMDLDGFKQINDTHGHAAGDACLRHFTLMAQMQLRPGDLLARTGGDEFCILLPATTLQQGAQIAQRVLNACREDAAGCAGADVPISASIGLAQWTQEMGACSEHLLAAADHALYAAKNDGKNRFASCGDIAPPIAPQSWPQQIPSDQRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPW5</accession>
    <name>Q3SPW5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stress-response A/B barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2422</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2422"/>
    <dbReference type="EnsemblBacteria" id="ABA05676">
        <property type="protein sequence ID" value="ABA05676"/>
        <property type="gene ID" value="Nwi_2422"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2422"/>
    <dbReference type="eggNOG" id="ENOG5032T7W">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080664_7_0_5"/>
    <dbReference type="OMA" id="HIVFFTV"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013097">
        <property type="entry name" value="Dabb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07876">
        <property type="entry name" value="Dabb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00886">
        <property type="entry name" value="Dabb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51502">
        <property type="entry name" value="S_R_A_B_BARREL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Stress-response A/B barrel" evidence="1">
        <location>
            <begin position="14"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="109" mass="12278" checksum="74C4F3204C52777D" modified="2005-10-11" version="1">MRLGSPSVPRPAMIRHIVLFTAKDKGDIDKIIDGLSPLTTIPHARRLEVARNRKTDQLGNDIDVVVYGEFDSEVELAAYKAHDLYQASTKRVQPLRDMRFAADYDVPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPW9</accession>
    <name>Q3SPW9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2418</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315630.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2418"/>
    <dbReference type="EnsemblBacteria" id="ABA05672">
        <property type="protein sequence ID" value="ABA05672"/>
        <property type="gene ID" value="Nwi_2418"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2418"/>
    <dbReference type="eggNOG" id="ENOG50317EK">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1685241_0_0_5"/>
    <dbReference type="OMA" id="WIGGVSM"/>
    <dbReference type="OrthoDB" id="1736268at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="159" mass="18064" checksum="D91FEDFD3922120F" modified="2005-10-11" version="1">MDDLIVARALHVLAVVVWIGGVSMATTVALPALRRGDLGPDRLKAFEAIERRFIWQARASLIIVGLSGLYLTWQLDLWDRFRMPAFWWMDAMVGLWLLFAFVLFVGEPLILRRTFHRLATEQPDIAFAWLHRAHWVLLGLGLATVFAAVAGSHGWLIFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ00</accession>
    <name>Q3SQ00_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NAD-dependent epimerase/dehydratase</fullName>
            <ecNumber evidence="3">5.1.3.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2387</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.2" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315602.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2387"/>
    <dbReference type="EnsemblBacteria" id="ABA05641">
        <property type="protein sequence ID" value="ABA05641"/>
        <property type="gene ID" value="Nwi_2387"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2387"/>
    <dbReference type="eggNOG" id="COG0451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_6_1_5"/>
    <dbReference type="OMA" id="PLGAIHN"/>
    <dbReference type="OrthoDB" id="1928091at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003978">
        <property type="term" value="F:UDP-glucose 4-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="2">
        <location>
            <begin position="10"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="321" mass="33877" checksum="BE0CA8A7A570BA1B" modified="2005-10-11" version="1">MITPPHRTALLTGITGFLGPYVAAALRADGWSVRAALRRPIGDLPACDGSVLVGDLGGATDWTDALVGIDAVVHLAGRAHRPPAVQLAEQDLYFETNTAGTIHLAQAAAAAGVRNFVFISSIAVNGVSTDGRAPFRESDAPAPQSIYGRTKAAAEDGLGEVAVKTGMAVTAIRPPMIYGANAKGSFRILSRAVTAHVPLPFGMIRNRRAFVAAENVASFISFRLERPGHGMETYIVSDEEQISTAEFSRRIGRALGRNVLLLPVPEKALGFVLRVAGATHLIESILHSLVVDTGKIDRSGWRPAVSLDQGLLLALGKTPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ01</accession>
    <name>Q3SQ01_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase, group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2386</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315601.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2386"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05640">
        <property type="protein sequence ID" value="ABA05640"/>
        <property type="gene ID" value="Nwi_2386"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2386"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_11_0_5"/>
    <dbReference type="OMA" id="WMAREGH"/>
    <dbReference type="OrthoDB" id="1591999at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13579">
        <property type="entry name" value="Glyco_trans_4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="2">
        <location>
            <begin position="21"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="399" mass="44136" checksum="14B01B27B4A9143A" modified="2005-10-11" version="1">MTAFLVTQHFRPDETSTSVFLTAIAEELAKDDDVVVISGTRGSARAGNPAVLEIPSWSPPKTALIRRALAMLWFCIGVLAALTWRARRDTPVFVVTTPFMLPYFATLAAWLRGAPATLILYDLYPESLIAANLIAKESWTARLILRLNEWMFHILKAVVVIGRDMENHLSRYKGATPDKICFIPHWSTLAPGLRPVDPDNRFRPAGTDKLVVGLSGNLGFTHDPNTVFAAARALADQPDVHFLLSGWGVGWHRLVDAQRAAALPNVTLVERVAETDLENFLTAADVWIIPYRRGMSGISSPSRLYNLLATGRPVIALSESDAEYGLMLSEHRAGWVVEPENAAELAQTIREAARNPEDVAVRSRNAAQLLQDRFTRDSAGDAYRRLANRLRKARKTPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ02</accession>
    <name>Q3SQ02_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">GDP-mannose 4,6-dehydratase</fullName>
            <ecNumber evidence="3 4">4.2.1.47</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">GDP-D-mannose dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">gmd</name>
        <name evidence="6" type="ordered locus">Nwi_2385</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the conversion of GDP-D-mannose to GDP-4-dehydro-6-deoxy-D-mannose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>GDP-alpha-D-mannose = GDP-4-dehydro-alpha-D-rhamnose + H2O</text>
            <dbReference type="Rhea" id="RHEA:23820"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:57964"/>
            <dbReference type="EC" id="4.2.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the NAD(P)-dependent epimerase/dehydratase family. GDP-mannose 4,6-dehydratase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.47" evidence="3 4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315600.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2385"/>
    <dbReference type="EnsemblBacteria" id="ABA05639">
        <property type="protein sequence ID" value="ABA05639"/>
        <property type="gene ID" value="Nwi_2385"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2385"/>
    <dbReference type="eggNOG" id="COG1089">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_14_1_5"/>
    <dbReference type="OMA" id="FVKSSWQ"/>
    <dbReference type="OrthoDB" id="955232at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008446">
        <property type="term" value="F:GDP-mannose 4,6-dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070401">
        <property type="term" value="F:NADP+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019673">
        <property type="term" value="P:GDP-mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00955">
        <property type="entry name" value="GDP_Man_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006368">
        <property type="entry name" value="GDP_Man_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43715">
        <property type="entry name" value="PTHR43715"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01472">
        <property type="entry name" value="gmd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="5">
        <location>
            <begin position="12"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="361" mass="40390" checksum="4672602A88B49227" modified="2005-10-11" version="1">MSAGGATRRVALITGVTGQDGAYLAEYLLGLGYIVHGIKRRSSSFNTARIDHLYEDPHKGDVRFLLHYGDMTDSTNLIRLMQQIRPTEIYNLAAQSHVAVSFESPEYTANADAIGVLRLLEAIRILGMEKETRFYQASTSELYGLVQEVPQKETTPFYPRSPYAVAKLYGYWITVNYREAYGMYASNGILFNHESPIRGETFVTRKVTRSVARIEAGLEQCLYLGNLDAKRDWGHARDYVEGMHLILQADGPDDFVLATGETRAVREFVEIAFQEVGRRIEWRGKGVEEVGVDIKSGQIVVRIDPQYFRPTEVDLLVGDAAKARENLGWRPKTSFATLVREMVAADLAIAKREAVGGQNAV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ46</accession>
    <name>ILVC_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ketol-acid reductoisomerase (NADP(+))</fullName>
            <shortName evidence="1">KARI</shortName>
            <ecNumber evidence="1">1.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Acetohydroxy-acid isomeroreductase</fullName>
            <shortName evidence="1">AHIR</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Alpha-keto-beta-hydroxylacyl reductoisomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Ketol-acid reductoisomerase type 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Ketol-acid reductoisomerase type I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ilvC</name>
        <name type="ordered locus">Nwi_2341</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (2S)-2-acetolactate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:22068"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-ethyl-2-hydroxy-3-oxobutanoate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:13493"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49256"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ketol-acid reductoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.86" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315559.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SQ46"/>
    <dbReference type="SMR" id="Q3SQ46"/>
    <dbReference type="STRING" id="323098.Nwi_2341"/>
    <dbReference type="EnsemblBacteria" id="ABA05595">
        <property type="protein sequence ID" value="ABA05595"/>
        <property type="gene ID" value="Nwi_2341"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2341"/>
    <dbReference type="eggNOG" id="COG0059">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033821_0_1_5"/>
    <dbReference type="OMA" id="RAMFSWL"/>
    <dbReference type="OrthoDB" id="188901at2"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00056"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00060"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004455">
        <property type="term" value="F:ketol-acid reductoisomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00435">
        <property type="entry name" value="IlvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013023">
        <property type="entry name" value="KARI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000506">
        <property type="entry name" value="KARI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013116">
        <property type="entry name" value="KARI_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014359">
        <property type="entry name" value="KARI_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21371">
        <property type="entry name" value="PTHR21371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01450">
        <property type="entry name" value="IlvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07991">
        <property type="entry name" value="IlvN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000116">
        <property type="entry name" value="IlvC_gammaproteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00465">
        <property type="entry name" value="ilvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51851">
        <property type="entry name" value="KARI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51850">
        <property type="entry name" value="KARI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000226184" description="Ketol-acid reductoisomerase (NADP(+))">
        <location>
            <begin position="1"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="KARI N-terminal Rossmann" evidence="2">
        <location>
            <begin position="1"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="KARI C-terminal knotted" evidence="3">
        <location>
            <begin position="183"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="24"/>
            <end position="27"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="83"/>
            <end position="86"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01198"/>
        </source>
    </evidence>
    <sequence length="339" mass="37044" checksum="5EA0045CF435648F" modified="2005-10-11" version="1">MRVYYDRDADLNLIKGKKVVIVGYGSQGHAHALNLKDSGVKEIAIALRKGSASAKKAEGAGFKVMEVAEAAKWGDLVMMLTPDELQGDIYREHLHDNMKKGAALIFAHGLNVHFNLLDPRADLDVLMIAPKGPGHTVRAEYQRGGGVPCLIAVAKDSSGNAHDLGLSYASAIGGGRAGIIETTFREECETDLFGEQAVLCGGLVELIKNGFETLVEAGYAPEMAYFECLHEVKLIVDLIYEGGIANMNYSISNTAEYGEYVTGPRIVTAETKKEMKRVLDDIQSGKFARDWMLENKVNQTSFKATRARLAAHPIEEVGARLRDMMPWIKKAAMVDKAKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQB1</accession>
    <name>Q3SQB1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Fe-S oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2276</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2276"/>
    <dbReference type="EnsemblBacteria" id="ABA05530">
        <property type="protein sequence ID" value="ABA05530"/>
        <property type="gene ID" value="Nwi_2276"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2276"/>
    <dbReference type="eggNOG" id="COG1032">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021572_5_0_5"/>
    <dbReference type="OMA" id="IVIWRQG"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025274">
        <property type="entry name" value="DUF4070"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034530">
        <property type="entry name" value="HpnP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034466">
        <property type="entry name" value="Methyltransferase_Class_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13282">
        <property type="entry name" value="DUF4070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00303">
        <property type="entry name" value="hopanoid_C2-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01123">
        <property type="entry name" value="methyltransferase_(Class_B)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="6">
        <location>
            <begin position="173"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="553" mass="63335" checksum="F9B6072288D7EC1F" modified="2005-10-11" version="1">MRGVGQHGIRRILCVFPRYTSSFGTFEHAYPLTGGVQAFMPPQGLLLIAAYLPADWKVRFIDENIRFATDEDFEWAEAVFVSGMHIQRQQMNDICRRAHAFNLSVALGGPSVSACPDYYPSFDYLHVGELGDATDELIERLARDPSRPAAQVVLKTASRLDMDKFPIPAYELADIKHYFLGSIQYSSGCPYQCEFCDIPGLYGRNPRLKTPEQITRELDKLLECGISGSVYFVDDNFIGNRKAALELLPHLIEWQKKTGYVLRFSCEATLNIAKRPEILSQMREAFFATIFCGIETPDPVALKAMHKDHNMMVPIMEAIRTLNDYGMEVVSGIILGLDTDKPETGQHLLEFIDHSRIPLLTINLLQALPKTPLWDRLKRENRLIDDDGRDSNVDFRLPYDHVVATWRDCMGRAYEPEKLMERYEYQIRETYPKRIQPRTPQQRSWRNIKLGLTMLRNIFWKVGVRGDYRRVFWKFALRRLARGEIEYLISSVLVAHHLIMFARSASRGKTNASYYSLKLRETAETAVSAEHKAVATVPTLRADRSSGQRVTPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQL2</accession>
    <name>Q3SQL2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2175</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315398.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2175"/>
    <dbReference type="EnsemblBacteria" id="ABA05429">
        <property type="protein sequence ID" value="ABA05429"/>
        <property type="gene ID" value="Nwi_2175"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2175"/>
    <dbReference type="eggNOG" id="ENOG5032Z6W">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_184505_0_0_5"/>
    <dbReference type="OMA" id="FMFEMAI"/>
    <dbReference type="OrthoDB" id="2060959at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021473">
        <property type="entry name" value="DUF3126"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11324">
        <property type="entry name" value="DUF3126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="72" mass="8294" checksum="C6EEFF2EFC2A2800" modified="2005-10-11" version="1">MNVQEVRKLDAYLKRVFGNAKIRVVPRPKKDDSAEVYIGEEFIGVLFVDDEDDDRSFQFQMAILEEDLADVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQM1</accession>
    <name>Q3SQM1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2166</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2166"/>
    <dbReference type="EnsemblBacteria" id="ABA05420">
        <property type="protein sequence ID" value="ABA05420"/>
        <property type="gene ID" value="Nwi_2166"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2166"/>
    <dbReference type="eggNOG" id="COG5447">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_146719_1_0_5"/>
    <dbReference type="OMA" id="DPDLWIV"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009964">
        <property type="entry name" value="DUF1491"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07372">
        <property type="entry name" value="DUF1491"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="131" mass="14886" checksum="E233719FC46D047E" modified="2005-10-11" version="1">MRQLHWSRRHEARIFMSMRLKSSIWVAAYLRRCQCEGVFGAVRRRGADEAGAVFVKLALLDGNAMLYAPAPQAVYDDSRPVERVFAPSSVEPVAEQAVEERLAKEVRFDPDAWIVEIEDKAGRHFLDLARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQR4</accession>
    <name>Q3SQR4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2122</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315348.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2122"/>
    <dbReference type="EnsemblBacteria" id="ABA05377">
        <property type="protein sequence ID" value="ABA05377"/>
        <property type="gene ID" value="Nwi_2122"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2122"/>
    <dbReference type="eggNOG" id="COG3616">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119528_1_0_5"/>
    <dbReference type="OMA" id="MIPLATE"/>
    <dbReference type="OrthoDB" id="1558145at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021341">
        <property type="entry name" value="DUF2958"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11171">
        <property type="entry name" value="DUF2958"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="123" mass="13389" checksum="6E5820BF6E420C15" modified="2005-10-11" version="1">MILLTDDLRERLLANGRDRGADHVPVVKFFNPLGQGIWLASELDADGDTLFGLADLGFPELGSFSLEELASIVLPFGMGIERDILFTGDFPISVWAEAAREAGSIRAAERILYRAASPRRDGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQW5</accession>
    <name>Q3SQW5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nitrate reductase (quinone)</fullName>
            <ecNumber evidence="7">1.7.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Nwi_2068</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a quinol + nitrate = a quinone + H2O + nitrite</text>
            <dbReference type="Rhea" id="RHEA:56144"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16301"/>
            <dbReference type="ChEBI" id="CHEBI:17632"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.7.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.7.5.1" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315305.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2068"/>
    <dbReference type="EnsemblBacteria" id="ABA05326">
        <property type="protein sequence ID" value="ABA05326"/>
        <property type="gene ID" value="Nwi_2068"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2068"/>
    <dbReference type="eggNOG" id="COG5013">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_14_1_5"/>
    <dbReference type="OMA" id="GGHHNSV"/>
    <dbReference type="OrthoDB" id="88184at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009325">
        <property type="term" value="C:nitrate reductase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008940">
        <property type="term" value="F:nitrate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042126">
        <property type="term" value="P:nitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02776">
        <property type="entry name" value="MopB_CT_Nitrate-R-NarG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037943">
        <property type="entry name" value="MopB_CT_Nitrate-R-NarG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006655">
        <property type="entry name" value="Mopterin_OxRdtase_prok_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027467">
        <property type="entry name" value="MopterinOxRdtase_cofactor_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006468">
        <property type="entry name" value="NarG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00926">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01580">
        <property type="entry name" value="narG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00551">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00932">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 12" id="KW-0408">Iron</keyword>
    <keyword evidence="11 12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="13">
        <location>
            <begin position="26"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00010312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABA05326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1214" mass="138545" checksum="4AC9921243E92918" modified="2005-10-11" version="1">MSWILDLVNPRERKWEEFYRNRWSHDNVFRSTHGVNCTGGCSWAIYVKDGIITWEMQQTDYPLLERSLPPYEPRGCQRGISASWYVYSPIRVKYPYIRGPLADLWHEAKASYPDPVQAWASLVEDEEKRNRIQKARGKGGFRRAKWEELIELIAASCLYTARKHGPDRVMGFSPIPAMSMLSFAAGTRFLSLFGGGLMSFYDWYADLPTSFPEIWGDQTDVCESADWYNSKFIVSMASNMNMTRTPDVHFISEARTEGTKFVVLSPDFSQIAKYCDEWIPIQAGQDTALWMAANHVILKEYYIDRQVPYFIDYVKRYTDLPFLVELEPNGNTYKTGRLLRSNRVARYKDVENGEWKMLVLDTATGEPRAFKGQVGDRWGSTHGKWNLSAEDTLDNSPIDPVLSFIDQSDGVVQVGFDDFVNGSVVSRGVPVKRIATDKGEVLVTTGFDIMMSQFGHSRGLEGSFATSYDDEDAPYTPAWQERHTGIGRETAIRFAREFATNAELTNGKSMVIVGASANHWYYNNLCYRSATVALILCGCCGVNGGGINHYVGQEKLAPVAPWSTVAMALDWNKPPRVVQSSTWHYAHSCQWRYEQEFTEYGLTAPNPRWAKGHAIDLEAKSVRCGWMPFTPNFHRNPIEVVAEAERAGAKSTADIATYVADQVASKKLDLAINDPDAEENWPRVWFIWRANAIQSSAKGHEFFLRHYLGAHDNVIAEERAKGKTTTVKYRDTAPQGKYDLVVDINFRMNTTGLYSDIILPTAFWYEKNDLNTTDLHSFLHVLGQAVPPVWESKTDWEIFKLIAKKVSELSPLAFSKPVRDIVLQPLMHDTPDELAQPEILDWAEGECKLVPGKSFPHVRVVERDYANLYNKFISFGPKAREDGISAVGVNIPIKKQYDQMLENPIMPMPDSRHMRCVEWGGKRYPSLEDVLDACNVVLLCAPVANGEVSYQGFVNEEQHVGLPLADIAEPTRGVSSTFYDLTRQPRRILTSPCWTGLVNDGRAYSAWCMNIERLVPWRTLTGRQSLYLDHQWYLDFGEHIPTYKPRLNPRKTGDIVKSRVDDRSLVLNYITPHGKWNIHSTYKDNHRLLMLSRGMDPVWINDRDAEKVGIEDNDWVEVYNDNGVVVTRANVSRRIQPGTCMYYHAVERTVYIPKSQERKWRGGGHNSLTRIRINPLFLAGGYAQFTYGWNYWGPTGIFTRDTHVVVRKMEKVEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQW7</accession>
    <name>Q3SQW7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2066</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05324">
        <property type="protein sequence ID" value="ABA05324"/>
        <property type="gene ID" value="Nwi_2066"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2066"/>
    <dbReference type="HOGENOM" id="CLU_1738595_0_0_5"/>
    <dbReference type="OrthoDB" id="1864858at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="150" mass="15982" checksum="727BD83C7109EC93" modified="2005-10-11" version="1">MFINAAVLISTLFPAGRGRESLQGQPAHDLRWLQGYQRGGCLTIASNGAFQSGTKFITWRGALLVCAGRAGCRPNLRGAITGEVPNGRLGWRVYFSFEDIDESWIGSVRRASVPFKDQMGSSVTASRGSPGTTGARALVLSGHEPVMPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQX2</accession>
    <name>Q3SQX2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, Crp/Fnr family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2061</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315298.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2061"/>
    <dbReference type="EnsemblBacteria" id="ABA05319">
        <property type="protein sequence ID" value="ABA05319"/>
        <property type="gene ID" value="Nwi_2061"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2061"/>
    <dbReference type="eggNOG" id="COG0664">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075053_0_1_5"/>
    <dbReference type="OMA" id="IENNRHV"/>
    <dbReference type="OrthoDB" id="1587662at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012318">
        <property type="entry name" value="HTH_CRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018335">
        <property type="entry name" value="Tscrpt_reg_HTH_Crp-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13545">
        <property type="entry name" value="HTH_Crp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00034">
        <property type="entry name" value="HTHCRP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00419">
        <property type="entry name" value="HTH_CRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="SSF51206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00042">
        <property type="entry name" value="HTH_CRP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51063">
        <property type="entry name" value="HTH_CRP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH crp-type" evidence="2">
        <location>
            <begin position="158"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="241" mass="26802" checksum="75C5815336DC4551" modified="2005-10-11" version="1">MSTSRKDIHNSDVPKLCQSCEVRHKGMCGALNANELVDFAKHTRVVKVSAGDVLLQEQGSIRAYANVMRGVLKLTKTMADGRQQIVGLQFAPDFVGRLHQDESPVRVEACSGVEVCSITRNALRKMLEKNPALETKLLHQALREVDQGREWMLALGRKTAREKVASFLMMMVRQIDPFSRGNAATAFDLPLTRAEMGDFLGLTIGTVSRELTRLRRVGVIRISSHRHIDVLNLEALRQSAG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-11-03" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR42</accession>
    <name>DABA1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable inorganic carbon transporter subunit DabA 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dabA1</name>
        <name type="ordered locus">Nwi_1990</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of an energy-coupled inorganic carbon pump.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a complex with DabB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the inorganic carbon transporter (TC 9.A.2) DabA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315236.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SR42"/>
    <dbReference type="STRING" id="323098.Nwi_1990"/>
    <dbReference type="EnsemblBacteria" id="ABA05249">
        <property type="protein sequence ID" value="ABA05249"/>
        <property type="gene ID" value="Nwi_1990"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1990"/>
    <dbReference type="eggNOG" id="COG3002">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009885_0_0_5"/>
    <dbReference type="OMA" id="FWPMRTF"/>
    <dbReference type="OrthoDB" id="179501at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01871">
        <property type="entry name" value="DabA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018752">
        <property type="entry name" value="DabA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38344">
        <property type="entry name" value="PTHR38344"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10070">
        <property type="entry name" value="MpsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000387281" description="Probable inorganic carbon transporter subunit DabA 1">
        <location>
            <begin position="1"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="460"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="462"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="719"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="734"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01871"/>
        </source>
    </evidence>
    <sequence length="1037" mass="116947" checksum="0EA606DCEFDAAD15" modified="2005-10-11" version="1">MVDALNLGRRLRVRSTAYVAGEPVPFFWPMRTFIHHNPLYGLEDMPFEQAVRRGAELFHARMFLPRSDYQRWQREGKVRPETLKEEIGRRSQDLPPVPGVDWPRWLHALMQTPHDRDAVVRGVRAKDVHAALHGHPPSAQAVDVAALLPELEQRLHARTLPEAVDALWGTGLADELDELVIKNCLDFFDEDQSAWRMPGRERGLFSAWSEVTRRNARMVLRRLHVRHILDHVQDAESAVVYVMEEMGIGAEAWPTYFTRVLTRLHGWTGFVRWRASAKHYYWAQQYPADIVDLLAIRLVMGLALLQESARHRGTPMRRDDLGAVVRERGAECVLRYALHSGEVLPDWAQRIDDTLSRGNGARCHDLLQRYWPLWQARLGQQQAAALRELAAAANATAALDALAPEDVEGLLQGLRDFAPQEGMVWTLAMEGQAIDKLLTQVQAPQDPPPDKIPFAQAWFCIDVRAEPIRRHLERVGNYQTFGIAGFFGVPVGFLGYGKGSESHYCPAVITPKNLVLELPAALDPHNEDFVSTLGHVLHDLKKSVLSPYVTVEAVGMLFGLDLFGKTLAPLGYSRWRRRIDTEKPVTRLLVDKLSREQADSIIRTLQRAMIVKALHTELKIERERVDDDMIRELREIALRRRDGPTRLRTTFGVPQTQEAEFIDKLRQVYGVDADYTNHQLERLGRIGYSLDEQVNYVHTALTMIGLTQTFSRFVLVVGHGGKTENNPYESALDCGACGGASGIVNARVFAQMANKPAVRERLAAMGITIPEDTWFMPALHVTTTDAIELFDLDLLPPRHLVYLERLRDGLRAASRLTAAERMPKLLPEAKALEPAEALRLANRLAVDWAQVRPEWGLSGNVYGIVGRRALTENSDLQGSAFLLSYDWRCDPRGRLLENLLAAPVVVGQWINLEHFFSTVDNAHLGSGSKVYHNVSGRFGVMTGSLSDLRTGLPMQTVMREGRPYHEPMRLIALIEAPLDFAGRVLERVVKVKSLVLGGWIRAIVIDPTQGYKPFVFNNGQWEERTPLIAPAEKEYSA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-03" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR45</accession>
    <name>RBL1A_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribulose bisphosphate carboxylase large chain 1</fullName>
            <shortName evidence="1">RuBisCO large subunit 1</shortName>
            <ecNumber evidence="1">4.1.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cbbL1</name>
        <name type="ordered locus">Nwi_1987</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 (2R)-3-phosphoglycerate + 2 H(+) = CO2 + D-ribulose 1,5-bisphosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:23124"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57870"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="EC" id="4.1.1.39"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-ribulose 1,5-bisphosphate + O2 = (2R)-3-phosphoglycerate + 2-phosphoglycolate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:36631"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57870"/>
            <dbReference type="ChEBI" id="CHEBI:58033"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterohexadecamer of 8 large chains and 8 small chains.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">The basic functional RuBisCO is composed of a large chain homodimer in a 'head-to-tail' conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric 'cap' on each end of the 'barrel'.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RuBisCO large chain family. Type I subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.39" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315233.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SR45"/>
    <dbReference type="SMR" id="Q3SR45"/>
    <dbReference type="STRING" id="323098.Nwi_1987"/>
    <dbReference type="EnsemblBacteria" id="ABA05246">
        <property type="protein sequence ID" value="ABA05246"/>
        <property type="gene ID" value="Nwi_1987"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1987"/>
    <dbReference type="eggNOG" id="COG1850">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031450_2_0_5"/>
    <dbReference type="OMA" id="EYRETYW"/>
    <dbReference type="OrthoDB" id="848380at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016984">
        <property type="term" value="F:ribulose-bisphosphate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019253">
        <property type="term" value="P:reductive pentose-phosphate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01338">
        <property type="entry name" value="RuBisCO_L_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033966">
        <property type="entry name" value="RuBisCO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020878">
        <property type="entry name" value="RuBisCo_large_chain_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000685">
        <property type="entry name" value="RuBisCO_lsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036376">
        <property type="entry name" value="RuBisCO_lsu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017443">
        <property type="entry name" value="RuBisCO_lsu_fd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036422">
        <property type="entry name" value="RuBisCO_lsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020888">
        <property type="entry name" value="RuBisCO_lsuI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42704">
        <property type="entry name" value="PTHR42704"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00016">
        <property type="entry name" value="RuBisCO_large"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02788">
        <property type="entry name" value="RuBisCO_large_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00014">
        <property type="entry name" value="RuBisCO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51649">
        <property type="entry name" value="SSF51649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54966">
        <property type="entry name" value="SSF54966"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00157">
        <property type="entry name" value="RUBISCO_LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0113">Calvin cycle</keyword>
    <keyword id="KW-0120">Carbon dioxide fixation</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0503">Monooxygenase</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000251451" description="Ribulose bisphosphate carboxylase large chain 1">
        <location>
            <begin position="1"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="287"/>
        </location>
    </feature>
    <feature type="binding site" description="in homodimeric partner" evidence="1">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="1">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="372"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="327"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="1">
        <location>
            <position position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01338"/>
        </source>
    </evidence>
    <sequence length="473" mass="52677" checksum="DF83FC650BCEFA82" modified="2005-10-11" version="1">MAVKSYQAGVTQYRQSYWQPDYMPLDTDILACFKITPQPGVDREEAAAAVAAESSCGTWTTVWTDLLTDLDYYKGRAYRLEDVPGDDTCYYAFIAYPIDLFEEGSVVNVFTSLVGNVFGFKAVRALRLEDLRFPIAYVKTCGGPPHGIQVERDKLNKYGRPLLGCTIKPKLGLSAKNYGRACYEALRGGLDFTKDDENINSQPFMRWRDRFDFVMEAVQKAEQETGERKGHYLNVTAPTPEEMYKRAEYAKEIRAPIIMHDYLAGGLCANAGLANWCRNNGMLLHIHRAMHAVIDRNPHHGIHFRVLTKILRLSGGDHLHTGTVVGKLEGDRASTLGWIDLLRESFVPEDRSRGIFFDQDWGSMPGGFAVASGGIHVWHMPALVTIFGDDSVLQFGGGTLGHPWGNAAGAHANRVALEACVQARGEGRHLEKEGKDILTAAAAHSPELKIAMETWKEIKFEFETMDKLAIANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRB9</accession>
    <name>Q3SRB9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Divalent metal cation transporter MntH</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">mntH</name>
        <name evidence="6" type="ordered locus">Nwi_1912</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">H(+)-stimulated, divalent metal cation uptake system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell inner membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NRAMP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1912"/>
    <dbReference type="EnsemblBacteria" id="ABA05172">
        <property type="protein sequence ID" value="ABA05172"/>
        <property type="gene ID" value="Nwi_1912"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1912"/>
    <dbReference type="eggNOG" id="COG1914">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020088_2_0_5"/>
    <dbReference type="OMA" id="IATFVNS"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046873">
        <property type="term" value="F:metal ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00221">
        <property type="entry name" value="NRAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001046">
        <property type="entry name" value="NRAMP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706">
        <property type="entry name" value="PTHR11706"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01566">
        <property type="entry name" value="Nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00447">
        <property type="entry name" value="NATRESASSCMP"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01197">
        <property type="entry name" value="nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0769">Symport</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="84"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="131"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="188"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="234"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="274"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="324"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="372"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="395"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="429"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="460" mass="48636" checksum="B730E5AEB92D548E" modified="2005-10-11" version="1">MKTPALTPEPVPEPSQGPLSTKAGWHSEASARPSLEEVHATVLVPADGHWLRRLFAFAGPGYMVSVGYMDPGNWATDIAGGSQFGYTLLSVILLSNLMAILLQALAARLGIAVGLDLAQACRASYSRPVNFMLWLACEAAIIACDLAEVIGTAIALQLLFGIPLIGGALIAALDAFLLLLLMNRGFRFLEAFVVALLIVIAVCFAIQIVAAAPPVAEILRGFAPSREIVTNPEMLYIAIGIIGATVMPHNLYLHSSIVQTRAYPRTNEGRRTAIRWATADSTIALMLALFVNAAILIVAAAVFHGSGHTEVAEIGDAFKLLSPLLGLGIASTLFAVALLASGINSTVTATLAGQIVMEGFLHLRMPMWARRLVTRGIAIVPVIVVTAIYGDSGTAQLLVLSQVVLSMQLPFAVIPLVRFVSDRRKMGQFAISTGTAATAWIVAGLIVILNVKLLADTFAG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRH2</accession>
    <name>RS2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsB</name>
        <name type="ordered locus">Nwi_1859</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315115.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRH2"/>
    <dbReference type="SMR" id="Q3SRH2"/>
    <dbReference type="STRING" id="323098.Nwi_1859"/>
    <dbReference type="EnsemblBacteria" id="ABA05119">
        <property type="protein sequence ID" value="ABA05119"/>
        <property type="gene ID" value="Nwi_1859"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1859"/>
    <dbReference type="eggNOG" id="COG0052">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040318_2_1_5"/>
    <dbReference type="OMA" id="SYIDRAY"/>
    <dbReference type="OrthoDB" id="1623045at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01425">
        <property type="entry name" value="RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00291_B">
        <property type="entry name" value="Ribosomal_S2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_S2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005706">
        <property type="entry name" value="Ribosomal_S2_bac/mit/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018130">
        <property type="entry name" value="Ribosomal_S2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_S2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12534">
        <property type="entry name" value="PTHR12534"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="SSF52313"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01011">
        <property type="entry name" value="rpsB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00962">
        <property type="entry name" value="RIBOSOMAL_S2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00963">
        <property type="entry name" value="RIBOSOMAL_S2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_1000004010" description="30S ribosomal protein S2">
        <location>
            <begin position="1"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00291"/>
        </source>
    </evidence>
    <sequence length="332" mass="36090" checksum="70D47261C2FD7455" modified="2005-10-11" version="1">MAIPEFTMRQLLEAGVHFGHQSHRWNPKMAEYIFGARNNIHIIDLAQTVPLLHHALQAVSDTVARGGRILFVGTKRQAQDGVADAAKRSAQYFVNSRWLGGTLTNWKTISGSIKRLRHLDEVLNSGDANAYTKKERLTLQRERDKLDRSLGGIKDMGGLPDLMFVIDTNKEDIAIQEAQRLNIPVAAIVDTNCDPKGITYIVPGNDDAGRAISLYCDLIARAAIDGISRAQGESGIDVGASVQPVQEEIPAAAQPAGFQGLAGPRGVADDLKKLTGVSGAIEKKFNDLGIFHYWQLAELDHDTAHRIGEEVGLPSRADGWVAQAKAMTAEAE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-06-10" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRH5</accession>
    <name>RRF_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosome-recycling factor</fullName>
            <shortName evidence="1">RRF</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Ribosome-releasing factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">frr</name>
        <name type="ordered locus">Nwi_1856</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315112.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRH5"/>
    <dbReference type="SMR" id="Q3SRH5"/>
    <dbReference type="STRING" id="323098.Nwi_1856"/>
    <dbReference type="EnsemblBacteria" id="ABA05116">
        <property type="protein sequence ID" value="ABA05116"/>
        <property type="gene ID" value="Nwi_1856"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1856"/>
    <dbReference type="eggNOG" id="COG0233">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073981_2_0_5"/>
    <dbReference type="OMA" id="FNPMNNG"/>
    <dbReference type="OrthoDB" id="1674344at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006415">
        <property type="term" value="P:translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00520">
        <property type="entry name" value="RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00040">
        <property type="entry name" value="RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002661">
        <property type="entry name" value="Ribosome_recyc_fac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023584">
        <property type="entry name" value="Ribosome_recyc_fac_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036191">
        <property type="entry name" value="RRF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20982">
        <property type="entry name" value="PTHR20982"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01765">
        <property type="entry name" value="RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55194">
        <property type="entry name" value="SSF55194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00496">
        <property type="entry name" value="frr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000341024" description="Ribosome-recycling factor">
        <location>
            <begin position="1"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00040"/>
        </source>
    </evidence>
    <sequence length="187" mass="20714" checksum="316DF707394BAB4A" modified="2005-10-11" version="1">MVSGSFDIGDLKRRMQGATQSLKHELGGLRTGRASSSMLDPVQVDAYGSHMPLNQVATISVPEPRLLSVQVWDKSMVKAVEKAIVDSNLGLSPATEGQVLRLRIPELNEERRKELVKVAHKYAEAAKVAVRHVRRDGLDTLKKLEKNHEISEDDEKRLANDVQKATDSVISEIDQLLAGKEKEILTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRK9</accession>
    <name>Q3SRK9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Isoprenylcysteine carboxyl methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_1821</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315078.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1821"/>
    <dbReference type="EnsemblBacteria" id="ABA05082">
        <property type="protein sequence ID" value="ABA05082"/>
        <property type="gene ID" value="Nwi_1821"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1821"/>
    <dbReference type="eggNOG" id="COG2020">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065200_1_1_5"/>
    <dbReference type="OMA" id="FSLWLFR"/>
    <dbReference type="OrthoDB" id="1734625at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004671">
        <property type="term" value="F:protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007269">
        <property type="entry name" value="ICMT_MeTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04140">
        <property type="entry name" value="ICMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="133"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="195" mass="22339" checksum="851BE786611A5692" modified="2005-10-11" version="1">MSSITLGQTIWLIGLVAWFIIRYPYARRARGTATLRRDGTLRERLLLAAAFAGLFVIPMVNLATGWPRAFAYDPGYGLVVLGSLLFAFSLWLFRRSHKDLGRQWSASLEVREGHRVVRSGVYRSIRHPMYASFWLWALAQACLLPNVVAAFSGVAAVALLFFARVRFEEQMMIETFGEDYRRYMQQTHRIIPGVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRL9</accession>
    <name>Q3SRL9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Sel1-like repeat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1811</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1811"/>
    <dbReference type="EnsemblBacteria" id="ABA05072">
        <property type="protein sequence ID" value="ABA05072"/>
        <property type="gene ID" value="Nwi_1811"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1811"/>
    <dbReference type="eggNOG" id="COG0790">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067747_0_0_5"/>
    <dbReference type="OMA" id="GTYFLEG"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="256" mass="27623" checksum="AC37AE1E5D13B8B1" modified="2005-10-11" version="1">MCVSAPAFAFDGTVTGEQEAAAALPVVSAPLAGGEALKKAATPSVSLTALQYAAEGGHPIAQWRLGRMYAKGDGVTQDDLRAFQYFSRIANAHAEDSPSAPQAAVVANAFVALGRYYLQGIPNSAIKRDAERAREMFSYAASYFGNADAQYDLARLYLKGIGAPHDVKYGARWLGLAAQKGQHQAQAMLGRMLFNGDLLPRQAARGLMWLTLARDAAAPDETWITESYNKAIAKASEDDRAMALQMLEHWVQGRRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRN3</accession>
    <name>Q3SRN3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="3">PPIase</shortName>
            <ecNumber evidence="3">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_1797</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 3">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315054.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1797"/>
    <dbReference type="EnsemblBacteria" id="ABA05058">
        <property type="protein sequence ID" value="ABA05058"/>
        <property type="gene ID" value="Nwi_1797"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1797"/>
    <dbReference type="eggNOG" id="COG0652">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_16_6_5"/>
    <dbReference type="OMA" id="SIVSMRV"/>
    <dbReference type="OrthoDB" id="1861282at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000413">
        <property type="term" value="P:protein peptidyl-prolyl isomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PTHR45625"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="SSF50891"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="4">
        <location>
            <begin position="15"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="154" mass="16758" checksum="05B0D99253AF545A" modified="2005-10-11" version="1">MADSENTLILETTQGPVTIEMRPDLAPGHVARIKELVREGFYDGIVFHRVIDGFMAQTGCPQGTGMGGSGQKLKAEFNREPHVRGTASMARAQSPDSGDSQFFICFDNASFLDGQYTVWGKVIEGMENVDKIKRGEPVQNPDKIVKARIAADAA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-09-11" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRQ2</accession>
    <name>TATB_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sec-independent protein translocase protein TatB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tatB</name>
        <name type="ordered locus">Nwi_1778</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes. Together with TatC, TatB is part of a receptor directly interacting with Tat signal peptides. TatB may form an oligomeric binding site that transiently accommodates folded Tat precursor proteins before their translocation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The Tat system comprises two distinct complexes: a TatABC complex, containing multiple copies of TatA, TatB and TatC subunits, and a separate TatA complex, containing only TatA subunits. Substrates initially bind to the TatABC complex, which probably triggers association of the separate TatA complex to form the active translocon.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TatB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315035.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRQ2"/>
    <dbReference type="SMR" id="Q3SRQ2"/>
    <dbReference type="STRING" id="323098.Nwi_1778"/>
    <dbReference type="EnsemblBacteria" id="ABA05039">
        <property type="protein sequence ID" value="ABA05039"/>
        <property type="gene ID" value="Nwi_1778"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1778"/>
    <dbReference type="eggNOG" id="COG1826">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086034_1_3_5"/>
    <dbReference type="OMA" id="PLESMIA"/>
    <dbReference type="OrthoDB" id="2073616at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033281">
        <property type="term" value="C:TAT protein transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043953">
        <property type="term" value="P:protein transport by the Tat complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00237">
        <property type="entry name" value="TatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003369">
        <property type="entry name" value="TatA/B/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018448">
        <property type="entry name" value="TatB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02416">
        <property type="entry name" value="TatA_B_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01410">
        <property type="entry name" value="tatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0653">Protein transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0811">Translocation</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000301198" description="Sec-independent protein translocase protein TatB">
        <location>
            <begin position="1"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="77"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="133"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="163"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="180" mass="18709" checksum="22D494B4B68E106B" modified="2005-10-11" version="1">MFDIGWSELLVIGVVALIAIGPKELPGVLRTVGQWMGKARRMAAEFQGQFNEAMREAEMADLKKSFDEVRDATSGLTRGDLMTRLTDSLGEPPRLEDIDKPATGADKPSVSSDAASASGSAAPEAGAAETFTADAGTNASGPLAITPQAHQDATAVEPPEGAPEISREGRDQTARGAKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRS7</accession>
    <name>Q3SRS7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Biotin carboxylase</fullName>
            <ecNumber evidence="9">6.3.4.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 9">Acetyl-coenzyme A carboxylase biotin carboxylase subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_1753</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + hydrogencarbonate + N(6)-biotinyl-L-lysyl-[protein] = ADP + H(+) + N(6)-carboxybiotinyl-L-lysyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13501"/>
            <dbReference type="Rhea" id="RHEA-COMP:10505"/>
            <dbReference type="Rhea" id="RHEA-COMP:10506"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83144"/>
            <dbReference type="ChEBI" id="CHEBI:83145"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.14"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 9">Acetyl-CoA carboxylase is a heterohexamer of biotin carboxyl carrier protein, biotin carboxylase and the two subunits of carboxyl transferase in a 2:2 complex.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.14" evidence="9"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315010.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1753"/>
    <dbReference type="EnsemblBacteria" id="ABA05014">
        <property type="protein sequence ID" value="ABA05014"/>
        <property type="gene ID" value="Nwi_1753"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1753"/>
    <dbReference type="eggNOG" id="COG0439">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000395_3_2_5"/>
    <dbReference type="OMA" id="ITHFHTP"/>
    <dbReference type="OrthoDB" id="361205at2"/>
    <dbReference type="UniPathway" id="UPA00655">
        <property type="reaction ID" value="UER00711"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004075">
        <property type="term" value="F:biotin carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001295">
        <property type="term" value="P:malonyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004549">
        <property type="entry name" value="Acetyl_CoA_COase_biotin_COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CbamoylP_synth_lsu-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="SSF51246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00514">
        <property type="entry name" value="accC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0092">Biotin</keyword>
    <keyword evidence="9" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4 9 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="11">
        <location>
            <begin position="1"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="10">
        <location>
            <begin position="120"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA05014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="451" mass="48774" checksum="A485290E250953C2" modified="2005-10-11" version="1">MFDKILIANRGEIALRILRACKELGIATVAVHSTADADAMHVRLADESVCIGPPTTKDSYLNIPALLAACEITGAEAVHPGYGFLSENARFAEILADHRLHFIGPKAEHIRLMGDKIEAKKTAKRLGIPVVPGSDGGIGPDDNAMAIAKAIGFPVLVKAAAGGGGRGMKIVKTPDDLMMAISAAGNEARAAFGDASVYLEKYLQKPRHIEIQVLGDGRGGAIHLGERDCSLQRRHQKVWEEGPSPALTAAARNKIGETVAKAMSEIKYLGVGTVEFLYEDGRFYFIEMNTRIQVEHPVTEMITDIDLVLEQIRIAAGGDLPAAQKEIAVNGHAIECRINAENPVTFRPSPGKILQFHPPGGLGVRIDSAVYQGYVIPPYYDSLVGKLIVHGKTRAECLMRLRRALDEMVVEGVETTLPLFRALVREASIIDGDYHIHWLEQYLSSGGLKGA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRY9</accession>
    <name>RS6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsF</name>
        <name type="ordered locus">Nwi_1691</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds together with S18 to 16S ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314950.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRY9"/>
    <dbReference type="SMR" id="Q3SRY9"/>
    <dbReference type="STRING" id="323098.Nwi_1691"/>
    <dbReference type="EnsemblBacteria" id="ABA04952">
        <property type="protein sequence ID" value="ABA04952"/>
        <property type="gene ID" value="Nwi_1691"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1691"/>
    <dbReference type="eggNOG" id="COG0360">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113441_2_0_5"/>
    <dbReference type="OMA" id="ITEASPM"/>
    <dbReference type="OrthoDB" id="1776954at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00473">
        <property type="entry name" value="bS6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00360">
        <property type="entry name" value="Ribosomal_S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000529">
        <property type="entry name" value="Ribosomal_S6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020814">
        <property type="entry name" value="Ribosomal_S6_plastid/chlpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035980">
        <property type="entry name" value="Ribosomal_S6_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribosomal_S6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21011">
        <property type="entry name" value="PTHR21011"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01250">
        <property type="entry name" value="Ribosomal_S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54995">
        <property type="entry name" value="SSF54995"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00166">
        <property type="entry name" value="S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000229556" description="30S ribosomal protein S6">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="97"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="139" mass="15955" checksum="7E955BBA77F65A25" modified="2005-10-11" version="1">MPLYEHVFLARQDASSQQVEELTTQITGVITGLGGKVTKTENWGVRSLTYRMKKNRKAHFVLLNIDGPATIVNEIERQERIHEDVIRYLTVRVDEHEDGPSAMMRKADRDRERDDRGGGFRGDRGPRRPREEPAAVVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS01</accession>
    <name>Q3SS01_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">LPS-assembly protein LptD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lptD</name>
        <name evidence="5" type="ordered locus">Nwi_1679</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the assembly of lipopolysaccharide (LPS) at the surface of the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the lipopolysaccharide transport and assembly complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LptD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314939.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1679"/>
    <dbReference type="EnsemblBacteria" id="ABA04940">
        <property type="protein sequence ID" value="ABA04940"/>
        <property type="gene ID" value="Nwi_1679"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1679"/>
    <dbReference type="eggNOG" id="COG1452">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009039_3_0_5"/>
    <dbReference type="OMA" id="DYSHLDW"/>
    <dbReference type="OrthoDB" id="100018at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043165">
        <property type="term" value="P:Gram-negative-bacterium-type cell outer membrane assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015920">
        <property type="term" value="P:lipopolysaccharide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010033">
        <property type="term" value="P:response to organic substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01411">
        <property type="entry name" value="LPS_assembly_LptD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020889">
        <property type="entry name" value="LipoPS_assembly_LptD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007543">
        <property type="entry name" value="LptD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005653">
        <property type="entry name" value="OstA-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04453">
        <property type="entry name" value="LptD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03968">
        <property type="entry name" value="LptD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="OstA-like_N" evidence="3">
        <location>
            <begin position="82"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="LptD" evidence="4">
        <location>
            <begin position="335"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="829" mass="91869" checksum="FA276E7C20BF3BEE" modified="2005-10-11" version="1">MAPIATLRKRSAIRERRSPLRCYRSGASVIPASLTAAVLGLIAAAVMDVVATTPAAAQSFTYNPRPPKPAPPPRQNDGQMLVQATELDYDYNNSRVSAVGNVQMFYNGTSVEADRVIYDQKTKRLHAEGNIRMTDADGKVTYANILDLSDDYRDGFVDSLRVDTADATRMAATRADRTEGNYTVFENGVYTACAPCKENPKKPPLWQVKGARIIHNQTEKMLYFEDARLEFYGVPMAYMPFFSTPDPTVKRKSGFLMPAVTAFTGFGVGVETPYYFALAPNYDLTISPRFTTRQGVLMHGEFRQRFEDGAYQIRAYGINQLDPDAFGAGPGNRDFRGGVDTKGQFALNDKWIWGWDGVLLSDYFFFSDYRLRQYRDPMQSFLTLPTEALSQLYLTGVGNRSYFDARAVHYLSFSGNQSLVPVIAPVVDYSNVINHSVIGGEFSYKFNFVNLMRDQPQFDPISTLANTSFVCGPTSADPAARPRDLCLLRGIPGTYTRLTAQAQWRRSFTDSFGQIWTPFASLRADAINADISNQPGVSEFLQPGATQTVRLMPAVGLEYRYPFINVQPWGTTTLEPIAQVIIRPNESYAGRLPNEDAQSLVFDATNLFRVDKFSGYDRVEGGGRANVGVQATTQFDRGGAINFLFGQSYHLFGLNSFAVADMTNTGVNSGLQRAQSDYVTSVNYSPNSAFTFSTRARIDETTLNVQRFEAEGRASFNRWSGSILYGDYAEQPNLGFLTRRRGILGSGSIKIASNWVVQGAARWDLEARQINQYAVGAGYVDDCFVLGVNYVTSYIYAAGAAPPQLNHAFMLQLGLRTLGQTRQGSTGVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS28</accession>
    <name>Q3SS28_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DedA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1652</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the DedA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314912.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1652"/>
    <dbReference type="EnsemblBacteria" id="ABA04913">
        <property type="protein sequence ID" value="ABA04913"/>
        <property type="gene ID" value="Nwi_1652"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1652"/>
    <dbReference type="eggNOG" id="COG0586">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044208_3_1_5"/>
    <dbReference type="OMA" id="WGMWAVF"/>
    <dbReference type="OrthoDB" id="1928533at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032818">
        <property type="entry name" value="DedA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="SNARE_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30353">
        <property type="entry name" value="PTHR30353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="SNARE_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="20"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="176" mass="19553" checksum="5DD0E71E7C6DBF55" modified="2005-10-11" version="1">MEDVARSLVDFVRDHQVWAAPVVLLLAFGESIAFVSLLIPAWGILVAIGAMMGPGQINFWPVWLAGAIGAALGDWVSYWIGFTFKDQVASIWPLSRHPGLLPRGIAFVEKWGVPSIYIGRFFGPLRATVPLAAGIFEMPSLRFQFANWTSALIWSAVLLFFGDLTSQFVQWLWRAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS49</accession>
    <name>Q3SS49_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1631</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1631"/>
    <dbReference type="EnsemblBacteria" id="ABA04892">
        <property type="protein sequence ID" value="ABA04892"/>
        <property type="gene ID" value="Nwi_1631"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1631"/>
    <dbReference type="HOGENOM" id="CLU_2465913_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="88" mass="9729" checksum="DEE16D637C7C880A" modified="2005-10-11" version="1">MTRNPFQELGDRVRAQAAATCEALGVDNLNEVRFSKPPGIEETFAKVKSATPDHVREGIIDAVYLIVAAETHDPRVLAIRRKLFGDPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS63</accession>
    <name>Q3SS63_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phage terminase, small subunit, putative, P27</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1617</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314882.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1617"/>
    <dbReference type="EnsemblBacteria" id="ABA04878">
        <property type="protein sequence ID" value="ABA04878"/>
        <property type="gene ID" value="Nwi_1617"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1617"/>
    <dbReference type="eggNOG" id="COG3747">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107958_4_1_5"/>
    <dbReference type="OMA" id="AYTYQAN"/>
    <dbReference type="OrthoDB" id="1570231at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006448">
        <property type="entry name" value="Phage_term_ssu_P27"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05119">
        <property type="entry name" value="Terminase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01558">
        <property type="entry name" value="sm_term_P27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="131" mass="14614" checksum="78702AE9C331CEB4" modified="2005-10-11" version="1">MKGAKPHLRTDAAAMKRAPTVPKWLSADAKREWKRVLPDLIERRILTDADLGSLENYCIAIGQVREMERILQTEGHVYGAESGLLKRHPAGVILSDAMTRARLLAVEMGLTPVSRSRPAIRDDDEEDSLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS69</accession>
    <name>Q3SS69_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glyoxalase/bleomycin resistance protein/dioxygenase</fullName>
            <ecNumber evidence="2">4.4.1.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1611</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.4.1.5" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314876.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1611"/>
    <dbReference type="EnsemblBacteria" id="ABA04872">
        <property type="protein sequence ID" value="ABA04872"/>
        <property type="gene ID" value="Nwi_1611"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1611"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046006_8_0_5"/>
    <dbReference type="OMA" id="PRDGNMA"/>
    <dbReference type="OrthoDB" id="1984437at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004462">
        <property type="term" value="F:lactoylglutathione lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018146">
        <property type="entry name" value="Glyoxalase_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00934">
        <property type="entry name" value="GLYOXALASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="2"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="149" mass="17241" checksum="88EE8F9D809790D4" modified="2005-10-11" version="1">MRYLHTMLRVRNLEIALKFYVDALGLKEVRRVDNDKGKFTLVFLCAPEDEDLVRQSSGRGAPLVELTYNWVEETYGEDRFFGHLAYEVDDIYATCERLMRLGITINRPPRDGQMAFIRSPDLHSIELLQKGKPLPAAEPWTSMPNTGHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSA3</accession>
    <name>Q3SSA3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NAD-dependent epimerase/dehydratase</fullName>
            <ecNumber evidence="2">1.1.1.100</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1577</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.100" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314844.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1577"/>
    <dbReference type="EnsemblBacteria" id="ABA04838">
        <property type="protein sequence ID" value="ABA04838"/>
        <property type="gene ID" value="Nwi_1577"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1577"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_0_5"/>
    <dbReference type="OMA" id="NIAGTWR"/>
    <dbReference type="OrthoDB" id="1356861at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="226" mass="22815" checksum="58E8DC7A2F3BD77C" modified="2005-10-11" version="1">MQGKVIAVTGASGALGKVVVDAAITRGARAACINRAVARGDATADRIELGGVDLTDPAQALQAIDAAAAHFGRLDVLVNVAGAFAFETVADGDPKTWQRLYAINVATALNASRAAIPHLVASGAGRIVNIGAIGALQAGVGMGAYAASKAAVHRLTEALASELKGKVTVNAVLPSTIDTPANRRDMPKADFATWVTADELADVILFLASDAASGVTGALLPVRGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSF0</accession>
    <name>Q3SSF0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cystathionine beta-lyase</fullName>
            <ecNumber evidence="6">4.4.1.8</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_1530</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L,L-cystathionine = L-homocysteine + NH4(+) + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:13965"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:58161"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.8" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314797.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1530"/>
    <dbReference type="EnsemblBacteria" id="ABA04791">
        <property type="protein sequence ID" value="ABA04791"/>
        <property type="gene ID" value="Nwi_1530"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1530"/>
    <dbReference type="eggNOG" id="COG0626">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018986_5_1_5"/>
    <dbReference type="OMA" id="GPYTYGR"/>
    <dbReference type="OrthoDB" id="637281at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004121">
        <property type="term" value="F:cystathionine beta-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006233">
        <property type="entry name" value="Cys_b_lyase_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43500">
        <property type="entry name" value="PTHR43500"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01324">
        <property type="entry name" value="cysta_beta_ly_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="4">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA04791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="398" mass="42886" checksum="2E3DC02F5C112E9B" modified="2005-10-11" version="1">MTLSKSDGSTDSLRAETRLVVSGRDTASQKGFVNPPIVRGSTVLYPTAGDLYAHRGEFQYGRHGTPSTRALQDALMALEGPHCAGVGLVPSGVSALSTALLSVLKAGDHLLVCDNVYRPTRQFCDGFLARYGVETTYFDPLIGSGIAALLRPETRVVMVEAPGSQSLEMPDVPAIAAAAHARGAIVIDDNTWATPLFRRALEQGVDISIQAATKYIGGHSDVMFGTIAANASAWPALTETIRLLGLCVGPDDVFLTLRGLRTLAVRLYHHQQAAMNMAQWLARRPEVTRILYPAWPDDPGHAIWKRDFTGAPGLFSIVLKPAPQQAVSALLDTLKLFGMGYSWGGFESLIIPFDCASYRTATKWTPGGPTLRLQIGLENVDDLKADLERGFAAFNAHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="126" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSG3</accession>
    <name>Q3SSG3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Glutamine--fructose-6-phosphate aminotransferase [isomerizing]</fullName>
            <ecNumber evidence="2 8">2.6.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">D-fructose-6-phosphate amidotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">GFAT</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Glucosamine-6-phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Hexosephosphate aminotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">L-glutamine--D-fructose-6-phosphate amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">glmS</name>
        <name evidence="11" type="ordered locus">Nwi_1517</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:13237"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="2.6.1.16"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.6.1.16" evidence="2 8"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314784.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1517"/>
    <dbReference type="EnsemblBacteria" id="ABA04778">
        <property type="protein sequence ID" value="ABA04778"/>
        <property type="gene ID" value="Nwi_1517"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1517"/>
    <dbReference type="eggNOG" id="COG0449">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012520_5_2_5"/>
    <dbReference type="OMA" id="ASEYRYA"/>
    <dbReference type="OrthoDB" id="43416at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004360">
        <property type="term" value="F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901137">
        <property type="term" value="P:carbohydrate derivative biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00164">
        <property type="entry name" value="GlmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035490">
        <property type="entry name" value="GlmS/FrlB_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005855">
        <property type="entry name" value="GlmS_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937:SF0">
        <property type="entry name" value="PTHR10937:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01135">
        <property type="entry name" value="glmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 11" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 8 11" id="KW-0808">Transferase</keyword>
    <feature type="initiator methionine" description="Removed" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="9">
        <location>
            <begin position="2"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="10">
        <location>
            <begin position="284"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="10">
        <location>
            <begin position="456"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; for GATase activity" evidence="8">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="active site" description="For Fru-6P isomerization activity" evidence="8">
        <location>
            <position position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA04778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="608" mass="65891" checksum="EA7C851495AA760A" modified="2005-10-11" version="1">MCGIVGILGRGPVAEQLVDSLRRLEYRGYDSAGIATLEGACLARRRAEGKLRNLAGRLQAEPLIGQVGIGHTRWATHGRPTENNAHPHATERVAVVHNGIIENFRELREKLKKNGAVFETETDTEAVLHLVDGYLAAGIDPVEAVRATLSQLRGAFALGFIFAADDDILICARNGPPLAIGYGDGEMYLGSDAIALGPLTDTISYLEDGDWAVLTHDGATVYDHHNAVVQREVVKQSASASLVDKANYRHFMAKEIHEQPEVVGHTLARYVDMATERVSLPVDLPFDFREIKRVSIIACGTANYAGYIGKYWLERLGRLAVEIDIASEFRYREAPLGKGDLAIFISQSGETADTLAALRYAKQHGLHTLSIVNVTTSTIARESETMLQTLAGPEIGVASTKAFTCQLMVLATLAIAAGKVRGELSEEDEARLVHDLIEVPRLMSEALATEPQIDRLAREIAKSRDVLYLGRGTSYPLALEGALKLKEISYIHAEGYAAGELKHGPIALIDENMPVVVIAPYDRVFEKTVSNMQEVAARGGNIILMTDAKGAAEAAVEPLETIIMPDMAATFAALIYAVPVQLLAYHTAVVMGTDVDQPRNLAKSVTVE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSP2</accession>
    <name>3MGH_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative 3-methyladenine DNA glycosylase</fullName>
            <ecNumber evidence="1">3.2.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">Nwi_1438</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA glycosylase MPG family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314707.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSP2"/>
    <dbReference type="SMR" id="Q3SSP2"/>
    <dbReference type="STRING" id="323098.Nwi_1438"/>
    <dbReference type="EnsemblBacteria" id="ABA04699">
        <property type="protein sequence ID" value="ABA04699"/>
        <property type="gene ID" value="Nwi_1438"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1438"/>
    <dbReference type="eggNOG" id="COG2094">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060471_4_1_5"/>
    <dbReference type="OMA" id="VEAYHHT"/>
    <dbReference type="OrthoDB" id="1385409at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003905">
        <property type="term" value="F:alkylbase DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00540">
        <property type="entry name" value="AAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00527">
        <property type="entry name" value="3MGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011034">
        <property type="entry name" value="Formyl_transferase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003180">
        <property type="entry name" value="MPG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036995">
        <property type="entry name" value="MPG_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10429">
        <property type="entry name" value="PTHR10429"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02245">
        <property type="entry name" value="Pur_DNA_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50486">
        <property type="entry name" value="SSF50486"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00567">
        <property type="entry name" value="3mg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000265039" description="Putative 3-methyladenine DNA glycosylase">
        <location>
            <begin position="1"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00527"/>
        </source>
    </evidence>
    <sequence length="208" mass="22512" checksum="C2C405CD4F74801C" modified="2005-10-11" version="1">MKLTQDLTSDGPDPPVLGKPLTRAFFGRSVHDVAPDLIGATLLVDGVGGIIVEVEAYHHTEPAAHSHRGPTPRNMVMFGPPGFAYVYRSYGIHWCVNFVCEMDGSAAAVLIRALQPTHGIPAMRRRRGLHEERLLCSGPGRLCQALGISIAHNALPLDAPPIAVFRRTEKADVVAGVRIGITKAADLPWRYGLKGSKFLSKPFRSAGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSQ9</accession>
    <name>Q3SSQ9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rplM</name>
        <name evidence="5" type="ordered locus">Nwi_1421</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1421"/>
    <dbReference type="EnsemblBacteria" id="ABA04682">
        <property type="protein sequence ID" value="ABA04682"/>
        <property type="gene ID" value="Nwi_1421"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1421"/>
    <dbReference type="eggNOG" id="COG0102">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082184_2_0_5"/>
    <dbReference type="OMA" id="DFVVIIN"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00392">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01366">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_L13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005823">
        <property type="entry name" value="Ribosomal_L13_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036899">
        <property type="entry name" value="Ribosomal_L13_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="PTHR11545"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002181">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52161">
        <property type="entry name" value="SSF52161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01066">
        <property type="entry name" value="rplM_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="157" mass="17481" checksum="7845FF5E9FE27CC0" modified="2005-10-11" version="1">MSHMKTFSAKPAEVTKKWVLIDASGLVVGRLATLVAMRLRGKHLPTYTPHVDCGDNVIIINAAKVVFTGRKRDDKIYYNHTGYIGGIKQRTAKSILEGRFPERVLEKAVERMIPRGPLGRVQLGNLRVYAGAEHPHEAQQPEVINVGAMNRKNMRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSR6</accession>
    <name>Q3SSR6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Polysaccharide export protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1414</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314684.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1414"/>
    <dbReference type="EnsemblBacteria" id="ABA04675">
        <property type="protein sequence ID" value="ABA04675"/>
        <property type="gene ID" value="Nwi_1414"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1414"/>
    <dbReference type="eggNOG" id="COG1596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038343_5_0_5"/>
    <dbReference type="OMA" id="IDYPLCG"/>
    <dbReference type="OrthoDB" id="687725at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015159">
        <property type="term" value="F:polysaccharide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003715">
        <property type="entry name" value="Poly_export"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019554">
        <property type="entry name" value="Soluble_ligand-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02563">
        <property type="entry name" value="Poly_export"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10531">
        <property type="entry name" value="SLBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="SLBB" evidence="2">
        <location>
            <begin position="161"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="234" mass="25137" checksum="D19E533DD421525E" modified="2005-10-11" version="1">MRNLRAVTLGLLTTLALSGCMQHPAPAVMVRPHNDLDAMAYAGPKAPAPVPRRRGLWHTPAFTTAAAPVVYAAAPPPPVIHDPAYRLDAGDRLRIVVYGQEGLTNTYLVDAGGSITMPLIGAVTARGRTPAELSATIAARLRNGYIRQPYVAVEIEAYRPFFILGEVAAPGQYPFVPNMTVESAVAIAGGFSPRARRDRVIVTHTDDRGTVRLMLPPVAPLSPGDTVVVDERWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSR9</accession>
    <name>Q3SSR9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1411</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314681.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1411"/>
    <dbReference type="EnsemblBacteria" id="ABA04672">
        <property type="protein sequence ID" value="ABA04672"/>
        <property type="gene ID" value="Nwi_1411"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1411"/>
    <dbReference type="eggNOG" id="COG3307">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_643880_0_0_5"/>
    <dbReference type="OMA" id="AFMSGGW"/>
    <dbReference type="OrthoDB" id="1879349at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="195"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="333"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="364"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="388"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="420" mass="46464" checksum="C83E9B77748427AA" modified="2005-10-11" version="1">MAYPAAVDSWLRPSTQAPGIAALQRALLWAVGAGGAIVYIEPSPYEFATLAAIVVFFATGLRMRLAFVPLLLLLFLINFGYTIGAVHLMDKTQIVNWIMTSWYMAVTVIFFALVFSEDTETRLDLMRRGLIVGAVIASVAGVAGYFHLVPGEHDVLTLYGRARGTFKDPNVLSAFLILPGLFVLQSVVTDRFGKAFRSALALGIISLAVLLAFSRAAWGQFVLTSAFVLVLMFLTSPSGKQRSRLVLTAVIAAAAIALLLAVLLSLDSVESLFKERASFDQSYDGGRFGRFGRHILGFQMALEQPLGIGPLQFTRFFPEDTHNSYLNAFMSGGWISGISYPVLIFTTVILGFRYIFVRVPWQRTYLAVFAAFLGTVGESFVIDTDHWRHFWLMLGMMWGMFAATHQYRADLRHAPDQPAR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSS2</accession>
    <name>IHFA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integration host factor subunit alpha</fullName>
            <shortName evidence="1">IHF-alpha</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ihfA</name>
        <name evidence="1" type="synonym">himA</name>
        <name type="ordered locus">Nwi_1408</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein is one of the two subunits of integration host factor, a specific DNA-binding protein that functions in genetic recombination as well as in transcriptional and translational control.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of an alpha and a beta chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial histone-like protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314678.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSS2"/>
    <dbReference type="SMR" id="Q3SSS2"/>
    <dbReference type="STRING" id="323098.Nwi_1408"/>
    <dbReference type="EnsemblBacteria" id="ABA04669">
        <property type="protein sequence ID" value="ABA04669"/>
        <property type="gene ID" value="Nwi_1408"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1408"/>
    <dbReference type="eggNOG" id="COG0776">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105066_1_1_5"/>
    <dbReference type="OMA" id="IYVRGFG"/>
    <dbReference type="OrthoDB" id="1847472at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13835">
        <property type="entry name" value="IHF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00380">
        <property type="entry name" value="IHF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000119">
        <property type="entry name" value="Hist_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020816">
        <property type="entry name" value="Histone-like_DNA-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010992">
        <property type="entry name" value="IHF-like_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005684">
        <property type="entry name" value="IHF_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33175">
        <property type="entry name" value="PTHR33175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00216">
        <property type="entry name" value="Bac_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01727">
        <property type="entry name" value="DNABINDINGHU"/>
    </dbReference>
    <dbReference type="SMART" id="SM00411">
        <property type="entry name" value="BHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47729">
        <property type="entry name" value="SSF47729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00987">
        <property type="entry name" value="himA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00045">
        <property type="entry name" value="HISTONE_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <keyword id="KW-0810">Translation regulation</keyword>
    <feature type="chain" id="PRO_1000060551" description="Integration host factor subunit alpha">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00380"/>
        </source>
    </evidence>
    <sequence length="106" mass="11548" checksum="8E8D8BD5C9119E95" modified="2005-10-11" version="1">MTGTGKTVTRVDLCEAVYKKVGLSRTESSAFVELVLKEITDCLEKGETVKLSSFGSFMVRKKGQRIGRNPKTGTEVPISPRRVMVFKPSAILKQRINSNGAGGKTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST02</accession>
    <name>Q3ST02_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nitrogen-fixing NifU-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1328</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314607.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1328"/>
    <dbReference type="EnsemblBacteria" id="ABA04589">
        <property type="protein sequence ID" value="ABA04589"/>
        <property type="gene ID" value="Nwi_1328"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1328"/>
    <dbReference type="eggNOG" id="COG0822">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079283_3_0_5"/>
    <dbReference type="OMA" id="IWLKMDG"/>
    <dbReference type="OrthoDB" id="1793654at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06664">
        <property type="entry name" value="IscU_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002871">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10093">
        <property type="entry name" value="PTHR10093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01592">
        <property type="entry name" value="NifU_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NifU_N" evidence="1">
        <location>
            <begin position="6"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="151" mass="16167" checksum="D42DC9A63268FACB" modified="2005-10-11" version="1">MLNDVYNTRIIELAGNIPRLGRLPAPDASATAHSKLCGSTVKIDIKMDGSVVTDFAHDVKACALGQASSSIMARHVVGSTASELRELREIVRRMLKENGSPPKGKWAEIALLEPVRDYKARHASTLLTFDAVVDAIGQIEAKSAALTTAQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST20</accession>
    <name>Q3ST20_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransf_33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_1310</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1310"/>
    <dbReference type="EnsemblBacteria" id="ABA04571">
        <property type="protein sequence ID" value="ABA04571"/>
        <property type="gene ID" value="Nwi_1310"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1310"/>
    <dbReference type="eggNOG" id="COG4301">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049766_1_1_5"/>
    <dbReference type="OMA" id="RVEMHLV"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035094">
        <property type="entry name" value="EgtD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019257">
        <property type="entry name" value="MeTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017804">
        <property type="entry name" value="MeTrfase_EgtD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10017">
        <property type="entry name" value="Methyltransf_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018005">
        <property type="entry name" value="UCP018005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03438">
        <property type="entry name" value="egtD_ergothio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_33" evidence="4">
        <location>
            <begin position="27"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="327" mass="36339" checksum="87050D355C7FEB70" modified="2005-10-11" version="1">MMNPDAATFSGTPRSNETGSSESTRTFARDAIAGLTHHPKQLPPKYFYDAAGSELFEQITRLPEYYPTRTELGILRDRSAEIASIIPEHAALVEFGAGAPTKARLLLAACEFAAYVPVDISGDFLNAQARGLRKEFPHLEIHPVTADFTAPFHLPDQVAAMPKVGFFPGSTLGNFEPHEANRFLRGARDILGKGAYMLIGVDLEKNERRMYEAYNDAAGVTARFNLNMLARVNRELGGNFDLAAFAHRAIYNRERHRIEMHLVSKKPQTARLHGHSFAFRAGESIHTESSYKYSIERFNALAKGSGWTPVAAWTDPERMFSVHALVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST28</accession>
    <name>Q3ST28_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyanate hydratase</fullName>
            <shortName evidence="3">Cyanase</shortName>
            <ecNumber evidence="3">4.2.1.104</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Cyanate hydrolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Cyanate lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cynS</name>
        <name evidence="5" type="ordered locus">Nwi_1302</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 3">Catalyzes the reaction of cyanate with bicarbonate to produce ammonia and carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>cyanate + 3 H(+) + hydrogencarbonate = 2 CO2 + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:11120"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29195"/>
            <dbReference type="EC" id="4.2.1.104"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cyanase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.104" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314583.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1302"/>
    <dbReference type="EnsemblBacteria" id="ABA04563">
        <property type="protein sequence ID" value="ABA04563"/>
        <property type="gene ID" value="Nwi_1302"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1302"/>
    <dbReference type="eggNOG" id="COG1513">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103452_1_0_5"/>
    <dbReference type="OMA" id="YELVMIN"/>
    <dbReference type="OrthoDB" id="1506439at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008824">
        <property type="term" value="F:cyanate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009439">
        <property type="term" value="P:cyanate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00559">
        <property type="entry name" value="Cyanase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00535">
        <property type="entry name" value="Cyanate_hydrat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008076">
        <property type="entry name" value="Cyanase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003712">
        <property type="entry name" value="Cyanate_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036581">
        <property type="entry name" value="Cyanate_lyase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34186">
        <property type="entry name" value="PTHR34186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02560">
        <property type="entry name" value="Cyanate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001263">
        <property type="entry name" value="Cyanate_hydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01693">
        <property type="entry name" value="CYANASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01116">
        <property type="entry name" value="Cyanate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55234">
        <property type="entry name" value="SSF55234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00673">
        <property type="entry name" value="cynS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 5" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyanate_lyase" evidence="4">
        <location>
            <begin position="76"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="162" mass="17920" checksum="70EB48A42205EAA9" modified="2005-10-11" version="1">MKRSDLTEKILDIKREKEWSWSYIAGEIGGCSDILIVSALLGHMKLTKSQAAVAGKLFGLSKTEIAMLNEVPVRGAGVPMPPTDPLIYRLYELVMINGPALKVLIEEEFGDGIMSAIDFDLELARVASPKGDRVKIGMCGKFLPFKYTAASEQHRERHLEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STC3</accession>
    <name>Q3STC3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glycogen debranching enzyme GlgX</fullName>
            <ecNumber evidence="5">3.2.1.68</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_1206</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 13 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.68" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1206"/>
    <dbReference type="CAZy" id="CBM48">
        <property type="family name" value="Carbohydrate-Binding Module Family 48"/>
    </dbReference>
    <dbReference type="CAZy" id="GH13">
        <property type="family name" value="Glycoside Hydrolase Family 13"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04468">
        <property type="protein sequence ID" value="ABA04468"/>
        <property type="gene ID" value="Nwi_1206"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1206"/>
    <dbReference type="eggNOG" id="COG1523">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011725_1_1_5"/>
    <dbReference type="OMA" id="SEPWDCG"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004133">
        <property type="term" value="F:glycogen debranching enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019156">
        <property type="term" value="F:isoamylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005980">
        <property type="term" value="P:glycogen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02856">
        <property type="entry name" value="E_set_GDE_Isoamylase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044505">
        <property type="entry name" value="GlgX_Isoamylase_N_E_set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="Glyco_hydro_13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004193">
        <property type="entry name" value="Glyco_hydro_13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011837">
        <property type="entry name" value="Glycogen_debranch_GlgX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02922">
        <property type="entry name" value="CBM_48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="SSF81296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02100">
        <property type="entry name" value="glgX_debranch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aamy" evidence="4">
        <location>
            <begin position="139"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="469"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="692" mass="78551" checksum="93F63C7D70D22412" modified="2005-10-11" version="1">MMRVSEGTHVRLGSTWDGRGTNFALFSAHAEKVELCLFDSKGRREIERIALPEHTENIWHCYLNDVAPGQLYGYRVYGPYDPQHGHRFNPHKLLLDPYAKKISGRLVWSDAHFAYRAGSPREDLSFDRRDNARAMPKATVIDETFNWGRREPRPNATWEETIIYEAHVKGLTKTRMDIAPELRGTYGALAAPAMIDHLRRLGVTTIELLPVHGFVDDRHLVERKLSNYWGYNSLSFFAPEQRYAKGAALDEFRMTVARLHDAGFEIMLDVVYNHTAEGNHLGPTLSFRGIDNASYYWLMPDDPRYYDDFTGTGNSLNLTHPRVLQMVMDSLRYWVEVCHIDGFRFDLASTLARGAHGFDRNSAFFNAIRQDPVLANVKLVAEPWDVGPGGYQVGGFPSQWSEWNDRYRSTMRRYWAGEGNLIGDVSRRMTGSSDLFNHDDRAPRAGVNHVTVHDGFTLADLFSYNEKHNEANGEDNRDGSNDNLSNNCGHEGPTDDPAILSLRRQLRKNQLACLLLAQGIPLLLAGDEVGNSQSGNNNAYCQDNEIGWIDWSDLGDRENDMTDFVGHLTAIRRRFAQIRSRQWVTAKGINGSYSVLWLTPEGNEMTEADWNFPEGRFLSYVLGPVESHEQALYVVLNAAPDPIEFRIPNLEEYKTWSIVLHTTDDDVDGVPRHPNTTMQAPPRSVIAFAGQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STE3</accession>
    <name>Q3STE3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1186</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314477.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1186"/>
    <dbReference type="EnsemblBacteria" id="ABA04448">
        <property type="protein sequence ID" value="ABA04448"/>
        <property type="gene ID" value="Nwi_1186"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1186"/>
    <dbReference type="eggNOG" id="ENOG503136X">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2539267_0_0_5"/>
    <dbReference type="OMA" id="IPYVQAI"/>
    <dbReference type="OrthoDB" id="2044885at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="83" mass="8345" checksum="4A1926B574DE84F5" modified="2005-10-11" version="1">MVTAAEVSAGLTAAEAIVNAIIKVAPAIERGVVSSIPYIQAIAGLIGGKNATAEEIDAVLARINAEVDLFLSPLPPDDGSTTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STE8</accession>
    <name>Q3STE8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Gene transfer agent (GTA) orfg15, like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1181</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314472.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1181"/>
    <dbReference type="EnsemblBacteria" id="ABA04443">
        <property type="protein sequence ID" value="ABA04443"/>
        <property type="gene ID" value="Nwi_1181"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1181"/>
    <dbReference type="eggNOG" id="COG3391">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007148_0_0_5"/>
    <dbReference type="OMA" id="RITCDPA"/>
    <dbReference type="OrthoDB" id="646292at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025195">
        <property type="entry name" value="GTA_TIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032876">
        <property type="entry name" value="J_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13547">
        <property type="entry name" value="GTA_TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13550">
        <property type="entry name" value="Phage-tail_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GTA_TIM" evidence="1">
        <location>
            <begin position="419"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="domain" description="Phage-tail_3" evidence="2">
        <location>
            <begin position="778"/>
            <end position="939"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1285" mass="135425" checksum="A8F45F2764046584" modified="2005-10-11" version="1">MAALVLSVAGGAAGALFGPAGAIAGRLAGALIGNAVDRKLFGPGNRTIVGPRLADLDVMASTEGAPIPRVYGRARLSGQVIWATRLEEVVSSETSSSGGKGGGLFSGPSTRTTTYSYFANFAVGLCEGVIGRVGRIWADGSPLDVSGLTFRVHRGTEDQAPDDLIVAKEGAGRAPAYRGLAYVVFERMPLAKFGNRIPQLSFEIIRPIGQLERMVRAMTLIPGTTEFGYEPSAMTRSTGPGQSEPENRHVANAESDVIAALDDLQGACPNLESVAVVVAWFGSDLRAGSCVVRPGVDSAGKITRGGDWSVAGVTRGSAYVVSQVDGRPAFGGTPSDASVRHLIAELKARGLKVTFYPFVMMDLPAGNGLTDPWSGASSQPAYPWRGRITCDPAPGQPGSPQGTATAAAQVASFFTGGSWNYRRMILHYANLVVSAGGVDAFLIGSELRSLTRVRSGAGVYPAVDALVTLAAEVKSIVGSSTVVTYGADWTEYGADVVDAGASEVRFPLDALWASSAIDAVGIDYYMPLADWRDEAGHLDFHIAASTYDLGYLAGNVYGGEGFDWYYTDGTARAAQTRTPITDGLGKPWTFRVKDIRSWWSSAHHERVGGVEVSSPTAWVPQSKPVWLTEAGCPAVDKGANQPNVFPDPKSSENFLPYFSSGSRDDLIQRRYLQALLGKLDPSFGAADADNPVSSIYGKRMIEPSAIYLWTWDARPYPAFPAATEVWSDGPNWQTGHWLTGRLGAAPMDALVQALLTDSGVTGADTSALRESCDGYVVDRPMSPRAMIEPLAGAYAFDASAADGTLCFVSRGGAPVAEFAEGDLVLTNGGGLAQLTRAQETELPREARFSFTDGTVDYRRSAVTSRRLAGGSNRSVHSDLAVVGDDAAMARRAEIWLQDLWAGRESAAFSLGPRCLSLTPGDVIAVTINGRRRLFEISALLDAEARQVSARSIDPEVFSVPLSVPRPTRPAIPAALGPVQVSVLDLPMIEPSEPPILTRLAVFAKPWPTSVTAWTSFDGAGFQPAAIIPAPSTSGETLDPLPAGPSGRWDRGNAVRVRLYGGALTSVSDARVLAGANAAAVQNPDGDWEVLQFAHAELVDGGVYKLSRLLRGQAGSDHAIADVLPPGAPFVLLNTRLSPLAKGLGALDRPILVRLAAAGRAHDDPTAVALTVTPRATALRPLSPVHVKAVRESGGVRISWIRRTRIDGDGWSGEVPLGEDVEAYTLDVFSGGAIVRSIACGAPEAFYPEADELADFGAPQASLHLRVAQISATVGAGHPTELTLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STI3</accession>
    <name>Q3STI3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1146</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314438.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04408">
        <property type="protein sequence ID" value="ABA04408"/>
        <property type="gene ID" value="Nwi_1146"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1146"/>
    <dbReference type="HOGENOM" id="CLU_2718249_0_0_5"/>
    <dbReference type="OMA" id="WIKRRTK"/>
    <dbReference type="OrthoDB" id="2086179at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="73" mass="8501" checksum="BED72BDD43A4AC1B" modified="2005-10-11" version="1">MTILRWIKRRTKSRYADDKAVDDFERVARDQNAPLDYDQDKRLAELLSSDEDPADNLDEIAKLMGQSEETSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STI7</accession>
    <name>Q3STI7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1142</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314435.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1142"/>
    <dbReference type="EnsemblBacteria" id="ABA04404">
        <property type="protein sequence ID" value="ABA04404"/>
        <property type="gene ID" value="Nwi_1142"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1142"/>
    <dbReference type="HOGENOM" id="CLU_2753795_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="74" mass="8136" checksum="21B4F3689AD57F16" modified="2005-10-11" version="1">MCVAQDIALRTHHLAALRFLAKDPDRIRLIEDENTLAAALCYVDLEKRGFVTIDNEDGMLVTITPAGLARLGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STL5</accession>
    <name>Q3STL5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1114</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314407.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1114"/>
    <dbReference type="EnsemblBacteria" id="ABA04376">
        <property type="protein sequence ID" value="ABA04376"/>
        <property type="gene ID" value="Nwi_1114"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1114"/>
    <dbReference type="eggNOG" id="ENOG5033AXH">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154609_0_0_5"/>
    <dbReference type="OMA" id="MFAVESC"/>
    <dbReference type="OrthoDB" id="1960973at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="111" mass="12124" checksum="9855B4D558A29A8B" modified="2005-10-11" version="1">MTNANTNSTCLEPLFHPAAHYASPDEVLSDDQLSTQEKRIILSSWASDMFAVESCPALRDIPGMGRPIRLADILAALRRLDGGDDDPPPHGGISMRLRRPWAAAPRSAARF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STL6</accession>
    <name>Q3STL6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RNA polymerase-binding transcription factor DksA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">dksA</name>
        <name evidence="8" type="ordered locus">Nwi_1113</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Transcription factor that acts by binding directly to the RNA polymerase (RNAP). Required for negative regulation of rRNA expression and positive regulation of several amino acid biosynthesis promoters.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts directly with the RNA polymerase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DksA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314406.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1113"/>
    <dbReference type="EnsemblBacteria" id="ABA04375">
        <property type="protein sequence ID" value="ABA04375"/>
        <property type="gene ID" value="Nwi_1113"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1113"/>
    <dbReference type="eggNOG" id="COG1734">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043144_2_2_5"/>
    <dbReference type="OMA" id="KKGEEYM"/>
    <dbReference type="OrthoDB" id="1932026at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00926">
        <property type="entry name" value="DksA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012784">
        <property type="entry name" value="DksA_RNA_pol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037187">
        <property type="entry name" value="DnaK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000962">
        <property type="entry name" value="Znf_DskA_TraR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020458">
        <property type="entry name" value="Znf_DskA_TraR_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01258">
        <property type="entry name" value="zf-dskA_traR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109635">
        <property type="entry name" value="SSF109635"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02420">
        <property type="entry name" value="dksA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01102">
        <property type="entry name" value="ZF_DKSA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51128">
        <property type="entry name" value="ZF_DKSA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DksA C4-type" evidence="7">
        <location>
            <begin position="55"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="dksA C4-type" evidence="6">
        <location>
            <begin position="98"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA04375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="136" mass="16238" checksum="67E1ECA898F176CD" modified="2005-10-11" version="1">MEKLKNYRPSEKEPFMNERQREYFRAKLLAWKDEILRESKVTLQTLQEENVNLPDLADRASSETDRAIELRARDRQRKLISKIDAALQRIEDNTYGYCEETGEPISLKRLEARPIATLSVEAQERHEKREKIYRDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STM0</accession>
    <name>Q3STM0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1109</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314402.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1109"/>
    <dbReference type="EnsemblBacteria" id="ABA04371">
        <property type="protein sequence ID" value="ABA04371"/>
        <property type="gene ID" value="Nwi_1109"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1109"/>
    <dbReference type="eggNOG" id="ENOG5032QZ3">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136206_0_0_5"/>
    <dbReference type="OMA" id="ASAYMNG"/>
    <dbReference type="OrthoDB" id="1673021at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="163" mass="18021" checksum="0583D9C118A3418C" modified="2005-10-11" version="1">MTPQSRNPQESPLLPPVSTPADARKLADDMLDIMNALLAVIERETELVRAGKIREGMALESRKTELSRRYVDAIRQFKASQAYMTQSTPDLLTALQRHHDTFRAMLQVNLTVLATAHAVSEAIVRGVSAEAQRRATPQTYTASGQRSAPSRQHMSPIAVSRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STR1</accession>
    <name>Q3STR1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphoheptose isomerase</fullName>
            <ecNumber evidence="9">5.3.1.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Sedoheptulose 7-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">gmhA</name>
        <name evidence="11" type="ordered locus">Nwi_1068</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the isomerization of sedoheptulose 7-phosphate in D-glycero-D-manno-heptose 7-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>2 D-sedoheptulose 7-phosphate = D-glycero-alpha-D-manno-heptose 7-phosphate + D-glycero-beta-D-manno-heptose 7-phosphate</text>
            <dbReference type="Rhea" id="RHEA:27489"/>
            <dbReference type="ChEBI" id="CHEBI:57483"/>
            <dbReference type="ChEBI" id="CHEBI:60203"/>
            <dbReference type="ChEBI" id="CHEBI:60204"/>
            <dbReference type="EC" id="5.3.1.28"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Carbohydrate biosynthesis; D-glycero-D-manno-heptose 7-phosphate biosynthesis; D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate from sedoheptulose 7-phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="9">The reaction produces a racemic mixture of D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the SIS family. GmhA subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.28" evidence="9"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314364.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1068"/>
    <dbReference type="EnsemblBacteria" id="ABA04330">
        <property type="protein sequence ID" value="ABA04330"/>
        <property type="gene ID" value="Nwi_1068"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1068"/>
    <dbReference type="eggNOG" id="COG0279">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080999_4_0_5"/>
    <dbReference type="OMA" id="DVHICVP"/>
    <dbReference type="OrthoDB" id="1571626at2"/>
    <dbReference type="UniPathway" id="UPA00041">
        <property type="reaction ID" value="UER00436"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008968">
        <property type="term" value="F:D-sedoheptulose 7-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001061">
        <property type="term" value="P:D-glycero-D-manno-heptose 7-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05006">
        <property type="entry name" value="SIS_GmhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00067">
        <property type="entry name" value="GmhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035461">
        <property type="entry name" value="GmhA/DiaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004515">
        <property type="entry name" value="Phosphoheptose_Isoase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13580">
        <property type="entry name" value="SIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9 11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SIS" evidence="10">
        <location>
            <begin position="37"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="52"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="94"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="120"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA04330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="197" mass="20743" checksum="D62CE7B3B245414C" modified="2005-10-11" version="1">MSQNPIAVHFQQSLAALQRAANDTTLLAAASEIAVTIIDALRSGNKVLLIGNGGSAADAQHIASEIVGRYRKERPGYAAIALTTDTSALTAISNDYGFERIFCRQIESLGRPGDILLALSTSGRSPNILTALDAARRLGLVTAGFTGLKGQSLREVCDHLLVAPSDDTPVIQQIHMTAAHAICDTVEHALADGDSDR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STS8</accession>
    <name>MURG_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase</fullName>
            <ecNumber evidence="1">2.4.1.227</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Undecaprenyl-PP-MurNAc-pentapeptide-UDPGlcNAc GlcNAc transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">murG</name>
        <name type="ordered locus">Nwi_1051</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc-(pentapeptide)GlcNAc (lipid intermediate II).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>di-trans-octa-cis-undecaprenyl diphospho-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine + UDP-N-acetyl-alpha-D-glucosamine = di-trans-octa-cis-undecaprenyl diphospho-[N-acetyl-alpha-D-glucosaminyl-(1-&gt;4)]-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine + H(+) + UDP</text>
            <dbReference type="Rhea" id="RHEA:31227"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:61387"/>
            <dbReference type="ChEBI" id="CHEBI:61388"/>
            <dbReference type="EC" id="2.4.1.227"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 28 family. MurG subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="ABA04313" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.4.1.227" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04313.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_041345416.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3STS8"/>
    <dbReference type="SMR" id="Q3STS8"/>
    <dbReference type="STRING" id="323098.Nwi_1051"/>
    <dbReference type="CAZy" id="GT28">
        <property type="family name" value="Glycosyltransferase Family 28"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04313">
        <property type="protein sequence ID" value="ABA04313"/>
        <property type="gene ID" value="Nwi_1051"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1051"/>
    <dbReference type="eggNOG" id="COG0707">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037404_2_1_5"/>
    <dbReference type="OrthoDB" id="1165736at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051991">
        <property type="term" value="F:UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol 4-beta-N-acetylglucosaminlytransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050511">
        <property type="term" value="F:undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030259">
        <property type="term" value="P:lipid glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00033">
        <property type="entry name" value="MurG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006009">
        <property type="entry name" value="GlcNAc_MurG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007235">
        <property type="entry name" value="Glyco_trans_28_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004276">
        <property type="entry name" value="GlycoTrans_28_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04101">
        <property type="entry name" value="Glyco_tran_28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03033">
        <property type="entry name" value="Glyco_transf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0133">Cell shape</keyword>
    <keyword id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000225070" description="UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase">
        <location>
            <begin position="1"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="14"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="368" mass="38217" checksum="16EE1008DECC94DE" modified="2006-03-07" version="2">MTTAPLILLAAGGTGGHLFPAEALGVELMKRGLRVRLVTDSRALRYSGLFSKDLTDVVPSETVRGRSPWALARTGLMLAAGTAVALNLMRVLKPAAVVGFGGYPTLPPLIAARLRGIPTVIHDSNAVMGRANSFLSKRVNAIATSLPGVLDKKPSLAGKITTTGTPMRPAILAAAAVPFATPGLGDPLRVLVVGGSQGARVMSDIVPGAIERLEPALRQRLILTQQVRDEDMARVRGFYDRLEIKAELAPFFADLPARLASNHIIISRSGAGTVAELGAIGRPSILVPLPGAIDQDQFANAGVLADVGGAIRIVQPDFTPDRLAAELSALAAGPARLAAMATAARTVGRLDAAGRLADLVMRVARTGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STU7</accession>
    <name>Q3STU7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1032</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314328.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1032"/>
    <dbReference type="EnsemblBacteria" id="ABA04294">
        <property type="protein sequence ID" value="ABA04294"/>
        <property type="gene ID" value="Nwi_1032"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1032"/>
    <dbReference type="eggNOG" id="COG3218">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2288584_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="101" mass="11296" checksum="A3842256AA7E1595" modified="2005-10-11" version="1">MWADSIPNLLQAKLLESFENYDIAHAPLRSMDGVQADHQLLIDVRRFQITTDPEPVADIGLNKDVKVVAARLFEETQKLRTIEPDTASAAFNEAFDGSPRT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STY0</accession>
    <name>RS21_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsU</name>
        <name type="ordered locus">Nwi_0999</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS21 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009799172.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3STY0"/>
    <dbReference type="SMR" id="Q3STY0"/>
    <dbReference type="STRING" id="323098.Nwi_0999"/>
    <dbReference type="EnsemblBacteria" id="ABA04261">
        <property type="protein sequence ID" value="ABA04261"/>
        <property type="gene ID" value="Nwi_0999"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0999"/>
    <dbReference type="eggNOG" id="COG0828">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159258_0_1_5"/>
    <dbReference type="OMA" id="RGYYEKP"/>
    <dbReference type="OrthoDB" id="2088764at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00358">
        <property type="entry name" value="Ribosomal_S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001911">
        <property type="entry name" value="Ribosomal_S21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018278">
        <property type="entry name" value="Ribosomal_S21_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038380">
        <property type="entry name" value="S21_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21109">
        <property type="entry name" value="PTHR21109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01165">
        <property type="entry name" value="Ribosomal_S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00030">
        <property type="entry name" value="S21p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01181">
        <property type="entry name" value="RIBOSOMAL_S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000266714" description="30S ribosomal protein S21">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="56"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="95" mass="10643" checksum="15C6030225AC7360" modified="2005-10-11" version="1">MQVLVRDNNVDQALKALKKKMQREGIFREMKLRGHYEKPSEKKAREKAEAVRRARKLARKKMQREGLLPMKPKPVFGAGPGAGRGGPAAGPRGPR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-06-12" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU01</accession>
    <name>NQOR_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD(P)H dehydrogenase (quinone)</fullName>
            <ecNumber evidence="1">1.6.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Flavoprotein WrbA</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">NAD(P)H:quinone oxidoreductase</fullName>
            <shortName evidence="1">NQO</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">Nwi_0978</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a quinone + H(+) + NADH = a quinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:46160"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.6.5.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a quinone + H(+) + NADPH = a quinol + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:46164"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.6.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="1">Binds 1 FMN per monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the WrbA family.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314279.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SU01"/>
    <dbReference type="SMR" id="Q3SU01"/>
    <dbReference type="STRING" id="323098.Nwi_0978"/>
    <dbReference type="EnsemblBacteria" id="ABA04240">
        <property type="protein sequence ID" value="ABA04240"/>
        <property type="gene ID" value="Nwi_0978"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0978"/>
    <dbReference type="eggNOG" id="COG0655">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051402_0_2_5"/>
    <dbReference type="OMA" id="LHFGMVI"/>
    <dbReference type="OrthoDB" id="796211at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050136">
        <property type="term" value="F:NADH dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008753">
        <property type="term" value="F:NADPH dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01017">
        <property type="entry name" value="NQOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010089">
        <property type="entry name" value="Flavoprotein_WrbA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037513">
        <property type="entry name" value="NQO"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01755">
        <property type="entry name" value="flav_wrbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000291020" description="NAD(P)H dehydrogenase (quinone)">
        <location>
            <begin position="1"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Flavodoxin-like" evidence="1">
        <location>
            <begin position="4"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="78"/>
            <end position="80"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="113"/>
            <end position="119"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01017"/>
        </source>
    </evidence>
    <sequence length="199" mass="20670" checksum="F368C6E9BDA049BD" modified="2005-10-11" version="1">MAKVLVLYYSAYGHIEAMANAVADGARQAGATVDVKRVPELVPEAVAKAKYFKLDQAAPLAKVDDLADYDAIVVGTGTRFGRMSSQMASFLDQAGGLWARGALHGKVGGAFSSSATQHGGQETTLFSIITNLLHFGMTIVGLNYGFAGQMGVKEVTGGAPYGATTIADGDGSRPPSENELAGARYQGRMIAETAAKLHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU15</accession>
    <name>Q3SU15_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TPR repeat and WD-40 repeat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0964</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314267.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0964"/>
    <dbReference type="EnsemblBacteria" id="ABA04226">
        <property type="protein sequence ID" value="ABA04226"/>
        <property type="gene ID" value="Nwi_0964"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0964"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026904_0_0_5"/>
    <dbReference type="OMA" id="CTLFRTR"/>
    <dbReference type="OrthoDB" id="1856290at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038740">
        <property type="entry name" value="BioF2-like_GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13480">
        <property type="entry name" value="Acetyltransf_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Acetyltransf_6" evidence="2">
        <location>
            <begin position="186"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="429"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="506" mass="57681" checksum="C388F21EA33D1371" modified="2005-10-11" version="1">MHIDLIEDMDTFSRLRENWDEVYDSDPYATLFLSWTWLLSWLEKLSTPWIVLAAKRSGSDAHYVAFFPLRIDTRIDGEGRLSNDLRMAGSHAADYCGLISIPDFESDAITAFAQQIKRLNWTDFVLGNFSGSDARFTKLVSCFSQSKFTISQMDRVNKTDQVDNALCPYVELPDDWDTYLETLSANTRQKLRRLLRVVDANSDYRITHATADTVATDLDTLLRFWEIKWRPRKGKLTDSLVRAHRAMLSRSFDRGLLFLPTLWAGDRPLAALAILVDERKKSFLFYITGRDEEFAGPQPGLILHGHSIRYAISCGMKRYDFLRGNERYKYSFGVNERRLHCYVVATRSGRNLGGRLDVRAIADALKEATDLHQEGKIDQAARAYRQILETDPRNEGALHRYGQLLARRGDHVAAKRMFKLLTRMRTDTYKPWLLLGQSCEAMGQFLEAANAYREVIRLQPAVPDIFSKLVNVLFKAGRIEDARQALMTSYGVGSTGAEGGASFSIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU20</accession>
    <name>Q3SU20_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0959</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314262.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0959"/>
    <dbReference type="EnsemblBacteria" id="ABA04221">
        <property type="protein sequence ID" value="ABA04221"/>
        <property type="gene ID" value="Nwi_0959"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0959"/>
    <dbReference type="eggNOG" id="ENOG50329ZU">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118105_0_0_5"/>
    <dbReference type="OMA" id="NHCIYSF"/>
    <dbReference type="OrthoDB" id="1466596at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="194" mass="22280" checksum="5B466C3E7EEA6126" modified="2005-10-11" version="1">MTASHLPQKPDFAALEAAAPETADRRTTVLSLIGSLVFNWSNNESLFIYVLMLLLKVDQVSAAIVFATLNTTRARLDLIQRLARVKIRDKALSKELDRVIDRFNDGTHIRNEFNHCMYTVDNTGVISHTQSMRLVQTRSALRFGEVKPLDDARIHEISEAVREMARINREIWDFLPRLEDHLRDREPTSNDLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU60</accession>
    <name>Q3SU60_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0919</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04181">
        <property type="protein sequence ID" value="ABA04181"/>
        <property type="gene ID" value="Nwi_0919"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0919"/>
    <dbReference type="HOGENOM" id="CLU_2480205_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="87" mass="9499" checksum="40E73D8DDD53A9A8" modified="2005-10-11" version="1">MSLVSPLRLLAAISPSVNLQEINSSKPAKFLLKPFRSSTPDFRGKLSSKSISSVVTDDGSVRRKMPRQVKSRSRNLASDSIRSGDPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU62</accession>
    <name>Q3SU62_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cobalamin synthesis protein, CobW</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0917</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIMIBI class G3E GTPase family. CobW subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314220.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0917"/>
    <dbReference type="EnsemblBacteria" id="ABA04179">
        <property type="protein sequence ID" value="ABA04179"/>
        <property type="gene ID" value="Nwi_0917"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0917"/>
    <dbReference type="eggNOG" id="COG0523">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017452_0_0_5"/>
    <dbReference type="OMA" id="MWGVLRY"/>
    <dbReference type="OrthoDB" id="1267701at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011629">
        <property type="entry name" value="Cbl_biosynth_CobW-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036627">
        <property type="entry name" value="CobW-likC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07683">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00833">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobW C-terminal" evidence="3">
        <location>
            <begin position="254"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="223"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="350" mass="39269" checksum="F08AA37E802AD40E" modified="2005-10-11" version="1">MSDVNASASTKIPVTVLTGYLGAGKTTLLNRILSENHGKKYAVIVNEFGEVGIDNDLIIGADEEVFEMNNGCVCCTVRGDLVRILEGLMKRKGKFDAIIVETTGLADPAPVAQTFFVDEDVQNNARLDAVVTVADAKWLSERLKDAPEAKNQIAFADVIVLNKTDLVSQPELAEVEARIRGINPYAKLHRTERCQVALSDVLERGAFDLDRILEIEPDFLENDGHDHDHHDHHGHDHHDHDHGHGPKHYHDEEMQSLSLRSDKPLDQEKFMPWLQELLAAEGQKILRSKGLMAFSGDDDRYVFQGVHMMLEGSRQRAWKPDEKRESRLVFIGRELPEALIREGFQNCIAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUI0</accession>
    <name>Q3SUI0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0796</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314109.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0796"/>
    <dbReference type="EnsemblBacteria" id="ABA04061">
        <property type="protein sequence ID" value="ABA04061"/>
        <property type="gene ID" value="Nwi_0796"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0796"/>
    <dbReference type="eggNOG" id="COG5448">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114534_0_0_5"/>
    <dbReference type="OMA" id="EQRNINW"/>
    <dbReference type="OrthoDB" id="2005986at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011740">
        <property type="entry name" value="DUF2460"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09343">
        <property type="entry name" value="DUF2460"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02217">
        <property type="entry name" value="chp_TIGR02217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="196" mass="21279" checksum="3BD78735551FAE4C" modified="2005-10-11" version="1">MPQPFLDIAFPGSVGRGATGGPGFSTQIVTLASGAEQRNVNWSNSRGRWNISTGIRNRADMAAVIAHFHVVKGRAYSFRFKDWNDYDAADQAMVQNTPTVWQIVKRYNRSGYEHVRTITKPVAGSVAVKIAGSPVTPAAIDTLTGRITFASAPGSAPTASFQFDVPVRFDTDSLPVQANAWDLQIVNNIDLVEVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUQ5</accession>
    <name>Q3SUQ5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Assimilatory nitrite reductase (NAD(P)H) small subunit</fullName>
            <ecNumber evidence="6">1.7.1.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_0720</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.7.1.4" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314040.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0720"/>
    <dbReference type="EnsemblBacteria" id="ABA03986">
        <property type="protein sequence ID" value="ABA03986"/>
        <property type="gene ID" value="Nwi_0720"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0720"/>
    <dbReference type="eggNOG" id="COG2146">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055690_5_1_5"/>
    <dbReference type="OMA" id="KCPHKGG"/>
    <dbReference type="OrthoDB" id="1681886at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008942">
        <property type="term" value="F:nitrite reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042128">
        <property type="term" value="P:nitrate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012748">
        <property type="entry name" value="Rieske-like_NirD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13806">
        <property type="entry name" value="Rieske_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="SSF50022"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02378">
        <property type="entry name" value="nirD_assim_sml"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0534">Nitrate assimilation</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="5">
        <location>
            <begin position="3"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA03986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="109" mass="11501" checksum="88E79DB085E3455E" modified="2005-10-11" version="1">MSWIAIGGTEDIPLRGARCVATPLGRIAVFRTVENRFFAIEDRCPHKGGPLSQGIVHGAQVTCPLHGWVISLETGKAQGADEGSVRTFALKVEGGKLLISSDALVSMTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUY8</accession>
    <name>Q3SUY8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Enoyl-CoA hydratase</fullName>
            <ecNumber evidence="2">4.2.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0636</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.17" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313963.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0636"/>
    <dbReference type="EnsemblBacteria" id="ABA03903">
        <property type="protein sequence ID" value="ABA03903"/>
        <property type="gene ID" value="Nwi_0636"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0636"/>
    <dbReference type="eggNOG" id="COG1024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_2_5"/>
    <dbReference type="OMA" id="MSMTGDY"/>
    <dbReference type="OrthoDB" id="1498685at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="255" mass="27399" checksum="5CE2FFED539D1A2D" modified="2005-10-11" version="1">MTGHLQVSDNDATRVITMRRPEKKNAITRDMYRAMSDAIESAQNDPAIRCIVITGDSGMFTAGNDLDDFLKDGTSGDEDVAPAASDAVKFLYALARNAKPLIAAVEGIAIGIGATMLFHCDYVLAANSATFSTPFIHLGLVPEGASSLLAPRSIGYQRAFAMLVMGRTLSANEARESGFVNAVVAAGEAGAEAEKVAREICALPAEAVALSRKLLKTPPEELTRRIDLESRLFSERLRSEEAIAAFEAFFARKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV32</accession>
    <name>Q3SV32_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cystathionine gamma-synthase</fullName>
            <ecNumber evidence="4">2.5.1.48</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0592</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 3">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.48" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313920.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0592"/>
    <dbReference type="EnsemblBacteria" id="ABA03859">
        <property type="protein sequence ID" value="ABA03859"/>
        <property type="gene ID" value="Nwi_0592"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0592"/>
    <dbReference type="eggNOG" id="COG0626">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018986_2_0_5"/>
    <dbReference type="OMA" id="YKQDGVG"/>
    <dbReference type="OrthoDB" id="637281at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003962">
        <property type="term" value="F:cystathionine gamma-synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102028">
        <property type="term" value="F:cystathionine gamma-synthase activity (acts on O-phosphohomoserine)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011821">
        <property type="entry name" value="O_succ_thio_ly"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="PTHR11808"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02080">
        <property type="entry name" value="O_succ_thio_ly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="394" mass="41491" checksum="D3A9A9C3432C6B8D" modified="2005-10-11" version="1">MSESMNPRTVAAANGIAAEDTFGAVTPPICLSSTFTWPGFGRKGPHDYTRTSNPTRTLLADTLAKLEGGAGAVVVSSGMAAIDLVLSQLSSDDIVIVPHDCYGGTHRLLLNRRDRGHFKVRFVDQNDQAALASALKEKPRLVLIETPSNPLMRIVDIRAIVAQARTVGAKVAVDNTFLSPALQRPIALGADFVIHSTTKYLNGHSDIVGGAVIAADPADVEALETWANIVGTTGAPFDSFLALRGIRTLFPRVECQQRNAQAVAEFLLSHPLVAACHYPGLPSHPGHAVAKAQQSGFGAMLSFELKGGEVEAGRFVEAVRLFCFAESLGGTESLVAHPATMTHAGMSDEARKAAGITSGLIRLSIGLEDAADLIADLDQALAAIDRSVRSKASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="144" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV35</accession>
    <name>Q3SV35_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Chaperone protein ClpB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">clpB</name>
        <name evidence="15" type="ordered locus">Nwi_0589</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase activity; ATP hydrolysis unfolds the denatured protein aggregates, which probably helps expose new hydrophobic binding sites on the surface of ClpB-bound aggregates, contributing to the solubilization and refolding of denatured protein aggregates by DnaK.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homohexamer. The oligomerization is ATP-dependent.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homohexamer; The oligomerization is ATP-dependent.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the ClpA/ClpB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0589"/>
    <dbReference type="EnsemblBacteria" id="ABA03856">
        <property type="protein sequence ID" value="ABA03856"/>
        <property type="gene ID" value="Nwi_0589"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0589"/>
    <dbReference type="eggNOG" id="COG0542">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005070_1_0_5"/>
    <dbReference type="OMA" id="GPEHILM"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1780.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017730">
        <property type="entry name" value="Chaperonin_ClpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036628">
        <property type="entry name" value="Clp_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004176">
        <property type="entry name" value="Clp_R_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018368">
        <property type="entry name" value="ClpA/B_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028299">
        <property type="entry name" value="ClpA/B_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041546">
        <property type="entry name" value="ClpA/ClpB_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17871">
        <property type="entry name" value="AAA_lid_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02861">
        <property type="entry name" value="Clp_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81923">
        <property type="entry name" value="SSF81923"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03346">
        <property type="entry name" value="chaperone_ClpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51903">
        <property type="entry name" value="CLP_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00870">
        <property type="entry name" value="CLPAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00871">
        <property type="entry name" value="CLPAB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="Clp R" evidence="14">
        <location>
            <begin position="16"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="427"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00026057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABA03856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="891" mass="97966" checksum="9C88D42F6439BC08" modified="2005-10-11" version="1">MLSGQGRQAGETHMNIEKYTERVRGFIQSAQSLAMREGHQQFTPLHILKVLLDDSEGLAGSLIDRAGGNSRAILKATEAALDKMPKVSGAGAGQIYLAPATARAFDAAEQAAGKAGDSFVTVERLLQSLSLDADSDAFKLLKDGGVTPQNLNAAINALRKGRTADSATAENAYDALKKYARDLTQAAREGKLDPVIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIAEGLALRIVNGDVPESLKDKKLLALDLGSMIAGAKYRGEFEERLKAVLQEVTSADGGIVLFIDEMHTLIGAGKADGAMDASNLLKPALARGELHCVGATTLDEYRKHVEKDAALARRFQPIFVSEPTVEDTVSILRGLKDKYEQHHGVRIADSALVAAATLSHRYITDRFLPDKAIDLVDEAAARLKMQVDSKPEELDSLDREIVRLKIEQEALKKESDAGSRTRLENLEKELADLEKRSADLTSRWNAEKNKLSDAARLKSELDQARIELANAQRRGEYQIAGELAYGRIPELEKRLQSIEAGESNTMMNETVTADSIAQVVSRWTGVPVDKMLEGEKEKLLRMEESLAARVVGQGEAVRAVSTAVRRARAGLQDPNRPMGSFMFLGPTGVGKTELTKALAAYLFDDETAMVRIDMSEYMEKHSVARLIGAPPGYVGYDEGGALTEAVRRRPYQVVLFDEIEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFRNTLIIMTSNLGSEFLVNQPEGEDTGAVREQVMEMVRGHFRPEFLNRVDEIILFHRLQKSEMGRIVEIQFSRLQKLLEDRKIVLDLDSAARDRLAEKGWDPAYGARPLKRVIQRSVQDPLAEMILAGAVRDGDRVVISARDGVLTFNGEAPPAADIEPFSRPVPRRKLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV42</accession>
    <name>Q3SV42_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Prephenate dehydrogenase</fullName>
            <ecNumber evidence="2">1.3.1.12</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0582</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.3.1.12" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313910.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0582"/>
    <dbReference type="EnsemblBacteria" id="ABA03849">
        <property type="protein sequence ID" value="ABA03849"/>
        <property type="gene ID" value="Nwi_0582"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0582"/>
    <dbReference type="eggNOG" id="COG0287">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055968_0_1_5"/>
    <dbReference type="OMA" id="MWRDICL"/>
    <dbReference type="OrthoDB" id="533829at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008977">
        <property type="term" value="F:prephenate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004665">
        <property type="term" value="F:prephenate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003099">
        <property type="entry name" value="Prephen_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02153">
        <property type="entry name" value="PDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51176">
        <property type="entry name" value="PDH_ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prephenate/arogenate dehydrogenase" evidence="1">
        <location>
            <begin position="8"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="311" mass="33296" checksum="01DDF271D92561DB" modified="2005-10-11" version="1">MTAEPLFGRLALIGFGLIGGSIARAARMRGVVGEIVATARSAKTRARVMELGIVDRVVDTNAEATDGADLVILCVPVGACGSVAQQIAPHLKRGAIVSDVGSVKGAVVRDIAPHLPAEVHFVPAHPVAGTEHSGPDSGFAELFINRWCILTPPDGTDAAAVDRLRAFWTAIGARVEIMTPKHHDLVLAITSHLPHLIAYTIVGTADELGEVTSSEVMKFSAGGFRDFTRIAASDPTMWRDVFLTNKDAVLEMLGTFNEDLSKLTRAMRRGDGEALFEHFTRTRAIRRGIVEIGQDSAAPDFGRLQTDVQKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVC6</accession>
    <name>Q3SVC6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0498</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313826.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0498"/>
    <dbReference type="EnsemblBacteria" id="ABA03765">
        <property type="protein sequence ID" value="ABA03765"/>
        <property type="gene ID" value="Nwi_0498"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0498"/>
    <dbReference type="eggNOG" id="ENOG50331GU">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098924_0_0_5"/>
    <dbReference type="OMA" id="FVMLPQF"/>
    <dbReference type="OrthoDB" id="1777666at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="215" mass="22664" checksum="1124DCF6584637E5" modified="2005-10-11" version="1">MSVIGFPLLLIPLAICNIIVFLMPGVLLTEPVLTLTLMSGVSWTVTLGDTLLALGIVLLLFEIIKGVRPHGKYLTDHLLSLAVFGGAAAQFVVLPQFGSSTYFMLTLLALVDFLAGFALRARRPARGVLVPSQGMAEEVEAREPRAPSSSFMVAQASSQEPADPSAPAAEPLPERTIVHAETGLANSGYTETAGAKPHPDVPGPDIQQRDPGQAS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVE4</accession>
    <name>FOLD_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Bifunctional protein FolD</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="1">Methylenetetrahydrofolate dehydrogenase</fullName>
                <ecNumber evidence="1">1.5.1.5</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="1">Methenyltetrahydrofolate cyclohydrolase</fullName>
                <ecNumber evidence="1">3.5.4.9</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="1" type="primary">folD</name>
        <name type="ordered locus">Nwi_0480</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADP(+) = 5,10-methenyltetrahydrofolate + NADPH</text>
            <dbReference type="Rhea" id="RHEA:22812"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5,10-methenyltetrahydrofolate + H2O = (6S)-10-formyltetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="EC" id="3.5.4.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.5" evidence="1"/>
    <dbReference type="EC" id="3.5.4.9" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313811.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVE4"/>
    <dbReference type="SMR" id="Q3SVE4"/>
    <dbReference type="STRING" id="323098.Nwi_0480"/>
    <dbReference type="EnsemblBacteria" id="ABA03747">
        <property type="protein sequence ID" value="ABA03747"/>
        <property type="gene ID" value="Nwi_0480"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0480"/>
    <dbReference type="eggNOG" id="COG0190">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034045_2_1_5"/>
    <dbReference type="OMA" id="HIVGRPM"/>
    <dbReference type="OrthoDB" id="1449514at2"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004477">
        <property type="term" value="F:methenyltetrahydrofolate cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004488">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01080">
        <property type="entry name" value="NAD_bind_m-THF_DH_Cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01576">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000672">
        <property type="entry name" value="THF_DH/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020867">
        <property type="entry name" value="THF_DH/CycHdrlase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020631">
        <property type="entry name" value="THF_DH/CycHdrlase_NAD-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02882">
        <property type="entry name" value="THF_DHG_CYH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00085">
        <property type="entry name" value="THFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00766">
        <property type="entry name" value="THF_DHG_CYH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0486">Methionine biosynthesis</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000268419" description="Bifunctional protein FolD">
        <location>
            <begin position="1"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="166"/>
            <end position="168"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01576"/>
        </source>
    </evidence>
    <sequence length="294" mass="30340" checksum="A400F3728A1F6FA3" modified="2005-10-11" version="1">MAASIIDGKVIAADLRARVAGEVTRIKRDHGLTPGLAVVLVGNDPASEVYVRNKHKQTQAAGMASFEHMLPADVAQADVLALIAELNADPAVHGILVQLPLPKGLDTEAIIAAIDPAKDVDGLHPHNAGRLAGGLSALSPCTPLGCIILTKSVHASLEGLDAIVIGRSNLVGRPLVQLLLNENATVTIAHSRSRNLPELCRRADLVYAAVGRAEMVRGDWLKPGATVIDVGITRVPAAEGKTRLIGDVAFDEAMQVAGAVTPVPGGVGQMTVACLLVNTLRAACAIEGLSAPGV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-09-11" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVF4</accession>
    <name>TSAD_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA N6-adenosine threonylcarbamoyltransferase</fullName>
            <ecNumber evidence="1">2.3.1.234</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">N6-L-threonylcarbamoyladenine synthase</fullName>
            <shortName evidence="1">t(6)A synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaD</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA threonylcarbamoyladenosine biosynthesis protein TsaD</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tsaD</name>
        <name type="synonym">gcp</name>
        <name type="ordered locus">Nwi_0470</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosine(37) in tRNA + L-threonylcarbamoyladenylate = AMP + H(+) + N(6)-L-threonylcarbamoyladenosine(37) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:37059"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.3.1.234"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="1">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the KAE1 / TsaD family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.234" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313801.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVF4"/>
    <dbReference type="SMR" id="Q3SVF4"/>
    <dbReference type="STRING" id="323098.Nwi_0470"/>
    <dbReference type="EnsemblBacteria" id="ABA03737">
        <property type="protein sequence ID" value="ABA03737"/>
        <property type="gene ID" value="Nwi_0470"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0470"/>
    <dbReference type="eggNOG" id="COG0533">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023208_0_2_5"/>
    <dbReference type="OMA" id="HLEGHIY"/>
    <dbReference type="OrthoDB" id="1257362at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061711">
        <property type="term" value="F:N(6)-L-threonylcarbamoyladenine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01445">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017861">
        <property type="entry name" value="KAE1/TsaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022450">
        <property type="entry name" value="TsaD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="PTHR11735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF6">
        <property type="entry name" value="PTHR11735:SF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00789">
        <property type="entry name" value="OSIALOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="gcp_kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03723">
        <property type="entry name" value="T6A_TsaD_YgjD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000303454" description="tRNA N6-adenosine threonylcarbamoyltransferase">
        <location>
            <begin position="1"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="137"/>
            <end position="141"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01445"/>
        </source>
    </evidence>
    <sequence length="357" mass="36993" checksum="3D4FE65D7BB4250A" modified="2005-10-11" version="1">MLVLGIETTCDETAAAVVERLPDGSARILSNIVRSQTEEHAPYGGVVPEIAARAHVELLDGLIARAMTESGVGFRQLSGVAAAAGPGLIGGVIVGLTTAKAIALVHGTPLTAVNHLEAHALTPRLTSRLEFPYCLFLASGGHTQIVAVLGVGNYVRLGTTVDDAMGEAFDKVAKMLGLPYPGGPEVERAAASGDATRFNFPRPMLGRPDANFSLSGLKTAVRNEAARIDPLEPRDISDLCAGFQAAVLEATADRLGVGLRLFEERFGRPRALVAAGGVAANQAIRASLEGVAAKARTSLIIPPPALCTDNGAMIAWAGAERLAAGLTDSLETPPRARWLLDANAQAPAGFANTRAGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVR6</accession>
    <name>Q3SVR6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acyltransferase 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0358</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0358"/>
    <dbReference type="EnsemblBacteria" id="ABA03625">
        <property type="protein sequence ID" value="ABA03625"/>
        <property type="gene ID" value="Nwi_0358"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0358"/>
    <dbReference type="eggNOG" id="COG4763">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023915_5_1_5"/>
    <dbReference type="OMA" id="KVVHFAY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002656">
        <property type="entry name" value="Acyl_transf_3_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01757">
        <property type="entry name" value="Acyl_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="78"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="144"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="202"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="252"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="291"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="317"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl_transf_3" evidence="3">
        <location>
            <begin position="36"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="370" mass="41429" checksum="EE247D226C70CE0E" modified="2005-10-11" version="1">MKGERNRSSCKHARTRRKETPMTGNGTAAQTKERVDWVDYAKGICIIMVVMMHSVLGVEAAAGQTGFMHYVVEFAKPFRMPDFFLLSGLFLPLVINRDWRTYLDRKVVHFAYFYVLWVTIQFGFKAPAFAAEFGWPGVVRLWLMSFIDPFGTLWFIYLLPVFFVVIKATRRIPSWAVWGTAAALEMTHISTGWTLIDEFAARFVYIYSGYLFASYVFAWSNRARAYPGWTLAVIALWAVVNGGLVHTGISEWPIVSLALGLAGAGAIIAAGTLLAKMRWLDTLRYCGEHSIVIYLAFFLPMAASRTILLKAGLIQDIGLVSLIVTIAGVAGAVLIWWACRNTRANFLFERPDAFWIAPKKPAPTVQAAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVT5</accession>
    <name>Q3SVT5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Molybdenum transport system permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_0339</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Part of the binding-protein-dependent transport system for molybdenum; probably responsible for the translocation of the substrate across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 10">Cell inner membrane</location>
            <topology evidence="3 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0339"/>
    <dbReference type="EnsemblBacteria" id="ABA03606">
        <property type="protein sequence ID" value="ABA03606"/>
        <property type="gene ID" value="Nwi_0339"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0339"/>
    <dbReference type="eggNOG" id="COG4149">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_14_3_5"/>
    <dbReference type="OMA" id="MYSFIET"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015098">
        <property type="term" value="F:molybdate ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011867">
        <property type="entry name" value="ModB_ABC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02141">
        <property type="entry name" value="modB_ABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="31"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="106"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="220"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="34"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA03606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="253" mass="27283" checksum="95287F9CA5823069" modified="2005-10-11" version="1">MPQRRFTRSESQIIARQQLRDLMFDISTPEWMAVLLSLKVGVVATLVATPIAIALAWALARYEFWGKSIVDAAVHLPLVLPPVVTGYLLLLTFGKRGLVGGWLDDHLGIVFAFRWTGAALACGVMSFPLLVRPIRLSIEAIDRRLEQAASTLGASPWKVFATVTLPLALPGILAGMVLGFAKAIGEFGATITFVSNIPGETQTISSAIYSLIQTPDGDAAGMRLVIISIAMSMAALIASEVFTRRATRRLHGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVT9</accession>
    <name>Q3SVT9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">DNA repair protein RadC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_0335</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the UPF0758 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313667.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0335"/>
    <dbReference type="EnsemblBacteria" id="ABA03602">
        <property type="protein sequence ID" value="ABA03602"/>
        <property type="gene ID" value="Nwi_0335"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0335"/>
    <dbReference type="eggNOG" id="COG2003">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073529_0_0_5"/>
    <dbReference type="OMA" id="AMPDYEL"/>
    <dbReference type="OrthoDB" id="1833204at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08071">
        <property type="entry name" value="MPN_DUF2466"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025657">
        <property type="entry name" value="RadC_JAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001405">
        <property type="entry name" value="UPF0758"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020891">
        <property type="entry name" value="UPF0758_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30471">
        <property type="entry name" value="PTHR30471"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04002">
        <property type="entry name" value="RadC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00608">
        <property type="entry name" value="radc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01302">
        <property type="entry name" value="UPF0758"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MPN" evidence="7">
        <location>
            <begin position="114"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA03602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="236" mass="26102" checksum="CCCD45B8EAB1BC3F" modified="2005-10-11" version="1">MPANADNPSGFAEAPHYHGHRERLRERFRDAGPDALSDYELLEMVLFRALPRRDVKPLAKSLIGRFGSFAEVVHAPDARLREISGLGEAAIIEIKLIAATASRVAKGQLKQRTVLSSWTAVIDYCRTSMAFADKEQFRILFLDKRNQLIADEVQQVGTIDHTPVYPREVVKRALELSATAIIMVHNHPSGDPTPSQADIQMTKSIVDISSPLGISVHDHIIVGKNGHSSLKGLRLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVV7</accession>
    <name>Q3SVV7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Inner membrane-spanning protein YciB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">yciB</name>
        <name evidence="2" type="ordered locus">Nwi_0317</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in cell envelope biogenesis, maintenance of cell envelope integrity and membrane homeostasis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the YciB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0317"/>
    <dbReference type="EnsemblBacteria" id="ABA03584">
        <property type="protein sequence ID" value="ABA03584"/>
        <property type="gene ID" value="Nwi_0317"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0317"/>
    <dbReference type="eggNOG" id="COG2917">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089554_1_1_5"/>
    <dbReference type="OMA" id="AWVNFKL"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00189">
        <property type="entry name" value="YciB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006008">
        <property type="entry name" value="YciB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36917">
        <property type="entry name" value="PTHR36917"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04279">
        <property type="entry name" value="IspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00997">
        <property type="entry name" value="ispZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="201" mass="22372" checksum="74C9ED95622A139F" modified="2005-10-11" version="1">MMNKSQPHPLFKLATELGPLLVFFAANAKFGLFVATAAFMVAIVAAMIASYAVTRHIPLMALVTAVIVIVFGTLTLVLHDETFIKVKPTIIYALFAAVLGGGLLFGRSFIAIMFDQVFNLTPRGWRLLTLRWALFFAAMAVLNEFVWRTQSTDFWVNFKVFGAVPLTMIFAMMQMPLTRRHHLEPATLEASDAREGDVSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVW9</accession>
    <name>Q3SVW9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">T2SS-T3SS_pil_N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0305</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313638.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0305"/>
    <dbReference type="EnsemblBacteria" id="ABA03572">
        <property type="protein sequence ID" value="ABA03572"/>
        <property type="gene ID" value="Nwi_0305"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0305"/>
    <dbReference type="eggNOG" id="COG4964">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132581_0_0_5"/>
    <dbReference type="OMA" id="HFNATAS"/>
    <dbReference type="OrthoDB" id="1852588at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032789">
        <property type="entry name" value="T2SS-T3SS_pil_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13629">
        <property type="entry name" value="T2SS-T3SS_pil_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229302" description="T2SS-T3SS_pil_N domain-containing protein" evidence="1">
        <location>
            <begin position="33"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="T2SS-T3SS_pil_N" evidence="2">
        <location>
            <begin position="37"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="163" mass="17044" checksum="194700A55B901E46" modified="2005-10-11" version="1">MWFKFVRGRSRVSVIGVSFAAAILLGAATAPAEVISEPISVNVDQAKLVRLPGPIATIVVGNPLIADVTLQPGGLVVVTGKGYGATNMIAMDRAGSIMVDRVIQVEGPSDQVVTVYRGLERESYSCMPICQKRVTLGDSAAYFKATMDQASNLSSQAGGGATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVX6</accession>
    <name>Q3SVX6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Type II secretion system protein E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0298</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSP E family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313631.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0298"/>
    <dbReference type="EnsemblBacteria" id="ABA03565">
        <property type="protein sequence ID" value="ABA03565"/>
        <property type="gene ID" value="Nwi_0298"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0298"/>
    <dbReference type="eggNOG" id="COG4962">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005379_4_2_5"/>
    <dbReference type="OMA" id="HDERVIT"/>
    <dbReference type="OrthoDB" id="473670at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T2SP_E" evidence="3">
        <location>
            <begin position="132"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="488" mass="53528" checksum="17459388445142B2" modified="2005-10-11" version="1">MFGKRSGPEDNARKLASLTEVMPPPSNARASAEIASPKIASPPLAPPRPAAQTPSIDARRSDTYYQVKATIFGALIEAIDLAQLAKLDGESAREEIRDIVNEIIAIKNIVMSIAEQEELLDDICNDVLGYGPLEPLLSRDDIADIMVNGAGTVFIEVAGKIQRTGIRFRDNQQLLNICQRIVSQVGRRVDESSPICDARLADGSRVNAIVPPLAIDGPALTIRKFKKDKLTLDQLVRFGAITPEGGTILQIIGRCRANVLISGGTGSGKTTLLNCITNYIDHDERIITCEDAAELQLQQPHVVRLETRPPNIEGEGQITMRDLVRNCLRMRPERIIVGEVRGPEAFDLLQAMNTGHDGSMGTLHANNPREALSRCESMITMGGFALPSRTIREMICASIDIIIQAARLRDGSRRITHITELMGMEGDTIITQDIFVYDLLGEDANGNVIGRHRSTGIGRPRFWERARYYGEEKRLAAALDAAEVKVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW09</accession>
    <name>Q3SW09_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, XRE family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0265</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain fatty acyl-CoA assimilation regulator (ScfR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0265"/>
    <dbReference type="EnsemblBacteria" id="ABA03532">
        <property type="protein sequence ID" value="ABA03532"/>
        <property type="gene ID" value="Nwi_0265"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0265"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2856">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053651_0_1_5"/>
    <dbReference type="OMA" id="FTLAHYY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010359">
        <property type="entry name" value="IrrE_HExxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06114">
        <property type="entry name" value="Peptidase_M78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="2">
        <location>
            <begin position="23"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="400" mass="44826" checksum="3CCF5F58E2527209" modified="2005-10-11" version="1">MDAGKSMNGNSERLGDAEIGERLRIAREAAKLTQAEAAAAIGAARTTIVAIEQGKRRIQIDELQKLATSYRTSANAILRKEAVHLDMVPRFRKLSESSDHAVEQASRLLNDLVRAEVELENALGVARTRNYPPERPILPGDIRIQAEQDAQELRDWVGLGPGPVTDIVSILDLQLGIRVYVRRLDGKVAGLFAYDDAAGACILLNANHPHERLTQSGVHELAHFISTRRQPEVLTDDEPFVSREERYANIFQRCFLTPSRAVRQRFAEITAGQSHLTRRHIIFLAHAFGVSREAMVRRLEELKLAKDGTWDWFQSHGGITDEQAREVLGELPARPSSSVLARGLVPPRLALLAREAWKKQFYSEGQLARLLQLDRHEVREVLDGADIEESEADELFKLSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWA0</accession>
    <name>Q3SWA0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">OstA-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0173</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0173"/>
    <dbReference type="EnsemblBacteria" id="ABA03441">
        <property type="protein sequence ID" value="ABA03441"/>
        <property type="gene ID" value="Nwi_0173"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0173"/>
    <dbReference type="eggNOG" id="COG1934">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095993_0_0_5"/>
    <dbReference type="OMA" id="MQGFSQN"/>
    <dbReference type="OrthoDB" id="1696422at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005653">
        <property type="entry name" value="OstA-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03968">
        <property type="entry name" value="LptD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229297" evidence="2">
        <location>
            <begin position="40"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="OstA-like_N" evidence="3">
        <location>
            <begin position="62"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="227" mass="23992" checksum="D9BE3663FAEF6051" modified="2005-10-11" version="1">MISAVQNRRRFFLPAFRMAVLLRGGVVAVALMAAGEAAAQSTVQGVPNAMQGFSQNRDQPIQIEAATLEMRDKKKEATFSGNVKVVQGDTTMTSKTLVVYYQSSDTAKGGAPSKKKPAAVAAPAPSGASSIKRLEARGNVVVTQKDQVVTGDTAVFDTTTNLITMQGKVVLTQCKNVLRGDRLLVDMTTGVSRVESNSGRVQGLFIQGDQDCASPGPAIPLAPKKPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWB4</accession>
    <name>Q3SWB4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cytochrome c, class I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_0159</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0159"/>
    <dbReference type="EnsemblBacteria" id="ABA03427">
        <property type="protein sequence ID" value="ABA03427"/>
        <property type="gene ID" value="Nwi_0159"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0159"/>
    <dbReference type="eggNOG" id="COG2863">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076280_0_1_5"/>
    <dbReference type="OMA" id="AGCHSPN"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00034">
        <property type="entry name" value="Cytochrom_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome c" evidence="5">
        <location>
            <begin position="56"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="5">
        <location>
            <begin position="153"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA03427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="256" mass="26873" checksum="423E319EE474A444" modified="2005-10-11" version="1">MIPTRRAAPAQSGNRFSDKIMLHQKVKARAFSSRTKRMRSVAGGALLLLGVSSLPALADDGKGITRPPGRATSCTPCHGRNGEGQPVRGIPRLAGLNADYFERQLASFKNATRRNRIMLMIASDLSDDESRALASYYAGMSTPKPQTAPADRAVIAAGAELAANGDPSKDLQGCGQCHGADGLGAGADFPRLAGQSALYIEKALRSWKAGDRKNDPGGVMADVANKLTDDQIKSVAAYYESLPIAAAAPAPEANKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWD1</accession>
    <name>Q3SWD1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0142</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03410">
        <property type="protein sequence ID" value="ABA03410"/>
        <property type="gene ID" value="Nwi_0142"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0142"/>
    <dbReference type="HOGENOM" id="CLU_2383209_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="94" mass="10513" checksum="BE97405FEE2B9977" modified="2005-10-11" version="1">MRQRNARRGIGGIESRSETAGPTSSGPIHDLADSQPRVLTPLPGNGDRIYLLCFDAFSPREPDSIPLETLPSRQTGFRFLKVQPESAAWRQHWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWG4</accession>
    <name>Q3SWG4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Smr protein/MutS2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0109</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313446.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0109"/>
    <dbReference type="EnsemblBacteria" id="ABA03377">
        <property type="protein sequence ID" value="ABA03377"/>
        <property type="gene ID" value="Nwi_0109"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0109"/>
    <dbReference type="eggNOG" id="COG2840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055978_2_0_5"/>
    <dbReference type="OMA" id="HIGHGGE"/>
    <dbReference type="OrthoDB" id="1888882at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002625">
        <property type="entry name" value="Smr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036063">
        <property type="entry name" value="Smr_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01713">
        <property type="entry name" value="Smr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160443">
        <property type="entry name" value="SSF160443"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50828">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Smr" evidence="2">
        <location>
            <begin position="101"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="192" mass="21678" checksum="59A24CC65920AEE1" modified="2005-10-11" version="1">MKRFSPDASARPRRRRVLTDEERRLWESVAGQAKPLRKPSPPDSFQPRLEVKSASIAEFATRSESDPTGKAVRFNNPPRAALTRRERSQLSRGRKDIDARIDLHGMTQTRAYRALLRFLQDASGDGLIFVLVITGKGRAKGADSERGVLRRQVPQWLALPEFRSLVVGFDEAHLGHGGEGALYVRLRRRVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWK0</accession>
    <name>Q3SWK0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Thioredoxin-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0073</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0073"/>
    <dbReference type="EnsemblBacteria" id="ABA03341">
        <property type="protein sequence ID" value="ABA03341"/>
        <property type="gene ID" value="Nwi_0073"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0073"/>
    <dbReference type="eggNOG" id="COG3118">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046120_1_1_5"/>
    <dbReference type="OMA" id="QPRNMSM"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="33"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="336" mass="35706" checksum="C2A09B3095BC996B" modified="2005-10-11" version="1">MLAKHRRTSDNGTRPLIPGTSGEDHVTIVEHDGGPPPQAPDLINETTTQTFMKDVVEESMHQPVLVDFWAPRSGPSRQLSPLLEKAVRAAAGKVKLAKMNIDQHPAIFQQLAAQIGSHSIPAVFAFVGGRPVDYFTGAVPESQVKDFIDKLTQGAGAAPGAPNIEEILQEADAALAAGDPATAAAVYAEALGIDAANLRAIAGLARCYASTGAIDKAKQTLALVPESKRGDAAVTTVQAMIDLAEQASSLGPIAELEQKVAADPLDHQARFDLATALNAGGKRSEATDHLLEIVKRDRKWNDDAARKQLVQFFEAWGATDEATVEGRKRLSTILFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWL1</accession>
    <name>Q3SWL1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0062</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0062"/>
    <dbReference type="EnsemblBacteria" id="ABA03330">
        <property type="protein sequence ID" value="ABA03330"/>
        <property type="gene ID" value="Nwi_0062"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0062"/>
    <dbReference type="eggNOG" id="ENOG502Z9N4">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067303_0_0_5"/>
    <dbReference type="OMA" id="HWADKQK"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024453">
        <property type="entry name" value="Peptidase_C92"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05708">
        <property type="entry name" value="Peptidase_C92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="251"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="334" mass="36990" checksum="634F77C2AA6739B7" modified="2005-10-11" version="1">MGLVLDTVGKWIAAYLQKEIPGYRPFAPSDPEHLRDLIEEGDVLLVEGNSRISGIIKYLTQSTWSHSALYVGPIDGASEADGEPHVLIEANVGEGVVSAPLSKYFPYHSRVCRPVGLSHEDRVTVCRYAINRIGFGYDTKNIIDLMRYLVPLPFPQRWRRRMIAFGSGDPTKMICSALIAQAFGAVRYPVLPKITRAGSRSARREILHIRDSSLYMPRDFDISPYFEIVKPTIARGFDYRSLHWADTPKPLAESVHGEDPFQAGAGAPSFDPAPHDSPVTVEEVSAEESVPPRAQSETGDTAHIRGARARSPRRASGARPTHTGATFHHERSPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMZ7</accession>
    <name>Q3SMZ7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_3097</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06344">
        <property type="protein sequence ID" value="ABA06344"/>
        <property type="gene ID" value="Nwi_3097"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3097"/>
    <dbReference type="HOGENOM" id="CLU_3138273_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="49" mass="4998" checksum="54A5C50FBA69C665" modified="2005-10-11" version="1">MATRSSSSSMTCAFWVVDLGPVAGEDGGRVIARGIPCEIADAKDSLTAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN09</accession>
    <name>Q3SN09_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_3085</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3085"/>
    <dbReference type="EnsemblBacteria" id="ABA06332">
        <property type="protein sequence ID" value="ABA06332"/>
        <property type="gene ID" value="Nwi_3085"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3085"/>
    <dbReference type="eggNOG" id="ENOG502ZCM1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2586159_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="80" mass="8742" checksum="3F066A7D7D9B171E" modified="2005-10-11" version="1">MRDDGTGDPERLIQFVKRCATEFGLTGRWGFQYADTCSRPRLDGFGGGAHVIDLTTGGTVAWIYTDGWLAEVLDGDDPDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN47</accession>
    <name>Q3SN47_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FecR protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_3044</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316210.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3044"/>
    <dbReference type="EnsemblBacteria" id="ABA06294">
        <property type="protein sequence ID" value="ABA06294"/>
        <property type="gene ID" value="Nwi_3044"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3044"/>
    <dbReference type="eggNOG" id="COG3712">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050192_0_1_5"/>
    <dbReference type="OMA" id="PRHALAW"/>
    <dbReference type="OrthoDB" id="1381035at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006860">
        <property type="entry name" value="FecR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032623">
        <property type="entry name" value="FecR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012373">
        <property type="entry name" value="Ferrdict_sens_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30273">
        <property type="entry name" value="PTHR30273"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16220">
        <property type="entry name" value="DUF4880"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04773">
        <property type="entry name" value="FecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018266">
        <property type="entry name" value="FecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4880" evidence="2">
        <location>
            <begin position="20"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="FecR" evidence="1">
        <location>
            <begin position="112"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="318" mass="34625" checksum="FF37C8D602C635C7" modified="2005-10-11" version="1">MERGADSQMADDPDHVRIVEEAAVWFATMHDGKPSAADTKALKAWLAADPRHVRAYADIDRLWTGAIELPGMKDRHRAARKAVTRRRFGEAVVIGAVGLGAWRYLASHPFADYRTATGERRTVNLADGSTVDLAAETKLSLAFTRERRGLTLHEGEAFFTVAKDAGRPFVVEAGEGRTTALGTAFGVDYRDDSATVIVTDSTVDVALGEESARVSAGSLVTYDSRHIGELRQSETGTELAWREGRLVFTQAPLGQAVQALNRWRSGRIVVMSTSLAQRPITLIVNLNRTETIVAQLAEEVPIRIVAATPYLTLLFPAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN57</accession>
    <name>Q3SN57_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pyridoxamine 5'-phosphate oxidase-related, FMN-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_3034</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316202.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3034"/>
    <dbReference type="EnsemblBacteria" id="ABA06284">
        <property type="protein sequence ID" value="ABA06284"/>
        <property type="gene ID" value="Nwi_3034"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3034"/>
    <dbReference type="eggNOG" id="COG3576">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085054_0_0_5"/>
    <dbReference type="OMA" id="CDVSPKG"/>
    <dbReference type="OrthoDB" id="1885467at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024029">
        <property type="entry name" value="Pyridox_Oxase_FMN-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="Putative_PNPOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04025">
        <property type="entry name" value="PPOX_FMN_DR2398"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Putative_PNPOx" evidence="1">
        <location>
            <begin position="30"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="204" mass="22577" checksum="E34D950242FA4785" modified="2005-10-11" version="1">MVTAIETVEQLEAIYGQPGEAATIKVAYRITPAYRALIESSPFAILATCGEEGLDCSPRGDLPGFVRVQDDLTLILPDRRGNNRIDSLRNLIRDPRAALLFLIPGSGTTVRVNGRALVSADPELLASFGVEGKTPRSVIVMTVQEIYFQCARAIIRSHLWDPSRHVDLKSLPTPGEILAEMSKDRVGGEEYDKAWLERARQTMW</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN65</accession>
    <name>Q3SN65_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Trehalose 6-phosphate phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_3026</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Removes the phosphate from trehalose 6-phosphate to produce free trehalose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>alpha,alpha-trehalose 6-phosphate + H2O = alpha,alpha-trehalose + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23420"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16551"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58429"/>
            <dbReference type="EC" id="3.1.3.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1 3">Glycan biosynthesis; trehalose biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the trehalose phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.12" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3026"/>
    <dbReference type="EnsemblBacteria" id="ABA06276">
        <property type="protein sequence ID" value="ABA06276"/>
        <property type="gene ID" value="Nwi_3026"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3026"/>
    <dbReference type="eggNOG" id="COG1877">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037265_2_0_5"/>
    <dbReference type="OMA" id="YGAERWD"/>
    <dbReference type="UniPathway" id="UPA00299"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044651">
        <property type="entry name" value="OTSB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43768">
        <property type="entry name" value="PTHR43768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00685">
        <property type="entry name" value="T6PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA06276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="269" mass="28856" checksum="C9B9FA646E8077B9" modified="2005-10-11" version="1">MNQGVTGTVPNKTASVADDVEPRTLPLGTSALLLDIDGTLLDLAPSPSEVRVPPRLASSLPRLLSRTGGALALVSGRSLRDIDRIFAPTLFPAVGGHGAEMRISVESEAVAAHAPPMNPDLMRRFAAISDLHPGILVENKGYSLALHFRKAPDVELAIYDRVSAIRAELPNAPIEVLPGKFVCEIKHSGFTKATGVAELMSHEPFKGRRPVFIGDDVTDETVFAIMPDMDGLSFSVGRRAQGVDGHFQAPCNVRAWLARLLDDERAIAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN87</accession>
    <name>Q3SN87_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TPR repeat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_3004</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316176.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3004"/>
    <dbReference type="EnsemblBacteria" id="ABA06254">
        <property type="protein sequence ID" value="ABA06254"/>
        <property type="gene ID" value="Nwi_3004"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3004"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_285103_0_0_5"/>
    <dbReference type="OMA" id="WRESDFR"/>
    <dbReference type="OrthoDB" id="1105892at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="AAA_16" evidence="2">
        <location>
            <begin position="30"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="687"/>
            <end position="720"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1082" mass="118348" checksum="CAC4DC423E5651B5" modified="2005-10-11" version="1">MSVSVSSTIEEMQAERNALQSFADDRARDFVGRQSVLARVTDLCLSPAKELSPTKEGPPWGICITGDPGSGKSALFGELLRRLKETDAFILAHAAEATPQATSIDAMLRRWITELGSALGAGDIDLAANIDPEIVERAFASLLVRMASKRRVVVLIDAIDQFRKTSRGKFTSWLPRLWPINARLVATGVAGGASKALSERSSVEALALPPLESSEAEGIIEAVCKRNQRKLASPVIDALLAKKHAGGPAWGNPLWLVLALEELELLDSSDFADMQREYAGSPEQRLQSAMLDAVGAMPTDIPLLCRATFDSAAQLLSPAIAAAFIGLLSAGRTGWRESDFRQLLPQVGGEAWDQKRFATLRNLFRGQVHQHGDLAQLDFNHSQARAAARSRLAALRVTNPELHLLIADHLLTLAPDDRLRVTETMVHLLASKDDVRAARHYGDPSLGEKELGAATLALADAVISPATGTPASAAREMCRLLENPDHSVRARVAERLLVSFDDVAGRHIASDVRLIVLNAIEQAFRQLLRDEPGNAGWQRNLSVAHDRVGDVLVAQGKLPEALASFREGLAIREKLTGTDPDNAGLQHDLSISYEKIGDLLAVQGKLDEALDSIRKQLAVVERLANSDPDNADLQSEISLCHEKIGEALMARGDLPGALGAFQNQLEISERLARGADTDDSKWQRERTLAYDRVGDVLMAQGKLPEALEFFRNGLTIKERVAKAHPENTSWQRSLSISHDRIGDALVTQGKLPDALTSYRTGLEIAQKLARTEPENVGWQRDLSVSHAKIGDVLVAQSSLPDALKAFHEGLTIRQRLANADPDNVDWQRGLAVSYDRIGDVLMAQDNMGDALSAFQDQFAIAEKLARIDPNNTGWQRDLSVSYEKVGDVQMAQGNLPDALKSYRGGLAIRERLARTEPDNINWQRSLSVSHDCIGDVLEAQGEVDEALKAFHEGLEIRKRLAQRDPSNVGWQRDLTVSYDRLGEVLESKGDLEAAEKSFREGLAIMERLSLADPGNVDLQRGVAVSQGHLAEMYRRSNDHNSALAALRQGQAAMERVVMRAPENAGWKKDLDWFTEQISTLTD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-03-18" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNB4</accession>
    <name>AROE_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Shikimate dehydrogenase (NADP(+))</fullName>
            <shortName evidence="1">SDH</shortName>
            <ecNumber evidence="1">1.1.1.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aroE</name>
        <name type="ordered locus">Nwi_2977</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NADP(+) + shikimate = 3-dehydroshikimate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:17737"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the shikimate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.25" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316149.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SNB4"/>
    <dbReference type="SMR" id="Q3SNB4"/>
    <dbReference type="STRING" id="323098.Nwi_2977"/>
    <dbReference type="EnsemblBacteria" id="ABA06227">
        <property type="protein sequence ID" value="ABA06227"/>
        <property type="gene ID" value="Nwi_2977"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2977"/>
    <dbReference type="eggNOG" id="COG0169">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044063_2_0_5"/>
    <dbReference type="OMA" id="SIFARND"/>
    <dbReference type="OrthoDB" id="1054867at2"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00087"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004764">
        <property type="term" value="F:shikimate 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019632">
        <property type="term" value="P:shikimate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00222">
        <property type="entry name" value="Shikimate_DH_AroE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041121">
        <property type="entry name" value="SDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011342">
        <property type="entry name" value="Shikimate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013708">
        <property type="entry name" value="Shikimate_DH-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022893">
        <property type="entry name" value="Shikimate_DH_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006151">
        <property type="entry name" value="Shikm_DH/Glu-tRNA_Rdtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21089">
        <property type="entry name" value="PTHR21089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18317">
        <property type="entry name" value="SDH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01488">
        <property type="entry name" value="Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08501">
        <property type="entry name" value="Shikimate_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00507">
        <property type="entry name" value="aroE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000325138" description="Shikimate dehydrogenase (NADP(+))">
        <location>
            <begin position="1"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="21"/>
            <end position="23"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="132"/>
            <end position="136"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="156"/>
            <end position="161"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00222"/>
        </source>
    </evidence>
    <sequence length="279" mass="29886" checksum="A93AC62BD6AF701A" modified="2005-10-11" version="1">MMTAETTGRAACLIGWPAAHSRSPLIHHYWLRLHGITGGYSIEAIPPEGFAEFVMHLSTHGFAGANVTIPHKERALQLTEPDDRARAVGAANTLYYEGAVLRSTNTDVEGFISNLDVAAPSWDRTGDALVLGAGGASRAVVFGLVERGIGRIHLANRTVERARVLADQFGATVIPAAWNTLGDLLPRVGLLVNTTSLGMKGQPPLDIELALLPRDAVVADLVYVPLETPLLSAARARGLRTADGLGMLLHQAVRGFELWFGLRPAVTQELRALVEADLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNK6</accession>
    <name>Q3SNK6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, MarR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2885</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316065.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2885"/>
    <dbReference type="EnsemblBacteria" id="ABA06135">
        <property type="protein sequence ID" value="ABA06135"/>
        <property type="gene ID" value="Nwi_2885"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2885"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_35_2_5"/>
    <dbReference type="OMA" id="GVSRHYD"/>
    <dbReference type="OrthoDB" id="1744037at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="17"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="156" mass="17378" checksum="54A42EEADD4A8ADC" modified="2005-10-11" version="1">MRTFSDPTFETTILVRDTCLCLHAQRAARALARRFDDALKPVGLTNGQFSLLMSLNRPQPPNLGSVAALLAMDRTTLTANLKPLERRRLVETKADPKDGRARLLRLTPEGRAVLAEAVPIWRHLHAEIEKELSDPDHLRLELNVLSRSDKRGGVAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNQ7</accession>
    <name>Q3SNQ7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2831</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316024.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2831"/>
    <dbReference type="EnsemblBacteria" id="ABA06084">
        <property type="protein sequence ID" value="ABA06084"/>
        <property type="gene ID" value="Nwi_2831"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2831"/>
    <dbReference type="eggNOG" id="COG5471">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165535_1_0_5"/>
    <dbReference type="OMA" id="KTSAQAW"/>
    <dbReference type="OrthoDB" id="1773677at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011231">
        <property type="entry name" value="Phage_VT1-Sakai_H0018"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09956">
        <property type="entry name" value="DUF2190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF030771">
        <property type="entry name" value="UCP030771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="111" mass="11264" checksum="2E81021DB0B24ABF" modified="2005-10-11" version="1">MKNYVQPGNTITLTAPYAVVSGDGLLVGSVFGVAAGTAASGEPVETALVGVFDLTKVGSQAWTVGARVYWDDTNKRTTTVSTDNALIGVAVEAVASGAGDTIGRVRLNASF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNR7</accession>
    <name>Q3SNR7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ISBm1, transposase orfA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2821</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2821"/>
    <dbReference type="EnsemblBacteria" id="ABA06074">
        <property type="protein sequence ID" value="ABA06074"/>
        <property type="gene ID" value="Nwi_2821"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2821"/>
    <dbReference type="eggNOG" id="COG3293">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055261_2_6_5"/>
    <dbReference type="OMA" id="MEWIFID"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025161">
        <property type="entry name" value="Put_IS4/IS5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13340">
        <property type="entry name" value="DUF4096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="87" mass="10017" checksum="F6CF6F38E2FD4B88" modified="2005-10-11" version="1">MDDRRVLNGIFWRLRTGAPWADIPARYGPHTTCVNRFNRWRKAGVWDRILSAVSKAYDGDIQMIDSSSIRVHQHAANGQKNRRDPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNX9</accession>
    <name>Q3SNX9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sulfate transport system permease protein CysT</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_2759</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Part of the ABC transporter complex (TC 3.A.1.6.1) involved in sulfate/thiosulfate import.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The complex is composed of two ATP-binding proteins (CysA), two transmembrane proteins (CysT and CysW) and a solute-binding protein (CysP).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cell inner membrane</location>
            <topology evidence="2 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2759"/>
    <dbReference type="EnsemblBacteria" id="ABA06012">
        <property type="protein sequence ID" value="ABA06012"/>
        <property type="gene ID" value="Nwi_2759"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2759"/>
    <dbReference type="eggNOG" id="COG0555">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_14_0_5"/>
    <dbReference type="OMA" id="VFISGNM"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015419">
        <property type="term" value="F:ABC-type sulfate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011865">
        <property type="entry name" value="CysT_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005667">
        <property type="entry name" value="Sulph_transpt2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30406">
        <property type="entry name" value="PTHR30406"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30406:SF8">
        <property type="entry name" value="PTHR30406:SF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00969">
        <property type="entry name" value="3a0106s02"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02139">
        <property type="entry name" value="permease_CysT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0764">Sulfate transport</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="63"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="133"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="213"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="61"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA06012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="278" mass="30310" checksum="4785556E11FC2628" modified="2005-10-11" version="1">MKGGSGRRRTLPGFGLTMGLTLTWLSIIVLIPLAGLFLKSFELNFAQFVDIITSRRTLNALKISFGLAFLAALVNLVMGSIIVWALVRYRFPGRRIFDAIVDVPFALPTAVAGIALTTLFAQNGWLGAPLAELGINVSYTPLGIFLAMVFIGIPFVVRTVQPVLIDLDPEIEEAAASLGASRWQTVWRVVLPSLFPALLTGFALAFARAVGEYGSVIFIAGNLPNVSEIAPLLIVIRLSEFRYADATAIAVVMLLVSFLVIFVINRLQRWARAQEQAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP11</accession>
    <name>Q3SP11_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2727</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2727"/>
    <dbReference type="EnsemblBacteria" id="ABA05980">
        <property type="protein sequence ID" value="ABA05980"/>
        <property type="gene ID" value="Nwi_2727"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2727"/>
    <dbReference type="eggNOG" id="ENOG5030UZ1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1371004_0_0_5"/>
    <dbReference type="OMA" id="KTIWAVE"/>
    <dbReference type="OrthoDB" id="2123268at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229188" evidence="1">
        <location>
            <begin position="26"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="202" mass="21175" checksum="62420F4D322EAA27" modified="2005-10-11" version="1">MRITVALFVMTTIASLAVSASPANAAQNRFTSIANKDCTFAPIGNEPEDAEDQLKTCPGVGGAQVLVNSLGTRLRIGFRWPKAGQPQKTIWAVEAWSAGLTVNWRGSGTDNEFEPYAAIVRMKFQKQYLPVAGDQVLAVIRIAPDTACVMGAVDAGANPNANEAARALADAAPSFACGKDKPTIGGVSTRAAKEIARGVAEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP20</accession>
    <name>Q3SP20_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cell division and transport-associated protein TolA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2718</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315917.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2718"/>
    <dbReference type="EnsemblBacteria" id="ABA05971">
        <property type="protein sequence ID" value="ABA05971"/>
        <property type="gene ID" value="Nwi_2718"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2718"/>
    <dbReference type="eggNOG" id="COG3170">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_870628_0_0_5"/>
    <dbReference type="OMA" id="EMTFNPE"/>
    <dbReference type="OrthoDB" id="1767425at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019534">
        <property type="term" value="F:toxin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043213">
        <property type="term" value="P:bacteriocin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014161">
        <property type="entry name" value="Tol-Pal_TolA"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02794">
        <property type="entry name" value="tolA_full"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="350" mass="37282" checksum="1F924E70F1DBA61F" modified="2005-10-11" version="1">MRVKVDKTLAASVALHVLVIGWGLVSFSSKAFESVPEESLPVDIISADQLTHVTAGMKTGKKENPKPLVEKVAEPTPPPEDTVGKIDEKKPPVVTDVAPKEQPKPDDKAAAEAAAAAKAEAAEAKAKADAKAQAEAAAKAEAAKAQAEAVAEAKAQAEARAKAEAQAKAKAEARARAKAEAKAKADAKAKAEAKAKAEADAKAQAEAKRAERVFDRSKIAALLDKRDPSRHSVAGDTLNANAALGLAHGKAADNSATWGSMFKSQVERCWKKPYGGLEAQMTEAIFSIKLKRDGTLEATPTAISNPTTPYFRVYQESALRAIIECQPYKLPAAYFDEWKFFEPVFTERKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP32</accession>
    <name>Q3SP32_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Metal dependent phosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2706</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315905.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2706"/>
    <dbReference type="EnsemblBacteria" id="ABA05959">
        <property type="protein sequence ID" value="ABA05959"/>
        <property type="gene ID" value="Nwi_2706"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2706"/>
    <dbReference type="eggNOG" id="COG1896">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089999_0_0_5"/>
    <dbReference type="OMA" id="RWNGQTR"/>
    <dbReference type="OrthoDB" id="1508841at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="203" mass="22414" checksum="E8EBAF72A9F30176" modified="2005-10-11" version="1">MTARLPKTATRAWQRMLSGRRLDLLDPSPLDIEIADIAHGLARVARWNGQTHGAHIFSVAQHTLLVEAVMREHTPRIDIQVRLAALLHDAPEYVIGDMISPFKAVIGGSYKAVEKRLLAAIHIRFGLPPVLPPETGRQIKAADMGAAYLEATHLAGFSEPEAKRLFGNDPRLPAAMIDDYLTPWPASEAENRLLARFQSLHGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP48</accession>
    <name>Q3SP48_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2690</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315889.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2690"/>
    <dbReference type="EnsemblBacteria" id="ABA05943">
        <property type="protein sequence ID" value="ABA05943"/>
        <property type="gene ID" value="Nwi_2690"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2690"/>
    <dbReference type="eggNOG" id="COG3820">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081814_0_0_5"/>
    <dbReference type="OMA" id="KATAMWL"/>
    <dbReference type="OrthoDB" id="1621803at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010421">
        <property type="entry name" value="TrcR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06242">
        <property type="entry name" value="DUF1013"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="233" mass="25760" checksum="798A587E47FAE07F" modified="2005-10-11" version="1">MSNAPLMPKATAVWLVDNTALSFDQVADFTKMHPLEVRAIADGDAAQGIKGMDPISTGQLTRDEIEKGEKDPDYRLKLSESRVVLPPAAKKKGPRYTPVSRRHERPSAILWLVRNHPELKDAQIMRLVGTTKTTIAAVRDRTHWNASTLTPMDPVTLGLCSQIELDFEVQRAAKEKPTDQQYGGATLLPASETTRKDGGFEPTEKQQEDLNVDAVFAKLKTIGGKKAEDEQTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP74</accession>
    <name>Q3SP74_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF892 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2664</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315867.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2664"/>
    <dbReference type="EnsemblBacteria" id="ABA05917">
        <property type="protein sequence ID" value="ABA05917"/>
        <property type="gene ID" value="Nwi_2664"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2664"/>
    <dbReference type="eggNOG" id="COG3685">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102561_2_0_5"/>
    <dbReference type="OMA" id="FEMIGKP"/>
    <dbReference type="OrthoDB" id="1739824at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010287">
        <property type="entry name" value="DUF892"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05974">
        <property type="entry name" value="DUF892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF892" evidence="2">
        <location>
            <begin position="7"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="165" mass="18352" checksum="AC47BF16DFDF881E" modified="2005-10-11" version="1">MSKQKRLQDLFHDTLKDIYFAEKKILSALPKMAKAAQSDELRAAFEKHVDETEGQIERLEQVFEEIEARPQGKTCDAILGIIEEGKEIMSEYKGSPALDAGLLAAAQAVEHYEISRYGTLIAWANQLGFSNSAKLLKQTLVEEKKTDEALVTLAENIVNIEAEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP88</accession>
    <name>Q3SP88_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cytochrome c, class I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2650</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315853.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2650"/>
    <dbReference type="EnsemblBacteria" id="ABA05903">
        <property type="protein sequence ID" value="ABA05903"/>
        <property type="gene ID" value="Nwi_2650"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2650"/>
    <dbReference type="eggNOG" id="COG3474">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1160005_0_0_5"/>
    <dbReference type="OMA" id="YTADPQR"/>
    <dbReference type="OrthoDB" id="1505854at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00034">
        <property type="entry name" value="Cytochrom_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229197" evidence="5">
        <location>
            <begin position="22"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="6">
        <location>
            <begin position="12"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="6">
        <location>
            <begin position="139"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="225" mass="24069" checksum="120569E8BCC7BD92" modified="2005-10-11" version="1">MKGLLLAVAGLAAALLGSASAADKLDPSCSGCHALAKPADTSLDRLLTRKGPDLWFAGSKFKKEWLVAWLQDPKPIRPAGYPYFKTVKKGDKNDVPDPSKIAAHPKLKKADAEAATEALMALKAPADLVPEGIFKGEMKGARMGSLAFVKFRGCVACHQGEKGEGGLSGPRLTDAGARLQADFIAAYTADPQRFDPHIWMPTSNLKDQDIQRLTAFLVQLSQGDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPA4</accession>
    <name>Q3SPA4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metallophosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2634</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315837.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2634"/>
    <dbReference type="EnsemblBacteria" id="ABA05887">
        <property type="protein sequence ID" value="ABA05887"/>
        <property type="gene ID" value="Nwi_2634"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2634"/>
    <dbReference type="eggNOG" id="COG1409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052611_0_0_5"/>
    <dbReference type="OMA" id="NHDAYVR"/>
    <dbReference type="OrthoDB" id="1242748at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="1">
        <location>
            <begin position="5"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="301" mass="33419" checksum="5E181CB1900A2BCD" modified="2005-10-11" version="1">MTALTLAHLSDPHLGPLPPLRLHQLAGKRAIGYLNWKRGRHAIHRRDVLDALVADIKAQSPDHIAVTGDLINLALPDEFTQARAWLSQVGAPHNVTLVPGNHDAYVRPTQKQISEWEDYMRGDNAGEGTPLSFPFLRRRGPLTLIGASSSVPTRPFMATGRLGPKQLDALDRGLSQLESDPNFRVLLIHHPLSSKDSYKRLTDADALLDLLKRRGVNLILHGHDHIHSTMWFDGPQGRIPAIGVPSASAMPHRHYPPAAYNLFSITPDGNGWRCEQQVRGFADQAAIERKEVSELQRTQLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPE8</accession>
    <name>Q3SPE8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Methyltransferase small</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2590</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315793.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2590"/>
    <dbReference type="EnsemblBacteria" id="ABA05843">
        <property type="protein sequence ID" value="ABA05843"/>
        <property type="gene ID" value="Nwi_2590"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2590"/>
    <dbReference type="eggNOG" id="COG4123">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061983_1_1_5"/>
    <dbReference type="OMA" id="EAHAMTD"/>
    <dbReference type="OrthoDB" id="1027015at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071704">
        <property type="term" value="P:organic substance metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MTS" evidence="2">
        <location>
            <begin position="36"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="261" mass="26949" checksum="35F581C4A19FFE0E" modified="2005-10-11" version="1">MTCAQSDIGEDAFLGGRLRLYQPLSGHRAGHDAMLLAAATPGGPGDRIVDFGAGVGAAGLAVATRIDNIDLVLIERDETLAGLAGRNALLNRLAARVCVLDIAAEADAFAAAGLGPDSADGVLMNPPFNDAARHRPSPDQSRRAAHVADPSTLDIWTHAARRMLKSGGALSLIWRSDGLAEVLGALGRGFGGIAIRPVHPDPRRPAIRILVRAIKGSRAPLRLCPGLMLSDEMGQPDKEAQGSLAGGEALAFAPAGKGGFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPG2</accession>
    <name>Q3SPG2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2576</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315780.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05829">
        <property type="protein sequence ID" value="ABA05829"/>
        <property type="gene ID" value="Nwi_2576"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2576"/>
    <dbReference type="eggNOG" id="ENOG502ZSPU">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_836191_0_0_5"/>
    <dbReference type="OMA" id="IHFYTQW"/>
    <dbReference type="OrthoDB" id="2014930at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="237"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="265"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="288"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="320" mass="34103" checksum="24609E870649AB45" modified="2005-10-11" version="1">MKITLDISRLVEEGKLTSEEADRLTKLAAHDTASLGINILVGFAVVAIAAGAVALAPAPLTAIGFGLALFAAGFAIALNRVQHWMLLGQIFLVIGALMFGGGAIAYRADLLATMLIITGVFGLAAIIARSSLLMALAVLAASACLGARTGYSHASYSLAIYEPTLTVVVFSMVALIAYQASQRLPAEYARLAITAARTSLLLINFGFWIGSMWGDPLMLMRSLAARDLSSTLMTKPVIPSLVFSVLWAVALLGAGIWAVRANRRWLVNLVAVFSGIHFYTQWFEKLGATPFSVLLGGLVMLASAIALWMFNRRVADRDRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPI8</accession>
    <name>Q3SPI8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pseudouridine synthase</fullName>
            <ecNumber evidence="4">5.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2550</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a uridine in RNA = a pseudouridine in RNA</text>
            <dbReference type="Rhea" id="RHEA:48348"/>
            <dbReference type="Rhea" id="RHEA-COMP:12068"/>
            <dbReference type="Rhea" id="RHEA-COMP:12069"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the pseudouridine synthase RsuA family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315754.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2550"/>
    <dbReference type="EnsemblBacteria" id="ABA05803">
        <property type="protein sequence ID" value="ABA05803"/>
        <property type="gene ID" value="Nwi_2550"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2550"/>
    <dbReference type="eggNOG" id="COG1187">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_395711_0_0_5"/>
    <dbReference type="OMA" id="GPEKKFA"/>
    <dbReference type="OrthoDB" id="1037615at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120159">
        <property type="term" value="F:rRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042092">
        <property type="entry name" value="PsdUridine_s_RsuA/RluB/E/F_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000748">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluB/E/F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018496">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00093">
        <property type="entry name" value="TIGR00093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01149">
        <property type="entry name" value="PSI_RSU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S4" evidence="6">
        <location>
            <begin position="354"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="606"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="53"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="99"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="144"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="332"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="614"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="680" mass="76073" checksum="F7CC8477C10F2253" modified="2005-10-11" version="1">MPRDNDDDNARPRGRRDRPSGGKGKPRGRSGAPRGPEKKLARRGLAGKSDTGTSERGDGDKRPYAGKSDGAKPSGKKPYARKPHAGAGKPYAGKRDGGTARPRFNRDDGGEKRPYTPRGEGFRKDGDRPQRARPQGDRPYGARPPREGGGEKRPYTPRGDRPFGDKKTFSRGGPERGSRGPRKEFGDREGRNRGDEKPWQKRDAGPRDDRRGSRPSRDGAGRPRFSKSREDRVERAAGADRGPRKFDRPQGDRPRYDRRSDDRPRFDRPRQDRPDGDRSRGASNAWQERPRRFDRDDRPRRHDRDDRPRRDNEDDSKVFAKRPAFGGRGAYRERAPDGERRGARAEPKPKKAGERVAKMISRAGLASRRDAEEMVTQGRVAVNGRVINSPALDVTSRDVVTVDGQPLPERERTRLFLYHKPRGLMTTHADPEGRPTVFDHLPEGLPRVISVGRLDFNTEGLLLLTNDGGLARALELPDTGWLRRYRVRAHGEVTQAQLDALKNGVEVDGVKYGSIDATLERDQGANVWLTFAIREGKNREVRNVMAHLGLEVNRLIRISYGPFQLAEIAEGEVEEVKTRVLREQLGDRIATLANMDPSGAAAEAGAANLGRDGTPLRSKKDNKARTKRALIEDRQGRSVVVQRTGSDEARARNEAEANGYGPPRRPKRGYHGKRDLTPRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPI9</accession>
    <name>Q3SPI9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2549</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315753.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2549"/>
    <dbReference type="EnsemblBacteria" id="ABA05802">
        <property type="protein sequence ID" value="ABA05802"/>
        <property type="gene ID" value="Nwi_2549"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2549"/>
    <dbReference type="eggNOG" id="COG0742">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075826_1_1_5"/>
    <dbReference type="OMA" id="FNWLMPY"/>
    <dbReference type="OrthoDB" id="1827334at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031167">
        <property type="term" value="P:rRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004398">
        <property type="entry name" value="RNA_MeTrfase_RsmD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004553">
        <property type="entry name" value="CHP00095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00095">
        <property type="entry name" value="TIGR00095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="184" mass="19840" checksum="74C77132C5F0B594" modified="2005-10-11" version="1">MRVVGGRLKGRNLASPSTQQIRPTADRLRESLFNILVHAYDDPVADARVLDLFAGTGALGIEAVSRGAKFTLFVDNGAEARALLRNNVEALGLGGVTKVYRRDATNLGAAHPLAPFSLVFLDPPYRMQLADKALASLRDGGWLADGALLVVEEAKVAGFAAPDGFRELERRAYDDTEFVFLRAG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPJ0</accession>
    <name>MUTL_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA mismatch repair protein MutL</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mutL</name>
        <name type="ordered locus">Nwi_2548</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein is involved in the repair of mismatches in DNA. It is required for dam-dependent methyl-directed DNA mismatch repair. May act as a 'molecular matchmaker', a protein that promotes the formation of a stable complex between two or more DNA-binding proteins in an ATP-dependent manner without itself being part of a final effector complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutL/HexB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315752.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SPJ0"/>
    <dbReference type="SMR" id="Q3SPJ0"/>
    <dbReference type="STRING" id="323098.Nwi_2548"/>
    <dbReference type="EnsemblBacteria" id="ABA05801">
        <property type="protein sequence ID" value="ABA05801"/>
        <property type="gene ID" value="Nwi_2548"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2548"/>
    <dbReference type="eggNOG" id="COG0323">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004131_4_2_5"/>
    <dbReference type="OMA" id="AHERIMY"/>
    <dbReference type="OrthoDB" id="764332at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032300">
        <property type="term" value="C:mismatch repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00149">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014762">
        <property type="entry name" value="DNA_mismatch_repair_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020667">
        <property type="entry name" value="DNA_mismatch_repair_MutL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002099">
        <property type="entry name" value="DNA_mismatch_repair_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013507">
        <property type="entry name" value="DNA_mismatch_S5_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038973">
        <property type="entry name" value="MutL/Mlh/Pms"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014790">
        <property type="entry name" value="MutL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042120">
        <property type="entry name" value="MutL_C_dimsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042121">
        <property type="entry name" value="MutL_C_regsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037198">
        <property type="entry name" value="MutL_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073">
        <property type="entry name" value="PTHR10073"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01119">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08676">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01340">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00853">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118116">
        <property type="entry name" value="SSF118116"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00585">
        <property type="entry name" value="mutl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00058">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000010050" description="DNA mismatch repair protein MutL">
        <location>
            <begin position="1"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00149"/>
        </source>
    </evidence>
    <sequence length="603" mass="65288" checksum="04016129BCDBA83B" modified="2005-10-11" version="1">MPVRQLPETVVNRIAAGEVVERPASVVKELVENAIDAGATRIDVFTDGGGRRRIGVTDDGAGMAKGDLALAVDRHATSKLDDEDLLGIRTLGFRGEALPSIGAVARLCITTREAREPHAWSLTVEGGRKSEIIPAALTNGTRVEVGDLFYATPARLKFLKTDRTEAEAIREVVRRLAMARPDIGFTLAGEERAPVTWTAALPGAAGRLTRLGDILGGDFRAAAIPVRAEREGVIVEGFAAAPSLTRANALGQYLFVNGRPVRDKLILGAVRAAYSDYLPRDRHPVAALFVTADPREVDANVHPAKTEVRFRNAGLVRALIVHALKEGLAREGRRTAANRDGAVLTAFRPAPSPRPANWDWRESPAAPADARPWIGGPPAAAFAEAGQAALDVGAPSADVRFDAHPPLDLVDRPLGAARTQIHETYIVSQTRDGLIVVDQHAAHERIVYERLKTALARDGVQRQILLIPDIVELDEATVEKLIDRATELEKFGLAIESFGPGAVAVRETPSLLGKINAAALLRDLAEHMTEWEETLPLERRLMHVAATMACHGSVRAGRILKPEEMNALLREMEATPNSGQCNHGRPTYVELKLDDIEKLFGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPJ2</accession>
    <name>Q3SPJ2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Peptidase M16</fullName>
            <ecNumber evidence="6">3.4.24.64</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_2546</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M16 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.64" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2546"/>
    <dbReference type="EnsemblBacteria" id="ABA05799">
        <property type="protein sequence ID" value="ABA05799"/>
        <property type="gene ID" value="Nwi_2546"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2546"/>
    <dbReference type="eggNOG" id="COG0612">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009902_6_2_5"/>
    <dbReference type="OMA" id="WAFPAPE"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="SSF63411"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229215" evidence="3">
        <location>
            <begin position="31"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M16" evidence="4">
        <location>
            <begin position="49"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M16_C" evidence="5">
        <location>
            <begin position="198"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="445"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="464" mass="50066" checksum="7FC614E84C2BE986" modified="2005-10-11" version="1">MKRPGTGAARGLLAAVCFAVISFAAAPSFAATKIQHLVTPGGIEAWLVQDATVPLVAMEYAFAGGAAQDPPDKPGVGHLVASLLDEGAGDLSSKTFRERMERRAIELSFTIQRDRLRGSLRMLREHSGEAFGLLRLALTSPRFDADDVERIRSQILAHLRRNSTNPNALAGREFLKLAFGDHPYGHPSHGTLESVPTIRIDDLKDYVRRVVARDKLKITVVGDIEPAALAKMLDQTFGSLPAKGGLTPVPDIIAAKPPQKAFVPLDVPQTVVMFGGPGVKRHDPDFMAAYINNHILGGGSLSSRLYREVREKRGLAYSVSQSLLWMDHSALFAGTTGTRANRAGESIDAINSEIRRFAENGPTQKELDEAKSYVKGSQMLELDTSSKLATGLLQYQTDNLPIDYIEKRNAIVDAVTLDQAKAVAKRLWGQGLLTVIVGRSPQAVAQPAEAQPTATPPAPQPAAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPJ9</accession>
    <name>Q3SPJ9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HAD-superfamily subfamily IIA hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2539</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315743.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2539"/>
    <dbReference type="EnsemblBacteria" id="ABA05792">
        <property type="protein sequence ID" value="ABA05792"/>
        <property type="gene ID" value="Nwi_2539"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2539"/>
    <dbReference type="eggNOG" id="COG0647">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043473_2_0_5"/>
    <dbReference type="OMA" id="ILCDVWG"/>
    <dbReference type="OrthoDB" id="1308416at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006356">
        <property type="entry name" value="HAD-SF_hydro_IIA_hyp3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01460">
        <property type="entry name" value="HAD-SF-IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01459">
        <property type="entry name" value="HAD-SF-IIA-hyp4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="284" mass="31730" checksum="7D974934066F520F" modified="2005-10-11" version="1">MTTLRFIDHLRELAAERDVVLCDVWGVVHNGVMSFPEACSALKTFRDRGGAVIFITNAPRPADAVRRQLRRFGVPDDAYDGIASSGDLTRSYVAEHPAKAIFWLGPERDSAIHEGLDPVFAPIERADYIICTGPFDDETETAEDYRALMMQARERKLPLICANPDIVVQSGDRLLYCAGAIAELYRELGGEVVFYGKPHRPIYERAMALARERRGEDTPLSRVLAIGDSVRTDLMGAHAFGIDLVFLTRGIHSEQFAGIDQLDPTSVKELFGRPPRALMRELKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPS3</accession>
    <name>Q3SPS3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Sulfur carrier protein ThiS</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2465</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315673.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2465"/>
    <dbReference type="EnsemblBacteria" id="ABA05718">
        <property type="protein sequence ID" value="ABA05718"/>
        <property type="gene ID" value="Nwi_2465"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2465"/>
    <dbReference type="eggNOG" id="COG2104">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_174611_2_1_5"/>
    <dbReference type="OMA" id="RGKWDET"/>
    <dbReference type="OrthoDB" id="2084255at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010035">
        <property type="entry name" value="Thi_S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003749">
        <property type="entry name" value="ThiS/MoaD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34472">
        <property type="entry name" value="PTHR34472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02597">
        <property type="entry name" value="ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="SSF54285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01683">
        <property type="entry name" value="thiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="65" mass="7211" checksum="78A7637570A77094" modified="2005-10-11" version="1">MQVTVNGERREIAARTVDALLSELDYESTHVAIAVNFDVLPRSRWADTALRSGDEIEIITPRQGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPX2</accession>
    <name>Q3SPX2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2415</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2415"/>
    <dbReference type="EnsemblBacteria" id="ABA05669">
        <property type="protein sequence ID" value="ABA05669"/>
        <property type="gene ID" value="Nwi_2415"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2415"/>
    <dbReference type="eggNOG" id="ENOG50314AY">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2343859_0_0_5"/>
    <dbReference type="OMA" id="IGWVHER"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="97" mass="11369" checksum="73E3181F2E915E2C" modified="2005-10-11" version="1">MARGAIASLRIGWVHERKSLRGTLMDKPDNGKSDHDKSDNGYDYKRYRDLLAEATDESKRLELINLLIKEGARDQLMRERIRRLGLTKLVDRSWNRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPY4</accession>
    <name>Q3SPY4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Membrane protein involved in the export of O-antigen and teichoic acid</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2403</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2403"/>
    <dbReference type="EnsemblBacteria" id="ABA05657">
        <property type="protein sequence ID" value="ABA05657"/>
        <property type="gene ID" value="Nwi_2403"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2403"/>
    <dbReference type="eggNOG" id="COG2244">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055110_0_0_5"/>
    <dbReference type="OMA" id="ANIVFFM"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="203"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="237"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="276"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="317"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="346"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="371"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="419" mass="45540" checksum="83A02F5323833020" modified="2005-10-11" version="1">MVLRGATLASRFLLSILLARMLSPSDMGEYGLITAMLAFGLFVLGLEFYSYTLRELVPASPAKRVQIIADQIVLASASFAVISIVAVVAALMGLLPKKILFLFLLVLVTENASLEAMRILIITSRVVHAYIAGFLRGGIWVFALGLLMYLEPSTRSLEMVLIWWAGGGLLAVVYSALCLLDLPWRNLRCYRPDLGWIQAGLRTARPFMLTAVSALMLSYIDRFFIDGFVGRHELGIYTFYSTILIGILSLGTSISHQFLPRIISGHAVGADAYRRIVVLFLVSMLIVAGGLVVVAGLTMGPLLRLFGLRDYAEFIGVFYLMLPGLFLRMLADVPSYALYAAHSDKVLLLCNISAAAVTVVSNIVLVPIFGLAGAALSGAFASAILFLSLGVVARDRTRNYSGRPTETAAISNDTHARYN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ36</accession>
    <name>TYSY_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thymidylate synthase</fullName>
            <shortName evidence="1">TS</shortName>
            <shortName evidence="1">TSase</shortName>
            <ecNumber evidence="1">2.1.1.45</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thyA</name>
        <name type="ordered locus">Nwi_2351</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP = 7,8-dihydrofolate + dTMP</text>
            <dbReference type="Rhea" id="RHEA:12104"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="2.1.1.45"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; dTTP biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.45" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315569.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SQ36"/>
    <dbReference type="SMR" id="Q3SQ36"/>
    <dbReference type="STRING" id="323098.Nwi_2351"/>
    <dbReference type="EnsemblBacteria" id="ABA05605">
        <property type="protein sequence ID" value="ABA05605"/>
        <property type="gene ID" value="Nwi_2351"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2351"/>
    <dbReference type="eggNOG" id="COG0207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021669_0_0_5"/>
    <dbReference type="OMA" id="KQYLDLC"/>
    <dbReference type="OrthoDB" id="1212177at2"/>
    <dbReference type="UniPathway" id="UPA00575"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004799">
        <property type="term" value="F:thymidylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006231">
        <property type="term" value="P:dTMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00351">
        <property type="entry name" value="TS_Pyrimidine_HMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.572.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00008">
        <property type="entry name" value="Thymidy_synth_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045097">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023451">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036926">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000398">
        <property type="entry name" value="Thymidylate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020940">
        <property type="entry name" value="Thymidylate_synthase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11548">
        <property type="entry name" value="PTHR11548"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00303">
        <property type="entry name" value="Thymidylat_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00108">
        <property type="entry name" value="THYMDSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55831">
        <property type="entry name" value="SSF55831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03284">
        <property type="entry name" value="thym_sym"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00091">
        <property type="entry name" value="THYMIDYLATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000000642" description="Thymidylate synthase">
        <location>
            <begin position="1"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="126"/>
            <end position="127"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="166"/>
            <end position="169"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="207"/>
            <end position="209"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00008"/>
        </source>
    </evidence>
    <sequence length="264" mass="30027" checksum="C7A882962AC65F3A" modified="2005-10-11" version="1">MNQYHDLLERILGEGTEKHDRTGTGTLSVFGHQMRFDLASGFPMLTTKKLPFRSIVHELLWFLRGETNIKYLKDNGVSIWDEWADANGDLGPVYGSQWRSWPARDGRGIDQIANVIDLIQRNPDSRRLIVSAWNPADVDRMALPPCHCLFQFYVAGGKLSCQLYQRSADVFLGVPFNIASYALLTMMVAQVTGLKPGDFVHSLGDAHLYSNHLDQARLQLSRPTRPLPTMTINPEVKDIFAFRYEDFRLENYDPHPHIKAEVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ52</accession>
    <name>Q3SQ52_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315553.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2335"/>
    <dbReference type="EnsemblBacteria" id="ABA05589">
        <property type="protein sequence ID" value="ABA05589"/>
        <property type="gene ID" value="Nwi_2335"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2335"/>
    <dbReference type="HOGENOM" id="CLU_124387_1_0_5"/>
    <dbReference type="OrthoDB" id="1527784at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021322">
        <property type="entry name" value="DUF2924"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11149">
        <property type="entry name" value="DUF2924"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="155" mass="17200" checksum="7ADBC64DB9B6E910" modified="2005-10-11" version="1">MAEASKDGINRKRDLGGRRPQSRGRLSSWSALAHLPKHLQTAIVSYRRQADQSADLDPAIRQFLDRIVETQTPESIMAKLVAFDRGQVELKAGTILSREWNGITQRAIVVEDGFLWEGTRYDSLSAIAFAITGTRWSGPRFFGLRDSTVGGEDGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ80</accession>
    <name>Q3SQ80_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Outer membrane transport energization protein TonB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_1269</name>
        <name evidence="6" type="ordered locus">Nwi_2307</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="6">Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314558.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1269"/>
    <dbReference type="EnsemblBacteria" id="ABA04530">
        <property type="protein sequence ID" value="ABA04530"/>
        <property type="gene ID" value="Nwi_1269"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05561">
        <property type="protein sequence ID" value="ABA05561"/>
        <property type="gene ID" value="Nwi_2307"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1269"/>
    <dbReference type="KEGG" id="nwi:Nwi_2307"/>
    <dbReference type="eggNOG" id="COG0810">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076333_0_0_5"/>
    <dbReference type="OMA" id="PVIFRTK"/>
    <dbReference type="OrthoDB" id="1884140at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006260">
        <property type="entry name" value="TonB/TolA_C"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01352">
        <property type="entry name" value="tonB_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="56"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="85"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="124"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="263" mass="28023" checksum="C57D5229669486AA" modified="2005-10-11" version="1">MPEIVEQPPSGRLWLSAAAAALAIHLGCVALALAHLDTDEAAEDLGAPAIEIGLDLSSPHREVTDLPPGPDTDASMASPALAEQKAVEEETDLPKDTPTESADPDRIVTENESRKPFDDATEKAAKQTQASQESAAAEATAMPSVEQAQESERSVAPQQGTGASARRIRATWQKELVAHLDKHKRYPPDRSQKNAEILVNFELDRMGHVVSATIVNGSGDPIFDQAALAMLRRSDPVPPPPPLIADEGLSFTLPVVFRVKGKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQB2</accession>
    <name>Q3SQB2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Possible RND superfamily transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_2275</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2275"/>
    <dbReference type="EnsemblBacteria" id="ABA05529">
        <property type="protein sequence ID" value="ABA05529"/>
        <property type="gene ID" value="Nwi_2275"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2275"/>
    <dbReference type="eggNOG" id="COG1033">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008861_3_0_5"/>
    <dbReference type="OMA" id="SLMFAHI"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004869">
        <property type="entry name" value="MMPL_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03176">
        <property type="entry name" value="MMPL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="248"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="299"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="340"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="376"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="436"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="628"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="654"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="680"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="722"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="751"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="domain" description="MMPL" evidence="8">
        <location>
            <begin position="77"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="MMPL" evidence="8">
        <location>
            <begin position="467"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="797" mass="86506" checksum="F4F87F74E0420362" modified="2005-10-11" version="1">MGSRRNGQHMHERNPDAADMTKPDRRSLSIAFGFERVGLIAMRAPILSCIILLAMVVGAVFGIQRITIDDSLSQLFRSDTKEYRQYEEVTRRFPSTEFDVLVVVEGKTLLERDNLEKLRDLVTDLQLVDGVRGLISLFSARQAPEPGKLPAALFPAELPQGAEYDHFIETVKSNEIIRGKLLSEDGTLALVVLSLEPAIVSDSKLKATIGQIRQVMNDDLGDTGLTKELSGVPVMQLEIRNAVERDGIIYNVAGILAGCLIAIVFFRKVSFMVVAAFPPLLAILLSLGVLGWANFSLNMFLNVMTPLIMVISFSDSMQLTFAARDRLIAGEDKYSAFRNAVLVVGPACVLTHATAGISFIALQFSNSDLIRSFGEAGLLATIIALLAVLLLVPAFGVLLVRDERRFSEKFKTADWGVQALRRFCAWIAERMVGRPGFFSVLALLVVGGLAVVYASLEPRYRLADQVPDKEQAVAASGRLDAKLTGANPIDILIEFPAGETLYSPQTLATIAAVHKLVEEQAGVGNVWSIETLRRWLAEKAGSHDVATLKEYVSVIPEHLVRRFISEDQTAVVVSGRVPDRDSSQILPVVEKLDRAMDAVRTAHPGYEMAVTGLSAIAARNSASMIEKLNHGLTIEFVLVAVFIGLAFRSVVVMFACILPGIFPVVLSGTLLWLLGEGLQFASVVALTVSFGLGLSATIHFLNRLRSETGPGVGAGKAVERATVLMGPALILTTVVLACGLVVTVFSDLPSLRLFGWLSAFAMVAALVADFLILRPTAMWLITFAERFRHGAVAKPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQB6</accession>
    <name>Q3SQB6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Squalene/phytoene synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2271</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315492.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2271"/>
    <dbReference type="EnsemblBacteria" id="ABA05525">
        <property type="protein sequence ID" value="ABA05525"/>
        <property type="gene ID" value="Nwi_2271"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2271"/>
    <dbReference type="eggNOG" id="COG1562">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037269_1_1_5"/>
    <dbReference type="OMA" id="VKAPRIM"/>
    <dbReference type="OrthoDB" id="1449625at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004311">
        <property type="term" value="F:farnesyltranstransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016767">
        <property type="term" value="F:geranylgeranyl-diphosphate geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016117">
        <property type="term" value="P:carotenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00683">
        <property type="entry name" value="Trans_IPPS_HH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017828">
        <property type="entry name" value="SQ_synth_HpnD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002060">
        <property type="entry name" value="Squ/phyt_synthse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019845">
        <property type="entry name" value="Squalene/phytoene_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044843">
        <property type="entry name" value="Trans_IPPS_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033904">
        <property type="entry name" value="Trans_IPPS_HH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00494">
        <property type="entry name" value="SQS_PSY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01212">
        <property type="entry name" value="Phytoene_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="SSF48576"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03465">
        <property type="entry name" value="HpnD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01044">
        <property type="entry name" value="SQUALEN_PHYTOEN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01045">
        <property type="entry name" value="SQUALEN_PHYTOEN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="278" mass="31368" checksum="977C1285941D9497" modified="2005-10-11" version="1">MTIATTADASSGETASNSSFYAAMRILPRAQREAMFQIYTFCRYVDDIADSDRPRSERQAALQQWRDDIDALYQGRAPERLRDYVASVDAFGMRREDFLAVIDGMEMDVLADIRAPEEATLDLYCDRVASAVGRLSVRVFGLPEQDGILLSHHLGRALQLTNILRDLDEDAAIGRLYVPREELLRAGIDCTDPAAVISSPAIPEVCVRLMERARTHFVESDEIMNRNARRAVRAPRIMSKYYHAILDLLEERGFQPPRSPVRVSYAAKIAIVLRYALI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQC4</accession>
    <name>Q3SQC4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Aminotransferase</fullName>
            <ecNumber evidence="3">2.6.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2263</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315484.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2263"/>
    <dbReference type="EnsemblBacteria" id="ABA05517">
        <property type="protein sequence ID" value="ABA05517"/>
        <property type="gene ID" value="Nwi_2263"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2263"/>
    <dbReference type="eggNOG" id="COG4992">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_10_0_5"/>
    <dbReference type="OMA" id="PYRWPSG"/>
    <dbReference type="OrthoDB" id="572533at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="1 2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="463" mass="50694" checksum="ADF6FDC31A8A400D" modified="2005-10-11" version="1">MLYPNLDVSEMFVERQAQRSAMHVRHLNEQLVRVLKTIGYDVGFQSGQGQYLVDRDGARYLDLLSGFGVFAIGRNHPVLRHALKSVLDSDLPNLVQMDVSTLAGVLAERLLDHIPYLDKVFFANSGAESVEAAIKFARVATGRTGIVYCDHGYHGLTYGALSLTDDKNFRGGFDPLLPDCKVVPFNDLAALEQALSSRQIAALIVEPIQGKGVNMPTDEFLPGAAALCRKYGTLFVADEIQTGIGRTGRFLAVEHWNVEPDMVLLAKALSGGHVPIGVVLTRKHIFDKVFNRMDRAVVHGSTFAKNDLAMAAGIATLEVLKAENLIENAARRGAELQLALNRMVPGYELLKEVRGKGLMIGVEFGPPKSLRLRASWNLLETANKGLFCQLITVPLFKDHKILTQVAGHGSHTIKLLPPLIINDEDCKWIETSFDSVIAASHQVPGAIWSLGKTLMDNAVRKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQF7</accession>
    <name>Q3SQF7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_2230</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the glycosyltransferase 87 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2230"/>
    <dbReference type="EnsemblBacteria" id="ABA05484">
        <property type="protein sequence ID" value="ABA05484"/>
        <property type="gene ID" value="Nwi_2230"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2230"/>
    <dbReference type="eggNOG" id="ENOG5032WP4">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_637497_0_0_5"/>
    <dbReference type="OMA" id="NWSWVNQ"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018584">
        <property type="entry name" value="GT87"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09594">
        <property type="entry name" value="GT87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="146"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="197"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="290"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="343"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="372"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="395"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA05484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="430" mass="47723" checksum="3BBCF16906B9E21F" modified="2005-10-11" version="1">MPVLRWPSFRRPLDVLFLVACILLTADVLVPEIWGHGKTKDYPLWYWAGQQILHGGDLYPHGPNDGFAFLYPPLSAILLALPSAFGKLPLYLGLCLLNAAAWWMTAQLSNAMTGSGKTPDPWLEALPGLVTIPFVFDMFDLGQPNLVLLAMMLFGFWCLWRGRGWLSGGMFALAAAIKVFPVAVLPYLLWRRHWTSAISMVAFLGVLLFLAPAPIRGFDRNVSELTTWYRGMIGSNSEHGFGQRDAQNWSWVNQSIIAVTHRLVRPVNYNQIDPAKPAQYMNVANVDYRTANWIVLAVFAAIGLGFIAIIPARANMTQRSIAEEVGILFCLMTLASPLARQYYFVWLFFPITVLFHRAALDGRPKARAISRLALGASFALMALSLPVFPKILQAAGNNIAATFVMVAALAWHILNPPPARDPHSTAQPRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQI5</accession>
    <name>Q3SQI5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">VWA containing CoxE-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2202</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315425.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2202"/>
    <dbReference type="EnsemblBacteria" id="ABA05456">
        <property type="protein sequence ID" value="ABA05456"/>
        <property type="gene ID" value="Nwi_2202"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2202"/>
    <dbReference type="eggNOG" id="COG3552">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042261_0_1_5"/>
    <dbReference type="OMA" id="DFYWTLH"/>
    <dbReference type="OrthoDB" id="1121563at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011195">
        <property type="entry name" value="UCP010256"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008912">
        <property type="entry name" value="Uncharacterised_CoxE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05762">
        <property type="entry name" value="VWA_CoxE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010256">
        <property type="entry name" value="CoxE_vWa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="SSF53300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="2">
        <location>
            <begin position="213"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="408" mass="45239" checksum="5382484256BA6FA9" modified="2005-10-11" version="1">MAINHFDSPTGHIADNVAGFARTLRACGLPVGPGAVIDALKALRLIDIGNRADVFSTLQAIFMTRHDHAPIFAQAFDLFFCIAEEGKNMLDPVQPLDQARKPPPSASRRVLEALSRPAITSEREAPQGQEMRPSVSDLEVLQKKDFAQMSAAELAQVTQIIANMKLPQARLHTRRIRPDPRGSRLDLRRTLRGGLHTGGEIVDIHRLGRIDKPAPIVALLDISGSMSEYTRLFLHFLHGITNRRGRVSVFLFGTRLTNVTRALRARDPDEALAACSSVVEDWAGGTRIATSLHDFNKLWSRRVLGQGAVVLMITDGLEREAGSELAFEMDRLHRSCRRLIWLNPLLRYDGFEPRARGIKMMLPHVDEFRPVHNLSSIEGLIAALSQAPGRHGRGAARGLNEGHYARSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQI6</accession>
    <name>Q3SQI6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted sulfurylase small subunit, molybdopterin cytosine dinucleotide biosynthesis</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2201</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315424.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2201"/>
    <dbReference type="EnsemblBacteria" id="ABA05455">
        <property type="protein sequence ID" value="ABA05455"/>
        <property type="gene ID" value="Nwi_2201"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2201"/>
    <dbReference type="eggNOG" id="COG1975">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154250_0_0_5"/>
    <dbReference type="OMA" id="ETAWNVG"/>
    <dbReference type="OrthoDB" id="1843379at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003777">
        <property type="entry name" value="XdhC_CoxI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02625">
        <property type="entry name" value="XdhC_CoxI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XdhC_CoxI" evidence="1">
        <location>
            <begin position="15"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="107" mass="10923" checksum="6D1FD911060A5A92" modified="2005-10-11" version="1">MLDRDEDILKAAESWAEAGKGVALATVVETWGSAPRPAGSGLVVNEDGTFLGSVSGGCVEGAVVTEALDVISSGAPRMLEFGVADEAAWTVGLSCGGRIRVFVEKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQL7</accession>
    <name>Q3SQL7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transglutaminase-like cysteine peptidase, BTLCP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315393.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2170"/>
    <dbReference type="EnsemblBacteria" id="ABA05424">
        <property type="protein sequence ID" value="ABA05424"/>
        <property type="gene ID" value="Nwi_2170"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2170"/>
    <dbReference type="eggNOG" id="COG3672">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092032_1_0_5"/>
    <dbReference type="OMA" id="DHWGVVE"/>
    <dbReference type="OrthoDB" id="1500682at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010319">
        <property type="entry name" value="Transglutaminase-like_Cys_pept"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39327">
        <property type="entry name" value="PTHR39327"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06035">
        <property type="entry name" value="Peptidase_C93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="206" mass="22770" checksum="AB7E528DB7FAFFB1" modified="2005-10-11" version="1">MLNRGQGVGLAVVGAILWGIATSAGAAGAEKLYASLGDTTRAPIGWVEFCNDSPKECRPGATQPRDIVLTPDAWRDLLKVNRWVNETVKPMTDKDHWGVVEKWSLPTDGHGDCEDYVLLKRKMLIDAGWPREALLVTVVRDKQGDGHAVLTVKTDKGEFILDNQNENVMAWTKTGYRFMKRQSQSDPDIWVALDDNHPAVATASSR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-12-06" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQN7</accession>
    <name>RLME_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal RNA large subunit methyltransferase E</fullName>
            <ecNumber evidence="1">2.1.1.166</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">23S rRNA Um2552 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">rRNA (uridine-2'-O-)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rlmE</name>
        <name evidence="1" type="synonym">ftsJ</name>
        <name evidence="1" type="synonym">rrmJ</name>
        <name type="ordered locus">Nwi_2150</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-adenosyl-L-methionine + uridine(2552) in 23S rRNA = 2'-O-methyluridine(2552) in 23S rRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42720"/>
            <dbReference type="Rhea" id="RHEA-COMP:10202"/>
            <dbReference type="Rhea" id="RHEA-COMP:10203"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74478"/>
            <dbReference type="EC" id="2.1.1.166"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.166" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315373.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SQN7"/>
    <dbReference type="SMR" id="Q3SQN7"/>
    <dbReference type="STRING" id="323098.Nwi_2150"/>
    <dbReference type="PRIDE" id="Q3SQN7"/>
    <dbReference type="EnsemblBacteria" id="ABA05404">
        <property type="protein sequence ID" value="ABA05404"/>
        <property type="gene ID" value="Nwi_2150"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2150"/>
    <dbReference type="eggNOG" id="COG0293">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009422_4_0_5"/>
    <dbReference type="OMA" id="HRQTDHL"/>
    <dbReference type="OrthoDB" id="1798571at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008650">
        <property type="term" value="F:rRNA (uridine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01547">
        <property type="entry name" value="RNA_methyltr_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015507">
        <property type="entry name" value="rRNA-MeTfrase_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005461">
        <property type="entry name" value="23S_rRNA_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000155514" description="Ribosomal RNA large subunit methyltransferase E">
        <location>
            <begin position="1"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01547"/>
        </source>
    </evidence>
    <sequence length="230" mass="25029" checksum="00B9F4CB8F75D4A7" modified="2005-10-11" version="1">MAKDTTGRLHVTVKTGGKRKLSSKLWLERQLNDPYVAQAKRDGWRSRASFKLIEMDDKHRFLKPGMTVVDLGAAPGGWSQVAAKRVGAAEGKGRVIAIDLLEMPEIVGVTFARLDFLDDSAPDKLLAMMDGAADVVLSDMAANTTGHRKTDQLRIVGLVESAAAFTSDVLRPGGTFIAKVFQSGADAGLLVQLKRDFQTVRHVKPAASRQDSSERYVMATGFRGGRRDKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQQ2</accession>
    <name>Q3SQQ2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2134</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315360.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2134"/>
    <dbReference type="EnsemblBacteria" id="ABA05389">
        <property type="protein sequence ID" value="ABA05389"/>
        <property type="gene ID" value="Nwi_2134"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2134"/>
    <dbReference type="eggNOG" id="COG5489">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109334_1_0_5"/>
    <dbReference type="OMA" id="SNEGREY"/>
    <dbReference type="OrthoDB" id="1730357at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007948">
        <property type="entry name" value="DUF736"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05284">
        <property type="entry name" value="DUF736"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="107" mass="11786" checksum="247D1239E84FD1A7" modified="2005-10-11" version="1">MATIGTFKKSGNEFVGQIVTLSVQAKNVRIVPEANRSGENAPSHRVFAGRVEIGAAWAKRSNEGRDYLGLKLDDPSFTAPIYANLFDDEDGDGYSLIWSRPNRRNGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQT0</accession>
    <name>Q3SQT0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Integrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2106</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2106"/>
    <dbReference type="EnsemblBacteria" id="ABA05361">
        <property type="protein sequence ID" value="ABA05361"/>
        <property type="gene ID" value="Nwi_2106"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2106"/>
    <dbReference type="eggNOG" id="COG0582">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027562_0_4_5"/>
    <dbReference type="OMA" id="DHAEDIW"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038488">
        <property type="entry name" value="Integrase_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025166">
        <property type="entry name" value="Integrase_DNA_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13356">
        <property type="entry name" value="Arm-DNA-bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyr recombinase" evidence="3">
        <location>
            <begin position="199"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="272" mass="30772" checksum="652DAE230FDDBC9F" modified="2005-10-11" version="1">MLTDTELKSLKPRAKNYKVTDRDGMYVLVIPSGAKTFRMDYRLNGRRETLTIGRYGPGGLSLAEARDRCLAAKKDVAEGRSPAQEKQRAKRRVALGETFGAVGQRWIKSAPMADSTRAMRKAIFDRDILPVWKNRLVTEITPDDLRAQCIKVKDRGAPATAIHVRDIVKQIYAYAILNGEKVLNPAEEVGPASIATFAPKDRSLSPTEIRIMFEQLRHVPTLPTIRLGLRLILLTMVRKSELLDAVWSEVDFENAVWSIPKERMKRRKPHNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQT1</accession>
    <name>Q3SQT1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Integrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2105</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2105"/>
    <dbReference type="EnsemblBacteria" id="ABA05360">
        <property type="protein sequence ID" value="ABA05360"/>
        <property type="gene ID" value="Nwi_2105"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2105"/>
    <dbReference type="eggNOG" id="COG0582">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027562_40_3_5"/>
    <dbReference type="OMA" id="NHASYVE"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyr recombinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="149" mass="16881" checksum="4BEC12F281213B8D" modified="2005-10-11" version="1">MSNLSRQSLDIMIALKTCAGNSRYLLPSRYDADAPMSRATFNRITTAVADRAKKLGLALEPFTVHDLRRTGSTLLNEIGFNSDWIEKCLAHEDGRSSRGVYNKAEYEHQRRHMLQEWADLVDAWEQDRNHTPVLYPPAMQFPGVMAATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR30</accession>
    <name>Q3SR30_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2002</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315247.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2002"/>
    <dbReference type="EnsemblBacteria" id="ABA05261">
        <property type="protein sequence ID" value="ABA05261"/>
        <property type="gene ID" value="Nwi_2002"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2002"/>
    <dbReference type="eggNOG" id="ENOG5030YCE">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2749790_0_0_5"/>
    <dbReference type="OMA" id="MNYLDAM"/>
    <dbReference type="OrthoDB" id="2036933at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="68" mass="7557" checksum="463CCB5DA692536C" modified="2005-10-11" version="1">MCLLCGDEKAYRAYMDYLDAMERQGRTADPDEAANAVLARLHAVDTASADDPRNDRTLSPFFCSAVDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR41</accession>
    <name>Q3SR41_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1991</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315237.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1991"/>
    <dbReference type="EnsemblBacteria" id="ABA05250">
        <property type="protein sequence ID" value="ABA05250"/>
        <property type="gene ID" value="Nwi_1991"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1991"/>
    <dbReference type="eggNOG" id="ENOG5032VW1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1093891_0_0_5"/>
    <dbReference type="OMA" id="VWLLWSW"/>
    <dbReference type="OrthoDB" id="2063185at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="226"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="252" mass="27294" checksum="DCC7D901B93944A7" modified="2005-10-11" version="1">MIALLLALILLPLFPLSMLFNAGLQVLPVWLRVPALVAFPAAGAWLLNQAAPPPEQYVLPLQLLAAFTALLYAWRLLSVQEVFIWARLQATSVWPLVWLAWLHGLHGKTLVFVALAMTIPAAVLMILGSSLSSRFGGAYLGLRGRIGPAFPRLTLAWVVTLLAALAAPPFPGFFAMLAVLQQIPVGLTVVVLAVWLLWSWAAALLWQHGLFGREWPRPAGTVDLSYGAGLGLMLTAVAAVLASIGGWSWWMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR53</accession>
    <name>Q3SR53_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">4a-hydroxytetrahydrobiopterin dehydratase</fullName>
            <ecNumber evidence="3">4.2.1.96</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">4-alpha-hydroxy-tetrahydropterin dehydratase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Pterin carbinolamine dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_1979</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin = (6R)-L-erythro-6,7-dihydrobiopterin + H2O</text>
            <dbReference type="Rhea" id="RHEA:11920"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15642"/>
            <dbReference type="ChEBI" id="CHEBI:43120"/>
            <dbReference type="EC" id="4.2.1.96"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pterin-4-alpha-carbinolamine dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.96" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315225.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1979"/>
    <dbReference type="EnsemblBacteria" id="ABA05238">
        <property type="protein sequence ID" value="ABA05238"/>
        <property type="gene ID" value="Nwi_1979"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1979"/>
    <dbReference type="eggNOG" id="COG2154">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_179139_0_0_5"/>
    <dbReference type="OMA" id="GWERRDK"/>
    <dbReference type="OrthoDB" id="1931240at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008124">
        <property type="term" value="F:4-alpha-hydroxytetrahydrobiopterin dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036428">
        <property type="entry name" value="PCD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001533">
        <property type="entry name" value="Pterin_deHydtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01329">
        <property type="entry name" value="Pterin_4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55248">
        <property type="entry name" value="SSF55248"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="88" mass="9953" checksum="19AD0741FA309908" modified="2005-10-11" version="1">MNDDFQGWERRDKPPTLFRRFAFAQYAQTRAFLDALSALSEEIQRHPQNINFGTTYVNITIGAVDDTALSAEDEQFAGRIAALAERGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR74</accession>
    <name>Q3SR74_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, XRE family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1957</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain fatty acyl-CoA assimilation regulator (ScfR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1957"/>
    <dbReference type="EnsemblBacteria" id="ABA05217">
        <property type="protein sequence ID" value="ABA05217"/>
        <property type="gene ID" value="Nwi_1957"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1957"/>
    <dbReference type="eggNOG" id="COG2856">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053651_0_1_5"/>
    <dbReference type="OrthoDB" id="1840229at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010359">
        <property type="entry name" value="IrrE_HExxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06114">
        <property type="entry name" value="Peptidase_M78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="2">
        <location>
            <begin position="11"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="410" mass="44928" checksum="1BB3146D93CEE08B" modified="2005-10-11" version="1">MIDAAQLGDRLRAARERRGLSQQAVAEALGLPRTAVTNMESGTRSVSTLELTRLADLYGQQATFFLSMHEEAEDLSIVLHRALPEMAGSPHLDGEVRRILDLYQEGAGLRALLGRTVELSIPDYAAKLFSVGDAIRQGEDAALEERRRLDLGAAPIVNMAALISEQGIWTAATDLPDGMSGLFINHAAIGPSILVNAGHWPVRRRFSYAHEYAHALFDRVVTVTTTRRENASELVEKRANAFAAAFLMPPDGVADALRQLDKGQPSRLTQIIFDVAGNTGLEAEIRARPGSQAITYQDAATLARHFGVSYEAMVWRLKSLNHIGGPDTDALMARKDTARRAIRLLGFLDLLDEGDVPPPPEPELRSQLMRLSVEAYRQEEISRGRLLELARKLAIDGSELIELADATRAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR97</accession>
    <name>Q3SR97_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transposase, IS4 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0435</name>
        <name evidence="4" type="ordered locus">Nwi_1934</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="4">Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03702">
        <property type="protein sequence ID" value="ABA03702"/>
        <property type="gene ID" value="Nwi_0435"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05194">
        <property type="protein sequence ID" value="ABA05194"/>
        <property type="gene ID" value="Nwi_1934"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0435"/>
    <dbReference type="KEGG" id="nwi:Nwi_1934"/>
    <dbReference type="eggNOG" id="COG3293">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055261_9_1_5"/>
    <dbReference type="OMA" id="KYKVRQF"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE_Tnp_1" evidence="2">
        <location>
            <begin position="33"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="176" mass="19358" checksum="228F32F8FF928F61" modified="2005-10-11" version="1">MGTGVLHPDSRSRQPVSDARLNHRSSPSAGGYRKRGAKDQALGRSRGGLTTKVHMLADALGRPLRFIVTAGQVGDITQAPALLEAQAGDAVMADKAYDSNALRALIAGMGAEAVIPSNRTRKIIIPHDAGLYKHRNRIERCFNRLKHFRRFATRYDRRTVHFTGFVHLAAALIWLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRE8</accession>
    <name>Q3SRE8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">NADH dehydrogenase subunit E</fullName>
            <ecNumber evidence="8">1.6.5.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_1883</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="6">Binds 1 [2Fe-2S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 24 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.3" evidence="8"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315139.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1883"/>
    <dbReference type="EnsemblBacteria" id="ABA05143">
        <property type="protein sequence ID" value="ABA05143"/>
        <property type="gene ID" value="Nwi_1883"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1883"/>
    <dbReference type="eggNOG" id="COG1905">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054362_1_2_5"/>
    <dbReference type="OMA" id="VGKFHVQ"/>
    <dbReference type="OrthoDB" id="1206880at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03064">
        <property type="entry name" value="TRX_Fd_NuoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002023">
        <property type="entry name" value="NuoE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042128">
        <property type="entry name" value="NuoE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041921">
        <property type="entry name" value="NuoE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000216">
        <property type="entry name" value="NADH_DH_24kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01958">
        <property type="entry name" value="nuoE_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01099">
        <property type="entry name" value="COMPLEX1_24K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="172"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="224"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000216-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA05143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="250" mass="27441" checksum="57A1F8D9E78FA852" modified="2005-10-11" version="1">MSVRRLAPKEQQPESFAFSEENLAWAKKQIAQYPAGRQASAAIAILWRAQEQNGGWISEAAIRTVADMLDMPYIRMLEIATFYTMFQLQPVGRKAHIQVCGTTPCRLRGAEDILAVCKSRIHHEPFHLSKDGDFSWEEVECLGSCVNAPMVLIWKDTYEDLTKENFGKVLDGFASGQPPKPGPQIDRQFSAPVGGPTTLNTAAKGDGGRRSAAQPALTDADAKKPTDTASRRESPMPKPPADDATRKGKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRG5</accession>
    <name>Q3SRG5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nuclear export factor GLE1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1866</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1866"/>
    <dbReference type="EnsemblBacteria" id="ABA05126">
        <property type="protein sequence ID" value="ABA05126"/>
        <property type="gene ID" value="Nwi_1866"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1866"/>
    <dbReference type="eggNOG" id="COG4549">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087540_1_1_5"/>
    <dbReference type="OMA" id="RWIEIPA"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd08545">
        <property type="entry name" value="YcnI_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012533">
        <property type="entry name" value="YcnI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038507">
        <property type="entry name" value="YcnI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07987">
        <property type="entry name" value="DUF1775"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229222" evidence="2">
        <location>
            <begin position="28"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1775" evidence="3">
        <location>
            <begin position="28"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="176" mass="18914" checksum="4A9018D5EE3F56FB" modified="2005-10-11" version="1">MMRVSGSWIGSAIAAAAVVASWEAALAHVTVQPADAPADSYARLTFTVPHGCNGSATTVLRIRLPEGILSVKPQMKPGWNVDIKSRKLEAPVQGPHGKTVTDVVEEVIWRGGPLPDNLYDTFGLVVRLPDKAGQSLYFPAEQECEQGSQRWTEIPQAGQADKPRAPAPVVRLKAKP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-03-24" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRI8</accession>
    <name>SYQ_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glutaminyl-tRNA synthetase</fullName>
            <shortName evidence="1">GlnRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">glnS</name>
        <name type="ordered locus">Nwi_1843</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln)</text>
            <dbReference type="Rhea" id="RHEA:20121"/>
            <dbReference type="Rhea" id="RHEA-COMP:9662"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.18"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.18" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315099.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRI8"/>
    <dbReference type="SMR" id="Q3SRI8"/>
    <dbReference type="STRING" id="323098.Nwi_1843"/>
    <dbReference type="EnsemblBacteria" id="ABA05103">
        <property type="protein sequence ID" value="ABA05103"/>
        <property type="gene ID" value="Nwi_1843"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1843"/>
    <dbReference type="eggNOG" id="COG0008">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001882_2_3_5"/>
    <dbReference type="OMA" id="INNFCAQ"/>
    <dbReference type="OrthoDB" id="142899at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004819">
        <property type="term" value="F:glutamine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006425">
        <property type="term" value="P:glutaminyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00126">
        <property type="entry name" value="Gln_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004514">
        <property type="entry name" value="Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022861">
        <property type="entry name" value="Gln_tRNA_ligase_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020059">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_codon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020061">
        <property type="entry name" value="Glu_tRNA_lig_a-bdl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_L25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_L25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03950">
        <property type="entry name" value="tRNA-synt_1c_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="SSF50715"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00440">
        <property type="entry name" value="glnS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000095495" description="Glutamine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="1">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="1">
        <location>
            <begin position="270"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="37"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="43"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="263"/>
            <end position="264"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="271"/>
            <end position="273"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00126"/>
        </source>
    </evidence>
    <sequence length="559" mass="63415" checksum="DF09B74C14F9C3DA" modified="2005-10-11" version="1">MTDESTAEAGRDFIRDIVAADLASGRHKSIVTRFPPEPNGYLHLGHAKSICLNFGIAEDFGGRCHLRFDDTNPAKEEQEFIDAIQRDVRWLGFDWGEHLYYASDYFEQLYAWAQHLIREGKAYVDDQTQEEIRLARGTLTEPGRNSPFRDRPADENLDLFARMRAGEFPNGARVLRAKIDMAAGNINLRDPVLYRILHATHPRTGNVWSIYPSYDFAHGQSDSIEHITHSVCTLEFEDHRPLYDWFLDNLPVPSRPRQYEFARLNVTHTLLSKRVLTELVRGGHVAGWDDPRMPTLAGLQRRGVPAEALREFVKRIGVAKANSTVDVGMLDFAIREALNRSASRRMAVLRPLKVVIENYPEGQSEELEAVNHPDDPSQGTRRIRFGREIYIEQEDFMENPPKKFFRLSPGREVRLRYAYFITCREVVKNPAGDVLELRCTYDPATRGGNAPDGRKVKATMHWVNTADAVPAEVRLYGHLFETEQPDAANFASELNPQSVQILSGCMVEPALARDDAGAAVQFERQGYFYRDRDSAPGRLVFNRTVGLRDTWAKVAAAGS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRM3</accession>
    <name>SYR_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Arginine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Arginyl-tRNA synthetase</fullName>
            <shortName evidence="1">ArgRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argS</name>
        <name type="ordered locus">Nwi_1807</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg)</text>
            <dbReference type="Rhea" id="RHEA:20301"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.19"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.19" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315064.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRM3"/>
    <dbReference type="SMR" id="Q3SRM3"/>
    <dbReference type="STRING" id="323098.Nwi_1807"/>
    <dbReference type="EnsemblBacteria" id="ABA05068">
        <property type="protein sequence ID" value="ABA05068"/>
        <property type="gene ID" value="Nwi_1807"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1807"/>
    <dbReference type="eggNOG" id="COG0018">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006406_0_1_5"/>
    <dbReference type="OMA" id="NKPLHLG"/>
    <dbReference type="OrthoDB" id="1146366at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00671">
        <property type="entry name" value="ArgRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00123">
        <property type="entry name" value="Arg_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001278">
        <property type="entry name" value="Arg-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005148">
        <property type="entry name" value="Arg-tRNA-synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036695">
        <property type="entry name" value="Arg-tRNA-synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035684">
        <property type="entry name" value="ArgRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008909">
        <property type="entry name" value="DALR_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956">
        <property type="entry name" value="PTHR11956"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03485">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05746">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00750">
        <property type="entry name" value="tRNA-synt_1d"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01038">
        <property type="entry name" value="TRNASYNTHARG"/>
    </dbReference>
    <dbReference type="SMART" id="SM01016">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00836">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55190">
        <property type="entry name" value="SSF55190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00456">
        <property type="entry name" value="argS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000242055" description="Arginine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="138"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00123"/>
        </source>
    </evidence>
    <sequence length="597" mass="65428" checksum="A6DF8E91710B0424" modified="2005-10-11" version="1">MTDSSPSQHLFADVLARVHRACAALAEEGALPSGLDLARIVVEPPRDASHGDMATNAAMVLAKDAKAKPRDLAERIVTKLRADDLIESADIAGPGFINLTLKLPVWADVLRDVLRDGEGYGRSAIGAGEKVNVEYVSANPTGPMHVGHCRGAVFGDALASLLSFAGYDVTREYYINDAGSQVDVLARSAFLRYREALGEDIGEIPDGLYPGDYLKPVGQALAAGHGDSLVKAPEAEWLGLVRAKAIGMMMEMIRGDLAALNIRHDVFFSERSLIDGSADRVAETIGFLREKGDVYEGRLPPPKGAPVEDYEDREQTLFRATAYGDDVDRPLKKSDGGYTYFASDIAYHKTKFDRGFHNMVDVWGADHGGYIKRVQAAIKAVTSGKGTLDVKIVQLVKLLRNGEPVKMSKRAGDFVTLREVVEEVGSDAVRFMMLFRKNDAVLDFDLARVIEQSKDNPVFYVQYGHARGFSIFRNAREVFPDLPEADAERRQFLGTADLERLSDPAELGLLRKLALYPRTVEAAAAAHEPHRIAFYLYDLASEFHALWTKGRDLPHLRFIINNDAQITRARLALVQGVVAVLASGLTVLGVHAPTEMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRX1</accession>
    <name>Q3SRX1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, XRE family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1709</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1709"/>
    <dbReference type="EnsemblBacteria" id="ABA04970">
        <property type="protein sequence ID" value="ABA04970"/>
        <property type="gene ID" value="Nwi_1709"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1709"/>
    <dbReference type="eggNOG" id="COG2944">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144725_1_0_5"/>
    <dbReference type="OMA" id="WEQGKRM"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="69"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="130" mass="14452" checksum="4655A6F4F103AD83" modified="2005-10-11" version="1">MPRPRAHSLRLNVHHRRSLESEVSMQRLRLKADGRIVEIRDGKEFPLSPSMHVAGAASDDAGPSSVPEVRSLRRRARLTQVEFAARLGVPVETIRNWEQGKRIPRGPARALLAVVAHAPETVFAALDGRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS21</accession>
    <name>Q3SS21_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">HesB/YadR/YfhF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1659</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HesB/IscA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314919.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1659"/>
    <dbReference type="EnsemblBacteria" id="ABA04920">
        <property type="protein sequence ID" value="ABA04920"/>
        <property type="gene ID" value="Nwi_1659"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1659"/>
    <dbReference type="eggNOG" id="COG0316">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069054_4_2_5"/>
    <dbReference type="OMA" id="GYQYGMA"/>
    <dbReference type="OrthoDB" id="1891602at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.300.12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000361">
        <property type="entry name" value="FeS_biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016092">
        <property type="entry name" value="FeS_cluster_insertion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035903">
        <property type="entry name" value="HesB-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01521">
        <property type="entry name" value="Fe-S_biosyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89360">
        <property type="entry name" value="SSF89360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00049">
        <property type="entry name" value="TIGR00049"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe-S_biosyn" evidence="2">
        <location>
            <begin position="22"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="134" mass="14450" checksum="F87FB6D498A5479E" modified="2005-10-11" version="1">MTDMTPGTPATKPKPRPRPQVMRLTDAAATRVKELAARADSEIVGLRVGIKNGGCAGQSYTVEYAHDIRPTDEVVEDKGVKILVDPKAVLYLLGTEMDYKAEKMQAQFVFNNPNQVSACGCGESVQLTPAKIDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS23</accession>
    <name>Q3SS23_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">DEAD/DEAH box helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_1657</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314917.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1657"/>
    <dbReference type="EnsemblBacteria" id="ABA04918">
        <property type="protein sequence ID" value="ABA04918"/>
        <property type="gene ID" value="Nwi_1657"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1657"/>
    <dbReference type="eggNOG" id="COG0513">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_28_1_5"/>
    <dbReference type="OMA" id="EIKQESM"/>
    <dbReference type="OrthoDB" id="643629at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6 11" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Q_MOTIF" evidence="10">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="32"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="234"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="391"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="411"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA04918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="483" mass="52015" checksum="A87F615F6ADDBCF8" modified="2005-10-11" version="1">MSFSHLGLSDKVLAAVAAAGYTSPTPIQDQAIPHVLARRDVLGIAQTGTGKTAAFVLPMLTLLEKGRARARMPRTLILEPTRELAAQVKEQFDKYGAGQKLNVALLIGGVSFGDQDLKLTRGVDVLIATPGRLLDHTERGGLLLTGVELLVIDEADRMLDMGFIPDIERICKLVPFTRQTLFFTATMPNEIRRVTDTFLHNPEKIEVSRPATTAATVAQSQVSCSREPHEKRELLRRLLREAKELKNAIIFCNRKRDVAIVCKSLQKHGFSAGALHGDMDQSARTASLDQFRKGELPLLVASDVAARGLDIPEVSHVLNFDVPYHPDDYVHRIGRTGRAGRTGTAITIVCPADFKSLAAIEKLTGQAIPQADLPGHDASANEAVVEAGAPAAAAAGRSSRSRSAPDKGGESYARGGKRESRKPRRSGGDHQRHEAPAVTAAASKAAPPPSIGRTLAPRREAVSEPGDHSHLPAFLLRPIRARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS54</accession>
    <name>Q3SS54_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1483</name>
        <name evidence="3" type="ordered locus">Nwi_1626</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="3">Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314751.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1483"/>
    <dbReference type="MEROPS" id="S78.001"/>
    <dbReference type="EnsemblBacteria" id="ABA04744">
        <property type="protein sequence ID" value="ABA04744"/>
        <property type="gene ID" value="Nwi_1483"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04887">
        <property type="protein sequence ID" value="ABA04887"/>
        <property type="gene ID" value="Nwi_1626"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1483"/>
    <dbReference type="KEGG" id="nwi:Nwi_1626"/>
    <dbReference type="eggNOG" id="COG3740">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073043_2_1_5"/>
    <dbReference type="OMA" id="VAWKFAT"/>
    <dbReference type="OrthoDB" id="1734449at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006433">
        <property type="entry name" value="Prohead_protease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04586">
        <property type="entry name" value="Peptidase_S78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01543">
        <property type="entry name" value="proheadase_HK97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-1188">Viral release from host cell</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="165" mass="17787" checksum="78DF7D14C4327209" modified="2005-10-11" version="1">MDRLEIKASLNVSDTGEIVGNAWPFAEPDRVGDIITKGAFGEISPDLGMLYQHNPADLIGTWTEVKATDEGLMVKGQLHLDQPRARSVRSLVLKGLVSDLSIGFRTKSSRKEGRNRIIDALDLAEISLVRSGSHPRARITSAKSFDEAAAIAAVINRAAAALALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSB8</accession>
    <name>Q3SSB8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1562</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314829.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1562"/>
    <dbReference type="EnsemblBacteria" id="ABA04823">
        <property type="protein sequence ID" value="ABA04823"/>
        <property type="gene ID" value="Nwi_1562"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1562"/>
    <dbReference type="HOGENOM" id="CLU_2650798_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="76" mass="8096" checksum="76A96AFC552CCE84" modified="2005-10-11" version="1">MRNRPSPAFIAKVLDALPADGAPISSRGVFLAIGEISTPAYVRIVLRQLAIGGRIIATGPSGQRVYRRLPAQQEVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSB9</accession>
    <name>Q3SSB9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1561</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314828.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1561"/>
    <dbReference type="EnsemblBacteria" id="ABA04822">
        <property type="protein sequence ID" value="ABA04822"/>
        <property type="gene ID" value="Nwi_1561"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1561"/>
    <dbReference type="HOGENOM" id="CLU_2618442_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="81" mass="8623" checksum="9BACCF6A3DD90A32" modified="2005-10-11" version="1">MAPSDTAQSNVITLPRLEAGDLIDRMAADLIHFEATGTEGDAIRSLMATRRYGYGEIATLIDDALAAARQEIVAAEMARAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSD1</accession>
    <name>Q3SSD1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcription antitermination protein nusG</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_1549</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314816.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1549"/>
    <dbReference type="EnsemblBacteria" id="ABA04810">
        <property type="protein sequence ID" value="ABA04810"/>
        <property type="gene ID" value="Nwi_1549"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1549"/>
    <dbReference type="eggNOG" id="COG0250">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067287_1_0_5"/>
    <dbReference type="OMA" id="RNISAYC"/>
    <dbReference type="OrthoDB" id="2060826at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006645">
        <property type="entry name" value="NGN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036735">
        <property type="entry name" value="NGN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043425">
        <property type="entry name" value="NusG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30265">
        <property type="entry name" value="PTHR30265"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02357">
        <property type="entry name" value="NusG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82679">
        <property type="entry name" value="SSF82679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0889">Transcription antitermination</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="KOW" evidence="5">
        <location>
            <begin position="158"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="203"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="220"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA04810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="234" mass="26448" checksum="020FCE954F98B9B6" modified="2005-10-11" version="1">MIGELQRLEIGQFVGTVDLDAVRGPASVPMQPKYWFILRTHPGCERKVMREFERRNISAYCPLIAKQQRVVRRVHGSSWTYEIKRLVQVPLFPQLVFVPDFERVPADIGGVSGWLRFGGWRARIPSEREATSDRVACMADIHALVAIANTPQSKRAARFEIGALVRIVDGPFRDFSGRIERLDSKGRLKVAIEIFGRLSPTEMSEAQIEPSPGPTDTCTVREGRKPRRGSFSCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSE9</accession>
    <name>Q3SSE9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Lipid A biosynthesis</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1531</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314798.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1531"/>
    <dbReference type="EnsemblBacteria" id="ABA04792">
        <property type="protein sequence ID" value="ABA04792"/>
        <property type="gene ID" value="Nwi_1531"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1531"/>
    <dbReference type="eggNOG" id="COG3952">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161206_1_1_5"/>
    <dbReference type="OMA" id="RFVVQWI"/>
    <dbReference type="OrthoDB" id="1964542at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008915">
        <property type="term" value="F:lipid-A-disaccharide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009245">
        <property type="term" value="P:lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011499">
        <property type="entry name" value="Lipid_A_biosynth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014546">
        <property type="entry name" value="UCP028440_lipidA_biosyn"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07578">
        <property type="entry name" value="LAB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028440">
        <property type="entry name" value="UCP_LAB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01259">
        <property type="entry name" value="LAB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="LAB_N" evidence="2">
        <location>
            <begin position="26"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="107" mass="12272" checksum="F23C6902154DE0C4" modified="2005-10-11" version="1">MTDMVGMLTNYFYDVFVANFDGWVILGFVAQGFFTMRFVVQWIASERARKSVIPVAFWFFSIGGGVLLLVYALYRRDPVFIAGQALGLIVYFRNLYFIILNGRQSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSM2</accession>
    <name>Q3SSM2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alkylhydroperoxidase AhpD core</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1458</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314726.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1458"/>
    <dbReference type="PeroxiBase" id="4771">
        <property type="entry name" value="NwCMD"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04719">
        <property type="protein sequence ID" value="ABA04719"/>
        <property type="gene ID" value="Nwi_1458"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1458"/>
    <dbReference type="eggNOG" id="COG0599">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137228_2_1_5"/>
    <dbReference type="OMA" id="CIAVHSQ"/>
    <dbReference type="OrthoDB" id="1925754at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004675">
        <property type="entry name" value="AhpD_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="SSF69118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00778">
        <property type="entry name" value="ahpD_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMD" evidence="1">
        <location>
            <begin position="27"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="114" mass="11810" checksum="8380A4DAFB3FD736" modified="2005-10-11" version="1">MLDWANFRNKLSSRVGELRKLSPDTVDAYRMAGGAGAKTGHLDGKTRELISLAVAVTTRCDGCIAVHAAKAVQEGATREEIAEALGVAVAMNAGAAVVFSARVLDAVDAVEGAR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSW9</accession>
    <name>EFG_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Elongation factor G</fullName>
            <shortName evidence="1">EF-G</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fusA</name>
        <name type="ordered locus">Nwi_1361</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314639.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSW9"/>
    <dbReference type="SMR" id="Q3SSW9"/>
    <dbReference type="STRING" id="323098.Nwi_1361"/>
    <dbReference type="EnsemblBacteria" id="ABA04622">
        <property type="protein sequence ID" value="ABA04622"/>
        <property type="gene ID" value="Nwi_1361"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1361"/>
    <dbReference type="eggNOG" id="COG0480">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002794_4_1_5"/>
    <dbReference type="OMA" id="AATTCHW"/>
    <dbReference type="OrthoDB" id="88967at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16262">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01434">
        <property type="entry name" value="EFG_mtEFG1_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03713">
        <property type="entry name" value="EFG_mtEFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00054_B">
        <property type="entry name" value="EF_G_EF_2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009022">
        <property type="entry name" value="EFG_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035649">
        <property type="entry name" value="EFG_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004540">
        <property type="entry name" value="Transl_elong_EFG/EF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00484">
        <property type="entry name" value="EF-G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0251">Elongation factor</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000225221" description="Elongation factor G">
        <location>
            <begin position="1"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="domain" description="tr-type G">
        <location>
            <begin position="8"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="17"/>
            <end position="24"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="81"/>
            <end position="85"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="135"/>
            <end position="138"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00054"/>
        </source>
    </evidence>
    <sequence length="690" mass="75753" checksum="CE82DA7CCC15D7C6" modified="2005-10-11" version="1">MPRQHAIEDYRNFGIMAHIDAGKTTTTERILYYTGKSHKIGEVHEGAATMDWMTQEQERGITITSAATTAFWDGKRLNIIDTPGHVDFTIEVERSLRVLDGAVCVLDSNQGVEPQTETVWRQGDKYKVPRIVFCNKMDKTGADFYKCLADIVDRLGARPVALQLPIGSESNFKGMVDLVRMKALVWNNEALGAMYDIVDIPADLADKAKEYREKLVEAAVELDDDAMAAYLDGAEPDEATLKKLIRKAVLTGAFYPVLCGTAFKNKGVQPLLDAVVAYLPSPLDVPAIKGVDDKGNEVVRHADDKEPMSLLAFKIMDDPFVGTITFCRIYSGILQSGTGVVNSTREKKERIGRMLLMHANNREDIKEAYAGDIVALAGLKEARTGDTLCDPAHQVILEKMEFPDPVIEIAIEPKSKADQEKLGIALAKLAAEDPSFRVSTDQESGQTILKGMGELHLDIKVDILKRTYKVDANIGAPQVAFRERVTKRVEHSYTHKKQTGGTGQFAAVTLIVEPSEPGKGYEFESKIVGGAVPKEYIPGVEKGIESVLSSGVVAGFPVVDVKVQLIDGKFHDVDSSALAFEIATRACFREALQKGKSVLLEPIMKVEVVTPEDYTGSVIGDLNSRRGQIQGQDMRGNANVINAMVPLMNMFGYVNNLRSMSQGRATFTMQFDHYAEAPANVSAEVQKKFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST35</accession>
    <name>Q3ST35_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ</fullName>
            <ecNumber evidence="5">2.1.1.200</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">tRNA (cytidine(32)/uridine(32)-2'-O)-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">tRNA Cm32/Um32 methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">trmJ</name>
        <name evidence="8" type="ordered locus">Nwi_1295</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>S-adenosyl-L-methionine + uridine(32) in tRNA = 2'-O-methyluridine(32) in tRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42936"/>
            <dbReference type="Rhea" id="RHEA-COMP:10107"/>
            <dbReference type="Rhea" id="RHEA-COMP:10290"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74478"/>
            <dbReference type="EC" id="2.1.1.200"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>cytidine(32) in tRNA + S-adenosyl-L-methionine = 2'-O-methylcytidine(32) in tRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42932"/>
            <dbReference type="Rhea" id="RHEA-COMP:10288"/>
            <dbReference type="Rhea" id="RHEA-COMP:10289"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.200"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.200" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314579.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1295"/>
    <dbReference type="EnsemblBacteria" id="ABA04556">
        <property type="protein sequence ID" value="ABA04556"/>
        <property type="gene ID" value="Nwi_1295"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1295"/>
    <dbReference type="eggNOG" id="COG0565">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2852">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041885_0_0_5"/>
    <dbReference type="OMA" id="FAMPERS"/>
    <dbReference type="OrthoDB" id="2013215at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007569">
        <property type="entry name" value="DUF559"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004384">
        <property type="entry name" value="RNA_MeTrfase_TrmJ/LasT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42786">
        <property type="entry name" value="PTHR42786"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04480">
        <property type="entry name" value="DUF559"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00050">
        <property type="entry name" value="rRNA_methyl_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SpoU_methylase" evidence="6">
        <location>
            <begin position="17"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="DUF559" evidence="7">
        <location>
            <begin position="291"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA04556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="403" mass="43761" checksum="41F9A0C08FD1EF1C" modified="2005-10-11" version="1">MSGTDRTKTGIDVVGPTVILVEPQLGENIGMAARAMGNFALTRLRIVNPRDGWPNIAAQRAAAGADHILSRVELFDTVAEAVADCTLLFATTARAHDQAKPVVAPEAAAVEATAQIAAGGHVGILFGRERAGLLNEEVAMADRIITYPVNPGFASLNLAQAVLLIGYEWFKLVTRGELPFAMPERSERASQHQMQAFFDNLVAELDKVEFLRPPEKRDTMLVNLRNIFTRMEPTKQDMHTLHGVVMAIAEGRKGPAKGGVLDGAQATRLRALLAEQGPGGASADSSSTVRGLARLLRRNPTDAERILWQALTRDRRFAGQFKRQTPVGRHIPDFVSFVQRVAIELVNADESDTIARDRAARSAWLAERGYRVIEMEAAAVEADLAHVLDGLAGRMESGKDPKP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST50</accession>
    <name>RECA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein RecA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Recombinase A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">recA</name>
        <name type="ordered locus">Nwi_1280</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RecA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314569.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ST50"/>
    <dbReference type="SMR" id="Q3ST50"/>
    <dbReference type="STRING" id="323098.Nwi_1280"/>
    <dbReference type="EnsemblBacteria" id="ABA04541">
        <property type="protein sequence ID" value="ABA04541"/>
        <property type="gene ID" value="Nwi_1280"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1280"/>
    <dbReference type="eggNOG" id="COG0468">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040469_1_2_5"/>
    <dbReference type="OMA" id="DYGEQAL"/>
    <dbReference type="OrthoDB" id="1080436at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00983">
        <property type="entry name" value="recA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00268">
        <property type="entry name" value="RecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013765">
        <property type="entry name" value="DNA_recomb/repair_RecA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020584">
        <property type="entry name" value="DNA_recomb/repair_RecA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023400">
        <property type="entry name" value="RecA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020587">
        <property type="entry name" value="RecA_monomer-monomer_interface"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45900">
        <property type="entry name" value="PTHR45900"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00154">
        <property type="entry name" value="RecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00142">
        <property type="entry name" value="RECA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54752">
        <property type="entry name" value="SSF54752"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02012">
        <property type="entry name" value="tigrfam_recA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00321">
        <property type="entry name" value="RECA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50163">
        <property type="entry name" value="RECA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0742">SOS response</keyword>
    <feature type="chain" id="PRO_1000047955" description="Protein RecA">
        <location>
            <begin position="1"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="77"/>
            <end position="84"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00268"/>
        </source>
    </evidence>
    <sequence length="362" mass="38773" checksum="BC9A5C22B596F727" modified="2005-10-11" version="1">MSQAALRIVEGSSMDKSKALSAALSQIERQFGKGSVMKLGKNDKSMDVEVISSGSLGLDIALGVGGLPKGRIVEVYGPESSGKTTLALHAVAEAQKKGGICAFIDAEHALDPVYARKLGVNVDDLLISQPDHGEQALEIADTLVRSGAVDVLIIDSVAALVPRAELEGEMGDALPGLQARLMSQALRKLTASINKSNTMVIFINQIRMKIGVMYGSPETTTGGNALKFYASVRLDIRRIGAIKERDEVVGNQTRVKVVKNKLAPPFKQVEFDIMYGEGISKMGEILDLGVKAGIVEKSGAWFSYDSQRMGQGRENAKAFLRANPDITAKVEMAIRQNSGLIAEQILAGSPERETDDDETAED</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STF1</accession>
    <name>Q3STF1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1178</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314469.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1178"/>
    <dbReference type="EnsemblBacteria" id="ABA04440">
        <property type="protein sequence ID" value="ABA04440"/>
        <property type="gene ID" value="Nwi_1178"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1178"/>
    <dbReference type="eggNOG" id="COG5448">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114534_0_0_5"/>
    <dbReference type="OMA" id="DQRIGTG"/>
    <dbReference type="OrthoDB" id="2005986at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011740">
        <property type="entry name" value="DUF2460"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09343">
        <property type="entry name" value="DUF2460"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02217">
        <property type="entry name" value="chp_TIGR02217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="213" mass="22924" checksum="CEC05A7DBC193EC3" modified="2005-10-11" version="1">MTSFHEVLFPLDVALKSAGGPERRTDIVSFGSGREARNARWAQSRRRFDAGYGVKTLEALQAVVAFFEERRGRLYGFRWRDRLDSCSAATGSVISPLDQVIGIGDGTASTFQLIKTYGDAFAPYARAIGKPVNDSVRVAVAESEVSAGSAFTCDATTGVVTFLPEHTPPPGAVVTAGFKFDVPVRFDTDYLEVDLSTFAAGAIPKIPLVEILP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STG3</accession>
    <name>Q3STG3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1166</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314457.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04428">
        <property type="protein sequence ID" value="ABA04428"/>
        <property type="gene ID" value="Nwi_1166"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1166"/>
    <dbReference type="HOGENOM" id="CLU_161989_0_0_5"/>
    <dbReference type="OrthoDB" id="2224645at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="108" mass="11371" checksum="70BB58EBD5FFCC20" modified="2005-10-11" version="1">MVPGNGSSTSRRTMRFLIFVWITLAVAATASAWFAMRPSRRGAAWIGIAACVVLLATFASSFLMTSVHGDKTMLSIALYLLNWTMAAGGAAICIGAIAGLTLGLAFKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STL1</accession>
    <name>Q3STL1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1118</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1118"/>
    <dbReference type="EnsemblBacteria" id="ABA04380">
        <property type="protein sequence ID" value="ABA04380"/>
        <property type="gene ID" value="Nwi_1118"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1118"/>
    <dbReference type="eggNOG" id="ENOG5032ARK">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165466_0_0_5"/>
    <dbReference type="OMA" id="CARRIRW"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="121" mass="13015" checksum="761B00C22BC14505" modified="2005-10-11" version="1">MICARRIRWLYIRDVPQSEIAGASMNLAVHVLLVVAVCLIGLSDTAQARHRHRPVYMFMPWAPVVTPMPPQRLPLVDTGVINQPGVPNTSRPGTIGTGALPSGLKGDSPTAPGFPGWVGRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STL9</accession>
    <name>Q3STL9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rod-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1110"/>
    <dbReference type="EnsemblBacteria" id="ABA04372">
        <property type="protein sequence ID" value="ABA04372"/>
        <property type="gene ID" value="Nwi_1110"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1110"/>
    <dbReference type="eggNOG" id="COG3951">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155700_2_0_5"/>
    <dbReference type="OMA" id="QYSKNFA"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019301">
        <property type="entry name" value="Flagellar_prot_FlgJ_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10135">
        <property type="entry name" value="Rod-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rod-binding" evidence="1">
        <location>
            <begin position="61"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="129" mass="13998" checksum="4DF7E5F040F044E2" modified="2005-10-11" version="1">METHAVNGPLSGAYHNIQRMAPTLNRRPDPQLAQALERVPPQSVEKARATSEDFEAMFLNSMFSQMTTGLKGEGPFGDTPGTGVWRSMLTEQYSRSFAKAGGVGISDAVFRTLIMQQANQTALPPQEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STQ6</accession>
    <name>Q3STQ6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_1073</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1073"/>
    <dbReference type="EnsemblBacteria" id="ABA04335">
        <property type="protein sequence ID" value="ABA04335"/>
        <property type="gene ID" value="Nwi_1073"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1073"/>
    <dbReference type="eggNOG" id="COG0392">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048072_2_1_5"/>
    <dbReference type="OMA" id="WGVREAS"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022791">
        <property type="entry name" value="L-PG_synthase/AglD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03706">
        <property type="entry name" value="LPG_synthase_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="218"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="249"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="291"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="330" mass="35489" checksum="0B25EFD0615D2CE9" modified="2005-10-11" version="1">MANLTAAQTSAMRQILLSTAKILISAALLYLALRKVDISDLLSRLGFTSAVWIALAIAATLSQVFASALRWREISADCGASLSVAQAVRYCMIGSFFNQTLPSSIGGDAMRLWLVGRGPAGWRAAAYSVFVDRAIGLVALGLLIAASLPWSLRLINDPQGRLALLFVDLVALAAAGGFLLLGRLHWRWLGRIRAVHHLRTCSVITNRAIFNRERWPKITFLSLLIHFLAVVIAWCVVKAIAAPVLFSEIFLLVPPVMLITMLPISIAGWGVREASMALTFGYAGLATGEGVNVSLLYGAVFLVVGALGGLVWIFSAERSRKGSIAVESTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="138" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STT3</accession>
    <name>Q3STT3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase</fullName>
            <ecNumber evidence="7">6.3.2.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Meso-A2pm-adding enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Meso-diaminopimelate-adding enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">UDP-MurNAc-tripeptide synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">UDP-N-acetylmuramyl-tripeptide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">murE</name>
        <name evidence="12" type="ordered locus">Nwi_1046</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate</text>
            <dbReference type="Rhea" id="RHEA:23676"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
            <dbReference type="ChEBI" id="CHEBI:83900"/>
            <dbReference type="ChEBI" id="CHEBI:83905"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7 8">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="7">Carboxylation is probably crucial for Mg(2+) binding and, consequently, for the gamma-phosphate positioning of ATP.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the MurCDEF family. MurE subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.13" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314342.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1046"/>
    <dbReference type="EnsemblBacteria" id="ABA04308">
        <property type="protein sequence ID" value="ABA04308"/>
        <property type="gene ID" value="Nwi_1046"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1046"/>
    <dbReference type="eggNOG" id="COG0769">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022291_3_1_5"/>
    <dbReference type="OMA" id="CFMEVSS"/>
    <dbReference type="OrthoDB" id="1861122at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008765">
        <property type="term" value="F:UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00208">
        <property type="entry name" value="MurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000713">
        <property type="entry name" value="Mur_ligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035911">
        <property type="entry name" value="MurE/MurF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005761">
        <property type="entry name" value="UDP-N-AcMur-Glu-dNH2Pim_ligase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01225">
        <property type="entry name" value="Mur_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63418">
        <property type="entry name" value="SSF63418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01085">
        <property type="entry name" value="murE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 7 8" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 7 8" id="KW-0133">Cell shape</keyword>
    <keyword evidence="6 7 8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 7 8" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase" evidence="9">
        <location>
            <begin position="22"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_M" evidence="11">
        <location>
            <begin position="104"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_C" evidence="10">
        <location>
            <begin position="332"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="UDP-MurNAc-L-Ala-D-Glu binding" evidence="7">
        <location>
            <begin position="148"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Meso-diaminopimelate binding" evidence="7">
        <location>
            <begin position="406"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Meso-diaminopimelate recognition motif" evidence="7">
        <location>
            <begin position="406"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:83900"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="106"/>
            <end position="112"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:83900"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:83900"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:83900"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="382"/>
        </location>
        <ligand>
            <name>meso-2,6-diaminoheptanedioate</name>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="454"/>
        </location>
        <ligand>
            <name>meso-2,6-diaminoheptanedioate</name>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="458"/>
        </location>
        <ligand>
            <name>meso-2,6-diaminoheptanedioate</name>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="7">
        <location>
            <position position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA04308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="485" mass="50802" checksum="5A5322BD40DAC84A" modified="2005-10-11" version="1">MKLRELFGDDATMDAPAGELAVTGLAADSRAVKPGDLFFALAGSKTDGARFIDAAIASGAVAVAGDHAPARGRVPFIATPNPRRALALAAAKFYPRQPATIAAVTGTSGKTSVAAFTRQIWQRLGYLSASIGTVGVVAPSRAVYGSVTTPDPIELHRELDQLAGEGVTHLALEASSHGLDQYRVDGVRVRAAGFTNLSRDHMDYHPTVAHYLETKLRLFRDLVESDGIAVICADHDCSDTVIEAAGARNVPVMRVGRRGDGARMGIRLVEAAIDGFAQRVTLEHAGRERVIRLPLVGEFQIENALVSAGLVIGTGGDVDQAFAALEHLEGARGRIERIGERNGAQVFVDYAHKPDALAKALQALRPYAKRKLVVVFGAGGDRDAGKRPLMGAIAADNADVVIVTDDNPRSEDPAAIRAAILAAAPGAREIGDRAEAIGTAIAGLQSGDALLIAGKGHETGQIVGDRVLPFSDHEAASAALTSRRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STV5</accession>
    <name>Q3STV5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Universal stress protein, UspA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1024</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314320.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1024"/>
    <dbReference type="EnsemblBacteria" id="ABA04286">
        <property type="protein sequence ID" value="ABA04286"/>
        <property type="gene ID" value="Nwi_1024"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1024"/>
    <dbReference type="eggNOG" id="COG0589">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_5_2_5"/>
    <dbReference type="OMA" id="EICADVP"/>
    <dbReference type="OrthoDB" id="2040681at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Usp" evidence="2">
        <location>
            <begin position="158"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="279" mass="30175" checksum="0F045C6AC81285D3" modified="2005-10-11" version="1">MAFKDVLLTLMSYPEPTPVSVADDAVSVAAALGAHLAAISCEVHLEAPGNFLAKAIIDVPGIVAHESAKSRENAQGLLAALEAAAARSGVSHEAILEKCAAHKTPAMLVEYARLRDLTIMPEPEAFDLWYAEEIIFESGRPTLILPTVPLSRPIQFGSIGVAWDFSRTATRAVSDALPLLEKAEKVCIFIVRNEKSFNSQRSPDELAENLARHGIDAVLDEVDADGRPIGEVFRAYVLSRGIDLLVMGAYGQSRWREFVLGGATRSLLSKPPVPVFFSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STW4</accession>
    <name>Q3STW4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transposase, IS4 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0180</name>
        <name evidence="4" type="ordered locus">Nwi_1015</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="4">Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0180"/>
    <dbReference type="EnsemblBacteria" id="ABA03448">
        <property type="protein sequence ID" value="ABA03448"/>
        <property type="gene ID" value="Nwi_0180"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04277">
        <property type="protein sequence ID" value="ABA04277"/>
        <property type="gene ID" value="Nwi_1015"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0180"/>
    <dbReference type="KEGG" id="nwi:Nwi_1015"/>
    <dbReference type="eggNOG" id="COG3293">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055261_9_1_5"/>
    <dbReference type="OMA" id="MRSDKTD"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE_Tnp_1" evidence="2">
        <location>
            <begin position="33"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="176" mass="19340" checksum="838E23F8FE482410" modified="2005-10-11" version="1">MGTGVLHPDSRSRQPVSDARLNHRSSPSAGGYRKRGAKDQALGRSRGGLTTKVHMLADALGRPLRFIVTAGQVGDITQAPALLEAQAGDAVLADKAYDSNALRALIAGMGAEAVIPSNRTRKIIIPHDAGLYKHRNRIERCFNRLKHFRRFATRYDRRTVHFTGFVHLAAALIWLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STZ3</accession>
    <name>Q3STZ3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Pyruvate kinase</fullName>
            <ecNumber evidence="4 12">2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">Nwi_0986</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + pyruvate = ADP + H(+) + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the pyruvate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="4 12"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314287.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0986"/>
    <dbReference type="EnsemblBacteria" id="ABA04248">
        <property type="protein sequence ID" value="ABA04248"/>
        <property type="gene ID" value="Nwi_0986"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0986"/>
    <dbReference type="eggNOG" id="COG0469">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015439_0_2_5"/>
    <dbReference type="OMA" id="MVRVHHL"/>
    <dbReference type="OrthoDB" id="1098983at2"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00188"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004743">
        <property type="term" value="F:pyruvate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.33.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001697">
        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018209">
        <property type="entry name" value="Pyrv_Knase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015793">
        <property type="entry name" value="Pyrv_Knase_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015806">
        <property type="entry name" value="Pyrv_Knase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PTHR11817"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01050">
        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="SSF50800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="SSF52935"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01064">
        <property type="entry name" value="pyruv_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00110">
        <property type="entry name" value="PYRUVATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 12" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7 12 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 15" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PK" evidence="13">
        <location>
            <begin position="5"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="PK_C" evidence="14">
        <location>
            <begin position="355"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABA04248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="478" mass="51368" checksum="BD3849A209D81AF6" modified="2005-10-11" version="1">MRRLRRIKILATLGPASSDSTMVRRLFEAGADVFRINMSHTSHDKMRELVATIRNVEASYGRPIGILVDLQGPKLRLGSFADGPVQLSNGETFVLDSDNTPGDKTRVHLPHPEILAALRPGHTLLLDDGKVRLIAEETSPERTVTRVVVGGRMSDRKGVSLPDTDLPVSAMTPKDRSDLDAALETGVDWIALSFVQRADDVAEAKKVIRGRASVMAKIEKPQAIDRLPEIIEMADALMVARGDLGVELPLEQVPSLQKQMTRMARRAGKPVVIATQMLESMILSPVPTRAEVSDVATAVYEGADAIMLSAESAAGKYPVEAISTMNRIGEEVERDPIYRGVLNAQRAEPEPTVGDAIADAARQIAETLDLSAIICWTSSGSTALRMARERPKPPVVAITPNLATGRKLAVAWGVHCVVAEDAHDQDDMVDRAGSIAFRDGFAKAGERIIIVAGVPLGTPGATNMVRIAFVGPNGETGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU12</accession>
    <name>Q3SU12_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0967</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04229">
        <property type="protein sequence ID" value="ABA04229"/>
        <property type="gene ID" value="Nwi_0967"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0967"/>
    <dbReference type="HOGENOM" id="CLU_1853111_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="138" mass="15743" checksum="AB586C566D4EE8DD" modified="2005-10-11" version="1">MLQRGFRLILSDGDFADDEFSADAVTSARFNPHGDLRWLATSGQSKTEVLRFGFATGGEGLLWEDAEPPDLTFLGSARDQDDAFDRAGYRGLETRDRDDQPSGVVLWRLARRHDRDPRRRVDARARVILFCCRHFEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU54</accession>
    <name>Q3SU54_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0925</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314228.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0925"/>
    <dbReference type="EnsemblBacteria" id="ABA04187">
        <property type="protein sequence ID" value="ABA04187"/>
        <property type="gene ID" value="Nwi_0925"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0925"/>
    <dbReference type="eggNOG" id="ENOG5033F5F">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2521063_0_0_5"/>
    <dbReference type="OMA" id="AAWIMVQ"/>
    <dbReference type="OrthoDB" id="2035154at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="86" mass="9191" checksum="F622358FE896E872" modified="2005-10-11" version="1">MSSQPASSASRKIAWPSVITVISAAILIGAEVFGAAFAGGWALAILFGLSEMESHILQAVLFVIGVFVMINFIRAAQRIEPFIRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU77</accession>
    <name>Q3SU77_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sigma70_r2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0902</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04164">
        <property type="protein sequence ID" value="ABA04164"/>
        <property type="gene ID" value="Nwi_0902"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0902"/>
    <dbReference type="HOGENOM" id="CLU_1738594_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="2">
        <location>
            <begin position="77"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="150" mass="16396" checksum="81154243748F665A" modified="2005-10-11" version="1">MPLSAWFGDQCERDGDASAEVAGKRVLLRSSASIPPSRGDPQQSRDFGPSEQPARPQAFCLVSNGGERLMPWDFHTLFMRHSKEIANALRRRGVSPENAADLTQEAFLRLLTAGRPDQTHAIIPAPFFSAPLAICISIRSGASDARRKLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU95</accession>
    <name>Q3SU95_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Class II aldolase/adducin</fullName>
            <ecNumber evidence="2">4.1.2.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0882</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.1.2.17" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314187.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0882"/>
    <dbReference type="EnsemblBacteria" id="ABA04146">
        <property type="protein sequence ID" value="ABA04146"/>
        <property type="gene ID" value="Nwi_0882"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0882"/>
    <dbReference type="eggNOG" id="COG0235">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006033_1_2_5"/>
    <dbReference type="OMA" id="GREAHDW"/>
    <dbReference type="OrthoDB" id="599627at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008738">
        <property type="term" value="F:L-fuculose-phosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="SSF53639"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldolase_II" evidence="1">
        <location>
            <begin position="32"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="259" mass="28537" checksum="6223233A96EFF069" modified="2005-10-11" version="1">MSTMTAPKDELVKLAESRMKVAIAENTWTDRQKLALTCRILFEGGHDSGLAGQITCRAEAPGTYYTQRLGLGFDEIRAQNLLLVDEDLNVLDGGGMANPANRFHSWLYRARPDVNCIIHTHPLHIAALSTLEVPLVVSHMDLCPLYGDVAFLEKWPGVPVGNEEGEIIAEAIGDKKAILLSHHGQLIAGATIEEACVLAMLIERAAKMQLLAMAAGEIKPIPENLAREAHDWISKPKRHSAAFDYYSRRVLGRHNDCLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU97</accession>
    <name>Q3SU97_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Molybdenum ABC transporter, periplasmic binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0880</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314185.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0880"/>
    <dbReference type="EnsemblBacteria" id="ABA04144">
        <property type="protein sequence ID" value="ABA04144"/>
        <property type="gene ID" value="Nwi_0880"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0880"/>
    <dbReference type="eggNOG" id="COG0725">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083611_0_0_5"/>
    <dbReference type="OMA" id="SGDYTWQ"/>
    <dbReference type="OrthoDB" id="1506401at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229131" evidence="1">
        <location>
            <begin position="24"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="285" mass="30422" checksum="5BAD34BBD35238A5" modified="2005-10-11" version="1">MRIRAVSAACAGILILTSVEAYCADAQEQAATLRVFAAGSTRGVLAAIADDYTKATGQRFELTFGPAGLLLNRIQKEGGADVYVSANMEHPQRLYEKGAGTPPVIFARNRLCVTGRAELGLTQDTVLDKLLDPSIKIGTSTPGADPGGDYALQFFDRAETVRAGAKEALIKNARQLVGGPTSPKVPAGETAIHYFFSRHEADVFIGYCSSHTNIGAGTKKPSDVPITRVEVPQPLAIPINYGLTIPATSENEDRMESAYRFALYLMSPDAQRRLPEYGFLAGGGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUB8</accession>
    <name>Q3SUB8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0859</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0859"/>
    <dbReference type="EnsemblBacteria" id="ABA04123">
        <property type="protein sequence ID" value="ABA04123"/>
        <property type="gene ID" value="Nwi_0859"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0859"/>
    <dbReference type="eggNOG" id="COG3504">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1641960_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06911">
        <property type="entry name" value="VirB9_CagX_TrbG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033645">
        <property type="entry name" value="VirB9/CagX/TrbG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038161">
        <property type="entry name" value="VirB9/CagX/TrbG_C_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="161" mass="18179" checksum="15E20669717ADCE9" modified="2005-10-11" version="1">MLAIFSPHAASIFSITYAVLRYLTQAQLKSQHSLLFFGASQYAGRFARCVRQPRHVGTDRCRVYDRAARGLDRDAVDRMASWLSAATRRALVSLAGWPVYPPPAFGPSGNSELVNYRARQNYYVVDWLFAAAELRLGDKDSEKRVRIVRTDGRPGRGPRLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUP5</accession>
    <name>Q3SUP5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Type IV secretory pathway VirD4 component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0730</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314050.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0730"/>
    <dbReference type="EnsemblBacteria" id="ABA03996">
        <property type="protein sequence ID" value="ABA03996"/>
        <property type="gene ID" value="Nwi_0730"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0730"/>
    <dbReference type="eggNOG" id="COG3505">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_603834_0_0_5"/>
    <dbReference type="OMA" id="ELAWVSW"/>
    <dbReference type="OrthoDB" id="385626at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032689">
        <property type="entry name" value="TraG-D_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12696">
        <property type="entry name" value="TraG-D_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TraG-D_C" evidence="1">
        <location>
            <begin position="301"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="459" mass="51425" checksum="944DF5433E57542B" modified="2005-10-11" version="1">MNGLARSYAHIRQGIELGFFRGRPLIYDGTEPVAIDAQAGKGKLTRFLGVNLVSPRTAHLSKIITDPKDAELAWVSWKTLERQGYHVRFINAGRLYGYPSESYNFNTRLLEIASIPALRTLVGEAAYDAASYLVPVDPNPHHKWIGQGVRTAFALYNKITALYPSERWPCTVGGLWDFFGRAPDEIADDLLVWASDSRMEEDWGMCRLVAGLTASTDQWNAYSSTVVERLQGFQPGSAARTVTERNSFDPADMKREKTALFIIGSARSDTSRNFVGAMAAAVIERFADAHGTLRALVVGEEWGQLYVSNFPEILTLYRQGGINFLGVFQNAAAQIENRYGRELARIWRKAVAHTLYRGLPDTETLKDIEHRSGRTSVMVRGFNVNQNQVNGSGDNLSEQSRPLLQVEDIRLATGGESALLESRDQGYFTVDMPNFWERPEMSGMLRDVREKPDKYAWLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUP6</accession>
    <name>Q3SUP6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0729</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0729"/>
    <dbReference type="EnsemblBacteria" id="ABA03995">
        <property type="protein sequence ID" value="ABA03995"/>
        <property type="gene ID" value="Nwi_0729"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0729"/>
    <dbReference type="HOGENOM" id="CLU_1516367_0_0_5"/>
    <dbReference type="OMA" id="VECIPTH"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="79"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="177" mass="19877" checksum="E452673A5E426F52" modified="2005-10-11" version="1">MSSKNNDEMTGIVMVFALIGGMVYAAILAVGFLIILGVAVVTIGFTILSLIAWNRPVRAFGQVITPEEARRFIKQGLHGLWMFPFALALLECFTSLRVNWDYLWVYLFIGYAMGSLGVECIPTHDENGQPLIEILPPDYQPPRQEVLLPPAREPRPAPEPEPEPFRFASWDDEERAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV28</accession>
    <name>Q3SV28_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Flagellar motor switch protein FliN</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_0596</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">FliN is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the motor that determine the direction of flagellar rotation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
            <orientation evidence="8">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Bacterial flagellum basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the FliN/MopA/SpaO family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313924.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0596"/>
    <dbReference type="EnsemblBacteria" id="ABA03863">
        <property type="protein sequence ID" value="ABA03863"/>
        <property type="gene ID" value="Nwi_0596"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0596"/>
    <dbReference type="eggNOG" id="COG1886">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097058_5_1_5"/>
    <dbReference type="OMA" id="MTEIIKT"/>
    <dbReference type="OrthoDB" id="1875967at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012826">
        <property type="entry name" value="FliN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001543">
        <property type="entry name" value="FliN-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001172">
        <property type="entry name" value="FliN_T3SS_HrcQb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036429">
        <property type="entry name" value="SpoA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01052">
        <property type="entry name" value="FliMN_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00956">
        <property type="entry name" value="FLGMOTORFLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101801">
        <property type="entry name" value="SSF101801"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02480">
        <property type="entry name" value="fliN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="4 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="6 8" id="KW-0283">Flagellar rotation</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FliMN_C" evidence="10">
        <location>
            <begin position="41"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA03863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="116" mass="12422" checksum="9E2092D2FD02CC75" modified="2005-10-11" version="1">MSDNDTQVPLPDLNAADAPSIDDVGYSEDEQVSRVASDLEAVFDVPVQVSAVLGRSRMDVGALLKLGPGSVLELDRRVGEAIDIYVNNRLVARGEVVLVEDKLGVTMTEIIKAEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV40</accession>
    <name>Q3SV40_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chorismate mutase</fullName>
            <ecNumber evidence="1">5.4.99.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0584</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="5.4.99.5" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313912.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0584"/>
    <dbReference type="EnsemblBacteria" id="ABA03851">
        <property type="protein sequence ID" value="ABA03851"/>
        <property type="gene ID" value="Nwi_0584"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0584"/>
    <dbReference type="eggNOG" id="COG1605">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065776_0_0_5"/>
    <dbReference type="OMA" id="IWRVIIA"/>
    <dbReference type="OrthoDB" id="1280729at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004106">
        <property type="term" value="F:chorismate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046417">
        <property type="term" value="P:chorismate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.59.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036263">
        <property type="entry name" value="Chorismate_II_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036979">
        <property type="entry name" value="CM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002701">
        <property type="entry name" value="CM_II_prokaryot"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01817">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00830">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48600">
        <property type="entry name" value="SSF48600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51168">
        <property type="entry name" value="CHORISMATE_MUT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chorismate mutase" evidence="2">
        <location>
            <begin position="5"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="284" mass="30842" checksum="4A0E9554468A8DF4" modified="2005-10-11" version="1">MTKPSPAPRSLQELRKEIDAIDEQVHNLLMRRGDIIDRLISVKQTQEVGSAFRPAREADMMRRLAQRHRGILPLDTVESIWRVIIATFTYVQAPFSLHADLSLGESAMRDSARFHFGFTIPFVPHFNAQAAVEAVANAKGDLGLVSATSSRTPWWLALEADGAPRIIARLPFIERADHPAALPVFVISRVADDARVTEVETWSVRVSGWNANTARTLSPLAEIVAVPDTAFDGAALLVSITGDTTLDTIKSALIEAGASVRSTALVGSHATRYTMPSSGGADRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV56</accession>
    <name>Q3SV56_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0568</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313896.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03835">
        <property type="protein sequence ID" value="ABA03835"/>
        <property type="gene ID" value="Nwi_0568"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0568"/>
    <dbReference type="eggNOG" id="ENOG503371U">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119067_0_0_5"/>
    <dbReference type="OMA" id="DKAMPAQ"/>
    <dbReference type="OrthoDB" id="1513998at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229158" evidence="1">
        <location>
            <begin position="25"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="198" mass="20722" checksum="AB9A6E4E600C9510" modified="2005-10-11" version="1">MRDIARSTVIAAAVAGLTLSVAVAVGDSAFAQTKQPPTASEASTPAEKPIKQLALTEKQIEGVLAAQKDMAALDDKLPESSNVEPDGKAAAELEAVSRKAGFASYAEYNDVIDNISLVLAGFDPATKKYIGSDAVLKQQIAAVKSDTKMPSKDKKQALDDMKAALQTPAPAIEKKGNIDLVGKYYDKLVGALSGDEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV73</accession>
    <name>Q3SV73_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0551</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313879.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0551"/>
    <dbReference type="EnsemblBacteria" id="ABA03818">
        <property type="protein sequence ID" value="ABA03818"/>
        <property type="gene ID" value="Nwi_0551"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0551"/>
    <dbReference type="HOGENOM" id="CLU_2650786_0_0_5"/>
    <dbReference type="OMA" id="MRLKFTQ"/>
    <dbReference type="OrthoDB" id="2177326at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="76" mass="8465" checksum="28EAF3CD93A2003B" modified="2005-10-11" version="1">MRLKFTQHPSDYLPQIQHADLSDIEDAPDSADSIADSITLLKDENDRLRKMAASLSVEAEDIRRSLSPAKSTILLV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVB0</accession>
    <name>OTC_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ornithine carbamoyltransferase</fullName>
            <shortName evidence="2">OTCase</shortName>
            <ecNumber evidence="2">2.1.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">argF</name>
        <name type="ordered locus">Nwi_0514</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>carbamoyl phosphate + L-ornithine = H(+) + L-citrulline + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19513"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313842.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVB0"/>
    <dbReference type="SMR" id="Q3SVB0"/>
    <dbReference type="STRING" id="323098.Nwi_0514"/>
    <dbReference type="EnsemblBacteria" id="ABA03781">
        <property type="protein sequence ID" value="ABA03781"/>
        <property type="gene ID" value="Nwi_0514"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0514"/>
    <dbReference type="eggNOG" id="COG0078">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043846_3_2_5"/>
    <dbReference type="OMA" id="DGNNVCN"/>
    <dbReference type="OrthoDB" id="988597at2"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00112"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004585">
        <property type="term" value="F:ornithine carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01109">
        <property type="entry name" value="OTCase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002292">
        <property type="entry name" value="Orn/put_carbamltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024904">
        <property type="entry name" value="OTCase_ArgI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00102">
        <property type="entry name" value="OTCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="SSF53671"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00658">
        <property type="entry name" value="orni_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000084856" description="Ornithine carbamoyltransferase">
        <location>
            <begin position="1"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="56"/>
            <end position="59"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="134"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="229"/>
            <end position="230"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="264"/>
            <end position="265"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01109"/>
        </source>
    </evidence>
    <sequence length="308" mass="33752" checksum="5D0CB37EB556A94E" modified="2005-10-11" version="1">MSKAPRHFLDLLDMSTGELRAILDASVAMKKRRKEGLIADKPLAGKTLAMIFDKPSTRTRVSFDVGMRQLGGEAIMLTGAEMQLGRGETIADTAKVLSRFVDIIMIRILSHDALTELAAHATVPVINGLTRRSHPCQVMADVMTFEEHRGPIEGRTVAWTGDDNNVLASWAHAAERFSFNLNVATPQELAPNKPLKDWIRSSGASIRLGTDPEAAVRGADCIVTDTWVSMGDKDGEHRHNLLRPYQVNAELMRLAKSDALFMHCLPAHRGEEVTDEVIDGPQSVVFDEAENRLHAQKGILAWCLGAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVH8</accession>
    <name>Q3SVH8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosomal silencing factor RsfS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rsfS</name>
        <name evidence="3" type="ordered locus">Nwi_0446</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Functions as a ribosomal silencing factor. Interacts with ribosomal protein L14 (rplN), blocking formation of intersubunit bridge B8. Prevents association of the 30S and 50S ribosomal subunits and the formation of functional ribosomes, thus repressing translation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with ribosomal protein L14 (rplN).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the Iojap/RsfS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0446"/>
    <dbReference type="EnsemblBacteria" id="ABA03713">
        <property type="protein sequence ID" value="ABA03713"/>
        <property type="gene ID" value="Nwi_0446"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0446"/>
    <dbReference type="eggNOG" id="COG0799">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092688_6_0_5"/>
    <dbReference type="OMA" id="VVHVFQK"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:mature ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090071">
        <property type="term" value="P:negative regulation of ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01477">
        <property type="entry name" value="Iojap_RsfS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004394">
        <property type="entry name" value="Iojap/RsfS/C7orf30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21043">
        <property type="entry name" value="PTHR21043"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00090">
        <property type="entry name" value="rsfS_iojap_ybeB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="2" id="KW-0810">Translation regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="123" mass="13750" checksum="4915C900AEBA6765" modified="2005-10-11" version="1">MAPLSPEAQPDVEQTLRLILSRLEDMKAEDAVTIGLRGRSAFSDYMVVVTGRSNRHVGAIAETVARGLKDIGVKKIRIEGMPNCDWVLIDSGDVVVHVFRPEVREFYNLERLWIQNTATAKTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVH9</accession>
    <name>Q3SVH9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Probable nicotinate-nucleotide adenylyltransferase</fullName>
            <ecNumber evidence="7">2.7.7.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Deamido-NAD(+) diphosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Deamido-NAD(+) pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Nicotinate mononucleotide adenylyltransferase</fullName>
            <shortName evidence="7">NaMN adenylyltransferase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">nadD</name>
        <name evidence="9" type="ordered locus">Nwi_0445</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 7">Catalyzes the reversible adenylation of nicotinate mononucleotide (NaMN) to nicotinic acid adenine dinucleotide (NaAD).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP + H(+) + nicotinate beta-D-ribonucleotide = deamido-NAD(+) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22860"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="EC" id="2.7.7.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 7">Cofactor biosynthesis; NAD(+) biosynthesis; deamido-NAD(+) from nicotinate D-ribonucleotide: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 7">Belongs to the NadD family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.18" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0445"/>
    <dbReference type="EnsemblBacteria" id="ABA03712">
        <property type="protein sequence ID" value="ABA03712"/>
        <property type="gene ID" value="Nwi_0445"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0445"/>
    <dbReference type="eggNOG" id="COG1057">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069765_2_0_5"/>
    <dbReference type="OMA" id="IHIGHLI"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00332"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004515">
        <property type="term" value="F:nicotinate-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02165">
        <property type="entry name" value="NMNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00244">
        <property type="entry name" value="NaMN_adenylyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005248">
        <property type="entry name" value="NadD/NMNAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00482">
        <property type="entry name" value="TIGR00482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CTP_transf_like" evidence="8">
        <location>
            <begin position="22"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA03712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="210" mass="23318" checksum="D344C3C5C8215F24" modified="2005-10-11" version="1">MGSRSVVSRAIPLHSDGMRIGLLGGSFNPPHAAHRAVSLYALKRLELDRVWWLVSPANPLKDARALRALGERAAAASAVANDPRIDISCLEAVIGTRYTIDTITYLRRRCANVRFVWIMGADNLEQFHRWENWRRIAAAAPIAVVDRPPHSFQALAAPAAQALARWRLPEARADRLASHRLPAWVFLTGMKSRLSSTGLRNQDGTWRTGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVI3</accession>
    <name>Q3SVI3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GCN5-related N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0441</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313772.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0441"/>
    <dbReference type="EnsemblBacteria" id="ABA03708">
        <property type="protein sequence ID" value="ABA03708"/>
        <property type="gene ID" value="Nwi_0441"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0441"/>
    <dbReference type="eggNOG" id="COG1670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_3_4_5"/>
    <dbReference type="OMA" id="VPTWTYL"/>
    <dbReference type="OrthoDB" id="1538300at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="33"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="202" mass="22893" checksum="A634F3ABC8FE67E6" modified="2005-10-11" version="1">MLQDMPIPTPLLRDRRPFALETERLTLRKPTHADVRGITALANDRRIAEMTRCLPHPYTPDDAVQFVEAVAGDSREMVFLVEHNHVPVGVAGVDWSKPDAPELGYWFGVDHWGRGFATEAVRAVIDYAFEEYGVDRMVSGARVINPASRNVLEKCGFQWTGVELYRIRSLGSSTPVDRFRLSRGVWSSLKRWNSAARREPCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVP9</accession>
    <name>Q3SVP9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4167 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0375</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313706.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0375"/>
    <dbReference type="EnsemblBacteria" id="ABA03642">
        <property type="protein sequence ID" value="ABA03642"/>
        <property type="gene ID" value="Nwi_0375"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0375"/>
    <dbReference type="eggNOG" id="COG5373">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069113_1_0_5"/>
    <dbReference type="OMA" id="QEQFRQN"/>
    <dbReference type="OrthoDB" id="1736914at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025430">
        <property type="entry name" value="DUF4167"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13763">
        <property type="entry name" value="DUF4167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4167" evidence="2">
        <location>
            <begin position="12"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="231" mass="26362" checksum="D00E214A2EB4A0E2" modified="2005-10-11" version="1">MRNGQNNNKRMRNRNNNNNNNRRGQNPMTRVFESNGPDIKIRGTASHVAEKYLQLARDARSSGDPVAAENYYQHAEHYFRLIAAAQEQFRQSQPQQQPRIDVEATDEAGEDDSESFSNFGAEPGFVPVQQPRDHHHREAQPYQRDQQQPREYRQPQPVIQPADNGGVDRLPSFITGSQPQVNGGRSDSNGHQGGERNDRFPPRRRRRPHNPRPESMAQPPAPSEDFNPGNE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-01-09" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVV2</accession>
    <name>CCMA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cytochrome c biogenesis ATP-binding export protein CcmA</fullName>
            <ecNumber evidence="1">7.6.2.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Heme exporter protein A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ccmA</name>
        <name type="ordered locus">Nwi_0322</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the ABC transporter complex CcmAB involved in the biogenesis of c-type cytochromes; once thought to export heme, this seems not to be the case, but its exact role is uncertain. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + heme b(in) = ADP + H(+) + heme b(out) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19261"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.6.2.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of two ATP-binding proteins (CcmA) and two transmembrane proteins (CcmB).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. CcmA exporter (TC 3.A.1.107) family.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.5" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313654.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVV2"/>
    <dbReference type="SMR" id="Q3SVV2"/>
    <dbReference type="STRING" id="323098.Nwi_0322"/>
    <dbReference type="EnsemblBacteria" id="ABA03589">
        <property type="protein sequence ID" value="ABA03589"/>
        <property type="gene ID" value="Nwi_0322"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0322"/>
    <dbReference type="eggNOG" id="COG4133">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_5"/>
    <dbReference type="OMA" id="NLAWLCA"/>
    <dbReference type="OrthoDB" id="1833499at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015439">
        <property type="term" value="F:ABC-type heme transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005895">
        <property type="entry name" value="ABC_transptr_haem_export_CcmA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43499">
        <property type="entry name" value="PTHR43499"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01189">
        <property type="entry name" value="ccmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51243">
        <property type="entry name" value="CCMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000271935" description="Cytochrome c biogenesis ATP-binding export protein CcmA">
        <location>
            <begin position="1"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="1"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01707"/>
        </source>
    </evidence>
    <sequence length="200" mass="20463" checksum="C67C9B27EF420A6C" modified="2005-10-11" version="1">MRLSGNGLRCVRGGREVFSGLDVAAESGHAVAITGPNGAGKTSLLRLLAGLLAIADGSISLEGGDPELTLPEQAHYLGHRDALKPALTVSENLSFWRDFLGGGKSGESDALAAVGLDHVAHLPAAYLSAGQRRRLSIARLLAVKRPVWLLDEPTSALDVTGQAAFAAIMTGHLAGGGIILAATHTPLGIAARELRIGGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVX0</accession>
    <name>Q3SVX0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flp/Fap pilin component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0304</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313637.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0304"/>
    <dbReference type="EnsemblBacteria" id="ABA03571">
        <property type="protein sequence ID" value="ABA03571"/>
        <property type="gene ID" value="Nwi_0304"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0304"/>
    <dbReference type="eggNOG" id="COG3847">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_171854_3_3_5"/>
    <dbReference type="OMA" id="AINAKFT"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007047">
        <property type="entry name" value="Flp_Fap"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04964">
        <property type="entry name" value="Flp_Fap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="55" mass="5876" checksum="84BE49BB397C8D20" modified="2005-10-11" version="1">MKNLFSRFLKDESGATAIEYGLIAAGIAVAIITAVNTLGTSLNTTFTKVEQDLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVY5</accession>
    <name>Q3SVY5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Extracellular solute-binding protein, family 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_0289</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial solute-binding protein 5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313622.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0289"/>
    <dbReference type="EnsemblBacteria" id="ABA03556">
        <property type="protein sequence ID" value="ABA03556"/>
        <property type="gene ID" value="Nwi_0289"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0289"/>
    <dbReference type="eggNOG" id="COG4166">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023171_0_0_5"/>
    <dbReference type="OMA" id="YIEHQAK"/>
    <dbReference type="OrthoDB" id="162819at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030288">
        <property type="term" value="C:outer membrane-bounded periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030678">
        <property type="entry name" value="Peptide/Ni-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PTHR30290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002741">
        <property type="entry name" value="MppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0574">Periplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229293" evidence="4">
        <location>
            <begin position="30"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="SBP_bac_5" evidence="5">
        <location>
            <begin position="127"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA03556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="632" mass="70866" checksum="4A9D1942349C62FE" modified="2005-10-11" version="1">MSRPSRRQVFGLGIGAAGAAWLRPAVAVAANGAEPNPHGESQGQSHGMSAFGDLKYPADFHHFDYVNPDAPKGGLFATIPSSRAFNQSFQTFNSLNAFILKGDGAQGMGMTFTSLMARAGDEPDAMYGLAAKSVRISADGLTYRFTMRPEARFHDGQKITARDAAFSLMVLKTKGHPLITQQVRDMVKAEAPDDATLVVTFAAKRGRDVPLFVAGLPIFSQAYYTARPFDETTLDVPLGSGPYKVGRFEANRFIEFDRVKDWWGADLPVCRGAYNFDTVRFDFYRDRDVAFEGFTGRSYLYREEFTSRIWNTRYDFPAMTDGRVKREQLPDETPSGAQGWFINTRRDKFKDPRVREALDCAFDFEWTNKSIMYGAYVRTVSPFQNSDLMASGPPSQEEVALLEPFRGKVPDEVFGNPYTPLVSDGSGQDRKQLRRAAQLLDEAGFHIKDRKRMTPRGEVFRLEFLLDEPAFQAHHMPYIKNLQTLGIEATLRLVDPVQSRSRRDDFDFDIIIERFSFSTIPGDSLRPFFSSRAAATKGSNNLAGIADPAIDALMEQVIVADTRARLVFAARALDRVIRAGRYWVPQWYSNTHRLAYWDVFAHPPSLPKYLGVMAPDIWWSTQARPASSGQAG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-01-10" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW26</accession>
    <name>HISX_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Histidinol dehydrogenase</fullName>
            <shortName evidence="1">HDH</shortName>
            <ecNumber evidence="1">1.1.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisD</name>
        <name type="ordered locus">Nwi_0247</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the sequential NAD-dependent oxidations of L-histidinol to L-histidinaldehyde and then to L-histidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-histidinol + 2 NAD(+) = 3 H(+) + L-histidine + 2 NADH</text>
            <dbReference type="Rhea" id="RHEA:20641"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:57699"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 9/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the histidinol dehydrogenase family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="ABA03515" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="1.1.1.23" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03515.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_041344641.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SW26"/>
    <dbReference type="SMR" id="Q3SW26"/>
    <dbReference type="STRING" id="323098.Nwi_0247"/>
    <dbReference type="EnsemblBacteria" id="ABA03515">
        <property type="protein sequence ID" value="ABA03515"/>
        <property type="gene ID" value="Nwi_0247"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0247"/>
    <dbReference type="eggNOG" id="COG0141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006732_3_3_5"/>
    <dbReference type="OrthoDB" id="935289at2"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00014"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004399">
        <property type="term" value="F:histidinol dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06572">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01024">
        <property type="entry name" value="HisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001692">
        <property type="entry name" value="Histidinol_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022695">
        <property type="entry name" value="Histidinol_DH_monofunct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012131">
        <property type="entry name" value="Hstdl_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21256">
        <property type="entry name" value="PTHR21256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00815">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000099">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00083">
        <property type="entry name" value="HOLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00069">
        <property type="entry name" value="hisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00611">
        <property type="entry name" value="HISOL_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000135803" description="Histidinol dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="327"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="328"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="361"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="361"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="415"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="420"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="420"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="429" mass="44496" checksum="AED2E80E595F7F0C" modified="2006-01-10" version="2">MPIRIDSRNADFVPRFKAFLATKREVSADIEAATRAIVDDVAARGDAALIEATCKFDRLSVDAAGLRFTAAEIDAAVAACDAATLDALKLARDRIETFHRRQLPKDDRFIDALGVELGSRWTAIEAVGLYVPGGSAAYPSSVLMNAVPAMVAGVARVVMVVPSPAGRINPLVLAAARLGGVSEIYRVGGAQAVAALAYGTATIAPVAKIVGPGNAYVAAAKRQVFGKVGIDMIAGPSEVLVIADQTGNAGWIAADLLAQAEHDANAQSILITDSEALAADVERAVETQLATLPRAGIARASWNDFGAVILVTDLDEAVVLADSIAAEHLEIMTADPEGLAARIRNAGAIFLGPHTPEAIGDYVGGSNHVLPTARSARFSSGLGVLDFMKRTSILKCGPDQLRALGPAAMTLGKAEGLDAHARSVGVRLN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-02-10" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW35</accession>
    <name>ATP6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP synthase subunit a</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP synthase F0 sector subunit a</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">F-ATPase subunit 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpB</name>
        <name type="ordered locus">Nwi_0238</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Key component of the proton channel; it plays a direct role in the translocation of protons across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a(1), b(2) and c(9-12). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. CF(1) is attached to CF(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase A chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313572.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SW35"/>
    <dbReference type="SMR" id="Q3SW35"/>
    <dbReference type="STRING" id="323098.Nwi_0238"/>
    <dbReference type="EnsemblBacteria" id="ABA03506">
        <property type="protein sequence ID" value="ABA03506"/>
        <property type="gene ID" value="Nwi_0238"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0238"/>
    <dbReference type="eggNOG" id="COG0356">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041018_0_2_5"/>
    <dbReference type="OMA" id="FFDQFMS"/>
    <dbReference type="OrthoDB" id="867266at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045263">
        <property type="term" value="C:proton-transporting ATP synthase complex, coupling factor F(o)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01393">
        <property type="entry name" value="ATP_synth_a_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000568">
        <property type="entry name" value="ATP_synth_F0_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023011">
        <property type="entry name" value="ATP_synth_F0_asu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045083">
        <property type="entry name" value="ATP_synth_F0_asu_bact/mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035908">
        <property type="entry name" value="F0_ATP_A_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11410">
        <property type="entry name" value="PTHR11410"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00119">
        <property type="entry name" value="ATP-synt_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00123">
        <property type="entry name" value="ATPASEA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81336">
        <property type="entry name" value="SSF81336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01131">
        <property type="entry name" value="ATP_synt_6_or_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00449">
        <property type="entry name" value="ATPASE_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0138">CF(0)</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000362355" description="ATP synthase subunit a">
        <location>
            <begin position="1"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01393"/>
        </source>
    </evidence>
    <sequence length="247" mass="27009" checksum="1A2ECAB0D0F79AED" modified="2005-10-11" version="1">MMDPIHQFNIEPIFTIGHIGGQEIAFTNSSAYMFLAVALTSLLMLGTGRRLVPGRMQSIAEISYEFVADTIRTTAGKEGMKFFPFVFSIFMLVTVSNLVGIIPYTFTISSHIIVTAALAFLVFFTVLIYGFYKNGLRFFKLFVPSGIPVVILPLVVTIEVISFLSRPVSHSVRLFANMLAGHITLKVFASFVTMLGAMGIVGVFGAVLPLALVVALTALELLVAFLQAYVFTILTCIYINDAIHPGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWE8</accession>
    <name>Q3SWE8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0125</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0125"/>
    <dbReference type="EnsemblBacteria" id="ABA03393">
        <property type="protein sequence ID" value="ABA03393"/>
        <property type="gene ID" value="Nwi_0125"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0125"/>
    <dbReference type="eggNOG" id="ENOG50331PK">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128171_0_0_5"/>
    <dbReference type="OMA" id="MRTYTLH"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024399">
        <property type="entry name" value="DUF2628"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10947">
        <property type="entry name" value="DUF2628"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="84"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="182" mass="19999" checksum="0BB014FE48252C54" modified="2005-10-11" version="1">MLLSAESAMSVYTVHAPPADGPDGRGATDNFVFVRDGFSFGAFVLGPIWLLYRRLWLVLAGYLVLSIAMEVVMTLLRVGAGTRVAAMAVVAVLMGLEAASLRRWTLSRRKWRPLDVVVADDQEAAERRFFDRWQAMRSADYDPLAIDRGAPPPTRGGPDTSLPPSLPRGDIVGLFPQPGFRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWF5</accession>
    <name>Q3SWF5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Mg chelatase-related protein</fullName>
            <ecNumber evidence="3">6.6.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0118</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mg-chelatase subunits D/I family. ComM subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.6.1.1" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313455.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0118"/>
    <dbReference type="EnsemblBacteria" id="ABA03386">
        <property type="protein sequence ID" value="ABA03386"/>
        <property type="gene ID" value="Nwi_0118"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0118"/>
    <dbReference type="eggNOG" id="COG0606">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026145_1_1_5"/>
    <dbReference type="OMA" id="STNPCPC"/>
    <dbReference type="OrthoDB" id="581802at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016851">
        <property type="term" value="F:magnesium chelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045006">
        <property type="entry name" value="CHLI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004482">
        <property type="entry name" value="Mg_chelat-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025158">
        <property type="entry name" value="Mg_chelat-rel_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000523">
        <property type="entry name" value="Mg_chelatse_chII-like_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32039">
        <property type="entry name" value="PTHR32039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32039:SF7">
        <property type="entry name" value="PTHR32039:SF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01078">
        <property type="entry name" value="Mg_chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13335">
        <property type="entry name" value="Mg_chelatase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00368">
        <property type="entry name" value="TIGR00368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="209"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="512" mass="53620" checksum="FB7AF868DC97C842" modified="2005-10-11" version="1">MVQRVSTVAFEGIEARAVDVQVQVAPGLPAFNIVGLPDKAVSEARERVRAALIASGLALPARRITVNLAPADLPKEGSHYDLPIALGLMAAIGAIPSDALAGFTVLGELGLDGSIAAVAGVLPAAIGANAREDGLICPSACGAEAAWASPDIQIIAASSLIQIANHFKGTQVLSRPQPRIRETQGSALDLRDIKGQESAKRALEIAAAGGHHLLMVGSPGSGKSMLAARLPSILPPLSPAELLELSMIASVAGEIDGGALTSQRPFRAPHHSASMAALTGGGTRARPGEISLAHNGVLFLDELPEFDPRVLDSLRQPLENGEIAVSRANHRVTYPARFMLVAAMNPCRCGHAFEPGYACKRGRIDRCTADYQMRISGPLLDRIDLRIEVPAVTASDLILPPSSEGSAEVGARVAAARDIQTERYAALGLSNVRTNAEAPAAALEAIAQPDAQGLKLLHDAAESMRLSARGYHRVLRVARTLADLDGADRIGRLYLAEALSYRALADDIKRAA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-01-10" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWH1</accession>
    <name>PDRP_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative pyruvate, phosphate dikinase regulatory protein</fullName>
            <shortName evidence="1">PPDK regulatory protein</shortName>
            <ecNumber evidence="1">2.7.11.32</ecNumber>
            <ecNumber evidence="1">2.7.4.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">Nwi_0102</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Bifunctional serine/threonine kinase and phosphorylase involved in the regulation of the pyruvate, phosphate dikinase (PPDK) by catalyzing its phosphorylation/dephosphorylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ADP + N(tele)-phospho-L-histidyl/L-threonyl-[pyruvate, phosphate dikinase] = AMP + H(+) + N(tele)-phospho-L-histidyl/O-phospho-L-threonyl-[pyruvate, phosphate dikinase]</text>
            <dbReference type="Rhea" id="RHEA:43692"/>
            <dbReference type="Rhea" id="RHEA-COMP:10650"/>
            <dbReference type="Rhea" id="RHEA-COMP:10651"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:83586"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.32"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + N(tele)-phospho-L-histidyl/O-phospho-L-threonyl-[pyruvate, phosphate dikinase] + phosphate = diphosphate + N(tele)-phospho-L-histidyl/L-threonyl-[pyruvate, phosphate dikinase]</text>
            <dbReference type="Rhea" id="RHEA:43696"/>
            <dbReference type="Rhea" id="RHEA-COMP:10650"/>
            <dbReference type="Rhea" id="RHEA-COMP:10651"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:83586"/>
            <dbReference type="EC" id="2.7.4.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the pyruvate, phosphate/water dikinase regulatory protein family. PDRP subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.32" evidence="1"/>
    <dbReference type="EC" id="2.7.4.27" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313439.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWH1"/>
    <dbReference type="SMR" id="Q3SWH1"/>
    <dbReference type="STRING" id="323098.Nwi_0102"/>
    <dbReference type="EnsemblBacteria" id="ABA03370">
        <property type="protein sequence ID" value="ABA03370"/>
        <property type="gene ID" value="Nwi_0102"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0102"/>
    <dbReference type="eggNOG" id="COG1806">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046206_2_0_5"/>
    <dbReference type="OMA" id="YAQCEFE"/>
    <dbReference type="OrthoDB" id="980472at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016776">
        <property type="term" value="F:phosphotransferase activity, phosphate group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006470">
        <property type="term" value="P:protein dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00921">
        <property type="entry name" value="PDRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005177">
        <property type="entry name" value="Kinase-pyrophosphorylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026565">
        <property type="entry name" value="PPDK_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31756">
        <property type="entry name" value="PTHR31756"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03618">
        <property type="entry name" value="Kinase-PPPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000196680" description="Putative pyruvate, phosphate dikinase regulatory protein">
        <location>
            <begin position="1"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="154"/>
            <end position="161"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00921"/>
        </source>
    </evidence>
    <sequence length="280" mass="31027" checksum="00C9549F21389391" modified="2005-10-11" version="1">MAPGTGRDFHLHLVSDSTGETLITVSRAVTAQYAGVTPIEHVYPLVRSEKQLDRVLAEIEEAPGIVLFTLLEKDVAVRLEARCQEINCPSLSIIGPVMQLFQAYLGADTTGRVGAQHTLNADYFKRIDALNYTMMHDDGQHVESLEEADVVLVGVSRTSKTPTSVYLANRGTRTANVPLVPGIAIPRQLETLKNPLVVSLHAAPERLIQVRQNRLLSIGAGPGNEAYIDRQSVTEEVIHARRLSVKHDWAQLDVTRRSIEETAAAIMKLFADRRRQRRPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWJ9</accession>
    <name>Q3SWJ9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Peptidase S16</fullName>
            <ecNumber evidence="2">3.4.21.53</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0074</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.4.21.53" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313411.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0074"/>
    <dbReference type="EnsemblBacteria" id="ABA03342">
        <property type="protein sequence ID" value="ABA03342"/>
        <property type="gene ID" value="Nwi_0074"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0074"/>
    <dbReference type="eggNOG" id="COG2802">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048359_2_1_5"/>
    <dbReference type="OMA" id="YRQCKVD"/>
    <dbReference type="OrthoDB" id="1474822at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="1">
        <location>
            <begin position="17"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="224" mass="24927" checksum="A8A39D2AD18747C3" modified="2005-10-11" version="1">MPINADYRGPADLPEVIPVFPLPGALLLPRGQMPLNIFEMRYLAMVDDALRDGHRLIGMIQPDLTHSASEDKPELFHVGCAGRITQFAESGDGRYILELTGVSRFKVVEELTVLTPYRQCKVDFFAYADDLTARKGEDEVDRKRLLEVLTDFLKVNNLKVDWNGIENAPNEALVNALAMMSPYGPPEKQAMLEAADLKTRAEILIAVTEMDLAKKRTSGDPGLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWP2</accession>
    <name>Q3SWP2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glutathione S-transferase</fullName>
            <ecNumber evidence="3">2.5.1.18</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0031</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.5.1.18" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313370.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0031"/>
    <dbReference type="EnsemblBacteria" id="ABA03299">
        <property type="protein sequence ID" value="ABA03299"/>
        <property type="gene ID" value="Nwi_0031"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0031"/>
    <dbReference type="eggNOG" id="COG0625">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011226_6_4_5"/>
    <dbReference type="OMA" id="PMSRGRI"/>
    <dbReference type="OrthoDB" id="1383885at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="SSF47616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="2">
        <location>
            <begin position="86"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="198" mass="21754" checksum="DFFA7A5D3E44DAB9" modified="2005-10-11" version="1">MMKLYWSPRSRSFTALWLMEETGQPYERVLIDISTGAQKTPEFLAVNPMGKVPALTDGAIAIAEAAAICAYVAERFPDAKLAPPLGDARRAKYLQWLFFGPSCIEPALLQIFAKLQVPSSTAAWGDATQVFDVLDKAVEKGPWILGDDFSAADIVIGSGLNFAIRQFKMVPPRPAFDRYIDRCVARPAFQRAEKIAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWQ0</accession>
    <name>Q3SWQ0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">YlxR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0023</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0023"/>
    <dbReference type="EnsemblBacteria" id="ABA03291">
        <property type="protein sequence ID" value="ABA03291"/>
        <property type="gene ID" value="Nwi_0023"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0023"/>
    <dbReference type="eggNOG" id="COG2740">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091016_1_0_5"/>
    <dbReference type="OMA" id="TCIVTRK"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00279">
        <property type="entry name" value="YlxR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007393">
        <property type="entry name" value="DUF448"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="L30e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037465">
        <property type="entry name" value="YlxR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035931">
        <property type="entry name" value="YlxR-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34215">
        <property type="entry name" value="PTHR34215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04296">
        <property type="entry name" value="YlxR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="SSF55315"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64376">
        <property type="entry name" value="SSF64376"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YlxR" evidence="1">
        <location>
            <begin position="23"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="221" mass="23547" checksum="07B0AFBDCAA5CECE" modified="2005-10-11" version="1">MLAIADPADLDQGPRTNRSATARMCAVTRQVRPIGELIRFVVSPSGEVIADLKRKLPGRGLWISASHAAVAEAVRRNQFSRGFKRDVRVPASLAEDTRQLMVRSAMDALAMVAKAGQVVAGFAKVEDALLRRQAVALIHAADGAADGIRKLDAAVRKIAGQSGEAREIPVVTALNSAELDLALGRANVIHAALLAGPAGRTFLQRSQTLVRYQDGGQRQDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="128" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMU8</accession>
    <name>Q3SMU8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7 8">DNA replication and repair protein RecF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">recF</name>
        <name evidence="10" type="ordered locus">Nwi_0003</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7 8">The RecF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the RecF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0003"/>
    <dbReference type="EnsemblBacteria" id="ABA03271">
        <property type="protein sequence ID" value="ABA03271"/>
        <property type="gene ID" value="Nwi_0003"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0003"/>
    <dbReference type="eggNOG" id="COG1195">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040267_2_0_5"/>
    <dbReference type="OMA" id="GESWSYA"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00365">
        <property type="entry name" value="RecF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001238">
        <property type="entry name" value="DNA-binding_RecF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018078">
        <property type="entry name" value="DNA-binding_RecF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042174">
        <property type="entry name" value="RecF_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32182:SF0">
        <property type="entry name" value="PTHR32182:SF0"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00611">
        <property type="entry name" value="recf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00617">
        <property type="entry name" value="RECF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00618">
        <property type="entry name" value="RECF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 8" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0742">SOS response</keyword>
    <feature type="domain" description="SMC_N" evidence="9">
        <location>
            <begin position="10"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA03271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="385" mass="41433" checksum="15BC3E58C35B2FE5" modified="2005-10-11" version="1">MPGVMIPSRVRRLSLTHFRNYRAATFETRSNMIVLVGPNGAGKTNCLEAISLLSPGRGLRRATRDDIADNTGDGSWAVSAEMQGALGLATLGTGIDAPGNEAAPSGRRCRIDREPVASAAAFGDHLRMVWLTPSMDGLFTGPASDRRRFLDRLVLAIDSEHSGRVSALERSLRSRNRLLEMRHHDDLWCEAIERKTAELAVAVAAMRAQTVTRLTAALEARGGGSSFPAASIHLDGWMENALLTEPATVVEDRYREILRASRPRDAAAGRTLEGPHLTDLEVIYAPKNMPAKEASTGEQKALLIGLVLAHARLVAEMTGIIPLLLLDEVVAHLDPDRRGALFGELAGLGAQVWMSGADPAAFANLSAGSETFRVDSGRITREPPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMW5</accession>
    <name>Q3SMW5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">YtkA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_3129</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316284.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3129"/>
    <dbReference type="EnsemblBacteria" id="ABA06376">
        <property type="protein sequence ID" value="ABA06376"/>
        <property type="gene ID" value="Nwi_3129"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3129"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1884957_0_0_5"/>
    <dbReference type="OMA" id="GWRFKLA"/>
    <dbReference type="OrthoDB" id="1779000at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032693">
        <property type="entry name" value="YtkA-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13115">
        <property type="entry name" value="YtkA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229042" description="YtkA domain-containing protein" evidence="1">
        <location>
            <begin position="29"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="YtkA" evidence="2">
        <location>
            <begin position="28"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="135" mass="14186" checksum="7575F51574BD7726" modified="2005-10-11" version="1">MITALTKRAVAAVLAAALSVTAFSAAKADITDYAFRLVQTEIKHGNGAIVAVRLIDKRSGKAVPDAVIFATRIDMAPDGMESMAAPIEALPSTEPGVYRFKTNLTMAGGWRLSLGAKIQGETGTLENKLVLKATP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMY1</accession>
    <name>Q3SMY1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase IS3/IS911</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2330</name>
        <name evidence="3" type="ordered locus">Nwi_3113</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="3">Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2330"/>
    <dbReference type="EnsemblBacteria" id="ABA05584">
        <property type="protein sequence ID" value="ABA05584"/>
        <property type="gene ID" value="Nwi_2330"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06360">
        <property type="protein sequence ID" value="ABA06360"/>
        <property type="gene ID" value="Nwi_3113"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2330"/>
    <dbReference type="KEGG" id="nwi:Nwi_3113"/>
    <dbReference type="eggNOG" id="COG2963">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027402_36_0_5"/>
    <dbReference type="OMA" id="CCKDVIA"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002514">
        <property type="entry name" value="Transposase_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010921">
        <property type="entry name" value="Trp_repressor/repl_initiator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01527">
        <property type="entry name" value="HTH_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48295">
        <property type="entry name" value="SSF48295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="81"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="124" mass="13943" checksum="A01C47A19EB06B79" modified="2005-10-11" version="1">MRQKSGPEKAPAEQVVKDIRRATRRQFSAEEKTRIVLEGVRGEESIAELCRREGIASSMYYGWSKEFLDAGKRRLAGDTARAATSDEVKELRREAQALKEAVADLTLENRLLKKSMLADGEDDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMY5</accession>
    <name>Q3SMY5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Resolvase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_3109</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3109"/>
    <dbReference type="EnsemblBacteria" id="ABA06356">
        <property type="protein sequence ID" value="ABA06356"/>
        <property type="gene ID" value="Nwi_3109"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3109"/>
    <dbReference type="eggNOG" id="COG1961">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2948">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_733265_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038109">
        <property type="entry name" value="DNA_bind_recomb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011109">
        <property type="entry name" value="DNA_bind_recombinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006118">
        <property type="entry name" value="Recombinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="SSF53041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51737">
        <property type="entry name" value="RECOMBINASE_DNA_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00397">
        <property type="entry name" value="RECOMBINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="7">
        <location>
            <begin position="22"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Recombinase" evidence="8">
        <location>
            <begin position="178"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="325"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="342"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-serine intermediate" evidence="4 5">
        <location>
            <position position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606118-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA06356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="377" mass="41510" checksum="43CCD152E2FD3F50" modified="2005-10-11" version="1">MIARNLHSRTNLAATDCPAPLRTAVYLRVSTGRQAEGEVSIPSQRDLTTRHCLSNGWSVVDEYVEPGASATDDRRPVFQAMLERACDADHPYDVIVVHSFSRFFRDGATMELTIRKLRRHGVEVVSMTQPTGTDPSQEMMRQIIGIFDEYTSKENGKNVTRAMKENAKQGFWNGASPPLGYTIVEAERRGQKIKKHLAIDPVEAETVRLIFRLYAEGDSETRTPPLGIKQLVKWLNSHGYRTKAGGTFGVGPMHHMHPPRPSLGVGGALIYAIQTRDAGKSGQELYSTTNRQPADGLAGLPRDYTGPVLGPPLPGDLGRPIVDAQNRGRPVVPPTIATPTVDEAEQRRRAEEEAARTTRKCRQLNDPIAESTDVGSV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMZ4</accession>
    <name>LPXD2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UDP-3-O-acylglucosamine N-acyltransferase 2</fullName>
            <ecNumber evidence="1">2.3.1.191</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lpxD2</name>
        <name type="ordered locus">Nwi_3100</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a (3R)-hydroxyacyl-[ACP] + a UDP-3-O-[(3R)-3-hydroxyacyl]-alpha-D-glucosamine = a UDP-2-N,3-O-bis[(3R)-3-hydroxyacyl]-alpha-D-glucosamine + H(+) + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:53836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="ChEBI" id="CHEBI:137740"/>
            <dbReference type="ChEBI" id="CHEBI:137748"/>
            <dbReference type="EC" id="2.3.1.191"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Bacterial outer membrane biogenesis; LPS lipid A biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the transferase hexapeptide repeat family. LpxD subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.191" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316260.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SMZ4"/>
    <dbReference type="SMR" id="Q3SMZ4"/>
    <dbReference type="STRING" id="323098.Nwi_3100"/>
    <dbReference type="EnsemblBacteria" id="ABA06347">
        <property type="protein sequence ID" value="ABA06347"/>
        <property type="gene ID" value="Nwi_3100"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3100"/>
    <dbReference type="eggNOG" id="COG1044">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049865_0_2_5"/>
    <dbReference type="OMA" id="YKIPHAG"/>
    <dbReference type="OrthoDB" id="1602061at2"/>
    <dbReference type="UniPathway" id="UPA00973"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016410">
        <property type="term" value="F:N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009245">
        <property type="term" value="P:lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03352">
        <property type="entry name" value="LbH_LpxD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00523">
        <property type="entry name" value="LpxD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007691">
        <property type="entry name" value="LpxD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020573">
        <property type="entry name" value="UDP_GlcNAc_AcTrfase_non-rep"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43378">
        <property type="entry name" value="PTHR43378"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14602">
        <property type="entry name" value="Hexapep_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04613">
        <property type="entry name" value="LpxD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01853">
        <property type="entry name" value="lipid_A_lpxD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0441">Lipid A biosynthesis</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000264401" description="UDP-3-O-acylglucosamine N-acyltransferase 2">
        <location>
            <begin position="1"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00523"/>
        </source>
    </evidence>
    <sequence length="341" mass="35050" checksum="6BA79AACF0D7CA50" modified="2005-10-11" version="1">MSGETFFLSNHSVAASQIAALAGLSFVTEDFAISAAGALATAGIGEICYMDDTKYIEELKVTRASACLISPRFKELVPGGTFSFVTPHPYAIYAQVLALLYPEATIPASNFGTNGISSKANIHASAIVGHGVTIDPGASVGPNARIGGFTCIGSNAVIGPSVRIGRNCYIGANVTVAYAVVGDRVIIHPGTSIGQDGFGFTFLGGKWVKVPQVGGVIIQDDVEVGANTTIDRGSMRATVIGEGTKLDNLVQVAHNVTIGAHCVIAAQVGIAGSTTIGDFVAIGGHAGIAPHLTIGEKAQIGGASGVMCDIPAGERWVGLPARPSRAFFRQFAALKRLAKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN17</accession>
    <name>Q3SN17_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">50S ribosomal protein L34</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpmH</name>
        <name evidence="6" type="ordered locus">Nwi_3076</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the bacterial ribosomal protein bL34 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3076"/>
    <dbReference type="EnsemblBacteria" id="ABA06324">
        <property type="protein sequence ID" value="ABA06324"/>
        <property type="gene ID" value="Nwi_3076"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3076"/>
    <dbReference type="eggNOG" id="COG0230">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_193756_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00391">
        <property type="entry name" value="Ribosomal_L34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000271">
        <property type="entry name" value="Ribosomal_L34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020939">
        <property type="entry name" value="Ribosomal_L34_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14503">
        <property type="entry name" value="PTHR14503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00468">
        <property type="entry name" value="Ribosomal_L34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01030">
        <property type="entry name" value="rpmH_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00784">
        <property type="entry name" value="RIBOSOMAL_L34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA06324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="83" mass="9376" checksum="5BD7BD0DB5C09145" modified="2005-10-11" version="1">MTARFRPGRLVRAEKRLGSGGLAGLNLNIRRRARSAETPVKRTYQPSKLVRKRRHGFRARLATTGGRKVLAARRARGRKRLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN88</accession>
    <name>Q3SN88_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_3003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316175.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06253">
        <property type="protein sequence ID" value="ABA06253"/>
        <property type="gene ID" value="Nwi_3003"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3003"/>
    <dbReference type="HOGENOM" id="CLU_2369961_0_0_5"/>
    <dbReference type="OMA" id="DNCIRDL"/>
    <dbReference type="OrthoDB" id="2137247at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="95" mass="10635" checksum="34795A99D692FB2F" modified="2005-10-11" version="1">MDDKQILQNATRSAAQAGMITLVFENFTAQLIRYVLSGYLLDDTSLMTLRDNCIRDLKNSTITGLPLDDEAEIFRQAVENAEKLLDAAITRGRDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNE8</accession>
    <name>Q3SNE8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2943</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2943"/>
    <dbReference type="EnsemblBacteria" id="ABA06193">
        <property type="protein sequence ID" value="ABA06193"/>
        <property type="gene ID" value="Nwi_2943"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2943"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155201_0_0_5"/>
    <dbReference type="OrthoDB" id="2044781at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021880">
        <property type="entry name" value="DUF3489"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11994">
        <property type="entry name" value="DUF3489"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="136" mass="14534" checksum="C8E7C6809EBC4272" modified="2005-10-11" version="1">MAKTQSRKAAPRSAMATKRTKSAHPSLTRTAKAAPVKKIISTPARSLDRPVRHRSGSKQALVIEMLMKPEGTTIGDIMKATDWQQHSVRGFFAGVIRKKLKLTLISKAAEAGRIYKVSGDTASTASTGSTSNNTAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNH8</accession>
    <name>Q3SNH8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two component transcriptional regulator, winged helix family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2913</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316093.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2913"/>
    <dbReference type="EnsemblBacteria" id="ABA06163">
        <property type="protein sequence ID" value="ABA06163"/>
        <property type="gene ID" value="Nwi_2913"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2913"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_1_5"/>
    <dbReference type="OMA" id="AVHIRHI"/>
    <dbReference type="OrthoDB" id="1020672at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="2"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="4">
        <location>
            <begin position="124"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="124"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA06163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="224" mass="24505" checksum="ECF00CFDA2A4A258" modified="2005-10-11" version="1">MRVLIVEDSEELADLLAKGLRAAGYQIDLLTNLADAASALGTTRYAALILDLGLPDGDGLSILRELRHRKDPLPILVLTARGGVEDRVSGLRSGADDYLVKPFSFDELVARLEALLRRPGQLLGSSLRLANVTFDSEGRQAFVDMKPQNLTAREIAVLELLLRRKGNVVSKKLLEDQIFGLTGDVASNAVEVYVHRLRKQLLDIGAKVKISTIRGVGYVMMESD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNJ2</accession>
    <name>Q3SNJ2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Integrase, catalytic region</fullName>
            <ecNumber evidence="2">2.7.7.49</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2899</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316079.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2899"/>
    <dbReference type="EnsemblBacteria" id="ABA06149">
        <property type="protein sequence ID" value="ABA06149"/>
        <property type="gene ID" value="Nwi_2899"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2899"/>
    <dbReference type="eggNOG" id="COG2801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027402_15_1_5"/>
    <dbReference type="OMA" id="FHIDIDE"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13683">
        <property type="entry name" value="rve_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="127"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="327" mass="37586" checksum="4A71E750A5D12EAB" modified="2005-10-11" version="1">MGQVLHGSATTTEAIRRAIQNSEESLRALSKRYGINQKTVAKWKKRTSVADLPTGPKDPRSTVLSSEEEAIIVAFRKHTLLPLDDCLYALQPTIPHLSRSSLHRCLHRHGISRLPEVEGDKPIRKTFRSYPIGYFHIDIAEVQTAEGKLRLFVAIDRTSKFAYAELHQEAGKMVAAQFLRNLIVAVPYAIHTVLTDNGIQFTNHARHKYAFHHIFDRVCDENGIEHRLTRINHPWTNGQVERMNRTIKDATVKRFHYDNHDQLRRHLQDFIKAYNFGRRLKTLKGLTPYEFICKRWTSEPDRFIIDPIHQMPGLNISSIPGESNASS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNK1</accession>
    <name>METE_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cobalamin-independent methionine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Methionine synthase, vitamin-B12 independent isozyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">metE</name>
        <name type="ordered locus">Nwi_2890</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = L-methionine + tetrahydropteroyltri-L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:21196"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58140"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="ChEBI" id="CHEBI:58207"/>
            <dbReference type="EC" id="2.1.1.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the vitamin-B12 independent methionine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.14" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SNK1"/>
    <dbReference type="SMR" id="Q3SNK1"/>
    <dbReference type="STRING" id="323098.Nwi_2890"/>
    <dbReference type="EnsemblBacteria" id="ABA06140">
        <property type="protein sequence ID" value="ABA06140"/>
        <property type="gene ID" value="Nwi_2890"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2890"/>
    <dbReference type="eggNOG" id="COG0620">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013175_0_0_5"/>
    <dbReference type="OMA" id="KVMKGML"/>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00082"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003871">
        <property type="term" value="F:5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03311">
        <property type="entry name" value="CIMS_C_terminal_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00172">
        <property type="entry name" value="Meth_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013215">
        <property type="entry name" value="Cbl-indep_Met_Synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006276">
        <property type="entry name" value="Cobalamin-indep_Met_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002629">
        <property type="entry name" value="Met_Synth_C/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08267">
        <property type="entry name" value="Meth_synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01717">
        <property type="entry name" value="Meth_synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000382">
        <property type="entry name" value="MeTrfase_B12_ind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="SSF51726"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01371">
        <property type="entry name" value="met_syn_B12ind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0486">Methionine biosynthesis</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_1000017260" description="5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase">
        <location>
            <begin position="1"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="714"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="13"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>5-methyltetrahydropteroyltri-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58207"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>5-methyltetrahydropteroyltri-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58207"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="451"/>
            <end position="453"/>
        </location>
        <ligand>
            <name>L-homocysteine</name>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="451"/>
            <end position="453"/>
        </location>
        <ligand>
            <name>L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="504"/>
        </location>
        <ligand>
            <name>L-homocysteine</name>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="504"/>
        </location>
        <ligand>
            <name>L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="535"/>
            <end position="536"/>
        </location>
        <ligand>
            <name>5-methyltetrahydropteroyltri-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58207"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="581"/>
        </location>
        <ligand>
            <name>5-methyltetrahydropteroyltri-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58207"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="619"/>
        </location>
        <ligand>
            <name>L-homocysteine</name>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="619"/>
        </location>
        <ligand>
            <name>L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="625"/>
        </location>
        <ligand>
            <name>5-methyltetrahydropteroyltri-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58207"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="661"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="663"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="685"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="746"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00172"/>
        </source>
    </evidence>
    <sequence length="771" mass="85031" checksum="6EA92CAB17352A84" modified="2005-10-11" version="1">MATLGTPRIGPRRELKTALESYWSGKSDESALLEAAAGLRAANWARQKSLGVTVIPSNDFTFYDHVLDTSVMVGAIPDIYGWKSGPVPLATYFAMARGAQGGNDDAACAHGHQGDGHAHGAPAQEMTKWFDTNYHYMVPEFTSGQRFQLSSLKAIDEYREAKALGYQTRPVLLGPVTYLKLGKSKDANLDPLSLLPDLLPVYIDVLKRLAAEGAEWVQIDEPCLVLDLDDATRKVLRAAYEQLADALPKLKVMLTTYFGGLGDNLDAALSLPVTGLHIDLARAPDQLEAVAAKAPHDLILSLGVIDGRNIWRANLPALLDRLEPVVSERGADRVQIAPSCSLLHVPIDVSLETDLDAELKSWLAFSVQKMGELATLGEALAKGRASVADALKASADAAAARKASPKVHDAKVEGRIAAVTSDMARRKSAFAERAKLQRERFGLPPFPTTTIGSFPQTAEVRKARSAHAKGTLSDTDYEKFLQEETARTIRWQEDIGLDVLVHGEFERNDMVQYFGEQLSGFAFTRHGWVQSYGSRCVRPPVLFGDVSRPRPMTVGWWKYAQSLTAKPMKGMLTGPVTILNWSFVRDDVPRSLACRQIALAIRDEVSDLEQAGATMIQIDEAALREGLPLRRSEWKTYLDWAVECFRLCASGVADATQIHTHMCYSEFNDIIDAIASMDADVISIETSRSKMELLDAFKTYKYPNEIGPGVYDIHSPRVPAVDEMTGLLKLARERLSDRQIWINPDCGLKTRKWEEVRPALVNMVEAAKLMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNQ5</accession>
    <name>Q3SNQ5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2834</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316026.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2834"/>
    <dbReference type="EnsemblBacteria" id="ABA06086">
        <property type="protein sequence ID" value="ABA06086"/>
        <property type="gene ID" value="Nwi_2834"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2834"/>
    <dbReference type="eggNOG" id="COG1961">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132664_0_0_5"/>
    <dbReference type="OMA" id="EAGEFNT"/>
    <dbReference type="OrthoDB" id="1362093at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="127" mass="14655" checksum="8B0D47F4054EFDAB" modified="2005-10-11" version="1">MIKTDDTIQLFVPLKVRKQNGRPKIMPPANYLPSEDRTQDPHILRAIGRAWGWRRRMEAGEFNTVTDLAKSVGLAERHVSRQLRLAYLAPGVLKRLVYRRDVPVVTLLKLTDVAALPWDEQPERVFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNT6</accession>
    <name>Q3SNT6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">OmpA/MotB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2802</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2802"/>
    <dbReference type="EnsemblBacteria" id="ABA06055">
        <property type="protein sequence ID" value="ABA06055"/>
        <property type="gene ID" value="Nwi_2802"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2802"/>
    <dbReference type="eggNOG" id="COG2885">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021407_0_1_5"/>
    <dbReference type="OMA" id="FFIEGHT"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07185">
        <property type="entry name" value="OmpA_C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006664">
        <property type="entry name" value="OMP_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006665">
        <property type="entry name" value="OmpA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036737">
        <property type="entry name" value="OmpA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00691">
        <property type="entry name" value="OmpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01021">
        <property type="entry name" value="OMPADOMAIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103088">
        <property type="entry name" value="SSF103088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51123">
        <property type="entry name" value="OMPA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OmpA-like" evidence="4">
        <location>
            <begin position="529"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="65"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="112"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="264"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="283"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="310"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA06055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="663" mass="69827" checksum="293D6D3356CDA2B6" modified="2005-10-11" version="1">MSDPLARKRITATLQPYRYRLIGSPLTDGRLVLLPKSLLEEIAMTHFRLLLLATSALTLSSLTVTPSQAQSGPVQFAQATPSGENGDKDKRKGPPKAAPDRPAASQPAAAPPHARPKAPKPPTAAPPRPAIPPAAPPSRPTASPQRATPPAAPETPRAAPSPQPATPPAAPKPPAPSAVAPPSPPKGTPPAAMKPAPATSAPIAPPQATRPTTPPAGAEPNAMRRDEQGRGRFERNRGEPGGPGERQKGAAPAPKTTPAAGSPSDAPKPAPANPPVAAPAASPSPNPNGTKAGSEPPTPASRTARPAGAPRAPQAPPTVTAPLPPPPPVADKVVAPGAAPQGPRRIEDFRSRRQETQQGGRTIITEPGRVIVRDPSGQSFIRHNESDRFRRGARDIRQERVGNDTRNIVVRQDGTQIITVNDRDGRMLQRIRRDPRGQEIVIIDNRHPGHGGRGGALAAGVAAGIAAGAIGSYFVSLPPPQIGIPPDRYIVEAEGAPPEVIYDTLMAPPVESIQRPYTLDEVRYSPMVRARMPSIDLDTINFETGSWDISDDQAAKLQVIADGINRVVARNPGEVFLIEGHTDAVGNDVDNLSLSDRRAQSAAELLTQQFQVPPENLVTQGYGEQNLKIQTDGPSRENRRVTVRRITPLLNGGQTAGYQQPPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNX7</accession>
    <name>Q3SNX7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Adenosine 5'-phosphosulfate reductase</fullName>
            <shortName evidence="3">APS reductase</shortName>
            <ecNumber evidence="3">1.8.4.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">5'-adenylylsulfate reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Thioredoxin-dependent 5'-adenylylsulfate reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cysH</name>
        <name evidence="5" type="ordered locus">Nwi_2761</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the formation of sulfite from adenosine 5'-phosphosulfate (APS) using thioredoxin as an electron donor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>[thioredoxin]-disulfide + AMP + 2 H(+) + sulfite = [thioredoxin]-dithiol + adenosine 5'-phosphosulfate</text>
            <dbReference type="Rhea" id="RHEA:21976"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17359"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="1.8.4.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="3">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the PAPS reductase family. CysH subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.10" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315959.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2761"/>
    <dbReference type="EnsemblBacteria" id="ABA06014">
        <property type="protein sequence ID" value="ABA06014"/>
        <property type="gene ID" value="Nwi_2761"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2761"/>
    <dbReference type="eggNOG" id="COG0175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044089_2_1_5"/>
    <dbReference type="OMA" id="PIARWTQ"/>
    <dbReference type="OrthoDB" id="674332at2"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004604">
        <property type="term" value="F:phosphoadenylyl-sulfate reductase (thioredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019379">
        <property type="term" value="P:sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01713">
        <property type="entry name" value="PAPS_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00063">
        <property type="entry name" value="CysH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004511">
        <property type="entry name" value="PAPS/APS_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002500">
        <property type="entry name" value="PAPS_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01507">
        <property type="entry name" value="PAPS_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000857">
        <property type="entry name" value="PAPS_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAPS_reduct" evidence="4">
        <location>
            <begin position="58"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; cysteine thiosulfonate intermediate" evidence="3">
        <location>
            <position position="246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA06014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="254" mass="27372" checksum="ED8C7842D3E677D7" modified="2005-10-11" version="1">MTSPLEENSVDPGRAAQSAAAAANSAWPAAAALNESLRDASPAEVISAALRAVGRDRLALVSSFGTESAALLKVMADVDPAIPVIFLDTGWLFEETLTYRDTLIAALGLKDVRSIPPSEDALKQEDPDRELWFSDPDSCCRIRKVEPLARALQPFAAWINGRKRFQGGLRADLPMVEDDGARLKFNPFANASREDIEAIYASAKLPPHPLVASGFRSVGCMPCTSRGQAGEDARAGRWRGRAKTECGIHTMKIP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP13</accession>
    <name>RUVA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Holliday junction ATP-dependent DNA helicase RuvA</fullName>
            <ecNumber evidence="1">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ruvA</name>
        <name type="ordered locus">Nwi_2725</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a complex with RuvB.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RuvA family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315924.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SP13"/>
    <dbReference type="SMR" id="Q3SP13"/>
    <dbReference type="STRING" id="323098.Nwi_2725"/>
    <dbReference type="EnsemblBacteria" id="ABA05978">
        <property type="protein sequence ID" value="ABA05978"/>
        <property type="gene ID" value="Nwi_2725"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2725"/>
    <dbReference type="eggNOG" id="COG0632">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087936_3_0_5"/>
    <dbReference type="OMA" id="VGMAVQC"/>
    <dbReference type="OrthoDB" id="1370858at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009379">
        <property type="term" value="C:Holliday junction helicase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14332">
        <property type="entry name" value="UBA_RuvA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00031">
        <property type="entry name" value="DNA_helic_RuvA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013849">
        <property type="entry name" value="DNA_helicase_Holl-junc_RuvA_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000085">
        <property type="entry name" value="RuvA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011114">
        <property type="entry name" value="RuvA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036267">
        <property type="entry name" value="RuvA_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07499">
        <property type="entry name" value="RuvA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01330">
        <property type="entry name" value="RuvA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46929">
        <property type="entry name" value="SSF46929"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00084">
        <property type="entry name" value="ruvA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0742">SOS response</keyword>
    <feature type="chain" id="PRO_0000224885" description="Holliday junction ATP-dependent DNA helicase RuvA">
        <location>
            <begin position="1"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00031"/>
        </source>
    </evidence>
    <sequence length="205" mass="21709" checksum="C8FBA1D6C8490BA0" modified="2005-10-11" version="1">MIGKLKGVIDSYGEDYVILDVGGVGYQVHCASRTLQALPSPGQAAVLSIETYVREDQIKLFGFRTDHEREWFRLLQTVQGVGARVALAVLGTLSPADLASAIALRDKASVARTPGIGPKVAERIVTELKDKAPSFANVDPTVVHLAGDLDDQRAPRPVRDAISALVNLGYGQPQATAAIAAASRGAGENAETAQLIRLGLKELSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP16</accession>
    <name>Q3SP16_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transcriptional regulator, TetR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2722</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315921.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2722"/>
    <dbReference type="EnsemblBacteria" id="ABA05975">
        <property type="protein sequence ID" value="ABA05975"/>
        <property type="gene ID" value="Nwi_2722"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2722"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_40_0_5"/>
    <dbReference type="OMA" id="HIWSMSH"/>
    <dbReference type="OrthoDB" id="1570162at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025996">
        <property type="entry name" value="TetR_C_WHG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13305">
        <property type="entry name" value="TetR_C_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="6">
        <location>
            <begin position="17"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="4">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="211"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="237" mass="25906" checksum="F185A96490EB5F62" modified="2005-10-11" version="1">MSWRKDHRRPERGYHHGNLKEALLQAALGLIAEKGAAGFTFADAARSAGVSPAAPYRHFRDRDELLASIAQRGFEQFEAVLTRAWDDGRPDTVTAFERVGRAYLAFARDEPAYYSAMFESGLPTDVNPMLSAASERAFSVIRAAAERLAALTPPGAARPPALMMALHIWSMSHGIASLFGRGDAARRKMPMSAEDLLEAGVLIYLRGLGFPTDRRSPEDSNPPAGRPRGPWDQKSAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP24</accession>
    <name>Q3SP24_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidoglycan-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pal</name>
        <name evidence="12" type="ordered locus">Nwi_2714</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the Tol-Pal system, which plays a role in outer membrane invagination during cell division and is important for maintaining outer membrane integrity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">The Tol-Pal system is composed of five core proteins: the inner membrane proteins TolA, TolQ and TolR, the periplasmic protein TolB and the outer membrane protein Pal. They form a network linking the inner and outer membranes and the peptidoglycan layer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Pal lipoprotein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315913.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2714"/>
    <dbReference type="EnsemblBacteria" id="ABA05967">
        <property type="protein sequence ID" value="ABA05967"/>
        <property type="gene ID" value="Nwi_2714"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2714"/>
    <dbReference type="eggNOG" id="COG2885">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016890_9_2_5"/>
    <dbReference type="OMA" id="MNQGEET"/>
    <dbReference type="OrthoDB" id="1544679at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07185">
        <property type="entry name" value="OmpA_C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02204">
        <property type="entry name" value="Pal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006664">
        <property type="entry name" value="OMP_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006665">
        <property type="entry name" value="OmpA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006690">
        <property type="entry name" value="OMPA-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036737">
        <property type="entry name" value="OmpA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039001">
        <property type="entry name" value="Pal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014169">
        <property type="entry name" value="Pal_lipo_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00691">
        <property type="entry name" value="OmpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01021">
        <property type="entry name" value="OMPADOMAIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103088">
        <property type="entry name" value="SSF103088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02802">
        <property type="entry name" value="Pal_lipo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01068">
        <property type="entry name" value="OMPA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51123">
        <property type="entry name" value="OMPA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1 8" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0564">Palmitate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229138" description="Peptidoglycan-associated protein" evidence="10">
        <location>
            <begin position="28"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="OmpA-like" evidence="11">
        <location>
            <begin position="50"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA05967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="167" mass="18214" checksum="F2E2AA2B633B49C9" modified="2005-10-11" version="1">MLNQKRFLRGLKLAAVLAAALSMGACANKNPMDGSGGRGGMAGMAAPGSQQDFVVNVGDRVFFESDQTSLSPQAITTLEKQAHWLQTYHRYSFTIEGHADERGTREYNIALGARRAQSVRSFLASRGIDPSRMRTISYGKERPVAVCNDISCWSQNRRAVTVLNASS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-09-11" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP50</accession>
    <name>KHSE_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Homoserine kinase</fullName>
            <shortName evidence="1">HK</shortName>
            <shortName evidence="1">HSK</shortName>
            <ecNumber evidence="1">2.7.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thrB</name>
        <name type="ordered locus">Nwi_2688</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-homoserine = ADP + H(+) + O-phospho-L-homoserine</text>
            <dbReference type="Rhea" id="RHEA:13985"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57590"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.39"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudomonas-type ThrB family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.39" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315887.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SP50"/>
    <dbReference type="SMR" id="Q3SP50"/>
    <dbReference type="STRING" id="323098.Nwi_2688"/>
    <dbReference type="EnsemblBacteria" id="ABA05941">
        <property type="protein sequence ID" value="ABA05941"/>
        <property type="gene ID" value="Nwi_2688"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2688"/>
    <dbReference type="eggNOG" id="COG2334">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053300_1_0_5"/>
    <dbReference type="OMA" id="DPTHFER"/>
    <dbReference type="OrthoDB" id="1003984at2"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00064"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004413">
        <property type="term" value="F:homoserine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05153">
        <property type="entry name" value="HomoserineK_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00301">
        <property type="entry name" value="Homoser_kinase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005280">
        <property type="entry name" value="Homoserine_kinase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00938">
        <property type="entry name" value="thrB_alt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0791">Threonine biosynthesis</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000300796" description="Homoserine kinase">
        <location>
            <begin position="1"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00301"/>
        </source>
    </evidence>
    <sequence length="326" mass="35859" checksum="07DF1E2435B0FE5E" modified="2005-10-11" version="1">MAVYTDVAAEELADFLKAYDIGELLSYKGIAEGVENSNYLLHTTSGSFFLTLYEKRVAIDDLPFFLGLMGHLATHGIVCPQPVRTRNGETLGSLAGRPAAIIDFLEGVWPRKPNVAHCAAVGAALAKLHLAGRDFSMRRANALSVWSWRPLFEQAGACADTVQPGLHDFLKAELDHLEARWPQELPGGVIHADLFPDNVLFLGDRLSGLIDFYFACHDFFAYDVAICLNAWCFEPDHAFNVTKARALLGAYHRERALSEAEQAALPLLARGAALRFLLTRLVDFLNVPAGALVKPKDPLEYVRKLRFQQRVGGVRDYGVAILGPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP55</accession>
    <name>Q3SP55_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Universal stress protein, UspA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2683</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315882.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05936">
        <property type="protein sequence ID" value="ABA05936"/>
        <property type="gene ID" value="Nwi_2683"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2683"/>
    <dbReference type="eggNOG" id="COG0589">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_11_0_5"/>
    <dbReference type="OMA" id="VSHKVSQ"/>
    <dbReference type="OrthoDB" id="2040681at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Usp" evidence="2">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="148" mass="15413" checksum="EF6B321E30C92B5A" modified="2005-10-11" version="1">MHSHILIPTDGSELAQKGVDYGLSLADGYGGKVTIITVTEQSPVPSIGESEWGKVGMDPDRLEDADTRHAKAILSAAKAAAEKIGLTVETLHTQNVSPAAAIVDVAKKNGCSLIVMASHRRRGIKSVLLGSQTAEVLATAPIPVLIVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP84</accession>
    <name>Q3SP84_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Multisubunit potassium/proton antiporter, PhaA subunit / multisubunit potassium/proton antiporter, PhaB subunit</fullName>
            <ecNumber evidence="15">1.6.99.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">Nwi_2654</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.6.99.5" evidence="15"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315857.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2654"/>
    <dbReference type="EnsemblBacteria" id="ABA05907">
        <property type="protein sequence ID" value="ABA05907"/>
        <property type="gene ID" value="Nwi_2654"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2654"/>
    <dbReference type="eggNOG" id="COG1009">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2111">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007100_2_0_5"/>
    <dbReference type="OMA" id="AFILQYM"/>
    <dbReference type="OrthoDB" id="1274678at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005451">
        <property type="term" value="F:monovalent cation:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098662">
        <property type="term" value="P:inorganic cation transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025383">
        <property type="entry name" value="MbhD-like_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007182">
        <property type="entry name" value="MnhB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005663">
        <property type="entry name" value="MrpA/MnhA1/PhaAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_mem"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001516">
        <property type="entry name" value="Proton_antipo_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13244">
        <property type="entry name" value="DUF4040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04039">
        <property type="entry name" value="MnhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00662">
        <property type="entry name" value="Proton_antipo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00940">
        <property type="entry name" value="2a6301s01"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="139"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="168"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="211"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="248"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="276"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="304"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="373"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="416"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="457"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="485"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="512"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="581"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="611"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="636"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="662"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="708"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="764"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="826"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="850"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="882"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="925"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="domain" description="Proton_antipo_N" evidence="12">
        <location>
            <begin position="69"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Proton_antipo_M" evidence="11">
        <location>
            <begin position="132"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4040" evidence="14">
        <location>
            <begin position="623"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="domain" description="MnhB" evidence="13">
        <location>
            <begin position="824"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="793"/>
            <end position="812"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABA05907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="980" mass="106239" checksum="67F1FA44AA2A19A0" modified="2005-10-11" version="1">MTMPYETILIVVLALPFAGSCVAAVLPSNARNAEAWLAGAVSLACVVLLAVSYPQIDDGGVIRRTVEWMPELGLDFYLRMDGFAWVLAALIVTIGFLVVLYARYYMSPQDPVPRFFSFLLAFMGAMLGIVLSGNLIQLVFFWELTSLFSFLLIGYWHQTAQARDGARMALIVTSVGGFCMFAGILILGNIVGSYDLDQVLASGDKIRAHSLYTPTLVLILLGALTKSAQFPFHFWLPQAMAAPTPVSAYLHSATMVKGGVFLLVRLWPALGGTDEWLWLVGSAGLISFMLGAFIALFQQDMKGLLAYSTISHLGLITLLIGLDSPIAQVAAIFHLMNHATFKASLFMAAGIIDHETGTRDLRRLSGLRRFMPITATLAMVAAAAMAGVPLLNGFLSKEMFFAETIETHTNSLLDEALPYIVTAASMFTVAYSLRFIAEVFFGPPPKELTRTPHEPPFLMRFPVGLLVLACLLVGIVPSLTIGPLLLTALHAVLGDIIPIYSLAIWHGFTPEFVMSLVAITGGIAIYLMLRSYLLIAEGPPLLRYIQGQRIFDRVLVAISWQLARRLEELLGTRRLQPQLRLVFCAALLAGIAPMFAHGIDIWGAPRTSFDVTFALIWAVGGTCALAAAYQGKFHRFAALIFMGGAGLVTCVSFVWLAAPDLALTQLAVETVTTVLLLLGLRWLPKRIEKFPAADVPERDLSSRRYRDLGIAIASGGGLAALAYAVMTRAAPDSIARFFVENAYTLGGGTNIVNVILVDFRAFDTFGEITVLGIVALTVYALLRRFRPAAESIPVPEQQSSQDLYDEAQPSREKGDTLRSAMAVPRLMMALLFPVICTTAAFLLLRGHDLPGGGFVAGITMAVAFILQYIARGTTWTEDHLRILPVHWMGAGLLMTAGVGVGAWWFERPFLSSSFSYHEIPWIGPVPVASALLFDLGVFALVVGATVLMLIAIAHQTVRSHRTPSSRASSAAAASATAEVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP87</accession>
    <name>Q3SP87_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Multicopper oxidase3</fullName>
            <ecNumber evidence="4">1.10.3.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2651</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.10.3.3" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315854.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2651"/>
    <dbReference type="EnsemblBacteria" id="ABA05904">
        <property type="protein sequence ID" value="ABA05904"/>
        <property type="gene ID" value="Nwi_2651"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2651"/>
    <dbReference type="eggNOG" id="COG2132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064932_1_0_5"/>
    <dbReference type="OMA" id="MFHPHQD"/>
    <dbReference type="OrthoDB" id="971126at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008447">
        <property type="term" value="F:L-ascorbate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="PTHR11709"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="SSF49503"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229086" evidence="1">
        <location>
            <begin position="39"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="3">
        <location>
            <begin position="56"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="2">
        <location>
            <begin position="203"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="358" mass="40445" checksum="515B5609167D243D" modified="2005-10-11" version="1">MHVMPMIVQCRALRARLAKVIFAASAALCAVSPGAALAETRQFSMIIEDTILKLVDKQTFHVFSFNGQSPGPLFHVKEGDDVEIEVENQTTLPHTIHWHGLYQRGTWKHDGVPDVTQPAIPPGETFTYRFKAEPSGTMWYHCHVNVNEHVAMRGMWGPFIIDPKDPPPVEKEVTKDYIMMFSSWTSAWAKKPGYGGVPGDVPDFFTINGKAYPDTQPLRIKEGDVVRIRLIGAGDELHSIHIHGHLFEVYAKDGRPLANPYLADTILFGPGERYDLILRADNPGIWMVHDHIDKHTTNGSSPMGGMMTTIEYEEIPVKEQSFYKWKNKEFVPDFYYEESMKKPLDMHESPAFKGDPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPB7</accession>
    <name>Q3SPB7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Oxygen-dependent coproporphyrinogen-III oxidase</fullName>
            <shortName evidence="5">CPO</shortName>
            <shortName evidence="5">Coprogen oxidase</shortName>
            <shortName evidence="5">Coproporphyrinogenase</shortName>
            <ecNumber evidence="5">1.3.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">hemF</name>
        <name evidence="6" type="ordered locus">Nwi_2621</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the heme biosynthesis. Catalyzes the aerobic oxidative decarboxylation of propionate groups of rings A and B of coproporphyrinogen-III to yield the vinyl groups in protoporphyrinogen-IX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>coproporphyrinogen III + 2 H(+) + O2 = 2 CO2 + 2 H2O + protoporphyrinogen IX</text>
            <dbReference type="Rhea" id="RHEA:18257"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57307"/>
            <dbReference type="ChEBI" id="CHEBI:57309"/>
            <dbReference type="EC" id="1.3.3.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the aerobic coproporphyrinogen-III oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.3.3" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315824.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2621"/>
    <dbReference type="EnsemblBacteria" id="ABA05874">
        <property type="protein sequence ID" value="ABA05874"/>
        <property type="gene ID" value="Nwi_2621"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2621"/>
    <dbReference type="eggNOG" id="COG0408">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026169_0_1_5"/>
    <dbReference type="OMA" id="HDKGTLF"/>
    <dbReference type="OrthoDB" id="1086432at2"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00322"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004109">
        <property type="term" value="F:coproporphyrinogen oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1500.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00333">
        <property type="entry name" value="Coprogen_oxidas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001260">
        <property type="entry name" value="Coprogen_oxidase_aer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036406">
        <property type="entry name" value="Coprogen_oxidase_aer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018375">
        <property type="entry name" value="Coprogen_oxidase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10755">
        <property type="entry name" value="PTHR10755"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01218">
        <property type="entry name" value="Coprogen_oxidas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000166">
        <property type="entry name" value="Coproporphyri_ox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00073">
        <property type="entry name" value="COPRGNOXDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102886">
        <property type="entry name" value="SSF102886"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01021">
        <property type="entry name" value="COPROGEN_OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 5" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="133"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Important for dimerization" evidence="5">
        <location>
            <begin position="265"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="283"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for dimerization" evidence="5">
        <location>
            <position position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="300" mass="33871" checksum="40F83A5B2C281026" modified="2005-10-11" version="1">MEATRLEDRTLEDRKARARSWFEQLRDDICSAFEKLEDDAPASLYPGSAGRFVRTPWERTDHTGKPGGGGVMSMMHGRLFEKVGVHCSTVHGEFAPEFRAQIPGAADDPNFWASGISLIAHMRNPHIPAVHMNTRFVVTTKAWFGGGGDLTPVLMRRRTQEDPDSVAFHAAMKAACDAHAAIAPYDKYKTWCDEYFHLPHRKEARGIGGIFYDWHDSGDWNADLAFTQDVGRALLKVYPELVRRNFSTPWTAADREEQLIRRGRYAEFNLLYDRGTIFGLKTGGNVESILSSMPPEVKWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPP4</accession>
    <name>Q3SPP4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2494</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2494"/>
    <dbReference type="EnsemblBacteria" id="ABA05747">
        <property type="protein sequence ID" value="ABA05747"/>
        <property type="gene ID" value="Nwi_2494"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2494"/>
    <dbReference type="eggNOG" id="ENOG50314GF">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_173275_2_0_5"/>
    <dbReference type="OMA" id="CWCAEET"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="78" mass="8525" checksum="2266CFA91D635669" modified="2005-10-11" version="1">MVAHRSTWPENAPMTRPIGQQAVRRLICSGCGTEFNCDPSGNCWCAEEPGRLPMPTEGGDCLCRDCLRKAVSRSSPKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPW4</accession>
    <name>Q3SPW4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AraR_C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2423</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315635.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2423"/>
    <dbReference type="EnsemblBacteria" id="ABA05677">
        <property type="protein sequence ID" value="ABA05677"/>
        <property type="gene ID" value="Nwi_2423"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2423"/>
    <dbReference type="eggNOG" id="COG4111">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067794_0_0_5"/>
    <dbReference type="OMA" id="GHRTLQS"/>
    <dbReference type="OrthoDB" id="1846332at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045830">
        <property type="entry name" value="AraR_wHTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011213">
        <property type="entry name" value="NMN_biosyn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19368">
        <property type="entry name" value="AraR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019423">
        <property type="entry name" value="NMN_biosyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AraR_C" evidence="1">
        <location>
            <begin position="241"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="325" mass="36332" checksum="31BCF09F0D60253D" modified="2005-10-11" version="1">MSDPLTKSTASVHSLSPASPQAIAADLIALLVAVTAGDPRVLTIQDQNALPSGPFELAHRSLQSGLRAWVERQTGLPLGYVEQLYTFADRDRTGAADQRIISISYLGLTREQAQSSQYEASWRSWYDYFPWEDHRLGIPTMEKTLRSGLAAWIAAAPDRATRSHRRQRAARLFGLEDHLWNEELVLQRYELLYEARLIPEALRSEGPSAGVAAFVPGDPMILDHRRILATGIARLRAKIKYRPVVFELMPDTFTLLQLQRCVEALAGKLVHKPNFRRLIEQQELVEDTGETSQETGGRPAKLFRFRRAVLAERAIAGTKLPVSGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ22</accession>
    <name>Q3SQ22_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FAD dependent oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2365</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315581.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2365"/>
    <dbReference type="EnsemblBacteria" id="ABA05619">
        <property type="protein sequence ID" value="ABA05619"/>
        <property type="gene ID" value="Nwi_2365"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2365"/>
    <dbReference type="eggNOG" id="COG1233">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019327_0_1_5"/>
    <dbReference type="OMA" id="GMQGAWG"/>
    <dbReference type="OrthoDB" id="453021at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amino_oxidase" evidence="1">
        <location>
            <begin position="15"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="535" mass="57427" checksum="D70AA02FF63B8EA6" modified="2005-10-11" version="1">MSDTDVVIIGAGHNGLTCAAYLAMAGLRVRVVERRKVVGGAAVTEEFHPGFRNSVAAYTVSLLNPKVIADLRLHDHGLRIVERRAQNFLPAPDGRYLLTGEGRTKASAARLSERDAAVLDEFMRELEEIADVVRQFVLRAPPNLGEGFGLKAAGELVNAIGSAGILRGLSLEQQRSLLDLFTRSAGDMLDDRFETDLIKSLFGFDAIVGNYASPYAAGSAYVMLHHAFGEVNGKKGVWGHAIGGMGAITQAMAAAARAHGVEIETDASVREVVVERKRAAGAVLDDGRTIRARYVAANVNPKLLYTRLLPAGSVPPSFLARIRNWRNGSGTFRMNVALSALPSFSALPGAGDHLSAGIIIAPGLDYMDRAWHDARTFGWSREPVVEVLIPSVIDDTLAPSGRHVASLFCQHVAPRLPDGTSWDDHRDEVADLMIATVDRYAPGFAASVIGRQILSPLDLEREFGLLGGDIFHGALTLNQLFSARPMLGYADYRGPLKGLYHCGSGAHPGGGVTGAPGHNAARAILRDHRTLFARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ39</accession>
    <name>Q3SQ39_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein HflC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2348</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">HflC and HflK could regulate a protease.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the band 7/mec-2 family. HflC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315566.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2348"/>
    <dbReference type="MEROPS" id="I87.001"/>
    <dbReference type="EnsemblBacteria" id="ABA05602">
        <property type="protein sequence ID" value="ABA05602"/>
        <property type="gene ID" value="Nwi_2348"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2348"/>
    <dbReference type="eggNOG" id="COG0330">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059167_1_0_5"/>
    <dbReference type="OMA" id="DFFAFYR"/>
    <dbReference type="OrthoDB" id="1714999at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052547">
        <property type="term" value="P:regulation of peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03405">
        <property type="entry name" value="SPFH_HflC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010200">
        <property type="entry name" value="HflC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42911">
        <property type="entry name" value="PTHR42911"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005651">
        <property type="entry name" value="HflC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="SSF117892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PHB" evidence="3">
        <location>
            <begin position="23"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR005651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="298" mass="33288" checksum="1B64246D0481012C" modified="2005-10-11" version="1">MKTGVLGIAALLVGLALLVVGYSSVFTVGQTEQVLLVRLGEPVRVVTEPGLHFKAPFVDSVIEIDKRILDLEQASQEVIASDQKRLVVDAFARYRIKDALRFYQSVGSIQVANIQLTTLLNASLRRVLGEVTFIQVVRDEREMLMARIRDQLDKEASGYGISVVDVRIRRADLPEQNSQAIYQRMQTERQREAAEFRAQGGQKAQEIRAKADREATVIIAEANSAAERIRGQGDGERNRLFAQAYNQDPAFFAFYRSMSAYQNGLKSSDTRFLLKPDSDFFRFFGHIGGRSAASAPKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ41</accession>
    <name>Q3SQ41_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Periplasmic serine endoprotease DegP-like</fullName>
            <ecNumber evidence="5">3.4.21.107</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2346</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>Acts on substrates that are at least partially unfolded. The cleavage site P1 residue is normally between a pair of hydrophobic residues, such as Val-|-Val.</text>
            <dbReference type="EC" id="3.4.21.107"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase S1C family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.107" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315564.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2346"/>
    <dbReference type="EnsemblBacteria" id="ABA05600">
        <property type="protein sequence ID" value="ABA05600"/>
        <property type="gene ID" value="Nwi_2346"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2346"/>
    <dbReference type="eggNOG" id="COG0265">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020120_1_0_5"/>
    <dbReference type="OMA" id="NRSVSMR"/>
    <dbReference type="OrthoDB" id="741829at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011782">
        <property type="entry name" value="Pept_S1C_Do"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001940">
        <property type="entry name" value="Peptidase_S1C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00834">
        <property type="entry name" value="PROTEASES2C"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="SSF50494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02037">
        <property type="entry name" value="degP_htrA_DO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5010600454" description="Periplasmic serine endoprotease DegP-like" evidence="5">
        <location>
            <begin position="35"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="6">
        <location>
            <begin position="272"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="6">
        <location>
            <begin position="383"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="4">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="4">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="4">
        <location>
            <position position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR611782-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="496" mass="52575" checksum="767413338C401247" modified="2005-10-11" version="1">MTRAMQALSLRRRFLLSAFCLGVASAVVSAPALARGPDGIADVAEKVIDAVVNISTTQKVEAKSGEGRRMIPQLPPGSPFEEFFDDFFKNRRGGKSDGLQTHKTNSLGSGFIVDAAGIVVTNNHVIADSDEINVILNDGTKVKAETVGVDKKTDLAVLKFKPPHPLMAVKFGDSDKLRLGEWVIAIGNPFSLGGSVSAGIVSARNRDINNGPYDSYIQTDAAINRGNSGGPLFNLEGEVVGVNTLIISPSGGSIGIGFAVPSKTVAGVVDQLRKFGELRRGWLGVRIQQVTDEIADSLNIKPARGALVAGVEDKGPAKPAGIEPGDVVIAFDGKDIKEPKDLSRMVADTAVGKTVSVVVIRKGKEETKKVTLGRLDDSDKAIPVSIKTKPEDDDKPVTQKALGLDLATLSKDLRARYKIKDSVKGVIITEVEADSDAAEKRLSAGDVIVEVAQEAVNSASDIRKRIDQLKKDGKKAVLLMVSNGAGELRFVALSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ62</accession>
    <name>Q3SQ62_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Antibiotic biosynthesis monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315544.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2325"/>
    <dbReference type="EnsemblBacteria" id="ABA05579">
        <property type="protein sequence ID" value="ABA05579"/>
        <property type="gene ID" value="Nwi_2325"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2325"/>
    <dbReference type="eggNOG" id="COG2329">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141544_0_0_5"/>
    <dbReference type="OMA" id="WATRESY"/>
    <dbReference type="OrthoDB" id="1562645at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007138">
        <property type="entry name" value="ABM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03992">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51725">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABM" evidence="1">
        <location>
            <begin position="2"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="111" mass="12597" checksum="BAD848689D6B7CBD" modified="2005-10-11" version="1">MFIAMNRFQVKPGSEAAFENLWVTRESYLGAMPGFIEFHLLKGPKAEDHTLYSSHTTWVDRAAFEGWTKSEAFRKAHARADNKTGESLYLGHPKFEGFEIIQSERKSDAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ68</accession>
    <name>Q3SQ68_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Propeptide, PepSY amd peptidase M4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2319</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315538.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2319"/>
    <dbReference type="EnsemblBacteria" id="ABA05573">
        <property type="protein sequence ID" value="ABA05573"/>
        <property type="gene ID" value="Nwi_2319"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2319"/>
    <dbReference type="eggNOG" id="COG3182">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031962_3_2_5"/>
    <dbReference type="OMA" id="LAWRWHF"/>
    <dbReference type="OrthoDB" id="1656356at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005625">
        <property type="entry name" value="PepSY-ass_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34219">
        <property type="entry name" value="PTHR34219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03929">
        <property type="entry name" value="PepSY_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="193"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="378"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="420"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="461" mass="50544" checksum="E1F105301615A025" modified="2005-10-11" version="1">MSNSFSALALHRTVWRWHFYAGLVVAPFMFILAVTGAIYLFNDEINDVLHRDLRIVAPVSEPAPLSKMAEAAITAYPGGKVTRIDTARATNRSVEVFVTPATGDAVRVFVDPGTGRVLGSYVYARTLVGFADIAHGSLTLGSIGDNIVELAACWGFILVATGLYLWWPRGRFRLGGVLYPRIGRRGRPFWKEWHAVVGMWSAFLIMFLILTGLPWASFWGDLLRRGTDLAGIGYPANHRLQGTTPSTTTVKDAAGNAAPWTLEQAPIPDSDPHAGHHGHITSAPAAKRMLGVDDVAAILTREGLMAPYRLILPKNASGVFTAFSYPDQPETQRTLHVDQYSGRVIGDVRFADYGWAAKAVELGVQIHMGNYFGRLNQIVMLIPCIGIIVLTITGPLMWWRRRPKGEFAAPQPLSSPRLRTSAMIVIGLCVMFPLAGASLALAMIADWVWEQSPAYRLRPGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ70</accession>
    <name>Q3SQ70_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2317</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2317"/>
    <dbReference type="EnsemblBacteria" id="ABA05571">
        <property type="protein sequence ID" value="ABA05571"/>
        <property type="gene ID" value="Nwi_2317"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2317"/>
    <dbReference type="eggNOG" id="ENOG50301Y0">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147855_1_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021333">
        <property type="entry name" value="DUF2946"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11162">
        <property type="entry name" value="DUF2946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="134" mass="14561" checksum="C1DFEF6CAB4BF1D4" modified="2005-10-11" version="1">MTFVAKGGKIMRRRLGRFLPIVMLAMLVQIFAPVAVCLAFSPTTTDPLAGAICSHAPDTTSPQERETDPQQTHGACCALCCVVHHAAAPPPDPQAFEVRIERDATRVAWRDLIFDLRPLAARSNAQARAPPFIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ71</accession>
    <name>Q3SQ71_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF2231 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2316</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2316"/>
    <dbReference type="EnsemblBacteria" id="ABA05570">
        <property type="protein sequence ID" value="ABA05570"/>
        <property type="gene ID" value="Nwi_2316"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2316"/>
    <dbReference type="eggNOG" id="COG4244">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107155_2_0_5"/>
    <dbReference type="OMA" id="AGWHGGK"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019251">
        <property type="entry name" value="DUF2231_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09990">
        <property type="entry name" value="DUF2231"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2231" evidence="2">
        <location>
            <begin position="58"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="164" mass="17384" checksum="8F79A777BA921013" modified="2005-10-11" version="1">MTKSSMEQPVTAEEEEIPSAVAVARHPLHAMSVHFPIAFAIGALGSDALFLWTADPFWARVALWVLGFAFASGVAAGLVGTVELLLVPQIRHRVQSWSHGISAMMLIAILGTNWGLRLHVDDAVLPHGLLLSALGAIFTGLAGWHGGKLIFHHGVGVAISGNEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ77</accession>
    <name>Q3SQ77_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Short-chain dehydrogenase/reductase SDR</fullName>
            <ecNumber evidence="3">1.1.1.100</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2310</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.100" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2310"/>
    <dbReference type="EnsemblBacteria" id="ABA05564">
        <property type="protein sequence ID" value="ABA05564"/>
        <property type="gene ID" value="Nwi_2310"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2310"/>
    <dbReference type="eggNOG" id="COG4221">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_1_5"/>
    <dbReference type="OMA" id="PQFDWAR"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="337"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="371" mass="39762" checksum="3DDBB41C6F27ED87" modified="2005-10-11" version="1">MILTVGTSAIQGGVMFAIEQVFRKPIPESLQGKVVMITGGSAGVGRAAARAFAREGANVALLARDVAGLEDAKAEIESFGGEARIFPADVADAEAVFAAAAQCEVELGPIDIWVNNAMATVFSPVEQLTPEEIRRVTEVTYLGYVHGTLAALRHMRPRDRGVIVQVGSSLAYRGIPLQAAYCGAKHAVRGFTDSLRTELLHHRSNVAVTAVHLPAVNTPQFEWARTRMRKAFRPVAPVYTSRAAALAIVHAATHPKREYWLGGTTSLTIIGDMLFPGLLDRFLAREAVDGQQKETEIGANRSDNLFLPVSGRHRTNGAFSAEARPDPAMLSAQTIRFATIAGACVLIGFAGAVLGARRASAARTEKRCRPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQK1</accession>
    <name>Q3SQK1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2186</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315409.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05440">
        <property type="protein sequence ID" value="ABA05440"/>
        <property type="gene ID" value="Nwi_2186"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2186"/>
    <dbReference type="HOGENOM" id="CLU_1935800_0_0_5"/>
    <dbReference type="OMA" id="HRSIGEQ"/>
    <dbReference type="OrthoDB" id="2140051at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="132" mass="14754" checksum="41BEF3B8021B2236" modified="2005-10-11" version="1">MTSTDDVTNLVERLRQDAEMLKDGYQSNLTELWHGVVVKDAAKACAEAADLIESQAQAINNAKAEWDALDQSHRSIGEQLGNEGRRRIAAETRLKEAEKVVEMVAAMRINNSEQIRLVTRARAYLASKVKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQV6</accession>
    <name>Q3SQV6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">IstB-like ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2077</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2077"/>
    <dbReference type="EnsemblBacteria" id="ABA05335">
        <property type="protein sequence ID" value="ABA05335"/>
        <property type="gene ID" value="Nwi_2077"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2077"/>
    <dbReference type="eggNOG" id="COG1484">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4584">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_778085_0_0_5"/>
    <dbReference type="OMA" id="QMGIRTR"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002611">
        <property type="entry name" value="IstB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01695">
        <property type="entry name" value="IstB_IS21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IstB_IS21" evidence="2">
        <location>
            <begin position="211"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="319"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="356" mass="40286" checksum="80EE1C3B136392EC" modified="2005-10-11" version="1">MLVVETIARIRREHFIKGKTIRAIARDLKVSRNTVRKVLRSGETSFEYERQVQPRPKLGHWASELDVLLAANATKSAREQLTLIRIFEELRGRGYDGGYDAVRRYARRWNKERGQSTAAAYVPLSFAAGEAYQFDWSHEIVLLNGVTAIVKVAHVRLCHSRMLFVRAYPRETQEMVFDAHDRAFALFKGTCSRGIYDNMKTAVETIFVGKDRLEIESRNGRQGRIAEHLTRMDFIVLDELGYLPFAQSGGQLLFHLVSRLYERTSIVVTTNLAFGEWPSVFGDAKMTTALLDRLTHHCDIVETGNDSWRFKSRDDDHATRARPVSAIPASSDGPSATLKPRRAKGSNLDADTGSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQW0</accession>
    <name>Q3SQW0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Periplasmic binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2073</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2073"/>
    <dbReference type="EnsemblBacteria" id="ABA05331">
        <property type="protein sequence ID" value="ABA05331"/>
        <property type="gene ID" value="Nwi_2073"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2073"/>
    <dbReference type="eggNOG" id="COG0614">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038034_13_2_5"/>
    <dbReference type="OMA" id="VFPWDRY"/>
    <dbReference type="OrthoDB" id="1473468at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229209" evidence="1">
        <location>
            <begin position="31"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="2">
        <location>
            <begin position="50"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="343" mass="38343" checksum="3076E912C59877D2" modified="2005-10-11" version="1">MIQRRIDHSRAIALFACIMMLMSSMTPARADRTITDMAGRRVIIADRVARVATIGPVPVLNSFVFALGEARSLVNGLPPNLSGPRWRMQYLVDPDISRRPVLQGGGGAPLVEGLIETKPDIVLTMDRRNVDLAARARTPVVFLAWRQPNDVKAVMRLLGQIYGKTEAAEAYCSYFDMTLGNISTRLAQRSTQRPRVLYANLKRMTQPHLIAEWWIAQAGGRSVTDGDRPTEALTFSREQLLAWDPEVMILSNAAEVAEAYADPRLSTVSAIRNRRVAAIPTGVHLWGNRTVEEPLTVLWAAQLIHPDLFKDWDIKKEVVAFYAMFFKTSLAKPEVERILSGRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQW3</accession>
    <name>Q3SQW3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2070</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05328">
        <property type="protein sequence ID" value="ABA05328"/>
        <property type="gene ID" value="Nwi_2070"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2070"/>
    <dbReference type="eggNOG" id="ENOG50332KV">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2569208_0_0_5"/>
    <dbReference type="OMA" id="VTLTKMC"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="85" mass="9796" checksum="FFCEB81E5497E8D4" modified="2005-10-11" version="1">MDVQMKDSGLLHVTLTKMCELHAIDMPRNGELAEHFQLAFPSANYDEEADEWRMLWKKGTYAESNARLEAFFSERGVAFSHVDKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQW6</accession>
    <name>Q3SQW6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative L-sorbosone dehydrogenase (SNDH)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2067</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315304.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2067"/>
    <dbReference type="EnsemblBacteria" id="ABA05325">
        <property type="protein sequence ID" value="ABA05325"/>
        <property type="gene ID" value="Nwi_2067"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2067"/>
    <dbReference type="eggNOG" id="COG2133">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024435_0_0_5"/>
    <dbReference type="OMA" id="GINHHWT"/>
    <dbReference type="OrthoDB" id="1150223at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012938">
        <property type="entry name" value="Glc/Sorbosone_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011041">
        <property type="entry name" value="Quinoprot_gluc/sorb_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07995">
        <property type="entry name" value="GSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50952">
        <property type="entry name" value="SSF50952"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GSDH" evidence="1">
        <location>
            <begin position="200"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="456" mass="48908" checksum="F0E8EB02CF1CD28F" modified="2005-10-11" version="1">MKKLLISLLAIAFVAAIAGVGVILFSKEQATVALEEQYGSDPKLPPPDPRILPTVNQARAEPWKRGETPTAAQGYAVSQFADGLDHPRWLHVLPNGDVLVAESNKPPQDESSMGIRRWVAKLVMSSAGASTPSANRISILRDADGDGVAELRQPFITGLFSPFGMALLDGKLYVANANCIVAFPYQEGATEITAKPETITELPAGLNHHWTKDVIASPDGTKLYVTVGSNSNVGENGMEAEEGRAAVHEIDLATKQKRLFATGLRNPNGLSWQPDSGELWVVVNERDEIGSDLVPDYMTSVKEGAFYGWPYSYFGQHVDDRIEPRRPDLVAKAIKPDYALGSHTAALGLTFNSGSLFGPEMKNGAFIGQHGSWNRMPRSGYKVIFVPFSDGKPAGPPRDILTGFLRLDESAAGRPVGVAVARDGALLVADDVGNSIWRVTPSQSGQTNPGSVGSEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQX7</accession>
    <name>Q3SQX7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2056</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05314">
        <property type="protein sequence ID" value="ABA05314"/>
        <property type="gene ID" value="Nwi_2056"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2056"/>
    <dbReference type="HOGENOM" id="CLU_1233964_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="224" mass="25194" checksum="309ABBC9187A603E" modified="2005-10-11" version="1">MNFRVSDGGNDHYSRLTAGARRFCSLSGPLFTAHIRRSRIPQPAPRTAPRRQPDRFYLPWCGHALYSRRCPRGCPRILAKNCAIPKNRSLFASVVSNASLFKRASARSQLRIPGPEARSLKLPRTTTVDGLCTSLRGIVSNQPKKELWVTCKLTLLRHGEGKPPAKLMPRTHSQRDHFRWRASPALKRTIARCGWRVPKHPVSHLLRGSRKAPGSVWLGPRASC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="136" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR05</accession>
    <name>Q3SR05_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptide chain release factor 3</fullName>
            <shortName evidence="6">RF-3</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">prfC</name>
        <name evidence="9" type="ordered locus">Nwi_2027</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF-1 and RF-2 is significantly reduced by GTP and GDP, but not by GMP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. PrfC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315272.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2027"/>
    <dbReference type="EnsemblBacteria" id="ABA05286">
        <property type="protein sequence ID" value="ABA05286"/>
        <property type="gene ID" value="Nwi_2027"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2027"/>
    <dbReference type="eggNOG" id="COG4108">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002794_2_1_5"/>
    <dbReference type="OMA" id="TPASWPI"/>
    <dbReference type="OrthoDB" id="164090at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006449">
        <property type="term" value="P:regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04169">
        <property type="entry name" value="RF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00072">
        <property type="entry name" value="Rel_fac_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004548">
        <property type="entry name" value="PrfC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032090">
        <property type="entry name" value="RF3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038467">
        <property type="entry name" value="RF3_dom_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041732">
        <property type="entry name" value="RF3_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43556">
        <property type="entry name" value="PTHR43556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16658">
        <property type="entry name" value="RF3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00503">
        <property type="entry name" value="prfC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="8">
        <location>
            <begin position="21"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="216"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="30"/>
            <end position="37"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="98"/>
            <end position="102"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="152"/>
            <end position="155"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="539" mass="59400" checksum="E611884A83EBE369" modified="2005-10-11" version="1">MPDLALKTESTPASPLAAEVARRRTFAIISHPDAGKTTLTEKLLLFGGAINLAGQVKAKGERRNTRSDWMKIERDRGISVVTSVMTFEFDGRVFNLLDTPGHEDFSEDTYRTLTAVDSAVMVIDAAKGIEARTRKLFEVCRLRDIPIVTFINKMDRESRDTFELLDEIEKTLALDTTPMTWPVGRGRDFLGTYDIATGGIRLLEGGGAKTGAAERIDIADLAARNANLNVDEIREELALATEACKPFELEAFREGHLTPVYFGSALRNFGVGDLLEGLGNFAPPPRAQESNLRKVEADEPRMTAFVFKIQANMDPNHRDRIAFARLCSGKLTRGMKAKLVRTGKPMPLSSPQFFFAQDRAIADEAFAGDVVGIPNHGTLRIGDTLTEGEDVTFVGVPSFAPEIVRRVRLTDAMKAKKLKEALQQMSEEGVVQVFRPRDGAPALVGVVGMLQLDVLKARLDAEYSLPVDFEVCEFQLARWISSDDRKKLDVFIAANGSGVADDVDGDPVFLAKNEFYLGYTRERAEGIVFSSVKDVKKTS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR35</accession>
    <name>GATB_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B</fullName>
            <shortName evidence="1">Asp/Glu-ADT subunit B</shortName>
            <ecNumber evidence="1">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gatB</name>
        <name type="ordered locus">Nwi_1997</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + 2 H(+) + L-asparaginyl-tRNA(Asn) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14513"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="Rhea" id="RHEA-COMP:9677"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GatB/GatE family. GatB subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315243.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SR35"/>
    <dbReference type="SMR" id="Q3SR35"/>
    <dbReference type="STRING" id="323098.Nwi_1997"/>
    <dbReference type="EnsemblBacteria" id="ABA05256">
        <property type="protein sequence ID" value="ABA05256"/>
        <property type="gene ID" value="Nwi_1997"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1997"/>
    <dbReference type="eggNOG" id="COG0064">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019240_0_0_5"/>
    <dbReference type="OMA" id="ARKWWMG"/>
    <dbReference type="OrthoDB" id="497127at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050566">
        <property type="term" value="F:asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00121">
        <property type="entry name" value="GatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004413">
        <property type="entry name" value="Apn/Gln-ADT_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017959">
        <property type="entry name" value="Asn/Gln-tRNA_amidoTrfase_suB/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006075">
        <property type="entry name" value="Asn/Gln-tRNA_Trfase_suB/E_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018027">
        <property type="entry name" value="Asn/Gln_amidotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042114">
        <property type="entry name" value="GatB_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023168">
        <property type="entry name" value="GatB_Yqey_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017958">
        <property type="entry name" value="Gln-tRNA_amidoTrfase_suB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659">
        <property type="entry name" value="PTHR11659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02934">
        <property type="entry name" value="GatB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02637">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00845">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="SSF89095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00133">
        <property type="entry name" value="gatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01234">
        <property type="entry name" value="GATB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000241245" description="Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B">
        <location>
            <begin position="1"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00121"/>
        </source>
    </evidence>
    <sequence length="494" mass="53770" checksum="57B28A580516F340" modified="2005-10-11" version="1">MNAPVKPSHLIKGATGEWEMVIGIEVHAQVTSNAKLFSGASTAFGGDPNSHVSLVDAAMPGMLPVINEECVRQAVRTGLGLNARINLRSVFDRKNYFYPDLPQGYQISQYKSPIVGEGEVVVDLADGTSFVVGIERLHLEQDAGKSLHDQHADMSAIDLNRSGVALMEIVSRPDMRSSEQARAYVTKLRTILRYLGTCDGDMEKGNLRADVNVSVRRPGEPFGTRCEIKNVNSIRFIGQAIEYEARRQIGILEDGGGIDQETRLFDPDKGETRAMRSKEEAHDYRYFPDPDLLPLEFSQGLVDALKADLPELPDQKKARFVTDLGLTSYDAAVLVSEHESANFYESVLADLADARRDGKAAANWVINELFGRLNKQGLSIEASPVSASQLAAIIDLIGEGTISGKIAKDLFEIVWSEGGDPRALVEARGMKQVTDVGAIEKVVDDIVAANPDKVAQVRAKPQMIGWFVGQVMKASGGKVNPQAVNDLLKAKLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR55</accession>
    <name>Q3SR55_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1977</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315223.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1977"/>
    <dbReference type="EnsemblBacteria" id="ABA05236">
        <property type="protein sequence ID" value="ABA05236"/>
        <property type="gene ID" value="Nwi_1977"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1977"/>
    <dbReference type="HOGENOM" id="CLU_2634511_0_0_5"/>
    <dbReference type="OMA" id="LFPDRIW"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="75" mass="8055" checksum="449C78D6CCA8B4EB" modified="2005-10-11" version="1">MTSIKDKLASSVRQAKTGAESAPAEKAEVRSKKPRPDSGASKPAAAKPAPKEKETLADEPPESVQELFPDRIWPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR59</accession>
    <name>Q3SR59_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1973</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315219.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1973"/>
    <dbReference type="EnsemblBacteria" id="ABA05232">
        <property type="protein sequence ID" value="ABA05232"/>
        <property type="gene ID" value="Nwi_1973"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1973"/>
    <dbReference type="eggNOG" id="COG0265">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072546_1_0_5"/>
    <dbReference type="OMA" id="DQVNLMP"/>
    <dbReference type="OrthoDB" id="741829at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="SSF50494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="275" mass="30290" checksum="47A2EE4D9C682E4F" modified="2005-10-11" version="1">MEIDSIAKKLLFSTVRVDTELEDGSAGSGTAFILSHAHAKGNTPFIVTNRHLVEGVRRGGLVFTQTSEGRPAFGQRFQLNIDDFPAAWFLHPDPAVDLAIVPLRPLERAARDLGVQLYYHAIDSQRIPDAAAVRGFDALEDVLFVGYPSGVWDQVNLMPILRRGTTATPLELDFEGHKQFLIDAAVYPGSSGSPVFVVRDDPLRPRGDAARTIWFAGVVAEVFFREEANQLVPGPVPAQNDNRLIGSEMIDLGLVIKSESVRELIDAYVRKWLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR78</accession>
    <name>Q3SR78_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Site-specific DNA-methyltransferase (cytosine-N(4)-specific)</fullName>
            <ecNumber evidence="3">2.1.1.113</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_1953</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = an N(4)-methyl-2'-deoxycytidine in DNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:16857"/>
            <dbReference type="Rhea" id="RHEA-COMP:11369"/>
            <dbReference type="Rhea" id="RHEA-COMP:13674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:85452"/>
            <dbReference type="ChEBI" id="CHEBI:137933"/>
            <dbReference type="EC" id="2.1.1.113"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.113" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1953"/>
    <dbReference type="REBASE" id="25529">
        <property type="entry name" value="M.NwiORF1953P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05213">
        <property type="protein sequence ID" value="ABA05213"/>
        <property type="gene ID" value="Nwi_1953"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1953"/>
    <dbReference type="eggNOG" id="COG0863">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1625343_0_0_5"/>
    <dbReference type="OrthoDB" id="1071106at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015667">
        <property type="term" value="F:site-specific DNA-methyltransferase (cytosine-N4-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017985">
        <property type="entry name" value="MeTrfase_CN4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00093">
        <property type="entry name" value="N4_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="163" mass="18445" checksum="20D6256A0C4F8B4B" modified="2005-10-11" version="1">MEGAAIQGDSRDNLIIQKACELVGKFDWIITSPPYYGLRTYLPDQWIRNWFLGGPSVVDYTSEGQLSHNGRDRFIADLRQVWANVGARCRPGATLVIRFGSIGDRLVEDPAQLVSASLEETGWVTTEILHADNAARGRRQADTFHRKRSAPYDEVDVWAKWRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR83</accession>
    <name>Q3SR83_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Acetate kinase</fullName>
            <ecNumber evidence="11">2.7.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Acetokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ackA</name>
        <name evidence="13" type="ordered locus">Nwi_1948</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>acetate + ATP = acetyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:11352"/>
            <dbReference type="ChEBI" id="CHEBI:22191"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="11">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="11">Mg(2+). Can also accept Mn(2+).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="11">Metabolic intermediate biosynthesis; acetyl-CoA biosynthesis; acetyl-CoA from acetate: step 1/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Polyol metabolism; 1,2-propanediol degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Bacterial microcompartment</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PduL family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 11 12">Belongs to the acetokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.1" evidence="11"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315199.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1948"/>
    <dbReference type="EnsemblBacteria" id="ABA05208">
        <property type="protein sequence ID" value="ABA05208"/>
        <property type="gene ID" value="Nwi_1948"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1948"/>
    <dbReference type="eggNOG" id="COG0282">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020352_3_0_5"/>
    <dbReference type="OMA" id="SMRRRIC"/>
    <dbReference type="OrthoDB" id="440967at2"/>
    <dbReference type="UniPathway" id="UPA00340">
        <property type="reaction ID" value="UER00458"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00621"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031469">
        <property type="term" value="C:bacterial microcompartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008776">
        <property type="term" value="F:acetate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006085">
        <property type="term" value="P:acetyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051144">
        <property type="term" value="P:propanediol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00020">
        <property type="entry name" value="Acetate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004372">
        <property type="entry name" value="Ac/propionate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000890">
        <property type="entry name" value="Aliphatic_acid_kin_short-chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023865">
        <property type="entry name" value="Aliphatic_acid_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008300">
        <property type="entry name" value="PTAC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21060">
        <property type="entry name" value="PTHR21060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00871">
        <property type="entry name" value="Acetate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06130">
        <property type="entry name" value="PTAC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00471">
        <property type="entry name" value="ACETATEKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00016">
        <property type="entry name" value="ackA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01076">
        <property type="entry name" value="ACETATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-1283">Bacterial microcompartment</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11 12" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="11">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="201"/>
            <end position="205"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="275"/>
            <end position="277"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="323"/>
            <end position="327"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="380"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="11">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="11">
        <location>
            <position position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA05208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="589" mass="63594" checksum="3DF26DF4E6058517" modified="2005-10-11" version="1">MQVLVFNAGSSSLKFGIFSVATETREVFKGCYERFRAGGCEYRFRHGETDERGAAEFDSPGEALKGVPAALKRFGLDAIDAVGHRIVHGGARFSSPTLLDDETVEAIEALTPLAPLHNPTNLEAVRLCRGLWPDIRQVAVFDTAFHLSNPAFVTTYAVPLKWREAGLRRYGFHGTSHKYVAERAAQEIGRPLSDLQLVSIHLGNGASICAVNRGYSMDSSMGMTPLEGLVMGTRSGDVDPGAFGFLSRELQLSVQEIEDALYDESGLKGLAGSSDMRDIEDRAAKGDPQAQLTIEIYAYRAKKYVGAFAAAMGGLDAIVFTGGIGENSPSMRRRICDGLDFMGLQLDHDRNMAVNLSGRAAPQIQAYGSRVRVFVTETAEQLMIARETAAALAVVPPGGQLRLPVAVSARHVHLSREAVDGLFGKGYVLDQATPLRQPGHWAARERVTLVGPKGRLERVAILGPLRERTQIEVSRTDSFALGIDVPVRDSGRLEATPQVTIEGPAGSFTTDGLIIAARHIHTNPADAARQGIEDGEYVDVRVNDDDRTLTFGRTLVRVGKDSFTEVHIDTDEANAAGIEVAATGELVQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRF9</accession>
    <name>Q3SRF9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Hydrolase of the metallo-beta-lactamase superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_1872</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1872"/>
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        <property type="protein sequence ID" value="ABA05132"/>
        <property type="gene ID" value="Nwi_1872"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1872"/>
    <dbReference type="eggNOG" id="COG0595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008727_3_3_5"/>
    <dbReference type="OMA" id="KNTCVFE"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004613">
        <property type="entry name" value="RNase_J"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042173">
        <property type="entry name" value="RNase_J_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041636">
        <property type="entry name" value="RNase_J_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17770">
        <property type="entry name" value="RNase_J_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00649">
        <property type="entry name" value="MG423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Lactamase_B" evidence="8">
        <location>
            <begin position="22"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="557" mass="60051" checksum="9A65BDDA1F7DA497" modified="2005-10-11" version="1">MIARPDELMFAPLGGVGEIGMNLSIYGLGNRHQRSWLAVDLGVSFGDEEHLPGIDLIMPDIRFLEQERKNLVGLVLTHAHEDHFGAIIDLWPRLKCPIYATKFSAALFEAKCAAERNPPDIPVTVVPSGGRIEVGPFGVEFIPVAHSIPESHALAIHTAAGIVVHTGDWKIDPTPIIGPPTDERRLRELGDEGVLALIGDSTNAVRDGRSPSEAEVARTIADLVKAAKGRVAVTTFASNVGRLRAVADAAREAGREVVVVGRAMARVVQVARETGHLNGMQDFRSADVYGHLPHDKVLALCTGSQGEPRAALARIARDDHPEVTLNRGDTVIFSSRTIPGNEKAVGAIINGLVAQGIEVITDRTQLVHVSGHPRCDELRDMISWVRPGLLIPVHGEALHLSEHARLARASGVPKVLICKNGDLVRLGPGEPGIIDELPSGRLYKDGAILEDSKSRAVVERRRMAFAGCVFVAIAVTAKGELIEDPEVDLVGIPEKNMAGEVLDDIVFDTVVSTAKGFSRTLRRDPDAMAESLRRAVRAAVAEQWGKKPLCYVHVLVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRJ6</accession>
    <name>Q3SRJ6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Triosephosphate isomerase</fullName>
            <shortName evidence="5">TIM</shortName>
            <shortName evidence="5">TPI</shortName>
            <ecNumber evidence="5 6">5.3.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Triose-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">tpiA</name>
        <name evidence="7" type="ordered locus">Nwi_1835</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:18585"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="5.3.1.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-erythrulose 1-phosphate = D-erythrulose 4-phosphate</text>
            <dbReference type="Rhea" id="RHEA:49588"/>
            <dbReference type="ChEBI" id="CHEBI:58002"/>
            <dbReference type="ChEBI" id="CHEBI:90796"/>
            <dbReference type="EC" id="5.3.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5 6">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5 6">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; erythritol degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the triosephosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.1" evidence="5 6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315091.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1835"/>
    <dbReference type="EnsemblBacteria" id="ABA05095">
        <property type="protein sequence ID" value="ABA05095"/>
        <property type="gene ID" value="Nwi_1835"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1835"/>
    <dbReference type="eggNOG" id="COG0149">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024251_2_1_5"/>
    <dbReference type="OMA" id="QEVCGAI"/>
    <dbReference type="OrthoDB" id="1266295at2"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00189"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="UniPathway" id="UPA01066"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004807">
        <property type="term" value="F:triose-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00311">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00147_B">
        <property type="entry name" value="TIM_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035990">
        <property type="entry name" value="TIM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022896">
        <property type="entry name" value="TrioseP_Isoase_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000652">
        <property type="entry name" value="Triosephosphate_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020861">
        <property type="entry name" value="Triosephosphate_isomerase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139">
        <property type="entry name" value="PTHR21139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00121">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51351">
        <property type="entry name" value="SSF51351"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00419">
        <property type="entry name" value="tim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00171">
        <property type="entry name" value="TIM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51440">
        <property type="entry name" value="TIM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5 6" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="5 6" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="4 5 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="11"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="233"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="active site" description="Electrophile" evidence="5">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="250" mass="25500" checksum="6F7E37E00D4B17C2" modified="2005-10-11" version="1">MTGLRPLIAGNWKMNGLKASASELGQIISGAAGPAQKADLLICPPATLVLAFVGAVQGSPVGIGGQDCHPKPSGAHTGDISAEMLGDAGATAVIVGHSERRADHAESDALVREKAEAAWRAGLTAIVCVGETQDQRDAGQTLDVVGRQLTGSLPDGATAANLVVAYEPVWAIGTGRTPTSQDVQEVHRFIREKLAERFSAEGTKIRILYGGSVKPSNAAELLAVPNVNGALIGGASLKAADFLGIAQAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRJ9</accession>
    <name>Q3SRJ9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1832</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315088.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1832"/>
    <dbReference type="EnsemblBacteria" id="ABA05092">
        <property type="protein sequence ID" value="ABA05092"/>
        <property type="gene ID" value="Nwi_1832"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1832"/>
    <dbReference type="eggNOG" id="COG4104">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135001_0_0_5"/>
    <dbReference type="OMA" id="DSTANCK"/>
    <dbReference type="OrthoDB" id="1960797at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008727">
        <property type="entry name" value="PAAR_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05488">
        <property type="entry name" value="PAAR_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="118" mass="11612" checksum="AD26554A0589410D" modified="2005-10-11" version="1">MRLPLVVSIATASAFIGTVQPGPARSQTPNPLAQSPGVITGGSQNVMVNGKPAARQGDATTGGALIEGSSNVFINGKPATVIGNRTGCGGSVTSGGHGVFINGKPMARQGDQPSGCAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRK8</accession>
    <name>Q3SRK8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1822</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315079.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1822"/>
    <dbReference type="EnsemblBacteria" id="ABA05083">
        <property type="protein sequence ID" value="ABA05083"/>
        <property type="gene ID" value="Nwi_1822"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1822"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035702_1_0_5"/>
    <dbReference type="OMA" id="FYIPGYD"/>
    <dbReference type="OrthoDB" id="1639760at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="418" mass="46539" checksum="5D43C2FE24E53EFC" modified="2005-10-11" version="1">MIARRHVFHIGGYDPISPAQQVSRLARSLSVFQRTWGVRAGIVSAIDATPVSASWIVEASGPNWSTRTSYEMLRWDDLILEDHARGMWSRLWRSFVTLVDFIVTGTLFRFMYANWKYAGFFLFPYLCVAGFIGCGVAAGYGLVRLTGVSGFPAGVLGAATAVGVGAGLLRLLGSRRPINHVFDDWIFSREFVYGRRPRMDARIDRFAERILDRVRNADVDEVVIIGHCLGAALVMDAVARALKRDPELAGRGRSLCILTVGSTIAKFSLHPRGEHIRQAAQAVAGEMRIPWTEYHARDDAISFYRFDPVMLKRVSRERSGGRPDIRRVQLHDMMDRASFRRHRFNFMLLHYKFLMGNSVRANYDYCLIICGPLPFEEITSPRGGVDRFGEDGALLQRASSGTAAAASRSADAAVAGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRP4</accession>
    <name>Q3SRP4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1786</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315043.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1786"/>
    <dbReference type="EnsemblBacteria" id="ABA05047">
        <property type="protein sequence ID" value="ABA05047"/>
        <property type="gene ID" value="Nwi_1786"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1786"/>
    <dbReference type="eggNOG" id="ENOG503396A">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1979853_0_0_5"/>
    <dbReference type="OMA" id="CKTRLLM"/>
    <dbReference type="OrthoDB" id="2011088at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="126" mass="13448" checksum="35366EBECDD2D46A" modified="2005-10-11" version="1">MKKDPFRLTPYQVQWLLIVGFLSVGYALYLRYLAIELSTVSLACDSGLQTMLCRGRSVATYLFRNQVFGIAALVVAVLHVMRPSIVLLTAGLSAAGFGIVLYNAGLSGLAIGLLILGFARPAPATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRU6</accession>
    <name>Q3SRU6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">FecI like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_1734</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1734"/>
    <dbReference type="EnsemblBacteria" id="ABA04995">
        <property type="protein sequence ID" value="ABA04995"/>
        <property type="gene ID" value="Nwi_1734"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1734"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_12_3_5"/>
    <dbReference type="OMA" id="MREPRAY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="3">
        <location>
            <begin position="77"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="4">
        <location>
            <begin position="180"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="236" mass="26729" checksum="A0B30E9F771F35AB" modified="2005-10-11" version="1">MRLRKNFGRHKRLEADHRDGDSVSASSSLASSRSHHDPFRFRSRLSAAAVAGRIRLRARDGFRLAFGARMSWDLHRLFLRHARDITAALRRRGLSEETAADITQDTFVKVLVSPPGRSVTVHNPAAYLFRVARNLGIDHQRRERVLRRVEMSAEDFAAIVDPTPSPESAVYDRQKLALTRAALAELPERTRVAFEMHRLDEMTIAEVAAELGLSTSRVWSLIRDAYDHIDARLTGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS25</accession>
    <name>Q3SS25_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Monooxygenase, FAD-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1655</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1655"/>
    <dbReference type="EnsemblBacteria" id="ABA04916">
        <property type="protein sequence ID" value="ABA04916"/>
        <property type="gene ID" value="Nwi_1655"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1655"/>
    <dbReference type="eggNOG" id="COG0654">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033626_0_0_5"/>
    <dbReference type="OMA" id="ALMPQWD"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_3" evidence="1">
        <location>
            <begin position="14"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="419" mass="45754" checksum="C692E6A7B7415154" modified="2005-10-11" version="1">MDAASPRQQRSMSTRCCIVGGGPAGMMLGYLLARAGVDVVVLEKHADFFRDFRGDTVHPSTMQVMDELGLIDDFLKVPHDKLQKLDAHFGGRRLRIGDISRTGARYPFIAFMPQWDFLNFLREKGRRYPNLKLMMGTEGKGLVQDGDTVIGVHAEDADGAFEIMADLTVACDGRHSVLRASAGLEVEEIGAPMDVLWFRAGKSTETESILARIEAGKMMVTLDRGTYWQCAYVIPKGQHDAVKARGLESFRSDVVSLAPILKSGIADVKSWDDVKLLTVAVNRLKRWTRPGLLCIGDAAHAMSPIGGVGVNIAIQDAVAAANHLAGKLKRGGVSEHDLAEVQRRREFPMRMTQAMQVTVQNNIVSAALKPGEGPFKPPLFARVVNAVPFLQGLTARFIAVGVRPEHVRSEEAGLSEARS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS86</accession>
    <name>PPK1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Polyphosphate kinase</fullName>
            <ecNumber evidence="1">2.7.4.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP-polyphosphate phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Polyphosphoric acid kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ppk</name>
        <name type="ordered locus">Nwi_1594</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible transfer of the terminal phosphate of ATP to form a long-chain polyphosphate (polyP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[phosphate](n) + ATP = [phosphate](n+1) + ADP</text>
            <dbReference type="Rhea" id="RHEA:19573"/>
            <dbReference type="Rhea" id="RHEA-COMP:9859"/>
            <dbReference type="Rhea" id="RHEA-COMP:14280"/>
            <dbReference type="ChEBI" id="CHEBI:16838"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="PTM">
        <text evidence="1">An intermediate of this reaction is the autophosphorylated ppk in which a phosphate is covalently linked to a histidine residue through a N-P bond.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the polyphosphate kinase 1 (PPK1) family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.1" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314859.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SS86"/>
    <dbReference type="SMR" id="Q3SS86"/>
    <dbReference type="STRING" id="323098.Nwi_1594"/>
    <dbReference type="PRIDE" id="Q3SS86"/>
    <dbReference type="EnsemblBacteria" id="ABA04855">
        <property type="protein sequence ID" value="ABA04855"/>
        <property type="gene ID" value="Nwi_1594"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1594"/>
    <dbReference type="eggNOG" id="COG0855">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009678_5_0_5"/>
    <dbReference type="OMA" id="NIKWARK"/>
    <dbReference type="OrthoDB" id="76567at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009358">
        <property type="term" value="C:polyphosphate kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008976">
        <property type="term" value="F:polyphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006799">
        <property type="term" value="P:polyphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1840.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00347">
        <property type="entry name" value="Polyphosphate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003414">
        <property type="entry name" value="PP_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041108">
        <property type="entry name" value="PP_kinase_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024953">
        <property type="entry name" value="PP_kinase_middle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036830">
        <property type="entry name" value="PP_kinase_middle_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025200">
        <property type="entry name" value="PPK_C_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025198">
        <property type="entry name" value="PPK_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036832">
        <property type="entry name" value="PPK_N_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30218">
        <property type="entry name" value="PTHR30218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02503">
        <property type="entry name" value="PP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13090">
        <property type="entry name" value="PP_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17941">
        <property type="entry name" value="PP_kinase_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13089">
        <property type="entry name" value="PP_kinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015589">
        <property type="entry name" value="PP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140356">
        <property type="entry name" value="SSF140356"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03705">
        <property type="entry name" value="poly_P_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000120504" description="Polyphosphate kinase">
        <location>
            <begin position="1"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="703"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="715"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphohistidine intermediate" evidence="1">
        <location>
            <position position="453"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="393"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="423"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="486"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="582"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="610"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="733" mass="82103" checksum="0418C9C3E5CF5880" modified="2005-10-11" version="1">MDSIQAIAPEEKKPELELDSAIRASPERFINRELSWLHFNRRVLEESVNPGHPALERVRFLSISANNLDEFFMVRVAGIKAQVREGITERSPDGLTPSEQLVLINETVSDLASDQQAIWRDLRNMLAGVGIVLIDGKDVTKAERSWIADHFLHNIFPLLTPLAIDPAHPFPFIPSLGFTIALQLARTSDGKPMNALIRMPGKIDRFLRIPAASKDDPVRLITIEGATSLFINRLFPGYTVKGQGAFRIIRDSELEIEEEAEDLVRLFETALKRRRRGSVIRLEIEAAMPAELRAFVQRALSAADDEVLLVDGVLAMNELSQLTRLDRPDLEFVPYVPRHPERVRDHGGDVFAAIRQKDLIVHHPYESFDVVVQFLQQAARDPDVVAIKQTLYRTSNNSPIVRALAEAAEAGKSVTALIELKARFDEEANIRWARDLERAGVQVVYGFLELKTHAKLSLVVRREGGSLATYVHTGTGNYHPVTARIYTDLSYFTSDPIIGRDAARVFNYITGYAEPSDIERMAVSPLTLRNRILDHIRGETTFARNGKPAAVWMKMNALVDPDIIDALYEASQAGVSVELIVRGICCLRPGVPGLSENIRVKSVIGRFLEHGRIYCFGMGQTMPGPKAAVYISSADMMPRNLDRRVEVLCPLQNATVHQQVLEQIMVANLKDTEQSWRLLPDGSSTRMKAAKGEEPFNLHNYFMTNPSLSGRGKSLKESSPRRLTRRSERQPPA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSH7</accession>
    <name>PXPA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">5-oxoprolinase subunit A</fullName>
            <shortName evidence="1">5-OPase subunit A</shortName>
            <ecNumber evidence="1">3.5.2.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5-oxoprolinase (ATP-hydrolyzing) subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pxpA</name>
        <name type="ordered locus">Nwi_1503</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the cleavage of 5-oxoproline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-oxo-L-proline + ATP + 2 H2O = ADP + H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10348"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58402"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.5.2.9"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a complex composed of PxpA, PxpB and PxpC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LamB/PxpA family.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.9" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314770.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSH7"/>
    <dbReference type="SMR" id="Q3SSH7"/>
    <dbReference type="STRING" id="323098.Nwi_1503"/>
    <dbReference type="EnsemblBacteria" id="ABA04764">
        <property type="protein sequence ID" value="ABA04764"/>
        <property type="gene ID" value="Nwi_1503"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1503"/>
    <dbReference type="eggNOG" id="COG1540">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069535_0_0_5"/>
    <dbReference type="OMA" id="DRTYKQD"/>
    <dbReference type="OrthoDB" id="918580at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017168">
        <property type="term" value="F:5-oxoprolinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00691">
        <property type="entry name" value="PxpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005501">
        <property type="entry name" value="LamB/YcsF/PxpA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30292">
        <property type="entry name" value="PTHR30292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03746">
        <property type="entry name" value="LamB_YcsF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000045213" description="5-oxoprolinase subunit A">
        <location>
            <begin position="1"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00691"/>
        </source>
    </evidence>
    <sequence length="255" mass="26808" checksum="3003C962F321BE68" modified="2005-10-11" version="1">MTIDLNCDLGESFGVWPMGSDAAMIELATSVNIACGFHAGDADTMRKTVDLAGTHGVNIGAHPGYRDLHGFGRRPIAGLTSGEIENLVAYQIGALQAIATMAGHRVTHVKAHGALSNVACEDDMTARAIASAVKAVDPGLVFVVPANSRLVAAGEAAGLPLAHEVFADRAYEDDGSLVSRRKPGAVLHNADEIARRVVTMLQSGEVVSITGKPIRMRMDTVCIHGDTPDAVRIARTLRQALKDNGIAVAPFNLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSJ4</accession>
    <name>Q3SSJ4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RecA-family ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1486</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1486"/>
    <dbReference type="EnsemblBacteria" id="ABA04747">
        <property type="protein sequence ID" value="ABA04747"/>
        <property type="gene ID" value="Nwi_1486"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1486"/>
    <dbReference type="eggNOG" id="COG3598">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_352962_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039459">
        <property type="entry name" value="RepB-like_DNA_primase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16793">
        <property type="entry name" value="RepB_primase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RepB_primase" evidence="1">
        <location>
            <begin position="106"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="796" mass="87206" checksum="10DA64621AF45AB7" modified="2005-10-11" version="1">MLMTHNTPQTADSASTFDRTTIRNHFWMLHHLAERANVPGKLVLCAIADKPLGLPPINQHFAIGDVDGMTAAAMAFQGTQYGVYDSRGLMRPDLPEGAKGTQADVVRTLAFTVDADSDHEGVATPHAPLMPNYIVESSKGNYQEVMILDRALPPAESKPLARALGELTGDKMCVPDISRIMRVPGTLNWPSTPKIKRGRSPDPQPVRVHKAWNSWTKVDDLLAVVAANVKPKTIKITAPVPDAVDGVSHDGIRYAEVVWYCQHMAKVWTPDDRETTPDDFELAKRIKLSFPGEDGLNAVLLLGWEDREDELRKRWWNPNDFKTEGENLLTLRGLKEVNWMFRHVIGCPYPPAPPFSPLPIGSPLPEGWRWTEDGRTERTAPSLPGGILPVFSAASLEGLPVPPRRWHVRDWIPAETVTLLYGDGGTGKSLIALQLLASTALGRPWLGRVVEKGACLFVTAEDSRDEVHRRLADIAREHSVPLSKMTDLHIVSLAGEDAILAAPDGRSSILIPTALFAALEAQCIALRPTFVVLDTLADLFGGNEIDRSQARQFIGLLRGLALKYDITILLLAHPSVSGMTRGTGSSGSTGWNNSVRSRLYFDRIRADDNSEPDPDARVLRSMKSNYGRVGEEIVLHWRRGVFVPDSITAGGLMAANDARNRADEVFLKMLALYDAESRGAVSPNPGSNYAPKLFAEHAGAQGLKSRALKDAMDRLLASKRIEAIMTGPPSRMRQHLIVSDRTYQLTKEPTNPIPTPCQPATNGVPTPLPTVPQTLPTPIPTGCYPNPHTPIALRAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSK2</accession>
    <name>Q3SSK2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized phage protein (Possible DNA packaging)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1478</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314746.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1478"/>
    <dbReference type="EnsemblBacteria" id="ABA04739">
        <property type="protein sequence ID" value="ABA04739"/>
        <property type="gene ID" value="Nwi_1478"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1478"/>
    <dbReference type="eggNOG" id="ENOG5032WN3">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085951_6_4_5"/>
    <dbReference type="OMA" id="FECRNIA"/>
    <dbReference type="OrthoDB" id="1829390at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd08054">
        <property type="entry name" value="gp6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021146">
        <property type="entry name" value="Phafe_gp6_head-tail"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05135">
        <property type="entry name" value="Phage_connect_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="106" mass="11885" checksum="ACB733A811E0A88A" modified="2005-10-11" version="1">MIATLADAKAQLNVTSDIDDTLLTRKVSAAQDYLESWLGYKIESIYGGVDQEPVPPALVEAVLQLVGHWYENREATLVGVSAQQLPHGVSDIIREYRRWWSTPDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="139" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSN5</accession>
    <name>Q3SSN5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 11">DNA-binding transcriptional regulator NtrC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Nitrogen regulation protein NR(I)</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ntrC</name>
        <name evidence="14" type="ordered locus">Nwi_1445</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Member of the two-component regulatory system NtrB/NtrC, which controls expression of the nitrogen-regulated (ntr) genes in response to nitrogen limitation. Phosphorylated NtrC binds directly to DNA and stimulates the formation of open promoter-sigma54-RNA polymerase complexes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314714.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1445"/>
    <dbReference type="EnsemblBacteria" id="ABA04706">
        <property type="protein sequence ID" value="ABA04706"/>
        <property type="gene ID" value="Nwi_1445"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1445"/>
    <dbReference type="eggNOG" id="COG2204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_0_1_5"/>
    <dbReference type="OMA" id="DWTGNIR"/>
    <dbReference type="OrthoDB" id="123059at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000156">
        <property type="term" value="F:phosphorelay response regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009399">
        <property type="term" value="P:nitrogen fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002197">
        <property type="entry name" value="HTH_Fis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002078">
        <property type="entry name" value="Sigma_54_int"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025943">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025944">
        <property type="entry name" value="Sigma_54_int_dom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010114">
        <property type="entry name" value="Transcript_reg_NtrC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02954">
        <property type="entry name" value="HTH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00158">
        <property type="entry name" value="Sigma54_activat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01590">
        <property type="entry name" value="HTHFIS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01818">
        <property type="entry name" value="ntrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00676">
        <property type="entry name" value="SIGMA54_INTERACT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00688">
        <property type="entry name" value="SIGMA54_INTERACT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50045">
        <property type="entry name" value="SIGMA54_INTERACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 11" id="KW-0010">Activator</keyword>
    <keyword evidence="4 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-0535">Nitrogen fixation</keyword>
    <keyword evidence="3 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0678">Repressor</keyword>
    <keyword evidence="9 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 11" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="13">
        <location>
            <begin position="5"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma-54 factor interaction" evidence="12">
        <location>
            <begin position="140"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="10">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABA04706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="479" mass="52719" checksum="1F002092F3556F8C" modified="2005-10-11" version="1">MSPANILVADDDGAIRTVINQALSRAGYDVRLTGNAATLWRWVSQGEGDIVITDVVMPDENAFDLLPRIKKIRPNLPVIVMSAQNTFMTAIRASERGAYEYLPKPFDLKELIAIVGRALAEPKESAASRGDENEGDSLPLIGRSPAMQEIYRVLARLMQTDLTVMISGESGTGKELVARALHDYGKRRGGPFVAVNMAAIPRDLIESELFGHERGAFTGANARASGRFEQAEGGTLFLDEIGDMPMEAQTRLLRVLQQGEYCTVGGRTAIKTDVRIVAASNKDLRVLIQQGLFREDLFFRLNVVPLRLPPLRERIEDLPDLIRHFFVLAEKDGLPPKKLDALALERLKRHRWPGNVRELENLARRLAALYPQDVITAPVIDCELASPEATSGSNAQNINSLGGAVEMYMSSLFSGSPNGLPPPGLYQRVLREIEVPLLTAVLAATRGNQIRAADLLGLNRNTLRKKIRDLDIQVYRSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSN7</accession>
    <name>Q3SSN7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA-dihydrouridine synthase</fullName>
            <ecNumber evidence="6">1.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_1443</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the dus family.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314712.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1443"/>
    <dbReference type="EnsemblBacteria" id="ABA04704">
        <property type="protein sequence ID" value="ABA04704"/>
        <property type="gene ID" value="Nwi_1443"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1443"/>
    <dbReference type="eggNOG" id="COG0042">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013299_0_1_5"/>
    <dbReference type="OMA" id="RPWLFAD"/>
    <dbReference type="OrthoDB" id="1710586at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017150">
        <property type="term" value="F:tRNA dihydrouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001269">
        <property type="entry name" value="DUS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004652">
        <property type="entry name" value="tRNA_dU_NifR3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006621">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00737">
        <property type="entry name" value="nifR3_yhdG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 6" id="KW-0288">FMN</keyword>
    <keyword evidence="5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Dus" evidence="8">
        <location>
            <begin position="30"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR006621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR006621-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA04704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="349" mass="37453" checksum="392FFC0A273FFB8E" modified="2005-10-11" version="1">MSKSLTGPPPSSSLGPFAVGDISIRNRVLLAPMSGITDRPFRRLTESLGAALVVSEMTASDDLVRGRPMSVLRCEATGHGPHVVQLAGCETRWMAEAARIAEAAGADIIDINMGCPARHVTGGQSGSALMRDPDHALDLIEATVGAVNVPVTLKMRLGWDGHSFNAPSLARRAESAGVRMITVHGRTRCQFYKGRADWRAVRAVKEAVRVPVVVNGDITSFDAAVAALEASGADAVMVGRGAQGRPWLPGQIGRRLETGIEESHPSLTDQLAYIRALYDDLLLHYGLRIGLRHARKHLGWALDTAAALRAVPTPVQKSWRTKILTADDPSGVQRLLVDAFDDFAWRAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSP6</accession>
    <name>Q3SSP6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Type I secretion outer membrane protein, TolC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1434</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the outer membrane factor (OMF) (TC 1.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1434"/>
    <dbReference type="EnsemblBacteria" id="ABA04695">
        <property type="protein sequence ID" value="ABA04695"/>
        <property type="gene ID" value="Nwi_1434"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1434"/>
    <dbReference type="eggNOG" id="COG1538">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012817_0_1_5"/>
    <dbReference type="OMA" id="LDCFESK"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019867">
        <property type="term" value="C:outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003423">
        <property type="entry name" value="OMP_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010130">
        <property type="entry name" value="T1SS_OMP_TolC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02321">
        <property type="entry name" value="OEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01844">
        <property type="entry name" value="type_I_sec_TolC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="351"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="490" mass="52810" checksum="079FBFA22B8147F7" modified="2005-10-11" version="1">MIRRRGSALFTSCDMKNGILRMGGVRLAAAAAVLAFMTAWPGTATRVSADTIESALVRAYQSNPQLNAQRAAVRATDESVPQALSGYRPRVAVTTSGGFQHTDMTFSQGLPGQGNVLGRIKGNQTPVAIGATVSQTLFDGFQAANRTRAAEGQVSAAREGLRVLEQTVLLAAATIYMDYLRDAAIVEVQRSNVRVLEETLKQTRDRFNVGEVTRTDVAQSEAQLAAGRTQLMATESTLITTRANYRRIVGNEPGQLAPGSPVDRFLPSTLARSVDLALTQNPNVTAAMYGIDVSHLQVKVAEGVLFPTVTLQANVQKGYDQQLTLPTQFAASAIAQVSVPIYQGGAEYSLIRQSKETLAQQRLNLEQVRNQARASTVQAWGQLEATKVQVKSAQAQVEASEIAVNGIREEAKAGQRTTFDVLVAQQALVSARVALVTAQHDRVVASYAVLNAVGRLTPQVLKLNTTVYDPSVHYHQVRDSWIGLRTPDGQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSS4</accession>
    <name>PLSX_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphate acyltransferase</fullName>
            <ecNumber evidence="1">2.3.1.274</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Acyl-ACP phosphotransacylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Acyl-[acyl-carrier-protein]--phosphate acyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphate-acyl-ACP acyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">plsX</name>
        <name type="ordered locus">Nwi_1406</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible formation of acyl-phosphate (acyl-PO(4)) from acyl-[acyl-carrier-protein] (acyl-ACP). This enzyme utilizes acyl-ACP as fatty acyl donor, but not acyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a fatty acyl-[ACP] + phosphate = an acyl phosphate + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:42292"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
            <dbReference type="EC" id="2.3.1.274"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; phospholipid metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Probably interacts with PlsY.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">Associated with the membrane possibly through PlsY.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PlsX family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.274" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314676.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSS4"/>
    <dbReference type="SMR" id="Q3SSS4"/>
    <dbReference type="STRING" id="323098.Nwi_1406"/>
    <dbReference type="EnsemblBacteria" id="ABA04667">
        <property type="protein sequence ID" value="ABA04667"/>
        <property type="gene ID" value="Nwi_1406"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1406"/>
    <dbReference type="eggNOG" id="COG0416">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039379_1_0_5"/>
    <dbReference type="OMA" id="HGKSNAR"/>
    <dbReference type="OrthoDB" id="631784at2"/>
    <dbReference type="UniPathway" id="UPA00085"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043811">
        <property type="term" value="F:phosphate:acyl-[acyl carrier protein] acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00019">
        <property type="entry name" value="PlsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003664">
        <property type="entry name" value="FA_synthesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012281">
        <property type="entry name" value="Phospholipid_synth_PlsX-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30100">
        <property type="entry name" value="PTHR30100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02504">
        <property type="entry name" value="FA_synthesis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002465">
        <property type="entry name" value="Phsphlp_syn_PlsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00182">
        <property type="entry name" value="plsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000001796" description="Phosphate acyltransferase">
        <location>
            <begin position="1"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00019"/>
        </source>
    </evidence>
    <sequence length="353" mass="37101" checksum="5872479C6EED52B5" modified="2005-10-11" version="1">MPQKVRIALDAMGGDVGASVVIPGAAISLNRHPDTEFLLYGNGALIEAQLAAHPAMKAVSRVIHTDVAVGMDDKPSQALRRGRKTSSMWLAIDAVKKGEADVAVSAGNTGALMAMARFNLRTLPGIDRPAIAAVWPTLRGDSVVLDLGATIGGDAHHLAALAIMGSAMARVLFDLERPTVGLLNIGVEEVKGGEEIREAAELLRSLNLPQLDFIGFVEGDGIGRGAADVIVTEGFSGNIALKAAEGTARQIAEYLRSAMSRTLLSRIGYLFARGAFNALRGKMDPRKSNGGVFLGLNGIVVKSHGGTDAEGFAYAVDVGYDMVRYDLLTKINQTLNRHGHTLAPASAVQEALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STD3</accession>
    <name>Q3STD3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two component transcriptional regulator, winged helix family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_1196</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314487.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1196"/>
    <dbReference type="EnsemblBacteria" id="ABA04458">
        <property type="protein sequence ID" value="ABA04458"/>
        <property type="gene ID" value="Nwi_1196"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1196"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_1_5"/>
    <dbReference type="OMA" id="WQQSYGD"/>
    <dbReference type="OrthoDB" id="1020672at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="16"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="4">
        <location>
            <begin position="139"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="139"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="238" mass="27017" checksum="5D0C82AE413654C7" modified="2005-10-11" version="1">MVMERQPLDNSSNQMRLLIIEDDRESADYLVKAFREVGHIAELARDGEEGLSFADGGDYDVLVIDRMLPKRDGLSVIGCLREKGILTPVLILSALGQVDDRIKGLRAGGDDYLPKPYSFAELLARVEVLSRRHGGRAEETLYRVGDLELDRLSRRVARGGEELTLQPREFRLLEYLMKHAGQVVTRTMLLENVWDYHFDPQTNVIDVHISRLRSKIDKGFEQPLLHTIRGAGYMVRSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STG0</accession>
    <name>Q3STG0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF2019 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1169</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314460.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1169"/>
    <dbReference type="EnsemblBacteria" id="ABA04431">
        <property type="protein sequence ID" value="ABA04431"/>
        <property type="gene ID" value="Nwi_1169"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1169"/>
    <dbReference type="eggNOG" id="ENOG5033ZRP">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165467_0_0_5"/>
    <dbReference type="OMA" id="VALFAKI"/>
    <dbReference type="OrthoDB" id="1764604at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018568">
        <property type="entry name" value="DUF2019"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09450">
        <property type="entry name" value="DUF2019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="SSF48371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2019" evidence="1">
        <location>
            <begin position="13"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="123" mass="13731" checksum="AE9AEC818E4D1010" modified="2005-10-11" version="1">MTPVQSDGARVQGLIARFAEIGMAQYDAIYVINTIKYNRLYAEMKRVWTELKEMPGDQRTALLPLLSHTNVQVRLMAAHSLLALYPGLARRTLETIRDSGIEPQNGSAANAIRRLDEGSYVPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STH5</accession>
    <name>Q3STH5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1154</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1154"/>
    <dbReference type="EnsemblBacteria" id="ABA04416">
        <property type="protein sequence ID" value="ABA04416"/>
        <property type="gene ID" value="Nwi_1154"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1154"/>
    <dbReference type="HOGENOM" id="CLU_1309063_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="210" mass="23561" checksum="5421EF2C9A958894" modified="2005-10-11" version="1">MRTRALIRRLVSSISFFEFFDMRDLDFNPNNLPKDVLEALGLITACSAHSDYVIESGIAGCLGVNIEYGSAVTTHMTTPHRKDVFLAAAELRIDDVHILIHLEDLLDELVDALKVRNGYVHNSPAIDRSTNETFMIRTEARGSVDVSLIPLSVEVILRDAQRIYKAGISLYGLLDKLGLLPKIPDGVRPNRTHKDKAARKKIRKSRRDRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STK5</accession>
    <name>Q3STK5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Flagellar motor switch protein FliM</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_1124</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">FliM is one of three proteins (FliG, FliN, FliM) that forms the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the motor that determine the direction of flagellar rotation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell inner membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Bacterial flagellum basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the FliM family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314417.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1124"/>
    <dbReference type="EnsemblBacteria" id="ABA04386">
        <property type="protein sequence ID" value="ABA04386"/>
        <property type="gene ID" value="Nwi_1124"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1124"/>
    <dbReference type="eggNOG" id="COG1868">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052646_2_0_5"/>
    <dbReference type="OMA" id="NPQFTQI"/>
    <dbReference type="OrthoDB" id="1748746at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17908">
        <property type="entry name" value="FliM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028976">
        <property type="entry name" value="CheC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001689">
        <property type="entry name" value="Flag_FliM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001543">
        <property type="entry name" value="FliN-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036429">
        <property type="entry name" value="SpoA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02154">
        <property type="entry name" value="FliM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01052">
        <property type="entry name" value="FliMN_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002888">
        <property type="entry name" value="FliM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00955">
        <property type="entry name" value="FLGMOTORFLIM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101801">
        <property type="entry name" value="SSF101801"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103039">
        <property type="entry name" value="SSF103039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01397">
        <property type="entry name" value="fliM_switch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="10" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="4 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5 10" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="12" id="KW-0969">Cilium</keyword>
    <keyword evidence="6 10" id="KW-0283">Flagellar rotation</keyword>
    <keyword evidence="12" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FliMN_C" evidence="11">
        <location>
            <begin position="308"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR002888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA04386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="401" mass="44375" checksum="CF965ADC7E634747" modified="2005-10-11" version="1">MANDSDQIDQDAIAAQWEASLDSGDPDEAAKSAAANELTGAMAEQWAAMVDDGGRDFGNGKNSGERVLSQEEIDSLLGFSVGEINLDDHSGIRAIIDSAMVSYERLPMLEIVFDRLVRLMTTSLRNFTSDNVEVSLDRITSVRFGDYLNSIPLPAVLSVFKAEEWENFGLAMVDSSLIYSMIDVLLGGRRGQSSLRIEGRPYTTIETNLVKRLVEVVLADAEQAFRPLSPVSFSIDRLETNPRFAAITRPANAAILVRLRIDMEDRGGTIELLLPYATIEPIRNVLLQMFMGEKFGRDPIWESHLATEIAQAQIAVDAVLYEAEIPLRQLMTLKVGDTLPLEMRPDASVAVRCGSVTLTEGRMGRVGDRVAIRVSRHLRKPSTTFAMFERADEQTKMMEAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STS1</accession>
    <name>Q3STS1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Cell division protein FtsZ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ftsZ</name>
        <name evidence="9" type="ordered locus">Nwi_1058</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer. Polymerizes to form a dynamic ring structure in a strictly GTP-dependent manner. Interacts directly with several other division proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="4">Assembles at midcell at the inner surface of the cytoplasmic membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the FtsZ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314354.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1058"/>
    <dbReference type="EnsemblBacteria" id="ABA04320">
        <property type="protein sequence ID" value="ABA04320"/>
        <property type="gene ID" value="Nwi_1058"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1058"/>
    <dbReference type="eggNOG" id="COG0206">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024865_5_1_5"/>
    <dbReference type="OMA" id="FRPQSKI"/>
    <dbReference type="OrthoDB" id="1009041at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051258">
        <property type="term" value="P:protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02201">
        <property type="entry name" value="FtsZ_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00909">
        <property type="entry name" value="FtsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000158">
        <property type="entry name" value="Cell_div_FtsZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017844">
        <property type="entry name" value="Cell_div_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020805">
        <property type="entry name" value="Cell_div_FtsZ_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045061">
        <property type="entry name" value="FtsZ/CetZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024757">
        <property type="entry name" value="FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314">
        <property type="entry name" value="PTHR30314"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12327">
        <property type="entry name" value="FtsZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00423">
        <property type="entry name" value="CELLDVISFTSZ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="SSF52490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="SSF55307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00065">
        <property type="entry name" value="ftsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03483">
        <property type="entry name" value="FtsZ_alphas_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01134">
        <property type="entry name" value="FTSZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01135">
        <property type="entry name" value="FTSZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 5 9" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0717">Septation</keyword>
    <feature type="domain" description="Tubulin" evidence="7">
        <location>
            <begin position="16"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin_C" evidence="8">
        <location>
            <begin position="210"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="354"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="437"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="491"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="24"/>
            <end position="28"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="111"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA04320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="603" mass="63512" checksum="AD9D79FE32DD4DD0" modified="2005-10-11" version="1">MTINLNVPDIHELKPRITVFGVGGAGGNAVNNMITAGLVGVDFVVANTDAQALTMSKAQRIIQMGTQVTQGLGAGSQPDVGAAAAEEVIDEIRDHLSGANMVFVTAGMGGGTGTGAAPVIAKAAREMGILTVGVVTKPFHFEGQRRMRTADSGIGELHKVVDTLLIIPNQNLFRVANEKTTFADAFAMADQVLYSGVACITDLMVKEGLINLDFADVRAVMREMGKAMMGTGEASGEKRALTAAEAAIANPLIDDSSMKGAKGLLISITGGKDLTLFEVDEAATRIREEVDQDANIIVGATFDESLDGIIRVSVVATGIDQSTIARTAATPAMKSGSATPDPRAAELSAKLREDNKRASANLAQKPAEPRPAVQPAQAQVQAPVQAAQPAANVDRAALEAIAAAVAEPAPPPPPAAAPAPVQPASYGDVTVRPIAQKPTLFPDHDPAPREQQEPPVPDTFIPQPAERAPLRAPRMPRMEELPMPAQNEIRQARGEVEEEHPQKSRLSLLQRLANVGLGRRDQEAEPPIAGRDAGPAMAQMPPLPERRPQRSVAEQMGNDPVSEYARRPPPKGLDAHGRPSPVAPAPQGDDHLDIPAFLRRQAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STX9</accession>
    <name>Q3STX9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Aquaporin Z</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">aqpZ</name>
        <name evidence="11" type="ordered locus">Nwi_1000</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Channel that permits osmotically driven movement of water in both directions. It is involved in the osmoregulation and in the maintenance of cell turgor during volume expansion in rapidly growing cells. It mediates rapid entry or exit of water in response to abrupt changes in osmolarity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 10">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cell inner membrane</location>
            <topology evidence="2 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314300.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1000"/>
    <dbReference type="EnsemblBacteria" id="ABA04262">
        <property type="protein sequence ID" value="ABA04262"/>
        <property type="gene ID" value="Nwi_1000"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1000"/>
    <dbReference type="eggNOG" id="COG0580">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020019_3_2_5"/>
    <dbReference type="OMA" id="IFKALMY"/>
    <dbReference type="OrthoDB" id="1744995at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00333">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01146">
        <property type="entry name" value="Aquaporin_Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023743">
        <property type="entry name" value="Aquaporin_Z"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45724">
        <property type="entry name" value="PTHR45724"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="SSF81338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00861">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="5 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="85"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="161"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="205"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="NPA 1" evidence="10">
        <location>
            <begin position="64"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="NPA 2" evidence="10">
        <location>
            <begin position="186"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="site" description="Involved in tetramerization or stability of the tetramer" evidence="10">
        <location>
            <position position="21"/>
        </location>
    </feature>
    <feature type="site" description="Selectivity filter" evidence="10">
        <location>
            <position position="44"/>
        </location>
    </feature>
    <feature type="site" description="Selectivity filter" evidence="10">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <feature type="site" description="Selectivity filter" evidence="10">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <feature type="site" description="Selectivity filter" evidence="10">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA04262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="238" mass="24322" checksum="BAD1E1BA0CAE1A33" modified="2005-10-11" version="1">MDLRKYAAELIGTFWLTFMGCGSAVIAAAFPEVGIGLLGVALTFGLSVVTMAYAIGHISGCHLNPAVTLGLTAGGRFPAAQVVPYIIAQVIGAIAAAALLYVIASGAAGFDVAKGFASNGYGEHSPGHYNLTVSFITEVTMTAMFLFVIMGSTHGKAPAGFAPLAIGLALVMIHLVSIPVTNTSVNPARSTGPALFVGGWALEQLWLFWVAPLIGGVIGGVVYRGLSSEPEGVVAGKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-09-11" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STZ6</accession>
    <name>RL36_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L36</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpmJ</name>
        <name type="ordered locus">Nwi_0983</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL36 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3STZ6"/>
    <dbReference type="SMR" id="Q3STZ6"/>
    <dbReference type="STRING" id="323098.Nwi_0983"/>
    <dbReference type="EnsemblBacteria" id="ABA04245">
        <property type="protein sequence ID" value="ABA04245"/>
        <property type="gene ID" value="Nwi_0983"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0983"/>
    <dbReference type="eggNOG" id="COG0257">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135723_3_2_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00251">
        <property type="entry name" value="Ribosomal_L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000473">
        <property type="entry name" value="Ribosomal_L36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035977">
        <property type="entry name" value="Ribosomal_L36_sp"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00444">
        <property type="entry name" value="Ribosomal_L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57840">
        <property type="entry name" value="SSF57840"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01022">
        <property type="entry name" value="rpmJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00828">
        <property type="entry name" value="RIBOSOMAL_L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000302254" description="50S ribosomal protein L36">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00251"/>
        </source>
    </evidence>
    <sequence length="41" mass="5066" checksum="EDB4E2A6D0C089FD" modified="2005-10-11" version="1">MKVRNSLKSLRSRHRDNRLVRRKGRVYVINKTQRRFKARQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU31</accession>
    <name>Q3SU31_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0948</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0948"/>
    <dbReference type="EnsemblBacteria" id="ABA04210">
        <property type="protein sequence ID" value="ABA04210"/>
        <property type="gene ID" value="Nwi_0948"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0948"/>
    <dbReference type="HOGENOM" id="CLU_2602459_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="79" mass="9203" checksum="C60ABB561966BD5B" modified="2005-10-11" version="1">MGLPFVMRSRCRRSAFLSTPSPGRLVLPEQLDPFLQFPSIEIRHGRNHVDAERLKTVQLKGPSPLFFVAATRLRRWYPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU33</accession>
    <name>Q3SU33_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Maltooligosyl trehalose synthase</fullName>
            <ecNumber evidence="2">5.4.99.15</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0946</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="5.4.99.15" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314249.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0946"/>
    <dbReference type="CAZy" id="GH13">
        <property type="family name" value="Glycoside Hydrolase Family 13"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04208">
        <property type="protein sequence ID" value="ABA04208"/>
        <property type="gene ID" value="Nwi_0946"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0946"/>
    <dbReference type="eggNOG" id="COG3280">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005045_1_0_5"/>
    <dbReference type="OMA" id="WTVVEKI"/>
    <dbReference type="OrthoDB" id="217555at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047470">
        <property type="term" value="F:(1,4)-alpha-D-glucan 1-alpha-D-glucosylmutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11336">
        <property type="entry name" value="AmyAc_MTSase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="Glyco_hydro_13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012767">
        <property type="entry name" value="Trehalose_TreY"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02401">
        <property type="entry name" value="trehalose_TreY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aamy" evidence="1">
        <location>
            <begin position="28"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="948" mass="105951" checksum="FB84938D55D081A8" modified="2005-10-11" version="1">MTLDRSDQPDRKPIPRATYRLQLNKDFGFDQAAALAPYLARLGISHVYCSPYLRARPGSTHGYDIVSHTELNPELGDTAAFERMVAAFRTNGLGQILDFVPNHMGVGGADNPWWLDVLEWGPDSQFAGWFDIDWESDRRYLLGKLLVPFLGDQYGAELQSGALRLRFDAEAGSLAVWAYETHKLPISPLHYERILGDRHPDLERIGDAFAHLSTWRPHIDRRAADLKLELAESVRCNPDLAVAIEAAVDRFNGEAGDLQSWSKLDALIRDQHWRAAHFRVAADDINYRRFFNINDLAGVRMELPQLFDHAHSLVFRLLEQGVLDGIRLDHVDGLLDPKGYFSRLREKAPRPFYLVVEKILASHENLRDDWDVEGTTGYEFTNLVTGLLNDPAGQENLTRLYADFTGERASFSDIVRDSKMRIMENEMASELNVLAREAARVARSNPRTADFTNNVLQRALKEIIAVFPVYRTYVDGCGAPTPADRRDIDWAMAQARRNGAALDPSVFDFLHQLLTCDLVAGPRSGFSRVEVVRVAMRAQQYSGPVMAKGLEDTAFYRYNRMLALNEVGGHPDQFSVSISAFHHANAQRAKRTPHAMLSTATHDTKRGEDTRARLAVLSEIPDDWAQHVALWSRMLRARNAGNADEVPPDRNDEYAFYQLLLGVWPARLSAGQLDPGEVDAFRQRIEGAMTKAMREAKVHTTWAAPNAAYEDAVLEFIGYALDTSRTNPFLESFSAFQQRVATLGALNSLIQVVLKLTAPGVPDIYQGTELWDFSMVDPDNRRAVDYDTRRTSLASLESGEGDLAELLENWRDGRLKLRLIAETLALRRREPELFVDGSYEALPATGPQADRLCAFMRAGEHSALVTAVALYPSRGHQAETWGDCNLALPSEAAPQRWTELFSGRELTLSQGSLLARDLFASLPVALLTPSASDSTRSGKALAAEPVIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU41</accession>
    <name>Q3SU41_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Flavin reductase-like, FMN-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0938</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314241.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0938"/>
    <dbReference type="EnsemblBacteria" id="ABA04200">
        <property type="protein sequence ID" value="ABA04200"/>
        <property type="gene ID" value="Nwi_0938"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0938"/>
    <dbReference type="eggNOG" id="COG1853">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059021_1_0_5"/>
    <dbReference type="OMA" id="RFAMKGR"/>
    <dbReference type="OrthoDB" id="1681849at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016646">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002563">
        <property type="entry name" value="Flavin_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01613">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00903">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavin_Reduct" evidence="2">
        <location>
            <begin position="33"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="180" mass="19245" checksum="0A183EE5ED89050A" modified="2005-10-11" version="1">MTDGSELPDTPDPARELASGESTLDPRDFRNALGTYATGVTIITAVDEEGTPHGLTCNSFASVSLNPPLVLWSLVIYSRNMSVFQNASHFAVNVLGASQQGLATKFAQPSSRKFEGVGWKPGLGGAPLLAGSVASFECRAVDRYYGGDHVIFLGAVEAYSYNRDEALLFSRGKYGRFVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU55</accession>
    <name>Q3SU55_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ornithine decarboxylase</fullName>
            <ecNumber evidence="5">4.1.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0924</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Orn/Lys/Arg decarboxylase class-II family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.17" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314227.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0924"/>
    <dbReference type="EnsemblBacteria" id="ABA04186">
        <property type="protein sequence ID" value="ABA04186"/>
        <property type="gene ID" value="Nwi_0924"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0924"/>
    <dbReference type="eggNOG" id="COG0019">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026444_1_3_5"/>
    <dbReference type="OMA" id="SFFVCDL"/>
    <dbReference type="OrthoDB" id="861683at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004586">
        <property type="term" value="F:ornithine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006596">
        <property type="term" value="P:polyamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022643">
        <property type="entry name" value="De-COase2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022644">
        <property type="entry name" value="De-COase2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022653">
        <property type="entry name" value="De-COase2_pyr-phos_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000183">
        <property type="entry name" value="Orn/DAP/Arg_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002433">
        <property type="entry name" value="Orn_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11482">
        <property type="entry name" value="PTHR11482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02784">
        <property type="entry name" value="Orn_Arg_deC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00278">
        <property type="entry name" value="Orn_DAP_Arg_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01179">
        <property type="entry name" value="ODADCRBXLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01182">
        <property type="entry name" value="ORNDCRBXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="SSF50621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="SSF51419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00878">
        <property type="entry name" value="ODR_DC_2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orn_Arg_deC_N" evidence="4">
        <location>
            <begin position="30"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Orn_DAP_Arg_deC" evidence="3">
        <location>
            <begin position="263"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="380" mass="41919" checksum="A0074ACFD21AC4BC" modified="2005-10-11" version="1">MTERIREFLRDRRNKGNDTEPCLVVDLDIVRDNYQAFAKALPDSRVFYAVKANPAPEVLSLLASLGSCFDTATVAEIEMALAAGATPDRISFGNTIKKERDIARAYALGIRLFAVDCHAEVEKIARAAPGAKVFCRILYDCAGAEWPLSRKFGCDPEMAVEVLDLAKRLSLEPIGISFHVGSQQRKVKAWDRALAMVSQVFRDCAERGINLTMVNMGGGFPTRYLKDVPPVVQYGRSIFRALRKHFGNQIPETIIEPGRGMVGNAGVIEAEVVLISRKSDNEESRWVYLDIGKFGGLAETMDESIRYAIRTPHDGAEMTPCVLAGPTCDSADVLYEKQPYPLPVTLEIGDKLLIEGTGAYTSTYSSVAFNGFPPLRTYHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU56</accession>
    <name>Q3SU56_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GCN5-related N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0923</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314226.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04185">
        <property type="protein sequence ID" value="ABA04185"/>
        <property type="gene ID" value="Nwi_0923"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0923"/>
    <dbReference type="eggNOG" id="COG3153">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081840_0_2_5"/>
    <dbReference type="OMA" id="AMPGPYE"/>
    <dbReference type="OrthoDB" id="1687413at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="17"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="199" mass="21030" checksum="38294446A1751936" modified="2005-10-11" version="1">MNAVLETIDTRCGISPFAIRAERASDVAAREALLDASFGTGRFARTCQRLRDGRKPAEGLALAAVAERKLIGTVRLWHVSAGGVPALALGPLAVAASQRKLGVGAALMKRALQLAQARGHGAVILLGDAPYYARFGFSAVRSANLLLPGPFERDRLLAVELREAALEGTSGLIVPTGARVSRARRHHGQEVARPMPRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU82</accession>
    <name>Q3SU82_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">FecR protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0895</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314200.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0895"/>
    <dbReference type="EnsemblBacteria" id="ABA04159">
        <property type="protein sequence ID" value="ABA04159"/>
        <property type="gene ID" value="Nwi_0895"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0895"/>
    <dbReference type="eggNOG" id="COG3712">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050192_0_1_5"/>
    <dbReference type="OMA" id="RYWVRVE"/>
    <dbReference type="OrthoDB" id="1381035at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006860">
        <property type="entry name" value="FecR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032623">
        <property type="entry name" value="FecR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012373">
        <property type="entry name" value="Ferrdict_sens_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30273">
        <property type="entry name" value="PTHR30273"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16220">
        <property type="entry name" value="DUF4880"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04773">
        <property type="entry name" value="FecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018266">
        <property type="entry name" value="FecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4880" evidence="3">
        <location>
            <begin position="13"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="FecR" evidence="2">
        <location>
            <begin position="111"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="320" mass="34542" checksum="FBEB7A229CFCCB5F" modified="2005-10-11" version="1">MSQTGPDQYRLMDEAIDLVIRLQNDPGNPVATEMVKVWRARSPEHERIWARVAKVHGASGKILTQRRKTERREKLGLTRRNLVIGGLIGLGTAGAGYSILPEMLLRGWADHITAKGEIRSVTLEDGSVVTLGPDSAIAVDFTTHFRRVQLLAGMSFFDVAAEPQRAFTVQAAELTATALGTAFDVAHDAGMLSISVDHGVVEARASGSAIAAGTKLSAGEWVTFDSATYAIERGSREAGQIASWRDNLIMAEKEAVSVLVARIGRWIPGRIVMADPFIGSQRVSGIFDLNNPKRALEAVVHPAGAHVRQVSSYLTVISLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUA7</accession>
    <name>Q3SUA7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Helix-turn-helix, Fis-type</fullName>
            <ecNumber evidence="3">2.7.7.49</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0870</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0870"/>
    <dbReference type="EnsemblBacteria" id="ABA04134">
        <property type="protein sequence ID" value="ABA04134"/>
        <property type="gene ID" value="Nwi_0870"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0870"/>
    <dbReference type="eggNOG" id="COG2801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2963">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027402_10_0_5"/>
    <dbReference type="OMA" id="MKHRSGT"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010921">
        <property type="entry name" value="Trp_repressor/repl_initiator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48295">
        <property type="entry name" value="SSF48295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="132"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="330"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="353" mass="40217" checksum="3BF484E0CAD546CE" modified="2005-10-11" version="1">MRYPASEKAEIIALVEQSHLPAKRTLDKLGIPRATFYRWYDRYRAGGIEALADHRSRPDRVWNRIPDDVRGQIIDLALELPELSPRELAVRFTDERKYFVSEASVYRLLKAHDLITSPAYVVIKAANEFKDKTTAANQLWQTDFTYLKITGWGWYYLSTVLDDFSRYIVAWRLGPTMCASDVTATLDQALAASGLDHVSVRQRPRLLSDNGSSYVADDLATWLRAKDMQHVRGAPYHPQTQGKIERWHQTLKNRILLENYYLPDDLKRQVAAFVEHYNHDRYHESIGNVTPADVYFCPPSGLLRQFRGFREGRISGSSQPIWSADETEIRAGESTGRAGREGHPPGNAPAVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUF9</accession>
    <name>Q3SUF9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF6362 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0817</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314130.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0817"/>
    <dbReference type="EnsemblBacteria" id="ABA04082">
        <property type="protein sequence ID" value="ABA04082"/>
        <property type="gene ID" value="Nwi_0817"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0817"/>
    <dbReference type="eggNOG" id="ENOG502ZC12">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132099_0_0_5"/>
    <dbReference type="OMA" id="RYACCIK"/>
    <dbReference type="OrthoDB" id="1996353at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045942">
        <property type="entry name" value="DUF6362"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19889">
        <property type="entry name" value="DUF6362"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6362" evidence="1">
        <location>
            <begin position="26"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="142" mass="16962" checksum="A22052B15BDCE914" modified="2005-10-11" version="1">MSKRLSGPLSPNEIEDRFEEAARTLRRLPDDKPQGYFNVWPAIVRTTWEIMAMERQPMKVWATPQSIDRMEECFAWLFWLEPEEARVVWLRAEGMRWKPICRRLGVSRATAWRWWATALIKISHRLHADDKPRMSKPRKQAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUK8</accession>
    <name>Q3SUK8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SURF1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0768</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SURF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314082.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0768"/>
    <dbReference type="EnsemblBacteria" id="ABA04033">
        <property type="protein sequence ID" value="ABA04033"/>
        <property type="gene ID" value="Nwi_0768"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0768"/>
    <dbReference type="eggNOG" id="COG3346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047737_4_1_5"/>
    <dbReference type="OMA" id="QYAFTWY"/>
    <dbReference type="OrthoDB" id="1908280at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd06662">
        <property type="entry name" value="SURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002994">
        <property type="entry name" value="Surf1/Shy1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045214">
        <property type="entry name" value="Surf1/Surf4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23427">
        <property type="entry name" value="PTHR23427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02104">
        <property type="entry name" value="SURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50895">
        <property type="entry name" value="SURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="250" mass="27067" checksum="81C323372DC13180" modified="2005-10-11" version="1">MSAATRRHSAGFGLGLVMMAMVAVLVGLGVWQLQRGTEKHALIAGLTERLAAEPVTLPEPADWATFSPARDEFRRVSFSAIYQGPDAMVYSSGSGLRSDVGLGTWAFLAARLSNGSTIVVNTGFVQNTMQDRSQQDIAVKPLVTGQPVAMTGYLRFPEKSGALTPSSSLDKRLWFSRDIGAMAQALGWGNVAPFYIDLEKPVPASGVPKPGPLDVHLKDDHIQYAITWFGLAAAVLVTFGFWWRSQRRAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUL9</accession>
    <name>Q3SUL9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0757</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314076.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04022">
        <property type="protein sequence ID" value="ABA04022"/>
        <property type="gene ID" value="Nwi_0757"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0757"/>
    <dbReference type="HOGENOM" id="CLU_2396674_0_0_5"/>
    <dbReference type="OMA" id="RESSWNE"/>
    <dbReference type="OrthoDB" id="2148332at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="93" mass="10669" checksum="4C464F5B8ACE01BA" modified="2005-10-11" version="1">MRYRTSQGLLIEAETSFDAIRRLRESSWNEQNLSDREYMEAVADRMFQEGVHVSTVSPDIFIAGLLRAGLLTREPDDAQPNAENPTKQEVDRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUN7</accession>
    <name>Q3SUN7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_0738</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0738"/>
    <dbReference type="EnsemblBacteria" id="ABA04004">
        <property type="protein sequence ID" value="ABA04004"/>
        <property type="gene ID" value="Nwi_0738"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0738"/>
    <dbReference type="eggNOG" id="COG1495">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098660_0_0_5"/>
    <dbReference type="OMA" id="EWGFWPG"/>
    <dbReference type="OrthoDB" id="1859420at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003752">
        <property type="entry name" value="DiS_bond_form_DsbB/BdbC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023380">
        <property type="entry name" value="DsbB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024199">
        <property type="entry name" value="Uncharacterised_DsbB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02600">
        <property type="entry name" value="DsbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF033913">
        <property type="entry name" value="S-S_format_DsbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158442">
        <property type="entry name" value="SSF158442"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="51"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA04004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="174" mass="18238" checksum="092303E4B2A1234B" modified="2005-10-11" version="1">MKPASAAGNRKTAFAAALAVAFIAAATLAGAWFFQLVLEILPCPLCLQQRYAYYFAIPFALLLAFAIAKGAPRCLIIPPLAVLALAALGNAGFGAYHAGVEWGFWPGPAECSGSVLDLGKGSLLENLDRVKVVRCDEVQWRFLGLSLAGYNALISLLMAAIAGWGIAALAGKVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUQ2</accession>
    <name>Q3SUQ2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sigma70_r2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0723</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314043.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0723"/>
    <dbReference type="EnsemblBacteria" id="ABA03989">
        <property type="protein sequence ID" value="ABA03989"/>
        <property type="gene ID" value="Nwi_0723"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0723"/>
    <dbReference type="HOGENOM" id="CLU_2771716_0_0_5"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="69" mass="7816" checksum="F926E2EB9EE9843D" modified="2005-10-11" version="1">MQETFLKLLTAKPTAPVDNVQAYIFRTANNLSIDLARRQRLMPLVDNSDDVLNRLSASDRIRNGSMILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUS9</accession>
    <name>Q3SUS9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphatidylethanolamine N-methyltransferase / phosphatidyl-N-methylethanolamine N-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.17</ecNumber>
            <ecNumber evidence="2">2.1.1.71</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0696</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.17" evidence="2"/>
    <dbReference type="EC" id="2.1.1.71" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314018.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0696"/>
    <dbReference type="EnsemblBacteria" id="ABA03962">
        <property type="protein sequence ID" value="ABA03962"/>
        <property type="gene ID" value="Nwi_0696"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0696"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_7_0_5"/>
    <dbReference type="OMA" id="GRYPWVK"/>
    <dbReference type="OrthoDB" id="1865374at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080101">
        <property type="term" value="F:phosphatidyl-N-dimethylethanolamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000773">
        <property type="term" value="F:phosphatidyl-N-methylethanolamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004608">
        <property type="term" value="F:phosphatidylethanolamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="1">
        <location>
            <begin position="58"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="229" mass="25357" checksum="A0BD878C1D5322CE" modified="2005-10-11" version="1">MTEILKLDDGEQLDFDRATVEQAYDRWAPVYDFVFGGVFSKGRKAAIQATGRVGGRVLEVGVGTGISLPQYAPHLRIFGTDISEAMLRKAKRRVKALGLKNVEDLAVMDAEKLEFPDNSFDVVMAQYVVTAVPNPEAALDEFSRVLRPGGELIILTRISADAGLRRFIERRLQPVVRPLGFRTAEFTWSRYAQWLATTGAMGLVENRPIPPLGHFSLLRIRKTAIAAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUZ7</accession>
    <name>Q3SUZ7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulatory protein, LuxR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0627</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313954.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0627"/>
    <dbReference type="EnsemblBacteria" id="ABA03894">
        <property type="protein sequence ID" value="ABA03894"/>
        <property type="gene ID" value="Nwi_0627"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0627"/>
    <dbReference type="eggNOG" id="COG2771">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072786_4_1_5"/>
    <dbReference type="OMA" id="RESLQWV"/>
    <dbReference type="OrthoDB" id="1687028at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005143">
        <property type="entry name" value="TF_LuxR_autoind-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036693">
        <property type="entry name" value="TF_LuxR_autoind-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03472">
        <property type="entry name" value="Autoind_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75516">
        <property type="entry name" value="SSF75516"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="2">
        <location>
            <begin position="173"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="241" mass="27004" checksum="3DF67FC68CAE30F8" modified="2005-10-11" version="1">MSRRTLDDTLTFIARVDKASTPAEIADAVVDVARPLGFSHVLAGIIPIPGMTAEQQISNVVLHRWPKAWSERYFTKGYLFDDPTIQRVNTSTEPFLWSELEPTYRNTPAPTRVMGEAREFNLGCGFTVPMITLNGQTAGFSLASERAEVPSILRGQLQLIAMYAFARALGQKYKPAPVNLTPREMDILQWMAEGKSDWEISVILKVSEHLVDKIARQLRAKLNATNRTQTVAVALRHNLIR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-01-24" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV02</accession>
    <name>PDXH_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyridoxine/pyridoxamine 5'-phosphate oxidase</fullName>
            <ecNumber evidence="1">1.4.3.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">PNP/PMP oxidase</fullName>
            <shortName evidence="1">PNPOx</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Pyridoxal 5'-phosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pdxH</name>
        <name type="ordered locus">Nwi_0622</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + O2 + pyridoxamine 5'-phosphate = H2O2 + NH4(+) + pyridoxal 5'-phosphate</text>
            <dbReference type="Rhea" id="RHEA:15817"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:58451"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="1.4.3.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>O2 + pyridoxine 5'-phosphate = H2O2 + pyridoxal 5'-phosphate</text>
            <dbReference type="Rhea" id="RHEA:15149"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:58589"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="1.4.3.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="1">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxamine 5'-phosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxine 5'-phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the pyridoxamine 5'-phosphate oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.5" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313949.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SV02"/>
    <dbReference type="SMR" id="Q3SV02"/>
    <dbReference type="STRING" id="323098.Nwi_0622"/>
    <dbReference type="EnsemblBacteria" id="ABA03889">
        <property type="protein sequence ID" value="ABA03889"/>
        <property type="gene ID" value="Nwi_0622"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0622"/>
    <dbReference type="eggNOG" id="COG0259">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032263_2_3_5"/>
    <dbReference type="OMA" id="PEPNAMV"/>
    <dbReference type="OrthoDB" id="1542844at2"/>
    <dbReference type="UniPathway" id="UPA01068">
        <property type="reaction ID" value="UER00304"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA01068">
        <property type="reaction ID" value="UER00305"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004733">
        <property type="term" value="F:pyridoxamine-phosphate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008615">
        <property type="term" value="P:pyridoxine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01629">
        <property type="entry name" value="PdxH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000659">
        <property type="entry name" value="Pyridox_Oxase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019740">
        <property type="entry name" value="Pyridox_Oxase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019576">
        <property type="entry name" value="Pyridoxamine_oxidase_dimer_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10851">
        <property type="entry name" value="PTHR10851"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10590">
        <property type="entry name" value="PNP_phzG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="Putative_PNPOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000190">
        <property type="entry name" value="Pyd_amn-ph_oxd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00558">
        <property type="entry name" value="pdxH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01064">
        <property type="entry name" value="PYRIDOX_OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0664">Pyridoxine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000167728" description="Pyridoxine/pyridoxamine 5'-phosphate oxidase">
        <location>
            <begin position="1"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="60"/>
            <end position="65"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="75"/>
            <end position="76"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="139"/>
            <end position="140"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="190"/>
            <end position="192"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01629"/>
        </source>
    </evidence>
    <sequence length="213" mass="24054" checksum="1D1C0E1CFDCD89A1" modified="2005-10-11" version="1">MNDTTDIKHTSQLTSGDFTEAREPFALFEAWLAEATASEPNDPDAMALATADADGLPDLRMVLMKGFDARGFVFYSHIASAKGRELAANPKAALLFHWKSLRRQVRVRGPVTPVTDAEADAYFATRPRQAQIGAWASRQSRPLESRLAFEQAIAKEAAKYAIGAVPRPPGWSGWRITPLQFEFWHDRPFRLHDRIEFRRSALDVPWTKTRLYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV04</accession>
    <name>Q3SV04_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0620</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03887">
        <property type="protein sequence ID" value="ABA03887"/>
        <property type="gene ID" value="Nwi_0620"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0620"/>
    <dbReference type="HOGENOM" id="CLU_2524186_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="84" mass="9397" checksum="94711F0E026FBECC" modified="2005-10-11" version="1">MSNFPRIPVLRSLGPYVGPRLWYFCRKYRALIGVAQRSKTGKEAHSGVLKMNRRHSPPVAAEALADAGIYRADIDPDSAKAWSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV49</accession>
    <name>Q3SV49_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">MJ0042 finger-like region protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0575</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313903.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0575"/>
    <dbReference type="EnsemblBacteria" id="ABA03842">
        <property type="protein sequence ID" value="ABA03842"/>
        <property type="gene ID" value="Nwi_0575"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0575"/>
    <dbReference type="eggNOG" id="ENOG5032ZVA">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079564_0_0_5"/>
    <dbReference type="OMA" id="HCTTSYA"/>
    <dbReference type="OrthoDB" id="1043256at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011723">
        <property type="entry name" value="Znf/thioredoxin_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13717">
        <property type="entry name" value="zinc_ribbon_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02098">
        <property type="entry name" value="MJ0042_CXXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Znf/thioredoxin_put" evidence="3">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="285" mass="31737" checksum="0713C9791AC37481" modified="2005-10-11" version="1">MHIVCPHCTTSYAVDPAKLGGKGRIVRCSRCKETWRATPDNIARVRVPTMAGADEMAGWTDPPDAGEMPSIDSPSIASDLPESPETRMPQQNLAVARTPPARQDNTPRDNTTRDNKRALSIASRFRDRLRSLFVFHRPTITLNTFCVAMAPLVLALVIWRVDIVRLLPQTATFYRLAGLEVNLRGLAFKDVTTSTETVDGKQVLVIEGMIAAQTRKPVELPRLRFSIRDAHETEIYAWNAVLEQPALKPGENIWFRTRLASPPPEGRSIDVRFFNRQDLAAGNGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV79</accession>
    <name>Q3SV79_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Glucose-1-phosphate thymidylyltransferase</fullName>
            <ecNumber evidence="4 5">2.7.7.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0545</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>alpha-D-glucose 1-phosphate + dTTP + H(+) = diphosphate + dTDP-alpha-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:15225"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37568"/>
            <dbReference type="ChEBI" id="CHEBI:57477"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="2.7.7.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the glucose-1-phosphate thymidylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.24" evidence="4 5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313873.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0545"/>
    <dbReference type="EnsemblBacteria" id="ABA03812">
        <property type="protein sequence ID" value="ABA03812"/>
        <property type="gene ID" value="Nwi_0545"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0545"/>
    <dbReference type="eggNOG" id="COG1209">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_9_0_5"/>
    <dbReference type="OMA" id="FTWLDTG"/>
    <dbReference type="OrthoDB" id="1004719at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008879">
        <property type="term" value="F:glucose-1-phosphate thymidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045226">
        <property type="term" value="P:extracellular polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02538">
        <property type="entry name" value="G1P_TT_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005907">
        <property type="entry name" value="G1P_thy_trans_s"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43532">
        <property type="entry name" value="PTHR43532"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01207">
        <property type="entry name" value="rmlA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transferase" evidence="6">
        <location>
            <begin position="2"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="291" mass="31821" checksum="2F02048ABE855026" modified="2005-10-11" version="1">MKGIILAGGTGSRLYPVTTVVSKQLLPVFDKPMIYYPLSTLMLGGIREIMIISTPQDEPLFHRLLGDGSEIGMRFAYATQESPRGLADAFIVGRDFIGSDAVALVLGDNIFYGHGLPQLLSSASSRNKGATVFGYVVNTPEQYGVVELDDTGRARSIEEKPRQPKSNIAVTGLYFYDNDVVEIAAGVKPSARGEIEITDVNNAYLERGDLYVEVLGRGFAWLDTGTHSSLVEASHFVQILEQRQGLRIACPEEIALRQGYISLQAFEKAAARNGKSSYGEYLRSVAHSYQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV88</accession>
    <name>Q3SV88_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Membrane fusion protein (MFP) family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_0536</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell inner membrane</location>
            <topology evidence="8">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313864.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0536"/>
    <dbReference type="EnsemblBacteria" id="ABA03803">
        <property type="protein sequence ID" value="ABA03803"/>
        <property type="gene ID" value="Nwi_0536"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0536"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023976_8_1_5"/>
    <dbReference type="OMA" id="EIKPGMP"/>
    <dbReference type="OrthoDB" id="1395473at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039562">
        <property type="entry name" value="MFP_biotin_lipoyl_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010129">
        <property type="entry name" value="T1SS_HlyD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13533">
        <property type="entry name" value="Biotin_lipoyl_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01843">
        <property type="entry name" value="type_I_hlyD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="Biotin_lipoyl_2" evidence="10">
        <location>
            <begin position="79"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="154"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA03803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="448" mass="49463" checksum="8DE98AD76F871235" modified="2005-10-11" version="1">MSPSQASLATVRQFQSETDAIREAAEPLVARVTLFVLSAFLVSIVAILCLTQVDRVITSLSGNIVPVGHVNVLQALDPSIIKTIDVREGEQVKTGQLLATLDATFTSADLTQAKLQIASLEAQAARDEAELKQQPLVFPDNPDSDFQKYAALQKALYEQRVAQYQAQINSFESKIRETKATIEKLRADDTRYRQREEIMAKIEQMRTTLAERGTGSQLNMYISQDARLELLRTLENAHNGLIEAQNTLGSITADRDAFKQQWFAQLSQDLVTTRNKLDEARATYEKAFKHQDLVRWTAAEPSVVLTMAKLSVGSVLKPGDPFITLMPIDTKLEAEIRISSRDVGFIRAGDPCVMKIDAFNAAEHGTAHGKVRWISEGTFTTDDDGKPVDAYYKARCSVDASAFKGVPSNFRLIPGMTLQGDINVGTRSVAMYLLGGMLKGMSEAMREP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVE8</accession>
    <name>Q3SVE8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TerB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0476</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313807.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0476"/>
    <dbReference type="EnsemblBacteria" id="ABA03743">
        <property type="protein sequence ID" value="ABA03743"/>
        <property type="gene ID" value="Nwi_0476"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0476"/>
    <dbReference type="eggNOG" id="COG4103">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111095_1_0_5"/>
    <dbReference type="OMA" id="IVEMMWE"/>
    <dbReference type="OrthoDB" id="1863696at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3680.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007791">
        <property type="entry name" value="DjlA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029024">
        <property type="entry name" value="TerB-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05099">
        <property type="entry name" value="TerB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158682">
        <property type="entry name" value="SSF158682"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TerB" evidence="1">
        <location>
            <begin position="30"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="159" mass="17935" checksum="C8F56B898B951094" modified="2005-10-11" version="1">MLDRLRHFIIDVISPEAPETRSFDETGCKLAATALFIHIISLDGAPSDVEIRTLHELIETRFGLDPGTANKLITSATLAEGEAVDLYQFTSVLMRSLNDEDRLRIVEMMWELVYADDHVSEFEENVVWRAADLLAISSRDRIKLKHRVGSRRSAPDPAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVF1</accession>
    <name>Q3SVF1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0473</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0473"/>
    <dbReference type="EnsemblBacteria" id="ABA03740">
        <property type="protein sequence ID" value="ABA03740"/>
        <property type="gene ID" value="Nwi_0473"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0473"/>
    <dbReference type="eggNOG" id="COG4223">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042789_0_0_5"/>
    <dbReference type="OMA" id="APVQSWI"/>
    <dbReference type="OrthoDB" id="1159325at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="127"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="293"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="496" mass="51574" checksum="BF7225ED819BEC67" modified="2005-10-11" version="1">MADDKHDDASQETNSRPLSTGDEETGTSEAAERDVQADAGETAGDHQSDDWPFTTPPASAADDRQDGFSQDDFSQDSGWQDQSSAANRETDRNSEAAGDAGSGGGSGDQPSGEQPVAPRSPSRASSALIAAVSGAAAAGIVAGGAWLAGWPPPSSAPPPASQVSQAEQVSQAEFSALANRLARIESTASQPAASPLDQATAERIETLDKSAASLREDLTTLREELASVRGQSDRLAAALKDGAAAPRNDGETPSDVASSDLAPSDLAAISARLAQTEQQIEQMTQSLTAEIAKRDAETARRDTESARNSEETAKSKQAAPADDGPLRQAVAATLLNVAVRQGQPYGDLLTAFRTLVSEPDTLKPLETFAESGVPSANALCRELLAIVPKLEPPPVRLSSANDIVNRLQESAVRLVRIERLDTAPAGESAGAVVARIKVAARHNDVATARKELNTLPPTDRTAAESWIAKVDARDAALETARQFASEAMAALAKPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVF6</accession>
    <name>Q3SVF6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EVE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0468</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313799.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0468"/>
    <dbReference type="EnsemblBacteria" id="ABA03735">
        <property type="protein sequence ID" value="ABA03735"/>
        <property type="gene ID" value="Nwi_0468"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0468"/>
    <dbReference type="eggNOG" id="COG2947">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041799_2_1_5"/>
    <dbReference type="OMA" id="LWDGIRN"/>
    <dbReference type="OrthoDB" id="1829674at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002740">
        <property type="entry name" value="EVE_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01878">
        <property type="entry name" value="EVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EVE" evidence="1">
        <location>
            <begin position="2"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="137" mass="15254" checksum="FFF566413B208199" modified="2005-10-11" version="1">MAYWLVKSEPSAWSWEQQVAKGAKGEPWTGVRNFTARQNLNAMKKGDLAFFYHSNEGKEIVGIAEIIREAYPDPTDKTGRFVCVDIKASKPLKTPVTIAAVKADRRLADIALVKYSRLSVQPVTPAEWKLVCEMGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVL5</accession>
    <name>Q3SVL5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0409</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313740.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0409"/>
    <dbReference type="EnsemblBacteria" id="ABA03676">
        <property type="protein sequence ID" value="ABA03676"/>
        <property type="gene ID" value="Nwi_0409"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0409"/>
    <dbReference type="eggNOG" id="COG4581">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008788_0_0_5"/>
    <dbReference type="OMA" id="KTHYAIE"/>
    <dbReference type="OrthoDB" id="30472at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="2">
        <location>
            <begin position="12"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="3">
        <location>
            <begin position="167"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="898"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1046"/>
            <end position="1085"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1124" mass="121274" checksum="2D018B64952B5A4C" modified="2005-10-11" version="1">MAFSSSIFPPGNGRALGAGVTAVLGPTNTGKTHLAIERMLAHSSGIIGLPLRLLARELYDKIVDRAGVDSVALVTGEEKIKPARPRFWVSTVEAMPRDLDVAFLAIDEIQIAADLERGHVFTDRILHRRGRDETLLLGAATMRPIVERILPGASIVTRPRLSQLEFAGDRKITRQPRRTAIVAFSADEVYAIAELIRRQRGGAAVVLGSLSPRTRNAQVEMFQSGDVDYLVATDAVGMGLNLDVDHVAFASDRKYDGYQFRRLTPAEFAQIAGRAGRATRNGTFGTTGRCAPFEPELVDALQNHTFEAIRTLQWRNSGLDFSSLDALQASLALTPSHEALTRAPIAEDLRVLDHAARDVEVRDMAQTAAAVERLWDACQIPDYRKIAPAAHAELVTTLFGFLMRQGRVPDHWFAAQIAQADRSDGDIDTLSARIAQIRTWTFVANRPDWLADPEHWRGISREAENKLSDALHQRLTERFVDRRTSVLMRRLRENTMLDTEIGKTGEVIVEGHVIGRLDGFTFAPDAAEAGSEAKALQAIAQKALAGEIEARAEKLSGAPDDQFVLTSDGTIRWTGDAVARLVAADDALHPRLRIISDERLTGAPREAVQARLDLWLKTHIEKLLGPLFELSKAEDVTGIARGIAFQLVESLGVLERSRIAAQLKDLDQSSRASLRKYGVRFGAYHIYFPLLLKPAARSLASLLWALKQDNVDLSALSSAQHLASSGRTSFPADKLLDRDAYRALGYRQCSERAVRVDILERLADLIRPALSWREGTPGEKPAGAFDGRGFVVTQAMTSLAGTAGEDFASILRALGYRMERCPAPPSATESGALADAAVEAVAAETSESAPQPAAASVVVPADEGAVSVRADTADAGFVEPEPAPDTDLSGVPAAGLEASVEVGEEPPAASPPAVEVSEGSDVEETAANEAAPDDSAPVVAGGAVEGDADSAAMTAPDASPLEADAEPATVEVWRPGGRAVDRRPRHDRNRYRRQQPKTDGPQAAPADGEGSEGAQGRERYGRSRRRQPTGALAETAPAGSESGPREGKGRPPRERFKGKGRDRDQDKGRVQGFRKGGREGRSDTGPSHRPYASSAPREREPSIDPDSPFAKLAALKDQLAGRKD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVQ6</accession>
    <name>Y368_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleoid-associated protein Nwi_0368</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">Nwi_0368</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to DNA and alters its conformation. May be involved in regulation of gene expression, nucleoid organization and DNA protection.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Nucleoid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the YbaB/EbfC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313699.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVQ6"/>
    <dbReference type="SMR" id="Q3SVQ6"/>
    <dbReference type="STRING" id="323098.Nwi_0368"/>
    <dbReference type="PRIDE" id="Q3SVQ6"/>
    <dbReference type="EnsemblBacteria" id="ABA03635">
        <property type="protein sequence ID" value="ABA03635"/>
        <property type="gene ID" value="Nwi_0368"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0368"/>
    <dbReference type="eggNOG" id="COG0718">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140930_0_1_5"/>
    <dbReference type="OMA" id="MGNMMKQ"/>
    <dbReference type="OrthoDB" id="1883809at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043590">
        <property type="term" value="C:bacterial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1310.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00274">
        <property type="entry name" value="DNA_YbaB_EbfC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036894">
        <property type="entry name" value="YbaB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004401">
        <property type="entry name" value="YbaB/EbfC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33449">
        <property type="entry name" value="PTHR33449"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02575">
        <property type="entry name" value="YbaB_DNA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004555">
        <property type="entry name" value="UCP004555"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82607">
        <property type="entry name" value="SSF82607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00103">
        <property type="entry name" value="DNA_YbaB_EbfC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000003787" description="Nucleoid-associated protein Nwi_0368">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00274"/>
        </source>
    </evidence>
    <sequence length="106" mass="11354" checksum="BB76E10B982D878A" modified="2005-10-11" version="1">MADFLGMMKQAAQFQSKMKAMQDELDQIEVDGASGGGLVTVRMTAKMEVKGISIDPSLIKPDEREILEDLVVTALADARRKAEVAMQEKMQALTGGLGLPPGLGFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVS1</accession>
    <name>Q3SVS1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA ligase (ATP)</fullName>
            <ecNumber evidence="2">6.5.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">NHEJ DNA polymerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">Nwi_0353</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313685.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0353"/>
    <dbReference type="EnsemblBacteria" id="ABA03620">
        <property type="protein sequence ID" value="ABA03620"/>
        <property type="gene ID" value="Nwi_0353"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0353"/>
    <dbReference type="eggNOG" id="COG1793">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3285">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008325_0_1_5"/>
    <dbReference type="OMA" id="YMANLGC"/>
    <dbReference type="OrthoDB" id="2061599at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014146">
        <property type="entry name" value="LigD_ligase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014144">
        <property type="entry name" value="LigD_PE_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014145">
        <property type="entry name" value="LigD_pol_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014143">
        <property type="entry name" value="NHEJ_ligase_prk"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13298">
        <property type="entry name" value="LigD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02777">
        <property type="entry name" value="LigD_PE_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02778">
        <property type="entry name" value="ligD_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02779">
        <property type="entry name" value="NHEJ_ligase_lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02776">
        <property type="entry name" value="NHEJ_ligase_prk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="8" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 16" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="12" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA_LIGASE_A3" evidence="15">
        <location>
            <begin position="356"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="226"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="580"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="237"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="597"/>
            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ABA03620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="913" mass="100262" checksum="5A654D7B1950E9A8" modified="2005-10-11" version="1">MPSRKLSTYRNKRDFSKTAEPSGGARIAPARQRRFVIQKHDATRLHYDFRLEYDGVFKSWAVTRGPSLDPAVKRLAVEVEDHPLDYGDFEGTIPKGQYGGGTVAIWDRGYWYADDPAKGLAKGDLKFALMGEKLRGEWVLVRMKNDRSGGKRTNWLLIKHRDDYARPGDGEDVLKQDRSVASNRTLEQIAEGKGKAPEPFMMTRRIAGSDAVWDSNKGVAARARARAERRPAKASAQPASTGKTTLKESRPQTARAHAIKSKMPDFAGLQLCEAATRPPSADSWVHEIKLDGYRIQMRIESGAVTLRTRKGLDWTDRFPAIAKAAAKLPDALIDGEIVALNANGDPDFPAMQAALAEGRTDALVYFAFDLLFTEGEDLRPLPLLARKDQLRKLLGRSAKSNRATIRYVEHFDVDGEQMLATARESGLEGIISKRIDAAYRAGRRGSWLKTKSRPGHEVVIGGWKTTGDKFRSLMAGVHNGDRLVYTGIVGTGYGRDTVKRIMPALKAAAASKSPFSGKNAPAGGRDVHWLKPELVAEIEFAGWTDDGMVRQAAFKGLRADKPAREVTAEMPTRAAALKLDKARPKATRPGTRAAAAARSDPTTSRQSRTSAARDRSTVMGVAISHPDKALWPDAGDKRPVTKHDLAAYMEAVGGWMIGHIRGRPCSIVRAPDGIHSETFFQRHAMPGLSDLVTQAKVSDDRKPYLQIDRVEALAAVAQIGGIELHPWNCAPDEYTVPGRLVFDLDPAPDVAFPDVIAAAREMRQRLEALKLESFCKTTGGKGLHVVTPLAIGPKDGLNWKQAKMFAQAVCEQMAADAPDKYLINMSKKQREGKIFLDYLRNDTKSTAVAPLSPRARPGATVSMPLNWAQVKTGLDPKRFTLRTAPDLLKKSKAWADYDSAARPLAAAIGRLVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVT0</accession>
    <name>Q3SVT0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphoryl transfer system, HPr</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0344</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313676.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0344"/>
    <dbReference type="EnsemblBacteria" id="ABA03611">
        <property type="protein sequence ID" value="ABA03611"/>
        <property type="gene ID" value="Nwi_0344"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0344"/>
    <dbReference type="eggNOG" id="COG1925">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136230_1_1_5"/>
    <dbReference type="OMA" id="SIMAMMM"/>
    <dbReference type="OrthoDB" id="404807at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00367">
        <property type="entry name" value="PTS-HPr_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000032">
        <property type="entry name" value="HPr-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035895">
        <property type="entry name" value="HPr-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001020">
        <property type="entry name" value="PTS_HPr_His_P_site"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00381">
        <property type="entry name" value="PTS-HPr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00107">
        <property type="entry name" value="PHOSPHOCPHPR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55594">
        <property type="entry name" value="SSF55594"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01003">
        <property type="entry name" value="PTS_HPr_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51350">
        <property type="entry name" value="PTS_HPR_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00369">
        <property type="entry name" value="PTS_HPR_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HPr" evidence="2">
        <location>
            <begin position="21"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="109" mass="11169" checksum="9C78ABEA9E55CAAF" modified="2005-10-11" version="1">MTGQPDDEAAPTVDGASAPGTISCDMPIVNKRGLHARASAKFVQMVERFEADVSVTRNGETVGGNSIMGLMMLSAGIGTTITVSATGPEAEAAIAAIADLIGNRFDEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVV1</accession>
    <name>Q3SVV1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Aconitate hydratase</fullName>
            <shortName evidence="9">Aconitase</shortName>
            <ecNumber evidence="9">4.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_0323</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>citrate = D-threo-isocitrate</text>
            <dbReference type="Rhea" id="RHEA:10336"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="EC" id="4.2.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <text evidence="9">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.3" evidence="9"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313655.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0323"/>
    <dbReference type="EnsemblBacteria" id="ABA03590">
        <property type="protein sequence ID" value="ABA03590"/>
        <property type="gene ID" value="Nwi_0323"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0323"/>
    <dbReference type="eggNOG" id="COG1048">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013476_2_1_5"/>
    <dbReference type="OMA" id="NGGIMQY"/>
    <dbReference type="OrthoDB" id="363064at2"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00718"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003994">
        <property type="term" value="F:aconitate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047780">
        <property type="term" value="F:citrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01580">
        <property type="entry name" value="AcnA_IRP_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044137">
        <property type="entry name" value="AcnA_IRP_Swivel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006249">
        <property type="entry name" value="Aconitase/IRP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11670">
        <property type="entry name" value="PTHR11670"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="SSF53732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01341">
        <property type="entry name" value="aconitase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5 9" id="KW-0408">Iron</keyword>
    <keyword evidence="6 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 9 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase" evidence="10">
        <location>
            <begin position="77"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase_C" evidence="11">
        <location>
            <begin position="699"/>
            <end position="831"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA03590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="905" mass="98477" checksum="CF1029D3B836A90D" modified="2005-10-11" version="1">MASLDSFKCLKTFKVGDKTYAYYSLPAAEKNGLKGISRLPYSMKVLLENLLRNEDGRSVKKEDIAAFAKWLRKRRLEHEIAFRPARVLMQDFTGVPAVVDLAAMRNAMQNLGGKPEKINPLVPVDLVIDHSVIVNFFGDNKAFARNVVEEYRQNRERYEFLKWGQKAFSNFAVVPPGTGICHQVNLEYLAQTVWTRKERLTIGKKTASTEVAYPDTVLGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPLSMLLPDVVGFKLKGALKEGVTATDLVLTVTQMLRKQGVVGKFVEFYGPGLDHLSVADKATIANMAPEYGATCGFFPIDAATIDYLKTSGRPPARVALVMKYAKAQGLFRSSASADPVFTEKLALDLGTVVSSLAGPKRPEGRQPLSAVADGFAGALADEYRKTDAGARFPVEGRNFDLGHGDVVIAAITSCTNTSNPSVLMAAGLLARKAAAKGLAAKPWVKTSLAPGSQVVAEYLAASGLQQDLDKVGFNLVGFGCTTCIGNSGPLAPEISKSVNDNGIVAAAVLSGNRNFEGRVSPDVQANYLASPPLVVAYALAGSVTKNLSREPLGTGTDGKPVYLKDIWPTSKEVNAFIKKYVTSRIFKKKYAAVFKGDDNWRKIQTVASETYRWNMNSTYVQNPPYFDGMKKEPEPIEDIVDARILAMFGDKITTDHISPAGAIKLTSPAGQYLSEHQVRPADFNQYGTRRGNHEVMMRGTFANIRIKNFMLRGADGAVPEGGSTRHWPDGEQMSIYDAAMKYQQENVPLVVFAGAEYGNGSSRDWAAKGTRLLGVRAVICESFERIHRSNLVGMGVLPLTFEDGVSWQSIGLTGDETVTIRGLQGDLKPRQKLSADIVSRDGSLRRVLLLCRIDTLDELDYYRNGGILHYVLRKLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWC2</accession>
    <name>Q3SWC2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Lysine--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Lysyl-tRNA synthetase</fullName>
            <shortName evidence="8">LysRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">lysS</name>
        <name evidence="9" type="ordered locus">Nwi_0151</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)</text>
            <dbReference type="Rhea" id="RHEA:20792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9696"/>
            <dbReference type="Rhea" id="RHEA-COMP:9697"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.6" evidence="8"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313488.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0151"/>
    <dbReference type="EnsemblBacteria" id="ABA03419">
        <property type="protein sequence ID" value="ABA03419"/>
        <property type="gene ID" value="Nwi_0151"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0151"/>
    <dbReference type="eggNOG" id="COG1384">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025562_2_0_5"/>
    <dbReference type="OMA" id="DWPMRWA"/>
    <dbReference type="OrthoDB" id="256927at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004824">
        <property type="term" value="F:lysine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006430">
        <property type="term" value="P:lysyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00177">
        <property type="entry name" value="Lys_tRNA_synth_class1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020751">
        <property type="entry name" value="aa-tRNA-synth_I_codon-bd_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008925">
        <property type="entry name" value="aa_tRNA-synth_I_cd-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002904">
        <property type="entry name" value="Lys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37940">
        <property type="entry name" value="PTHR37940"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01921">
        <property type="entry name" value="tRNA-synt_1f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48163">
        <property type="entry name" value="SSF48163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00467">
        <property type="entry name" value="lysS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="short sequence motif" description="'HIGH' region" evidence="8">
        <location>
            <begin position="48"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="8">
        <location>
            <begin position="297"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA03419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="543" mass="60596" checksum="6B63FEF3A25EAAB5" modified="2005-10-11" version="1">MSDLMSAGDLRALAEQSNAWPFEQAKLIVARLKKTPKDEVLFETGYGPSGLPHIGTFGEVARTTMVRHAFRVLTEDKIKTRLLAFSDDMDGLRKVPDNVPNKELLARDLGKPLTKVVDPFGTHSSFGEHNNARLRAFLDTFGFDYEFASSTAYYTSGRFDVTLLKVLENIDKVMAIMLPSLREERAASYSPFLPICPRTGVVLQVPISAHDAKAGTVSYDDPETKERVTVPVTGGRCKLQWKPDWAMRWTALGVDYEMAGKDLIDSVKLSGKICSALGGTPPEGFNYELFLDDKGQKISKSKGNGLTIDEWLRYASPESLSLFMYREPKAAKRLYFDVIPRNVDDYQQFLEGYPRQDARQQLANPVWHIHAGHPPSADMPVTFQLLLTLVSSSNAENAETLWGFIGRYRPGVTPQTHPKLDALVGYAINYYRDFVAPTKTFREPTASERAALQDLREALSQLPADASAEDIQNTVYEIGRREPFLNHKKPARDGRPGVSLDWFNMLYQVLLGQEKGPRFGSFVAVYGVQNAIAMIDGALARAD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWF2</accession>
    <name>HIS2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribosyl-ATP pyrophosphatase</fullName>
            <shortName evidence="1">PRA-PH</shortName>
            <ecNumber evidence="1">3.6.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisE</name>
        <name type="ordered locus">Nwi_0121</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + H2O = 1-(5-phospho-beta-D-ribosyl)-5'-AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22828"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="3.6.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PRA-PH family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.31" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWF2"/>
    <dbReference type="SMR" id="Q3SWF2"/>
    <dbReference type="STRING" id="323098.Nwi_0121"/>
    <dbReference type="EnsemblBacteria" id="ABA03389">
        <property type="protein sequence ID" value="ABA03389"/>
        <property type="gene ID" value="Nwi_0121"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0121"/>
    <dbReference type="eggNOG" id="COG0140">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123337_1_1_5"/>
    <dbReference type="OMA" id="FTHEKGE"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00007"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004636">
        <property type="term" value="F:phosphoribosyl-ATP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11534">
        <property type="entry name" value="NTP-PPase_HisIE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01020">
        <property type="entry name" value="HisE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008179">
        <property type="entry name" value="HisE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021130">
        <property type="entry name" value="PRib-ATP_PPHydrolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01503">
        <property type="entry name" value="PRA-PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03188">
        <property type="entry name" value="histidine_hisI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000230180" description="Phosphoribosyl-ATP pyrophosphatase">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01020"/>
        </source>
    </evidence>
    <sequence length="116" mass="12777" checksum="A9E1F33F0BF08DD8" modified="2005-10-11" version="1">MRERTSRRIMQRFTIHDLAAIIDARAASGGDASYTRKLLDKGPAHCARKLGEEAVETIIAAVEKDRHHLIAEGADLMFHFLVLLKASGVTLQDVEAVLAQRTAISGLEEKAARNRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWF9</accession>
    <name>Q3SWF9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_0114</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313451.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0114"/>
    <dbReference type="EnsemblBacteria" id="ABA03382">
        <property type="protein sequence ID" value="ABA03382"/>
        <property type="gene ID" value="Nwi_0114"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0114"/>
    <dbReference type="eggNOG" id="COG0784">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2202">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_51_5"/>
    <dbReference type="OMA" id="CNTDISA"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAC" evidence="8">
        <location>
            <begin position="248"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="302"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="8">
        <location>
            <begin position="375"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="439"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="5">
        <location>
            <begin position="578"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="6">
        <location>
            <begin position="822"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="4">
        <location>
            <position position="872"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA03382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="939" mass="104689" checksum="A9D36EF26BAB7CE8" modified="2005-10-11" version="1">MPEKESVSSGAFLAGGGEMGELTRRFDWDSTSVGTPDAWPQSLRTAVRIVLNTNHPMFIWWGSDLIQFYNDAYRQTLGPERHPGALGQKGRECWAEIWDIIGPQIEYVMSGRGATWHEEQLVPVTRNGRLEQVYWTYGFSPIDDEEGVGGVLVVCRDVTREHLARMALQERETELARVQAIGRIGGLEVDLRTGHRSRKSPEYLKIHGLPSDAVHESREDWLRRVHPDDRAVAEGQFIDAIRGGDREYAVRYRIIRPNDGETRWISARSVIERDADGKAIRLFGAHIDITDQVEAERSIRQREQEFRTLAEALPHHVWTATSDGSFNWFNRRFYDYVGARPGEPGGNDWNRIVHPDDAPAAAAARTRAVTSGEPYEIEFRLRRSDGVYHWFLSRALPARDEDGRIIRWIGTDTDVHDQKRIAAKLAELNATLAERVEEKTRERDRIWNVSQDLLLVMDQHGIWRSVNPAWTRTLGWSEAELTERTAEWIEHPDDIVKTRVEVRRLARGKATVRFENRLRHKDGSYRWLSWTAVPDQNLIYAVARDVTAEKAAAERLRAAEEALRQSQKMEAVGQLTGGIAHDFNNLLTGIVGSLDLLQTHIKQGRTDRAGRYIEAAMASANRAAALTHRLLAFARRQPLVPKPVDANQLILSLEDLLRRTIGEAIELKIAPSKLLWPTLCDPNQLESALLNLAINARDAMPNGGRLTIATANVSLDTAGSGSSTRQPGDYICITVADTGTGMTADVAARAFDPFFTTKPIGHGTGLGLSMIYGFARQSNGHIALDTAPGRGTSISLSLPRHDSVAEAVQASPASRITATGGTVLVVEDEPVVRGIIVEMLHDQGYVTREAADGAEGLRILQLDKPVDLLLTDIGLPGMNGRQLADQARELRPDLKILFMTGYADNAANAKGFLQPGMDMITKPFDLGHLSRRVRDIISH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWG5</accession>
    <name>Q3SWG5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidoglycan lytic exotransglycosylase</fullName>
            <ecNumber evidence="2">4.2.2.n1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Murein hydrolase A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_0108</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exolytic cleavage of the (1-&gt;4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan, from either the reducing or the non-reducing ends of the peptidoglycan chains, with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.</text>
            <dbReference type="EC" id="4.2.2.n1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="4.2.2.n1" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0108"/>
    <dbReference type="CAZy" id="GH102">
        <property type="family name" value="Glycoside Hydrolase Family 102"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03376">
        <property type="protein sequence ID" value="ABA03376"/>
        <property type="gene ID" value="Nwi_0108"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0108"/>
    <dbReference type="eggNOG" id="COG2821">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037751_0_0_5"/>
    <dbReference type="OMA" id="MQRIREW"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019867">
        <property type="term" value="C:outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009254">
        <property type="term" value="P:peptidoglycan turnover"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14485">
        <property type="entry name" value="mltA_like_LT_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010611">
        <property type="entry name" value="3D_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026044">
        <property type="entry name" value="MltA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034654">
        <property type="entry name" value="MltA_3D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005300">
        <property type="entry name" value="MltA_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30124">
        <property type="entry name" value="PTHR30124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06725">
        <property type="entry name" value="3D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03562">
        <property type="entry name" value="MltA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00925">
        <property type="entry name" value="MltA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="SSF50685"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MltA" evidence="7">
        <location>
            <begin position="145"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="417"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="440"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="483"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA03376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="502" mass="55227" checksum="C6D2DF0CE0D0EBEE" modified="2005-10-11" version="1">MAPEAPAELYRVRRFAVGLCIAAATAPVTVQAQGSGHRSVGPLEITGAQYSPVAFADIAGWNTDNHLEAFKAFRASCRGVTARRVSAVSHKALGASLREPCRVAKPMNISDNAKARAFFEDHFRAVRISRLGETDGFVTGYYEPIIDGSRTRTDVYTVPVYRRPSNLFVRGFSQSAPSLPNSGPVFRKIGRRKLVPYYDRAEIEDGAIEGRGLEICWLKSQTDLLFTQIQGSARVRLEDGSTIRINYDSYNGYPYTAVGRILIERDIIPKDQMSMQRIRQWMADNPDGANELRRQNRSYVFFHEVKLSDNDEAIGAQGLPLTPGRSIAVDKSLHVYGTPFFIEGELPIESAQAKTPFHRLMIAQDTGSAIVGPARADLYFGAGSEAGRVSGRLRHNMNFVMLVPNSLDPVARARTMPLPETRPSATIARLFPPSDQKPGSTPPASGLSSTPAREQTGAAAVPTAKAASSEAAPLPSARPGVRSSRKPRYRKHQRRLWHRRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWJ2</accession>
    <name>Q3SWJ2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ISSpo8, transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0081</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313418.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0081"/>
    <dbReference type="EnsemblBacteria" id="ABA03349">
        <property type="protein sequence ID" value="ABA03349"/>
        <property type="gene ID" value="Nwi_0081"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0081"/>
    <dbReference type="eggNOG" id="COG3677">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044348_1_2_5"/>
    <dbReference type="OMA" id="RIKMATI"/>
    <dbReference type="OrthoDB" id="1667004at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024445">
        <property type="entry name" value="Tnp_ISXO2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024442">
        <property type="entry name" value="Transposase_Zn_ribbon"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12762">
        <property type="entry name" value="DDE_Tnp_IS1595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12760">
        <property type="entry name" value="Zn_Tnp_IS1595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01126">
        <property type="entry name" value="DDE_Tnp_IS1595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE_Tnp_IS1595" evidence="1">
        <location>
            <begin position="132"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="323" mass="36622" checksum="1C2B1F88F0F2F1F3" modified="2005-10-11" version="1">MTDLLKNPIFQDETKARDWLEARVWANGRVCPHCGNADQEKITKLEGKAHRPGVYQCNEPACREQFTVTVGTVFERSKIPLTKWLAALFLMTASKKGVSAHQVHRMLGISYKSTWFLMHRLREAMRTGGLEPLGGEGKIVEADETYFGKADTQRVSPQRKGRTFTKNRKIKNDRPIVSLVERGGNVRSFHVAVADSSTVACIVNANVHKESRLQTDESRLYTKVGADFAAHETVNHGAKEYARGDVTTNTIESYFSVFKRGMRGTYQHCSEKHLHRYLAEFDFRYNNRIALGVNDTDRANELAKGIVGKRLTYRRPNSKDVQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWN1</accession>
    <name>Q3SWN1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">2-octaprenylphenol hydroxylase</fullName>
            <ecNumber evidence="10">1.14.13.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_0042</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; ubiquinone biosynthesis [regulation].</text>
    </comment>
    <dbReference type="EC" id="1.14.13.-" evidence="10"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313381.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0042"/>
    <dbReference type="EnsemblBacteria" id="ABA03310">
        <property type="protein sequence ID" value="ABA03310"/>
        <property type="gene ID" value="Nwi_0042"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0042"/>
    <dbReference type="eggNOG" id="COG0661">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006533_0_0_5"/>
    <dbReference type="OMA" id="RRDYKRV"/>
    <dbReference type="OrthoDB" id="678616at2"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010232">
        <property type="entry name" value="UbiB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01982">
        <property type="entry name" value="UbiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="502"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="122"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA03310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="526" mass="57899" checksum="EB4B1808B41F0894" modified="2005-10-11" version="1">MISALGHVARLARAAFVFAREGVFGVVDPSLVPAPGQLALRMARLIERRGAKSGSRLTRALTRLGPAYLKLGQFLATRPDVIGVAMARDLESLQDRLPPFSQAEAEAVVAAALEKPVADAFANFGPSVAAASIAQVHRGEVEKNGVRRQVAIKVLRPNVATRFRRDLSDFFFVANNAEAHSAEARRLRLVEVINTMSRSVAMEMDLRLEAAALSEMAENTRNDPDFRVPEVDWDRTTHNVLTMEWIDGIALNDHARLAQSKADLPELGRKVIQSFLRHALRDGFFHADMHPGNLFLDGSGRLVAVDFGIMGRLGLKERRFLAEILLGFITRDYRRVAEVHFEAGYVPGHHSVENFAQAIRAIGEPIHNRTAEEISMAKLLTLLLEVTGLFDMQTRPELILLQKTMVVVEGVARSFDPKLDIWTVADPVVREWITENLGPLGRMQGAIAGAGELGRLLASLPAIASRSVAVLEQVETMTRDGLVLAPETVATLACARARQNRWRTIALWIIAVTLLGILWAVSSRLI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWQ3</accession>
    <name>TRMB_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">tRNA (guanine-N(7)-)-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">tRNA (guanine(46)-N(7))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">tRNA(m7G46)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">trmB</name>
        <name type="ordered locus">Nwi_0020</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)-methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42708"/>
            <dbReference type="Rhea" id="RHEA-COMP:10188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10189"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74480"/>
            <dbReference type="EC" id="2.1.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">tRNA modification; N(7)-methylguanine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.33" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313359.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWQ3"/>
    <dbReference type="SMR" id="Q3SWQ3"/>
    <dbReference type="STRING" id="323098.Nwi_0020"/>
    <dbReference type="EnsemblBacteria" id="ABA03288">
        <property type="protein sequence ID" value="ABA03288"/>
        <property type="gene ID" value="Nwi_0020"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0020"/>
    <dbReference type="eggNOG" id="COG0220">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050910_0_3_5"/>
    <dbReference type="OMA" id="PDPWHKS"/>
    <dbReference type="OrthoDB" id="1025521at2"/>
    <dbReference type="UniPathway" id="UPA00989"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008176">
        <property type="term" value="F:tRNA (guanine-N7-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01057">
        <property type="entry name" value="tRNA_methyltr_TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003358">
        <property type="entry name" value="tRNA_(Gua-N-7)_MeTrfase_Trmb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23417">
        <property type="entry name" value="PTHR23417"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02390">
        <property type="entry name" value="Methyltransf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00091">
        <property type="entry name" value="TIGR00091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51625">
        <property type="entry name" value="SAM_MT_TRMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000229178" description="tRNA (guanine-N(7)-)-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="257" mass="29020" checksum="A37A15B209D03334" modified="2005-10-11" version="1">MTVVVSDHQNPRPPGDDAAPLGRTGNRDRPPGSFFGRRKGHRLRPHQNDLVAQLLPRLGFDPARIGPSGPAALFDPPVDETRIEIGFGGGEHLVAEALAFPRAGFIGCEPYVNGMAKILVQIEAHAIRNIRLFAGDASQLLACLPPASLARIDLIHPDPWPKRRHWKRRFVQDATVAEMARVLRPEGEFRFVSDIDDYCAWTLTHLARSPDFLWLAERSADWQDPWSGYTMTRYGRKAMREGRRAAYLRFRRERTAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMW2</accession>
    <name>Q3SMW2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_3132</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3132"/>
    <dbReference type="EnsemblBacteria" id="ABA06379">
        <property type="protein sequence ID" value="ABA06379"/>
        <property type="gene ID" value="Nwi_3132"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3132"/>
    <dbReference type="eggNOG" id="COG3019">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112034_0_0_5"/>
    <dbReference type="OMA" id="ISCHTAE"/>
    <dbReference type="OrthoDB" id="1676023at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007332">
        <property type="entry name" value="DUF411"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04214">
        <property type="entry name" value="DUF411"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="124" mass="12901" checksum="67CEF8F73BFDDFC1" modified="2005-10-11" version="1">MTVTRDPNCGCCGGWVAHVKAAGFPAEVVEAADLTPLKTRLGVPDALISCHTAEVGSYVVEGHVPAEAIKLVKRPQATGLAVAGMPIGSPGMEVSGTLPETYEVVLFGQGQQRVFAHYRGSQAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMW6</accession>
    <name>Q3SMW6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_3128</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316283.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3128"/>
    <dbReference type="EnsemblBacteria" id="ABA06375">
        <property type="protein sequence ID" value="ABA06375"/>
        <property type="gene ID" value="Nwi_3128"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3128"/>
    <dbReference type="HOGENOM" id="CLU_2260805_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="103" mass="10930" checksum="F28B692AD29FFA90" modified="2005-10-11" version="1">MKARLESVGVTEKHLAIQIAINGQDFWQSGTAGFSCGQQGISSGIDAMEISEAAATTAPLTDPVNGPAISPTIARIGSNLRSQMPTLMIGKWHRLRVLGRRGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMZ1</accession>
    <name>Q3SMZ1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_3103</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06350">
        <property type="protein sequence ID" value="ABA06350"/>
        <property type="gene ID" value="Nwi_3103"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3103"/>
    <dbReference type="eggNOG" id="ENOG5033BJV">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1600974_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="166" mass="17772" checksum="A78DC558C1318C3E" modified="2005-10-11" version="1">MVDVTYYVALPVIAAEYSIAPGEAAECQSSHAAIRKAEQLSRVSGNVGAVAFSRTGDPAVTEFSEAVVLKAFAEASDLSALSTRSSFTKRRPFLTAVEDLLPSHPFVAPPVTSPLMFAEDDMRVGAPAIVVNDDPGVRRVAVMIRCVPAHVFGTNDPRRSRQGRKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN16</accession>
    <name>Q3SN16_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_3077</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316240.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3077"/>
    <dbReference type="EnsemblBacteria" id="ABA06325">
        <property type="protein sequence ID" value="ABA06325"/>
        <property type="gene ID" value="Nwi_3077"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3077"/>
    <dbReference type="eggNOG" id="COG4191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040271_0_0_5"/>
    <dbReference type="OMA" id="EILIYVP"/>
    <dbReference type="OrthoDB" id="1827824at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004673">
        <property type="term" value="F:protein histidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="177"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="267"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA06325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="492" mass="53635" checksum="011430EAA06AE834" modified="2005-10-11" version="1">MSQAIEQLRIRAARLSLRGRLGLSGKLLLLTIPLVMIAEVLIYVPSIANFRVNRLNDRLAAANTAALVLDAAPSGMVPESLARQILGSIGARAVAIKMGQQRRLLASADLPASIDHDIDMRTMTPWSAIVGSFETMLESGNQAIRIIGPGPRGAQFIEVVTDEEPLRQSMYRFSRNLLLVSLAIAVLTAALVYLALHYLFVRPMRRLTANLVGFHQNPESPARIIVPSQRGDEIGVAERELSDMQRDLVSMLHQKSRLAALGLAVSKINHDLRNLLASSQLLSDQLASVPDPRVQRFAPKLMRSLERAIAFCQSTLSYGRAQEATPDRRLMAIEPIVNEVRESAGLTPEGSITWIDAIERGLTIDADPDQLFRILLNLVRNAAQALETQPRNEAATRQIRVAGRREGAVAIIEVSDTGPGVPEQARSHLFEAFQSSGRPGGSGLGLAIAAELVRAHGGDIHLVEGTIGATFRITIPDRPVELQTIRNERARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN74</accession>
    <name>Q3SN74_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ErfK/YbiS/YcfS/YnhG</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_3017</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YkuD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316185.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3017"/>
    <dbReference type="MEROPS" id="C82.003"/>
    <dbReference type="EnsemblBacteria" id="ABA06267">
        <property type="protein sequence ID" value="ABA06267"/>
        <property type="gene ID" value="Nwi_3017"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3017"/>
    <dbReference type="eggNOG" id="COG1376">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042399_2_1_5"/>
    <dbReference type="OMA" id="FRMTNED"/>
    <dbReference type="OrthoDB" id="975147at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071972">
        <property type="term" value="F:peptidoglycan L,D-transpeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16913">
        <property type="entry name" value="YkuD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005490">
        <property type="entry name" value="LD_TPept_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038063">
        <property type="entry name" value="Transpep_catalytic_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03734">
        <property type="entry name" value="YkuD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141523">
        <property type="entry name" value="SSF141523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229164" evidence="4">
        <location>
            <begin position="23"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="YkuD" evidence="5">
        <location>
            <begin position="59"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA06267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="187" mass="20705" checksum="4F3818D3041214DA" modified="2005-10-11" version="1">MTRVLALLIATAFVFSAKPALAQFLDTGDFMNFGSGYTSASPIPRQLVYFQSRYAPGTVVIRTGERRLYLVLPGGQALRYGIGVGRDGFRWSGVHRISAKKEWPSWTPPAQMLRRRPDLPRFMRGGLENPLGARAMYLGSTLYRIHGSNEPETIGQAVSSGCFRMTNDDVVDLYNRVSVGTTVVVKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNF6</accession>
    <name>Q3SNF6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2935</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316110.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06185">
        <property type="protein sequence ID" value="ABA06185"/>
        <property type="gene ID" value="Nwi_2935"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2935"/>
    <dbReference type="eggNOG" id="ENOG5033AXH">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1451847_0_0_5"/>
    <dbReference type="OMA" id="WASDMYA"/>
    <dbReference type="OrthoDB" id="1666077at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="171" mass="19397" checksum="757264342D60B930" modified="2005-10-11" version="1">MKRAVRDQVASMQFRPDAHHRSVQAVLKDPKLSSDEKRAILSVWASDMYAVESSPMLRMIPGHTTPLRLSDILSALRGLDEDDPPPRGGAAKRIMAGNIDADSHATIAPAVYHLARLRREADLRAASMRAHRNNIRRYQRILKTDLTELERQFVVRRLAQERSAIRNLAQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNI3</accession>
    <name>Q3SNI3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2908</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06158">
        <property type="protein sequence ID" value="ABA06158"/>
        <property type="gene ID" value="Nwi_2908"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2908"/>
    <dbReference type="HOGENOM" id="CLU_189913_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="64" mass="6514" checksum="B512079766181E6C" modified="2005-10-11" version="1">MSAIGTMNMAGVVAHMTKLRRATVGILGADVDGVLLDGVAGLVVQMAVMQVVDMIAVFDPDVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNX0</accession>
    <name>Q3SNX0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2768</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315966.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06021">
        <property type="protein sequence ID" value="ABA06021"/>
        <property type="gene ID" value="Nwi_2768"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2768"/>
    <dbReference type="eggNOG" id="ENOG5030VJP">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1833079_0_0_5"/>
    <dbReference type="OMA" id="VKESTGH"/>
    <dbReference type="OrthoDB" id="1786778at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="140" mass="14670" checksum="E4C2C362E4C5BB82" modified="2005-10-11" version="1">MFQRAIDDFKESAGSAVQLTGLALAAAFAAFVTIVFLCAAAFVFVLQNYGLIEACLSGAAIFSLITFIAVVTLLIRRKTAKKRAVQHAKTATQSFLADPMVVAAGLQLVRAVGVKKLIPIVAIGGLALGLFAGGRSRKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNZ8</accession>
    <name>Q3SNZ8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Inositol-1-monophosphatase</fullName>
            <ecNumber evidence="6">3.1.3.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2740</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>a myo-inositol phosphate + H2O = myo-inositol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24056"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:84139"/>
            <dbReference type="EC" id="3.1.3.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.25" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315938.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2740"/>
    <dbReference type="EnsemblBacteria" id="ABA05993">
        <property type="protein sequence ID" value="ABA05993"/>
        <property type="gene ID" value="Nwi_2740"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2740"/>
    <dbReference type="eggNOG" id="COG0483">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044118_0_0_5"/>
    <dbReference type="OMA" id="RVDGYWE"/>
    <dbReference type="OrthoDB" id="1741160at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008934">
        <property type="term" value="F:inositol monophosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052832">
        <property type="term" value="F:inositol monophosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052833">
        <property type="term" value="F:inositol monophosphate 4-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046855">
        <property type="term" value="P:inositol phosphate dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01639">
        <property type="entry name" value="IMPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033942">
        <property type="entry name" value="IMPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020583">
        <property type="entry name" value="Inositol_monoP_metal-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020550">
        <property type="entry name" value="Inositol_monophosphatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022337">
        <property type="entry name" value="Inositol_monophosphatase_SuhB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01959">
        <property type="entry name" value="SBIMPHPHTASE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00629">
        <property type="entry name" value="IMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00630">
        <property type="entry name" value="IMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="263" mass="28582" checksum="C4ACDB67DBE2C8F7" modified="2005-10-11" version="1">MLQSALMNVMVKAARRAGRSLKRDLGEIENLQVSLKGPANFVSLADKRAEDILYDELNKARPGYGFFGEEGGSREGTDKSHTWIVDPLDGTTNFLHGIPHFAISIGLQRDGVLIAGLVYNPANDELYTAERGKGAFLNDQRLRVSGRRQMNDCVIACGLPHIGRGDHELSRREMAALQPKVAGLRRFGAASLDLAFVAAGRVDGYWERNLQPWDIAGGQIIVREAGGIVSGIQGDDDPLTTGHVICGNEFVHAELVKIIKPLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP19</accession>
    <name>Q3SP19_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cell division and transport-associated protein TolR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_2719</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the ExbD/TolR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315918.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2719"/>
    <dbReference type="EnsemblBacteria" id="ABA05972">
        <property type="protein sequence ID" value="ABA05972"/>
        <property type="gene ID" value="Nwi_2719"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2719"/>
    <dbReference type="eggNOG" id="COG0848">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085305_1_3_5"/>
    <dbReference type="OMA" id="MMTHGVK"/>
    <dbReference type="OrthoDB" id="1940365at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003400">
        <property type="entry name" value="ExbD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014168">
        <property type="entry name" value="Tol-Pal_TolR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30558">
        <property type="entry name" value="PTHR30558"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02472">
        <property type="entry name" value="ExbD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02801">
        <property type="entry name" value="tolR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="151" mass="16272" checksum="6CD1CE9EDB283CC8" modified="2005-10-11" version="1">MAMNSAAPSSGGGGRRGRRRSQPMAEINVTPMVDVMLVLLIIFMVSAPLLTVGVPLDLPQTQAKSLEQDKTPLQLSVDAQGRIFINDTEVTMEELIPKLTAITDARGGLDERIFMRADKKADYGTVARVMGRLSGAGFKRLALVTEVEQGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP27</accession>
    <name>Q3SP27_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA(Ile)-lysidine synthase</fullName>
            <ecNumber evidence="6">6.3.4.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">tRNA(Ile)-2-lysyl-cytidine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">tRNA(Ile)-lysidine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tilS</name>
        <name evidence="9" type="ordered locus">Nwi_2711</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>ATP + cytidine(34) in tRNA(Ile2) + L-lysine = AMP + diphosphate + H(+) + lysidine(34) in tRNA(Ile2)</text>
            <dbReference type="Rhea" id="RHEA:43744"/>
            <dbReference type="Rhea" id="RHEA-COMP:10625"/>
            <dbReference type="Rhea" id="RHEA-COMP:10670"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:83665"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.19"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the tRNA(Ile)-lysidine synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.19" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2711"/>
    <dbReference type="EnsemblBacteria" id="ABA05964">
        <property type="protein sequence ID" value="ABA05964"/>
        <property type="gene ID" value="Nwi_2711"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2711"/>
    <dbReference type="eggNOG" id="COG0037">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018869_3_2_5"/>
    <dbReference type="OMA" id="PWQDPHN"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01992">
        <property type="entry name" value="PP-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01161">
        <property type="entry name" value="tRNA_Ile_lys_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012094">
        <property type="entry name" value="tRNA_Ile_lys_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012795">
        <property type="entry name" value="tRNA_Ile_lys_synt_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43033">
        <property type="entry name" value="PTHR43033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02432">
        <property type="entry name" value="lysidine_TilS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="ATP_bind_3" evidence="8">
        <location>
            <begin position="40"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="345"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="45"/>
            <end position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="371" mass="40454" checksum="A0C7D84B26EA890A" modified="2005-10-11" version="1">MARTRSSARATKPLKACTGSPIADTETKRLFSDWKASRALVLAVSGGPDSVALMWLAARWRRAMKRGPDLVAVTVDHALRREAAREARQVKQLAKSLSIPHRTLRWTGPKPATGLPAAAREARYALLFKAARSLGASHVLTGHTLDDQAETVLMRISRGSGIAGLSAMSRETMRDGFVLVRPLLNVPKARLIATLKKAKIDYAEDPTNRDLGFTRPRLRALMPALAEEGCDARNLSRLASRLMRANAALELLVDGAERFLALQDTAAPRPGFRTEAFMVLSEEIKVRLLQRTIDRFGCEGPAELGKVETLLSALESAANDADSGRRILLRRTLAGALVTLTSERISIEPAPPRRGSKGETDQGGGEREYRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP40</accession>
    <name>Q3SP40_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Abasic site processing protein</fullName>
            <ecNumber evidence="7">3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_2698</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the SOS response-associated peptidase family.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315897.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2698"/>
    <dbReference type="EnsemblBacteria" id="ABA05951">
        <property type="protein sequence ID" value="ABA05951"/>
        <property type="gene ID" value="Nwi_2698"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2698"/>
    <dbReference type="eggNOG" id="COG2135">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035990_6_2_5"/>
    <dbReference type="OMA" id="FPARYNI"/>
    <dbReference type="OrthoDB" id="1718602at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:cellular response to DNA damage stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018142">
        <property type="term" value="P:protein-DNA covalent cross-linking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1680.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003738">
        <property type="entry name" value="SRAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036590">
        <property type="entry name" value="SRAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13604">
        <property type="entry name" value="PTHR13604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02586">
        <property type="entry name" value="SRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143081">
        <property type="entry name" value="SSF143081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0190">Covalent protein-DNA linkage</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="225"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="255" mass="28216" checksum="A75FF85852C25FCC" modified="2005-10-11" version="1">MCGRYVILSPPEAMRQAFGYAEQPNFPPRYNVAPTQPVPVILLENGGRHFRLMRWGLIPVWVKDPRQFALLINARAETVLDKPAFRNAMKRRRCLLPADGYYEWRQSEGRKQPLFIRPGHGGLMAFAGLAETWNGPNGEELDTVAIITTAARGDIATLHPRVPVTIAPRDHARWLDGNAVDAGGATLLLRAPENGEFVWHEVSVRVNRVVNDDEQLIMPISAEQAESEAKQAARKPVARKVAPAASGNDGQGSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP46</accession>
    <name>Q3SP46_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Fructose-1,6-bisphosphate aldolase</fullName>
            <shortName evidence="10">FBP aldolase</shortName>
            <ecNumber evidence="10">4.1.2.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_2692</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 10">Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:14729"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.1.2.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 2 Zn(2+) ions per subunit. One is catalytic and the other provides a structural contribution.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10">One is catalytic and the other provides a structural contribution.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Carbohydrate biosynthesis; Calvin cycle.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 10">Belongs to the class II fructose-bisphosphate aldolase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.13" evidence="10"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315891.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2692"/>
    <dbReference type="EnsemblBacteria" id="ABA05945">
        <property type="protein sequence ID" value="ABA05945"/>
        <property type="gene ID" value="Nwi_2692"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2692"/>
    <dbReference type="eggNOG" id="COG0191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040088_0_0_5"/>
    <dbReference type="OMA" id="NNMEIVQ"/>
    <dbReference type="OrthoDB" id="827430at2"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00183"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00116"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019253">
        <property type="term" value="P:reductive pentose-phosphate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00947">
        <property type="entry name" value="TBP_aldolase_IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000771">
        <property type="entry name" value="FBA_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006412">
        <property type="entry name" value="Fruct_bisP_Calv"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01116">
        <property type="entry name" value="F_bP_aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001359">
        <property type="entry name" value="F_bP_aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00167">
        <property type="entry name" value="cbbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01521">
        <property type="entry name" value="FruBisAldo_II_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00602">
        <property type="entry name" value="ALDOLASE_CLASS_II_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00806">
        <property type="entry name" value="ALDOLASE_CLASS_II_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0113">Calvin cycle</keyword>
    <keyword evidence="10" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Dihydroxyacetone phosphate binding" evidence="8">
        <location>
            <begin position="233"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Dihydroxyacetone phosphate binding" evidence="8">
        <location>
            <begin position="275"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001359-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001359-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001359-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA05945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="361" mass="39422" checksum="6AB8FE5C41044E2E" modified="2005-10-11" version="1">MARITLRQLLDHAAEHGYGVPAFNINNMEQGLAIMEAAAAVDAPVILQASRGARSYANDIMLANMIDALDQMYPHIPLCMHQDHGNEEATCATAIKYGFTSVMMDGSLKADAKSAADYEYNVDITRRVVDMAHWVGASVEGELGVLGSLEHGGGEQEDGHGIEGPVSHDQLLTDPDQAVDFVRATKVDALAIAMGTSHGAYKFSRKPDGDILAMKVVEEIHRRLPNTHLVMHGSSSVPQHLQDEFNKYGGEMPQTWGVPVEEIVRGIKHGVRKVNIDTDCRLAMTAVFRKVAASNKSEFDPRKFLKPAMDAMRDLCRERFEQFGTAGNASKIKVIPLAEMAKLYRAGKLDPRIGETAVAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPB8</accession>
    <name>Q3SPB8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 9">6-carboxy-5,6,7,8-tetrahydropterin synthase</fullName>
            <ecNumber evidence="9">4.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_2620</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the conversion of 7,8-dihydroneopterin triphosphate (H2NTP) to 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) and acetaldehyde.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>7,8-dihydroneopterin 3'-triphosphate + H2O = 6-carboxy-5,6,7,8-tetrahydropterin + acetaldehyde + 2 H(+) + triphosphate</text>
            <dbReference type="Rhea" id="RHEA:27966"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18036"/>
            <dbReference type="ChEBI" id="CHEBI:58462"/>
            <dbReference type="ChEBI" id="CHEBI:61032"/>
            <dbReference type="EC" id="4.1.2.50"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9 10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9 10">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the PTPS family. QueD subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.-.-.-" evidence="9"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315823.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2620"/>
    <dbReference type="EnsemblBacteria" id="ABA05873">
        <property type="protein sequence ID" value="ABA05873"/>
        <property type="gene ID" value="Nwi_2620"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2620"/>
    <dbReference type="eggNOG" id="COG0720">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111016_6_1_5"/>
    <dbReference type="OMA" id="KCEKLHG"/>
    <dbReference type="OrthoDB" id="1770189at2"/>
    <dbReference type="UniPathway" id="UPA00391"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070497">
        <property type="term" value="F:6-carboxy-5,6,7,8-tetrahydropterin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008616">
        <property type="term" value="P:queuosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007115">
        <property type="entry name" value="6-PTP_synth/QueD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038418">
        <property type="entry name" value="6-PTP_synth/QueD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589">
        <property type="entry name" value="PTHR12589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01242">
        <property type="entry name" value="PTPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006113">
        <property type="entry name" value="PTP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0671">Queuosine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9 10" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR006113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR006113-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA05873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="123" mass="13371" checksum="370865C22D8B8DF9" modified="2005-10-11" version="1">MWELTKSFRFEAAHALPGTTLGAASMEIHGHSFRAEVSLRGTPDPASGMVLDLGLLERGMGEVRNKLDHKLLNDVEGLGPPTLENLARFIWDHVQHAGAVTRVSVHRDSCGESCAYFGPHVPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="129" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPF2</accession>
    <name>Q3SPF2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glycine--tRNA ligase beta subunit</fullName>
            <ecNumber evidence="10">6.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Glycyl-tRNA synthetase beta subunit</fullName>
            <shortName evidence="10">GlyRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">glyS</name>
        <name evidence="12" type="ordered locus">Nwi_2586</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly)</text>
            <dbReference type="Rhea" id="RHEA:16013"/>
            <dbReference type="Rhea" id="RHEA-COMP:9664"/>
            <dbReference type="Rhea" id="RHEA-COMP:9683"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.14"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4 10">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.14" evidence="10"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315789.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2586"/>
    <dbReference type="EnsemblBacteria" id="ABA05839">
        <property type="protein sequence ID" value="ABA05839"/>
        <property type="gene ID" value="Nwi_2586"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2586"/>
    <dbReference type="eggNOG" id="COG0751">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007220_2_1_5"/>
    <dbReference type="OMA" id="LPIPKRM"/>
    <dbReference type="OrthoDB" id="213210at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006426">
        <property type="term" value="P:glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00255">
        <property type="entry name" value="Gly_tRNA_synth_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008909">
        <property type="entry name" value="DALR_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015944">
        <property type="entry name" value="Gly-tRNA-synth_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006194">
        <property type="entry name" value="Gly-tRNA-synth_heterodimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30075">
        <property type="entry name" value="PTHR30075"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05746">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02092">
        <property type="entry name" value="tRNA_synt_2f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01045">
        <property type="entry name" value="TRNASYNTHGB"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00211">
        <property type="entry name" value="glyS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50861">
        <property type="entry name" value="AA_TRNA_LIGASE_II_GLYAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DALR_1" evidence="11">
        <location>
            <begin position="617"/>
            <end position="714"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA05839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="727" mass="80214" checksum="F57B2D27FF0BD0EC" modified="2005-10-11" version="1">MPDLLLELFSEEIPARMQAKAADDLRRMVTDKLVAEGLVYEGAKAFVTPRRLALTVHGIPARQPDLKDERKGPKVGAPDAAVQGFLKAAGLTSLDEAKIQRDPKKGDFYVALIERPGRPAIDVLAELLPVIVRTFPWPKQMRWGERSAKPGALTWVRPLHSIIATFGMETEEPEVVPFDVADITAGQATRGHRFMAPGEFSVRRFEDYEAKLHDAKVVLDPQRRKDIILADAKELAFAQGYELVEDQALLDEVSGLVEWPVVLMGSFDKEFLSIPGEVIRATIRNNQKCFVVNDPKSGKLANRFLLVANLEASDGGEAIIAGNERVIRARLADAKFFYETDLKTKLEARLPKFEQIVFHEKLGTQAERIARIERLAAEIAPLVGADVEKAKRAARLCKTDLLTEVVGEFPELQGLMGKYYALAQGEDASVAAACEEHYKPQGPNDRVPSDPVSIAVALADKIDTLVGFWAIDEKPTGSKDPYALRRAALGVIRLVVNNGLQLKLRHITAEALNLLEEQKPDLVGEARQRWLNQYGGSHSIEAALESAVAAVFVKDLLIFFADRLKVQLREQGARHDLVDAVFALEGQDDLLMIVRRVEALGKFLDTDDGKNLLSGTKRASNILAIEEKKDKRSFDGAPDASLYTLGEEKALGAAIDQVKREASAAVATEDFAAAMSAMAKLRPAVDAFFEQVKVNDADPKIRENRLRLLNEIRAATRTVADFSKIQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPF4</accession>
    <name>Q3SPF4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2584</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2584"/>
    <dbReference type="EnsemblBacteria" id="ABA05837">
        <property type="protein sequence ID" value="ABA05837"/>
        <property type="gene ID" value="Nwi_2584"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2584"/>
    <dbReference type="eggNOG" id="ENOG50314SS">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_207916_1_0_5"/>
    <dbReference type="OMA" id="AIYLICV"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021446">
        <property type="entry name" value="DUF3096"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11295">
        <property type="entry name" value="DUF3096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="51" mass="5548" checksum="8763B7B8ED405188" modified="2005-10-11" version="1">MVITAAHIQPIVALIAGVLILLMPRFLNLLVAVYLIFVGLSGLGVFKMLHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPK6</accession>
    <name>Q3SPK6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2532</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315736.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2532"/>
    <dbReference type="EnsemblBacteria" id="ABA05785">
        <property type="protein sequence ID" value="ABA05785"/>
        <property type="gene ID" value="Nwi_2532"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2532"/>
    <dbReference type="eggNOG" id="ENOG5030GJ5">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117531_0_0_5"/>
    <dbReference type="OMA" id="MQHCTDE"/>
    <dbReference type="OrthoDB" id="2015776at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022061">
        <property type="entry name" value="DUF3617"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12276">
        <property type="entry name" value="DUF3617"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229094" evidence="1">
        <location>
            <begin position="25"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="176" mass="18944" checksum="2A44799B9B9D44C7" modified="2005-10-11" version="1">MRKSSRHLMFLLPLVLLSAAPALAEVQLPLRKAGLWDVKVDHEGGRMPGVAVQQCTSEAVDREFTSDFAPSARQNCSKADVQKTATGYVMDSVCTMGGTTVTSHAEATGDFDSAYTVKVAARSKGGKFGDRNSKITIAAKWVGPCKEGQTPGDVVMPGGFKMNMRDFEKFKSMAPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPR1</accession>
    <name>Q3SPR1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2477</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315683.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05730">
        <property type="protein sequence ID" value="ABA05730"/>
        <property type="gene ID" value="Nwi_2477"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2477"/>
    <dbReference type="eggNOG" id="ENOG503355X">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159800_0_0_5"/>
    <dbReference type="OMA" id="DCYWQRQ"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229243" evidence="2">
        <location>
            <begin position="20"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="105" mass="10846" checksum="967111B9CD658AF2" modified="2005-10-11" version="1">MKRTVMALATVGALVTALAAPQAAEARNGRIAAGVVGGLVGGAILGGMLAGPGYYGPGYGYYGGGYGPGYGYYGPGYVAAPGCYWQRQRFWDGFGWRLRNVRVCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPU9</accession>
    <name>Q3SPU9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2439</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05692">
        <property type="protein sequence ID" value="ABA05692"/>
        <property type="gene ID" value="Nwi_2439"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2439"/>
    <dbReference type="HOGENOM" id="CLU_1146267_0_0_5"/>
    <dbReference type="OMA" id="NNCRINF"/>
    <dbReference type="OrthoDB" id="2096458at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="233" mass="26034" checksum="61CBD3E35CB216C2" modified="2005-10-11" version="1">MGAEPASFAPENNPQRVLTRTRIRYAPPMIDPVPPPSASTAFDLYYLQNGKRFFWRNPNHGVSLIDDGHESAILWHNEGKAIRRLWTDIVSINMTAGTTGKEIVNNCRINFRNGRFLVVTDGGASGEVDHDRTPVYRDFVRALHRRLALAPPGAIRFVAGVSQTRHTVMLVTGVIAGLLFIVTPLVLTLFLRDWRALGPLAAGTVFVWPFFKVIGANRPRDYDPRHPPGELMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPV2</accession>
    <name>Q3SPV2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_2436</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315645.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2436"/>
    <dbReference type="EnsemblBacteria" id="ABA05689">
        <property type="protein sequence ID" value="ABA05689"/>
        <property type="gene ID" value="Nwi_2436"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2436"/>
    <dbReference type="eggNOG" id="COG0730">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054750_4_0_5"/>
    <dbReference type="OMA" id="FLVWGSK"/>
    <dbReference type="OrthoDB" id="1274094at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="71"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="346" mass="37606" checksum="6AB0E3A4194DC078" modified="2005-10-11" version="1">MIYLFLFAASFIAFSLSVICGGGAGLLLIPLLGYYLPVSQVPAALTLGTATSSISRIWIFFNAIRWDMARLFLPTAIVGVVLGAKLLSYLEPMYLELCMGLFLISNLPLLFRKQKSKPASSASLAIPSWQIRSVGFLAGFISGLTGAVGVLFNRFYFRCGLDNQELIATRAANEAFLHVLKLSLYASLGLFQLKTIGIGVLVALAAVLSSGFMKFALPGISRGLLMRAGYGAMVVSGVLVLNSAVGDIKVANNPDLRVTIEAKELNARLTWNTSTYFAEVSYDEGLEIETVVPLSSLSLDRQRHVRSLQGGAPKVDIEKVYALKGVSYEAYYFDGQNRLINKIKFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPX4</accession>
    <name>Q3SPX4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Aminotransferase, class V</fullName>
            <ecNumber evidence="5">2.6.1.45</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2413</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 3">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="2.6.1.45" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2413"/>
    <dbReference type="EnsemblBacteria" id="ABA05667">
        <property type="protein sequence ID" value="ABA05667"/>
        <property type="gene ID" value="Nwi_2413"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2413"/>
    <dbReference type="eggNOG" id="COG0075">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027686_1_0_5"/>
    <dbReference type="OMA" id="IRINHMG"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050281">
        <property type="term" value="F:serine-glyoxylate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024169">
        <property type="entry name" value="SP_NH2Trfase/AEP_transaminase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000524">
        <property type="entry name" value="SPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_5" evidence="4">
        <location>
            <begin position="48"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="3">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000524-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="415" mass="45487" checksum="B1AA8737D85878D9" modified="2005-10-11" version="1">MVFDLQLTHSRKGRSPMTLHTGRHTLQIPGPTNVPDRVLRAIDMPVLDHRGPEFAVLGHEVLADIKRIFRTKQPVIIYPSSGTGAWEAAIVNTLSPGDKVLTAETGQFATLWLAMAKRFNLDVDVLPTDWRRGAPLDLIDERLKADREHKIKAVMIVHNETSTSCVTHPLEVRKILDRNKHPALLLVDTISGLGAMEYEHDAWGIDVSISGSQKGMMLPAGLGFNAVSEKALAASKANTSTRYYWDWHQVIANNEQGSFPYTPATNLLYGLKVAIAMMEEEGLENVWARHKRHGAATRAAVKEWGLELVCQDPHAYSPALTAVMLPDGYDSDAFRKVVLDNFDMSLGAGLNKIKGKAFRIGHLGHQNDLMLMGALAGIEMSLDLAEVPYRAGGVAAAMEVLKGREAAPMLKAVVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ17</accession>
    <name>Q3SQ17_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ISRm2011-2 transposase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2370</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2370"/>
    <dbReference type="EnsemblBacteria" id="ABA05624">
        <property type="protein sequence ID" value="ABA05624"/>
        <property type="gene ID" value="Nwi_2370"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2370"/>
    <dbReference type="eggNOG" id="COG3335">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056788_10_0_5"/>
    <dbReference type="OMA" id="EHLWWQL"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0814">Transposable element</keyword>
    <feature type="domain" description="DDE_3" evidence="2">
        <location>
            <begin position="44"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="213" mass="24534" checksum="50FEF3674E47F04E" modified="2005-10-11" version="1">MGVRSRCEAQLQKKTLIAAEQDRPDVARRRAQWTKYRDRIDPTRLVFIDETWTKTNMAPLRGWAPRGQRIRAKVPHGRWQTMTFMAALRHDRITAPWFIEGPINGEAFLLYIEKVLVPTLRHGDIVIMDNLGSHKASAVRRVIRAAGARLFYLSKYSPDLNPIEQFFAKFKHWLRKAAQRTTEAVYNAIAPILETVAPAECANYFVNAGYNQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ86</accession>
    <name>Q3SQ86_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Siderophore synthetase component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2301</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IucA/IucC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2301"/>
    <dbReference type="EnsemblBacteria" id="ABA05555">
        <property type="protein sequence ID" value="ABA05555"/>
        <property type="gene ID" value="Nwi_2301"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2301"/>
    <dbReference type="eggNOG" id="COG4264">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018524_0_1_5"/>
    <dbReference type="OMA" id="EGHQTGH"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016881">
        <property type="term" value="F:acid-amino acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019290">
        <property type="term" value="P:siderophore biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007310">
        <property type="entry name" value="Aerobactin_biosyn_IucA/IucC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022770">
        <property type="entry name" value="FhuF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037455">
        <property type="entry name" value="LucA/IucC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34384">
        <property type="entry name" value="PTHR34384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06276">
        <property type="entry name" value="FhuF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04183">
        <property type="entry name" value="IucA_IucC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IucA_IucC" evidence="2">
        <location>
            <begin position="113"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="FhuF" evidence="3">
        <location>
            <begin position="372"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="562" mass="63561" checksum="CBBE9EEDFC829819" modified="2005-10-11" version="1">MRQLTAAALYENLFGSIDRTVRGAQGFEWSVDGRAWRSRGRLGAFGRPRLEAGTVEWRTAGEWRQASIADIVSALPGPEQARAQLGRELEQTAALMRWNCQHQPRRDRRHLAFCALDAAIDEGHPYHPCFKSRTGFDEADHRAYGPEAGNSFQLVWLAVARDLLHLALPDSEDLFWQREIGVQGWHELRRRSAALGIEGRDFGLMPIHPWQWRDLSEGRLKSLIQSGRVYYLGTAGDPYRASQSVRSLTNARDPRRSGVKLAMNIVNTSTRRTIEPHSVGTAPVLSHWLGEVIDSDPLFRGDCRLDILREHAGLIMDRDGPLGGEIAAIWRESVEDKLAEGEAAAPFNALMAVEADGRPFIDPWVSRYGVMEWLDRLIHVALLPVWRLLIAHGIATEAHGQNMVLVHRDGWPERLILRDFHDSLEFVPDFLADPALAPDFETLNPDYRNAQPNQYYWMQRPEDLRDLFVDCVFVFSLSEISHLVQSFYGIDERKFWQRVARQLSACITEHDLGARAARLGFGTREILTESLLARKLGLNGPDFQHEVPNALAGLSLLARENA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQB7</accession>
    <name>Q3SQB7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Amine oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315491.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2270"/>
    <dbReference type="EnsemblBacteria" id="ABA05524">
        <property type="protein sequence ID" value="ABA05524"/>
        <property type="gene ID" value="Nwi_2270"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2270"/>
    <dbReference type="eggNOG" id="COG1232">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022687_2_1_5"/>
    <dbReference type="OMA" id="HSMIFNQ"/>
    <dbReference type="OrthoDB" id="630753at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017830">
        <property type="entry name" value="SQase_HpnE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03467">
        <property type="entry name" value="HpnE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amino_oxidase" evidence="1">
        <location>
            <begin position="12"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Amino_oxidase" evidence="1">
        <location>
            <begin position="320"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="417" mass="44887" checksum="E27635A72865F3D0" modified="2005-10-11" version="1">MERTVHIAGGGISGLAAGVRLADAGYLVRVHEAAQQVGGRCRSYFDGATNLTIDNGNHLVLSGNNNVMAYARTIGSEAGLRGPPEAEFQFIDLAADKRWTLRINDGRVPFWVFDRTRRVPDTSPADYFALAPLIWASAGTRVGDAIKCEGVLYKRLVLPLLLAALNIDPPDGSAGLAGAIIRETLLAGGQACRPLIARDGLSSVLIEPAIGYIRARGGSVDVGHEVRRLTMAHGRVTELALGDQTIALGPEDAVVMAVPPRSAAMLLPGLTVPTRFRAIVNAHFRFVPPPHHPPMIGVVNGLIEWLFAFPDRFSITISGGDRLVDMPREELARAIWKDICAVAGLEAEMPAWQIVRERRATFEASPEQNALRPGTLTAWKNLFLAGDWTDTGLPATIEGSVRSGNRAADHVLKTRSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQC1</accession>
    <name>Q3SQC1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">4-hydroxy-3-methylbut-2-enyl diphosphate reductase</fullName>
            <shortName evidence="5">HMBPP reductase</shortName>
            <ecNumber evidence="5">1.17.7.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ispH</name>
        <name evidence="6" type="ordered locus">Nwi_2266</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>H2O + isopentenyl diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin] = (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:24488"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:128753"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="1.17.7.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>dimethylallyl diphosphate + H2O + 2 oxidized [2Fe-2S]-[ferredoxin] = (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:24825"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:128753"/>
            <dbReference type="EC" id="1.17.7.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="5">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the IspH family.</text>
    </comment>
    <dbReference type="EC" id="1.17.7.4" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2266"/>
    <dbReference type="EnsemblBacteria" id="ABA05520">
        <property type="protein sequence ID" value="ABA05520"/>
        <property type="gene ID" value="Nwi_2266"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2266"/>
    <dbReference type="eggNOG" id="COG0761">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027486_1_0_5"/>
    <dbReference type="OMA" id="NDSICRQ"/>
    <dbReference type="OrthoDB" id="1311145at2"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00097"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00059">
        <property type="reaction ID" value="UER00105"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051745">
        <property type="term" value="F:4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050992">
        <property type="term" value="P:dimethylallyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13944">
        <property type="entry name" value="lytB_ispH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00191">
        <property type="entry name" value="IspH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003451">
        <property type="entry name" value="LytB/IspH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30426">
        <property type="entry name" value="PTHR30426"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02401">
        <property type="entry name" value="LYTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00216">
        <property type="entry name" value="ispH_lytB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3 5" id="KW-0408">Iron</keyword>
    <keyword evidence="4 5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="228"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="314" mass="33649" checksum="615A71BF3F0B226C" modified="2005-10-11" version="1">MTDPRPIEVLLAQPRGFCAGVVRAIETVERALEKYGPPVYVRHEIVHNKYVVESLKNKGAIFVEDLSEVPPKAVTVFSAHGVAQSVEEEAAARGLPVLNATCPLVTKVHNQGKRYTSKGRTLILIGHAGHPEVEGTMGQVPGPVLLVQNVDDVAALTLPADTPVAYITQTTLSVDDTKDIIIALQQRFTDIQGPDTRDICYATQNRQSAVRDLSKLVDVILVVGAANSSNSNRLREIGTEVGVASYLIADGSELNAEWLEGAKAVGVTAGASAPEVLVDDVIEALKRIRPVAVSVLPGREENIEFRLPSELTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQD6</accession>
    <name>Q3SQD6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2251</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315473.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2251"/>
    <dbReference type="EnsemblBacteria" id="ABA05505">
        <property type="protein sequence ID" value="ABA05505"/>
        <property type="gene ID" value="Nwi_2251"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2251"/>
    <dbReference type="eggNOG" id="ENOG5030UCA">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084461_0_0_5"/>
    <dbReference type="OMA" id="KHHAKPA"/>
    <dbReference type="OrthoDB" id="1995749at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="217"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="240" mass="24304" checksum="01CCDA1C4C4101DB" modified="2005-10-11" version="1">MSIGASGRSALIIAAGLLLGFAGPMRATDSAAEPAGVAAATHPGQAGAPIKLGKFSKHRAHKRHAARTRKVKPEPRANVKAEETTASTAQDSNKPIPLPPTIANANASVEMPAGTAGLSGDDSPKTEASALANSAGEMLAVNQEDGALPPDNTSSPAARVDIVSSDEINEIDRTVAGNHQVAPSLALASIDIAPTSHNADTGQTAANDSSTWNQTSLIGKIFIAFGGLLMIGSAARMFMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQG4</accession>
    <name>Q3SQG4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cysteine desulfuration protein SufE</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2223</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SufE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315446.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2223"/>
    <dbReference type="EnsemblBacteria" id="ABA05477">
        <property type="protein sequence ID" value="ABA05477"/>
        <property type="gene ID" value="Nwi_2223"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2223"/>
    <dbReference type="eggNOG" id="COG2166">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124502_0_0_5"/>
    <dbReference type="OMA" id="DWMQRYE"/>
    <dbReference type="OrthoDB" id="1996347at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003808">
        <property type="entry name" value="Fe-S_metab-assoc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43597">
        <property type="entry name" value="PTHR43597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02657">
        <property type="entry name" value="SufE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SufE" evidence="2">
        <location>
            <begin position="8"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="142" mass="15970" checksum="7944F652AA92C32F" modified="2005-10-11" version="1">MTIDEIRDNFELLEEWDDRYRYVIELGRTLEPMSEAEHSAANKVQGCASQVWLSLRTSRGPDSAPVLTYVGDSDAHIVRGLIAILLTLYSGRKPQDILDTDAIAVFDELGFREHLTPQRSNGLRAMVERIRRDAREALAAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQG8</accession>
    <name>Q3SQG8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">TonB-dependent receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_2219</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Cell outer membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TonB-dependent receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315442.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2219"/>
    <dbReference type="EnsemblBacteria" id="ABA05473">
        <property type="protein sequence ID" value="ABA05473"/>
        <property type="gene ID" value="Nwi_2219"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2219"/>
    <dbReference type="eggNOG" id="COG4206">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008287_18_5_5"/>
    <dbReference type="OMA" id="TYNRIEE"/>
    <dbReference type="OrthoDB" id="71288at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039426">
        <property type="entry name" value="BtuB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012910">
        <property type="entry name" value="Plug_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037066">
        <property type="entry name" value="Plug_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000531">
        <property type="entry name" value="TonB-dep_rcpt_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036942">
        <property type="entry name" value="TonB_rcpt_b-brl_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30069">
        <property type="entry name" value="PTHR30069"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07715">
        <property type="entry name" value="Plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00593">
        <property type="entry name" value="TonB_dep_Rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0798">TonB box</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229129" evidence="8">
        <location>
            <begin position="35"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="domain" description="Plug" evidence="10">
        <location>
            <begin position="105"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="TonB_dep_Rec" evidence="9">
        <location>
            <begin position="243"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="31"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA05473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="692" mass="74791" checksum="04E145DE9477CA32" modified="2005-10-11" version="1">MSAAFVSGAYRRSLLASALLASLGSIGLNTPTSAQQSASPNLLPPIRIAPPPDRAMAPAHRTTSRSRRVVRPAPRQTPVAETHVPGTMGSTVVSPTGVVTPAGQLASSITVVTEQDIQTQQHRSVPDILRTAPGLNVVQAGGPGAQTSIFMRGTNANHTKVMLDGIDIGDPANSNGAFDYAHLLTADIQQMEILRGPQSGLYGSDAIGGVISIITKKGEGPARVTGSLESGSFKTFNQTLGLSGAERNFNYAVNVAHLHAGDVPVTPPELLPPGRQAIGNNYDNMTYSTKLGVDLNEYLTVNSVVRYTDSTLLFTGDGGFPSTPNASQSTHAVQQLFNRQEAVWSLFDGRVQSFFGLNFTNSRAYDVGPGDPAATITTGERLKFDWRTVTEITRDNHLIVGAEHQTDRMDTADFAARNGNKAGFVQLQSAFADRFFLVANVRQDSNDLFGGRMTYRIAPAVIVPVTETKLKASYGTGFKAPSLSQLFRDYPTFNFFANPNLQPEDSRGFDAGFEQPLFNDRVRFGSTYFRNDITNLIDYNSTFTSLVNVNSATTEGTETFVAAQITERFGIRADYTFIRAVNAATGMQLLRRPKEKWSATATWLPLDALTLSATLVRVNDWLDVTRDGMASGITVPGYTLVNLRGDYALSDQVKVFGRIDNLLDFRYQNPTGFLAPGLAVFGGIRVASYGVR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-12-20" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQJ9</accession>
    <name>XGPT_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Xanthine-guanine phosphoribosyltransferase</fullName>
            <shortName evidence="1">XGPRT</shortName>
            <ecNumber evidence="1">2.4.2.-</ecNumber>
            <ecNumber evidence="1">2.4.2.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Xanthine phosphoribosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gpt</name>
        <name type="ordered locus">Nwi_2188</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Purine salvage pathway enzyme that catalyzes the transfer of the ribosyl-5-phosphate group from 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to the N9 position of the 6-oxopurines guanine and xanthine to form the corresponding ribonucleotides GMP (guanosine 5'-monophosphate) and XMP (xanthosine 5'-monophosphate), with the release of PPi. To a lesser extent, also acts on hypoxanthine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>diphosphate + GMP = 5-phospho-alpha-D-ribose 1-diphosphate + guanine</text>
            <dbReference type="Rhea" id="RHEA:25424"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="1">
            <dbReference type="Rhea" id="RHEA:25426"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>diphosphate + XMP = 5-phospho-alpha-D-ribose 1-diphosphate + xanthine</text>
            <dbReference type="Rhea" id="RHEA:10800"/>
            <dbReference type="ChEBI" id="CHEBI:17712"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.22"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="1">
            <dbReference type="Rhea" id="RHEA:10802"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>diphosphate + IMP = 5-phospho-alpha-D-ribose 1-diphosphate + hypoxanthine</text>
            <dbReference type="Rhea" id="RHEA:17973"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="1">
            <dbReference type="Rhea" id="RHEA:17975"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; GMP biosynthesis via salvage pathway; GMP from guanine: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; XMP biosynthesis via salvage pathway; XMP from xanthine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the purine/pyrimidine phosphoribosyltransferase family. XGPT subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.-" evidence="1"/>
    <dbReference type="EC" id="2.4.2.22" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315411.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SQJ9"/>
    <dbReference type="SMR" id="Q3SQJ9"/>
    <dbReference type="STRING" id="323098.Nwi_2188"/>
    <dbReference type="EnsemblBacteria" id="ABA05442">
        <property type="protein sequence ID" value="ABA05442"/>
        <property type="gene ID" value="Nwi_2188"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2188"/>
    <dbReference type="eggNOG" id="COG2236">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080904_3_0_5"/>
    <dbReference type="OMA" id="FHRDCRA"/>
    <dbReference type="OrthoDB" id="1819460at2"/>
    <dbReference type="UniPathway" id="UPA00602">
        <property type="reaction ID" value="UER00658"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00909">
        <property type="reaction ID" value="UER00887"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052657">
        <property type="term" value="F:guanine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004422">
        <property type="term" value="F:hypoxanthine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000310">
        <property type="term" value="F:xanthine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032263">
        <property type="term" value="P:GMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032265">
        <property type="term" value="P:XMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01903">
        <property type="entry name" value="XGPRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023747">
        <property type="entry name" value="Xanthine_Guanine_PRibTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39563">
        <property type="entry name" value="PTHR39563"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00103">
        <property type="entry name" value="PUR_PYR_PR_TRANSFER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0660">Purine salvage</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000139676" description="Xanthine-guanine phosphoribosyltransferase">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="43"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="102"/>
            <end position="110"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="106"/>
            <end position="110"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>guanine</name>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>xanthine</name>
            <dbReference type="ChEBI" id="CHEBI:17712"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="148"/>
            <end position="149"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>guanine</name>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>xanthine</name>
            <dbReference type="ChEBI" id="CHEBI:17712"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01903"/>
        </source>
    </evidence>
    <sequence length="168" mass="18321" checksum="43E92B651FA20CAD" modified="2005-10-11" version="1">MSAHKTPDKAFPVSWDQFHRDCRALSWRLNETGPFHAVVAITRGGLVPAAIVARELGVRVIDTVCIASYDHLHKSELKVLKGISDMTVKLGGGSGRNLLVVDDLVDTGATARVVRGMIPDAHFATVYAKPLGKPLVDTFITEVSQDTWIYFPWDLDLAFAPPLRDGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQL8</accession>
    <name>Q3SQL8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphate-selective porin O and P</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2169</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315392.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2169"/>
    <dbReference type="EnsemblBacteria" id="ABA05423">
        <property type="protein sequence ID" value="ABA05423"/>
        <property type="gene ID" value="Nwi_2169"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2169"/>
    <dbReference type="eggNOG" id="COG3746">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040285_0_0_5"/>
    <dbReference type="OMA" id="WNWGRGP"/>
    <dbReference type="OrthoDB" id="1578713at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010870">
        <property type="entry name" value="Porin_O/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07396">
        <property type="entry name" value="Porin_O_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229145" evidence="2">
        <location>
            <begin position="28"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="475" mass="53776" checksum="AB784C963C869878" modified="2005-10-11" version="1">MNKKKFNAAVCGFGLVLATSQLSPAGAQEMELYVDTVTKQVYTEPGKNRVMMGTFQQIKKGSGQRAQRGSSRQAQQGSGQTVAHRTVTQDNDLPVVKSREKKEWYDKLKIRGYTQFRSASTVGGDKDAVSYWPDKSVGENSSFLIRRARVILYGDVSDNLYVYLQPDFASTPSGSSTGHFGQLRDAYADIAFDQKKEFRVRVGQSKIPFSFENLQSSQNRLALDRNDGLNSCCRDERDIGAFFYWAPAHIRERFKDLVSNNLKGSGDYGVFAFGVYNGQGANRVEQNNDMHMVVRFTYPYMFGNGQIFEAGVQAVHGRFVTSTGPINGVTPIMDAPAKGFKDQRVGVHAVLYPQPFGFQAEWNWGRGPQLNDTQTMLTESSLSGGYVLANYRLNAGQWGMLFPFVKWQHFEGGQKFERNAPHNYVNDWEFGVEWQVTKEIELTAVYHMMNRTDLANAPYERYKADVLRFQLQWNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQM9</accession>
    <name>Q3SQM9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Acyl-CoA dehydrogenase</fullName>
            <ecNumber evidence="9">1.3.8.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_2158</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.1" evidence="9"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315381.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2158"/>
    <dbReference type="EnsemblBacteria" id="ABA05412">
        <property type="protein sequence ID" value="ABA05412"/>
        <property type="gene ID" value="Nwi_2158"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2158"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_2_5"/>
    <dbReference type="OMA" id="NMATWML"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004085">
        <property type="term" value="F:butyryl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0274">FAD</keyword>
    <keyword evidence="3 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="8">
        <location>
            <begin position="6"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="7">
        <location>
            <begin position="121"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="6">
        <location>
            <begin position="227"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="381" mass="41323" checksum="C6BD6C4F309C72E6" modified="2005-10-11" version="1">MQFALTEEQIAVRDMARDVANRKIAPFALQWDEEKHFPVETMREAAALGIGGICIRNDVGGSALSRLDGTLIFEALAQGCPTVSSFISIHNMASWMIDAHGSDEQRRKWLPKLCTMDLLASYCLTEPGAGSDAAALRTRARRDGDHYVVDGQKQFISGAGASDLYVVMVRTGDHGPDGVSALVVERGTPGVSFGANERKMGWNAQPTRAVIFENARVPVANRIGAEGAGFRIAMAGLDGGRLNIAACSLGGAQSALDKALGYMKERKAFGKRLDEFQALQFKVADMATELEAARTFLWRAASALDREDTNATMLCAVAKRLATDVGFDVANQALQLHGGYGYLSEYGIEKIVRDLRVHQILEGTNEIMRLIVSRKVIEGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQP6</accession>
    <name>Q3SQP6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Prophage CP4-57 regulatory</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2141"/>
    <dbReference type="EnsemblBacteria" id="ABA05395">
        <property type="protein sequence ID" value="ABA05395"/>
        <property type="gene ID" value="Nwi_2141"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2141"/>
    <dbReference type="eggNOG" id="COG3311">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140176_2_0_5"/>
    <dbReference type="OMA" id="SRCVVWD"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010260">
        <property type="entry name" value="AlpA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05930">
        <property type="entry name" value="Phage_AlpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="98" mass="11444" checksum="5ABFADB3A7549113" modified="2005-10-11" version="1">MTNRNGAPIKRTIRRHQLREMVPLADSTIYEMEQRGEFPRRFALSPRCVVWDLAEVEAWLAARRASPVRRAQHPDVRQRRARPVRERDQARGEASKAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQR8</accession>
    <name>Q3SQR8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Heat shock protein Hsp20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2118</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315346.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2118"/>
    <dbReference type="EnsemblBacteria" id="ABA05373">
        <property type="protein sequence ID" value="ABA05373"/>
        <property type="gene ID" value="Nwi_2118"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2118"/>
    <dbReference type="eggNOG" id="COG0071">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046737_12_0_5"/>
    <dbReference type="OMA" id="QMERMFD"/>
    <dbReference type="OrthoDB" id="1523369at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="SSF49764"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="55"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="166" mass="19267" checksum="2E4E918C3C149A44" modified="2005-10-11" version="1">MSMRDLIPWNRSSRVPVQRGEEGTPFLTLHREMNRLFDDVFRGLDLNPFRFDRVFDRAWSWPSIEVSENDKEVRVTAELPGLEEKDVEVQLSNGVLAIKGEKRTETEDNDRLFSERFYGRFERRIPVDDIDEDKVNASFGNGVLVVTLPRSAEAQRNVKRIAINGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQU6</accession>
    <name>Q3SQU6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Y4BN protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2088</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315323.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2088"/>
    <dbReference type="EnsemblBacteria" id="ABA05345">
        <property type="protein sequence ID" value="ABA05345"/>
        <property type="gene ID" value="Nwi_2088"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2088"/>
    <dbReference type="eggNOG" id="COG1404">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017730_1_0_5"/>
    <dbReference type="OMA" id="HTDINQF"/>
    <dbReference type="OrthoDB" id="325722at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04847">
        <property type="entry name" value="Peptidases_S8_Subtilisin_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034074">
        <property type="entry name" value="Y4bN_pept_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="SSF52743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_S8" evidence="1">
        <location>
            <begin position="288"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="847" mass="91593" checksum="BB7E963313A231B8" modified="2005-10-11" version="1">MAEPDDYGARNRAHISINAFRETAAYTFPVRNQERKPLREDYAAHAATILDQLAVALGNLPPPNADTRLAVPGLRRGTIVEVTTVPPAEGSRTKAIKVPSTLEFPTQDVVVLRSERNDDRTESALLFVPDDAREFLRTRITDYGRDPGNQRRPHVDRFEAVETVRMTEARSLFSADVDVAMPDVVWWELWVRQPIVIADRLAELARGANLDVHADRLVFPDTTVLFVHAAAAALVAFAARVPGALTEIRRATGTIEPFLDRGATGLGQHDWVTELAGRVTPPAGDIPAICALDTGVNAAHPLIAPGLAGAWAYDAAWNADDHHPDGGHGTPLAGLALYGDLEPLMNDAQPVALTHAVESMKLLPPNGFPPTKPPSYGVVTQGAVVSVEIARPNVRRSFCIAISATDFPPDRPSTWSGALDQVAAGSMPGDSADGVPASQRPKRLMLTATGNVLGGMMVDVVPSQPLEDPAQSWNTLTIGGFTRKEQPPAPPPALEPVVPANHRSPFSRGSQTLPDDLTPIKPEVLFEAGNMLSDASGSCDWGPSVSLLAPGSDVVAEPLVPFWATSAAVGMAGNFIGRLQAALPTRWPETHRALTVDSAQWPQPIRRKLIGRGASWKTGSKAEKQQILREVGFGVPDIERAILSASNDVTLIAEAEIQPFAFSADGRSGVFNEMHFYDLPWPRTVLERIENESVMMKVTLSYFIEPNLTGKAATRPDTYRSFGLRFAMKNRTETDARFRSRISASQAKDGTEPEGEKSYWLLGPKAIQAGSLHCDLWRGPAIELASHDAIAVFPVGGWWKSHVGQRRVADKARYSLVISISAPGQAVDLYSEIAALVEVKELEVSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQU9</accession>
    <name>Q3SQU9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2085</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2085"/>
    <dbReference type="EnsemblBacteria" id="ABA05342">
        <property type="protein sequence ID" value="ABA05342"/>
        <property type="gene ID" value="Nwi_2085"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2085"/>
    <dbReference type="HOGENOM" id="CLU_933292_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="298" mass="33790" checksum="4D171E2E03123833" modified="2005-10-11" version="1">MLRQSLVRQSKPGEQAIGLLRAALDDTAQDFTAQTRLLAENVDPKYRDDILRAGTNYSSLGYALVGGDGTLIEIPNARALNERVAIEMHRYANYGWGSFLPLNVPERAPQVRTSTLAGEEVTYLEGMRVENTSLISSAFDYWRIYERGICVSVESYRDDWRREGDAAPPHLTPMWILITIHSLLAHARLAGQELLGVTQVVIRMDWHGLKGRMLAWDHFRHVAGGGTLADDHFAKNIVFDWALLRDNYFETLRRVALPFLQVFGNAGWFNPDDWLTREAVEREFSRTGANTVKLLEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR00</accession>
    <name>Q3SR00_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2032</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0111 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2032"/>
    <dbReference type="EnsemblBacteria" id="ABA05291">
        <property type="protein sequence ID" value="ABA05291"/>
        <property type="gene ID" value="Nwi_2032"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2032"/>
    <dbReference type="eggNOG" id="COG1392">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086031_2_1_5"/>
    <dbReference type="OMA" id="LFNGKYD"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018445">
        <property type="entry name" value="Put_Phosphate_transp_reg"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01865">
        <property type="entry name" value="PhoU_div"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="215" mass="24448" checksum="3B8B176AABA85814" modified="2005-10-11" version="1">MLLRLFRFFLPKEERFFHLFSRHSQTVMLGAEALQGMLKGGEETPVFCQRVSQLENDADGITREVLTAVRRTFITPFDRVDIKNLITSMDDAIDQMQQTAKAVVLFEVRSFEPPMREIGSLIVECANLVHRALPLLESISNNVAMLMAITEELGKLEGRVDDLHDIGLKELFLKHRNANAMDFIVGAEIYDHLEKVADRFDDVANEISSIVIEQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR16</accession>
    <name>Q3SR16_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Colicin V production protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2016</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315261.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2016"/>
    <dbReference type="EnsemblBacteria" id="ABA05275">
        <property type="protein sequence ID" value="ABA05275"/>
        <property type="gene ID" value="Nwi_2016"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2016"/>
    <dbReference type="eggNOG" id="COG1286">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092720_0_1_5"/>
    <dbReference type="OMA" id="FTWVDWA"/>
    <dbReference type="OrthoDB" id="2032521at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009403">
        <property type="term" value="P:toxin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003825">
        <property type="entry name" value="Colicin-V_CvpA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02674">
        <property type="entry name" value="Colicin_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="167"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="210" mass="22870" checksum="C1CF738958F9DEEC" modified="2005-10-11" version="1">MPITILDLVLLGVMLISGLLAMIRGFMREILSIASWGIAALATLYSFQKLLPFAKNYFNNDTIAAAAVIGGVFIGTLIVISILTTRISDMVLDSRIGALDRTLGFLFGLARGLLIVVVAFLFFTWLVPDKQRPDWVTGAKSLTVLNSTGTWLQSLLPDDPENTILNKLKKNKPEVEQPDSEPAESPGSDGYSKPARDSLQKLIDGKPAEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR52</accession>
    <name>Q3SR52_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Carboxysome structural peptide CsoS1B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1980</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315226.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1980"/>
    <dbReference type="EnsemblBacteria" id="ABA05239">
        <property type="protein sequence ID" value="ABA05239"/>
        <property type="gene ID" value="Nwi_1980"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1980"/>
    <dbReference type="eggNOG" id="COG4577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064903_5_3_5"/>
    <dbReference type="OMA" id="QFREGVN"/>
    <dbReference type="OrthoDB" id="1802372at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031469">
        <property type="term" value="C:bacterial microcompartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020808">
        <property type="entry name" value="Bact_microcomp_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000249">
        <property type="entry name" value="BMC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037233">
        <property type="entry name" value="CcmK-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044872">
        <property type="entry name" value="CcmK/CsoS1_BMC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00936">
        <property type="entry name" value="BMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00877">
        <property type="entry name" value="BMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143414">
        <property type="entry name" value="SSF143414"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01139">
        <property type="entry name" value="BMC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51930">
        <property type="entry name" value="BMC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1283">Bacterial microcompartment</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BMC" evidence="3">
        <location>
            <begin position="9"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="113" mass="11495" checksum="C60A563B7978FC03" modified="2005-10-11" version="1">MATHVNGIALGMIETRGLVPAIEAADAMTKASEVRLIGRQFVGGGYVTVLVRGETGAVNAAVRAGADACERVGDGLVAAHIIARVHSEVEGILPTKPDSNGGGRDAETTSTRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR76</accession>
    <name>Q3SR76_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1955</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315206.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1955"/>
    <dbReference type="EnsemblBacteria" id="ABA05215">
        <property type="protein sequence ID" value="ABA05215"/>
        <property type="gene ID" value="Nwi_1955"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1955"/>
    <dbReference type="eggNOG" id="COG1630">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_716910_0_0_5"/>
    <dbReference type="OrthoDB" id="577691at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="380" mass="43069" checksum="FD45CC2EC38EEE40" modified="2005-10-11" version="1">MLEPASQTALRDEIARQIKEDQAVLTQLRAEVRPLVSQTRRISPRSATSISLVAADGGNNQLRFDPFLVQLVRVVDSSNNEYCLEAVSPTMSVSALSARQFSDPERPTALGRMMQYLGVEELSKLSHMIRPDDSGRPNSPSWVQVYRELVEWAILFAIVREKDFATDTLIVFDGFLRSKVFAKDLFQRYREGIQEGIDRHLKSNRRRVYLVGVAKHSKVLSRYRLSMALEGVMRSPFPCYVEIPRELEEKAYLWSEYARGDDSVTEGGEINKFVGGKMFFVKFGSGPRDPVWPIDIFLSQSTDHATIFGYLLNDAVEGFPVPLYPRSLQKAHENAALVDFDMDILQDMIFSGLRTTLGTEAPALDVFRLQDADPASRRYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRC3</accession>
    <name>Q3SRC3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1908</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315164.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1908"/>
    <dbReference type="EnsemblBacteria" id="ABA05168">
        <property type="protein sequence ID" value="ABA05168"/>
        <property type="gene ID" value="Nwi_1908"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1908"/>
    <dbReference type="eggNOG" id="ENOG5032TCM">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119063_1_0_5"/>
    <dbReference type="OMA" id="PWGAVVW"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046161">
        <property type="entry name" value="DUF6163"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19660">
        <property type="entry name" value="DUF6163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="158" mass="17243" checksum="D5F5DA30C9B9BAFA" modified="2005-10-11" version="1">MSILMSKTSSREQVSRDQAMLVAGSPTARTETDDNLWTGRLVVFLRVMAMLSLAVGLYHWAQVTGFIGGEDDAFENQTVAWQAATVYFAVIELVAAVGLWLATPWGAVVWLTTVVSMAVVELMFPVIYGGNLVIVALETAMLMAYLGLAWMAARERPP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-12-16" modified="2022-05-25" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRE6</accession>
    <name>NUOD_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADH-quinone oxidoreductase subunit D</fullName>
            <ecNumber evidence="1">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">NADH dehydrogenase I subunit D</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">NDH-1 subunit D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nuoD</name>
        <name type="ordered locus">Nwi_1885</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">NDH-1 is composed of 14 different subunits. Subunits NuoB, C, D, E, F, and G constitute the peripheral sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 49 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315141.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRE6"/>
    <dbReference type="SMR" id="Q3SRE6"/>
    <dbReference type="STRING" id="323098.Nwi_1885"/>
    <dbReference type="EnsemblBacteria" id="ABA05145">
        <property type="protein sequence ID" value="ABA05145"/>
        <property type="gene ID" value="Nwi_1885"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1885"/>
    <dbReference type="eggNOG" id="COG0649">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015134_1_1_5"/>
    <dbReference type="OMA" id="IMGTSME"/>
    <dbReference type="OrthoDB" id="473681at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050136">
        <property type="term" value="F:NADH dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01358">
        <property type="entry name" value="NDH1_NuoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001135">
        <property type="entry name" value="NADH_Q_OxRdtase_suD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014029">
        <property type="entry name" value="NADH_UbQ_OxRdtase_49kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022885">
        <property type="entry name" value="NDH1_su_D/H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11993">
        <property type="entry name" value="PTHR11993"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00346">
        <property type="entry name" value="Complex1_49kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="SSF56762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01962">
        <property type="entry name" value="NuoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00535">
        <property type="entry name" value="COMPLEX1_49K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0874">Quinone</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <keyword id="KW-0830">Ubiquinone</keyword>
    <feature type="chain" id="PRO_0000357874" description="NADH-quinone oxidoreductase subunit D">
        <location>
            <begin position="1"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01358"/>
        </source>
    </evidence>
    <sequence length="402" mass="45401" checksum="D78ECA7C15E9928C" modified="2005-10-11" version="1">MTEALGTVTPGMRNFTINFGPQHPAAHGVLRLVLELDGEVVERVDPHIGLLHRGTEKLIEQKTYLQAIPYFDRLDYVAPMNQEHAFCLAAERLLGIAVPRRGQLIRVLYCEIGRILSHLLNVTTQAMDVGALTPPLWGFEEREKLMMFYERASGSRMHAAYFRIGGVHQDLPPRLIDDIEAWCDPFLQVVADLDRLLTANRIFKQRNVDIGVVTLEQAWEWGFSGVMVRGSGAAWDLRKAQPYECYAEMDFDIPIGKNGDCYDRYLIRMEEMRQSVRIMKQCIAKLRAPDGQGPVAVEDNKIFPPRRGEMKRSMEALIHHFKLYTEGFHVPAGEIYAAVEAPKGEFGVYLVSDGSNKPYKCKIRAPGFAHLQAMDFICRGHLLADVSAILGSLDIVFGEVDR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRI1</accession>
    <name>LPXD1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UDP-3-O-acylglucosamine N-acyltransferase 1</fullName>
            <ecNumber evidence="1">2.3.1.191</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lpxD1</name>
        <name type="ordered locus">Nwi_1850</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a (3R)-hydroxyacyl-[ACP] + a UDP-3-O-[(3R)-3-hydroxyacyl]-alpha-D-glucosamine = a UDP-2-N,3-O-bis[(3R)-3-hydroxyacyl]-alpha-D-glucosamine + H(+) + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:53836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="ChEBI" id="CHEBI:137740"/>
            <dbReference type="ChEBI" id="CHEBI:137748"/>
            <dbReference type="EC" id="2.3.1.191"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Bacterial outer membrane biogenesis; LPS lipid A biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the transferase hexapeptide repeat family. LpxD subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.191" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315106.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRI1"/>
    <dbReference type="SMR" id="Q3SRI1"/>
    <dbReference type="STRING" id="323098.Nwi_1850"/>
    <dbReference type="EnsemblBacteria" id="ABA05110">
        <property type="protein sequence ID" value="ABA05110"/>
        <property type="gene ID" value="Nwi_1850"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1850"/>
    <dbReference type="eggNOG" id="COG1044">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049865_0_2_5"/>
    <dbReference type="OMA" id="QIQIAHN"/>
    <dbReference type="OrthoDB" id="1602061at2"/>
    <dbReference type="UniPathway" id="UPA00973"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016410">
        <property type="term" value="F:N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009245">
        <property type="term" value="P:lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03352">
        <property type="entry name" value="LbH_LpxD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00523">
        <property type="entry name" value="LpxD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007691">
        <property type="entry name" value="LpxD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020573">
        <property type="entry name" value="UDP_GlcNAc_AcTrfase_non-rep"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43378">
        <property type="entry name" value="PTHR43378"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04613">
        <property type="entry name" value="LpxD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01853">
        <property type="entry name" value="lipid_A_lpxD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0441">Lipid A biosynthesis</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000264400" description="UDP-3-O-acylglucosamine N-acyltransferase 1">
        <location>
            <begin position="1"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00523"/>
        </source>
    </evidence>
    <sequence length="362" mass="37844" checksum="BAA900BB93AE1A48" modified="2005-10-11" version="1">MTQPAFFKRPLPSTLAEIAASTGAQLVDASRGGIRIIGLASLDQAGPMHLAFFDNHKYAGQLAATKAGACLVSPRFEADVPAHVAVLRSKAPFRAFVSIARDFHGDALRPQSWFDNTAVAASAVIHPSAYLEDEVVIDPLAVIGPDVQIGRGSVIGSGAVIGPGVRIGRDCNVGAGTTIQATLIGNNVLIHPGCHIGQDGYGFIFFGSEGHVKVPQTGRVLIQNDVEIGAGTTIDRGSLRDTVIGEGTKIDNQVQIGHNVTIGRRCLLAAQIGLAGSLTIGDNVALGAKVGINNHLHIGDGAQVTAMSGVKDDIPANGRWGGYFAKPTRQWFRELLAVERLVRDGAPDAGSPKAAPKDRVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="127" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRJ2</accession>
    <name>Q3SRJ2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Cyclic pyranopterin monophosphate synthase</fullName>
            <ecNumber evidence="3 5">4.6.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Molybdenum cofactor biosynthesis protein C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">moaC</name>
        <name evidence="7" type="ordered locus">Nwi_1839</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the conversion of (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate to cyclic pyranopterin monophosphate (cPMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate = cyclic pyranopterin phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:49580"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59648"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.6.1.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 5">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homohexamer; trimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the MoaC family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.17" evidence="3 5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315095.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1839"/>
    <dbReference type="EnsemblBacteria" id="ABA05099">
        <property type="protein sequence ID" value="ABA05099"/>
        <property type="gene ID" value="Nwi_1839"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1839"/>
    <dbReference type="eggNOG" id="COG0315">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074693_1_1_5"/>
    <dbReference type="OMA" id="IWDMVKS"/>
    <dbReference type="OrthoDB" id="1595361at2"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061799">
        <property type="term" value="F:cyclic pyranopterin monophosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01420">
        <property type="entry name" value="MoaC_PE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01224_B">
        <property type="entry name" value="MoaC_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023045">
        <property type="entry name" value="Mo_CF_biosynth-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036522">
        <property type="entry name" value="MoaC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002820">
        <property type="entry name" value="Mopterin_CF_biosynth-C_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01967">
        <property type="entry name" value="MoaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55040">
        <property type="entry name" value="SSF55040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00581">
        <property type="entry name" value="moaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 5" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoaC" evidence="6">
        <location>
            <begin position="21"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="81"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="119"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="169" mass="17839" checksum="CE04B39F3486EBE1" modified="2005-10-11" version="1">MADKKNKPALTHIDVKGEARMVDVSAKPATERVAVAEGQVVMTKATLALIESGNAKKGDVLGTARIAGIMAAKRTSELIPLCHPLALSKVTLDIETDKKIPGCRVRASVKVSGPTGVEMEALTAVSVACLTIYDMIKAVERGVRIEGIHLVEKKGGKSGHYLVTDNAPE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRQ1</accession>
    <name>TATA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sec-independent protein translocase protein TatA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tatA</name>
        <name type="ordered locus">Nwi_1779</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes. TatA could form the protein-conducting channel of the Tat system.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The Tat system comprises two distinct complexes: a TatABC complex, containing multiple copies of TatA, TatB and TatC subunits, and a separate TatA complex, containing only TatA subunits. Substrates initially bind to the TatABC complex, which probably triggers association of the separate TatA complex to form the active translocon.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TatA/E family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315036.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRQ1"/>
    <dbReference type="SMR" id="Q3SRQ1"/>
    <dbReference type="STRING" id="323098.Nwi_1779"/>
    <dbReference type="EnsemblBacteria" id="ABA05040">
        <property type="protein sequence ID" value="ABA05040"/>
        <property type="gene ID" value="Nwi_1779"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1779"/>
    <dbReference type="eggNOG" id="COG1826">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086034_5_0_5"/>
    <dbReference type="OMA" id="KDGMREG"/>
    <dbReference type="OrthoDB" id="1907955at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033281">
        <property type="term" value="C:TAT protein transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043953">
        <property type="term" value="P:protein transport by the Tat complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00236">
        <property type="entry name" value="TatA_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003369">
        <property type="entry name" value="TatA/B/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006312">
        <property type="entry name" value="TatA/E"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02416">
        <property type="entry name" value="TatA_B_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01411">
        <property type="entry name" value="tatAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0653">Protein transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0811">Translocation</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_1000044413" description="Sec-independent protein translocase protein TatA">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="43"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="43"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="78" mass="8336" checksum="C4F56EFEBB2E75E8" modified="2005-10-11" version="1">MGSLSIWHWIVVVAVILLLFGRGKISDLMGDVAQGIKAFKKGMKDDEKTAEKPEPVKTINHNADGSGAARSDTGSKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRQ3</accession>
    <name>Q3SRQ3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sec-independent protein translocase protein TatC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">tatC</name>
        <name evidence="6" type="ordered locus">Nwi_1777</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes. Together with TatB, TatC is part of a receptor directly interacting with Tat signal peptides.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">The Tat system comprises two distinct complexes: a TatABC complex, containing multiple copies of TatA, TatB and TatC subunits, and a separate TatA complex, containing only TatA subunits. Substrates initially bind to the TatABC complex, which probably triggers association of the separate TatA complex to form the active translocon.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell inner membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TatC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315034.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1777"/>
    <dbReference type="EnsemblBacteria" id="ABA05038">
        <property type="protein sequence ID" value="ABA05038"/>
        <property type="gene ID" value="Nwi_1777"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1777"/>
    <dbReference type="eggNOG" id="COG0805">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031942_1_0_5"/>
    <dbReference type="OMA" id="EMSFLDH"/>
    <dbReference type="OrthoDB" id="1848013at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033281">
        <property type="term" value="C:TAT protein transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043953">
        <property type="term" value="P:protein transport by the Tat complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00902">
        <property type="entry name" value="TatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002033">
        <property type="entry name" value="TatC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30371">
        <property type="entry name" value="PTHR30371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00902">
        <property type="entry name" value="TatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01840">
        <property type="entry name" value="TATCFAMILY"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00945">
        <property type="entry name" value="tatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="168"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="206"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="269" mass="29457" checksum="DD48C1CB9C993E59" modified="2005-10-11" version="1">MSDEDIEASKAPLMDHLIELRSRLIKALIGFGVAFVACFFFARQIYNILIWPFVWVAGPENSKFIYTALLEYFITQLKLALFGAAFISFPIVATQIYKFVAPGLYKHERNAFLPYLIATPAFFVLGATVVYFAVLPMLVRFSLGMQQMAGPETAEIQLLPKVGEFLSLTMSLIFAFGIAFQLPVILTLLGRIGVVTSKGLREKRRYFIVGAFVIAAVLTPPDVLSQMSLALPLLLLYEGSILAVAIVEKKAARPSASAATSPPAGNPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRU4</accession>
    <name>Q3SRU4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Propionyl-CoA carboxylase</fullName>
            <ecNumber evidence="4">6.4.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">Nwi_1736</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + hydrogencarbonate + propanoyl-CoA = (S)-methylmalonyl-CoA + ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23720"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57327"/>
            <dbReference type="ChEBI" id="CHEBI:57392"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.4.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:23721"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Metabolic intermediate metabolism; propanoyl-CoA degradation; succinyl-CoA from propanoyl-CoA: step 1/3.</text>
    </comment>
    <dbReference type="EC" id="6.4.1.3" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314993.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1736"/>
    <dbReference type="EnsemblBacteria" id="ABA04997">
        <property type="protein sequence ID" value="ABA04997"/>
        <property type="gene ID" value="Nwi_1736"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1736"/>
    <dbReference type="eggNOG" id="COG4770">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000395_3_1_5"/>
    <dbReference type="OMA" id="IGPKHYS"/>
    <dbReference type="OrthoDB" id="361205at2"/>
    <dbReference type="UniPathway" id="UPA00945">
        <property type="reaction ID" value="UER00908"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004658">
        <property type="term" value="F:propionyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CbamoylP_synth_lsu-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041265">
        <property type="entry name" value="PCC_BT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18140">
        <property type="entry name" value="PCC_BT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="SSF51246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0092">Biotin</keyword>
    <keyword evidence="5 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="14">
        <location>
            <begin position="1"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="13">
        <location>
            <begin position="120"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="12">
        <location>
            <begin position="595"/>
            <end position="671"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABA04997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="671" mass="72987" checksum="DEA5741F6A25A826" modified="2005-10-11" version="1">MFKRILIANRGEIACRIIRSARRMGVETVAVYSEADRDALHVKMADEALLIGSPPASESYLVIEKIVEACRKSGAEAVHPGYGFLSEREGFPRALADAGIVFIGPHPQAIAAMGDKIESKMVAASAGVSTVPGFLGVIEDDRHAVKIAGEIGYPVMIKASAGGGGKGMRIAHSSAEVAEGFNLAKAEAKASFGDDRVFIEKFIVEPRHIEIQVLGDKHGNVIHLGERECSIQRRNQKVIEEAPSPLLDEITRRRMGEQAVALAKAVNYDSAGTVEFVVGQDKSFYFLEMNTRLQVEHPVTELVTGIDLVEQMIRIAAGEKLSVAQKDVTLTGWAVETRVYAEDPFRNFLPSVGRLVKYRPPAEMSRDDVTVRNDTGVYEGSEISLHYDPMIAKLVTHGPSRVAAIEAQAQALDAFYIDGIRHNIPFLSALMHHARWRAGNLSTGFIAEEFPNGFTARAPEGEIARRLAAVAAAIDHVLGERKRRISGQMNGRPVTREKRRAVWLDRDEILLDVWRDGDAIAVRFVDADESAGEAHRLLSRWAPGQPVWEGTFDAHPIAMQVRPITGGFRLAYQGFEAAVSVFTQTEAAAARLMPVATVTDNGKKLLCPMPGLVVSIAVTEGQEVKAGETLAVIEAMKMQNVLRAEQDGTVKRIAAVPGATLAVDALILEFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRX2</accession>
    <name>Q3SRX2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF218 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1708</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1708"/>
    <dbReference type="EnsemblBacteria" id="ABA04969">
        <property type="protein sequence ID" value="ABA04969"/>
        <property type="gene ID" value="Nwi_1708"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1708"/>
    <dbReference type="eggNOG" id="COG1434">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053514_1_2_5"/>
    <dbReference type="OMA" id="LWFTRWQ"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06259">
        <property type="entry name" value="YdcF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003848">
        <property type="entry name" value="DUF218"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02698">
        <property type="entry name" value="DUF218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="DUF218" evidence="2">
        <location>
            <begin position="95"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="280" mass="30937" checksum="46DC6DC42F5A728F" modified="2005-10-11" version="1">MSRDFRLRIDRAADRMFFLLSKVVGFIVLPSNAIALICGLGLCLSLSRWRRAGVGLLMAGIAALLIFGYSPLGNVLLLTLSERFPAWQHDGRDPDGIIVLGGAVDADISVSRGAVETNASAERVIAALELARRFPKARIVYSGGAGNLIAGLRAEAPVMGRLFEQFGIPRERIVLETRSRTTDENARFTRELVKPESGERWLLVTSAYHMPRSVGVFRKAGFDVEPYPVDWRTRGWIDAWMPFDKLSAGLARADTAAHEWVGLLVYRMTGRSDAFFPGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRY7</accession>
    <name>Q3SRY7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1693</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1693"/>
    <dbReference type="EnsemblBacteria" id="ABA04954">
        <property type="protein sequence ID" value="ABA04954"/>
        <property type="gene ID" value="Nwi_1693"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1693"/>
    <dbReference type="eggNOG" id="ENOG502ZB2B">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073303_1_0_5"/>
    <dbReference type="OMA" id="YYFAVRI"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018710">
        <property type="entry name" value="DUF2232"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09991">
        <property type="entry name" value="DUF2232"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="259"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="293"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="336" mass="34809" checksum="25991AC7A011DDF7" modified="2005-10-11" version="1">MTMNLPVAIAAGCASALMFASIISGALISVLLFYLAPLPLMVAAMGWGPACAAIGGAFAAVSLGALFGASYSAAFVLTVALPAWWLGHLVLLGRPAGMDAPPQRAEQATSQGTDAALAAPPPVDWYPLGRVLVWIAGFAALTTTAALLTLGSDASAITDALRRGLVRIAKVQDGASGDRASEVVDAFVAIAPAAAATIAMATLTLNMWLAAKVTAASGRLRRPWPDVRTTTLPPMALVATMIALALSFVGGLPAMLAQIITACLMMTYALAGFAVLHTITLTMRNRGLWLGSVYAIVLMFVWPVITVVGIGIADAVFGFRQRYLQRHTPPPLTPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS06</accession>
    <name>Q3SS06_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1674</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1674"/>
    <dbReference type="EnsemblBacteria" id="ABA04935">
        <property type="protein sequence ID" value="ABA04935"/>
        <property type="gene ID" value="Nwi_1674"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1674"/>
    <dbReference type="eggNOG" id="ENOG502ZR1Z">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1509470_0_0_5"/>
    <dbReference type="OMA" id="ADGMCPG"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229238" evidence="1">
        <location>
            <begin position="28"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="185" mass="19429" checksum="AFCB39B4D1EC68FD" modified="2005-10-11" version="1">MRQPRFRRRALIPLLLVAPLVSGCGTASDMFSTGLLSKDADWFSRSGRLFIKNVSVEAPPLTPDKPVTPDDLISADGLCPGMAPPIGMGESSALADSAGDSPATSALTGTVALGHTECDVTRGLGAAPDNVSLSSNPRGDRVAVLTFLKGPRAGIYTFTAGRLSSIEQAPESAVRQKPERRAKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS14</accession>
    <name>Q3SS14_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidase_S15 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1666</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314926.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1666"/>
    <dbReference type="ESTHER" id="nitwn-q3ss14">
        <property type="family name" value="Atu1826-like"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04927">
        <property type="protein sequence ID" value="ABA04927"/>
        <property type="gene ID" value="Nwi_1666"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1666"/>
    <dbReference type="eggNOG" id="COG2945">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086287_0_0_5"/>
    <dbReference type="OMA" id="MDWARPQ"/>
    <dbReference type="OrthoDB" id="1090356at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000383">
        <property type="entry name" value="Xaa-Pro-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02129">
        <property type="entry name" value="Peptidase_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_S15" evidence="1">
        <location>
            <begin position="17"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="215" mass="23908" checksum="FD53EF0154E73C06" modified="2005-10-11" version="1">MPEVIFTGPAGRLEGRYHPAKQKNAPIAMVLHPHPQFRGSMNHPIVYQVYYAFVARGFSVLRFNFRGVGRSQGSFDHGTGELSDAAAALDWAQTINPEARACWVAGFSFGAWIGMQLLMRRPEVEGFISIAPEPNRYDFSFLAPCPSSGLIVHGEKDIVAPAKDVTTLVEKLKTQKGIVIDQQTIPGANHFFEDKMEPLMETVTSYLDMRLANIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS56</accession>
    <name>Q3SS56_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1481</name>
        <name evidence="2" type="ordered locus">Nwi_1624</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="2">Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314749.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1481"/>
    <dbReference type="EnsemblBacteria" id="ABA04742">
        <property type="protein sequence ID" value="ABA04742"/>
        <property type="gene ID" value="Nwi_1481"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04885">
        <property type="protein sequence ID" value="ABA04885"/>
        <property type="gene ID" value="Nwi_1624"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1481"/>
    <dbReference type="KEGG" id="nwi:Nwi_1624"/>
    <dbReference type="eggNOG" id="ENOG5032EKQ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148545_0_0_5"/>
    <dbReference type="OMA" id="VIVKSHA"/>
    <dbReference type="OrthoDB" id="1539049at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="130" mass="13346" checksum="B1126351AD541AAF" modified="2005-10-11" version="1">MRDIASNIGTVQALAPAVQSATIKGETVDTAGFESCAFVINTGATAGSPAPSFTAKLQESDTTTDGDFTDVDATHLVGTLPDSLEESSTYKQSYIGHRKYARVVVTKNSGTSIAAGAVAVLGHARSRPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSI6</accession>
    <name>Q3SSI6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1494</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314761.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04755">
        <property type="protein sequence ID" value="ABA04755"/>
        <property type="gene ID" value="Nwi_1494"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1494"/>
    <dbReference type="HOGENOM" id="CLU_2480206_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="87" mass="9325" checksum="23861AA2A1ECCEFA" modified="2005-10-11" version="1">MKVLPFKRPSAKEKYRPDPANGEVYATEFFECSYIDGNEVQTPAGFAVVHESKSGGSAGIQRGFETMEAAKIAAVAEAVRLNAVLCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSP0</accession>
    <name>Q3SSP0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cyclase/dehydrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1440</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribosome association toxin RatA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314709.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1440"/>
    <dbReference type="EnsemblBacteria" id="ABA04701">
        <property type="protein sequence ID" value="ABA04701"/>
        <property type="gene ID" value="Nwi_1440"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1440"/>
    <dbReference type="eggNOG" id="COG2867">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079653_3_0_5"/>
    <dbReference type="OMA" id="YIDGPFK"/>
    <dbReference type="OrthoDB" id="1691158at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048039">
        <property type="term" value="F:ubiquinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045333">
        <property type="term" value="P:cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07813">
        <property type="entry name" value="COQ10p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044996">
        <property type="entry name" value="COQ10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005031">
        <property type="entry name" value="COQ10_START"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12901">
        <property type="entry name" value="PTHR12901"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03364">
        <property type="entry name" value="Polyketide_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polyketide_cyc" evidence="2">
        <location>
            <begin position="11"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="155" mass="17653" checksum="732F1727591E06FD" modified="2005-10-11" version="1">MPSFSSKRRVRHSASEMFDLVADVERYPDFVPLCQSLKVRQRTPAADGKEVVVADMTVSFKLVSESFTSKVTLDRPNLKIVVEYLRGPFSHMENRWTFEPKSDQGCDVGFFITYEFKSRMLAMLMGAMFDTAFQRFAAAFEKRAAAVYGPPKART</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSQ6</accession>
    <name>Q3SSQ6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TsaA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_1424</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the tRNA methyltransferase O family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1424"/>
    <dbReference type="EnsemblBacteria" id="ABA04685">
        <property type="protein sequence ID" value="ABA04685"/>
        <property type="gene ID" value="Nwi_1424"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1424"/>
    <dbReference type="eggNOG" id="COG1720">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013458_2_0_5"/>
    <dbReference type="OMA" id="VCRLEIF"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09281">
        <property type="entry name" value="UPF0066"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023370">
        <property type="entry name" value="TrmO-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040372">
        <property type="entry name" value="YaeB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036413">
        <property type="entry name" value="YaeB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036414">
        <property type="entry name" value="YaeB_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12818">
        <property type="entry name" value="PTHR12818"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01980">
        <property type="entry name" value="TrmO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118196">
        <property type="entry name" value="SSF118196"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00104">
        <property type="entry name" value="tRNA_TsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51668">
        <property type="entry name" value="TSAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="TsaA-like" evidence="4">
        <location>
            <begin position="37"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00033753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="173" mass="18960" checksum="FE16E37D0C6FE833" modified="2005-10-11" version="1">MTGPDKEKRGPRAMGQENEVRAGEVVVEPPSPTDAGLVFVGRVRTPWRTRAEAPRQGQPDGPVCRLEIFDPWKPALQGLERYESIEVIYWLHLSRRDIVRQVPKSSGSSHGTFALRSPVRPNPIGTSIVKLVGIEGSTVLVRGLDCLDETPLLDIKPIRCQFASAPKGDGETG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSV2</accession>
    <name>RS8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsH</name>
        <name type="ordered locus">Nwi_1378</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Contacts proteins S5 and S12.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314652.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSV2"/>
    <dbReference type="SMR" id="Q3SSV2"/>
    <dbReference type="STRING" id="323098.Nwi_1378"/>
    <dbReference type="EnsemblBacteria" id="ABA04639">
        <property type="protein sequence ID" value="ABA04639"/>
        <property type="gene ID" value="Nwi_1378"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1378"/>
    <dbReference type="eggNOG" id="COG0096">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098428_0_0_5"/>
    <dbReference type="OMA" id="FDQGYIL"/>
    <dbReference type="OrthoDB" id="1692188at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01302_B">
        <property type="entry name" value="Ribosomal_S8_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_S8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_S8_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11758">
        <property type="entry name" value="PTHR11758"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="SSF56047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00053">
        <property type="entry name" value="RIBOSOMAL_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000225876" description="30S ribosomal protein S8">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01302"/>
        </source>
    </evidence>
    <sequence length="132" mass="14640" checksum="9EA33CFEA55591C9" modified="2005-10-11" version="1">MSTHDPISDLITRIRNAQMRSKPKVSTPGSKMRANVLEVLKSEGYIRGYASVEHSSGRSELEIELKYFDGEPVIREIERVSRPGRRVYASVKNLPRVNNGLGISVLSTPKGIMADHEARDANVGGEVLFTVF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-05-25" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSX1</accession>
    <name>RS12_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">30S ribosomal protein S12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rpsL</name>
        <name type="ordered locus">Nwi_1359</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">With S4 and S5 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the 30S ribosomal subunit. Contacts proteins S8 and S17. May interact with IF1 in the 30S initiation complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314637.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSX1"/>
    <dbReference type="SMR" id="Q3SSX1"/>
    <dbReference type="STRING" id="323098.Nwi_1359"/>
    <dbReference type="EnsemblBacteria" id="ABA04620">
        <property type="protein sequence ID" value="ABA04620"/>
        <property type="gene ID" value="Nwi_1359"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1359"/>
    <dbReference type="eggNOG" id="COG0048">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104295_1_2_5"/>
    <dbReference type="OMA" id="SPALEKC"/>
    <dbReference type="OrthoDB" id="1707228at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03368">
        <property type="entry name" value="Ribosomal_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00403_B">
        <property type="entry name" value="Ribosomal_S12_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006032">
        <property type="entry name" value="Ribosomal_S12/S23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005679">
        <property type="entry name" value="Ribosomal_S12_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11652">
        <property type="entry name" value="PTHR11652"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
        <property type="entry name" value="Ribosom_S12_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002133">
        <property type="entry name" value="Ribosomal_S12/S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01034">
        <property type="entry name" value="RIBOSOMALS12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00981">
        <property type="entry name" value="rpsL_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00055">
        <property type="entry name" value="RIBOSOMAL_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0488">Methylation</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000226398" description="30S ribosomal protein S12">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="modified residue" description="3-methylthioaspartic acid" evidence="1">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00403"/>
        </source>
    </evidence>
    <sequence length="123" mass="13862" checksum="60315D7E92D2B924" modified="2005-10-11" version="1">MPTINQLIASPRVLQKSRKKVPALQQSPQKRGVCTRVYTTTPKKPNSALRKVAKVRLTNGFEVIGYIPGEGHNLQEHSVVMIRGGRVKDLPGVRYHILRGVLDTQGVKNRKQRRSKYGAKRPK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-11-28" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST95</accession>
    <name>PSD_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphatidylserine decarboxylase proenzyme</fullName>
            <ecNumber evidence="1">4.1.1.65</ecNumber>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName evidence="1">Phosphatidylserine decarboxylase alpha chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="1">Phosphatidylserine decarboxylase beta chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="1" type="primary">psd</name>
        <name type="ordered locus">Nwi_1234</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine + H(+) = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + CO2</text>
            <dbReference type="Rhea" id="RHEA:20828"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="EC" id="4.1.1.65"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
        <text evidence="1">Binds 1 pyruvoyl group covalently per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from CDP-diacylglycerol: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of a large membrane-associated beta subunit and a small pyruvoyl-containing alpha subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="1">Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The post-translation cleavage follows an unusual pathway, termed non-hydrolytic serinolysis, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl prosthetic group on the alpha chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphatidylserine decarboxylase family. PSD-A subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.65" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314525.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ST95"/>
    <dbReference type="STRING" id="323098.Nwi_1234"/>
    <dbReference type="EnsemblBacteria" id="ABA04496">
        <property type="protein sequence ID" value="ABA04496"/>
        <property type="gene ID" value="Nwi_1234"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1234"/>
    <dbReference type="eggNOG" id="COG0688">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072492_0_0_5"/>
    <dbReference type="OMA" id="VSIFMSP"/>
    <dbReference type="OrthoDB" id="891720at2"/>
    <dbReference type="UniPathway" id="UPA00558">
        <property type="reaction ID" value="UER00616"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004609">
        <property type="term" value="F:phosphatidylserine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00664">
        <property type="entry name" value="PS_decarb_PSD_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003817">
        <property type="entry name" value="PS_Dcarbxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033175">
        <property type="entry name" value="PSD-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35809">
        <property type="entry name" value="PTHR35809"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02666">
        <property type="entry name" value="PS_Dcarbxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00164">
        <property type="entry name" value="PS_decarb_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0210">Decarboxylase</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-0670">Pyruvate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0865">Zymogen</keyword>
    <feature type="chain" id="PRO_0000262235" description="Phosphatidylserine decarboxylase beta chain" evidence="1">
        <location>
            <begin position="1"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000262236" description="Phosphatidylserine decarboxylase alpha chain" evidence="1">
        <location>
            <begin position="206"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via pyruvic acid" evidence="1">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage (non-hydrolytic); by autocatalysis" evidence="1">
        <location>
            <begin position="205"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser); by autocatalysis" evidence="1">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00664"/>
        </source>
    </evidence>
    <sequence length="247" mass="27063" checksum="080E4DAC3531803F" modified="2005-10-11" version="1">MPIDRQTLLSIAKSVRSGARSVRAQISPIHPKGYPFVGGFALATIILFWIWSPLGWIGTLLTIWCALFFRNPARVTPIREGLVVAPADGRISMIAPVVPPAELELGELPMVRISIFMSVFNCHVNRSPVAGRIERIVYRPGKFINAELDKASEDNERNALVISTPNNGLVGVIQIAGLIARRIVTFVHDGQTVETGERFGLIRFGSRLDVFLPEGTQVLVSEGQTTIAGETVLADFQQTDGRTFRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STA9</accession>
    <name>Q3STA9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">Phosphoglycolate phosphatase</fullName>
            <shortName evidence="10">PGP</shortName>
            <shortName evidence="10">PGPase</shortName>
            <ecNumber evidence="5 10">3.1.3.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">gph</name>
        <name evidence="11" type="ordered locus">Nwi_1220</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Specifically catalyzes the dephosphorylation of 2-phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>2-phosphoglycolate + H2O = glycolate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14369"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29805"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58033"/>
            <dbReference type="EC" id="3.1.3.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Organic acid metabolism; glycolate biosynthesis; glycolate from 2-phosphoglycolate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.18" evidence="5 10"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1220"/>
    <dbReference type="EnsemblBacteria" id="ABA04482">
        <property type="protein sequence ID" value="ABA04482"/>
        <property type="gene ID" value="Nwi_1220"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1220"/>
    <dbReference type="eggNOG" id="COG0546">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_19_1_5"/>
    <dbReference type="OMA" id="YLCGKFG"/>
    <dbReference type="UniPathway" id="UPA00865">
        <property type="reaction ID" value="UER00834"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008967">
        <property type="term" value="F:phosphoglycolate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046295">
        <property type="term" value="P:glycolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00495">
        <property type="entry name" value="GPH_hydrolase_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006346">
        <property type="entry name" value="PGPase/MupP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037512">
        <property type="entry name" value="PGPase_prok"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01449">
        <property type="entry name" value="PGP_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7 10 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="5"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="5"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA04482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="225" mass="23986" checksum="D951FB5617960C4C" modified="2005-10-11" version="1">MVVFDLDGTLVDTAPDLAAALNFVLDREGLRKVSLPTARSMIGAGVRRMIERGLEHEDRSATPDEVTRMMGDFVDYYTAHIADESRPFEGLIEALDELSARGFRFAVCTNKLEALAKLLLDQLGLSPRFSAICGGDTFGVAKPDPAILRQTIARAGGNPRAAIMVGDAGPDIGVARRAGIPVIGVTFGYTEVPVAELKPDRVISHMRELPEAIAALQAATLNAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STG7</accession>
    <name>Q3STG7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1162</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1162"/>
    <dbReference type="EnsemblBacteria" id="ABA04424">
        <property type="protein sequence ID" value="ABA04424"/>
        <property type="gene ID" value="Nwi_1162"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1162"/>
    <dbReference type="eggNOG" id="ENOG5033ECW">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2356886_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="96" mass="11006" checksum="1083CFED118BC43A" modified="2005-10-11" version="1">MRRSRAATAWSEPSLTRKGRKGRKTAMSDLIRIFSDRGDLAHLALFLWASAASAGLLFALRELSAASRRFDDFVRELQRFNRTAHRRAENIKEDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STJ2</accession>
    <name>Q3STJ2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Flagellar biosynthetic protein FlhB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">flhB</name>
        <name evidence="15" type="ordered locus">Nwi_1137</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">Required for formation of the rod structure in the basal body of the flagellar apparatus. Together with FliI and FliH, may constitute the export apparatus of flagellin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the type III secretion exporter family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314430.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1137"/>
    <dbReference type="MEROPS" id="N06.A01"/>
    <dbReference type="EnsemblBacteria" id="ABA04399">
        <property type="protein sequence ID" value="ABA04399"/>
        <property type="gene ID" value="Nwi_1137"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1137"/>
    <dbReference type="eggNOG" id="COG1377">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041013_1_2_5"/>
    <dbReference type="OMA" id="DEYKQQE"/>
    <dbReference type="OrthoDB" id="1424100at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044780">
        <property type="term" value="P:bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1690.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006136">
        <property type="entry name" value="FlhB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006135">
        <property type="entry name" value="T3SS_substrate_exporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029025">
        <property type="entry name" value="T3SS_substrate_exporter_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30531">
        <property type="entry name" value="PTHR30531"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01312">
        <property type="entry name" value="Bac_export_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00950">
        <property type="entry name" value="TYPE3IMSPROT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160544">
        <property type="entry name" value="SSF160544"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00328">
        <property type="entry name" value="flhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword evidence="11 13" id="KW-1006">Bacterial flagellum protein export</keyword>
    <keyword evidence="5 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="15" id="KW-0969">Cilium</keyword>
    <keyword evidence="15" id="KW-0282">Flagellum</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="190"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="10"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU364091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABA04399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="359" mass="39611" checksum="30B8EBDD304C4578" modified="2005-10-11" version="1">MADESDDTEKTEDPTQKRLDDALERGDVAKSQEVNTWFVIAGATLVLSTFSGSIGSGFELPLRNLIANSWQIRTDGPGLLALAEQIGYRLAAALGVPLLLLMMAAVAGNVIQHRFVWSGETLKPKLSKISMVSGAKRIFGKQAAANFAKGLFKVLALGAVMTAVLWPERSRLDAMVRLDPNALLGITKSLTLQLLGAVIVMLALVAIADYLFQYRQWFERQKMSLREMKEEFKQSEGDPHIKARIRQLRHVRMKKRMMAAVPQASVIITNPTHYSVALSYERGMSAPVCVAKGVDNIALKIREIAREHDIPVVENVPLARALYAAVEIDDEVPVEHYHAVAEIIGYVMGLKRGLSGRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STN6</accession>
    <name>Q3STN6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04355">
        <property type="protein sequence ID" value="ABA04355"/>
        <property type="gene ID" value="Nwi_1093"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1093"/>
    <dbReference type="eggNOG" id="ENOG5032V6Z">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119030_0_0_5"/>
    <dbReference type="OMA" id="WQIGGFI"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="195" mass="21942" checksum="A520EAE28DA974D5" modified="2005-10-11" version="1">MSPPPEMPPPDVGYAYRASAIGSAHRFALEDDGLSWRASRRTGRWRYEDIVAVRMSYRPMSMQSHRFRTEISHQDGGRIIVMSTTWRTLALMDAQSNDYRTFIVELHRRLAAHGARAMLAGGLNPALYFAGMSVLTLLSVAMTALLMRAIVTGEWAGALFIVGLSALFGWQIGRFMRRNRPRAYTLDALPRDLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STR8</accession>
    <name>Q3STR8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">DNA repair protein RecN</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4 5">Recombination protein N</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_1061</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">May be involved in recombinational repair of damaged DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the RecN family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314357.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1061"/>
    <dbReference type="EnsemblBacteria" id="ABA04323">
        <property type="protein sequence ID" value="ABA04323"/>
        <property type="gene ID" value="Nwi_1061"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1061"/>
    <dbReference type="eggNOG" id="COG0497">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018297_3_1_5"/>
    <dbReference type="OMA" id="QVICVTH"/>
    <dbReference type="OrthoDB" id="1074831at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004604">
        <property type="entry name" value="DNA_recomb/repair_RecN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11059">
        <property type="entry name" value="PTHR11059"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003128">
        <property type="entry name" value="RecN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00634">
        <property type="entry name" value="recN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC_N" evidence="6">
        <location>
            <begin position="14"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00033408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR003128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="561" mass="59826" checksum="0537D9B39371CB50" modified="2005-10-11" version="1">MLARLSIRDIVLIERLDIEFASGLAVLTGETGAGKSILLDAFALALGGRGDAGLVRHGAEQGQVTATFDVPKNHPACAMLAANDIENSISEESGEMILRRVQLADGRTRAFINDQAISVQTLKAVGATLVEIHGQHDERALVDASTHRRLLDAFAGLDTEVSALEVLWEARRRARVALEDHRAGVERAAQEAEYLRHASEELTQLAPQEGEETALAERRAAMMAGEKIAADLREARDVVSGHGSPASVLSAAVRRLERRAASSRQLVEPAVRSIDAAINALEEADQHLAAALAASDFDPAELERIEERLFALRAASRKYATPVEGLAALAAKYAAQVALIDAGADRLTVLEKEAADADKRYAAAAAKLSVARKKAADKLDKTVNVELAPLKLERARFSTHIDSDPSTPGPQGFDRVEFWVQTNPGTRPGPLMKVASGGELSRFLLALKVVLSDKGSAPTLVFDEIDTGVGGAVADAIGARLSRLACKVQVMAVTHAPQVAVRADQHLLISKDALDRGKRVATRVATLAEDHRREEIARMLAGAEITAEARAAADRLLKAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STT4</accession>
    <name>Q3STT4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Penicillin-binding protein, transpeptidase</fullName>
            <ecNumber evidence="5">2.4.1.129</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_1045</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.1.129" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314341.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1045"/>
    <dbReference type="EnsemblBacteria" id="ABA04307">
        <property type="protein sequence ID" value="ABA04307"/>
        <property type="gene ID" value="Nwi_1045"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1045"/>
    <dbReference type="eggNOG" id="COG0768">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009289_6_2_5"/>
    <dbReference type="OMA" id="HGKEEYY"/>
    <dbReference type="OrthoDB" id="213680at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008658">
        <property type="term" value="F:penicillin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008955">
        <property type="term" value="F:peptidoglycan glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005311">
        <property type="entry name" value="PBP_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036138">
        <property type="entry name" value="PBP_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001460">
        <property type="entry name" value="PCN-bd_Tpept"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03717">
        <property type="entry name" value="PBP_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00905">
        <property type="entry name" value="Transpeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56519">
        <property type="entry name" value="SSF56519"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="1 2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="36"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="PBP_dimer" evidence="4">
        <location>
            <begin position="77"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Transpeptidase" evidence="3">
        <location>
            <begin position="251"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="579" mass="64239" checksum="103045A3CEA83F36" modified="2005-10-11" version="1">MIGQNRLPPALWSQRLIRNLLYGRNVDRAAKARVRVGLVIILFALIYTVVAARLVMFAAFPDDRGGRRTASQDAIATARPDIIDRNGQVLATDIKSPSLFGEPRRLIDRDEAVELLTATLPDLETSEVRQRLGTRKGFVWLKREITPAQQKEIHSLGIPGIGFLRENKRVYPSGREVAHLIGLVNIDNQGIAGMEKWLDSNGLADLHRAGFATDRLQKPVELSIDLRVEHALRDELLKAKDHYRAKAASGIVSNVRTGEIVAMVSLPDFDPNNPKEAHDPDRINRLTTGVYEMGSTFKAFTLAMALDSGRYGLNSMWDARAPLRYGRFTIHDDHSMGRFINMKEVFTYSSNIGAARIALAQGVEAHKAFLRRMGQMDRLRTELPESASPILPRKWGELNTVTIAFGHGMAVAPLQAVMGIDALVNGGWLIPPTFLKRTEEEAMAVARRVIKQETSDKMRFLLRLNAEVGTARKADVKGYYVGGKTGTSEKVINGRYAKKRVLNSFTAILPADNPRYQILVMLDEPQPLKETHGFITSGWNAVPTGGKVIARIAPLLGVQPRFDLPPSERLILAASRATQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STT7</accession>
    <name>Q3STT7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-acetylmuramoyl-L-alanine amidase, family 2</fullName>
            <ecNumber evidence="2">3.5.1.28</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1042</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.1.28" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1042"/>
    <dbReference type="EnsemblBacteria" id="ABA04304">
        <property type="protein sequence ID" value="ABA04304"/>
        <property type="gene ID" value="Nwi_1042"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1042"/>
    <dbReference type="eggNOG" id="COG3023">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049290_2_2_5"/>
    <dbReference type="OMA" id="DIKPTRV"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008745">
        <property type="term" value="F:N-acetylmuramoyl-L-alanine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06583">
        <property type="entry name" value="PGRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.101.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036505">
        <property type="entry name" value="Amidase/PGRP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002502">
        <property type="entry name" value="Amidase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036366">
        <property type="entry name" value="PGBDSf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01510">
        <property type="entry name" value="Amidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00644">
        <property type="entry name" value="Ami_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="SSF47090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55846">
        <property type="entry name" value="SSF55846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetylmuramoyl-L-alanine amidase" evidence="1">
        <location>
            <begin position="40"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="283" mass="30651" checksum="A8E8D42E1C97B854" modified="2005-10-11" version="1">MFASVSPDIKRRRPLPVPKAIVGMPTFVPDSSLVSDTVPSLNFGDRAKGRQPDMIVLHYTGMPDVEGALARLCTSGTEVSAHYVVLEDGRVVQCVQESKRAWHAGVASWAGEEDINSCSIGIEIVNRGHDWGYPAFPLRQVAAVIALCRGIIHRRDIPAHRIVGHSDVAPARKKDPGEKFPWHSLADSGVGHWIRPAPIVAGEVLKTGSEGDDVRALQQSLARYGYGIKATGKFDTATMEVVTAFQRHFRPAKVDGIADQSTLTTLHALLDSLPAQKASNGIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STU1</accession>
    <name>Q3STU1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1038</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314334.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04300">
        <property type="protein sequence ID" value="ABA04300"/>
        <property type="gene ID" value="Nwi_1038"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1038"/>
    <dbReference type="HOGENOM" id="CLU_2001448_0_0_5"/>
    <dbReference type="OMA" id="SFFWLGF"/>
    <dbReference type="OrthoDB" id="2154023at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="127" mass="13990" checksum="AF30068E477462AF" modified="2005-10-11" version="1">MNLMLTGIFGLAGLVLSYGFSVPPELTAFRLIAPVGVMAGYQAGQLVGRLVRPRPKRFLILSVGILFCFYFTFAYIQLRASSPSDIANLSVLFGLSFFWLGFFLPFTRAPNQMKTLLSKALAKRSGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STW8</accession>
    <name>Q3STW8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Butyryl-CoA:acetate CoA transferase</fullName>
            <ecNumber evidence="3">2.8.3.8</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1011</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 3-oxoacid CoA-transferase subunit B family.</text>
    </comment>
    <dbReference type="EC" id="2.8.3.8" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314311.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1011"/>
    <dbReference type="EnsemblBacteria" id="ABA04273">
        <property type="protein sequence ID" value="ABA04273"/>
        <property type="gene ID" value="Nwi_1011"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1011"/>
    <dbReference type="eggNOG" id="COG2057">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019942_4_1_5"/>
    <dbReference type="OMA" id="VAMMHTN"/>
    <dbReference type="OrthoDB" id="1380130at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008775">
        <property type="term" value="F:acetate CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012791">
        <property type="entry name" value="3-oxoacid_CoA-transf_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004165">
        <property type="entry name" value="CoA_trans_fam_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13707">
        <property type="entry name" value="PTHR13707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01144">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00882">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02428">
        <property type="entry name" value="pcaJ_scoB_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="216" mass="23168" checksum="257C379E71DCD696" modified="2005-10-11" version="1">MAWTREQMAARAAKELRDGYYVNLGIGIPTLVSNFIPDNIDVQLQSENGMLGMGPFPYEGEEDPDLINAGKQTVTELPTTSYFSSADSFGMVRGGHIDLSILGAMQVAQNGDLANWMIPGKMVKGMGGAMDLVAGVKRVLVVMEHSAKDAPKLLKRCTLPLTGERVVDMVVTDLAVFTIDKHGDGGMRLIELAGGVTLDEVRARTEAEFGVALPNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU13</accession>
    <name>Q3SU13_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0966</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314269.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0966"/>
    <dbReference type="EnsemblBacteria" id="ABA04228">
        <property type="protein sequence ID" value="ABA04228"/>
        <property type="gene ID" value="Nwi_0966"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0966"/>
    <dbReference type="eggNOG" id="ENOG5033ZZE">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1119256_0_0_5"/>
    <dbReference type="OMA" id="DPYVQHD"/>
    <dbReference type="OrthoDB" id="1511354at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="260" mass="27701" checksum="3C05195450174D9F" modified="2005-10-11" version="1">MCDYSLHHVSSRPAQVGDQLVTMELARSSARGFAAVGEIGARLVVHDDAPQMAVCLLPGTELAFDAAVEYDRALSPKVFGVRVFGKARVNYTVASFRQVNLDDPYVQHDALEFPNGEIVKINRLIEGQTATVLQLPAVLPGNDLDHEHAHAGSIAPLHASRLVLNRGLPESGAASILVPNLGVATLWDALRGMAVSLRVSLMQAFRRLTARKPDLHSALPVTDSHSIVPVIASPRTSANPTSDKSVSKTAVKASAELPSA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-01-09" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU34</accession>
    <name>SELO_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein adenylyltransferase SelO</fullName>
            <ecNumber evidence="1">2.7.7.-</ecNumber>
            <ecNumber evidence="1">2.7.7.n1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">selO</name>
        <name type="ordered locus">Nwi_0945</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of adenosine 5'-monophosphate (AMP) to Ser, Thr or Tyr residues of target proteins (AMPylation).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-tyrosyl-[protein] = diphosphate + O-(5'-adenylyl)-L-tyrosyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:54288"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:13846"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:83624"/>
            <dbReference type="EC" id="2.7.7.n1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:54289"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-threonyl-[protein] = 3-O-(5'-adenylyl)-L-threonyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:54292"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13847"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:138113"/>
            <dbReference type="EC" id="2.7.7.n1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:54293"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-seryl-[protein] = 3-O-(5'-adenylyl)-L-seryl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:58120"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:15073"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:142516"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:58121"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SELO family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="1"/>
    <dbReference type="EC" id="2.7.7.n1" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314248.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SU34"/>
    <dbReference type="SMR" id="Q3SU34"/>
    <dbReference type="STRING" id="323098.Nwi_0945"/>
    <dbReference type="EnsemblBacteria" id="ABA04207">
        <property type="protein sequence ID" value="ABA04207"/>
        <property type="gene ID" value="Nwi_0945"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0945"/>
    <dbReference type="eggNOG" id="COG0397">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010245_4_1_5"/>
    <dbReference type="OMA" id="YGPYGWL"/>
    <dbReference type="OrthoDB" id="130048at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00692">
        <property type="entry name" value="SelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003846">
        <property type="entry name" value="SelO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32057">
        <property type="entry name" value="PTHR32057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02696">
        <property type="entry name" value="SelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000271837" description="Protein adenylyltransferase SelO">
        <location>
            <begin position="1"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="102"/>
            <end position="105"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="137"/>
            <end position="138"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00692"/>
        </source>
    </evidence>
    <sequence length="505" mass="55069" checksum="5BF240924A8D06DD" modified="2005-10-11" version="1">MSQLAEKSCVTPADAAAPIPFDNTYARMPEAFYERVKPAAVAAPRLLRVNDALARQLRIDPAFLKSPQGVAVLSGNEIAPGSDPIAQAYAGHQFGSFVPQLGDGRAILLGEVVDAAGKRFDIQLKGSGRTRFSRRGDGRAAIGPVIREYIVSEAMAALGIPTTRSLAVVLTGEQVMRERVLPGGILTRVASSHLRVGTFQYFAARGDVENLRALADYAIARHYPDVCSAGDPYLAFFDAVVATLARLSARWMLVGFIHGVLNTDNTAIAGETIDYGPCAFIDAYRPDKVFSSIDQFGRYAFENQPAAVAWNLARFAEALLPLIADGGAKAIECANASIAAFDDHFNAAYLSGMRRKLGLVREMEGDLELASDLLTRMSENGADFTLTFRALCGAADDTKGDAEVRGLFADPSAFDQWTERWRQRLSLEGQSAEVRRTDMLSVNPMFIPRNHRIEAAIRDAEAGRFETFHELVEVLSHPFDDQPKFAGYAKPPRPEEEVRQTFCGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU49</accession>
    <name>Q3SU49_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NfeD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0930</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314233.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0930"/>
    <dbReference type="EnsemblBacteria" id="ABA04192">
        <property type="protein sequence ID" value="ABA04192"/>
        <property type="gene ID" value="Nwi_0930"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0930"/>
    <dbReference type="eggNOG" id="COG1585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116732_4_3_5"/>
    <dbReference type="OMA" id="PGVFLFW"/>
    <dbReference type="OrthoDB" id="1951939at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002810">
        <property type="entry name" value="NfeD-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01957">
        <property type="entry name" value="NfeD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="NfeD" evidence="3">
        <location>
            <begin position="60"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="148" mass="15931" checksum="6764C33DD65D92ED" modified="2005-10-11" version="1">MIDLLASLGNWNWLILGLVLVGLELLTPGVFLFWLGLAAFLVGALSFVVDPTWQTQLLMFAVFAAAAVPLWRRMARRESSGAASPFLNRRAEALVGRVFTLERPIVDGTGTVRIDDTIWRVAGPDAPAGSRVKVVKADGASLTVATEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU98</accession>
    <name>Q3SU98_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TonB-dependent receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0879</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TonB-dependent receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0879"/>
    <dbReference type="EnsemblBacteria" id="ABA04143">
        <property type="protein sequence ID" value="ABA04143"/>
        <property type="gene ID" value="Nwi_0879"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0879"/>
    <dbReference type="eggNOG" id="COG4774">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008287_2_1_5"/>
    <dbReference type="OMA" id="HYWANET"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015891">
        <property type="term" value="P:siderophore transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030149">
        <property type="entry name" value="FcuA/FatA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039423">
        <property type="entry name" value="TonB-dep_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000531">
        <property type="entry name" value="TonB-dep_rcpt_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036942">
        <property type="entry name" value="TonB_rcpt_b-brl_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32552">
        <property type="entry name" value="PTHR32552"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32552:SF82">
        <property type="entry name" value="PTHR32552:SF82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00593">
        <property type="entry name" value="TonB_dep_Rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TonB_dep_Rec" evidence="2">
        <location>
            <begin position="17"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="378" mass="41478" checksum="9F46AE785B2DE12F" modified="2005-10-11" version="1">MTTAATTTFSLDTVDSNQAALNGRFETSSIKHDLFLATNGFLWNRYTPFRRGAITVGSASLSNPSIFSEPNFPDFKDRYRSVYTFQQSVTLGDTIALNERWSILAAVSNSWIETSNYNNAGGVTGGYSASGWSPLGSIMFKPGENVTTYVTYANSLQQGDSAPAGTVNQGEALAPFRSNQYELGAKFDFGRFNLNAALYRIERPYAYTGSDNVFSMKRTQINRGVELMASGAINDEWTVFGGMTFIDPRLHNMGSPQTEGRRILGLSQFVMNCLVEYHVPSVQGLTFTANVNYGTNRPTDYANTSFVPDYAVLNLGMRYAQLIAGKPVTFRFDVFNVTDLHYWANETPAAQNGYNATGSATGILGAPRTIRASLQVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUA0</accession>
    <name>Q3SUA0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0877</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0877"/>
    <dbReference type="EnsemblBacteria" id="ABA04141">
        <property type="protein sequence ID" value="ABA04141"/>
        <property type="gene ID" value="Nwi_0877"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0877"/>
    <dbReference type="eggNOG" id="COG0741">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1459872_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="185" mass="20855" checksum="6C0C4014F8237F3C" modified="2005-10-11" version="1">MIADADQIARDVGCDQDGEPDGPTGTPRTPERSPSSMALRGFARNTSRKPGKERGTRTRSCCQVVPAIQCWLLRCDRDGLVRRAVIDLADRPNAIDGPFPNPMPGDQCHPREEVGYNVGPGRVKEWIARYGDPRDPSVDAVDWVERIPFKENRIYVQRVMENLQIYRSQFRTTPHHTIEADMRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUG2</accession>
    <name>Q3SUG2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0814</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314127.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0814"/>
    <dbReference type="EnsemblBacteria" id="ABA04079">
        <property type="protein sequence ID" value="ABA04079"/>
        <property type="gene ID" value="Nwi_0814"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0814"/>
    <dbReference type="eggNOG" id="ENOG5031REJ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1194160_0_0_5"/>
    <dbReference type="OMA" id="HYDAKAW"/>
    <dbReference type="OrthoDB" id="1289168at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="234" mass="24990" checksum="A9BE9C752D39D362" modified="2005-10-11" version="1">MTPPIVREHGGVSVIRDDLFPGGTKARFMPVLFEGAEEAVYASPAEGGAQTALATVAKDLGKRATIFVAARAKLHPRTLEAARLGAKVVPVRPGYLSVVQARAKDYAKTSGARLVPFGVDLPEAITRLADAALATGLDPDELWCAAGSGVLARALDKAWPRARRHVVQVGRELAREDVANATIHVYPRPFSDVAKAISPFPSDPHYDAKAWETMTARKGPGRVVFWNVAGPARP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUG3</accession>
    <name>Q3SUG3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0813</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314126.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0813"/>
    <dbReference type="EnsemblBacteria" id="ABA04078">
        <property type="protein sequence ID" value="ABA04078"/>
        <property type="gene ID" value="Nwi_0813"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0813"/>
    <dbReference type="eggNOG" id="ENOG50341W4">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1720430_0_0_5"/>
    <dbReference type="OrthoDB" id="1986945at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="152" mass="16525" checksum="E7D233352D97BC71" modified="2005-10-11" version="1">MTTEATTYDKKFNAQRGAQRTGLKPGEFEVFKTPDGRFGWRAMATDTDGQPPIKTTESELATSPTSPKPGKRKAIIEQAQAGALPAAPDFSKPTHARFRAKLAKLVTLVEASDVEGLKAIEINPVSTSPKAMARYRDLALIAIEARRRQSAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUH1</accession>
    <name>Q3SUH1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0805</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314118.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0805"/>
    <dbReference type="EnsemblBacteria" id="ABA04070">
        <property type="protein sequence ID" value="ABA04070"/>
        <property type="gene ID" value="Nwi_0805"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0805"/>
    <dbReference type="eggNOG" id="ENOG5033GBA">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2261583_0_0_5"/>
    <dbReference type="OMA" id="IARVAIW"/>
    <dbReference type="OrthoDB" id="1784507at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="105" mass="10906" checksum="B3D0AA51CC769526" modified="2005-10-11" version="1">MTNAFIAAIDALFADPNIGEDAVWKAGGVGPGVAVRIIRKSPDRLAEFGESRAVLPTLTLDIRRSQSATIAEGDLIVIGAETFKIIGEPMGDALALVAACEAVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUK1</accession>
    <name>Q3SUK1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0775</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04040">
        <property type="protein sequence ID" value="ABA04040"/>
        <property type="gene ID" value="Nwi_0775"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0775"/>
    <dbReference type="HOGENOM" id="CLU_2845393_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="65" mass="7587" checksum="DB3F6C33EDF8A94F" modified="2005-10-11" version="1">MIRINYDVPVILIDLGNNHVLYSALIIAVRKKTHSRLNLIYTAKRFSRHSLLQRLIPFPISIFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUP8</accession>
    <name>Q3SUP8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0727</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0727"/>
    <dbReference type="EnsemblBacteria" id="ABA03993">
        <property type="protein sequence ID" value="ABA03993"/>
        <property type="gene ID" value="Nwi_0727"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0727"/>
    <dbReference type="eggNOG" id="ENOG5032B39">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_146726_0_0_5"/>
    <dbReference type="OMA" id="YIRQQVF"/>
    <dbReference type="OrthoDB" id="1984511at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="149" mass="16152" checksum="14484C475712E338" modified="2005-10-11" version="1">MPLSADFDRVAILAAEGGRQNAVRSPPKKRLPPFSIRLSPEERARLAVEAAGAPLGAYIKARVLGTVPVERKRRKVLTIKDREALAQALALLGRSHLSSNLNQLAYAANIGSLLVTPETEADLRAALADVRELRRLLLTALGFRPEGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUP9</accession>
    <name>Q3SUP9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ferric siderophore receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0726</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TonB-dependent receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0726"/>
    <dbReference type="EnsemblBacteria" id="ABA03992">
        <property type="protein sequence ID" value="ABA03992"/>
        <property type="gene ID" value="Nwi_0726"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0726"/>
    <dbReference type="eggNOG" id="COG4773">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136778_1_1_5"/>
    <dbReference type="OMA" id="NAGYYIQ"/>
    <dbReference type="OrthoDB" id="169533at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039423">
        <property type="entry name" value="TonB-dep_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000531">
        <property type="entry name" value="TonB-dep_rcpt_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036942">
        <property type="entry name" value="TonB_rcpt_b-brl_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32552">
        <property type="entry name" value="PTHR32552"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00593">
        <property type="entry name" value="TonB_dep_Rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TonB_dep_Rec" evidence="2">
        <location>
            <begin position="3"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="179" mass="19743" checksum="5F00F86DDE45FC4F" modified="2005-10-11" version="1">MNLRITAAIYDLRRKNVTTRDPAHPFFSIQTGEVTARGFEFEAVTSLTSGLSLTGAYAYNDARVTESNDANLGMRPIRTPQHLASVWANYTIHDGALNGLTLGGGVRYVGASAGDFMNTFYAPAYTVVDAMARYDIDSWRFSLNVTNLFDTQYVAGCGYIGQCNVGRVRTVLGRVSYRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUT5</accession>
    <name>Q3SUT5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Coenzyme PQQ synthesis D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0690</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; pyrroloquinoline quinone biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314012.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0690"/>
    <dbReference type="EnsemblBacteria" id="ABA03956">
        <property type="protein sequence ID" value="ABA03956"/>
        <property type="gene ID" value="Nwi_0690"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0690"/>
    <dbReference type="eggNOG" id="COG0535">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_163864_0_0_5"/>
    <dbReference type="OMA" id="WVILAPE"/>
    <dbReference type="OrthoDB" id="2008640at2"/>
    <dbReference type="UniPathway" id="UPA00539"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018189">
        <property type="term" value="P:pyrroloquinoline quinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008792">
        <property type="entry name" value="PQQD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022479">
        <property type="entry name" value="PqqD_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041881">
        <property type="entry name" value="PqqD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05402">
        <property type="entry name" value="PqqD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03859">
        <property type="entry name" value="PQQ_PqqD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0884">PQQ biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="100" mass="11374" checksum="899D967A46863046" modified="2005-10-11" version="1">MIPNTARPEIDGGTVVHLARGVRLRKDTVRNQTVLLAPERAIALDEIAVAIIKEIDGHRNLDRIADDLSRRFEAPKEQVLRDIIPFIREFINRRTLEVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUX7</accession>
    <name>Q3SUX7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NUDIX hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0648</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313974.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0648"/>
    <dbReference type="EnsemblBacteria" id="ABA03914">
        <property type="protein sequence ID" value="ABA03914"/>
        <property type="gene ID" value="Nwi_0648"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0648"/>
    <dbReference type="eggNOG" id="COG0494">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093822_0_0_5"/>
    <dbReference type="OMA" id="WISEYRY"/>
    <dbReference type="OrthoDB" id="1424483at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="1">
        <location>
            <begin position="79"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="261" mass="28914" checksum="4BAD16FC42CF81C4" modified="2005-10-11" version="1">MLNVRLLQSEPRVTLGAAAPHIDPDIEARVDEIWQREKELRSQEIYNGRLFSVDYCDARTIVGWISEYRYFLAQRREPSLHAALKIKPLAVTGTLSCPDGILFGRRSDRSEMDAGCWELVPSGGVEDTAVGSAGQVSLERCILIELEEETGIRASDLSAPSRAVALVEDPQSHVTDVGIMLATACSAAQIQRRFAALENREYSELEIIAFAEVQAFRRDRARSLSAVSAAVLDTLMQPGVCSGFLSNTFEVTQDPHYKRLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUZ4</accession>
    <name>Q3SUZ4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent Clp protease adapter protein ClpS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">clpS</name>
        <name evidence="3" type="ordered locus">Nwi_0630</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the modulation of the specificity of the ClpAP-mediated ATP-dependent protein degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Binds to the N-terminal domain of the chaperone ClpA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ClpS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313957.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0630"/>
    <dbReference type="EnsemblBacteria" id="ABA03897">
        <property type="protein sequence ID" value="ABA03897"/>
        <property type="gene ID" value="Nwi_0630"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0630"/>
    <dbReference type="eggNOG" id="COG2127">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134358_3_0_5"/>
    <dbReference type="OMA" id="TEDQAYK"/>
    <dbReference type="OrthoDB" id="1829294at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00302">
        <property type="entry name" value="ClpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022935">
        <property type="entry name" value="ClpS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003769">
        <property type="entry name" value="ClpS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014719">
        <property type="entry name" value="Ribosomal_L7/12_C/ClpS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33473">
        <property type="entry name" value="PTHR33473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02617">
        <property type="entry name" value="ClpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54736">
        <property type="entry name" value="SSF54736"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ClpS" evidence="2">
        <location>
            <begin position="18"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="101" mass="11299" checksum="2A659680F8B69560" modified="2005-10-11" version="1">MSDIAIKPRIKAETKIERPRLYKVILHNDDFTPREFVVAVLMAEFNMTDGQAHKVMITADRLGSCVVGVFARDIAETKATRATDAGRSMGFPLLFTTEPEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV10</accession>
    <name>Q3SV10_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0614</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0614"/>
    <dbReference type="EnsemblBacteria" id="ABA03881">
        <property type="protein sequence ID" value="ABA03881"/>
        <property type="gene ID" value="Nwi_0614"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0614"/>
    <dbReference type="eggNOG" id="COG4446">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068029_0_0_5"/>
    <dbReference type="OMA" id="RYGQHDL"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010865">
        <property type="entry name" value="DUF1499"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07386">
        <property type="entry name" value="DUF1499"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="317" mass="34878" checksum="A2A3C3E3D785BFE8" modified="2005-10-11" version="1">MESGSSLCLREHSFCSKRYRRSRRRRAAATNRFMARRFSARYQTEPFSALATWARRLAVFSAVATVVSVAAVRFGILESKPALATLLGALACAGLSVLLALAGLVALWRDGSRGIGRILTALLLNVLVLAYPAYLAVRHRNLPPIHDITTDPVNPPHFEALARLRGMDGANSAVYAGLYSAEQQRAAYPAIEPVELDLTAQQAFDIVRRLVTRRKWLVIDERPPQPPRQSGHIEAVARTPVMGFREDVAIRIMPTEDGSRIDIRSSSRYFEHDLGSNAARVSSLIDDINIAAENVVDKAVKEPPVPVKPPARGAVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV14</accession>
    <name>Q3SV14_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ATP-grasp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0610</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313937.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0610"/>
    <dbReference type="EnsemblBacteria" id="ABA03877">
        <property type="protein sequence ID" value="ABA03877"/>
        <property type="gene ID" value="Nwi_0610"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0610"/>
    <dbReference type="eggNOG" id="COG0189">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055286_0_1_5"/>
    <dbReference type="OMA" id="CPMTFQE"/>
    <dbReference type="OrthoDB" id="937913at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="2">
        <location>
            <begin position="133"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="324" mass="36580" checksum="7F5EF983A912B58C" modified="2005-10-11" version="1">MSETSPILIITAKLDTHADAVISELKARGQPFFRLNTDDFHTEYKTVLRSGDRSVTFEDIWGRSVRFPHDVHAIWHRKPIESNNPPGVEECGTADMIQGETLELLNSLSCFHDRPWYNNPESNRVSQRKFPQLCLASELGFRVPRTLITNDPGEARRFAAQTQGSILCKAMKEAGYSTPNGSYFIFSRQVGQDEFDAHADRIALCPTLLQEYIEKAYELRVTVIGDVVFACRLDSQADEAARIDWRMVTPDRIAHSMVTLDNKVEAALKAMMRRFGLRFGAFDLIVTPEGETVFVELNPNGQWYWIELTTGAPMASAMADLLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV17</accession>
    <name>Q3SV17_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">3,4-dihydroxy-2-butanone 4-phosphate synthase</fullName>
            <ecNumber evidence="6">4.1.99.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_0607</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-ribulose 5-phosphate = (2S)-2-hydroxy-3-oxobutyl phosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18457"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="4.1.99.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the C-terminal section; belongs to the GTP cyclohydrolase II family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the DHBP synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.12" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313934.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0607"/>
    <dbReference type="EnsemblBacteria" id="ABA03874">
        <property type="protein sequence ID" value="ABA03874"/>
        <property type="gene ID" value="Nwi_0607"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0607"/>
    <dbReference type="eggNOG" id="COG0108">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0807">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020273_1_2_5"/>
    <dbReference type="OMA" id="ECRGLIC"/>
    <dbReference type="OrthoDB" id="900513at2"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00399"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008686">
        <property type="term" value="F:3,4-dihydroxy-2-butanone-4-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000422">
        <property type="entry name" value="DHBP_synthase_RibB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032677">
        <property type="entry name" value="GTP_cyclohydro_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036144">
        <property type="entry name" value="RibA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00926">
        <property type="entry name" value="DHBP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00925">
        <property type="entry name" value="GTP_cyclohydro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142695">
        <property type="entry name" value="SSF142695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="SSF55821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00506">
        <property type="entry name" value="ribB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0686">Riboflavin biosynthesis</keyword>
    <feature type="domain" description="GTP_cyclohydro2" evidence="10">
        <location>
            <begin position="207"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA03874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="358" mass="38815" checksum="6AA4D769C36E28BD" modified="2005-10-11" version="1">MPHPIPEILQAFANGEIVVVTDDEDREGEGDLIVAASLCTAEKMAFVIRHTSGIVCAPITMDDAQRLRLDPMVAHNESNHTTAFTVSIDYKPDGGTGISAEERASCCRALANPNSGASDFARPGHIFPLIARNGGVLLRAGHTEAAVDLCRLASLPPVGVISELMNDDGTVMKGEQVARFADAHGLRHVTIADIIAYRQAREKLIERVSEFAIDSPIGPLQGYAYRSPFDSIAHVAFVYNGIGDGRNVLTRFHKSNIVRDIFAGPKRMENVLEHFRRDGRGVLVYLRDGAAGVPVAPLPEEGSAEADRNRQWREVGVGAQILRDLGVTSIRHLTSSAYDYKGLSGFGIEIVSNEHLET</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV51</accession>
    <name>Q3SV51_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0573</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313901.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0573"/>
    <dbReference type="EnsemblBacteria" id="ABA03840">
        <property type="protein sequence ID" value="ABA03840"/>
        <property type="gene ID" value="Nwi_0573"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0573"/>
    <dbReference type="eggNOG" id="COG0419">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015405_0_0_5"/>
    <dbReference type="OMA" id="YLWEIAL"/>
    <dbReference type="OrthoDB" id="169528at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012683">
        <property type="entry name" value="CHP02302_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13779">
        <property type="entry name" value="DUF4175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02302">
        <property type="entry name" value="aProt_lowcomp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="641"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="691"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="742"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="506"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="641"/>
            <end position="664"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="828" mass="90178" checksum="D20153C40A40FA52" modified="2005-10-11" version="1">MSGVTPDPSRQDDDPRALAGVELTRALRRARWVIAWERGWPHLARLLCVGGLFLAISWAGAWLVLPAWVRAVALAAFVLLAVAAAVPAVRFRWPSREDGLNRLDRGAGLRHRPVTALADTLATRDPVAQALWQAQRARTLTAIKRLRAGLPSPRLPLHDRWALRALVMVLMVATFIAAGGERTERVAAAFDRNGALTSAHVRVDAWVAPPPYTGKPPIILSATGKDANPSDTALPVPAGSTLIVRSSGGSLDVALSGGVTEKAVADKPAEGASERHFIIAADGAAQVRAPLGQPRWTFSAIADRAPAISLAKRPERQARGSLQMSYRLEDDYGVTEAHAAFSARPASAVNDGTAARPLFDPPQFALVLPNARPRSGIGQTVQDLSESPYAGAEVTLTLTARDDAGNEGRSEPLDLRLPERVFTKPLARALIEQRRILALDANQNRQVHTALGALLIAPEVFTPRAGEYLGLYSVTKQLEEARIDAQLREVVASLWGLAVSIEDGNITDVEKALRAAQDALKEALERRASDEEIRKLTENLRAALDNYLRQLAEQMRNNPEQLARPLDPNGKVMRPQDLDNMIERMERLSRSGDKEAARQLLDQLQQMLENLQMAQPGQGEGAGEDLQQSLNELGDMIRKQQQLRDKTYKQGMDSRRDDDDGQERGGQNGMGGLQQDQKALHDRLKTLQQELARRGMGSSQPDDDLGQADSAMGQAGGRLGEGNADGAVDAQGRALDALRKGARSLAEAMQQGEDGSGGRAGRQSGGQTDPLGRPLRGREFGNETTVRIPGEIDVQRVRRILEELRRRLADPSRPKIELDYIDRLLKDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV74</accession>
    <name>Q3SV74_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313878.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0550"/>
    <dbReference type="EnsemblBacteria" id="ABA03817">
        <property type="protein sequence ID" value="ABA03817"/>
        <property type="gene ID" value="Nwi_0550"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0550"/>
    <dbReference type="eggNOG" id="ENOG50339FM">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_938995_0_0_5"/>
    <dbReference type="OMA" id="HTHVATE"/>
    <dbReference type="OrthoDB" id="1263902at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="291" mass="30674" checksum="392CF8A786B4A51B" modified="2005-10-11" version="1">MNEAEQQRALSLDRGLFFINYKSAEDSVSPPRVIVAPAKGHERHMEIILHPDATEPTLYKPNSGLVVRVNMPATLQVQVQPTKPGGSRSAIVRVEQIQAGGIPSATDSDSGMGPHVATEGLKVLGHVAGRGDIVVGPNAWIAGPAAPSRVEGVALEWPDKPLSVDIRYAVQLANGQTGSGRMVPLGAYAGTRGRALPLTGVVLEMSGTDELRFVAEAGFLNAPTLRAVGRRVVLSGPTNREPLVGIRIGIERNATAEVAATPAPPPSRKPSGGSPRVRVFRSQQRQIGAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV83</accession>
    <name>Q3SV83_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glycosyl transferase, family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0541</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313869.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0541"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03808">
        <property type="protein sequence ID" value="ABA03808"/>
        <property type="gene ID" value="Nwi_0541"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0541"/>
    <dbReference type="eggNOG" id="COG0463">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_660276_0_0_5"/>
    <dbReference type="OMA" id="QTIAFAH"/>
    <dbReference type="OrthoDB" id="1379602at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008166">
        <property type="entry name" value="Glyco_transf_92"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01697">
        <property type="entry name" value="Glyco_transf_92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="416" mass="47394" checksum="65F83365A84431C1" modified="2005-10-11" version="1">METETAALTRLGLDRGVEALTANEIEPRLKEFFSGRIANPNPEELMRSVKELEEQFSGYSEYQFARAAVLMQACDFTRAREILMRLAKETPSDPRILHRLAVTHLNIGSAQAAQDTYLAALAVAPASENLRREFAGLLRVMEAGKLYAGVSRKKHRLSMVCAIKNEADDLLEWLHFHKLAGVDHFYLYDNESTDETRAIIESFPWPDMITYHYVKGEFGQIRAFHHAIDSYRNSSEWCAFIDADEFLYPVEGGSIRDVLDGIPSAPAVAVQWLNFGSNGHDAKPDGLCIESFTRRAPDDFPDHYVMKSIVRPDAIVAYLHPHQSLVLGCYVQEDGSPVFPIGGRCSVPKRRKLAINHYYTKSRQQLLKKRERGRPLAAGDPERIRAMEFFMLRDRNDVEDRTILKFLPELKKLIHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV85</accession>
    <name>Q3SV85_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase, family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0539</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313867.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0539"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03806">
        <property type="protein sequence ID" value="ABA03806"/>
        <property type="gene ID" value="Nwi_0539"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0539"/>
    <dbReference type="eggNOG" id="COG1216">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_905636_0_0_5"/>
    <dbReference type="OMA" id="RYCHDLD"/>
    <dbReference type="OrthoDB" id="865276at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="1">
        <location>
            <begin position="4"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="307" mass="35798" checksum="DB584D1B3B2C840C" modified="2005-10-11" version="1">MLASVVIACYKHEAYIEECLETIYRQTFSDVELIIVDDHSPDRSFEIAQKILKQRSFAKRFVSCSLLKNPLNLGAPCTWNRALSLAKGEMIFFLNSDDKFSKDRIRIFANQYEREKLFFGFSAVEPIDESGKPVTTHQFPTRLRYAIDRSRITGSPLSWIFLDSQVAASSGNFVMSRDLLRKIGLLSDLKYCHDWEFALRAVTVVEPKYVDSDRYMYRLHSTNSFRSLSNVAERETKACMSSYCVNAIAQRPVNRVCLSPINFGDAFYNLIKLHPNFCYWVKQLYAPYHPDHRTVDWNEWTMPLMWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV90</accession>
    <name>Q3SV90_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransf_11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0534</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0534"/>
    <dbReference type="EnsemblBacteria" id="ABA03801">
        <property type="protein sequence ID" value="ABA03801"/>
        <property type="gene ID" value="Nwi_0534"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0534"/>
    <dbReference type="eggNOG" id="COG4627">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119382_0_0_5"/>
    <dbReference type="OMA" id="YAHEIFP"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="1">
        <location>
            <begin position="14"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="208" mass="22738" checksum="C4D57726B58CA8F6" modified="2005-10-11" version="1">MNPGIKRLLNAGSGPGSARRIARLVQERGLEELRFDIDPNVKPHVVGSILELETSFAPQSFDVIWSSHVLEHLYAHEIFPTLRQFHRVLKPDGFALIMSPDVTAVAHFIVEQGIAAVAYHSPAGPIRPLDMLYGHSRAIEEGHVHMAHRTGFTAERLGNLLLTAGFPTVSVAMQNFEVCALALMPEADDAAIKKTLLASGFNFQELAG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-05-05" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVJ5</accession>
    <name>ATPD_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP synthase subunit delta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP synthase F(1) sector subunit delta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">F-type ATPase subunit delta</fullName>
            <shortName evidence="1">F-ATPase subunit delta</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpH</name>
        <name type="ordered locus">Nwi_0429</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation.</text>
    </comment>
    <comment type="function">
        <text evidence="1">This protein is part of the stalk that links CF(0) to CF(1). It either transmits conformational changes from CF(0) to CF(1) or is implicated in proton conduction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">F-type ATPases have 2 components, F(1) - the catalytic core - and F(0) - the membrane proton channel. F(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). F(0) has three main subunits: a(1), b(2) and c(10-14). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. F(1) is attached to F(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase delta chain family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="ABA03696" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03696.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313760.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVJ5"/>
    <dbReference type="SMR" id="Q3SVJ5"/>
    <dbReference type="STRING" id="323098.Nwi_0429"/>
    <dbReference type="EnsemblBacteria" id="ABA03696">
        <property type="protein sequence ID" value="ABA03696"/>
        <property type="gene ID" value="Nwi_0429"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0429"/>
    <dbReference type="eggNOG" id="COG0712">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085114_0_1_5"/>
    <dbReference type="OrthoDB" id="1937493at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045261">
        <property type="term" value="C:proton-transporting ATP synthase complex, catalytic core F(1)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01416">
        <property type="entry name" value="ATP_synth_delta_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026015">
        <property type="entry name" value="ATP_synth_OSCP/delta_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020781">
        <property type="entry name" value="ATPase_OSCP/d_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000711">
        <property type="entry name" value="ATPase_OSCP/dsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11910">
        <property type="entry name" value="PTHR11910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00213">
        <property type="entry name" value="OSCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00125">
        <property type="entry name" value="ATPASEDELTA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47928">
        <property type="entry name" value="SSF47928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01145">
        <property type="entry name" value="ATP_synt_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00389">
        <property type="entry name" value="ATPASE_DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0139">CF(1)</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000371038" description="ATP synthase subunit delta">
        <location>
            <begin position="1"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="186" mass="19614" checksum="766B5DE2B30A4D83" modified="2009-05-05" version="2">MAAVDPSVSGVSGRYATALFELAREDKSIDAVKADLDKFDAMLAESPELVRLVRSPVFSADTQSKALAAVLSKAGIGGTTANFLKVLAANRRLFAVADVIRAFRALVAKFKGEATAEVTVAEKLNDKNLDALKAALKSVTGKDITLNVKVDPSIIGGLVVKLGSRMVDTSLRTKLNSIKHAMKEAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVL2</accession>
    <name>Q3SVL2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, CarD family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0412</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0412"/>
    <dbReference type="EnsemblBacteria" id="ABA03679">
        <property type="protein sequence ID" value="ABA03679"/>
        <property type="gene ID" value="Nwi_0412"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0412"/>
    <dbReference type="eggNOG" id="COG1329">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048259_0_1_5"/>
    <dbReference type="OMA" id="LTQRQGF"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036101">
        <property type="entry name" value="CarD-like/TRCF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003711">
        <property type="entry name" value="CarD-like/TRCF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042215">
        <property type="entry name" value="CarD-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02559">
        <property type="entry name" value="CarD_CdnL_TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01058">
        <property type="entry name" value="CarD_TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141259">
        <property type="entry name" value="SSF141259"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CarD_TRCF" evidence="2">
        <location>
            <begin position="95"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="283" mass="30026" checksum="6506D6A3DF197324" modified="2005-10-11" version="1">MPSKTARASANTATSKTAASKTVASASKTAASKAGSRTTASKIASAPKVGSRTSVKPTAKSAAKAAVASKPAVKPVARVGARVEEPKKVLTQRQGFKTGEFVVYPAHGVGQILAIEEQEIAGAKLELFVINFIKDKMTLRVPTAKVANVGMRKLSEPGLVKRALETLKGRARIKRTMWSRRAQEYEAKINSGDIVAIAEVVRDLYRSESQPEQSYSERQLYEAALDRLSREIAVVQHVTETEAVKEIEGQLAKSPRRGAKAEAGGEADGDLEAADGDDAAVAA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVL8</accession>
    <name>RL28_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L28</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpmB</name>
        <name type="ordered locus">Nwi_0406</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL28 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313737.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVL8"/>
    <dbReference type="SMR" id="Q3SVL8"/>
    <dbReference type="STRING" id="323098.Nwi_0406"/>
    <dbReference type="EnsemblBacteria" id="ABA03673">
        <property type="protein sequence ID" value="ABA03673"/>
        <property type="gene ID" value="Nwi_0406"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0406"/>
    <dbReference type="eggNOG" id="COG0227">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064548_4_2_5"/>
    <dbReference type="OMA" id="MARRCEL"/>
    <dbReference type="OrthoDB" id="2062238at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00373">
        <property type="entry name" value="Ribosomal_L28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034704">
        <property type="entry name" value="L28p-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026569">
        <property type="entry name" value="Ribo_L28/L24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037147">
        <property type="entry name" value="Ribo_L28/L24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001383">
        <property type="entry name" value="Ribosomal_L28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13528">
        <property type="entry name" value="PTHR13528"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00830">
        <property type="entry name" value="Ribosomal_L28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143800">
        <property type="entry name" value="SSF143800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00009">
        <property type="entry name" value="L28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_1000007289" description="50S ribosomal protein L28">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00373"/>
        </source>
    </evidence>
    <sequence length="97" mass="10856" checksum="EA4374E3794FCA56" modified="2005-10-11" version="1">MSRRCELTAKAPLVGHKVSHSNIKTKRRFLPNLVNVTFVSETLSSSVRFRVSTHALRSVDHRGGFDAFLLKARDSELSPKAIELKRQIQKKQAAKAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVT8</accession>
    <name>Q3SVT8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Methionine aminopeptidase</fullName>
            <shortName evidence="2">MAP</shortName>
            <shortName evidence="2">MetAP</shortName>
            <ecNumber evidence="2 3">3.4.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Peptidase M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">map</name>
        <name evidence="5" type="ordered locus">Nwi_0336</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="2 3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="2 3">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="2 3">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="2 3">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="2 3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313668.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0336"/>
    <dbReference type="EnsemblBacteria" id="ABA03603">
        <property type="protein sequence ID" value="ABA03603"/>
        <property type="gene ID" value="Nwi_0336"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0336"/>
    <dbReference type="eggNOG" id="COG0024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015857_0_0_5"/>
    <dbReference type="OMA" id="RGAESCY"/>
    <dbReference type="OrthoDB" id="1285136at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070084">
        <property type="term" value="P:protein initiator methionine removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01086">
        <property type="entry name" value="MetAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01974">
        <property type="entry name" value="MetAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002467">
        <property type="entry name" value="Pept_M24A_MAP1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="SSF55920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00500">
        <property type="entry name" value="met_pdase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00680">
        <property type="entry name" value="MAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 5" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 3" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M24" evidence="4">
        <location>
            <begin position="27"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="271" mass="29631" checksum="31FC97A33F6C9D6B" modified="2005-10-11" version="1">MSYVEASETPLRKTGQIKLHGPSGFAGMRNAGALTARCLDELADFVKPGVLTSQIDDLVREFAFSHGAYPATLMYRGYRYSTCTSVNHVVCHGMPGDRVLREGDIVNVDVTLIVDGWYGDSSRMYPVGPIARKAERLIDITYEAMMRGIAAVKPGATTGDIGHAIQSFVEPQGMSVVRDFCGHGLGRIFHDEPNIIHVGRPGDGVRLRPGMFFTIEPMINLGKPHVKILSDGWTAVTRDRSLSAQFEHSVGVTQTGVEIFTLSPRQLDKPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVW1</accession>
    <name>Q3SVW1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0313</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03580">
        <property type="protein sequence ID" value="ABA03580"/>
        <property type="gene ID" value="Nwi_0313"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0313"/>
    <dbReference type="HOGENOM" id="CLU_2586155_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="80" mass="8903" checksum="01DFE555130CEBEF" modified="2005-10-11" version="1">MSGRIFLRRTGIHFAGKRSRGGTHFENALDHDDSGSKRSRRINAIDFDILRSGRRAENRHVLARSGRSSLTLASVGQQSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVZ4</accession>
    <name>Q3SVZ4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0280</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313613.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03547">
        <property type="protein sequence ID" value="ABA03547"/>
        <property type="gene ID" value="Nwi_0280"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0280"/>
    <dbReference type="HOGENOM" id="CLU_2024288_0_0_5"/>
    <dbReference type="OMA" id="FKIDHKL"/>
    <dbReference type="OrthoDB" id="1998687at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229190" evidence="2">
        <location>
            <begin position="25"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="132" mass="14242" checksum="3079C54DD4198095" modified="2005-10-11" version="1">MILAKMILAGSLLAIVGAAAPAQATPPTQQTPSAETRKGLVTVVNRLNGTIAIREEKNDKSKDKDEEDKHKDKDKDKDNKDKAEAEGATTQFRIDLKLSESVHAGDKVKFSVKDGGDPKTGTDKTITKIEVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW06</accession>
    <name>Q3SW06_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Restriction endonuclease S subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_0268</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-I restriction system S methylase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313601.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0268"/>
    <dbReference type="REBASE" id="11313">
        <property type="entry name" value="S.NwiORF269P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03535">
        <property type="protein sequence ID" value="ABA03535"/>
        <property type="gene ID" value="Nwi_0268"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0268"/>
    <dbReference type="eggNOG" id="COG0732">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021095_10_1_5"/>
    <dbReference type="OMA" id="WIAKLIN"/>
    <dbReference type="OrthoDB" id="827072at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.220.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000055">
        <property type="entry name" value="Restrct_endonuc_typeI_TRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044946">
        <property type="entry name" value="Restrct_endonuc_typeI_TRD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01420">
        <property type="entry name" value="Methylase_S"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0680">Restriction system</keyword>
    <feature type="domain" description="Methylase_S" evidence="5">
        <location>
            <begin position="126"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Methylase_S" evidence="5">
        <location>
            <begin position="311"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="149"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA03535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="444" mass="49287" checksum="34327F6B7FFC3804" modified="2005-10-11" version="1">MSAAWPTVALGDLLRRSEHIIPLDPEATYKEVTVRINGKGVVERRQVQGVEIAANRRYQAKSGQFIISRIDARHGASGLIPDELDGAVVTNDFPLFDVAEDRLDAAFLGWMSKTASFVELCKRASEGTTNRVRLSEDRFKALSIPLPPLDEQRRIVARIEELAAKVKEARGLRAAAIEEVEAHWPAILRLAFDGKLVSLVPFKASAQEILKQAATFHANYQETKNNNAYPNKPQISDNGPYALPTGWCWTTLGSVLTHMVDCVNDTPNFSEVDTGLLGLKTTNIRPYRLDLQRRWYMTPGDFASWNRRQPPQAGDIVLTREAPVGNVCMLPEGISACLTQRLMLLRAENRVIQSRYLLHFLNSPCFTDQIAASGRGQTHPHIRVGDAPHFLLPLPPMEQQVKIVAELDALQSKLDSVKALQTETAAELDAMLPAILDKAFTGEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW27</accession>
    <name>MURA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UDP-N-acetylglucosamine 1-carboxyvinyltransferase</fullName>
            <ecNumber evidence="1">2.5.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Enoylpyruvate transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">UDP-N-acetylglucosamine enolpyruvyl transferase</fullName>
            <shortName evidence="1">EPT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">murA</name>
        <name type="ordered locus">Nwi_0246</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine</text>
            <dbReference type="Rhea" id="RHEA:18681"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:68483"/>
            <dbReference type="EC" id="2.5.1.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the EPSP synthase family. MurA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.7" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313580.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SW27"/>
    <dbReference type="SMR" id="Q3SW27"/>
    <dbReference type="STRING" id="323098.Nwi_0246"/>
    <dbReference type="EnsemblBacteria" id="ABA03514">
        <property type="protein sequence ID" value="ABA03514"/>
        <property type="gene ID" value="Nwi_0246"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0246"/>
    <dbReference type="eggNOG" id="COG0766">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027387_0_0_5"/>
    <dbReference type="OMA" id="CDPHRAT"/>
    <dbReference type="OrthoDB" id="537477at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008760">
        <property type="term" value="F:UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019277">
        <property type="term" value="P:UDP-N-acetylgalactosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01555">
        <property type="entry name" value="UdpNAET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00111">
        <property type="entry name" value="MurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001986">
        <property type="entry name" value="Enolpyruvate_Tfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036968">
        <property type="entry name" value="Enolpyruvate_Tfrase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005750">
        <property type="entry name" value="UDP_GlcNAc_COvinyl_MurA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00275">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="SSF55205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01072">
        <property type="entry name" value="murA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0133">Cell shape</keyword>
    <keyword id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword id="KW-0670">Pyruvate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000231226" description="UDP-N-acetylglucosamine 1-carboxyvinyltransferase">
        <location>
            <begin position="1"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="22"/>
            <end position="23"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="131"/>
            <end position="135"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="338"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="2-(S-cysteinyl)pyruvic acid O-phosphothioketal" evidence="1">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00111"/>
        </source>
    </evidence>
    <sequence length="429" mass="45062" checksum="E0E9F8E1DC029A2E" modified="2005-10-11" version="1">MDRIRIVGGNRLNGTIPISGAKNAALPLMIAALLTEETLILDNVPRLADVAQLQRILGNHGVDIMSAGKRPGDHEYQGQTLHISAASVIDTTAPYELVSKMRASFWVIAPLLARMHEARVSLPGGCAIGTRPVDLLILALEKLGARIAIDGGYVIASAPAGLRGAEVAFPKVTVSGTHVALMAATLASGTTVITNAACEPEIVDVADCLNKMGARVSGAGTPRIVIEGVGRLGGARHTVLPDRIETGTYAMAVAMTGGEVLLRGARPELLQAALDVLTEAGAEITPNNEGIRVARNGAGINPVTVSTAPFPGFPTDLQAQLMALMTRARGTSHITETIFENRFMHVQELARLGARIHLDGETATIEGIETLRGAPVMATDLRASVSLVIAGLAGEGETMVNRVYHLDRGFEQLEKKLSACGASIERIRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW28</accession>
    <name>Q3SW28_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0245</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03513">
        <property type="protein sequence ID" value="ABA03513"/>
        <property type="gene ID" value="Nwi_0245"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0245"/>
    <dbReference type="HOGENOM" id="CLU_2570360_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="81" mass="9294" checksum="FDC3402F7E5D1722" modified="2005-10-11" version="1">MSIKINSPAPADYKGRIRTFGQKMKIMTEKRDTDRDAENGGRRMRLRAALRENLKRRKSQSRGRGDQAASAPDPDPEHRDE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWE0</accession>
    <name>GLND_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Bifunctional uridylyltransferase/uridylyl-removing enzyme</fullName>
            <shortName evidence="1">UTase/UR</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Bifunctional [protein-PII] modification enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Bifunctional nitrogen sensor protein</fullName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName evidence="1">[Protein-PII] uridylyltransferase</fullName>
                <shortName evidence="1">PII uridylyltransferase</shortName>
                <shortName evidence="1">UTase</shortName>
                <ecNumber evidence="1">2.7.7.59</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="1">[Protein-PII]-UMP uridylyl-removing enzyme</fullName>
                <shortName evidence="1">UR</shortName>
                <ecNumber evidence="1">3.1.4.-</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="1" type="primary">glnD</name>
        <name type="ordered locus">Nwi_0133</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein-PII]-L-tyrosine + UTP = [protein-PII]-uridylyl-L-tyrosine + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13673"/>
            <dbReference type="Rhea" id="RHEA-COMP:12147"/>
            <dbReference type="Rhea" id="RHEA-COMP:12148"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:90602"/>
            <dbReference type="EC" id="2.7.7.59"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein-PII]-uridylyl-L-tyrosine + H2O = [protein-PII]-L-tyrosine + H(+) + UMP</text>
            <dbReference type="Rhea" id="RHEA:48600"/>
            <dbReference type="Rhea" id="RHEA-COMP:12147"/>
            <dbReference type="Rhea" id="RHEA-COMP:12148"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:90602"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GlnD family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.59" evidence="1"/>
    <dbReference type="EC" id="3.1.4.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313470.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWE0"/>
    <dbReference type="SMR" id="Q3SWE0"/>
    <dbReference type="STRING" id="323098.Nwi_0133"/>
    <dbReference type="PRIDE" id="Q3SWE0"/>
    <dbReference type="EnsemblBacteria" id="ABA03401">
        <property type="protein sequence ID" value="ABA03401"/>
        <property type="gene ID" value="Nwi_0133"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0133"/>
    <dbReference type="eggNOG" id="COG2844">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012833_1_0_5"/>
    <dbReference type="OMA" id="WIAKYVY"/>
    <dbReference type="OrthoDB" id="162558at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008773">
        <property type="term" value="F:[protein-PII] uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00277">
        <property type="entry name" value="PII_uridylyl_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013546">
        <property type="entry name" value="PII_UdlTrfase/GS_AdlTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010043">
        <property type="entry name" value="UTase/UR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47320">
        <property type="entry name" value="PTHR47320"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08335">
        <property type="entry name" value="GlnD_UR_UTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006288">
        <property type="entry name" value="PII_uridyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01693">
        <property type="entry name" value="UTase_glnD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000231682" description="Bifunctional uridylyltransferase/uridylyl-removing enzyme">
        <location>
            <begin position="1"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="domain" description="HD" evidence="2">
        <location>
            <begin position="498"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="domain" description="ACT 1" evidence="1">
        <location>
            <begin position="739"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="domain" description="ACT 2" evidence="1">
        <location>
            <begin position="849"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="region of interest" description="Uridylyltransferase">
        <location>
            <begin position="1"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Uridylyl-removing">
        <location>
            <begin position="383"/>
            <end position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01175"/>
        </source>
    </evidence>
    <sequence length="925" mass="104377" checksum="9B4D8076280C0070" modified="2005-10-11" version="1">MVLPTTKDATAAGAGFDTVRIIGDIDALAEKHAGHEDVFRSAVSRLLKAELAKVRDAAQAKLLRDRHGRHCAEWLCFVQDEIIRLSFSAATRHLYHSPIPSDGERMAVVATGGYGRGLMAPESDIDLLFILPYKQTAWGEQVAEAILYCLWDMGLNVGHATRSVNESIRQARRDMTVRTGILEARFLTGDRALYDELISRFDTEVVQGTAAEFVAAKLAEREERHHRAGQSRYLVEPNVKDGKGGLRDLHTLFWIAKYVYRVRESHELLRRNVFDVREYRTFRRCADFLWSVRCNLHFATGRAEERLSFDLQREIAVRLGYTSHPGMQDVERFMKHYFLTAKDVGDLTAILCAKLEDQQAKPAPVLSRAMSKPPGAEVRRVPDSDDFIIDNNRINLAAPDLFKRDPVNLIRLFRLAQKNNLAFHPDALRMVRRSRRLINAQLREDPESNRLFIEILTSNDAETVLRRMNETGVLGEFIRAFGKIVSMMQFNMYHHYTVDEHLIRCIGILQDIERGDNDEVALAGELMRTINPEHRPVIYIATLLHDVAKGRPEDHSIAGARVARRLCPRLGFNAADTELVAWLIEQHLTMSKVAQSRDLSDRKTIENFAAVVQSVERMKLLTILTTADIRGVGPGVWNGWKAQLLRTLYYETEPVLTGGFSEVNRAQRMAAAEAEFRAAFTEWSGHELNAYIARHYPAYWLKVDLEHKIRHARFLRASEQSGRKLNINVGFDEARGVTELTILAADHPWLLSIIAGACASAGANIVDAQIYTTTDGQALDTIAISREYERDEDEGRRAARIAEIIEQVLEGRLRLPDVMPSRAAGKRLRPFVVEPKVTINNQWSDRHTMIEVSGLDRPGLLFQLTTAISKLNLNIASAHVATFGERARDVFYVTDLLGARITAPTRQAAIKRALVHLLASGNTAE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWE7</accession>
    <name>HIS7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Imidazoleglycerol-phosphate dehydratase</fullName>
            <shortName evidence="1">IGPD</shortName>
            <ecNumber evidence="1">4.2.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisB</name>
        <name type="ordered locus">Nwi_0126</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:11040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="EC" id="4.2.1.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 6/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the imidazoleglycerol-phosphate dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.19" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313463.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWE7"/>
    <dbReference type="SMR" id="Q3SWE7"/>
    <dbReference type="STRING" id="323098.Nwi_0126"/>
    <dbReference type="EnsemblBacteria" id="ABA03394">
        <property type="protein sequence ID" value="ABA03394"/>
        <property type="gene ID" value="Nwi_0126"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0126"/>
    <dbReference type="eggNOG" id="COG0131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044308_3_0_5"/>
    <dbReference type="OMA" id="ARHGLFD"/>
    <dbReference type="OrthoDB" id="1278103at2"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00011"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004424">
        <property type="term" value="F:imidazoleglycerol-phosphate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07914">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00076">
        <property type="entry name" value="HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038494">
        <property type="entry name" value="IGPD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000807">
        <property type="entry name" value="ImidazoleglycerolP_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020565">
        <property type="entry name" value="ImidazoleglycerP_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23133">
        <property type="entry name" value="PTHR23133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00475">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00954">
        <property type="entry name" value="IGP_DEHYDRATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00955">
        <property type="entry name" value="IGP_DEHYDRATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000010314" description="Imidazoleglycerol-phosphate dehydratase">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00076"/>
        </source>
    </evidence>
    <sequence length="197" mass="21365" checksum="0923232733F62FF7" modified="2005-10-11" version="1">MRTASIKRKTKETDIEVAVNLDGSGVSEVATGIGFFDHMLDLLARHSRIDITVRADGDLHVDHHHTTEDVGIALGQAVKQALGSMVGISRYASVYMPMDETLSRVVIDISGRPVLVFKVGFPRDKIGEFDTELVREWFNAFAVNAGVTLHVETLYGENSHHIAESCFKGLARALRAAVAIDPRAANEVPSTKGQLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWQ1</accession>
    <name>Q3SWQ1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription termination/antitermination protein NusA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">nusA</name>
        <name evidence="9" type="ordered locus">Nwi_0022</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Participates in both transcription termination and antitermination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer. Binds directly to the core enzyme of the DNA-dependent RNA polymerase and to nascent RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the NusA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313361.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0022"/>
    <dbReference type="EnsemblBacteria" id="ABA03290">
        <property type="protein sequence ID" value="ABA03290"/>
        <property type="gene ID" value="Nwi_0022"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0022"/>
    <dbReference type="eggNOG" id="COG0195">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029242_2_6_5"/>
    <dbReference type="OMA" id="MDIDMSA"/>
    <dbReference type="OrthoDB" id="384865at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1480.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00945_B">
        <property type="entry name" value="NusA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010995">
        <property type="entry name" value="DNA_repair_Rad51/TF_NusA_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025249">
        <property type="entry name" value="KH_dom_NusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030842">
        <property type="entry name" value="NusA_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036555">
        <property type="entry name" value="NusA_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013735">
        <property type="entry name" value="TF_NusA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010214">
        <property type="entry name" value="Tscrpt_termin_fac_NusA_C_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010213">
        <property type="entry name" value="Tscrpt_termination_fac_NusA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22648">
        <property type="entry name" value="PTHR22648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13184">
        <property type="entry name" value="KH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08529">
        <property type="entry name" value="NusA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47794">
        <property type="entry name" value="SSF47794"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69705">
        <property type="entry name" value="SSF69705"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01953">
        <property type="entry name" value="NusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01954">
        <property type="entry name" value="nusA_Cterm_rpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0889">Transcription antitermination</keyword>
    <keyword evidence="5 7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 7" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="S1 motif" evidence="8">
        <location>
            <begin position="141"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA03290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="538" mass="59601" checksum="225857C39D870CC9" modified="2005-10-11" version="1">MAAVSANKLELLQIADAVAREKTIDRGIVIAAMEDAIAKAARARYGSETDVHAEIHPKTGQLQLTRHMLVVEQVENAANQISLKDAQRANPGAQIGDTIADTLPPLEYGRISAQSAKQVIVQKVREAERDRQYQEFKDRIGDIVNGIVKRVEYGSVIVDLGRGEAVIRRDEMLPREVFRNGDRVRAYIFDVRRETRGPQIFLSRTHPQFMVKLFTQEVPEIYDGIVEIKAVARDPGSRAKIGVVSRDSSVDPVGACVGMRGSRVQAVVNELQGEKIDIIPWSPDIATFVVNALAPAEVAKVVIDEDRERIEVVVPDTNNQLSLAIGRRGQNVRLASQLTGWDIDILTEQEESERRQADFENATRMFMETLNVDEVVGQLLASEGFTSVEELTLVDTREIAGIEGFDDETATELQNRAREYLEQLEAELENRRKELGVDDALKTVPGVTSKMLVKLGENEVRTIEDLAGCATDDLVGWTERKEGEAVKHAGYFDGIEISREDAEAIIMQARLSVGWINEADLAKPAEAEDIAAEDQPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN02</accession>
    <name>Q3SN02_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Toxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_3092</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the RelE toxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316252.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3092"/>
    <dbReference type="EnsemblBacteria" id="ABA06339">
        <property type="protein sequence ID" value="ABA06339"/>
        <property type="gene ID" value="Nwi_3092"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3092"/>
    <dbReference type="eggNOG" id="COG3668">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147162_3_1_5"/>
    <dbReference type="OMA" id="RHGHEDW"/>
    <dbReference type="OrthoDB" id="2036309at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2310.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028344">
        <property type="entry name" value="ParE1/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007712">
        <property type="entry name" value="RelE/ParE_toxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035093">
        <property type="entry name" value="RelE/ParE_toxin_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05016">
        <property type="entry name" value="ParE_toxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029218">
        <property type="entry name" value="ParE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-1277">Toxin-antitoxin system</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR029218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA06339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="97" mass="11110" checksum="DD5F1D63A9C020CB" modified="2005-10-11" version="1">MSFRLSLAAEDDIIGIAEEGVRLFGPAQARQYHDDLFAILDLIAANPRMARERHELSPPMRIHPFKAHLVIYRIEEDGVLIVRVRHGHEDWVSEGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN10</accession>
    <name>Q3SN10_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PilT protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_3084</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316246.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3084"/>
    <dbReference type="EnsemblBacteria" id="ABA06331">
        <property type="protein sequence ID" value="ABA06331"/>
        <property type="gene ID" value="Nwi_3084"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3084"/>
    <dbReference type="eggNOG" id="COG5611">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121449_0_0_5"/>
    <dbReference type="OMA" id="TNILVRY"/>
    <dbReference type="OrthoDB" id="1842663at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PINc" evidence="1">
        <location>
            <begin position="4"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="129" mass="14158" checksum="C9AF3D7885E57680" modified="2005-10-11" version="1">MLAIDTNLVVRYLTNDHPRQSPRARALIDGQAVFVAVTVMLEVDWVLRSAYGYRPADIARAMRSFAGLPTVSVEDPTVVAAALDLSENGMDFADALHLCKTTHCEGMASFDRKFVKAARAAGYDRVREA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN19</accession>
    <name>Q3SN19_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Membrane protein insertase YidC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 13">Foldase YidC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11 13">Membrane integrase YidC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Membrane protein YidC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">yidC</name>
        <name evidence="16" type="ordered locus">Nwi_3074</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Required for the insertion and/or proper folding and/or complex formation of integral membrane proteins into the membrane. Involved in integration of membrane proteins that insert both dependently and independently of the Sec translocase complex, as well as at least some lipoproteins. Aids folding of multispanning membrane proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with the Sec translocase complex via SecD. Specifically interacts with transmembrane segments of nascent integral membrane proteins during membrane integration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cell inner membrane</location>
            <topology evidence="13">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the OXA1/ALB3/YidC family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316238.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3074"/>
    <dbReference type="EnsemblBacteria" id="ABA06322">
        <property type="protein sequence ID" value="ABA06322"/>
        <property type="gene ID" value="Nwi_3074"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3074"/>
    <dbReference type="eggNOG" id="COG0706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016535_1_0_5"/>
    <dbReference type="OMA" id="INPVAGC"/>
    <dbReference type="OrthoDB" id="524437at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01810">
        <property type="entry name" value="YidC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019998">
        <property type="entry name" value="Membr_insert_YidC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028055">
        <property type="entry name" value="Membr_insert_YidC/Oxa1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028053">
        <property type="entry name" value="Membr_insert_YidC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001708">
        <property type="entry name" value="YidC/ALB3/OXA1/COX18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038210">
        <property type="entry name" value="YidC_periplasmic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038221">
        <property type="entry name" value="YidC_periplasmic_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428">
        <property type="entry name" value="PTHR12428"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02096">
        <property type="entry name" value="60KD_IMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14849">
        <property type="entry name" value="YidC_periplas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00701">
        <property type="entry name" value="60KDINNERMP"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01900">
        <property type="entry name" value="YIDCPROTEIN"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03593">
        <property type="entry name" value="yidC_nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03592">
        <property type="entry name" value="yidC_oxa1_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="5 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 13" id="KW-0143">Chaperone</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="383"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="446"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="512"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="552"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="domain" description="YidC_periplas" evidence="15">
        <location>
            <begin position="82"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="36"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF14849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ABA06322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="614" mass="68174" checksum="2E5D4AC3B40EC407" modified="2005-10-11" version="1">MSENRNTIIAIVLSALVLIAWQYFYNIPQMEKDRAAHQAQSELAKSQSKSQGEAAPNTAAPGQSAAGSAVVARDVAIASKPRVQINTPRLSGSISLTGARIDDLLLTKYRETVNPASPAIELFSPSGTASPYYAEFGWVPGTGSTVRVPDRSTLWQQEGAGALTPSTPVTLKYDNGEGLTFRRTVSVDDQYLFTIKDEVTNTGSAPVTLYPFALISRHGTPEISGYYILHEGLIGYLGDQKLQEYSYKNIDEAGSVTFNVTNGWLGITDKYWAAALLPDTSARLQARFSSDQAGAVRTYQTDYLEDARTIPAGGTGAVSTRLFAGAKEAGVVGLNFPLAELGGYNKQLGLNHFDLLIDWGWFYFITKPMFLALDFFFHVFGNFGVSILVVTVLIKLVFFPLANRSYASMAKMKAIQPQINALRELYPDDRMKLQQEMMEIYKKEKINPISGCLPVLLQIPVFFSLYKVLFVTIEMRHAPFFAWIKDLSAPDPTHIFNLFGLLPYDPSVVPLIGPYLAIGAWPIIMGVTMWFQMKLNPTPPDPTQKIIFDWMPVIFTFMLAAFPAGLVIYWAWNNTLSVIQQSYIMRRNGVKVELFDNVKSTFRRKSSDKPAKTT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-05-26" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN25</accession>
    <name>DAPE_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Succinyl-diaminopimelate desuccinylase</fullName>
            <shortName evidence="1">SDAP desuccinylase</shortName>
            <ecNumber evidence="1">3.5.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dapE</name>
        <name type="ordered locus">Nwi_3068</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the hydrolysis of N-succinyl-L,L-diaminopimelic acid (SDAP), forming succinate and LL-2,6-diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bacterial cell walls.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + N-succinyl-(2S,6S)-2,6-diaminoheptanedioate = (2S,6S)-2,6-diaminoheptanedioate + succinate</text>
            <dbReference type="Rhea" id="RHEA:22608"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57609"/>
            <dbReference type="ChEBI" id="CHEBI:58087"/>
            <dbReference type="EC" id="3.5.1.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) or Co(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route): step 3/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20A family. DapE subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.18" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316232.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SN25"/>
    <dbReference type="SMR" id="Q3SN25"/>
    <dbReference type="STRING" id="323098.Nwi_3068"/>
    <dbReference type="EnsemblBacteria" id="ABA06316">
        <property type="protein sequence ID" value="ABA06316"/>
        <property type="gene ID" value="Nwi_3068"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3068"/>
    <dbReference type="eggNOG" id="COG0624">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021802_4_0_5"/>
    <dbReference type="OMA" id="LNSHHDT"/>
    <dbReference type="OrthoDB" id="275025at2"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00021"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009014">
        <property type="term" value="F:succinyl-diaminopimelate desuccinylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01690">
        <property type="entry name" value="DapE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005941">
        <property type="entry name" value="DapE_proteobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01246">
        <property type="entry name" value="dapE_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0170">Cobalt</keyword>
    <keyword id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0457">Lysine biosynthesis</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000375629" description="Succinyl-diaminopimelate desuccinylase">
        <location>
            <begin position="1"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="74"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01690"/>
        </source>
    </evidence>
    <sequence length="389" mass="41309" checksum="377425B9FB1DDE42" modified="2005-10-11" version="1">MGNDALSIARDLIRCPSVTPADAGALGVLEPLLKNAGFDVHRITFSEPGVADIDNLYARIGSGRPHIAFAGHTDVVPPGDEAAWTRGAFSGDVVDGFLYGRGAVDMKGGVACGVAAALDYLAAHGGRPGKDGSGSISFLITGDEEDIAVNGTVKLLQWAAARGEKFDHCVLGEPSNVEELGDCIKIGRRGSQSGTLYVDGVQGHVAYPHRASNPIPEIAALITALVSEPLDQGSSQFQPSNLEFTSVDVGNPAANVIPGQARARFNIRFNDHHDQQSLRALVEARVAGASGNRVRTRIVWEPSNADVFLTEPGPFTDLVAAAVEDVTGRRPELSTGGGTSDARFIKDYCPVIEFGLVGRTMHQIDERVPIADLEKLTRVYRGVLDRYFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN39</accession>
    <name>Q3SN39_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_3053</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316218.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3053"/>
    <dbReference type="EnsemblBacteria" id="ABA06302">
        <property type="protein sequence ID" value="ABA06302"/>
        <property type="gene ID" value="Nwi_3053"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3053"/>
    <dbReference type="eggNOG" id="COG2271">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_59_9_5"/>
    <dbReference type="OMA" id="YGMTEVQ"/>
    <dbReference type="OrthoDB" id="1594866at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="104"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="144"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="290"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="347"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="379"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="12"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA06302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="414" mass="43519" checksum="551CC72F2F487A3E" modified="2005-10-11" version="1">MQKAQQHFHGPWIVAVAFVTLMGAFGLNLSGGQFFAPLAQEFGWGLAALSSAAALNMIVWGIFQPLTGRMIDRFGPKPVIVGSAALMGAAFLLSSTISTVWEFYLYYGVLAAIGFAGCGSMANSVLVARWYVRGRARMLARSAMGMNIGQLVFLPLTGWLIIVSGFRGAFLGLGVLMIVVIVPLVLFVAHSSPDKINQAPDGDELSTFTAPKSASLSQAVRSPDFWAATCGFVTCGYSLYLVVIHLPRFAVDLGANLATGGQVLGLAAGASAISMWTCGQLAGRAGKKNLLIGLYLVRALSLAFLAVSTEVWQLYAFALVYGLSSMPIIPLKTGLIGDLFGANAMGSILGTVWFLHQILAAIAVYLGGYLRVETGSYAAAFWSAAILLLIGAASTSLVRSPGGPVPLKPNPARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN42</accession>
    <name>Q3SN42_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC-type Fe3+-hydroxamate transport system periplasmic component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_3050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316215.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3050"/>
    <dbReference type="EnsemblBacteria" id="ABA06299">
        <property type="protein sequence ID" value="ABA06299"/>
        <property type="gene ID" value="Nwi_3050"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3050"/>
    <dbReference type="eggNOG" id="COG0614">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038034_8_0_5"/>
    <dbReference type="OMA" id="MDDKINF"/>
    <dbReference type="OrthoDB" id="1473468at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="1">
        <location>
            <begin position="47"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="299" mass="32296" checksum="41D8743CCD11C1B8" modified="2005-10-11" version="1">MSWLSRLHLFRNAIRAGIAARSGDLAAVALGFAIAMVAPAHAADLPRIASINLCTDQLLMTLADPAQIVGLSPYSRDAARSYAADEARNYRRLSGEVEDVLMLGPDVVVATPYTKHATRELLKQQGMRVDEFDVANSVEDVKDQIRRMGDLVRHPDRAEMQISRLDAAIARARKATARKRYQVLALSRRGWVSGADSLASALLSVAGLSNAANRLGIRYGGFAKLETIVAARPDLILISDGGNIAEDQGSALLLHPALEKMYPPSRRIIVPEDLTVCGGPMLADALERLVAELERIGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN76</accession>
    <name>Q3SN76_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative serine/threonine protein phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_3015</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3015"/>
    <dbReference type="EnsemblBacteria" id="ABA06265">
        <property type="protein sequence ID" value="ABA06265"/>
        <property type="gene ID" value="Nwi_3015"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3015"/>
    <dbReference type="eggNOG" id="COG0639">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2082337_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="1">
        <location>
            <begin position="5"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="117" mass="12601" checksum="90EE9772714FE26D" modified="2005-10-11" version="1">MSLTYVIPDIHGRFDLLSEALHGIHAHAAGNGGTLVMLGDYVDKGPDSDKVIARLRRGMPAGWTFVPLKGNHDAMMISACRDPATMTRWLKRGGDAVVESYGGDPLGRIDIQDSQAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN81</accession>
    <name>Q3SN81_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_3010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06260">
        <property type="protein sequence ID" value="ABA06260"/>
        <property type="gene ID" value="Nwi_3010"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3010"/>
    <dbReference type="HOGENOM" id="CLU_2437822_0_0_5"/>
    <dbReference type="OMA" id="HIDRWAN"/>
    <dbReference type="OrthoDB" id="2069881at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="96" mass="10548" checksum="56A90C2ABF33B4D0" modified="2005-10-11" version="1">MLTTMRVVASKQSSGERCSRSVPFQTGSNAMRVQRGLRELKSLKDRLAEFAAGVNKAAGEPSPNARNDEALRKRRLSDIAAHIDRWANSPGLQTPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNA4</accession>
    <name>Q3SNA4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cytochrome c, class I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2987</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316159.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2987"/>
    <dbReference type="EnsemblBacteria" id="ABA06237">
        <property type="protein sequence ID" value="ABA06237"/>
        <property type="gene ID" value="Nwi_2987"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2987"/>
    <dbReference type="eggNOG" id="COG3258">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052974_1_1_5"/>
    <dbReference type="OMA" id="FWPYATT"/>
    <dbReference type="OrthoDB" id="1469969at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13442">
        <property type="entry name" value="Cytochrome_CBB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229102" evidence="5">
        <location>
            <begin position="24"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="6">
        <location>
            <begin position="59"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA06237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="188" mass="19763" checksum="7A4F4D4D892BA520" modified="2005-10-11" version="1">MSMRNSWFGCVIGVCLFSGSAGAQSMVGHFGVGQTATADEAAKYFSIPPSGDGLPAGSGTAKAGVEVFASTCAACHSDKLQGNPAAGIGGDRLLGGRGSLAGKTPVKTVESYWPYATTLFDYIKRTMPFSAPGSLKDDEVYSLVAYILSEARIIKPSETIDAKSLPKVQMPNKDGFIPDARPELELYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNH5</accession>
    <name>Q3SNH5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2916</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2916"/>
    <dbReference type="EnsemblBacteria" id="ABA06166">
        <property type="protein sequence ID" value="ABA06166"/>
        <property type="gene ID" value="Nwi_2916"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2916"/>
    <dbReference type="eggNOG" id="COG0465">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1553683_0_0_5"/>
    <dbReference type="OMA" id="HHYRPMS"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="SSF140990"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="172" mass="18488" checksum="13AFF9FFDC8AE11F" modified="2005-10-11" version="1">MIAHHEAGHAVIARVLDIPVSYATLDALDGAGAVMTASSATHLAFDDLEAPEKYAIVALAGPQAQHHYRPMSLAAQNRACDGSWRVDRANATSLLAMLVLRQHGKPALPGSYVELDGPETEEFDRLFIRTTQKTADLVQENWPAIQRVAAALLSRPGLTEGDIDALIAARDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNS2</accession>
    <name>Q3SNS2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8 10">FAD:protein FMN transferase</fullName>
            <ecNumber evidence="2 8 10">2.7.1.180</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Flavin transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_2816</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Flavin transferase that catalyzes the transfer of the FMN moiety of FAD and its covalent binding to the hydroxyl group of a threonine residue in a target flavoprotein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8 10">
            <text>FAD + L-threonyl-[protein] = AMP + FMN-L-threonyl-[protein] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36847"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11061"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:74257"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.1.180"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="9">Magnesium. Can also use manganese.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 10">Belongs to the ApbE family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.180" evidence="2 8 10"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316014.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2816"/>
    <dbReference type="EnsemblBacteria" id="ABA06069">
        <property type="protein sequence ID" value="ABA06069"/>
        <property type="gene ID" value="Nwi_2816"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2816"/>
    <dbReference type="eggNOG" id="COG1477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044403_0_2_5"/>
    <dbReference type="OMA" id="MGTYYSI"/>
    <dbReference type="OrthoDB" id="2021063at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017013">
        <property type="term" value="P:protein flavinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024932">
        <property type="entry name" value="ApbE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003374">
        <property type="entry name" value="ApbE-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30040">
        <property type="entry name" value="PTHR30040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02424">
        <property type="entry name" value="ApbE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006268">
        <property type="entry name" value="ApbE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143631">
        <property type="entry name" value="SSF143631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-0274">FAD</keyword>
    <keyword evidence="4 8 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10 11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 8 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 10" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR006268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR006268-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA06069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="338" mass="36555" checksum="CE739F1A6E2C5FA1" modified="2005-10-11" version="1">MTAPVARRVAIPIIPEMPHAVSSAERIRELAGTTMGTTWTVKFVGSSARAQTLHRMIPLALERVIAQMSPWEGQSDISRFNRLALNRWQDLPPEFSRVMVSALRIAAESGGAYDPTMGALVDLWGFGPSGARASPPSPDEIAQRHRECGWRRLDLDAGARRMRRSSYGLLDLCGIAKGFAVDLVAETLRELGIHNALVEIGGELRGLGVKPDGSPWWVEIDRPARPTDKAAAEPILVALHQLAIATSGCERGFTRGADHFSHTLDPRTGWPIANGMMTATVLHPSCMEADGYATALMVLGPDAGVDFAVKHQLAALILFRQGDAVMERTTPALDAMLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNX5</accession>
    <name>Q3SNX5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sulfate adenylyltransferase subunit 2</fullName>
            <ecNumber evidence="3">2.7.7.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">ATP-sulfurylase small subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Sulfate adenylate transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_2763</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H(+) + sulfate = adenosine 5'-phosphosulfate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18133"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="EC" id="2.7.7.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PAPS reductase family. CysD subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.4" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2763"/>
    <dbReference type="EnsemblBacteria" id="ABA06016">
        <property type="protein sequence ID" value="ABA06016"/>
        <property type="gene ID" value="Nwi_2763"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2763"/>
    <dbReference type="eggNOG" id="COG0175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043026_0_0_5"/>
    <dbReference type="OMA" id="MMILAED"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004781">
        <property type="term" value="F:sulfate adenylyltransferase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019419">
        <property type="term" value="P:sulfate reduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01713">
        <property type="entry name" value="PAPS_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002500">
        <property type="entry name" value="PAPS_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011784">
        <property type="entry name" value="SO4_adenylTrfase_ssu"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01507">
        <property type="entry name" value="PAPS_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002936">
        <property type="entry name" value="CysDAde_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAPS_reduct" evidence="7">
        <location>
            <begin position="29"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA06016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="268" mass="30502" checksum="57D732864EABACB9" modified="2005-10-11" version="1">MQPGMDHLDALEAQSIYIFREAFARLKKLALLWSLGKDSNVMIWLARKAFFGRVPFPALHVDTQKKFPEMYAFRDHYSKEWGLDLKVDHCPPVEAIDPTLPPAARSAARKTEGLKLALNKYGFDGLIAGIRRDEEATRAKERVFSPRGLEGGWDVRDQPPEFWDQFNASPPPGAHLRIHPILHWTEADIWAYTKRENIPIIPLYLAKDGKRYRSLGDADITFPVPSTASTIDEILIELEGTKIPERAGRALDHETEDAFERLRVAGYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNZ6</accession>
    <name>Q3SNZ6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Secretion protein HlyD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_2742</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2742"/>
    <dbReference type="EnsemblBacteria" id="ABA05995">
        <property type="protein sequence ID" value="ABA05995"/>
        <property type="gene ID" value="Nwi_2742"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2742"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018816_2_1_5"/>
    <dbReference type="OMA" id="NELFPGM"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043602">
        <property type="entry name" value="CusB_dom_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032317">
        <property type="entry name" value="HlyD_D23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006143">
        <property type="entry name" value="RND_pump_MFP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00529">
        <property type="entry name" value="CusB_dom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16576">
        <property type="entry name" value="HlyD_D23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01730">
        <property type="entry name" value="RND_mfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229187" evidence="3">
        <location>
            <begin position="24"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="CusB_dom_1" evidence="4">
        <location>
            <begin position="50"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="HlyD_D23" evidence="5">
        <location>
            <begin position="66"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="107"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="401" mass="42957" checksum="C5304534EFACA1E7" modified="2005-10-11" version="1">MPRFNIRLAFLAIVIAGSSPLLGGCDEPTTADASAGTVIPEVSVITVVPKPHAIVRELPGRISPLRVAEVRPRVSGIITKRLFQQGSEVKAGDPLYQIDPEPFAVEVQAAEAALAKANAVLEQASHHARRIEKLTARHATSEAENEKAIANMRQAAADVAARKADLARARLHLDYTTIRAPIDGVVGAAQVTEGALVVQNDAASLATVQQLDTIYADFTQSVTELNKLRHAFESGDLERIAPGAAKVRLLLNDDKLYPLPGKFLFSEAKVDSSTGQVTLRGEFANPKRELLPGMYIRVLLEQGVDSDAIAVPQQAVQRDSSGGSEVFVVRDDDRVIVRPIRTDAVQHGYSMVSDGLNAGDRVVVEGFQKFTAGDKVRVVTWEADASPKTGAVPGETASARQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP09</accession>
    <name>Y2729_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable transcriptional regulatory protein Nwi_2729</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">Nwi_2729</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TACO1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315928.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SP09"/>
    <dbReference type="SMR" id="Q3SP09"/>
    <dbReference type="STRING" id="323098.Nwi_2729"/>
    <dbReference type="PRIDE" id="Q3SP09"/>
    <dbReference type="EnsemblBacteria" id="ABA05982">
        <property type="protein sequence ID" value="ABA05982"/>
        <property type="gene ID" value="Nwi_2729"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2729"/>
    <dbReference type="eggNOG" id="COG0217">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062974_2_2_5"/>
    <dbReference type="OMA" id="ETIMYEG"/>
    <dbReference type="OrthoDB" id="1304328at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.980">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00693">
        <property type="entry name" value="Transcrip_reg_TACO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017856">
        <property type="entry name" value="Integrase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002876">
        <property type="entry name" value="Transcrip_reg_TACO1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026564">
        <property type="entry name" value="Transcrip_reg_TACO1-like_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029072">
        <property type="entry name" value="YebC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12532">
        <property type="entry name" value="PTHR12532"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01709">
        <property type="entry name" value="Transcrip_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75625">
        <property type="entry name" value="SSF75625"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01033">
        <property type="entry name" value="TIGR01033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000257089" description="Probable transcriptional regulatory protein Nwi_2729">
        <location>
            <begin position="1"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="248" mass="27001" checksum="BC4EF68F8C97B447" modified="2005-10-11" version="1">MAGHSQFKNIMHRKGRQDAQKSKLFSKLAREITVAAKLGTPDPAMNPRLRAAVIAARAENMPKDNIERAIKKASGNDAESYDELRYEGYGPGGVAVIMEVLTDNRNRAASDIRSYFTKSGGNLGETGSVSFMFDRLGVIEYDADKASVDDMLEAAIEAGADDVVSDEGGHEIYASQDTFRDVAKALESTFGEARKVALIWKPQNTIAVDDNTGEKLLKLIDLLNEHDDVQNVYANFEVSDALIAKLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP31</accession>
    <name>Q3SP31_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2707</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315906.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05960">
        <property type="protein sequence ID" value="ABA05960"/>
        <property type="gene ID" value="Nwi_2707"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2707"/>
    <dbReference type="eggNOG" id="ENOG5033ZF5">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115294_0_0_5"/>
    <dbReference type="OMA" id="FAFTCFD"/>
    <dbReference type="OrthoDB" id="1886753at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229078" evidence="2">
        <location>
            <begin position="22"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="198" mass="21020" checksum="CE150AFD9CC4F777" modified="2005-10-11" version="1">MLARFVSGVLFAGAIAAPAMAGHLMNASEAKRFVAGKVFAFTCFDGTRGAGRVFEDGGAAGAVQFSGEGPVRHMRLPGNTLQVRGQTVCASIKGLPFEPCFNLDKKSERSFRGSVSGMSFAYCDFNHQGGRQMLLARAAARPYARSRVARTADASASNTEVTARVPTPRVESARLEPVKPDARPARAEGALQLRPSTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP58</accession>
    <name>Q3SP58_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC-type nitrate/sulfonate/bicarbonate transport systems periplasmic components</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2680</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315879.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2680"/>
    <dbReference type="EnsemblBacteria" id="ABA05933">
        <property type="protein sequence ID" value="ABA05933"/>
        <property type="gene ID" value="Nwi_2680"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2680"/>
    <dbReference type="eggNOG" id="COG0715">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028871_1_1_5"/>
    <dbReference type="OMA" id="DWKFEGP"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027939">
        <property type="entry name" value="NMT1/THI5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015168">
        <property type="entry name" value="SsuA/THI5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31528">
        <property type="entry name" value="PTHR31528"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09084">
        <property type="entry name" value="NMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229077" evidence="1">
        <location>
            <begin position="24"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="NMT1" evidence="2">
        <location>
            <begin position="52"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="353" mass="37584" checksum="40A322AD8006A834" modified="2005-10-11" version="1">MQVRSLRRLWCILLVLVSAVGVACPGVADNGADAGAPTQIRFSLDRPIDAAVAPVVLASTRGLFRAEGLSVTTAAAADTADAIARVAAGKSDLALADINALIRFRDQRGAPPVKAVFVLFDKAAYAFIARKSRGINALADIEGKTVGVIEGDPSIRLWPALARRNGLRLKTVKLQQISPAVREPMLSAGQLDAISGLSYLSAIDLRDRGIPADDIAVLRFADYGCAAYGKALIVNPAFAAGKPEAVKAFLRAVIAGLRLTIREPAKAVDDVLAQMDGGSRAVELERLHTVLSDNILTSEVRHSGIGGIDPARFETLLDQIAEDFRFRNRPAATDIFDSSFLPPASDRRITQVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPD7</accession>
    <name>Q3SPD7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Molybdenum cofactor biosynthesis protein B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2601</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">May be involved in the biosynthesis of molybdopterin.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 5">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the MoaB/Mog family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315804.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2601"/>
    <dbReference type="EnsemblBacteria" id="ABA05854">
        <property type="protein sequence ID" value="ABA05854"/>
        <property type="gene ID" value="Nwi_2601"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2601"/>
    <dbReference type="eggNOG" id="COG0521">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077358_2_2_5"/>
    <dbReference type="OMA" id="TGWDGIL"/>
    <dbReference type="OrthoDB" id="1528139at2"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00886">
        <property type="entry name" value="MogA_MoaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012245">
        <property type="entry name" value="MoaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013484">
        <property type="entry name" value="MoaB_proteobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008284">
        <property type="entry name" value="MoCF_biosynth_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43232">
        <property type="entry name" value="PTHR43232"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006443">
        <property type="entry name" value="MoaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="SSF53218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02667">
        <property type="entry name" value="moaB_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01078">
        <property type="entry name" value="MOCF_BIOSYNTHESIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoCF_biosynth" evidence="6">
        <location>
            <begin position="16"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR006443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="187" mass="20072" checksum="95BA10330FF19CEA" modified="2005-10-11" version="1">MPAIDETKPFVPLNIGVLTVSDTRSLEEDKSGAALAERLTAAGHKLAARAIVTDDVEAIRTLIKKWIADPGIDVIITTGGTGFTGRDVTPEAVEPLFEKRMDGFSIAFHMLSHAKIGTSAIQSRATAGTAGSTFVFCLPGSPGACRDGWDGILSHQLDYRTRPCNFVEIMPRLDEHLRRPKARGASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPM2</accession>
    <name>Q3SPM2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ornithine aminotransferase</fullName>
            <ecNumber evidence="3">2.6.1.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Ornithine--oxo-acid aminotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2516</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-ornithine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.13" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2516"/>
    <dbReference type="EnsemblBacteria" id="ABA05769">
        <property type="protein sequence ID" value="ABA05769"/>
        <property type="gene ID" value="Nwi_2516"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2516"/>
    <dbReference type="eggNOG" id="COG4992">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_10_3_5"/>
    <dbReference type="OMA" id="GHYWSGI"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00358"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050155">
        <property type="term" value="F:ornithine(lysine) transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004587">
        <property type="term" value="F:ornithine-oxo-acid transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010164">
        <property type="entry name" value="Orn_aminotrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986:SF18">
        <property type="entry name" value="PTHR11986:SF18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01885">
        <property type="entry name" value="Orn_aminotrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4 6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="422" mass="45052" checksum="FC78009647F4CCF0" modified="2005-10-11" version="1">MLYDHGPETHANRGAGDMDGSVQLEMRYGASNYAPLPVTIVRGKGVYLWDDGGRRYVDMMGAYSAASFGHSHPRLVQALTDQARQLDTVSRAYFNDRLGVFLARACRLTGMDLALPMNSGAEAVETALKAARKWAYKVKGVPDGRAEIIVCDGNFHGRTIAIVGFSSVAQYRDGFGPFPPGFRSVPFGDAAALRAAITPNTAAFLVEPIQGEGGIIVPPSGYLAEVAHICREHDVLLICDEIQSGLGRTGKLLACQHDGVTPDAVTLGKALGGGLLPVSLFLARREVMRVFSPGDHGSTFGGNPIAAAVGLAALDTLIEEGLVERSARLGAHLLARLRAIENPVIREVRGCGLFAGVELDRDSASAATVATQLLRAGVLTKDTHRNTIRFAPPLIIEETQIDWVTDRVAEVLNELARADAHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPQ8</accession>
    <name>Q3SPQ8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UPF0114 protein Nwi_2480</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2480</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the UPF0114 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315686.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2480"/>
    <dbReference type="EnsemblBacteria" id="ABA05733">
        <property type="protein sequence ID" value="ABA05733"/>
        <property type="gene ID" value="Nwi_2480"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2480"/>
    <dbReference type="eggNOG" id="COG2862">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097887_0_0_5"/>
    <dbReference type="OMA" id="HTIMWQV"/>
    <dbReference type="OrthoDB" id="1466526at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00143">
        <property type="entry name" value="UPF0114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005134">
        <property type="entry name" value="UPF0114"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020761">
        <property type="entry name" value="UPF0114_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38596">
        <property type="entry name" value="PTHR38596"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03350">
        <property type="entry name" value="UPF0114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00645">
        <property type="entry name" value="HI0507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="114"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="196" mass="21547" checksum="B66233DF9CC18251" modified="2005-10-11" version="1">MSPIPETPPSSKVAATTLRPLPMLIFSSRWLQLPLYIGLIVAQGVYVVLFLKELWHLIGHSFDFSEQQIMLVVLGLIDVVMISNLLVMVIVGGYETFVSRLNLKGHPDEPEWLGHVNASVLKIKLAMAIIGISSIHLLRTFIEAGNLGTERAMNYTQTGVMWQTIIHVVFIFSAIGIALADRIATESNGSNGETGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPX8</accession>
    <name>Q3SPX8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">3-demethylubiquinone-9 3-O-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.64</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2409</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.64" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315623.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2409"/>
    <dbReference type="EnsemblBacteria" id="ABA05663">
        <property type="protein sequence ID" value="ABA05663"/>
        <property type="gene ID" value="Nwi_2409"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2409"/>
    <dbReference type="eggNOG" id="COG2265">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1271201_0_0_5"/>
    <dbReference type="OrthoDB" id="1271665at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008689">
        <property type="term" value="F:3-demethylubiquinone-9 3-O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="Methyltranfer_dom" evidence="1">
        <location>
            <begin position="62"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="217" mass="24245" checksum="1AF81A4ECDDE768F" modified="2005-10-11" version="1">MNQKSSLRKVPQFVSCVLTANRPHYDYGEGYFYQSLAELGVSGLRSTEARLKAYRLLDHIRDKTVLDIGCNAGFLSVSLAKAAQHVTGLDINPFLSALARTAAQALGRTNTEFLATGFEDAPLRGPYDVVMSLANHSTFDGQTRYDLQSYFDRCISLLKPDGLFIFESHTPSFEGEGITQVCEMISQRLNVKQRDICNYGTYLDRGRTFIIAEHSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPY3</accession>
    <name>Q3SPY3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2404</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2404"/>
    <dbReference type="EnsemblBacteria" id="ABA05658">
        <property type="protein sequence ID" value="ABA05658"/>
        <property type="gene ID" value="Nwi_2404"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2404"/>
    <dbReference type="eggNOG" id="ENOG50313C2">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_675841_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="407" mass="46872" checksum="883B6647C97A6B1D" modified="2005-10-11" version="1">MAAARARFGDDGCAEIILRCRSLRSLDPGLAIKMIGGMALAIEKAFDHLDPRLVLTFTMDRYVMDVMERVARKREIDFIEMTTSIIPDQVMFLRRGKIIPLKEPTSQAMEAATTTLCHADFAPTYVSDTKKFSLSRFWRIYAYFALRGAYFNALRFLRRDPLNIHYIDALKSLRHKPRLRDVAVLSFMNPAWEARMRDVPRDRRVFLGLQLFPEASMDYWLNSQDMLQHDNVIVRYCEVLGGAGYHIYVKDHPLQFGFRQRGLMQRLSKLPSVTLVPYDVPAGVLIGQCAVSITFTGTIGFQAAIAGLCSVVTEPYYADERHYLHVRSFREIDDVAERIAQWSPPPDLSAARREMIRHLVAASVEGDYFTWRKFDRSNQAHRDKVQPLVRSLNRYLPRFLKPKPPYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ03</accession>
    <name>Q3SQ03_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GDP-L-fucose synthase</fullName>
            <ecNumber evidence="2">1.1.1.271</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">fcl</name>
        <name evidence="4" type="ordered locus">Nwi_2384</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the two-step NADP-dependent conversion of GDP-4-dehydro-6-deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>GDP-beta-L-fucose + NADP(+) = GDP-4-dehydro-alpha-D-rhamnose + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:18885"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57964"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.271"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nucleotide-sugar biosynthesis; GDP-L-fucose biosynthesis via de novo pathway; GDP-L-fucose from GDP-alpha-D-mannose: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Fucose synthase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.271" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2384"/>
    <dbReference type="EnsemblBacteria" id="ABA05638">
        <property type="protein sequence ID" value="ABA05638"/>
        <property type="gene ID" value="Nwi_2384"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2384"/>
    <dbReference type="eggNOG" id="COG0451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_18_0_5"/>
    <dbReference type="OMA" id="IHCAGRV"/>
    <dbReference type="UniPathway" id="UPA00128">
        <property type="reaction ID" value="UER00191"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050577">
        <property type="term" value="F:GDP-L-fucose synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070401">
        <property type="term" value="F:NADP+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042351">
        <property type="term" value="P:'de novo' GDP-L-fucose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05239">
        <property type="entry name" value="GDP_FS_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00956">
        <property type="entry name" value="GDP_fucose_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028614">
        <property type="entry name" value="GDP_fucose/colitose_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="3">
        <location>
            <begin position="13"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="2">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="17"/>
            <end position="23"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="111"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="169"/>
            <end position="172"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="2">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="2">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="316" mass="34693" checksum="08687DBED8001184" modified="2005-10-11" version="1">MVKTPFELAGKSVFVAGHRGMVGSALVRRLAQEHVTLLTVDRREVDLRDQAAVNQWFAGHHPQVVFLAAAKVGGIIANDTLRGEFIYDNLIIAANVIHAAHVHGVQKLLFLGSSCIYPKLAEQPLREDSLLTGPLEPTNEPYAIAKIAGVKMVEAYRRQYDSDFINVMPTNLYGPGDNYHPEYSHVVAGLIRRFHEARVAGASEVTVWGSGKPRREFLCVDDLADACIHLLKTYSDSELVNIGTGEDITIADFAHLVASTVGYGGRINFDTSRPDGTPRKLLDVSRLARLGWRAKVPLPEGIKLAYEAFLRSRAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ59</accession>
    <name>Q3SQ59_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2328</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05582">
        <property type="protein sequence ID" value="ABA05582"/>
        <property type="gene ID" value="Nwi_2328"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2328"/>
    <dbReference type="HOGENOM" id="CLU_2437843_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229255" evidence="1">
        <location>
            <begin position="37"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="90" mass="9481" checksum="94E3D630A6C0F4B7" modified="2005-10-11" version="1">MRQKHKWQTWIGVVTAFAFALQAFLAAAVSTQMAVAAPVDSFAICHSIADPADSRHPPSGTAHGAHHPCIVCSFASAGGLAPSLVRRQMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQB5</accession>
    <name>Q3SQB5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Squalene/phytoene synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2272</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315493.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2272"/>
    <dbReference type="EnsemblBacteria" id="ABA05526">
        <property type="protein sequence ID" value="ABA05526"/>
        <property type="gene ID" value="Nwi_2272"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2272"/>
    <dbReference type="eggNOG" id="COG1562">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037269_0_1_5"/>
    <dbReference type="OMA" id="DYCRYSA"/>
    <dbReference type="OrthoDB" id="1449625at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004311">
        <property type="term" value="F:farnesyltranstransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00683">
        <property type="entry name" value="Trans_IPPS_HH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017827">
        <property type="entry name" value="HSQ_synthase_HpnC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002060">
        <property type="entry name" value="Squ/phyt_synthse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044843">
        <property type="entry name" value="Trans_IPPS_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033904">
        <property type="entry name" value="Trans_IPPS_HH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31480:SF17">
        <property type="entry name" value="PTHR31480:SF17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00494">
        <property type="entry name" value="SQS_PSY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01212">
        <property type="entry name" value="Phytoene_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="SSF48576"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03464">
        <property type="entry name" value="HpnC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="292" mass="32563" checksum="12E1DD3262FD2E80" modified="2005-10-11" version="1">MPAANQLRSGKTHRDENFPVASRLIHPRHRALILAFYNFVRTADDIADHAELGEKDKLAWLDLFESELLGQGDSQPEAVNLRKALAQRSMPPRHAIDVLKAFRMDVTKLRYETWDDVIDYCRYSAMPVGRFMLDVHGESMSTWTASDALCAGLQINNHLQDCAKDYRNLNRVYLPRDALAASGVSVEMLGEDKSSPALLQCLHALAGRTEVLLDEGRPLAGEIRDTRLGLEVGVIQMFADKIVALLKARDPLSETVHLKPLQLLGYSAAGITMEMLRRLLRRRPVSNPAAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQD9</accession>
    <name>Q3SQD9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RNA polymerase, sigma subunit, ECF family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2248</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315470.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2248"/>
    <dbReference type="EnsemblBacteria" id="ABA05502">
        <property type="protein sequence ID" value="ABA05502"/>
        <property type="gene ID" value="Nwi_2248"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2248"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_3_0_5"/>
    <dbReference type="OMA" id="LVDMQGY"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="2">
        <location>
            <begin position="27"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="3">
        <location>
            <begin position="123"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="189" mass="21526" checksum="6E068F05CB333E59" modified="2005-10-11" version="1">MSATQAASDEALIARIAQGDRLAMQVLYGRHHVKVFRFGLRLVRNEQIAEDLISEVFLDVWRQAGKFEGRSAVSTWLLAIMRFKALSALRRRKDAELDDETANEIEDQSDDPEVTVQKKDTGNALRKCLMQLSAEHREIVDLVYYHEKSVEEVAEIVGIPENTVKTRLFYARKKLAGLLQEAGIERGWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQG1</accession>
    <name>Q3SQG1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATPase involved in DNA replication initiation</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2226</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2226"/>
    <dbReference type="EnsemblBacteria" id="ABA05480">
        <property type="protein sequence ID" value="ABA05480"/>
        <property type="gene ID" value="Nwi_2226"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2226"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0593">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_841533_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06571">
        <property type="entry name" value="Bac_DnaA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1750.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013159">
        <property type="entry name" value="DnaA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045599">
        <property type="entry name" value="DUF6456"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010921">
        <property type="entry name" value="Trp_repressor/repl_initiator"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08299">
        <property type="entry name" value="Bac_DnaA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20057">
        <property type="entry name" value="DUF6456"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00760">
        <property type="entry name" value="Bac_DnaA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48295">
        <property type="entry name" value="SSF48295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_DnaA_C" evidence="2">
        <location>
            <begin position="23"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="330" mass="36052" checksum="0DF485970A45FAAD" modified="2005-10-11" version="1">MLLIHPSTSPASVRPSPTSATRICHAIARLVAEEFELDAAAVIAPTRGAPRAAYARQVAMYLAHVGFALSFEAIGRAFDRDRTTVSHACRVVEDSRDDAGLDRRLAALEAMCAVVMTASKERPMPASDRGSRQSSRRSKSRPAKIVDRFRARHLALAEREIMTDAGVATVMVDDSESPLAWLARRKGRDGRAMISRDQFAAGERLRADFTRGNLTPRVTSNWSAPTGGRPRGSGAGPCEMTDLIVAARQRVRLALEACGPEFSGLLMDVCCFLRGLEDVERERGWPSRSAKVVLQLALDRLARHYGIQARAETSAHVRTWLASDARFTSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQI9</accession>
    <name>Q3SQI9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2198</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2198"/>
    <dbReference type="EnsemblBacteria" id="ABA05452">
        <property type="protein sequence ID" value="ABA05452"/>
        <property type="gene ID" value="Nwi_2198"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2198"/>
    <dbReference type="eggNOG" id="COG3637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080719_0_0_5"/>
    <dbReference type="OMA" id="DAFWLGP"/>
    <dbReference type="OrthoDB" id="1710733at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031485">
        <property type="entry name" value="CBP_BcsS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17036">
        <property type="entry name" value="CBP_BcsS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229059" evidence="1">
        <location>
            <begin position="22"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="248" mass="27358" checksum="8E190D4C1E3D04BC" modified="2005-10-11" version="1">MSVRAVCMAALLAWPMISRSAAQPVTGPPLTDNLIGGSKPERFLFFSGVDVWRNGLAGYDGMLLAPQGDLNKDGFLVRLFMSDNIEFYDTATRRFTTNIFRASALPGWRIKRGDLEVRVFAGPTIEIRGFKPAVQIDKLSGSRFGVQLASELWWEPTPAMMVAAAAAVTTINSVYSARAAAGWRLFDRFWIGPEVSMSTDRFSTQYRLGAHVTGFRTGVLEWSAGVGLVQDSFDRNGVYGRISALFRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQM4</accession>
    <name>Q3SQM4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2163</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315386.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2163"/>
    <dbReference type="EnsemblBacteria" id="ABA05417">
        <property type="protein sequence ID" value="ABA05417"/>
        <property type="gene ID" value="Nwi_2163"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2163"/>
    <dbReference type="eggNOG" id="ENOG5030YET">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1634084_0_0_5"/>
    <dbReference type="OMA" id="RADRCDD"/>
    <dbReference type="OrthoDB" id="1582513at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229133" evidence="1">
        <location>
            <begin position="28"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="156" mass="16897" checksum="25DE0B9EFA2D6682" modified="2005-10-11" version="1">MTSPRRCLTASLCVAAAAMLASPPALADRCDDLAAQLKSQIDGLNIGKTAANVIYLSHPATTQLILGCSNRKFTNELIAGTDTRKPNPEFYDLVARASAIIFTIPIADTLRGATRCMKRMGLLRGDDVQTRFRRLNMHCTRTKTSSAIAVSRGIDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQR7</accession>
    <name>Q3SQR7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transposase IS3/IS911</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1573</name>
        <name evidence="4" type="ordered locus">Nwi_2119</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="4">Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1573"/>
    <dbReference type="EnsemblBacteria" id="ABA04834">
        <property type="protein sequence ID" value="ABA04834"/>
        <property type="gene ID" value="Nwi_1573"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05374">
        <property type="protein sequence ID" value="ABA05374"/>
        <property type="gene ID" value="Nwi_2119"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1573"/>
    <dbReference type="KEGG" id="nwi:Nwi_2119"/>
    <dbReference type="eggNOG" id="COG2963">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027402_33_6_5"/>
    <dbReference type="OMA" id="WVKRVEV"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002514">
        <property type="entry name" value="Transposase_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01527">
        <property type="entry name" value="HTH_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0814">Transposable element</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="67"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="109" mass="12508" checksum="92EC107E47A279D5" modified="2005-10-11" version="1">MMSKTRNRFSPEVRARAVRMVFDHEKEHRSRWAAVSSIATKIGCTAQSLNEWVKKAEVDSGTRAGLPSEVTEKLKALERENRELRQANEILRKASAYFAMAELDRRSKT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQS3</accession>
    <name>CH601_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chaperonin GroEL 1</fullName>
            <ecNumber evidence="1">5.6.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">60 kDa chaperonin 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Chaperonin-60 1</fullName>
            <shortName evidence="1">Cpn60 1</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">groEL1</name>
        <name evidence="1" type="synonym">groL1</name>
        <name type="ordered locus">Nwi_2113</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Together with its co-chaperonin GroES, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + a folded polypeptide = ADP + phosphate + an unfolded polypeptide.</text>
            <dbReference type="EC" id="5.6.1.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a cylinder of 14 subunits composed of two heptameric rings stacked back-to-back. Interacts with the co-chaperonin GroES.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the chaperonin (HSP60) family.</text>
    </comment>
    <dbReference type="EC" id="5.6.1.7" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315341.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SQS3"/>
    <dbReference type="SMR" id="Q3SQS3"/>
    <dbReference type="STRING" id="323098.Nwi_2113"/>
    <dbReference type="EnsemblBacteria" id="ABA05368">
        <property type="protein sequence ID" value="ABA05368"/>
        <property type="gene ID" value="Nwi_2113"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2113"/>
    <dbReference type="eggNOG" id="COG0459">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016503_3_0_5"/>
    <dbReference type="OMA" id="LKDQHMN"/>
    <dbReference type="OrthoDB" id="265347at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03344">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00600">
        <property type="entry name" value="CH60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018370">
        <property type="entry name" value="Chaperonin_Cpn60_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001844">
        <property type="entry name" value="Cpn60/GroEL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00298">
        <property type="entry name" value="CHAPERONIN60"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="SSF48592"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="SSF52029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="SSF54849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02348">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00296">
        <property type="entry name" value="CHAPERONINS_CPN60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000256936" description="Chaperonin GroEL 1">
        <location>
            <begin position="1"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="30"/>
            <end position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="87"/>
            <end position="91"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="415"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="480"/>
            <end position="482"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="496"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00600"/>
        </source>
    </evidence>
    <sequence length="542" mass="57694" checksum="FA08A075B413F495" modified="2005-10-11" version="1">MAAKEVRFSSDAREKMLRGVDTLANAVKVTLGPKGRNVVIEKSFGAPRITKDGVTVAKEIELDDKFENMGAQMVREVASKTNDLAGDGTTTATVLAQAIVKEGAKAVAAGMNPMDLKRGIDLAVEAVVKDLTKNAKKITSNSEIAQVATISANGDTEIGRFLAEAMQKVGNEGVITVEEAKSLETELEVVEGMQFDRGYVSPYFVTDAEKMRVEFEDPYVLIHEKKLSGLQAMVPLLESVVQSGKPLLVIAEDVEGEALATLVVNKLRGGLKVAAVKAPGFGDRRKAMLEDIAILTGGTAISEDLGIKLENVTLNMLGRAKKVVIDKENTTIVDGAGRKKDIEARVTQIKAQIEETTSDYDREKLQERLAKLAGGVAVIRVGGATEVEVKERKDRVDDAMHATRAAVEEGILPGGGVALLRALKALDAVKPDNPDQKAGVDIVRRAIQVPARQIIQNAGEDGSLVVGKLLEKNTYNWGFNAATGEYQDLVGVGVIDPAKVVRTALQDAASVASLLITTEALVAEKPKKDVAPALPPGGGMDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQU0</accession>
    <name>Q3SQU0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">TrbL/VirB6 plasmid conjugal transfer protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_2094</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TrbL/VirB6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2094"/>
    <dbReference type="EnsemblBacteria" id="ABA05351">
        <property type="protein sequence ID" value="ABA05351"/>
        <property type="gene ID" value="Nwi_2094"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2094"/>
    <dbReference type="eggNOG" id="COG3846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049867_0_0_5"/>
    <dbReference type="OMA" id="WATRMKR"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030255">
        <property type="term" value="P:protein secretion by the type IV secretion system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014150">
        <property type="entry name" value="Conjugal_tfr_TrbL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007688">
        <property type="entry name" value="Conjugal_tfr_TrbL/VirB6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04610">
        <property type="entry name" value="TrbL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02783">
        <property type="entry name" value="TrbL_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="138"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="236"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="277"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="376"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="376"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA05351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="414" mass="40782" checksum="DCD742ACE691BD39" modified="2005-10-11" version="1">MNTGVIDRFLETFTRYIDSGFGLLGGEIGFLTSTLIVIDITLAGLFWAWGADEDVIARLIKKTLYIGVFAFIIGNFNKLAGIVFQSFSGLGLKAGGSSLTAETLLQPGRVAQVGIDAGNPILKAAGDLMGYISFFENFVQIMVLMIAWAIVLIAFFILAVQIFVTLIEFKLTTLAGFVLIPFALFNKTAFLAEKVLGNIVASGVKILVLSVIVGIGSGLFSEFTSGFSGTPTITDALALVLGALSLMGLSIFGPGIATGLVSGAPQLGAGAAVGTGLAAAGIGAAGVMGAGAALGGGAAAIGAAGHMTAAAARGGAHLAGATSGAYSGGASGHAGMGAVAGGLGGVAKAGASAAIQPVRQAASRAAASLKSTYAAGRAATTGGQTSPTGAGASASQSPSASGAPPAWATRMKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQV1</accession>
    <name>Q3SQV1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2083</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2083"/>
    <dbReference type="EnsemblBacteria" id="ABA05340">
        <property type="protein sequence ID" value="ABA05340"/>
        <property type="gene ID" value="Nwi_2083"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2083"/>
    <dbReference type="eggNOG" id="ENOG5033S53">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1538488_0_0_5"/>
    <dbReference type="OMA" id="ALASEWY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="174" mass="19628" checksum="44BBCCE707DB6DC9" modified="2005-10-11" version="1">MYLIALDRNPAFVSKMKDDDYKSRLARASLHLNAPDLNVTADVRRMLEDFVAQGSQGAKAIQVATLLEGSQFDRLYRTYRDISGDAAHVSVTSLNRHYIENPKDQDAILVIHPALDDLDLHMTMTELGISMTIATLILMKIKEKTDLWDEFQSLLHRYRELSKTNQEGAGAHVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR32</accession>
    <name>Q3SR32_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2000</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2000"/>
    <dbReference type="EnsemblBacteria" id="ABA05259">
        <property type="protein sequence ID" value="ABA05259"/>
        <property type="gene ID" value="Nwi_2000"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2000"/>
    <dbReference type="eggNOG" id="ENOG5032YM4">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165468_0_0_5"/>
    <dbReference type="OMA" id="HAYLHRK"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="100" mass="10946" checksum="B4B345192C4D7CD6" modified="2005-10-11" version="1">MTPDAFKASLANAEPPQGVSVPLAALWWSAKGDWNKAHVIVQPVVGPEAAWVHAYLHRLDGREIGNAAYWYRRADKPFGKGSSEDEWSQIVAELLGGYNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRI6</accession>
    <name>Q3SRI6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 8">Citrate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_1845</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16845"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 6 9">Belongs to the citrate synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315101.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1845"/>
    <dbReference type="EnsemblBacteria" id="ABA05105">
        <property type="protein sequence ID" value="ABA05105"/>
        <property type="gene ID" value="Nwi_1845"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1845"/>
    <dbReference type="eggNOG" id="COG0372">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025068_0_0_5"/>
    <dbReference type="OMA" id="TVGWCAQ"/>
    <dbReference type="OrthoDB" id="1412360at2"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00717"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004108">
        <property type="term" value="F:citrate (Si)-synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06114">
        <property type="entry name" value="EcCS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016142">
        <property type="entry name" value="Citrate_synth-like_lrg_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019810">
        <property type="entry name" value="Citrate_synthase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024176">
        <property type="entry name" value="Citrate_synthase_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010953">
        <property type="entry name" value="Citrate_synthase_typ-I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001369">
        <property type="entry name" value="Citrate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00143">
        <property type="entry name" value="CITRTSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="SSF48256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01798">
        <property type="entry name" value="cit_synth_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00480">
        <property type="entry name" value="CITRATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="active site" evidence="7">
        <location>
            <position position="309"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001369-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA05105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="433" mass="48581" checksum="A92721C1844431DA" modified="2005-10-11" version="1">MNATSTKNATLTVDDKSYEFPVLSGTVGPDVVDIGKLYAQTGMFTYDPAFTSTASCQSKITYIDGDEGVLEYRGYPIEQLAEKGDFIETCYLLLYGELPTKSQKHDFEERVTHHTMVHEQMTRFFQSFRRDAHPMAVMVAAVGALAAFYHDSTDINDPKQRMIASMRMVAKMPTLAAMAYKYTIGQPFIYPKNSLNFAENFLHMCFAVPCEDYDINPVLADALDKIFILHADHEQNASTSTVRIAGSSGANPFACIAAGIACLWGPAHGGANEAALNMLYEIGTVDRIPEFIDKVKDKNSDVRLMGFGHRVYKNYDPRAKIMQNMCHAVLNETGHGNDPMLKVALELEKIALNDEYFINRKLYPNVDFYSGLTLKAMGFPTSMFTVLFAVARTVGWISQWSEMIEDPQQKIGRPRQLYTGKTRRDYVDMAQRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRL7</accession>
    <name>Q3SRL7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Dihydrolipoyl dehydrogenase</fullName>
            <ecNumber evidence="3 13">1.8.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">Nwi_1813</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>(R)-N(6)-dihydrolipoyl-L-lysyl-[protein] + NAD(+) = (R)-N(6)-lipoyl-L-lysyl-[protein] + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:15045"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="EC" id="1.8.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 13">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="11 13">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="13">The active site is a redox-active disulfide bond.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.4" evidence="3 13"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315070.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1813"/>
    <dbReference type="EnsemblBacteria" id="ABA05074">
        <property type="protein sequence ID" value="ABA05074"/>
        <property type="gene ID" value="Nwi_1813"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1813"/>
    <dbReference type="eggNOG" id="COG1249">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016755_0_2_5"/>
    <dbReference type="OMA" id="WASMLND"/>
    <dbReference type="OrthoDB" id="267896at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004148">
        <property type="term" value="F:dihydrolipoyl dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006258">
        <property type="entry name" value="Lipoamide_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="SSF55424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01350">
        <property type="entry name" value="lipoamide_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11 13" id="KW-0274">FAD</keyword>
    <keyword evidence="4 13" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7 11 13" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 13 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9 13" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="15">
        <location>
            <begin position="6"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="Pyr_redox_dim" evidence="14">
        <location>
            <begin position="358"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="456"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="192"/>
            <end position="199"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="329"/>
            <end position="332"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="12">
        <location>
            <begin position="43"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ABA05074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="477" mass="51036" checksum="37203DEFA48822E1" modified="2005-10-11" version="1">MADTHFDVIIIGSGPGGYVAAIRAAQLGLKTAIVEKQHLGGICLNWGCIPTKALLRSAEIYHYMRHAKDYGLSAEKVSYDPTAVVQRSRGVSKRLNEGVGFLMKKNKITVIWGEASVDAPGKITVKTSDPRIKTEPPKGVLGEGSYQAKHIVVATGARPRVLPGLEQDGKLVWTYFEAMVAPKIPKSLLVVGSGAIGIEFASFFRTMGAEVTVVEVLPQILPVEDAEIAALARKQFEKQGIRIMTSTKVTKLDRKADSVVATIDAGDGKPVAAEFERVISAVGVVGNIENLGLEKLGVKTDRGCIVIDGYGRTNVPGIYAIGDVAGPPMLAHKAEHEGVICIEAIKDLKPHPMDRSLIPGCTYCHPQIASVGLTEAKAKAAGKDIRVGRFPFAGNGKAIALGEDQGLVKVIFDSKTGQLLGAHMIGAEVTELIQGYVVAMNLETTEQELMHTIFPHPTLSEMMKEAVLDAYGRALNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRM0</accession>
    <name>Q3SRM0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Exodeoxyribonuclease III</fullName>
            <ecNumber evidence="4">3.1.11.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1810</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.2" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315067.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1810"/>
    <dbReference type="EnsemblBacteria" id="ABA05071">
        <property type="protein sequence ID" value="ABA05071"/>
        <property type="gene ID" value="Nwi_1810"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1810"/>
    <dbReference type="eggNOG" id="COG0708">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027539_0_1_5"/>
    <dbReference type="OMA" id="YEVAHVG"/>
    <dbReference type="OrthoDB" id="1893707at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008853">
        <property type="term" value="F:exodeoxyribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09086">
        <property type="entry name" value="ExoIII-like_AP-endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020847">
        <property type="entry name" value="AP_endonuclease_F1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020848">
        <property type="entry name" value="AP_endonuclease_F1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037493">
        <property type="entry name" value="ExoIII-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43250">
        <property type="entry name" value="PTHR43250"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="SSF56219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00633">
        <property type="entry name" value="xth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00726">
        <property type="entry name" value="AP_NUCLEASE_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00728">
        <property type="entry name" value="AP_NUCLEASE_F1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51435">
        <property type="entry name" value="AP_NUCLEASE_F1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endo/exonuclease/phosphatase" evidence="3">
        <location>
            <begin position="4"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="266" mass="30022" checksum="87F088702082A6AA" modified="2005-10-11" version="1">MRIATWNVNSVRQRLDHLITWLRETAPDVVCLQEIKCVDEQFPREAIEALGYNVVTHGQKTFNGVALLSKFPFDETRPRLAGDDGDVQARFLEGVVTLEHGAVRVACLYLPNGNPPNTEKYPYKLKWISRLIEYSRERLKSEEAFILAGDFNVIPAPCDVYNSAAWVDDALFRPRTRQAFQTLLGLGLTDALRAVSDAAGEYTFWDYQAGAWQKNNGLRIDHLLLSPQASDRLSGAGIDRHVRGWEKASDHVPVWIDLDLETAGRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRM1</accession>
    <name>Q3SRM1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">HesB/YadR/YfhF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1809</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HesB/IscA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315066.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1809"/>
    <dbReference type="EnsemblBacteria" id="ABA05070">
        <property type="protein sequence ID" value="ABA05070"/>
        <property type="gene ID" value="Nwi_1809"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1809"/>
    <dbReference type="eggNOG" id="COG0316">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069054_5_3_5"/>
    <dbReference type="OMA" id="SLDYCTG"/>
    <dbReference type="OrthoDB" id="1891602at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.300.12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000361">
        <property type="entry name" value="FeS_biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016092">
        <property type="entry name" value="FeS_cluster_insertion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017870">
        <property type="entry name" value="FeS_cluster_insertion_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035903">
        <property type="entry name" value="HesB-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01521">
        <property type="entry name" value="Fe-S_biosyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89360">
        <property type="entry name" value="SSF89360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00049">
        <property type="entry name" value="TIGR00049"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01152">
        <property type="entry name" value="HESB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe-S_biosyn" evidence="2">
        <location>
            <begin position="5"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="110" mass="11634" checksum="0DC657002CD24CEF" modified="2005-10-11" version="1">MTEANITVSERAARRIREILKSEGDGAMLRISVEGGGCSGFQYKFDIERAKADDDLVIARDSAVVLVDPASVPFLSGSEVDFVDDLIGASFRVVNPNATASCGCGTSFSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRR7</accession>
    <name>Q3SRR7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1763</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315020.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1763"/>
    <dbReference type="EnsemblBacteria" id="ABA05024">
        <property type="protein sequence ID" value="ABA05024"/>
        <property type="gene ID" value="Nwi_1763"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1763"/>
    <dbReference type="eggNOG" id="COG5473">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067791_0_0_5"/>
    <dbReference type="OMA" id="VIPILGH"/>
    <dbReference type="OrthoDB" id="1781385at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018692">
        <property type="entry name" value="DUF2189"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09955">
        <property type="entry name" value="DUF2189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="309" mass="33509" checksum="D868CBD37D8B66B0" modified="2005-10-11" version="1">MATSYPVDVTSHSGMAGADRPVVRHIGLSDLFDALRLGWEDFKAVPSHAIVLCVIYPVIGIVLARIALGYSVLPILFPLAAGFALIGPFAALGLYELSYRRELGKEASAWHAFSVLRAPSFGSMLGLGGLLLVIFFIWLATARAIYVSTFGNTPAAVIPDFFTQVFTTTKGLQMLMMGVGVGFLFAAVALCISIVAFPLMLHQHADMTDAILTSLRVVAKNPIAAAEWGLIVAVLLTLGSIPFFLGLTVVMPLLGHATWHLYRKAIESPLTSSAHEPRETEKEKRYAADFPSVLFPPYGRKHRFFDSAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRS5</accession>
    <name>Q3SRS5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Response regulator receiver</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1755</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315012.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1755"/>
    <dbReference type="EnsemblBacteria" id="ABA05016">
        <property type="protein sequence ID" value="ABA05016"/>
        <property type="gene ID" value="Nwi_1755"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1755"/>
    <dbReference type="eggNOG" id="COG0784">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_17_5"/>
    <dbReference type="OMA" id="NWQYHTS"/>
    <dbReference type="OrthoDB" id="1630092at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="6"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="146" mass="16330" checksum="A54AB1DE36952FB4" modified="2005-10-11" version="1">MSTPVTIIMIEDDEGHARLIERNIRRSGVNNEIKSFTTGTEAVNYLFGADGNGLPHKGESLLILLDLNLPDMTGIEILRRVKENRYLKCAPVVVLTTTDDSQEIKRCYELGCSVYITKPVNYDNFANAIRQLGLFFSIIQVPQATP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRT3</accession>
    <name>Q3SRT3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">K(+)-insensitive pyrophosphate-energized proton pump</fullName>
            <ecNumber evidence="9">7.1.3.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Membrane-bound proton-translocating pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Pyrophosphate-energized inorganic pyrophosphatase</fullName>
            <shortName evidence="9">H(+)-PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">hppA</name>
        <name evidence="10" type="ordered locus">Nwi_1747</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Proton pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for proton movement across the membrane. Generates a proton motive force.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>diphosphate + H(+)(in) + H2O = 2 H(+)(out) + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:13973"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="EC" id="7.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell inner membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. K(+)-insensitive subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="7.1.3.1" evidence="9"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315004.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1747"/>
    <dbReference type="EnsemblBacteria" id="ABA05008">
        <property type="protein sequence ID" value="ABA05008"/>
        <property type="gene ID" value="Nwi_1747"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1747"/>
    <dbReference type="eggNOG" id="COG3808">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008743_3_1_5"/>
    <dbReference type="OMA" id="MATTAMQ"/>
    <dbReference type="OrthoDB" id="98036at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009678">
        <property type="term" value="F:pyrophosphate hydrolysis-driven proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01129">
        <property type="entry name" value="PPase_energized_pump"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004131">
        <property type="entry name" value="PPase-energised_H-pump"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31998">
        <property type="entry name" value="PTHR31998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03030">
        <property type="entry name" value="H_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001265">
        <property type="entry name" value="H+-PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01104">
        <property type="entry name" value="V_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="57"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="119"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="235"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="262"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="293"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="328"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="374"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="405"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="469"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="516"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="588"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="616"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="site" description="Determinant of potassium independence" evidence="9">
        <location>
            <position position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA05008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="706" mass="71981" checksum="3AA9F551891444D1" modified="2005-10-11" version="1">MTALVGIVLCGALSIVYAGWATSSVLRSDAGNPRMQEIAAAVREGAQAYLRRQYTTIGMVGIVIFALLAYFLGVLVAVGFAIGAILSGAAGFIGMNVSVRANVRTAQAATKSLAGGLELAFKAGAITGMLVAGLALLGVTIYFAYLIHGAGYAANSRTVVDALVALGFGASLISIFARLGGGIFTKGADVGGDLVGKVEAGIPEDDPRNPATIADNVGDNVGDCAGMAADLFETYAVTAVATMVLAAIFFATSPLLVNMMTLPLAIGGICIITSIIGTFFVRLGNSQSIMGALYKGLIATGVLSLVGVGAVIYWLVGFGELAGVDYTGLDLFACGVAGLVVTGLIIWITEYYTGTDYRPVKSIAKASVTGHGTNVIQGLAISMEATALPAIVIIAGILVTYGLAGLFGIAIATTTMLALAGMIVALDAFGPVTDNAGGIAEMAGLPKEVRKSTDALDAVGNTTKAVTKGYAIGSAGLGALVLFAAYNQDLKFFIGDAAQHTYFQGVNPDFSLNNPYVVVGLLFGGLLPYLFGAMGMTAVGRAAGAIVEEVRRQFREKPGIMRGTDKPDYGKAVDLLTKAAIKEMIIPSLLPVLSPIVVYFLIYAIAGGGVAGKSAAFSAVGAMLLGVIVTGLFVAISMTSGGGAWDNAKKYIEDGHHGGKGSDAHKAAVTGDTVGDPYKDTAGPAVNPMIKITNIVALLLLAVLAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="130" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRT6</accession>
    <name>Q3SRT6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_1744</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315001.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1744"/>
    <dbReference type="EnsemblBacteria" id="ABA05005">
        <property type="protein sequence ID" value="ABA05005"/>
        <property type="gene ID" value="Nwi_1744"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1744"/>
    <dbReference type="eggNOG" id="COG2202">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_323071_0_0_5"/>
    <dbReference type="OMA" id="MNRYMAG"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004673">
        <property type="term" value="F:protein histidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="279"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="11">
        <location>
            <begin position="298"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="9">
        <location>
            <begin position="366"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="10">
        <location>
            <begin position="441"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="9">
        <location>
            <begin position="494"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="10">
        <location>
            <begin position="568"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="654"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="58"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA05005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="893" mass="98492" checksum="056B81B517A457C9" modified="2005-10-11" version="1">MPSGFGAFFQRMKSSSLSIGQITFGGFFLVLAIIVITSVANVVATRHLDNTFAELQRLDSVVDLAEELDRRMNELRLAARDYVTDPQAQSSRVWGATSSLNEMLKKTRIKLAPEQQEMIDGVTPRLEHYRKGIEQAANLIGDRAKLIETLPPLREKLELIIEQSADQSASRALFRAQSDVAAALLGHDPTTAERAAENMRAIRVDEPKVRAAVEAYADAVIAISLRERQIADLDRDVLGAEGRLIGQVTELLRELSAERERVLSRDFARTLAETKWQSIILGSVGIVIGLLAAVFVVRRTVRPLKAIARAIRKLAGGQKETSIPSTEVDNEIGDIARAAEVFRRTLAEADSAREAAVRALAEQRLAEESYRKLFEGSVDGIYVTTPAGQLLDANPALARMMGYDTPDDLFRGIGNSAQTVYVDTDARAKYQQRMERDGTVRGFEYQVRQRDGGILWLSDSATAVRDEDGRIVRYEGVVRDITDQRRAEETIAEGRRLLQLVIDTVPAVINVKNAEMRYVLMNRYMAGIFGIEPADAIGRTAGEVMSHDDAEKTDETDRRVLESGKELGFYEEEYQDSSGEPRQWLVNKLPIRDADGRVENIVTVALDIGDRKRSEQEMRRAKDAAEAALSNLRATQDSLIEAEKLAALGRLVAGVAHEVNNPVGISLTVASSLERKIALFAAEVERGQLRRSSLNDFIAANRDAASQLVANLNRAAELVQSFKQVAADRSYSDKRTFDLGELTEQVVMSLRPALRKHHVALTVDCEPDLTMNSYPGPYGQVLTNLFLNSVAHAFPDGKGGTVNITVRRVGGDNVEILFSDDGCGMNPDVRRKAFDPFFTTRRDQGGTGLGLHIVYNIVTSRLGGRIELDSDSGQGTRIHIVLPRVAPMELAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS72</accession>
    <name>Q3SS72_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1608</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314873.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1608"/>
    <dbReference type="EnsemblBacteria" id="ABA04869">
        <property type="protein sequence ID" value="ABA04869"/>
        <property type="gene ID" value="Nwi_1608"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1608"/>
    <dbReference type="eggNOG" id="COG1430">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097039_2_1_5"/>
    <dbReference type="OMA" id="TMWMKNT"/>
    <dbReference type="OrthoDB" id="1934378at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003795">
        <property type="entry name" value="DUF192"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038695">
        <property type="entry name" value="Saro_0823-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37953">
        <property type="entry name" value="PTHR37953"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02643">
        <property type="entry name" value="DUF192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="163" mass="17748" checksum="91326F9775F472F5" modified="2005-10-11" version="1">MLGLRDVGFGWFRAGLGIVLFSWIVVVAPDDGGVHAAEFQPLEIATRTGVKIFSVEMATTPEEKATGLMHRKHLPDGQGMLFDFSPAQQVSMWMKNTYISLDMIFIGSNGRIVRIAENTEPLSTRIISSRGLAKGVLEVIGGTAKKYGIATGDRVSHPLFNGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSD3</accession>
    <name>Q3SSD3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1547</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1547"/>
    <dbReference type="EnsemblBacteria" id="ABA04808">
        <property type="protein sequence ID" value="ABA04808"/>
        <property type="gene ID" value="Nwi_1547"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1547"/>
    <dbReference type="eggNOG" id="COG3747">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1676158_0_0_5"/>
    <dbReference type="OMA" id="GWMSAEG"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006448">
        <property type="entry name" value="Phage_term_ssu_P27"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05119">
        <property type="entry name" value="Terminase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="156" mass="18246" checksum="8C17A5D6F23FC626" modified="2005-10-11" version="1">MRGAKPKLRNVIPMRPDDVDAEKLRKKMMQRAIRRLMPRDLPAEVEKEYKRVAAILADPSVDRLKARYIDTIIEYCRCTVRMRGLRASMPTLAHEIYRVKTRNGDQVKSHPHVAQVNESWRQWRSLIAMLGLSPTDERNLLPGQGDLFDENDQYFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSF5</accession>
    <name>Q3SSF5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Pyridoxal-5'-phosphate-dependent enzyme, beta subunit</fullName>
            <ecNumber evidence="4">2.5.1.47</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1525</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="2.5.1.47" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1525"/>
    <dbReference type="EnsemblBacteria" id="ABA04786">
        <property type="protein sequence ID" value="ABA04786"/>
        <property type="gene ID" value="Nwi_1525"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1525"/>
    <dbReference type="eggNOG" id="COG0031">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021018_1_0_5"/>
    <dbReference type="OMA" id="CNGGFFA"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004124">
        <property type="term" value="F:cysteine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PALP" evidence="3">
        <location>
            <begin position="16"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="352" mass="37936" checksum="E662260149923EAA" modified="2005-10-11" version="1">MRILHLMRIDNDVVEAIGNTPLIKLKRASEATGCTILGKAEFMNPGQSVKDRAGKQMILEAEKRGDLKPGGLVVEGTAGNTGIGLAIVASARGYRTMIVIPETQSREKKDMLRLCGAELVEVPALPYSNPNNYQHIARRLADELRESEPNGVLFADQWNNLDNPKAHYVSTGPEIWEQTDGKVDGFICSIGTGGTLAGVSAFLREKNKDIVIGVADPRGAAMYELFKNGVAKPTAGGSITEGIGLGRVTPVIADARVDEAYLVPDEEAVPVIYDLLEHEGLCLGGSTGVNVAGAIRLARQLGPGHTVVTILCDSGNRYQSKLFNPDFMRSKNLPVPQWLERRSTVKVPLAEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSH3</accession>
    <name>Q3SSH3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase</fullName>
            <ecNumber evidence="6">1.14.13.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_1507</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UbiH/COQ6 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.13.-" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314774.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1507"/>
    <dbReference type="EnsemblBacteria" id="ABA04768">
        <property type="protein sequence ID" value="ABA04768"/>
        <property type="gene ID" value="Nwi_1507"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1507"/>
    <dbReference type="eggNOG" id="COG0654">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009665_8_1_5"/>
    <dbReference type="OMA" id="KRWFARQ"/>
    <dbReference type="OrthoDB" id="867226at2"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010971">
        <property type="entry name" value="UbiH/COQ6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01988">
        <property type="entry name" value="Ubi-OHases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_3" evidence="5">
        <location>
            <begin position="12"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="393"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="393"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="410"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA04768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="424" mass="45273" checksum="2F22E25636950564" modified="2005-10-11" version="1">MNESAKPVAIFDVAVVGGGPAGLAAAIALSHAGARTALIARRVGYGDNRTTALLGGSIDLLRELDVWPRCEADAAPLRVMRLVDDTGRLIRAPEVRFSAAEIGRDAFGYNIANRALMAAMEARAAEAENLTRIDGEAEQVTPGDTVVTITTRQGDELSARLAVGADGRQSLCRKAACIGITRRALDQAALTFNVAHSRPHKNTSTEFHTPHGPCVFVPLPGDRSSVVWVASPREVERLAALSDDELAEAAERRSHSILGRLEVGQGRNKFPLGIERPARFAGNRIVLVGESAHVLPPIGAQGLNLGLRDAADIAEIVGDVLSSGEDPGASPALARFETARRSDVLSRTFAIDVANRSLLSDFLPIQTARAFGLHLISKVGPLRRLAMREGLAPSWRRRPHREPRSKAFSGEVDTGSSQENASKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSH4</accession>
    <name>Q3SSH4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 17">Phosphatidylcholine synthase</fullName>
            <shortName evidence="17">PC synthase</shortName>
            <shortName evidence="17">PCS</shortName>
            <ecNumber evidence="6 17">2.7.8.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16 17">CDP-diglyceride-choline O-phosphatidyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">Nwi_1506</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Condenses choline with CDP-diglyceride to produce phosphatidylcholine and CMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 17">
            <text>a CDP-1,2-diacyl-sn-glycerol + choline = a 1,2-diacyl-sn-glycero-3-phosphocholine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14597"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 17">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 17">Cell inner membrane</location>
            <topology evidence="4 17">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 17">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.24" evidence="6 17"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314773.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1506"/>
    <dbReference type="EnsemblBacteria" id="ABA04767">
        <property type="protein sequence ID" value="ABA04767"/>
        <property type="gene ID" value="Nwi_1506"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1506"/>
    <dbReference type="eggNOG" id="COG1183">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086279_0_0_5"/>
    <dbReference type="OMA" id="FLHPFRV"/>
    <dbReference type="OrthoDB" id="1232102at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050520">
        <property type="term" value="F:phosphatidylcholine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026027">
        <property type="entry name" value="PcS"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000851">
        <property type="entry name" value="PcS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="17" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 17" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="13 17" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="15 17" id="KW-0464">Manganese</keyword>
    <keyword evidence="14 17 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="17" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 17 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="11 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="139"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="192"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="215"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00015623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00033321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ABA04767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="244" mass="26325" checksum="E4096C0590490B2B" modified="2005-10-11" version="1">MSQPSRQGPLPGPGTSRTAALLVHVFTATGAGIALLAMLEAVREHWAAMFAWLGLALLIDGLDGPMARRLNVQAAQPNWSGEILDLVVDFLTYVFVPAYAITASGLLLPFAAPLLGVGIVISSALYFSDRRMKTDDNHFRGFPALWNAAAFYLFLLRPSPAASTLAIAALIVLTFVPLKVLHPVRVKRFRGFNLSLIAVWAALAMTTVAHDFNVAMPVTAVLCAIAFYIVCGDAVIRLLRPEKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSL1</accession>
    <name>Q3SSL1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1469</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04730">
        <property type="protein sequence ID" value="ABA04730"/>
        <property type="gene ID" value="Nwi_1469"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1469"/>
    <dbReference type="eggNOG" id="ENOG5030X5N">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127514_0_0_5"/>
    <dbReference type="OMA" id="EYWHALL"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="137" mass="16101" checksum="C49597ADCF6603F4" modified="2005-10-11" version="1">MCNWSAYWRMPSARQMEKLAREQRQDEPNSEPDGWWHSVLNDPRATGAPRLPIHQLRLNEIPRDVLRVECLRCTRIVEIQKADAVRLYGPHAIWKDVGRKLLDDGCKYRTGRHEEDGCWPDWRLESASIASELRILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="130" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSN4</accession>
    <name>Q3SSN4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Histidine kinase</fullName>
            <ecNumber evidence="4">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Nwi_1446</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314715.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1446"/>
    <dbReference type="EnsemblBacteria" id="ABA04707">
        <property type="protein sequence ID" value="ABA04707"/>
        <property type="gene ID" value="Nwi_1446"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1446"/>
    <dbReference type="eggNOG" id="COG5000">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019564_1_0_5"/>
    <dbReference type="OMA" id="SKFARMP"/>
    <dbReference type="OrthoDB" id="692375at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017232">
        <property type="entry name" value="NtrY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045671">
        <property type="entry name" value="NtrY-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19312">
        <property type="entry name" value="NtrY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037532">
        <property type="entry name" value="STHK_NtrY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="26"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="99"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="297"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="12">
        <location>
            <begin position="323"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="11">
        <location>
            <begin position="388"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="10">
        <location>
            <begin position="513"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="738"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="745"/>
            <end position="766"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA04707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="766" mass="83662" checksum="F78A596BBB038AE7" modified="2005-10-11" version="1">MTTADTPVPSHDPTFAESRGWAMRRLLAPVAIGLALLSAFLTFVVLVGLTPIEPTHRVVITFLLVDAGIILLLVGIIAREVWRVVQARRRGRAAAKLHVQVVGLFSIIAVLPAVVVSVVASVTIDRGLDRLFSGPIKEVIENSRIVAHAYANEHAQLIRGDILGMANDVSNARPLYDQDRVTFLQLLTSSAKARNLPGAMLIDKEGHLLVSAQTGIKRAYDTPNPDILKNVNEDDPQIALIPEANYVAAVIRLRAFQDTFLYVARLLDPYVVSQLKQTESSIVEYATIEARRLGIQVALALMFAVIALTILMASVLLGLNFADRFVAPVRRLMNAANLVSTGDLNVQVPVLKSEGDLAQLGETFNKMTAELRTQRDELVNASGLIDSRRRFIEAVLSSASAGIIGVDGANTIRILNRSAEKLIGHSESETLGHPLSEVIPELSDLMQTAREGAQRLVQGQITLNRDGNERNISVRVSAEQTSQSRDSYIITLDDITELVTAQRTSAWGDVARRIAHEIKNPLTPIQLSAERIRRKFGKVITQDKPIFEQCTDTIVRQVEDIRRMVDEFSRFARMPKPVMEGEDVADTVRQTVFLMRVGHPEIDIEADIRQEPMRAQFDRRLISQALTNIIKNAAEAIEAVPPEVLGKGRIDVIAAQEEDDIIIDVIDNGVGLPKVARARLLEPYVTTREKGTGLGLAIVGRVLEDHGGKLELNDASAIRPEARGAWMRLRFAATGPANAGTIGDDGATNGDTLTGKETKTEVATGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSS6</accession>
    <name>Q3SSS6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ubiquinol-cytochrome C chaperone</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1404</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0174 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1404"/>
    <dbReference type="EnsemblBacteria" id="ABA04665">
        <property type="protein sequence ID" value="ABA04665"/>
        <property type="gene ID" value="Nwi_1404"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1404"/>
    <dbReference type="eggNOG" id="COG5452">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051390_5_1_5"/>
    <dbReference type="OMA" id="IVDAFFQ"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021150">
        <property type="entry name" value="Ubiq_cyt_c_chap"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007129">
        <property type="entry name" value="Ubiqinol_cyt_c_chaperone_CPB3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014569">
        <property type="entry name" value="Ubq_cyt-c_CBP3-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12184">
        <property type="entry name" value="PTHR12184"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03981">
        <property type="entry name" value="Ubiq_cyt_C_chap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032079">
        <property type="entry name" value="UCP032079"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiq_cyt_C_chap" evidence="2">
        <location>
            <begin position="58"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="207" mass="23167" checksum="4065CE428888E060" modified="2005-10-11" version="1">MIPECNNGSPLRVVFGTHMRWPFNRFKKQSPAPSRRTIEAIYGMIVTQAREPAFYQVYGVPDTVDGRFDMVLLHLWMVLRRLRSGPDKALPQALFDHFCSDMDANLREMGVGDLSVPKRMQAFGEAFYGRSAAYDRALDEGLEPLARALDKNIYNGGNAESARRLADYATEALAALACDDRAGPTEDLTFGRFAFPDLAQREQKSGP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSV6</accession>
    <name>RL14_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplN</name>
        <name type="ordered locus">Nwi_1374</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Forms a cluster with proteins L3 and L19. In the 70S ribosome, L14 and L19 interact and together make contacts with the 16S rRNA in bridges B5 and B8.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009797128.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSV6"/>
    <dbReference type="SMR" id="Q3SSV6"/>
    <dbReference type="STRING" id="323098.Nwi_1374"/>
    <dbReference type="EnsemblBacteria" id="ABA04635">
        <property type="protein sequence ID" value="ABA04635"/>
        <property type="gene ID" value="Nwi_1374"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1374"/>
    <dbReference type="eggNOG" id="COG0093">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095071_2_1_5"/>
    <dbReference type="OMA" id="AKEVLCI"/>
    <dbReference type="OrthoDB" id="1799923at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.150.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01367">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036853">
        <property type="entry name" value="Ribosomal_L14_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000218">
        <property type="entry name" value="Ribosomal_L14P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005745">
        <property type="entry name" value="Ribosomal_L14P_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019972">
        <property type="entry name" value="Ribosomal_L14P_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761">
        <property type="entry name" value="PTHR11761"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00238">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01374">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50193">
        <property type="entry name" value="SSF50193"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01067">
        <property type="entry name" value="rplN_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00049">
        <property type="entry name" value="RIBOSOMAL_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000266513" description="50S ribosomal protein L14">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01367"/>
        </source>
    </evidence>
    <sequence length="122" mass="13420" checksum="CE60096141C94862" modified="2005-10-11" version="1">MIQMQTNLDVADNSGARRVMCIKVLGGSKRRYATVGDIIVVSIKEAIPRGKVKKGDVMKAVVVRVSKDIRRADGSVIRFDRNAAVLINNQSEPVGTRIFGPVPRELRAKNHMKIISLAPEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST78</accession>
    <name>Q3ST78_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Restriction endonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1252</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1252"/>
    <dbReference type="EnsemblBacteria" id="ABA04513">
        <property type="protein sequence ID" value="ABA04513"/>
        <property type="gene ID" value="Nwi_1252"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1252"/>
    <dbReference type="eggNOG" id="COG3440">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077422_0_0_5"/>
    <dbReference type="OMA" id="AHIRPYG"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003615">
        <property type="entry name" value="HNH_nuc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13391">
        <property type="entry name" value="HNH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HNHc" evidence="1">
        <location>
            <begin position="203"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="306" mass="35041" checksum="CC5424CB3AC2422F" modified="2005-10-11" version="1">MAINLVIAVTDGDWFDMLRQQPNLAEVNFWAPSAANFRALQPGEMFLFKLHAPRNVIVGGGIFAYANALPCSLAWEAFREANGARSLPEMRARIARYRRADPADRSDFEIGCRILTQPFFFEEADWIPVPASWAPNIVSFKTYNTGDLEGLALWETVTERMNRPRFPGMAEAQTRFGEPHLIRPRLGQGAFRVLVTDNYNRRCAITQERTLPALEAAHIRPYGDGGDHEARNGLLLRRDIHSLFDAGYVTVTPELRFEVSRRIKEEFNNGRHYYEHHGKPIFAPADNLRRPDPEALRWHNEHVFRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STB1</accession>
    <name>Q3STB1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Tripartite ATP-independent periplasmic transporter, DctQ component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_1218</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314509.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1218"/>
    <dbReference type="EnsemblBacteria" id="ABA04480">
        <property type="protein sequence ID" value="ABA04480"/>
        <property type="gene ID" value="Nwi_1218"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1218"/>
    <dbReference type="eggNOG" id="COG4665">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086356_2_2_5"/>
    <dbReference type="OMA" id="LTWLCWP"/>
    <dbReference type="OrthoDB" id="2073326at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007387">
        <property type="entry name" value="TRAP_DctQ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35011">
        <property type="entry name" value="PTHR35011"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04290">
        <property type="entry name" value="DctQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="91"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="134"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA04480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="179" mass="20208" checksum="464EAB43E633840C" modified="2005-10-11" version="1">MRLLLALSTAIDRLNEKVGYVCNILVLLAVLVSAGNAMIRYAFGYSSNSWLELQWYMFAILVMFGASYTFKRNEHVRVEIFYLILSDRGRLWLDLIGTLFFLIPACLLLSYLSWPFFMQSYAIGEVSGNAGGLVRWPIKLVVPLGFVMLAIQGISEVIKRIAALQGLVTIQATYERPTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STI1</accession>
    <name>Q3STI1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1148</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314440.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1148"/>
    <dbReference type="EnsemblBacteria" id="ABA04410">
        <property type="protein sequence ID" value="ABA04410"/>
        <property type="gene ID" value="Nwi_1148"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1148"/>
    <dbReference type="eggNOG" id="COG5436">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113999_0_0_5"/>
    <dbReference type="OMA" id="LMTEAQH"/>
    <dbReference type="OrthoDB" id="1534577at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014456">
        <property type="entry name" value="UCP010244_IM"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010244">
        <property type="entry name" value="UCP010244_imp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="192" mass="20596" checksum="44187CA5BA32CB51" modified="2005-10-11" version="1">MIRLLFAIVGGILLGGIVHLVSVLALPRIASQDAYSRLTEIATLNAVTPLPPAEPANSLMPYMDPAFAVAICRYDLSDGSIKLTAPVSEAYTSVSFYTQSNVAYYAINDRSAGRKVIELNLMTEAQHAELPEEEDVTAADRLIIDSPSTTGLIVLKALAPEPGMMDQARSSLAAAKCRLQTEPPVVKPEAKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STJ3</accession>
    <name>Q3STJ3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Flagellar biosynthetic protein FliR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_1136</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Role in flagellar biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Bacterial flagellum basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the FliR/MopE/SpaR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314429.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1136"/>
    <dbReference type="EnsemblBacteria" id="ABA04398">
        <property type="protein sequence ID" value="ABA04398"/>
        <property type="gene ID" value="Nwi_1136"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1136"/>
    <dbReference type="eggNOG" id="COG1684">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063626_3_0_5"/>
    <dbReference type="OMA" id="APQFNIF"/>
    <dbReference type="OrthoDB" id="1942709at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044780">
        <property type="term" value="P:bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006303">
        <property type="entry name" value="FliR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002010">
        <property type="entry name" value="T3SS_IM_R"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30065">
        <property type="entry name" value="PTHR30065"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01311">
        <property type="entry name" value="Bac_export_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00953">
        <property type="entry name" value="TYPE3IMRPROT"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01400">
        <property type="entry name" value="fliR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="5 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="11" id="KW-0969">Cilium</keyword>
    <keyword evidence="11" id="KW-0282">Flagellum</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="127"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA04398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="256" mass="27073" checksum="BDE948F661EA5B1E" modified="2005-10-11" version="1">MRIDISLLPALGAAFMLVFARVGAMVMLLPGFGEGYIPVRVKLAIALLLTFIILPLHRAAYHVDMQSVAPLAVLLVHEIIIGVVLGATARVTLAALHVAGSVIAQQLGLGFVTSVDPTQGQQGALMGNFLTLLGIALLFATDTHYLVIAALNDSYKIFSPGELMPSGDVAALATRAFAGAFKIGLQLSAPFLVFGLVFNIGLGVLARLMPQMQVYFVGVPLSIFIGFMIFAAVLASMMGTYMDYLIGVMQDLTSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STM1</accession>
    <name>Q3STM1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314401.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1108"/>
    <dbReference type="EnsemblBacteria" id="ABA04370">
        <property type="protein sequence ID" value="ABA04370"/>
        <property type="gene ID" value="Nwi_1108"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1108"/>
    <dbReference type="eggNOG" id="ENOG503392Y">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_163378_0_0_5"/>
    <dbReference type="OMA" id="WQLKARA"/>
    <dbReference type="OrthoDB" id="1931970at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035924">
        <property type="entry name" value="FlaG-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160214">
        <property type="entry name" value="SSF160214"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="121" mass="13199" checksum="CC4A78840296EC30" modified="2005-10-11" version="1">MSADFSIKPIGTPVAAPVVQLASAAANDTVATELPPSQSVTAADISTRSRNDLQTSREALSRQVVVDRDAAAVVYQVVDRRTSLVVKQFPEEAMLRRRAYFRTLDLMNAERDPPAATDRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STP5</accession>
    <name>Q3STP5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1084</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314380.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1084"/>
    <dbReference type="EnsemblBacteria" id="ABA04346">
        <property type="protein sequence ID" value="ABA04346"/>
        <property type="gene ID" value="Nwi_1084"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1084"/>
    <dbReference type="eggNOG" id="ENOG503362H">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034357_0_0_5"/>
    <dbReference type="OrthoDB" id="1322628at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="385" mass="41089" checksum="EBB4F885D5DB014C" modified="2005-10-11" version="1">MVIVTATRNDLRDEFAMADEAFALSPISASAAPLGEADFDAIREAFMETSRGRWFLAEYARRNRSADTAMVLDAVARIEQTIETQQQAAPADALPKALAAIRKSLIEARTTATAALDKRVMNDALVPIHNGVRIIREISWRWREIGGDSRICDILDSQADAIEKAHSQLAARDDMAALHSAFSVIETTLDELWNCPAPTAPPSRSDAAPPETINEIVALPEEAASHAQAVAEVIASEPEKAAIARDAPVDATGESMTTNHGEDAHAAGLSEQDTAHDDAVLDMIALEMGAPDLEEPETGLVSTGLASIERPETPDLVPDPEPIADNLSEHPDREPEPEANSVNPAPASLGASLLASGVVSSPTPRSDPMAPFKRMTQAEKIAFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="135" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STT2</accession>
    <name>Q3STT2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase</fullName>
            <ecNumber evidence="6 7">6.3.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">D-alanyl-D-alanine-adding enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">murF</name>
        <name evidence="11" type="ordered locus">Nwi_1047</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>ATP + D-alanyl-D-alanine + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate = ADP + H(+) + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine</text>
            <dbReference type="Rhea" id="RHEA:28374"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57822"/>
            <dbReference type="ChEBI" id="CHEBI:61386"/>
            <dbReference type="ChEBI" id="CHEBI:83905"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6 7">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MurCDEF family. MurF subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.10" evidence="6 7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314343.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1047"/>
    <dbReference type="EnsemblBacteria" id="ABA04309">
        <property type="protein sequence ID" value="ABA04309"/>
        <property type="gene ID" value="Nwi_1047"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1047"/>
    <dbReference type="eggNOG" id="COG0770">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031507_4_1_5"/>
    <dbReference type="OMA" id="VEMGANH"/>
    <dbReference type="OrthoDB" id="1861122at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047480">
        <property type="term" value="F:UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008766">
        <property type="term" value="F:UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02019">
        <property type="entry name" value="MurF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000713">
        <property type="entry name" value="Mur_ligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035911">
        <property type="entry name" value="MurE/MurF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005863">
        <property type="entry name" value="UDP-N-AcMur_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01225">
        <property type="entry name" value="Mur_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63418">
        <property type="entry name" value="SSF63418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01143">
        <property type="entry name" value="murF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1 6 7" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 6 7" id="KW-0133">Cell shape</keyword>
    <keyword evidence="5 6 7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 6 7" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase" evidence="8">
        <location>
            <begin position="28"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_M" evidence="10">
        <location>
            <begin position="113"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_C" evidence="9">
        <location>
            <begin position="337"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="115"/>
            <end position="121"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA04309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="476" mass="49577" checksum="05576C1C992CC9E0" modified="2005-10-11" version="1">MSARPLWTLDEMAAAMRAEGRGTLPAAITGISIDSRTVAPGEAYFAIKGDVHDGHAFVGAALKAGAALAVVEAARRDQFALELPLLVVDDVLAALRDLARASRARLAAQVIAVTGSVGKTSTKEALRGVLGAQGKTHVSVASFNNHWGVPLSLSRCPADARFAVFEIGMNHAGEITPLVKMVRPHVAIITTVEPVHLEFFSGVEAIADAKAEIFEGVEPDGAVVLNRDNAQFARLQECARKLGIAKIVTFGADEKADARLLDLALHPAGSAVHAEILGSELTYKLGMPGRHMAMNSLAVLAAASLAGADLALAGLALSQAQPAAGRGVRRALAVRGGEALLIDESYNANPASMAAALNVLGEAKVGTQGRRIAVLGDMLELGPRSADLHRDLTGAIDANDVDMIFCCGPLMRNLWNALSSGRRGGYAEDAAALESQVTAAIRSGDVIMVKGSLGSRMKVIVTALERRYSGSAAEIL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-02-21" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STT6</accession>
    <name>RSMH_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal RNA small subunit methyltransferase H</fullName>
            <ecNumber evidence="1">2.1.1.199</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">16S rRNA m(4)C1402 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">rRNA (cytosine-N(4)-)-methyltransferase RsmH</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rsmH</name>
        <name type="synonym">mraW</name>
        <name type="ordered locus">Nwi_1043</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>cytidine(1402) in 16S rRNA + S-adenosyl-L-methionine = H(+) + N(4)-methylcytidine(1402) in 16S rRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42928"/>
            <dbReference type="Rhea" id="RHEA-COMP:10286"/>
            <dbReference type="Rhea" id="RHEA-COMP:10287"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74506"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.199"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. RsmH family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.199" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314339.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3STT6"/>
    <dbReference type="SMR" id="Q3STT6"/>
    <dbReference type="STRING" id="323098.Nwi_1043"/>
    <dbReference type="EnsemblBacteria" id="ABA04305">
        <property type="protein sequence ID" value="ABA04305"/>
        <property type="gene ID" value="Nwi_1043"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1043"/>
    <dbReference type="eggNOG" id="COG0275">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038422_1_1_5"/>
    <dbReference type="OMA" id="NPAKRTF"/>
    <dbReference type="OrthoDB" id="1272633at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071424">
        <property type="term" value="F:rRNA (cytosine-N4-)-methyltransferase activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01007">
        <property type="entry name" value="16SrRNA_methyltr_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002903">
        <property type="entry name" value="RsmH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023397">
        <property type="entry name" value="SAM-dep_MeTrfase_MraW_recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11265">
        <property type="entry name" value="PTHR11265"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01795">
        <property type="entry name" value="Methyltransf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004486">
        <property type="entry name" value="MraW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF81799"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00006">
        <property type="entry name" value="TIGR00006"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000223549" description="Ribosomal RNA small subunit methyltransferase H">
        <location>
            <begin position="1"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="36"/>
            <end position="38"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01007"/>
        </source>
    </evidence>
    <sequence length="332" mass="35176" checksum="081F460D7BB6F072" modified="2005-10-11" version="1">MTAPPIRHVPVLGREAVAMLAPRAGGVYVDATFGAGGYSRAILATAETRVIGIDRDPTAIAGGSGLVEESGGRLILVEDRFSHLAAVCAAQDAAAVDGVVMDVGVSSMQLDEAGRGFSFRLDGPLDMRMSGHGPTAAEVVARASETDLADIIYIFGEERRSRAVARAIVGARRHGPIATTRALADIVSKVVRAKPHEIHPATRTFQALRIFVNEELDELIAALAAAERVLKPGGRLAVVSFHSLEDRIVKNFLAWRGKTGGGSRHRPEIERAPPSFAILTKRPLTPGDDEVQANPRARSARLRAAERTDAAAIDDGAELPAWPSLANVMRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU00</accession>
    <name>Q3SU00_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Pirin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0979</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the pirin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314280.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0979"/>
    <dbReference type="EnsemblBacteria" id="ABA04241">
        <property type="protein sequence ID" value="ABA04241"/>
        <property type="gene ID" value="Nwi_0979"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0979"/>
    <dbReference type="eggNOG" id="COG1741">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064194_2_2_5"/>
    <dbReference type="OMA" id="NLRVWND"/>
    <dbReference type="OrthoDB" id="729805at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012093">
        <property type="entry name" value="Pirin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003829">
        <property type="entry name" value="Pirin_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041602">
        <property type="entry name" value="Quercetinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43212">
        <property type="entry name" value="PTHR43212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02678">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17954">
        <property type="entry name" value="Pirin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006232">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pirin" evidence="3">
        <location>
            <begin position="10"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Pirin_C_2" evidence="4">
        <location>
            <begin position="151"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="232" mass="24819" checksum="B23CFF3360DF0363" modified="2005-10-11" version="1">MIELRPFNKLGGADHGWLKAKHHFSFASYYDPGNVGHGSLRVWNDDEIAPNSGFPPHPHANMEIITYVREGAITHQDSMGNKGRTEAGDVQVMSAGSGVRHSEYNLEPVKTTLFQIWIEPTKDGGEPAWGAKPFPKGDRSGRLVTLASGFKADSDALPIRADARVLATTLKAGESASYAAGKTRNLYLVPAVGAVEVNGVRVNARDGAAIRDEATVSITALEDSEVVLVDAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="127" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU17</accession>
    <name>Q3SU17_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Type I secretion system ATPase, PrtD</fullName>
            <ecNumber evidence="14">3.6.3.25</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Nwi_0962</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in beta-(1--&gt;2)glucan export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.25" evidence="14"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314265.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0962"/>
    <dbReference type="EnsemblBacteria" id="ABA04224">
        <property type="protein sequence ID" value="ABA04224"/>
        <property type="gene ID" value="Nwi_0962"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0962"/>
    <dbReference type="eggNOG" id="COG4618">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_95_6_5"/>
    <dbReference type="OMA" id="LDSMSRN"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030256">
        <property type="term" value="C:type I protein secretion system complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030253">
        <property type="term" value="P:protein secretion by the type I secretion system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010128">
        <property type="entry name" value="ATPase_T1SS_PrtD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24222:SF0">
        <property type="entry name" value="PTHR24222:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01842">
        <property type="entry name" value="type_I_sec_PrtD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="118"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="425"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="459"/>
            <end position="466"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABA04224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="668" mass="71285" checksum="5CC6B140FF6829D2" modified="2005-10-11" version="1">MSLDDNRGNSNSSSGSEDRAEPVISPAERDSNDQSDLASPVKPSETLDGRAEEVSPPVAADGKGADAPLPTAGGDHRGGKGNLNGGGGRGPQLHKRSGDNEFRDVLGKGLAAARQNLVTVGIFSIVVNLLVLSIPIYLFNMSDRVLTSRSVDTLIMLSSIVVMAIAAHVLMDMLRRFILMRVAVETEARLGGPVLSAAAKAAQSGSSREFQTLADLQHLRAFITGPVLLTIFDAPVAPAYLAVVFLINPQLGLIVLASSASLIGVALINQRVTAVPFTRANAFGTRANLQAEAMARNAQVINAMGMIPEGVQIWGQETVESLKAQVAGHDLNIIMTGISKFLRLGTQIGILGWGAYLALESELTGGMIIAASIVASRALAPLEGTIEGWRQFVQARSAYSRIKSLLQSSPLNLERLRLPRPEGRLSVERILYVPPPNKKVILNGISFQLEPGESLAIVGPSGTGKTMLARMLVGSIIPTAGNVRLDMMDLRNWDPRQFGESVGYLPQEVQLFPASIKANIARMRADAVDEDIFDAAETADVHEMISEMAQGYETVIGMDGSPLSGGQRQRIGLARAFYGNPRLIVLDEPNANLDASGERALAKALVRAKEKRMTVVTITQRPALLQSVDKIMILRNGAVQAFGARDEVIPLMSGRKRPSPSAAPMLDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU23</accession>
    <name>Q3SU23_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0956</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314259.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0956"/>
    <dbReference type="EnsemblBacteria" id="ABA04218">
        <property type="protein sequence ID" value="ABA04218"/>
        <property type="gene ID" value="Nwi_0956"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0956"/>
    <dbReference type="eggNOG" id="COG3513">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2383211_0_0_5"/>
    <dbReference type="OrthoDB" id="24005at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="94" mass="10728" checksum="72E7E96A1B5E7A2E" modified="2005-10-11" version="1">MEVFETVDGKWNGEAVRRFDANKKNAGPRISHTPLWRSVNDGAKLVMRIHKGDLIRLDHDGRTRIMVVHRLDAAAGRFKLAEHNETKSRQASCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU38</accession>
    <name>Q3SU38_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">TonB-dependent receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_0941</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Cell outer membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TonB-dependent receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314244.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0941"/>
    <dbReference type="EnsemblBacteria" id="ABA04203">
        <property type="protein sequence ID" value="ABA04203"/>
        <property type="gene ID" value="Nwi_0941"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0941"/>
    <dbReference type="eggNOG" id="COG4771">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008287_8_2_5"/>
    <dbReference type="OMA" id="VPYDGYV"/>
    <dbReference type="OrthoDB" id="71288at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039426">
        <property type="entry name" value="BtuB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012910">
        <property type="entry name" value="Plug_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037066">
        <property type="entry name" value="Plug_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000531">
        <property type="entry name" value="TonB-dep_rcpt_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036942">
        <property type="entry name" value="TonB_rcpt_b-brl_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30069">
        <property type="entry name" value="PTHR30069"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07715">
        <property type="entry name" value="Plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00593">
        <property type="entry name" value="TonB_dep_Rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0798">TonB box</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229122" evidence="7">
        <location>
            <begin position="29"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="domain" description="Plug" evidence="9">
        <location>
            <begin position="63"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="TonB_dep_Rec" evidence="8">
        <location>
            <begin position="253"/>
            <end position="714"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA04203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="716" mass="79581" checksum="A8FEB0259CBC460F" modified="2005-10-11" version="1">MILSMNCMSRGALLSSVAIFAMTAGAEAQDRDQSRDSVYQLGEIYVSGGGRSGSGSGGGVGGSTVTREQTWTFEKQTLDQAVNLVPGVNGTQSANGQRNETDIFVRGFGRWQVPLMIDGVRVYLPADNRLDFSRFLTGDIAEIQIQKGYASVLDGPGGMGGAINLVSIKPVKAFESELNAGTMFDNRGAFQGWNSYARVGSRQEKFYVQGSVNQLDQDFWSLSRGYSPYPAQGPYAGSLEDGGRRNGSDTRDWRINLKAGYTPNETDEYSINYIKQSGAKGAPLNVYNNPPSSQNSFWLWPKWDVENLSFLSNTQLGDASYVRTKLYYNTFYNLLSAYDDITYSSQSNNGRFNSYYDDSARGGSVEAGTELIPMNTLKTAFHYRQDFHAEWNHNRPTHPTMAMLEPRQNQAQTTWSIAGEDTFHVTPDFDLVAGISYDKYSINRAEEWDSGGGFIYEYPKGGSDALNWQTAAIWRYNETGQVHFSVSDRTRFPTIFELYSQRFGTAIPNPNLGPERATNYELGWKGQIASNVKGTAAIFYSDVRDLIQAVRVSPTQSQTQNINQGHFYGFEASFEAQLSAQFMIGGNYTFIERNVSDVSVPGFQPVGVPTQKAFLYATWRPIEPLAITPNLEVTSDRWSDVLVGTQGPNAPIRAAFPDLYIRTGAYTLANINVNYKFTENVEVAGGVRNLLDQNYELAWGLPQPGRTFYLKARARF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="135" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU68</accession>
    <name>Q3SU68_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Heavy metal translocating P-type ATPase</fullName>
            <ecNumber evidence="9">3.6.3.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_0911</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.4" evidence="9"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0911"/>
    <dbReference type="EnsemblBacteria" id="ABA04173">
        <property type="protein sequence ID" value="ABA04173"/>
        <property type="gene ID" value="Nwi_0911"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0911"/>
    <dbReference type="eggNOG" id="COG2217">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001771_0_3_5"/>
    <dbReference type="OMA" id="MAKDPIC"/>
    <dbReference type="OrthoDB" id="237367at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.620.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045800">
        <property type="entry name" value="HMBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012348">
        <property type="entry name" value="RNR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011017">
        <property type="entry name" value="TRASH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007029">
        <property type="entry name" value="YHS_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19335">
        <property type="entry name" value="HMBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04945">
        <property type="entry name" value="YHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00746">
        <property type="entry name" value="TRASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="SSF81653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="433"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="461"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="777"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="802"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="domain" description="TRASH" evidence="8">
        <location>
            <begin position="73"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="37"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA04173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="831" mass="86983" checksum="3530D3F811120657" modified="2005-10-11" version="1">MNTAGRPGSKSGNGCGCSGEKQAASPAAASPESSCCGDREATHDHHAHDHDSDNHHEQHHGHADAMTDGKAVDPVCGMTVDPRTSKHRFDYQDTTYHFCCAGCRTKFAAAPRSYLDKSRAPPPADLPEGTIYICPMDPEVRQVGPGACPICGMALEPELVSLDDAPNPELANMSRRFWIGLILALPVITLDMGAHIAGAHGWIDQTLSNWIQLAFATPVVFWAGWPFFVRGWQSLTTRNLNMFTLIAMGTGVAYLYSLAATMTPAIFPPAFRGADGAVAVYFEAAAVITILVLLGQVLELRAREATSGAIKALLDLAPKTARRVDEQGADHDVALGEVAVGDRLRVRPGEKVAVDGLIIEGRSSLDESMVTGESMPVAREAGSKVIAGAINRSGSFIMRAEKVGRDTLLSRIVQMVAQAQRSRAPIQRLADRVAGWFVPAVIAAAVVAFAVWSFVGPEPRLAFGLVAAVSVLIIACPCALGLATPMSIMVGVGRGAQAGVLIRNAEALERMEKVDTLVVDKTGTLTEGKPKVVAVEAVEGIEKDDLLRLAATVERSSEHPLADAIVAAATERGLALGEVQEFDAPAGKGVIGKVAGKTVLVGSAAFLESRNVAVRDMAPLADDLRDDGATVVNVAFDGRLAGLLAIADPVKASTRAALKALAGDGIRVIMLTGDNRTTANAIARQLGIADVEADVLPEQKSAVVARLQNEGRVVAMAGDGINDAPALAAADVGIAMGTGTDIAMESAGVTLLGGDLTGLVRARRLSEATMRNIRQNLFFAFMYNAAGIPIAAGVLYPVFGLLLSPIVAAAAMALSSVSVVGNALRLRATRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU86</accession>
    <name>Q3SU86_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alkyl hydroperoxide reductase C</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Peroxiredoxin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Thioredoxin peroxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_0891</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0891"/>
    <dbReference type="EnsemblBacteria" id="ABA04155">
        <property type="protein sequence ID" value="ABA04155"/>
        <property type="gene ID" value="Nwi_0891"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0891"/>
    <dbReference type="eggNOG" id="COG0450">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_4_4_5"/>
    <dbReference type="OMA" id="VRHTTCN"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019479">
        <property type="entry name" value="Peroxiredoxin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10417">
        <property type="entry name" value="1-cysPrx_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 9" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="8">
        <location>
            <begin position="19"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate" evidence="7">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA04155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="212" mass="23527" checksum="ED14B38212B8BB6F" modified="2005-10-11" version="1">MSLLMNDERPRLTPSPFPLRMGDAAPDFEARSTKGPIRLSDYRGRWLVFFSHPADFTPVCTTEFVALAKAQDRFAALDCALLGLSVDSLYSHLAWTRTITELFAVEIPFPVIEDPSMMVGRAYGMIDEAAESSASVRATYFIDPEGVIRAITHYPLTIGRSIDEMVRMVAALQATASSQKLAPANWRPGEPLLLPADEKTQKDVNWFCRSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUE3</accession>
    <name>Q3SUE3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cobyrinic acid a,c-diamide synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0833</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0833"/>
    <dbReference type="EnsemblBacteria" id="ABA04098">
        <property type="protein sequence ID" value="ABA04098"/>
        <property type="gene ID" value="Nwi_0833"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0833"/>
    <dbReference type="eggNOG" id="COG1192">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037612_4_0_5"/>
    <dbReference type="OMA" id="GISPMTE"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_31" evidence="1">
        <location>
            <begin position="66"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="350" mass="39188" checksum="82D0ED1C5F170F5B" modified="2005-10-11" version="1">MIPDDELVGINEIAEMAKTSRQAVANWRTRMSDFPKPIVDLASGPVFRRSHIRAWLRRRKVPMAHVFSTINLKGGVGKTTTTVALAETLSAEKRKKVLVIDLDPQTNATTMLIGEEKWRELNDKGFTLAQLFKDALNPDSKKFDLKKTLQKRVSDVSSATTIDLLPSSLDLIDVQDELINTPVGKYGAIRPFDILWRATKDLIEDYDVVIIDCPPNLGKITQNGLRMSHGFIIPTIPDILSTYGIPQIVRRVREFSEEIAEDIEPLGIVVTKFQANSNVHVNMLKQLKLNHTADAKNWPPVFDTLIPQANQIAAAAEHSGYSTLKQKWGYQGLHDRFSDLATEILTKLGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUG1</accession>
    <name>Q3SUG1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0815</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the N(4)/N(6)-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314128.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0815"/>
    <dbReference type="REBASE" id="11316">
        <property type="entry name" value="M.NwiORF815P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04080">
        <property type="protein sequence ID" value="ABA04080"/>
        <property type="gene ID" value="Nwi_0815"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0815"/>
    <dbReference type="eggNOG" id="COG0863">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_902636_0_0_5"/>
    <dbReference type="OMA" id="VLCEIAY"/>
    <dbReference type="OrthoDB" id="1487464at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006306">
        <property type="term" value="P:DNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002941">
        <property type="entry name" value="DNA_methylase_N4/N6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001091">
        <property type="entry name" value="RM_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01555">
        <property type="entry name" value="N6_N4_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00508">
        <property type="entry name" value="S21N4MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N6_N4_Mtase" evidence="6">
        <location>
            <begin position="91"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="308" mass="33392" checksum="C547AD863A10FFEE" modified="2005-10-11" version="1">MAKGLARTFGQDLMRGEHEVGATTNGGVLMPSHTSGDPGFYSKKRAREAEIGRELTTEEFLADHYEASDAPTASGTSIFDPVLCEIAYRWFCPQGGTVLDPFAGGSVRGIVASRLGRRYVGIELRHEQVEANRAQVTIAVEPSPEWRVGDARDLGAIAADVAADLIFSCPPYWNLERYSDDPADLSTMGEAAFFEAQAAIIAAAVARLKDDRFAVWVVGDVRDDRGFYVNLPGRTVEAFEAAGARFYNEAILVTAVGSLPIRTGRQFTAARKLGRTHQSVLVFVKGDPRRATEACGEVEFGEIEEALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUG8</accession>
    <name>Q3SUG8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phage portal protein, lambda</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0808</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314121.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0808"/>
    <dbReference type="EnsemblBacteria" id="ABA04073">
        <property type="protein sequence ID" value="ABA04073"/>
        <property type="gene ID" value="Nwi_0808"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0808"/>
    <dbReference type="eggNOG" id="COG5511">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027870_3_1_5"/>
    <dbReference type="OMA" id="KKIEACF"/>
    <dbReference type="OrthoDB" id="274441at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019068">
        <property type="term" value="P:virion assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006429">
        <property type="entry name" value="Phage_lambda_portal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05136">
        <property type="entry name" value="Phage_portal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01539">
        <property type="entry name" value="portal_lambda"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="476"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="511" mass="55793" checksum="D1BF3A23CAD6E160" modified="2005-10-11" version="1">MAANWIDRALASVAPGAARKRLLERQAFEKLARAYDGAAVGRRTDGWRSSSSSADGEIASGASRLRDRMRDLTRNNPHAAKAVAVLVNNIVGAGIRPRAAAGTDALDNRINELWEAWAARSDADGLADFHGLTTLAVREMIEGGDVFLRRRIRRTEDKLPVPLQLQLLEADHLDDTKIAALPDGGRIVRGIEYDAIGRRRAYWLFPDHPGDTSVPLSRSLTSARVPADGIAHLFERQRVQSRGVPWGAPAMRAMRDLDDWTNAELVRKKTEACLVGVVLGADEADQGVAPTVVDAEGKTIEQFEPGLIAYARGGKDIKFNQPASTAGVSEWLRAQLHIIAAGYRVPYELLTGDLSQVNYSSLRGGLVEFRRMVDALQWQLVIPGFCEPVWRWFTEAAWVAGLIPNPVVKVEWQPPRFDAVDPLKDAQADLLMLRSGTMTLAQAIARQGYDPASQLGEIAEMNSALDRLKIVLDSDPRMMTNAGTAQPDPNDPAGDTADNEQPGKSKPKPGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUH4</accession>
    <name>Q3SUH4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0802</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0802"/>
    <dbReference type="EnsemblBacteria" id="ABA04067">
        <property type="protein sequence ID" value="ABA04067"/>
        <property type="gene ID" value="Nwi_0802"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0802"/>
    <dbReference type="eggNOG" id="ENOG502Z85X">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088242_0_0_5"/>
    <dbReference type="OMA" id="WSRAPKI"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045622">
        <property type="entry name" value="DUF6441"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20039">
        <property type="entry name" value="DUF6441"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="225" mass="24660" checksum="63B5166C0BC52372" modified="2005-10-11" version="1">MLHVRLLWTPVNSAPMRFAIQRPDLVEALAGTQKNIEHAVTAGMRDASEGLKADLREDVVAAGLGERLSRTWRGKTFPEAGESAEAAAYVWSRAPKIVDAFDRGVVIRSARGLFLAIPTATAGKTGRGAAGRLEKITPEGWQRRTGLKLRFVYRRGRPSLLVAENARVSKRGLAAANRRKTGQASVIVFVLVPQVALKKRLDIRQAAKRQAARVPSLIARHWSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUI2</accession>
    <name>Q3SUI2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative phage cell wall peptidase, NlpC/P60</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0794</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C40 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314107.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0794"/>
    <dbReference type="EnsemblBacteria" id="ABA04059">
        <property type="protein sequence ID" value="ABA04059"/>
        <property type="gene ID" value="Nwi_0794"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0794"/>
    <dbReference type="eggNOG" id="COG0791">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115301_1_0_5"/>
    <dbReference type="OMA" id="YSRDWGE"/>
    <dbReference type="OrthoDB" id="682655at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000064">
        <property type="entry name" value="NLP_P60_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011929">
        <property type="entry name" value="Phage_pept_NlpC/P60"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00877">
        <property type="entry name" value="NLPC_P60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02219">
        <property type="entry name" value="phage_NlpC_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51935">
        <property type="entry name" value="NLPC_P60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NlpC/P60" evidence="2">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="153" mass="17391" checksum="B55260C4206ADD5E" modified="2005-10-11" version="1">MSVRDHIIIEARSWIGTPYHHQAALKGVGCDCLGLVRGVWRAVYGADPEHPPAYSRDWAETLREETLADAASRHMIPLALDAIEPGDLLLFAINETAPAKHCAILVAPNRMIHTIESHPVAEVSFVPWWRNRLRFAFRFPKIRRPNRNNASRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUI6</accession>
    <name>Q3SUI6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314103.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0790"/>
    <dbReference type="EnsemblBacteria" id="ABA04055">
        <property type="protein sequence ID" value="ABA04055"/>
        <property type="gene ID" value="Nwi_0790"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0790"/>
    <dbReference type="HOGENOM" id="CLU_2094266_0_0_5"/>
    <dbReference type="OMA" id="IEFRERI"/>
    <dbReference type="OrthoDB" id="2176018at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="116" mass="13087" checksum="ADC9A4DB063C709F" modified="2005-10-11" version="1">MQPSIAIAPEALTITWVVVGSIILELFVLIVFLVRVSWWLSHRFTTIDANFLTVGKDVAQLKADISNDIAGRRVVAETRNDITELKTGFKDIRERVGRLEEHEDERRTFAPAAVPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUL7</accession>
    <name>Q3SUL7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0759</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314078.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04024">
        <property type="protein sequence ID" value="ABA04024"/>
        <property type="gene ID" value="Nwi_0759"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0759"/>
    <dbReference type="eggNOG" id="ENOG502ZKVI">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_650250_0_0_5"/>
    <dbReference type="OMA" id="FLRRHEF"/>
    <dbReference type="OrthoDB" id="1715955at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="422" mass="45743" checksum="E928B31EA8D4B675" modified="2005-10-11" version="1">MPETALSALVDVLSRPVHVPKPDQAGSLVIAEPPTPFVAPRFETADGDGGSLEKEIILIEASAAAGKSTLASQLSATLKIPVLDLARVPVAGGSLHALLAGLEAADKSDPMAAFHEGRLPLIVDALDEGRLLSNETGIEQFLGSSAELLQSDRRVASKPKLVMLGRFESIELARQQFGRAAPEVKHVTANVEFYDKEGARQLIHAYARAAARPGSHYLNHPDLAEDFITTFFDAIEAALGLSAGELWDDGRGKVFAGYAPLLAAVGSILAVIDDFDNVAKNLRANRGREAWGVVEAVLYEILCREQDKLRDRLSDQCESAVPNSVYDIEEQLTLLAQYIQSKPLEGAGRVRLSASDMGKYRAMVQTYLPHHPFLRRHEFGDAVLGANAVSRAVYHGGTISRDRLISLSRQWRQSGPTRGRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUP3</accession>
    <name>Q3SUP3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0732</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314052.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0732"/>
    <dbReference type="EnsemblBacteria" id="ABA03998">
        <property type="protein sequence ID" value="ABA03998"/>
        <property type="gene ID" value="Nwi_0732"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0732"/>
    <dbReference type="eggNOG" id="COG4679">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139003_0_0_5"/>
    <dbReference type="OMA" id="KLLYWEG"/>
    <dbReference type="OrthoDB" id="1733128at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2310.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009241">
        <property type="entry name" value="HigB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035093">
        <property type="entry name" value="RelE/ParE_toxin_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05973">
        <property type="entry name" value="Gp49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143011">
        <property type="entry name" value="SSF143011"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="117" mass="13300" checksum="B711A5E68D038879" modified="2005-10-11" version="1">MITFRETRPISWMKAARKTFEDFPQGAQDDILAALTVAADGSFPGNVKPMKGLGSGVYEVALRHRTDAYRAVYTLQFKDALWVVHAFQKKSKSGIKTPKQETDLILERIKRLKEMLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUV7</accession>
    <name>Q3SUV7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Amino acid/amide ABC transporter ATP-binding protein 1, HAAT family</fullName>
            <ecNumber evidence="6">3.6.3.25</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_0668</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in beta-(1--&gt;2)glucan export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.25" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313990.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0668"/>
    <dbReference type="EnsemblBacteria" id="ABA03934">
        <property type="protein sequence ID" value="ABA03934"/>
        <property type="gene ID" value="Nwi_0668"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0668"/>
    <dbReference type="eggNOG" id="COG0411">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_5"/>
    <dbReference type="OMA" id="IPCFAKR"/>
    <dbReference type="OrthoDB" id="1179151at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="30"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="62"/>
            <end position="69"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA03934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="272" mass="29712" checksum="CE5A3899993755D4" modified="2005-10-11" version="1">MTAVDSSRRQPESRGVLRICAATRSRPPTLELSGIGFSFGGAPVLSGVDLVVAQGERRVILGPNGAGKTTLFNVVCGDYSPKAGSIRFSGRDITDLKQHMRARIGIARTYQNSLLFDGLTVLDNIFLAIRGVRRNRFSLLRPRANDRDLAQAREIADRVRICHLLNSDVRDLSHGQRRQLELGMALAAEPKLLMLDEPAAGLSPGDRPELLRTLHELPRSLTLVVVEHDMDIALPIADIVTVMKDGAVAIERTPDKVERDPAVQAIYLGEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV53</accession>
    <name>Q3SV53_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative methylated-DNA-protein-cystein methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0571</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0571"/>
    <dbReference type="EnsemblBacteria" id="ABA03838">
        <property type="protein sequence ID" value="ABA03838"/>
        <property type="gene ID" value="Nwi_0571"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0571"/>
    <dbReference type="eggNOG" id="COG0350">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2288582_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003908">
        <property type="term" value="F:methylated-DNA-[protein]-cysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036631">
        <property type="entry name" value="MGMT_N_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53155">
        <property type="entry name" value="SSF53155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="101" mass="11587" checksum="F8BD6E50F5C6936A" modified="2005-10-11" version="1">MVWTAHGIEAVQLPLPEEDKTRMRLRQRYRSLAEAAPPAVVRAAIDGVIALLEGKPIDLSGVVLALDSVGEFDRRVYDIARTIPPRQHDDLWRHRQTPWRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV55</accession>
    <name>Q3SV55_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0569</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03836">
        <property type="protein sequence ID" value="ABA03836"/>
        <property type="gene ID" value="Nwi_0569"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0569"/>
    <dbReference type="HOGENOM" id="CLU_2288581_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="101" mass="11286" checksum="AC469264D4625754" modified="2005-10-11" version="1">MVARRGIREPQSRSYAARFHTEVGPARSHLQDGMERATFRAGIPVRCVSIDCASRSGGEFSGVNYIPPKLFLIEPDGNDPSLRETLRGYGPFVQILRNQRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV69</accession>
    <name>Q3SV69_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0555</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313883.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0555"/>
    <dbReference type="EnsemblBacteria" id="ABA03822">
        <property type="protein sequence ID" value="ABA03822"/>
        <property type="gene ID" value="Nwi_0555"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0555"/>
    <dbReference type="eggNOG" id="COG0664">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077340_0_0_5"/>
    <dbReference type="OMA" id="RTAKWIA"/>
    <dbReference type="OrthoDB" id="1596937at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012318">
        <property type="entry name" value="HTH_CRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13545">
        <property type="entry name" value="HTH_Crp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51063">
        <property type="entry name" value="HTH_CRP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH crp-type" evidence="2">
        <location>
            <begin position="152"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="252" mass="27561" checksum="D6E249D02D9A20AD" modified="2005-10-11" version="1">MAIQQDTNRQSRPYNNLLRRLSDADFALIKSSLEVARNEGDDLLYNPGDDVETVHFPCGPSLVSYLVPNEDGRDVETILVGREGAVGGIVSHGHLPAYTRITVKVGGLFVRLRVAKLESAKTKSSTLQNLFARYADCMLAQVFQSTACNAIHSIEQRTAKWILSAMERTGSELVPLTHEQLSTMLGVGRSYASRVIQTFKAEGVLATRRGAIVVCNREALASRACLCNEAVKGHFEEVLRGVYPTDAAQPAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV81</accession>
    <name>Q3SV81_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">UDP-glucose 6-dehydrogenase</fullName>
            <ecNumber evidence="4 7">1.1.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_0543</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>H2O + 2 NAD(+) + UDP-alpha-D-glucose = 3 H(+) + 2 NADH + UDP-alpha-D-glucuronate</text>
            <dbReference type="Rhea" id="RHEA:23596"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="1.1.1.22"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nucleotide-sugar biosynthesis; UDP-alpha-D-glucuronate biosynthesis; UDP-alpha-D-glucuronate from UDP-alpha-D-glucose: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.22" evidence="4 7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313871.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0543"/>
    <dbReference type="EnsemblBacteria" id="ABA03810">
        <property type="protein sequence ID" value="ABA03810"/>
        <property type="gene ID" value="Nwi_0543"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0543"/>
    <dbReference type="eggNOG" id="COG1004">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023810_1_2_5"/>
    <dbReference type="OMA" id="TMDAWSS"/>
    <dbReference type="OrthoDB" id="647136at2"/>
    <dbReference type="UniPathway" id="UPA00038">
        <property type="reaction ID" value="UER00491"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003979">
        <property type="term" value="F:UDP-glucose 6-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006065">
        <property type="term" value="P:UDP-glucuronate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017476">
        <property type="entry name" value="UDP-Glc/GDP-Man"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014027">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036220">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014026">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001732">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028357">
        <property type="entry name" value="UDPglc_DH_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00984">
        <property type="entry name" value="UDPG_MGDP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03720">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03721">
        <property type="entry name" value="UDPG_MGDP_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500134">
        <property type="entry name" value="UDPglc_DH_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000124">
        <property type="entry name" value="UDPglc_GDPman_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00984">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52413">
        <property type="entry name" value="SSF52413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03026">
        <property type="entry name" value="NDP-sugDHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 10" id="KW-0520">NAD</keyword>
    <keyword evidence="5 7 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDPG_MGDP_dh_C" evidence="11">
        <location>
            <begin position="316"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="151"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="251"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="262"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA03810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="433" mass="47325" checksum="FA8F2B86D7DDEC72" modified="2005-10-11" version="1">MRIVMIGAGYVGLVSAACFADFGHDVTCVDKDCEKIAALNQYRIPIFEPDLDKLVESGVNSGRLRFGVDLSDIGEADAVFIAVGTPSRRGDGHADLSYVHAAAREIAGLLEGFTLIVTKSTVPVGTGDEVERIIKETNPEADFAVVANPEFLREGAAIRDFQHPDRIVVGTDDERAKKVMGQIYRPLYLNQAPILYTSRRTAELIKYAANAFLATKITFINEIADLAERVGANVQDVARGIGLDNRIGAKFLHAGPGYGGSCFPKDTRALSKTALDYGVQLRVVEATLTVNEIRKRTMSRKVSGALDNDIRGKTIAVLGLTFKPDTDDMREAPSIALIAGLLDFGAKVRAHDPVGMEQAKKELSDIEFCDDPYACARGADALVIVTEWRQFRALDLERIRTDMATPVIVDLRNIYRPEDVRSAGFRYESIGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVA5</accession>
    <name>Q3SVA5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HPTransfase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0519</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313847.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0519"/>
    <dbReference type="EnsemblBacteria" id="ABA03786">
        <property type="protein sequence ID" value="ABA03786"/>
        <property type="gene ID" value="Nwi_0519"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0519"/>
    <dbReference type="eggNOG" id="COG5385">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086320_0_0_5"/>
    <dbReference type="OMA" id="KATLKWQ"/>
    <dbReference type="OrthoDB" id="1464071at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018762">
        <property type="entry name" value="ChpT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10090">
        <property type="entry name" value="HPTransfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HPTransfase" evidence="1">
        <location>
            <begin position="89"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="215" mass="22477" checksum="31119FC7A7A6EE84" modified="2005-10-11" version="1">MSGTSSPGPAPDALELAALLCSRVCHDLISPVGAIVNGLEVLDDDPKPDDREFALDLIRKSARTASARLQFCRLAFGAAGSVSAQIDLGDAQNIAKGHFEDGKITLAWNLPRLLLPKNRVKLLLNLLVIAQQTIPRGGVLTIDPLGEGEAMGFQITAAGLNARVSQPVAELLASGSTGAVYAHAVQPYYTRLLAEACGISISLVAEGDKVVATAR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVF3</accession>
    <name>HEM3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Porphobilinogen deaminase</fullName>
            <shortName evidence="1">PBG</shortName>
            <ecNumber evidence="1">2.5.1.61</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Hydroxymethylbilane synthase</fullName>
            <shortName evidence="1">HMBS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Pre-uroporphyrinogen synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hemC</name>
        <name type="ordered locus">Nwi_0471</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + 4 porphobilinogen = hydroxymethylbilane + 4 NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13185"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57845"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="EC" id="2.5.1.61"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>dipyrromethane</name>
            <dbReference type="ChEBI" id="CHEBI:60342"/>
        </cofactor>
        <text evidence="1">Binds 1 dipyrromethane group covalently.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">The porphobilinogen subunits are added to the dipyrromethane group.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HMBS family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.61" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313802.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVF3"/>
    <dbReference type="SMR" id="Q3SVF3"/>
    <dbReference type="STRING" id="323098.Nwi_0471"/>
    <dbReference type="EnsemblBacteria" id="ABA03738">
        <property type="protein sequence ID" value="ABA03738"/>
        <property type="gene ID" value="Nwi_0471"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0471"/>
    <dbReference type="eggNOG" id="COG0181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019704_1_2_5"/>
    <dbReference type="OMA" id="LWQANHI"/>
    <dbReference type="OrthoDB" id="1450400at2"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00319"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004418">
        <property type="term" value="F:hydroxymethylbilane synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018160">
        <property type="term" value="P:peptidyl-pyrromethane cofactor linkage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00260">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000860">
        <property type="entry name" value="HemC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022419">
        <property type="entry name" value="Porphobilin_deaminase_cofac_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022417">
        <property type="entry name" value="Porphobilin_deaminase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022418">
        <property type="entry name" value="Porphobilinogen_deaminase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036803">
        <property type="entry name" value="Porphobilinogen_deaminase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557">
        <property type="entry name" value="PTHR11557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01379">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03900">
        <property type="entry name" value="Porphobil_deamC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001438">
        <property type="entry name" value="4pyrrol_synth_OHMeBilane_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00151">
        <property type="entry name" value="PORPHBDMNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54782">
        <property type="entry name" value="SSF54782"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00212">
        <property type="entry name" value="hemC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00533">
        <property type="entry name" value="PORPHOBILINOGEN_DEAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000047755" description="Porphobilinogen deaminase">
        <location>
            <begin position="1"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="modified residue" description="S-(dipyrrolylmethanemethyl)cysteine" evidence="1">
        <location>
            <position position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00260"/>
        </source>
    </evidence>
    <sequence length="316" mass="33985" checksum="D612AFE59E74C8DE" modified="2005-10-11" version="1">MQSSDETDILATIGTRGSPLALAQAHEVRDRLARAHQVAPERIAIKTIRTSGDAIQDRPLFDVGGKGLFTKEIEEALLAGTIDFAVHSSKDVPTFLPDATWLPAFLPREDVRDVFISPHAGSLNDLPAGATVGTASLRRQAMVLKLRPDLKVNSLRGNVETRLRKISVGEADATLLALAGLNRLGLQDKATRILETDEFLPAVGQGAIAIESRRDDDRINAFVKAIGDPETEVALSAERSFLALLDGSCRTPIGGHCRVNGDRIDFRGLIISPDGTEFYETTREGARADAAALGADAAHELRERAGEKFFTLFAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVF7</accession>
    <name>Q3SVF7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Acetyl-coenzyme A synthetase</fullName>
            <shortName evidence="5">AcCoA synthetase</shortName>
            <shortName evidence="5">Acs</shortName>
            <ecNumber evidence="5">6.2.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Acetate--CoA ligase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Acyl-activating enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">acsA</name>
        <name evidence="9" type="ordered locus">Nwi_0467</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>acetate + ATP + CoA = acetyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23176"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="PTM">
        <text evidence="5">Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313798.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0467"/>
    <dbReference type="EnsemblBacteria" id="ABA03734">
        <property type="protein sequence ID" value="ABA03734"/>
        <property type="gene ID" value="Nwi_0467"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0467"/>
    <dbReference type="eggNOG" id="COG0365">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_3_6_5"/>
    <dbReference type="OMA" id="DHWWHDL"/>
    <dbReference type="OrthoDB" id="141801at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019427">
        <property type="term" value="P:acetyl-CoA biosynthetic process from acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01123">
        <property type="entry name" value="Ac_CoA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011904">
        <property type="entry name" value="Ac_CoA_lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02188">
        <property type="entry name" value="Ac_CoA_lig_AcsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 5 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACAS_N" evidence="8">
        <location>
            <begin position="23"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding" evidence="6">
        <location>
            <begin position="82"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="7">
        <location>
            <begin position="529"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Coenzyme A binding" evidence="5">
        <location>
            <begin position="190"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="385"/>
            <end position="387"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="409"/>
            <end position="414"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="498"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="513"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="521"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="524"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="535"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="537"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="540"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="582"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-acetyllysine" evidence="5">
        <location>
            <position position="607"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA03734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="652" mass="72404" checksum="A553C7FEA227DE5F" modified="2005-10-11" version="1">MSDKIYDVSADWARRAWVDDAKYREMYARSIKDPNGFWSEAAQRLDWITPFTKVENTSFELGKVSIKWFEDGVLNAAYNCIDRHLEKRGDQTAIIWEGDDPSQSRHITYRELHDEVCKMANILRLRNVKKGDRVTIYLPMIPEAAYAILACARIGAIHSVVFGGFSPDSIAGRLRDCKSRVIITSDEGLRGGRTIAMKANVDAAIEKVNGEVDWVIVVKRTGGNIDMNPTRDVWYHEAAAVVESECPCEPMNAEDPLFILYTSGSTGQPKGVLHTTAGYLVFVAMTHQYVFDYHDGDIYWCTADVGWVTGHSYILYGPLANGATTMMFEGVPNYPDTSRFWNVIDKHKVNIFYTAPTAIRALMQAGDEPVKKTSRASLRLLGSVGEPINPEAWEWYHRVVGDGRCPVVDTWWQTETGGIMISALPGAIAQKPGSATKPFFGIAPEIVDADGNILEGECSGNLCIARSWPAQVRTVYGDHARFEQTYFSSYKGRYFSGDGCRRDADGYYWITGRVDDVINVSGHRMGTAEVESSLVAHESVSEAAVVGYPHNIKGQGIYAYVTLMKGVEPSESLRKELVAWVRKDIGPIASPDLIQFAPGLPKTRSGKIMRRILRKIAEDEPSSLGDTTTLADPAVVDDLVRNRQNRKDGAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVG5</accession>
    <name>Q3SVG5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Isovaleryl-CoA dehydrogenase</fullName>
            <ecNumber evidence="2">1.3.8.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0459</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.3.8.4" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0459"/>
    <dbReference type="EnsemblBacteria" id="ABA03726">
        <property type="protein sequence ID" value="ABA03726"/>
        <property type="gene ID" value="Nwi_0459"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0459"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063432_0_0_5"/>
    <dbReference type="OMA" id="WRESAFV"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008470">
        <property type="term" value="F:isovaleryl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="1">
        <location>
            <begin position="20"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="367" mass="38826" checksum="6B63631DF6892683" modified="2005-10-11" version="1">MTHVTSDLAASDLEAWLDASASHLDTTTDTAASVLPKLADGGLFRLGVPVQWGGNGGHVADAVNAITETSTRSLTAGFVFWGHRTFIEYLLQSPNAALRERLLPDLLAGRRGGATGLSNAMKFLSGIEELSVQSRRSGTALQLDGYLPWVTNLPATGFDVAMAVKGADGAPAFIAALASDDSGLERSPDLDLMAMRGSNTAALKIESVQIGEDRIIHHHAAEWLPKVRPAFLAMQSALSIGLARRALAEATARLRNDRDILSEPVAALTAELSTAETALLEGLGGGRFIEHPASLFRLRIRLAEIAVQAVQFELSASGGRAYLSQPGAGFQRRLREAAFIPVLTPSLIQLKLALRVQELPAAVGATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVG7</accession>
    <name>Q3SVG7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC-type nitrate/sulfonate/bicarbonate transport systems periplasmic components</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0457</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CmpA/NrtA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313788.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0457"/>
    <dbReference type="EnsemblBacteria" id="ABA03724">
        <property type="protein sequence ID" value="ABA03724"/>
        <property type="gene ID" value="Nwi_0457"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0457"/>
    <dbReference type="eggNOG" id="COG0715">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037398_3_1_5"/>
    <dbReference type="OMA" id="FTGDIWK"/>
    <dbReference type="OrthoDB" id="1832232at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd13553">
        <property type="entry name" value="PBP2_NrtA_CpmA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044527">
        <property type="entry name" value="NrtA/CpmA_ABC-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="400" mass="44147" checksum="0D95E35AE10C7A31" modified="2005-10-11" version="1">MSIDEFSRYDVSRRQMLRLSALLTAGGALPLLKTYEARAREADVPLRIGYLPITDATPLLVAHGKGFFEEAGIKAEKPVRLRSWAQVLEAFISGQVNAVHLLSPMTVWARFGSKAPAKVVAWNHTSGSGLSVLPHINSVRELGGTTVAIPFWYSIHNVVLQILLRENGLKPVSKRTGAPAADEVNLVVMAPADMVPALAAKQISGYIVAEPFNAASEALGVGKILRFTGDVWKDHACCLVFMHERDIQQRPEWVQRAVNAMVKAQLWTREHRAETAALLSKDNPNQYTPHSVEVLSKVLAPPETDDAAYIKAGAIIHPDWRERRIDFQPYPYPSYTEELIRRLRDTLVEGDRAFLDKLDPAAAGRELVDDRFVKKAVIAAGGMAAFDLPESFTRTEVVAA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVI1</accession>
    <name>PROB_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamate 5-kinase</fullName>
            <ecNumber evidence="1">2.7.2.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Gamma-glutamyl kinase</fullName>
            <shortName evidence="1">GK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">proB</name>
        <name type="ordered locus">Nwi_0443</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-glutamate = ADP + L-glutamyl 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:14877"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58274"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutamate 5-kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.11" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313774.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVI1"/>
    <dbReference type="SMR" id="Q3SVI1"/>
    <dbReference type="STRING" id="323098.Nwi_0443"/>
    <dbReference type="EnsemblBacteria" id="ABA03710">
        <property type="protein sequence ID" value="ABA03710"/>
        <property type="gene ID" value="Nwi_0443"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0443"/>
    <dbReference type="eggNOG" id="COG0263">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025400_2_0_5"/>
    <dbReference type="OMA" id="TVIHRDN"/>
    <dbReference type="OrthoDB" id="1480250at2"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00359"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004349">
        <property type="term" value="F:glutamate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04242">
        <property type="entry name" value="AAK_G5K_ProB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00456">
        <property type="entry name" value="ProB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041739">
        <property type="entry name" value="G5K_ProB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001057">
        <property type="entry name" value="Glu/AcGlu_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011529">
        <property type="entry name" value="Glu_5kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005715">
        <property type="entry name" value="Glu_5kinase/COase_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019797">
        <property type="entry name" value="Glutamate_5-kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000729">
        <property type="entry name" value="GK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00474">
        <property type="entry name" value="GLU5KINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01027">
        <property type="entry name" value="proB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00902">
        <property type="entry name" value="GLUTAMATE_5_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0641">Proline biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000230048" description="Glutamate 5-kinase">
        <location>
            <begin position="1"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="PUA" evidence="1">
        <location>
            <begin position="281"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="175"/>
            <end position="176"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00456"/>
        </source>
    </evidence>
    <sequence length="379" mass="39934" checksum="EBB82BADDE85894B" modified="2005-10-11" version="1">MTRPHLKKFRRIVVKVGSSLLIDSAAGKVRGEWLSALAADIAGLHGDGCDVLVVSSGAVALGRSKLKLPRGPLKLEESQAAAAVGQIALARIWSKVLADHGIGAGQILVTWQDTEERRRYLNARSTIAKLLEWRAVPVINENDTVATNEIRYGDNDRLAARVATMASADLLILLSDIDGLYDAPPHLNPDAKLITVVKRVTADIEAMAGSAASELSRGGMRTKIEAAKIATTAGTHMLIASGTIEHPLRAIMDGGPCTWFLTPANPVTARKRWIAGSLEPRGTLAIDAGAVAALRAGKSLLPAGVTRIDGHFARGDAVIVRGPNGHEIGRGLVAYDAADADRIKGRSSSDAAQLLGVRGRVEMIHRDDLVVGGPLGDSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVK7</accession>
    <name>Q3SVK7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA 2',3'-cyclic phosphodiesterase</fullName>
            <shortName evidence="1">RNA 2',3'-CPDase</shortName>
            <ecNumber evidence="1">3.1.4.58</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0417</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Hydrolyzes RNA 2',3'-cyclic phosphodiester to an RNA 2'-phosphomonoester.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + H2O = a 3'-end 2'-phospho-ribonucleotide-RNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11828"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:17353"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:173113"/>
            <dbReference type="EC" id="3.1.4.58"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 2H phosphoesterase superfamily. ThpR family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.58" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313748.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0417"/>
    <dbReference type="EnsemblBacteria" id="ABA03684">
        <property type="protein sequence ID" value="ABA03684"/>
        <property type="gene ID" value="Nwi_0417"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0417"/>
    <dbReference type="eggNOG" id="COG1514">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081251_0_0_5"/>
    <dbReference type="OMA" id="HITLRFL"/>
    <dbReference type="OrthoDB" id="1696971at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004113">
        <property type="term" value="F:2',3'-cyclic-nucleotide 3'-phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008664">
        <property type="term" value="F:2'-5'-RNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01940">
        <property type="entry name" value="RNA_CPDase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009097">
        <property type="entry name" value="Cyclic_Pdiesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004175">
        <property type="entry name" value="RNA_CPDase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35561">
        <property type="entry name" value="PTHR35561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55144">
        <property type="entry name" value="SSF55144"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02258">
        <property type="entry name" value="2_5_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="short sequence motif" description="HXTX 1" evidence="1">
        <location>
            <begin position="37"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="HXTX 2" evidence="1">
        <location>
            <begin position="120"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="37"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="178" mass="19358" checksum="5B765B193B175C31" modified="2005-10-11" version="1">MPRLFTGLEIPPEICRTLSGLRGGLPGARWIDPENYHLTLRFIGDIDGAAANEVASVLLRVNRKPFEVTLRGLSSFGGRKPRAVVASVEPSGPLIELQAELERLMRRCGLGAEGRKFTPHITLARLRDASNQDVANYLSVRGYFPATVFTAERFVLFSSRASVGGGPYVVEDAYALGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="133" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVN1</accession>
    <name>Q3SVN1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Tyrosine recombinase XerD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">xerD</name>
        <name evidence="15" type="ordered locus">Nwi_0393</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules. The XerC-XerD complex is essential to convert dimers of the bacterial chromosome into monomers to permit their segregation at cell division. It also contributes to the segregational stability of plasmids.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Forms a cyclic heterotetrameric complex composed of two molecules of XerC and two molecules of XerD.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the 'phage' integrase family. XerD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313724.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0393"/>
    <dbReference type="EnsemblBacteria" id="ABA03660">
        <property type="protein sequence ID" value="ABA03660"/>
        <property type="gene ID" value="Nwi_0393"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0393"/>
    <dbReference type="eggNOG" id="COG4974">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027562_9_0_5"/>
    <dbReference type="OMA" id="HRFLYAE"/>
    <dbReference type="OrthoDB" id="745068at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009037">
        <property type="term" value="F:tyrosine-based site-specific recombinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01808">
        <property type="entry name" value="Recomb_XerC_XerD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01807">
        <property type="entry name" value="Recomb_XerD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004107">
        <property type="entry name" value="Integrase_SAM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011932">
        <property type="entry name" value="Recomb_XerD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023009">
        <property type="entry name" value="Tyrosine_recombinase_XerC/XerD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02899">
        <property type="entry name" value="Phage_int_SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02225">
        <property type="entry name" value="recomb_XerD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5 11" id="KW-0132">Cell division</keyword>
    <keyword evidence="6 11" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0229">DNA integration</keyword>
    <keyword evidence="9 11" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="8 11 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="14">
        <location>
            <begin position="6"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="13">
        <location>
            <begin position="112"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="186"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="263"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="266"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="289"/>
        </location>
    </feature>
    <feature type="active site" description="O-(3'-phospho-DNA)-tyrosine intermediate" evidence="11">
        <location>
            <position position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABA03660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="319" mass="35379" checksum="642D4265793DD640" modified="2005-10-11" version="1">MKSHNSSDAKLIALFLDMLAAEQGAGENTLDAYRRDLEDLSAFLARARRNFVIAETQTLRDYLNDLDLRGFKSSSVARRLSAMRHLFRFLLSERVRSDDPAAILSGPKRGRSLPKVLSIADVDRLLGRAKADAESLDAPPARRLRAARLYCLLEVLYATGLRVSELVSLPLSAARRDARMIVVRGKGDKERLVPLNNASRQAMADYLQTMADQRGAGKAASPRQSWLFPSFGESGHLTRQHFARELKELAAAAGLAPRLVSPHVLRHAFASHLLHNGADLRIVQTLLGHTDISTTQIYTHVVEERLKSLVRDLHPLAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVT3</accession>
    <name>Q3SVT3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Murein endopeptidase, Metallo peptidase, MEROPS family M74</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0341</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0341"/>
    <dbReference type="EnsemblBacteria" id="ABA03608">
        <property type="protein sequence ID" value="ABA03608"/>
        <property type="gene ID" value="Nwi_0341"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0341"/>
    <dbReference type="eggNOG" id="COG3770">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052496_0_2_5"/>
    <dbReference type="OMA" id="VRPWWGH"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030288">
        <property type="term" value="C:outer membrane-bounded periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009045">
        <property type="entry name" value="Hedgehog_sig/DD-Pept_Zn-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005073">
        <property type="entry name" value="Peptidase_M74"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03411">
        <property type="entry name" value="Peptidase_M74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55166">
        <property type="entry name" value="SSF55166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="350" mass="38323" checksum="C502A83C550BF2B4" modified="2005-10-11" version="1">MQQAAAVMPARRNHHQPLWSRRRENAIESPLMNIRAILPLLLFFGLAAPIGALVPSAVAQDKGTLHPKPLPPLANPDDPALAAKQLFGRKTLPAAGAPYVIGFYARGCIAGAEALPVTGPTWQVMRLSRNRNWAHPAMVALLERVSAKARRDAGWPGLLIGDMAQPRGGPMLTGHASHQIGLDADVWLTPMPNRLLSRNEREEMSAVMMVRRDRLDIDPRAWTPSHLGVIRAAAREPSVERIFVNAAIKKALCREAKGDRSWLSKVRPMYGHDYHFHIRIKCPPGSPQCESQPPPGPAEGCGAQDLAYWFSDAVLHPKPPKTPPKPKPPITLSQLPTACRQVLAAPDARQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVV9</accession>
    <name>Q3SVV9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pseudouridine synthase</fullName>
            <ecNumber evidence="2">4.2.1.70</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0315</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.2.1.70" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313647.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0315"/>
    <dbReference type="EnsemblBacteria" id="ABA03582">
        <property type="protein sequence ID" value="ABA03582"/>
        <property type="gene ID" value="Nwi_0315"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0315"/>
    <dbReference type="eggNOG" id="COG0564">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016902_11_1_5"/>
    <dbReference type="OMA" id="DRHETQF"/>
    <dbReference type="OrthoDB" id="1714699at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140098">
        <property type="term" value="F:catalytic activity, acting on RNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004730">
        <property type="term" value="F:pseudouridylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034470">
        <property type="term" value="P:ncRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd02869">
        <property type="entry name" value="PseudoU_synth_RluA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006224">
        <property type="entry name" value="PsdUridine_synth_RluC/D_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01129">
        <property type="entry name" value="PSI_RLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PseudoU_synth_2" evidence="1">
        <location>
            <begin position="32"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="264" mass="28888" checksum="8026B68DFB8254B2" modified="2005-10-11" version="1">MPQPEIKRTVDTAQPTPEEIQARVLHRDGLMLVIDKPAGIAVHRGPKGGANLEASFDALRFGLPRPPVLAHRLDRDTSGCLVLGRHRKATASLGLLFKHGKISKTYWAVVEGGPSEDEGAIDIPLGRLNEERGWWQKPDPNGLPSTTKWKVLGRSSPDTSTVSGAGVQLTWLSLEPLTGRTHQLRVHCAAMGWPIVGDNIYGTQASLRRSRNEQYVPRLDEPGLHLHARQIAIPLSRNKPPVVVTAPAPAHLHARLRACGWNAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVX8</accession>
    <name>Q3SVX8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Type II secretion system protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_0296</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313629.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0296"/>
    <dbReference type="EnsemblBacteria" id="ABA03563">
        <property type="protein sequence ID" value="ABA03563"/>
        <property type="gene ID" value="Nwi_0296"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0296"/>
    <dbReference type="eggNOG" id="COG2064">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056917_0_0_5"/>
    <dbReference type="OMA" id="SIKRAWP"/>
    <dbReference type="OrthoDB" id="1559784at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="14"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="296"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="T2SSF" evidence="7">
        <location>
            <begin position="185"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA03563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="325" mass="36233" checksum="3405AC95E2FB11B8" modified="2005-10-11" version="1">MLDILIEKFHNPQFMTMVLAAIAASATVYTLITPFFAADGLTKRMKAVASERERIRLRERERLARTEKVSLRQAPKQFISKIVEDLNLGKWVAQEAAREKLVMAGYRGQAPYVTFLFFRMVTPIVMLIGAIAYVFLLSHTQQSTTVKLGICIGATFFGMQAPMLFLHNAITKRQLSIKRAFPDALDLLLICLESGMSIEVAFRKVSTETGNQSVALAEELTLTTAELSYLQDRKVAYENLANRTGVEGVKSVCLALQQSERYGTPLAQSLRVMAQENRDMRMTEAEKKAAALPPKLTVPMIVFFLPVLFVVILGPTGIKIAAQMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVX9</accession>
    <name>Q3SVX9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TPR repeat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0295</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0295"/>
    <dbReference type="EnsemblBacteria" id="ABA03562">
        <property type="protein sequence ID" value="ABA03562"/>
        <property type="gene ID" value="Nwi_0295"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0295"/>
    <dbReference type="eggNOG" id="COG5010">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072295_0_0_5"/>
    <dbReference type="OMA" id="AHSPDNP"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014596">
        <property type="entry name" value="UCP035836"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF035836">
        <property type="entry name" value="UCP035836"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229271" evidence="3">
        <location>
            <begin position="23"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="77"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="145"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="261"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="268"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="290" mass="31186" checksum="31EECB6802BB74DE" modified="2005-10-11" version="1">MKSTMRRQSCLARLLASSTVTAMLAIGLGGCQTAAISDVTGSLGAKAETKSEKHSGTDPHRTVEVYGERFRANPKDADAALRYGLALRAIGQRAQAVAVLERAAILNPGNKVVLAGWGRALAENGQSQQAFDVLSRAHTPASPDWRILSVQGTTLDKLGRHDDARRYYASALRIMPDEPSVLSNLGMSYVLTKELPKAEETLRRAHAGAPVDSRIRQNLALAVGLQGRFDEAENIVKADLPIDEAAANVAYLKQMLGRGGDARVGAADNVRRNSRRSRADSDPRPDRPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVZ0</accession>
    <name>Q3SVZ0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Methylenetetrahydrofolate reductase</fullName>
            <ecNumber evidence="10">1.5.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_0284</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2 10">
            <text>(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NAD(+) = (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:19821"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:18608"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.5.1.20"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NADP(+) = (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:19817"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:18608"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3 10">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 10">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 10">Belongs to the methylenetetrahydrofolate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.20" evidence="10"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0284"/>
    <dbReference type="EnsemblBacteria" id="ABA03551">
        <property type="protein sequence ID" value="ABA03551"/>
        <property type="gene ID" value="Nwi_0284"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0284"/>
    <dbReference type="eggNOG" id="COG0685">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025841_0_0_5"/>
    <dbReference type="OMA" id="EMHPQAR"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106312">
        <property type="term" value="F:methylenetetrahydrofolate reductase NADH activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106313">
        <property type="term" value="F:methylenetetrahydrofolate reductase NADPH activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00537">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003171">
        <property type="entry name" value="Mehydrof_redctse-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004620">
        <property type="entry name" value="MTHF_reductase_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02219">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="SSF51730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00676">
        <property type="entry name" value="fadh2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0274">FAD</keyword>
    <keyword evidence="6 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="8 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA03551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="343" mass="37414" checksum="CD9B2884051C57E7" modified="2005-10-11" version="1">MGYAGGNAVPRMAGPTPGVSRPTAVTRARRDRCSGVFRAMTDAFAPAHDGLGARKRPTISFEFFPPKTEEMERNLWGTITRLAPLDPSFVSVTYGAGGSTRERTHSTIARILSDTRLRPAAHLTCVGAPRGEIDEIVSRYRDIGVRHIVALRGDPPGGIGTAYSTHPEGYRTSADLVAGIKRRHGDIEISVSAYPEKHPESPDIDTDIDVLKAKVDAGATRAITQVFFDNNLYLRYLDRVRARGIDIPIVPGIMPMHNFKQARAFVTKAGTTVPAWLAEKFEGLDEDPETRKLIAATVAAGQVQKLAKRGVDTFHFYTMNRADLVFAICHLLGIRPDAAQKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVZ3</accession>
    <name>Q3SVZ3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycerol kinase</fullName>
            <ecNumber evidence="5">2.7.1.30</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">ATP:glycerol 3-phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Glycerokinase</fullName>
            <shortName evidence="5">GK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">glpK</name>
        <name evidence="9" type="ordered locus">Nwi_0281</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + glycerol = ADP + H(+) + sn-glycerol 3-phosphate</text>
            <dbReference type="Rhea" id="RHEA:21644"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.30"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="5">Inhibited by fructose 1,6-bisphosphate (FBP).</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the FGGY kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.30" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0281"/>
    <dbReference type="EnsemblBacteria" id="ABA03548">
        <property type="protein sequence ID" value="ABA03548"/>
        <property type="gene ID" value="Nwi_0281"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0281"/>
    <dbReference type="eggNOG" id="COG0554">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009281_2_3_5"/>
    <dbReference type="OMA" id="FMLMNIG"/>
    <dbReference type="UniPathway" id="UPA00618">
        <property type="reaction ID" value="UER00672"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004370">
        <property type="term" value="F:glycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006072">
        <property type="term" value="P:glycerol-3-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00186">
        <property type="entry name" value="Glycerol_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000577">
        <property type="entry name" value="Carb_kinase_FGGY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="Carb_kinase_FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018483">
        <property type="entry name" value="Carb_kinase_FGGY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="Carb_kinase_FGGY_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005999">
        <property type="entry name" value="Glycerol_kin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000538">
        <property type="entry name" value="GlpK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01311">
        <property type="entry name" value="glycerol_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00445">
        <property type="entry name" value="FGGY_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="5 6 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FGGY_N" evidence="7">
        <location>
            <begin position="24"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="FGGY_C" evidence="8">
        <location>
            <begin position="279"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="101"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="262"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="31"/>
            <end position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="431"/>
            <end position="435"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA03548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="519" mass="56103" checksum="27F342D60E20EEF9" modified="2005-10-11" version="1">MPLWRVAPPPRARKFENNVMVTHVLAIDQGTTSSRAIVFRADLTVAAIAQQEFPQHFPASGWVEHEPEDIWTSTVATCRQAMKKAGVTPKDIAAIGITNQRETTVVWDRVTGKAVRRAIVWQDRRTADVCARLKAEGHEPAISAKTGLIIDPYFSGTKVAWILDSVPGARARAMRGELMFGTVDCYLLWRLTGGKVHATDATNASRTMLFDIHTGHWDDALLDILRIPRAMLPEVKDSSAHFGDSAPELFGAPIAIRGIAGDQQAAVVGQACFTPGMIKSTYGTGCFALLNTGATPVASTNKLLTTIAYQLNGERTYALEGSIFVAGSAVQWLRDGLGLIKNASETEPLAEQSDPVQSVYLVPAFVGMGAPYWNPNVRGALFGLTRNTGPAELAHAALESVCYQTFDLWEAMRADWPADAAAHTVLGVDGGMTASNWTMQRLADLLGVPVDRPVIQETTALGAAYLAGLSAGVYPEPSRFADNWRRDRCFKPAMSAETRARKLKGWTAAVRGVLASDEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW04</accession>
    <name>Q3SW04_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DEAD/DEAH box helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0270</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313603.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0270"/>
    <dbReference type="REBASE" id="11315">
        <property type="entry name" value="NwiORF269P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03537">
        <property type="protein sequence ID" value="ABA03537"/>
        <property type="gene ID" value="Nwi_0270"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0270"/>
    <dbReference type="eggNOG" id="COG4096">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018958_0_0_5"/>
    <dbReference type="OMA" id="DGFIFHD"/>
    <dbReference type="OrthoDB" id="16977at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006304">
        <property type="term" value="P:DNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013670">
        <property type="entry name" value="EcoEI_R_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08463">
        <property type="entry name" value="EcoEI_R_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="2">
        <location>
            <begin position="171"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="560"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="563"/>
            <end position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="790" mass="89636" checksum="A02FD51C54995867" modified="2005-10-11" version="1">MTNEADTCRKFVVPKLQSCGWDNEPHSIAEQRSITDGRVIPVGKGFVRKPPKRVDYLLRYTRDFPLGVVEAKAGYKLATDAVQQARDYAETLGLKFAYATNGIEIIEIDYFAGTEKRVADFPKPEELWRRYQAGSGMDTPERVDHLIAPYNTVGGKPPRYYQQIAINRTVESILAGKKRLLLTMATGTGKTIVAFQVCWKLWSSRWNKTGEHRKPRILFLADRNILIDDPKDKTFTPFGDARHKIESGEIIKSREMYFAIYQALAEDERRAGLFRDYPPDFFDLIIVDECHRGSARDDSSWRVILEHFKPAYQLGMTATPLRQDNRDTYLYFGNPIYEYSLRQGIDDGFLAPYRVHRVITQWDAAGWRPSKDEVDRYGRAIPDDEYQTKDFERVIALRARTQAIARHLTDFLRKTDRFAKTIVFCVDQEHASEMREALVNLNSDLIAQYPDYVARVTADEGTIGRGHLSRFQDVETKTPVILTSSQLLTTGVDAPTCKNVVLARVVGSMSEFKQIIGRGTRLRDDYGKLWFNILDYTGAATRMFADPDFDGDPARITEEEVNDAGEATSTTETIPEGQEAEPATPEEGEPGVIEPPTGEPRKFYFDGGQVEVVAHLVHELDPNGKQLRVVKYTEYAAESIRSLAPTSADLRQRWADADQRREIIAALAERGIDFAVLAEQTGQTDADPFDLLCHLAFNAPLRTRRERAQRLKSERKDYFEKFSPEARQVLDDLLEKYAEHGDAQFVLPDVLKVPPISTHGQPAEIIKLFGGPDELRRAVNDLQGLLYGTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWB6</accession>
    <name>Q3SWB6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Heparinase II/III-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0157</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313494.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0157"/>
    <dbReference type="EnsemblBacteria" id="ABA03425">
        <property type="protein sequence ID" value="ABA03425"/>
        <property type="gene ID" value="Nwi_0157"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0157"/>
    <dbReference type="eggNOG" id="COG5360">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025266_0_0_5"/>
    <dbReference type="OMA" id="IDRMMPM"/>
    <dbReference type="OrthoDB" id="442785at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008929">
        <property type="entry name" value="Chondroitin_lyas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012480">
        <property type="entry name" value="Hepar_II_III"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07940">
        <property type="entry name" value="Hepar_II_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="584" mass="64476" checksum="AE6496A732D47942" modified="2005-10-11" version="1">MSVAHRRRISALVVSRFARRTMARAGDGPAALSRLWPGRADRLTIAPHDLRTADATRAAEIYAGRFVFAGKIVTCHSRSVFDLEPPSEDWEAALLGFGWLRHLRAADTAITRANARSLVEDWISNPARRRPACRRADVMSRRVISLLSQAPLVLGEADGKFYRRYLRALARDIRLLRFGLHDAPDGVSRLQVVIALCYASLCLANQARTIRSATRKLSEELRRQILPDGGHVSRNPGALIELLIDLLPLRQTFAARNIAPPPALLNAIDRMMPMLRFFRHGDGSFALFNGMSSTPSDLLATLLAYDDTRGIPMASMPHTGFQRIDAGATILIVDAGLPPPPNLSEEAHAGCLSFELSSGLNRIVTNCGMSSTGRDTWRVFARSTAAHSTLVCHQTSSCQFVEQSAMKRLLQGAPIVSGPAHVESLRETIDGGERLTASHDGYLARFGLIHRRVLTMAHDGSRLEGEDMLEPAPGGRIKGSQIRYALRFHLHPSVKATRLDDAHGVMLVLPNREVWTFEATQDRVELEDSVFLAGNDGPRRTVQIVIHQNAREVSRVRWGFVRSATSAAATNARRQARREPELPL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-17" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWD2</accession>
    <name>CRCB_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative fluoride ion transporter CrcB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">crcB</name>
        <name type="ordered locus">Nwi_0141</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Important for reducing fluoride concentration in the cell, thus reducing its toxicity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CrcB (TC 9.B.71) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313478.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWD2"/>
    <dbReference type="SMR" id="Q3SWD2"/>
    <dbReference type="EnsemblBacteria" id="ABA03409">
        <property type="protein sequence ID" value="ABA03409"/>
        <property type="gene ID" value="Nwi_0141"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0141"/>
    <dbReference type="eggNOG" id="COG0239">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114342_2_3_5"/>
    <dbReference type="OMA" id="EWRLFLI"/>
    <dbReference type="OrthoDB" id="1942863at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903425">
        <property type="term" value="F:fluoride transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00454">
        <property type="entry name" value="CrcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003691">
        <property type="entry name" value="CrcB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28259">
        <property type="entry name" value="PTHR28259"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02537">
        <property type="entry name" value="CRCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00494">
        <property type="entry name" value="crcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000252903" description="Putative fluoride ion transporter CrcB">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00454"/>
        </source>
    </evidence>
    <sequence length="126" mass="13327" checksum="886A03D5868206B4" modified="2005-10-11" version="1">MKWTFILAVAAGGALGSVARYLVGIGFGKWLGPKFPWGTLFINVTGSLLIGIFAGLFAVRWSLPQAARIFLVVGICGGYTTFSTFSLDTFYLIERGEMASAAAYMIGSVVLSVGALIAGIQIVRVI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-09-02" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWH5</accession>
    <name>MNME_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA modification GTPase MnmE</fullName>
            <ecNumber evidence="1">3.6.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mnmE</name>
        <name evidence="1" type="synonym">trmE</name>
        <name type="ordered locus">Nwi_0098</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="1">Binds 1 potassium ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Heterotetramer of two MnmE and two MnmG subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.-.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313435.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWH5"/>
    <dbReference type="SMR" id="Q3SWH5"/>
    <dbReference type="STRING" id="323098.Nwi_0098"/>
    <dbReference type="EnsemblBacteria" id="ABA03366">
        <property type="protein sequence ID" value="ABA03366"/>
        <property type="gene ID" value="Nwi_0098"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0098"/>
    <dbReference type="eggNOG" id="COG0486">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019624_3_1_5"/>
    <dbReference type="OMA" id="CEIQCHG"/>
    <dbReference type="OrthoDB" id="263682at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04164">
        <property type="entry name" value="trmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00379">
        <property type="entry name" value="GTPase_MnmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031168">
        <property type="entry name" value="G_TrmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018948">
        <property type="entry name" value="GTP-bd_TrmE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004520">
        <property type="entry name" value="GTPase_MnmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027368">
        <property type="entry name" value="MnmE_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025867">
        <property type="entry name" value="MnmE_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT_dom1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12631">
        <property type="entry name" value="MnmE_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10396">
        <property type="entry name" value="TrmE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00450">
        <property type="entry name" value="mnmE_trmE_thdF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51709">
        <property type="entry name" value="G_TRME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0630">Potassium</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000345853" description="tRNA modification GTPase MnmE">
        <location>
            <begin position="1"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="TrmE-type G">
        <location>
            <begin position="218"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>(6S)-5-formyl-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>(6S)-5-formyl-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>(6S)-5-formyl-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="228"/>
            <end position="233"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="247"/>
            <end position="253"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="272"/>
            <end position="275"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="456"/>
        </location>
        <ligand>
            <name>(6S)-5-formyl-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00379"/>
        </source>
    </evidence>
    <sequence length="456" mass="49258" checksum="3E28CDB65513B938" modified="2005-10-11" version="1">MHPREQTIFALSSGRPPSAIAVVRASGSQAGTALRKLAGAIPAARTAKRVLLRDSVGEAIDDAVILWFPAPASATGEDIAEFHVHGSRAVVNALIAALSSIDDARAAEPGEFTRRAFENGKIDLTEAEGLDDLIHADTDRQRRQALRQLQGLLGDRARGWRKELIDASALIEAGIDFADEEDVPEDLARPALEKIAQLRREIEQTLAASARGERLRDGLTVAIAGPPNAGKSTLLNRLARREAAIVSPHAGTTRDIIEVYLDLDGYPVTLIDTAGIRETDDPVEQEGVRRARDRVAKADLVLWLSDDHGGEPDQTIRSGETSLWLIRNKIDLAGPENDGAKPGRFETKAGQSAPPWRFRISASRGDGVDDLVSALTAFAEKLLGSSEAGVITRLRHRNILRDAVASLAKAETLAGRGDELVAEELRIAIHLMGRLLGRVDIDDILDSLFKEFCIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWJ4</accession>
    <name>Q3SWJ4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase</fullName>
            <ecNumber evidence="9">1.14.13.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_0079</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UbiH/COQ6 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.13.-" evidence="9"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313416.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0079"/>
    <dbReference type="EnsemblBacteria" id="ABA03347">
        <property type="protein sequence ID" value="ABA03347"/>
        <property type="gene ID" value="Nwi_0079"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0079"/>
    <dbReference type="eggNOG" id="COG0654">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009665_8_1_5"/>
    <dbReference type="OMA" id="AHGFNFG"/>
    <dbReference type="OrthoDB" id="867226at2"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016709">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018168">
        <property type="entry name" value="Ubi_Hdrlase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010971">
        <property type="entry name" value="UbiH/COQ6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01988">
        <property type="entry name" value="Ubi-OHases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01304">
        <property type="entry name" value="UBIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_3" evidence="8">
        <location>
            <begin position="7"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA03347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="406" mass="43662" checksum="A7FADC38222E7E30" modified="2005-10-11" version="1">MTTRQSIVICGGAFAGLALALALRQGLGNEIPIVVADPMLANRPSRDPRATAIVAACRRLFEALGVWDEVAATAQPMLDMVVTDSKLEDATRPVFLTFAGDVQPGEPFAHMVENRYLIEALASRAETAGVDLRATAVTGNDAKPGGVTVSLADGSTIEASLLVAADGARSKLRERAGIATHGWEYDQSGIVVTVGHERDHQGRAEEHFLPAGPFAILPLTGRRSSLVWTEKRADAARIVALAEEEFHAELEQRFGLHLGEVRALDKPRAFPLQYFVARSFIAERLALVGDAAHVIHPIAGQGLNLGLKDVAALAEVVVDAVRLGIDPGQADVLERYQRWRRFDTMAMGLATNTLNVLFSNESTLLRGVRDIGLGLVDRMPPLKNAFIRQAAGLTGEVPRLLKGEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWL0</accession>
    <name>Q3SWL0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Amine oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0063</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313402.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0063"/>
    <dbReference type="EnsemblBacteria" id="ABA03331">
        <property type="protein sequence ID" value="ABA03331"/>
        <property type="gene ID" value="Nwi_0063"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0063"/>
    <dbReference type="eggNOG" id="COG1231">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004498_10_3_5"/>
    <dbReference type="OMA" id="GGQWIGH"/>
    <dbReference type="OrthoDB" id="1442136at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amino_oxidase" evidence="2">
        <location>
            <begin position="43"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Amino_oxidase" evidence="2">
        <location>
            <begin position="199"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="433"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="433"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="480" mass="50842" checksum="62E5CF62EAD40B9F" modified="2005-10-11" version="1">MRITRRDFLSASAGCAAGMSIGRARAAPLPRDPDVIVIGAGAAGIAAARRVQAANRTVLVLEAAGRIGGRCHTDTTTFGTPFDRGARWLFEPDTNPIIRLARSAGMEVFPSPLGQKIRIGRRNARAGETEQFLAALMRAKRAIGEAARRKVDVACASMLPEDLDVWAETIDFVLGASATGKDLKDLSVMDRASAPERNVAIDCRQGLGVLLATLGQAVPVSLSTPVTRVTWTGRSLTVETSAGRIDTRAVIVTVSVNVLTSGHIRFTPELPKRQLDAAARLSLGSYDRIALWLPDNPLGLGPGETMIEQSSDGKTALLVANAHGSSLCTVDIAGGFGRDLSAQGTSAMTAFATEWLTKLFGSDAADAVKQSATTRWNADPHVLGAMSAAEPGGHPSRKILMEPLGNLFLAGEAAHETLWGTVNGAWESGERAADAALRKIGASREKEPAQPQASKHKQPRAQRRTPSRRGNDAFGFPLPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWL4</accession>
    <name>Q3SWL4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Signal peptide peptidase A, Serine peptidase, MEROPS family S49</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0059</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S49 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0059"/>
    <dbReference type="EnsemblBacteria" id="ABA03327">
        <property type="protein sequence ID" value="ABA03327"/>
        <property type="gene ID" value="Nwi_0059"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0059"/>
    <dbReference type="eggNOG" id="COG0616">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046540_0_0_5"/>
    <dbReference type="OMA" id="NCKEYLL"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07023">
        <property type="entry name" value="S49_Sppa_N_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004635">
        <property type="entry name" value="Pept_S49_SppA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002142">
        <property type="entry name" value="Peptidase_S49"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01343">
        <property type="entry name" value="Peptidase_S49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00706">
        <property type="entry name" value="SppA_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_S49" evidence="2">
        <location>
            <begin position="109"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="327" mass="35035" checksum="1DA182C20C96209F" modified="2005-10-11" version="1">MSMSMESDMIVDRRRLRRKLTFWRVASALIVIGAVVALGAYASPLGGRLTGSSAIQRVNIDGLIRSDQKRVEALERLGKSKAPAVIVHINSPGGTTAGSEELYEALTRLKAKKPMVVVVEGLGASGGYIAALASDHIVAQQTALVGSIGVLFQYPNVTELLKTVGVKVEAVKSSPLKAAPSGYEPTSPEARAAIDALVKDSFAWFRGLVQNRRGMDDAQLEKVADGRVFTGRQALDLKLIDQLGDEKVAIAWLVAEKKVAPDLPVRDFKLTPRFTDLTFIRTAASVAFDALGLGSIARQVEQAGIVRAADRLNLDGMLALWHPAPSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="127" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWM2</accession>
    <name>Q3SWM2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thioredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0051</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the thioredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313390.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0051"/>
    <dbReference type="EnsemblBacteria" id="ABA03319">
        <property type="protein sequence ID" value="ABA03319"/>
        <property type="gene ID" value="Nwi_0051"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0051"/>
    <dbReference type="eggNOG" id="COG3118">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090389_10_2_5"/>
    <dbReference type="OMA" id="QVGVAPK"/>
    <dbReference type="OrthoDB" id="1630944at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005746">
        <property type="entry name" value="Thioredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000077">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01068">
        <property type="entry name" value="thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3 5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="6">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="5">
        <location>
            <begin position="31"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000077-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="106" mass="11427" checksum="BB335A36D3BBB90E" modified="2005-10-11" version="1">MAVGKVSDANFEAEVLKATGPVVVDFWAEWCGPCRMIGPVLDEISSAMGSKVKIVKLNVDESPRTASKYGVMSIPTLMIFKDGELASRQVGAAPKQKLEQWITATV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-04-29" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWP5</accession>
    <name>PNP_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Polyribonucleotide nucleotidyltransferase</fullName>
            <ecNumber evidence="1">2.7.7.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Polynucleotide phosphorylase</fullName>
            <shortName evidence="1">PNPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pnp</name>
        <name type="ordered locus">Nwi_0028</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>phosphate + RNA(n+1) = a ribonucleoside 5'-diphosphate + RNA(n)</text>
            <dbReference type="Rhea" id="RHEA:22096"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the polyribonucleotide nucleotidyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.8" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313367.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWP5"/>
    <dbReference type="SMR" id="Q3SWP5"/>
    <dbReference type="STRING" id="323098.Nwi_0028"/>
    <dbReference type="EnsemblBacteria" id="ABA03296">
        <property type="protein sequence ID" value="ABA03296"/>
        <property type="gene ID" value="Nwi_0028"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0028"/>
    <dbReference type="eggNOG" id="COG1185">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004217_2_2_5"/>
    <dbReference type="OMA" id="LHILDVM"/>
    <dbReference type="OrthoDB" id="122725at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004654">
        <property type="term" value="F:polyribonucleotide nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01595">
        <property type="entry name" value="PNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012162">
        <property type="entry name" value="PNPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015848">
        <property type="entry name" value="PNPase_PH_RNA-bd_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036456">
        <property type="entry name" value="PNPase_PH_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11252">
        <property type="entry name" value="PTHR11252"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03726">
        <property type="entry name" value="PNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005499">
        <property type="entry name" value="PNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46915">
        <property type="entry name" value="SSF46915"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="SSF54791"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="SSF55666"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03591">
        <property type="entry name" value="polynuc_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000329735" description="Polyribonucleotide nucleotidyltransferase">
        <location>
            <begin position="1"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="domain" description="KH" evidence="1">
        <location>
            <begin position="554"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="1">
        <location>
            <begin position="623"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="692"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="487"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="493"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="718" mass="78044" checksum="32A8C89DC2E962A5" modified="2005-10-11" version="1">MFNQHSVEIDWGGRPLKLETGKIARQADGAVVATYGETVVLATVVAAKSPREGVDFLPLTVDYQEKTYAAGRIPGGYFKREGRPTEKETLVSRLIDRPVRPLFADGWRNETQVIVTVLSHDMENDPDILAMVAASAALTLSGAPFKGPIGAARVGFINDEYVLNPTLDEIVDTQLDLVVAGTADAVLMVESEAKELSEEIMLKAVMFGHRHFQPVIDAIIELAEKAAKEPRELKVIDDTEIEKEMLGLVEQDLRAAYAIPVKQDRYAAVGKVKEKALAHFFPEGQEPKYDKLRVAGVFKELEAKIVRWNILDTGKRIDGRDVKTVRNIVAEAGVLPRAHGSALFTRGETQAMVVTTLGTGEDEQYIDSLAGTYKETFLLHYNFPPYSVGETGRLGGAKRREIGHGKLAWRAIRPVLPPHHEFPYTIRVVSEITESNGSSSMASVCGASLSLMDAGVPLKRPTAGIAMGLILEGERYAVLSDILGDEDHLGDMDFKVAGTEAGITSLQMDIKIAGITEEIMKVALGQAKDGRIHILGEMSKALDRARAELGEHAPRIETFKIPTDKIREVIGTGGKVIREIVEKTGAKVNIEDDGTVKVASSDGESIKAAIKWIKSIASDPEVGEIYEGTVVKVMEFGAFVNFFGAKDGLVHISQLAAGRVQKTSDVVKEGAKVKVKLLGFDDRGKTRLSMKVVDQETGEDLEAKQKAEGDAPREAAGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMV9</accession>
    <name>Q3SMV9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phage integrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_3135</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316290.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06382">
        <property type="protein sequence ID" value="ABA06382"/>
        <property type="gene ID" value="Nwi_3135"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3135"/>
    <dbReference type="eggNOG" id="COG0582">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063183_0_0_5"/>
    <dbReference type="OMA" id="HPIRLKN"/>
    <dbReference type="OrthoDB" id="1729055at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyr recombinase" evidence="2">
        <location>
            <begin position="153"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="359" mass="40163" checksum="46AFB19C13C28AC3" modified="2005-10-11" version="1">MMPATSKVPASRPHRFQARSLPIAEWPAADRDAWIAACRPSQRLVRGGAGAHMKPITLSDLARRYGYFLDFLDRSGLFDRRRPAAGNVTPENVAGYLNELQTRVSSVTVYGSIYKLRRASELLDPHSEFFWLTEIEKDLALMMRPRSKADRFVLSEVLVENGLTLMAEADHSTTCSDLAKARQFRNGLMVAMLALHPIRLKNFAGLEIGRNFVNIEGRWWIILGAAETKEKRPDERRLDDMLAPMLEKYLSNYLDVLAQESESNALWLSSADGHPMTYSAVADAIKRTTSTAIGVGVSSHMFRTAAASSAAVHAGHNPYLASALLHHRDPRVTEEHYNRASSLSAAKKFARIIRRDRDC</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN28</accession>
    <name>FMT_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methionyl-tRNA formyltransferase</fullName>
            <ecNumber evidence="1">2.1.2.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fmt</name>
        <name type="ordered locus">Nwi_3065</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Attaches a formyl group to the free amino group of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6S)-10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = (6S)-5,6,7,8-tetrahydrofolate + H(+) + N-formyl-L-methionyl-tRNA(fMet)</text>
            <dbReference type="Rhea" id="RHEA:24380"/>
            <dbReference type="Rhea" id="RHEA-COMP:9952"/>
            <dbReference type="Rhea" id="RHEA-COMP:9953"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:78844"/>
            <dbReference type="EC" id="2.1.2.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Fmt family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.9" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316229.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SN28"/>
    <dbReference type="SMR" id="Q3SN28"/>
    <dbReference type="STRING" id="323098.Nwi_3065"/>
    <dbReference type="EnsemblBacteria" id="ABA06313">
        <property type="protein sequence ID" value="ABA06313"/>
        <property type="gene ID" value="Nwi_3065"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3065"/>
    <dbReference type="eggNOG" id="COG0223">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033347_1_2_5"/>
    <dbReference type="OMA" id="CCPVVAY"/>
    <dbReference type="OrthoDB" id="2009156at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004479">
        <property type="term" value="F:methionyl-tRNA formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08646">
        <property type="entry name" value="FMT_core_Met-tRNA-FMT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08704">
        <property type="entry name" value="Met_tRNA_FMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00182">
        <property type="entry name" value="Formyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005794">
        <property type="entry name" value="Fmt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005793">
        <property type="entry name" value="Formyl_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037022">
        <property type="entry name" value="Formyl_trans_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011034">
        <property type="entry name" value="Formyl_transferase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044135">
        <property type="entry name" value="Met-tRNA-FMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041711">
        <property type="entry name" value="Met-tRNA-FMT_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02911">
        <property type="entry name" value="Formyl_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50486">
        <property type="entry name" value="SSF50486"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="SSF53328"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00460">
        <property type="entry name" value="fmt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000020112" description="Methionyl-tRNA formyltransferase">
        <location>
            <begin position="1"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="111"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>(6S)-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00182"/>
        </source>
    </evidence>
    <sequence length="314" mass="33697" checksum="76C15B0835A71494" modified="2005-10-11" version="1">MPLRLIFMGTPDFAVPTLQQLVAHGHEIAAVYTREARPAGRGMKLQPTPVAREAQRLGLPVLTPKTLKTQESQDQLRSYGADAAVVVAYGLILPQAILDAPRYGCYNLHASLLPRWRGAAPINRAVMAGDAESGVMVMKIDAGLDTGDVAMAERVPITDAMTASDLHDTLAPLGADLMARAMDALERGELRLTKQSEQGVASTVTYAAKIDKAEAHIVWSNPAREVLRHIHGLSPFPGAWCEMPVDGEAVRIKILRCELAEGAGSPGDLLDDRLTIACKDGAIRVLELQRAGKPPMKAAAFLNGTPLTPPLRLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN90</accession>
    <name>Q3SN90_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">2-nitropropane dioxygenase</fullName>
            <ecNumber evidence="2">1.13.12.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_3001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.13.12.16" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316173.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3001"/>
    <dbReference type="EnsemblBacteria" id="ABA06251">
        <property type="protein sequence ID" value="ABA06251"/>
        <property type="gene ID" value="Nwi_3001"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3001"/>
    <dbReference type="eggNOG" id="COG2070">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038732_1_0_5"/>
    <dbReference type="OMA" id="DDIPNMI"/>
    <dbReference type="OrthoDB" id="275153at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018580">
        <property type="term" value="F:nitronate monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd04730">
        <property type="entry name" value="NPD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004136">
        <property type="entry name" value="NMO"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03060">
        <property type="entry name" value="NMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NMO" evidence="1">
        <location>
            <begin position="3"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="328" mass="35266" checksum="940E1B323E0B98EA" modified="2005-10-11" version="1">MKTAITELFGIEHPIIQGGMHYVGFAELAAAVSNAGGLGIITGLTQKTPELLAKEIARCQDMTGRPFGVNLTFLPAFTAPPYPEYIRAIVESGVRIVETAGRNPEAYMAALKAAGVKVIHKCTSVRHALKAEQIGCDAVSVDGFECGGHPGEDDIPNMILLPRAADELRIPFVASGGMADARSLVAALALGAEGMNMGTRFIATKEAPVHDNVKQALVAASERDTRLIMRALRNTERVLNNRKVERLMEIEREKGDSLRIDDIMEQVAGVYPRVMIEGDVNAGAWSCGMTAGLIHDVPSCKDLIERIVSGAERIIRQRLTGLLEGRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN91</accession>
    <name>Q3SN91_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_3000</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316172.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3000"/>
    <dbReference type="EnsemblBacteria" id="ABA06250">
        <property type="protein sequence ID" value="ABA06250"/>
        <property type="gene ID" value="Nwi_3000"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3000"/>
    <dbReference type="eggNOG" id="COG4949">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035873_0_0_5"/>
    <dbReference type="OMA" id="HSEFTTY"/>
    <dbReference type="OrthoDB" id="427147at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021830">
        <property type="entry name" value="DUF3422"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11902">
        <property type="entry name" value="DUF3422"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="368"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="407"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="438" mass="48043" checksum="BFBD0757E46977D6" modified="2005-10-11" version="1">MSAEVSIGDAEAARLAPHPLRNAILGELHARPFTALTVPARILHFAFDTAGERGHADRVNLAKFCAARGLPVPTFSERHYRVAFGTTALRWEQHSEFTTYTWELPSPANGIPFHPPAAALATPMQLVPQPGPLLVAIDMHVLADGPAVQPPERLFERTSLALAENNDGAAIYATDFQPDPSGFVRILVVDRKLNPDRAGALAQRVIEIETYRTLALLGLPEAQRLAPSISAVEQRLAEVTDKMRNNGNLADNRHLLDELTALAAEVEAGAAASVFRFGASRAYYEIVQQRLATVGERKVAGLPTWTSFLARRMAPAMRTCVTTEARQESLSLKLSRTANLLRTRVDVELEQQNQELLKSMNARTRMQLRLQTTVEGLSVAAITYYVVSLFGYIAKALHESGTLAIKPGHLTAAFIPVAAFTIWSIVRRIRHKHIGHDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN96</accession>
    <name>Q3SN96_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Acyl-coenzyme A dehydrogenase</fullName>
            <ecNumber evidence="6">1.3.8.7</ecNumber>
            <ecNumber evidence="7">1.3.8.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">Nwi_2995</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a long-chain 2,3-saturated fatty acyl-CoA + H(+) + oxidized [electron-transfer flavoprotein] = a long-chain (2E)-enoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:17721"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:83721"/>
            <dbReference type="ChEBI" id="CHEBI:83727"/>
            <dbReference type="EC" id="1.3.8.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a medium-chain 2,3-saturated fatty acyl-CoA + H(+) + oxidized [electron-transfer flavoprotein] = a medium-chain (2E)-enoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:14477"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:83723"/>
            <dbReference type="ChEBI" id="CHEBI:83726"/>
            <dbReference type="EC" id="1.3.8.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.7" evidence="6"/>
    <dbReference type="EC" id="1.3.8.8" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2995"/>
    <dbReference type="EnsemblBacteria" id="ABA06245">
        <property type="protein sequence ID" value="ABA06245"/>
        <property type="gene ID" value="Nwi_2995"/>
    </dbReference>
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    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012192_0_0_5"/>
    <dbReference type="OMA" id="SAYAHSQ"/>
    <dbReference type="UniPathway" id="UPA00659"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004466">
        <property type="term" value="F:long-chain-acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
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        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015396">
        <property type="entry name" value="DUF1974"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09317">
        <property type="entry name" value="DUF1974"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0274">FAD</keyword>
    <keyword evidence="9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="14">
        <location>
            <begin position="81"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="13">
        <location>
            <begin position="177"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="12">
        <location>
            <begin position="300"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1974" evidence="15">
        <location>
            <begin position="454"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="732"/>
            <end position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00020144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF09317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ABA06245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="755" mass="82858" checksum="7CB1457A31A37E22" modified="2005-10-11" version="1">MSFRRDVISKLIFSWARRVLPSMSDTEREALEAGDVWWDADLFTGNPDWSKLLDTPPATLTAEEQAFLDGPVDELCAMLDEWKINWELRDLPPEVWDFIKQKKFFGMIIPTEHGGLGFSPYAHSEVVRKISSRSLVAAVTVMVPNSLGPGELLMRFGTRDQQNKWLPRLADGRDVPCFGLTSPEAGSDAAAMTDTGIICKGMFEGREVLGLRLNWHKRYITLGPVATLLGLAFKAFDPDHLIGTQEDLGISVALIPTDLPGVSIGRRHLPAMQVFQNGPNWGKDVFIPLDYVIGGKERLGQGWKMLMTALAAGRGISLPSLSAAGAAFAARTAGAYARIREQFGIPIGKFEGVEEPLARIAATAYLIDAARRLTCAALNEGHHPAVISGIMKLHATERLRTVIDDAMDIHGGKAVIDGPQNYLGNLYRGVPVSITVEGANILTRNLIIFGQGAIRAHPYLLHEMNALGDTDKARGLDTFDKTFWDHIVHSFRTLFRAWGRSWSGGAFAPAPDDAGAVTGFYRQLSRYSAAFALCADMALLTLGGKLKRKEMLSARFGDILSELYLLSAVLKRWHEEGRQQADLALVEWCMATGFRTIEQRFADILANLPNRVVAIMLKFMIQPLGAQARGPSDKVIHACAQLVLEPSVARDRLTQGLSNVDDDSGPARLEKAFLLVTAAEDVFKRMRGARLRDADAAVRQRVITQAEAEQHNAAQEAVARVIEVDDFAPEALSPQKEEPVSVQVVHEQTEARAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SND9</accession>
    <name>Q3SND9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Heat shock protein Hsp20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2952</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316124.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2952"/>
    <dbReference type="EnsemblBacteria" id="ABA06202">
        <property type="protein sequence ID" value="ABA06202"/>
        <property type="gene ID" value="Nwi_2952"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2952"/>
    <dbReference type="eggNOG" id="COG0071">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046737_4_2_5"/>
    <dbReference type="OMA" id="NTYRITM"/>
    <dbReference type="OrthoDB" id="1523369at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06470">
        <property type="entry name" value="ACD_IbpA-B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037913">
        <property type="entry name" value="ACD_IbpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="SSF49764"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="4">
        <location>
            <begin position="27"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA06202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="153" mass="16718" checksum="D2B9C4BCCE8030D6" modified="2005-10-11" version="1">MRTFDLTPFYRSTVGFDRLFNLLDQANGDAPGYPPYNIERTGDNAYRISVAVSGFAPNELSIVAKENTLTIKGEKVANENGKEKSEVLYRGIAARAFERAFQLADYVQVKNASLENGLLHVDLVREIPEAKKPRSIPISTGTATAPAVEKAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNP7</accession>
    <name>Q3SNP7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">IstB-like ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2842</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2842"/>
    <dbReference type="EnsemblBacteria" id="ABA06094">
        <property type="protein sequence ID" value="ABA06094"/>
        <property type="gene ID" value="Nwi_2842"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2842"/>
    <dbReference type="eggNOG" id="COG1484">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1961">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1584759_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002611">
        <property type="entry name" value="IstB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01695">
        <property type="entry name" value="IstB_IS21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IstB_IS21" evidence="2">
        <location>
            <begin position="53"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="168" mass="18571" checksum="3C756BA5908F3D7A" modified="2005-10-11" version="1">MVMTDRYACRNHLRKGICDNGRTIRRDEIEARVLAGLRDRLVSSEAVAEAVRAYLPFAQSGGQLLFHLVSRLYERTSIVVTTNLAFGEWPSVFGDAKMTTALLDRLTHHCDIVETGNDSWRFKSRDDDHATRARPVSAIPASSDGPSATLKPRRAKGSNLDADTGSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNQ6</accession>
    <name>Q3SNQ6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Prophage LambdaMc01, site-specific recombinase, resolvase family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2833</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316025.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2833"/>
    <dbReference type="EnsemblBacteria" id="ABA06085">
        <property type="protein sequence ID" value="ABA06085"/>
        <property type="gene ID" value="Nwi_2833"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2833"/>
    <dbReference type="eggNOG" id="COG1961">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1702407_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038109">
        <property type="entry name" value="DNA_bind_recomb_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="154" mass="17259" checksum="6FD0D7610AB45869" modified="2005-10-11" version="1">MTHKGKAYPGEHAAIIDERLWDQVHAILRESPRKRANNSRAQAPALLKGLIFTATGAAMTPSSTKKGARRYRYYVSMDVIRNRETGEEDIPHRLPADTVEAAVVNELRRVMRGPEITAQVIAHLEREGHAFAETDAISALQKFEDVWGQLFPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNU6</accession>
    <name>Q3SNU6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carbonic anhydrase</fullName>
            <ecNumber evidence="3">4.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2792</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + hydrogencarbonate = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta-class carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315990.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2792"/>
    <dbReference type="EnsemblBacteria" id="ABA06045">
        <property type="protein sequence ID" value="ABA06045"/>
        <property type="gene ID" value="Nwi_2792"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2792"/>
    <dbReference type="eggNOG" id="COG0288">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053879_5_3_5"/>
    <dbReference type="OMA" id="WHYIIET"/>
    <dbReference type="OrthoDB" id="1841447at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00884">
        <property type="entry name" value="beta_CA_cladeB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045066">
        <property type="entry name" value="Beta_CA_cladeB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001765">
        <property type="entry name" value="Carbonic_anhydrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036874">
        <property type="entry name" value="Carbonic_anhydrase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11002">
        <property type="entry name" value="PTHR11002"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00484">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00947">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53056">
        <property type="entry name" value="SSF53056"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA06045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="216" mass="23995" checksum="A22F1529070B0586" modified="2005-10-11" version="1">MNGFPQRLIDGYRAFVTQRMPVEQSRYRALSERGQSPETMVIGCCDSRVSPEVIFDARPGELFVVRNIANLVPVYQPDGAAHGISAALEFGVEVLKVKHIVILGHAQCSGISAIIKKAAPLSSGDFIGRWMAMFIKPGEVIEQRERETTKEFATRLEKAAVFRSIENLMTLPFVRRSVESGHLHLHGAYFSIAEGSLYALDQEAREFRSVGDNSLI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNV8</accession>
    <name>RS16_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsP</name>
        <name type="ordered locus">Nwi_2780</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315978.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SNV8"/>
    <dbReference type="SMR" id="Q3SNV8"/>
    <dbReference type="STRING" id="323098.Nwi_2780"/>
    <dbReference type="EnsemblBacteria" id="ABA06033">
        <property type="protein sequence ID" value="ABA06033"/>
        <property type="gene ID" value="Nwi_2780"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2780"/>
    <dbReference type="eggNOG" id="COG0228">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100590_3_1_5"/>
    <dbReference type="OMA" id="GFYNPIA"/>
    <dbReference type="OrthoDB" id="1937072at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00385">
        <property type="entry name" value="Ribosomal_S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000307">
        <property type="entry name" value="Ribosomal_S16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023803">
        <property type="entry name" value="Ribosomal_S16_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12919">
        <property type="entry name" value="PTHR12919"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00886">
        <property type="entry name" value="Ribosomal_S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54565">
        <property type="entry name" value="SSF54565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00002">
        <property type="entry name" value="S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000243835" description="30S ribosomal protein S16">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="82"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="110" mass="12336" checksum="55967A40DFF0566D" modified="2005-10-11" version="1">MPVVIRLARAGTKKRPVYHVVAADSRYPRDGRFIERLGHFNPLMPKDNADRLKLDLDKVKAWLAKGAQPSDRVARFLDAAGVKKRTPRNNPVKAVPRKERKAQAEAAAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNW1</accession>
    <name>Q3SNW1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcription activator, effector binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2777</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315975.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2777"/>
    <dbReference type="EnsemblBacteria" id="ABA06030">
        <property type="protein sequence ID" value="ABA06030"/>
        <property type="gene ID" value="Nwi_2777"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2777"/>
    <dbReference type="eggNOG" id="COG4978">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081271_0_0_5"/>
    <dbReference type="OMA" id="EAKDTFI"/>
    <dbReference type="OrthoDB" id="1748122at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010499">
        <property type="entry name" value="AraC_E-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029442">
        <property type="entry name" value="GyrI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011256">
        <property type="entry name" value="Reg_factor_effector_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06445">
        <property type="entry name" value="GyrI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00871">
        <property type="entry name" value="AraC_E_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55136">
        <property type="entry name" value="SSF55136"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AraC_E_bind" evidence="2">
        <location>
            <begin position="93"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="242" mass="25880" checksum="CEA0EDC57D9CFB12" modified="2005-10-11" version="1">MNRLRFFFPLGIAAISGVMLLGLSALLAETPPATPPGATAPAAEAPPAAPAPPGTTAPATPTPPPAEVQTPENPTAEAPKPPATAPVQTADPFGEEHTLTPKTVLSLRNTATWDTAFETLIDSFKSLAALLDKRGVKPAGNMMIVYTSTDDNGFSFLAEIPVDQEIKDLPKNMSMGKSPEGKALKFVHRGSYDNMDNTYEAITNTLDDRKLEAKDTFIEEYTTDPLKTEEDSLVIDVYVPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNX4</accession>
    <name>Q3SNX4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Adenylyl-sulfate kinase</fullName>
            <ecNumber evidence="11">2.7.1.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">APS kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">ATP adenosine-5'-phosphosulfate 3'-phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Adenosine-5'-phosphosulfate kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">cysC</name>
        <name evidence="13" type="ordered locus">Nwi_2764</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">APS kinase catalyzes the synthesis of activated sulfate.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Catalyzes the synthesis of activated sulfate.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Proposed to provide activated sulfate for transfer to Nod factor. ATP sulfurylase may be the GTPase, regulating ATP sulfurylase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H(+) + sulfate = adenosine 5'-phosphosulfate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18133"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="EC" id="2.7.7.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 11">
            <text>adenosine 5'-phosphosulfate + ATP = 3'-phosphoadenylyl sulfate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24152"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="11">Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Sulfate-activating enzymes, NodP and NodQ, may be physically associated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the APS kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the APS kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the N-terminal section; belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. CysN/NodQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.25" evidence="11"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315962.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2764"/>
    <dbReference type="EnsemblBacteria" id="ABA06017">
        <property type="protein sequence ID" value="ABA06017"/>
        <property type="gene ID" value="Nwi_2764"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2764"/>
    <dbReference type="eggNOG" id="COG0529">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2895">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007265_5_3_5"/>
    <dbReference type="OMA" id="DWYGGPT"/>
    <dbReference type="OrthoDB" id="244339at2"/>
    <dbReference type="UniPathway" id="UPA00140">
        <property type="reaction ID" value="UER00205"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004020">
        <property type="term" value="F:adenylylsulfate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004781">
        <property type="term" value="F:sulfate adenylyltransferase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070814">
        <property type="term" value="P:hydrogen sulfide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02027">
        <property type="entry name" value="APSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04095">
        <property type="entry name" value="CysN_NoDQ_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00065">
        <property type="entry name" value="Adenylyl_sulf_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002891">
        <property type="entry name" value="APS_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044139">
        <property type="entry name" value="CysN_NoDQ_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="SSF50465"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00455">
        <property type="entry name" value="apsK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="11 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tr-type G" evidence="12">
        <location>
            <begin position="22"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="11">
        <location>
            <position position="540"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="466"/>
            <end position="473"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA06017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="641" mass="68776" checksum="1DFD1CA57B9AE0B2" modified="2005-10-11" version="1">MNVIASNLASNAALATPNGTTRPQVSIVIVGHVDHGKSTLVGRLLHETGSLPDGKLEMLKAVSARRGMPFEWSFLLDALQTERDQGITIDTTQIRFRTRSRDVVLIDAPGHAEFLRNMITGAAQADGAVLIIDALEGVRDQTRRHGYLLHLLGVKQVAVVVNKMDRVDFSAERFGAIRDEISAHLTGLGLAPTAVIPISARDGDGVAERTPKIDWYDGPVVVEAIDALQPARPLDELALRLPVQAIYKFDDRRIVAGRIESGGLSAGDEIVIMPAGKIANIRSVEGWPSSPTGAQGAGRSVGITLDRELFVERGDVIAHVGSAPRDTRRIRARIFWLHEQPLAAGASILLRLGNKETRAVVVAIEKAVDPGELSSAETKAIARNHVGEIDISLAQPLAADPYVDNPRTGRLVIEVNGRIAGGGLVLSVDAGQRAVPVDIVPVESALRPEERSARYRHNGAVVWLTGLPGSGKSTLARALERKLFSDGGSPILLDGDTLRAGLNGDLGFSPEDRAENIRRLAEIATHLARNGHIAIVAAVSPSREDRAAARRIADDTFREIYVATPADVCERRDPKGHYAKARAGVLHGFTGIGNDYQPPESVELTIDTSSCTVSDAADEIERMLIRTGVLFDEVTDIAANI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP00</accession>
    <name>Q3SP00_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Thiamine monophosphate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2738</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315936.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2738"/>
    <dbReference type="EnsemblBacteria" id="ABA05991">
        <property type="protein sequence ID" value="ABA05991"/>
        <property type="gene ID" value="Nwi_2738"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2738"/>
    <dbReference type="eggNOG" id="COG0352">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018272_3_1_5"/>
    <dbReference type="OMA" id="RPDYIFF"/>
    <dbReference type="OrthoDB" id="1558094at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00564">
        <property type="entry name" value="TMP_TenI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036206">
        <property type="entry name" value="ThiamineP_synth_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022998">
        <property type="entry name" value="ThiamineP_synth_TenI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02581">
        <property type="entry name" value="TMP-TENI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51391">
        <property type="entry name" value="SSF51391"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TMP-TENI" evidence="2">
        <location>
            <begin position="15"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="233" mass="24182" checksum="20B6C3397482752D" modified="2005-10-11" version="1">MPPKAAPPRPAPRLYLATPVIDDPSHVAAQLPALLASADVAAVLVRLSPSDPRTMISRIKALAPLIQSTGVALLLEAHADLVARAGADGAHLTGIEALQETLPVLKPDRIAGVGGLSTRHDSMVAGEAGADYVLFGEPDETGDRPSAEAIAERLTWWAELFEPPCVAHAMNIDEARLFAASGADFILVDDIVWTDTRGAALALADIATAIARGHESQGGAERTDPPHGVRGAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP42</accession>
    <name>Q3SP42_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">Threonylcarbamoyl-AMP synthase</fullName>
            <shortName evidence="7">TC-AMP synthase</shortName>
            <ecNumber evidence="4 7">2.7.7.87</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 7">L-threonylcarbamoyladenylate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_2696</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP + hydrogencarbonate + L-threonine = diphosphate + H2O + L-threonylcarbamoyladenylate</text>
            <dbReference type="Rhea" id="RHEA:36407"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="EC" id="2.7.7.87"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the SUA5 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.87" evidence="4 7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315895.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2696"/>
    <dbReference type="EnsemblBacteria" id="ABA05949">
        <property type="protein sequence ID" value="ABA05949"/>
        <property type="gene ID" value="Nwi_2696"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2696"/>
    <dbReference type="eggNOG" id="COG0009">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031397_0_2_5"/>
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    <dbReference type="OrthoDB" id="1913202at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061710">
        <property type="term" value="F:L-threonylcarbamoyladenylate synthase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
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        <property type="entry name" value="Sua5/YwlC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005145">
        <property type="entry name" value="Sua5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010923">
        <property type="entry name" value="T(6)A37_SUA5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="YrdC-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03481">
        <property type="entry name" value="SUA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004930">
        <property type="entry name" value="Tln_factor_SUA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="SSF55821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00057">
        <property type="entry name" value="TIGR00057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="YrdC-like" evidence="9">
        <location>
            <begin position="14"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR004930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR004930-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA05949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="328" mass="33588" checksum="6DFD0B81013532B4" modified="2005-10-11" version="1">MTVDLKTQILPAGETAAAAAARILHNGGLVAFPTETVYGLGADAANAEAVARLYRAKGRPAFNPLIAHVSDLAAAQRIARFDAAALALTEAFWPGPLTLVLPKVARCPVADLATASLDTIALRVPAHPVARAILRGFGGAVVAPSANLSGHVSPTTAAHVRHDLEGRIDLIVDGGPVEVGIESTIVGCFDRPTLLRPGGISRADIERVLGGPLVSPAPAAADGRPRASGMLASHYAPRARMRLNAANLAPGEALLAFGPVDPPGIDTAVAVMNLSERGDLKEAAAHLFGCLRALDASGARTIAVTPIPEDGLGEAINDRLRRAAAPKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP78</accession>
    <name>Q3SP78_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2660</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315863.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05913">
        <property type="protein sequence ID" value="ABA05913"/>
        <property type="gene ID" value="Nwi_2660"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2660"/>
    <dbReference type="eggNOG" id="ENOG5032V77">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126505_0_0_5"/>
    <dbReference type="OMA" id="WIYDYIG"/>
    <dbReference type="OrthoDB" id="1899662at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="134" mass="14370" checksum="A05F44133C32057C" modified="2005-10-11" version="1">MMSNPFVGNWSYRSFLNDPNLNKPANDLLFGAGTLTIVERSETELSGTIGGSGWSLDLRGSFGYGSPMQVRFQGKGTVGGEPWIYDYIGWLVPVWPNSTDQLEVPAIVGSVVRTIPHGASQAGVVASFYAVRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPA5</accession>
    <name>Q3SPA5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NUDIX hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2633</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315836.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2633"/>
    <dbReference type="EnsemblBacteria" id="ABA05886">
        <property type="protein sequence ID" value="ABA05886"/>
        <property type="gene ID" value="Nwi_2633"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2633"/>
    <dbReference type="eggNOG" id="COG1051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037162_10_1_5"/>
    <dbReference type="OMA" id="LYWRFAR"/>
    <dbReference type="OrthoDB" id="1308416at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="2">
        <location>
            <begin position="27"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="162" mass="18601" checksum="6BA74E2A1CB87F2A" modified="2005-10-11" version="1">MNFHRLRRFEESARRRLFHLYFRFARGMTLGVRGVVLDADNRVFLVRHGYVAGWHLPGGGVEVGEASLTALERELMEEGRIEICGEPVLHGIFFNSHVSRRDHVTVYVVRDFRQDRKPEPNREIIDCGFFAADALPADTTAGTRLRIAEVLYHEAPVPTWRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPC6</accession>
    <name>Q3SPC6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Heat shock protein, Metallo peptidase, MEROPS family M48B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_2612</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M48 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315815.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2612"/>
    <dbReference type="EnsemblBacteria" id="ABA05865">
        <property type="protein sequence ID" value="ABA05865"/>
        <property type="gene ID" value="Nwi_2612"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2612"/>
    <dbReference type="eggNOG" id="COG0501">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042266_2_0_5"/>
    <dbReference type="OMA" id="REYMADS"/>
    <dbReference type="OrthoDB" id="1918093at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 6" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0346">Stress response</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="19"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="177"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M48" evidence="9">
        <location>
            <begin position="99"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="329"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="348"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA05865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="383" mass="41391" checksum="7D75D47434F7D3A5" modified="2005-10-11" version="1">MAAYGLYTHIASNKIRSMFLLGGLFVLIYVMVFVGALIAEVFRLGADYPAGFYIRQAWHDLIVALPWATAGSVLWIVIAYFFHQSIIDAVTGGHDVSRKEQPRLYNLLENLCISRGIPMPRLKVMDTDALNAFATGLNRRQYAITVTTGLMNGLNDEEMEAVLGHELTHIRNGDVQLMVVAVIIAGVVGFVAELCFRTFLRSGARLSGGSGRTSRSSSSRGGKSGGAVIAVILAMVLIVLAWFLSQVVRMALSRSRELMADAGSVELTKNPDAMISALRKIENRGELPGVTSAVMEMCVDNPRQGFADLFATHPSVDTRVRALVRYAGGRDPGPMQLPAESAVADPELPDLPPGPWSKPPIPNATTPDAAPPPSVPDPWSHRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPD0</accession>
    <name>Q3SPD0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chromosome partition protein Smc</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">smc</name>
        <name evidence="6" type="ordered locus">Nwi_2608</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for chromosome condensation and partitioning.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="3">Contains large globular domains required for ATP hydrolysis at each terminus and a third globular domain forming a flexible hinge near the middle of the molecule. These domains are separated by coiled-coil structures.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315811.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2608"/>
    <dbReference type="EnsemblBacteria" id="ABA05861">
        <property type="protein sequence ID" value="ABA05861"/>
        <property type="gene ID" value="Nwi_2608"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2608"/>
    <dbReference type="eggNOG" id="COG1196">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001042_2_2_5"/>
    <dbReference type="OMA" id="HNKIAME"/>
    <dbReference type="OrthoDB" id="1149850at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01894">
        <property type="entry name" value="Smc_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011890">
        <property type="entry name" value="SMC_prok"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="SSF75553"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02168">
        <property type="entry name" value="SMC_prok_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC_N" evidence="5">
        <location>
            <begin position="3"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="453"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="170"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="637"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="769"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="949"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="32"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1168" mass="127914" checksum="F4963057482DC9BC" modified="2005-10-11" version="1">MKLTRLRLHGFKSFVEPTDFVIAPGLTGVVGPNGCGKSNLVEALRWAMGETSYKSLRAADMDAVIFAGSGNRPARNHAEVVMSIDNSDHSAPAAFNESEALEISRRIERESGSVYRINGKDVRARDVQILFADAATGARSPALVHQGKIGEIIQAKPEQRRRVLEDAAGVAGLHARRHEAELRLKAAETNLTRVEDVIGQLAGQMEGLKKQARQAIRYRDVAAKVRKAEATLFHLRWLEAQAEVAEAARTHDLNVRELAERTREQAEAARLQAERAAELPALREAEARAAAGLQRLINARETLDREEERAKERAAELDHRLTQFASDVEREQRQSSDADAALQRLDVEDTELKDEIKSRVETRSGVDERAAQAEATLAAAEATFADLTRVLADLTAKRNQLEANARTHRDRLARIDQQIADVQRETEALAQQTSGFGDLDALTAAIENAQQALAQSESSSREREAEHAAARGQLDAARVGVEAARGPLQDADKRAQRLETEAKTIAKILNGESKNLWPPIIDGVSVDKGYEKAIGAVLGDDLDAPVDPSAPMRWTGADVTADDPVLPEGVESLAAHVQAPPELARRLEQIGVVARERGAELVADLKTGQRLVSLEGDVWRWDGFVTAAHAPTGAARRLAERARLADIESEIERARSEVSERRQALEAAQQAVVAASQTVAAAASAESDARNAWRAAQREVDAARERHARAEREINRHAARRSALTEAHSRLTNDRLEAASAHEAATSALGELPPSLDTETKLGEVRAEIDGHRRAAAQVRAEAQALAREAELADRRLQAISAERNEWQTRKQSAASQIATIEARVTEVKAERADLDNAPALFAEKRSAIISEIEHAQRDRREAADALAAAEAVMAETDRAARVSLEALSSAREATARAEERMDGSKRRLEDTEREIRDMLEVEPDAVASLAEIQSGVDLPPLPEIEADLEKLRRDRDRLGAVNLRAEEELREVDTQHGSLATERDDLVEAIKKLRTGIQSLNREARERLLTSFETVNTHFKRLFTDLFGGGEAALHLIESDDPLEAGLEIIAKPPGKKPQTLSLLSGGEQALTALALIFAVFLTNPSPICVLDEVDAPLDDHNVERFCNLLHEMTSSTETRFIIITHNPITMARMNRLFGVTMAERGVSQLVSVDLENAVKILDQHVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPD3</accession>
    <name>Q3SPD3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Adenine DNA glycosylase</fullName>
            <ecNumber evidence="4 14">3.2.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">Nwi_2605</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Adenine glycosylase active on G-A mispairs. MutY also corrects error-prone DNA synthesis past GO lesions which are due to the oxidatively damaged form of guanine: 7,8-dihydro-8-oxoguanine (8-oxo-dGTP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>Hydrolyzes free adenine bases from 7,8-dihydro-8-oxoguanine:adenine mismatched double-stranded DNA, leaving an apurinic site.</text>
            <dbReference type="EC" id="3.2.2.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="14">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the Nth/MutY family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.31" evidence="4 14"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315808.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2605"/>
    <dbReference type="EnsemblBacteria" id="ABA05858">
        <property type="protein sequence ID" value="ABA05858"/>
        <property type="gene ID" value="Nwi_2605"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2605"/>
    <dbReference type="eggNOG" id="COG1194">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012862_0_2_5"/>
    <dbReference type="OMA" id="RDPYRVW"/>
    <dbReference type="OrthoDB" id="1274533at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000701">
        <property type="term" value="F:purine-specific mismatch base pair DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03431">
        <property type="entry name" value="DNA_Glycosylase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004036">
        <property type="entry name" value="Endonuclease-III-like_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003651">
        <property type="entry name" value="Endonuclease3_FeS-loop_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000445">
        <property type="entry name" value="HhH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044298">
        <property type="entry name" value="MIG/MutY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029119">
        <property type="entry name" value="MutY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42944">
        <property type="entry name" value="PTHR42944"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00633">
        <property type="entry name" value="HHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14815">
        <property type="entry name" value="NUDIX_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00525">
        <property type="entry name" value="FES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01155">
        <property type="entry name" value="ENDONUCLEASE_III_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8 14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="13 14 17" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="9 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 14" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENDO3c" evidence="16">
        <location>
            <begin position="57"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="189"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="196"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU365096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ABA05858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="415" mass="45654" checksum="F5A6E4B368FF4F9D" modified="2005-10-11" version="1">MARAATAIRHTHKMQGGDRNRPVLLLEWYDRNRRLLPWRALPGEPVDPYRVWLSEIMLQQTTVKTVGPYFEKFLARWPDVAAMARASLDDILRMWAGLGYYSRARNLHACAVKVLRDHGGRFPDTEEDLRALPGIGPYTAAAIAAIAFNRRTMPVDGNIERVVSRLFAVDEPLPKAKPRIHTLAATLLGPSRSGRDGKSRAGDVKTRAGRDGKSRASDVKTRAGDIAQALMDLGAAICTPKKPSCVLCPLSDDCAARARGDQETFPRKTPKKAGELRRGAAFVVRRGSEVLVRTRPAKGLLGGMTEVPTSTWLAAQDDTAALKQAPCLESAPRWRRKAGAVTHVFTHFPLELAVYTAAVARRTAAPEGMRWVPIARLNDEALPNLMRKVIAHGLGDEMEIHALATRRKQQEGPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPG9</accession>
    <name>Q3SPG9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2569</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315773.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2569"/>
    <dbReference type="EnsemblBacteria" id="ABA05822">
        <property type="protein sequence ID" value="ABA05822"/>
        <property type="gene ID" value="Nwi_2569"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2569"/>
    <dbReference type="eggNOG" id="COG3416">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082335_2_0_5"/>
    <dbReference type="OMA" id="MAQAMII"/>
    <dbReference type="OrthoDB" id="1972954at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018648">
        <property type="entry name" value="DUF2076"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09849">
        <property type="entry name" value="DUF2076"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="265" mass="27431" checksum="12D892962C35B337" modified="2005-10-11" version="1">MTPEERQLVDDLFTRLAGVENAPRDPAAAAAIAEGLRRAPNALYTLVQTVLVQDEALRRANDRIQELESGGTADQAAPGGFLDQMRDTIFGKPAPRGSVPNVQPPPIPSERPAWNTGEALQQTGQAGRVDQGRFDQGSGRQPYGAPQPQAGGGSFLGTAAAAAAGAVGGSLLLNSIRSIMGGGEAHAAGSGLTGSSSNPWSGAGDQSGSDLARDAGVKDLGSHKQDDPSQAGPFDHAHGDDYHDQDDIDSDDFDDFDDLDDGDYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPI0</accession>
    <name>Q3SPI0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2558</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315762.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2558"/>
    <dbReference type="EnsemblBacteria" id="ABA05811">
        <property type="protein sequence ID" value="ABA05811"/>
        <property type="gene ID" value="Nwi_2558"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2558"/>
    <dbReference type="eggNOG" id="COG0483">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_174701_0_0_5"/>
    <dbReference type="OMA" id="MRYFVVH"/>
    <dbReference type="OrthoDB" id="1971049at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025226">
        <property type="entry name" value="DUF4170"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13773">
        <property type="entry name" value="DUF4170"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="83" mass="9299" checksum="DE6EA6E8395B28F6" modified="2005-10-11" version="1">MPSDSPPQQLLHLVIGGELTDLAHTAFKNLDEVEIVGLYPNYASAYAAWKAKAQRTVDNAHTRYFIVHLHRLLDPGQDTKTDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPJ5</accession>
    <name>Q3SPJ5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Lipoprotein signal peptidase</fullName>
            <ecNumber evidence="6">3.4.23.36</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Prolipoprotein signal peptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Signal peptidase II</fullName>
            <shortName evidence="6">SPase II</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">lspA</name>
        <name evidence="9" type="ordered locus">Nwi_2543</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">This protein specifically catalyzes the removal of signal peptides from prolipoproteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains.</text>
            <dbReference type="EC" id="3.4.23.36"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Protein modification; lipoprotein biosynthesis (signal peptide cleavage).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell inner membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 8">Belongs to the peptidase A8 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.23.36" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315747.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2543"/>
    <dbReference type="EnsemblBacteria" id="ABA05796">
        <property type="protein sequence ID" value="ABA05796"/>
        <property type="gene ID" value="Nwi_2543"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2543"/>
    <dbReference type="eggNOG" id="COG0597">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083252_4_3_5"/>
    <dbReference type="OMA" id="ASKLWLY"/>
    <dbReference type="OrthoDB" id="1575081at2"/>
    <dbReference type="UniPathway" id="UPA00665"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00161">
        <property type="entry name" value="LspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001872">
        <property type="entry name" value="Peptidase_A8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33695">
        <property type="entry name" value="PTHR33695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01252">
        <property type="entry name" value="Peptidase_A8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00781">
        <property type="entry name" value="LIPOSIGPTASE"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00077">
        <property type="entry name" value="lspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00855">
        <property type="entry name" value="SPASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="6" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2 6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 7" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="165" mass="17493" checksum="9575EBE5F2F7CD0D" modified="2005-10-11" version="1">MTPAVRAGIAAAVAALIADQASKLWLLFAFDIGRRGAVRVTPFFDLVLAWNTGISYGWFQTDSPVGAAVLLVVKALAVVLLAVWMARSHTHMATIGLGLIIGGAIGNAIDRFAYGAVMDFALFHLQYAGQTYSWYIFNLADVAIVVGVIALLYDSFIGTSAAKAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPK3</accession>
    <name>Q3SPK3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2535</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2535"/>
    <dbReference type="EnsemblBacteria" id="ABA05788">
        <property type="protein sequence ID" value="ABA05788"/>
        <property type="gene ID" value="Nwi_2535"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2535"/>
    <dbReference type="eggNOG" id="COG4655">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022237_2_0_5"/>
    <dbReference type="OMA" id="LSVMDYN"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018705">
        <property type="entry name" value="DUF2134_membrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028087">
        <property type="entry name" value="Tad_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13400">
        <property type="entry name" value="Tad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09977">
        <property type="entry name" value="Tad_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="Tad" evidence="3">
        <location>
            <begin position="14"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Tad_C" evidence="2">
        <location>
            <begin position="62"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="554" mass="55615" checksum="1F327D41C9D7B2B5" modified="2005-10-11" version="1">MRNLLRRFISDTRGNFAIMGAGCMVLVLGCTALGVDVGAIYADRRKAQSAADLAAIVAAGNLGKAASAAAATVTANGYPARALLSVELGTFTANAALTPQARFVTPATGAPNAARVTLRTETPLYFARMFAGGVNHFDITTRATAASTALASFAIGSRLLALNGGLLNAVLGRMLGTTLSLSVMDYQALIDARIDAFDFLNALATRLDLTGVTYDSMLSGEVKVADIVAAMLSAQQAANGINAATAALSNVSLALAGLSTTIVPGKLLDVGPYMPMTVGSKPKTGVGVSVFDLLSATGAIANGTNQIAAAADLGLPGIAAVSVRATIGERPQGKSWMTVGTEGASVHTAQTRVLVTIKLIGAGAVSAVNLPLYVEVASGTATLDGVSCGRPDIATSSVTLGVTPGIVDAWIGDVSVAEMTNFTSKPDPGAATLVNLGAITVTGRAHAGMGNTQPTAVSFSYSDIVARTKKTVTTTDFTSSLTASLLGDLSLKVNVGPLGLPIPGLGPAVAGIISGATGSIDQVLAQVLATLGIGIGQADVWVSGIRCDGAVLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPM5</accession>
    <name>Q3SPM5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2513</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315717.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2513"/>
    <dbReference type="EnsemblBacteria" id="ABA05766">
        <property type="protein sequence ID" value="ABA05766"/>
        <property type="gene ID" value="Nwi_2513"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2513"/>
    <dbReference type="eggNOG" id="COG5465">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109255_0_0_5"/>
    <dbReference type="OMA" id="FVVWAGK"/>
    <dbReference type="OrthoDB" id="1416612at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019660">
        <property type="entry name" value="Put_sensory_transdc_reg_YbjN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10722">
        <property type="entry name" value="YbjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="166" mass="18361" checksum="3E34B8C23886EE4A" modified="2005-10-11" version="1">MPLLEGAIDSKNNPLAVVEDIAATNDWAFERSGEDEITIVSKGDWTDYQLTFTWMGEIDALHLACAFDMKIPVARRNEAQRAIAAINEQLWIGHFDIWTHTGTIMYRHALILPEGLTASNAQCAEMLANAIHACERYYPALQFVVWAGKSAPEAMTAAMFDTAGEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPM8</accession>
    <name>Q3SPM8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome-binding ATPase YchF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ychF</name>
        <name evidence="8" type="ordered locus">Nwi_2510</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">ATPase that binds to both the 70S ribosome and the 50S ribosomal subunit in a nucleotide-independent manner.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. YchF/OLA1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315714.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2510"/>
    <dbReference type="EnsemblBacteria" id="ABA05763">
        <property type="protein sequence ID" value="ABA05763"/>
        <property type="gene ID" value="Nwi_2510"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2510"/>
    <dbReference type="eggNOG" id="COG0012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018395_0_1_5"/>
    <dbReference type="OMA" id="VLRCFDN"/>
    <dbReference type="OrthoDB" id="603226at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04867">
        <property type="entry name" value="TGS_YchF_OLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01900">
        <property type="entry name" value="YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00944">
        <property type="entry name" value="YchF_OLA1_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004396">
        <property type="entry name" value="ATPase_YchF/OLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023192">
        <property type="entry name" value="TGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013029">
        <property type="entry name" value="YchF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041706">
        <property type="entry name" value="YchF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06071">
        <property type="entry name" value="YchF-GTPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006641">
        <property type="entry name" value="CHP00092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="SSF81271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00092">
        <property type="entry name" value="TIGR00092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="6">
        <location>
            <begin position="3"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="7">
        <location>
            <begin position="280"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="131"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="12"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA05763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="365" mass="39367" checksum="A5073693E2C66532" modified="2005-10-11" version="1">MGFKCGIVGLPNVGKSTLFNALTETAAAQAANYPFCTIEPNVGAVAVPDPRLGRLAEVAKSAQIIPTQLTFVDIAGLVRGASKGEGLGNQFLANIREVDAVAHVVRCFEDSDITHVEGKIAPLADIETIETELMLADLESLEKRVDNLSKKAKGNDKEAREQLDLVERALSLLRDGQPTRGMERKPEEERNFRMLGLLTSKPVLYVCNVEESSAAGGNAFSQAVFDHAAKEGAIAVVISAKIESEIATLSRDERADFLETLGLEEAGLDRLIRAGYRLLDLITYFTVGPKEARAWTITRGTKAPAAAGVIHTDFEKGFIRAETIAYADYAALGGEAGARDAGKLRLEGKEYVVADGDVMHFRFNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPM9</accession>
    <name>Q3SPM9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2509</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315713.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2509"/>
    <dbReference type="EnsemblBacteria" id="ABA05762">
        <property type="protein sequence ID" value="ABA05762"/>
        <property type="gene ID" value="Nwi_2509"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2509"/>
    <dbReference type="eggNOG" id="ENOG50332K3">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_169571_1_2_5"/>
    <dbReference type="OMA" id="LLFRIHD"/>
    <dbReference type="OrthoDB" id="1901821at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025557">
        <property type="entry name" value="DUF4282"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14110">
        <property type="entry name" value="DUF4282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="98" mass="10840" checksum="624852B40DD60F6E" modified="2005-10-11" version="1">MFEVRDLFQWDRFITPTIIKVFYWLVIVLIVLSGLSGIFAGLSSMAVSPFSGFITILLNVAGVLVGILFSRIAAESILIVFRINEHLGAIRDQGATRP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPS0</accession>
    <name>THIC_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphomethylpyrimidine synthase</fullName>
            <ecNumber evidence="1">4.1.99.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Hydroxymethylpyrimidine phosphate synthase</fullName>
            <shortName evidence="1">HMP-P synthase</shortName>
            <shortName evidence="1">HMP-phosphate synthase</shortName>
            <shortName evidence="1">HMPP synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Thiamine biosynthesis protein ThiC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thiC</name>
        <name type="ordered locus">Nwi_2468</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + S-adenosyl-L-methionine = 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + 5'-deoxyadenosine + CO + formate + 3 H(+) + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:24840"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:17245"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58354"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="EC" id="4.1.99.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ThiC family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.17" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315676.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SPS0"/>
    <dbReference type="SMR" id="Q3SPS0"/>
    <dbReference type="STRING" id="323098.Nwi_2468"/>
    <dbReference type="PRIDE" id="Q3SPS0"/>
    <dbReference type="EnsemblBacteria" id="ABA05721">
        <property type="protein sequence ID" value="ABA05721"/>
        <property type="gene ID" value="Nwi_2468"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2468"/>
    <dbReference type="eggNOG" id="COG0422">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013181_2_1_5"/>
    <dbReference type="OMA" id="TWELFRD"/>
    <dbReference type="OrthoDB" id="505395at2"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016830">
        <property type="term" value="F:carbon-carbon lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00089">
        <property type="entry name" value="ThiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037509">
        <property type="entry name" value="ThiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025747">
        <property type="entry name" value="ThiC-associated_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038521">
        <property type="entry name" value="ThiC/Bza_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002817">
        <property type="entry name" value="ThiC/BzaA/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30557">
        <property type="entry name" value="PTHR30557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13667">
        <property type="entry name" value="ThiC-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01964">
        <property type="entry name" value="ThiC_Rad_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00407">
        <property type="entry name" value="phosphomethylpyrimidine_syntha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00190">
        <property type="entry name" value="thiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000242276" description="Phosphomethylpyrimidine synthase">
        <location>
            <begin position="1"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="335"/>
            <end position="337"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="376"/>
            <end position="379"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="415"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="419"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="442"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="483"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="563"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="566"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="571"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="646" mass="71097" checksum="558351E016AD478E" modified="2005-10-11" version="1">MNIRSNPDTTRPAVTTGALPSSRKMFSAPDAAPDLRVPLREILLSEGAGEPNLPVYDTSGPYTDPNVIIDVNAGLPRTRLAWVKERGGVEEYDGREIKPEDNGNVGASHAAAAFKAHHKPLRGIGDAPITQLEFARAGIITKEMIYVAERENLGRKKQLERAEAALADGEAFGASVPAFITPEFVREEIARGRAIIPSNINHAELEPMIIGRNFLVKINANIGNSAVTSSVEEEVDKMVWAIRWGADTVMDLSTGRNIHTTREWILRNSPVPIGTVPIYQALEKCDGDPVKLTWELYRDTLVEQCEQGVDYFTIHAGVRLPYIHLTADRVTGIVSRGGSIMAKWCLAHHKESFLYTHFEEICDLMRKYDVSFSLGDGLRPGSIADANDRAQFAELETLGELTQIAWKKGCQVMIEGPGHVPMHKIKINMDKQLKECGEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGCAMLCYVTPKEHLGLPNRDDVKTGVITYKIAAHAADLAKGHPAAQLRDDALSRARFDFRWQDQFNLGLDPDTAVAFHDETLPKDAHKVAHFCSMCGPKFCSMKITQDVRDYAATLGDNEKAALYPDTAPKANDAAGVPEPSYRDQGMKEMSARFKEMGGNVYLDAEKVKESNRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPT5</accession>
    <name>Q3SPT5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thioredoxin reductase</fullName>
            <ecNumber evidence="3">1.8.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_2453</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>[thioredoxin]-dithiol + NADP(+) = [thioredoxin]-disulfide + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:20345"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="4">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.9" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315661.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2453"/>
    <dbReference type="EnsemblBacteria" id="ABA05706">
        <property type="protein sequence ID" value="ABA05706"/>
        <property type="gene ID" value="Nwi_2453"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2453"/>
    <dbReference type="eggNOG" id="COG0492">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031864_5_1_5"/>
    <dbReference type="OMA" id="VDNFPGY"/>
    <dbReference type="OrthoDB" id="692968at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019430">
        <property type="term" value="P:removal of superoxide radicals"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008255">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_2_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005982">
        <property type="entry name" value="Thioredox_Rdtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01292">
        <property type="entry name" value="TRX_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00573">
        <property type="entry name" value="PYRIDINE_REDOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 3" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="5">
        <location>
            <begin position="8"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="321" mass="34133" checksum="8381CE29E218AAEC" modified="2005-10-11" version="1">MPAPIHAKVVIIGSGPAGYTAAIYAARAMLEPVLIQGIQPGGQLTITTDVENYPGFADVIQGPWLMEQMEKQAAHVGTRIVTDTVNSLDVSQRPFRLTCDSGEVYLAETVILATGAQARWLGLPSEDTFKGFGVSACATCDGFFYRGKNVIVIGGGNTAVEEALFLTNFASQVTLVHRRDHFRAERILQDRAFKHPKIKVIWDSAIDEICGSEDPPKVTHVRLKNVKSGELTEVEADGVFIAIGHAPATELVTGKVKLKPSGYVEVAPNSTATSVPGLFAAGDVADEVYRQAVTAAGLGCMAALEAERFLAAHAGDRAAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPW8</accession>
    <name>Q3SPW8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Adenosylmethionine-8-amino-7-oxononanoate aminotransferase</fullName>
            <ecNumber evidence="4">2.6.1.62</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">7,8-diamino-pelargonic acid aminotransferase</fullName>
            <shortName evidence="4">DAPA AT</shortName>
            <shortName evidence="4">DAPA aminotransferase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">7,8-diaminononanoate synthase</fullName>
            <shortName evidence="4">DANS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Diaminopelargonic acid synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bioA</name>
        <name evidence="6" type="ordered locus">Nwi_2419</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA). It is the only aminotransferase known to utilize SAM as an amino donor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(8S)-8-amino-7-oxononanoate + S-adenosyl-L-methionine = (7R,8S)-7,8-diammoniononanoate + S-adenosyl-4-methylsulfanyl-2-oxobutanoate</text>
            <dbReference type="Rhea" id="RHEA:16861"/>
            <dbReference type="ChEBI" id="CHEBI:16490"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:149468"/>
            <dbReference type="ChEBI" id="CHEBI:149469"/>
            <dbReference type="EC" id="2.6.1.62"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; biotin biosynthesis; 7,8-diaminononanoate from 8-amino-7-oxononanoate (SAM route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. BioA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.62" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315631.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2419"/>
    <dbReference type="EnsemblBacteria" id="ABA05673">
        <property type="protein sequence ID" value="ABA05673"/>
        <property type="gene ID" value="Nwi_2419"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2419"/>
    <dbReference type="eggNOG" id="COG0161">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_4_3_5"/>
    <dbReference type="OMA" id="VAVKMCL"/>
    <dbReference type="OrthoDB" id="478143at2"/>
    <dbReference type="UniPathway" id="UPA00078">
        <property type="reaction ID" value="UER00160"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004015">
        <property type="term" value="F:adenosylmethionine-8-amino-7-oxononanoate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00834">
        <property type="entry name" value="BioA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005815">
        <property type="entry name" value="BioA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00508">
        <property type="entry name" value="bioA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4" id="KW-0093">Biotin biosynthesis</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="4">
        <location>
            <begin position="106"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="4">
        <location>
            <begin position="296"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="385"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Participates in the substrate recognition with KAPA and in a stacking interaction with the adenine ring of SAM" evidence="4">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="4">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="419" mass="45541" checksum="144CB2DDC2EDDFBF" modified="2005-10-11" version="1">MKLASPVWHPFTQHALQPEAMPIASGEGAWLETADGRRIFDAISSWWVVTHGHRHPRIVQAIKDQTDRLDQVIFAGFTHEPAERLARLLVAMTPPGLDYVFFSDSGSTSVEVALKMALGFWRNRGERRSRILALQGAYHGDTIGGMSVGERGVFNAPYDPLLFDVERIPFPSSDRAQATLDAITMACRNSGVAALIVEPLILGAGGMLIYPAPVLAEMKRICEAHGVLFIADEVMTGWGRTGTLFACEQAGVTPDIACYSKGLTGGTLPLAVTLCRAGIFDAHYSTDRGKTFFHSSSYTANPIACAAACANLEIWRSEPVMERIAAVARAQTSGLDRFREDRRFINVRQIGTIAALDVVADDAGYLAGIGPRLYDHFLSRGLLVRPLGNTIYLMPPYCSTPQELDSVFDAISEIADAVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ08</accession>
    <name>Q3SQ08_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2379</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315594.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05633">
        <property type="protein sequence ID" value="ABA05633"/>
        <property type="gene ID" value="Nwi_2379"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2379"/>
    <dbReference type="eggNOG" id="COG0742">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_608108_0_0_5"/>
    <dbReference type="OMA" id="DHRFLLD"/>
    <dbReference type="OrthoDB" id="285876at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="450" mass="51233" checksum="631E27CED5F4781C" modified="2005-10-11" version="1">MTNEELLSQISDYCRRVGMAESTFGRRAVNDGKLVQRLREGKRITIDTLNRIEAYIVSGSPDGAVAVRKPEPIERRDPRANFRFFENRQKYLLFVHTCSEKRVIADRVALEFGSIYPRPPALRVFDAGVGDGTVLARVMRSMHGRFPHMPFYIAGKELSLEDIKLTLDKLPDRLFEHPSTVIVLTNMNYAEAPWLTPRSSQAAARMVWREVPLRGASSGEFEVQIRDLWPFLEENWRASVSPVTGMPVYERPVVLVIYREDHQFLLDSTIPRAGRVEASFDLVIASQPYRARASLNFKARRIIAPLARSLRPGGRLIGIHSHGQDPGLEIVQAVWPGENPFAVSRHELLRAVKYELGSVGRGLNFNAYADHRSIFRYDMEALPNEVTGAIGTSTLFAAWNAAVYVAQVEDDRLTEMTQSGRYLDATREVLRRHNGLWFYDESYVISRRRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ32</accession>
    <name>Q3SQ32_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RHH_4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2355</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315573.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2355"/>
    <dbReference type="EnsemblBacteria" id="ABA05609">
        <property type="protein sequence ID" value="ABA05609"/>
        <property type="gene ID" value="Nwi_2355"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2355"/>
    <dbReference type="eggNOG" id="COG4321">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155738_3_0_5"/>
    <dbReference type="OMA" id="SEIDDHR"/>
    <dbReference type="OrthoDB" id="1837705at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3990.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027373">
        <property type="entry name" value="RHH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038268">
        <property type="entry name" value="RHH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13467">
        <property type="entry name" value="RHH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RHH_4" evidence="1">
        <location>
            <begin position="6"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="84" mass="9209" checksum="A63DDFF570367827" modified="2005-10-11" version="1">MKSPVVKRSIVVAGHKTSVSLEEAFWNGMKEISSHRDMTLSELVGEIDSNRQQGNLSSAIRLFVLDYFRSRAATSGPENSPSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ66</accession>
    <name>Q3SQ66_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Amidinotransferase</fullName>
            <ecNumber evidence="5">2.1.4.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2321</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the amidinotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.4.1" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2321"/>
    <dbReference type="EnsemblBacteria" id="ABA05575">
        <property type="protein sequence ID" value="ABA05575"/>
        <property type="gene ID" value="Nwi_2321"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2321"/>
    <dbReference type="eggNOG" id="COG1834">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047415_1_0_5"/>
    <dbReference type="OMA" id="SHNEWDP"/>
    <dbReference type="OrthoDB" id="592329at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015068">
        <property type="term" value="F:glycine amidinotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033195">
        <property type="entry name" value="AmidinoTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10488">
        <property type="entry name" value="PTHR10488"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <feature type="active site" description="Amidino-cysteine intermediate" evidence="3">
        <location>
            <position position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR633195-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="386" mass="44327" checksum="7D6A4B077965E29E" modified="2005-10-11" version="1">MMLDTSGHPSNREQDENRASEDSPSRQACPVNSHNEWDPLEEVIVGRLEGATIPSNHPTVTSNIPGLAAKKVHALVAGMRFPKIMSEPAQRELDGFIKILESFGIKVRRPDAIDWKRRFGTPNWRARGFINACPRDSMLVIGDEIIETPMAWPTRYFETHSYRTLLKEYFRAGARWTSAPKPELRKELFDDAYRIPKEGDPIGYVLTEWEPVFDAADFMRCGRDLFVQKSNVTNAMGIEWLRRHLSDRGYRIHEIESRCRTPMHIDTTFVPLAPGKVLVNPEYLDVSRLPPILKDWEILVAPEPNPIEDRLLKITSMCGKWLNMNVLTIDEKRVVVERHHTSMIKALEKWGFEPVPCDFLHYGAFGGAFHCATLDIRRRGSLESYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQA6</accession>
    <name>Q3SQA6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2281</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315502.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2281"/>
    <dbReference type="EnsemblBacteria" id="ABA05535">
        <property type="protein sequence ID" value="ABA05535"/>
        <property type="gene ID" value="Nwi_2281"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2281"/>
    <dbReference type="eggNOG" id="COG3266">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132026_1_0_5"/>
    <dbReference type="OMA" id="AERFQDM"/>
    <dbReference type="OrthoDB" id="2050510at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045502">
        <property type="entry name" value="DUF6489"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20099">
        <property type="entry name" value="DUF6489"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="83" mass="9615" checksum="597DFA1D1A0A1E3D" modified="2005-10-11" version="1">MKMNIEIECTPLEARQFLGLPDVQPMQNAVMDQLQQKMAENLDKFSPEAIMKDWFDPKMAERFQDMFVNMSGLGTGRTTKDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQE5</accession>
    <name>Q3SQE5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Bcr/CflA family efflux transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_2242</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell inner membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Bcr/CmlA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2242"/>
    <dbReference type="EnsemblBacteria" id="ABA05496">
        <property type="protein sequence ID" value="ABA05496"/>
        <property type="gene ID" value="Nwi_2242"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2242"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_47_0_5"/>
    <dbReference type="OMA" id="MFQFGWI"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004812">
        <property type="entry name" value="Efflux_drug-R_Bcr/CmlA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00710">
        <property type="entry name" value="efflux_Bcr_CflA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="72"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="158"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="239"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="272"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="302"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="329"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="367"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="392"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="33"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="430" mass="45724" checksum="8AF5A73E2E8AEBF2" modified="2005-10-11" version="1">MAAYRSFRDTSMTDADMDGWVSRAHQPRGFPEFVALVACIMALNPIATDMMLPAMPDVRDAYAIVHPNHVQAVISVYLAGFGAGQFIMGPLSDRFGRRPILIGGMLLYSAAGLLSVVASSFETMLLARVLQGIGSSAARVIATSIVRDCYSGRRMASVMSLAMMIFISVPVLAPSLGQAILLFSEWRGIFVVLTAYGLLALLWCALRLPETLPPAGRKPLTAANVFDAFRQTVTNRQTIGYALAAGGVQSVLFAYVFCSQQIFTGIYGLGDYFPLAFAAIATGLAAAGFLNARIVGRFGMRVISHGALTGSVVIAALTLLAAWQGWLSFPVYMVFAIGTMFAFGLMFSNFTALAMEPQGHIAGTASSLFGSITTLIGSGVGAMIGQDFNGTMLRFTASAFLCTIAALVVVIVVERGRLFVPHNPAVPPPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQF6</accession>
    <name>Q3SQF6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF853 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2231</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315453.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2231"/>
    <dbReference type="EnsemblBacteria" id="ABA05485">
        <property type="protein sequence ID" value="ABA05485"/>
        <property type="gene ID" value="Nwi_2231"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2231"/>
    <dbReference type="eggNOG" id="COG0433">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028164_1_1_5"/>
    <dbReference type="OMA" id="TTEYGNI"/>
    <dbReference type="OrthoDB" id="600220at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033186">
        <property type="entry name" value="HerA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05872">
        <property type="entry name" value="DUF853"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF853" evidence="1">
        <location>
            <begin position="19"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="521" mass="56086" checksum="9BAF54F40528D79F" modified="2005-10-11" version="1">MTKTATGIGDTDQGIFIGKGEQPVWLTLGLANRHGLVTGATGTGKTVSLQVMAEGFSRAGVPVFVADIKGDLSGISEPGEARDFILDRARTMGLEFQPDRFSTIFWDVFGEQGHPVRATVSEMGPLLLSRMMDLNETQEGVLNIAFRVADEQGLMLLDMKDLRAILSFIAERAAELTTQYGNVSRQSVGAIQRRLLVLEHQGGDRFFGEPALDLNDFMRTDRDGRGMINILAADKLMQNPRLYATFLLWMLSELFERLPEVGDPPKPKLVFFFDEAHLLFNDAPKALMDKIEQVVRLIRSKGVGVYFVTQNPIDVPDKVLAQLGNRVQHALRAFTPRDQKAVAAAAQTFRPNPKLDTARVITELGKGEALVSCLEGNGTPAVVERVMVRPPSARIGPISPEERKALIAGSPVKGKYDTAIDSESAYELLQKRVEDTAAAADGSNGGVLGQIGSIVGSIFGTNTPRGRLSAGQVIARNVTRSVTNKVVGGIVADLGKSVGGSVGSSVGRALVRGALGGLLRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQJ5</accession>
    <name>CH602_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chaperonin GroEL 2</fullName>
            <ecNumber evidence="1">5.6.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">60 kDa chaperonin 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Chaperonin-60 2</fullName>
            <shortName evidence="1">Cpn60 2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">groEL2</name>
        <name evidence="1" type="synonym">groL2</name>
        <name type="ordered locus">Nwi_2192</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Together with its co-chaperonin GroES, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + a folded polypeptide = ADP + phosphate + an unfolded polypeptide.</text>
            <dbReference type="EC" id="5.6.1.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a cylinder of 14 subunits composed of two heptameric rings stacked back-to-back. Interacts with the co-chaperonin GroES.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the chaperonin (HSP60) family.</text>
    </comment>
    <dbReference type="EC" id="5.6.1.7" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315415.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SQJ5"/>
    <dbReference type="SMR" id="Q3SQJ5"/>
    <dbReference type="STRING" id="323098.Nwi_2192"/>
    <dbReference type="EnsemblBacteria" id="ABA05446">
        <property type="protein sequence ID" value="ABA05446"/>
        <property type="gene ID" value="Nwi_2192"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2192"/>
    <dbReference type="eggNOG" id="COG0459">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016503_3_0_5"/>
    <dbReference type="OMA" id="PYILINQ"/>
    <dbReference type="OrthoDB" id="265347at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03344">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00600">
        <property type="entry name" value="CH60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018370">
        <property type="entry name" value="Chaperonin_Cpn60_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001844">
        <property type="entry name" value="Cpn60/GroEL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00298">
        <property type="entry name" value="CHAPERONIN60"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="SSF48592"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="SSF52029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="SSF54849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02348">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00296">
        <property type="entry name" value="CHAPERONINS_CPN60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000256937" description="Chaperonin GroEL 2">
        <location>
            <begin position="1"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="30"/>
            <end position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="87"/>
            <end position="91"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="415"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="496"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00600"/>
        </source>
    </evidence>
    <sequence length="545" mass="57872" checksum="BF6663BCAA064129" modified="2005-10-11" version="1">MSAKDVKFGVDARDKMLRGVDILANAVKVTLGPKGRNVVLEKSFGAPRITKDGVTVAKEIELDDKFENMGAQMVREVASKSADAAGDGTTTATVLAQAIVKEGAKSVAAGMNPMDLKRGIDLAVEAVVADLSRNSKKVTSNEEIAQVGTISANGDSEIGKFLADAMKKVGNEGVITVEEAKSLETELDVVEGMQFDRGYISPYFVTNAEKMRVEMDDAYILINEKKLSSLNELLPLLEAVVQTGKPLVIVAEDVEGEALATLVVNRLRGGLKVAAVKAPGFGDRRKAMLQDIAILTGGQAISEDLGIKLENVTLQMLGRAKKVMIDKENTTIVNGAGKKADIEARVAQIKAQIEETTSDYDREKLQERLAKLAGGVAVIRVGGATEVEVKERKDRVDDAMHATRAAVEEGIVPGGGVALLRASEQLKRIKTQNDDQKTGVEIVRKALSAPARQIAINAGEDGSVIVGKILEKEQYSYGFDSQTGDYGNLISKGIIDPTKVVRTAIQNAASVASLLITTEAMVAELPKKNSPAPAMPGGGMGGMDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQK6</accession>
    <name>Q3SQK6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">zf-CHCC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2181</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2181"/>
    <dbReference type="EnsemblBacteria" id="ABA05435">
        <property type="protein sequence ID" value="ABA05435"/>
        <property type="gene ID" value="Nwi_2181"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2181"/>
    <dbReference type="eggNOG" id="COG4391">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2569770_0_0_5"/>
    <dbReference type="OMA" id="DEKICPY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019401">
        <property type="entry name" value="Znf_CHCC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10276">
        <property type="entry name" value="zf-CHCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="zf-CHCC" evidence="1">
        <location>
            <begin position="22"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="81" mass="8753" checksum="2901049FEF054856" modified="2005-10-11" version="1">MSDHVVPHFHNDSGVAVIEIGSREFMCVGANPPFDHPHVFLDLGDDGEIICPYCSTLYRFAADLGPGQSRPPGCVVRDKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQK7</accession>
    <name>Q3SQK7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2180"/>
    <dbReference type="EnsemblBacteria" id="ABA05434">
        <property type="protein sequence ID" value="ABA05434"/>
        <property type="gene ID" value="Nwi_2180"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2180"/>
    <dbReference type="eggNOG" id="ENOG5031HEF">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_756106_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="308"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="366" mass="38511" checksum="9F999B7936E7681F" modified="2005-10-11" version="1">MKNGIPPWACNEGAPRSGFSMSERRTGSAMTAAAICAAAFAVLAPRATDAGAVLVAQDDPVRLADLQLESVLRNDPALIGRNIEAALASQDPDLAGSFVDLAEARGISIPGDLTQRVTEAAEEENSTKGVAKRFFSGLVTGNAVDGASLSGTLAGDLCVFGDVRDIVREGSHLAKGEDTDRLLLGLAVAGVAVTGATFATVGSAAPARVGLTLVKDARKAGRLGEGLSRWAGRSAREMVDMRQLQKAFAAGSLAQPRGRRSPSRPRFAQRKPADWCGSPRMSAASARRLGRAVRSTFCGSLRGRKMWRAPPALQKPKADRPARSSRSWDEGRCCSPPVHSILRCGCSAPRWRCSVSCHRSGPRPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQL5</accession>
    <name>Q3SQL5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Carbonic anhydrases/acetyltransferases isoleucine patch superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2172</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315395.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2172"/>
    <dbReference type="EnsemblBacteria" id="ABA05426">
        <property type="protein sequence ID" value="ABA05426"/>
        <property type="gene ID" value="Nwi_2172"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2172"/>
    <dbReference type="eggNOG" id="COG0663">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064827_4_1_5"/>
    <dbReference type="OMA" id="YVTIGHN"/>
    <dbReference type="OrthoDB" id="1242595at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="176" mass="18424" checksum="7A812C5BD24BFB34" modified="2005-10-11" version="1">MATYELDGQGPDLPESGRYFIAETAAVIGRVRLRENASVWFGAVLRGDHEWIEVGAGSNVQDGCTCHTDEGFPLTIGSDCTIGHNVILHGCTIEDGALIGMGSIVMNGARVGRGCIVGAGALITEGKQFAEYSLIVGAPARVIRTLEPSEIETTGGGAKAYAANGQRFQKGLKRTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQL9</accession>
    <name>Q3SQL9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2168</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2168"/>
    <dbReference type="EnsemblBacteria" id="ABA05422">
        <property type="protein sequence ID" value="ABA05422"/>
        <property type="gene ID" value="Nwi_2168"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2168"/>
    <dbReference type="eggNOG" id="ENOG502Z876">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102943_0_0_5"/>
    <dbReference type="OMA" id="TWLANRD"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035438">
        <property type="term" value="F:cyclic-di-GMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009875">
        <property type="entry name" value="PilZ_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07238">
        <property type="entry name" value="PilZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PilZ" evidence="1">
        <location>
            <begin position="25"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="PilZ" evidence="1">
        <location>
            <begin position="128"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="214" mass="24114" checksum="8F51D3472D646333" modified="2005-10-11" version="1">MIPITGRPAGRFMALAQKISNLPVERRRFQRVRVHLLGRYMLPDRREFPCQVINMSPGGLAMLAPGIGHVGDRVIVYLDHIGRVEGRITRIIDSGFAMNISATPRKRDKLASQLTWLANRDILNLPEDRRHDRIMPRNPIAILTLEDGTRMTCRIIDLSMSGAAVSAEKTPPLQSRVALGKVLSRVVRNLEEGFALEFIHEQHPDTLEDDVTAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQP9</accession>
    <name>Q3SQP9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fec I like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2138</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Interacts transiently with the RNA polymerase catalytic core formed by RpoA, RpoB, RpoC and RpoZ (2 alpha, 1 beta, 1 beta' and 1 omega subunit) to form the RNA polymerase holoenzyme that can initiate transcription.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315361.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2138"/>
    <dbReference type="EnsemblBacteria" id="ABA05392">
        <property type="protein sequence ID" value="ABA05392"/>
        <property type="gene ID" value="Nwi_2138"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2138"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_22_0_5"/>
    <dbReference type="OMA" id="IRWMGAD"/>
    <dbReference type="OrthoDB" id="1335404at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="2">
        <location>
            <begin position="19"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="3">
        <location>
            <begin position="109"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="295" mass="33046" checksum="E799B56240DB84BE" modified="2005-10-11" version="1">MSDERSEPKRTWEASLDGLRPRLIRVAYRMLGSVTDAEDVVQEAFLRSLRVHRDDIRDAEAWLRRVVTRLSLNHLRSARRRRETYIGPWLPEPVIEPMENAFDDVTLPLLLALERLSPLERAAFLLHDVFGMSFDEIARTLDRTPAACRQLASRARAHVRVARPRFPVPKERGIEIAEAFFAASRSGDIQTLRSLLADDVTAFADGGGKTGATLEPVVGCDEVARLHEKLAALFAETPSRLIRYGLINGLPGFVTIEKATMLQTTALELENDKIVAIYVVQNPDKLQHLAGVAVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQR0</accession>
    <name>Q3SQR0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2126</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2126"/>
    <dbReference type="EnsemblBacteria" id="ABA05381">
        <property type="protein sequence ID" value="ABA05381"/>
        <property type="gene ID" value="Nwi_2126"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2126"/>
    <dbReference type="eggNOG" id="ENOG502ZCM1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118553_0_0_5"/>
    <dbReference type="OMA" id="EHVIQFV"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="199" mass="22225" checksum="23F70615C3FB0D51" modified="2005-10-11" version="1">MIRRSGRRASASTARCWSITPMRSASPANSGSDPANRQPFFLQPARPPRRALSFQEPDMADFFTHFSCLLDVGTPQNAARALEIYNRMMEENAAEDPLSEAFLLSIQPEHGGTQIWIRDDTTGDPQAVIEFVLRCAEEFGLTGRWGFQWANTCSRPRVNAFGGGAHVLDLGRRRTVAWIATNGWLDRTLSDERGKRGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQZ2</accession>
    <name>Q3SQZ2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Biopolymer transport protein ExbB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">Nwi_2040</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Involved in the TonB-dependent energy-dependent transport of various receptor-bound substrates. Protects ExbD from proteolytic degradation and functionally stabilizes TonB.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The accessory proteins ExbB and ExbD seem to form a complex with TonB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the exbB/tolQ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2040"/>
    <dbReference type="EnsemblBacteria" id="ABA05299">
        <property type="protein sequence ID" value="ABA05299"/>
        <property type="gene ID" value="Nwi_2040"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2040"/>
    <dbReference type="eggNOG" id="COG0811">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053325_0_0_5"/>
    <dbReference type="OMA" id="WHASAIA"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002898">
        <property type="entry name" value="MotA_ExbB_proton_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014164">
        <property type="entry name" value="TonB_ExbB_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01618">
        <property type="entry name" value="MotA_ExbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02797">
        <property type="entry name" value="exbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="306"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="352"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="MotA_ExbB" evidence="16">
        <location>
            <begin position="274"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="60"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="129"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ABA05299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="421" mass="42617" checksum="DD1B60F9BDBF78D3" modified="2005-10-11" version="1">MPAGSGPPEHASRWRRSQRKIIPMMRNIRTIAAGELPARIARGLLAAAFILAILPGDADARDRRPHPQPDVTASQPAATPPASAADAAPSTPSAAAPAPSGSAPAGDTAATPVAPVASPGPDTPAPSSGETPSPASADGASGEGSASSDASAGASSDAAAPSDQSAPAAAPSAPEGSSSTLPQDLSPWGMFMQADIVVKAVMIGLAFASLVTWTVWLAKGIELAASRRRAQSCVRKLERADSLDAARSEIAEGWTCEGTVADLMGAAERELQRSGDLSSEGIKERLAIALSRIEAKAGRDIARGTGLLATIGATAPFVGLFGTVWGIMNAFIGISQTKTTNLAVVAPGIAEALLATALGLVAAIPAVIIYNVFARALSSYRALLSDASGEIIQHISRDLERQERGLGPSVVALSRGRAAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQZ8</accession>
    <name>Q3SQZ8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2034</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315277.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2034"/>
    <dbReference type="EnsemblBacteria" id="ABA05293">
        <property type="protein sequence ID" value="ABA05293"/>
        <property type="gene ID" value="Nwi_2034"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2034"/>
    <dbReference type="eggNOG" id="ENOG5032ZII">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132651_0_0_5"/>
    <dbReference type="OMA" id="ADWIQGE"/>
    <dbReference type="OrthoDB" id="2015471at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229156" evidence="1">
        <location>
            <begin position="26"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="154" mass="16420" checksum="AAB2A75B6F6CB9E6" modified="2005-10-11" version="1">MYKEISVPTKLFAALIILWSLAGSAAAGPDENAVRDLLHSTFDKPEAKLDVAPVVAVSGYAIAGWTQGETGGRALLRNRNGRWAIILCAGDEIRNAEALRQAGIAPDVANALAQALAKAEQTVPADRLAMFARFEGLVRMDEAGNHPPVHDRAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR21</accession>
    <name>Q3SR21_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cytochrome c domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_2011</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315256.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2011"/>
    <dbReference type="EnsemblBacteria" id="ABA05270">
        <property type="protein sequence ID" value="ABA05270"/>
        <property type="gene ID" value="Nwi_2011"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2011"/>
    <dbReference type="eggNOG" id="ENOG502ZTUQ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048264_0_0_5"/>
    <dbReference type="OMA" id="YTTSSDM"/>
    <dbReference type="OrthoDB" id="1592915at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229218" description="Cytochrome c domain-containing protein" evidence="6">
        <location>
            <begin position="20"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="7">
        <location>
            <begin position="29"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="88"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="141"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="218"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="277"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="321"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="341"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA05270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="425" mass="42498" checksum="998665DDCF2B4FF9" modified="2005-10-11" version="1">MSSRALSLATVMLVTVVFAAGGAGAKNLDAGKSAAQIFSGNCAACHKGARGLLRTVPPGSLPGFLRQHYTTGPDMAGMLSAYLISNGATDQRRRVEPPKSHPGDLRTEPRQTERQGRKPLPQATQPPEAAAPQNEAQGSRDRRTPANKRKPGKQSPEEAAKAGAAGQGASPSDAAAKPETAKTEPSGATPPKENEAVKEEAAQGKTAKGAASNAGPAKEDAAEDKAAKDEAPKQESAKDEADGKQSASGRAANPKAVDSETPKPDSASSASDKPGVGKDGTADKPETEKSNAAGSDKPDATPADAATSGEPKTVPLRADPVPAVTPAPKPDPAPSGNDKPDIGKDGAEGKPGVDKSEAGKSGAAGSGKPDSIPADAPTSGEPKAAPLRADPIPAVTPAPKAGEGNTPPSTEPAASSPTPPPAPDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR92</accession>
    <name>Q3SR92_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">CRISPR-associated protein, Csd1 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1939</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315192.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1939"/>
    <dbReference type="EnsemblBacteria" id="ABA05199">
        <property type="protein sequence ID" value="ABA05199"/>
        <property type="gene ID" value="Nwi_1939"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1939"/>
    <dbReference type="eggNOG" id="ENOG502Z7WH">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031037_0_0_5"/>
    <dbReference type="OMA" id="TIRDRFY"/>
    <dbReference type="OrthoDB" id="924061at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010144">
        <property type="entry name" value="CRISPR-assoc_prot_Csd1-typ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09709">
        <property type="entry name" value="Cas_Csd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01863">
        <property type="entry name" value="cas_Csd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="593" mass="65879" checksum="E8D00B5051B0D029" modified="2005-10-11" version="1">MSILASLVRAYDHLPDAPPFGYSMQNIGVIVSLNEDGSVANVIDWRDGEGKRKKPRPMLVPQPVKRTAGIAPNFLWDKTSYVLGVTSGDGRRTADEHAAFKERHERELAGSDDAGLNALLKFLRQWTPDQFLAPVWPDEMRDQNVGFVLESDRRENLWLHQRDAAKALWERISQEASGGAQICLVTGEANPVARLHPSIKGVWGGQTAGASIVSFNLDAFTSYGHEQGDNAPVSELAAAKYTTALNRFLERGSGHRIQIGDASTVFWADSADAEAAKIAESAFSQFFDPADDAAAGDAAETRKIAILLERIRKGEQIEKIDAGLAKDVRFYVLGLAPNAARISIRFYFEDDFSVLGRNYQRFVEDMRIEPAWRQPYPPLWRYLRETAVLGKGENVPPNLAGEWMRSILSGTNYPLTLLAAVLSRIRADGEINALRVGMLRALLVRNYRMKPENRNKRREAPVALDPENRNKGYLLGRLFAVYEQAQTAALGRNVNATIKDKFYGSASATPRRVFSLLDRNSANHLSKVGKERPGQRVNIEKTIGAIMDMMMPGEDPFPASLSAAEQALFGLGYYHQRGEFFRKRETVAAEEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRA6</accession>
    <name>Q3SRA6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Type-5 uracil-DNA glycosylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_1925</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the uracil-DNA glycosylase (UDG) superfamily. Type 5 (UDGb) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1925"/>
    <dbReference type="EnsemblBacteria" id="ABA05185">
        <property type="protein sequence ID" value="ABA05185"/>
        <property type="gene ID" value="Nwi_1925"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1925"/>
    <dbReference type="eggNOG" id="COG1573">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083279_0_0_5"/>
    <dbReference type="OMA" id="CVPPQNK"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033958">
        <property type="term" value="F:DNA-deoxyinosine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10031">
        <property type="entry name" value="UDG-F5_TTUDGB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044147">
        <property type="entry name" value="UdgB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00986">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="SSF52141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDG" evidence="11">
        <location>
            <begin position="49"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA05185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="225" mass="24366" checksum="C1752C455C809EA2" modified="2005-10-11" version="1">MTTTLRTETRRSGATPPEPGRDCPLCPRLVDYRLAVRTREPEWFNAPVPSFGEPDSAVLIVGLAPGVQGANRTGRPFTGDYAGDLLYATLLKYGFAAGRYQARPDDGLKLIDCRISNAVRCVPPQNKPLPAEINTCRAFLSATLAAMPRLQAVVALGRVAHDSAVKALGVRAAAAPFAHGAVHHIGAVTLYDSYHCSRYNTNTRVLTPEMFESVFAKVRADLTCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRB7</accession>
    <name>Q3SRB7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA topoisomerase 4 subunit A</fullName>
            <ecNumber evidence="5">5.6.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Topoisomerase IV subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">parC</name>
        <name evidence="7" type="ordered locus">Nwi_1914</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterotetramer composed of ParC and ParE.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the type II topoisomerase GyrA/ParC subunit family. ParC type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315170.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1914"/>
    <dbReference type="EnsemblBacteria" id="ABA05174">
        <property type="protein sequence ID" value="ABA05174"/>
        <property type="gene ID" value="Nwi_1914"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1914"/>
    <dbReference type="eggNOG" id="COG0188">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002977_4_1_5"/>
    <dbReference type="OMA" id="PRSNRID"/>
    <dbReference type="OrthoDB" id="217468at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019897">
        <property type="term" value="C:extrinsic component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00187">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.268.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.199.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00936">
        <property type="entry name" value="ParC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006691">
        <property type="entry name" value="GyrA/parC_rep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035516">
        <property type="entry name" value="Gyrase/topoIV_suA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013758">
        <property type="entry name" value="Topo_IIA_A/C_ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013757">
        <property type="entry name" value="Topo_IIA_A_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002205">
        <property type="entry name" value="Topo_IIA_dom_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005742">
        <property type="entry name" value="TopoIV_A_Gneg"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03989">
        <property type="entry name" value="DNA_gyraseA_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00521">
        <property type="entry name" value="DNA_topoisoIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00434">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101904">
        <property type="entry name" value="SSF101904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="SSF56719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 5 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="TOP4c" evidence="6">
        <location>
            <begin position="16"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="5">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="5">
        <location>
            <position position="47"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="5">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="5">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="5">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="749" mass="83332" checksum="3E09F328443F4785" modified="2005-10-11" version="1">MGKRLIPAEPAEIAEVQLREALEERYLAYALSTIMHRALPDARDGLKPVHRRILYGMRLLRLDPGSAFKKSAKIVGDVMGSFHPHGDQAIYDAMVRLAQDFASRYPLVDGQGNFGNIDGDNPAAYRYTEARMTDVARLLLDGIDEDAVEFRPNYDGQSREPVVLPGGFPNLLANGAQGIAVGMATAIPPHNAAELCDAALHLIDKPDAKSTSLLKWVKGPDFPTGGIIIDSKESIAEAYATGRGAFRTRAKWHREEGARGTWVIVVTEIPWLVQKSRLIEKIAELLNEKKLPLVGDIRDESAEDIRVVIEPKSRNVDPELVMESLFRLTELESRISLNLNVLIKGKIPKVVGLAECLREWLDHLRDVLLRRSAHRKAQIEHRLEVLGGYLIAYLNIDEVIRIIRTEDEPKPALIKAFKLTEVQAEAILNMRLRSLRKLEEMEIRTEDKDLRAELKGINAVLGSEARQWEKVGEQVRNVRDMFGPRTPLGKRRTVFADAPEHDLAALEESLVEREPVTVVVSDKGWVRTLKGHVEDLSNLSFKTDDKLGFAFFAETTSKLLLFATNGKFYSLDVAKLPGGRGHGEPIRLFIDLEQDAAIVSLFVHTGGRKFLIASHEGQGFVVNEDDCVSTTRKGKQVLNVEMPNEAKAITTVAGDQVAVIGTNHKMVVFPLDQVPEMARGRGVRLQKYKDAGLSDVAVFNSKIGLTWKDSAGREQSLSWKDLSDWRGNRADAGRLAHGLPKSNKFGRAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRC7</accession>
    <name>Q3SRC7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glutamine synthetase</fullName>
            <ecNumber evidence="9">6.3.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_1904</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + L-glutamate + NH4(+) = ADP + H(+) + L-glutamine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:16169"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Oligomer of 12 subunits arranged in the form of two hexagons.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Oligomer of 12 subunits arranged in the form of two hexameric ring.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the glutamine synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.2" evidence="9"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315160.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1904"/>
    <dbReference type="EnsemblBacteria" id="ABA05164">
        <property type="protein sequence ID" value="ABA05164"/>
        <property type="gene ID" value="Nwi_1904"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1904"/>
    <dbReference type="eggNOG" id="COG0174">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017290_1_2_5"/>
    <dbReference type="OMA" id="PHPHEFE"/>
    <dbReference type="OrthoDB" id="416474at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamate-ammonia ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009399">
        <property type="term" value="P:nitrogen fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_b-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027303">
        <property type="entry name" value="Gln_synth_gly_rich_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004809">
        <property type="entry name" value="Gln_synth_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001637">
        <property type="entry name" value="Gln_synth_I_adenylation_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027302">
        <property type="entry name" value="Gln_synth_N_conserv_site"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03951">
        <property type="entry name" value="Gln-synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="SSF54368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00653">
        <property type="entry name" value="GlnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00180">
        <property type="entry name" value="GLNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00182">
        <property type="entry name" value="GLNA_ADENYLATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00181">
        <property type="entry name" value="GLNA_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0535">Nitrogen fixation</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gln-synt_C" evidence="10">
        <location>
            <begin position="103"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-AMP-tyrosine" evidence="6">
        <location>
            <position position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604809-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA05164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="469" mass="52590" checksum="73D7345C44D2611E" modified="2005-10-11" version="1">MKTANDVLKSIKDNDVKYVDLRFTDPRGKWQHVTFDISMIDEEFFAEGTMFDGSSIAGWKVINESDMTLMPDPATATIDPFFAETTMVLTCDILEPSTGEPYNRDPRGIAKKAEAMVKSMGIGDTVYVGPEAEFFVFDDVRFQTTPYNTGFKLDSSELPTNSDTEYEGGNLGHRIRTKGGYFPVPPQDSIQDMRSEMLGAMARMGVKVEKHHHEVASAQHELGMKFDTLTYMADQMQVYKYCIHQVAHIYGKTATFMPKPVYGDNGSGMHVHQSIWKDGKPTFAGSKYADLSETCLYYIGGIIKHAKAINAFTNPSTNSYKRLVPGYEAPVLLAYSARNRSASCRIPYTTSPKAKRVEVRFPDPMANPYLAFAAMLMAGLDGIKNKIDPGPAMDKDLYDLPKEELKQIPTVCGSLREALESLDKDRAFLKAGGVFDDDFINAFIELKMIEVERFEMTPHPVEFDMYYSL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRD2</accession>
    <name>CLPP_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent Clp protease proteolytic subunit</fullName>
            <ecNumber evidence="1">3.4.21.92</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Endopeptidase Clp</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">clpP</name>
        <name type="ordered locus">Nwi_1899</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec, and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).</text>
            <dbReference type="EC" id="3.4.21.92"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Fourteen ClpP subunits assemble into 2 heptameric rings which stack back to back to give a disk-like structure with a central cavity, resembling the structure of eukaryotic proteasomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S14 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.92" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315155.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRD2"/>
    <dbReference type="SMR" id="Q3SRD2"/>
    <dbReference type="STRING" id="323098.Nwi_1899"/>
    <dbReference type="MEROPS" id="S14.001"/>
    <dbReference type="PRIDE" id="Q3SRD2"/>
    <dbReference type="EnsemblBacteria" id="ABA05159">
        <property type="protein sequence ID" value="ABA05159"/>
        <property type="gene ID" value="Nwi_1899"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1899"/>
    <dbReference type="eggNOG" id="COG0740">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058707_3_2_5"/>
    <dbReference type="OMA" id="RDYWMKA"/>
    <dbReference type="OrthoDB" id="1728970at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07017">
        <property type="entry name" value="S14_ClpP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00444">
        <property type="entry name" value="ClpP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001907">
        <property type="entry name" value="ClpP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023562">
        <property type="entry name" value="ClpP/TepA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033135">
        <property type="entry name" value="ClpP_His_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10381">
        <property type="entry name" value="PTHR10381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00574">
        <property type="entry name" value="CLP_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00127">
        <property type="entry name" value="CLPPROTEASEP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00382">
        <property type="entry name" value="CLP_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0720">Serine protease</keyword>
    <feature type="chain" id="PRO_0000226451" description="ATP-dependent Clp protease proteolytic subunit">
        <location>
            <begin position="1"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00444"/>
        </source>
    </evidence>
    <sequence length="212" mass="23475" checksum="3089C6363E4EFF7E" modified="2005-10-11" version="1">MRDPVETYMNLVPMVVEQTNRGERAYDIFSRLLKERIIFVTGGVEDNMSMLVVAQLLFLEAENPKKEISMYINSPGGVVTSGLAIYDTMQFIRPPVATLCTGQAASMGSLLLAAGEKDMRHALPNARIMVHQPSGGFQGQATDIMLHAQEILSLKKRLNEIYVKHTGQTYQAIEDALERDKFLTADMARDFGIVDKVIDKRAEDPAAPKASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRG2</accession>
    <name>Q3SRG2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1869</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315125.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1869"/>
    <dbReference type="EnsemblBacteria" id="ABA05129">
        <property type="protein sequence ID" value="ABA05129"/>
        <property type="gene ID" value="Nwi_1869"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1869"/>
    <dbReference type="eggNOG" id="ENOG50301Y0">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147855_1_0_5"/>
    <dbReference type="OMA" id="CCAICAV"/>
    <dbReference type="OrthoDB" id="2046489at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021333">
        <property type="entry name" value="DUF2946"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11162">
        <property type="entry name" value="DUF2946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229147" evidence="1">
        <location>
            <begin position="26"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="121" mass="12667" checksum="DC64E923806FBC2A" modified="2005-10-11" version="1">MRRRLGRLLPIVMLAMLVQIFAPIAACRALSDTVGDPAAGVICTHAADDSVSQQADHQEASRACCTLCCVGASATPTADPQASIVDVERDTKAVRWRDLTFILPPLAVGSNAQARGPPGIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="127" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRH9</accession>
    <name>Q3SRH9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Site-2 protease, Metallo peptidase, MEROPS family M50B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_1852</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M50B family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315108.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1852"/>
    <dbReference type="EnsemblBacteria" id="ABA05112">
        <property type="protein sequence ID" value="ABA05112"/>
        <property type="gene ID" value="Nwi_1852"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1852"/>
    <dbReference type="eggNOG" id="COG0750">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025778_1_0_5"/>
    <dbReference type="OMA" id="QYMVGFG"/>
    <dbReference type="OrthoDB" id="1395197at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004387">
        <property type="entry name" value="Pept_M50_Zn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42837">
        <property type="entry name" value="PTHR42837"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="120"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="304"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="356"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="10">
        <location>
            <begin position="139"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA05112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="383" mass="40784" checksum="4453F3D879979E28" modified="2005-10-11" version="1">MADFFLNSFSTLSSGLIGYILPFLFVLTIVVFFHELGHFLVARWAGVKVLTFSVGFGPELAGFNDRHGTRWKLSAIPLGGYVKFFGDDSEASTPSSSTLASMTAEERGSSFHHKSVGRRAAIVAAGPIANFILAIVIFASLFMFLGKPSTTARVDGVQAGSAAEAAGFKAGDIVTAINGGRIDSFSDMQRIVGTKAGETLTFAVKRGDSIVDLKGVPELKEIKDRFGNTHRIGVLGITRATSPGDVTTEYVNPVTALWMGVEETWFVIDRTMAYIGGIFSGREAADQVGGPLRIAQISGQVATIGPAALIHLAAVLSVSIGLLNLFPVPLLDGGHLLFYAAEAVRGRPISERAQEVGFRIGLGLVLMLMVFATYNDILHLARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRI0</accession>
    <name>Q3SRI0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Outer membrane protein assembly factor BamA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">bamA</name>
        <name evidence="8" type="ordered locus">Nwi_1851</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the Bam complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the BamA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315107.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1851"/>
    <dbReference type="EnsemblBacteria" id="ABA05111">
        <property type="protein sequence ID" value="ABA05111"/>
        <property type="gene ID" value="Nwi_1851"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1851"/>
    <dbReference type="eggNOG" id="COG4775">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007664_1_2_5"/>
    <dbReference type="OMA" id="IFMPAFG"/>
    <dbReference type="OrthoDB" id="258122at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043165">
        <property type="term" value="P:Gram-negative-bacterium-type cell outer membrane assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051205">
        <property type="term" value="P:protein insertion into membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01430">
        <property type="entry name" value="OM_assembly_BamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000184">
        <property type="entry name" value="Bac_surfAg_D15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010827">
        <property type="entry name" value="BamA/TamA_POTRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039910">
        <property type="entry name" value="D15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023707">
        <property type="entry name" value="OM_assembly_BamA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034746">
        <property type="entry name" value="POTRA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815">
        <property type="entry name" value="PTHR12815"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815:SF23">
        <property type="entry name" value="PTHR12815:SF23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01103">
        <property type="entry name" value="Omp85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07244">
        <property type="entry name" value="POTRA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006076">
        <property type="entry name" value="OM_assembly_OMP85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03303">
        <property type="entry name" value="OM_YaeT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51779">
        <property type="entry name" value="POTRA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 6" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="domain" description="POTRA" evidence="7">
        <location>
            <begin position="107"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="POTRA" evidence="7">
        <location>
            <begin position="360"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA05111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="843" mass="93218" checksum="ACC814C085FC8399" modified="2005-10-11" version="1">MNVGMRVLRGILLSALIAIAVPAAVAVTVPLISSPAAAQTLSSISVQGNRRVELETIRSYFKPDASGRLDQASIDDALKALIETGLFQDVRIDRAGSGLVVSVIENPVIGRVAFEGNKKVKDDQLKTEIQSKPRGTFSRPMVQSDVQRIVEIYRRSGRYDVRVTPEFIEQPNNRVDLIFSIVEGDKTGVASIEFIGNQAFSSYRLKDVIKTHESNFLSFLGGGDVYDPDRVEADRDLLRRFYLKNGYADVQIIAALTEYDPGRHGFLVTFKIEEGQQYRVGSVAIESSIPTFDGNSLRSFSHVHVGSLYNAEALEKSVEEMQVEASRRGYAFAVVRPRGDRNFDAHTVSIVFAIDEGPRTYIERINIRGNTRTRDYVIRREFDLSEGDAYNRSLVDRAERRLKNLDFFKTVKITTEPGSSSDRVILDVEVEDKSTGDFSVSGGFSTTQGALAEVSVSERNLLGRGLYAKASVQYGQWSRGYSLSFVEPFLLDYRVALGLDLYQREQLSNQWISYNMKTLGFSPRLGLGLREDLTLQLRYSIFQQEIGLPSALRNCNNSLTNGLPYNPSPAFMNAMGVWDGVNMGCYSDGEASLPIRQELERGRTLTSMLGYTLTYNTLDNNKNPTAGLLIDFRQDFAGIGGDVKYLKTALDAKYYQPLVSDIVGLIHLQGGILNNVGAYAGQNGIRMLDNFQMGPNLVRGFAPYGIGPRDVNYLSTMDALGGTKYWGASVELQMPFWFMPKEVGLKGAVYADAGSLWGYQGPTSYALTNEFNAPGCSPSTSNANPGTCAGLQYDDTNVIRSSVGVGLIWQSPFGPLRFDYAVPITKGRFDRVQEFRFGGGTTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRL0</accession>
    <name>Q3SRL0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADPH-dependent FMN reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1820</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315077.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1820"/>
    <dbReference type="EnsemblBacteria" id="ABA05081">
        <property type="protein sequence ID" value="ABA05081"/>
        <property type="gene ID" value="Nwi_1820"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1820"/>
    <dbReference type="eggNOG" id="COG0431">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055322_4_2_5"/>
    <dbReference type="OMA" id="PADWTAF"/>
    <dbReference type="OrthoDB" id="1818437at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN_red" evidence="1">
        <location>
            <begin position="8"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="185" mass="20010" checksum="A07095BCDC0C33D4" modified="2005-10-11" version="1">MASQLNTAVIVGSLRKESFSLKLARALTALAPPSLQLDVVTLNGLSLYNQDLDATPPADWVDFRDRVRKADAVLFVTPEYNRSVPGVLKNAIDVGSRPYGQSVFNGKPGGVISNSPGALGGFGANHHLRQSLVFLNVSVLQQPEAYIGGIGDAFDEKGELVKTSLREFLEAYIKTFAAWVARHQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRN8</accession>
    <name>Q3SRN8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1792</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05053">
        <property type="protein sequence ID" value="ABA05053"/>
        <property type="gene ID" value="Nwi_1792"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1792"/>
    <dbReference type="eggNOG" id="ENOG5030X73">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144639_1_0_5"/>
    <dbReference type="OMA" id="GKWNGAS"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229082" evidence="1">
        <location>
            <begin position="26"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="116" mass="11889" checksum="D91EAF352F14B41B" modified="2005-10-11" version="1">MHRANTYAILFSGVLSAGLASPALAGNTYDGLWNVRIVTKTGNCDPVAQYPLTVINGKVTAGNSDITGSVGSQGYVKVSIRGAYANGQLNGNVGSGKWNGASGGIPCSGRWEASRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRN9</accession>
    <name>Q3SRN9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1791</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315048.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05052">
        <property type="protein sequence ID" value="ABA05052"/>
        <property type="gene ID" value="Nwi_1791"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1791"/>
    <dbReference type="eggNOG" id="ENOG502ZYPA">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_723266_0_0_5"/>
    <dbReference type="OMA" id="AAQGMEC"/>
    <dbReference type="OrthoDB" id="1419357at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025586">
        <property type="entry name" value="PcfJ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14284">
        <property type="entry name" value="PcfJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="381" mass="42381" checksum="9895DA0F7535AC8A" modified="2005-10-11" version="1">MKDDAQRGLLQWLAPEFAATGLLRDEPWVQACAREAESMYAVKPLAIAAVSMVVVEPFAIDYLRQAPVLVVAAAQGMECFRPSEREFVQQDWHAAIQDRPKLRDLLKSYGLIRPLRALKGQAFRAGPTEYQIIQNLCDIPPSPLAQAIPTAIRKQHNWLRALGEWSETMCRRQQSAKALFEWAVRAMGEHGASAAGGLLDQASAVADYAAFHIDGFNRRWSYERAYRAAIEWHDELARASSEKAYLLTHKIGWNDVIDYGDLPRRRIIDGYEIVALQSGAALHAEGADMHHCVASYAGNVIRGFSRIFSVRKDSQRVATFEISKSGFLLASGEFLQLPARKRSPYKLIQTKGPCNADVSPDVEKVIQAFILGLNEDTAAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="133" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRQ4</accession>
    <name>Q3SRQ4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Seryl-tRNA synthetase</fullName>
            <shortName evidence="8">SerRS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Seryl-tRNA(Ser/Sec) synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">serS</name>
        <name evidence="10" type="ordered locus">Nwi_1776</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + L-serine + tRNA(Sec) = AMP + diphosphate + H(+) + L-seryl-tRNA(Sec)</text>
            <dbReference type="Rhea" id="RHEA:42580"/>
            <dbReference type="Rhea" id="RHEA-COMP:9742"/>
            <dbReference type="Rhea" id="RHEA-COMP:10128"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + L-serine + tRNA(Ser) = AMP + diphosphate + H(+) + L-seryl-tRNA(Ser)</text>
            <dbReference type="Rhea" id="RHEA:12292"/>
            <dbReference type="Rhea" id="RHEA-COMP:9669"/>
            <dbReference type="Rhea" id="RHEA-COMP:9703"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer. The tRNA molecule binds across the dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.11" evidence="8"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315033.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1776"/>
    <dbReference type="EnsemblBacteria" id="ABA05037">
        <property type="protein sequence ID" value="ABA05037"/>
        <property type="gene ID" value="Nwi_1776"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1776"/>
    <dbReference type="eggNOG" id="COG0172">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023797_1_1_5"/>
    <dbReference type="OMA" id="SPCFRRE"/>
    <dbReference type="OrthoDB" id="353391at2"/>
    <dbReference type="UniPathway" id="UPA00906">
        <property type="reaction ID" value="UER00895"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016260">
        <property type="term" value="P:selenocysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097056">
        <property type="term" value="P:selenocysteinyl-tRNA(Sec) biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00770">
        <property type="entry name" value="SerRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00176">
        <property type="entry name" value="Ser_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015866">
        <property type="entry name" value="Ser-tRNA-synth_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042103">
        <property type="entry name" value="SerRS_1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033729">
        <property type="entry name" value="SerRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43697">
        <property type="entry name" value="PTHR43697"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02403">
        <property type="entry name" value="Seryl_tRNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001529">
        <property type="entry name" value="Ser-tRNA-synth_IIa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="SSF46589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00414">
        <property type="entry name" value="serS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="9">
        <location>
            <begin position="180"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Serine binding" evidence="8">
        <location>
            <begin position="299"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="330"/>
            <end position="332"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="353"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="420"/>
            <end position="423"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="455"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA05037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="497" mass="55467" checksum="BE1EC4DD75DAEC51" modified="2005-10-11" version="1">MHDIKAIRDNPQAFDAALKRRRLSDTDRVRFSSEEILKFDAERRTLIATVEQWKSTRNQISKEIGIAMREKDEVRVAALMEKVDRLKHDIISGGSNLIIEREVGLKNLLAEIPNLPLDEVPDGADEHGNVQHHIFGKKRDYAFTPKPHDDLGGALGDMDFEAAAKLSGARFVVLKRGLARLERAIGQFFLDVHTGEHGYTEVNPPLLVRDDAMFGTAQLPKFREDQFLARSPIGGNLLYDELDAETRESPEAIARYALAALAGRTIEEQGVLGWSNVAMPDFVKLREQLPVSTSWLIPTAEVPLTNLVRESILDEKELPLRFTALTPCFRAEAGAAGRDTRGMIRQHQFTKVELVSITTPETSKDEHERMLSCAEEVLRRLDLHYRVMTLCTGDMGFASQKTYDIEVWMPGQGEGGAYREISSCSVCGDFQARRMDARYRSSDGKPRFVHTLNGSGTAVGRALIAVMETYQQADGSIAVPDVLQPYMGGLKVIAKDM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-16" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRV3</accession>
    <name>GLYA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine hydroxymethyltransferase</fullName>
            <shortName evidence="1">SHMT</shortName>
            <shortName evidence="1">Serine methylase</shortName>
            <ecNumber evidence="1">2.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">glyA</name>
        <name type="ordered locus">Nwi_1727</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SHMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314984.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRV3"/>
    <dbReference type="SMR" id="Q3SRV3"/>
    <dbReference type="STRING" id="323098.Nwi_1727"/>
    <dbReference type="EnsemblBacteria" id="ABA04988">
        <property type="protein sequence ID" value="ABA04988"/>
        <property type="gene ID" value="Nwi_1727"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1727"/>
    <dbReference type="eggNOG" id="COG0112">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022477_2_1_5"/>
    <dbReference type="OMA" id="RCQHSEV"/>
    <dbReference type="OrthoDB" id="861782at2"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="UniPathway" id="UPA00288">
        <property type="reaction ID" value="UER01023"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="PTHR11680"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000234991" description="Serine hydroxymethyltransferase">
        <location>
            <begin position="1"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>(6S)-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="136"/>
            <end position="138"/>
        </location>
        <ligand>
            <name>(6S)-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
        </ligand>
    </feature>
    <feature type="site" description="Plays an important role in substrate specificity" evidence="1">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00051"/>
        </source>
    </evidence>
    <sequence length="433" mass="46223" checksum="0D5685D38F20B499" modified="2005-10-11" version="1">MDSSAKTASAPDPFFAGTLAEADPEIAAAITGELGRQRHEIELIASENIVSRAVLEAQGSVMTNKYAEGYPGARYYGGCEWVDVAETLAIERAKKLFGARFANVQPNSGSQMNQAVFLALLQPGDTFMGLDLAAGGHLTHGAPVNMSGKWFKVAHYTVRRDDHLIDMDEVARRAEEVKPKLIIAGGSAYSRPWDFKRFREIADSVGAYLMVDMAHFAGLVAGGVHASPVPHAHVTTTTTHKSLRGPRGGLILCNDEALAKKFNSAIFPGLQGGPLMHVIAAKAVALGEALRPDFKIYAKNVVENARALAESLRGHGFDIVSGGTDNHLMLVDLRPKGLKGNVSEKALVRAGLTCNKNGIPFDPEKPFVTSGLRLGTPATTTRGFGVSEFKQVGGLIAEVLTAIAQAEDGKAPLVEAAVKEKVKALTDRFPIYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRZ9</accession>
    <name>Q3SRZ9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">4-hydroxythreonine-4-phosphate dehydrogenase</fullName>
            <ecNumber evidence="4">1.1.1.262</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">4-(phosphohydroxy)-L-threonine dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pdxA</name>
        <name evidence="5" type="ordered locus">Nwi_1681</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the NAD(P)-dependent oxidation of 4-(phosphooxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphooxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>4-(phosphooxy)-L-threonine + NAD(+) = 3-amino-2-oxopropyl phosphate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:32275"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57279"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58452"/>
            <dbReference type="EC" id="1.1.1.262"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="4">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <text evidence="4">Binds 1 divalent metal cation per subunit. Can use ions such as Zn(2+), Mg(2+) or Co(2+).</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 4/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="4">The active site is located at the dimer interface.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PdxA family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.262" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314941.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1681"/>
    <dbReference type="EnsemblBacteria" id="ABA04942">
        <property type="protein sequence ID" value="ABA04942"/>
        <property type="gene ID" value="Nwi_1681"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1681"/>
    <dbReference type="eggNOG" id="COG1995">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040168_2_0_5"/>
    <dbReference type="OMA" id="TIGHEDR"/>
    <dbReference type="OrthoDB" id="1414545at2"/>
    <dbReference type="UniPathway" id="UPA00244">
        <property type="reaction ID" value="UER00312"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050570">
        <property type="term" value="F:4-hydroxythreonine-4-phosphate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008615">
        <property type="term" value="P:pyridoxine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00536">
        <property type="entry name" value="PdxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037510">
        <property type="entry name" value="PdxA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005255">
        <property type="entry name" value="PdxA_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30004">
        <property type="entry name" value="PTHR30004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04166">
        <property type="entry name" value="PdxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00557">
        <property type="entry name" value="pdxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0170">Cobalt</keyword>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="3 4 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0664">Pyridoxine biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="335" mass="34967" checksum="030F986CDDE65629" modified="2005-10-11" version="1">MVKPIALTLGEPAGIGPDIAIAAWLRRESDSVPPFYLLGDRAFIAARAKLLGHEIPLVDVGQGDVSRIFSEALPIAATGHTATAQPGDPDDNSAPAAIGSIRQAVSDVVAGHAGAVVTNPIAKSVLYRAGFAHPGHTEFLAELAAANANPPQPVMLLWSSDLAVVPVTIHLPLRDAISILSSRMIVDTVRIVVADLRARFGIRNPRIAVSGLNPHAGEDGSLGTEDQTIVAPAIATLRAEAIDVKGPLPADTMFHEAARRTYDCAICMYHDQALIPVKTLAFDRAVNVTLGLPFIRTSPDHGTAFDIAGTGRASPSSLIAALRLAARMAGPTPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS82</accession>
    <name>Q3SS82_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphoribosylglycinamide formyltransferase</fullName>
            <ecNumber evidence="2">2.1.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">5'-phosphoribosylglycinamide transformylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">GAR transformylase</fullName>
            <shortName evidence="2">GART</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">purN</name>
        <name evidence="4" type="ordered locus">Nwi_1598</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the transfer of a formyl group from 10-formyltetrahydrofolate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR) and tetrahydrofolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(6S)-10-formyltetrahydrofolate + N(1)-(5-phospho-beta-D-ribosyl)glycinamide = (6S)-5,6,7,8-tetrahydrofolate + H(+) + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide</text>
            <dbReference type="Rhea" id="RHEA:15053"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="EC" id="2.1.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GART family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.2" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314863.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1598"/>
    <dbReference type="EnsemblBacteria" id="ABA04859">
        <property type="protein sequence ID" value="ABA04859"/>
        <property type="gene ID" value="Nwi_1598"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1598"/>
    <dbReference type="eggNOG" id="COG0299">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038395_1_1_5"/>
    <dbReference type="OMA" id="TGITIHY"/>
    <dbReference type="OrthoDB" id="1815747at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00126"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004644">
        <property type="term" value="F:phosphoribosylglycinamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08645">
        <property type="entry name" value="FMT_core_GART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01930">
        <property type="entry name" value="PurN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004607">
        <property type="entry name" value="GART"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="SSF53328"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00639">
        <property type="entry name" value="PurN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Formyl_trans_N" evidence="3">
        <location>
            <begin position="4"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="5'-phosphoribosylglycinamide binding" evidence="2">
        <location>
            <begin position="13"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>(6S)-10-formyltetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>(6S)-10-formyltetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
        </ligand>
    </feature>
    <feature type="site" description="Raises pKa of active site His" evidence="2">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="217" mass="22998" checksum="9B87BA5874BD25C7" modified="2005-10-11" version="1">MKRRVAILISGRGSNMTALVEAAKAEDFPAEIAVVISNKPGAAGLARAQAAGVETLVIESKPFGKDRAAFEAELQSALDDRRIEFICLGGFMRLFTAEFVRGWHGRMLNIHPSLLPSFRGLDPHGQALRAGVKISGATVHFVVAETDAGPIVMQGAVAVRDDDTAETLAARVLDIEHRIYPDALRLVAGGGTRLDGDICKTSANASPDDSLISPSVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS91</accession>
    <name>Q3SS91_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribonuclease D</fullName>
            <shortName evidence="4">RNase D</shortName>
            <ecNumber evidence="4">3.1.13.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rnd</name>
        <name evidence="6" type="ordered locus">Nwi_1589</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Exonuclease involved in the 3' processing of various precursor tRNAs. Initiates hydrolysis at the 3'-terminus of an RNA molecule and releases 5'-mononucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>Exonucleolytic cleavage that removes extra residues from the 3'-terminus of tRNA to produce 5'-mononucleotides.</text>
            <dbReference type="EC" id="3.1.13.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RNase D family.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.5" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314856.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1589"/>
    <dbReference type="EnsemblBacteria" id="ABA04850">
        <property type="protein sequence ID" value="ABA04850"/>
        <property type="gene ID" value="Nwi_1589"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1589"/>
    <dbReference type="eggNOG" id="COG0349">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042387_0_0_5"/>
    <dbReference type="OMA" id="FMRVDTF"/>
    <dbReference type="OrthoDB" id="1462689at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033890">
        <property type="term" value="F:ribonuclease D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042780">
        <property type="term" value="P:tRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01899">
        <property type="entry name" value="RNase_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002121">
        <property type="entry name" value="HRDC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044876">
        <property type="entry name" value="HRDC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006292">
        <property type="entry name" value="RNase_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00570">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00474">
        <property type="entry name" value="35EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="SSF47819"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01388">
        <property type="entry name" value="rnd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50967">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="HRDC" evidence="5">
        <location>
            <begin position="208"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA04850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="382" mass="42632" checksum="89B30EB5A0344360" modified="2005-10-11" version="1">MDLITTSAELASVCARLARHPVITVDTEFLRETTYYPLLCVIQLASPDEAVVVDALAGGIDLKPFFALMSNESVLKVFHAARQDIEIIWHQAGLIPHPVFDTQVAAMVLGHGDSIAYDALVERITGHRPDKTHRFTDWSRRPLTAEQLEYAVADVTHLRDVFAALDADLSKRGRSDWVSEEMEVLTSPKTYDFHPERAWERLKSRVRKPKDLAVLMEVAAWREQEAQSRNIPRSRVLKDDAVGDIAIHAPSSPERLATLRSLPKGFERSQWGADIVAAVQRGLARDPRTLPRIEKPRNNSNGAATVELLKVLLRMTSERHAVASKVIATVDDLERIVADDEADVGALHGWRRELFGEAALALKHGRLALAIEKGRVVRVERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSC4</accession>
    <name>Q3SSC4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative phage protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1556</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314823.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1556"/>
    <dbReference type="EnsemblBacteria" id="ABA04817">
        <property type="protein sequence ID" value="ABA04817"/>
        <property type="gene ID" value="Nwi_1556"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1556"/>
    <dbReference type="eggNOG" id="ENOG5033670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165422_0_0_5"/>
    <dbReference type="OMA" id="AGEFYPC"/>
    <dbReference type="OrthoDB" id="2056576at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="91" mass="10368" checksum="C88EFD3B43090D1B" modified="2005-10-11" version="1">MGKYRKKPVEIEAFQWLGRSSEVGAPEWFRSALKHVGTPGSVWIDGDSAQIFTLEGMMAARRDDWIIRGVEGEIYPCKPDIFEATYDEVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSD6</accession>
    <name>Q3SSD6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phage portal protein, HK97</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1544</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314811.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1544"/>
    <dbReference type="EnsemblBacteria" id="ABA04805">
        <property type="protein sequence ID" value="ABA04805"/>
        <property type="gene ID" value="Nwi_1544"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1544"/>
    <dbReference type="eggNOG" id="COG4695">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033789_0_1_5"/>
    <dbReference type="OMA" id="YVHNSHI"/>
    <dbReference type="OrthoDB" id="389520at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006944">
        <property type="entry name" value="Phage/GTA_portal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006427">
        <property type="entry name" value="Portal_HK97"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04860">
        <property type="entry name" value="Phage_portal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01537">
        <property type="entry name" value="portal_HK97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="388"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="408"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="424" mass="46811" checksum="E355DEA4D4D43CCA" modified="2005-10-11" version="1">MGLWSRIFGSGASPEPRAAIQPAGGGVVITTSEQLEEALRTGNVTASGMTVTPDKAMRASAVYACVRIRSGVPANMPLHIKRRVDARTRDDASDHPLWSIFRRRPNRWQTPSQFKRMMTAHLLLRGNAYALIVRSRGAVKELIPLHPDRVKCEQLDSLALEYTYTRKDGRRVVLTQAEVFHLVGLTLDGVHGVSVITYARETIGLSLAQEDHGATVFKYGARPSTVLVHPKALGPEGIENLRSSLDEFRAGGDKEGRALILEEGIEVKPLAMTAEDAQWIESRKFSRSDIAMFFGVPPHMLGDTEKSTSWGTGIASQTQGFVTFCAEDDLTTWEETINRDLIVDNDNDIYARFNRASLVKGDIKMRWEAHVKALQWGVMSPNEVRALEDLNPREGGDIYYPPPNTAGGDDKPEKDDGDESSQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSF8</accession>
    <name>Q3SSF8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acriflavin resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1522</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314789.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1522"/>
    <dbReference type="EnsemblBacteria" id="ABA04783">
        <property type="protein sequence ID" value="ABA04783"/>
        <property type="gene ID" value="Nwi_1522"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1522"/>
    <dbReference type="eggNOG" id="COG0841">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002755_1_2_5"/>
    <dbReference type="OMA" id="FIIPCFY"/>
    <dbReference type="OrthoDB" id="145346at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2090.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027463">
        <property type="entry name" value="AcrB_DN_DC_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001036">
        <property type="entry name" value="Acrflvin-R"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32063">
        <property type="entry name" value="PTHR32063"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00873">
        <property type="entry name" value="ACR_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00702">
        <property type="entry name" value="ACRIFLAVINRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82714">
        <property type="entry name" value="SSF82714"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="334"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="360"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="387"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="431"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="463"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="529"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="859"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="883"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="909"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="954"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="987"/>
            <end position="1013"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1040" mass="110576" checksum="CD4B705D2FB9120A" modified="2005-10-11" version="1">MGVSEPFIRRPIATSLLGIALMIGGALGYWALPVSALPQVDFPTVQVRTQLPGASPDVVASLLTAPLERQLGQIPSLSSMTSTSSYGLSQISLQFNLDRDIDGATQDVQAAINAAAGILPRNLPYPPTYAKVNPADAPVMTLALTSDTVSMRAMSDIADTILTQRLSQISGVGRVSVLGGLKPAVRVQADLTRLAAYGISMEDLRTAIASANVSGPKGSLDGAQQAYTIAANDQVTTADAYRPIIIAYRNGSPVTIGDVARIVDGLENNRTGGWYNGTPAVIIDIQRQPGANVIEVVRQIREEIPKLQSAIPAGVDLTVVSDRTVTIRASVHDVQFTLILSIVLVTLVVLLFLRSLRATLIAGVALPLSLITSFGIMYFAGFSLDNLSLMALTIGTGFVVDDAIVMIENIVRHIENGESVMEAALRGAREIAFTVISLTVSLIAVFIPLLFMSGLVGRMFREFALTLTIAVVTSAVVSLTLTPMMCSRLLKRSEGGVSIPGVAAVNRSIDRLVAFYHLTLLWVLRHQQATLGVVLATIVATMLLYVVAPKGFLPLQDTASVIAVTEAGPDVSFAEMQRRQAEVAAVIKADPDVTGVVSVIGAASVNPTTNAGRLVMTLSPRNDRRDDVAAVIARLKARVASIPGMTVYFQPVQDIQISTRTSRSLYQYTLTGTDAAQVSEWTRKLVEELRRDPLFRDVSSEAQDAGLRAALNIDRARAGQLGVSLQAVNDTLNDAFAQRQISTVYGQSNQYRVVLEALPQYQRDPSILSKLFLPGVAGAQVPLSAVATLTRTTAPLAISHQAQFPASSLSFNLAPGEALGDAVKRVKTIETRIGMPSNIAGMFSGDAAEFSKSLAGQPWLILAALVTIYIVLGVLYESYIHPITILSTLPSAGIGAILALMLFGQDLSVIGLIGIILLMGIVKKNAIMMIDFALEAERRQGMSSTDAIVQACLLRFRPIMMTTLAALFGALPLAVESGTGSELRFPLGISIIGGLLLSQLLTLYTTPVIYLALDRLNKRIEQAVPTGGSGPPPGAIEGMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSG6</accession>
    <name>Q3SSG6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FAD assembly factor SdhE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1514</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SdhE FAD assembly factor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1514"/>
    <dbReference type="EnsemblBacteria" id="ABA04775">
        <property type="protein sequence ID" value="ABA04775"/>
        <property type="gene ID" value="Nwi_1514"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1514"/>
    <dbReference type="eggNOG" id="COG2938">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103054_1_0_5"/>
    <dbReference type="OMA" id="LFRCWHR"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005631">
        <property type="entry name" value="SDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036714">
        <property type="entry name" value="SDH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03937">
        <property type="entry name" value="Sdh5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109910">
        <property type="entry name" value="SSF109910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="122" mass="13896" checksum="EF70A18EC08139D2" modified="2005-10-11" version="1">MLTSIIPEAIWCRPGSSGLLAFAWNRRMTGSTRSSEGMDDRRKRLLYRCWHRGMREMDLILGRFVDEEIGSLTEAELKDLENLIEVPDPDLYAALSDGAPLASEYRTALFDRIKSFRRGQGA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-01-23" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSW4</accession>
    <name>RL23_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplW</name>
        <name type="ordered locus">Nwi_1366</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the early assembly proteins it binds 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome. Forms the main docking site for trigger factor binding to the ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Contacts protein L29, and trigger factor when it is bound to the ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314643.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSW4"/>
    <dbReference type="SMR" id="Q3SSW4"/>
    <dbReference type="STRING" id="323098.Nwi_1366"/>
    <dbReference type="EnsemblBacteria" id="ABA04627">
        <property type="protein sequence ID" value="ABA04627"/>
        <property type="gene ID" value="Nwi_1366"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1366"/>
    <dbReference type="eggNOG" id="COG0089">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037562_3_1_5"/>
    <dbReference type="OMA" id="FEVDHRA"/>
    <dbReference type="OrthoDB" id="1978865at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01369_B">
        <property type="entry name" value="Ribosomal_L23_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_L23/L15e_core_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001014">
        <property type="entry name" value="Ribosomal_L23/L25_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_L25/23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11620">
        <property type="entry name" value="PTHR11620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00276">
        <property type="entry name" value="Ribosomal_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="SSF54189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00050">
        <property type="entry name" value="RIBOSOMAL_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000272785" description="50S ribosomal protein L23">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01369"/>
        </source>
    </evidence>
    <sequence length="98" mass="10923" checksum="9AD203FD63724A23" modified="2005-10-11" version="1">MTIDANHYDVIVAPVITEKATMASEYNKVVFKVARKATKPQIKEAVEKLFDVKVKSVNTLVRKGKTKVFRGRLGSHSDNKRAVVTLEEGHRIDVTTGL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSW8</accession>
    <name>EFTU_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Elongation factor Tu</fullName>
            <shortName evidence="2">EF-Tu</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tuf</name>
        <name type="ordered locus">Nwi_1362</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314640.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSW8"/>
    <dbReference type="SMR" id="Q3SSW8"/>
    <dbReference type="STRING" id="323098.Nwi_1362"/>
    <dbReference type="PRIDE" id="Q3SSW8"/>
    <dbReference type="EnsemblBacteria" id="ABA04623">
        <property type="protein sequence ID" value="ABA04623"/>
        <property type="gene ID" value="Nwi_1362"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1362"/>
    <dbReference type="eggNOG" id="COG0050">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007265_0_0_5"/>
    <dbReference type="OMA" id="EGDKEWG"/>
    <dbReference type="OrthoDB" id="621774at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01884">
        <property type="entry name" value="EF_Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03697">
        <property type="entry name" value="EFTU_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_B">
        <property type="entry name" value="EF_Tu_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041709">
        <property type="entry name" value="EF-Tu_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033720">
        <property type="entry name" value="EFTU_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004541">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_bac/org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004160">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03143">
        <property type="entry name" value="GTP_EFTU_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="SSF50465"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00485">
        <property type="entry name" value="EF-Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0251">Elongation factor</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000015713" description="Elongation factor Tu">
        <location>
            <begin position="1"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="tr-type G">
        <location>
            <begin position="10"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="G1" evidence="1">
        <location>
            <begin position="19"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="G2" evidence="1">
        <location>
            <begin position="60"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="G3" evidence="1">
        <location>
            <begin position="81"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="G4" evidence="1">
        <location>
            <begin position="136"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="G5" evidence="1">
        <location>
            <begin position="174"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="19"/>
            <end position="26"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="81"/>
            <end position="85"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="136"/>
            <end position="139"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00118"/>
        </source>
    </evidence>
    <sequence length="396" mass="43180" checksum="149E73F45CDD765D" modified="2005-10-11" version="1">MAKAKFERNKPHCNIGTIGHVDHGKTSLTAAITKVLAEAGGATFTAYDQIDKAPEEKARGITISTSHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPAIVVFLNKCDMVDDPELLELVEMEVRELLSKYEFPGDDIPIIKGSALAALEDSDAKLGKEAVLELMKAVDAYIPQPERPVDQPFLMPVEDVFSISGRGTVVTGRVERGIVKVGEEIEIVGIRETQKTTVTGVEMFRKLLDQGQAGDNIGALLRGTKREDVERGQVLCKPGSVKPHTKFKAEAYILTKEEGGRHTPFFTNYRPQFYFRTTDVTGVVHLPAGTEMVMPGDNVAMEVHLIVPIAMEEKLRFAIREGGRTVGAGVVASIIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSY9</accession>
    <name>Q3SSY9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1341</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314620.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04602">
        <property type="protein sequence ID" value="ABA04602"/>
        <property type="gene ID" value="Nwi_1341"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1341"/>
    <dbReference type="HOGENOM" id="CLU_2618442_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="78" mass="8589" checksum="0194B2E960060F80" modified="2005-10-11" version="1">MRSADEGNVVPLRSNNGDIVDRMAADLIRFEATGTESDAIRSLMCTRRYSYSDIATFVDDALAVARQEIVTTEMARAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="126" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSZ5</accession>
    <name>Q3SSZ5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAD kinase</fullName>
            <ecNumber evidence="5">2.7.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">ATP-dependent NAD kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">nadK</name>
        <name evidence="6" type="ordered locus">Nwi_1335</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + NAD(+) = ADP + H(+) + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:18629"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the NAD kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.23" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1335"/>
    <dbReference type="EnsemblBacteria" id="ABA04596">
        <property type="protein sequence ID" value="ABA04596"/>
        <property type="gene ID" value="Nwi_1335"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1335"/>
    <dbReference type="eggNOG" id="COG0061">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073319_0_0_5"/>
    <dbReference type="OMA" id="NGIERMS"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00361">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="SSF111331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 5" id="KW-0520">NAD</keyword>
    <keyword evidence="3 5" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5 6" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="50"/>
            <end position="51"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="120"/>
            <end position="121"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="161"/>
            <end position="166"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA04596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="261" mass="29005" checksum="94C6148986EED7EF" modified="2005-10-11" version="1">MIMTSTRSYNRIAFVGGASPEARQALTELVKTYGNRDPEDADVLVALGGDGLMLQTLHRNMRSGKPIYGMHRGTVGFLMNEYSRHDLHARLAAATDTVIHPLLMRATDVHGTVHIHHAINEVSLFRQTHQAARLRILIDERERMAELIADGVMVATPAGSTAYNLSAQGPILPINAALLALTPISPFRPRRWRGALLPDTAIVTIETLEHEKRPVAAVADHDEARNVRRVEVSSDKTIAMRMLFDPGHSLEERILREQFGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST06</accession>
    <name>Q3ST06_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Patatin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1324</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314603.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04585">
        <property type="protein sequence ID" value="ABA04585"/>
        <property type="gene ID" value="Nwi_1324"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1324"/>
    <dbReference type="eggNOG" id="COG1752">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047251_2_0_5"/>
    <dbReference type="OMA" id="CSMPGLL"/>
    <dbReference type="OrthoDB" id="373926at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="SSF52151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="1 2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="3">
        <location>
            <begin position="30"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="2">
        <location>
            <begin position="61"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="2">
        <location>
            <begin position="177"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="348" mass="37153" checksum="53DDD6E8A024596B" modified="2005-10-11" version="1">MPEDMKGRDQNGSNGRKVGLDSVRRPVIGLALGGGAARGFAHIGILRSLIAHGIQPDVVVGTSIGAVVGGAYAAGRLDTMEEWARSLQPRSLFSYLDIRLNGSGLIGGAKLTARLENALGDIQIEELPLKFASVATEVRTGHEIWLTHGRLVDAMRASYALPGIFAPMLVGDRWLVDGALVNPVPVSTARALGAEVVIAANLSNDVFGRSTTIFSHGTQNGVQEEIVEPPPSRLGFSRFFSLERTVKREFFGGGNRPGVVSVMTDAFNIMQDRITRARLAGDPPDLLISPRVGQIGWFDFHRADDLIEHGTRAADRAIEAIVEAIEVLAPASAPDTIRPADAEDGTEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST11</accession>
    <name>Q3ST11_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1319</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314598.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04580">
        <property type="protein sequence ID" value="ABA04580"/>
        <property type="gene ID" value="Nwi_1319"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1319"/>
    <dbReference type="HOGENOM" id="CLU_125951_0_0_5"/>
    <dbReference type="OMA" id="MIRHEAG"/>
    <dbReference type="OrthoDB" id="2215785at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="156" mass="16947" checksum="FAD881531BB8EEBE" modified="2005-10-11" version="1">MRQAGRVARFWVAAFGVVVGLQSACIPAGSDEFRGGGAAIENSQFRLALLIKNEAGHARDGSTILVSEVSAEKAEVSEDTQASETSAEKAEVPADTQANEVPAAKEEVPAERERDHSARSLEEMLAWFLKGAALLTAMVLFYKGLKWWVLRNRQSE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-03-06" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST91</accession>
    <name>KUP_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable potassium transport system protein kup</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">kup</name>
        <name type="ordered locus">Nwi_1238</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transport of potassium into the cell.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HAK/KUP transporter (TC 2.A.72) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ST91"/>
    <dbReference type="STRING" id="323098.Nwi_1238"/>
    <dbReference type="EnsemblBacteria" id="ABA04500">
        <property type="protein sequence ID" value="ABA04500"/>
        <property type="gene ID" value="Nwi_1238"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1238"/>
    <dbReference type="eggNOG" id="COG3158">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008142_4_2_5"/>
    <dbReference type="OMA" id="AADQFEI"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01522">
        <property type="entry name" value="Kup"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003855">
        <property type="entry name" value="K+_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023051">
        <property type="entry name" value="K+_uptake_low_affin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30540">
        <property type="entry name" value="PTHR30540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02705">
        <property type="entry name" value="K_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0630">Potassium</keyword>
    <keyword id="KW-0633">Potassium transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000279803" description="Probable potassium transport system protein kup">
        <location>
            <begin position="1"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="309"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="366"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="396"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="423"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="448"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="651" mass="70619" checksum="F392CE07CB50BE5B" modified="2005-10-11" version="1">MRDSPGSKSSSERWHDTMAVSDPTAEGKDESSAKTSGFWALTLGSVGVVFGDIGTSPLYAFREAVDHAAQEGAVTPAIVLGVLSLILWSLFIVVTAKYVLLLLRADNNGEGGTLSLMALGQRALGRRSLLLLALGVVGASMFIGDSMITPAISVLSAVEGLKLAAPRLQDYVVPLTLVILVMLFAVQSRGTARVASAFAPVMALWFLTIAVLGVLHIHEDPHVLLAVNPWYAIHFLLNHGLLGLVIMGLVFLSVTGGEALYADLGHFGRKPIQVAWFCLVLPSLLLNYFGQGALILAHPEAIENTFYRLAPAPLILPLVILATAATVIASQAVITGAYSLIRQGVQLGLLPRFEVRYTSETHAGQIYLPRVNLLLLIGVLLLVLLFRTSSGLASAYGIAVSTTMVADGIMGFVVVWKLWNWHPAAAAALVVPLVVVDMMFFSANLLKLFDGAWVPLLFGIAMVVLIWTWRRGVAILIKKTRRTEVPLLDLIQSLEKRPPHIVKGTAVFLTSDPNFVPTALLHNLKHNKVLHEHNVILTIETAQTPRVGPAERVRMENISEKFSTVSLRFGFMESPNVPKALAIARKLGWQFDIMSTSFFVSRRSLKASAQSGMPAWQDKLFIALSRSANDAIDYFQIPTGRVVEVGTQVII</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST96</accession>
    <name>Q3ST96_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="14">ABC transporter, transmembrane region</fullName>
            <ecNumber evidence="14">3.6.3.27</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Nwi_1233</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in beta-(1--&gt;2)glucan export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.27" evidence="14"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314524.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1233"/>
    <dbReference type="EnsemblBacteria" id="ABA04495">
        <property type="protein sequence ID" value="ABA04495"/>
        <property type="gene ID" value="Nwi_1233"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1233"/>
    <dbReference type="eggNOG" id="COG5265">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_3_5"/>
    <dbReference type="OMA" id="VTEWRTH"/>
    <dbReference type="OrthoDB" id="643917at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="3 11 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="89"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="49"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="378"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="625"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="411"/>
            <end position="418"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABA04495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="652" mass="72349" checksum="4705A4DEEFAA9B6D" modified="2005-10-11" version="1">MADAAFPQATAPAKPSDDPTRNATLGRALLHLWPYMWPGDRADLKMRVAWSLVLLLAAKVATLVVPFTFKWAVDALGGTGSAPVQPDNWAMWLIVSPLAMTLAYGSARVLMALLTQWRDGTFAKVAMHAVRRLAYRTFVHMHELSLRFHLERKTGGLTRVLERGRNGIEEMVRLVILQLIPTAVEVGLLTAVLLWQFDWRYVLVTLLTVMVYMAFTYKATQWRIEIRRRMNDSDSDANTKAIDSLLNYETVKYFSAETREADRYDRSMERFETASVQTYTSLAVLNAGQAVIYTAGLTTVMIMCAIGVRNGTHTVGDFVMVNAMMIQLYQPLNLMGMVYREIKQAIIDIEKMFSVLTRRAEVQDRPGAQPLRVESGAVKFEDVLFAYDPDRPILKGLTFDVPAGKTVAIVGPSGAGKSTLSRLLFRLYDVSSGRILIDGQDIRDVTQESLRAAIGMVPQDTVLFNDTIRYNIRYGRWDATDEEVEAAARTAQIDGFIRMSPKGYETEVGERGLKLSGGEKQRVAIARTVLKAPPILVLDEATSALDSHTEHEIQEALERVSRNRTSLVIAHRLSTIVGADEIIVLDQGRIAERGTHAELLAAGGLYASMWNRQREAEEAREKLALIGDEEGAPNRAPPPVDDPLVAPTGAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STE2</accession>
    <name>Q3STE2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Two component transcriptional regulator, winged helix family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_1187</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314478.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1187"/>
    <dbReference type="EnsemblBacteria" id="ABA04449">
        <property type="protein sequence ID" value="ABA04449"/>
        <property type="gene ID" value="Nwi_1187"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1187"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_1_5"/>
    <dbReference type="OMA" id="SYPRRVW"/>
    <dbReference type="OrthoDB" id="1020672at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="2"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="5">
        <location>
            <begin position="124"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="3">
        <location>
            <begin position="124"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA04449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="223" mass="25040" checksum="8F26EA29E150327B" modified="2005-10-11" version="1">MRLLVVEDDPDLNRQLTTALTDAGYVVDRAFDGEEAHFLGDSEPYDAVVLDIGLPKMDGISVLEAWRRGGRTMPVLILTARDRWSDKVQGFDAGADDYVAKPFHLEEILARMRALLRRSTGHAHSELTCGPVTLDTRTGRVSVGGNPVKMTSHEYRLLAYLMHHIGRVVSRTELVEHLYDQDFDRDSNTIEVFVGRIRKKLDVDIIQTVRGLGYLLTPPADAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STH4</accession>
    <name>Q3STH4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1155</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314447.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1155"/>
    <dbReference type="EnsemblBacteria" id="ABA04417">
        <property type="protein sequence ID" value="ABA04417"/>
        <property type="gene ID" value="Nwi_1155"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1155"/>
    <dbReference type="eggNOG" id="ENOG5033FKT">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106193_1_0_5"/>
    <dbReference type="OMA" id="LKTEMAN"/>
    <dbReference type="OrthoDB" id="1788323at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="155" mass="17467" checksum="6F658D347BB33597" modified="2005-10-11" version="1">MISEAMAGASALKTAFDMAKALKDISDAAIRNGAVIELQEKILSAQEAQFALVKRVDDLEKQVASFEAKGSQMQRYQLKDYGAGSFAYELKESEARGEPIHRACAACYQQGHISILQFSHNTEGQDWYECKRCNNMHHFGTRVPRTYSRTKTDYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STV3</accession>
    <name>Q3STV3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative phosphoketolase</fullName>
            <ecNumber evidence="2">4.1.2.22</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1026</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.1.2.22" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04288">
        <property type="protein sequence ID" value="ABA04288"/>
        <property type="gene ID" value="Nwi_1026"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1026"/>
    <dbReference type="eggNOG" id="COG3957">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2955934_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047905">
        <property type="term" value="F:fructose-6-phosphate phosphoketolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005593">
        <property type="entry name" value="Xul5P/Fru6P_PKetolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018970">
        <property type="entry name" value="Xul5P/Fru6P_PKetolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31273">
        <property type="entry name" value="PTHR31273"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09364">
        <property type="entry name" value="XFP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XFP_N" evidence="1">
        <location>
            <begin position="2"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="59" mass="6639" checksum="853ADC32286F9D53" modified="2005-10-11" version="1">MLREPLSPRHIKPRLLGYWGTTPGLNFIYAHLNRAIRAFDLNMIYICGPGHSGPGMETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STY8</accession>
    <name>Q3STY8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_0991</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YkuD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314292.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0991"/>
    <dbReference type="EnsemblBacteria" id="ABA04253">
        <property type="protein sequence ID" value="ABA04253"/>
        <property type="gene ID" value="Nwi_0991"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0991"/>
    <dbReference type="eggNOG" id="COG2989">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3266">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020360_2_0_5"/>
    <dbReference type="OMA" id="AFNENRG"/>
    <dbReference type="OrthoDB" id="975147at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071972">
        <property type="term" value="F:peptidoglycan L,D-transpeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16913">
        <property type="entry name" value="YkuD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.101.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005490">
        <property type="entry name" value="LD_TPept_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045380">
        <property type="entry name" value="LD_TPept_scaffold_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036366">
        <property type="entry name" value="PGBDSf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038063">
        <property type="entry name" value="Transpep_catalytic_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20142">
        <property type="entry name" value="Scaffold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03734">
        <property type="entry name" value="YkuD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141523">
        <property type="entry name" value="SSF141523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="SSF47090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229246" evidence="5">
        <location>
            <begin position="33"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="domain" description="Scaffold" evidence="8">
        <location>
            <begin position="274"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="PG_binding_1" evidence="6">
        <location>
            <begin position="446"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="YkuD" evidence="7">
        <location>
            <begin position="523"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="48"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="112"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="160"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA04253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="811" mass="88152" checksum="24767083F9C3BBF1" modified="2005-10-11" version="1">MRDDPTSRTGFDRILTVLAATFLAVSTSAAFAQADTVKKSASELAIDAAIPSPEPANVPPPTAADFKAEGQQPAAQVVGESKPDQQKADNGETDSSVSDPKPADTTAVDTKPAGAAASNSDTPSYAPTPPDETASSDAEPSGVEPEPGSNAGTAETKPAKPKITDAKSDTEVGSKETGSKDVTAASPDGIKTGQETDQETGKATEATKDATATIRPDVTPRPEAGAAANSEQPGEPVKTVINVPPADQPTAEKLHGLLDANATKLFSRKAERAAVEKFYTARSYAPLWTEGGTLNTQAQGVIARLRDAAADGLNPSDYPVPDFAAASSPEQLAEADLKLTASMMDYARQAQSGRMHWTQVSADIQYPEHPVDPEQVLTNVTTAKDSSAALDSYNPPHKLFQALKRKLAELRKDSEGPVIEIAEGPGLVFRKNAVIEDPRVPQIRARLGVTQNPDDTRFDADVAAAVREFQARHHLKPDGILGNNTIRALNGPKKDHTIDTIIANMERWRWLPRELGAPSLGDAYAILNIPDYSLKVMQHGAPIWSTRVVVGKPGLHATPLLTETMKYITVNPTWNVPPSIIYKEYLPALQQDPTVLSRMGLKLERDRNGGIHISQPPGERNALGRIRFNFPNKFLVYQHDTPDKHLFAKEKRAFSHGCMRVQNPDQYAATLLNIVLPQEHYTPEKVRSMYGRNEFNINFPTPFPVHITYQTAFVDQAGKLQLRGDVYGRDAKMLTLLRNSNGRDLETVVAHAQPNYSRPRGNIPQQGMAFNENRGFGGGFDNGGPSFFERLFGVQTPPPQYDDRRRPIYRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUB4</accession>
    <name>Q3SUB4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AAA_15 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0863</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314172.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0863"/>
    <dbReference type="EnsemblBacteria" id="ABA04127">
        <property type="protein sequence ID" value="ABA04127"/>
        <property type="gene ID" value="Nwi_0863"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0863"/>
    <dbReference type="eggNOG" id="COG0419">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1239093_0_0_5"/>
    <dbReference type="OrthoDB" id="318943at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041685">
        <property type="entry name" value="AAA_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13175">
        <property type="entry name" value="AAA_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_15" evidence="2">
        <location>
            <begin position="4"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="223" mass="24317" checksum="6A67FA5A9759D422" modified="2005-10-11" version="1">MARIRIIDIRNFRCLKSFIWRPSGGINCLIGPGDAGKSSVLDAIDYCLGARRNLSFSDADFHGLDVSAPISISVTLGELDDGLKSMEVYGNYLRSFDAATGEIDDEPESGKETVLTLNLSVKSDLEPVWTLVSDRAEAQGQTRYLTWSDRNRLCPTRIGAFAENDLAWRRSSVLTKLSDEKAETSGAMAVAARQARAAFGDIAEQQLSARRPSPGHETGRTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUC7</accession>
    <name>Q3SUC7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Lytic transglycosylase, catalytic</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0850</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the virb1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0850"/>
    <dbReference type="CAZy" id="GH23">
        <property type="family name" value="Glycoside Hydrolase Family 23"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04114">
        <property type="protein sequence ID" value="ABA04114"/>
        <property type="gene ID" value="Nwi_0850"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0850"/>
    <dbReference type="eggNOG" id="COG0741">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077897_0_0_5"/>
    <dbReference type="OMA" id="NAGPQRY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008258">
        <property type="entry name" value="Transglycosylase_SLT_dom_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01464">
        <property type="entry name" value="SLT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="SLT" evidence="4">
        <location>
            <begin position="138"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="292"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="336" mass="35641" checksum="A206EE003BF801BB" modified="2005-10-11" version="1">MTQIGTFTKTKTGFSGRLRTLALDVERPSFRSLSAVIPLIDRIADHPSDPIARQAAVARSAGQGRPQGRRSLALDRSEHGGRLMASRNGGPCTALSSIIATLIACGVIVVAAPTASADPTPTNRGSTVAPSGPWAPHIAEAAKRFAIPERWIRAVMAVESHGDRTARSPKGAIGLMQIMPKTWDELRARHALGNDPWQPRANILAGAAYLREMHDRYGTVEAMLAAYNAGPGRYDKHLATGQALPAETIDYVAKITPTIDVTASVVRSIGRPLHPSWSRAPLFVARSIAAGEDDRDAGESPSGRPSNGLPITGLSALVPPSAGLFVRRPEDDGGQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUD2</accession>
    <name>Q3SUD2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH_17 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0845</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314156.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0845"/>
    <dbReference type="EnsemblBacteria" id="ABA04109">
        <property type="protein sequence ID" value="ABA04109"/>
        <property type="gene ID" value="Nwi_0845"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0845"/>
    <dbReference type="eggNOG" id="COG3311">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140176_9_0_5"/>
    <dbReference type="OMA" id="THEEVPL"/>
    <dbReference type="OrthoDB" id="2076478at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041657">
        <property type="entry name" value="HTH_17"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12728">
        <property type="entry name" value="HTH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_17" evidence="1">
        <location>
            <begin position="14"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="80" mass="8639" checksum="CE64097DB1EF8DE4" modified="2005-10-11" version="1">MPEKTEKTWPRFVRTPEAARLLGLSPRTLEKHRCDGTGPVYHKLGGRVVYTIADIEAWIDVCARQSTSESAAGQAAGAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUJ2</accession>
    <name>Q3SUJ2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Antitoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0784</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Antitoxin component of a type II toxin-antitoxin (TA) system.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the phD/YefM antitoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0784"/>
    <dbReference type="EnsemblBacteria" id="ABA04049">
        <property type="protein sequence ID" value="ABA04049"/>
        <property type="gene ID" value="Nwi_0784"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0784"/>
    <dbReference type="eggNOG" id="COG2161">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2524164_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006442">
        <property type="entry name" value="Antitoxin_Phd/YefM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036165">
        <property type="entry name" value="YefM-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02604">
        <property type="entry name" value="PhdYeFM_antitox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143120">
        <property type="entry name" value="SSF143120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="59"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="88" mass="9759" checksum="209434CE14CF7AA0" modified="2005-10-11" version="1">MSNLKRGMSVKSVSAREAKYGFGKLIDTARAEPVTVEKHGRAVVVVLAVEEYERLKAIESEQSAKAPRSGEKKRGGAEWLNRRRKTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUR9</accession>
    <name>Q3SUR9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Invasion associated locus B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0706</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314028.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0706"/>
    <dbReference type="EnsemblBacteria" id="ABA03972">
        <property type="protein sequence ID" value="ABA03972"/>
        <property type="gene ID" value="Nwi_0706"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0706"/>
    <dbReference type="eggNOG" id="COG5342">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096085_3_0_5"/>
    <dbReference type="OMA" id="TETYGLW"/>
    <dbReference type="OrthoDB" id="2066387at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038696">
        <property type="entry name" value="IalB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010642">
        <property type="entry name" value="Invasion_prot_B"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06776">
        <property type="entry name" value="IalB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229231" evidence="1">
        <location>
            <begin position="28"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="186" mass="19513" checksum="F272CD530D85FC1A" modified="2005-10-11" version="1">MSARFMRTCLLTLAAAITTVAMTAAHAQQPATPAASTLPNGAASVSETYGDWTVDCRLVDRRKQCLLSQVQGNKETGRRVYAIELTPPAGGKIEGTILMPFGLNLDAGAVLKLDDKDLGKGLRFSTCVPQGCLLPVSFPATATDAMRKGTKLVVASLNLSSNEPVTFSVSLNGFAAALARVTELAK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUS4</accession>
    <name>Y701_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PKHD-type hydroxylase Nwi_0701</fullName>
            <ecNumber evidence="1">1.14.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">Nwi_0701</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="1">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="1.14.11.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314023.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SUS4"/>
    <dbReference type="SMR" id="Q3SUS4"/>
    <dbReference type="STRING" id="323098.Nwi_0701"/>
    <dbReference type="EnsemblBacteria" id="ABA03967">
        <property type="protein sequence ID" value="ABA03967"/>
        <property type="gene ID" value="Nwi_0701"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0701"/>
    <dbReference type="eggNOG" id="COG3128">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106663_0_0_5"/>
    <dbReference type="OMA" id="VGCYHNL"/>
    <dbReference type="OrthoDB" id="1139586at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016706">
        <property type="term" value="F:2-oxoglutarate-dependent dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00657">
        <property type="entry name" value="Hydroxyl_YbiX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041097">
        <property type="entry name" value="PKHD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023550">
        <property type="entry name" value="PKHD_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41536">
        <property type="entry name" value="PTHR41536"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18331">
        <property type="entry name" value="PKHD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0223">Dioxygenase</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0847">Vitamin C</keyword>
    <feature type="chain" id="PRO_1000061724" description="PKHD-type hydroxylase Nwi_0701">
        <location>
            <begin position="1"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="1">
        <location>
            <begin position="78"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>2-oxoglutarate</name>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00657"/>
        </source>
    </evidence>
    <sequence length="226" mass="25142" checksum="30A979144322B9BE" modified="2005-10-11" version="1">MIQVISDVLTPDELKRFRELLGQAQWQDGRATAGHVAVRAKANEQLSHEDSLGQQLSEFLLERLGKISHFIAAALPLKVLPPRFNRYTGGGSYGDHIDNAIFSVPGAGVRIRGDLSATLFLSEPGDYDGGELIIQGEFARHQFKLPAGQMILYPASTFHQVTPVTRGARLAAFFWTQSLVREHSRRALLFELDNTIQALAQDNPEQPAVARLTGLYHNLLREWSET</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUS5</accession>
    <name>Q3SUS5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">TonB-dependent receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_0700</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the TonB-dependent receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0700"/>
    <dbReference type="EnsemblBacteria" id="ABA03966">
        <property type="protein sequence ID" value="ABA03966"/>
        <property type="gene ID" value="Nwi_0700"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0700"/>
    <dbReference type="eggNOG" id="COG4774">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008287_9_1_5"/>
    <dbReference type="OMA" id="TWGSDQT"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012910">
        <property type="entry name" value="Plug_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037066">
        <property type="entry name" value="Plug_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039423">
        <property type="entry name" value="TonB-dep_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000531">
        <property type="entry name" value="TonB-dep_rcpt_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036942">
        <property type="entry name" value="TonB_rcpt_b-brl_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32552">
        <property type="entry name" value="PTHR32552"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07715">
        <property type="entry name" value="Plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00593">
        <property type="entry name" value="TonB_dep_Rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0798">TonB box</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229266" evidence="6">
        <location>
            <begin position="36"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="domain" description="Plug" evidence="8">
        <location>
            <begin position="80"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="TonB_dep_Rec" evidence="7">
        <location>
            <begin position="282"/>
            <end position="784"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA03966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="785" mass="84643" checksum="E22F23D39AEE1CAA" modified="2005-10-11" version="1">MTKKLEVQLYSSISHLALSAGLAVSLAAIAQPASAQSSQTSSAIALDTINVQGEGGAPSSGEGAYNVTMASSPRQTAPLLDTPQTVTVVPQAVIRQTGARNLTEVLRTTPGISFEGGENGFGTSSNTFQMRGFDASGSLFIDNVRSNGSYTRDIFNVDRVEVFKGAAADNGRGNAGGYINMVTKLPSLQNFTQFDTFSGFSEYGNQPQKRGTVDLNYIVAPNTAVRFNALLEQSGIPGRDLAENKAWGVAPSVALGLGTDFRTFLAFEHLSRRDRPDWGVPGATVPGLVTYNPATMGVRRNAFYGLRSDFDNVDADRVLGRFEYDITRRITISNQTVWAQVDRTTRFTQPTGFTAPATAATSTTFYGRRNQTVSNITNMTIDVDTGPFNHKIATGLEFNHETSDANRFGSISSTTNPALLANLFDPNPDRAGPAPFNPTENNRASVSTAAAYFYDTIKLSEQWQITGGLRAEHYKVDINSRTVAGAPTGLGNFNDTRTTLNGKVGLVYKPVRNASIYGSFGVSHLPPGSYLSNEDISRTGDNAFPGLVQGADPVRFHNYELGSKVDFFNKRLMVAAALFRTEKVNAPITGIDPSIPGDVVGLKGYGKQIVQGVELSVTGALTENWNVFGGAAFMDSRREHSAYLDLVRRTANPGDFTANAGNGPWTSTNGDALAFTPRATANLWTTYHVPATNLTIGGGIRYVGTSYLGRPDTASRIIPNGRFGQLPAYTVVDLMASYELHKDVIVRFNIDNVADNRYAMSTNWSGVRALLGPSRNYRVSTSFRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUT6</accession>
    <name>Q3SUT6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PqqA peptide cyclase</fullName>
            <ecNumber evidence="7">1.21.98.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Coenzyme PQQ synthesis protein E</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pqqE</name>
        <name evidence="9" type="ordered locus">Nwi_0689</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the cross-linking of a glutamate residue and a tyrosine residue in the PqqA protein as part of the biosynthesis of pyrroloquinoline quinone (PQQ).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>[PQQ precursor protein] + S-adenosyl-L-methionine = 5'-deoxyadenosine + E-Y cross-linked-[PQQ precursor protein] + H(+) + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:56836"/>
            <dbReference type="Rhea" id="RHEA-COMP:14800"/>
            <dbReference type="Rhea" id="RHEA-COMP:14801"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:141026"/>
            <dbReference type="ChEBI" id="CHEBI:141027"/>
            <dbReference type="EC" id="1.21.98.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="7">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cofactor biosynthesis; pyrroloquinoline quinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with PqqD. The interaction is necessary for activity of PqqE.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the radical SAM superfamily. PqqE family.</text>
    </comment>
    <dbReference type="EC" id="1.21.98.4" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0689"/>
    <dbReference type="EnsemblBacteria" id="ABA03955">
        <property type="protein sequence ID" value="ABA03955"/>
        <property type="gene ID" value="Nwi_0689"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0689"/>
    <dbReference type="eggNOG" id="COG0535">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009273_4_7_5"/>
    <dbReference type="OMA" id="GWGRQFF"/>
    <dbReference type="UniPathway" id="UPA00539"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009975">
        <property type="term" value="F:cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018189">
        <property type="term" value="P:pyrroloquinoline quinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00660">
        <property type="entry name" value="PqqE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023885">
        <property type="entry name" value="4Fe4S-binding_SPASM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011843">
        <property type="entry name" value="PQQ_synth_PqqE_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017200">
        <property type="entry name" value="PqqE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13186">
        <property type="entry name" value="SPASM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037420">
        <property type="entry name" value="PQQ_syn_pqqE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00280">
        <property type="entry name" value="coenzyme_PQQ_synthesis_protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02109">
        <property type="entry name" value="PQQ_syn_pqqE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04085">
        <property type="entry name" value="rSAM_more_4Fe4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5 7" id="KW-0408">Iron</keyword>
    <keyword evidence="6 7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 7" id="KW-0884">PQQ biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="8">
        <location>
            <begin position="13"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA03955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="389" mass="42098" checksum="49B2BD4D508E87ED" modified="2005-10-11" version="1">MTVTAEGTGQVAAPPPIAMLAELTHRCPLACPYCSNPLELARANEELSTSEWIETFRQAAALGVLHLHLSGGEPASRRDLIDLTKAAVSLGLYTNLITSGLGLTEARVDKLSAAGLDHVQLSIQGTRAPGADHVAGYDGGHARKMAVAGWLRNAGIPLTLNAVCHRRNMGEIEDIISLAVELGARRVEIATVQFHGWAMLNQKALMPTMDQVAKATSIVETMRARLEGALVIDYIPADYYATYPKACMGGWGRAGFNVTPSGRVLPCHAAETIKHLAFDNVRARPLGEIWYNGGAFNAFRGDGWMPEPCRSCERKDIDFGGCRCQAMALAGDASATDPVCRKSPHRKAVDRRIIDNTEVNDTEVRDIEVSDIETPLPPFRYRGNGTLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="126" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUU1</accession>
    <name>Q3SUU1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter, ATPase subunit</fullName>
            <ecNumber evidence="7">3.6.3.25</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0684</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in beta-(1--&gt;2)glucan export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.25" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0684"/>
    <dbReference type="EnsemblBacteria" id="ABA03950">
        <property type="protein sequence ID" value="ABA03950"/>
        <property type="gene ID" value="Nwi_0684"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0684"/>
    <dbReference type="eggNOG" id="COG1116">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_5"/>
    <dbReference type="OMA" id="WPRILSG"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="19"/>
            <end position="26"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="151" mass="16242" checksum="DD360A4A399350B0" modified="2005-10-11" version="1">MLRGIDLHIPAGQFVAIVGRSGCGKSTLLRLIAGLETATAGSIGFGENIRAEDVRVMFQEPRLLPWTKVAANVEVGLGRERARHDARARAEQALTEVGLHDKRGQWPRILSGGQKQRVALARALVSRPRVLALDEPLGALDALTRIDAAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV05</accession>
    <name>Q3SV05_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ErfK/YbiS/YcfS/YnhG</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_0619</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YkuD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313946.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0619"/>
    <dbReference type="MEROPS" id="C82.003"/>
    <dbReference type="EnsemblBacteria" id="ABA03886">
        <property type="protein sequence ID" value="ABA03886"/>
        <property type="gene ID" value="Nwi_0619"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0619"/>
    <dbReference type="eggNOG" id="COG1376">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042399_0_3_5"/>
    <dbReference type="OMA" id="WSGIAKV"/>
    <dbReference type="OrthoDB" id="975147at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071972">
        <property type="term" value="F:peptidoglycan L,D-transpeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16913">
        <property type="entry name" value="YkuD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005490">
        <property type="entry name" value="LD_TPept_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038063">
        <property type="entry name" value="Transpep_catalytic_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03734">
        <property type="entry name" value="YkuD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141523">
        <property type="entry name" value="SSF141523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229239" evidence="4">
        <location>
            <begin position="24"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="YkuD" evidence="5">
        <location>
            <begin position="72"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA03886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="221" mass="24235" checksum="A99A9F5974131CDF" modified="2005-10-11" version="1">MSFLRIKQLGILVAGLMLSGCMATQYQSADTSSFKPRDKDLLAKASYARTPVAASFRRAIVDYHRKEAPGSILVDSDNHYLYYVLDNGKAIRYGVTVGEEALAFSGIARVGNKREWPDWIPTADIHKRMGGLPSRVAGGPDNPLGARALYLYQGSKDTLFRIHGTNQPEYIGTSISSGCIRMTNEDAIDLYDRVKVGTMVVVLEPRRSARMASWGGNGSVN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-09-02" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV21</accession>
    <name>MNMA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA-specific 2-thiouridylase MnmA</fullName>
            <ecNumber evidence="1">2.8.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mnmA</name>
        <name type="ordered locus">Nwi_0603</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AH2 + ATP + S-sulfanyl-L-cysteinyl-[protein] + uridine(34) in tRNA = 2-thiouridine(34) in tRNA + A + AMP + diphosphate + H(+) + L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:47032"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11726"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="Rhea" id="RHEA-COMP:11728"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:87170"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.8.1.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MnmA/TRMU family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.13" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313930.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SV21"/>
    <dbReference type="SMR" id="Q3SV21"/>
    <dbReference type="STRING" id="323098.Nwi_0603"/>
    <dbReference type="EnsemblBacteria" id="ABA03870">
        <property type="protein sequence ID" value="ABA03870"/>
        <property type="gene ID" value="Nwi_0603"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0603"/>
    <dbReference type="eggNOG" id="COG0482">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035188_0_1_5"/>
    <dbReference type="OMA" id="AVCTGHY"/>
    <dbReference type="OrthoDB" id="1054741at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01998">
        <property type="entry name" value="tRNA_Me_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00144">
        <property type="entry name" value="tRNA_thiouridyl_MnmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023382">
        <property type="entry name" value="Adenine_a_hdrlase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004506">
        <property type="entry name" value="tRNA-specific_2-thiouridylase"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00420">
        <property type="entry name" value="trmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000349716" description="tRNA-specific 2-thiouridylase MnmA">
        <location>
            <begin position="1"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with tRNA" evidence="1">
        <location>
            <begin position="160"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine persulfide intermediate" evidence="1">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="19"/>
            <end position="26"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with tRNA" evidence="1">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with tRNA" evidence="1">
        <location>
            <position position="352"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Alternate" evidence="1">
        <location>
            <begin position="113"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00144"/>
        </source>
    </evidence>
    <sequence length="400" mass="43383" checksum="1E84C4D279A41390" modified="2005-10-11" version="1">MLNSLDLEGRPQDTRVVVAMSGGVDSSVTAALLKSEGYDVVGITLQLYDHGAATHRKGACCAGQDIHDARNVAARLGVPHYVLDYESRFRETVIENFADSYASGETPVPCIECNRQVKFRDLLATARELGAAALATGHYVSSRRLADGSRALLCAADTDRDQSYFLFATTREQLDFLRFPLGDMTKQQTRELARRFGLSVADKHDSQDICFVPTGRYTDIISRLKPNAMVSGDIVDLDGHVIGHHEGIVHFTVGQRRGLGIASGAPLYVLSLDAANRRVVVGPREALKMHRIVLRDVNWIGDGALDRAIGAGLELFVRVRSTRRPQPAWLRVVDGRYEVELVAGEEGVSPGQACVFYDGAEGQSRVLGGGFIARAMAQRANEAAAQSGTLAQSFAAELRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV22</accession>
    <name>Q3SV22_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Flagellar hook-length control protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0602</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0602"/>
    <dbReference type="EnsemblBacteria" id="ABA03869">
        <property type="protein sequence ID" value="ABA03869"/>
        <property type="gene ID" value="Nwi_0602"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0602"/>
    <dbReference type="eggNOG" id="COG3144">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038554_0_0_5"/>
    <dbReference type="OMA" id="MRRNIDQ"/>
    <dbReference type="OrthoDB" id="1989832at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021136">
        <property type="entry name" value="Flagellar_hook_control-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038610">
        <property type="entry name" value="FliK-like_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02120">
        <property type="entry name" value="Flg_hook"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0282">Flagellum</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flg_hook" evidence="2">
        <location>
            <begin position="400"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="259"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="304"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="320"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="528" mass="54104" checksum="9C2CA93D289163CE" modified="2005-10-11" version="1">MSSLFSLPQHGPVLAACLDDSISKRLAVRSVTTEPTASVSLQSAPPSSQRGDRPFGDAFGTLVDASMQGSDVAVVQPGNSRDPLNARPSVPRNFQASSAPPPQSNPVETGRDIRQSSVRAERPSAAAPHEPKHDDPEIAPTGNAFDPPQEVSDTADPGASPETMATPPAVVVPVATPVVVIPADPGPDAAEAPLAQQTGTTETSPANAGIDPTLATDTEAVAAAVLQEMPDTSQALRTVMQTEGALAAAVATAAPGTAKAAGTLDRAPPGGTTASVSTGIQPEPDMPALPAQPAAGGDPVPPHDAALADHRHDAAESAKNAASETATFSTQTATSAPHPARAPDAAAAQPQSGQVEGLDINGALAQPQTTSNATTPATPQYNVAMATGAAVPLNGLAVQIAVTALSGRSRFEIRLDPADLGRIDVRLDVDRQGRVTSHLTVEKPETLAMLRQDAPHLQRALQDAGLRTSDGGLQFSLRDQSSQEHDARDGAGRHNRHLVVTEEDIPVAAAGRDYGRILGSNRGLDIRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV26</accession>
    <name>Q3SV26_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Flagellar motor switch protein FliG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Nwi_0598</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">FliG is one of three proteins (FliG, FliN, FliM) that forms the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the motor that determine the direction of flagellar rotation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Bacterial flagellum basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cell inner membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
            <orientation evidence="10">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the FliG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313926.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0598"/>
    <dbReference type="EnsemblBacteria" id="ABA03865">
        <property type="protein sequence ID" value="ABA03865"/>
        <property type="gene ID" value="Nwi_0598"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0598"/>
    <dbReference type="eggNOG" id="COG1536">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047835_0_0_5"/>
    <dbReference type="OMA" id="QVRPFEF"/>
    <dbReference type="OrthoDB" id="847822at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000090">
        <property type="entry name" value="Flg_Motor_Flig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023087">
        <property type="entry name" value="Flg_Motor_Flig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011002">
        <property type="entry name" value="FliG_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032779">
        <property type="entry name" value="FliG_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028263">
        <property type="entry name" value="FliG_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30534">
        <property type="entry name" value="PTHR30534"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01706">
        <property type="entry name" value="FliG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14841">
        <property type="entry name" value="FliG_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14842">
        <property type="entry name" value="FliG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003161">
        <property type="entry name" value="FliG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00954">
        <property type="entry name" value="FLGMOTORFLIG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48029">
        <property type="entry name" value="SSF48029"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00207">
        <property type="entry name" value="fliG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="10" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="5 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6 10" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="14" id="KW-0969">Cilium</keyword>
    <keyword evidence="7 10" id="KW-0283">Flagellar rotation</keyword>
    <keyword evidence="14" id="KW-0282">Flagellum</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FliG_N" evidence="13">
        <location>
            <begin position="35"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="FliG_M" evidence="12">
        <location>
            <begin position="145"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="FliG_C" evidence="11">
        <location>
            <begin position="246"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR003161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF14841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF14842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABA03865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="363" mass="40489" checksum="4FEEA94214F35920" modified="2005-10-11" version="1">MTVPQTTTNQNANDINSVVASLASRQGARPRSKPMSGPQRAAVMMLALGEQYGGKVWAMLDDDEVRDLSLAMSTLGTVEADVVEDLLLEFVSRMSASGALMGNFDATERLLNQYLPAERVSGIMDEIRGPAGRNMWEKLSNVQEEVLANYLKNEYPQTIAVVLSKLKPEHAARVLAILPEDMALDVVGRMLRMEAVQKEVIERVEQTLRSEFMSNLSQTRRRDAHEVMAEIFNNFDRQTETRFITALEDENREAAERIKALMFTFDDLVKLDAGSAQTLMRHVDKDKLGIALKSANEEVRAFFMGNMSTRAAKMLMDDMAALGPVRLRDVDEAQALLVNLAKDLAAKGEIMLSKNRADDELVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV47</accession>
    <name>Q3SV47_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0577</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0577"/>
    <dbReference type="EnsemblBacteria" id="ABA03844">
        <property type="protein sequence ID" value="ABA03844"/>
        <property type="gene ID" value="Nwi_0577"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0577"/>
    <dbReference type="eggNOG" id="COG2177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067538_0_0_5"/>
    <dbReference type="OMA" id="RMRSMTG"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004513">
        <property type="entry name" value="ABC_transpt_FtsX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47755">
        <property type="entry name" value="PTHR47755"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="282"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="314" mass="33620" checksum="EBACCCCED60761B3" modified="2005-10-11" version="1">MLMDLGRERPQVPVRARNMSPIVPRASIAGRALVAVVAIMTFLASITTGAVLLINASASEWQSEVASEITLQVRPAAGRDLEQDVAAAAAATREQPGILDVRPYTREESAKLLEPWLGSGLSLDDLPVPRMIVARAAPGTRLDLEGLRSRVTQLAPSTSVDDHRAWIDRMRSMTNATVFAGIGVLALVIIATIISVSFATRGAMAANRPIVEVLHFVGASDRYIANHFLRHFLRLGLRGGVIGCVAAMLMFGFSESIANWFSGTPVGDQFAALLGTFSLRPSGYLVLAGQTILIAAITAFASRRTLFATLDEID</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVE3</accession>
    <name>Q3SVE3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Integrase, catalytic region</fullName>
            <ecNumber evidence="3">2.7.7.49</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0308</name>
        <name evidence="3" type="ordered locus">Nwi_0481</name>
        <name evidence="4" type="ordered locus">Nwi_1097</name>
        <name evidence="5" type="ordered locus">Nwi_1296</name>
        <name evidence="6" type="ordered locus">Nwi_1717</name>
        <name evidence="7" type="ordered locus">Nwi_2818</name>
    </gene>
    <organism evidence="3 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="3 8" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="3">Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313641.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0308"/>
    <dbReference type="EnsemblBacteria" id="ABA03575">
        <property type="protein sequence ID" value="ABA03575"/>
        <property type="gene ID" value="Nwi_0308"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03748">
        <property type="protein sequence ID" value="ABA03748"/>
        <property type="gene ID" value="Nwi_0481"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04359">
        <property type="protein sequence ID" value="ABA04359"/>
        <property type="gene ID" value="Nwi_1097"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04557">
        <property type="protein sequence ID" value="ABA04557"/>
        <property type="gene ID" value="Nwi_1296"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04978">
        <property type="protein sequence ID" value="ABA04978"/>
        <property type="gene ID" value="Nwi_1717"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06071">
        <property type="protein sequence ID" value="ABA06071"/>
        <property type="gene ID" value="Nwi_2818"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0308"/>
    <dbReference type="KEGG" id="nwi:Nwi_0481"/>
    <dbReference type="KEGG" id="nwi:Nwi_1097"/>
    <dbReference type="KEGG" id="nwi:Nwi_1296"/>
    <dbReference type="KEGG" id="nwi:Nwi_1717"/>
    <dbReference type="KEGG" id="nwi:Nwi_2818"/>
    <dbReference type="HOGENOM" id="CLU_027402_15_1_5"/>
    <dbReference type="OMA" id="DRACEEN"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13683">
        <property type="entry name" value="rve_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="127"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA04978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA06071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="316" mass="36557" checksum="58620D96407A8C67" modified="2005-10-11" version="1">MGQVLHGSATTTEAIRRAIQNSEESLRALSKRYGINQKTVAKWKKRTSVADLPTGPKDPRSTVLSSEEEAIIVAFRKHTLLPLDDCLYALQPTIPHLSRSSLHRCLHRHGISRLPEVEGDKPIRKTFRSYPIGYFHIDIAEVQTAEGKLRLFVAIDRTSKFAYAELHQEAGKMVAAQFLRNLIVAVPYAIHTVLTDNGIQFTNHARHKYAFHHIFDRVCDENGIEHRLTRINHPWTNGQVERMNRTIKDATVKRFHYDNHDQLRRHLQDFIKAYNFGRRLKTLKGLTPYEFICKRWTSEPDRFIIDPIHQMPGLNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVI7</accession>
    <name>Q3SVI7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0437</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313768.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03704">
        <property type="protein sequence ID" value="ABA03704"/>
        <property type="gene ID" value="Nwi_0437"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0437"/>
    <dbReference type="eggNOG" id="COG1672">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_458443_0_0_5"/>
    <dbReference type="OrthoDB" id="1492261at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="571"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="595" mass="67525" checksum="5288B9591BE85C5B" modified="2005-10-11" version="1">MLETYLKDRARVVADGLFQPVITGGKHPQNNLTAELRKHGSNPRITGRVQLIVGAVGSGKSLFIRRFFKRLMPEELQKKTLWAFLNFNSELKSPEELRAAVCELFIQSFSELNNIDIEDLDFQEKIFASEMAAFDRGPAKILKTGNPQQYNQQRYFRLKELREDNEKIVSAISRHFSGEKRIGLVVVFDNVDKRSRDVQLAIFEAAQWFKELTRALVIVNLRDTTFEAHRDEPPLDAFINAVNFWSPRFALMIKKRLEIVMENIEQGEQLGKFQRFTLESGAQVLYKSGRLGEFLMSIYASLFDRRASSIGAALESLAGKNARSALGMFADIIASPHVPTSQIGSTAAASEVARIEEDRIVRALMRGRYRLFNNRLAYVRNVLSPVPHAMRPSNFLYSDILEFLIRHRKEKIDFSVEGYASGRTIVNRMGQLGYDEDDAFSGLQQLVKWNLIEPESLLVEDLTLEDPVQVHASGFIHMRYFLKRPEYLFGITADMGFATYALTEEAASVWGSASGQGEPRFRARQRILNNLADYFKAEYDRRIRRHAFYEDLGYGGKGVVFASRLVADQIGKPPPRAAAPATRSPGHRQKGAKNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVI8</accession>
    <name>Q3SVI8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Type I site-specific deoxyribonuclease</fullName>
            <ecNumber evidence="3">3.1.21.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_0436</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates, ATP is simultaneously hydrolyzed.</text>
            <dbReference type="EC" id="3.1.21.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HsdR family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.3" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313767.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0436"/>
    <dbReference type="EnsemblBacteria" id="ABA03703">
        <property type="protein sequence ID" value="ABA03703"/>
        <property type="gene ID" value="Nwi_0436"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0436"/>
    <dbReference type="eggNOG" id="COG0610">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1128148_0_0_5"/>
    <dbReference type="OrthoDB" id="1492261at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009035">
        <property type="term" value="F:type I site-specific deoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007409">
        <property type="entry name" value="Restrct_endonuc_type1_HsdR_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04313">
        <property type="entry name" value="HSDR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0680">Restriction system</keyword>
    <feature type="domain" description="HSDR_N" evidence="11">
        <location>
            <begin position="71"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA03703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="246" mass="28071" checksum="4DAD1B893CE28E02" modified="2005-10-11" version="1">MIAKLKPRDRIQTPVTNLSEFNTESDVEQKLVYPFLVHPSFMDIPSEWVRTKEYMEPTEIDKGAGKRVGYIPDYSVWRSGFPLLVVEAKRPDEPIEKAIREAHLYANRINNRYPPNVNPIRYVLACNGEQFALAPFDSETELLYAKAVDLRPGTDILAAFKAILNKDEFEKRAQEMNVHFQSRRFTRVPSLLTGTQVTEQLGINPFAQELFPTSGKRPTRRRTTSLIAAMSRPRSAASMARCSKPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVR1</accession>
    <name>Q3SVR1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ISRm2011-2 transposase protein</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="3">Probable ISRm2011-2 transposase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0232</name>
        <name evidence="4" type="ordered locus">Nwi_0363</name>
        <name evidence="5" type="ordered locus">Nwi_0483</name>
        <name evidence="6" type="ordered locus">Nwi_0654</name>
        <name evidence="7" type="ordered locus">Nwi_0707</name>
        <name evidence="8" type="ordered locus">Nwi_0851</name>
        <name evidence="9" type="ordered locus">Nwi_1315</name>
        <name evidence="10" type="ordered locus">Nwi_1396</name>
        <name evidence="11" type="ordered locus">Nwi_1968</name>
        <name evidence="12" type="ordered locus">Nwi_2029</name>
        <name evidence="13" type="ordered locus">Nwi_2060</name>
        <name evidence="14" type="ordered locus">Nwi_2104</name>
        <name evidence="15" type="ordered locus">Nwi_2847</name>
    </gene>
    <organism evidence="4 16">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="4 16" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="4">Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0232"/>
    <dbReference type="EnsemblBacteria" id="ABA03500">
        <property type="protein sequence ID" value="ABA03500"/>
        <property type="gene ID" value="Nwi_0232"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03630">
        <property type="protein sequence ID" value="ABA03630"/>
        <property type="gene ID" value="Nwi_0363"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03750">
        <property type="protein sequence ID" value="ABA03750"/>
        <property type="gene ID" value="Nwi_0483"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03920">
        <property type="protein sequence ID" value="ABA03920"/>
        <property type="gene ID" value="Nwi_0654"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03973">
        <property type="protein sequence ID" value="ABA03973"/>
        <property type="gene ID" value="Nwi_0707"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04115">
        <property type="protein sequence ID" value="ABA04115"/>
        <property type="gene ID" value="Nwi_0851"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04576">
        <property type="protein sequence ID" value="ABA04576"/>
        <property type="gene ID" value="Nwi_1315"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04657">
        <property type="protein sequence ID" value="ABA04657"/>
        <property type="gene ID" value="Nwi_1396"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05228">
        <property type="protein sequence ID" value="ABA05228"/>
        <property type="gene ID" value="Nwi_1968"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05288">
        <property type="protein sequence ID" value="ABA05288"/>
        <property type="gene ID" value="Nwi_2029"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05318">
        <property type="protein sequence ID" value="ABA05318"/>
        <property type="gene ID" value="Nwi_2060"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05359">
        <property type="protein sequence ID" value="ABA05359"/>
        <property type="gene ID" value="Nwi_2104"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06097">
        <property type="protein sequence ID" value="ABA06097"/>
        <property type="gene ID" value="Nwi_2847"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0232"/>
    <dbReference type="KEGG" id="nwi:Nwi_0363"/>
    <dbReference type="KEGG" id="nwi:Nwi_0483"/>
    <dbReference type="KEGG" id="nwi:Nwi_0654"/>
    <dbReference type="KEGG" id="nwi:Nwi_0707"/>
    <dbReference type="KEGG" id="nwi:Nwi_0851"/>
    <dbReference type="KEGG" id="nwi:Nwi_1315"/>
    <dbReference type="KEGG" id="nwi:Nwi_1396"/>
    <dbReference type="KEGG" id="nwi:Nwi_1968"/>
    <dbReference type="KEGG" id="nwi:Nwi_2029"/>
    <dbReference type="KEGG" id="nwi:Nwi_2060"/>
    <dbReference type="KEGG" id="nwi:Nwi_2104"/>
    <dbReference type="KEGG" id="nwi:Nwi_2847"/>
    <dbReference type="eggNOG" id="COG3335">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056788_10_0_5"/>
    <dbReference type="OMA" id="WISETSI"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0814">Transposable element</keyword>
    <feature type="domain" description="DDE_3" evidence="2">
        <location>
            <begin position="44"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA03920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA04115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA04576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA04657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA05228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA05288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA05318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABA05359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABA06097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="213" mass="24544" checksum="50FEF3644D47F34D" modified="2005-10-11" version="1">MGVRSRCEAQLQKKTLIAAEQDRPDVARRRAQWTKYRDRIDPTRLVFIDETWTKTNMAPLRGWAPRGQRIRAKVPHGRWQTMTFMAALRHDRITAPWFIEGPINGEAFLLYIEKVLVPTLRHGDIVIMDNLGSHKASAVRRVIRAAGARLFYLPKYSPDLNPIEQFFAKFKHWLRKAAQRTTEAVYNAIAPILETVAPAECANYFVNAGYNQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVU6</accession>
    <name>Q3SVU6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Amylo-alpha-1,6-glucosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0328</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0328"/>
    <dbReference type="EnsemblBacteria" id="ABA03595">
        <property type="protein sequence ID" value="ABA03595"/>
        <property type="gene ID" value="Nwi_0328"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0328"/>
    <dbReference type="eggNOG" id="COG3408">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019216_1_0_5"/>
    <dbReference type="OMA" id="WASATPF"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032790">
        <property type="entry name" value="GDE_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032856">
        <property type="entry name" value="GDE_N_bis"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06202">
        <property type="entry name" value="GDE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14742">
        <property type="entry name" value="GDE_N_bis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GDE_N_bis" evidence="2">
        <location>
            <begin position="38"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="GDE_C" evidence="1">
        <location>
            <begin position="291"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="733" mass="81835" checksum="76AB478B7AC5CCA5" modified="2005-10-11" version="1">MMATKRAARMTSAPSEALSESPFYIPMTGPSARPRRTLKHDDTFVVLDSHGDIGASAGGPDGLFNSDTRYLARLELHLNDMQPLLLGSNLRNDNSGLTVDLTNPDIYDGDRIVFHKDTIHIVRTTFLWRGTAYQRIGLQNHGEQTASIALSLRFDNDFADLFEVRGERRERRGTGASELADPATVVLSYTGVDDIVRTSRLHFAPRPTRLTVNSAVYQIDLPPQQTTEIFLTVSCNKPETERPVAFYRGLLAHHREMRRAWSHTTSIETSNDVFNEVLCQSMADLNILMTQTPQGRYPYAGIPWYSTTFGRDGLITALQMLWIDPGIARGVLRRLAAFQAKTVDPLADAEPGKILHEMRGGEMAALREVPFERYYGSVDSTPLFVLLAGLYVERTGDESILKELWPAIEAALGWIDGPGDPDGDGFVEYRRASEQGLANQGWKDSFDAIFHADGTLAEGNIALAEVQGYVFAAKRAAARCARALGLTDRAGELDRAAERLARRFEETFWLPDLGTYALALDGEKRPCQVRTSNAGQLLFTGMVREDRARLVAADLMRPAFFTGWGIRTVALGEARYNPMSYHNGSIWPHDNALIALGLGRYGLKHSVEQVFKGLFDAASYMDLRRLPELFCGFRREPRRGPTLYPVACLPQAWASATPFTLLEAALGLEFDPARSEIRLRNPRLPAFLNEMILRRLHLGESTVDLRLRRHDEDVSVEVLRTQGHVQVSMVLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVU8</accession>
    <name>Q3SVU8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase, group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0326</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313658.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0326"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03593">
        <property type="protein sequence ID" value="ABA03593"/>
        <property type="gene ID" value="Nwi_0326"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0326"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042257_1_0_5"/>
    <dbReference type="OMA" id="FHLDYYP"/>
    <dbReference type="OrthoDB" id="694191at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="2">
        <location>
            <begin position="18"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="170"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="355" mass="39705" checksum="FC1C079313A81395" modified="2005-10-11" version="1">MRIAQVAPLTEAVPPKLYGGTERVVHWLTEELVALGHDVTLFASGDSLTSARLDATWPRALRLDGAVRDPNALHMVMLERVRRKCDDEEFDFLHFHLDYYPFSLFSRQPTPFLTTLHGRLDLPELQPVFNAFSSIPLISISNAQRRPAPQARWLRTVYHGLPEKLLTPRPVKPSYLAVLGRIAPEKGVDRAIRIALRCGIPLKIAAKVDRADQDYYDECIRPMMDHPLVEYIGEISDHEKPDFLSGAIGLLVPIAWPEPFGLVMIEAMACGTPVIAYNRGSVAEIITDGRTGFIVEDEISAVAAVHSLTTLDRGAIRAEFEARFTARRMALDYLDSYRNLIEATAPRIKLVSNAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW98</accession>
    <name>Q3SW98_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0175</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03443">
        <property type="protein sequence ID" value="ABA03443"/>
        <property type="gene ID" value="Nwi_0175"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0175"/>
    <dbReference type="HOGENOM" id="CLU_150627_0_0_5"/>
    <dbReference type="OMA" id="YCYRTHA"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229307" evidence="2">
        <location>
            <begin position="23"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="95" mass="10553" checksum="0A2B1C27C173E682" modified="2005-10-11" version="1">MLKTSLIAVAAAGFLLALPASAADFSFTDEAPQMICDHDGNCYSAHPPRFAERWQDDDNGDDVYERRDYHSRYAPPSEFHRDDDGPATGYDGGPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWN8</accession>
    <name>Q3SWN8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ferric uptake regulator, Fur family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as a global negative controlling element, employing Fe(2+) as a cofactor to bind the operator of the repressed genes.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313374.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03303">
        <property type="protein sequence ID" value="ABA03303"/>
        <property type="gene ID" value="Nwi_0035"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0035"/>
    <dbReference type="eggNOG" id="COG0735">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096072_1_1_5"/>
    <dbReference type="OMA" id="LNGCPWH"/>
    <dbReference type="OrthoDB" id="1630127at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07153">
        <property type="entry name" value="Fur_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002481">
        <property type="entry name" value="FUR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33202">
        <property type="entry name" value="PTHR33202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01475">
        <property type="entry name" value="FUR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="168" mass="18925" checksum="5D81EF383C151F53" modified="2005-10-11" version="1">MPGVSTSENIVVVTEEDRDIAAIGHGRQPTLTGCPWHDVNEMLQSVGLRPTRQRMALGWLLFGKGARHLTAEMLYEEATLAKVPVSLATVYNTLNQLTDAGLLRQVSVDGTKTYFDTNVTAHHHFYLENNHELVDIPDPHLVLQKMPNVPEGYEISRIDMVVRLRKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMU0</accession>
    <name>Q3SMU0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Peptidase M22, glycoprotease</fullName>
            <ecNumber evidence="3">3.4.24.57</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0011</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.4.24.57" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313350.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0011"/>
    <dbReference type="EnsemblBacteria" id="ABA03279">
        <property type="protein sequence ID" value="ABA03279"/>
        <property type="gene ID" value="Nwi_0011"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0011"/>
    <dbReference type="eggNOG" id="COG1214">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064886_3_0_5"/>
    <dbReference type="OMA" id="TASIYCA"/>
    <dbReference type="OrthoDB" id="1826274at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022496">
        <property type="entry name" value="T6A_TsaB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="PTHR11735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF11">
        <property type="entry name" value="PTHR11735:SF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03725">
        <property type="entry name" value="T6A_YeaZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TsaD" evidence="2">
        <location>
            <begin position="34"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="232" mass="23933" checksum="26C9835AAE7F7B3C" modified="2005-10-11" version="1">MLVLAIDTALDACAAGILDTDEGRLIAQETLPMKRGHAETLMPLIARVIESAGIGFKALDRIAATTGPGSFTGLRVGLSAARGIALAADKPVVGVTTLAAFAAPVIGEDRKPPVISAIDARHDQVYFQAMSGDGAALIQPRVAPVAEALAASRFGAPHLVGNAARLLADRWPAHAPPPVAVDQQAAPDIVWVAWLGAAVSPDAAPPRPYYLRAPDARPTGDTLLKPRTLAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMY4</accession>
    <name>Q3SMY4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_3110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316268.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3110"/>
    <dbReference type="EnsemblBacteria" id="ABA06357">
        <property type="protein sequence ID" value="ABA06357"/>
        <property type="gene ID" value="Nwi_3110"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3110"/>
    <dbReference type="eggNOG" id="COG4680">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153067_3_1_5"/>
    <dbReference type="OMA" id="IKFVGTH"/>
    <dbReference type="OrthoDB" id="2072230at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110001">
        <property type="term" value="C:toxin-antitoxin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018669">
        <property type="entry name" value="Toxin_HigB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09907">
        <property type="entry name" value="HigB_toxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="103" mass="11714" checksum="69CCF8EE9C9119D4" modified="2005-10-11" version="1">MLVIGTDIVEQYFAERSGHRGIDAARSQYRAWLAIAGASDWRTPQDVKRSHPKASILKGGRVVFNIKANDFRLIALMQYRDGVVMIRFFGSHEEYDKVNAETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN45</accession>
    <name>Q3SN45_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_3047</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316212.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3047"/>
    <dbReference type="EnsemblBacteria" id="ABA06296">
        <property type="protein sequence ID" value="ABA06296"/>
        <property type="gene ID" value="Nwi_3047"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3047"/>
    <dbReference type="eggNOG" id="ENOG50333E7">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_185250_0_0_5"/>
    <dbReference type="OMA" id="ITVISHY"/>
    <dbReference type="OrthoDB" id="1976038at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="106" mass="11942" checksum="6C06D7FCDEB6FCAC" modified="2005-10-11" version="1">MIEVQRIDDGNPLGFEVVVREGKGETRHRVTMSEAGSRQLTAGKHTPERCIEAAFRFLLDREPKESILARFDISVISRYFPEFEQELPGYLSAGQEDSRSGKRGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNA6</accession>
    <name>Q3SNA6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Isocitrate lyase</fullName>
            <ecNumber evidence="1">4.1.3.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Isocitrase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Isocitratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_2985</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>D-threo-isocitrate = glyoxylate + succinate</text>
            <dbReference type="Rhea" id="RHEA:13245"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="EC" id="4.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Can also use Mn(2+) ion.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.1" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316157.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2985"/>
    <dbReference type="EnsemblBacteria" id="ABA06235">
        <property type="protein sequence ID" value="ABA06235"/>
        <property type="gene ID" value="Nwi_2985"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2985"/>
    <dbReference type="eggNOG" id="COG2224">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019214_2_0_5"/>
    <dbReference type="OMA" id="LEKDWAE"/>
    <dbReference type="OrthoDB" id="152545at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004451">
        <property type="term" value="F:isocitrate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00377">
        <property type="entry name" value="ICL_PEPM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039556">
        <property type="entry name" value="ICL/PEPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006254">
        <property type="entry name" value="Isocitrate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018523">
        <property type="entry name" value="Isocitrate_lyase_ph_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21631">
        <property type="entry name" value="PTHR21631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00463">
        <property type="entry name" value="ICL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001362">
        <property type="entry name" value="Isocit_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01346">
        <property type="entry name" value="isocit_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00161">
        <property type="entry name" value="ISOCITRATE_LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="84"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="184"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="307"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001362-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001362-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001362-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA06235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="435" mass="47596" checksum="1F0D30007A91F8F7" modified="2005-10-11" version="1">MSRTFEQIVLAPKGRFEGISRPYAPEDVERLRGSVQIEHTLAERGANRLWKQLNEMPYVNSLGAVTGNQAMQQARAGLPAIYLSGWQVAADANTAGAMYPDQSLYPANAGPELCRRINRTFQRADQIEHAEGGAKIDWFVPIVADAEAGFGGPLNAFEIMKAYIEAGAAGVHFEDQLASEKKCGHLGGKVLIPTAEHERNLIAARLAADVCGVPTLVLARTDAESAKLITSDIDERDHEFITGERTAEGFYRLKPGTGLDHCIKRGLSFAKYADLLWWETSKPDLEGARAFAEAVRKVYPDKMLAYNCSPSFNWEANLDKAAIAHFQKEIAAMGYKFQFVTLAGFHSLNHGMFELARGYKAEGMAAYSRLQQAEFASEKFGYSATRHQREVGTGYFDLVSATITGGQSSTTALHDSTEAAQFKSQGSPINVQAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNB0</accession>
    <name>Q3SNB0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glutathione S-transferase</fullName>
            <ecNumber evidence="4">2.5.1.18</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2981</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316153.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2981"/>
    <dbReference type="EnsemblBacteria" id="ABA06231">
        <property type="protein sequence ID" value="ABA06231"/>
        <property type="gene ID" value="Nwi_2981"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2981"/>
    <dbReference type="eggNOG" id="COG0625">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011226_6_0_5"/>
    <dbReference type="OMA" id="TCLWESN"/>
    <dbReference type="OrthoDB" id="1752966at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="SSF47616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="84"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA06231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="216" mass="24184" checksum="979EB6C1E874688C" modified="2005-10-11" version="1">MYKLYSMQRSGNSYKVRLALTVLDVPYHAIEIDILRGESRTPDFLAKNPSGQVPLLEVAEGRYLAESNAILWYVAIGTRLAPDSPIERAEALQWMFFEQHALEPNIGAAYFWLSLVKGGRDLQTHALDDWMERGYAALQVMENHLRTHDFFAAGQLTIADIALYAYTHLADQCDFDLTTFPSVRAWLRRIEQQPGFITMDGSAASSGNAAVGIARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNC9</accession>
    <name>Q3SNC9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2962</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06212">
        <property type="protein sequence ID" value="ABA06212"/>
        <property type="gene ID" value="Nwi_2962"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2962"/>
    <dbReference type="eggNOG" id="ENOG5032RYR">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116315_2_0_5"/>
    <dbReference type="OMA" id="WHITARM"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025187">
        <property type="entry name" value="DUF4112"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35519">
        <property type="entry name" value="PTHR35519"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13430">
        <property type="entry name" value="DUF4112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="180" mass="19690" checksum="89AE04D853F7828E" modified="2005-10-11" version="1">MKSMPNDDLFSRDNAGARRSARAKIIDQDGREVHGDPPGADGFQTGPFGAFQHGASGFEFAAAFQPLTREQRLARLDAIAKLLDVAFVVPGTKVRYGIDGLIGLVPVIGDLITTAFSLWLVREARALGAPWHITARMLSNVAVDGVVGLVPVAGDAFDVMFRANMRNVRMFKRWLDRQPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNG9</accession>
    <name>Q3SNG9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ISBm1, transposase orfA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2920</name>
        <name evidence="2" type="ordered locus">Nwi_2922</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="2">Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06170">
        <property type="protein sequence ID" value="ABA06170"/>
        <property type="gene ID" value="Nwi_2920"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06172">
        <property type="protein sequence ID" value="ABA06172"/>
        <property type="gene ID" value="Nwi_2922"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2920"/>
    <dbReference type="KEGG" id="nwi:Nwi_2922"/>
    <dbReference type="eggNOG" id="COG3293">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055261_2_2_5"/>
    <dbReference type="OMA" id="YSRFNGW"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025161">
        <property type="entry name" value="Put_IS4/IS5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13340">
        <property type="entry name" value="DUF4096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="121" mass="13769" checksum="B7817B04224B6447" modified="2005-10-11" version="1">MARFDLTDFEWSVIQPLLPNKSRGVARVDDRRVLNGIFWRLRTGAPWADIPARYGPHTTCVNRFNRWRKAGVWDRILSAVSKAYDGGIQMIDSSSIRVHQHAANGQKNRRDPVAWVARAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNQ0</accession>
    <name>Q3SNQ0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Integrase, catalytic region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2839</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2839"/>
    <dbReference type="EnsemblBacteria" id="ABA06091">
        <property type="protein sequence ID" value="ABA06091"/>
        <property type="gene ID" value="Nwi_2839"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2839"/>
    <dbReference type="eggNOG" id="COG2801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027402_26_2_5"/>
    <dbReference type="OMA" id="MIRTVPY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13683">
        <property type="entry name" value="rve_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="153" mass="18168" checksum="DE5B991BEC045882" modified="2005-10-11" version="1">MVAPCDLQLHVAAQFLRNLIVAVPYAIHTVLTDNGIQFTNHARHKYAFHHIFDRVCDENGIEHRLTRINHPWTNGQVERMNRTIKDATVKRFHYDNHDQLRRHLQDFIKAYNFGRRLKTLKGLTPYEFICKRWTSEPDRFIIDPIHQMPGLNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNT7</accession>
    <name>Q3SNT7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Succinate dehydrogenase iron-sulfur subunit</fullName>
            <ecNumber evidence="10">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Nwi_2801</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a quinone + succinate = a quinol + fumarate</text>
            <dbReference type="Rhea" id="RHEA:40523"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="10">Binds 1 [2Fe-2S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
        <text evidence="10">Binds 1 [3Fe-4S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="10">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (bacterial route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur, cytochrome b-556, and a hydrophobic anchor protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="10"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315999.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2801"/>
    <dbReference type="EnsemblBacteria" id="ABA06054">
        <property type="protein sequence ID" value="ABA06054"/>
        <property type="gene ID" value="Nwi_2801"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2801"/>
    <dbReference type="eggNOG" id="COG0479">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044838_0_2_5"/>
    <dbReference type="OMA" id="DGQYFGP"/>
    <dbReference type="OrthoDB" id="1605514at2"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER01005"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004489">
        <property type="entry name" value="Succ_DH/fum_Rdtase_Fe-S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025192">
        <property type="entry name" value="Succ_DH/fum_Rdtase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13085">
        <property type="entry name" value="Fer2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00384">
        <property type="entry name" value="dhsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="9 10" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 10" id="KW-0408">Iron</keyword>
    <keyword evidence="8 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="12">
        <location>
            <begin position="30"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="13">
        <location>
            <begin position="161"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABA06054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="260" mass="29608" checksum="7688DB0673AA50B7" modified="2005-10-11" version="1">MVKFALPRKSKVTEGKTWPKPGSATETREFKVYRWNPDDDENPRVDTFHVDLHECGPRVLDGLIWIKDHIDPTLTFRRSCREGVCGSCAMNINGQNTLACTQSLHDIPGDAVKVNPLPHQPVVKDLVPDLTNFYAQYASIEPWLKTATPTPQKEWRQSREDREKLDGLYECILCACCSTACPSYWWNSDRFLGPAALLQAARWVTDSRDEATGERLDYLEDPFRLYRCHTILNCTKACPKSLNPAEAIATLKLKLVERQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPE1</accession>
    <name>Q3SPE1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PMT_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_2597</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315800.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2597"/>
    <dbReference type="EnsemblBacteria" id="ABA05850">
        <property type="protein sequence ID" value="ABA05850"/>
        <property type="gene ID" value="Nwi_2597"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2597"/>
    <dbReference type="eggNOG" id="COG1807">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028670_0_0_5"/>
    <dbReference type="OMA" id="REYQVGT"/>
    <dbReference type="OrthoDB" id="854536at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038731">
        <property type="entry name" value="RgtA/B/C-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13231">
        <property type="entry name" value="PMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="167"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="242"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="290"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="315"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="350"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="PMT_2" evidence="10">
        <location>
            <begin position="63"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="435"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="440"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA05850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="522" mass="55987" checksum="59092D6425D82214" modified="2005-10-11" version="1">MRFTSLVVELIRARPLLVFWIVALFQAAMWLLVPLLLYRGPPDGLATALAFGREYQVGTDLGPPLAFWLADIAYRAAGSHMFGIYLLSQFCIIVAFRVLFELARTIVGSQQAVLALLLTMTVTAFGAPSLTFGPQVLALPLWSLLLLHAWRIIGQGRNAWFALSIEAGLLLLTTPDAAGLLLALAVFALATERGRNALSSVDSLFALLVIAVLALPYLIWLVRADALGAPPWIDGLELNQLLRWGGLLLSLLLAIPGLVLLVVLNSRFVNRASEDAPVIVRPPVDPLAQIFVYVFALAPALGGSLVAALSGVDHVIGGAPIALSMSGLVIVLLSGDLIHLHRLRLLRAAWLTAVVAPALAVVAMVVGQPWFTNTEVATSLPASAIGAYFGESFERRTNRPLHAVAGDPQLAALVALGPSRPHLLLDAAPIAFSSEAESGSREENASKHKDSRRTPWITPAKFTELGGVVVWRASDTAGTPPEAIVKRFPGIVPEIPRTFDRLVNGRLPLLRIGWAIVRPKAP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPG4</accession>
    <name>CH603_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chaperonin GroEL 3</fullName>
            <ecNumber evidence="1">5.6.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">60 kDa chaperonin 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Chaperonin-60 3</fullName>
            <shortName evidence="1">Cpn60 3</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">groEL3</name>
        <name evidence="1" type="synonym">groL3</name>
        <name type="ordered locus">Nwi_2574</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Together with its co-chaperonin GroES, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + a folded polypeptide = ADP + phosphate + an unfolded polypeptide.</text>
            <dbReference type="EC" id="5.6.1.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a cylinder of 14 subunits composed of two heptameric rings stacked back-to-back. Interacts with the co-chaperonin GroES.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the chaperonin (HSP60) family.</text>
    </comment>
    <dbReference type="EC" id="5.6.1.7" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315778.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SPG4"/>
    <dbReference type="SMR" id="Q3SPG4"/>
    <dbReference type="STRING" id="323098.Nwi_2574"/>
    <dbReference type="PRIDE" id="Q3SPG4"/>
    <dbReference type="EnsemblBacteria" id="ABA05827">
        <property type="protein sequence ID" value="ABA05827"/>
        <property type="gene ID" value="Nwi_2574"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2574"/>
    <dbReference type="eggNOG" id="COG0459">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016503_3_0_5"/>
    <dbReference type="OMA" id="SSAMFDK"/>
    <dbReference type="OrthoDB" id="265347at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03344">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00600">
        <property type="entry name" value="CH60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018370">
        <property type="entry name" value="Chaperonin_Cpn60_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001844">
        <property type="entry name" value="Cpn60/GroEL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
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    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00298">
        <property type="entry name" value="CHAPERONIN60"/>
    </dbReference>
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        <property type="entry name" value="SSF48592"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="SSF52029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="SSF54849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02348">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00296">
        <property type="entry name" value="CHAPERONINS_CPN60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000256938" description="Chaperonin GroEL 3">
        <location>
            <begin position="1"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="30"/>
            <end position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="87"/>
            <end position="91"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="415"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="496"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00600"/>
        </source>
    </evidence>
    <sequence length="548" mass="57757" checksum="D22DA4B3455ABE7F" modified="2005-10-11" version="1">MAAKDVKFSGDARQRMLRGVDILADAVKVTLGPKGRNVVIERSFGAPRITKDGVTVAKEIELEDRFENMGAQMVREVASKTNDLAGDGTTTATVLAQAIVREGAKAVAAGMNPMDLKRGIDIAVAAVVKDIGKRAKAVASSAEVAQVGTISSNGDASIGKMIAQAMQKVGNDGVITVEENKSLETDVDIVEGMKFDRGYLSPYFVTNAEKMAAELDDAYILLHEKKLTGLQALLPVLEAVVKSGKPLLIVAEDVEGEALAALVVNRLRGGLKVAAVKAPGFGDRRKAMLEDIAILTSGQLISDELGMKLENVTLNMLGRAKKVLIDKENTTIVNGAGKKKDIEARVGQIRARIEETTSDYDREKLQERLAKLAGGVAVIRVGGATEIEVKEKKDRVEDALNATRAAVEEGIVPGGGTALLRARKAVGRINNDNSDVQAGINIVLKALEAPIRQIAENAGVEGSIVVGKILENKTETFGFDAQKEEYVDMVAKGIIDPAKVVRTALQDASSIAGLLVTTEAMVAELPKDEPPAMPAGGGGMGGMGGMGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPK7</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">Nwi_2531</name>
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    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05784.1"/>
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    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2531"/>
    <dbReference type="EnsemblBacteria" id="ABA05784">
        <property type="protein sequence ID" value="ABA05784"/>
        <property type="gene ID" value="Nwi_2531"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2531"/>
    <dbReference type="eggNOG" id="ENOG502ZX51">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2357852_0_0_5"/>
    <dbReference type="OMA" id="ACRWVPY"/>
    <dbReference type="OrthoDB" id="1934577at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229030" evidence="1">
        <location>
            <begin position="24"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="96" mass="10196" checksum="90C9A4D803F8FE28" modified="2005-10-11" version="1">MRLFGAALLAAMTGSLVFGPARADWVPPLKGNDTGGIISYSLAGASDVRVIAAHHCAHYGKVAKFLGVQAYPGGYVSFACRWVPYGPDQPPLQTRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPP6</accession>
    <name>Q3SPP6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2492</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
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            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_163853_0_0_5"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229103" evidence="2">
        <location>
            <begin position="30"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="122" mass="13466" checksum="0FC48E3D66A5E998" modified="2005-10-11" version="1">MRGPMNNRSLPLMLMMVPLCMPGSGAALAQDVPGIEICTVEKKMERRTSCLQSNINFLQTSLTRQVTDSSRKIEAANRQIQVLESTVADLRKAVAELQAASRKQPQSPVKEDKKTPAKQEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPQ9</accession>
    <name>Q3SPQ9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Lytic murein transglycosylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2479</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05732.1"/>
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    </dbReference>
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    <dbReference type="CAZy" id="GH103">
        <property type="family name" value="Glycoside Hydrolase Family 103"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05732">
        <property type="protein sequence ID" value="ABA05732"/>
        <property type="gene ID" value="Nwi_2479"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2479"/>
    <dbReference type="eggNOG" id="COG2951">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035402_0_2_5"/>
    <dbReference type="OMA" id="WAYEVRA"/>
    <dbReference type="OrthoDB" id="710313at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.101.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043426">
        <property type="entry name" value="MltB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011970">
        <property type="entry name" value="MltB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036366">
        <property type="entry name" value="PGBDSf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031304">
        <property type="entry name" value="SLT_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30163">
        <property type="entry name" value="PTHR30163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13406">
        <property type="entry name" value="SLT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="SSF47090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02283">
        <property type="entry name" value="MltB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229041" evidence="1">
        <location>
            <begin position="34"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="SLT_2" evidence="3">
        <location>
            <begin position="38"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="PG_binding_1" evidence="2">
        <location>
            <begin position="361"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="419" mass="45928" checksum="2EB10CE6FE3DC710" modified="2005-10-11" version="1">MAATAANILRRSQLAILILLTFAVGAAFSPVHAADEGFSRFIASVWPDAQQAGVSRATFDSVTAGLEPDHRLPDLILPGRPATGAPSQAEFVQVPADYLKEGSIARLAAEGQRLMRKHESTLKAIEKRFGVPATIILAIWGRETDYGRYALPYDAMRVLATQAYVGRRKDQYRNEFIEGLLLLQRGAVSRRNFRASWGGAVGLTQFLPSELAKHGVDFDGDGRVDIWSSVPDALASAAQQLVNKGWQPGLHWAYEVRAPAHADCTEGVPEVRKPIGEWLRAGFAPVRGQKLSAAEREQSASLLQPEGIHGPSFLTTKNYFVIKEYNFSDLYVLFVGHLADRMTSPLPFATPWSSSTQLRSRDVEAMQRHLTRLGLYKDKIDGKAGMLTRAALGAYQKSAGLKVDCWPSDGVLRAMNAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPV0</accession>
    <name>Q3SPV0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Lytic murein transglycosylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2438</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315646.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="CAZy" id="GH103">
        <property type="family name" value="Glycoside Hydrolase Family 103"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05691">
        <property type="protein sequence ID" value="ABA05691"/>
        <property type="gene ID" value="Nwi_2438"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2438"/>
    <dbReference type="eggNOG" id="COG2951">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035402_3_0_5"/>
    <dbReference type="OMA" id="ATMAHDC"/>
    <dbReference type="OrthoDB" id="710313at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043426">
        <property type="entry name" value="MltB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011970">
        <property type="entry name" value="MltB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031304">
        <property type="entry name" value="SLT_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30163">
        <property type="entry name" value="PTHR30163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13406">
        <property type="entry name" value="SLT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02283">
        <property type="entry name" value="MltB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229247" evidence="1">
        <location>
            <begin position="26"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="SLT_2" evidence="2">
        <location>
            <begin position="32"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="272" mass="29760" checksum="EE4CED0E01247DFA" modified="2005-10-11" version="1">MKIRFAVAFAAAVLAAPIASTAVSAAQCGGDFDTFVAEMSREAAANGVSQGVISQALGGVHQDQAVLSFDRRQRGTFRKSFEQYVATRVTGGRIKGGKAMLTRHGSLLSRIEKQFGVPPEIIVAIWGLETDFGRGDMGKLPVFRVLATMAHDCRRTELFQRELLAALRIVQRGDLRLSDMVGAYAGEIGQTQFLPSSYIKYGVDFDGNGHVDLRHSVPDVLASTANLLKVNGWKAGGDYHQGSPNFEVMREWNRSEIYRRTIGYFADKLAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPW0</accession>
    <name>Q3SPW0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2427</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2427"/>
    <dbReference type="EnsemblBacteria" id="ABA05681">
        <property type="protein sequence ID" value="ABA05681"/>
        <property type="gene ID" value="Nwi_2427"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2427"/>
    <dbReference type="HOGENOM" id="CLU_1738596_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="150" mass="17089" checksum="BD163351AA46E74C" modified="2005-10-11" version="1">MIFSEDRFPRFGIMLELRTLTSVDDKAASASRRPFNETGQVAGDATSRSRPVLVRRRPLPERVGCFIVPVATAQEHQKHQSASDDERKERAEPKRDPAVFSYLDTGRLIQDRDHPHAGGYQYARQRNQNDNSAFAHVFHRDAFPWLSLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPX7</accession>
    <name>Q3SPX7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">O-antigen polymerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2410</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the O-antigen polymerase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315624.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2410"/>
    <dbReference type="TCDB" id="9.B.67.6.8">
        <property type="family name" value="the o-antigen polymerase (oap) family"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05664">
        <property type="protein sequence ID" value="ABA05664"/>
        <property type="gene ID" value="Nwi_2410"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2410"/>
    <dbReference type="eggNOG" id="COG3307">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051481_0_0_5"/>
    <dbReference type="OMA" id="WRAISSH"/>
    <dbReference type="OrthoDB" id="754057at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007016">
        <property type="entry name" value="O-antigen_ligase-related"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04932">
        <property type="entry name" value="Wzy_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="245"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="340"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="370"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="398"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="426" mass="47678" checksum="8431D3C27AF9532F" modified="2005-10-11" version="1">MTFRFLINPIKEKRYVLRDSWISAAHRSRTADHLAVLMAISLPWSTTIFGVFTVLWLIMLLTVIDARQFRFQFRRPAYWLPVALFCLAAIGMLWADAPWAARIHAAGSVVKLLAIPLLMHQFERSGKGLAVFLAFLGSCTVLLALSWLSWFEPRVIPAHDKIPGVPVKNWITQGVEFVLCIFGSAALAIVIWRRQRRYAAAGLVLLGAAFFLNMALVASSRTALVSLPILLLISTFRYMKWREALILYTAIAITAALTWFLAPNLQERIASIQQQYASSQSQQTSVGLRLEYWTKSLRFIRNAPLAGHGTGSIRSLFEREAGGEAVTDNQIVANPHNQTLYFALEWGVIGVILLYAMWLSHFLMFIDMRWISWLGIIVVSQNVLGSLFNSHLSDYVEGWIYVMGVGVAGGMVSRVRKPPPVAGPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ47</accession>
    <name>Q3SQ47_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Substrate-binding region and inner membrane component of ABC-type glycine betaine transport system</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_2340</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315558.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2340"/>
    <dbReference type="EnsemblBacteria" id="ABA05594">
        <property type="protein sequence ID" value="ABA05594"/>
        <property type="gene ID" value="Nwi_2340"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2340"/>
    <dbReference type="eggNOG" id="COG1174">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1732">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038355_0_0_5"/>
    <dbReference type="OMA" id="FRSLHEM"/>
    <dbReference type="OrthoDB" id="770506at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007210">
        <property type="entry name" value="ABC_Gly_betaine_transp_sub-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04069">
        <property type="entry name" value="OpuAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="55"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="230"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="7">
        <location>
            <begin position="20"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA05594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="523" mass="56574" checksum="128772D58BCA5C1F" modified="2005-10-11" version="1">MSLLFDPRWSEALAHLPDYLGNHVRVSLTALALGLAISFPLALTARNRPMLRSALLGFASIVQTMPGLALLALFYPLLLALAIMTSQWFGFGFSAFGFLPSVLALALYSMLPVLRNTITGLQGIDPALTEAARGVGMTARQSLTMVELPLALPVIMAGIRTAAVWVIGTATLSTPIGQTSLGNYIFAGLQTLNWVFVLFGCIAAAALALVVDQLLALIESGLRMRRRIRVSTGIVGIAILVAATLAPSLTRATSTTIVGAKTFTEQYVLAALISHRLQAAGLPATTREGLGSNVIFDALANGDIDVYVDYSGTLWANQFHRTDLRPREELLTELETILARRNITLLGELGFENAYALLMPKARADALGIHTISDLAAHAGNMSIAGDYEFFARPEWTALKKAYGLSFRERRQMQSDFMYAAVASGEVDVIAGYTSDGLIAKYGLTVLDDTRHAIPPYDAIVLLAPRRANDAKLRAALQPLLGRIDIAIMREANFRASDSDTDNSPATVARWLWTKINEKKINE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ63</accession>
    <name>Q3SQ63_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Periplasmic binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2324</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2324"/>
    <dbReference type="EnsemblBacteria" id="ABA05578">
        <property type="protein sequence ID" value="ABA05578"/>
        <property type="gene ID" value="Nwi_2324"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2324"/>
    <dbReference type="eggNOG" id="COG4558">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_965878_0_0_5"/>
    <dbReference type="OMA" id="PNVGYMR"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="2">
        <location>
            <begin position="1"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="288" mass="30761" checksum="67B3B68ED9CCEB6A" modified="2005-10-11" version="1">MRMSNSSSTSKYKRGRLAQSQQRYQTPPKCTLKPALKTMPGPGLANRTSFWLVPSVGYMRQLSSEGVLGLNPSVIFAMQGSGPKETLDVLQAAKVPFVLVPEGYSEQGILDKISLVGHALRADARAACLTAAVAHDLTRLKGLRARIDKPVRVMFVMSLVNGRAMAAGRHTAADEIIKLSGGINAITEYEGYKPISDEAIVAAKPDVVLSIDRGNDSLAPDTIYQHPAFALTPAAANKSFISMEGLYLLGFGPRTAAAARDLSIRLYPALSSGTDNFTPAAPTVDCLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ85</accession>
    <name>Q3SQ85_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Siderophore synthetase component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2302</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IucA/IucC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2302"/>
    <dbReference type="EnsemblBacteria" id="ABA05556">
        <property type="protein sequence ID" value="ABA05556"/>
        <property type="gene ID" value="Nwi_2302"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2302"/>
    <dbReference type="eggNOG" id="COG4264">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018283_0_0_5"/>
    <dbReference type="OMA" id="TGWHRFG"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016881">
        <property type="term" value="F:acid-amino acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019290">
        <property type="term" value="P:siderophore biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007310">
        <property type="entry name" value="Aerobactin_biosyn_IucA/IucC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022770">
        <property type="entry name" value="FhuF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037455">
        <property type="entry name" value="LucA/IucC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34384">
        <property type="entry name" value="PTHR34384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06276">
        <property type="entry name" value="FhuF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04183">
        <property type="entry name" value="IucA_IucC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IucA_IucC" evidence="2">
        <location>
            <begin position="160"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="FhuF" evidence="3">
        <location>
            <begin position="416"/>
            <end position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="609" mass="67658" checksum="73D7831FA943CF15" modified="2005-10-11" version="1">MWPIDPGRFAEEYMLMQISKSQIAERATFQSFANCYLREVDPGVVSAHCLEDGVAVDCVEWDCAMTAARLRAEIERYSLCGPLRFGRIFVCRLPGNSWREIAPLRALYLMVQECYASMGAERADAMRNEEVELLGRVLTSYRCIADGLECAAAHRCGMSFLDGEQSLAFGHWLHPTPKSLQGMTSWQRPAYAPEHHGRFRLTFFAADATLLRHDAAPGVSLPDILRTLIPSRDCKLLRQGEALIPMHPLQAEALMLDPSIAQLVAQGRLRPLGAIGAWFTPTSSVRTVYNADLPWMLKFSLPVRITNSLRVNHMPELEAGVAMSRLFAGTDFLARYPRFRLIRDPAYLTLALPGRAESGFEVIFRENPFTKGSENSGVTIAALTADPPPGARSWLETIIRDLAARDGEPVREVCRRWFSAYLDCALEPLVRLYDELGVALEAHQQNSLLDVSSGYPDACYYRDNQGFYLSNTHRDRLTALAPETDGIPGLYFDDCVIQERFGYYLIVNQICSVIARMGHDGLIMEDALLAMLRARLERLASELTGVGGEFAAGFLSRPTIAAKANLLTRMLDIDELEADDGRAIYVQLPNPLCVASRAARQGGARAAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ94</accession>
    <name>Q3SQ94_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2293</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2293"/>
    <dbReference type="EnsemblBacteria" id="ABA05547">
        <property type="protein sequence ID" value="ABA05547"/>
        <property type="gene ID" value="Nwi_2293"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2293"/>
    <dbReference type="eggNOG" id="COG3553">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127482_1_1_5"/>
    <dbReference type="OMA" id="KLCKHYA"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014543">
        <property type="entry name" value="UCP028291"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09981">
        <property type="entry name" value="DUF2218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028291">
        <property type="entry name" value="UCP028291"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="100" mass="11306" checksum="36C653D8FB9A68A6" modified="2005-10-11" version="1">MNSQSRNAMETSRATVNTLHASRYLQQLCKHWAHKFETEYDSVNGRIALPLGEARLAAGPESLVIDLSTKDAASLATLREVVASHIERFAFRETLRFDWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQC0</accession>
    <name>Q3SQC0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Fe-S oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2267</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315488.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2267"/>
    <dbReference type="EnsemblBacteria" id="ABA05521">
        <property type="protein sequence ID" value="ABA05521"/>
        <property type="gene ID" value="Nwi_2267"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2267"/>
    <dbReference type="eggNOG" id="COG0535">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052502_0_0_5"/>
    <dbReference type="OMA" id="FNTDTPQ"/>
    <dbReference type="OrthoDB" id="1172757at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022563">
        <property type="entry name" value="DUF3463"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017833">
        <property type="entry name" value="Hopanoid_synth-assoc_rSAM_HpnH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11946">
        <property type="entry name" value="DUF3463"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00397">
        <property type="entry name" value="adenosyl-hopene_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03470">
        <property type="entry name" value="HpnH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="6">
        <location>
            <begin position="20"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="389" mass="43490" checksum="44894A624593F4D9" modified="2005-10-11" version="1">MAIPFFKELKISGYLIKQKLMGRKRYPLVLMLEPLFRCNLGCAGCGKIDYPDAILNRRMSAQECWDAAEECGAPMVAIPGGEPLIHKEIGEIVRGLVARKKFVSLCTNALLLEKKLDLFEPSPYLFFSVHLDGLREHHDKSVCQEGVFDRAVSAIKAAKARGFTVNVNATIFDNYPAEEIAKFLDFTAELGVGVSMSPGYAYERAPDQEHFLNRTKTKKLFRDVFALGKGKKWSFMHSSLFLDFLAGNQEYHCTPWGMPARNIFGWQKPCYLLGEGYTKTFKELMETTDWDSYGTGKYEKCANCMAHCGYEPTAATAALNNPLKTMWVALRGVRTTGPMAPEIDLSTQRPAQYIFAEQVQKKLSEIRKDEAAAAAAKQAAKEQKDSTAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQC5</accession>
    <name>Q3SQC5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Peptidase M20D, amidohydrolase</fullName>
            <ecNumber evidence="3">3.5.1.32</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2262</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.1.32" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315483.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2262"/>
    <dbReference type="EnsemblBacteria" id="ABA05516">
        <property type="protein sequence ID" value="ABA05516"/>
        <property type="gene ID" value="Nwi_2262"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2262"/>
    <dbReference type="eggNOG" id="COG1473">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023257_0_1_5"/>
    <dbReference type="OMA" id="RAHACGH"/>
    <dbReference type="OrthoDB" id="796259at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047980">
        <property type="term" value="F:hippurate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017439">
        <property type="entry name" value="Amidohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11014">
        <property type="entry name" value="PTHR11014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005962">
        <property type="entry name" value="Pept_M20D_amidohydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01891">
        <property type="entry name" value="amidohydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="M20_dimer" evidence="2">
        <location>
            <begin position="191"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="389" mass="41636" checksum="A22D112BE14873C8" modified="2005-10-11" version="1">MPVVNRVADLQSDIAGWRRDIHAHPELLYDVPRTAAFVADRLREFGCDEIVTGLGSTGVVGVIKGRRPAGGEVKVIGLRADMDALPIQEETNLPYASATAGRMHACGHDGHTAMLLGAARYLAETRNFAGEAIVIFQPAEEGGAGAAAMIKDGLMDRFRIDQVYGMHNLPGVPLGAFAIRPGPIMAATDSVDFRIEGHGGHAARPHLCIDPVLAGSQLVMALQSIVSRNVDPLESAVISICEFRAGSTRNVIPQTVELRGTVRALKADVRDQMEKRIREVAAGVALTTGANIDVKFRRGYPVTVNHPEQTETAIRIAKEIAGDGNVHGTPPMMGGEDFAYMLEARPGAFIFIGNGDSAGLHHPAYNFNDDAIAYGSSYWIRLVETTLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQI7</accession>
    <name>Q3SQI7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted sulfurylase large subunit, molybdopterin cytosine dinucleotide biosynthesis</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315423.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2200"/>
    <dbReference type="EnsemblBacteria" id="ABA05454">
        <property type="protein sequence ID" value="ABA05454"/>
        <property type="gene ID" value="Nwi_2200"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2200"/>
    <dbReference type="eggNOG" id="COG1975">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041115_3_2_5"/>
    <dbReference type="OMA" id="RDCFYIG"/>
    <dbReference type="OrthoDB" id="1879870at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027051">
        <property type="entry name" value="XdhC_Rossmann_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13478">
        <property type="entry name" value="XdhC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XdhC_C" evidence="1">
        <location>
            <begin position="77"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="230" mass="24736" checksum="76ECDF9814EE3C8F" modified="2005-10-11" version="1">MNLATLSAINAERAARRPVIVVTDIGNGAQRLVKAVDIARDPLHIELERHLRMGKSSVIEVEGRKLFLNVYAPTAKLVIIGAVHISQALAPLARSLGYDVTVVDPRSAFASPARFPDVRLIAEWPDVALPPLNIDHYTAFVALTHDPKIDDPALLHALERDCFYIGALGSRNTHGQRAVRLKAKGVSEAGIERIHAPIGLEIGAITPAEIAVAIMAEITAQLRLPKASAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQM7</accession>
    <name>Q3SQM7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Thiolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2160</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315383.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2160"/>
    <dbReference type="EnsemblBacteria" id="ABA05414">
        <property type="protein sequence ID" value="ABA05414"/>
        <property type="gene ID" value="Nwi_2160"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2160"/>
    <dbReference type="eggNOG" id="COG0183">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035425_4_0_5"/>
    <dbReference type="OMA" id="QHAFYDV"/>
    <dbReference type="OrthoDB" id="311458at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase_N" evidence="2">
        <location>
            <begin position="5"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase_C" evidence="3">
        <location>
            <begin position="260"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="389" mass="40914" checksum="B28EB090965B36FB" modified="2005-10-11" version="1">MSASIVGWAHTPFGKLDTETVESLVVKAATDALADAGVSVGDVDEIVLGHFNAGFSPQDFTAALVLQAAPELRFKPATRVENACATGSAAVHQGIRAIASGAARIVLVVGVEQMTRTAGPEIGRNLLRASYLPEDGDTVGGFAGIFGKIAQAYFQKYGDASDALAMIAAKNHRNGVANPYAQMRKDVGYDFCRSESEKNPFVAGPLKRTDCSLVSDGAAALVLTDADTARSARKAVSIRGTGHAQDFLPMSRRDILAFEGCTVAWRRALAKAGVTLSDLSFVETHDCFTIAELIEYEAMGLTPKGQGARAIKEGWTQKDGRLPVNPSGGLKAKGHPIGATGVSMHVMSAMQLAGEAPEGMQIRDARLAGIFNMGGTAVANYVSILEPFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQS4</accession>
    <name>Q3SQS4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase, group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2112</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315340.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2112"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05367">
        <property type="protein sequence ID" value="ABA05367"/>
        <property type="gene ID" value="Nwi_2112"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2112"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042257_1_0_5"/>
    <dbReference type="OMA" id="PRHYGPW"/>
    <dbReference type="OrthoDB" id="694191at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="2">
        <location>
            <begin position="18"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="174"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="365" mass="40912" checksum="6DDD6DE79F9BEBCF" modified="2005-10-11" version="1">MKIAQIAPLAECCPPKLYGGTERIVSYLTEELVRQGHEVTLFASGDSRTQAKLVRCSDMALRLNPTVRDHTPYHVMMLDSVRGEADEFDVLHFHIDLLHFPLIRDIADRTVTTLHGRLDLPDLKPFYAAFPEIPLVSISRDQRRPLARPINWVGNVYHGLPRDLLPFSANPMGDYLAFLGRISPEKRPDRAIQIAARAGMKLKIAAKIDRADQEYWDDTIAPMIATHRNVEYIGEINEAQKADFLGNAKALLFPIDWPEPFGLVMIEAMACGTPVVAFRCGSVPEVVNAGKSGFIVRTLEEAVAALAKLETLDRQKVRDTFEKRFTVERMAQDYLAIYRGLPGVRTEAARIRRLQGDEVGLQAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQY5</accession>
    <name>Q3SQY5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Fec I like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2048</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05306">
        <property type="protein sequence ID" value="ABA05306"/>
        <property type="gene ID" value="Nwi_2048"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2048"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1617281_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="1">
        <location>
            <begin position="38"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="164" mass="17837" checksum="CE7BD6E4FE6592CE" modified="2005-10-11" version="1">MTKRPSLSRPSFSRLLNVLMTDRAGVENAVRGRTGEVASADIVQETWLKLANHPSDSAIENPSAFVRRVAKNSATDYLRKERRRSALNTEVHDLLYDTEDELSPERLVIGRQAAECATPSIHCPSSDELRLFRGHGNGGSGSSGFEVRRPAGDAASVFGGWSGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQY6</accession>
    <name>Q3SQY6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Helix-turn-helix, Fis-type</fullName>
            <ecNumber evidence="3">2.7.7.49</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1492</name>
        <name evidence="3" type="ordered locus">Nwi_2047</name>
        <name evidence="4" type="ordered locus">Nwi_2358</name>
        <name evidence="5" type="ordered locus">Nwi_2674</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="3 6" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="3">Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04753">
        <property type="protein sequence ID" value="ABA04753"/>
        <property type="gene ID" value="Nwi_1492"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05305">
        <property type="protein sequence ID" value="ABA05305"/>
        <property type="gene ID" value="Nwi_2047"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05612">
        <property type="protein sequence ID" value="ABA05612"/>
        <property type="gene ID" value="Nwi_2358"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05927">
        <property type="protein sequence ID" value="ABA05927"/>
        <property type="gene ID" value="Nwi_2674"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1492"/>
    <dbReference type="KEGG" id="nwi:Nwi_2047"/>
    <dbReference type="KEGG" id="nwi:Nwi_2358"/>
    <dbReference type="KEGG" id="nwi:Nwi_2674"/>
    <dbReference type="eggNOG" id="COG2801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2963">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027402_10_0_5"/>
    <dbReference type="OMA" id="GFVAWYN"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010921">
        <property type="entry name" value="Trp_repressor/repl_initiator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48295">
        <property type="entry name" value="SSF48295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="132"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="326" mass="37772" checksum="A5ED20ECF4BAC3F3" modified="2005-10-11" version="1">MRYPASEKAEIIRLVEASHLPARRTLDKLGIPRATFYRWYDRYLTGGIEALADHRSRPDRVWNRIPDPIRAEIVELALRETELSPRELAVRFTDEKRYFVSEASVYRLLKAHDLITSPAYIVIKAASEFKDKTTAPNQLWQTDFTYLKITGWGWYYLSTVLDDFSRFIVAWKLCATMRADDVTATLDLALAASGLDQIAVAHRPRLLSDNGASYISAELATWLDGKGMKHVRGAPYHPQTQGKIERWHQTLKNRILLENYYLPGDLERQVAAFVEHYNHGRYHESIDNLTPADVYFGRGQTILTERERIKRQTIHQRRLQHHLQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="137" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQZ7</accession>
    <name>Q3SQZ7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Threonine--tRNA ligase</fullName>
            <ecNumber evidence="13">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Threonyl-tRNA synthetase</fullName>
            <shortName evidence="13">ThrRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">thrS</name>
        <name evidence="16" type="ordered locus">Nwi_2035</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + H(+) + L-threonyl-tRNA(Thr)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 13">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="13"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315278.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2035"/>
    <dbReference type="EnsemblBacteria" id="ABA05294">
        <property type="protein sequence ID" value="ABA05294"/>
        <property type="gene ID" value="Nwi_2035"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2035"/>
    <dbReference type="eggNOG" id="COG0441">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008554_0_1_5"/>
    <dbReference type="OMA" id="FYYDFAY"/>
    <dbReference type="OrthoDB" id="900765at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004829">
        <property type="term" value="F:threonine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006435">
        <property type="term" value="P:threonyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00771">
        <property type="entry name" value="ThrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00184">
        <property type="entry name" value="Thr_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033728">
        <property type="entry name" value="ThrRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="PTHR11451"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01047">
        <property type="entry name" value="TRNASYNTHTHR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="SSF55186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="SSF81271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00418">
        <property type="entry name" value="thrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 13" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="8 13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="TGS" evidence="15">
        <location>
            <begin position="16"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="14">
        <location>
            <begin position="267"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="414"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="546"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ABA05294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="686" mass="76988" checksum="13803CEF6118DE69" modified="2005-10-11" version="1">MSENQDPAGFRYGLANLKPVSPADNVVLTFPDGATREFPKNTTGLEIAKGISPSLAKRTVAMALDGIVNDLADPIEQDATIEFISRDDPRALELIRHDAAHVLAEAVQTLWPGTQVTIGPVIENGFYYDFFRNEPFSVEDFAAIEKKMREIIARDQPFTKDVWDREKTKQVFRDKGEAFKVELVDAIPGNEPIRIYYQGDWFDLCRGPHMTSTGKIGNAFKLMKVAGAYWRGDSNNPMLTRIYGTAFANQADLDAYLKQIEEAEKRDHRKLGRELDLFHFQEEGPGVVFWHAKGWTIFQAVIAYMRRRLAEDYDEVNAPQMLDKSLWETSGHWEWYRENMFAAQSAGDEAEDKRWFTIKPMNCPGHVQLFKHGLKSYRDLPLRLAEFGVVHRYEPSGAMHGLMRVRGFTQDDAHVFCTEEQLADECLKINELILSTYADFGFDGELTVKLSTRPEKRVGSDAAWDHAEAVMADVLKHIAASNNRIRTETNPGEGAFYGPKFEYVLRDAIGRDWQCGTTQVDFNLPERFGAFYIDADGGKKAPVMVHRAICGSIERFIGILIEHFAGHFPLWLAPVQAVVTTITSDGDEYAKVVASAARRAGLRVELDLRNEKINYKVREHSLAKIPALLVVGKKEAETQSVSIRRLGSDGQKVTPTGEAIAALVAEAVPPDIQRIKTAPQSLSASD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR43</accession>
    <name>Q3SR43_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nitrogen regulatory protein P-II</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_1989</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">In nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP. P-II-UMP allows the deadenylation of glutamine synthetase (GS), thus activating the enzyme. Conversely, in nitrogen excess P-II is deuridylated and promotes the adenylation of GS. P-II indirectly controls the transcription of the GS gene (glnA). P-II prevents NR-II-catalyzed conversion of NR-I to NR-I-phosphate, the transcriptional activator of glnA. When P-II is uridylylated to P-II-UMP, these events are reversed.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotrimer.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315235.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1989"/>
    <dbReference type="EnsemblBacteria" id="ABA05248">
        <property type="protein sequence ID" value="ABA05248"/>
        <property type="gene ID" value="Nwi_1989"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1989"/>
    <dbReference type="eggNOG" id="COG0347">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_173795_0_0_5"/>
    <dbReference type="OMA" id="FHEAHYM"/>
    <dbReference type="OrthoDB" id="1864676at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002187">
        <property type="entry name" value="N-reg_PII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51343">
        <property type="entry name" value="PII_GLNB_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="108" mass="11936" checksum="BF7E2629F94FF864" modified="2005-10-11" version="1">MNEIKLHPLKKLEIILQGEHREFATDLLDRAGVKGYTIIGNLSGKGAHGMYEGHLMFNEDDVLIMIIAAVPPSLFDSLLEGFLPFFEAHSGVILVSDIQVGRLVKFMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR50</accession>
    <name>Q3SR50_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ethanolamine utilization protein EutN/carboxysome structural protein Ccml</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1982</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1982"/>
    <dbReference type="EnsemblBacteria" id="ABA05241">
        <property type="protein sequence ID" value="ABA05241"/>
        <property type="gene ID" value="Nwi_1982"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1982"/>
    <dbReference type="eggNOG" id="COG4576">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148498_1_0_5"/>
    <dbReference type="OMA" id="RHACPDN"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031469">
        <property type="term" value="C:bacterial microcompartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01614">
        <property type="entry name" value="EutN_CcmL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014077">
        <property type="entry name" value="CsoS4B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004992">
        <property type="entry name" value="EutN_CcmL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036677">
        <property type="entry name" value="EutN_CcmL_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03319">
        <property type="entry name" value="EutN_CcmL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159133">
        <property type="entry name" value="SSF159133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02704">
        <property type="entry name" value="carboxysome_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51932">
        <property type="entry name" value="BMV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="84" mass="8989" checksum="C2E5186533C9A4E1" modified="2005-10-11" version="1">MMEVWRVVDELVCTRRVPEMGSASLRILKGQKGDISVATDPVGAPPGKWVFTAAGTAARMAMPDRTVATDLAICGIIDGWEEEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR84</accession>
    <name>Q3SR84_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable phosphoketolase</fullName>
            <ecNumber evidence="5">4.1.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_1947</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the XFP family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.-" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315198.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1947"/>
    <dbReference type="EnsemblBacteria" id="ABA05207">
        <property type="protein sequence ID" value="ABA05207"/>
        <property type="gene ID" value="Nwi_1947"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1947"/>
    <dbReference type="eggNOG" id="COG3957">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013954_2_0_5"/>
    <dbReference type="OMA" id="LSTMDHC"/>
    <dbReference type="OrthoDB" id="45239at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016832">
        <property type="term" value="F:aldehyde-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01403">
        <property type="entry name" value="Phosphoketolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023962">
        <property type="entry name" value="Phosphoketolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005593">
        <property type="entry name" value="Xul5P/Fru6P_PKetolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018969">
        <property type="entry name" value="Xul5P/Fru6P_PKetolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019790">
        <property type="entry name" value="Xul5P/Fru6P_PKetolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018970">
        <property type="entry name" value="Xul5P/Fru6P_PKetolase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019789">
        <property type="entry name" value="Xul5P/Fru6P_PKetolase_ThDP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31273">
        <property type="entry name" value="PTHR31273"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03894">
        <property type="entry name" value="XFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09363">
        <property type="entry name" value="XFP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09364">
        <property type="entry name" value="XFP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017245">
        <property type="entry name" value="Phosphoketolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS60002">
        <property type="entry name" value="PHOSPHOKETOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS60003">
        <property type="entry name" value="PHOSPHOKETOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="XFP_N" evidence="7">
        <location>
            <begin position="5"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="XFP_C" evidence="6">
        <location>
            <begin position="583"/>
            <end position="785"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA05207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="795" mass="89477" checksum="C5A0808D8FE856D5" modified="2005-10-11" version="1">MAELDDTQLGLMNLYWRAANYVSVGQIYLMDNPLLRKPLKAEDVKPRLLGHWGTTPGLNFIYVHLNRVIRERGTDVLYICGPGHGGPAMVANTYLEGSYSEIYPGITQDEAGLQKLFRQFSFPGGVPSHVAPETPGSIHEGGELGYALVHAFGAAFDNPDLTVACVVGDGEAETGPLAAAWHSNRFLNPQVDGAVLPILHLNGYKIANPALLARMSEAELSDLFRGYGYEPLFVEGHEPIPMHRTMAESLDLAMDRIRDIQREARKEGWSGERPRWPMIILRSPKGWTGPKEVDGKKVEDFWRSHQVPVSNARGDATHRKILEEWLRSYRPQELFDGKGRFLPEIAALAPEGAKRMGALPHANGGLLKKDLILPDWKSLALDIGQPGETIAEATRLMGAYLREVIRLNAEARNFRLMGPDETASNRLDAVFEVTNRVWMEKIESYDVHLACEGRVMEVLSEHLCQGWLEGYLLTGRHGVFSCYEAFIHIVDSMVNQHAKWLKVSAELPWRKPIASLNYLLTSHVWRQDHNGFSHQDPGFADFVANKKADIVRLYFPPDANTLLWVTDHCLRTWNRINVITAGKQPQPQWLTAEQAERHCEAGAGIWEWACTCPADEEPDVVMACCGDVPTLEILAAVGLLRRELPDLRIRVVNVVDLMTLQSHTTHPHGFTDDEFDALFTKEKPVIFAYHGYPYLIHRLTYKRTNHANFHVHGFQEEGTTTTPFDMAVMNELDRFHLVIAAVRRLPNLGMGGERVIGRCEQALAEHASYARQYGEDMPEIREWSWPYKTAAEAGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR85</accession>
    <name>Q3SR85_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable transmembrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1946</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315197.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1946"/>
    <dbReference type="EnsemblBacteria" id="ABA05206">
        <property type="protein sequence ID" value="ABA05206"/>
        <property type="gene ID" value="Nwi_1946"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1946"/>
    <dbReference type="eggNOG" id="ENOG5032KYR">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_182795_0_0_5"/>
    <dbReference type="OMA" id="AWQTTIK"/>
    <dbReference type="OrthoDB" id="1986017at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046130">
        <property type="entry name" value="DUF6127"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19622">
        <property type="entry name" value="DUF6127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="95" mass="10263" checksum="AA64BBC6AB83AF6F" modified="2005-10-11" version="1">MTPPEANEQFYMSETEFEALLARAAETGARRALQEVGLEGKDAAEDIRDLRSLLAGFRLAKRTAVQTTVRIITTGILIALMAGIAIKLKIFGGSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRF6</accession>
    <name>Q3SRF6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NADH dehydrogenase subunit M</fullName>
            <ecNumber evidence="7">1.6.5.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_1875</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 3">Membrane</location>
            <topology evidence="1 3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I subunit 4 family.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.3" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315131.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1875"/>
    <dbReference type="EnsemblBacteria" id="ABA05135">
        <property type="protein sequence ID" value="ABA05135"/>
        <property type="gene ID" value="Nwi_1875"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1875"/>
    <dbReference type="eggNOG" id="COG1008">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007100_4_4_5"/>
    <dbReference type="OMA" id="ITRWGNQ"/>
    <dbReference type="OrthoDB" id="535707at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000260">
        <property type="entry name" value="NADH4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010227">
        <property type="entry name" value="NADH_Q_OxRdtase_chainM/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003918">
        <property type="entry name" value="NADH_UbQ_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_mem"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43507">
        <property type="entry name" value="PTHR43507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01059">
        <property type="entry name" value="Oxidored_q5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01437">
        <property type="entry name" value="NUOXDRDTASE4"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01972">
        <property type="entry name" value="NDH_I_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="212"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="246"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="276"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="304"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="331"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="373"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="407"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="451"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="Oxidored_q5_N" evidence="6">
        <location>
            <begin position="44"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Proton_antipo_M" evidence="5">
        <location>
            <begin position="134"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="502" mass="54721" checksum="CD7E80C18A579226" modified="2005-10-11" version="1">MTTWPILSVTTFLPAVGAILIYVMARGSDEAANRTARWIALWTTIVTFAVSLLLVTRFDPTNTGFQFVEQATWLATGIRYHMGVDGISLPFVILTTALMPLCIIASWKSITRRVPAYMMAFLVLETLMIGTFSALDLLLFYVFFEGGLIPMFLIIGIWGGPRRVYATFKFFLYTLLGSVLMLLAIMALYWNAGTTDIPTLMQTSVPYNLQKWAWLAFFASFAVKMPMWPVHTWLPDAHVEAPTAGSVILAAIMLKMGGYGFLRFSLPMFPLASHDFAPLVFVLSAVAIVYTSLVALMQEDMKKLIAYSSVAHMGFVTMGIFAGTMQGIHGGVFQMVSHGIVSGALFLCVGVVYDRMHTREIAAYGGLVNRMPLYALVFLIFTMANVGLPGTSGFVGEFLALIGTFKVSIPTAVAATLGVILSAAYALWLYRKIVFGALVKPSLASIKDLTFREGLTLAPLVVLTILFGVYPKPVLDFSAASVQNLVNNYASATAAIKAAALQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRI2</accession>
    <name>FABZ_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ</fullName>
            <ecNumber evidence="1">4.2.1.59</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">(3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase</fullName>
            <shortName evidence="1">(3R)-hydroxymyristoyl-ACP dehydrase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Beta-hydroxyacyl-ACP dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fabZ</name>
        <name type="ordered locus">Nwi_1849</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in unsaturated fatty acids biosynthesis. Catalyzes the dehydration of short chain beta-hydroxyacyl-ACPs and long chain saturated and unsaturated beta-hydroxyacyl-ACPs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:13097"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="4.2.1.59"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioester dehydratase family. FabZ subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.59" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315105.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRI2"/>
    <dbReference type="SMR" id="Q3SRI2"/>
    <dbReference type="STRING" id="323098.Nwi_1849"/>
    <dbReference type="EnsemblBacteria" id="ABA05109">
        <property type="protein sequence ID" value="ABA05109"/>
        <property type="gene ID" value="Nwi_1849"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1849"/>
    <dbReference type="eggNOG" id="COG0764">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078912_1_0_5"/>
    <dbReference type="OMA" id="HFPHEPV"/>
    <dbReference type="OrthoDB" id="1763197at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008659">
        <property type="term" value="F:(3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008693">
        <property type="term" value="F:3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047451">
        <property type="term" value="F:3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004317">
        <property type="term" value="F:3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009245">
        <property type="term" value="P:lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00406">
        <property type="entry name" value="FabZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013114">
        <property type="entry name" value="FabA_FabZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010084">
        <property type="entry name" value="FabZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30272">
        <property type="entry name" value="PTHR30272"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07977">
        <property type="entry name" value="FabA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01750">
        <property type="entry name" value="fabZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0441">Lipid A biosynthesis</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000230819" description="3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00406"/>
        </source>
    </evidence>
    <sequence length="151" mass="17011" checksum="971562FDB20CA383" modified="2005-10-11" version="1">MESPVRFEHVDIATILKTLPHRYPFLLIDRVLNIRADHSGIGVKNVTFNEPAFQGHFPERPVYPGVLMIEGMAQTAGVIGIMSVEGTEKPRAVYFLTIDKCKFRKPVMPGDIVEYHMRSIGRRKTMWWFHGDAKVNGTIVAEADVGAMLTD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-09-11" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRK0</accession>
    <name>KDSA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">2-dehydro-3-deoxyphosphooctonate aldolase</fullName>
            <ecNumber evidence="1">2.5.1.55</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">3-deoxy-D-manno-octulosonic acid 8-phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">KDO-8-phosphate synthase</fullName>
            <shortName evidence="1">KDO 8-P synthase</shortName>
            <shortName evidence="1">KDOPS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phospho-2-dehydro-3-deoxyoctonate aldolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">kdsA</name>
        <name type="ordered locus">Nwi_1831</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-arabinose 5-phosphate + H2O + phosphoenolpyruvate = 3-deoxy-alpha-D-manno-2-octulosonate-8-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14053"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57693"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:85985"/>
            <dbReference type="EC" id="2.5.1.55"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate biosynthesis; 3-deoxy-D-manno-octulosonate biosynthesis; 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate: step 2/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the KdsA family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.55" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315087.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRK0"/>
    <dbReference type="SMR" id="Q3SRK0"/>
    <dbReference type="STRING" id="323098.Nwi_1831"/>
    <dbReference type="EnsemblBacteria" id="ABA05091">
        <property type="protein sequence ID" value="ABA05091"/>
        <property type="gene ID" value="Nwi_1831"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1831"/>
    <dbReference type="eggNOG" id="COG2877">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036666_0_0_5"/>
    <dbReference type="OMA" id="FRGIPTM"/>
    <dbReference type="OrthoDB" id="687380at2"/>
    <dbReference type="UniPathway" id="UPA00030"/>
    <dbReference type="UniPathway" id="UPA00357">
        <property type="reaction ID" value="UER00474"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008676">
        <property type="term" value="F:3-deoxy-8-phosphooctulonate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019294">
        <property type="term" value="P:keto-3-deoxy-D-manno-octulosonic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00056">
        <property type="entry name" value="KDO8P_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006218">
        <property type="entry name" value="DAHP1/KDSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006269">
        <property type="entry name" value="KDO8P_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21057">
        <property type="entry name" value="PTHR21057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00793">
        <property type="entry name" value="DAHP_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01362">
        <property type="entry name" value="KDO8P_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0448">Lipopolysaccharide biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000304463" description="2-dehydro-3-deoxyphosphooctonate aldolase">
        <location>
            <begin position="1"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00056"/>
        </source>
    </evidence>
    <sequence length="287" mass="29822" checksum="606089DCE904CDBC" modified="2005-10-11" version="1">MNQPVSASPVVSVGSVTFGQDRPLSIIAGPCQMESRAHALEVAGALKDIAARLNVGLVFKTSFDKANRTSASGARGIGLKQALPVFADIRSSLGLPVLTDVHEAAQCAEVAQVVDVLQIPAFLCRQTDLLLAAAATGKVVNVKKGQFLAPWDMGNVVAKITGGGNRNILVTERGASFGYNTLVSDMRALPILARTTGAPVIFDATHSVQQPGGKGASTGGEREFVPVLARAAVAVGVAGVFIETHPDPDHAPSDGPNMVPLREFEALVRRLMAFDALAKAADPACPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRK7</accession>
    <name>Q3SRK7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1823</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315080.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1823"/>
    <dbReference type="EnsemblBacteria" id="ABA05084">
        <property type="protein sequence ID" value="ABA05084"/>
        <property type="gene ID" value="Nwi_1823"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1823"/>
    <dbReference type="eggNOG" id="COG3319">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084405_0_0_5"/>
    <dbReference type="OMA" id="PRSHGHL"/>
    <dbReference type="OrthoDB" id="1201878at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="224" mass="24418" checksum="BB15D0ADBAEEE054" modified="2005-10-11" version="1">MPTGFPPAQRGLRAGIGGLILFALGGFLTTSALARSSTPHRPGHVYLMRGLFNVFSLGMDDLAAKLRKVGVKAEAVSYTSWSSLADSITADFRAGNREPIILMGHSYGADTTISLAHKLNQDRIPVALIVNFDPTQPDRVPPNVRRIINFYVPTDWGRPVEPGKKFRGRLSNLDESGKHGHFEIDKADELHRKAIAAVLQAVGRGAPRIKRPAEHDQVSEAPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRL4</accession>
    <name>Q3SRL4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Acetyltransferase component of pyruvate dehydrogenase complex</fullName>
            <ecNumber evidence="7">2.3.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_1816</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>(R)-N(6)-dihydrolipoyl-L-lysyl-[protein] + acetyl-CoA = (R)-N(6)-(S(8)-acetyldihydrolipoyl)-L-lysyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:17017"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:10478"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="2.3.1.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
        <text evidence="7">Binds 1 lipoyl cofactor covalently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Forms a 24-polypeptide structural core with octahedral symmetry.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.12" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315073.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1816"/>
    <dbReference type="EnsemblBacteria" id="ABA05077">
        <property type="protein sequence ID" value="ABA05077"/>
        <property type="gene ID" value="Nwi_1816"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1816"/>
    <dbReference type="eggNOG" id="COG0508">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016733_10_2_5"/>
    <dbReference type="OMA" id="QVTVIKH"/>
    <dbReference type="OrthoDB" id="1626282at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043231">
        <property type="term" value="C:intracellular membrane-bounded organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045254">
        <property type="term" value="C:pyruvate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004742">
        <property type="term" value="F:dihydrolipoyllysine-residue acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:acetyl-CoA biosynthetic process from pyruvate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045257">
        <property type="entry name" value="E2/Pdx1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036625">
        <property type="entry name" value="E3-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006257">
        <property type="entry name" value="LAT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004167">
        <property type="entry name" value="PSBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23151">
        <property type="entry name" value="PTHR23151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02817">
        <property type="entry name" value="E3_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47005">
        <property type="entry name" value="SSF47005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01349">
        <property type="entry name" value="PDHac_trf_mito"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51826">
        <property type="entry name" value="PSBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="4 7" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="9">
        <location>
            <begin position="2"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="Peripheral subunit-binding (PSBD)" evidence="10">
        <location>
            <begin position="147"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="88"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA05077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="452" mass="47225" checksum="E9AB5712A78AF1DB" modified="2005-10-11" version="1">MPINILMPALSPTMEKGNLARWLKKEGDAVKSGDVIAEIETDKATMEVEAVDEGTIAKILVPEGTQDVPVNNVIAVLAGDGEDVKAAASGATAAPGNEAKPESRADAKAGSGEAAGSPEPSSRAPASKPASSGSQAAQPANGHARIFSSPLARRLASEAGIDLARIEGSGPHGRIVARDVEQAKSGKGLKAAAAPAPAGAPSIAPSMSDKQILSLFEDGSYELVPHDNMRRTIAQRLTASIQNVPHFYLTMDCDIGRLLSAREEINASAPKDKEKKPLYKLSVNDFVIKAMAVALQRVPNANVSWTEGGMLKHKHSDVGVAVAMPGGLITPIIRKAETKTLSAISSEMKDFAARARARKLKPEEYQGGTTAVSNLGMYGIKDFTAVINPPHATILAVGASEERAVVRGGRIEAAQIMSVTLSCDHRAVDGALGAELIGAFKTLIENPVMMMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRP2</accession>
    <name>Q3SRP2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1788</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315045.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05049">
        <property type="protein sequence ID" value="ABA05049"/>
        <property type="gene ID" value="Nwi_1788"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1788"/>
    <dbReference type="eggNOG" id="ENOG5033Z7N">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118007_0_0_5"/>
    <dbReference type="OMA" id="CWINPDA"/>
    <dbReference type="OrthoDB" id="1627920at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="190" mass="20181" checksum="21B6F862DC6F03B9" modified="2005-10-11" version="1">MRRMLFGFEKALWRGLAVPGFALLFGIGGAIAQSGDLRAQDQNKPAAENGTNGADGAKDSQRKTDEFVEAARVINGPAGNPECVWLGRRVVVLMWRDDLDTAFRHLDLYDRFGCPGGHVQAAFRCLVRFGPIDPKAPDALSGRVHACWINPDAQPSGQDAAASPPAAHSSPAPATPAPQPAPAPPVPKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRR2</accession>
    <name>Q3SRR2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein-export membrane protein SecF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">secF</name>
        <name evidence="11" type="ordered locus">Nwi_1768</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Forms a complex with SecD. Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF-YajC and YidC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell inner membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SecD/SecF family. SecF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315025.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1768"/>
    <dbReference type="EnsemblBacteria" id="ABA05029">
        <property type="protein sequence ID" value="ABA05029"/>
        <property type="gene ID" value="Nwi_1768"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1768"/>
    <dbReference type="eggNOG" id="COG0341">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050012_1_1_5"/>
    <dbReference type="OMA" id="AFEWRMA"/>
    <dbReference type="OrthoDB" id="410730at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015450">
        <property type="term" value="F:protein-transporting ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043952">
        <property type="term" value="P:protein transport by the Sec complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01464_B">
        <property type="entry name" value="SecF_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022813">
        <property type="entry name" value="SecD/SecF_arch_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022645">
        <property type="entry name" value="SecD/SecF_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022646">
        <property type="entry name" value="SecD/SecF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005665">
        <property type="entry name" value="SecF_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30081">
        <property type="entry name" value="PTHR30081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07549">
        <property type="entry name" value="Sec_GG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02355">
        <property type="entry name" value="SecD_SecF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01755">
        <property type="entry name" value="SECFTRNLCASE"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00916">
        <property type="entry name" value="2A0604s01"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00966">
        <property type="entry name" value="3a0501s07"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="215"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="267"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="294"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="328"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA05029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="352" mass="38337" checksum="42A15086E3C1F694" modified="2005-10-11" version="1">MTQWILIGLGALIVVLTIVSCFGLLPSLRIIPDDTKFDFTRFRRISFPISAVLSIVAITLFFTHGLNFGIDFKGGTLLEVQAKSGEVDIASMRATLSKLDLGEVQLQQFGGPTDVLIRVAEQPGGDAAQQEAVQKVRGALGETVNYRRVEVVGPRVSGELLAYGMIGLILAIIGILVYLWFRFEWQFALGAMIANVHDIVLTIGFMSISQVDFDLTSIAALLTILGYSLNDTVVIYDRIREILRRYKKMPMPELLNASINSTLSRSIITHVTVTLALLALLLFGGPAIHSFTAVMTFGVVLVGTYTSIFIAAPILIYLGVGESRADVEEKPAKPEKPVKSEKLAKSAKPAKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRU3</accession>
    <name>Q3SRU3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1737</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314994.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04998">
        <property type="protein sequence ID" value="ABA04998"/>
        <property type="gene ID" value="Nwi_1737"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1737"/>
    <dbReference type="eggNOG" id="ENOG502ZB84">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090654_0_0_5"/>
    <dbReference type="OMA" id="TEVLEWI"/>
    <dbReference type="OrthoDB" id="1410643at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="190" mass="19936" checksum="919C469424D0CA90" modified="2005-10-11" version="1">MLSDLSAAVAPIDTFAISSRKDVVAALATVRNGVITGEGPTAGGRVHFSRALDSTDTSWCERLLTAAAVDNQPVSREEAEALFEINDAATERSDGGRFDDLFAKAIVHHAASASGLPVPSRSIALAPDTAIESWAPTRAVGVNIEVLEWIANQMRGKRRGNRQLMALVASLIGAATLPLAHLPGVLDLGM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRY8</accession>
    <name>RS18_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsR</name>
        <name type="ordered locus">Nwi_1692</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds as a heterodimer with protein S6 to the central domain of the 16S rRNA, where it helps stabilize the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Forms a tight heterodimer with protein S6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314951.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRY8"/>
    <dbReference type="SMR" id="Q3SRY8"/>
    <dbReference type="STRING" id="323098.Nwi_1692"/>
    <dbReference type="EnsemblBacteria" id="ABA04953">
        <property type="protein sequence ID" value="ABA04953"/>
        <property type="gene ID" value="Nwi_1692"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1692"/>
    <dbReference type="eggNOG" id="COG0238">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148710_2_3_5"/>
    <dbReference type="OMA" id="QKKYCRF"/>
    <dbReference type="OrthoDB" id="1940575at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00270">
        <property type="entry name" value="Ribosomal_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001648">
        <property type="entry name" value="Ribosomal_S18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018275">
        <property type="entry name" value="Ribosomal_S18_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036870">
        <property type="entry name" value="Ribosomal_S18_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13479">
        <property type="entry name" value="PTHR13479"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01084">
        <property type="entry name" value="Ribosomal_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00974">
        <property type="entry name" value="RIBOSOMALS18"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46911">
        <property type="entry name" value="SSF46911"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00165">
        <property type="entry name" value="S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00057">
        <property type="entry name" value="RIBOSOMAL_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000003548" description="30S ribosomal protein S18">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00270"/>
        </source>
    </evidence>
    <sequence length="79" mass="9067" checksum="797B946BD2DE2055" modified="2005-10-11" version="1">MADAGARRPFFRRRKTCPFTGANAPKIDYKDSKLLMRYVSERGKIVPSRITAVSAKKQRELARAVKRARFLGLLPYVIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS05</accession>
    <name>Q3SS05_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DNA polymerase III, chi subunit</fullName>
            <ecNumber evidence="1">2.7.7.7</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1675</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.7" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314935.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1675"/>
    <dbReference type="EnsemblBacteria" id="ABA04936">
        <property type="protein sequence ID" value="ABA04936"/>
        <property type="gene ID" value="Nwi_1675"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1675"/>
    <dbReference type="eggNOG" id="COG2927">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131584_4_0_5"/>
    <dbReference type="OMA" id="LDAHLWT"/>
    <dbReference type="OrthoDB" id="1575254at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007459">
        <property type="entry name" value="DNA_pol3_chi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036768">
        <property type="entry name" value="PolIII_chi_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38767">
        <property type="entry name" value="PTHR38767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04364">
        <property type="entry name" value="DNA_pol3_chi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102400">
        <property type="entry name" value="SSF102400"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="150" mass="17315" checksum="D2EB2752258C479D" modified="2005-10-11" version="1">MTEVLFYHLQNMSLEKVLPPLLEKTFERGWRAVVHTTSEERADALDAHLWSYRDDSFLPHATWRIEDAGEQPIVLTVDGRNPNRATVRFLIDNAPLPENLDTYERLVLIFDGDDGAALDAARAIWTECKSRGCEATYWQPDETGRWQRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS07</accession>
    <name>Q3SS07_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ABC transporter, ATPase subunit</fullName>
            <ecNumber evidence="8">3.6.3.30</ecNumber>
            <ecNumber evidence="8">3.6.3.41</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_1673</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in beta-(1--&gt;2)glucan export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.30" evidence="8"/>
    <dbReference type="EC" id="3.6.3.41" evidence="8"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314933.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1673"/>
    <dbReference type="EnsemblBacteria" id="ABA04934">
        <property type="protein sequence ID" value="ABA04934"/>
        <property type="gene ID" value="Nwi_1673"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1673"/>
    <dbReference type="eggNOG" id="COG0488">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_36_0_5"/>
    <dbReference type="OMA" id="CTHIADI"/>
    <dbReference type="OrthoDB" id="1112046at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037118">
        <property type="entry name" value="Val-tRNA_synth_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="7">
        <location>
            <begin position="2"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="7">
        <location>
            <begin position="311"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="525"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="529"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="344"/>
            <end position="351"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA04934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="625" mass="68918" checksum="327C773408E5A7DC" modified="2005-10-11" version="1">MLSITDISIRIAGRLLIDHSSVQIVPGARVGFVGRNGVGKSTLFHAIRGELPTETGTIAIPPRWRVGSLAQEAPDGPESLIEVVLKADLERHALLREAETARDPERIADIQMRLVDIDAHSAPARAASILSGLGFSTADQSRPCSEFSGGWRMRVALAATLFASPDLLLLDEPTNYLDLEGTLWLEDHLAHYPRTVIIISHDRDLLDTSVDQILHLDRGKLSLYKGTYSAFEEQRATRELLDAKHARRQADERKRLQAFVDRFKAKASKARQAQSRVKMLERMKPVTALVTQDVREISFPVPDKLLSPPIIAVDEAVVGYDPQSPILKRVTLRVDEDDRIALLGANGNGKSTLVKLLAGKLQPFAGRVVRADKLSIGYFAQHQTDELDLDGSPYDHVRRLMQDAPETKIRARVGGIGFSGKAGDTLVRSLSGGEKARLLLGLATFFAPNMIILDEPTNHLDIDSRAALAEAINEYPGAVIMVSHDRYLIEACADQLWVVADHTVTPYDGDLEDYRRAVLSARGLRSNGRDRVSGQRDDGRDKPRRERTERRSPLKQKITAAEAEIERITGIIAKIDVALALPDLFKREPKQAAQLTKARASAADALRRAEEAWLEASSSYDNATG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS18</accession>
    <name>Q3SS18_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Iron-regulated ABC transporter permease protein SufD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1662</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0051 (ycf24) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314922.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1662"/>
    <dbReference type="EnsemblBacteria" id="ABA04923">
        <property type="protein sequence ID" value="ABA04923"/>
        <property type="gene ID" value="Nwi_1662"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1662"/>
    <dbReference type="eggNOG" id="COG0719">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026231_5_2_5"/>
    <dbReference type="OMA" id="PNCESHQ"/>
    <dbReference type="OrthoDB" id="422804at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000825">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037284">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011542">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045595">
        <property type="entry name" value="SufBD_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43575">
        <property type="entry name" value="PTHR43575"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01458">
        <property type="entry name" value="SUFBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19295">
        <property type="entry name" value="SufBD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101960">
        <property type="entry name" value="SSF101960"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01981">
        <property type="entry name" value="sufD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SufBD_N" evidence="2">
        <location>
            <begin position="28"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="440" mass="46868" checksum="2AD3D6191E5ECA28" modified="2005-10-11" version="1">MDVALVKPTTERATRELFAAARDRLPGSGPVSEARQAAFDDFARRGLPYRRIEEWKYTDLRTLLREIAPLAAAPDQAALARAGEAVTAYGAGDAYKLVLVDGVFAPKLSDQTPENGVEVRALREVLENNEVAARADLLTTTAASDTMISLNTAMATDGIVIDVAENAKLTRPLHIVHIATQSSTAAFTRSSLRLANGAQATLVESFVAAEGAAAYQAHDSVVLWVGDDAVLQHVRLVEDARDAANISTAIVTIGAKATFNSLNLTSGAAVSRYQGFIKFSGEGSHADVNGVNLLNGRQHGDTTLVLDHAVPHCSSNEVFRAVLDDQAHSVFQGKIIVRPDAQKTDGRMMTRALLLSDSAEADNKPELEIFADDVSCGHGATIGALDESLLFYLRARGLSEKEAQALLIQAFVGEAIESVADDGLRDVVIGAAERWLAARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS38</accession>
    <name>Q3SS38_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">tRNA synthetase, class II (D, K and N)</fullName>
            <ecNumber evidence="3">6.1.1.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1642</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.1.1.6" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314902.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1642"/>
    <dbReference type="EnsemblBacteria" id="ABA04903">
        <property type="protein sequence ID" value="ABA04903"/>
        <property type="gene ID" value="Nwi_1642"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1642"/>
    <dbReference type="eggNOG" id="COG2269">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008255_1_0_5"/>
    <dbReference type="OMA" id="EWYRPGF"/>
    <dbReference type="OrthoDB" id="63621at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004824">
        <property type="term" value="F:lysine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006430">
        <property type="term" value="P:lysyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004525">
        <property type="entry name" value="EpmA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018149">
        <property type="entry name" value="Lys-tRNA-synth_II_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00982">
        <property type="entry name" value="TRNASYNTHLYS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00462">
        <property type="entry name" value="genX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="2">
        <location>
            <begin position="23"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="347" mass="38579" checksum="02A9603186ABD4D5" modified="2005-10-11" version="1">MERISSWWDQSRHADRMPFLKARSAVTRALRAWFDAQGFVEVETGVLQVSPGNETHLHAPRTTLTTAAGDHLTRYLRTSPEFACKKLLAAGEQKIVEFARVFRDRERGVLHLPEFTMLEWYRAGGSYEAVMADCITVIANAARATGIPRFSFRGRQADPLAEPERLTVTAAFERFAGIDLLNTIVGREGDRTALAEAARERVRITDDDTWSDIFSKVLVEHVEPHLGQGRLTVLSDYPAPEAALARISPSDSRVAERFEIYACGVELANGFGELTDAAEQRIRFAQAMDDKQIRYGERYPLDEDFLAAVARMPEAGGVALGLDRLVMLASGASRIDQVVWTPPAEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS52</accession>
    <name>Q3SS52_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phage portal protein, HK97</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1628</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314888.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1628"/>
    <dbReference type="EnsemblBacteria" id="ABA04889">
        <property type="protein sequence ID" value="ABA04889"/>
        <property type="gene ID" value="Nwi_1628"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1628"/>
    <dbReference type="eggNOG" id="COG4695">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033789_5_0_5"/>
    <dbReference type="OMA" id="WPFRRTT"/>
    <dbReference type="OrthoDB" id="389520at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006944">
        <property type="entry name" value="Phage/GTA_portal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006427">
        <property type="entry name" value="Portal_HK97"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04860">
        <property type="entry name" value="Phage_portal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01537">
        <property type="entry name" value="portal_HK97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="371"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="374"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="392" mass="42251" checksum="CEC7D53725039BC4" modified="2005-10-11" version="1">MKLWPFRRTTIETKSLADPDEAIFAILSGGSTYGPVHPLSEPAVSAAVTTISNAAATLDLHLVKRDDTSADVKHPALDLLRGHVNEWTSGSDLIRDLITQALTADAGGIAWVNKSGDGRPLEIIRYTAGRITVEYAGDGSGRPSYTVNGRSVPASDVVHIRGPFSKCPVSLAYPTISAAVAMSRYVEFLFKRMARPGGVVEVPTGAGEKAVQNMIAGWNAAYGGADNAGGTAFLFDGATFKQIALASTDAQFVENRRWQLEDIARAFNISSVMLGDLTKSSYANAWQKYREFLSVTLMPWLKALESAFDRALLTDDERALYAFKFDIDDLTRVDLEKRATAISSLVASRVLNPNEARTWLDTGLAPYAGGNEFANPNTGASQPGSQEQPVND</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS53</accession>
    <name>Q3SS53_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1484</name>
        <name evidence="2" type="ordered locus">Nwi_1627</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="2">Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314752.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1484"/>
    <dbReference type="EnsemblBacteria" id="ABA04745">
        <property type="protein sequence ID" value="ABA04745"/>
        <property type="gene ID" value="Nwi_1484"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04888">
        <property type="protein sequence ID" value="ABA04888"/>
        <property type="gene ID" value="Nwi_1627"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1484"/>
    <dbReference type="KEGG" id="nwi:Nwi_1627"/>
    <dbReference type="eggNOG" id="ENOG50328EB">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1955677_0_0_5"/>
    <dbReference type="OMA" id="VPFSHAN"/>
    <dbReference type="OrthoDB" id="1898591at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="129" mass="14096" checksum="86CCA0265C6D04C9" modified="2005-10-11" version="1">MTDLADFDDLIAYQDRGAECVITDPVNGTPTDIVLIVAGPDSDTQRRARLATTDALMSYAGRPPADEQERLAIEQLAKCVVGWGVKQGGVDVPFSFSAAVRLLTKFRFVREQVDAFAASREPYSRKVTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS95</accession>
    <name>Q3SS95_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chitooligosaccharide deacetylase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Nodulation protein B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_1585</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Is involved in generating a small heat-stable compound (Nod), an acylated oligomer of N-acetylglucosamine, that stimulates mitosis in various plant protoplasts.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the polysaccharide deacetylase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314852.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1585"/>
    <dbReference type="EnsemblBacteria" id="ABA04846">
        <property type="protein sequence ID" value="ABA04846"/>
        <property type="gene ID" value="Nwi_1585"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1585"/>
    <dbReference type="eggNOG" id="COG0726">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021264_8_1_5"/>
    <dbReference type="OMA" id="KATFCEI"/>
    <dbReference type="OrthoDB" id="1701876at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0536">Nodulation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229242" description="Chitooligosaccharide deacetylase" evidence="7">
        <location>
            <begin position="24"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="NodB homology" evidence="8">
        <location>
            <begin position="85"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA04846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="308" mass="33511" checksum="C5F7E2E2AE2C8386" modified="2005-10-11" version="1">MRMTAVLISAGVISLCAATVAWSQTPAPKGAQNAAPVPVRASGPQVACNGSPDALGVARVVQIDTTGGPGFGFEHFKQLDFLRDKEVVLTFDDGPWPGKTPAVLKALADQCTTGIFFSIGKHATYHPEILRNVAAAGHTIGGHTWSHANLNNKKLSEDQRKEEIEKGFSAVKWALEAEPAPFFRFPALQHPPEIVTYLGERNVAIFSCDVDSFDFKTRNAEQVVTNIFKKLDKVGKGIILMHDFQKHTAEALPEILRRLKAGGYKVAQMKAKAPIQTLAQYDEALKKDTKLPTVSTRPIDSVVQTISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS97</accession>
    <name>Q3SS97_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">CreA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1583</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314850.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04844">
        <property type="protein sequence ID" value="ABA04844"/>
        <property type="gene ID" value="Nwi_1583"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1583"/>
    <dbReference type="eggNOG" id="COG3045">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109726_1_0_5"/>
    <dbReference type="OMA" id="MPWGASD"/>
    <dbReference type="OrthoDB" id="1618795at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010292">
        <property type="entry name" value="Uncharacterised_CreA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37952">
        <property type="entry name" value="PTHR37952"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05981">
        <property type="entry name" value="CreA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003174">
        <property type="entry name" value="CreA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="186" mass="20862" checksum="83421B451B13A343" modified="2005-10-11" version="1">MTFRARIHDERRRTAQTLLAFIVLTIAMLAGTYAHAADEPDLIFRRSTVFKLLTPNDKLATYGVDDPGIEGVACHFTVPEKGGIKGWLGVAEEVSDISLACRQTGPIRFKEKLKQGDDIFRQRRSLFFKKMQIVRGCDVKRNVLVYMVYSDRVIEGSPQNSTSSIPIMPWGASDTAMQKCADFISD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSD8</accession>
    <name>Q3SSD8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phage major capsid protein, HK97</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1542</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314809.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1542"/>
    <dbReference type="EnsemblBacteria" id="ABA04803">
        <property type="protein sequence ID" value="ABA04803"/>
        <property type="gene ID" value="Nwi_1542"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1542"/>
    <dbReference type="eggNOG" id="COG4653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041417_0_0_5"/>
    <dbReference type="OMA" id="PYRNKAV"/>
    <dbReference type="OrthoDB" id="755947at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024455">
        <property type="entry name" value="Phage_capsid"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05065">
        <property type="entry name" value="Phage_capsid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01554">
        <property type="entry name" value="major_cap_HK97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="26"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="419" mass="45705" checksum="A6AC40A36B4D35A2" modified="2005-10-11" version="1">MTIAMKSRARGLVAARADAGSATAILNELRQTFETFKAEREKEIADLKKGLGDVVQSEKVDRINAEITKLQEQLDQVNSSIAALKVGGAGDDKPLAAERREHARAFNQFFRNGAENGLRDLEVKAALRTDSDPDGGFVVPDQMEAAIDRVLGTVSAMRAISRVMSISSGTYKKLVNQGGAVGGWVGERQARPATATPTLVELAFQAMELYANPAATQTLLDDSRVNIEQWLADEVSITFAEMEGASFIIGDGVGKPRGLLSYDTVADTSYAWGKLGYVVSGVAAAMTDSSHNGADALTDLVYSIKQGYRQNARFLMNRKTQAAIRKFKSKTEELYLWQPSIQAGQPATILGYPVTDDDNMPDATAGGNFPIAFGDFQRGYLIVDRMGVRVLRDPFTNKPYVHFYTTCPSSDNLRLIRHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSE3</accession>
    <name>Q3SSE3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1537</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1537"/>
    <dbReference type="EnsemblBacteria" id="ABA04798">
        <property type="protein sequence ID" value="ABA04798"/>
        <property type="gene ID" value="Nwi_1537"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1537"/>
    <dbReference type="eggNOG" id="ENOG5030WUE">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099757_0_0_5"/>
    <dbReference type="OMA" id="HIFGRII"/>
    <dbReference type="OrthoDB" id="1541083at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229245" evidence="1">
        <location>
            <begin position="22"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="178" mass="19608" checksum="D2D05E34F9629DC9" modified="2005-10-11" version="1">MRMAVILAALCAVVFVSQADARPKQTTSIMSRSSFAHHPKQLISRRNRRKTGHLITAHRDTTRPLPPGLHRIMATGAGRIVGGRPRGCPRRYCGCGASLHIFGRIIPRLNLAANWLRFPRAAPAPGMVAARRGHVFVLKRHIRGKVWLVWDANSGRGKTRVHPRSIAGFTIVDPRAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSH2</accession>
    <name>Q3SSH2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hemimethylated DNA-binding region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1508</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1508"/>
    <dbReference type="EnsemblBacteria" id="ABA04769">
        <property type="protein sequence ID" value="ABA04769"/>
        <property type="gene ID" value="Nwi_1508"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1508"/>
    <dbReference type="eggNOG" id="COG3785">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123865_0_1_5"/>
    <dbReference type="OMA" id="PYYHLLA"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011722">
        <property type="entry name" value="Hemimethylated_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036623">
        <property type="entry name" value="Hemimethylated_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08755">
        <property type="entry name" value="YccV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00992">
        <property type="entry name" value="YccV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141255">
        <property type="entry name" value="SSF141255"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02097">
        <property type="entry name" value="yccV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YccV-like" evidence="1">
        <location>
            <begin position="10"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="114" mass="13326" checksum="CFA6EE42749D627E" modified="2005-10-11" version="1">MRSGMTKARTARFQIGQIVRHRIYSFRGVIFDIDPVFNNTEEWWLSIPENVRPHKDQPFYHMLAENADSEYVAYVSEQNLLPDDSGEPIRHSQVTEIFIQDESGGYRPRNPSLN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSN1</accession>
    <name>HFQ_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-binding protein Hfq</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hfq</name>
        <name type="ordered locus">Nwi_1449</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">RNA chaperone that binds small regulatory RNA (sRNAs) and mRNAs to facilitate mRNA translational regulation in response to envelope stress, environmental stress and changes in metabolite concentrations. Also binds with high specificity to tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Hfq family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009797508.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSN1"/>
    <dbReference type="SMR" id="Q3SSN1"/>
    <dbReference type="STRING" id="323098.Nwi_1449"/>
    <dbReference type="EnsemblBacteria" id="ABA04710">
        <property type="protein sequence ID" value="ABA04710"/>
        <property type="gene ID" value="Nwi_1449"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1449"/>
    <dbReference type="eggNOG" id="COG1923">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113688_0_0_5"/>
    <dbReference type="OMA" id="QQMVYKH"/>
    <dbReference type="OrthoDB" id="1989988at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01716">
        <property type="entry name" value="Hfq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00436">
        <property type="entry name" value="Hfq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005001">
        <property type="entry name" value="Hfq"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34772">
        <property type="entry name" value="PTHR34772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17209">
        <property type="entry name" value="Hfq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="SSF50182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02383">
        <property type="entry name" value="Hfq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0346">Stress response</keyword>
    <feature type="chain" id="PRO_1000080673" description="RNA-binding protein Hfq">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00436"/>
        </source>
    </evidence>
    <sequence length="82" mass="9235" checksum="AAC887AE764CF6B2" modified="2005-10-11" version="1">MAIDRAQNLQDTFLNHVRKTKTPLTIFLVNGVKLQGIVTWFDNFCLLLRRDGHSQLVYKHAISTIMPGAPIQLFEGGEDAPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSP3</accession>
    <name>Q3SSP3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_1437</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314706.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1437"/>
    <dbReference type="EnsemblBacteria" id="ABA04698">
        <property type="protein sequence ID" value="ABA04698"/>
        <property type="gene ID" value="Nwi_1437"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1437"/>
    <dbReference type="eggNOG" id="COG2807">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038046_4_2_5"/>
    <dbReference type="OMA" id="GGYTSMV"/>
    <dbReference type="OrthoDB" id="806182at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="131"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="274"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="336"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="360"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="11"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="388" mass="40203" checksum="30E8F6AC0310ABBC" modified="2005-10-11" version="1">MSVARSSSLRPLGLLWLGGVGLRLTILAVPPVLALIISDLALSGTEVGILNAIPVSLFALVAVPGSLLISRVGAVRALVVGLLIAAVGSALRGFAIGAMTLFAATALMAAGVALMQPALPPLVRQWVPRRIGFATAVYTNGLLCGEIFPVVLVVVTVPLLGGGWRGSLELWSIAAAAIALIIFVARPGGDQVSAQHSRWMPDWRDPLLWKIGLVASANNQLYFCTNAFLPGLLLQNGQTELIGPALSALNLGQLPGSIILILMASRLERRRWPLVLCGALGLLAVLGTATSTSFWAIVGSAALVGFTCAVGLTLVLTLPALLVAPDDVPHNSAGMFTIGYGIAMLISIVSGMVWDITGNAVVAFVPIGLAVVPMVVLPLGIDFSTRRS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSW2</accession>
    <name>RS19_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsS</name>
        <name type="ordered locus">Nwi_1368</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009797134.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSW2"/>
    <dbReference type="SMR" id="Q3SSW2"/>
    <dbReference type="STRING" id="323098.Nwi_1368"/>
    <dbReference type="EnsemblBacteria" id="ABA04629">
        <property type="protein sequence ID" value="ABA04629"/>
        <property type="gene ID" value="Nwi_1368"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1368"/>
    <dbReference type="eggNOG" id="COG0185">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144911_0_1_5"/>
    <dbReference type="OMA" id="FPEMVGH"/>
    <dbReference type="OrthoDB" id="1893906at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.860.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00531">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020934">
        <property type="entry name" value="Ribosomal_S15/S19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002222">
        <property type="entry name" value="Ribosomal_S19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005732">
        <property type="entry name" value="Ribosomal_S19_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023575">
        <property type="entry name" value="Ribosomal_S19_S15_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880">
        <property type="entry name" value="PTHR11880"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00203">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002144">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00975">
        <property type="entry name" value="RIBOSOMALS19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54570">
        <property type="entry name" value="SSF54570"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01050">
        <property type="entry name" value="rpsS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00323">
        <property type="entry name" value="RIBOSOMAL_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000265390" description="30S ribosomal protein S19">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00531"/>
        </source>
    </evidence>
    <sequence length="92" mass="10262" checksum="81AB0F2CDC16555A" modified="2005-10-11" version="1">MVRSVWKGPFVEGSLLKKADAARASGRHDVIKIWSRRSTILPQFVGLVFGVYNGQKHVPVSVNEEMVGHKFGEFSPTRTFHGHSGDKKAKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSZ9</accession>
    <name>Q3SSZ9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SLT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1331</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the virb1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314610.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1331"/>
    <dbReference type="CAZy" id="GH23">
        <property type="family name" value="Glycoside Hydrolase Family 23"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04592">
        <property type="protein sequence ID" value="ABA04592"/>
        <property type="gene ID" value="Nwi_1331"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1331"/>
    <dbReference type="eggNOG" id="COG0741">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062432_2_0_5"/>
    <dbReference type="OMA" id="LYMAHFM"/>
    <dbReference type="OrthoDB" id="1578495at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008258">
        <property type="entry name" value="Transglycosylase_SLT_dom_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01464">
        <property type="entry name" value="SLT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SLT" evidence="3">
        <location>
            <begin position="23"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="286"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="314" mass="32593" checksum="72581FA7F3F6F8AE" modified="2005-10-11" version="1">MNVDSANAVSGLSGVFGGITTAIRRAAEATGASFEYLLATAKMESNFNPHAAAGTSSAKGLYQFIEQTWLGTVKEAGPALGFNGYADAIARSPSGTYSVADPAARAAILKLRDDPAIASAMAGVLTKSNSFKLTGLIGRRPTDSELYMAHFMGVSGAAKLIANAESNPQASGALLFPNAAAANRPIFYDHSGRARSVSEVYADLDRRYAVAANSPATRMAAASGTQFSTPSDRAAYLSRLPDVGGVAPVAASSSVAPSQPMFRSLFQVGERAQPVSPVVQELWGVPSTTAQNPSTSDPRPPFDLFSDRSGAYSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST05</accession>
    <name>Q3ST05_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314604.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1325"/>
    <dbReference type="EnsemblBacteria" id="ABA04586">
        <property type="protein sequence ID" value="ABA04586"/>
        <property type="gene ID" value="Nwi_1325"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1325"/>
    <dbReference type="eggNOG" id="ENOG50308U9">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058985_0_0_5"/>
    <dbReference type="OMA" id="ESIGAFP"/>
    <dbReference type="OrthoDB" id="1070800at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="349" mass="36898" checksum="DC99E82BC336259E" modified="2005-10-11" version="1">MIFGGNHRYRSGRRSWPLLVSVVTLLATYPHISSAQLFSERPPPVPPESVPEAPPPGAAISLAPSADPASMPNLPAPLTQAPVAAVPLTSSPRVASPAQAVLSLTARYGKDLPAINSGLIWRVYSDRPDETGAFKLVREDHEATPSIALPPGDYVVHVSLGLVSAARPVTLRSDADRESFVLPAGGLKIEGRVGASMIPPNQISFSIYKGSQFEGTERAPLLPNVAGGDVMLLPEGTYYIVSNYGDANSVVRSDIRVQAGKLTDVTISHRAAVITLKLVSDKGGEALANTAWSVLTPGGDVIKESIGAFPRVVLAEGEYRVIAKNEGKVFERGFEVSNGVDGEIEVLAR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST22</accession>
    <name>PURL_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribosylformylglycinamidine synthase subunit PurL</fullName>
            <shortName evidence="1">FGAM synthase</shortName>
            <ecNumber evidence="1">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Formylglycinamide ribonucleotide amidotransferase subunit II</fullName>
            <shortName evidence="1">FGAR amidotransferase II</shortName>
            <shortName evidence="1">FGAR-AT II</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glutamine amidotransferase PurL</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphoribosylformylglycinamidine synthase subunit II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purL</name>
        <name type="ordered locus">Nwi_1308</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ADP + H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FGAMS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314589.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ST22"/>
    <dbReference type="SMR" id="Q3ST22"/>
    <dbReference type="STRING" id="323098.Nwi_1308"/>
    <dbReference type="EnsemblBacteria" id="ABA04569">
        <property type="protein sequence ID" value="ABA04569"/>
        <property type="gene ID" value="Nwi_1308"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1308"/>
    <dbReference type="eggNOG" id="COG0046">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003100_0_1_5"/>
    <dbReference type="OMA" id="FIEPYQG"/>
    <dbReference type="OrthoDB" id="26038at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00420">
        <property type="entry name" value="PurL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010074">
        <property type="entry name" value="PRibForGlyAmidine_synth_PurL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041609">
        <property type="entry name" value="PurL_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43555">
        <property type="entry name" value="PTHR43555"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18072">
        <property type="entry name" value="FGAR-AT_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001587">
        <property type="entry name" value="FGAM_synthase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01736">
        <property type="entry name" value="FGAM_synth_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000236656" description="Phosphoribosylformylglycinamidine synthase subunit PurL">
        <location>
            <begin position="1"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="95"/>
            <end position="98"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="313"/>
            <end position="315"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="494"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="531"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="532"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="534"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00420"/>
        </source>
    </evidence>
    <sequence length="737" mass="78779" checksum="921A14985E641AC2" modified="2005-10-11" version="1">MIVRNEPPITPELVASHGLKPDEYERILKLLGRTPTFTELGIFSAMWNEHCSYKSSRIHLRGLPTKAPWVIQGPGENAGVIDIGDGQAVVFKMESHNHPSYIEPYQGATTGVGGILRDVFTMGARPIACLNALSFGDPSHPKTRHLVSGVVAGVGGYGNSFGVPTVGGQVRFHTRYDGNILVNAMAVGLADADRIFYAAASGVNMPIVYLGSKTGRDGIHGATMASAEFDDDSEEKRPTVQVGDPFAEKLLLEACLEIMAKGCVVAIQDMGAAGLTCSAVEMGAKGDLGVDLDLDSVPTRETGMSAYEMMLSESQERMLMVLKPEKEKEAEAIFRKWGLDFAIVGSTTPGRRFVVRHGGDVMADLPIKELESEAPLYDRPHVPSPQLPVIQARDVAPSLTTADALEKLLAMPELCSKRWVWEQYDHVIGGNTVQRPGGDAAVVRVEDGPKGLALTVDVTPRYCEADPFEGGKQAVAEAWRNITAVGGRPLAITDNLNFGNPERPENMGQFVGCLKGIAAACTALDFPVVSGNVSLYNETNGRGILPTPTIGGVGLLDDFTKSASLAFKAEGHPILLIGDTQGWLGQSVYLRDICRREEGAPPPVDLAAEKRNGDVVRGMIHAGTATAVHDVSDGGLLVALAEMAMADGIGAALDAAPAAIVPYAWWFGEDQARYIVTVHERDLLSVFTKLKAVNVPCIQIGLTGGHEIAIAGERAVDLKVLRQGFESWLPDYMAGKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST42</accession>
    <name>Q3ST42_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1288</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04549">
        <property type="protein sequence ID" value="ABA04549"/>
        <property type="gene ID" value="Nwi_1288"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1288"/>
    <dbReference type="HOGENOM" id="CLU_2554850_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="82" mass="8972" checksum="30FFD64461D9102A" modified="2005-10-11" version="1">MRRDFSVTLQGELSTILDWIERTGKPGYKPNPDARSTRLSVSVKARACPGHPRLVCGYAAKTWMPATSAGTTVKIICARNPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST55</accession>
    <name>Q3ST55_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1275</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04536">
        <property type="protein sequence ID" value="ABA04536"/>
        <property type="gene ID" value="Nwi_1275"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1275"/>
    <dbReference type="eggNOG" id="ENOG502ZYZ5">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1979199_0_0_5"/>
    <dbReference type="OMA" id="APIQKYG"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="126" mass="13648" checksum="EFEAB8877A9D4231" modified="2005-10-11" version="1">MMMRSSHGCRAASRNLRALSSGEPLETKRIARLCCPLWGGVLMKILCASMLMAAVVSGPAFAQAPQFNMMADGPGKTPEQIERQKALEKAYKESLRKIPDAAVVDDPWGGVRNASQSKTSSKSRQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST88</accession>
    <name>Q3ST88_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Putrescine-binding periplasmic protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_1241</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for the activity of the bacterial periplasmic transport system of putrescine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial solute-binding protein 1 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the bacterial solute-binding protein PotD/PotF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1241"/>
    <dbReference type="EnsemblBacteria" id="ABA04503">
        <property type="protein sequence ID" value="ABA04503"/>
        <property type="gene ID" value="Nwi_1241"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1241"/>
    <dbReference type="eggNOG" id="COG0687">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026974_1_4_5"/>
    <dbReference type="OMA" id="SNWTEYM"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019808">
        <property type="term" value="F:polyamine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015846">
        <property type="term" value="P:polyamine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001188">
        <property type="entry name" value="Sperm_putr-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13416">
        <property type="entry name" value="SBP_bac_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019574">
        <property type="entry name" value="Periplasmic_polyamine_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00909">
        <property type="entry name" value="SPERMDNBNDNG"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0574">Periplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229179" description="Putrescine-binding periplasmic protein" evidence="8">
        <location>
            <begin position="30"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>spermidine</name>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR019574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR019574-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA04503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="371" mass="41374" checksum="5608E5EDA1CB67C9" modified="2005-10-11" version="1">MIPNQCVWFWAPVALMLGSGAVLSPRADAAERVVNFFNWSNYIAPGVLEAFTEETGIKVVYDTFDANETLETRLLAGKSGYDIVVPTAYFLQRQIGANIFRKLDKSKLPNLANAWPMVTERLATYDPGNQYAVNYMWGTTGIGYNVKAVRRILGPDSRIDSWDVIFDPDKLAKFKDCGVQMLDSADDILPAALNFLGLDPNSTEKSDLEQAAALVGKIRPYVRKFHSSEYLNSLATGEICLVVGWSGDIKQAQSRAAESHNGVEIGYAIPKEGAQMFFDNLAVLLDARHVDEAYELINYLYRPDVAAKNSDFLSYASGNLASQKLIDPQAFNDKTIYPDEAVMKRLFIITARDLATQRSINRLWTRIKTGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STG5</accession>
    <name>Q3STG5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1164</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1164"/>
    <dbReference type="EnsemblBacteria" id="ABA04426">
        <property type="protein sequence ID" value="ABA04426"/>
        <property type="gene ID" value="Nwi_1164"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1164"/>
    <dbReference type="HOGENOM" id="CLU_2233676_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="105" mass="11462" checksum="EC39B95F1E5444A4" modified="2005-10-11" version="1">MTSHESAFEAAQRACWMRPDAYRWIRPDAVRFLAPGTDPREVYPALQRKFNPDQPRVPAGNPDAGGGRMAAVAVSSRFNIGRASDPLTCWKSAAWVATPSKGMSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STJ0</accession>
    <name>Q3STJ0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1139</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314432.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04401">
        <property type="protein sequence ID" value="ABA04401"/>
        <property type="gene ID" value="Nwi_1139"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1139"/>
    <dbReference type="eggNOG" id="COG5330">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_857041_0_0_5"/>
    <dbReference type="OMA" id="TRAKYRA"/>
    <dbReference type="OrthoDB" id="1087965at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019285">
        <property type="entry name" value="DUF2336"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10098">
        <property type="entry name" value="DUF2336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="290"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="321" mass="34998" checksum="473C35259821CBAB" modified="2005-10-11" version="1">MTAPPMFPGFDGLLTLSRRDGVDIRPTLLRVLTDLYVQTSTHTVEEERHFVELTSRLIDHVDDATRAVVRARLSIYPRTPAAIMNKLRLGAFAADPRPDSPPPQATPLKAPPAETRTRPVAILSMEPRDAAEISEMFFGANSKDRALILHNLATAPLRPSPHIHPSRAARAIEVLHMAAFASDVGNFATEISATLMLPAAIAERVVKDPGGEPLACAAKAIGIPSQTFQQILLFLDPAIGTSVTEVYRLARLYDVLSERAARIMLAAWRGATMTEARAKYRPALYHEERHRARAVPANSRPEGVQSGRGSLRWSGRDGSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STM6</accession>
    <name>Q3STM6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Flagellar FlbT</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1103</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314396.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1103"/>
    <dbReference type="EnsemblBacteria" id="ABA04365">
        <property type="protein sequence ID" value="ABA04365"/>
        <property type="gene ID" value="Nwi_1103"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1103"/>
    <dbReference type="eggNOG" id="COG5443">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130913_0_0_5"/>
    <dbReference type="OMA" id="INGWTES"/>
    <dbReference type="OrthoDB" id="1771106at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048027">
        <property type="term" value="F:mRNA 5'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902209">
        <property type="term" value="P:negative regulation of bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009967">
        <property type="entry name" value="Flagellum_FlbT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07378">
        <property type="entry name" value="FlbT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009533">
        <property type="entry name" value="FlbT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0966">Cell projection</keyword>
    <keyword evidence="1" id="KW-0969">Cilium</keyword>
    <keyword evidence="1" id="KW-0282">Flagellum</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="142" mass="15965" checksum="E7D053FD8280ECAB" modified="2005-10-11" version="1">MALKVELKPHERIIIGACVVTNTDQRARLLIEGDKIPILREKDILTPETADTPAKLIYLAVQLMYLSPEPQINHGTYFSLLRDVITTMPGAWPIIEGINNDILNGDLYKALKEVKKLIAYEKKLADMSDTPPNRAGDRRRSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STN0</accession>
    <name>Q3STN0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Flagellar hook protein FlgE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_1099</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Bacterial flagellum basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the flagella basal body rod proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314392.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1099"/>
    <dbReference type="EnsemblBacteria" id="ABA04361">
        <property type="protein sequence ID" value="ABA04361"/>
        <property type="gene ID" value="Nwi_1099"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1099"/>
    <dbReference type="eggNOG" id="COG1749">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013687_2_3_5"/>
    <dbReference type="OMA" id="FVFTYGD"/>
    <dbReference type="OrthoDB" id="704242at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011491">
        <property type="entry name" value="Falgellar_hook_FlgE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001444">
        <property type="entry name" value="Flag_bb_rod_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020013">
        <property type="entry name" value="Flagellar_FlgE/F/G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010930">
        <property type="entry name" value="Flg_bb/hook_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037925">
        <property type="entry name" value="FlgE/F/G-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07559">
        <property type="entry name" value="FlaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00460">
        <property type="entry name" value="Flg_bb_rod"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06429">
        <property type="entry name" value="Flg_bbr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117143">
        <property type="entry name" value="SSF117143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03506">
        <property type="entry name" value="FlgEFG_subfam"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0969">Cilium</keyword>
    <keyword evidence="9" id="KW-0282">Flagellum</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flg_bb_rod" evidence="6">
        <location>
            <begin position="7"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="FlaE" evidence="8">
        <location>
            <begin position="381"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="Flg_bbr_C" evidence="7">
        <location>
            <begin position="540"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA04361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="613" mass="61633" checksum="ED4D7FC778B0224D" modified="2005-10-11" version="1">MGIFDALNTSVGGLQAQSFALQNISGNIANAQTTGYKGITTAFSDLIPDSTAPNRQAAGGVTAYATATISSQGTVSASTVGTYMAITGDGFFNVQAPTGRMDNQPQFSGVTYYTRRGDFQVDAGGHLVNSAGYYLMGVPVDPKTGNPLGNVPQVLKFQNNFVPAQATTTVQYAANLPSVPRTLSSSTAPAGTITAAGGLNPSDFTTNFNPLVVGTPAPPYTDNITTGSAATNQQTPSGPITSATLLSGVAPSDSLPGGFAVGDTITVNGTTITFTDAGTALPPGVQDATHVRIDDTVGDLLGRIGAITGAAPTIDAGTGAITLHTGTAQDLTVTSASAGWTAMGFGAMINIARGGGGTPGAGVVIANDQTIFQQQSISGGAVTAYDATGTAVNLQLRWAKTDSASLGAGHSDTWNLFYQADPNATGTQAAWINVGTNFTFGINGQLSSPVGSSITIPGVSVGSQSLGNLTLNIGSGGLTQYASSGGNATINAINQNGYAAGQLQSVAVNNDGIVVGTFSNGQNLSLAQVSLSHFNGTNYLKALDGGAYAVTNESGSAIAGASGRISGSSLEGSNADIADEFTKLIVTQQAYSANTKVITTANSMVQDLLNVLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STP1</accession>
    <name>Q3STP1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1088</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adrenodoxin/putidaredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314384.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1088"/>
    <dbReference type="EnsemblBacteria" id="ABA04350">
        <property type="protein sequence ID" value="ABA04350"/>
        <property type="gene ID" value="Nwi_1088"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1088"/>
    <dbReference type="eggNOG" id="COG0633">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082632_5_1_5"/>
    <dbReference type="OMA" id="SACGGVC"/>
    <dbReference type="OrthoDB" id="1837979at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140647">
        <property type="term" value="P:P450-containing electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001055">
        <property type="entry name" value="Adrenodoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018298">
        <property type="entry name" value="Adrenodoxin_Fe-S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426">
        <property type="entry name" value="PTHR23426"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00355">
        <property type="entry name" value="ADRENODOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00814">
        <property type="entry name" value="ADX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="3">
        <location>
            <begin position="2"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="106" mass="11505" checksum="091F543A324679EA" modified="2005-10-11" version="1">MTKINFVDHSGETRTVDVENGATVMEAAIRNAVPGIEAECGGACACATCHVYIDDAWTEKVGKPTPMEEDMLDFGYDVRPNSRLSCQIKVSDELEGLVVTVPERQA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STS7</accession>
    <name>MURC_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UDP-N-acetylmuramate--L-alanine ligase</fullName>
            <ecNumber evidence="1">6.3.2.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">UDP-N-acetylmuramoyl-L-alanine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">murC</name>
        <name type="ordered locus">Nwi_1052</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cell wall formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-alanine + UDP-N-acetyl-alpha-D-muramate = ADP + H(+) + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine</text>
            <dbReference type="Rhea" id="RHEA:23372"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:70757"/>
            <dbReference type="ChEBI" id="CHEBI:83898"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MurCDEF family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.8" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314348.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3STS7"/>
    <dbReference type="SMR" id="Q3STS7"/>
    <dbReference type="STRING" id="323098.Nwi_1052"/>
    <dbReference type="EnsemblBacteria" id="ABA04314">
        <property type="protein sequence ID" value="ABA04314"/>
        <property type="gene ID" value="Nwi_1052"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1052"/>
    <dbReference type="eggNOG" id="COG0773">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028104_2_2_5"/>
    <dbReference type="OMA" id="DITYQLR"/>
    <dbReference type="OrthoDB" id="307881at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008763">
        <property type="term" value="F:UDP-N-acetylmuramate-L-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00046">
        <property type="entry name" value="MurC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000713">
        <property type="entry name" value="Mur_ligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005758">
        <property type="entry name" value="UDP-N-AcMur_Ala_ligase_MurC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01225">
        <property type="entry name" value="Mur_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01082">
        <property type="entry name" value="murC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0133">Cell shape</keyword>
    <keyword id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000242567" description="UDP-N-acetylmuramate--L-alanine ligase">
        <location>
            <begin position="1"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="114"/>
            <end position="120"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00046"/>
        </source>
    </evidence>
    <sequence length="467" mass="49979" checksum="6CE3FC5F6E01E980" modified="2005-10-11" version="1">MRLPREIGPIHFVGIGGIGMSGIAEVLCNLGYTVQGSDASEGANVVRLREKGVKVTVGHKAGNVNGADVLVVSTAIKRDNPELMAARAQRIPVVRRAEMLAELMRLKSCVAIAGTHGKTTTTSMVATLLDAGDFDPTVINGGIINAYGTNARLGAGEWMVVEADESDGTFLKLPTDVAIVTNVDAEHLDHFKTFDAVQDAFRSFVENVPFYGFAVMCIDHPVVQAMVGRIEDRRIITYGQNPQADVRLVDLAPNGGGSHFKVMFRNRKTDAAHEISDLVLPMPGPHNALNATAAIAVAHELGITDATIRKALAAFGGVKRRFTRTGEWNGVTVIDDYGHHPVEIAAVLKAARESTDGRVIAVVQPHRYTRLQALFEEFCTCFNDADTVVVADVYPAGETPIVGIDRDHFVLGLRAHGHREVVPLPESAALAGIIADLAKQGDYVVCLGAGNITQWAYALPGELKALG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STW5</accession>
    <name>Q3STW5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phasin 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1014</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1014"/>
    <dbReference type="EnsemblBacteria" id="ABA04276">
        <property type="protein sequence ID" value="ABA04276"/>
        <property type="gene ID" value="Nwi_1014"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1014"/>
    <dbReference type="eggNOG" id="COG5490">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119062_0_0_5"/>
    <dbReference type="OMA" id="MEFTKAN"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018968">
        <property type="entry name" value="Phasin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010234">
        <property type="entry name" value="Phasin_subfam-2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09361">
        <property type="entry name" value="Phasin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01985">
        <property type="entry name" value="phasin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phasin_2" evidence="1">
        <location>
            <begin position="43"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="148" mass="16105" checksum="05226B3D430AB337" modified="2005-10-11" version="1">MRRSQHAKDMTMTNPADAFEVPEQVRAFAEKGVLQARDTYARFKDAAQDGNGAIEAVFNAATKGAHDYSTKLLDFGQANTNAGFDFLQALVGAKSLSDATELWSTHTRKQFGTFAAQAKELTELTQKIAVETAEPVRTGASKFFQPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STX8</accession>
    <name>Q3STX8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">N5-carboxyaminoimidazole ribonucleotide synthase</fullName>
            <shortName evidence="3 5">N5-CAIR synthase</shortName>
            <ecNumber evidence="3 5">6.3.4.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3 5">5-(carboxyamino)imidazole ribonucleotide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3 5" type="primary">purK</name>
        <name evidence="7" type="ordered locus">Nwi_1001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).</text>
    </comment>
    <comment type="function">
        <text evidence="5">Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO(3)- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 5">
            <text>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + ATP + hydrogencarbonate = 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + ADP + 2 H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19317"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58730"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 5">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the PurK/PurT family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.18" evidence="3 5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314301.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1001"/>
    <dbReference type="EnsemblBacteria" id="ABA04263">
        <property type="protein sequence ID" value="ABA04263"/>
        <property type="gene ID" value="Nwi_1001"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1001"/>
    <dbReference type="eggNOG" id="COG0026">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011534_0_1_5"/>
    <dbReference type="OMA" id="ITFDHEH"/>
    <dbReference type="OrthoDB" id="1165275at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00942"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034028">
        <property type="term" value="F:5-(carboxyamino)imidazole ribonucleotide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004638">
        <property type="term" value="F:phosphoribosylaminoimidazole carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01928">
        <property type="entry name" value="PurK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003135">
        <property type="entry name" value="ATP-grasp_carboxylate-amine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005875">
        <property type="entry name" value="PurK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040686">
        <property type="entry name" value="PurK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02222">
        <property type="entry name" value="ATP-grasp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17769">
        <property type="entry name" value="PurK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="SSF51246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01161">
        <property type="entry name" value="purK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1 3 5" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="6">
        <location>
            <begin position="115"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="156"/>
            <end position="162"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="186"/>
            <end position="189"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="274"/>
            <end position="275"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="370" mass="39477" checksum="7B25A87788781FDA" modified="2005-10-11" version="1">MTGSRRPTLKPGGTIGILGGGQLGRMLVLAAAHLGLKCQVFSPDPDSPAFDVVQNATCAEYADVEALELFATDVDVITYEFENVPAATAMVLAARRPVLPDHRILETVQDRLLEKNFVTGLGIGTAAYADVASAQALREAIKVIGLPAVIKTRRFGYDGKGQAIIRAGDNVDQVWDDLCTKSAVVEAFVPFEREISVIAARGADGRVECFDVTENEHHDHILKYSRAPAAIPDALAAQARDMAGKIAAALDYIGVLAVEMFVVGGASGPTLLVNEIAPRVHNSGHWTLDGASVSQFEQHIRAVAGWPLGKPVRQGSAVMTNLIGKDVLDYRKWLTVPGAAVHIYGKGPPLPGRKMGHVTEVHVTDVKGRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU21</accession>
    <name>Q3SU21_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0958</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314261.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0958"/>
    <dbReference type="EnsemblBacteria" id="ABA04220">
        <property type="protein sequence ID" value="ABA04220"/>
        <property type="gene ID" value="Nwi_0958"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0958"/>
    <dbReference type="eggNOG" id="ENOG50347MQ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114827_0_0_5"/>
    <dbReference type="OMA" id="RWAVAMA"/>
    <dbReference type="OrthoDB" id="2027719at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="179" mass="19209" checksum="C175E7B501E04FA2" modified="2005-10-11" version="1">MLRWAVAMAEVRRGRRAAVTTIGPLVERSRASLNGIPDTAWRDPYLAGFMLTLITIVARIGCRDLQDHDLSLVQSQAWGAITGMDADLIGEDALTLSSSHPREFQHGCYTAMMVATRLCDPGAVASIGYEPWQITEALPEADGLMGDAHNIGTALSPATGNWVDAFDAYIAGLPLAKPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU27</accession>
    <name>Q3SU27_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Universal stress protein, UspA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0952</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314255.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04214">
        <property type="protein sequence ID" value="ABA04214"/>
        <property type="gene ID" value="Nwi_0952"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0952"/>
    <dbReference type="eggNOG" id="COG0589">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_5_2_5"/>
    <dbReference type="OMA" id="ACKREGV"/>
    <dbReference type="OrthoDB" id="2040681at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="264" mass="28642" checksum="C296F415893377FB" modified="2005-10-11" version="1">MFKDIILHLERDPSRDIARDFGVSIAEAFSAHLTALIFNFEANIPADALGDLFAESEAEARGAIERFELATKLGALSAEPRLVSGTPKTFSEIARCFDLSIVMQSDDHKGINNSILIETTLFDSGRPLIIVPSTQRETLKLEKVVCCWDGSRTAARAINDALPFLKKASAVELLIVEDEKTANEKVHGTEIGRHLARHDIKVGVETIPAADMDIANAILSHVAGSPTSLLVMGGYGHSRLREFVFGGATRGMLSAMTVPVFMSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU75</accession>
    <name>Q3SU75_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alginate_exp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0904</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0904"/>
    <dbReference type="EnsemblBacteria" id="ABA04166">
        <property type="protein sequence ID" value="ABA04166"/>
        <property type="gene ID" value="Nwi_0904"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0904"/>
    <dbReference type="eggNOG" id="COG3637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040635_0_0_5"/>
    <dbReference type="OMA" id="MMSHRAY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025388">
        <property type="entry name" value="Alginate_export_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13372">
        <property type="entry name" value="Alginate_exp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229213" description="Alginate_exp domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="Alginate_exp" evidence="3">
        <location>
            <begin position="117"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="498" mass="55107" checksum="3732DC76E8EA4914" modified="2005-10-11" version="1">MKRILLSGVVLAAIPQNLAHAQGDEGQGRGHGHAIAQNGDAHTDAQPQTWPAFKATTSKPVFSLYDALGRPDNLTVKGVFRARGEGLANTFRPAPFAHNDYLASFFTDIFAEYNLGTVKIGAEVFDSRGYFVPRNSAVSTTDVNAFQLGQAYLNVDLTGILQDGSRSSLTAGRFTKDMGARRLISRPIYRNTMNAFTGLSYDWKGANTDRMTLLWTMPHRLLPDDTRGIHNNTIELDSESPDLQLFGGSYTFAKVFGGSFEVYGYGLYEQDSGTGPGSVQTRNRRLFIPGVRLARKKKAGELDYDFEAIYQTGLSRETTAVTDTRDLNVSAFFVHGEAGYTFAANWQPRVALQYDHASGDSSNPNTFNRFDTLYGVAREYNPMAIYRPVGRANLVSPAIRFEVTPSPIWDAFVAYRALFLADRTDSFGGTQIRDRSGRSGNFAGQQVEARLRYWVVPDAMLLETGAAYLFKGSFLRDAPNAPDTADTFYGYLQTSFFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU84</accession>
    <name>Q3SU84_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0893</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AHA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314198.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04157">
        <property type="protein sequence ID" value="ABA04157"/>
        <property type="gene ID" value="Nwi_0893"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0893"/>
    <dbReference type="eggNOG" id="COG3832">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108923_8_0_5"/>
    <dbReference type="OMA" id="EMCYLGW"/>
    <dbReference type="OrthoDB" id="1914312at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013538">
        <property type="entry name" value="Activator_of_Hsp90_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08327">
        <property type="entry name" value="AHSA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="147" mass="16266" checksum="AEC29F51D0544CB6" modified="2005-10-11" version="1">MPSTIRLHRVIAAKPEKVYRAFVESDAVASWLPPYGFLCTVQELEAKVGGKHRMSFRNFTTGANHSFGGTYVELVPNKRLVYTDSFDDPNLPGEMRVTVTLKAVSVGTEINIEQTGVPDVIPPEGCYLGWQDSLDKLARLVGPEINE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU92</accession>
    <name>Q3SU92_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0885</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314190.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0885"/>
    <dbReference type="EnsemblBacteria" id="ABA04149">
        <property type="protein sequence ID" value="ABA04149"/>
        <property type="gene ID" value="Nwi_0885"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0885"/>
    <dbReference type="eggNOG" id="ENOG50316NY">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032638_0_0_5"/>
    <dbReference type="OrthoDB" id="1300128at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010567">
        <property type="entry name" value="Clostridium_P47"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06597">
        <property type="entry name" value="Clostridium_P47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="423"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="455"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="530" mass="56652" checksum="2F25C8F4D767F4C5" modified="2005-10-11" version="1">MDARVLEAHDKPLVFSFPDLDEDLRIEALEKHDRHGAAFLAAQSGGSNPPPKADTLGWDTVFAIRMTDVNKAIVKQKTTPPTFSVSNPLGEGSSLSGNFAAWQLTTGADGENVNVLVPASSGTFIFGGKNYDLTGVTATAQYHLNVIPPNAPPTKPTETGTKHNIVVSDQAPGPNVSPVSVVFIDVPASTSAKWPDGMSIEAVKGLLLAAMTTYLNANIKDFTQTFAAVDLNIMADQGTLQWLSPALTSYAFADGGTVENSFFGVLTLTSDIGRAENLKHELAASAIPTNQRSAFLISQQLFLREAIFPGLPHAFNNARTSDFRLVNNDTEIINNGSGKVELDSVKVGAIDYHPYIESFDLVINTTEFHTTMEVKVNISPGIDTYITINTWHGLKLEKKTNGKQTIGFSETRPYSATHRVHTAPGVIITEVIAGIIVSVVGLVVGKIVDTLAKRIIIAIIVAIIAGIITAIQLIITEVIAKGVAESMPEIDPLVQTGTSPITWPTQQSQFTITAVQLNESVQLSGDPGFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUC9</accession>
    <name>Q3SUC9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0848</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0848"/>
    <dbReference type="EnsemblBacteria" id="ABA04112">
        <property type="protein sequence ID" value="ABA04112"/>
        <property type="gene ID" value="Nwi_0848"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0848"/>
    <dbReference type="eggNOG" id="ENOG502Z7UG">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123182_0_0_5"/>
    <dbReference type="OMA" id="YTVEQHR"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021263">
        <property type="entry name" value="DUF2840"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11000">
        <property type="entry name" value="DUF2840"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="106" mass="12221" checksum="F9EE82E3590CAD50" modified="2005-10-11" version="1">MRWAANEYGTIISRIDIVRSVEGGEPYQTLPFVRPGGDILLRISGWPKVQRVLAAIDAIEAIGIDPPDVAPEHWRHLNHRIGVNEPFRIYSHEQHHAWLLRREVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUJ4</accession>
    <name>Q3SUJ4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Integrase, catalytic region</fullName>
            <ecNumber evidence="2">2.7.7.49</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0782</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04047">
        <property type="protein sequence ID" value="ABA04047"/>
        <property type="gene ID" value="Nwi_0782"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0782"/>
    <dbReference type="eggNOG" id="COG2801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027402_10_0_5"/>
    <dbReference type="OMA" id="MYSPMER"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="43"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="237" mass="27426" checksum="86694E3ED39807B9" modified="2005-10-11" version="1">MRFTDEKRYFVSEASVYRLLKAHDLITSPAYIVIKAASEFKDKTTAPNQLWQTDFTYLKITGWGWYYLSTVLDDFSRFIVAWKLCATMRADDVTATLDLALAASGLDQITVAHRPRLLSDNGASYISAELATWLDGKGMKHVRGAPYHPQTQGKIERWHQTLKNRILLENYYLPGDLERQVAAFVEHYNHGRYHESIDNLTPADVYFGRGQTILTERERIKRQTIHQRRLQHHLQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUM7</accession>
    <name>Q3SUM7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_0749</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314068.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0749"/>
    <dbReference type="EnsemblBacteria" id="ABA04014">
        <property type="protein sequence ID" value="ABA04014"/>
        <property type="gene ID" value="Nwi_0749"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0749"/>
    <dbReference type="eggNOG" id="COG0642">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0784">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_15_5"/>
    <dbReference type="OMA" id="NQLEVQW"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histidine kinase" evidence="6">
        <location>
            <begin position="51"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="7">
        <location>
            <begin position="296"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="4">
        <location>
            <position position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA04014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="418" mass="43711" checksum="B30DEFA86078472B" modified="2005-10-11" version="1">MAPTSRGLRKPRKPTRRPAKKHVPKTPAKPAKAGRPRSQSTSGVVEDALAAFAHEVRTPLTGILAVSDLLATSDLGERERRWVETIKAGADHLASLATLFVDAARSRRSAPGGRQDFFDLRALARNAGDSLAGRATAKGLRSTVEISDGLPAFVTGDAVRLRAALENLIDNAVKFTEQGDVILKVTASRPSEGKTGVTFVISDSGIGLSLTEIRRLFRPYSQANVSIASRFGGAGLGLSSVKQLARAMGGDVTATQRRGGGTTFTFTVLLTPAEGPVAIQSGDDLHRPSDVSGALRILSVEDNPFGRVVLNTILTELGHQAEFVGRGEDAVQRIAQGGFDAVLMDMVLPGITGIEAIGRIRALDAPGGRVAIIGVSGRSDDEAASRAAGGDAFLVKPVSPRALATALLEAKHRAATAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUQ6</accession>
    <name>Q3SUQ6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Assimilatory nitrite reductase (NAD(P)H) large subunit</fullName>
            <ecNumber evidence="17">1.7.1.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">Nwi_0719</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nitrogen metabolism; nitrate reduction (assimilation).</text>
    </comment>
    <dbReference type="EC" id="1.7.1.4" evidence="17"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314039.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0719"/>
    <dbReference type="EnsemblBacteria" id="ABA03985">
        <property type="protein sequence ID" value="ABA03985"/>
        <property type="gene ID" value="Nwi_0719"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0719"/>
    <dbReference type="eggNOG" id="COG1251">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003291_0_0_5"/>
    <dbReference type="OMA" id="FAQVDPW"/>
    <dbReference type="OrthoDB" id="1128731at2"/>
    <dbReference type="UniPathway" id="UPA00653"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008942">
        <property type="term" value="F:nitrite reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042128">
        <property type="term" value="P:nitrate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.413.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007419">
        <property type="entry name" value="BFD-like_2Fe2S-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041854">
        <property type="entry name" value="BFD-like_2Fe2S-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005117">
        <property type="entry name" value="NiRdtase/SiRdtase_haem-b_fer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036136">
        <property type="entry name" value="Nit/Sulf_reduc_fer-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012744">
        <property type="entry name" value="Nitri_red_NirB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017121">
        <property type="entry name" value="Nitrite_Rdtase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006067">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045854">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006066">
        <property type="entry name" value="NO2/SO3_Rdtase_FeS/sirohaem_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041575">
        <property type="entry name" value="Rubredoxin_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04324">
        <property type="entry name" value="Fer2_BFD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01077">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03460">
        <property type="entry name" value="NIR_SIR_ferr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18267">
        <property type="entry name" value="Rubredoxin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037149">
        <property type="entry name" value="NirB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00397">
        <property type="entry name" value="SIROHAEM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55124">
        <property type="entry name" value="SSF55124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56014">
        <property type="entry name" value="SSF56014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02374">
        <property type="entry name" value="nitri_red_nirB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00365">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 11" id="KW-0274">FAD</keyword>
    <keyword evidence="5 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0349">Heme</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11" id="KW-0534">Nitrate assimilation</keyword>
    <keyword evidence="7 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="15">
        <location>
            <begin position="6"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="Rubredoxin_C" evidence="16">
        <location>
            <begin position="317"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Fer2_BFD" evidence="14">
        <location>
            <begin position="421"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="Fer2_BFD" evidence="14">
        <location>
            <begin position="484"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="NIR_SIR_ferr" evidence="13">
        <location>
            <begin position="559"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="domain" description="NIR_SIR" evidence="12">
        <location>
            <begin position="633"/>
            <end position="767"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR037149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF18267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ABA03985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="820" mass="89380" checksum="095B2057BE935DF4" modified="2005-10-11" version="1">MNKKEKLVIVGAGMASGRMLEHLFEADPERFDVTLFGAEPRGNYNRIMLSPVLAGEKSFDQIVTHDAAWYDAHQVECRFGETVTRIDRAAKVVHSAGSEAPYDKLVIATGSAPFMIPVAGRDLPGVMAFRDYDDVQAMVAAAGKKDAKAVVIGGGLLGLEAAAALRLHGMEVVVLHLMGHLMERQLDAAAGYLLQKELESRGIKIHCKAQTSAILGRGRTEAVLLDDGTLYPADIVVMAVGIRPEARIATDAGLHVERGIVVDDRMLTSDPDILALGECCEHNNICYGLVAPLYDMAKVAAKTLTGVEATFRSVDTATQLKVTGVSLYSAGDFADAPDREDIVLRNSGAGIYKRLVLKDNRIVGVVLYGDTTDGAWYFDMLRKGTDVSELRETLIFGQSRQGNASLDPMAAVAALPDDAEICGCNGVCKGAIVSAIIARDLTSLDDVRAHTKASASCGSCTGLVEQLMTLTLGDKYNPDAVQPVCGCTTLGHGDVRRLIKAKRLKTIPAVMQELEWKTSCGCAKCRPALNYYLVCDWPDEYADDYQSRFVNERVHANIQKDGTYSVVPRMWGGVTSAKELRAIADVVDKFHIPSVKVTGGQRIDMLGVRKEDLPAVWADLGRAGFISGHAYAKGLRTVKTCVGSDWCRFGTQDSTGLGIRIEKFMWGAWTPAKVKMAVSGCPRNCAEATCKDVGVICVDSGYEIHFAGAAGLDIRGTEVLGLVKTEDDALEHIVALVQMYREQAHYLERIYKWAKRVGLDEIRRQILDDPQKRKAYFDRFAFSRKFAQVDPWSERVSGKDKHEFRPMATIGFPVAAEEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUQ7</accession>
    <name>Q3SUQ7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0718</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0718"/>
    <dbReference type="EnsemblBacteria" id="ABA03984">
        <property type="protein sequence ID" value="ABA03984"/>
        <property type="gene ID" value="Nwi_0718"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0718"/>
    <dbReference type="HOGENOM" id="CLU_2181104_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="109" mass="11861" checksum="44113DE0512C356C" modified="2005-10-11" version="1">MEAGGAESLCNAAAKATHNPQSGNPSAFDRKRKSFRNQSRTEPIDPGNPKETSMTNPNEKNQDDQRNQGKKPGEQGESTRKSEIKKAVKEDMPDKNPQGQEKTGYSGGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUU9</accession>
    <name>Q3SUU9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PBS lyase HEAT-like repeat</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0676</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313998.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0676"/>
    <dbReference type="EnsemblBacteria" id="ABA03942">
        <property type="protein sequence ID" value="ABA03942"/>
        <property type="gene ID" value="Nwi_0676"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0676"/>
    <dbReference type="eggNOG" id="COG1413">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070753_0_0_5"/>
    <dbReference type="OMA" id="PDYGIRA"/>
    <dbReference type="OrthoDB" id="1113913at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004155">
        <property type="entry name" value="PBS_lyase_HEAT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00567">
        <property type="entry name" value="EZ_HEAT"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="SSF48371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="320" mass="33592" checksum="1FDE0F5AD1F2A4B4" modified="2005-10-11" version="1">MRHPEPFEDFGDVDEIARGLSSSDASVRRLAVIELAETSNSAAVSFLSRATADSSSDVRLQAARALGEFDGSDVAEALVQAVTDPVPDVARAAADSLAELRDPEVSEVLLPFVSHGSSFVRAAIFRALKALQVNASLSSAVVALADSDAAVRAQAVGVIGYLKRLDTLPSLIHASKDASASVRLAAISALSFARNELAAQASADALADDDWQVRAAAAETIGRVGSPSTSETLIRSLDDSYWQVRQKILQSLGRLGTPDAVPAIVPLLADDVPSVRKEAAAALGEISEPSAREALEEHALDPDPDVRKTVLWALNRLGPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV01</accession>
    <name>Q3SV01_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">17kDa_Anti_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0623</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0623"/>
    <dbReference type="EnsemblBacteria" id="ABA03890">
        <property type="protein sequence ID" value="ABA03890"/>
        <property type="gene ID" value="Nwi_0623"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0623"/>
    <dbReference type="eggNOG" id="COG4520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129681_1_0_5"/>
    <dbReference type="OMA" id="NGRPVKW"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032635">
        <property type="entry name" value="Anti_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16998">
        <property type="entry name" value="17kDa_Anti_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="17kDa_Anti_2" evidence="2">
        <location>
            <begin position="16"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="128" mass="13360" checksum="27B5522DBCFF3C98" modified="2005-10-11" version="1">MIGATAGGCSVARNHAAFAQMEDGEATGSIAARAHAGPTDTDLAFARTAASDVLTRGSKDASQPWENPSTGARGSVTPLAQAYTAEGRTCRDFLASYVNEKSESWLQGAACKARGGQWEIRSLKPLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV09</accession>
    <name>Q3SV09_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_0615</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NodD regulates the expression of the nodABCFE genes which encode other nodulation proteins. NodD is also a negative regulator of its own expression. Binds flavonoids as inducers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313942.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0615"/>
    <dbReference type="EnsemblBacteria" id="ABA03882">
        <property type="protein sequence ID" value="ABA03882"/>
        <property type="gene ID" value="Nwi_0615"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0615"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_16_2_5"/>
    <dbReference type="OMA" id="GQAYYRH"/>
    <dbReference type="OrthoDB" id="750230at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0536">Nodulation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA03882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="312" mass="34528" checksum="1C264A3796F74F5D" modified="2005-10-11" version="1">MHDLNDLYFFVQVVDHGGFAAAARALGIPKSRLSRRMATLEARLGVRLIQRSTRRFTVTGIGQDYYRHCVAMLVEAEAAQDLIERSRTKPQGIVRISCPPPLLYFQVGEMIARFMAECPDVEVHLESTPRRVDVIADGIDIALRVRFPPLEDNDLVMRVLAESAQRLVASPSLLKKLSHPPVPADLAHLPSIGWQSPHHPHEWSLDGPDAGTARIPHTPRFITEDMVALRFAALRGIGVGQFPTMMVKADLESGALVDVLPDWTPRAGIVHAVFSSRRGLLPSVRALLDFLAAEFAVLTRADADISPPHKMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV44</accession>
    <name>Q3SV44_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Gamma-glutamylcyclotransferase</fullName>
            <ecNumber evidence="1">4.3.2.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the cleavage of glutathione into 5-oxo-L-proline and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on other gamma-glutamyl peptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>glutathione = 5-oxo-L-proline + L-cysteinylglycine</text>
            <dbReference type="Rhea" id="RHEA:47724"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58402"/>
            <dbReference type="ChEBI" id="CHEBI:61694"/>
            <dbReference type="EC" id="4.3.2.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the gamma-glutamylcyclotransferase family.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.7" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313908.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0580"/>
    <dbReference type="EnsemblBacteria" id="ABA03847">
        <property type="protein sequence ID" value="ABA03847"/>
        <property type="gene ID" value="Nwi_0580"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0580"/>
    <dbReference type="eggNOG" id="COG3703">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070703_1_1_5"/>
    <dbReference type="OMA" id="LLWYTDF"/>
    <dbReference type="OrthoDB" id="1589994at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003839">
        <property type="term" value="F:gamma-glutamylcyclotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061928">
        <property type="term" value="F:glutathione specific gamma-glutamylcyclotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06661">
        <property type="entry name" value="GGCT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006840">
        <property type="entry name" value="ChaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013024">
        <property type="entry name" value="GGCT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036568">
        <property type="entry name" value="GGCT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12192">
        <property type="entry name" value="PTHR12192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04752">
        <property type="entry name" value="ChaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110857">
        <property type="entry name" value="SSF110857"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="194" mass="22218" checksum="F18B07103A6F7D33" modified="2005-10-11" version="1">MSVQRQFETAFDSSGNDLWIFAYGSLMWRPGFDFAEQVPARLIGEHRALCVYSFDHRGTPERPGLVLGLDRGGACRGIAFRVAASRREATIAYLREREQTTHVYREVTRSVWLEDDARRRVSALAYVVDRGHVQYAGRLTLADQARYVRQGHGRSGHNRDYVLSTVRSIESQGFRDLKLHQLAELLHNDTPMPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV84</accession>
    <name>Q3SV84_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0540</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313868.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03807">
        <property type="protein sequence ID" value="ABA03807"/>
        <property type="gene ID" value="Nwi_0540"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0540"/>
    <dbReference type="eggNOG" id="ENOG50330CT">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_725279_0_0_5"/>
    <dbReference type="OMA" id="WNENGEG"/>
    <dbReference type="OrthoDB" id="1006904at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="381" mass="42512" checksum="FF1AE7CAB8373F56" modified="2005-10-11" version="1">MLTRRAVVASLAANLFPPIFGRTSFAVKPRPLVGAIRWDAWYSPGSAPTEAVENSLSPEKYRWRAPFFARGSAHDPASLSFPPITPEEMDKEIRQAAFAGLDYWAFVAYGSHHPMSKALYQFRASAESDKLGYCLFTELDRWGSRNKPTTLLQEHIGLMADRNYLRVSGDRPLYFLGFIAPASIERNWKGVAGLREQIEKFRSMAAVSGLADPYIVLAGDRNFLMREARNIGGDATGSYALTVGNGRGSFSDLTRIAERGWNELGNGHLPVVPTVMTGWDRRPRIENPVPWEKKQRPGEGIENFFAAPTKKELADHLARALDWVGARPQGEQAPVVLIYAWNENDEGGWLMPTLPCQTDRLDALRQVLKKTAAPVRKPELC</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-01-23" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVB5</accession>
    <name>PSTB_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphate import ATP-binding protein PstB</fullName>
            <ecNumber evidence="1">7.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ABC phosphate transporter</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphate-transporting ATPase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pstB</name>
        <name type="ordered locus">Nwi_0509</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the ABC transporter complex PstSACB involved in phosphate import. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + phosphate(out) = ADP + H(+) + 2 phosphate(in)</text>
            <dbReference type="Rhea" id="RHEA:24440"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.3.2.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of two ATP-binding proteins (PstB), two transmembrane proteins (PstC and PstA) and a solute-binding protein (PstS).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. Phosphate importer (TC 3.A.1.7) family.</text>
    </comment>
    <dbReference type="EC" id="7.3.2.1" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313837.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVB5"/>
    <dbReference type="SMR" id="Q3SVB5"/>
    <dbReference type="STRING" id="323098.Nwi_0509"/>
    <dbReference type="EnsemblBacteria" id="ABA03776">
        <property type="protein sequence ID" value="ABA03776"/>
        <property type="gene ID" value="Nwi_0509"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0509"/>
    <dbReference type="eggNOG" id="COG1117">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_5"/>
    <dbReference type="OMA" id="VKHALQG"/>
    <dbReference type="OrthoDB" id="1416748at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015415">
        <property type="term" value="F:ATPase-coupled phosphate ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03260">
        <property type="entry name" value="ABC_PstB_phosphate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015850">
        <property type="entry name" value="ABC_transpr_PstB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005670">
        <property type="entry name" value="Phosp_transpt1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43423">
        <property type="entry name" value="PTHR43423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43423:SF3">
        <property type="entry name" value="PTHR43423:SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00972">
        <property type="entry name" value="3a0107s01c2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51238">
        <property type="entry name" value="PSTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0592">Phosphate transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000272483" description="Phosphate import ATP-binding protein PstB">
        <location>
            <begin position="1"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="27"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="59"/>
            <end position="66"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="273" mass="30372" checksum="5360D7259E5B9FBC" modified="2005-10-11" version="1">MTTVSTAAASGPAVPPPRIDPDVPAKVAARNLNFYYGQHHALKNINLSIAANRVTAFIGPSGCGKSTLLRIFNRMYDLYPGQRVEGRVLLDDTDILDPKLDLNLLRARVGMVFQKPTPFPMTIYENIAFGIRLYEKLPKSEMDSRVEKALRDGALWTEVKDKLNASGLSLSGGQQQRLCIARTVAVRPEVILFDEPCSALDPISTAKVEELIDELKTHYTIAIVTHNMQQAARVSDYTSFMYLGEMIEFGPTDKLFTSPSDRRTQDYITGRFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVD9</accession>
    <name>Q3SVD9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GCN5-related N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0485</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0485"/>
    <dbReference type="EnsemblBacteria" id="ABA03752">
        <property type="protein sequence ID" value="ABA03752"/>
        <property type="gene ID" value="Nwi_0485"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0485"/>
    <dbReference type="eggNOG" id="COG1247">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_18_2_5"/>
    <dbReference type="OMA" id="QTWREAY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013653">
        <property type="entry name" value="FR47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08445">
        <property type="entry name" value="FR47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="20"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="183" mass="20516" checksum="A4CE813569D59391" modified="2005-10-11" version="1">MDLEDRAPTCFDRHMSTTLIELRPAKVADASAVAATHDESWRSAYQGIIPGAELEKLINRRGPQWWDSAIRKGSRISVLVFGEQVAGYANYGRNRARSLQYDGEVYELYLRPEFQGLGFGRRLFAAARRDLVQSGLKSVVTWALSDNEPAMEFYCALGGRMIARSSERFGAKSLDKVAFAWTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVE7</accession>
    <name>Q3SVE7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Short-chain dehydrogenase/reductase SDR</fullName>
            <ecNumber evidence="2">1.1.1.100</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0477</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.100" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0477"/>
    <dbReference type="EnsemblBacteria" id="ABA03744">
        <property type="protein sequence ID" value="ABA03744"/>
        <property type="gene ID" value="Nwi_0477"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0477"/>
    <dbReference type="eggNOG" id="COG0300">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_1_5"/>
    <dbReference type="OMA" id="FQPGPWM"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="256" mass="26920" checksum="B841F25292CE9C58" modified="2005-10-11" version="1">MTLITGASSGIGAEFARVFAAHGHRLALVARRADRLNALAAEIVASGRMAPIVIPCDLGEKGAAERIAAALTAEGVEVKYLVNNAGYGLSGHAVELDHADQLALIDLNVRALTDLSLRFSESLIRLRGGILNVGSIAGFLPGPGMAVYYASKAYVLSFSEALRGELGPRGVHVTVVCPGPVPTEFQARAGVKSGFHASILQVSARDVALEGYRGLMANKRAVLPGLGVKMLPLLLRLVPRDAILRAVAGFQARRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVE9</accession>
    <name>Q3SVE9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0475</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313806.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0475"/>
    <dbReference type="EnsemblBacteria" id="ABA03742">
        <property type="protein sequence ID" value="ABA03742"/>
        <property type="gene ID" value="Nwi_0475"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0475"/>
    <dbReference type="eggNOG" id="ENOG50314FI">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145244_5_0_5"/>
    <dbReference type="OMA" id="DPGCGRD"/>
    <dbReference type="OrthoDB" id="2038885at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001893">
        <property type="entry name" value="Cys-rich_GLG1_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00839">
        <property type="entry name" value="Cys_rich_FGFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229265" evidence="1">
        <location>
            <begin position="23"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="76" mass="8119" checksum="A8B905DC51D99F88" modified="2005-10-11" version="1">MIRFAVVLSLLPLLAVTAVASAQTAAEKHNSGCARDVAKYCRAQMNDGDMVVLSCLKAHRAKLSKKCARTLAVHGH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-11-28" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVH7</accession>
    <name>RLMH_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal RNA large subunit methyltransferase H</fullName>
            <ecNumber evidence="1">2.1.1.177</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">23S rRNA (pseudouridine1915-N3)-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">23S rRNA m3Psi1915 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">rRNA (pseudouridine-N3-)-methyltransferase RlmH</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rlmH</name>
        <name type="ordered locus">Nwi_0447</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>pseudouridine(1915) in 23S rRNA + S-adenosyl-L-methionine = H(+) + N(3)-methylpseudouridine(1915) in 23S rRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10221"/>
            <dbReference type="Rhea" id="RHEA-COMP:10222"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:74486"/>
            <dbReference type="EC" id="2.1.1.177"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA methyltransferase RlmH family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.177" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313778.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVH7"/>
    <dbReference type="SMR" id="Q3SVH7"/>
    <dbReference type="STRING" id="323098.Nwi_0447"/>
    <dbReference type="EnsemblBacteria" id="ABA03714">
        <property type="protein sequence ID" value="ABA03714"/>
        <property type="gene ID" value="Nwi_0447"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0447"/>
    <dbReference type="eggNOG" id="COG1576">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100552_1_1_5"/>
    <dbReference type="OMA" id="NEPYHHQ"/>
    <dbReference type="OrthoDB" id="1783583at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070038">
        <property type="term" value="F:rRNA (pseudouridine-N3-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18081">
        <property type="entry name" value="RlmH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00658">
        <property type="entry name" value="23SrRNA_methyltr_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003742">
        <property type="entry name" value="RlmH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33603">
        <property type="entry name" value="PTHR33603"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02590">
        <property type="entry name" value="SPOUT_MTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004505">
        <property type="entry name" value="MT_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000260576" description="Ribosomal RNA large subunit methyltransferase H">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00658"/>
        </source>
    </evidence>
    <sequence length="160" mass="17710" checksum="0C9C5491CE3FE505" modified="2005-10-11" version="1">MRLVVIAVGRLKQGPERELADRYRGRFADIGRNLGFRGLDVHEVAESRARDAGQRIREEAAAVLALAPKEMILVALDEKGKSIDSAAFAEHLGRWRDESVADAVFMIGGADGLSPELRRRARLSVAFGAATWPHQMVRVMLLEQIYRAATILAGHPYHRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="132" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVT6</accession>
    <name>Q3SVT6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Molybdate ABC transporter, ATP-binding protein</fullName>
            <ecNumber evidence="7">3.6.3.29</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0338</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in beta-(1--&gt;2)glucan export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.29" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313670.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0338"/>
    <dbReference type="EnsemblBacteria" id="ABA03605">
        <property type="protein sequence ID" value="ABA03605"/>
        <property type="gene ID" value="Nwi_0338"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0338"/>
    <dbReference type="eggNOG" id="COG4148">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_5"/>
    <dbReference type="OMA" id="HPPVYAP"/>
    <dbReference type="OrthoDB" id="1200451at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015098">
        <property type="term" value="F:molybdate ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011868">
        <property type="entry name" value="ModC_ABC_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02142">
        <property type="entry name" value="modC_ABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="1"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="30"/>
            <end position="37"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="221" mass="24677" checksum="DCADF94CED3E939C" modified="2005-10-11" version="1">MLRVEVSKQLGQFSVDVAFASEGRVTGLFGPSGSGKTSLVSMIAGLMTPDRGVISVDGEALDDTTRRFHVAVHRRRIGYVFQDARLFPHFDVRRNLDYGRRMNRLTRDDAQEKRLSEMLNIGHLLDRRPGQLSGGERQRVALGRALLAQPRLLLLDEPLGSLDEDRKAEILPYLTRLRDEAGVPMVYVSHDPDELRQLASHIVMLKRGRVAAFGGPEVLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVV0</accession>
    <name>Q3SVV0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0324</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0324"/>
    <dbReference type="EnsemblBacteria" id="ABA03591">
        <property type="protein sequence ID" value="ABA03591"/>
        <property type="gene ID" value="Nwi_0324"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0324"/>
    <dbReference type="eggNOG" id="COG5429">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065609_0_0_5"/>
    <dbReference type="OMA" id="LPIDYWD"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010634">
        <property type="entry name" value="DUF1223"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36057">
        <property type="entry name" value="PTHR36057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06764">
        <property type="entry name" value="DUF1223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="278" mass="29910" checksum="8EC93F14A5B65B4A" modified="2005-10-11" version="1">MPASRILKVCFSFMPQAPRRAAVSVILMMSRGGLTRLSGALGVCALVALSRAALAEPRAVVELFTSQGCSSCPAADEILGDLSKDPSIVALSMPIDYWDYLGWKDTLADNRFTARQKAYSQMRGDRDVYTPQVIVNGSAHVIGSDRGAIERAVRDTQQSQDVMSVPVSMSVSGRQLNVSVASANGKHLSGRGEIWVCAVSRAVPIAIGRGENSGREVTYHNVVRNWLKVGDWTGKAGTWAVPIENIAHNGVDAAVVYVQDGNRERPGRMLGAAYTLLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVW3</accession>
    <name>Q3SVW3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cold-shock DNA-binding protein family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0311</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0311"/>
    <dbReference type="EnsemblBacteria" id="ABA03578">
        <property type="protein sequence ID" value="ABA03578"/>
        <property type="gene ID" value="Nwi_0311"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0311"/>
    <dbReference type="eggNOG" id="COG1278">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117621_4_1_5"/>
    <dbReference type="OMA" id="HYSTIKM"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012156">
        <property type="entry name" value="Cold_shock_CspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019844">
        <property type="entry name" value="CSD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002599">
        <property type="entry name" value="Cold_shock_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00050">
        <property type="entry name" value="COLDSHOCK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00352">
        <property type="entry name" value="CSD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CSD" evidence="3">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="68" mass="7287" checksum="7259A68C394A16A7" modified="2005-10-11" version="1">MGTVKWFNATKGYGFIQPDDGGNDVFVHISAVERAGLGTLREGQKISYEIVADRRSGKSSADNLRAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVY2</accession>
    <name>Q3SVY2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC transporter, ATPase subunit</fullName>
            <ecNumber evidence="9">3.6.3.25</ecNumber>
            <ecNumber evidence="9">3.6.3.29</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_0292</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in beta-(1--&gt;2)glucan export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.25" evidence="9"/>
    <dbReference type="EC" id="3.6.3.29" evidence="9"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313625.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0292"/>
    <dbReference type="EnsemblBacteria" id="ABA03559">
        <property type="protein sequence ID" value="ABA03559"/>
        <property type="gene ID" value="Nwi_0292"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0292"/>
    <dbReference type="eggNOG" id="COG4172">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_86_2_5"/>
    <dbReference type="OMA" id="RVCVMQR"/>
    <dbReference type="OrthoDB" id="1303270at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="11"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="290"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="45"/>
            <end position="52"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="322"/>
            <end position="329"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA03559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="545" mass="59573" checksum="6463428BBB2EDB4F" modified="2005-10-11" version="1">MDAIDQPLLDVRDLSVAFHHGGGTSVAVDRISFDIKRGECVALVGESGSGKSVSALSVLKLLPYPAASHPSGHIRFNGRELLGLSEREIRSIRGNDISIIFQEPMTSLNPLHTIEAQIGEILLLHGGVRGAAARKRTLELLTQVGIPEPETRLPSYPHQLSGGQRQRVMIAMALANEPDLLIADEPTTALDVTVQAQILALLAEIRARLGMSLLFITHDLGIVRRIADRVCVMNGGKIVEQGPVEQVFESPQHPYTRELLAAEPKPDPAPPRPDAPVVIATDNLKVWFPIRRGLLRKTIGHIKAVDGVTLKVRQGETLGVVGESGSGKTTLGLALLRLISSDGPIVFLGKDIQGLRFKEMRPFRRDMQIVFQDPFGALSPRMSVGDIVAEGLSVHQGSLSETEREARVVKALTDVGLDPATRFRYPHEFSGGQRQRISIARAVVLEPNFVVLDEPTSALDMLFQAQMVDLLRELQRKRDLTYMFISHDLRVVASLASHLIVMRQGKVVEEGPASELFRNPQSDYTRALFAAAFRLETAPGEAGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW08</accession>
    <name>Q3SW08_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0266</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313599.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0266"/>
    <dbReference type="EnsemblBacteria" id="ABA03533">
        <property type="protein sequence ID" value="ABA03533"/>
        <property type="gene ID" value="Nwi_0266"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0266"/>
    <dbReference type="eggNOG" id="COG2405">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090263_0_0_5"/>
    <dbReference type="OMA" id="INLHACK"/>
    <dbReference type="OrthoDB" id="1756758at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="205" mass="21538" checksum="663FEBAB60BE85F8" modified="2005-10-11" version="1">MSSSSFPIDAQTVLVADASVAINLNATGCALEIIRAQPGAVVITENALAELAEGRRNGHRDYDQIQALIAANAMQVFRLDESGNKVYASLVDGTAIRTLDDGEAATIGFACQTGAIALIDERKAQSICAADFPALRVVSTVDFLTHSLVADALGESALAQAIVNALRNARMRVPPHQMEMIVKLIGEEAALNCTSLPKAHRAAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW82</accession>
    <name>Q3SW82_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Heme chaperone HemW</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_0191</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Probably acts as a heme chaperone, transferring heme to an unknown acceptor. Binds one molecule of heme per monomer, possibly covalently. Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the anaerobic coproporphyrinogen-III oxidase family. HemW subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313527.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0191"/>
    <dbReference type="EnsemblBacteria" id="ABA03459">
        <property type="protein sequence ID" value="ABA03459"/>
        <property type="gene ID" value="Nwi_0191"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0191"/>
    <dbReference type="eggNOG" id="COG0635">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027579_0_1_5"/>
    <dbReference type="OMA" id="HIPWCVR"/>
    <dbReference type="OrthoDB" id="1130951at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004109">
        <property type="term" value="F:coproporphyrinogen oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006779">
        <property type="term" value="P:porphyrin-containing compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034505">
        <property type="entry name" value="Coproporphyrinogen-III_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010723">
        <property type="entry name" value="HemN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004559">
        <property type="entry name" value="HemW-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13932">
        <property type="entry name" value="PTHR13932"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06969">
        <property type="entry name" value="HemN_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00562">
        <property type="entry name" value="HemN-like__clustered_with_heat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00539">
        <property type="entry name" value="hemN_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9 10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 10" id="KW-0349">Heme</keyword>
    <keyword evidence="7 10" id="KW-0408">Iron</keyword>
    <keyword evidence="8 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="11">
        <location>
            <begin position="4"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA03459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="386" mass="42362" checksum="CAF20527913C2E23" modified="2005-10-11" version="1">MSPADPAAAFGVYIHWPFCLSKCPYCDFNSHVRHAPIDEERFTRAFAREIETTAARSRGRTVSSIFLGGGTPSLMRPQTVGAILDAIGKHWSVADDVEVTLEANPTSVEATRFRGYRAAGVNRVSLGVQALDDSSLKALGRLHTAREAQDAVAIARSVFDRYSFDLIYARPDQTPAMWAGELQRAISEAAEHLSLYQLTIEAGTPFFDLHAAGKLKTPDEAMARDLYDVTQDVCARHGLPAYEISNHARPGAECRHNLVYWRGQEYAGIGPGAHGRLDIDGVRHAIATEKRPEAWLMRVEANGNGIVADDPLNSEERADEFLLMGLRLREGIDPRRYAALSGRSLDPRRIAILRDEGAIMISTDGRLRVTQDGFPVLDAVVADLAA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-21" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWA8</accession>
    <name>UPPP_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Undecaprenyl-diphosphatase</fullName>
            <ecNumber evidence="1">3.6.1.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Bacitracin resistance protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Undecaprenyl pyrophosphate phosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">uppP</name>
        <name type="ordered locus">Nwi_0165</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the dephosphorylation of undecaprenyl diphosphate (UPP). Confers resistance to bacitracin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>di-trans,octa-cis-undecaprenyl diphosphate + H2O = di-trans,octa-cis-undecaprenyl phosphate + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:28094"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:60392"/>
            <dbReference type="EC" id="3.6.1.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text>Bacitracin is thought to be involved in the inhibition of peptidoglycan synthesis by sequestering undecaprenyl diphosphate, thereby reducing the pool of lipid carrier available.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UppP family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.27" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313502.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWA8"/>
    <dbReference type="SMR" id="Q3SWA8"/>
    <dbReference type="STRING" id="323098.Nwi_0165"/>
    <dbReference type="EnsemblBacteria" id="ABA03433">
        <property type="protein sequence ID" value="ABA03433"/>
        <property type="gene ID" value="Nwi_0165"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0165"/>
    <dbReference type="eggNOG" id="COG1968">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060296_2_0_5"/>
    <dbReference type="OMA" id="IEGPFRD"/>
    <dbReference type="OrthoDB" id="1826154at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050380">
        <property type="term" value="F:undecaprenyl-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016311">
        <property type="term" value="P:dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01006">
        <property type="entry name" value="Undec_diphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003824">
        <property type="entry name" value="UppP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30622">
        <property type="entry name" value="PTHR30622"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02673">
        <property type="entry name" value="BacA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00753">
        <property type="entry name" value="undec_PP_bacA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0046">Antibiotic resistance</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0133">Cell shape</keyword>
    <keyword id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000227621" description="Undecaprenyl-diphosphatase">
        <location>
            <begin position="1"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01006"/>
        </source>
    </evidence>
    <sequence length="268" mass="29194" checksum="A8770B4823559324" modified="2005-10-11" version="1">MISDAIRAVILGIIEGVTEFLPVSSTGHLLLAERFFDLGSGNFWDTFTVLIQPGAILAIVVIYFAKLWRVALGMFSNPADRRFVIGVLAAFLPAVIVGLIAGKYIKELLFNPWVVCFSLIVGGAVLMWVDQLDHRPREHDATAFPLPMYVWIGIAQCVAMIPGVSRSGATIVSAMLLGADKRAAAEFSFFLAIPTMTGAFAYDFYKNHADMTTDDLGTVAIGFVVSFVTAIIVVKAFLSYVTRNGFTFFAWWRVIVGTLGLIALALGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWC4</accession>
    <name>Q3SWC4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase, family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0149</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313486.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0149"/>
    <dbReference type="EnsemblBacteria" id="ABA03417">
        <property type="protein sequence ID" value="ABA03417"/>
        <property type="gene ID" value="Nwi_0149"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0149"/>
    <dbReference type="eggNOG" id="COG1215">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029775_0_0_5"/>
    <dbReference type="OMA" id="NCALPFA"/>
    <dbReference type="OrthoDB" id="724641at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037257">
        <property type="entry name" value="T2SS_E_N_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160246">
        <property type="entry name" value="SSF160246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="450" mass="48466" checksum="BBCDAD461CF82A31" modified="2005-10-11" version="1">MGNGGRADALRRAAPGGPPRPPGFDGIPASSNDNWLDDPEPGPAIELDCLRHILAPALLRAAEARGREVATGADQALIRSGVIGEEAYLQALSFQTGLAIETFAEVSRSDCPLPDRYLSSAAQHGLLPLRSRGKLVWIVAPRGFTARRLCRMVAAYPSLCDRILLASRRNLHQFLLRQEAGDVLGRSAAKALHRRFPALSAAPAADDGAHRGLRTMRRLAQTALLSAMMTLTPAIVATDIWSSALALWFLAFIGLRLTASLMPPRPVRRPPRLPDHGLPVYTVIAALYREASSVASLLQAIEALDYPREKLDVIIVIELDDLETRAALARLGPMPHVQVLLAPAEGPRTKPKALNCALPFARGGFTAVFDAEDRPEPGQLRAALDAFRTQGADIACVQASLCIENQSDSWLSRMFAAEYAGQFDVFLPGLASFGVPLPLGGSSNHFRGIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWL2</accession>
    <name>Q3SWL2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0061</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313400.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0061"/>
    <dbReference type="EnsemblBacteria" id="ABA03329">
        <property type="protein sequence ID" value="ABA03329"/>
        <property type="gene ID" value="Nwi_0061"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0061"/>
    <dbReference type="eggNOG" id="COG1225">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127635_0_0_5"/>
    <dbReference type="OMA" id="FEHHEVM"/>
    <dbReference type="OrthoDB" id="1617952at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016209">
        <property type="term" value="F:antioxidant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="1">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="161" mass="17101" checksum="71233FC0DA8C4005" modified="2005-10-11" version="1">MTADAPAPELAVSQWFNTAEPITLAALRGRPVLLHAFQMLCPGCVAHGTPQTQRAHELFKATDLQVIGLHTVFEHHAAMTPVSLQAFIHEYRLTFPIGVDAAGDGTPLPVTMGRYGMQGTPTSILIGRNGRIVHQGFGQQSDMALGAMIAAELAGAQTYAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWP0</accession>
    <name>Q3SWP0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">3-oxoacyl-[acyl-carrier-protein] synthase I</fullName>
            <ecNumber evidence="4">2.3.1.41</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0033</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP synthases family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.41" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313372.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0033"/>
    <dbReference type="EnsemblBacteria" id="ABA03301">
        <property type="protein sequence ID" value="ABA03301"/>
        <property type="gene ID" value="Nwi_0033"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0033"/>
    <dbReference type="eggNOG" id="COG0304">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_69_2_5"/>
    <dbReference type="OMA" id="YAYLSMQ"/>
    <dbReference type="OrthoDB" id="572620at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00834">
        <property type="entry name" value="KAS_I_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000794">
        <property type="entry name" value="Beta-ketoacyl_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712">
        <property type="entry name" value="PTHR11712"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="B_KETOACYL_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PKS_KS" evidence="3">
        <location>
            <begin position="4"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="407" mass="42979" checksum="43BC7AE02B99D5F3" modified="2005-10-11" version="1">MRRVVVTGMGIVSSIGNSTQEVLSSLFEAKSGITRAEKYAELGFRSQVQGAPTLNPADVVDRRAMRFLAEGAAWNHVAMEQAIRDSGLEPNEVSNERTGIIMGSGGPSARTIVEAADITRSKGPKRVGPFAVPKAMSSTASATLATWFKIKGVNYSISSACATSNHCIGNAYETIQIGKQDIIFAGGCEELDWSLSVLFDAMGAMSSKYNDTPATASRAYDVSRDGFVIAGGAGVVVLEELEHAKARGARIYGEVAGYGATSDGYDMVAPSGEGAERCMRMALSTVKTKVDYINPHATSTPAGDPPEIEAIRKVFGAGDKCPPISATKSLTGHSLGATGVQEAIYSLLMMNNGFICESANITELDPVFADMPIVRKRIDDARIGTVMSNSFGFGGTNATLVFKRLDA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMV1</accession>
    <name>RS20_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsT</name>
        <name type="ordered locus">Nwi_3143</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds directly to 16S ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316298.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SMV1"/>
    <dbReference type="SMR" id="Q3SMV1"/>
    <dbReference type="STRING" id="323098.Nwi_3143"/>
    <dbReference type="EnsemblBacteria" id="ABA06390">
        <property type="protein sequence ID" value="ABA06390"/>
        <property type="gene ID" value="Nwi_3143"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3143"/>
    <dbReference type="eggNOG" id="COG0268">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_160655_3_0_5"/>
    <dbReference type="OMA" id="GVIHKNA"/>
    <dbReference type="OrthoDB" id="2005443at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00500">
        <property type="entry name" value="Ribosomal_S20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002583">
        <property type="entry name" value="Ribosomal_S20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036510">
        <property type="entry name" value="Ribosomal_S20_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33398">
        <property type="entry name" value="PTHR33398"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01649">
        <property type="entry name" value="Ribosomal_S20p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46992">
        <property type="entry name" value="SSF46992"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00029">
        <property type="entry name" value="S20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000224974" description="30S ribosomal protein S20">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00500"/>
        </source>
    </evidence>
    <sequence length="88" mass="9905" checksum="461A0831D636A808" modified="2005-10-11" version="1">MANTTSAKKATRKIARRTAINRSRRTLMRGTVRIVEEAIAKGDRDAAIQAMKRAEPELMRAGQQNIIHKNNASRKVSRLTHRIAKLAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMV2</accession>
    <name>Q3SMV2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_3142</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3142"/>
    <dbReference type="EnsemblBacteria" id="ABA06389">
        <property type="protein sequence ID" value="ABA06389"/>
        <property type="gene ID" value="Nwi_3142"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3142"/>
    <dbReference type="HOGENOM" id="CLU_2480209_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="87" mass="9538" checksum="E4ED1E21E28319FF" modified="2005-10-11" version="1">MCFPVFLKTPTSEFTASRGVFFKEQRRQDRPAAIGGLIEPLFSSVNVSPWADAVKTAFSKASKETLARLNFAFLGDKGDLRPLGNGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN14</accession>
    <name>Q3SN14_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fec I like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_3079</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316242.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3079"/>
    <dbReference type="EnsemblBacteria" id="ABA06327">
        <property type="protein sequence ID" value="ABA06327"/>
        <property type="gene ID" value="Nwi_3079"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3079"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_12_3_5"/>
    <dbReference type="OMA" id="RDHHRWL"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="2">
        <location>
            <begin position="9"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="3">
        <location>
            <begin position="109"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA06327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="170" mass="19618" checksum="88D0FFBBA04B27F1" modified="2005-10-11" version="1">MSAALNAIFQSHRRSLLWTVMRIVRDPQTAEDLAQETYLKVRKALETRPIEHVESFLFQTARNLAIDHQRRKAVRNRYEASDAAREDIENVAAAEPSIEDDLIQQQRWRAFEDALAELPVRARTVWSMIHLQGRTCAEIAEHLGVSRNTVYNDIKLVMGHCQDVLARLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN18</accession>
    <name>Q3SN18_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribonuclease P protein component</fullName>
            <shortName evidence="6">RNase P protein</shortName>
            <shortName evidence="6">RNaseP protein</shortName>
            <ecNumber evidence="6">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Protein C5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rnpA</name>
        <name evidence="7" type="ordered locus">Nwi_3075</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Consists of a catalytic RNA component (M1 or rnpB) and a protein subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the RnpA family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316239.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3075"/>
    <dbReference type="EnsemblBacteria" id="ABA06323">
        <property type="protein sequence ID" value="ABA06323"/>
        <property type="gene ID" value="Nwi_3075"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3075"/>
    <dbReference type="eggNOG" id="COG0594">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117179_6_0_5"/>
    <dbReference type="OMA" id="GFTCSKK"/>
    <dbReference type="OrthoDB" id="2014742at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00227">
        <property type="entry name" value="RNase_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000100">
        <property type="entry name" value="RNase_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020539">
        <property type="entry name" value="RNase_P_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33992">
        <property type="entry name" value="PTHR33992"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00825">
        <property type="entry name" value="Ribonuclease_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00188">
        <property type="entry name" value="rnpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00648">
        <property type="entry name" value="RIBONUCLEASE_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA06323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="111" mass="12844" checksum="25E56ACFA4198F85" modified="2005-10-11" version="1">MDRLRQRADFLAAAHGPRVSTPAFVVQNRRRSDDGAIRIGFTVTKKNGTATERNRIRRRLRELVKRVDAVSMRPHSDYVIIGRRAALNRRFAVMLDDLRSALQRLDRRHPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNC2</accession>
    <name>Q3SNC2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphoserine transaminase</fullName>
            <ecNumber evidence="6">2.6.1.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_2969</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-oxoglutarate + 4-(phosphooxy)-L-threonine = (R)-3-hydroxy-2-oxo-4-phosphooxybutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:16573"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58452"/>
            <dbReference type="ChEBI" id="CHEBI:58538"/>
            <dbReference type="EC" id="2.6.1.52"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2-oxoglutarate + O-phospho-L-serine = 3-phosphooxypyruvate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:14329"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:18110"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57524"/>
            <dbReference type="EC" id="2.6.1.52"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.52" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316141.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2969"/>
    <dbReference type="EnsemblBacteria" id="ABA06219">
        <property type="protein sequence ID" value="ABA06219"/>
        <property type="gene ID" value="Nwi_2969"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2969"/>
    <dbReference type="eggNOG" id="COG1932">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040283_0_0_5"/>
    <dbReference type="OMA" id="TAFWDIA"/>
    <dbReference type="OrthoDB" id="996960at2"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00197"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004648">
        <property type="term" value="F:O-phospho-L-serine:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022278">
        <property type="entry name" value="Pser_aminoTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006271">
        <property type="entry name" value="Pser_aminoTfrase_methanosarc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000525">
        <property type="entry name" value="SerC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01365">
        <property type="entry name" value="serC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0718">Serine biosynthesis</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA06219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="390" mass="41927" checksum="AEA0A45371E33C13" modified="2005-10-11" version="1">MTVAKPASRPDVPHFSSGPCAKRPGWTPQNLKDAALGRSHRAKIGKARLKLAIDLTREVLEIPADYRIGIVPASDTGAVEMALWSLLGARPVTAIAWESFGEGWITDVIKQLKLKDVTKLTAGYGEIPDLAKADPSSDIVFTWNGTTSGVRVPNADWISAAREGLTICDATSAAFAQPLDWAKLDVVTFSWQKALGGEAAHGMLILSPRAVARLESYTPPWPMPKIFRMTKGGKLIDGIFAGETINTPSMLCVEDYLDALNWARSVGGLKGMIARADANTRVLSDWVAKTPWIDFLAADPAIRSNTSVCLKVVDPAITSLSADAQADFAKKLVAAIEKEGAGYDFGYYRDAPPGLRIWCGATVEASNVALLTEWIDWAFAETKASLAKAA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNC6</accession>
    <name>PURA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Adenylosuccinate synthetase</fullName>
            <shortName evidence="1">AMPSase</shortName>
            <shortName evidence="1">AdSS</shortName>
            <ecNumber evidence="1">6.3.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">IMP--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purA</name>
        <name type="ordered locus">Nwi_2965</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + IMP + L-aspartate = GDP + 2 H(+) + N(6)-(1,2-dicarboxyethyl)-AMP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="6.3.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the adenylosuccinate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.4" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316137.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SNC6"/>
    <dbReference type="SMR" id="Q3SNC6"/>
    <dbReference type="STRING" id="323098.Nwi_2965"/>
    <dbReference type="EnsemblBacteria" id="ABA06215">
        <property type="protein sequence ID" value="ABA06215"/>
        <property type="gene ID" value="Nwi_2965"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2965"/>
    <dbReference type="eggNOG" id="COG0104">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029848_0_0_5"/>
    <dbReference type="OMA" id="FHHAKPI"/>
    <dbReference type="OrthoDB" id="232152at2"/>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00335"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004019">
        <property type="term" value="F:adenylosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03108">
        <property type="entry name" value="AdSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.170.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00011">
        <property type="entry name" value="Adenylosucc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018220">
        <property type="entry name" value="Adenylosuccin_syn_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033128">
        <property type="entry name" value="Adenylosuccin_syn_Lys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042109">
        <property type="entry name" value="Adenylosuccinate_synth_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042110">
        <property type="entry name" value="Adenylosuccinate_synth_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042111">
        <property type="entry name" value="Adenylosuccinate_synth_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001114">
        <property type="entry name" value="Adenylosuccinate_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846">
        <property type="entry name" value="PTHR11846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00709">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00788">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00184">
        <property type="entry name" value="purA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01266">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00513">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000224297" description="Adenylosuccinate synthetase">
        <location>
            <begin position="1"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="41"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="12"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="13"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="38"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="40"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="299"/>
            <end position="305"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="331"/>
            <end position="333"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="413"/>
            <end position="415"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00011"/>
        </source>
    </evidence>
    <sequence length="430" mass="46636" checksum="362FFC27F25034DC" modified="2005-10-11" version="1">MTNVVVVGAQWGDEGKGKIVDWLSEQADIVVRFQGGHNAGHTLVIDGNTYKLALLPSGVVRSSKLSVIGNGVVFDPQAFVNEVEKLKEQGVKVGPDNLRVAENVTLILPLHRELDSLRENASAATAIGTTQRGIGPAYEDKVGRRAIRLMDLADPQTLPHKIERLLAHHNALRRGHGIAEVDSGALLRDLAAIAPRVLPYADSVWNLLDHKRREGKRILFEGAQGALLDVDHGTYPYVTSSNTVAAQAATGTGMGPSALGYVLGICKAYTTRVGQGPFPTELNDEIGELIGQRGKEFGVNTGRKRRCGWFDAMLVRQTVRTSGIHGLALTKLDILDGFDTIQVCTGYRLDGKEIDHLPAGEGAQARIEPVYETVEGWKEPTANARSWADLPAQAIKYVRRIEELVGCPVALLSTSPEREDTILVQNPFEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNF2</accession>
    <name>Q3SNF2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Heat shock protein Hsp20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2939</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2939"/>
    <dbReference type="EnsemblBacteria" id="ABA06189">
        <property type="protein sequence ID" value="ABA06189"/>
        <property type="gene ID" value="Nwi_2939"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2939"/>
    <dbReference type="eggNOG" id="COG0071">
        <property type="taxonomic scope" value="Bacteria"/>
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    <dbReference type="HOGENOM" id="CLU_046737_4_1_5"/>
    <dbReference type="OMA" id="EMKPHRI"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06470">
        <property type="entry name" value="ACD_IbpA-B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR037913">
        <property type="entry name" value="ACD_IbpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="4">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA06189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="108" mass="11759" checksum="E55FD850029C559D" modified="2005-10-11" version="1">MAVAGLTEADLAITQEQNMLLVAGQKSGQDEASGHYLHRGIAARAFQRRFELADHVKVVAANLVNGLLTIDLKRELPEEMKPRRIAITAGEALTKAETKQVQPEKQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNH4</accession>
    <name>Q3SNH4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Integrase, catalytic region</fullName>
            <ecNumber evidence="2">2.7.7.49</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2917</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316097.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2917"/>
    <dbReference type="EnsemblBacteria" id="ABA06167">
        <property type="protein sequence ID" value="ABA06167"/>
        <property type="gene ID" value="Nwi_2917"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2917"/>
    <dbReference type="eggNOG" id="COG2801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027402_15_1_5"/>
    <dbReference type="OMA" id="AHKWIRR"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13683">
        <property type="entry name" value="rve_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="127"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="321" mass="36747" checksum="BA241ADF4383653F" modified="2005-10-11" version="1">MGQVLHGSATTTEAIRRAIQNSEESLRALSKRYGINQKTVAKWKKRSSVADLPTGPKEPHSSVLSLEDEAIIVAFRKHTLLPLDDCLYALQPTIPHLTRSSLHRCLQRHGISRLPDVEGDKAPRRKFKCYPIGYFHIDIAQIQTAEGKLQLFVAIDRTSKFAFARLEESATVHTAAAFLKALIEAVPYEIHTVLTDNGTQFCDLPSRRNGPTARLRLHMFDRVCFGNGIEHRLTKPNHPWTNGQVERMNRTIKDATVKRFHYDNHDQLRRHLQDFIDAYNFGRRLKTLNGLTPYELICKQWTSEPDRFIIDPIHQMPGLNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNN9</accession>
    <name>Q3SNN9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase, family 28</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2852</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316040.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2852"/>
    <dbReference type="CAZy" id="GT1">
        <property type="family name" value="Glycosyltransferase Family 1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06102">
        <property type="protein sequence ID" value="ABA06102"/>
        <property type="gene ID" value="Nwi_2852"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2852"/>
    <dbReference type="eggNOG" id="COG1819">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000537_8_0_5"/>
    <dbReference type="OMA" id="ICPFFGD"/>
    <dbReference type="OrthoDB" id="1485440at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030259">
        <property type="term" value="P:lipid glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033072">
        <property type="term" value="P:vancomycin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004276">
        <property type="entry name" value="GlycoTrans_28_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03033">
        <property type="entry name" value="Glyco_transf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_transf_28" evidence="1">
        <location>
            <begin position="5"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="414" mass="43453" checksum="D125D477181C031C" modified="2005-10-11" version="1">MRFSIHTLGTRGDTQPYLALSRGLRAHGHGVLIVAPAQFADMAAAEGVAFAPLPAEFLAVLDSPEAKQVIGSAGTGFGAGFKLLKFYREISRRLLDAEWKAARDFAPDAILFHPKALGAPHIAAKLGVPLFLASPLPGFTPTSAFPTPVLPFPSLGPLNRASHALMIHGGSLLFGKTIRAWRVDALGLSARGKAAPLAGTLYGYSPYVLPKPHDWDADVAVTGYWFLDTPNWSPDGELAAFLAAGEPPIYVGFGSMPGVDPQRMASVVVAGLKRADKRGVLATAGGALGQIEPSQHIHVIVGAPHDRLFPLMHATLHHGGAGTTGAALRAGKPMAICPFFGDQPFWARRVVALGVGPKPLDKKAMTADDLAAAFLAMDNHDMRARAADLGVAIRAENGVDAAVRFIVEGACCGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNW4</accession>
    <name>Q3SNW4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Signal transduction histidine kinase</fullName>
            <ecNumber evidence="3">2.7.13.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2774</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.13.3" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315972.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2774"/>
    <dbReference type="EnsemblBacteria" id="ABA06027">
        <property type="protein sequence ID" value="ABA06027"/>
        <property type="gene ID" value="Nwi_2774"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2774"/>
    <dbReference type="eggNOG" id="COG3614">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3920">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_494215_0_0_5"/>
    <dbReference type="OMA" id="FRWEEFN"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004673">
        <property type="term" value="F:protein histidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006189">
        <property type="entry name" value="CHASE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011102">
        <property type="entry name" value="Sig_transdc_His_kinase_HWE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03924">
        <property type="entry name" value="CHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07536">
        <property type="entry name" value="HWE_HK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01079">
        <property type="entry name" value="CHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00911">
        <property type="entry name" value="HWE_HK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50839">
        <property type="entry name" value="CHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="298"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="CHASE" evidence="2">
        <location>
            <begin position="142"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="552" mass="60588" checksum="1FA54A247DF9384E" modified="2005-10-11" version="1">MVRLGFVIGFVAVIGIFLSGLAAWRAHDQELAIDRIALSRAIDVHASLVQERLIQRELLARVASGLFRSPSAMQANMLHPLRASIYAFETDFVLAAWVARLESGEFPQARDALANAGFPNPQIRSFDDASRDLSSLDKPIDVIMDVEPQNAQTSAFPGRSLDQHPILGPMFARAMQTKTSVVSDPLNLMDRDGPIGLVIATPVFQENSGTAAGFITFSYKLASLMLTNDELSLFSVVLKDPRNPALELVADDSGDVTSRPGQTADPAPPVFRTVTFGGRDWSLGYYAKTNASHRAQKLAAVVAAIGLALTSIICGLFGYVAYNNLRLSREIQVRIGFERRLTAVIDELNHRVKNILAVIQSIVTRTLRHGSDIEVARELLIGRIHAMSNVVSLLSESQWQGVKLRGLFEARAIPHVDRIAIHGPDITVSARAAQSLSLLFFELASHSDEGLSLVGKHPHIVVNWEVEGEGEEALFRFRWEEFNTSAATRREDSDFGIILLDRVAPEALGGISKRYFTEVSYVYELAAPIDTVVDLTERDRTMQISTPLKPGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNY3</accession>
    <name>Q3SNY3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">3-hydroxybutyrate dehydrogenase</fullName>
            <ecNumber evidence="1">1.1.1.30</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2755</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.30" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315953.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2755"/>
    <dbReference type="EnsemblBacteria" id="ABA06008">
        <property type="protein sequence ID" value="ABA06008"/>
        <property type="gene ID" value="Nwi_2755"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2755"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_0_5"/>
    <dbReference type="OMA" id="QHGKIDN"/>
    <dbReference type="OrthoDB" id="1094766at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003858">
        <property type="term" value="F:3-hydroxybutyrate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011294">
        <property type="entry name" value="3-OHbutyrate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01963">
        <property type="entry name" value="PHB_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="262" mass="27353" checksum="D4EAA4CCD173258E" modified="2005-10-11" version="1">MTKLKGKTAVVTGSTSGIGLAYARAFAGAGANVVINGLGAEADIEKARSGLEAEFEVKAVYSPADMTKPAEIAGMIELGETTFGSVDILVNNAGIQFVSPVESFPVEKWDAIIAINLSSAFHAIRAAVPGMKKRGWGRIITTASAHSTVASPFKSAYVAAKHGIAGLTKAVALELAPHRITCNCISPGYVWTSLVEKQIPDTMKSRGMTREQVVNDVLLAAQPTKQFVTVEQVAALALYLCSDDAAQITGSNLSIDGGWTAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNY4</accession>
    <name>Q3SNY4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Polysaccharide biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_2754</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2754"/>
    <dbReference type="EnsemblBacteria" id="ABA06007">
        <property type="protein sequence ID" value="ABA06007"/>
        <property type="gene ID" value="Nwi_2754"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2754"/>
    <dbReference type="eggNOG" id="COG2244">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022017_5_4_5"/>
    <dbReference type="OMA" id="GLMPQFI"/>
    <dbReference type="OrthoDB" id="564091at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029303">
        <property type="entry name" value="Polysacc_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002797">
        <property type="entry name" value="Polysacc_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01943">
        <property type="entry name" value="Polysacc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14667">
        <property type="entry name" value="Polysacc_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="323"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="354"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="397"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="421"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="Polysacc_synt_C" evidence="7">
        <location>
            <begin position="366"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA06007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="460" mass="50053" checksum="817093098FEAF9D7" modified="2005-10-11" version="1">MAITDAPSAPAKRLPMLIGRLRAILGGSSEASLTRRLAGTIFIIRVISAGIAYVAQILLARWMGGSDYGVYVYVWTWVLLLGSMMDFGISASAQRIIPEYHASGDNARLRGFLSGSRWMTFIVASLISASLAALVTALSPWIDAATVLPLYIGCLTLPAFVVANTQDGIARSHDWMRLGLMPQFIVRQSLIIGFTAGAFVLGFHLSATVAMLASVAAVWIAMIGQMIVLNRRLRTIVTPGPKVHDFRGWLAVSLPILLVESFYLLLSYTDVLVLQQFRSSEEVGVYFAVVKTLALVSFIHYAMSATTAHRFTEYHAAGDRERLAAYLAYAIKWTFWPSLAATLLLLAFGKPMLWLFGPQFVTGYSIMFVAAIGLVVRSAIGPVERLLNMLGHQHACAAAYALAFGMNVALCFAFVPRYGGHGAAAATSISLTFETVLLFWIVRRKLGFHVLAFGKGRAST</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP25</accession>
    <name>Q3SP25_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cell division coordinator CpoB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cpoB</name>
        <name evidence="3" type="ordered locus">Nwi_2713</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mediates coordination of peptidoglycan synthesis and outer membrane constriction during cell division.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CpoB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2713"/>
    <dbReference type="EnsemblBacteria" id="ABA05966">
        <property type="protein sequence ID" value="ABA05966"/>
        <property type="gene ID" value="Nwi_2713"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2713"/>
    <dbReference type="eggNOG" id="COG1729">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044315_0_1_5"/>
    <dbReference type="OMA" id="IQSHPRD"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030288">
        <property type="term" value="C:outer membrane-bounded periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02066">
        <property type="entry name" value="CpoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034706">
        <property type="entry name" value="CpoB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014162">
        <property type="entry name" value="CpoB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13174">
        <property type="entry name" value="TPR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02795">
        <property type="entry name" value="tol_pal_ybgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="1" id="KW-0574">Periplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="31"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="44"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="305" mass="32400" checksum="700021E30A419678" modified="2005-10-11" version="1">MLMLSSHASAQYDIDPELRIQQLEQQLRTLTGQNEELQHRNRMLEQQLRQLQPGQASSGVAATAPASPGPQASSSQQPGYDQRGGQPPLVQEQAATAPAQGRRGDAFDPSQNPGAPGAPRALGGGQMPIPTAAVGSPGGREAGAPLDLANVGPRSSVSHPAAGSAPLSTGGLTTLPPSATPRDQFDLGIGYMQRKDYALAEETMRSFTEKYPSDTLTGDAQYWLGESLFQRQKYREAAEAFLGVTTKYDSSAKVADALLRLGQSLAALKEKEAACAAFGEVTRKYPRASAGVKQAVDREQRRVKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP26</accession>
    <name>Q3SP26_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2712</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315911.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2712"/>
    <dbReference type="EnsemblBacteria" id="ABA05965">
        <property type="protein sequence ID" value="ABA05965"/>
        <property type="gene ID" value="Nwi_2712"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2712"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2667396_0_0_5"/>
    <dbReference type="OrthoDB" id="1900922at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="75" mass="8269" checksum="F9B2B27170D21288" modified="2005-10-11" version="1">MTIHLQAARKTQRRSGRGAVNVFATEVDALHDELRSRGVAIVHPPGDRPYGMRDFEIYNLDGNRLTFGMGIGGSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP95</accession>
    <name>Q3SP95_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glucose-6-phosphate 1-dehydrogenase</fullName>
            <shortName evidence="5">G6PD</shortName>
            <ecNumber evidence="5">1.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">zwf</name>
        <name evidence="9" type="ordered locus">Nwi_2643</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:15841"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="1.1.1.49"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the glucose-6-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.49" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315846.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2643"/>
    <dbReference type="EnsemblBacteria" id="ABA05896">
        <property type="protein sequence ID" value="ABA05896"/>
        <property type="gene ID" value="Nwi_2643"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2643"/>
    <dbReference type="eggNOG" id="COG0364">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013524_5_1_5"/>
    <dbReference type="OMA" id="NIENGWR"/>
    <dbReference type="OrthoDB" id="1153269at2"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00408"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004345">
        <property type="term" value="F:glucose-6-phosphate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00966">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001282">
        <property type="entry name" value="G6P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019796">
        <property type="entry name" value="G6P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022675">
        <property type="entry name" value="G6P_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022674">
        <property type="entry name" value="G6P_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429">
        <property type="entry name" value="PTHR23429"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02781">
        <property type="entry name" value="G6PD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00479">
        <property type="entry name" value="G6PD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000110">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00079">
        <property type="entry name" value="G6PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00871">
        <property type="entry name" value="zwf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00069">
        <property type="entry name" value="G6P_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1 5" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="2 5" id="KW-0521">NADP</keyword>
    <keyword evidence="3 5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G6PD_N" evidence="7">
        <location>
            <begin position="17"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="G6PD_C" evidence="8">
        <location>
            <begin position="202"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="253"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="353"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="504" mass="55944" checksum="D7DF529E804AF79F" modified="2005-10-11" version="1">MTVASNIPRKPEPCCFVIFGATGDLTHRLIIPALYNLAESGLLPEPFCIVGVVRKQMSDQDLRQSLMEGLKRHATRPVKIDIAEKVLNCLTCIEADPSDPSSFDALNGVCEGLATKTGTCGNRLFYLAVPPTAFAPIAEQLARVGLLRETSGTWRRLVVEKPFGTDLASARSLNARLLKFANEHQIYRIDHYLGKETVQNILVLRFANGMFEPIWNRNHIDHIQITVEEKLSVGHRGSFYDATGALRDMVPNHLFQLLSLVTMEPPTRFEAHSVRSQKAEVLAAIQTQTPEEALRNSVRGQYHAGQVGGEAVVDYRSAPNVKPDSTTETYAALKLTIDNWRWAGVPFYLRTGKALGLKRTEVAIKFKQAPFAMFRCTPVDQLSHNYLVIGIEPAEGITMQFNTKVPGPSISIDGVEMKFRYKDYFHAAPSTGYETLIYDCMTGDNILFQRADGVEAGWQAVQPFLDAWKKAGADGLVGYEAGSEGPREADDLLARDGNQWRELT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP99</accession>
    <name>Q3SP99_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutathione peroxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_2639</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the glutathione peroxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315842.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2639"/>
    <dbReference type="PeroxiBase" id="5782">
        <property type="entry name" value="NwGPx"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05892">
        <property type="protein sequence ID" value="ABA05892"/>
        <property type="gene ID" value="Nwi_2639"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2639"/>
    <dbReference type="eggNOG" id="COG0386">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029507_2_2_5"/>
    <dbReference type="OMA" id="FPMMSKI"/>
    <dbReference type="OrthoDB" id="1635499at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004602">
        <property type="term" value="F:glutathione peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00340">
        <property type="entry name" value="GSH_Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000889">
        <property type="entry name" value="Glutathione_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029759">
        <property type="entry name" value="GPX_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029760">
        <property type="entry name" value="GPX_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592">
        <property type="entry name" value="PTHR11592"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00255">
        <property type="entry name" value="GSHPx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000303">
        <property type="entry name" value="Glutathion_perox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01011">
        <property type="entry name" value="GLUTPROXDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00460">
        <property type="entry name" value="GLUTATHIONE_PEROXID_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00763">
        <property type="entry name" value="GLUTATHIONE_PEROXID_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51355">
        <property type="entry name" value="GLUTATHIONE_PEROXID_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 5 6" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="4">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000303-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="158" mass="17594" checksum="B8C48ECFF14E3A44" modified="2005-10-11" version="1">MASVFDFSANALDGQPCELKQFEGRVLLIVNTASACGFTPQYKDLEELHRTMSPRGFSVLGFPCNQFGKQEPGSAADIQQFCASKYDVTFPMFEKINVNGADAHPLFRHLKSEKPGLLGESIKWNFTKFLVDRQGRVAARHAPTTNPKKLTEEIEALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPC0</accession>
    <name>Q3SPC0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA (cytidine(34)-2'-O)-methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.207</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmL</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">trmL</name>
        <name evidence="7" type="ordered locus">Nwi_2618</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S-adenosyl-L-methionine to the 2'-OH of the wobble nucleotide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>5-carboxymethylaminomethyluridine(34) in tRNA(Leu) + S-adenosyl-L-methionine = 5-carboxymethylaminomethyl-2'-O-methyluridine(34) in tRNA(Leu) + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:43088"/>
            <dbReference type="Rhea" id="RHEA-COMP:10333"/>
            <dbReference type="Rhea" id="RHEA-COMP:10334"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74508"/>
            <dbReference type="ChEBI" id="CHEBI:74511"/>
            <dbReference type="EC" id="2.1.1.207"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>cytidine(34) in tRNA + S-adenosyl-L-methionine = 2'-O-methylcytidine(34) in tRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:43084"/>
            <dbReference type="Rhea" id="RHEA-COMP:10331"/>
            <dbReference type="Rhea" id="RHEA-COMP:10332"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.207"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. TrmL subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.207" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315821.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2618"/>
    <dbReference type="EnsemblBacteria" id="ABA05871">
        <property type="protein sequence ID" value="ABA05871"/>
        <property type="gene ID" value="Nwi_2618"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2618"/>
    <dbReference type="eggNOG" id="COG0219">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110125_2_0_5"/>
    <dbReference type="OMA" id="AGLDYWH"/>
    <dbReference type="OrthoDB" id="1578095at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01885">
        <property type="entry name" value="tRNA_methyltr_TrmL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016914">
        <property type="entry name" value="tRNA_cyt/urid_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42971">
        <property type="entry name" value="PTHR42971"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029256">
        <property type="entry name" value="SpoU_TrmH_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 4 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SpoU_methylase" evidence="6">
        <location>
            <begin position="2"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4 5">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 5">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 5">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR029256-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="156" mass="17102" checksum="CA5A5CA649D0D3C0" modified="2005-10-11" version="1">MRIALFQPDIPQNTGTILRLCACLGVEAHIIEPAGFPVSDRHFRRAGMDYLDQVTIVRHDSWLKFEQWRAGDGGRLVLFTTRGALPYLDHRYAATDILLFGRESAGVPDAVAAAADARLVIPLRPALRSLNVAMAAAMAVGEALRQTEPALTERIP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-16" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPE5</accession>
    <name>ISPE_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4-diphosphocytidyl-2-C-methyl-D-erythritol kinase</fullName>
            <shortName evidence="1">CMK</shortName>
            <ecNumber evidence="1">2.7.1.148</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ispE</name>
        <name type="ordered locus">Nwi_2593</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4-CDP-2-C-methyl-D-erythritol + ATP = 4-CDP-2-C-methyl-D-erythritol 2-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57823"/>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.148"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GHMP kinase family. IspE subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.148" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315796.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SPE5"/>
    <dbReference type="SMR" id="Q3SPE5"/>
    <dbReference type="STRING" id="323098.Nwi_2593"/>
    <dbReference type="EnsemblBacteria" id="ABA05846">
        <property type="protein sequence ID" value="ABA05846"/>
        <property type="gene ID" value="Nwi_2593"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2593"/>
    <dbReference type="eggNOG" id="COG1947">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053057_1_0_5"/>
    <dbReference type="OMA" id="RWPSPAK"/>
    <dbReference type="OrthoDB" id="1938933at2"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00094"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050515">
        <property type="term" value="F:4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00061">
        <property type="entry name" value="IspE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004424">
        <property type="entry name" value="IspE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43527:SF2">
        <property type="entry name" value="PTHR43527:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010376">
        <property type="entry name" value="IspE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="SSF55060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00154">
        <property type="entry name" value="ispE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000235106" description="4-diphosphocytidyl-2-C-methyl-D-erythritol kinase">
        <location>
            <begin position="1"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="23"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00061"/>
        </source>
    </evidence>
    <sequence length="308" mass="31932" checksum="0B9E1D5C51DB7EB2" modified="2005-10-11" version="1">MIVTGATDLETAAPALSETARAKVNLTLRVVGRRADGFHDLESVVAFADCVDRLTLTQGAELSLVAAGPRAQECGQTADNLVLTAARLLGERIPDLKAGAFALDKYLPVAAGIGGGSADAAAALRLLARANGLSIDDPRLIETARLTGADVPVCVRSESCVMTGVGESLLPLDVPAMPAVLVNPRVPVATKDVFAALGLRKGQLNVGVNDLIEAIVWPEAGAPVDDWLAMLSDGANDLEAPAIRVQPVIGEVLARLRSTGARLSRMSGSGATCFAIFDDEAAAHHAAQTIRLDRPQWWVHVGTLGGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPE6</accession>
    <name>Q3SPE6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Polyprenyl synthetase</fullName>
            <ecNumber evidence="3">2.5.1.29</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2592</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.29" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315795.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2592"/>
    <dbReference type="EnsemblBacteria" id="ABA05845">
        <property type="protein sequence ID" value="ABA05845"/>
        <property type="gene ID" value="Nwi_2592"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2592"/>
    <dbReference type="eggNOG" id="COG0142">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014015_2_0_5"/>
    <dbReference type="OMA" id="GKQMRPM"/>
    <dbReference type="OrthoDB" id="1861068at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004311">
        <property type="term" value="F:farnesyltranstransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="SSF48576"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="336" mass="35908" checksum="FFE63634EE104187" modified="2005-10-11" version="1">MAVIVPFESQPAGSIDQLVELVAADMERVNATILSRTGSDVTMIPEVANHLISSGGKRLRPMLTLAMANLAGYAGEGHVKLAASVEFMHTATLLHDDVVDESELRRGRLSARMVWGNEASVLVGDFLLGQAFRMMVEVGSLRALDILSSAAATIAEGEVMQLAAAKNTATTEDEYLAVIRGKTAELFAAACEVGPVLGGRPKAEQTAAASFGMNLGIAFQLVDDVLDYGGKSAKLGKNVGDDFREGKITLPVVLAFRRGNDVEREFWVRALEQGKIGDNDLNHAIGLMTKHRALEDTLNRAHHYGAMAVDALALFPPSPMKGALEQVVAFCLARSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPF9</accession>
    <name>Q3SPF9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycolate oxidase iron-sulfur subunit</fullName>
            <ecNumber evidence="4">1.1.99.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_2579</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of a complex that catalyzes the oxidation of glycolate to glyoxylate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(R)-lactate + A = AH2 + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:15089"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:16004"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>A + glycolate = AH2 + glyoxylate</text>
            <dbReference type="Rhea" id="RHEA:21264"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29805"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="EC" id="1.1.99.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="4">Binds 2 [4Fe-4S] clusters.</text>
    </comment>
    <dbReference type="EC" id="1.1.99.14" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315783.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2579"/>
    <dbReference type="EnsemblBacteria" id="ABA05832">
        <property type="protein sequence ID" value="ABA05832"/>
        <property type="gene ID" value="Nwi_2579"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2579"/>
    <dbReference type="eggNOG" id="COG0247">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023081_0_0_5"/>
    <dbReference type="OMA" id="VYQDACH"/>
    <dbReference type="OrthoDB" id="1951488at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012257">
        <property type="entry name" value="Glc_ox_4Fe-4S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32479:SF17">
        <property type="entry name" value="PTHR32479:SF17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000139">
        <property type="entry name" value="Glc_ox_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="2 4" id="KW-0408">Iron</keyword>
    <keyword evidence="3 4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="14"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="67"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="437" mass="47168" checksum="60254D4D94F6C2A5" modified="2005-10-11" version="1">MRTEFTPAQLSDPDIAEADKILRKCVHCGFCTATCPTYVLLGDELDSPRGRIYLIKQMLEHDEPPTAEVVKHIDRCLSCLACMTTCPSGVNYMHLVDQARVRIEKDYARPVSERLLRAVLAFVLPRPKVFRASLILARLARPFAALLPGAKGSVTPTLPRRIKAMLALAPASLPAPGAAAGRVFPATGKRRGRVALLQGCAQQVLAPRINEAAIRLLTRHGVEVVLVADEQCCGALAYHMGDDRDALARARANIKAWTREAERGGLDAILITTSGCGTVIKDYGYLLREDRDFAGPAARVSSLAKDITEYIASLDLPASGSRSELVIAYHSACSLQHGQKITQLPKDLLFKNGFVVKDIPESHLCCGSAGTYNILQPDIADRLRDRKVANIASVKPDMIAAGNIGCMIQIAGGTSVPVVHTIELLDWATGGPRPGLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPR7</accession>
    <name>Q3SPR7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Oligopeptidase B, Serine peptidase, MEROPS family S09A</fullName>
            <ecNumber evidence="3">3.4.21.83</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2471</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.4.21.83" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315679.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2471"/>
    <dbReference type="ESTHER" id="nitwn-q3spr7">
        <property type="family name" value="S9N_PREPL_Peptidase_S9"/>
    </dbReference>
    <dbReference type="MEROPS" id="S09.010"/>
    <dbReference type="EnsemblBacteria" id="ABA05724">
        <property type="protein sequence ID" value="ABA05724"/>
        <property type="gene ID" value="Nwi_2471"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2471"/>
    <dbReference type="eggNOG" id="COG1770">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011290_0_1_5"/>
    <dbReference type="OMA" id="NMGAGHG"/>
    <dbReference type="OrthoDB" id="154149at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023302">
        <property type="entry name" value="Pept_S9A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002470">
        <property type="entry name" value="Peptidase_S9A"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02897">
        <property type="entry name" value="Peptidase_S9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00862">
        <property type="entry name" value="PROLIGOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_S9_N" evidence="2">
        <location>
            <begin position="8"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S9" evidence="1">
        <location>
            <begin position="476"/>
            <end position="689"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="695" mass="77207" checksum="F30AFF8B572EAE99" modified="2005-10-11" version="1">MTSTPTPPKADRRSHTYSHHGITLTDDYAWLKDANWQQVLREPSLLEPDIRAYLEAETAYSNAVLAQTEPLQRQLVAEMRGRIKEDDSTVPSPDGPFAYFTKYREGGQHRMIGRQPRDGGDARFLIDGDALAENKKFFQLGEADHSPDHRLEAWSCDDRGSEYFTIRIRDWDSGADLADVIEETDGGVVWSADSRAFYYVKLDDNHRPMQVYRHQIGTVQADDTLIYEEQDIGWFTHIHESASGRFCVIAGGDHETSEQRLIDLAAPQAVPSLIAPREDGVQYSVADRGDELFILTNADDAIDFKIMTAPLSSPDRANWRDLIPYRPGTYVIDFELYSGHMARLERTNALPSIIIRDLATGDEHAIAFDEAAYSLDTHGGYEFDTTTLRFSYSSMTTPSEVFDYDMASRSRTLRKRQEIPSGHDPANYVTTRIMAKADDGAEVPVSILHRKDFVLDGRAPLLLYGYGSYGHSMPASFSANRLSLVDRGFAYAIAHIRGGADKGWGWYLDGKREKKTNSFDDFAACARALIAANYTSAKRIVGHGGSAGGMLMGAVANRAGELFAGIVAEVPFVDVLNTMLDDTLPLTPPEWPEWGNPIVSAEDFRTILSYSPYENVAAKDYPAILALGGLTDPRVTYWEPAKWIARLRATMTSGGPVLLRTNMGAGHGGASGRFNRLDEVALAYAFALWAVEYDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPV5</accession>
    <name>Q3SPV5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2432</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315644.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05686">
        <property type="protein sequence ID" value="ABA05686"/>
        <property type="gene ID" value="Nwi_2432"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2432"/>
    <dbReference type="eggNOG" id="ENOG50336US">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_171129_0_0_5"/>
    <dbReference type="OMA" id="EIRTFDA"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="110" mass="11681" checksum="9261F45F072F8D86" modified="2005-10-11" version="1">MTEPLNPEPTPEQAALFARVRRMMLIAGLTTALAVGAVLVAIGYRFFRVEGSRPAADVTATLPKDARITATAVAGDLLVVTLDLGGSVQIRTFNLRTLQSAGTLNFAREP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPX3</accession>
    <name>Q3SPX3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2414</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315626.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2414"/>
    <dbReference type="EnsemblBacteria" id="ABA05668">
        <property type="protein sequence ID" value="ABA05668"/>
        <property type="gene ID" value="Nwi_2414"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2414"/>
    <dbReference type="eggNOG" id="ENOG50302JN">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2452650_0_0_5"/>
    <dbReference type="OMA" id="VRCKQSA"/>
    <dbReference type="OrthoDB" id="2055514at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229112" evidence="1">
        <location>
            <begin position="26"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="89" mass="9658" checksum="6C7552DA9900DF2A" modified="2005-10-11" version="1">MMTFRIPLAGVLAAAVVSVAISASAAAGSRHHKHYHTQHSPETDIYLHNYGPAVTPDQPFAYYDGPVRVRCKQSAAAYRGQDGRPHPCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPZ8</accession>
    <name>Q3SPZ8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2389</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2389"/>
    <dbReference type="EnsemblBacteria" id="ABA05643">
        <property type="protein sequence ID" value="ABA05643"/>
        <property type="gene ID" value="Nwi_2389"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2389"/>
    <dbReference type="eggNOG" id="COG1045">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107086_0_0_5"/>
    <dbReference type="OMA" id="ASCLTHI"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="232" mass="25872" checksum="7A73C91254FCE3F2" modified="2005-10-11" version="1">MSGRAPAAGIDVQLSLDRSDLTAYVARQLESFFPDGFPLDDLPAFVDRALERLEYCFSRVRLKGYFDGEQSRFSHRHSDQYAVFLYYLSNSTFREKPGHPIAEKAYALNKALHALDAFYEVELPDIFAVQHPVGTVLGRATYSDYFMCYQNCTVGANLDSDYPEFGQGIVIYGGGRVIGRTSVGDNTFVSTGAIVMDAGALHGDCVLHGIFPRTQRSPTRRNVIRDIFGWAD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ28</accession>
    <name>Y2359_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0391 membrane protein Nwi_2359</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">Nwi_2359</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0391 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SQ28"/>
    <dbReference type="STRING" id="323098.Nwi_2359"/>
    <dbReference type="EnsemblBacteria" id="ABA05613">
        <property type="protein sequence ID" value="ABA05613"/>
        <property type="gene ID" value="Nwi_2359"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2359"/>
    <dbReference type="eggNOG" id="COG5487">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_187346_2_1_5"/>
    <dbReference type="OMA" id="EIAWILF"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01361">
        <property type="entry name" value="UPF0391"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009760">
        <property type="entry name" value="DUF1328"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07043">
        <property type="entry name" value="DUF1328"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036466">
        <property type="entry name" value="UCP036466"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000256751" description="UPF0391 membrane protein Nwi_2359">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="4"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01361"/>
        </source>
    </evidence>
    <sequence length="57" mass="6096" checksum="816BB2D17DBE8E0D" modified="2005-10-11" version="1">MLNWVVTFLVIALIAGVLGFGGIAGASFEIAKIIFFIALILFVISAVVGFLRGRNRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ38</accession>
    <name>Q3SQ38_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein HflK</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_2349</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">HflC and HflK could encode or regulate a protease.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">HflC and HflK may interact to form a multimeric complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the band 7/mec-2 family. HflK subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315567.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2349"/>
    <dbReference type="EnsemblBacteria" id="ABA05603">
        <property type="protein sequence ID" value="ABA05603"/>
        <property type="gene ID" value="Nwi_2349"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2349"/>
    <dbReference type="eggNOG" id="COG0330">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039173_0_1_5"/>
    <dbReference type="OMA" id="WNEPGGN"/>
    <dbReference type="OrthoDB" id="577287at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03404">
        <property type="entry name" value="SPFH_HflK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010201">
        <property type="entry name" value="HflK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020980">
        <property type="entry name" value="Membrane_HflK_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43327:SF2">
        <property type="entry name" value="PTHR43327:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12221">
        <property type="entry name" value="HflK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="SSF117892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01933">
        <property type="entry name" value="hflK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="47"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="PHB" evidence="8">
        <location>
            <begin position="72"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="363"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="247"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="16"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="382" mass="41970" checksum="414B0FE5F1D04F46" modified="2005-10-11" version="1">MPWKNQTGGPWGPGPKGPWGAGPQPTGPKPPDLEDLLRRAQERIRQLLPGGHLSGMGILLILIGAVAIWGMSGFFRVQSEELGVVLRFGKHVRTVQPGLNYHLPYPIETVLLPKALRVSTLNIGLTLVQDSARSTSTMRDVPEESLMLTGDENIVDVDFTVLWRIKPDGVGDFLFNIQNPEGTVKAVAESAMREWVGRSDIQPILTSERTKIEASVHELMQKTLDQYGAGVLIQQVQMQKVDPPAQVIDSFRDVQAARADLERLQNEAQTYANRVVPDSRGRAAQIVQNAQGYKEQAIAEAKGQSSRFLQVYQAYKEAPDVTRERIYLETMEHVLGDADKLIYDPGSSSSGGIVPYLPLSELTSRRSGSTANQPARTDGGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQK0</accession>
    <name>Q3SQK0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">2-hydroxychromene-2-carboxylate isomerase</fullName>
            <ecNumber evidence="1">5.99.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2187</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-hydroxychromene-2-carboxylate = (3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate</text>
            <dbReference type="Rhea" id="RHEA:27401"/>
            <dbReference type="ChEBI" id="CHEBI:59350"/>
            <dbReference type="ChEBI" id="CHEBI:59353"/>
            <dbReference type="EC" id="5.99.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily. NadH family.</text>
    </comment>
    <dbReference type="EC" id="5.99.1.4" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315410.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2187"/>
    <dbReference type="EnsemblBacteria" id="ABA05441">
        <property type="protein sequence ID" value="ABA05441"/>
        <property type="gene ID" value="Nwi_2187"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2187"/>
    <dbReference type="eggNOG" id="COG3917">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069253_1_3_5"/>
    <dbReference type="OMA" id="AFHNIQP"/>
    <dbReference type="OrthoDB" id="996965at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018845">
        <property type="term" value="F:2-hydroxychromene-2-carboxylate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901170">
        <property type="term" value="P:naphthalene catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03022">
        <property type="entry name" value="DsbA_HCCA_Iso"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001853">
        <property type="entry name" value="DSBA-like_thioredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014440">
        <property type="entry name" value="HCCAis_GSTk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044087">
        <property type="entry name" value="NahD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01323">
        <property type="entry name" value="DSBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006386">
        <property type="entry name" value="HCCAis_GSTk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DSBA" evidence="3">
        <location>
            <begin position="2"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR006386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR006386-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="201" mass="22435" checksum="72AE210EB5F31AD2" modified="2005-10-11" version="1">MIEFFFDCASPWTYLAFHNIQPLAKEFGAQISWRPVLAGGIFNAVNPSVSAARENPVAAKVHYLKKDLADWARLAGLTIRMPPTVFPVNSVKAMRGCLWLGNDAMVPFARSVFEAYWRDDKDISREPVLAEICRSLDIDPEMLLTGIGDEGIKNELKANTDEAIARGAFGSPTIFLNKTDMYFGNDRLPLIREALQRKKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQV7</accession>
    <name>Q3SQV7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TonB-dependent receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2076</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TonB-dependent receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315313.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2076"/>
    <dbReference type="EnsemblBacteria" id="ABA05334">
        <property type="protein sequence ID" value="ABA05334"/>
        <property type="gene ID" value="Nwi_2076"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2076"/>
    <dbReference type="HOGENOM" id="CLU_008287_9_3_5"/>
    <dbReference type="OMA" id="YDYADAR"/>
    <dbReference type="OrthoDB" id="439303at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039423">
        <property type="entry name" value="TonB-dep_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000531">
        <property type="entry name" value="TonB-dep_rcpt_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036942">
        <property type="entry name" value="TonB_rcpt_b-brl_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010917">
        <property type="entry name" value="TonB_rcpt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32552">
        <property type="entry name" value="PTHR32552"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00593">
        <property type="entry name" value="TonB_dep_Rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01156">
        <property type="entry name" value="TONB_DEPENDENT_REC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TonB_dep_Rec" evidence="2">
        <location>
            <begin position="43"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="533" mass="59438" checksum="361D3A01C5F2D3D7" modified="2005-10-11" version="1">MTYEDREFFYNVAKSNKYLLYGIVEADVTDSTMLTFGSDIMRQDGSNWRYGLPRYSNGADIGLPRSTFLAPDENRWLRNTTKQFVRLDQELGDKWTLGIEASRAHSKVDEKTQVFWGAIDPITLSGLRSGTGHGRQFYRNETQSTLDAVLKGSFHLLGMDHKLTLGANIIRYDSGGETNARNGGTIFVPNIFEFDPADFRLNDPYFRIFQWATKITQKGVYGSLAAKIADPLTLIVGGRLSWYNYDDAITTFDRNTGNITGTTLTRFDDNKVFTPSLGLVYDFSTHWSAYASFAETYQPQANSLSGPLPGTPLGPVTGRTYETGVKGSLLDGRLNTSIALYHVNRNGEAVPDPAYPPVTGDLGRNCCFLGQGRIVSRGVDAEVSGEIAEGWRVQAGYTFNDNENVVAGGRYSTVTPKHLFKLWTTYALRGPLDGWKVGGGVTAQSSFYRKGEARTLNPNTGEYDGPLAPFEFTEPGRAVVDLYVEYKINQHWSATLNVNNVFDKRYYQTMGYADYANWCGPPRNFLLSARARF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQV9</accession>
    <name>Q3SQV9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter, ATPase subunit</fullName>
            <ecNumber evidence="6">3.6.3.25</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_2074</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in beta-(1--&gt;2)glucan export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.25" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2074"/>
    <dbReference type="EnsemblBacteria" id="ABA05332">
        <property type="protein sequence ID" value="ABA05332"/>
        <property type="gene ID" value="Nwi_2074"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2074"/>
    <dbReference type="eggNOG" id="COG1120">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_11_5"/>
    <dbReference type="OMA" id="RCWIAMA"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="11"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="43"/>
            <end position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="276" mass="30218" checksum="56C78B363E8FC5E6" modified="2005-10-11" version="1">MMTSTPHIECLEARTVHFRRGTRVILDDITISLKSGEIVALLGANGAGKSTLFRILLGFLKPERGELRLNDSRLAEFSRRALAQRIAYVPQAHVAPFPYKVRDVVLLGRLAETGIFRAPQAADYRAVDEVLERLAITHLALRRYTEISGGERQLTLIARALAQGAGILILDEPMTGLDYGYQVRLLKHLVDLAGSGHAILFSTHNPEHAVRVATRIVVLRDGAIMADGPPAQIITPSLIRALYGVAVSTLTDVEGQMVIVPTMEHALDARLEPTRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQW1</accession>
    <name>Q3SQW1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">TonB-dependent receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_2072</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the TonB-dependent receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315309.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2072"/>
    <dbReference type="EnsemblBacteria" id="ABA05330">
        <property type="protein sequence ID" value="ABA05330"/>
        <property type="gene ID" value="Nwi_2072"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2072"/>
    <dbReference type="eggNOG" id="COG4772">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021461_0_0_5"/>
    <dbReference type="OMA" id="NYVEYER"/>
    <dbReference type="OrthoDB" id="169533at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012910">
        <property type="entry name" value="Plug_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037066">
        <property type="entry name" value="Plug_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039423">
        <property type="entry name" value="TonB-dep_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000531">
        <property type="entry name" value="TonB-dep_rcpt_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036942">
        <property type="entry name" value="TonB_rcpt_b-brl_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32552">
        <property type="entry name" value="PTHR32552"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07715">
        <property type="entry name" value="Plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00593">
        <property type="entry name" value="TonB_dep_Rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0798">TonB box</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229151" evidence="7">
        <location>
            <begin position="21"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="domain" description="Plug" evidence="9">
        <location>
            <begin position="109"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="TonB_dep_Rec" evidence="8">
        <location>
            <begin position="286"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="31"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA05330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="786" mass="86133" checksum="7C51D27937C96B36" modified="2005-10-11" version="1">MKRILFIGALLGGESYGALAVAQDSTNLPPVTIVSDGQPRNVRQNSTTNARGRTARRARQAVPSNQQPVPQQQQQSGGVRASQNSVLSTTPQLAGASSVTQQGIAILGGPAQTSFYQPLALIPSVSVQTPDPYGLNTTRNINIRGKGDFHLSRTIDGLPLMGIVGGSDLFDLENIGRIDVYRGAVPSDKGIGLSNATGVINQLTLRPQDKAGFTARQAFGTDSFYKTFVRIDSGLNPETATKAFLSGSNIGVDKWTGAGDQKRQNVTFGLSQDFGDRITLDVTAVYNNYAENFFRALTYPQTTNLRNNYSHDFNTTLTGVAATDVNYYKFNRMNAKTFATFAKLDYNFAEGQHLLFKPYYWDNDTTRYNAAGSNVQIWRQQNQNVGSVFEYAGQFPWGTDVVVGYWWQSMKPPPPPTDQRRFTVDAAGGLDFSHWQSLARIDNFSVNSPYFQVSQNFGSTFVTSGLRYMVLGAPQMLYYNTAGISDGTHGQALALNPAIYPDATLAARDYTAWLPNVAIRHDLNPALSVNFSYGRRFGRPDWGPQASNYISNRTVFTARGFSLQSLVDKVRPEISDQFDASLRFSQYGLTVIPTLFYAKYQNKQVKVIDPSIGPNIAYFQGTGSSTGYGFELEANYRFDEQFSVFGSTTLASETFDSDTPTLSGGAMLATKGKQIPNTPQVMIKGGVTYQVDRLAIMPIVRYIGPRFGEAANTQRVSGYTVADLTMSYDLGSHFGVESLNASFSIQNIFDRQYISQISPSDIDLSAGATYFLGAPRTVVGSLSMKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQY1</accession>
    <name>Q3SQY1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2052"/>
    <dbReference type="EnsemblBacteria" id="ABA05310">
        <property type="protein sequence ID" value="ABA05310"/>
        <property type="gene ID" value="Nwi_2052"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2052"/>
    <dbReference type="eggNOG" id="ENOG50331S4">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100616_1_0_5"/>
    <dbReference type="OMA" id="PRMTFSY"/>
    <dbReference type="OrthoDB" id="1666830at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021557">
        <property type="entry name" value="DUF3016"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11454">
        <property type="entry name" value="DUF3016"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229212" evidence="1">
        <location>
            <begin position="21"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="161" mass="18076" checksum="DD9DF47AAE98E59C" modified="2005-10-11" version="1">MFGIATVLAAIVLSSASGNAGVNVRFLGAGRYTDAGSFDTSRASVEAALRSHLDRLGRRYLAPGQTLNIDIVDVDLAGYLQPWRYGASNLRVVTGATPPRIELRYVLTERGRRTRRGEERLTDINFEMNPSARLSSDRFVYEKALLDDWFRRTFSRGRQDF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR29</accession>
    <name>GATC_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit C</fullName>
            <shortName evidence="1">Asp/Glu-ADT subunit C</shortName>
            <ecNumber evidence="1">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gatC</name>
        <name type="ordered locus">Nwi_2003</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + 2 H(+) + L-asparaginyl-tRNA(Asn) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14513"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="Rhea" id="RHEA-COMP:9677"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GatC family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315248.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SR29"/>
    <dbReference type="SMR" id="Q3SR29"/>
    <dbReference type="STRING" id="323098.Nwi_2003"/>
    <dbReference type="EnsemblBacteria" id="ABA05262">
        <property type="protein sequence ID" value="ABA05262"/>
        <property type="gene ID" value="Nwi_2003"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2003"/>
    <dbReference type="eggNOG" id="COG0721">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105899_2_0_5"/>
    <dbReference type="OMA" id="VTPMAMK"/>
    <dbReference type="OrthoDB" id="1780195at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050566">
        <property type="term" value="F:asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00122">
        <property type="entry name" value="GatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003837">
        <property type="entry name" value="Asp/Glu-ADT_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036113">
        <property type="entry name" value="Asp/Glu-ADT_sf_sub_c"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15004">
        <property type="entry name" value="PTHR15004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02686">
        <property type="entry name" value="Glu-tRNAGln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141000">
        <property type="entry name" value="SSF141000"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00135">
        <property type="entry name" value="gatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000016160" description="Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit C">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00122"/>
        </source>
    </evidence>
    <sequence length="95" mass="10445" checksum="711C790FC77679BA" modified="2005-10-11" version="1">MSVDAATVRRIAHLARIAVDDAEVPHLQDELNAILAFVEQLQEVNVDGVEPMTSVTPMAMKMRTDVVNDGEIADRIVANAPVTHDHFFLVPKVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR34</accession>
    <name>Q3SR34_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Patatin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_1998</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315244.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1998"/>
    <dbReference type="EnsemblBacteria" id="ABA05257">
        <property type="protein sequence ID" value="ABA05257"/>
        <property type="gene ID" value="Nwi_1998"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1998"/>
    <dbReference type="eggNOG" id="COG1752">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046679_0_0_5"/>
    <dbReference type="OMA" id="FTDAWNK"/>
    <dbReference type="OrthoDB" id="373926at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="SSF52151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="1 2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229074" evidence="3">
        <location>
            <begin position="24"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="PNPLA" evidence="4">
        <location>
            <begin position="56"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="2">
        <location>
            <begin position="96"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="2">
        <location>
            <begin position="280"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="459" mass="49100" checksum="29AC73CC53E9E58E" modified="2005-10-11" version="1">MRSAFAVIGLCALLLGCASIQNAPVNQPGSSTDLVGQLHENFEERTATDDDLVALSFSGGGTRAAAFSFGVLEELAQTPVRGGGASMIDRIDFISGVSGGSVTAAYFGLRKRAALADFREKFLLRNAEEGLQTDLNLATLGRAFAGGINDSSGLPRWLDANLFHGATFADLRQTVRPRVWINASDIYNRTPFVFGATAFSAMCSDLASYPLANAVAASAAVPVAFAPVVIQTFPGRCKDPLPAWIVKARDNRNAPPMLSAFAKAISRYHDGEIPYIKLLDGGLVDNYGLSGFTIALLSARAPYEPLSPQQAVKIRRGLFVVVDAKTGLSGNWINTIEGPSGVDLVKAAADTAIDASVGAGYTAFERTMGDWQAALIRWRCGLTAAERARYGARPGWNCHDLKFFVGRIGFDQLDPARAARLETVPTRFRLPPQQVDEVIAAGRDALRASSAFRMFAGRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR88</accession>
    <name>Q3SR88_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Resolvase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1943</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315196.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1943"/>
    <dbReference type="EnsemblBacteria" id="ABA05203">
        <property type="protein sequence ID" value="ABA05203"/>
        <property type="gene ID" value="Nwi_1943"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1943"/>
    <dbReference type="eggNOG" id="COG1961">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010686_18_15_5"/>
    <dbReference type="OMA" id="ENVKMGM"/>
    <dbReference type="OrthoDB" id="1791095at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038109">
        <property type="entry name" value="DNA_bind_recomb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011109">
        <property type="entry name" value="DNA_bind_recombinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07508">
        <property type="entry name" value="Recombinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="SSF53041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51737">
        <property type="entry name" value="RECOMBINASE_DNA_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="1">
        <location>
            <begin position="15"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Recombinase" evidence="2">
        <location>
            <begin position="175"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="443" mass="49317" checksum="D16612ACA10DF101" modified="2005-10-11" version="1">MTKPPEKSKVVRKLRCAVYTRKSSEEGLEQEFNSLHAQREACEAYIASQRSEGWVLVRDQYDDGGISGGTLERPALKQLLADIEDGLVDVVVVYKIDRLSRSLMDFAKLVEVFDRNGVTFVSVTQSFNTTTSMGRLTLNILLSFAQFEREVTAERIRDKIRASRQKGMWMGGIVPLGYRVENRKLVIEKSEAATVRMIFERFVTIGSATVLAKSLAAASVRTRRGKLVDKGYLYKLLNNRVYVGEAVHKGESYPGEHQPIIDRALWDKVHAILQESPRKRASNTRAQTPALLKGLIFGPDGAAFSPTHTKKGDRLYRYYVSQSVLKRGADACPIARVPAAEVEMAVIDQLRGLLHAPEIIIGTWRAARSEIEGLPEADVRDALERLDPIWDELFPAEQARIVQLLVERVDVAVNGLSIRLRTNGLTSLASELGAIGSDQKKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRD7</accession>
    <name>Q3SRD7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase FkbM</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1894</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315150.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1894"/>
    <dbReference type="EnsemblBacteria" id="ABA05154">
        <property type="protein sequence ID" value="ABA05154"/>
        <property type="gene ID" value="Nwi_1894"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1894"/>
    <dbReference type="eggNOG" id="COG2242">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080160_0_0_5"/>
    <dbReference type="OMA" id="PYNNNCE"/>
    <dbReference type="OrthoDB" id="1340956at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006342">
        <property type="entry name" value="FkbM_mtfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05050">
        <property type="entry name" value="Methyltransf_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01444">
        <property type="entry name" value="fkbM_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_21" evidence="1">
        <location>
            <begin position="96"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="268" mass="29343" checksum="3073BD0987DC5A20" modified="2005-10-11" version="1">MIAPPFGSFAPSWAQKKLLHAVRRSRLRRGAFRPAMSRLLNLLRPGPIDDTYQGASFRFYHQYSGTERGALLNPDYNLEELRFLRKHTPVGGCFVDIGANVGTYALAMARHVGAEGIVLAIEPHPVAYERLSFNREASGSRNVHLMQAAAGESEGDLLIATDGNNLGASHVVSAGASRDAITVRSVRLRQALESAGIGHVHALKIDVEGYEDRVLVGFFKDAPRSMWPQAIVIEHLSRSEWSTDCIALMLECGYVVAGTTRSNTMLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRT7</accession>
    <name>Q3SRT7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1743</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05004">
        <property type="protein sequence ID" value="ABA05004"/>
        <property type="gene ID" value="Nwi_1743"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1743"/>
    <dbReference type="eggNOG" id="ENOG5030W9E">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155073_0_0_5"/>
    <dbReference type="OMA" id="NGLYSVH"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032417">
        <property type="entry name" value="GrlR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043019">
        <property type="entry name" value="GrlR_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16518">
        <property type="entry name" value="GrlR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="131" mass="14077" checksum="2FE6E270BC6B6486" modified="2005-10-11" version="1">MRPGQDKITGCERITMKNGLYSVHVALLDGRLGKGSGVVMFRDGKILGGDAYLFYTGSYFTKDDGTFRGEVLVQRHTPNPDPNLLFSSPEPVGIGVTGTFTDTTGTMNGTALVGKASQIFRATLRKLADTD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRU9</accession>
    <name>LIPB_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Octanoyltransferase</fullName>
            <ecNumber evidence="1">2.3.1.181</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Lipoate-protein ligase B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Lipoyl/octanoyl transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lipB</name>
        <name type="ordered locus">Nwi_1731</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-lysyl-[protein] + octanoyl-[ACP] = H(+) + holo-[ACP] + N(6)-octanoyl-L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9636"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:9928"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78463"/>
            <dbReference type="ChEBI" id="CHEBI:78809"/>
            <dbReference type="EC" id="2.3.1.181"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">In the reaction, the free carboxyl group of octanoic acid is attached via an amide linkage to the epsilon-amino group of a specific lysine residue of lipoyl domains of lipoate-dependent enzymes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LipB family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.181" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314988.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRU9"/>
    <dbReference type="SMR" id="Q3SRU9"/>
    <dbReference type="STRING" id="323098.Nwi_1731"/>
    <dbReference type="EnsemblBacteria" id="ABA04992">
        <property type="protein sequence ID" value="ABA04992"/>
        <property type="gene ID" value="Nwi_1731"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1731"/>
    <dbReference type="eggNOG" id="COG0321">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035168_3_0_5"/>
    <dbReference type="OMA" id="GEVTYHC"/>
    <dbReference type="OrthoDB" id="1806796at2"/>
    <dbReference type="UniPathway" id="UPA00538">
        <property type="reaction ID" value="UER00592"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033819">
        <property type="term" value="F:lipoyl(octanoyl) transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16444">
        <property type="entry name" value="LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00013">
        <property type="entry name" value="LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000544">
        <property type="entry name" value="Octanoyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020605">
        <property type="entry name" value="Octanoyltransferase_CS"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016262">
        <property type="entry name" value="LPLase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00214">
        <property type="entry name" value="lipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01313">
        <property type="entry name" value="LIPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000242736" description="Octanoyltransferase">
        <location>
            <begin position="1"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="BPL/LPL catalytic" evidence="2">
        <location>
            <begin position="49"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="1">
        <location>
            <position position="193"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="87"/>
            <end position="94"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="162"/>
            <end position="164"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="175"/>
            <end position="177"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Cys" evidence="1">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01067"/>
        </source>
    </evidence>
    <sequence length="231" mass="25737" checksum="DD01C73853B470CD" modified="2005-10-11" version="1">MINSRQTLDLKLFAASPGEPVDWRRSDQPVPYPGAVDAMERRAAAIASSEAAEQVWLLEHPPLYTCGTSGREADLVKARFPLFSTGRGGQITYHGPGQRIAYVMLDLKRRRPDVREFIACLEEWIIRTLDAFGVKGERREDRVGVWVARPDKGPGHEDKIAAIGVRLRRWVSFHGVSLNVDPDLSHFADIVPCGVSDPRYGVTSLADLGLRVRLTDIDQALKRTFSEVFGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRV9</accession>
    <name>Q3SRV9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thiamine-monophosphate kinase</fullName>
            <shortName evidence="2">TMP kinase</shortName>
            <shortName evidence="2">Thiamine-phosphate kinase</shortName>
            <ecNumber evidence="2">2.7.4.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">thiL</name>
        <name evidence="5" type="ordered locus">Nwi_1721</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the ATP-dependent phosphorylation of thiamine-monophosphate (TMP) to form thiamine-pyrophosphate (TPP), the active form of vitamin B1.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + thiamine phosphate = ADP + thiamine diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15913"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine diphosphate from thiamine phosphate: step 1/1.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="2">Reaction mechanism of ThiL seems to utilize a direct, inline transfer of the gamma-phosphate of ATP to TMP rather than a phosphorylated enzyme intermediate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the thiamine-monophosphate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.16" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314978.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1721"/>
    <dbReference type="EnsemblBacteria" id="ABA04982">
        <property type="protein sequence ID" value="ABA04982"/>
        <property type="gene ID" value="Nwi_1721"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1721"/>
    <dbReference type="eggNOG" id="COG0611">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046964_3_0_5"/>
    <dbReference type="OMA" id="HFRRDWS"/>
    <dbReference type="OrthoDB" id="1016556at2"/>
    <dbReference type="UniPathway" id="UPA00060">
        <property type="reaction ID" value="UER00142"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009030">
        <property type="term" value="F:thiamine-phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02194">
        <property type="entry name" value="ThiL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02128">
        <property type="entry name" value="TMP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006283">
        <property type="entry name" value="ThiL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30270">
        <property type="entry name" value="PTHR30270"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005303">
        <property type="entry name" value="Thiam_monoph_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01379">
        <property type="entry name" value="thiL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AIRS" evidence="3">
        <location>
            <begin position="27"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS_C" evidence="4">
        <location>
            <begin position="149"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="28"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="44"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="118"/>
            <end position="119"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="323" mass="33422" checksum="A7F21A5F720F8BA9" modified="2005-10-11" version="1">MPSGEDDLIARYFKPIATDPGALGLTDDAAILKGGGDDLVITTDAIVEGVHFRTSDPPETVARKALRVNLSDLAAKGATPAGFVLTLALRELDERWLAAFASGLSDDAITFDCPLLGGDTVATPGPLTMSITAFGRAPRNGMILRSRAQPGDRIAVSGTIGDAALGLAILSGNVTVSDPRICEPLIERYRIPQPRVHLAPAVRDHARAAMDVSDGLAGDLAKLCRASGVSAIIDAAAVPFSEAAREVIEQGKASFASAVSGGDDYEILCTVAKGHWDDFAQAARSAGVAVTSIGEVVAGNAAPKWRDAKGGDIALPPLSYSHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRW1</accession>
    <name>Q3SRW1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetylmuramoyl-L-alanine amidase</fullName>
            <ecNumber evidence="2">3.5.1.28</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_1719</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.</text>
            <dbReference type="EC" id="3.5.1.28"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.5.1.28" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1719"/>
    <dbReference type="EnsemblBacteria" id="ABA04980">
        <property type="protein sequence ID" value="ABA04980"/>
        <property type="gene ID" value="Nwi_1719"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1719"/>
    <dbReference type="eggNOG" id="COG0860">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014322_2_1_5"/>
    <dbReference type="OMA" id="RYGLVMP"/>
    <dbReference type="OrthoDB" id="1184688at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008745">
        <property type="term" value="F:N-acetylmuramoyl-L-alanine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02696">
        <property type="entry name" value="MurNAc-LAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021731">
        <property type="entry name" value="AMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002508">
        <property type="entry name" value="MurNAc-LAA_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01520">
        <property type="entry name" value="Amidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11741">
        <property type="entry name" value="AMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00646">
        <property type="entry name" value="Ami_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229182" description="N-acetylmuramoyl-L-alanine amidase" evidence="5">
        <location>
            <begin position="27"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="MurNAc-LAA" evidence="6">
        <location>
            <begin position="252"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="160"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="422" mass="45115" checksum="4DCDA2C4C7580ED2" modified="2005-10-11" version="1">MACRANLGIVWLCAAAMLAPGAASLAAGNEPARPEVAVDFPVASAVRVAGDLKQTRFVLDLDRKIDLRAFLLSDPYRVVIDTPQVSFHLAPKAGEAGRGLIKAFRYGLVMPGGSRIVFDLAGPAKIGKAYVLDAANGQPSRLVVELEAVDRAAFNRLLARSSSPEPAKDTGPPGPVADTPGSPGVADGRLVVVIDPGHGGLDNGTQASTGESEKDLVLAFGTALRDRIEQGGRFRVVMTRTDDTFIPLAERVRIARNNGASLFVSIHADALPRHEGDARGATIYTLSDRASDREAERLAEAENKADAIGGVDLTEEPTEVADILIDLARRETRTFSNRFARLLMSEMKTSARMHKHPLKSAGFRVLKAPDVPSVLVELGYVSNNADLRQMASEEWRSKTVRSVAKAIEAFLAKRVVSAAPVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRZ4</accession>
    <name>Q3SRZ4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">3-oxoacyl-[acyl-carrier-protein] synthase 2</fullName>
            <ecNumber evidence="3 10">2.3.1.179</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Nwi_1686</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in the type II fatty acid elongation cycle. Catalyzes the elongation of a wide range of acyl-ACP by the addition of two carbons from malonyl-ACP to an acyl acceptor. Can efficiently catalyze the conversion of palmitoleoyl-ACP (cis-hexadec-9-enoyl-ACP) to cis-vaccenoyl-ACP (cis-octadec-11-enoyl-ACP), an essential step in the thermal regulation of fatty acid composition.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(9Z)-hexadecenoyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxo-(11Z)-octadecenoyl-[ACP] + CO2 + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:55040"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10800"/>
            <dbReference type="Rhea" id="RHEA-COMP:14074"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:83989"/>
            <dbReference type="ChEBI" id="CHEBI:138538"/>
            <dbReference type="EC" id="2.3.1.179"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a fatty acyl-[ACP] + H(+) + malonyl-[ACP] = a 3-oxoacyl-[ACP] + CO2 + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:22836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10 12">Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP synthases family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.179" evidence="3 10"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314946.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1686"/>
    <dbReference type="EnsemblBacteria" id="ABA04947">
        <property type="protein sequence ID" value="ABA04947"/>
        <property type="gene ID" value="Nwi_1686"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1686"/>
    <dbReference type="eggNOG" id="COG0304">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_69_2_5"/>
    <dbReference type="OMA" id="QIGHCLG"/>
    <dbReference type="OrthoDB" id="606297at2"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00834">
        <property type="entry name" value="KAS_I_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017568">
        <property type="entry name" value="3-oxoacyl-ACP_synth-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000794">
        <property type="entry name" value="Beta-ketoacyl_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712">
        <property type="entry name" value="PTHR11712"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000447">
        <property type="entry name" value="KAS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03150">
        <property type="entry name" value="fabF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="B_KETOACYL_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10 14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8 10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 12 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PKS_KS" evidence="13">
        <location>
            <begin position="4"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000447-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABA04947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="421" mass="44348" checksum="61DDA744C57CA328" modified="2005-10-11" version="1">MRRVVVTGLGMVSPLGCGVETTWKRVLDGKSGAKRIETFDVTDMPCRIACIVPRGDGADGTFNPDQWMEPKDQRRVDDFIIFAMSAARQALDDADWHPSTEEDRCATGTLIGSGIGGLSGIADTAVLMKERGARKVSPFFIPGRLINLASGYVSIEHGLKGPNHAVVTACSTGAHAIGDASRLIALGDADVMVAGGAESPICRIGMAGFCAARTLSTSFNDTPHKASRPYDSNRDGFVMGEGAGIVVLEEYEHARRRGARIYAEITGYGLSGDGYHITSPSPDGDGAFRSMTAAMKRAGVSPSDIDYINAHGTSTPVGDEIELKAVERLLGNAASRTAMSSTKSSIGHLLGAAGAVEAIFSILAIRDNVAPPTINLDSPSVETAIDLIPHKARQREINVALSNSFGFGGTNASIIMRRIAN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRZ7</accession>
    <name>KGUA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Guanylate kinase</fullName>
            <ecNumber evidence="1">2.7.4.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">GMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gmk</name>
        <name type="ordered locus">Nwi_1683</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Essential for recycling GMP and indirectly, cGMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + GMP = ADP + GDP</text>
            <dbReference type="Rhea" id="RHEA:20780"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the guanylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.8" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314943.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRZ7"/>
    <dbReference type="SMR" id="Q3SRZ7"/>
    <dbReference type="STRING" id="323098.Nwi_1683"/>
    <dbReference type="EnsemblBacteria" id="ABA04944">
        <property type="protein sequence ID" value="ABA04944"/>
        <property type="gene ID" value="Nwi_1683"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1683"/>
    <dbReference type="eggNOG" id="COG0194">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001715_1_0_5"/>
    <dbReference type="OMA" id="EWAVVHG"/>
    <dbReference type="OrthoDB" id="1502854at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004385">
        <property type="term" value="F:guanylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00328">
        <property type="entry name" value="Guanylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017665">
        <property type="entry name" value="Guanylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03263">
        <property type="entry name" value="guanyl_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000266359" description="Guanylate kinase">
        <location>
            <begin position="1"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="1">
        <location>
            <begin position="15"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="22"/>
            <end position="29"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00328"/>
        </source>
    </evidence>
    <sequence length="219" mass="24913" checksum="68E9239DCF61D6FE" modified="2005-10-11" version="1">MTAHDRGFDGVERRGLMFVLSSPSGAGKTTLSRLLIERVEGLSLSVSATTRPMRPGEVDGRDYAFVDEKTFAAMVERNDLLEWAHVFDNHYGTPRAPVDAALSSGRDVLFDIDWQGTQQLREKARDDVVSVFILPPSAADLERRLHTRAQDSDDVIRERMARAAHEVSHWAEYDYIVINRDIDEAFAEVQSILKAERLKRERRTGLTAFVRELQRQLET</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRZ8</accession>
    <name>RSMA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal RNA small subunit methyltransferase A</fullName>
            <ecNumber evidence="1">2.1.1.182</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">16S rRNA dimethyladenosine transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">16S rRNA dimethylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rsmA</name>
        <name evidence="1" type="synonym">ksgA</name>
        <name type="ordered locus">Nwi_1682</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosine(1518)/adenosine(1519) in 16S rRNA + 4 S-adenosyl-L-methionine = 4 H(+) + N(6)-dimethyladenosine(1518)/N(6)-dimethyladenosine(1519) in 16S rRNA + 4 S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:19609"/>
            <dbReference type="Rhea" id="RHEA-COMP:10232"/>
            <dbReference type="Rhea" id="RHEA-COMP:10233"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74493"/>
            <dbReference type="EC" id="2.1.1.182"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.182" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314942.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRZ8"/>
    <dbReference type="SMR" id="Q3SRZ8"/>
    <dbReference type="STRING" id="323098.Nwi_1682"/>
    <dbReference type="EnsemblBacteria" id="ABA04943">
        <property type="protein sequence ID" value="ABA04943"/>
        <property type="gene ID" value="Nwi_1682"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1682"/>
    <dbReference type="eggNOG" id="COG0030">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041220_0_1_5"/>
    <dbReference type="OMA" id="KEEEPYF"/>
    <dbReference type="OrthoDB" id="2030110at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052908">
        <property type="term" value="F:16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00607">
        <property type="entry name" value="16SrRNA_methyltr_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023165">
        <property type="entry name" value="rRNA_Ade_diMease-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020596">
        <property type="entry name" value="rRNA_Ade_Mease_Trfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020598">
        <property type="entry name" value="rRNA_Ade_methylase_Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011530">
        <property type="entry name" value="rRNA_adenine_dimethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="PTHR11727"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00650">
        <property type="entry name" value="rADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00755">
        <property type="entry name" value="ksgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01131">
        <property type="entry name" value="RRNA_A_DIMETH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51689">
        <property type="entry name" value="SAM_RNA_A_N6_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000257313" description="Ribosomal RNA small subunit methyltransferase A">
        <location>
            <begin position="1"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00607"/>
        </source>
    </evidence>
    <sequence length="287" mass="31397" checksum="F7319885E86E3BE6" modified="2005-10-11" version="1">MSMIDDLPPLRDVIKRHALSARKSLGQNFLLDLNLTSRIARAAGPLQDATVVEIGPGPGGLTRALLAVGAKHVIAIERDERALGALEEISDRYPGRLTIINADATDFDPRPLLGTTRAKIVANLPYNIATALLIRWLSIEPWPPWYDVMVLMFQREVAERIVARENEDAYGRLGVLSNWRAETKILFDISPAAFVPQPQVTSSVVRLIPRYNPEPCDRRSLEQVAAAAFGHRRKMLRQSLKSLPADPASLAAAAGIDPTRRAETVSISGFAAMARELASIVGSGKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS29</accession>
    <name>Q3SS29_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">N-acetyl-gamma-glutamyl-phosphate reductase</fullName>
            <shortName evidence="4">AGPR</shortName>
            <ecNumber evidence="4">1.2.1.38</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">N-acetyl-glutamate semialdehyde dehydrogenase</fullName>
            <shortName evidence="4">NAGSA dehydrogenase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">argC</name>
        <name evidence="7" type="ordered locus">Nwi_1651</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the NADPH-dependent reduction of N-acetyl-5-glutamyl phosphate to yield N-acetyl-L-glutamate 5-semialdehyde.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>N-acetyl-L-glutamate 5-semialdehyde + NADP(+) + phosphate = H(+) + N-acetyl-L-glutamyl 5-phosphate + NADPH</text>
            <dbReference type="Rhea" id="RHEA:21588"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29123"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57936"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.38"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 3/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NAGSA dehydrogenase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.38" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314911.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1651"/>
    <dbReference type="EnsemblBacteria" id="ABA04912">
        <property type="protein sequence ID" value="ABA04912"/>
        <property type="gene ID" value="Nwi_1651"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1651"/>
    <dbReference type="eggNOG" id="COG0002">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077118_0_0_5"/>
    <dbReference type="OMA" id="FSWRNNN"/>
    <dbReference type="OrthoDB" id="951261at2"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00108"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003942">
        <property type="term" value="F:N-acetyl-gamma-glutamyl-phosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01110">
        <property type="entry name" value="ArgC_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023013">
        <property type="entry name" value="AGPR_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010136">
        <property type="entry name" value="AGPR_type-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01851">
        <property type="entry name" value="argC_other"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01224">
        <property type="entry name" value="ARGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0521">NADP</keyword>
    <keyword evidence="3 4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Semialdhyde_dh" evidence="6">
        <location>
            <begin position="21"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="active site" evidence="4 5">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="342" mass="36342" checksum="6E80F80642A76746" modified="2005-10-11" version="1">MTSADTTMKGVSSSGAAGKPTVFVDGASGTTGLEIRQRLDRQGDVVVKSLSDDKRKDPAAKRALMAEVDLVILCLPDEAAKETVALIDGMGADAPKVLDASTAFRVASDWTYGFPELTPDQADRIKAARKVANPGCYPTGSIALLRPLTDAGLIPPDYPVTINAVSGYSGGGKSMIESFEKGAAPAFELYGLGFEHKHLPETQKYANLTRRPIFAPSVGDYRQGMLVSIPLHLDTLPGQPDGATLHATLAKRYAGSTYVSVMPLDNAAAKSGRIEPEALNGSNRLELHVFATDRHRQAVLVARLDNLGKGASGAAVQNMRLMLGLPDRDDAATPTEQEWNEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSH0</accession>
    <name>Q3SSH0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Extracellular solute-binding protein, family 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_1510</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial solute-binding protein 5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1510"/>
    <dbReference type="EnsemblBacteria" id="ABA04771">
        <property type="protein sequence ID" value="ABA04771"/>
        <property type="gene ID" value="Nwi_1510"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1510"/>
    <dbReference type="eggNOG" id="COG4166">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023171_0_0_5"/>
    <dbReference type="OMA" id="RNYMGAK"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030288">
        <property type="term" value="C:outer membrane-bounded periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030678">
        <property type="entry name" value="Peptide/Ni-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PTHR30290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002741">
        <property type="entry name" value="MppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0574">Periplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SBP_bac_5" evidence="4">
        <location>
            <begin position="119"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="620" mass="70024" checksum="F48FC0178B8D9E22" modified="2005-10-11" version="1">MSKFESKWTRYCRPPRERCRTVVGLALIVGLLAAGYQEVSAADSGYAIAMHGEPALPPDFSRMPYADPDAPKGGRLVQSVPGSFDSLNPFIVKGVALQQIRGFVVESLMARGHDEPFTLYGLLAERIETDDARTYATFHLNPQARFYDGRSVRAEDVLFSWQLLRDKGRPNHRQYYSKVATAEAIDERTVRFDFGGTTDRELPLILGLMPVLPKHAVDAATFEQTSMTAPLGSGPYRVTAVKPGASVTLTRNPDYWGRNLPVNRGLWNFDEIRFDYYREANSQFEAFKRGLYDFRVESEPLRWHEGYNFPAAGDGQLVREAIKTGLPAPSEYLVFNTRRPMFSDIRVREALTLLFDFEWINRNYFFGLYSRAGGFFAGSELSAYARAAADGERSLLKPFPSAVRSDILDGSYRLPVTDGSGRDREALRAALALLSQAGYEFDGTVLRHRSTKAPLAFEILVTTRDQERIALAYARDLKRAGIEVSVRSVDGVQFDQRRLAFDFDMIQNRWDQSLSPGNEQSFYWGSEAADIPGTRNYMGVKNPAIDAMIAAMLKARERPGFVDAVRALDRVLMSGFYVIPVYNVREQWIARWNRIERPAATALTGYIPETWWRRPPKPGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSI2</accession>
    <name>Q3SSI2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1498</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314765.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1498"/>
    <dbReference type="EnsemblBacteria" id="ABA04759">
        <property type="protein sequence ID" value="ABA04759"/>
        <property type="gene ID" value="Nwi_1498"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1498"/>
    <dbReference type="eggNOG" id="ENOG5030VHG">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110603_0_0_5"/>
    <dbReference type="OMA" id="ALRFKWR"/>
    <dbReference type="OrthoDB" id="1880285at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="142" mass="15274" checksum="1843DD7AFCE05CFB" modified="2005-10-11" version="1">MSENDPTDHALAAIASFIDKPDVSAEGDADAAPEPEPAEQAVSETATVIPDSPDSKQAETYTRYGPGPLTAVRFKWSTRRGDNGDYFVDETIGESSYPLVSGPMTKEAAIKFVDDRERDARERFEALRSEMAGGETGQSAGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSN9</accession>
    <name>Q3SSN9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CinA protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1441</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314710.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1441"/>
    <dbReference type="EnsemblBacteria" id="ABA04702">
        <property type="protein sequence ID" value="ABA04702"/>
        <property type="gene ID" value="Nwi_1441"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1441"/>
    <dbReference type="eggNOG" id="COG1546">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030805_1_1_5"/>
    <dbReference type="OMA" id="VEHALNM"/>
    <dbReference type="OrthoDB" id="1834406at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036653">
        <property type="entry name" value="CinA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008136">
        <property type="entry name" value="CinA_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02464">
        <property type="entry name" value="CinA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142433">
        <property type="entry name" value="SSF142433"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00199">
        <property type="entry name" value="PncC_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CinA" evidence="2">
        <location>
            <begin position="9"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="208" mass="21975" checksum="456631E9319A09D5" modified="2005-10-11" version="1">MNNSDARALSRSLLDICRMRKLTIATAESCTGGLVAAALTEIPGSSDVIDRGFVTYSNNAKRAMLGVKAGTLDSFGAVSKETATAMAIGALERADTDLAVSITGIAGPGGATPGKPVGLVHFAAAARDGRLIHRECRFGAIGRSSVRQRSVVEALRMLMELTRGPKPPAKVRRESISRSRPRAARAPTRRASERRRTAAPVKPSPKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSS0</accession>
    <name>Q3SSS0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Saccharopine dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314680.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04671">
        <property type="protein sequence ID" value="ABA04671"/>
        <property type="gene ID" value="Nwi_1410"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1410"/>
    <dbReference type="eggNOG" id="COG3268">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031002_0_2_5"/>
    <dbReference type="OMA" id="GPYQLYG"/>
    <dbReference type="OrthoDB" id="1006830at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sacchrp_dh_NADP" evidence="1">
        <location>
            <begin position="10"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="393" mass="41783" checksum="4C32C6711451318B" modified="2005-10-11" version="1">MTSSPAEFDIVVYGASGFTGRLVAEYLAARYGGGDLKWAIAGRDLDKLASVREAIGAPRDLPLVAADAGNPASLRSMAARTASVLSTVGPYQLYGSDLIAVCAASGTDYLDLCGEPIWMRQMIDAHEAEARRSGARIVFSCGYDSLPFELGVFFLQQAAKDRFGSALSRVKGRVRKMKGTFSGGTAASMKAIFSAAANDPSLLPKLRDSFVLTPGFEGPKQPPGNKPLFDEDLGMWVAPFVMANINTRNVHRSNFLLGFPYGQDFVYDEMVVAGPKETGEATAKAIIAANNKLGAQGGPKPGEGPSKEERESGHYDLLFLGIGSDGRQLKAIVTGDRDPGYGSTSKMIAECAICLRHDTPDVAGGFWTPGAAMKDALIKRLTNHAGLTFTIEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSY7</accession>
    <name>Q3SSY7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PRC-barrel protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1343</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314622.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1343"/>
    <dbReference type="EnsemblBacteria" id="ABA04604">
        <property type="protein sequence ID" value="ABA04604"/>
        <property type="gene ID" value="Nwi_1343"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1343"/>
    <dbReference type="eggNOG" id="COG1873">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108884_2_0_5"/>
    <dbReference type="OMA" id="MEGRGIN"/>
    <dbReference type="OrthoDB" id="1849143at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="SSF50346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRC" evidence="1">
        <location>
            <begin position="17"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="119" mass="13028" checksum="01DE983E7A113C40" modified="2005-10-11" version="1">MATEETIDHLISDRESGKAVYGAENKRIGSIESVMIDKTSGEIAYAVLSFGGFLGIGKEHYLVPWAVLRYDTALGEYGSDISGSRMKGGPKHGTETIFDWNARYAKLDGYHNEVVVRPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="126" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST26</accession>
    <name>Q3ST26_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphoribosylaminoimidazole-succinocarboxamide synthase</fullName>
            <ecNumber evidence="8">6.3.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">SAICAR synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">purC</name>
        <name evidence="10" type="ordered locus">Nwi_1304</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + ATP + L-aspartate = (2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate + ADP + 2 H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:22628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the SAICAR synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.6" evidence="8"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_041344884.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1304"/>
    <dbReference type="EnsemblBacteria" id="ABA04565">
        <property type="protein sequence ID" value="ABA04565"/>
        <property type="gene ID" value="Nwi_1304"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1304"/>
    <dbReference type="eggNOG" id="COG0152">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061495_2_0_5"/>
    <dbReference type="OMA" id="EFCYKND"/>
    <dbReference type="OrthoDB" id="1345271at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00131"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004639">
        <property type="term" value="F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01415">
        <property type="entry name" value="SAICAR_synt_PurC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00137">
        <property type="entry name" value="SAICAR_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028923">
        <property type="entry name" value="SAICAR_synt/ADE2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033934">
        <property type="entry name" value="SAICAR_synt_PurC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001636">
        <property type="entry name" value="SAICAR_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018236">
        <property type="entry name" value="SAICAR_synthetase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01259">
        <property type="entry name" value="SAICAR_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00081">
        <property type="entry name" value="purC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01057">
        <property type="entry name" value="SAICAR_SYNTHETASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAICAR_synt" evidence="9">
        <location>
            <begin position="17"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA04565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="265" mass="30349" checksum="38C40D29ECAAB86C" modified="2005-10-11" version="1">MDFNNTRYIPMSRRRRIYEGKAKVLYEGPEPGTLIQHFKDDATAFNAKKHQVIEGKGVLNNRISEYLFQHLNDIGVPTHFIRRLNMREQLIREVEIVPLEVVVRNVAAGSLSQRLGIEEGTQLPRSIIEFYYKNDQLNDPMVSEEHVTAFGWATPQEIDDIMALAIRVNDFLTGLFLGIGIRLVDFKMECGRLFENEMMRIVVADEISPDSCRLWDIKSNEKLDKDVFRRDLGGLLEAYTEVAKRLGILMENERPIGAGPVLVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST68</accession>
    <name>Q3ST68_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidase_M78 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1262</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314551.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1262"/>
    <dbReference type="EnsemblBacteria" id="ABA04523">
        <property type="protein sequence ID" value="ABA04523"/>
        <property type="gene ID" value="Nwi_1262"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1262"/>
    <dbReference type="eggNOG" id="COG2856">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083560_1_0_5"/>
    <dbReference type="OMA" id="MPAEMFR"/>
    <dbReference type="OrthoDB" id="1840229at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010359">
        <property type="entry name" value="IrrE_HExxH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06114">
        <property type="entry name" value="Peptidase_M78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M78" evidence="1">
        <location>
            <begin position="94"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="255" mass="29284" checksum="455D4CB3B28CC1A8" modified="2005-10-11" version="1">MSRFVPYLAEEAIERDAASLIGEYERARGVMIEPPVPVEDIVEKYLKLRIEFDDMHARHNIPRPPNGQPDILGAIYGDGSIFIDESLDPEENPTREGRYRFTLAHEGGGHWRLHQHLIVQNTAQASFLDAASEPKFICRSSEAKERVEWQADFYASCLLMPRKMVFAMWDDLFPDRKQRVLQPEKPIAHAFVEVPRIHASVGDFDISETEDEALERFCKPLAERFVVSPIAMRIRLEKLGLLHRVVPLQQLLSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST79</accession>
    <name>Q3ST79_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1251</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1251"/>
    <dbReference type="EnsemblBacteria" id="ABA04512">
        <property type="protein sequence ID" value="ABA04512"/>
        <property type="gene ID" value="Nwi_1251"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1251"/>
    <dbReference type="eggNOG" id="ENOG502Z8PI">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1057302_0_0_5"/>
    <dbReference type="OMA" id="REGWIFG"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="286" mass="31676" checksum="C5CF7A0EA2625E5A" modified="2005-10-11" version="1">MPAFASHIGIDYSGAETPTASLKGLRVYLAEGDRSPVEVPPPPSPRKYWTRRGIAEWLVARLGEDVPTLVGIDHGFSFPLRYFEAQGLLPDWPTFLDDFQRHWPTDEDHTYVDFVRDGAAGNGAARMGSARWRRLTEERAGGAKSVFHFDVQGSVAKSTHAGIPWLRFIRQRLGARVHFWPFDGWDIPAGRSAIAEVYPALWSRGFAPEGRTGDQHDAFSISAWLSSADRDGRLAAFLNPDLSPPERTLAQVEGWILGVPGLIRSAKGRDGSSRSEACDPSVFRLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST84</accession>
    <name>Q3ST84_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">3-hydroxymyristoyl-acyl carrier protein dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1245</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314536.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1245"/>
    <dbReference type="EnsemblBacteria" id="ABA04507">
        <property type="protein sequence ID" value="ABA04507"/>
        <property type="gene ID" value="Nwi_1245"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1245"/>
    <dbReference type="eggNOG" id="COG0764">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078912_3_3_5"/>
    <dbReference type="OMA" id="GAKMRTF"/>
    <dbReference type="OrthoDB" id="1763197at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013114">
        <property type="entry name" value="FabA_FabZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30272">
        <property type="entry name" value="PTHR30272"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07977">
        <property type="entry name" value="FabA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="157" mass="17577" checksum="A5B0FA0EAEEE9C3A" modified="2005-10-11" version="1">MHLEYFHLIDRIVDLKTEQRSITVEAQVPQESTIFEGHFPGHPLMPGVLLIESMAQASGWLLIALTRFERMPFLAAVKEAKMRTFVKPGELLTIEAAVVHEGSGFTITEAKVSVGGKTKCNATLTFRQVPFPNDDLRGHMETMARRIGFPQQMFAHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST97</accession>
    <name>Q3ST97_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cytokinin riboside 5'-monophosphate phosphoribohydrolase</fullName>
            <ecNumber evidence="2">3.2.2.n1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1232</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + H2O = adenine + D-ribose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:20129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.2.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LOG family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.n1" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314523.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1232"/>
    <dbReference type="EnsemblBacteria" id="ABA04494">
        <property type="protein sequence ID" value="ABA04494"/>
        <property type="gene ID" value="Nwi_1232"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1232"/>
    <dbReference type="eggNOG" id="COG1611">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058336_4_0_5"/>
    <dbReference type="OMA" id="HQKPIGL"/>
    <dbReference type="OrthoDB" id="1255127at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008714">
        <property type="term" value="F:AMP nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009691">
        <property type="term" value="P:cytokinin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005269">
        <property type="entry name" value="LOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031100">
        <property type="entry name" value="LOG_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03641">
        <property type="entry name" value="Lysine_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00730">
        <property type="entry name" value="TIGR00730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0203">Cytokinin biosynthesis</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="201" mass="21693" checksum="DE984540041BC957" modified="2005-10-11" version="1">MNTIRTVCVYCGSGPGTNPQFVEAAVDFGKALARNNVGLVYGGGSVGLMGAIATSTLDHGGTVTGIIPDFLTAREHALSRVQEMIVTRDMHERKRLMFERSDAFVALPGGVGTLEELVEQLTWQQLGRHSKPIMLANIDGFWEPLLALLAHMRSTAFIRPTLAVNVLQANRVEDILPKLRAAATGVPDSSKQMPADVADRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STI5</accession>
    <name>Q3STI5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase IS3/IS911</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0869</name>
        <name evidence="2" type="ordered locus">Nwi_1144</name>
        <name evidence="3" type="ordered locus">Nwi_2880</name>
        <name evidence="4" type="ordered locus">Nwi_2942</name>
        <name evidence="5" type="ordered locus">Nwi_3105</name>
    </gene>
    <organism evidence="2 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="2 6" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="2">Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0869"/>
    <dbReference type="EnsemblBacteria" id="ABA04133">
        <property type="protein sequence ID" value="ABA04133"/>
        <property type="gene ID" value="Nwi_0869"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04406">
        <property type="protein sequence ID" value="ABA04406"/>
        <property type="gene ID" value="Nwi_1144"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06130">
        <property type="protein sequence ID" value="ABA06130"/>
        <property type="gene ID" value="Nwi_2880"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06192">
        <property type="protein sequence ID" value="ABA06192"/>
        <property type="gene ID" value="Nwi_2942"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06352">
        <property type="protein sequence ID" value="ABA06352"/>
        <property type="gene ID" value="Nwi_3105"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0869"/>
    <dbReference type="KEGG" id="nwi:Nwi_1144"/>
    <dbReference type="KEGG" id="nwi:Nwi_2880"/>
    <dbReference type="KEGG" id="nwi:Nwi_2942"/>
    <dbReference type="KEGG" id="nwi:Nwi_3105"/>
    <dbReference type="eggNOG" id="COG2963">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027402_36_0_5"/>
    <dbReference type="OMA" id="YCSWSKE"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002514">
        <property type="entry name" value="Transposase_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010921">
        <property type="entry name" value="Trp_repressor/repl_initiator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01527">
        <property type="entry name" value="HTH_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48295">
        <property type="entry name" value="SSF48295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA06192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA06352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="124" mass="13919" checksum="94A23E4E2A36F84C" modified="2005-10-11" version="1">MRQKSGPGKAPAEQVLKDIRRQTRRHYSAEEKIRIVLEGLRGEENISELCRREGIAASMYYGWSKEFLEAGKRRLAGDTARAATSGEVKDLRREASALKEVVADLTLENRLLKKSMNGDGENEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STL2</accession>
    <name>Q3STL2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Patatin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_1117</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NTE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314410.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1117"/>
    <dbReference type="EnsemblBacteria" id="ABA04379">
        <property type="protein sequence ID" value="ABA04379"/>
        <property type="gene ID" value="Nwi_1117"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1117"/>
    <dbReference type="eggNOG" id="COG0664">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1752">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_1_3_5"/>
    <dbReference type="OMA" id="AGEMAMI"/>
    <dbReference type="OrthoDB" id="103939at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="SSF51206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="SSF52151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3 4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="5">
        <location>
            <begin position="21"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="PNPLA" evidence="6">
        <location>
            <begin position="333"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="4">
        <location>
            <begin position="337"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="4">
        <location>
            <begin position="364"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="366"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="621" mass="67855" checksum="51FE6B8F722AA2DC" modified="2005-10-11" version="1">MKSDPDLQQRDALLQTVLRDYFASDNAEILHTIRSVAEYVDLVSGAILLRQGDISNDVYFILSGRLHAFSETESGARKILGEIGRGETIGELALFTGEPRSATVVAVRDTLLVKVTRAMIERTILQHPEIEMSMMRAVIRRFRQRERERQAPLVPVKICILPITSGPDAAGFARSLRNAQAAEAGPVAVVTKDDVASHFGAQDQAPGKRNDALAGYIDDIEARNRAVYLAADGGDTAWTRFCLRHADEVLLLANAGRDPGLSAVERACLSPAAPISIARQTLVLLHRADTRIPTSTAHWLNARPDARHFHIRPELPADMRRLARIVSGRAIGLVLSGGGARGFAHVGVIKALEEAGIPVDMLGGSSIGAVMGMVLALGRSAEEVTAAVRKAFLEHPRGNVTGDYNFIPLVSLIKGARTRSAMTQAVRDATGRDAIDSEDCWTTFFTIASDFSTGSEAVLQRGDLTRNVGASYAIPGALPPVFVGGHMMYDGSTFNNFPVDVMARLGAGRIIGVDLSVDREQLFDIDDVPGTLTLLRDKLRPRAKRRYRLPSVPETMLRSSFITSISKQKEMGKFTDLLFRPRISDGRLLDWSRFEEVVAAGYSQAREMLAGMSDEQRKMFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STP8</accession>
    <name>Q3STP8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, AsnC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1081</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314377.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1081"/>
    <dbReference type="EnsemblBacteria" id="ABA04343">
        <property type="protein sequence ID" value="ABA04343"/>
        <property type="gene ID" value="Nwi_1081"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1081"/>
    <dbReference type="eggNOG" id="COG1522">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170329_0_0_5"/>
    <dbReference type="OMA" id="THTIITF"/>
    <dbReference type="OrthoDB" id="1855714at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AsnC_trans_reg" evidence="1">
        <location>
            <begin position="7"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="81" mass="8906" checksum="B8C12715470B2BBC" modified="2005-10-11" version="1">MVPFFVQFKCKLGQSYAVANALAEAEIASEIYSTAGDYDLLAKFYVDNATDIGHFINEKVQVIPGIQDTHTIITFKAFGAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STQ5</accession>
    <name>Q3STQ5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NAD-dependent epimerase/dehydratase</fullName>
            <ecNumber evidence="3">5.1.3.12</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1074</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.12" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314370.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1074"/>
    <dbReference type="EnsemblBacteria" id="ABA04336">
        <property type="protein sequence ID" value="ABA04336"/>
        <property type="gene ID" value="Nwi_1074"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1074"/>
    <dbReference type="eggNOG" id="COG0451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_7_5"/>
    <dbReference type="OMA" id="EPIKVFN"/>
    <dbReference type="OrthoDB" id="1180629at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="2">
        <location>
            <begin position="6"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="339" mass="37594" checksum="BFC8B6D48AF5BFA4" modified="2005-10-11" version="1">MPDHPILVTGAAGFIGFHVAARLLKQGHRVVGIDSLNDYYDPALKECRLEILRGDSRFRFVKSDLADREATAALFAEHHLSVVLHLAAQAGVRYSLRNPHAYVDSNLTAFANVLEGCRHASCPHLLFASSSSVYGANTKLPFSVHDNVDHPISLYAATKKSNELMAHAYSHLYRVPTTGLRFFTVYGPWYRPDMALYKFADAIVGGQPIKLFNHGNMQRDFTFVDDVVEAVVRLIDRAPQPHASWSGDASDAGTSSAPWRIYNIGNNKPAELMGVVALLEKALGRSAQKELLPMQPGDVQATFADIDDLARDVGFRPSTSLEDGIHRFADWYCRYHRVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STT9</accession>
    <name>Q3STT9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CsbD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1040</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0337 (CsbD) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314336.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04302">
        <property type="protein sequence ID" value="ABA04302"/>
        <property type="gene ID" value="Nwi_1040"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1040"/>
    <dbReference type="eggNOG" id="COG3237">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135567_4_1_5"/>
    <dbReference type="OMA" id="FTFDDLW"/>
    <dbReference type="OrthoDB" id="2089955at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008462">
        <property type="entry name" value="CsbD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036629">
        <property type="entry name" value="YjbJ_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05532">
        <property type="entry name" value="CsbD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69047">
        <property type="entry name" value="SSF69047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CsbD" evidence="2">
        <location>
            <begin position="4"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="80" mass="10061" checksum="B39DB3FB45C5998B" modified="2005-10-11" version="1">MDWDRIEQNWDQVKYRVRDQWGKFTFDDLWQISGNREQLERKLKERYGFHEDFAKKQVDDWYEKAQSWPDTKGPARWQGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STY1</accession>
    <name>Q3STY1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0998</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314299.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0998"/>
    <dbReference type="EnsemblBacteria" id="ABA04260">
        <property type="protein sequence ID" value="ABA04260"/>
        <property type="gene ID" value="Nwi_0998"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0998"/>
    <dbReference type="eggNOG" id="ENOG5033BP4">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1693638_0_0_5"/>
    <dbReference type="OMA" id="AYEVRYW"/>
    <dbReference type="OrthoDB" id="1777349at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022037">
        <property type="entry name" value="DUF3606"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12244">
        <property type="entry name" value="DUF3606"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="155" mass="17133" checksum="39C2B0EDD96FD512" modified="2005-10-11" version="1">MADDKAKRGGSDGRLIALREAYEVRYWSKKFNVTPSKLKAAVKAVGRSAKTVEGYLKLQKRATNDRALIALTQPYEVRYWAKKFKVTPARLRTAVGEVGRSAKKVGAFLAARKTVKKKTAVKKKTAAKKVKTKKVKTKTPKATKAKKSAGKRARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU35</accession>
    <name>Q3SU35_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0944</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314247.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04206">
        <property type="protein sequence ID" value="ABA04206"/>
        <property type="gene ID" value="Nwi_0944"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0944"/>
    <dbReference type="eggNOG" id="ENOG50327S1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1783176_0_0_5"/>
    <dbReference type="OMA" id="YPLYQWT"/>
    <dbReference type="OrthoDB" id="1807658at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="146" mass="16674" checksum="E8D486019AEE14D2" modified="2005-10-11" version="1">MVPQWQYQVRFDVVDAAISESIRRKAQDPALTRLFEILARHRAVPKSQFDAFSEYVTTAEERGVDGYPLYQWTKATIEDPAKKEKYLKSFTLYVDDQEVYAKEIADSLESDLQPLAAGGLITRISKYDTNPENNPQPPRRGSKNSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU43</accession>
    <name>Q3SU43_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0936</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314239.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0936"/>
    <dbReference type="EnsemblBacteria" id="ABA04198">
        <property type="protein sequence ID" value="ABA04198"/>
        <property type="gene ID" value="Nwi_0936"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0936"/>
    <dbReference type="eggNOG" id="ENOG5030YYP">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145244_0_1_5"/>
    <dbReference type="OrthoDB" id="2024195at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229270" evidence="1">
        <location>
            <begin position="32"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="91" mass="9608" checksum="87085BE10E4A85FE" modified="2005-10-11" version="1">MTAIHAETARQVSAILAVAAILTTLASPSFAFTAEAQRQCTGDAFRLCSSEIPNIGRITACMVKNRSSLSSGCRVELDKGLSARTHRVAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU85</accession>
    <name>Q3SU85_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, ArsR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0892</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314197.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0892"/>
    <dbReference type="EnsemblBacteria" id="ABA04156">
        <property type="protein sequence ID" value="ABA04156"/>
        <property type="gene ID" value="Nwi_0892"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0892"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097806_6_0_5"/>
    <dbReference type="OMA" id="LHAIFCA"/>
    <dbReference type="OrthoDB" id="1772343at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="6"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="131" mass="14753" checksum="40B805E7DFEB8E06" modified="2005-10-11" version="1">MRALWQSREQADFAADRLRKFAQPQRLMILSLLREGEKSVTEIDAATGIGQPALSQQLAELRKAELVTTRRQAKQIYYSLAHESVALCVRSIEAVFNADNPEQALLNAVRHDLRPESEDVVHGAANFARLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUA4</accession>
    <name>Q3SUA4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0873</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314178.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0873"/>
    <dbReference type="EnsemblBacteria" id="ABA04137">
        <property type="protein sequence ID" value="ABA04137"/>
        <property type="gene ID" value="Nwi_0873"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0873"/>
    <dbReference type="eggNOG" id="COG4319">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2684166_0_0_5"/>
    <dbReference type="OrthoDB" id="1644255at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="74" mass="8523" checksum="9F77B240F76B8561" modified="2005-10-11" version="1">MRCAIISKALDDPQRSFHYSLDIKDVIVDKSIAVVRLDWTLRISPGEAISTETGLDIFRKESDGQWRIIRYIAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUE4</accession>
    <name>Q3SUE4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0832</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0832"/>
    <dbReference type="EnsemblBacteria" id="ABA04097">
        <property type="protein sequence ID" value="ABA04097"/>
        <property type="gene ID" value="Nwi_0832"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0832"/>
    <dbReference type="eggNOG" id="ENOG5030XAI">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133812_0_0_5"/>
    <dbReference type="OrthoDB" id="1818854at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="131" mass="14767" checksum="62945AC81575BF2B" modified="2005-10-11" version="1">MNMKRTLNELVRAVVDEAERNPEFARRIEEALGLQEKPKKPVLSRASHRRAPAALDPVELARRGELELRARLAELNLEQLKDVVADYGMDPGKLVLKWKTADRIIDRIVEVSIGRARKGEGFLSSGSPRSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUE7</accession>
    <name>Q3SUE7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SMF protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0829</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DprA/Smf family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314142.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0829"/>
    <dbReference type="EnsemblBacteria" id="ABA04094">
        <property type="protein sequence ID" value="ABA04094"/>
        <property type="gene ID" value="Nwi_0829"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0829"/>
    <dbReference type="eggNOG" id="COG0758">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029601_3_0_5"/>
    <dbReference type="OMA" id="FLGGGCD"/>
    <dbReference type="OrthoDB" id="1881437at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009294">
        <property type="term" value="P:DNA-mediated transformation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003488">
        <property type="entry name" value="DprA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43022">
        <property type="entry name" value="PTHR43022"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02481">
        <property type="entry name" value="DNA_processg_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="302" mass="33507" checksum="2981CFC5C459E5D3" modified="2005-10-11" version="1">MQLELISQDFAQRAVSPFLELGAYESLWDEQSASFKSIADLFSKQEGAVPSDFVERSRASSYANDVHARLKDAGIKSYGVRVHGAGDYPERLRVADHPIELLYFQGWWELVNSPRSVAVVGTRKPSAEGLARTRRLVKSLLQDDFTIVSGLAAGVDTAAHTAAIELGGRTIAVLGTPLSKSYPASNAKLQQQIAEHHLLISQVPVKRYSNQKNPTSNNFFFPERNITMSALTDATIIVEAGETSGTLVQARHALKQGRKVFILESNFRNPELKWPHTFEERGAIRVADYDDIRRHIVPVTSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUH9</accession>
    <name>Q3SUH9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phage-related minor tail protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0797</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314110.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0797"/>
    <dbReference type="EnsemblBacteria" id="ABA04062">
        <property type="protein sequence ID" value="ABA04062"/>
        <property type="gene ID" value="Nwi_0797"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0797"/>
    <dbReference type="eggNOG" id="COG3096">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3941">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_281165_0_0_5"/>
    <dbReference type="OMA" id="GIRYREM"/>
    <dbReference type="OrthoDB" id="1034587at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009628">
        <property type="entry name" value="Phage_tape_measure_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06791">
        <property type="entry name" value="TMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="227"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="TMP_2" evidence="3">
        <location>
            <begin position="165"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="459"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="610"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1115" mass="119892" checksum="A84994991C94FD6F" modified="2005-10-11" version="1">MSATNVSIRLGIEGKAEIRRAFEEVGQSGQAAFGAVEKALNRTGAATDREVARLKRLAEAARLAADAEASQKRFNAVLNVDRPIPKSARDSASVFEEAAREAESFQARANALRATLDPLGAAQARLNSELAEYAALARRGVITSAEHAAAQGLARQRFDQTARAITGVGGSSKLTRNQLLTLQYTFNDVVASMSTGMSPMTILMQQGGQVTQAFGGLRGTLTAFGSALGVVGAVAIGVAAAVVGLTAAWVANDASTRSVTTALMGIGRASGTTASELERVAQSSAEAGKISVRSAREMEVAFLRTGKIGAEEMARAISIARNFAVTMGVETKAGAEQLASALTDPVRGADELNSRLAFLDDRTRQYIRTLVDQNNRTEAQRVLLAALVPTLADAEQATNAFGRAWNFVARQASNAFDAIGKAVDRAVDGRTPAEELDLLKWQRERLRENVRGDFVPLMLPVVERRIAEIEAQLADAQRRAEQLAADARANELSVRAGETARDIIPGARELERLQREQAALRAALNDPLTRSKLADVAAVEAAYARVTAELARYRPAVDAATQAIVTQNSATEISIRATLALANAYLESAEAAERAEAGKQGLIDQAREGIDAEARARQVLRERIAEAAAQAGRQVADLTAEAAAQKRVNDAVAGGALASAKAAQAMQVEQALRPLLIAQALAEGETKETLTRIIDRMREAYGRLFAEQERAQALSANEDRRREIELLTRQVSLINATVAARGDALAVMRAEQELQRRGVDLASEEARAYIESARQIEGLNRTLRGQEQLRDQRDEITLLERQVALIGASVSRRSEELAILRAIQQLRQRGIDAASPEGQAAIGNARRVDELNRQLAVRQALEDQKDEITLLQRQIGLIGQSSSERAVIIAQLRTEQGLRTRGIDLASEEGRMIVENAGKIERLTQELQRQDAAYRAIENAVGSALDRFADVLAQGKLDWKSWGDAGRAALQELSREMIKLALLNPLKNLLFGSNLPTFSQGSGLLSGIFSRLFHEGGLVGAGGVGRMVPVAAFAYAPRFHEGAYLRADEVPAILQRGERVLNRKEARAYERGDDRAGGAIVNVTIQTPNPTAFDASRTQIAAGLARAVRSGMRGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUL2</accession>
    <name>Q3SUL2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CoxF protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0226</name>
        <name evidence="3" type="ordered locus">Nwi_0764</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="3">Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313561.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0226"/>
    <dbReference type="EnsemblBacteria" id="ABA03494">
        <property type="protein sequence ID" value="ABA03494"/>
        <property type="gene ID" value="Nwi_0226"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04029">
        <property type="protein sequence ID" value="ABA04029"/>
        <property type="gene ID" value="Nwi_0764"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0226"/>
    <dbReference type="KEGG" id="nwi:Nwi_0764"/>
    <dbReference type="eggNOG" id="ENOG5033A6Y">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_209145_0_0_5"/>
    <dbReference type="OMA" id="TMVKGPI"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="56" mass="6137" checksum="675B1E72907E529A" modified="2005-10-11" version="1">MDSNDKPAGDRIVLTEAQKKRRRERSIAIACALGVLVLLFFAVTFIKGPGVLVRPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUS2</accession>
    <name>Q3SUS2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Biopolymer transport protein ExbB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">Nwi_0703</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Involved in the TonB-dependent energy-dependent transport of various receptor-bound substrates. Protects ExbD from proteolytic degradation and functionally stabilizes TonB.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The accessory proteins ExbB and ExbD seem to form a complex with TonB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the exbB/tolQ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0703"/>
    <dbReference type="EnsemblBacteria" id="ABA03969">
        <property type="protein sequence ID" value="ABA03969"/>
        <property type="gene ID" value="Nwi_0703"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0703"/>
    <dbReference type="eggNOG" id="COG0811">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053325_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002898">
        <property type="entry name" value="MotA_ExbB_proton_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014164">
        <property type="entry name" value="TonB_ExbB_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01618">
        <property type="entry name" value="MotA_ExbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02797">
        <property type="entry name" value="exbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="285"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="331"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="MotA_ExbB" evidence="16">
        <location>
            <begin position="253"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="47"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ABA03969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="400" mass="39847" checksum="B3E3A28A9DF4F096" modified="2005-10-11" version="1">MIALASQQISGTKSMIVRLAKATIHLTFAALLAVLVPSATVFAQHRASSPSTTSSGGGAAATPSASSVNGSAGAASAASASPGPASSAPSATDGSAGAASSQPSASTSPEGASDASSGEGSASSDASAGASSDAAAPSDQSAAPDSAAPSPAPEGSSSTLPQDLSPWGMFMQADIVVKAVMIGLAFASLVTWTVWLAKGIELAASRRRAQSCVRKLERADSLDAARSEIAEGWTCEGTVADLMGAAERELQRSGDLSSEGIKERLAIALSRIEAKAGRDIARGTGLLATIGATAPFVGLFGTVWGIMNAFIGISQTKTTNLAVVAPGIAEALLATALGLVAAIPAVIIYNVFARALSSYRALLSDASGEIIQHISRDLERQERGLGPSVVALSRGRAAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUW6</accession>
    <name>Q3SUW6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase IS3/IS911</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0639</name>
        <name evidence="3" type="ordered locus">Nwi_0659</name>
        <name evidence="4" type="ordered locus">Nwi_0783</name>
        <name evidence="5" type="ordered locus">Nwi_0866</name>
        <name evidence="6" type="ordered locus">Nwi_0871</name>
        <name evidence="7" type="ordered locus">Nwi_1017</name>
        <name evidence="8" type="ordered locus">Nwi_1491</name>
        <name evidence="9" type="ordered locus">Nwi_1931</name>
        <name evidence="10" type="ordered locus">Nwi_1944</name>
        <name evidence="11" type="ordered locus">Nwi_1965</name>
        <name evidence="12" type="ordered locus">Nwi_2046</name>
        <name evidence="13" type="ordered locus">Nwi_2081</name>
        <name evidence="14" type="ordered locus">Nwi_2100</name>
        <name evidence="15" type="ordered locus">Nwi_2136</name>
        <name evidence="16" type="ordered locus">Nwi_2666</name>
        <name evidence="17" type="ordered locus">Nwi_2673</name>
        <name evidence="18" type="ordered locus">Nwi_2820</name>
        <name evidence="19" type="ordered locus">Nwi_2867</name>
    </gene>
    <organism evidence="3 20">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="3 20" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="3">Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0639"/>
    <dbReference type="EnsemblBacteria" id="ABA03906">
        <property type="protein sequence ID" value="ABA03906"/>
        <property type="gene ID" value="Nwi_0639"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03925">
        <property type="protein sequence ID" value="ABA03925"/>
        <property type="gene ID" value="Nwi_0659"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04048">
        <property type="protein sequence ID" value="ABA04048"/>
        <property type="gene ID" value="Nwi_0783"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04130">
        <property type="protein sequence ID" value="ABA04130"/>
        <property type="gene ID" value="Nwi_0866"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04135">
        <property type="protein sequence ID" value="ABA04135"/>
        <property type="gene ID" value="Nwi_0871"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04279">
        <property type="protein sequence ID" value="ABA04279"/>
        <property type="gene ID" value="Nwi_1017"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04752">
        <property type="protein sequence ID" value="ABA04752"/>
        <property type="gene ID" value="Nwi_1491"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05191">
        <property type="protein sequence ID" value="ABA05191"/>
        <property type="gene ID" value="Nwi_1931"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05204">
        <property type="protein sequence ID" value="ABA05204"/>
        <property type="gene ID" value="Nwi_1944"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05225">
        <property type="protein sequence ID" value="ABA05225"/>
        <property type="gene ID" value="Nwi_1965"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05304">
        <property type="protein sequence ID" value="ABA05304"/>
        <property type="gene ID" value="Nwi_2046"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05338">
        <property type="protein sequence ID" value="ABA05338"/>
        <property type="gene ID" value="Nwi_2081"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05357">
        <property type="protein sequence ID" value="ABA05357"/>
        <property type="gene ID" value="Nwi_2100"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05391">
        <property type="protein sequence ID" value="ABA05391"/>
        <property type="gene ID" value="Nwi_2136"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05919">
        <property type="protein sequence ID" value="ABA05919"/>
        <property type="gene ID" value="Nwi_2666"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05926">
        <property type="protein sequence ID" value="ABA05926"/>
        <property type="gene ID" value="Nwi_2673"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06073">
        <property type="protein sequence ID" value="ABA06073"/>
        <property type="gene ID" value="Nwi_2820"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06117">
        <property type="protein sequence ID" value="ABA06117"/>
        <property type="gene ID" value="Nwi_2867"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0639"/>
    <dbReference type="KEGG" id="nwi:Nwi_0659"/>
    <dbReference type="KEGG" id="nwi:Nwi_0783"/>
    <dbReference type="KEGG" id="nwi:Nwi_0866"/>
    <dbReference type="KEGG" id="nwi:Nwi_0871"/>
    <dbReference type="KEGG" id="nwi:Nwi_1017"/>
    <dbReference type="KEGG" id="nwi:Nwi_1491"/>
    <dbReference type="KEGG" id="nwi:Nwi_1931"/>
    <dbReference type="KEGG" id="nwi:Nwi_1944"/>
    <dbReference type="KEGG" id="nwi:Nwi_1965"/>
    <dbReference type="KEGG" id="nwi:Nwi_2046"/>
    <dbReference type="KEGG" id="nwi:Nwi_2081"/>
    <dbReference type="KEGG" id="nwi:Nwi_2100"/>
    <dbReference type="KEGG" id="nwi:Nwi_2136"/>
    <dbReference type="KEGG" id="nwi:Nwi_2666"/>
    <dbReference type="KEGG" id="nwi:Nwi_2673"/>
    <dbReference type="KEGG" id="nwi:Nwi_2820"/>
    <dbReference type="KEGG" id="nwi:Nwi_2867"/>
    <dbReference type="eggNOG" id="COG2963">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027402_36_0_5"/>
    <dbReference type="OMA" id="VWSKEFM"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002514">
        <property type="entry name" value="Transposase_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010921">
        <property type="entry name" value="Trp_repressor/repl_initiator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01527">
        <property type="entry name" value="HTH_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48295">
        <property type="entry name" value="SSF48295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="81"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA04135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA04752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA05204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA05225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA05304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA05338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABA05357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABA05391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ABA05919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ABA05926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ABA06073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ABA06117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="124" mass="13955" checksum="B0E2B7BD53ACBAB4" modified="2005-10-11" version="1">MRQKSGPEKAPAEQVVKDIRRATRRQFSAEEKIRIVLEGVRGEESIAELCRREGIASSMYYGWSKEFLDAGKRRLAGDTARAATSDEVKELRREAQALKEAVADLTLENRLLKKSMLADGEDDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUW8</accession>
    <name>Q3SUW8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">FecR protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0657</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313981.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0657"/>
    <dbReference type="EnsemblBacteria" id="ABA03923">
        <property type="protein sequence ID" value="ABA03923"/>
        <property type="gene ID" value="Nwi_0657"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0657"/>
    <dbReference type="eggNOG" id="COG3712">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050192_0_1_5"/>
    <dbReference type="OMA" id="QQWRQAD"/>
    <dbReference type="OrthoDB" id="1381035at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006860">
        <property type="entry name" value="FecR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032623">
        <property type="entry name" value="FecR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012373">
        <property type="entry name" value="Ferrdict_sens_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30273">
        <property type="entry name" value="PTHR30273"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16220">
        <property type="entry name" value="DUF4880"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04773">
        <property type="entry name" value="FecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018266">
        <property type="entry name" value="FecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4880" evidence="3">
        <location>
            <begin position="23"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="FecR" evidence="2">
        <location>
            <begin position="131"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="337" mass="37177" checksum="3F8785AFE0D94FAA" modified="2005-10-11" version="1">MAHDGAEDDKSGGAFTHPDPIVDEALEWLARRQNAPLELAEQRQFQEWLLRSPRHGEEFRYLEAMWRSPELGKAAEGLPAAARGSVNRRRLLSAARYVQSWSLRGVAAAAILMIAIGAWQYPALMLRWQADYITATGGRAQVSLPDGSTMLLNTASAVSIDFTNGRRQVTLLEGEAFFDVRHDPAHPFRVAAHYGETEVKGTAFAVRTESDQDTIVLERGRVEVSRLSDSGDHVELDPGEMVTATAKALSVVARRDPSEILAWRDGRIIFENQRLAHVLDELRRYYDGIVIVADARVGDLVVTGNYRLDDVPGAIRTLADAAGATMNRLPGGIILLR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUZ5</accession>
    <name>AROC_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chorismate synthase</fullName>
            <shortName evidence="1">CS</shortName>
            <ecNumber evidence="1">4.2.3.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5-enolpyruvylshikimate-3-phosphate phospholyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aroC</name>
        <name type="ordered locus">Nwi_0629</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21020"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="EC" id="4.2.3.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMNH2</name>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
        </cofactor>
        <text evidence="1">Reduced FMN (FMNH(2)).</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 7/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the chorismate synthase family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="ABA03896" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.5" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03896.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313956.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SUZ5"/>
    <dbReference type="SMR" id="Q3SUZ5"/>
    <dbReference type="STRING" id="323098.Nwi_0629"/>
    <dbReference type="EnsemblBacteria" id="ABA03896">
        <property type="protein sequence ID" value="ABA03896"/>
        <property type="gene ID" value="Nwi_0629"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0629"/>
    <dbReference type="eggNOG" id="COG0082">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034547_0_0_5"/>
    <dbReference type="OrthoDB" id="981870at2"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00090"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004107">
        <property type="term" value="F:chorismate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07304">
        <property type="entry name" value="Chorismate_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00300">
        <property type="entry name" value="Chorismate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000453">
        <property type="entry name" value="Chorismate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035904">
        <property type="entry name" value="Chorismate_synth_AroC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020541">
        <property type="entry name" value="Chorismate_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21085">
        <property type="entry name" value="PTHR21085"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01264">
        <property type="entry name" value="Chorismate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001456">
        <property type="entry name" value="Chorismate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103263">
        <property type="entry name" value="SSF103263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00033">
        <property type="entry name" value="aroC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00787">
        <property type="entry name" value="CHORISMATE_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00788">
        <property type="entry name" value="CHORISMATE_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00789">
        <property type="entry name" value="CHORISMATE_SYNTHASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000256307" description="Chorismate synthase">
        <location>
            <begin position="1"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="131"/>
            <end position="133"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="243"/>
            <end position="244"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="307"/>
            <end position="311"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="368" mass="39165" checksum="D82800B60B5B5A5D" modified="2006-10-31" version="2">MSHNTFGHLFRVTTFGESHGVAIGCVVDGCPPMLPLTAEEIQRDLDRRRPGQSRFTTQRQEPDAVKILSGVMPHPGTGEQVTTGAPIGLLIENTDQRSKDYSDIKDKFRPGHADFTYEAKYGIRDYRGGGRSSARETAMRVAAGAIARKVVSGMRVRGALVQMGPHKIDRDKWDWDEIARNPFFCPDKDKAAFFESYLDGIRKSGSSIGAVIEVVADGVPAGLGAPIYAKLDGDLAAALMSINAVKGVEIGAGFGAAALTGEENADEMRSSSSDMGNHGPVFTSNHAGGILGGISTGQPIVARFAVKPTSSILSPRKTVDRNGAETDIFTRGRHDPCVGIRAVPVGEAMVACVLADHVLRHRGQVGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV19</accession>
    <name>Q3SV19_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0605</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0605"/>
    <dbReference type="EnsemblBacteria" id="ABA03872">
        <property type="protein sequence ID" value="ABA03872"/>
        <property type="gene ID" value="Nwi_0605"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0605"/>
    <dbReference type="eggNOG" id="ENOG5033HDV">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2735975_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="71" mass="7295" checksum="B7DA1B296273B744" modified="2005-10-11" version="1">MRSSRCTRAERNGTAGERKQPLGDKLAKSDGVLCPPPGIDPEIRAPTPEGGNTPVIPPPGTPGGDPAARPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV68</accession>
    <name>Q3SV68_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Response regulator receiver</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0556</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0556"/>
    <dbReference type="EnsemblBacteria" id="ABA03823">
        <property type="protein sequence ID" value="ABA03823"/>
        <property type="gene ID" value="Nwi_0556"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0556"/>
    <dbReference type="eggNOG" id="COG0784">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_11_5"/>
    <dbReference type="OMA" id="AMTMVDE"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="24"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="140" mass="15100" checksum="4A6A2C997B492C5E" modified="2005-10-11" version="1">MLMEVMHPLVPDGPASAPAGVPRDLLIVEDDVIIAIDLEQTVAELGVALARTASNVAQALAMIAERAPDFALLDVALSEENSFAIARLLATLKIPFAFVTGYSGDTSFPTEYSDRPRLSKPFSRDELFATLLNWRSGVAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV94</accession>
    <name>Q3SV94_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Methyl-accepting chemotaxis sensory transducer with Pas/Pac sensor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_0530</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0530"/>
    <dbReference type="EnsemblBacteria" id="ABA03797">
        <property type="protein sequence ID" value="ABA03797"/>
        <property type="gene ID" value="Nwi_0530"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0530"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3829">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_26_5"/>
    <dbReference type="OMA" id="AVFEHMW"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0807">Transducer</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="6">
        <location>
            <begin position="30"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="8">
        <location>
            <begin position="223"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="5">
        <location>
            <begin position="313"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="7">
        <location>
            <begin position="465"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA03797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="569" mass="61246" checksum="A0AD14F40AE5B842" modified="2005-10-11" version="1">MASFPVRKNFPVTDEEYPVSDDTLIVSRTDLKGRLTYVNEDFIDAAEFTSEELIGKAHNIVRHPDMPPEAFENLWDTLKAEKPWVGAIKNRRKNGGFYWVLATASPIRTDGQVTGYTSIRTRLAADQRALAEDVYAAIREKRPHPYRIDAGIIRKRSVFDRFGLFTRTMRARMTTLITVQAAFLLACGLLGAAVIGGIWGLALILLAAAGIAVGGMVGRQTLRAFEGPMRHLNETMLNLVHDKFDNRIEIRRDDEIGEALRNLQTVQTLIRFSREEVNSTQRRADAQRKIDMTKIANSFEAAIGEIVQAVATSATELEASASTLASNADHAKDFAARVANGSTEASSNVNSVASATEEMTASVREISQRVQESARMAGEAVDQVHSATERVSALSSAAARIGDIVEMINNIAGQTNLLALNATIEAARAGEAGRGFSVVASEVKALAEQTGKATGEIGQQISGIQSATHESVAAINEIRNSIEKLSGISSTIASAIEEQGATTQEVARNVQHAAQGAQEVSSNVGNLQRGAVETGNASSLVLSAAQTLSRDSARLKTEVDNFLNSVRVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVB2</accession>
    <name>Q3SVB2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Global cell cycle regulator GcrA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0512</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313840.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0512"/>
    <dbReference type="EnsemblBacteria" id="ABA03779">
        <property type="protein sequence ID" value="ABA03779"/>
        <property type="gene ID" value="Nwi_0512"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0512"/>
    <dbReference type="eggNOG" id="COG5352">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096417_1_0_5"/>
    <dbReference type="OMA" id="EATCHWP"/>
    <dbReference type="OrthoDB" id="2041146at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011681">
        <property type="entry name" value="GcrA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07750">
        <property type="entry name" value="GcrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="168" mass="18515" checksum="350D19121ECD5BDE" modified="2005-10-11" version="1">MTVITWTDDRVEQLKKLWEAGLSASQIAAELGNVTRNAVIGKVHRLGLSGRAKSPSSTVPRTRKARPAQHMVRVSRPVSRGNTALAQAFEVEIEADPIAYDNVVPMSQRRSLLELSEATCHWPVGDPASPEFFFCGGKALTGLPYCAHHSRIAYQPAADRRRQSPKTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVB9</accession>
    <name>Q3SVB9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0505</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0505"/>
    <dbReference type="EnsemblBacteria" id="ABA03772">
        <property type="protein sequence ID" value="ABA03772"/>
        <property type="gene ID" value="Nwi_0505"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0505"/>
    <dbReference type="eggNOG" id="COG5002">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_2_5"/>
    <dbReference type="OMA" id="CMLMTFH"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="187"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="417" mass="45344" checksum="1F634EF6B08B8F24" modified="2005-10-11" version="1">MRRVALILLVAGLALAALVVFGGLQPLGATVAFACIAGAALVPWQVHDPDASREIEFGANPVETDVARALVAGMPDPNVLLDRAGRVIHLNAAAAQLVPALRRNELAQFALRAPEIVTALREAIATREARRATYLNHVPVDRWMELIITPIPVPTAFGGVDYCMLLTFHDQTPLRRVEEMRADFVANASHELRTPLAALSGFIDTLQGPAREDARARERFLGIMHAQAARMARLIDDLLSLSRVELSVHVRPETIVDIVPIVREVVDGLEPLAAERHVEIETALPETPVLICGDREELLRLFENLIENALKYGASGGRVMVSSMLDVSSEGNPEFRVVVRDFGPGIAPEHLPRLTERFYRVDAGDSRAQGGTGLGLSLVKHILNRHRGRLLIESVPGQGASFTAGFPPARDVPEARS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVC7</accession>
    <name>RL32_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L32</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpmF</name>
        <name type="ordered locus">Nwi_0497</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL32 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313825.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVC7"/>
    <dbReference type="SMR" id="Q3SVC7"/>
    <dbReference type="STRING" id="323098.Nwi_0497"/>
    <dbReference type="EnsemblBacteria" id="ABA03764">
        <property type="protein sequence ID" value="ABA03764"/>
        <property type="gene ID" value="Nwi_0497"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0497"/>
    <dbReference type="eggNOG" id="COG0333">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129084_2_2_5"/>
    <dbReference type="OMA" id="GMHRAHD"/>
    <dbReference type="OrthoDB" id="2092354at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00340">
        <property type="entry name" value="Ribosomal_L32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002677">
        <property type="entry name" value="Ribosomal_L32p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044957">
        <property type="entry name" value="Ribosomal_L32p_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35534">
        <property type="entry name" value="PTHR35534"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01783">
        <property type="entry name" value="Ribosomal_L32p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01031">
        <property type="entry name" value="rpmF_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000225743" description="50S ribosomal protein L32">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="13"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="61" mass="6926" checksum="9F44E1755ABAE6DF" modified="2005-10-11" version="1">MAVPRRKTSPSRRGMRRSADAISKPTYAEDKDSGELRRPHHLDLKTGMYKGRQVLKAKSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVG2</accession>
    <name>Q3SVG2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA ligase (ATP)</fullName>
            <ecNumber evidence="1">6.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0462</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.5.1.1" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313793.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0462"/>
    <dbReference type="EnsemblBacteria" id="ABA03729">
        <property type="protein sequence ID" value="ABA03729"/>
        <property type="gene ID" value="Nwi_0462"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0462"/>
    <dbReference type="eggNOG" id="COG1793">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005138_6_2_5"/>
    <dbReference type="OMA" id="WLFEESY"/>
    <dbReference type="OrthoDB" id="1073940at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026333">
        <property type="entry name" value="ATP_dep_DNA_lig_pp_1105_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04120">
        <property type="entry name" value="DNA_lig_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00697">
        <property type="entry name" value="DNA_LIGASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA_LIGASE_A3" evidence="4">
        <location>
            <begin position="349"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="594" mass="67000" checksum="03370C996FD0DCF4" modified="2005-10-11" version="1">MNRFAELLDRLAYEPGRNNKLRLMTAYFRETPDPDRGYALSALTGALSFKYAKSGLVRDLIAARADPVLFAFSYDYVGDLSETVALMWPQQHPPDASSVLSAARGSGTGKATGTGHVRHWPSPTLTDVVTTLRTLGKAELPAQLARWLDQLDETGRWALLKLVTGAMRIGVSARLAKTAVAALGSRDAHDVELIWPGLAPPYPDLFAWLEGRGDKPVNRAPAPFRPVMLAHAIEDADFARLDATDFTAEWKWDGIRVQAVRGRDEHGNVETRLYSRTGEDITHSFPDLLPSLRREGFAEGWLNDSREPVRGFRPRAFAIDGELLVVRDGRVQSFNVLQQRLNRKAVTPRLMKEFPVRLRAYDLLADGDNDLRELPFEDRRRRLVDFIAALDDPRIDLSPQVSFATWSDLAAIRADPAAAGADAEAVEGIMLKRRDAPYMPGRPKGLWWKWKRDPHVIDAVLMYAQRGHGRRSSYYSDYTFGVWTAGEEGERLVPVGKAYFGFTDEELLRIDRFVRRNTTESFGPVRHVLHEPDQGLVLEIAFEGLQRSARHKSGVAMRFPRINRLRWDKPPREADRLETLERMLASEATRSNGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVL0</accession>
    <name>Q3SVL0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_0414</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC-4 integral membrane protein family. LolC/E subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313745.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0414"/>
    <dbReference type="EnsemblBacteria" id="ABA03681">
        <property type="protein sequence ID" value="ABA03681"/>
        <property type="gene ID" value="Nwi_0414"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0414"/>
    <dbReference type="eggNOG" id="COG3127">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009475_3_1_5"/>
    <dbReference type="OMA" id="LIMWRLS"/>
    <dbReference type="OrthoDB" id="127265at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038766">
        <property type="entry name" value="Membrane_comp_ABC_pdt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30287">
        <property type="entry name" value="PTHR30287"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="321"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="364"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="410"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="436"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="481"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="726"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="771"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="814"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="8">
        <location>
            <begin position="300"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="8">
        <location>
            <begin position="759"/>
            <end position="842"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA03681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="851" mass="90062" checksum="74A6380CC58074B8" modified="2005-10-11" version="1">MTAKMTRARFLPLRLALRELRGGLRGFYVFIACIALGVMAIAGVGSVASSLSDGLAREGRTLLGGDVSFSLIQREARPDEIAFLRARGTVSVAATLRAMARAPDGRLALVELKAVDGAYPLLGAPTLKPDIPMPDLLTERDGVFGAAADPTLLTRLDLKIGDRVTIGSATFGIRSAVESEPDKLAGGLGLGPRFLVSIDGLRAAALLQPGSLVRWIYRVRLAGNDAGDRATATLGEAARTALPEAGWEIRSRTNASPQLERTILRFTQYLTLVGLAALLVGGVGVGNAVKSHIDRRRDIIATLKAVGATGRDVFAIYLSQVMVLAAIGSLAGLMIGAALPFIIVGAFGHLLPLPVAPMLHPDELALSAVYGLLTALAFGMWPLTRVHDIPVSALFRDTVAHEVVRPQARYLVLIALLIATLAGIAVGLAYDKRVALVFVAAAAIVFALLRGIASGLMALARRLPRSRITMVRLAVANIHRPGTLTPSVVLSLGLGLAVLVTITQIDGNLRRQFSAALPERAPTLYFIDIPSSVSARFDEFLKRLTPQATVEDVPMLRGRIVAARGVKAEELKPSRDAQWVLQSDRGLTYTGEVPKGSKVVEGEWWGPDYDGPPLVSFEKKLADGLGLKIGDTVNVNVLGRDITATISNMRNVDWQSLGINFVLVFSPHAFRGAPHTHIATLTEMHDSPAKDASIIKAVADAFPMVTSVRVREALETIGTVVTNLVLAIRGASTVTLVSAILVLGGALAAGHRHRVYDAVILKTLGATRLRLLGAYALEYLMIGAATAVFGMAAGSLAAWLIVTRLMTLGFEWQAGSAAIVVAAALIVTVALGLAGTLMALTRKPASVLRNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVL1</accession>
    <name>Q3SVL1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_0413</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313744.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0413"/>
    <dbReference type="EnsemblBacteria" id="ABA03680">
        <property type="protein sequence ID" value="ABA03680"/>
        <property type="gene ID" value="Nwi_0413"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0413"/>
    <dbReference type="eggNOG" id="COG0670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058671_1_1_5"/>
    <dbReference type="OMA" id="SHNAYAE"/>
    <dbReference type="OrthoDB" id="1947455at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006214">
        <property type="entry name" value="Bax_inhibitor_1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291">
        <property type="entry name" value="PTHR23291"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01027">
        <property type="entry name" value="Bax1-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="77"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="236"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA03680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="263" mass="28215" checksum="D0989C7D94F7F22F" modified="2005-10-11" version="1">MSDLDRNYATPLGRADGRVDAAAVDAGLRAYMLKIYNYMSIGLAITGLAALGVYMAAVTGDPSGAAAKLGNAYLTPFGYAMFVSPLKWVFILAPLAMVFVISFGINRLKPATAQMLFWAFSALMGLSLSSIFLVYTHTSIVRVFFITAASFGALSLFGYTTKRDMTGMGSFLFMGLIGVIIASLVNLFIASSALQFVVSVAGVLVFAGLTAWDTQRLKNDYIYGYASQGGEIAERAAITGALSLYLNFINLFTLLLQLLGQRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVL6</accession>
    <name>Q3SVL6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0408</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03675">
        <property type="protein sequence ID" value="ABA03675"/>
        <property type="gene ID" value="Nwi_0408"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0408"/>
    <dbReference type="HOGENOM" id="CLU_2247188_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="104" mass="12036" checksum="7A90AF5C6CD838AD" modified="2005-10-11" version="1">MSNPEWLLWPESVAITLIRADASIRTARDYFDRTKDRMCFTMRRFQGRGLSFGTSPERNAPESLTPDQSGFVHRNISCLTGDMETRSSSEPRDRSRNYIPFMNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVL7</accession>
    <name>Q3SVL7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0407</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0407"/>
    <dbReference type="EnsemblBacteria" id="ABA03674">
        <property type="protein sequence ID" value="ABA03674"/>
        <property type="gene ID" value="Nwi_0407"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0407"/>
    <dbReference type="eggNOG" id="COG3064">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072063_0_0_5"/>
    <dbReference type="OMA" id="MEIWFAK"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021457">
        <property type="entry name" value="DUF3108"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11306">
        <property type="entry name" value="DUF3108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229286" evidence="1">
        <location>
            <begin position="38"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="289" mass="30861" checksum="0AC1DA202800F3AA" modified="2005-10-11" version="1">MSLCRTLLFRRIFCSVWRPAGLSIAILAAMLTAQAQAQGKLEARYDVTLAGILVGTGAWAVDILENQYSAAASGGATGLLKALAHGTGTGSSQGRIVNGALIPASYTATISSSRKSETIRMSLASGNVKGFVIKPSPPVDPKRIPVTDAHKRGVSDPMSATLLLVPDAAELLGRDACHSSTGIFDGRMRYDLRLEFKRMDTVKSGKGYQGPVVVCAIYFSPIAGYVPDRYAIKYLASQRDMEVWLAPIAGTRVLVPYRLTIPTPLGTGKLEATEFNTTATPEQTPVKTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVN7</accession>
    <name>Q3SVN7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NUDIX hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0387</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0387"/>
    <dbReference type="EnsemblBacteria" id="ABA03654">
        <property type="protein sequence ID" value="ABA03654"/>
        <property type="gene ID" value="Nwi_0387"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0387"/>
    <dbReference type="eggNOG" id="COG0494">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037162_19_2_5"/>
    <dbReference type="OMA" id="HADQPGM"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008413">
        <property type="term" value="F:8-oxo-7,8-dihydroguanosine triphosphate pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003561">
        <property type="entry name" value="Mutator_MutT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029119">
        <property type="entry name" value="MutY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14815">
        <property type="entry name" value="NUDIX_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00586">
        <property type="entry name" value="mutt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="4">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="8-oxo-dGTP binding" evidence="2">
        <location>
            <begin position="30"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>8-oxo-dGTP</name>
            <dbReference type="ChEBI" id="CHEBI:77896"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR603561-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR603561-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="129" mass="14525" checksum="E56EC24FEDFAC0EF" modified="2005-10-11" version="1">MVVACALIDADNRVLIAQRPEGKQLAGLWEFPGGKFEPGERPEEALIRELREELGIVTQEGCLAPLTFASHAYESFHLLMPLYICRRWEGTVTSKEGQALKWVRANKLRDYPMPPADIPLIPPLVDLLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVP6</accession>
    <name>Q3SVP6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphoenolpyruvate--protein phosphotransferase</fullName>
            <ecNumber evidence="5">2.7.3.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_0378</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-histidyl-[protein] + phosphoenolpyruvate = N(pros)-phospho-L-histidyl-[protein] + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:23880"/>
            <dbReference type="Rhea" id="RHEA-COMP:9745"/>
            <dbReference type="Rhea" id="RHEA-COMP:9746"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:64837"/>
            <dbReference type="EC" id="2.7.3.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PEP-utilizing enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.7.3.9" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313709.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0378"/>
    <dbReference type="EnsemblBacteria" id="ABA03645">
        <property type="protein sequence ID" value="ABA03645"/>
        <property type="gene ID" value="Nwi_0378"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0378"/>
    <dbReference type="eggNOG" id="COG3605">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007308_7_1_5"/>
    <dbReference type="OMA" id="MFPMISE"/>
    <dbReference type="OrthoDB" id="467393at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008965">
        <property type="term" value="F:phosphoenolpyruvate-protein phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008279">
        <property type="entry name" value="PEP-util_enz_mobile_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000121">
        <property type="entry name" value="PEP_util_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036637">
        <property type="entry name" value="Phosphohistidine_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006318">
        <property type="entry name" value="PTS_EI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008731">
        <property type="entry name" value="PTS_EIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036618">
        <property type="entry name" value="PtsI_HPr-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05524">
        <property type="entry name" value="PEP-utilisers_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00391">
        <property type="entry name" value="PEP-utilizers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02896">
        <property type="entry name" value="PEP-utilizers_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47831">
        <property type="entry name" value="SSF47831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52009">
        <property type="entry name" value="SSF52009"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01417">
        <property type="entry name" value="PTS_I_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0598">Phosphotransferase system</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="GAF" evidence="11">
        <location>
            <begin position="24"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA03645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="755" mass="82370" checksum="846FB8E61EBCE188" modified="2005-10-11" version="1">MRSASSGPRVLLRRLREVMAEPVSAQERLDKIVVLIAANMVAEVCSTYVLRVDNTLELYATEGLNRDAVHRTVLTAHEGLVGLVASEATPLNLSDARSHPAFSFRPETGEEIYNSFLGVPILRAGNTLGVLVVQNRARRTYVEEEVEALQTTAMVLAEMIASGELAALAQPGLEPAARHTIHQIGAILSDGIALGHVVLHEPRIVITNYIADDVPKEIRRLETALTKLRTDLDRLLERGDVAEGGEHRDVLEAYRMFANDHGWSHRLQEAAATGLTAEAAVERVQSDTRARMLRSTDPYLRDRLHDLEDLGHRLMRQLVGQDHAPSREQLPDNAILIARAMGPAALLDYDRRRLRGLVLEEGTATSHVAIVARALGIPAVGEVPNAVGIADPGDAIIVDGTSGSIYLRPSAEIESAYAERVRFRARRQAQYIALRDKPCVTRDGEPVDLMINAGLVIDLPHLEDTNCSGIGLFRTELQFMLGQSLPRASEQLALYRKVLDAAGDKPVTFRTLDIGGDKALPYMEAVAEENPALGWRAIRLGLDRPGLLRGQIRALLRAGSGRALRIMFPMISEVAEFDQAKAIVERELTYLRQHDHTLPERVDVGSMVEVPALLYQLDELFAKADFVSVGSNDLFQFMFAVDRGNSKVSNRFDTLSPPILRALRDIVVKARAAGKSASLCGEMASQPLGALALIALGYRSLSLSATAHGPVKALILDLDTKKAEAAILPLLEAPSGSISVRDKLKDFADAEGLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVQ3</accession>
    <name>Q3SVQ3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0371</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0371"/>
    <dbReference type="EnsemblBacteria" id="ABA03638">
        <property type="protein sequence ID" value="ABA03638"/>
        <property type="gene ID" value="Nwi_0371"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0371"/>
    <dbReference type="eggNOG" id="COG2801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2539145_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="83" mass="9349" checksum="B0E37E7345566977" modified="2005-10-11" version="1">MEGCAMGQVLHGSATTTEAIRRAIQNSEESLRALSKRYGIKHLERFRAKRTPVRVKKTRQIKIIGPASDSIRSGKALERDEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVQ8</accession>
    <name>Q3SVQ8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Integrase, catalytic region</fullName>
            <ecNumber evidence="3">2.7.7.49</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0366</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0366"/>
    <dbReference type="EnsemblBacteria" id="ABA03633">
        <property type="protein sequence ID" value="ABA03633"/>
        <property type="gene ID" value="Nwi_0366"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0366"/>
    <dbReference type="eggNOG" id="COG2801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027402_15_1_5"/>
    <dbReference type="OMA" id="TWIERIM"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="127"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="329" mass="37859" checksum="2CC35F5236CF0584" modified="2005-10-11" version="1">MGQVLHGSATTTEAIRRAIQNSEESLRALSKRYGINQKTVAKWKKRTSVADLPTGPKDPRSTALSSEEEAIIVAFRKHTLLPLDDCLYALQPTIPHLSRSSLHRCLHRHGISRLPEVEGDKPIRKTFRSYPIGYFHIDIAEVQTAEGKLRLFVAIDRTSKFAYAELHQEAGKMVAAQFLRNLIVAVPYAIHTVLTDNGIQFTNHARHKYAFHHIFDRVCDENGIEHRLTRINHPWTNGQVERMNRTIKDATVKRFHYDNHDQLRRHLQDFIKAYNFGRRLKTLKAPHTLRVHLQTMDFRARSIHHRSNPSNAGTEHLGHRLIKSQPDAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVR0</accession>
    <name>Q3SVR0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0364</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0364"/>
    <dbReference type="EnsemblBacteria" id="ABA03631">
        <property type="protein sequence ID" value="ABA03631"/>
        <property type="gene ID" value="Nwi_0364"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0364"/>
    <dbReference type="eggNOG" id="COG3415">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056788_2_3_5"/>
    <dbReference type="OMA" id="RSVWAFV"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001523">
        <property type="entry name" value="Paired_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00292">
        <property type="entry name" value="PAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0563">Paired box</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Paired" evidence="2">
        <location>
            <begin position="2"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="116" mass="13165" checksum="ECA20834D1F591E0" modified="2005-10-11" version="1">MGRAYSNDLRERVVGAVVKGGLSRHQAAAQFGVGISTAINWVQRFHETGSVAPSQIGGYRPKKIAGPHREWLLQRCRKDFTVRGLVAELAERGLKVDYRTMWEFVHAVKLSYKKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVT2</accession>
    <name>Q3SVT2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0342</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313674.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0342"/>
    <dbReference type="EnsemblBacteria" id="ABA03609">
        <property type="protein sequence ID" value="ABA03609"/>
        <property type="gene ID" value="Nwi_0342"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0342"/>
    <dbReference type="eggNOG" id="ENOG5032ZH9">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154487_0_0_5"/>
    <dbReference type="OMA" id="WATVNKD"/>
    <dbReference type="OrthoDB" id="2073466at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="112" mass="12011" checksum="6E3B0AFD9F8AB215" modified="2005-10-11" version="1">MPRGDKSAYTGKQKRKAEHIEEGYEKRGVKKEEAERRAWATVNKESGGGKKSGSGRGRKTTNVSSKRGGKLGGKASAARSAADRSRAAKKGAATRKRNAVKRSPTRKAAGKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW25</accession>
    <name>Y248_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0262 protein Nwi_0248</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">Nwi_0248</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0262 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313582.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SW25"/>
    <dbReference type="STRING" id="323098.Nwi_0248"/>
    <dbReference type="EnsemblBacteria" id="ABA03516">
        <property type="protein sequence ID" value="ABA03516"/>
        <property type="gene ID" value="Nwi_0248"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0248"/>
    <dbReference type="eggNOG" id="COG5328">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112904_0_0_5"/>
    <dbReference type="OMA" id="TLICVLH"/>
    <dbReference type="OrthoDB" id="1859749at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00678">
        <property type="entry name" value="UPF0262"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008321">
        <property type="entry name" value="UCP032146"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06793">
        <property type="entry name" value="UPF0262"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032146">
        <property type="entry name" value="UCP032146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000314201" description="UPF0262 protein Nwi_0248">
        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00678"/>
        </source>
    </evidence>
    <sequence length="167" mass="18919" checksum="F112A5FEA837D7E6" modified="2005-10-11" version="1">MSKETPSEDDSRHRIVAVTLDEDSIGRSGPDIEHERAIAIYDLVEKNLFAPEGAEGGPFTLHIAITGNRLMFDIRREDGAPVVTHLLSLTPFRRIVKDYFMICDSYYQAIRTATPDKIEAIDMGRRGIHDEGSRTLQERLNGKVHVDFETARRLFTLISVLHWKGNA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW64</accession>
    <name>AROA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-phosphoshikimate 1-carboxyvinyltransferase</fullName>
            <ecNumber evidence="1">2.5.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5-enolpyruvylshikimate-3-phosphate synthase</fullName>
            <shortName evidence="1">EPSP synthase</shortName>
            <shortName evidence="1">EPSPS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aroA</name>
        <name type="ordered locus">Nwi_0209</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1-carboxyvinyl)-3-phosphoshikimate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21256"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="EC" id="2.5.1.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the EPSP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.19" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SW64"/>
    <dbReference type="SMR" id="Q3SW64"/>
    <dbReference type="STRING" id="323098.Nwi_0209"/>
    <dbReference type="EnsemblBacteria" id="ABA03477">
        <property type="protein sequence ID" value="ABA03477"/>
        <property type="gene ID" value="Nwi_0209"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0209"/>
    <dbReference type="eggNOG" id="COG0128">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024321_0_1_5"/>
    <dbReference type="OMA" id="YEDHRMA"/>
    <dbReference type="OrthoDB" id="533829at2"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00089"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003866">
        <property type="term" value="F:3-phosphoshikimate 1-carboxyvinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01556">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00210">
        <property type="entry name" value="EPSP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001986">
        <property type="entry name" value="Enolpyruvate_Tfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036968">
        <property type="entry name" value="Enolpyruvate_Tfrase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006264">
        <property type="entry name" value="EPSP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023193">
        <property type="entry name" value="EPSP_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00275">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000505">
        <property type="entry name" value="EPSPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="SSF55205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01356">
        <property type="entry name" value="aroA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00104">
        <property type="entry name" value="EPSP_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000058604" description="3-phosphoshikimate 1-carboxyvinyltransferase">
        <location>
            <begin position="1"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphoenolpyruvate" evidence="1">
        <location>
            <begin position="103"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="332"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="360"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="28"/>
            <end position="29"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="359"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="406"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00210"/>
        </source>
    </evidence>
    <sequence length="449" mass="46982" checksum="2596733D535D108C" modified="2005-10-11" version="1">MTLSGQTMPLEARKCQSLTGRVRVPGDKSISHRALILGALAVGETRISGLLEGEDVLNTAKAMQALGAKVERRTDDNGGIAWSVRGVGTGGFATPEAPLDFGNSGTGCRLVMGAVAGCPIRAAFDGDGSLRSRPMRRILDPLELMGARVISQSDGGRLPLTLEGARDPLPIVYRTPVASAQIKSAVLLAGLSAPGVTTVIEREASRDHTELMLKHFGADIVSVAEGAHGRKISLTGQPELHGAGVTVPADPSSAAFPIVAALITEGSDIVLDDVMTNPLRTGLFVTLREMGASIEESETRLDAGEPMAQLRVKASRLRGIEVPAERAPSMIDEYLVLAVAAAFAEGTTVMRGLRELRVKESDRLEAAAAMLRAGGVAVEIAGDDLIVEGRGRVPGGGLVTTHMDHRIAMSALVMGCASDAPVKVDDIAFIATSFPDFVPMMQRLGANFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW73</accession>
    <name>Q3SW73_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADPH-dependent FMN reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0200"/>
    <dbReference type="EnsemblBacteria" id="ABA03468">
        <property type="protein sequence ID" value="ABA03468"/>
        <property type="gene ID" value="Nwi_0200"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0200"/>
    <dbReference type="eggNOG" id="COG0431">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055322_4_1_5"/>
    <dbReference type="OMA" id="FAGIRCI"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN_red" evidence="1">
        <location>
            <begin position="9"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="197" mass="21147" checksum="84183D5EF93B3078" modified="2005-10-11" version="1">MRSTGLFAPKILIIPGSLRTGSQNARLAAVIAKEFIRADVEVTRISLGDFPLPIYDADLQASAGVPASAVNLKQMIAAHHGVVIVTPEYNASLPPLLKNAIDWISRVQSPHEARGQVFHERAFAIASASTGRFGGARCLAQLRLILSVCGAMVIPNQLALSFAGDAYDDMERLKRSADADALKKMVRQLITLAGNMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW90</accession>
    <name>Q3SW90_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sel1-like repeat protein</fullName>
            <ecNumber evidence="2">3.5.2.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0183</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.2.6" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313519.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0183"/>
    <dbReference type="EnsemblBacteria" id="ABA03451">
        <property type="protein sequence ID" value="ABA03451"/>
        <property type="gene ID" value="Nwi_0183"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0183"/>
    <dbReference type="eggNOG" id="COG0790">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_278601_0_0_5"/>
    <dbReference type="OMA" id="ITEAMPR"/>
    <dbReference type="OrthoDB" id="156533at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008800">
        <property type="term" value="F:beta-lactamase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="524"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="582"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="727"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1017"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="598"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="746"/>
            <end position="760"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1059" mass="111677" checksum="57807A6C07F12DF3" modified="2005-10-11" version="1">MNSRGSSSDDGIETSDREGADAAARRAGTSRDDGPGSGSGGTPPGPTQPAASPQNSPEMRDIHQRLDSITRQIDQLASPAENGEPAVARQLNDAISRLDARLARVTAQTPAANPQQRGASGSYDRPTSPDLVTLESAIAEIAARQHELDETPRAPSSYSSAPVASAMRTSRQADFSSLERQLFKITSQIESLQRPDGIEQSIAAFRIELADIRHVITEAMPRRAIESLENEIRSLSQRIDEVRQNGSDGQALANIERALKEIYDALRSLKPAEQLSGFDEAIRNLGNKIDSIVRGSGDSGMMQQLESAIGALRGIVSNVASNDALERLGNDLNMLSSKVEQLGRPAGDGDFYAALEQRMAALTQTLENRESPASGSSFEQLEETVRALSERLDRLPAGHDSSSALAHLEQRVSMLLERLETAGEYSGSHLGRVEEGLQDILHCLERQQAGLAAMSESGPRSAVPTMDSEVVEAIKRELSEMRFCQSETDRHTQDSLEAVHNTLEHVVDRLAMIESDLRAVHTMPARAEPSRGGIMPERTANLPPKPELPNPVLSQAATQPTPARTATAAPIPRAIADALIPKETFDPDRVVPSTTAPSPRPAIDPKLPPDHPLEPGTRPAGRAATPSERIAASESETGDVAETPREQSGSSFIAAARRAAKAAAASTPSPDKAGRTKVAIEPARPATGGSDITSKIRSLLVAASVVVIVLSSFKFAMTLLDSSPRATLSESDHAAPVTKPPADPEGSAGPEIPQPPSMIAPTPIDRQSMIAPPAGDSAAPAKNPSDAMPASRPDTPSADVTGAIPNGLTPEPAATPPAASLPDSIGGTTLRSAALQGDPAASFEVGVRYAEGKGVTVNYDEAAKWYERAAHAGIVPAMFRLGALHEKGLGTSKDVDTARRYYLQAADRGNAKAMHNLAVLDADGGGKGADYVSAAQWFSKAAERGIADSQYNLGILYARGIGVEQNLAKSYKWFSLAAAQGDVDSGRKRDEVAKRLDPPSLAAAKLAVQTFVVTPQPSDAVSVPGPAGGWDSAAAKPRAKPAPGKSARANHAMARHTAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW97</accession>
    <name>Q3SW97_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0176</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313513.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0176"/>
    <dbReference type="EnsemblBacteria" id="ABA03444">
        <property type="protein sequence ID" value="ABA03444"/>
        <property type="gene ID" value="Nwi_0176"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0176"/>
    <dbReference type="eggNOG" id="ENOG5033N97">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1923588_0_0_5"/>
    <dbReference type="OMA" id="WGFRRSR"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229349" evidence="3">
        <location>
            <begin position="21"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="30"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="126" mass="12773" checksum="1D0474D35342DD62" modified="2005-10-11" version="1">MKTVIAVVASLALLSSVAMAQERAGSAALGALSGAVVLGPVGAVAGAVVGYTAGPSIARSWGLRRSSARRRQSSRPSHRRTTANGSRRTPDAQTASRNAAPAAATAAEPHPVHPRPSMPPVQALDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWA5</accession>
    <name>Q3SWA5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0168</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313505.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0168"/>
    <dbReference type="EnsemblBacteria" id="ABA03436">
        <property type="protein sequence ID" value="ABA03436"/>
        <property type="gene ID" value="Nwi_0168"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0168"/>
    <dbReference type="HOGENOM" id="CLU_135588_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="119" mass="12948" checksum="D7BAFA9427735873" modified="2005-10-11" version="1">MQADVKKQMLSILNFRLLALLVPALALSILAGMVMYALGYFSMVYGEVVLVLKILMHLLLAVWSALKAILLGTVVFGLLLVFLSPVLIVLWELRKTRNLPSDDAILPPQTSSGVAGETP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWD4</accession>
    <name>Q3SWD4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">O-succinylhomoserine sulfhydrylase</fullName>
            <shortName evidence="2">OSH sulfhydrylase</shortName>
            <shortName evidence="2">OSHS sulfhydrylase</shortName>
            <ecNumber evidence="2">2.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">metZ</name>
        <name evidence="5" type="ordered locus">Nwi_0139</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the formation of L-homocysteine from O-succinyl-L-homoserine (OSHS) and hydrogen sulfide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>hydrogen sulfide + O-succinyl-L-homoserine = L-homocysteine + succinate</text>
            <dbReference type="Rhea" id="RHEA:27826"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57661"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 2 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homocysteine from O-succinyl-L-homoserine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the trans-sulfuration enzymes family. MetZ subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313476.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0139"/>
    <dbReference type="EnsemblBacteria" id="ABA03407">
        <property type="protein sequence ID" value="ABA03407"/>
        <property type="gene ID" value="Nwi_0139"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0139"/>
    <dbReference type="eggNOG" id="COG0626">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018986_2_0_5"/>
    <dbReference type="OMA" id="THGGIIV"/>
    <dbReference type="OrthoDB" id="637281at2"/>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00449"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071266">
        <property type="term" value="P:'de novo' L-methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071268">
        <property type="term" value="P:homocysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02056">
        <property type="entry name" value="MetZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006234">
        <property type="entry name" value="O-succ-hSer_sulfhydrylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="PTHR11808"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01325">
        <property type="entry name" value="O_suc_HS_sulf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="2 3 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2 3">
        <location>
            <position position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="397" mass="42891" checksum="B84D7E90DC09A242" modified="2005-10-11" version="1">MSETKSAPRYHPETRLVHSGTLRSQFAETSEALFLTQGYVYDTAEQCEARFKGDDPGFIYSRYSNPTVAMFEQRMAALEGAETARALATGMAAVTTAILAPLKAGDHVVAAKALFGSCRYVVEDLLPRYGIESTLVDGLDLDQWRKAMRPNTKTCFIESPTNPTLDVLDIGGIAEIAHQAGARLIVDNVFATPIWQSPLALGADVVVYSATKHIDGQGRTLGGVILSSNAFVQEHLQTFIRQTGPSISPFNAWILLKGLETLGVRVRQQTDSAAAIAEVLASHPKVSRLVYPGRPDHPQAALVKKQMRAGSTLIGFEVKGGKAAAFRCLNALKIARISNNLGDAKSLVTHPATTTHQRLAPEIRAGLGIGESFIRYSVGLEHKDDLIEDLVTALEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWG3</accession>
    <name>Q3SWG3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_0110</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313447.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0110"/>
    <dbReference type="EnsemblBacteria" id="ABA03378">
        <property type="protein sequence ID" value="ABA03378"/>
        <property type="gene ID" value="Nwi_0110"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0110"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046130_1_1_5"/>
    <dbReference type="OMA" id="LNRWHLM"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="105"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="5">
        <location>
            <begin position="216"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA03378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="446" mass="48626" checksum="3F21BD7BD2D25FDB" modified="2005-10-11" version="1">MPDVAASDFRHPRRYVRLDTILRLRWLAALGQLAAIFVVAQGLQFDLPIIPCVVIVGLSSLLNLVLQVSFNPMQRLEPRHAAALLALNIAELAVLLFFTGGLQNPFSYLFLAPVLISATALPTRMTMALGGFAVACATVLGFTHLPLPWDASNPLILPPIYLIGVWLSIVLAIGVTSLYTFQVTEQARKLSDALAATELVLTREQHLTQIDGLAAAAAHELGTPLSTIFLISRELEKALQENGAQEPQRLAGDLRIVREQAQRCRDILAKIAQLSSSGAPFDRMPLSTLIEETVAPHRDFGIDIKTRIEAAGDHEPVGIRNPAILYGVGNILENAVDFARSAVEVNAWWSKDSVEIVIADDGPGIVPDILKRIGEPYLSRRRNVGDAQSGRTGLGLGIFIARTLLERTGARVSFKNRTFPDHGAVVRIVWPRIRFETAKTIAETTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWK1</accession>
    <name>Q3SWK1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Methyl-accepting chemotaxis sensory transducer with Pas/Pac sensor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0072</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313409.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0072"/>
    <dbReference type="EnsemblBacteria" id="ABA03340">
        <property type="protein sequence ID" value="ABA03340"/>
        <property type="gene ID" value="Nwi_0072"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0072"/>
    <dbReference type="eggNOG" id="COG2202">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_26_5"/>
    <dbReference type="OMA" id="IWIQAAY"/>
    <dbReference type="OrthoDB" id="429006at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAC" evidence="2">
        <location>
            <begin position="85"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="2">
        <location>
            <begin position="207"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="2">
        <location>
            <begin position="330"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="513"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="514"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="567" mass="62395" checksum="902CD8B005AF3F41" modified="2005-10-11" version="1">MFFFKKWSIAKEDAAKTAAIRKSQAVIELGLDGTVVTANENYLSIFGYTLADVRGQHHSMFVEADERSGAAYRDFWARLNKGETQSAEFKRIDRHGDEVWVQASYIPVLDSRGNPKKIIALAADITAKKIKELENAGQVSAIERAQAVVEFNLDGTIITANKNFLNVMGYTLGEMQGKHDRVFVESSERAGDAYRDFWARLNLGEPQSAEYKRINKAGNEVWLLATFNPIVDESGKPFKVVAFATDVTKQKVKSADLEGQITAIGKSQAVVELGMDGKILTANDNFLKTFGYALLKDIQGKHHNMFVEAAERTSTAYRDFWARLARGEAQSAEYKRIGKDGKTVWLQASYNPVLDSRGKPVKVVEIATDITARKLKALENEGQLAAIERAQAIASFRPDGTIIAANDKYLKAMGYTLGEVSGKHDGMFVESSERSGSAYREFWGKLNRGEAQSAEFKRVAKDGSEVRIRASYIPVLDAQGKTTKVVQYAMVIESAAAASEEMSAPIQEAPVSMAKQADEQSLSMSRLESRRKSVNAGPSPLAQFLTNLREGFSSAVASSTRAVANFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWK9</accession>
    <name>Q3SWK9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Diphthami_syn_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313403.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0064"/>
    <dbReference type="EnsemblBacteria" id="ABA03332">
        <property type="protein sequence ID" value="ABA03332"/>
        <property type="gene ID" value="Nwi_0064"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0064"/>
    <dbReference type="eggNOG" id="COG2102">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010289_1_1_5"/>
    <dbReference type="OMA" id="DMFCNGI"/>
    <dbReference type="OrthoDB" id="1144452at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01994">
        <property type="entry name" value="Alpha_ANH_like_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002761">
        <property type="entry name" value="Diphthami_syn_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01902">
        <property type="entry name" value="Diphthami_syn_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Diphthami_syn_2" evidence="1">
        <location>
            <begin position="5"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="222" mass="24179" checksum="5BDBD4665EB8CF38" modified="2005-10-11" version="1">MTRPKALISWSSGKDSAFALHEIRREGAFDVVGALTTVNERFGRVSIHGVRQELLDAQLTAAGLSPRVVPIPDPCPNDAYEARMTRTMADAKRSGITHIIFGDLFLADIRAYREQKLAGAGITPVFPLWGRPTSTLAREMIASGLEARLVSVDLAKLDRSLAGRDFDERLLEDLPAGIDPCGENGEFHTFVSAAPVFSQRLEVLCGDVVERGGFAYRDLVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWN5</accession>
    <name>Q3SWN5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UBA/THIF-type NAD/FAD binding fold protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0038</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0038"/>
    <dbReference type="EnsemblBacteria" id="ABA03306">
        <property type="protein sequence ID" value="ABA03306"/>
        <property type="gene ID" value="Nwi_0038"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0038"/>
    <dbReference type="eggNOG" id="COG0476">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013325_10_0_5"/>
    <dbReference type="OMA" id="EVQIHAF"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008641">
        <property type="term" value="F:ubiquitin-like modifier activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="PTHR10953"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="SSF69572"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ThiF" evidence="1">
        <location>
            <begin position="22"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="278" mass="29709" checksum="29B2B116D9DD44DD" modified="2005-10-11" version="1">MRRLIDGENGRTMLSADELERYSRHIVMREVGGPGQNALRNARVLMIGAGGLGAPALMYLAAAGVGTLGVIDDDVVTLSNLQRQIIHATPDIGSPKVESAADKIRALNPHVAFRAHHMRLDASNAMALLADYDIVAEGSDNFATRYLVADACFLAKKPLVTAALGVFDGSLTTIRAHEKNADGVFNPTYRCLFPEPPPEGTVPSCQQAGILGALAGVLGSMTALEVIREIVGFGEGLVGRLLMVDARAMRFETLNYRRDPSNPLNGDHPTITDLSGYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMY7</accession>
    <name>Q3SMY7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_3107</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06354">
        <property type="protein sequence ID" value="ABA06354"/>
        <property type="gene ID" value="Nwi_3107"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3107"/>
    <dbReference type="eggNOG" id="ENOG50319UD">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2452656_0_0_5"/>
    <dbReference type="OMA" id="FIREFHR"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="75" mass="8622" checksum="E7E947DD5FCB18F7" modified="2005-10-11" version="1">MYAMVYPIHLQRSSERRWASRVAREIPIRRSRPEGTDTCACGNAVTAPVNSQYSPHVVTNEWHCSKCNTRWTTEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN03</accession>
    <name>Q3SN03_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_3091</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3091"/>
    <dbReference type="EnsemblBacteria" id="ABA06338">
        <property type="protein sequence ID" value="ABA06338"/>
        <property type="gene ID" value="Nwi_3091"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3091"/>
    <dbReference type="eggNOG" id="COG4430">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170393_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="120" mass="13914" checksum="69C3002948D627F5" modified="2005-10-11" version="1">MHETRNSSAGRIVGGCMANTFPHGTVHKAGKDLQAALRSDSKVFALWENLTPLGRNEFICWVEDAKQPKTRQRRIERTRDELLEGKKRPCCWVGCIHRTDKMRSRWQQAVLIDQKGKKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN33</accession>
    <name>Q3SN33_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Acetyl-CoA acetyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_3060</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.9" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316224.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3060"/>
    <dbReference type="EnsemblBacteria" id="ABA06308">
        <property type="protein sequence ID" value="ABA06308"/>
        <property type="gene ID" value="Nwi_3060"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3060"/>
    <dbReference type="eggNOG" id="COG0183">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031026_0_0_5"/>
    <dbReference type="OMA" id="ICPSIAI"/>
    <dbReference type="OrthoDB" id="1058688at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003985">
        <property type="term" value="F:acetyl-CoA C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020615">
        <property type="entry name" value="Thiolase_acyl_enz_int_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020610">
        <property type="entry name" value="Thiolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00098">
        <property type="entry name" value="THIOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00099">
        <property type="entry name" value="THIOLASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase_N" evidence="5">
        <location>
            <begin position="5"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase_C" evidence="6">
        <location>
            <begin position="270"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="3">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="348"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA06308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="392" mass="40442" checksum="2BFA2B215268EAD4" modified="2005-10-11" version="1">MSDDVVIVSAARTPVGSFNGALATTPAHALGAIAIKAALERAGVEPGRVSEVIMGQILTAAQGQNPARQASIGAGVPVESPAWGVNQLCGSGLRTVALGYQAILNGDSEIVVAGGQESMSMAPHAQHLRGGVKMGNLELIDTMIRDGLWDAFNGYHMGNTAENVARQYQITRQQQDEFAAASQNKAEVAQKAGRFKDEIVPVTVKNRKGDIVVDTDEYPKAGVTADSIAKLRPAFEKDGTVTAANASGINDGAAAVVLMSAAQASKEGKTPLARIVSWAHAGVDPKIMGTGPIPASRAALKKAGWAIGDLDLIEANEAFAAQACAVNKDLGWDTSRVNVNGGAIALGHPVGASGARVLVTLLHEMQKRDARKGLATLCIGGGMGIAMCVERG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN56</accession>
    <name>Q3SN56_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_3035</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06285">
        <property type="protein sequence ID" value="ABA06285"/>
        <property type="gene ID" value="Nwi_3035"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3035"/>
    <dbReference type="eggNOG" id="ENOG5033PM1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_474644_0_0_5"/>
    <dbReference type="OMA" id="IRHRSAY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="582" mass="64736" checksum="A2D173C0E93284C4" modified="2005-10-11" version="1">MARKEIMTARHLAGAVYRALTNRKDGPTLYEVCDPVLLRHRGGDGHLAKFYKTALANPALRPLLRRAGLPELRDPTRFQELQDALRQARDEETPDWAAIGRPVAALLDTVTLHHPRPGATTCSGMEPGLADIERVIRTCGAHLLRSFRNNGFIPTFAAFNLIGDPDLRGRDFLVALTGLDARGYKNSTLLFNLARVFIARSPAREVINPPWRGVAEPMWEPVQIRHRSAYYDAFFTEALLSFAETGLATPAETDAIRRANCEMVDFCLRTSREEVFSRDGSRVGVITALAPNPHPRFNRFFTHIKQNLGFGIYVPDCDTTACSFSAATQAGSNDPILDQPLLDFYAGYQVGGGANEPLVTVPLNDNIDYDGGIVTWIDSLAGDRPYGNDLDPTLNLDVLEVSFRNCSRWRVLQTPQRLQTVQRIIRFQRRLVESGAFTDPRSHIYYLPELYCAYFGRCYAAFSMLPSAARRAIDPDDSFSYMRGRILGYVQDTLISAEMNVFDAALALIALGHLGADRAALWPALNCIVNHAGEGGRRSPFRAYEWNKMKTPTRIVVGGPEVTSAFVLMGLALARKAMQRVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN73</accession>
    <name>Q3SN73_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Isovaleryl-CoA dehydrogenase</fullName>
            <ecNumber evidence="12">1.3.8.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_3018</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7 8">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.4" evidence="12"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3018"/>
    <dbReference type="EnsemblBacteria" id="ABA06268">
        <property type="protein sequence ID" value="ABA06268"/>
        <property type="gene ID" value="Nwi_3018"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3018"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_1_5"/>
    <dbReference type="OMA" id="VHRWAQE"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008470">
        <property type="term" value="F:isovaleryl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901565">
        <property type="term" value="P:organonitrogen compound catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd01156">
        <property type="entry name" value="IVD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034183">
        <property type="entry name" value="IVD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 8" id="KW-0274">FAD</keyword>
    <keyword evidence="3 8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="11">
        <location>
            <begin position="32"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="10">
        <location>
            <begin position="147"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="9">
        <location>
            <begin position="254"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="265"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="388"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="148"/>
            <end position="157"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="181"/>
            <end position="183"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="361"/>
            <end position="365"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="390"/>
            <end position="392"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR634183-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR634183-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR634183-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA06268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="407" mass="44172" checksum="A8EEE048396D2CC5" modified="2005-10-11" version="1">MVVSAQNRDWLWLHLGEFRVSISNAFNFDLGETADAIRETVRDFAANEIAPRAAEIDTSNQFPRDLWPRLGALGLHGITVEEDYGGAGLGYLEHCIAMEEISRGSASVGLSYGAHSNLCINQLRRNGSDAQKRKYLPKLISGENVGALAMSEPGAGSDVISMRTRADRKGDRYILNGSKMWITNGTIADTLVIYAKTDAAAGARGITAFILEKGMNGFSTAQKLDKLGMRGSDTCELVFEDCEIPEDNVLGDAGRGVNVLMSGLDYERAVLAAGPLGIMQACMDMVMPYVRERKQFGQPIGSFQLVQAKVADMYVTMNACRAYVYAVAKACDRGEITREDAAGAILYSAEKATQCALDAIQLLGGNGYINDYPAGRLLRDAKLYEIGAGTSEIRRMLIGRELFEKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN79</accession>
    <name>Q3SN79_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative serinethreonine protein phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_3012</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3012"/>
    <dbReference type="EnsemblBacteria" id="ABA06262">
        <property type="protein sequence ID" value="ABA06262"/>
        <property type="gene ID" value="Nwi_3012"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3012"/>
    <dbReference type="eggNOG" id="COG0639">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2274396_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="102" mass="11010" checksum="5966DFB43C862182" modified="2005-10-11" version="1">MHIDAHRIYVHAGLDRGIARDAQSEITLLWKRYPKGDASGFEGRHVVHGHNSVAQGPELYQGRTNLDTGAWRTGRLVTGVFDDATPGGPVDFIVVHGPAQGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNA1</accession>
    <name>Q3SNA1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glycerol-3-phosphate dehydrogenase</fullName>
            <ecNumber evidence="6">1.1.5.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_2990</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a quinone + sn-glycerol 3-phosphate = a quinol + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:18977"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.1.5.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.5.3" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2990"/>
    <dbReference type="EnsemblBacteria" id="ABA06240">
        <property type="protein sequence ID" value="ABA06240"/>
        <property type="gene ID" value="Nwi_2990"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2990"/>
    <dbReference type="eggNOG" id="COG0578">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015740_5_0_5"/>
    <dbReference type="OMA" id="VYKGGPH"/>
    <dbReference type="OrthoDB" id="360065at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009331">
        <property type="term" value="C:glycerol-3-phosphate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052591">
        <property type="term" value="F:sn-glycerol-3-phosphate:ubiquinone-8 oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006072">
        <property type="term" value="P:glycerol-3-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.870">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031656">
        <property type="entry name" value="DAO_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038299">
        <property type="entry name" value="DAO_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000447">
        <property type="entry name" value="G3P_DH_FAD-dep"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11985">
        <property type="entry name" value="PTHR11985"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16901">
        <property type="entry name" value="DAO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01001">
        <property type="entry name" value="FADG3PDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00977">
        <property type="entry name" value="FAD_G3PDH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 6 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAO" evidence="7">
        <location>
            <begin position="19"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="DAO_C" evidence="8">
        <location>
            <begin position="400"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA06240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="524" mass="57983" checksum="9534CCDCE58EDA1D" modified="2005-10-11" version="1">MTLSYKMMEEGAPVGQVYDLAIIGGGINGCGIARDAAGRGNSVFLCEMNDLASGTSSGSTKLVHGGLRYLEYYEFRLVREALIEREILWRIAPHIIHPARFVLPHHAGLRPAWLLRFGLFLYDHIGGRKLLPPTRSVDLARDRVGKPLSPGRYHKGFEYSDCVVDDARLVILTARDAADRGADIRTRTRAVETRPSDGFWRVTVEDTRSGERSTIAARVLVNAGGPWIEQVLRLGAGVNSRARVRLVQGSHIVVGKLYDHDRAYIFQNSDGRIVFAIPYQDDFTLIGTTDRDYEGDPARVKASDEEIAYLCASVSEYLAKQVAPRDVVWSYAGVRPLYDDGASEAKAATREYAFELDTPEGAPLLSVYGGKITTYRRLAEEALEKLSPYLKGTKAREGWTSKAALPGGDMEVSAVEGIPADLMRGYPFLTLAHARRLARAYGSRAAAMLGTAKTMADLGREFGATLTEREVRYLMTNEWACTAEDVIWRRSKLGLRMSVADVAALETWFAEQTEGRLREAEARP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNA8</accession>
    <name>Q3SNA8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2983</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316155.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2983"/>
    <dbReference type="EnsemblBacteria" id="ABA06233">
        <property type="protein sequence ID" value="ABA06233"/>
        <property type="gene ID" value="Nwi_2983"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2983"/>
    <dbReference type="eggNOG" id="ENOG502ZAEF">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098257_0_0_5"/>
    <dbReference type="OMA" id="PRLWIDV"/>
    <dbReference type="OrthoDB" id="1846460at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="184" mass="20630" checksum="1E0D557A9C0B548E" modified="2005-10-11" version="1">MSDSPIIGRGVAETAKPRRLRDALWQARIEAADRTGVVVDLRDAEAARLEILNEALDPLFAQVPDQIDLFDRGISKGDTPRLWIDIVAHVVMGRDKRLYRFVQDTRYGRIVLTESHDAAAIVKAVTDYVARRMIERERALSATPLWEPAETRKPRRGYLTFAFGFIAGVAVLFGFALLAALRTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNG4</accession>
    <name>Q3SNG4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">CbbX like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2927</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CbxX/CfxQ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316102.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2927"/>
    <dbReference type="EnsemblBacteria" id="ABA06177">
        <property type="protein sequence ID" value="ABA06177"/>
        <property type="gene ID" value="Nwi_2927"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2927"/>
    <dbReference type="eggNOG" id="COG0464">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008749_1_0_5"/>
    <dbReference type="OMA" id="HRPDNER"/>
    <dbReference type="OrthoDB" id="1115436at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041627">
        <property type="entry name" value="AAA_lid_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000470">
        <property type="entry name" value="CbxX/CfqX_mono"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000641">
        <property type="entry name" value="CbxX/CfxQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17866">
        <property type="entry name" value="AAA_lid_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00819">
        <property type="entry name" value="CBXCFQXSUPER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00820">
        <property type="entry name" value="CBXXCFQX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02880">
        <property type="entry name" value="cbbX_cfxQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="4">
        <location>
            <begin position="60"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA06177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="304" mass="34730" checksum="BC9B30DF9A22A897" modified="2005-10-11" version="1">MQEQVNLREELEAVGIGEILKQLDQELIGLKPVKTRIQEIASLLLVERIRRKMELTSETPTLHMSFTGNPGTGKTTVALRMADILHRLGYVRRGHVVSVTRDELVGQYIGHTAPKTKEILKKAMGGVLFIDEAYYLHRPDNERDYGQEAIEILLQVMESQREDLVVILAGYADRMERFFSSNPGFRSRIAHHIDFPDYSNEELLAIAEVMLRHMNYKFTSDARDAFVRYIALRKAQPLFSNARSIRNALDRIRLRQANRLVADEDAILSVDDVQSVVASDVLASRVFETSQPAVVEQLTDKRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNH6</accession>
    <name>Q3SNH6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved hypothetical membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2915</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316095.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2915"/>
    <dbReference type="EnsemblBacteria" id="ABA06165">
        <property type="protein sequence ID" value="ABA06165"/>
        <property type="gene ID" value="Nwi_2915"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2915"/>
    <dbReference type="eggNOG" id="COG4244">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107155_2_0_5"/>
    <dbReference type="OMA" id="SYRHKIG"/>
    <dbReference type="OrthoDB" id="1566648at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019251">
        <property type="entry name" value="DUF2231_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016923">
        <property type="entry name" value="UCP029509"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09990">
        <property type="entry name" value="DUF2231"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029509">
        <property type="entry name" value="UCP029509"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2231" evidence="2">
        <location>
            <begin position="46"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="154" mass="16496" checksum="CC49F76A72461914" modified="2005-10-11" version="1">MATNPRSTAQVAGHPIHPMVITFPIACFVLAFASDLTFYATSNEFWATASFWLLGIGLVTAALAAGTGLVDVFGDGRVRNLSDTRVHAIGNAIALLIALYNWYMRFEQGTSAVVPSGVVLSGIVVFVLAIAGWKGGEMVFRHRVGVADPRDERD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNK8</accession>
    <name>Q3SNK8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fec I like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2883</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316063.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2883"/>
    <dbReference type="EnsemblBacteria" id="ABA06133">
        <property type="protein sequence ID" value="ABA06133"/>
        <property type="gene ID" value="Nwi_2883"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2883"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_12_3_5"/>
    <dbReference type="OMA" id="LMIDQFR"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="2">
        <location>
            <begin position="15"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="3">
        <location>
            <begin position="112"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA06133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="172" mass="19835" checksum="41395B3D11C41C37" modified="2005-10-11" version="1">MLGTLVQELQQTEEGRLRRFFQRRFRNRADAADATQETFLRLLASAQRSLIENPQAYLFRTARNIAVDQERLQQRRAQVECPITDEKAVLNIPCDAPSPEMALIDKERLHLFEQALIGLPERPRKVLLLRRIEGWSYPAIAQHLGVSPNTVYNDVRLAMGHCMTAMERPKRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNK9</accession>
    <name>Q3SNK9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">FecR protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2882</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316062.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2882"/>
    <dbReference type="EnsemblBacteria" id="ABA06132">
        <property type="protein sequence ID" value="ABA06132"/>
        <property type="gene ID" value="Nwi_2882"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2882"/>
    <dbReference type="eggNOG" id="COG3712">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050192_0_1_5"/>
    <dbReference type="OMA" id="WALCLGE"/>
    <dbReference type="OrthoDB" id="1381035at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006860">
        <property type="entry name" value="FecR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032623">
        <property type="entry name" value="FecR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012373">
        <property type="entry name" value="Ferrdict_sens_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30273">
        <property type="entry name" value="PTHR30273"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16220">
        <property type="entry name" value="DUF4880"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04773">
        <property type="entry name" value="FecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018266">
        <property type="entry name" value="FecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4880" evidence="3">
        <location>
            <begin position="26"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="FecR" evidence="2">
        <location>
            <begin position="128"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA06132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="334" mass="35665" checksum="30CB1AA535DDC62D" modified="2005-10-11" version="1">MTQDNPADPEAGGSSKPGADDPFTAEALDWFVRLQGQSGDPATSRAFRAWLDGDPRRAAAFEKVTEVWGAAEFLQATKNVADATGVAAPRQKTRRRSLAKKAAAVASAVIVAFALAKGPDLSLRLQADYVTAAGEQRLVTLPDGSRLTLNTGSAVALDFANGQRDVRVLKGEAYFDVRSGLPIPFRVAGHFGDVEVKGTAFSVKLDDSEDNVVLSRGVVEVANRAHPREHASLSPGETVSVTATSISPVRKIDAERSLAWLEGRIRFDDRPFGEVLNDLRRYYPGRIFVATSAIEQVSVSGNYKLDQPLLVIQSLAEAAGATVTVLPGGIVVLR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-02-21" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNS6</accession>
    <name>HTPG_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chaperone protein HtpG</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Heat shock protein HtpG</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">High temperature protein G</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">htpG</name>
        <name type="ordered locus">Nwi_2812</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Molecular chaperone. Has ATPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 90 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316010.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SNS6"/>
    <dbReference type="SMR" id="Q3SNS6"/>
    <dbReference type="STRING" id="323098.Nwi_2812"/>
    <dbReference type="EnsemblBacteria" id="ABA06065">
        <property type="protein sequence ID" value="ABA06065"/>
        <property type="gene ID" value="Nwi_2812"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2812"/>
    <dbReference type="eggNOG" id="COG0326">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006684_3_0_5"/>
    <dbReference type="OMA" id="MRRMKEM"/>
    <dbReference type="OrthoDB" id="246194at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00505">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019805">
        <property type="entry name" value="Heat_shock_protein_90_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037196">
        <property type="entry name" value="HSP90_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001404">
        <property type="entry name" value="Hsp90_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020575">
        <property type="entry name" value="Hsp90_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11528">
        <property type="entry name" value="PTHR11528"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00183">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002583">
        <property type="entry name" value="Hsp90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00775">
        <property type="entry name" value="HEATSHOCK90"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110942">
        <property type="entry name" value="SSF110942"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00298">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0346">Stress response</keyword>
    <feature type="chain" id="PRO_0000224217" description="Chaperone protein HtpG">
        <location>
            <begin position="1"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="A; substrate-binding" evidence="1">
        <location>
            <begin position="1"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="B" evidence="1">
        <location>
            <begin position="339"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="C" evidence="1">
        <location>
            <begin position="553"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00505"/>
        </source>
    </evidence>
    <sequence length="637" mass="69663" checksum="807EC8DACAF3F842" modified="2005-10-11" version="1">MTSTIDKNGAAESRVFEADVAKLLQMMVHSVYSDKDVFLRELISNAADACERLRYEAISDPALLTDETRPRISITIDAERRQLAVEDNGIGMGRDELVDALGTIARSGTKAFIEQAEAAESGDGVALIGQFGVGFYSAFMVADQVDVVSRRAGAREAWRWSSDGKGTFTVTPVDESEAPARGTRVTLHLTEDATGYTDRLKIEQMIKEQSGHVPVPIALIEKPGAEPAEIADGAALWTRPRGEISASEYADFYRSVAGQFDEPALTVHFRAEGRQEFTALLFVPQTRPFDLFEPDKKRQLKLYVRRVFITEDADLLPRYLRFVRGVVDSADLPLNISREMIQESPILAAIKKSITGRILSELEKLADKDAQAYGKIWEAFGPMFKEGIYDAADRRDTVLGLSRFRTTAGSLRSLKDYVGALKENQTSIYYLAGQDAARLEASPHLEGFRARGVEVLLLSDPVDSFWVTSGPSFEGKPFKSVTQGAADLAAIPRLDAGTEPSPDVSEGVTEFLAFLKTTLSDLVSDVRSSDRLTDSPVCLVAAESGPDRQLEKILLGVGQLAGASKPVLEVNPNHPLVASLAALGQDDREFKEDAARMLLDDARVLDGDRPSDALEFSRRLIRLVERGLRRSSAGGGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNT1</accession>
    <name>Q3SNT1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2807</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2807"/>
    <dbReference type="EnsemblBacteria" id="ABA06060">
        <property type="protein sequence ID" value="ABA06060"/>
        <property type="gene ID" value="Nwi_2807"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2807"/>
    <dbReference type="eggNOG" id="COG5342">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100562_0_1_5"/>
    <dbReference type="OMA" id="WIKNAAE"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038696">
        <property type="entry name" value="IalB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010642">
        <property type="entry name" value="Invasion_prot_B"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06776">
        <property type="entry name" value="IalB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229117" evidence="1">
        <location>
            <begin position="26"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="182" mass="19191" checksum="E96DC41668194204" modified="2005-10-11" version="1">MMSMRQLLTVLVGSLLAGGLGLAPAFSAPTAGSSEPTLLGQFGAWGAYTASPNGKKVCFALSKPTSSRTNPPNRPRDTAYAFVSTRPAEKVTNEVSVTMGYPLKPGSEATVEVSGTSYAMYTQGDGIWIKNAADEDRLIDAMRKGSEATVKGMSSRGTQTTDVFSLKGLSQALDRVAQDCKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-09-02" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNT2</accession>
    <name>RLMN_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dual-specificity RNA methyltransferase RlmN</fullName>
            <ecNumber evidence="1">2.1.1.192</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">23S rRNA (adenine(2503)-C(2))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">23S rRNA m2A2503 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Ribosomal RNA large subunit methyltransferase N</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA (adenine(37)-C(2))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA m2A37 methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rlmN</name>
        <name type="ordered locus">Nwi_2806</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs. m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosine(2503) in 23S rRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2-methyladenosine(2503) in 23S rRNA + 5'-deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42916"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10152"/>
            <dbReference type="Rhea" id="RHEA-COMP:10282"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74497"/>
            <dbReference type="EC" id="2.1.1.192"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:43332"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10485"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74497"/>
            <dbReference type="EC" id="2.1.1.192"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the radical SAM superfamily. RlmN family.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="ABA06059" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.1.1.192" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06059.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_041345675.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SNT2"/>
    <dbReference type="SMR" id="Q3SNT2"/>
    <dbReference type="STRING" id="323098.Nwi_2806"/>
    <dbReference type="EnsemblBacteria" id="ABA06059">
        <property type="protein sequence ID" value="ABA06059"/>
        <property type="gene ID" value="Nwi_2806"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2806"/>
    <dbReference type="eggNOG" id="COG0820">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029101_0_0_5"/>
    <dbReference type="OrthoDB" id="1111428at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070040">
        <property type="term" value="F:rRNA (adenine-C2-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002935">
        <property type="term" value="F:tRNA (adenine-C2-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01849">
        <property type="entry name" value="RNA_methyltr_RlmN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040072">
        <property type="entry name" value="Methyltransferase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027492">
        <property type="entry name" value="RNA_MTrfase_RlmN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004383">
        <property type="entry name" value="rRNA_lsu_MTrfase_RlmN/Cfr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30544">
        <property type="entry name" value="PTHR30544"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006004">
        <property type="entry name" value="CHP00048"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00275">
        <property type="entry name" value="adenosine_C2_methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00048">
        <property type="entry name" value="rRNA_mod_RlmN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000350283" description="Dual-specificity RNA methyltransferase RlmN">
        <location>
            <begin position="1"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="126"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <feature type="active site" description="S-methylcysteine intermediate" evidence="1">
        <location>
            <position position="372"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="198"/>
            <end position="199"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="252"/>
            <end position="254"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="(transient)" evidence="1">
        <location>
            <begin position="133"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="397" mass="44466" checksum="F912AD1F68C8EEF8" modified="2008-09-02" version="2">MAEGGVPLEKTAIGTYVPPAKPSLIGLSRAEMAERLAAIGIPREQRRMRVQQLWHWMYVRGARTFAEMTSVSKDMRAELEKHVTLDRPEVVAEQISSDGTRKWLLRLPSGDDLEKPHEVECVYIPETDRGTLCVSSQVGCTLNCSFCHTGTQRLVRNLTAGEIVGQIMVARDRLNDWADRETPHGNRLVTNIVMMGMGEPLYNFDAVRDGLLIVADNEGIGISKRRITLSTSGVVPNIVRAGEEIGVMLAISLHAVRDELRDELVPLNRKYPIAELMQACRDYPAASNAKRITFEYVMLKGVNDSLDDARRLVKLLNGIHAKINLIPFNPWPGTRYECSDWDQIEKFSEYVFNAGYSSPVRTPRGRDILAACGQLKSETEKLSARERQALRAMAMTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNV0</accession>
    <name>Q3SNV0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2788</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2788"/>
    <dbReference type="EnsemblBacteria" id="ABA06041">
        <property type="protein sequence ID" value="ABA06041"/>
        <property type="gene ID" value="Nwi_2788"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2788"/>
    <dbReference type="HOGENOM" id="CLU_180754_0_0_5"/>
    <dbReference type="OMA" id="HWGHRHH"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229066" evidence="1">
        <location>
            <begin position="28"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="94" mass="11289" checksum="62B6DA145FDA4F94" modified="2005-10-11" version="1">MLLKKILGFAALGALLFFAAPAQQAQAATPVSPGIVAGQSGAGKLTTNVQWGHHHHRHWRHQRHHRRHWRNHRHHRHNWNRHHHRHHHHHRHWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="126" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNX6</accession>
    <name>Q3SNX6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uroporphyrinogen-III C-methyltransferase</fullName>
            <ecNumber evidence="16">2.1.1.107</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">Nwi_2762</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NAD(+) + precorrin-2 = 2 H(+) + NADH + sirohydrochlorin</text>
            <dbReference type="Rhea" id="RHEA:15613"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58351"/>
            <dbReference type="ChEBI" id="CHEBI:58827"/>
            <dbReference type="EC" id="1.3.1.76"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="13">Porphyrin-containing compound metabolism; siroheme biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Porphyrin-containing compound metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.107" evidence="16"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315960.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2762"/>
    <dbReference type="EnsemblBacteria" id="ABA06015">
        <property type="protein sequence ID" value="ABA06015"/>
        <property type="gene ID" value="Nwi_2762"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2762"/>
    <dbReference type="eggNOG" id="COG0007">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1648">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011276_2_1_5"/>
    <dbReference type="OMA" id="KMTVVVY"/>
    <dbReference type="OrthoDB" id="1185397at2"/>
    <dbReference type="UniPathway" id="UPA00262">
        <property type="reaction ID" value="UER00211"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043115">
        <property type="term" value="F:precorrin-2 dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051266">
        <property type="term" value="F:sirohydrochlorin ferrochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004851">
        <property type="term" value="F:uroporphyrin-III C-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd11642">
        <property type="entry name" value="SUMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006366">
        <property type="entry name" value="CobA/CysG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037115">
        <property type="entry name" value="Sirohaem_synt_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012409">
        <property type="entry name" value="Sirohaem_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019478">
        <property type="entry name" value="Sirohaem_synthase_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006367">
        <property type="entry name" value="Sirohaem_synthase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003043">
        <property type="entry name" value="Uropor_MeTrfase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10414">
        <property type="entry name" value="CysG_dimeriser"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036426">
        <property type="entry name" value="Sirohaem_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01469">
        <property type="entry name" value="cobA_cysG_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01470">
        <property type="entry name" value="cysG_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00839">
        <property type="entry name" value="SUMT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 16" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CysG_dimeriser" evidence="15">
        <location>
            <begin position="151"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="TP_methylase" evidence="14">
        <location>
            <begin position="225"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF10414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ABA06015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="476" mass="50129" checksum="B31FEE8296DBC9CE" modified="2005-10-11" version="1">MKFLPIFLDTSRGPILLVGAGDPVRARLRLLLAAEARVRWHATDGDFAVSGLDAAAASRIEREESDPRNADLGGVVAVFCAGAGAIGESVAARALTAGVPVNVMDDPARSSFIVPAIVDRGDVVAAIGTGGASPVVARRVRERIEAVLPARIGDLAAMIGRWRKTIHDHIPDLPLRRRFWERVIDGPIGEAVLAGRANEAEGSLKAIADPSAYAGASVPGDIEGRVTLVGAGPGDPDLLTVKALRALQDADIVFYDELITPEILDRARRDAVRVPVGRRVGKPGIGQDAINRLLIDAARSGRRAVRLKGGDPFIFGRGGEEVEALREAGVRCSVIPGITAGLGAAAESGVPLTYRHEALRVTFLTAHKAHDASNVDWSTLTDRKMTVVVYMGITAAPAIRSGLLAAGRSPETPVGVFARATRPDAKTVVGILDHLPALVHDVEGGPAILVIGDVVSHSARWRNTNSFLQTLQMAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP03</accession>
    <name>Q3SP03_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Glyceraldehyde-3-phosphate dehydrogenase</fullName>
            <ecNumber evidence="9">1.2.1.12</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_2735</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.12" evidence="9"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315933.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2735"/>
    <dbReference type="EnsemblBacteria" id="ABA05988">
        <property type="protein sequence ID" value="ABA05988"/>
        <property type="gene ID" value="Nwi_2735"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2735"/>
    <dbReference type="eggNOG" id="COG0057">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030140_0_2_5"/>
    <dbReference type="OMA" id="NAKVLAW"/>
    <dbReference type="OrthoDB" id="944149at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004365">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006424">
        <property type="entry name" value="Glyceraldehyde-3-P_DH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43148">
        <property type="entry name" value="PTHR43148"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00044">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000149">
        <property type="entry name" value="GAP_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00078">
        <property type="entry name" value="G3PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01534">
        <property type="entry name" value="GAPDH-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gp_dh_N" evidence="8">
        <location>
            <begin position="3"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Glyceraldehyde 3-phosphate binding" evidence="4">
        <location>
            <begin position="152"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Glyceraldehyde 3-phosphate binding" evidence="4">
        <location>
            <begin position="211"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="12"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="site" description="Activates thiol group during catalysis" evidence="6">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="335" mass="36181" checksum="AE7EB6D848E869A8" modified="2005-10-11" version="1">MAVRVAINGFGRIGRNVLRAVYESGRDEIEIVAINDLGPVETNAHLLRFDSVHGRFPFEVKVEGDSIVIGKNKIKVTAERDPSKLPWKELGIDIAMECTGLFTAKEKASAHLTAGAKRVLVSAPAEGADLTVVYGVNHDKLTKDHLVVSNGSCTTNCLAPVAKVLNDLIGIETGFMTTIHAYTGDQPTLDTMHKDLYRGRAAGLSMVPTSTGAARAIGLVLPELKGKLDGVAIRVPTPNVSVVDLKVIAKRETSTNEINEAMKRASSQELKGILGTTDQPNVSSDFNHDSHSSTLHIDQTKVQNGTLVRVLSWYDNEWGFSNRMTDTAVAMAKLI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP12</accession>
    <name>RUVC_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Crossover junction endodeoxyribonuclease RuvC</fullName>
            <ecNumber evidence="1">3.1.21.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Holliday junction nuclease RuvC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Holliday junction resolvase RuvC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ruvC</name>
        <name type="ordered locus">Nwi_2726</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).</text>
            <dbReference type="EC" id="3.1.21.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RuvC family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.10" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315925.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SP12"/>
    <dbReference type="SMR" id="Q3SP12"/>
    <dbReference type="STRING" id="323098.Nwi_2726"/>
    <dbReference type="EnsemblBacteria" id="ABA05979">
        <property type="protein sequence ID" value="ABA05979"/>
        <property type="gene ID" value="Nwi_2726"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2726"/>
    <dbReference type="eggNOG" id="COG0817">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091257_1_0_5"/>
    <dbReference type="OMA" id="AICHIWR"/>
    <dbReference type="OrthoDB" id="1815080at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16962">
        <property type="entry name" value="RuvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00034">
        <property type="entry name" value="RuvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020563">
        <property type="entry name" value="X-over_junc_endoDNase_Mg_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002176">
        <property type="entry name" value="X-over_junc_endoDNase_RuvC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30194">
        <property type="entry name" value="PTHR30194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02075">
        <property type="entry name" value="RuvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00696">
        <property type="entry name" value="RSOLVASERUVC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00228">
        <property type="entry name" value="ruvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01321">
        <property type="entry name" value="RUVC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000225155" description="Crossover junction endodeoxyribonuclease RuvC">
        <location>
            <begin position="1"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00034"/>
        </source>
    </evidence>
    <sequence length="172" mass="17987" checksum="985F11932707214F" modified="2005-10-11" version="1">MISSPIRILGIDPGLRRTGWGVLEIDGNRLMFVGCGSVETREHMALASRLLAIHAGLGAVLDQFRPSEAAIEQTFVNKDGVATLKLGQARGVAMLAPALFGIAVAEYAPNQVKKTVVGAGHADKAQIQAMLKILLPKADPKSADAADALAIAITHAHHRKSAALAARMKAVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP15</accession>
    <name>Q3SP15_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">4-hydroxybenzoyl-CoA thioesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2723</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 4-hydroxybenzoyl-CoA thioesterase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315922.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2723"/>
    <dbReference type="EnsemblBacteria" id="ABA05976">
        <property type="protein sequence ID" value="ABA05976"/>
        <property type="gene ID" value="Nwi_2723"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2723"/>
    <dbReference type="eggNOG" id="COG0824">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101141_7_0_5"/>
    <dbReference type="OMA" id="YHASYLR"/>
    <dbReference type="OrthoDB" id="1786865at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016790">
        <property type="term" value="F:thiolester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014166">
        <property type="entry name" value="Tol-Pal_acyl-CoA_thioesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006684">
        <property type="entry name" value="YbgC/YbaW"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003230">
        <property type="entry name" value="YbgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02799">
        <property type="entry name" value="thio_ybgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00051">
        <property type="entry name" value="TIGR00051"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4HBT" evidence="2">
        <location>
            <begin position="34"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="153" mass="17354" checksum="7EE3A7ACB921C87A" modified="2005-10-11" version="1">MNAPPPTGHLDGAIRDGRHHMQIRVYYEDTDFSGLVYHASYLRFMERGRSNYLRLLGADQRALFAETESEAPGFAFVVRSMHLDFLKPAHMDDILDIVTRPLEVRGASITLHQEVKRGDTLLLDARVRVAFISDGRAKPIPRPLRIAMKADQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP18</accession>
    <name>Q3SP18_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tol-Pal system protein TolQ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tolQ</name>
        <name evidence="10" type="ordered locus">Nwi_2720</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the Tol-Pal system, which plays a role in outer membrane invagination during cell division and is important for maintaining outer membrane integrity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">The Tol-Pal system is composed of five core proteins: the inner membrane proteins TolA, TolQ and TolR, the periplasmic protein TolB and the outer membrane protein Pal. They form a network linking the inner and outer membranes and the peptidoglycan layer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell inner membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ExbB/TolQ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315919.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2720"/>
    <dbReference type="EnsemblBacteria" id="ABA05973">
        <property type="protein sequence ID" value="ABA05973"/>
        <property type="gene ID" value="Nwi_2720"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2720"/>
    <dbReference type="eggNOG" id="COG0811">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053325_2_2_5"/>
    <dbReference type="OMA" id="LASIWSW"/>
    <dbReference type="OrthoDB" id="1796180at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043213">
        <property type="term" value="P:bacteriocin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02202">
        <property type="entry name" value="TolQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002898">
        <property type="entry name" value="MotA_ExbB_proton_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014163">
        <property type="entry name" value="Tol-Pal_TolQ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01618">
        <property type="entry name" value="MotA_ExbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02796">
        <property type="entry name" value="tolQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="4 8" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="MotA_ExbB" evidence="9">
        <location>
            <begin position="117"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA05973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="237" mass="26015" checksum="F11A8714591E0D65" modified="2005-10-11" version="1">MNPADVAQSALPLASADVSLITLFFHAHWIVKAVMLGLLACSVWVWAIAIDKTFLYARTKRAMDRFEQAFWSGESIEDLYRALSAKPTQSMAACFVAAMREWKRSFESQARSIAGLQMRIEKVMNVSIAREVERLERRLLVLATVGSAGPFVGLFGTVWGIMSSFQSIAASKNTSLAVVAPGIAEALFATAIGLIAAIPATIFYNKFTAEVNRQAQRLEGFADEFSAILSRQIDERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP33</accession>
    <name>Q3SP33_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DNA-3-methyladenine glycosylase I</fullName>
            <ecNumber evidence="1">3.2.2.20</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2705</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.2.2.20" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315904.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2705"/>
    <dbReference type="EnsemblBacteria" id="ABA05958">
        <property type="protein sequence ID" value="ABA05958"/>
        <property type="gene ID" value="Nwi_2705"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2705"/>
    <dbReference type="eggNOG" id="COG2818">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083758_1_0_5"/>
    <dbReference type="OMA" id="YVAYHDT"/>
    <dbReference type="OrthoDB" id="1835478at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008725">
        <property type="term" value="F:DNA-3-methyladenine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005019">
        <property type="entry name" value="Adenine_glyco"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004597">
        <property type="entry name" value="Tag"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03352">
        <property type="entry name" value="Adenine_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00624">
        <property type="entry name" value="tag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="208" mass="23298" checksum="4B77B5A2FF7BA26E" modified="2005-10-11" version="1">MTPSARLHADGLTRCPWPGDDPLYLAYHDTEWGVPEYDDRALFEKLILDGFQAGLSWITILRKRDNFRRAFDGFLPEKIASYDEKKVHALMNDAGIVRNRAKIEGAVTSAKSYLAIMERGPGFSTLLWNFMDGQPKVNRFKTTANVPASTPLSIKISKELASRGFKFVGPTIVYAFMQATGMVNDHLVTCHCHETCGKQARTPRSATQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP77</accession>
    <name>Q3SP77_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Multicopper oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2661</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315864.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2661"/>
    <dbReference type="EnsemblBacteria" id="ABA05914">
        <property type="protein sequence ID" value="ABA05914"/>
        <property type="gene ID" value="Nwi_2661"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2661"/>
    <dbReference type="eggNOG" id="COG2132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009100_3_0_5"/>
    <dbReference type="OMA" id="RTRYERY"/>
    <dbReference type="OrthoDB" id="971126at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="PTHR11709"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="SSF49503"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="6">
        <location>
            <begin position="157"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="5">
        <location>
            <begin position="541"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="681"/>
            <end position="703"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="703" mass="75068" checksum="C96130A6ADFD6BF7" modified="2005-10-11" version="1">MSRGGSLARAGYLAGAFVGGVAIAGAIPAAAEINNPPVLQDERAPIAKPAFKKLAPAAPAPGSAEPVRGDRQLDLKIEYIDSQIYNPSKDGYDKVRLRGYTGKGVNPNDPFVSPTIEAIPGDTVRITLDNTLPAGPQPGDPGCMPGAQMPDIPHCFNGTNLHTHGLWVSPTGNSDNVLLSINPQTRFTYEYNIPSDHPSGTFWYHTHRHGSTALQVSSGMAGALIIRGDRPPSPTAHGDLDTLIPRSAIPERVLVMQQIQYACRGPAPKPGELGPIKQDADGRYICDPGDVGGIEGYDQFGPGTWPASGRYTSLNGLVFNPDNPLKAKQGEIERWRMIHAGVRDTISLQFFKVRDGKLKPGTKTLLKAGAAADFIKDTCGATPVPYMLVAADGLTMGAGQMTELATFQPGYRFDALVAFPDAGRYCVIDASAPKSGVVNGLDVGPRLLAVVDVAAGTPVKDIQSHITGKLLALAEANMPDDVKQTVIADLKNGTKLSSFTPHPDIADEEVRGNGTQELTFFIDTSGSDVKFEVGNTLNTADVKPYDPARIDRSLPLGKAQEWVLQSHFVSHPFHIHVNPFQIVSILDPNGKDVSAAGAIDDAGGTPPDPQYPGLKGVWKDTLWIKSLIPPSSAPGGIYTIKLRTRYQRYIGEYVLHCHILDHEDQGMMQNVAVVLPDQVDSPAQGDAARSGEHARVGGHKGHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPG5</accession>
    <name>Q3SPG5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Co-chaperonin GroES</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">10 kDa chaperonin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Chaperonin-10</fullName>
            <shortName evidence="3">Cpn10</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">groES</name>
        <name evidence="3" type="synonym">groS</name>
        <name evidence="5" type="ordered locus">Nwi_2573</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Together with the chaperonin GroEL, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding. GroES binds to the apical surface of the GroEL ring, thereby capping the opening of the GroEL channel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heptamer of 7 subunits arranged in a ring. Interacts with the chaperonin GroEL.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3 4">Belongs to the GroES chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315777.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2573"/>
    <dbReference type="EnsemblBacteria" id="ABA05826">
        <property type="protein sequence ID" value="ABA05826"/>
        <property type="gene ID" value="Nwi_2573"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2573"/>
    <dbReference type="eggNOG" id="COG0234">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132825_1_0_5"/>
    <dbReference type="OMA" id="LGIFEND"/>
    <dbReference type="OrthoDB" id="1965002at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00320">
        <property type="entry name" value="cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.33.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00580">
        <property type="entry name" value="CH10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020818">
        <property type="entry name" value="Chaperonin_GroES"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037124">
        <property type="entry name" value="Chaperonin_GroES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018369">
        <property type="entry name" value="Chaprnonin_Cpn10_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772">
        <property type="entry name" value="PTHR10772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00166">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00297">
        <property type="entry name" value="CHAPERONIN10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00883">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00681">
        <property type="entry name" value="CHAPERONINS_CPN10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="98" mass="10570" checksum="1305154615E640E9" modified="2005-10-11" version="1">MAKTKFRPLHDRVVVKRIDAEEKTKGGIIIPDSAKEKPSQGEVVAVGPGGRDETGKLTPVDIKVGDKVLFGKWSGTEIKLDGQELLIMKESDIMGVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPN0</accession>
    <name>Q3SPN0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MaoC-like dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2508</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315712.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2508"/>
    <dbReference type="EnsemblBacteria" id="ABA05761">
        <property type="protein sequence ID" value="ABA05761"/>
        <property type="gene ID" value="Nwi_2508"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2508"/>
    <dbReference type="eggNOG" id="COG2030">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094876_1_0_5"/>
    <dbReference type="OMA" id="MRMMYDG"/>
    <dbReference type="OrthoDB" id="1563802at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002539">
        <property type="entry name" value="MaoC-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01575">
        <property type="entry name" value="MaoC_dehydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MaoC-like" evidence="1">
        <location>
            <begin position="18"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="153" mass="17161" checksum="4D3DC4BDAA423E34" modified="2005-10-11" version="1">MILTFEDFPLGRFGTFGPRHVSRDEMIAFATEFDPQPMHLDEEAAKHTMLRGLAGSGWHLCSLMMRMMFDGYIGRTASLGSPGVDEVRWLSSLRPGDDLMLDVDVAATRVSRSRPEMGIVTFAMRMRNASDQVLLEAVSPIMIRRRAEQPAQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPR6</accession>
    <name>Q3SPR6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Collagen triple helix repeat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2472</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315680.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2472"/>
    <dbReference type="EnsemblBacteria" id="ABA05725">
        <property type="protein sequence ID" value="ABA05725"/>
        <property type="gene ID" value="Nwi_2472"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2472"/>
    <dbReference type="eggNOG" id="COG2931">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109822_0_0_5"/>
    <dbReference type="OMA" id="IEQVMPW"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0176">Collagen</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="183" mass="19089" checksum="6076294248E05C0C" modified="2005-10-11" version="1">MAGEKRGKQGPQGIPGIQGPPGRTGSQGKPGPRGAPGKPGAQGAAGKAGSQGKPGPDGKPGRDGKPGADGKQGPRGEQGPRGEQGSPGPRGEQGPQGPRGEQGPPGRLPSIEEVLPWIEKIIEAWGEHQRRQTIEAENRLLAEQQRIEAERSLVQTLQDNDVTNGGREKRKKQKRRLKGGGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPS1</accession>
    <name>Q3SPS1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Thiamine-phosphate synthase</fullName>
            <shortName evidence="8">TP synthase</shortName>
            <shortName evidence="8">TPS</shortName>
            <ecNumber evidence="8">2.5.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Thiamine-phosphate pyrophosphorylase</fullName>
            <shortName evidence="8">TMP pyrophosphorylase</shortName>
            <shortName evidence="8">TMP-PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">thiE</name>
        <name evidence="12" type="ordered locus">Nwi_2467</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8 9">
            <text>2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = CO2 + diphosphate + thiamine phosphate</text>
            <dbReference type="Rhea" id="RHEA:47848"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:62890"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 8 9">
            <text>2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = CO2 + diphosphate + thiamine phosphate</text>
            <dbReference type="Rhea" id="RHEA:47844"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:62899"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 8 9">
            <text>4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 4-methyl-5-(2-phosphooxyethyl)-thiazole + H(+) = diphosphate + thiamine phosphate</text>
            <dbReference type="Rhea" id="RHEA:22328"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:58296"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 8 10">Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine and 4-methyl-5-(2-phosphoethyl)-thiazole: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the thiamine-phosphate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.3" evidence="8"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315675.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2467"/>
    <dbReference type="EnsemblBacteria" id="ABA05720">
        <property type="protein sequence ID" value="ABA05720"/>
        <property type="gene ID" value="Nwi_2467"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2467"/>
    <dbReference type="eggNOG" id="COG0352">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018272_3_3_5"/>
    <dbReference type="OMA" id="ITAFQFR"/>
    <dbReference type="OrthoDB" id="461201at2"/>
    <dbReference type="UniPathway" id="UPA00060">
        <property type="reaction ID" value="UER00141"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004789">
        <property type="term" value="F:thiamine-phosphate diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00564">
        <property type="entry name" value="TMP_TenI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00097">
        <property type="entry name" value="TMP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036206">
        <property type="entry name" value="ThiamineP_synth_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022998">
        <property type="entry name" value="ThiamineP_synth_TenI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034291">
        <property type="entry name" value="TMP_synthase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02581">
        <property type="entry name" value="TMP-TENI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51391">
        <property type="entry name" value="SSF51391"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00693">
        <property type="entry name" value="thiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8 9" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="5 8 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TMP-TENI" evidence="11">
        <location>
            <begin position="24"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="HMP-PP binding" evidence="8">
        <location>
            <begin position="41"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="THZ-P binding" evidence="8">
        <location>
            <begin position="190"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine</name>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine</name>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine</name>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:62899"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA05720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="214" mass="23416" checksum="A473339969F1A853" modified="2005-10-11" version="1">MQYPDRSAYPDRNAYPDRFYPVVDTLDWVRRLTGLGVGTVQLRAKDLNDGEALQLVSDALEIVKDTPVRLVVNDYWRAAIVAGAKHLHLGQEDLATADVHEIRKAGLTLGLSTHDDAELETALAAEPDYIALGPIFPTTLKAMRFAPQGIPKITEWKQRVGNIPLVAIGGIKLEQAPEIYAAGADSIAVVSDITQNPDPDARVKAWLDQMIEPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ19</accession>
    <name>Q3SQ19_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Peptidase M24</fullName>
            <ecNumber evidence="5">3.4.11.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2368</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M24B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.9" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2368"/>
    <dbReference type="MEROPS" id="M24.009"/>
    <dbReference type="EnsemblBacteria" id="ABA05622">
        <property type="protein sequence ID" value="ABA05622"/>
        <property type="gene ID" value="Nwi_2368"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2368"/>
    <dbReference type="eggNOG" id="COG0006">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011781_2_1_5"/>
    <dbReference type="OMA" id="YRPGKWG"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110165">
        <property type="term" value="C:cellular anatomical entity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01085">
        <property type="entry name" value="APP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_NTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000587">
        <property type="entry name" value="Creatinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033740">
        <property type="entry name" value="Pept_M24B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032416">
        <property type="entry name" value="Peptidase_M24_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01321">
        <property type="entry name" value="Creatinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16188">
        <property type="entry name" value="Peptidase_M24_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="SSF53092"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="SSF55920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Creatinase_N" evidence="3">
        <location>
            <begin position="56"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M24" evidence="2">
        <location>
            <begin position="361"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M24_C" evidence="4">
        <location>
            <begin position="583"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="644" mass="70289" checksum="6DB7551659BD5FA7" modified="2005-10-11" version="1">MVQPADAEQRVTRRLVAWLHANGVHFARKRYMMPAMFEAYFQTFEDPEGGVALTARLAAFREELARRQLTGFVIPRADRQQNEYVPPSEERLAWLTGFTGSAGLAIVLATKAAVFVDGRYTLQAAQQVDVRAWSIASLVDPPPESWLAEHLAAGDRLGYDPWLHTSAAVERLAKACAKAGAELVPVDSNPIDSVWTDRPAPPLGPVTIHDAAFAGEAEADKLARIRAEMTKLGVDALVLSDSHAVAWTFNIRGADVSHTPLPLSYALAPKDGRPTIFIDRRKLSDSARSHLERNADVREPDELTDALTRTAQSGAAIALDKATAADALSRLITSAGGKPVGGNDPVALLKAVKNPTEIAGARAAHRRDAVALARFLAWIDREAPKGTLTEIDAVEALETFRRETGALKDVSFPTISGTGPNGAIVHYRVTRKSNRRIVPGDLLLIDSGAQYEDGTTDVTRTIAIGDPTDEMRDRFTRVLRGHIAIARAVFPDGATGAQLDTLARQFLWQAGLDFEHGTGHGVGSYLSVHEGPARISKLGTTPLRRGMILSNEPGYYKRDAFGIRIENLVLVTEAGIAGAEKPMNSFETLTLAPIDRRLIDHRISKKEVAWLNDYHARVRREVRPHLDEATKLWLDAATEPFSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ50</accession>
    <name>Q3SQ50_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2337</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CpxP/Spy family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315555.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2337"/>
    <dbReference type="EnsemblBacteria" id="ABA05591">
        <property type="protein sequence ID" value="ABA05591"/>
        <property type="gene ID" value="Nwi_2337"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2337"/>
    <dbReference type="eggNOG" id="ENOG50334FE">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105365_0_0_5"/>
    <dbReference type="OMA" id="DRANARM"/>
    <dbReference type="OrthoDB" id="2041204at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012899">
        <property type="entry name" value="LTXXQ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07813">
        <property type="entry name" value="LTXXQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0574">Periplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229119" evidence="6">
        <location>
            <begin position="24"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="167"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="88"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="192" mass="21512" checksum="BB1B874AEA068A5E" modified="2005-10-11" version="1">MKKKAILAGVAALAIAGSTAVYAHQHHPWSHQRVQLSPQDRTAIADARIAAVHAGLALTPDQEKLWPPVEQAIRDFAKLRIDRVNAKMQAKKEKKESKKDDAQKQQAAVDQVARLRAHADRMAETAAALKKIADAADPLYKTLDDGQKRRLMVLTRMNHSFGMGGWRHHHRFDRGGDRLDHSKDRDEDSGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ83</accession>
    <name>Q3SQ83_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2304</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05558">
        <property type="protein sequence ID" value="ABA05558"/>
        <property type="gene ID" value="Nwi_2304"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2304"/>
    <dbReference type="HOGENOM" id="CLU_1049015_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="265" mass="29763" checksum="E81751EADA6A7808" modified="2005-10-11" version="1">MRTFSSTPRAQIPLHSDSATLWQSTWLRKLPCPSCLRPRRSVSCLSRKGGASLPLTRQKSGSKLLFSYVNSLSNRTRTICCLWLMTETIASNQDIETGHGNRDMYRADRPRPRQGMGGLFFPNPMLRALSFLIEVRNEALDCCFDAFSSREPVIHPRVEPKDMLRSKTLWMRHDCTAHGSSNVLSPASPVATELEGGDHFFLNGRDRDTQAAGDLRIGHAVDLAEKKDALGATRQRLQRAQQRVEIHPARDGILRADYAPMHLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQG9</accession>
    <name>Q3SQG9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 10 11">Ribulose-phosphate 3-epimerase</fullName>
            <ecNumber evidence="7 10 11">5.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rpe</name>
        <name evidence="15" type="ordered locus">Nwi_2218</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10 11">
            <text>D-ribulose 5-phosphate = D-xylulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:13677"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="EC" id="5.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 13">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="10 13">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Carbohydrate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 10 11">Belongs to the ribulose-phosphate 3-epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.1" evidence="7 10 11"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315441.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2218"/>
    <dbReference type="EnsemblBacteria" id="ABA05472">
        <property type="protein sequence ID" value="ABA05472"/>
        <property type="gene ID" value="Nwi_2218"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2218"/>
    <dbReference type="eggNOG" id="COG0036">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054856_1_0_5"/>
    <dbReference type="OMA" id="ISVHYES"/>
    <dbReference type="OrthoDB" id="1697639at2"/>
    <dbReference type="UniPathway" id="UPA00076">
        <property type="reaction ID" value="UER00148"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004750">
        <property type="term" value="F:D-ribulose-phosphate 3-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019323">
        <property type="term" value="P:pentose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00429">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02227">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026019">
        <property type="entry name" value="Ribul_P_3_epim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000056">
        <property type="entry name" value="Ribul_P_3_epim-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11749">
        <property type="entry name" value="PTHR11749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00834">
        <property type="entry name" value="Ribul_P_3_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001461">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01163">
        <property type="entry name" value="rpe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01085">
        <property type="entry name" value="RIBUL_P_3_EPIMER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01086">
        <property type="entry name" value="RIBUL_P_3_EPIMER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="13" id="KW-0170">Cobalt</keyword>
    <keyword evidence="9 10 11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="8 10 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="10 14">
        <location>
            <begin position="149"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="180"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10 14">
        <location>
            <begin position="202"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10 12">
        <location>
            <position position="42"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10 12">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10 14">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 13 14">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR001461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABA05472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="233" mass="24542" checksum="E53299C6A1509E55" modified="2005-10-11" version="1">MSQTFAPRPLVIAPSILASDFSKLGDEVRAVDQGGAEWIHLDVMDGHFVPNISYGPDVIKAMRPHSKKIFDAHLMISPCDPYLEAFAKAGCDHITVHVEAGPHLHRSLQAIRNLGKKAGVTLNPGTPLSTVEYVLDMVDLVLVMSVNPGFGGQKFISSAVDKIRDLRAMIGGRPIDIEVDGGVGPDVAGDLAAAGANAFVAGSAVFKGGTMESYKANMDAIRNAAAAARGEIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQK4</accession>
    <name>Q3SQK4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2183</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315406.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05437">
        <property type="protein sequence ID" value="ABA05437"/>
        <property type="gene ID" value="Nwi_2183"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2183"/>
    <dbReference type="eggNOG" id="ENOG5030WQY">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151768_0_0_5"/>
    <dbReference type="OMA" id="AYRNDYI"/>
    <dbReference type="OrthoDB" id="1920095at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="129" mass="13810" checksum="6656ADB954234AAE" modified="2005-10-11" version="1">MPDRTANDRRLRLPAVVALLIMGGGLGGCANLGDSAVSGAFVDPAKYDLYDCPQLAQQRRYLATRTAELQGLIEKAHTGVAGVAVAEVAYRNDYITTRASAKLADETWRKNRCDEASAAQETPAGDARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQL1</accession>
    <name>Q3SQL1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 9">Serine acetyltransferase</fullName>
            <ecNumber evidence="4 9">2.3.1.30</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_2176</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>acetyl-CoA + L-serine = CoA + O-acetyl-L-serine</text>
            <dbReference type="Rhea" id="RHEA:24560"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58340"/>
            <dbReference type="EC" id="2.3.1.30"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.30" evidence="4 9"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315399.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2176"/>
    <dbReference type="EnsemblBacteria" id="ABA05430">
        <property type="protein sequence ID" value="ABA05430"/>
        <property type="gene ID" value="Nwi_2176"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2176"/>
    <dbReference type="eggNOG" id="COG1045">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051638_0_1_5"/>
    <dbReference type="OMA" id="HPKIRHG"/>
    <dbReference type="OrthoDB" id="1639008at2"/>
    <dbReference type="UniPathway" id="UPA00136">
        <property type="reaction ID" value="UER00199"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009001">
        <property type="term" value="F:serine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03354">
        <property type="entry name" value="LbH_SAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045304">
        <property type="entry name" value="LbH_SAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010493">
        <property type="entry name" value="Ser_AcTrfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042122">
        <property type="entry name" value="Ser_AcTrfase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005881">
        <property type="entry name" value="Ser_O-AcTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06426">
        <property type="entry name" value="SATase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000441">
        <property type="entry name" value="CysE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00971">
        <property type="entry name" value="SATase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01172">
        <property type="entry name" value="cysE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 9 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SATase_N" evidence="10">
        <location>
            <begin position="19"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA05430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="276" mass="30316" checksum="FDD021D98C551149" modified="2005-10-11" version="1">MAIQHVNPQNPKFAALDPIWDRVRSEAEDIVRREPELAAFIYSAVLHHDRLEAAVVHRVAERLDHSALSGDLIRQTFDEALRDDPDIGNAFRADLVAVYDRDPATARFIDPLLYFKGFQAIQTHRLAYWLFQKGRKDFAYYLQSRSSSVFQVDINPAARIGRGIFLDHATGFVVGETAVIEDDVSILHGVTLGGTGKENEDRHPKIRHGVMIGAGAKILGNIEVGHCARIAAGSVVVKPVPHNVTVAGVPARTIGPAGCSEPSRTMNQMLNATLET</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQN0</accession>
    <name>Q3SQN0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Enoyl-CoA hydratase/isomerase</fullName>
            <ecNumber evidence="3">4.2.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2157</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.2.1.17" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2157"/>
    <dbReference type="EnsemblBacteria" id="ABA05411">
        <property type="protein sequence ID" value="ABA05411"/>
        <property type="gene ID" value="Nwi_2157"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2157"/>
    <dbReference type="eggNOG" id="COG1024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_22_1_5"/>
    <dbReference type="OMA" id="GKPYIAF"/>
    <dbReference type="OrthoDB" id="1238555at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003860">
        <property type="term" value="F:3-hydroxyisobutyryl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045004">
        <property type="entry name" value="ECH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032259">
        <property type="entry name" value="HIBYL-CoA-H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43176">
        <property type="entry name" value="PTHR43176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16113">
        <property type="entry name" value="ECH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ECH_2" evidence="2">
        <location>
            <begin position="20"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="357" mass="38282" checksum="F44A05B3ABB5EA78" modified="2005-10-11" version="1">MMVATSNEPDLITRREGSAGIVRLNRPRAINAVTLDMFRGIHKALDEFEADPEVGLILIEGAGERGLCAGGDIRSLWESANAGGDLGRVMWREEYLLNARIAKFPKPYVAFMDGIVMGGGVGLSAHGAHRVVTERTRLSMPEVGLGFFPDVGGTWLLSRAPGEVGTYFGLTGQNMNGADAIYAGLADVSVPSRKLAALRETLVDLGARADSARVRAAIAGFAADESASSVTAIRPRIDAWFAHGRMADILTTLQRDGSELARSILKVLNEKSPRGLVVTLKLLRLARASATLEECLAREYRAALAVFASDDFREGVRAAIIDKDRNPKWSPAAIGDVTPEMIAPYFANAGADELVFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQU8</accession>
    <name>Q3SQU8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2086</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315321.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2086"/>
    <dbReference type="EnsemblBacteria" id="ABA05343">
        <property type="protein sequence ID" value="ABA05343"/>
        <property type="gene ID" value="Nwi_2086"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2086"/>
    <dbReference type="HOGENOM" id="CLU_2524187_0_0_5"/>
    <dbReference type="OrthoDB" id="1859640at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="84" mass="9467" checksum="98162B302136A26F" modified="2005-10-11" version="1">MTDEGAGSMYFSDDALKQLADGYAAFGGKLNTLLEKYILLDLRNPRAREFAQQGFPRRLKVMARCISNVFEAIPPERNRTALAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQX1</accession>
    <name>Q3SQX1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">RNA methyltransferase TrmH, group 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_2062</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315299.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2062"/>
    <dbReference type="EnsemblBacteria" id="ABA05320">
        <property type="protein sequence ID" value="ABA05320"/>
        <property type="gene ID" value="Nwi_2062"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2062"/>
    <dbReference type="eggNOG" id="COG0566">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021322_0_2_5"/>
    <dbReference type="OMA" id="IFRSAYC"/>
    <dbReference type="OrthoDB" id="1422015at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="L30e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004441">
        <property type="entry name" value="rRNA_MeTrfase_TrmH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013123">
        <property type="entry name" value="SpoU_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46429">
        <property type="entry name" value="PTHR46429"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08032">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00967">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="SSF55315"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SpoU_sub_bind" evidence="5">
        <location>
            <begin position="50"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="283" mass="30268" checksum="A797FB5AACF38A24" modified="2005-10-11" version="1">MSDRDREPRFRRASRPRSDIHTKEAGGRNHQPKRGSARKARETSPDGPAVLYGWHTVTAALANPRRRIRKLLVTENAARRLTHENIDTPITPEIVRPSAIDQRLGPDAVHQGLLAEADPLPSPDIETLTQEGIVLVLDQITDPHNVGAIMRSAAAFSVKAIVTTARHSPEATGVLAKSASGALEIVPLVLVQNLARALAGLNDRGFMTVGLDSAGSENLAAVPLREPLALVLGAEGKGLRQLTRDTCSAVARLDMPGHIKSLNVSNAAVLALYIGASRLGLMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR26</accession>
    <name>Q3SR26_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Acyl-CoA dehydrogenase</fullName>
            <ecNumber evidence="9">1.3.8.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_2006</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.4" evidence="9"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315251.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2006"/>
    <dbReference type="EnsemblBacteria" id="ABA05265">
        <property type="protein sequence ID" value="ABA05265"/>
        <property type="gene ID" value="Nwi_2006"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2006"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016513_0_0_5"/>
    <dbReference type="OMA" id="GNVQCLD"/>
    <dbReference type="OrthoDB" id="433393at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008470">
        <property type="term" value="F:isovaleryl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041504">
        <property type="entry name" value="AidB_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18158">
        <property type="entry name" value="AidB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0274">FAD</keyword>
    <keyword evidence="3 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AidB_N" evidence="8">
        <location>
            <begin position="12"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="7">
        <location>
            <begin position="185"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="6">
        <location>
            <begin position="288"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="546" mass="59224" checksum="48427F7D743B83E6" modified="2005-10-11" version="1">MTQPFRTHEVLNQSPPFEDVDLFTLDRPLAEAVAANGGAAARDDMKAFGKHWGSSAMAARGRLANENPPKPRLFDARGNRRDEVEFHPSYHELMAHSAHAGLHNSTWTADGRPAAGAAEVVRAAKFYMAAQVETGHLCPITMTRASVATLAAQPDLLAETMPVIATRSYDPLFAPWRTKRGMTLGMGMTEKQGGTDVRANQTRAVRDGDAYRVTGHKWFMSAPMCDAFLVLAQAEGGLTCFFMPRFRPDGSVNAIHLQRLKDKLGNRSNASAEVEFIEAYARRVGEEGKGIRAILQMVQLTRQDCAIASAGLMRSGLAHALHHARHRSVFRKRLVDQPLMQAVLADMALQVEASVALVMRLCRAFDEAPSDQKAAVWMRLLTPAVKYWICKSAPGFLYEAMECLGGNGYVEEGILARHYREAPVNAIWEGSGNVICLDVLRSLARDGDAAVAVLDDLAADTRDLPGGAEAGSFLARTVRRPDGERAARQAVETLAQLAAAAALSRTAPDQAALFAATRLGCPHGSLFGTAEMSVAQSRDLLERALP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR47</accession>
    <name>Q3SR47_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Carboxysome shell protein CsoS2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1985</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the CsoS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315231.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1985"/>
    <dbReference type="EnsemblBacteria" id="ABA05244">
        <property type="protein sequence ID" value="ABA05244"/>
        <property type="gene ID" value="Nwi_1985"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1985"/>
    <dbReference type="eggNOG" id="ENOG502Z8T4">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016451_0_0_5"/>
    <dbReference type="OMA" id="DEPGTCK"/>
    <dbReference type="OrthoDB" id="183361at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043886">
        <property type="term" value="F:structural constituent of carboxysome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020990">
        <property type="entry name" value="CSOS2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12288">
        <property type="entry name" value="CsoS2_M"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="688"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="734"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="762"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="796"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="833"/>
            <end position="851"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="900" mass="93552" checksum="51DB67F4674E705A" modified="2005-10-11" version="1">MTNVAPGHATATLSGRELALQRRQAMARHGKAGVALAQADKRSASGRMQLNAVHVASATRSDASAASMATSAPAAIIQPAAPKSASRARRQALSTVGKAALKNAGQGVASRPAAHVRPRRAAHAEVPNSDCGCGCGGQCSCGGQCGCGQGAQRKEAAQIEAARIDTPAAARPAVSSPEASGRALAKMRRAALTQDGKKGLKRVAQATRIAASMPAQDWQSAMLKGATGRQVAMQHRMVRALAGRVDKASGAGTRPTGRIRARNVVEVPAKVEEGHTLSGQTVTGTQVERISKVTGNEPGSCRAITGTEYIGFEQFQTLCGVRPEPTAPKVAVSSTLREQRITGTALGRSTRTTGDEAGACRPVTGTEYLSAERFEQFCSARPDARPEKVALTRTDKGKTVTGALVDRPRRVTGVEHGADRAITGTRYSQGETDDAPPKVAVTHTAAGKPVTGTVVGRSERQTGDEAGSCRPITGTEYHTVEQFRDFCRTPPPGGGPRKVSVMSTRDEQVVSGTAVDRSAKVTGNEVGSCRSITGSQYYTTADFAGLCKTPSPRKVSEMQTLRGRTVTGSEVAPSPKLSGDESYGCEPVTGTDYIGTRQLAAVCPVEAAPAVSPVSKVVVDTTLRGQTITGIAAGRAEHVTGNEAGTCSPISGTPYIGQGQYTGFCQAPQREQQAARVRDSAVIPSTVVTGDRPGAGGSQVTGDERGACEPISGTPYLGLDNISTGQCAMSDRFRARDADQRPPAPRDFSIDIPARQAARERQTEVITGNGLSSSRITGPINKADGLITGTPEFRHGGQSRRPRDEEHSLPAARRLSGEGSQQGRRVSGDAWDGNSRVTGTEGSSSLARNPSMRGQPRGAGMSAMAFRGVERPEVPESVITGSAGNTRRGATVTVSGGARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR60</accession>
    <name>Q3SR60_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1972</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315218.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05231">
        <property type="protein sequence ID" value="ABA05231"/>
        <property type="gene ID" value="Nwi_1972"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1972"/>
    <dbReference type="HOGENOM" id="CLU_1813790_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="143" mass="15751" checksum="F038DCE50538155F" modified="2005-10-11" version="1">MSRGFGAIQRAVLDALSDKLIITLSTLDLAHIVNGGVRSRSMIASVHRALRTLEAAGKVVRLDGRWALASRQREARSYRYRQQPESPVQQRNERLKLISKILGMLGSSQDGEVLAAAKRAEAKRVELGLSWDDIILGDSCSII</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR94</accession>
    <name>Q3SR94_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CRISPR-associated exonuclease Cas4</fullName>
            <ecNumber evidence="6">3.1.12.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_1937</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="6">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="6">Mg(2+) or Mn(2+) required for ssDNA cleavage activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>iron-sulfur cluster</name>
            <dbReference type="ChEBI" id="CHEBI:30408"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CRISPR-associated exonuclease Cas4 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.12.1" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1937"/>
    <dbReference type="EnsemblBacteria" id="ABA05197">
        <property type="protein sequence ID" value="ABA05197"/>
        <property type="gene ID" value="Nwi_1937"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1937"/>
    <dbReference type="eggNOG" id="COG1468">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102055_1_1_5"/>
    <dbReference type="OMA" id="EWDNDEL"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013343">
        <property type="entry name" value="CRISPR-assoc_prot_Cas4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022765">
        <property type="entry name" value="Dna2/Cas4_DUF83"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01930">
        <property type="entry name" value="Cas_Cas4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00372">
        <property type="entry name" value="cas4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="6 8" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0408">Iron</keyword>
    <keyword evidence="3 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 6" id="KW-0464">Manganese</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas_Cas4" evidence="7">
        <location>
            <begin position="11"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA05197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="216" mass="23417" checksum="418607E47ABD9EDB" modified="2005-10-11" version="1">MPDGGEPIPLSALQHAVYCMRQAALIHLERVWAENRFTAEGQVLHAVADKGGGRKARGLRRVMALPVACVRLNIAGVVDMVEFHPAGNGTEVAYPVEYKGGKPKLHRADEVQLCAQALCLEEMTGTAVPEGALFYAETRRRVAVPFDHGLRNLTETTAAELAVVFSSGATPPPTHKRERCRACSLRELCRPEAVTKPVLAWRSRMVSCLLAEGPGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRA1</accession>
    <name>Q3SRA1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Lytic transglycosylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_1930</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transglycosylase Slt family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the virb1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315186.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1930"/>
    <dbReference type="CAZy" id="GH23">
        <property type="family name" value="Glycoside Hydrolase Family 23"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05190">
        <property type="protein sequence ID" value="ABA05190"/>
        <property type="gene ID" value="Nwi_1930"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1930"/>
    <dbReference type="eggNOG" id="COG0741">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015184_0_1_5"/>
    <dbReference type="OMA" id="EWWIERR"/>
    <dbReference type="OrthoDB" id="859969at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008933">
        <property type="term" value="F:lytic transglycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000270">
        <property type="term" value="P:peptidoglycan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008939">
        <property type="entry name" value="Lytic_TGlycosylase_superhlx_U"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000189">
        <property type="entry name" value="Transglyc_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008258">
        <property type="entry name" value="Transglycosylase_SLT_dom_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01464">
        <property type="entry name" value="SLT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48435">
        <property type="entry name" value="SSF48435"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00922">
        <property type="entry name" value="TRANSGLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229143" evidence="5">
        <location>
            <begin position="32"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="domain" description="SLT" evidence="6">
        <location>
            <begin position="576"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="30"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="735" mass="80517" checksum="76E008698BF43E03" modified="2005-10-11" version="1">MTLPARASKLRTAALGASLVAAALWAGSAQAEKTAKVPAPKSRPSGNAVPKPAPGHGKTAAPRKDKATAAPIIQPATRRHATLPAPSARMPVPKAAMAATSSTPRADLAALDTIIDLTRRQRSSEATQVQAGMTDPVARKLAEWLILRSNDNGAPTERYRAFIDANPSWPSQSFLRRRAEAALWDDHRDAAAVLAWFENERPQSAKGRFALASALLARGDRARAEQLVREAWRTDTMSAAVERKALEMFGALITPSDHKARMDYMLYGEDRDAGMRAAQRLGRSHEALAKARIAADRRSSSLRALLERVPSELHRDPGYMLAYIQLLRREEKFAEAARLMQSAPRDPNRLYNVDQWWIERRLLARKMIDVGQYRTAYLIARDAALPARDIYKTEQEFTAGWIALRFLNDPNTAARHFARIGVGSVNPTALARAGYWQGRAAEAAGRTQEARRAYAAAAEQSTSYYGQLARAKLGLPQIELRNVPGSRSRGIERLEIVRATQLLYALGKGDIAIPILADMGANGDPDAVLGLGELAARHGDARGMLLAGKAALNRGLPFDFYAYPVSGIPPFRSIGPDVERSIIYAIARQESAFNPSVVSPANAYGLMQVTPDAGRYVCKKYGASFDLRRLKTEPAYNAALGAAELGGLLDDYRGSYILTFAAYNAGRGSVRKWIARYGDPRDPGVDAVDWVELIPFSETRNYVQRIMENLQVYRARFGGGSKLQIEADLHRGAIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="139" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRD4</accession>
    <name>Q3SRD4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">Lon protease</fullName>
            <ecNumber evidence="7 8">3.4.21.53</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">ATP-dependent protease La</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">lon</name>
        <name evidence="16" type="ordered locus">Nwi_1897</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8 11">
            <text>Hydrolysis of proteins in presence of ATP.</text>
            <dbReference type="EC" id="3.4.21.53"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7 8">Homohexamer. Organized in a ring with a central cavity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="induction">
        <text evidence="7">By heat shock.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7 8 11 12">Belongs to the peptidase S16 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.53" evidence="7 8"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315153.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1897"/>
    <dbReference type="MEROPS" id="S16.001"/>
    <dbReference type="EnsemblBacteria" id="ABA05157">
        <property type="protein sequence ID" value="ABA05157"/>
        <property type="gene ID" value="Nwi_1897"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1897"/>
    <dbReference type="eggNOG" id="COG0466">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004109_4_3_5"/>
    <dbReference type="OMA" id="GAWQVVD"/>
    <dbReference type="OrthoDB" id="128102at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01973">
        <property type="entry name" value="lon_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027543">
        <property type="entry name" value="Lon_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004815">
        <property type="entry name" value="Lon_bac/euk-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008268">
        <property type="entry name" value="Peptidase_S16_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10046">
        <property type="entry name" value="PTHR10046"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001174">
        <property type="entry name" value="Lon_proteas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00763">
        <property type="entry name" value="lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01046">
        <property type="entry name" value="LON_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 7 8" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 8" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6 7" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="15">
        <location>
            <begin position="18"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Lon proteolytic" evidence="14">
        <location>
            <begin position="597"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="214"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="active site" evidence="7 9 11">
        <location>
            <position position="684"/>
        </location>
    </feature>
    <feature type="active site" evidence="7 9 11">
        <location>
            <position position="727"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 10">
        <location>
            <begin position="362"/>
            <end position="369"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR001174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001174-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001174-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ABA05157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="808" mass="89114" checksum="7B1CEA9CB6CA933F" modified="2005-10-11" version="1">MTASKPRPAIVHGEIHAYPVLPLRDIVVFPHMIVPLFVGREKSIRALEEVMKNDGLIMLATQKNASDDDPAPDSIYKIGTLASVLQLLKLPDGTVKVLVEGLERARVEAYSDRTDYYEATAVALADTDTNSVEAEALSRSVVSDFESYVKLNKKISPEVVGVVQAITDFAKLGDTVASHLAAKIADRQGILETLSVTARLEKVLGLMESEISVLQVEKRIRSRVKRQMEKTQREYYLNEQMKAIQKELGDDEGRDELADLEEKIAKTKLSKEAREKAQHELKKLRQMSPMSAEATVVRNYLDWLLSIPWAKKSKVKKDLNAAQEVLDADHYGLEKVKDRIVEYLAVQSRANKLTGPILCLVGPPGVGKTSLGKSIARATGREFVRVSLGGVRDEAEIRGHRRTYIGSMPGKIIQSMRKAKTSNPLFLLDEIDKMGADFRGDPSSALLEVLDPEQNSTFNDHYLEVDYDLSNVMFLTTANTLNIPGPLMDRMEIIRIAGYTENEKVEIARKHLIPNALSKHGLDSKEWSINDEALLLIIRRYTREAGVRNLERELSTLARKAVKELMLSKKKSVEVTEKVVEDFLGVPKYRFGEIETDDQVGVVTGLAWTDVGGELLTIESVMMPGKGRMTVTGNLRDVMKESISAAASYVRSRAITFGIEPPLFERRDIHVHVPEGATPKDGPSAGVAMATTIVSVLTGIPIRRDIAMTGEITLRGRVLPIGGLKEKLLAAARGGIKTVLIPDENAKDLTEISEAIKGGLEIIPVARMDEVIAKALVRQPKPIVWEEDTKLPVNPDAADEAAGGLTAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRS1</accession>
    <name>Q3SRS1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FecR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1759</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1759"/>
    <dbReference type="EnsemblBacteria" id="ABA05020">
        <property type="protein sequence ID" value="ABA05020"/>
        <property type="gene ID" value="Nwi_1759"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1759"/>
    <dbReference type="eggNOG" id="COG4254">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_481344_0_0_5"/>
    <dbReference type="OMA" id="SPVGEMG"/>
    <dbReference type="OrthoDB" id="501011at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006860">
        <property type="entry name" value="FecR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04773">
        <property type="entry name" value="FecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229087" description="FecR domain-containing protein" evidence="2">
        <location>
            <begin position="26"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="domain" description="FecR" evidence="3">
        <location>
            <begin position="56"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="264"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="277"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="602" mass="61521" checksum="EE2263BBB0E122E0" modified="2005-10-11" version="1">MTRAGAHWIAMTAAALALGGAAAQAQQVGTASAVNPAATANLKTITIGQSIAHKERIQTRSSGSVQLLFLDKTSMTIGPNSDLTIDEYVYDPNANTGKLAATLGKGALRFVGGQISHSGDAEIKTGTALIGIRGGVMMTDGKGGIYAGYGRMTVSSGGQTVTLGAGEFTQTQAGGPPSPPGLPPPGFVQQLIASFQSAAGQTGGARAGSASAKNVAAAEHKATGSPGGLVTGSGAPPSPNPPTIIQQINAITSAFTEAIQTSNQVTGAQQIAHDHPPGGDHPPPTNNIPPPTNNNPPGPLRGYVGGLVYTSSSEGEYYSSHGENGEHGVSYEGPTNIDPVTGTAEITLDTANKRVQARLDTYRYDQNNPFTQLTETMHELFQFGSKGSTGPADSVYVDKDNFYANTPENHEVNGKKVSGILVSGNGIATHEVVADAIPNLTPCQCDYTRWGFWMADESDTSTSAPTSYKTTVGTWVAGRPVGSIGDVPTTGAATYTGHVVANVNNAGFVAGGFSNTVNFGARTGAVSVTNLDGTNYSGTVNFNTDPRNFGGTLNGDTGSRQMAIDGSFFQGVNNPVGEMGGRVGISGADGYLGSGIFAATKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS08</accession>
    <name>Q3SS08_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1672</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04933">
        <property type="protein sequence ID" value="ABA04933"/>
        <property type="gene ID" value="Nwi_1672"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1672"/>
    <dbReference type="HOGENOM" id="CLU_2437842_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="90" mass="10231" checksum="720B6D696BEE6582" modified="2005-10-11" version="1">MTEEGFTADRDRRQSVSFRTSAMVILRAKPLHQQLKPERKLRFLRPHVGPQPFADRSRDHVAGLRINRGGAFGGSVVHFSPVSYERDSDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS24</accession>
    <name>Q3SS24_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Peptidase C14, caspase catalytic subunit p20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1656</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314916.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1656"/>
    <dbReference type="EnsemblBacteria" id="ABA04917">
        <property type="protein sequence ID" value="ABA04917"/>
        <property type="gene ID" value="Nwi_1656"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1656"/>
    <dbReference type="eggNOG" id="COG4249">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_542761_0_0_5"/>
    <dbReference type="OMA" id="FNGLLVY"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029030">
        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001309">
        <property type="entry name" value="Pept_C14_p20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="SSF52129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50208">
        <property type="entry name" value="CASPASE_P20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229185" evidence="1">
        <location>
            <begin position="28"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="CASPASE_P20" evidence="2">
        <location>
            <begin position="28"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="499" mass="54914" checksum="FA8CBA77DE1171A5" modified="2005-10-11" version="1">MRGISVGRTALAGIVCALAMLAGPALAAKRVALVIGNNDYRNVPKLQKAVNDARTMGDTLRQLGFSVMVAENQTRQDFSRTLLAFDRQIEAGDTAFFFYAGHGFEIAGQNYLLPTDVPAATEGQEELVRDASILADRIVERLQNRKARTSILVLDACRNNPFERKGTRAVAGGAGLAPMIELPEGVFSVFSAGPRQTALDRLSDNDSNPNSVFTRIFAKELLQPGENMVRVAQRTRRIVSEMADTVRHKQVPVYFDQMVDDVFLNGLAKAAQPKPAPTEPAPKIAALTPVAPLKPPSNEALNAPIANFSRHNGGWTVVFSIADPALGISWRMDENGEFKETGFLDTLDQRTRKRMPNPAIELPADAPAATIQMRYVDANGEMKGPFPIRFDPDAALIRDQRKILDMTATSWLSFRQFNGLLVYYTHLMSYRCAIREVKIGIDSSVPDRTLKMPPCDPRDPVAIPSNATPYLKLPPATKTVSVELTYRDGSVSEIKSFRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS32</accession>
    <name>Q3SS32_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminotransferase</fullName>
            <ecNumber evidence="1">2.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1648</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314908.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1648"/>
    <dbReference type="EnsemblBacteria" id="ABA04909">
        <property type="protein sequence ID" value="ABA04909"/>
        <property type="gene ID" value="Nwi_1648"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1648"/>
    <dbReference type="eggNOG" id="COG0436">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_4_5_5"/>
    <dbReference type="OMA" id="YPHMPTG"/>
    <dbReference type="OrthoDB" id="417859at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901605">
        <property type="term" value="P:alpha-amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="2">
        <location>
            <begin position="31"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="405" mass="44509" checksum="D3F6370B08F86CFF" modified="2005-10-11" version="1">MEEFYRIRRLPPYVFEQINRAKAAARNAGADIIDLGMGNPDLPTPPHVIEKLKETLGKPKTDRYSASRGITGLRRAQAGYYARRFGVKLNPDTQIVATLGSKEGFANVAQAITAPGDVILCPNPSYPIHAFGFLMAGGVIRSVPSSPTPEFFAACERAIIHSIPKPIGLIVCYPSNPTAQVACLDFYKDLVAFAKKHEIFILSDLAYSEVYFDDNPPPSVLQVPGALDVTVEFTSMSKTFSMAGWRIGFAVGNERIIAALARVKSYLDYGAFTPVQVAATAALNGPDTCIREMRETYRKRRDTLVESFGRAGWVIPPPQASMFAWAPLPERFRDLGSMQFSTLMVEKSGVVVSPGVGFGEHGEGYVRIAMVENEQRIRQAARGVRHFLESGIETLHNVVPLANRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS48</accession>
    <name>Q3SS48_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH_17 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1632</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1632"/>
    <dbReference type="EnsemblBacteria" id="ABA04893">
        <property type="protein sequence ID" value="ABA04893"/>
        <property type="gene ID" value="Nwi_1632"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1632"/>
    <dbReference type="eggNOG" id="COG3311">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2233678_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041657">
        <property type="entry name" value="HTH_17"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12728">
        <property type="entry name" value="HTH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_17" evidence="1">
        <location>
            <begin position="48"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="105" mass="12002" checksum="534B1FF8C701B037" modified="2005-10-11" version="1">MHPPERKIREWKPALPHEQVEACGFGGIDTGPGYCMSSHRRIKMNSVTIQEFCDSQRISRAYFYILAREGKAPRSYKLGKTTRISEDAIREWIAAREAESMEVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSA1</accession>
    <name>Q3SSA1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1579</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314846.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1579"/>
    <dbReference type="EnsemblBacteria" id="ABA04840">
        <property type="protein sequence ID" value="ABA04840"/>
        <property type="gene ID" value="Nwi_1579"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1579"/>
    <dbReference type="eggNOG" id="COG5482">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075295_0_0_5"/>
    <dbReference type="OMA" id="IMTAYRQ"/>
    <dbReference type="OrthoDB" id="1701649at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018679">
        <property type="entry name" value="DUF2161"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09929">
        <property type="entry name" value="DUF2161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="225" mass="24740" checksum="8295E583B063F8D5" modified="2005-10-11" version="1">METSLYLPVKRHLESLGFSVKGEVGGCDLVALNGDDPPVVVIGELKLSFNLELILQGVERAGACDEVWLAARLSMRGKGRESDARYRNLCRRLGFGMLGVTHQGNVEVLVAPSAASPRRNPKKRSRLVMEHQRRRGDPVAGGSTRMPIMTAYRQQALSCAAAMAAGPCRVRDLRGENPDAGKILLHNVYGWFDRVDRGIYCLTEAGKDALKRWPQQSCEAVPIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSA2</accession>
    <name>Q3SSA2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase</fullName>
            <ecNumber evidence="7">4.2.1.59</ecNumber>
            <ecNumber evidence="6">5.3.3.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabA</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Beta-hydroxydecanoyl thioester dehydrase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Trans-2-decenoyl-[acyl-carrier-protein] isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">Nwi_1578</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:13097"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="4.2.1.59"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the thioester dehydratase family. FabA subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.59" evidence="7"/>
    <dbReference type="EC" id="5.3.3.14" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314845.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1578"/>
    <dbReference type="EnsemblBacteria" id="ABA04839">
        <property type="protein sequence ID" value="ABA04839"/>
        <property type="gene ID" value="Nwi_1578"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1578"/>
    <dbReference type="eggNOG" id="COG0764">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097925_0_0_5"/>
    <dbReference type="OMA" id="GYWLGWS"/>
    <dbReference type="OrthoDB" id="1379832at2"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008659">
        <property type="term" value="F:(3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008693">
        <property type="term" value="F:3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047451">
        <property type="term" value="F:3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004317">
        <property type="term" value="F:3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034017">
        <property type="term" value="F:trans-2-decenoyl-acyl-carrier-protein isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01287">
        <property type="entry name" value="FabA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010083">
        <property type="entry name" value="FabA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013114">
        <property type="entry name" value="FabA_FabZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30272">
        <property type="entry name" value="PTHR30272"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30272:SF8">
        <property type="entry name" value="PTHR30272:SF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07977">
        <property type="entry name" value="FabA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01749">
        <property type="entry name" value="fabA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="15" id="KW-0456">Lyase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00017810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABA04839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="180" mass="20132" checksum="68B921BC6DC35427" modified="2005-10-11" version="1">MPNLRSSYDYEDLLACGRGEMFGPGEPKLPLPPMLMFDRITEITEGGGKYGRGLVRTELDIKPNLWFFQCHFQNDPVMPGCLGLDGLWQMVGFYLVWLGRDRNVRARALGLGDLKFTGQILPHARKISYTVDIKRVMRSKLVMAIADGTVSVDGDTIYHAKDLRLGLFKSLPGSPIEPTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSD0</accession>
    <name>Q3SSD0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314817.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1550"/>
    <dbReference type="EnsemblBacteria" id="ABA04811">
        <property type="protein sequence ID" value="ABA04811"/>
        <property type="gene ID" value="Nwi_1550"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1550"/>
    <dbReference type="eggNOG" id="ENOG5033S2P">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_692291_0_0_5"/>
    <dbReference type="OrthoDB" id="1779495at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="357"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="398" mass="44048" checksum="0A3B975254C17721" modified="2005-10-11" version="1">MIMPSPRYSIIPAGAVTDRSLEPRDLQVLCLLGRHTDDMGWCRRSQVKMSAELDCGRATIQRALERLVGAGWVQMKRRDLSDDDDTSRHPSASYAYRVLLDQEESGRGCPPASTPHPVPDSCGPAPEGVPIDEHPGAHPGRAPGAHTYVGTKNDPLERPHLERERERAREGKTEHESVARFLTTFEARWPTAALDDRQRTAYAAAALTAQQREAALGAVAGFLAEQKRRKRDRVPAGWKYLEQRRWELLSAAKPEAPASTVHHPPGSAAAAALATLHQIAMLETFFLKVHRLADGRVSWRGETTPRLLALANAPPRSQWIALNNQQAAAWDRYLADHVTVATRRRIMEGSMAPWPWPPRKDGTVYLETADEASPDESVASDDVIGNVAQHDQHGETRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSE8</accession>
    <name>Q3SSE8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase, family 2</fullName>
            <ecNumber evidence="2">2.4.1.83</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1532</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.1.83" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314799.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1532"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04793">
        <property type="protein sequence ID" value="ABA04793"/>
        <property type="gene ID" value="Nwi_1532"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1532"/>
    <dbReference type="eggNOG" id="COG0463">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033536_11_0_5"/>
    <dbReference type="OMA" id="FCIAGYR"/>
    <dbReference type="OrthoDB" id="1064289at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004582">
        <property type="term" value="F:dolichyl-phosphate beta-D-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="1">
        <location>
            <begin position="11"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="249" mass="27615" checksum="3635D2056DACE914" modified="2005-10-11" version="1">MSTRTTAPTVSIVVPVRNEADNVAPLIEEIAAALDGRWDYEIVYVDDGSTDATAERLTAIMAERGTVRQIRHEKSSGQSAAVRTGVRAARGPIVATLDGDGQNNPEFLPELIMAIEKGEGRIGLAAGQRVGRKDTGFKKFQSRVANKVRSRVLSDGTRDTGCGLKAFRREVFLSLPYFDGLHRFLPALVRREGYDIAYVDVTDRPRRSGVSNYGFFDRLWIGIIDLAGVWWLIRRKKSTPVATEVLRHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="128" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSG7</accession>
    <name>Q3SSG7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcription-repair-coupling factor</fullName>
            <shortName evidence="8">TRCF</shortName>
            <ecNumber evidence="8">3.6.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">mfd</name>
        <name evidence="11" type="ordered locus">Nwi_1513</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Couples transcription and DNA repair by recognizing RNA polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent release of RNAP and its truncated transcript from the DNA, and recruitment of nucleotide excision repair machinery to the damaged site.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">In the C-terminal section; belongs to the helicase family. RecG subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">In the N-terminal section; belongs to the UvrB family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="8"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314780.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1513"/>
    <dbReference type="EnsemblBacteria" id="ABA04774">
        <property type="protein sequence ID" value="ABA04774"/>
        <property type="gene ID" value="Nwi_1513"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1513"/>
    <dbReference type="eggNOG" id="COG1197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005122_0_3_5"/>
    <dbReference type="OMA" id="WAPPCRE"/>
    <dbReference type="OrthoDB" id="234717at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000716">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair, DNA damage recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.50">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036101">
        <property type="entry name" value="CarD-like/TRCF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003711">
        <property type="entry name" value="CarD-like/TRCF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004576">
        <property type="entry name" value="Mfd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037235">
        <property type="entry name" value="TRCF-like_C_D7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005118">
        <property type="entry name" value="TRCF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041471">
        <property type="entry name" value="UvrB_inter"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02559">
        <property type="entry name" value="CarD_CdnL_TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03461">
        <property type="entry name" value="TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17757">
        <property type="entry name" value="UvrB_inter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01058">
        <property type="entry name" value="CarD_TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00982">
        <property type="entry name" value="TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141259">
        <property type="entry name" value="SSF141259"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143517">
        <property type="entry name" value="SSF143517"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00580">
        <property type="entry name" value="mfd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="639"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="821"/>
            <end position="975"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA04774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1174" mass="130281" checksum="55A58D4C02D4D56A" modified="2005-10-11" version="1">MKAFSRSPAESLSPGRPLTLANVAEGAEGLVVSDLARAIKANATRRAAAVSLTVVCRDGPRMQQLARALEFFASDISVMQFPAWDCQPYDRVSPHGGVLAQRLTTLARLSRLTGSETPLIILTTVNAILQRVPAREIVASQALSVAPGNVVPMDSIAAWLEHNGYNRSSTVREPGEYAVRGGILDLFPSNLDHPVRFDFFGDSLESIRSFDPETQRTLFDVRSLDLVPISEFQFVTETIRRFRMGYVATFGAPERDDLLYEAVSEGRRYPGMEHWLPLFHERMDTLFDYLDGASIVIEPQGEDAASERFKQIADYHEARREPLEQAGGGVPYKPLPPDRLYLTPDEWAKRLNEAALVRLTPFAMPDESVNIIDAGARQGRNFAPERADTTLNVFESVGSHIQALQAARKKVVVTLWSEGSRDRMASMLKDHGLLNLTSINTWRTVHATPRNEVMLGVVGIESGFETESVAVISEQDILGDRLVRPPKASRKLENFISEVTSLAAGDLVVHVEHGIGRFVGLQTLEVGGAPHDCLELRYANETKLFLPVENIELLSRYGSDQANVELDRLGGGGWQARKAKLKSRIREIAGELIRIAAERHLREAPKLPVQSGLYDEFCARFPYEETEDQLGAIQASLEDLESGKPMDRLVCGDVGFGKTEVALRAAFAVALDGKQVAVVVPTTLLARQHAKTFTERFRGFPVNVAQASRLVSPKELAKVKKGIADGSVDIVVGTHALLGKSIKFRDLGLLVVDEEQHFGVSHKERLKQLRAHVHVLTLSATPIPRTLQLALTGVRDLSIIASPPVDRLAVRTFVAPHDPLMIREALLRERYRGGQAFYVVPRVSDLAEVKDFLDKHVPEMKVAVAHGQMPPTVIEDIISAFYDGKYDVLLSTTIVESGLDIPTANTLIVHRSDMFGLAQLYQLRGRVGRSKLRAYALFTLPSKHKITAQAERRLKVLQSLETLGAGFQLASHDLDIRGAGNLLGEEQSGHIKEVGFELYQSMLEEAIENLKTGVTEIADDRWSPQITIGMPVLIPEDYIADLAVRLSLYRRLADLDTDDEIDSFAAELRDRFGKLPDEVRYLFKVAAIKAYCRRANVEKVDAGPKGAVISFRDNKFAQPDRLVYFIRQHGQAAKVRPDMKVVFLQNWETPEERLMGTTEIMRQLANLAEDRKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSH5</accession>
    <name>Q3SSH5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Integral membrane protein TerC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1505</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314772.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1505"/>
    <dbReference type="EnsemblBacteria" id="ABA04766">
        <property type="protein sequence ID" value="ABA04766"/>
        <property type="gene ID" value="Nwi_1505"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1505"/>
    <dbReference type="eggNOG" id="COG0861">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064910_0_0_5"/>
    <dbReference type="OMA" id="RMPKHMQ"/>
    <dbReference type="OrthoDB" id="1917125at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005496">
        <property type="entry name" value="Integral_membrane_TerC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03741">
        <property type="entry name" value="TerC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="241" mass="26361" checksum="F779C6B6A48A80C8" modified="2005-10-11" version="1">MIELLTSPEAWAALVTLTALEIVLGIDNVIFLSVIVSRIPPKQAKRARQVGLALAVIFRIALLTVLVWLIGLTTPVLTVQDFAFSWRDIILIGGGLFLIAKATHEIHGEVEAREHEAGETPKPRAFLLVIVQIIIIDLVFSLDSIITAIGMAKDLEIMIAAVIIACFVMYLSSGPVSRFVSEHPTTKMLALAFLVLIGVALVADGFHFHIPRAYIYFAMAFSAAVEMFNVLAGRNRKKASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSI7</accession>
    <name>Q3SSI7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TOPRIM protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1493</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1493"/>
    <dbReference type="EnsemblBacteria" id="ABA04754">
        <property type="protein sequence ID" value="ABA04754"/>
        <property type="gene ID" value="Nwi_1493"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1493"/>
    <dbReference type="eggNOG" id="COG3598">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_626763_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01029">
        <property type="entry name" value="TOPRIM_primases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034154">
        <property type="entry name" value="TOPRIM_DnaG/twinkle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Toprim" evidence="1">
        <location>
            <begin position="156"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="437" mass="47892" checksum="DBE59F7F549AB498" modified="2005-10-11" version="1">MTLNFTTREIALALGGEISGRAVLAPGPNHSTKDRSLSIKVDPTAPDGFVVHSFAGDNPITCKDYVRDKLGLRWEPQKKNGVDPIRRMQERVSTPPAEYVYRLEDGTPYLRVKRTSEKQFYQSHWNGQAWVNGAPKGLKVPYRLPEMVQAARDGVMDIMIVEGEKDADNLAALGCIATTNSAGAEKFAPELAKWFKDRNVYVLPDNDEAGERHAKQVVETLQKVARSVRVVRLPGLPDKGDVSDWIEAGGTADELADLLRDAPAAEGPASRFKFETFSDLKTLPPAEYLMDGWIPERSVGLLYGRWGSGKSFLGFDWCLHLAFGLPDWHGAKLPGVPTDVLIIAREGHAGFVKRVNAFMQYRGISEDPQKLVFMRSSISFLDDAAFAALKDDIKACPSSDELRLFRGHGNGGSGSSGFEVRRPAGDAASVFGGWSGV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-07-25" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSM7</accession>
    <name>QUEC_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">7-cyano-7-deazaguanine synthase</fullName>
            <ecNumber evidence="1">6.3.4.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">7-cyano-7-carbaguanine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">PreQ(0) synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Queuosine biosynthesis protein QueC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">queC</name>
        <name type="ordered locus">Nwi_1453</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent conversion of 7-carboxy-7-deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7-carboxy-7-deazaguanine + ATP + NH4(+) = 7-cyano-7-deazaguanine + ADP + H(+) + H2O + phosphate</text>
            <dbReference type="Rhea" id="RHEA:27982"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:45075"/>
            <dbReference type="ChEBI" id="CHEBI:61036"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the QueC family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.20" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314721.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSM7"/>
    <dbReference type="SMR" id="Q3SSM7"/>
    <dbReference type="STRING" id="323098.Nwi_1453"/>
    <dbReference type="EnsemblBacteria" id="ABA04714">
        <property type="protein sequence ID" value="ABA04714"/>
        <property type="gene ID" value="Nwi_1453"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1453"/>
    <dbReference type="eggNOG" id="COG0603">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081854_0_0_5"/>
    <dbReference type="OMA" id="CESCMRR"/>
    <dbReference type="OrthoDB" id="1681374at2"/>
    <dbReference type="UniPathway" id="UPA00391"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008616">
        <property type="term" value="P:queuosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01995">
        <property type="entry name" value="ExsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01633">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018317">
        <property type="entry name" value="QueC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42914">
        <property type="entry name" value="PTHR42914"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06508">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006293">
        <property type="entry name" value="ExsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00364">
        <property type="entry name" value="TIGR00364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0671">Queuosine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000246866" description="7-cyano-7-deazaguanine synthase">
        <location>
            <begin position="1"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01633"/>
        </source>
    </evidence>
    <sequence length="238" mass="26088" checksum="B0DB8458434C3271" modified="2005-10-11" version="1">MIDEPASRTALVLFSGGQDSATCLAWALQRFARVETLGFDYGQRHAIELDCRDSLLAGMASLRPDWAKKLGETHTLAIPTLAEISDTALTRNVAIEVSESGLPNTFVPGRNLVFLTFAAALAYRRGVTDIVGGMCETDYSGYPDCRDETIRALRIALNLGMVRQFELHTPLMRLDKGATWSLAHELGGTGLVDLIREHSHTCYLGERGARHAWGYGCGECPACGLRAKGWREYLARIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSS5</accession>
    <name>Q3SSS5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314675.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1405"/>
    <dbReference type="EnsemblBacteria" id="ABA04666">
        <property type="protein sequence ID" value="ABA04666"/>
        <property type="gene ID" value="Nwi_1405"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1405"/>
    <dbReference type="eggNOG" id="COG1399">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088841_0_0_5"/>
    <dbReference type="OMA" id="GLDPYPR"/>
    <dbReference type="OrthoDB" id="1665714at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003772">
        <property type="entry name" value="YceD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02620">
        <property type="entry name" value="YceD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="196" mass="21186" checksum="8D06C25344907B18" modified="2005-10-11" version="1">MTAKDRPDTIPNPWISRAVVAQIPETGLHRDIEADEGVLMALAEIAGVRGIAYARASFDLMPGRDDRVHVRGRVSARVAQTCVVSLDPIENDVLEDIDLVFVPEGRIREFVVPINDEGGVGGEVPEAPEPIVNGVIDLGRLATDVLFLGIDPYPRKPNAVFAPPASATDPEDHPFAALKDLKVHPSPPEKKKPGRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SST9</accession>
    <name>Q3SST9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Periplasmic serine endoprotease DegP-like</fullName>
            <ecNumber evidence="5">3.4.21.107</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_1391</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>Acts on substrates that are at least partially unfolded. The cleavage site P1 residue is normally between a pair of hydrophobic residues, such as Val-|-Val.</text>
            <dbReference type="EC" id="3.4.21.107"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase S1C family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.107" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1391"/>
    <dbReference type="EnsemblBacteria" id="ABA04652">
        <property type="protein sequence ID" value="ABA04652"/>
        <property type="gene ID" value="Nwi_1391"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1391"/>
    <dbReference type="eggNOG" id="COG0265">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020120_1_1_5"/>
    <dbReference type="OMA" id="SYVGYAF"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011782">
        <property type="entry name" value="Pept_S1C_Do"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001940">
        <property type="entry name" value="Peptidase_S1C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00834">
        <property type="entry name" value="PROTEASES2C"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="SSF50494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02037">
        <property type="entry name" value="degP_htrA_DO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="PDZ" evidence="6">
        <location>
            <begin position="281"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="6">
        <location>
            <begin position="412"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="4">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="4">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="4">
        <location>
            <position position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR611782-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="496" mass="52981" checksum="782D5C89F097A66E" modified="2005-10-11" version="1">MSLFWRVFFTRAGIHFAPKHYISAILIPGIKFMTLLRLSAALLLSLTMLAPAHAQDRRVPASQAELRLSYAPIVQRVVPAVVNVYAAKMIQHRHPLLDDPIFRRFFGVPGGQPEQMQRSLGSGVMVDPSGLVVTNNHVIEGADQVKIALPDKREFEAEIVLKDTRTDLAVLRLKNVHEKFPTLDFANSDELQVGDVVMAIGNPFGVGQTVTQGIVSALARTQVGITDYQFFIQTDAAINPGNSGGALVDMTGHLVGINTAIFSRTGGSLGIGFAIPANMVRVVVASAKSGGKAVRRPWLGARLQAVTPEIAETIGLKAPAGALVASVAAGSPAARAGLKLSDLIVSIDGQPVDDPNAFDYRFATRPLGGTSQLEVQRNGKPLKLTVPLETAPDTSRDEVVITAPSPFQGAKVVNVSPGLADELHLDYGAEGVVIVALAESGMAANVGFRKGDIILAVNNKKIVKTTDLEQAVREARRLWRITLMRSGQQIQVMLGG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSY3</accession>
    <name>RL1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplA</name>
        <name type="ordered locus">Nwi_1347</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds directly to 23S rRNA. The L1 stalk is quite mobile in the ribosome, and is involved in E site tRNA release.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Protein L1 is also a translational repressor protein, it controls the translation of the L11 operon by binding to its mRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314626.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSY3"/>
    <dbReference type="SMR" id="Q3SSY3"/>
    <dbReference type="STRING" id="323098.Nwi_1347"/>
    <dbReference type="EnsemblBacteria" id="ABA04608">
        <property type="protein sequence ID" value="ABA04608"/>
        <property type="gene ID" value="Nwi_1347"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1347"/>
    <dbReference type="eggNOG" id="COG0081">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062853_0_0_5"/>
    <dbReference type="OMA" id="GWTDVDV"/>
    <dbReference type="OrthoDB" id="1671455at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01318_B">
        <property type="entry name" value="Ribosomal_L1_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005878">
        <property type="entry name" value="Ribosom_L1_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002143">
        <property type="entry name" value="Ribosomal_L1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_L1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_L1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_L1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023673">
        <property type="entry name" value="Ribosomal_L1_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002155">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="SSF56808"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01169">
        <property type="entry name" value="rplA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01199">
        <property type="entry name" value="RIBOSOMAL_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0678">Repressor</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0810">Translation regulation</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000230618" description="50S ribosomal protein L1">
        <location>
            <begin position="1"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01318"/>
        </source>
    </evidence>
    <sequence length="230" mass="23854" checksum="94153C6B6BF79646" modified="2005-10-11" version="1">MATGKRLKKSREGIDRNKLYPIADAIRMVKERATSKFDETIEIAMNLGVDPRHADQMVRGVVTLPNGTGRTLRVGVFARGAKAEEAKAAGADVVGAEDLVETVQGGTIAFDRCIATPDMMPLVGRLGKVLGPRGMMPNPKIGTVTMDVAGAVKGAKGGSVEFRVEKAGIVQAGIGKASFSAEKLVENVKALADAVAKAKPAGAKGTYVQRIAVSSTMGPGVKVEPGTVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST27</accession>
    <name>Q3ST27_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1303</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314584.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1303"/>
    <dbReference type="EnsemblBacteria" id="ABA04564">
        <property type="protein sequence ID" value="ABA04564"/>
        <property type="gene ID" value="Nwi_1303"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1303"/>
    <dbReference type="eggNOG" id="COG5467">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_146724_0_0_5"/>
    <dbReference type="OMA" id="QIRRTMD"/>
    <dbReference type="OrthoDB" id="2038949at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.790.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009945">
        <property type="entry name" value="ATPase_inh_sub_z"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038293">
        <property type="entry name" value="DUF1476_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07345">
        <property type="entry name" value="DUF1476"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031780">
        <property type="entry name" value="UCP031780"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="108" mass="11914" checksum="452988195E2315E2" modified="2005-10-11" version="1">MSTSFDKREEGFEKKFALDEEQKFKAEARRNKLLGLWVAEKLGITGDAANAYAKEVIAADFEQPGDDDVFQKVMGDLTAKGIALTEQQVRAQMNLFLAEAIAQVKAGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST63</accession>
    <name>Q3ST63_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Biopolymer transport protein ExbB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">Nwi_1267</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Involved in the TonB-dependent energy-dependent transport of various receptor-bound substrates. Protects ExbD from proteolytic degradation and functionally stabilizes TonB.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The accessory proteins ExbB and ExbD seem to form a complex with TonB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the exbB/tolQ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314556.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1267"/>
    <dbReference type="EnsemblBacteria" id="ABA04528">
        <property type="protein sequence ID" value="ABA04528"/>
        <property type="gene ID" value="Nwi_1267"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1267"/>
    <dbReference type="eggNOG" id="COG0811">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053325_0_2_5"/>
    <dbReference type="OMA" id="WTILIAK"/>
    <dbReference type="OrthoDB" id="1796180at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002898">
        <property type="entry name" value="MotA_ExbB_proton_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014164">
        <property type="entry name" value="TonB_ExbB_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01618">
        <property type="entry name" value="MotA_ExbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02797">
        <property type="entry name" value="exbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <keyword evidence="8 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 13" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229219" description="Biopolymer transport protein ExbB" evidence="16">
        <location>
            <begin position="27"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="104"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="211"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="254"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="MotA_ExbB" evidence="17">
        <location>
            <begin position="165"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="32"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="32"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="61"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ABA04528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="318" mass="32594" checksum="4A036AA0F0CA5BFB" modified="2005-10-11" version="1">MFRTSLRTISLAAALIMGSLVSSAIAQQNGSQQSVAHPTAPSAQTPAVSAPAPPVQAPPISNASEPATAATPSPAGTAGVNLLNPDTASPHELSPWSMFMSADIVVQLVMIGLAFASLVTWTICIAKLAELSLARRALRAALNKIGDARSLAEAQMALGAREGVLASLLASAMHEARLSAGISSDAGIKERAASSFAEIVRAESRRIRLGMGLLATIGATSPFVGLFGTVWGIMNSFIGISKSQTTNLAVVAPGIAEALLATALGLVAAIPAVIIYNYFARVTRGYTELVGRASGAAGRLLSRDLDRTHGSLTSRAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST99</accession>
    <name>Q3ST99_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acetyltransferase, GNAT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1230</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314521.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1230"/>
    <dbReference type="EnsemblBacteria" id="ABA04492">
        <property type="protein sequence ID" value="ABA04492"/>
        <property type="gene ID" value="Nwi_1230"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1230"/>
    <dbReference type="eggNOG" id="COG0454">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087351_0_0_5"/>
    <dbReference type="OMA" id="RIALFYC"/>
    <dbReference type="OrthoDB" id="1526822at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13673">
        <property type="entry name" value="Acetyltransf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="8"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="166" mass="17474" checksum="3127B28FF0C703FE" modified="2005-10-11" version="1">MGQSLPKPALRPMLPTDVPVLAAIFVASIEELTGDDYSEAQQIAWASAADDEDKFGARLSRQLTLVATLQRSPVGFASLKGTDHVDMLYVHPGAVEHGVATALCDAIEKLAGARGATSLTVEASDTAEGFFARRGYVAVQRNSVTIGDEWLANTTMKKTLAAGSPA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STA1</accession>
    <name>SYC_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cysteine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cysteinyl-tRNA synthetase</fullName>
            <shortName evidence="1">CysRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cysS</name>
        <name type="ordered locus">Nwi_1228</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys)</text>
            <dbReference type="Rhea" id="RHEA:17773"/>
            <dbReference type="Rhea" id="RHEA-COMP:9661"/>
            <dbReference type="Rhea" id="RHEA-COMP:9679"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78517"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.16" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314519.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3STA1"/>
    <dbReference type="SMR" id="Q3STA1"/>
    <dbReference type="STRING" id="323098.Nwi_1228"/>
    <dbReference type="EnsemblBacteria" id="ABA04490">
        <property type="protein sequence ID" value="ABA04490"/>
        <property type="gene ID" value="Nwi_1228"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1228"/>
    <dbReference type="eggNOG" id="COG0215">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013528_0_1_5"/>
    <dbReference type="OMA" id="MFALETE"/>
    <dbReference type="OrthoDB" id="952207at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004817">
        <property type="term" value="F:cysteine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006423">
        <property type="term" value="P:cysteinyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00672">
        <property type="entry name" value="CysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00041">
        <property type="entry name" value="Cys_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015803">
        <property type="entry name" value="Cys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024909">
        <property type="entry name" value="Cys-tRNA/MSH_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032678">
        <property type="entry name" value="tRNA-synt_1_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890">
        <property type="entry name" value="PTHR10890"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01406">
        <property type="entry name" value="tRNA-synt_1e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00983">
        <property type="entry name" value="TRNASYNTHCYS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00435">
        <property type="entry name" value="cysS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000240926" description="Cysteine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="282"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00041"/>
        </source>
    </evidence>
    <sequence length="477" mass="54151" checksum="18C3E7017E881E19" modified="2005-10-11" version="1">MDLRLYDTLTRRKRAFTPIDPSNVRMYVCGPTVYDFAHIGNARPVIVFDVLFRLLRHLYGDDHVTYARNITDVDDKINDRAARDFPDLPLNEAIRKVTEKTAAQFQADVAALGCLPPTHQPRATEFVLPRTDGKTDMVTFIKQLIARGHAYEAGGEVLFDVRSMPDYGALSGRRLDEQKAGARVAVDAHKRNPADFVLWKLSSENEPGWDSPWGRGRPGWHIECSAMSTAYLGDVFDIHGGGLDLIFPHHENEIAQSRCAHGTHAMANYWMHNGFLQVEGEKMSKSLGNFVTINELLTTEKFGGRKWPGEVLRLNMLRTHYRQPIDWTIKALEESETILRRVCGRIKQFEYARRGMPTKERSVQLNLPPEILEALVDDLNTPLMLSRIGELSYDDAFDHALLSIGIDVLSYARWLRSQEQYASSNVDELVVARLEARMRKDFKESDRIRDELAAMGVVLKDGKDADGKPVTTWEIAR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STB0</accession>
    <name>MOAA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GTP 3',8-cyclase</fullName>
            <ecNumber evidence="1">4.1.99.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Molybdenum cofactor biosynthesis protein A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">moaA</name>
        <name type="ordered locus">Nwi_1219</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AH2 + GTP + S-adenosyl-L-methionine = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + A + H(+) + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:49576"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.1.99.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer and homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the radical SAM superfamily. MoaA family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.22" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314510.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3STB0"/>
    <dbReference type="SMR" id="Q3STB0"/>
    <dbReference type="STRING" id="323098.Nwi_1219"/>
    <dbReference type="EnsemblBacteria" id="ABA04481">
        <property type="protein sequence ID" value="ABA04481"/>
        <property type="gene ID" value="Nwi_1219"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1219"/>
    <dbReference type="eggNOG" id="COG2896">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009273_0_1_5"/>
    <dbReference type="OMA" id="IEFMPIG"/>
    <dbReference type="OrthoDB" id="1199289at2"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019008">
        <property type="term" value="C:molybdopterin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061798">
        <property type="term" value="F:GTP 3',8'-cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01225_B">
        <property type="entry name" value="MoaA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013483">
        <property type="entry name" value="MoaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000385">
        <property type="entry name" value="MoaA_NifB_PqqE_Fe-S-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010505">
        <property type="entry name" value="Mob_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06463">
        <property type="entry name" value="Mob_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02666">
        <property type="entry name" value="moaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01305">
        <property type="entry name" value="MOAA_NIFB_PQQE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="chain" id="PRO_1000054208" description="GTP 3',8-cyclase">
        <location>
            <begin position="1"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="19"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="273"/>
            <end position="275"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <sequence length="343" mass="37975" checksum="888FA80AA3689C10" modified="2005-10-11" version="1">MTGCSAPAETSGSRKMVDPFGRNISYLRVSVTDRCDLRCFYCMSEDMTFLPKADLLTLEELDRLCSAFIAKGVRKLRLTGGEPLVRRNVMSLIRSLSRHLQSGALKELTLTTNGSQLVRFANELKDCGVKRINVSLDTLDPARFREITRWGDIQKVMAGIDAAQAAGLAVKINAVALKNLNEDEIPSLMEWAHSRDMALTLIEVMPMGDIGASRADQYLPLSMLRARLEGQYTLTDLDDSTGGPARYVRVSETGGKLGFITPMTHNFCESCNRVRITCTGTLHTCLGHEDASDLRRPLRASPTDELLYETIDRAIGLKPKGHDFIIDRHNRPSVSRHMSVTGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STB7</accession>
    <name>Q3STB7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_1212</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314503.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1212"/>
    <dbReference type="EnsemblBacteria" id="ABA04474">
        <property type="protein sequence ID" value="ABA04474"/>
        <property type="gene ID" value="Nwi_1212"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1212"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_75_5"/>
    <dbReference type="OMA" id="IWGDERA"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="158"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="5">
        <location>
            <begin position="270"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA04474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="536" mass="58445" checksum="85D066B455F8AEF2" modified="2005-10-11" version="1">MSTPADKPEVVELPVAPAIVPSARHRRATTQRVREARDRLTSTSGTRPAFDHELLRQYAQTRLSASFVVMLLLVATGVLFGFWMAPAAAAAWTFGMLCIHTVIIYNCKGYLKEPSSATSTRKWRARFILFDLLYGLCWTTILVHPAGSDPVTNTLMMFLMLLVVAVSSMLSASLPIAAFAATAPVTSAIAFKFAMGGSFNNYILAILTLTAEGFFALLAHRLHSTTLATLEARAEKDALIGELEQAKAISDEARHRAESANVAKSRFLAQMSHELRTPLNAILGFSEVMKSEIFGEHAVAVYKTYSADIHNSGVHLLNLINEILDLSRIEAGRYELNEEAVSLVHVVTECHHLLKLRASNRGITIHEIFEQGMPRIWGDERATRQIVLNLLSNAIKFTPQGGEIWLKVGWTASGGQYLSVKDNGSGIAEDEIPIVLASFGQGSNSIKSAEQGAGLGLPIAKSLIDMHGGTFTLRSKLRVGTEVIVTFPPERVMSALAPMAEEGPPLQPAQADDHTADERRRLRNKPIMNAGTGLRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STG1</accession>
    <name>Q3STG1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1168</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314459.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1168"/>
    <dbReference type="EnsemblBacteria" id="ABA04430">
        <property type="protein sequence ID" value="ABA04430"/>
        <property type="gene ID" value="Nwi_1168"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1168"/>
    <dbReference type="eggNOG" id="ENOG50338T7">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082056_0_0_5"/>
    <dbReference type="OMA" id="WRSPSEL"/>
    <dbReference type="OrthoDB" id="1977832at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="250" mass="27935" checksum="2AC5207C3A79BE87" modified="2005-10-11" version="1">MPSHHEAWLRHQQSRWLRPDAARWVRPDAARFLPPGADVAKAFPTLARKYNPNQPRVPAGNSDGGQWTDGGAGSLGLFQITPRNNAVDGVQLAGDWRPDDGPPPIPPKRPETTEGRMRFVRQAAKWLRVVGRLSPAADVFIGALDQAEDLKRMTNVIKTANDPPGTLGELQARAQLPSEAGYEDHHIVGQFAQNRQQFGDERIDSWGNTVRIPTLKHLDINGWYSTPSPDYGDLSPRDYLRGRAWDEQMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STI0</accession>
    <name>Q3STI0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF1214 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1149</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314441.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1149"/>
    <dbReference type="EnsemblBacteria" id="ABA04411">
        <property type="protein sequence ID" value="ABA04411"/>
        <property type="gene ID" value="Nwi_1149"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1149"/>
    <dbReference type="eggNOG" id="COG5402">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099815_1_0_5"/>
    <dbReference type="OMA" id="TQRDAPM"/>
    <dbReference type="OrthoDB" id="1239657at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010621">
        <property type="entry name" value="DUF1214"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037049">
        <property type="entry name" value="DUF1214_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012038">
        <property type="entry name" value="UCP009471"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06742">
        <property type="entry name" value="DUF1214"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009471">
        <property type="entry name" value="UCP009471"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1214" evidence="1">
        <location>
            <begin position="72"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="192" mass="20567" checksum="DE773BCA5619BB31" modified="2005-10-11" version="1">MRLIVITLLGLGLATALGLGATWITATRGTNLGTLTIGAWTARPRIGSAEIDPYSRAAIARSGELPIGAGDGIAFLAKADDAQRPLDGRCEVIIAGITPAARFWTLTLHDRKGYLVANSLQRYGFTSQEIVRNSDGSFLVHVASRARAGNWLPTGGIERYVLMLRLYDTPVGVGTRTRRDAPMPSITTEKCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STI4</accession>
    <name>Q3STI4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1145</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314437.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04407">
        <property type="protein sequence ID" value="ABA04407"/>
        <property type="gene ID" value="Nwi_1145"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1145"/>
    <dbReference type="eggNOG" id="COG5478">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072266_2_0_5"/>
    <dbReference type="OMA" id="DHTLREK"/>
    <dbReference type="OrthoDB" id="1852102at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007251">
        <property type="entry name" value="Iron_permease_Fet4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04120">
        <property type="entry name" value="Iron_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="162" mass="17819" checksum="0F0C509BC87F1A6B" modified="2005-10-11" version="1">MPTIWREVRSAVEQADNTNRSKSSKLFSEIANKASRATGRAPTFVAAVAVVLIWAVTGPLFGYSDTWQLVINTGTTIVTFLMVFLIQNSQNRDGAAIQAKLDELIRVSTARNSFVGIEHLTDDELEDIREKCERRAKAEKISEKAAHVTSKVAGQAVDRVAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STJ7</accession>
    <name>Q3STJ7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Flagellar basal body rod protein FlgB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_1132</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 7">Structural component of flagellum, the bacterial motility apparatus. Part of the rod structure of flagellar basal body.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">The basal body constitutes a major portion of the flagellar organelle and consists of a number of rings mounted on a central rod. In Gram-negative bacteria, at least four rings, L, P, S and M are present, whereas Gram-positive bacteria lack the L and P rings. The rod consists of about 26 subunits of FlgG in the distal portion, and FlgB, FlgC and FlgF build up the proximal portion of the rod with about 6 subunits each. Rod assembly occurs by export via the flagellum-specific pathway of its constituent proteins and by their incorporation into the rod structure in the probable order of FlgB, FlgC, FlgF and FlgG. Another protein, FliE, also assembles onto the stable rod structure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Bacterial flagellum basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the flagella basal body rod proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314425.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1132"/>
    <dbReference type="EnsemblBacteria" id="ABA04394">
        <property type="protein sequence ID" value="ABA04394"/>
        <property type="gene ID" value="Nwi_1132"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1132"/>
    <dbReference type="eggNOG" id="COG1815">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125463_2_1_5"/>
    <dbReference type="OMA" id="SANQMDY"/>
    <dbReference type="OrthoDB" id="1914857at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030694">
        <property type="term" value="C:bacterial-type flagellum basal body, rod"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001444">
        <property type="entry name" value="Flag_bb_rod_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019776">
        <property type="entry name" value="Flagellar_basal_body_rod_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006300">
        <property type="entry name" value="FlgB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30435:SF12">
        <property type="entry name" value="PTHR30435:SF12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00460">
        <property type="entry name" value="Flg_bb_rod"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002889">
        <property type="entry name" value="Rod_FlgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00588">
        <property type="entry name" value="FLAGELLA_BB_ROD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="10" id="KW-0282">Flagellum</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flg_bb_rod" evidence="9">
        <location>
            <begin position="14"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="71"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00026072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR002889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA04394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="135" mass="14294" checksum="99CD277BBA34A8B4" modified="2005-10-11" version="1">MPLNDISALAALRTRMQWHQERQRVLAENIANSDTPNFRPHDLVEPTFDAQGGNASSGVTLAMTRTTAGQSMAGSGASAHFGQNGNGGFQTRPAGNAVSLEDQMLQVSANQMDYAAAASLYSRSLRLLKMAAGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STX7</accession>
    <name>Q3STX7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">N5-carboxyaminoimidazole ribonucleotide mutase</fullName>
            <shortName evidence="3 4">N5-CAIR mutase</shortName>
            <ecNumber evidence="3 4">5.4.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">5-(carboxyamino)imidazole ribonucleotide mutase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">purE</name>
        <name evidence="7" type="ordered locus">Nwi_1002</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Catalyzes the conversion of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to 4-carboxy-5-aminoimidazole ribonucleotide (CAIR).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + H(+) = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate</text>
            <dbReference type="Rhea" id="RHEA:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58730"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="EC" id="5.4.99.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AIR carboxylase family. Class I subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.18" evidence="3 4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314302.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1002"/>
    <dbReference type="EnsemblBacteria" id="ABA04264">
        <property type="protein sequence ID" value="ABA04264"/>
        <property type="gene ID" value="Nwi_1002"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1002"/>
    <dbReference type="eggNOG" id="COG0041">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094982_2_2_5"/>
    <dbReference type="OMA" id="SNSIDGW"/>
    <dbReference type="OrthoDB" id="1701464at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00943"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034023">
        <property type="term" value="F:5-(carboxyamino)imidazole ribonucleotide mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01929">
        <property type="entry name" value="PurE_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033747">
        <property type="entry name" value="PurE_ClassI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000031">
        <property type="entry name" value="PurE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024694">
        <property type="entry name" value="PurE_prokaryotes"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00731">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001338">
        <property type="entry name" value="AIR_carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01001">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01162">
        <property type="entry name" value="purE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="1 3 4" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIRC" evidence="6">
        <location>
            <begin position="3"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR001338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001338-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="162" mass="16598" checksum="AC2F1A8B8F2025CB" modified="2005-10-11" version="1">MTAVVAIIMGSQSDWETMRHAAETLAALGVTSENRIVSAHRTPERLFAFARGAKAEGFKVIIAGAGGAAHLPGMTAALTELPVLGVPVESRALAGVDSLYSIVQMPAGVPVGTLAIGKAGAINAALLAASVLALNDPPLAGRLEAWRKRQTDAVAEHPEESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STY5</accession>
    <name>Q3STY5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">COX4_pro_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0994</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0994"/>
    <dbReference type="EnsemblBacteria" id="ABA04256">
        <property type="protein sequence ID" value="ABA04256"/>
        <property type="gene ID" value="Nwi_0994"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0994"/>
    <dbReference type="eggNOG" id="ENOG5033B1X">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_174805_1_0_5"/>
    <dbReference type="OMA" id="PAHENTY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036596">
        <property type="entry name" value="Cyt-C_aa3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012422">
        <property type="entry name" value="Cyt_c_oxidase_su4_bac-aa3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07835">
        <property type="entry name" value="COX4_pro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81469">
        <property type="entry name" value="SSF81469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="COX4_pro_2" evidence="2">
        <location>
            <begin position="4"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="50" mass="5441" checksum="FE029C2854D74C0A" modified="2005-10-11" version="1">MSDHSEIVYSTADGMDYPAHENTYNAFIKFGTIGSVAVIIVVSLMAIFLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STZ5</accession>
    <name>Q3STZ5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TPR repeat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0984</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0984"/>
    <dbReference type="EnsemblBacteria" id="ABA04246">
        <property type="protein sequence ID" value="ABA04246"/>
        <property type="gene ID" value="Nwi_0984"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0984"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079829_1_0_5"/>
    <dbReference type="OMA" id="WNRRATI"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229203" evidence="3">
        <location>
            <begin position="31"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="169"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="29"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="221" mass="24472" checksum="DD7CD7A836637E7B" modified="2005-10-11" version="1">MQPTTSSAGRLCLPMLFVLAGTLAPTLVSAQSSSRSPPQATPPEATQPSDPPRTLPRSPKERGRGLDFLFGALKAAPDEASARHVESRILTLWSQTSSDTISLLMQRVKTATDAKQSDVALKLLDAVLKLRPEYIEGWSRRATLHYVRNDYQRSLEDIEQVLIREPRHFGALAGLGVIMQELGDDKRALDAFRKALAVNPHLERVRELVKTLTEKVEGRDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU04</accession>
    <name>Q3SU04_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SirA-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0975</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfur carrier protein TusA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314277.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0975"/>
    <dbReference type="EnsemblBacteria" id="ABA04237">
        <property type="protein sequence ID" value="ABA04237"/>
        <property type="gene ID" value="Nwi_0975"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0975"/>
    <dbReference type="eggNOG" id="COG0425">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165255_1_0_5"/>
    <dbReference type="OMA" id="EVHCTDP"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001455">
        <property type="entry name" value="TusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036868">
        <property type="entry name" value="TusA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01206">
        <property type="entry name" value="TusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64307">
        <property type="entry name" value="SSF64307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01148">
        <property type="entry name" value="UPF0033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0033" evidence="2">
        <location>
            <begin position="10"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="101" mass="11259" checksum="63F19090FEA17DBE" modified="2005-10-11" version="1">MFVPMLISKLDLTGLKCPMPALKTRKALKSLTPGERLEVHCTDPLSVIDIPNLIRETGDSVEITERAERRIVFLIKKAGAPSSFEDRFGSLSLRSLCPYTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUG4</accession>
    <name>Q3SUG4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0812</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314125.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0812"/>
    <dbReference type="EnsemblBacteria" id="ABA04077">
        <property type="protein sequence ID" value="ABA04077"/>
        <property type="gene ID" value="Nwi_0812"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0812"/>
    <dbReference type="HOGENOM" id="CLU_2554849_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="82" mass="8796" checksum="286FE1B54C94D352" modified="2005-10-11" version="1">MKRRKVHPADAANAAVLANAVRYDVALFLGTGRYARASAPTLEDARIEAMRLVADNPTPFGKRLPLIYGVTAEGRSALVTSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUH8</accession>
    <name>Q3SUH8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0798</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0798"/>
    <dbReference type="EnsemblBacteria" id="ABA04063">
        <property type="protein sequence ID" value="ABA04063"/>
        <property type="gene ID" value="Nwi_0798"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0798"/>
    <dbReference type="eggNOG" id="ENOG5032FA9">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_191534_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="48" mass="5043" checksum="DD2BD83C21C95C68" modified="2005-10-11" version="1">MGGVYALDFGAILLLAHSMGALNPLVVDVLSEIEAVVVNAYRRNADPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUM4</accession>
    <name>Q3SUM4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Corrinoid adenosyltransferase</fullName>
            <ecNumber evidence="4">2.5.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Cob(II)alamin adenosyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Cob(II)yrinic acid a,c-diamide adenosyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Cobinamide/cobalamin adenosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0752</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>2 ATP + 2 cob(II)alamin + reduced [electron-transfer flavoprotein] = 2 adenosylcob(III)alamin + 3 H(+) + oxidized [electron-transfer flavoprotein] + 2 triphosphate</text>
            <dbReference type="Rhea" id="RHEA:28671"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16304"/>
            <dbReference type="ChEBI" id="CHEBI:18036"/>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="2.5.1.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>2 ATP + 2 cob(II)yrinate a,c diamide + reduced [electron-transfer flavoprotein] = 2 adenosylcob(III)yrinate a,c-diamide + 3 H(+) + oxidized [electron-transfer flavoprotein] + 2 triphosphate</text>
            <dbReference type="Rhea" id="RHEA:11528"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18036"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:58503"/>
            <dbReference type="ChEBI" id="CHEBI:58537"/>
            <dbReference type="EC" id="2.5.1.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 2/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the Cob(I)alamin adenosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.17" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314071.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0752"/>
    <dbReference type="EnsemblBacteria" id="ABA04017">
        <property type="protein sequence ID" value="ABA04017"/>
        <property type="gene ID" value="Nwi_0752"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0752"/>
    <dbReference type="eggNOG" id="COG2096">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083486_0_0_5"/>
    <dbReference type="OMA" id="HQACTVV"/>
    <dbReference type="OrthoDB" id="1401910at2"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00233"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008817">
        <property type="term" value="F:cob(I)yrinic acid a,c-diamide adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016030">
        <property type="entry name" value="CblAdoTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036451">
        <property type="entry name" value="CblAdoTrfase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029499">
        <property type="entry name" value="PduO-typ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12213">
        <property type="entry name" value="PTHR12213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01923">
        <property type="entry name" value="Cob_adeno_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89028">
        <property type="entry name" value="SSF89028"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00636">
        <property type="entry name" value="PduO_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cob_adeno_trans" evidence="6">
        <location>
            <begin position="7"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU366026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="190" mass="20643" checksum="430A8B48397839DF" modified="2005-10-11" version="1">MVVLNRIYTRTGDDGTTALGTGERRPKYDLRVGAYGTVDETNAAIGVVRLHLSGAPDLDGMLGRIQNDLFDLGADLAVPQREGKAERLRVLTSQVARLENEIDSLNAGLPDLTSFVLPGGTPAAAYLHLARTICRRAERMMVELAARPGEPVSEAAVRYMNRLSDFLFVASRFMNAMGAGDVLWVPGQNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUQ1</accession>
    <name>Q3SUQ1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">FecR protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0724</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314044.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0724"/>
    <dbReference type="EnsemblBacteria" id="ABA03990">
        <property type="protein sequence ID" value="ABA03990"/>
        <property type="gene ID" value="Nwi_0724"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0724"/>
    <dbReference type="eggNOG" id="COG3712">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050192_0_2_5"/>
    <dbReference type="OMA" id="LDADYHN"/>
    <dbReference type="OrthoDB" id="1381035at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006860">
        <property type="entry name" value="FecR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032623">
        <property type="entry name" value="FecR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012373">
        <property type="entry name" value="Ferrdict_sens_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30273">
        <property type="entry name" value="PTHR30273"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16220">
        <property type="entry name" value="DUF4880"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04773">
        <property type="entry name" value="FecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018266">
        <property type="entry name" value="FecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4880" evidence="3">
        <location>
            <begin position="32"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="FecR" evidence="2">
        <location>
            <begin position="124"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="330" mass="36519" checksum="4D1C82AF9BD2B9A2" modified="2005-10-11" version="1">MAVRERSLPDGGRSRLERRRGFRRHNLDKISREAVAWFAIMHADQVAKSDQLAFRAWLRRDERHRVAYADIERLWSGASELPVVKSRRREKRIAVTRRTFGAGMLAAMAGGGGWVAYQQHPFADYRTSAGERRTVRLADDSSVDMAGATALSVDFSSKLRLVTLHRGEAYFSVAPDTSRPFVVEAAAGRTMAAGTAFNVDYVSDDDVRVTVAQHAVDIRVAAEGARLDAGSQLTYGRESIGPPQAIDPASELGWRDGRLIFISQPFGRVVASLNRWRQGRLMVMESALAVRPVTLIVDLQKSGNILATLEDTLPIRVVNVTPYLTLLFAA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-05-25" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUR0</accession>
    <name>ARLY_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Argininosuccinate lyase</fullName>
            <shortName evidence="1">ASAL</shortName>
            <ecNumber evidence="1">4.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Arginosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argH</name>
        <name type="ordered locus">Nwi_0715</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-(N(omega)-L-arginino)succinate = fumarate + L-arginine</text>
            <dbReference type="Rhea" id="RHEA:24020"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="EC" id="4.3.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.1" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314035.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SUR0"/>
    <dbReference type="SMR" id="Q3SUR0"/>
    <dbReference type="STRING" id="323098.Nwi_0715"/>
    <dbReference type="EnsemblBacteria" id="ABA03981">
        <property type="protein sequence ID" value="ABA03981"/>
        <property type="gene ID" value="Nwi_0715"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0715"/>
    <dbReference type="eggNOG" id="COG0165">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027272_2_3_5"/>
    <dbReference type="OMA" id="KKNPDVF"/>
    <dbReference type="OrthoDB" id="751464at2"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00114"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004056">
        <property type="term" value="F:argininosuccinate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01359">
        <property type="entry name" value="Argininosuccinate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00006">
        <property type="entry name" value="Arg_succ_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029419">
        <property type="entry name" value="Arg_succ_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009049">
        <property type="entry name" value="Argininosuccinate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814">
        <property type="entry name" value="PTHR43814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14698">
        <property type="entry name" value="ASL_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00838">
        <property type="entry name" value="argH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000240742" description="Argininosuccinate lyase">
        <location>
            <begin position="1"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00006"/>
        </source>
    </evidence>
    <sequence length="465" mass="50466" checksum="BA7D664A96EAB955" modified="2005-10-11" version="1">MSNKMWGGRFGEGPDAIMEEINVSIDVDRHLYAQDIAASKVHAEMLAAQGIIAANDAKKIGKGLDTILSEIRDDSFDFKRALEDIHMNVESRLGELIGPSAGRLHTARSRNDQVATDFRLYVRDIIDETDNALATFQHTLATRALEFAGTVMPGFTHLQTAQPVTFGHHLLAYVEMAARDRGRFADARRRLNESPLGAAALAGTSFPIDRHATAKGLGFDRPMANSLDAVSDRDFVLETLSAAAIASTHLSRFAEEMVIWTSPLVGLVRLSDKFTTGSSIMPQKRNPDAAELVRAKTGRVVGALTGLLVVMKGLPLAYQKDMQEDKQGAMEAFSALSLAIRAMTGMVADLVPDEARMKAAAGDGYATATDLADWLVRTLKMPFRDAHHVTGRIVAAASKQGVALHELPLSAMQAVEPRITRDALAVLSVEASVKSRTSYGGTAPKNVRAQAKAWLRRLEKKRTPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUR6</accession>
    <name>Q3SUR6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0709</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314029.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03975">
        <property type="protein sequence ID" value="ABA03975"/>
        <property type="gene ID" value="Nwi_0709"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0709"/>
    <dbReference type="HOGENOM" id="CLU_187779_1_0_5"/>
    <dbReference type="OMA" id="HTEWVYY"/>
    <dbReference type="OrthoDB" id="2071559at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="65" mass="7250" checksum="4F216F40ABE85338" modified="2005-10-11" version="1">MHALLRIVGVITLIAGLGFMGQGFRFYSYPADSILVRHTEWVYYGAAMAVVGFLLIIFSRPSEEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUT0</accession>
    <name>Q3SUT0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase, group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0695</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314017.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0695"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03961">
        <property type="protein sequence ID" value="ABA03961"/>
        <property type="gene ID" value="Nwi_0695"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0695"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_2_0_5"/>
    <dbReference type="OMA" id="RSWENSA"/>
    <dbReference type="OrthoDB" id="948440at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="2">
        <location>
            <begin position="14"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="195"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="367" mass="39911" checksum="963CA1038D906F69" modified="2005-10-11" version="1">MRVLVATDAWHPQVNGVVRTLTSLARSASALGARIEFLTPEGFPSMALPTYPGLRISFPNRGEIARRIEAASPDAIHIATEGPVGWMVRGYCRRRKLPFTTSYTTRFPEYIAARSIIPMSTRSIIPMSTRSIIPMSVSYAVLRRFHAAGAMTMVATSSLRQELSARGFKRLGSWTRGVDTELFTPDHAFELDLPRPVFMTVGRVAVEKNLEAFLSLNLPGSKVVIGDGPQKAELESRYPKARFLGEKTGRELSAHLAAADVFVFPSLTDTFGIVQLEALACGTPVAAFPVTGPLDVIADHPVGALDMNLRSACIRALSISREACRSFALQYSWESCARQFIGNLSALQPSRSSPLRGGLMTQGVAQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUU2</accession>
    <name>Q3SUU2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Binding-protein-dependent transport systems inner membrane component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0683</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314005.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0683"/>
    <dbReference type="EnsemblBacteria" id="ABA03949">
        <property type="protein sequence ID" value="ABA03949"/>
        <property type="gene ID" value="Nwi_0683"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0683"/>
    <dbReference type="eggNOG" id="COG0600">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046113_1_2_5"/>
    <dbReference type="OMA" id="LRWHPGY"/>
    <dbReference type="OrthoDB" id="1694312at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="115"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="177"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="227"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="67"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="268" mass="29429" checksum="72C0F3F510C69CD3" modified="2005-10-11" version="1">MSMAGALSGDRKWRLPRLDGVVQWLLPAAILLVWQVACSTGYVPARVLPAPTEVVLAGWKSLQSGELVQNIWVSFWRAVVGFLIGGGIGFAFGLANGLSPFSDKLTDTTLQMIRNVPHLALIPLVILWFGIEEAAKLFLVALGVFFPVYLNTLHGIRTVDPQLIEMGRVYGMNSGELFRRVIFPGALPSIFVGVRFSLGIMWLTLIVAETIASSSGLGYMAMQAREFMLIDVVVLSILIYALLGKVADSASRFLERITLSWHPAFQKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUU7</accession>
    <name>Q3SUU7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Fumarate reductase/succinate dehydrogenase flavoprotein</fullName>
            <ecNumber evidence="5">1.3.99.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0678</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.3.99.1" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314000.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0678"/>
    <dbReference type="EnsemblBacteria" id="ABA03944">
        <property type="protein sequence ID" value="ABA03944"/>
        <property type="gene ID" value="Nwi_0678"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0678"/>
    <dbReference type="eggNOG" id="COG1053">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014312_8_3_5"/>
    <dbReference type="OMA" id="ARKETRW"/>
    <dbReference type="OrthoDB" id="153138at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037099">
        <property type="entry name" value="Fum_R/Succ_DH_flav-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030664">
        <property type="entry name" value="SdhA/FrdA/AprA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632">
        <property type="entry name" value="PTHR11632"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46977">
        <property type="entry name" value="SSF46977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_2" evidence="3">
        <location>
            <begin position="14"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Succ_DH_flav_C" evidence="4">
        <location>
            <begin position="449"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="578" mass="64583" checksum="54BB1834A1A5E1C9" modified="2005-10-11" version="1">MDEIREGSTEVSCDVLVIGGGTAGPMAAYRAKRRHPNMRVILLEKANVKRSGAIAMGMDGLNNAVIPGYATPEQYTKEITIANDGICDQAPVYKYASRCFEMIQELDRFGIKFQKTENGDFDVKKVHHLGSYVLPMPNGDTVKKALYRQLRREKVLISNRYMATRLLTGRDGQVAGAIAVNTRDAEFLVIRAKTVILCAGAAGRLGLPQSGYLWGTYENAANSGDGYAMAYHAGAGFANLECYQINPLIKDYNGPACAYVAGPFGGYTTNNNGNRFIECDYWSGQMMQEFYNELQSGKGPVFLKLDHLHGDTISEIEAVLHNVERPSRGRFHQNRGTDYRERMIEMHISEIGFCSGHSASGVFVDEFARTTVTGLYAAGDMASVPHNYMLGAFTNGAVAGEHAAGFAEEIDLSDYDLSEVEREKARVLAPTKREDGIPPNQIEYKTRRLVNDYLQPPKVTAKMKLGRMRLAEVREDLENRMVARDAHELMRSLEAASIIDCADMAAAASLFRTESRWGFYHHRVDFPEKNNDEWFCHSILRKQDGRMIVEKRPVAAYIVPIEESERSAYDRQRIRKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUW5</accession>
    <name>Q3SUW5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Helix-turn-helix, Fis-type</fullName>
            <ecNumber evidence="3">2.7.7.49</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0640</name>
        <name evidence="3" type="ordered locus">Nwi_0660</name>
        <name evidence="4" type="ordered locus">Nwi_0865</name>
        <name evidence="5" type="ordered locus">Nwi_0872</name>
        <name evidence="6" type="ordered locus">Nwi_1018</name>
        <name evidence="7" type="ordered locus">Nwi_1299</name>
        <name evidence="8" type="ordered locus">Nwi_1932</name>
        <name evidence="9" type="ordered locus">Nwi_1945</name>
        <name evidence="10" type="ordered locus">Nwi_1964</name>
        <name evidence="11" type="ordered locus">Nwi_2135</name>
        <name evidence="12" type="ordered locus">Nwi_2329</name>
        <name evidence="13" type="ordered locus">Nwi_2665</name>
        <name evidence="14" type="ordered locus">Nwi_2819</name>
        <name evidence="15" type="ordered locus">Nwi_2866</name>
        <name evidence="16" type="ordered locus">Nwi_3086</name>
        <name evidence="17" type="ordered locus">Nwi_3112</name>
    </gene>
    <organism evidence="3 18">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="3 18" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="3">Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0640"/>
    <dbReference type="EnsemblBacteria" id="ABA03907">
        <property type="protein sequence ID" value="ABA03907"/>
        <property type="gene ID" value="Nwi_0640"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03926">
        <property type="protein sequence ID" value="ABA03926"/>
        <property type="gene ID" value="Nwi_0660"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04129">
        <property type="protein sequence ID" value="ABA04129"/>
        <property type="gene ID" value="Nwi_0865"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04136">
        <property type="protein sequence ID" value="ABA04136"/>
        <property type="gene ID" value="Nwi_0872"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04280">
        <property type="protein sequence ID" value="ABA04280"/>
        <property type="gene ID" value="Nwi_1018"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04560">
        <property type="protein sequence ID" value="ABA04560"/>
        <property type="gene ID" value="Nwi_1299"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05192">
        <property type="protein sequence ID" value="ABA05192"/>
        <property type="gene ID" value="Nwi_1932"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05205">
        <property type="protein sequence ID" value="ABA05205"/>
        <property type="gene ID" value="Nwi_1945"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05224">
        <property type="protein sequence ID" value="ABA05224"/>
        <property type="gene ID" value="Nwi_1964"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05390">
        <property type="protein sequence ID" value="ABA05390"/>
        <property type="gene ID" value="Nwi_2135"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05583">
        <property type="protein sequence ID" value="ABA05583"/>
        <property type="gene ID" value="Nwi_2329"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05918">
        <property type="protein sequence ID" value="ABA05918"/>
        <property type="gene ID" value="Nwi_2665"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06072">
        <property type="protein sequence ID" value="ABA06072"/>
        <property type="gene ID" value="Nwi_2819"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06116">
        <property type="protein sequence ID" value="ABA06116"/>
        <property type="gene ID" value="Nwi_2866"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06333">
        <property type="protein sequence ID" value="ABA06333"/>
        <property type="gene ID" value="Nwi_3086"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06359">
        <property type="protein sequence ID" value="ABA06359"/>
        <property type="gene ID" value="Nwi_3112"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0640"/>
    <dbReference type="KEGG" id="nwi:Nwi_0660"/>
    <dbReference type="KEGG" id="nwi:Nwi_0865"/>
    <dbReference type="KEGG" id="nwi:Nwi_0872"/>
    <dbReference type="KEGG" id="nwi:Nwi_1018"/>
    <dbReference type="KEGG" id="nwi:Nwi_1299"/>
    <dbReference type="KEGG" id="nwi:Nwi_1932"/>
    <dbReference type="KEGG" id="nwi:Nwi_1945"/>
    <dbReference type="KEGG" id="nwi:Nwi_1964"/>
    <dbReference type="KEGG" id="nwi:Nwi_2135"/>
    <dbReference type="KEGG" id="nwi:Nwi_2329"/>
    <dbReference type="KEGG" id="nwi:Nwi_2665"/>
    <dbReference type="KEGG" id="nwi:Nwi_2819"/>
    <dbReference type="KEGG" id="nwi:Nwi_2866"/>
    <dbReference type="KEGG" id="nwi:Nwi_3086"/>
    <dbReference type="KEGG" id="nwi:Nwi_3112"/>
    <dbReference type="eggNOG" id="COG2801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2963">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027402_10_0_5"/>
    <dbReference type="OMA" id="RTHERHP"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010921">
        <property type="entry name" value="Trp_repressor/repl_initiator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48295">
        <property type="entry name" value="SSF48295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="132"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA04280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA05192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA05224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA05390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA05583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA05918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABA06072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABA06116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ABA06333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ABA06359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="326" mass="37802" checksum="E4F150ECF4A6B3FA" modified="2005-10-11" version="1">MRYPASEKAEIIRLVEASHLPARRTLDKLGIPRATFYRWYDRYLTGGIEALADHRSRPDRVWNRIPDPIRAEIVELALRETELSPRELAVRFTDEKRYFVSEASVYRLLKAHDLITSPAYIVIKAASEFKDKTTAPNQLWQTDFTYLKITGWGWYYLSTVLDDFSRFIVAWKLCATMRADDVTATLDLALAASGLDQITVAHRPRLLSDNGASYISAELATWLDGKGMKHVRGAPYHPQTQGKIERWHQTLKNRILLENYYLPGDLERQVAAFVEHYNHGRYHESIDNLTPADVYFGRGQTILTERERIKRQTIHQRRLQHHLQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUW9</accession>
    <name>Q3SUW9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Sigma-70 region 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0656</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0656"/>
    <dbReference type="EnsemblBacteria" id="ABA03922">
        <property type="protein sequence ID" value="ABA03922"/>
        <property type="gene ID" value="Nwi_0656"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0656"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_12_3_5"/>
    <dbReference type="OMA" id="HLEPFVF"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="2">
        <location>
            <begin position="2"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="3">
        <location>
            <begin position="90"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="151" mass="17004" checksum="89B273FF2A1AB0EA" modified="2005-10-11" version="1">MRIVRDPQTAEDLAQETYLRAARAAESGPIEHLEAFLYQTARNLALDHLRRQRTRGSVQLDGLPTAEIEKVPANSPTPEGVVIERERLNRFWQALSALPERAQTVVVLSRVEGWQNGQIASHLGVSERTVFNDLKLAMAHCRDAIRRLDEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV11</accession>
    <name>Q3SV11_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Beta-lactamase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0613</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313940.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0613"/>
    <dbReference type="EnsemblBacteria" id="ABA03880">
        <property type="protein sequence ID" value="ABA03880"/>
        <property type="gene ID" value="Nwi_0613"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0613"/>
    <dbReference type="eggNOG" id="COG0491">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048478_2_1_5"/>
    <dbReference type="OMA" id="EFYLAHR"/>
    <dbReference type="OrthoDB" id="733552at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041516">
        <property type="entry name" value="LACTB2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17778">
        <property type="entry name" value="BLACT_WH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="2">
        <location>
            <begin position="42"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="305" mass="33275" checksum="48B5F464B55DD801" modified="2005-10-11" version="1">MSDTEDIPFNRDFPLKPGVAEEVMPGVRRVLCDNPGPFTFTGTVSYIVGRGKVAIIDPGPDSEAHARALLDAVRGETVTHIFVTHTHKDHSPNAARIKAATGARVYAEGPHRASRPRFESEKHKPESGADRGFDPDVRLGDGDGVTGEGWALEAVTTPGHTVNHMAFAWKDRSVLFAGDHVMGWSTSIVAPPDGSMTDYMASLEKLSMRDEHLYFAGHGPEIRDAPRFVRFLARHRRAREVSILHRLAKGEADIATLVRAIYIGLDSRLSGAAGYSVLAHLEDLVARGLVLTDGDPVIEGRYWLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVA6</accession>
    <name>Q3SVA6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">3'(2'),5'-bisphosphate nucleotidase CysQ</fullName>
            <ecNumber evidence="5">3.1.3.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">3'(2'),5-bisphosphonucleoside 3'(2')-phosphohydrolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">3'-phosphoadenosine 5'-phosphate phosphatase</fullName>
            <shortName evidence="5">PAP phosphatase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cysQ</name>
        <name evidence="6" type="ordered locus">Nwi_0518</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Converts adenosine-3',5'-bisphosphate (PAP) to AMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>adenosine 3',5'-bisphosphate + H2O = AMP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.1.3.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell inner membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the inositol monophosphatase superfamily. CysQ family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.7" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313846.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0518"/>
    <dbReference type="EnsemblBacteria" id="ABA03785">
        <property type="protein sequence ID" value="ABA03785"/>
        <property type="gene ID" value="Nwi_0518"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0518"/>
    <dbReference type="eggNOG" id="COG1218">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044118_3_0_5"/>
    <dbReference type="OMA" id="SADCYPR"/>
    <dbReference type="OrthoDB" id="1741160at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008441">
        <property type="term" value="F:3'(2'),5'-bisphosphate nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046855">
        <property type="term" value="P:inositol phosphate dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006790">
        <property type="term" value="P:sulfur compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02095">
        <property type="entry name" value="CysQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006240">
        <property type="entry name" value="CysQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020550">
        <property type="entry name" value="Inositol_monophosphatase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00630">
        <property type="entry name" value="IMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2 5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="93"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA03785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="272" mass="28256" checksum="230B6443527A24E2" modified="2005-10-11" version="1">MPVIDAQSAAALVGLLTDLVAKAGAAVLSVNRDRMKTGGKIDGSPVTDADLAADNVIAEGLARLCPDIPVLSEERAHLATPPYSGSLFVIDPLDGTKEFIAGRNEYTVNLAIVTEGMPLLGIIGAPALGSIWRGLVGRGAERLTDVSDSRAGVAEPIRTRPFPAPNSQWIAAVSRSHADSRSDAFISRRPGAVRKPLGSAVKFCRIAEGCADIYPRLAPTCEWDVAAGHALVTAAGGKVTDESGAPLRFGEAREGFLVPGFIAWGDPSAAPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVE2</accession>
    <name>Q3SVE2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0233</name>
        <name evidence="2" type="ordered locus">Nwi_0482</name>
        <name evidence="3" type="ordered locus">Nwi_0655</name>
        <name evidence="4" type="ordered locus">Nwi_0708</name>
        <name evidence="5" type="ordered locus">Nwi_0852</name>
        <name evidence="6" type="ordered locus">Nwi_1158</name>
        <name evidence="7" type="ordered locus">Nwi_1314</name>
        <name evidence="8" type="ordered locus">Nwi_1395</name>
        <name evidence="9" type="ordered locus">Nwi_1967</name>
        <name evidence="10" type="ordered locus">Nwi_2030</name>
        <name evidence="11" type="ordered locus">Nwi_2059</name>
        <name evidence="12" type="ordered locus">Nwi_2103</name>
        <name evidence="13" type="ordered locus">Nwi_2107</name>
        <name evidence="14" type="ordered locus">Nwi_2371</name>
        <name evidence="15" type="ordered locus">Nwi_2848</name>
        <name evidence="16" type="ordered locus">Nwi_2948</name>
        <name evidence="17" type="ordered locus">Nwi_3007</name>
    </gene>
    <organism evidence="2 18">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="2 18" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="2">Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0233"/>
    <dbReference type="EnsemblBacteria" id="ABA03501">
        <property type="protein sequence ID" value="ABA03501"/>
        <property type="gene ID" value="Nwi_0233"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03749">
        <property type="protein sequence ID" value="ABA03749"/>
        <property type="gene ID" value="Nwi_0482"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03921">
        <property type="protein sequence ID" value="ABA03921"/>
        <property type="gene ID" value="Nwi_0655"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03974">
        <property type="protein sequence ID" value="ABA03974"/>
        <property type="gene ID" value="Nwi_0708"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04116">
        <property type="protein sequence ID" value="ABA04116"/>
        <property type="gene ID" value="Nwi_0852"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04420">
        <property type="protein sequence ID" value="ABA04420"/>
        <property type="gene ID" value="Nwi_1158"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04575">
        <property type="protein sequence ID" value="ABA04575"/>
        <property type="gene ID" value="Nwi_1314"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04656">
        <property type="protein sequence ID" value="ABA04656"/>
        <property type="gene ID" value="Nwi_1395"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05227">
        <property type="protein sequence ID" value="ABA05227"/>
        <property type="gene ID" value="Nwi_1967"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05289">
        <property type="protein sequence ID" value="ABA05289"/>
        <property type="gene ID" value="Nwi_2030"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05317">
        <property type="protein sequence ID" value="ABA05317"/>
        <property type="gene ID" value="Nwi_2059"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05358">
        <property type="protein sequence ID" value="ABA05358"/>
        <property type="gene ID" value="Nwi_2103"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05362">
        <property type="protein sequence ID" value="ABA05362"/>
        <property type="gene ID" value="Nwi_2107"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05625">
        <property type="protein sequence ID" value="ABA05625"/>
        <property type="gene ID" value="Nwi_2371"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06098">
        <property type="protein sequence ID" value="ABA06098"/>
        <property type="gene ID" value="Nwi_2848"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06198">
        <property type="protein sequence ID" value="ABA06198"/>
        <property type="gene ID" value="Nwi_2948"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06257">
        <property type="protein sequence ID" value="ABA06257"/>
        <property type="gene ID" value="Nwi_3007"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0233"/>
    <dbReference type="KEGG" id="nwi:Nwi_0482"/>
    <dbReference type="KEGG" id="nwi:Nwi_0655"/>
    <dbReference type="KEGG" id="nwi:Nwi_0708"/>
    <dbReference type="KEGG" id="nwi:Nwi_0852"/>
    <dbReference type="KEGG" id="nwi:Nwi_1158"/>
    <dbReference type="KEGG" id="nwi:Nwi_1314"/>
    <dbReference type="KEGG" id="nwi:Nwi_1395"/>
    <dbReference type="KEGG" id="nwi:Nwi_1967"/>
    <dbReference type="KEGG" id="nwi:Nwi_2030"/>
    <dbReference type="KEGG" id="nwi:Nwi_2059"/>
    <dbReference type="KEGG" id="nwi:Nwi_2103"/>
    <dbReference type="KEGG" id="nwi:Nwi_2107"/>
    <dbReference type="KEGG" id="nwi:Nwi_2371"/>
    <dbReference type="KEGG" id="nwi:Nwi_2848"/>
    <dbReference type="KEGG" id="nwi:Nwi_2948"/>
    <dbReference type="KEGG" id="nwi:Nwi_3007"/>
    <dbReference type="eggNOG" id="COG3415">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056788_2_3_5"/>
    <dbReference type="OMA" id="AHHRIPR"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA04420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA04656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA05289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA05317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA05358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA05362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABA05625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABA06098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ABA06198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ABA06257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="116" mass="13264" checksum="1CA20825C0E83A8D" modified="2005-10-11" version="1">MGRAYSNDLRERVVRAVVKGGLSRHQAAAQFGVGISTAINWVQRFHETGSVAPSQIGGYRPKKIAGPHREWLLQRCRKDFTVRGLVAELAERGLKVDYRTMWEFVHAVKLSYKKRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVK4</accession>
    <name>SUCC_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Succinate--CoA ligase [ADP-forming] subunit beta</fullName>
            <ecNumber evidence="1">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Succinyl-CoA synthetase subunit beta</fullName>
            <shortName evidence="1">SCS-beta</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">sucC</name>
        <name type="ordered locus">Nwi_0420</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + CoA + succinate = ADP + phosphate + succinyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the succinate/malate CoA ligase beta subunit family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313751.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVK4"/>
    <dbReference type="SMR" id="Q3SVK4"/>
    <dbReference type="STRING" id="323098.Nwi_0420"/>
    <dbReference type="EnsemblBacteria" id="ABA03687">
        <property type="protein sequence ID" value="ABA03687"/>
        <property type="gene ID" value="Nwi_0420"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0420"/>
    <dbReference type="eggNOG" id="COG0045">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037430_0_2_5"/>
    <dbReference type="OMA" id="ITACDEV"/>
    <dbReference type="OrthoDB" id="316012at2"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00558">
        <property type="entry name" value="Succ_CoA_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013650">
        <property type="entry name" value="ATP-grasp_succ-CoA_synth-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="CoA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005809">
        <property type="entry name" value="Succ_CoA_synthase_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815">
        <property type="entry name" value="PTHR11815"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08442">
        <property type="entry name" value="ATP-grasp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001554">
        <property type="entry name" value="SucCS_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="SSF52210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01016">
        <property type="entry name" value="sucCoAbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="chain" id="PRO_1000082143" description="Succinate--CoA ligase [ADP-forming] subunit beta">
        <location>
            <begin position="1"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="1">
        <location>
            <begin position="9"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="53"/>
            <end position="55"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit alpha</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="331"/>
            <end position="333"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit alpha</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00558"/>
        </source>
    </evidence>
    <sequence length="399" mass="42536" checksum="FB42E9D710A4891F" modified="2005-10-11" version="1">MNIHEYQAKALLREFGVPVSRGVPVLQASEAEAAAETLGGPVWVVKSQIHAGGRGKGKFKEASAGEKGGVRLARSIDEVKTFAGQMLGATLVTAQTGPAGKQVNRLYIEEGSDIDKEFYFSALVDRETSRISFVVSTEGGMNIEEVAHKTPEKIVTFSVDPATGVMSHHGRTVARALRLRGDLGKQAEKLVTQLYDAFIARDMAMLEINPLVVTKQGELRVLDAKISFDSNALYRHSDVVALRDESEEDAKEIEASRFDLSYVALDGQIGCMVNGAGLAMATMDIIKLYGMAPANFLDVGGGATKDKVAAAFKIITADPNVKGILINIFGGIMKCDVIADGVVAAVKQVGLNVPLVVRLEGTNVDAGKKIIRESGLNVLPADDLDDAAQKIVKAVKEVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVM5</accession>
    <name>Q3SVM5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0399</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313730.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0399"/>
    <dbReference type="EnsemblBacteria" id="ABA03666">
        <property type="protein sequence ID" value="ABA03666"/>
        <property type="gene ID" value="Nwi_0399"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0399"/>
    <dbReference type="eggNOG" id="COG5330">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056688_0_0_5"/>
    <dbReference type="OMA" id="RTIRFWR"/>
    <dbReference type="OrthoDB" id="750413at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019285">
        <property type="entry name" value="DUF2336"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10098">
        <property type="entry name" value="DUF2336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="377" mass="41119" checksum="4872B1D12D8E9EAD" modified="2005-10-11" version="1">MAEAASTALIAELESAVKGGSSERRLQMLRQVTDLFLSDADRLNENQIGVFDDVLVRLMEQMEARALAQLSTNLSGSSLAPKETVRQLAYHQDASVAVPVLARSARLSEGDLIEIASLRGPQHLLAISHRETLSEGVTDALLKRGDTRVSHTLARNTGARFSERGYAVLVESSEKDDALAEKLGLRLDVPIKVLRELLSRASAAVRERLLKVAPPEMRARIQAAIQSVVEQIASPSLAPVNYAESESMVLALNRAGKLGDQTINRFAIQSEYTHIIAALSLLNSVRIEAIEPLLANPRPDGLIVACKAARLNWSTTHMILRNRPHCRPLTAQEFENGREVFDALSLSSAQRTIRFWSARGSARKADAPDTAVAISDI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVM9</accession>
    <name>AROK_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Shikimate kinase</fullName>
            <shortName evidence="1">SK</shortName>
            <ecNumber evidence="1">2.7.1.71</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aroK</name>
        <name type="ordered locus">Nwi_0395</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + shikimate = 3-phosphoshikimate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13121"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.71"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the shikimate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.71" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313726.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVM9"/>
    <dbReference type="SMR" id="Q3SVM9"/>
    <dbReference type="STRING" id="323098.Nwi_0395"/>
    <dbReference type="EnsemblBacteria" id="ABA03662">
        <property type="protein sequence ID" value="ABA03662"/>
        <property type="gene ID" value="Nwi_0395"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0395"/>
    <dbReference type="eggNOG" id="COG0703">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057607_2_0_5"/>
    <dbReference type="OMA" id="IGKHLFE"/>
    <dbReference type="OrthoDB" id="1392851at2"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00088"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004765">
        <property type="term" value="F:shikimate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00464">
        <property type="entry name" value="SK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00109">
        <property type="entry name" value="Shikimate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031322">
        <property type="entry name" value="Shikimate/glucono_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000623">
        <property type="entry name" value="Shikimate_kinase/TSH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023000">
        <property type="entry name" value="Shikimate_kinase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01202">
        <property type="entry name" value="SKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01128">
        <property type="entry name" value="SHIKIMATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000237898" description="Shikimate kinase">
        <location>
            <begin position="1"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="35"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00109"/>
        </source>
    </evidence>
    <sequence length="214" mass="23443" checksum="C0BF0AED5A1F49A4" modified="2005-10-11" version="1">MTSETVSPSSFGASPETAVVAALAGRSLALVGMMGAGKSTIGRRLAARLRMRFVDADAEIELAAGMPIPEIFETHGEPHFRDGEARVIARLLNGGPIVLATGGGAVLREETRARLSERAVSIWLKADAEVILRRVRRRADRPLLQTADPAATIKRLIAEREPIYRQADITVASRDVPYEWIVDECIGLLHRRLCRGEMTRNAMKDPDEGFDTTQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-26" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVN4</accession>
    <name>SECA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein translocase subunit SecA</fullName>
            <ecNumber evidence="1">7.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">secA</name>
        <name type="ordered locus">Nwi_0390</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving both as a receptor for the preprotein-SecB complex and as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + cellular proteinSide 1 = ADP + phosphate + cellular proteinSide 2.</text>
            <dbReference type="EC" id="7.4.2.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">May bind 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer and homodimer. Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF-YajC and YidC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">Distribution is 50-50.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SecA family.</text>
    </comment>
    <dbReference type="EC" id="7.4.2.8" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313721.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVN4"/>
    <dbReference type="SMR" id="Q3SVN4"/>
    <dbReference type="STRING" id="323098.Nwi_0390"/>
    <dbReference type="EnsemblBacteria" id="ABA03657">
        <property type="protein sequence ID" value="ABA03657"/>
        <property type="gene ID" value="Nwi_0390"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0390"/>
    <dbReference type="eggNOG" id="COG0653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005314_3_0_5"/>
    <dbReference type="OMA" id="MVHYDVQ"/>
    <dbReference type="OrthoDB" id="212453at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008564">
        <property type="term" value="F:protein-exporting ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017038">
        <property type="term" value="P:protein import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18803">
        <property type="entry name" value="SF2_C_secA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01382">
        <property type="entry name" value="SecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004027">
        <property type="entry name" value="SEC_C_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000185">
        <property type="entry name" value="SecA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020937">
        <property type="entry name" value="SecA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011115">
        <property type="entry name" value="SecA_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014018">
        <property type="entry name" value="SecA_motor_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011130">
        <property type="entry name" value="SecA_preprotein_X-link_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044722">
        <property type="entry name" value="SecA_SF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011116">
        <property type="entry name" value="SecA_Wing/Scaffold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036266">
        <property type="entry name" value="SecA_Wing/Scaffold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036670">
        <property type="entry name" value="SecA_X-link_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30612">
        <property type="entry name" value="PTHR30612"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02810">
        <property type="entry name" value="SEC-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07517">
        <property type="entry name" value="SecA_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01043">
        <property type="entry name" value="SecA_PP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07516">
        <property type="entry name" value="SecA_SW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00906">
        <property type="entry name" value="SECA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00957">
        <property type="entry name" value="SecA_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00958">
        <property type="entry name" value="SecA_PP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81767">
        <property type="entry name" value="SSF81767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81886">
        <property type="entry name" value="SSF81886"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00963">
        <property type="entry name" value="secA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01312">
        <property type="entry name" value="SECA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51196">
        <property type="entry name" value="SECA_MOTOR_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0653">Protein transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0811">Translocation</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000320869" description="Protein translocase subunit SecA">
        <location>
            <begin position="1"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="873"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="879"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="913"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="105"/>
            <end position="109"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="525"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="937"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="939"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="948"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="949"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="953" mass="107044" checksum="98E3ADDBEE41D2B6" modified="2005-10-11" version="1">MIGAFARKFFGSANDRRVKGYQSRVKAINALEPEIAALSDDALRARTAEFKQQLANGKTLDDLLVPAFATVREAAKRTLGQRHFDVQLIGGMVLHEGDIAEMKTGEGKTLVATLAVYLNALAGNGVHVVTVNDYLASRDAGWMSQVYSFLGLTTGVIVHGLDDVERKAAYACDITYGTNNEYGFDYLRDNMKYRLEDMVQREHFFAIVDEVDSILIDEARTPLIISGPLDDRSEFYNTIDTFMPNLEKVADYEIDEKQRTVALTEAGMEKIETLLREADLLKGDSLYDVENVSVVHHINQALRAHSLFTRDKDYIVRGGEVVIIDEFTGRMMPGRRYSEGLHQALEAKEHQPVQPENQTLASITFQNYFRMYKKLGGMTGTAATEADELADIYKLEVVEIPTNLPIARLDEDDEVYRTQNEKYAAILAEVERANARMQPVLVGTASIEKSEVLADYLTKHGYKQIDFGDPKSMRKLYAAARAGKPAKLFAVLNARFHEQEAYIVAEAGVPGAITIATNMAGRGTDIKLGGSLEMRAQFETADIADEAEKQAKIAEIKADIERFRGMVLKAEEVVEIEPAKGSKPAKTVTHPGGLYIIGSERHESRRIDNQLRGRAGRQGDPGRSKFFLSLDDDLMRIFGSNRLDTMLQRLGLKDGEAITHPWINKALEKAQQKVEARNFDIRKNLLKYDDVQNDQRKVIFEQRVDLMKNESVAETVADMRHAFVEDLVAKHVPENQYPEQWDVAGLKEELKRVLDVELPVDEWAAEEGIAGEELLSRIESRVDEHMAAKVGQWGPDVMRYVEKTILLQTLDHLWREHLVMLDHLRQVIGLRGYGQRDPLQEYKSEAFSLFESLIAHLREAVTGQLMRVEIVPPEEEQPALPEMEAHKLDPNTGEDEMALAQSSFARASLASEGSEGRSRRDPNDPESWGKVGRNEDCPCGSGKKYKHCHGRYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVN8</accession>
    <name>Q3SVN8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0386</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313717.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0386"/>
    <dbReference type="EnsemblBacteria" id="ABA03653">
        <property type="protein sequence ID" value="ABA03653"/>
        <property type="gene ID" value="Nwi_0386"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0386"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046586_0_3_5"/>
    <dbReference type="OMA" id="PSMWELM"/>
    <dbReference type="OrthoDB" id="1664438at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="2">
        <location>
            <begin position="81"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="289" mass="31755" checksum="FA3159EAA3750619" modified="2005-10-11" version="1">MAPDSSSLPPVLFDRALLRVRQERAAKAGAVPFLLDRVAEDMAERLNAVLRDLQHAADIGTPGDQVRGALSVRVRELARVDLPVDESEAVRLSPDSLDLAVSGLAFQFVNDLPGLLVQIRRALKPDGLLLAATIGGETLTELRQAFAMAEAECEGGISPRVAPFADLRDIGALLQRAGFALPVTDVDRVVVRYGDAFALMRDLRRMGATNVLTARRRTPLRRSTLLRMAQIYHERFTDPDGRIRATFDIVWLSGWAPHESQQKPLQPGSAKARLEDAVRNTSKEKPGRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVS8</accession>
    <name>Q3SVS8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hpr(Ser) kinase/phosphatase</fullName>
            <ecNumber evidence="2">2.7.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0346</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313678.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0346"/>
    <dbReference type="EnsemblBacteria" id="ABA03613">
        <property type="protein sequence ID" value="ABA03613"/>
        <property type="gene ID" value="Nwi_0346"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0346"/>
    <dbReference type="eggNOG" id="COG1493">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052030_2_1_5"/>
    <dbReference type="OMA" id="CTAICQA"/>
    <dbReference type="OrthoDB" id="1753972at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006109">
        <property type="term" value="P:regulation of carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01918">
        <property type="entry name" value="HprK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011104">
        <property type="entry name" value="Hpr_kin/Pase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07475">
        <property type="entry name" value="Hpr_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hpr_kinase_C" evidence="1">
        <location>
            <begin position="12"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="169" mass="17655" checksum="5BD3FFC870EEA774" modified="2005-10-11" version="1">MTGSSGTTIGPTIHGSAVLVGEQAVLIRGPSGAGKSRLAFDLILAGRGGQLPETTLVGDDRLFVTPFRNGLLVRPPSELEGMLEIRGLGIRRCAFVAEAPVGLVIDLDAPDAERLPPPNALRTTILEVELARIPVASGFPPLPIAIAALTTIQGCTDWRPATDCRKKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVZ6</accession>
    <name>Q3SVZ6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0278</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313611.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0278"/>
    <dbReference type="EnsemblBacteria" id="ABA03545">
        <property type="protein sequence ID" value="ABA03545"/>
        <property type="gene ID" value="Nwi_0278"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0278"/>
    <dbReference type="eggNOG" id="ENOG502ZBS3">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_683110_0_0_5"/>
    <dbReference type="OMA" id="AELEMMN"/>
    <dbReference type="OrthoDB" id="781430at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229275" evidence="2">
        <location>
            <begin position="30"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="398" mass="43763" checksum="6B7FF31DBFAD82B2" modified="2005-10-11" version="1">MLSRMIRRCHQIVAGALLLASLAEVPDSAAAPASFAGAMAQAASPAAIADYRRKLKAYQEARAAFEQEAGAYWNAIADKRRIRNAKRRDRQPVSLDDYVLVHPPLYDGPQRPINPEPEEERSPRQRKSIPVVADFLRAAAEQFQFTPQRPRTETDFKRAYASVARAYGLTKEQAVRVYSFETGGNGNHDMQSGLSTSRPGSRAISTAIGYNQLLTTNSVELLAEQGDDLLRALGEKAARSSGPQRAAAERKIAVVKRMVAYARSVPDTWSAHQKLADTPKGWAIHALVLDIDVGPLLQTHKLLTSIIFARNKGYARPLTAAELEMMNLTGDGTGFDMVTMPPVMRERVPTANFFQRRGYERNPVAIRHNTVAKLLAVTNARMDSNSAKPGARELAAAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW42</accession>
    <name>Q3SW42_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">[SSU ribosomal protein S5P]-alanine acetyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.128</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0231</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.128" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313566.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0231"/>
    <dbReference type="EnsemblBacteria" id="ABA03499">
        <property type="protein sequence ID" value="ABA03499"/>
        <property type="gene ID" value="Nwi_0231"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0231"/>
    <dbReference type="eggNOG" id="COG1670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_40_1_5"/>
    <dbReference type="OMA" id="EQFSMIR"/>
    <dbReference type="OrthoDB" id="1501005at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="34"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="194" mass="21963" checksum="084E8AF4DE8FA929" modified="2005-10-11" version="1">MALFRLSSSAPALAPRGHGLFLRAPEMSDFLQWRQLRECSRAYLTPWEPIWPADDLTRAGFRRRLRRYAEDIADDRSYPFIVFRESDGVMLGGITLANVRRGIAQAGTIGYWIGQPHAGQGYMTAALRVLLPALFGELNLHRIEAACIPSNAPSIRVLDKCGFTREGLARRYLRINGIWQDHLLFGVLHDDLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWC6</accession>
    <name>Q3SWC6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Possible transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0147</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03415">
        <property type="protein sequence ID" value="ABA03415"/>
        <property type="gene ID" value="Nwi_0147"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0147"/>
    <dbReference type="eggNOG" id="COG3677">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044348_1_0_5"/>
    <dbReference type="OMA" id="LTKRINH"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024445">
        <property type="entry name" value="Tnp_ISXO2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024442">
        <property type="entry name" value="Transposase_Zn_ribbon"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12762">
        <property type="entry name" value="DDE_Tnp_IS1595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12760">
        <property type="entry name" value="Zn_Tnp_IS1595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01126">
        <property type="entry name" value="DDE_Tnp_IS1595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE_Tnp_IS1595" evidence="1">
        <location>
            <begin position="147"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="328" mass="36860" checksum="81BBCD0208E6F8FC" modified="2005-10-11" version="1">MRSTENVGLMSQHFLLSAKARTLSLAKVARLSDDEAYDAFRLIRWAATDGAPVCPRCECAAVYTYTTRRLFKCKACSHQFSVTSDTIFASRKLPVRDYLLAIAIFVNGAKGHSALQLSRDLDCQYKTAFVMAHKIREALASEAHGAIASGEVEVDGAYFGGYVKPANHKGNRRDRRRIINQNGKRRVVVIMRERGGRTLPFVFKSEDASLVKIAQTVAPGSVIHADEATHWDALHARFLTKRINHSESYSDGDACTNQAESFFSRMRRAEIGVHHRIAGDYLSAYAGEMAWRENNRRISNGEQYLMVADAALQHPVSRQWKGYWQRNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWE5</accession>
    <name>Q3SWE5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">GCN5-related N-acetyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.82</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0128</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Siderophore biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.82" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313465.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0128"/>
    <dbReference type="EnsemblBacteria" id="ABA03396">
        <property type="protein sequence ID" value="ABA03396"/>
        <property type="gene ID" value="Nwi_0128"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0128"/>
    <dbReference type="eggNOG" id="COG1670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_12_0_5"/>
    <dbReference type="OMA" id="DWGWEVE"/>
    <dbReference type="OrthoDB" id="1538300at2"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047663">
        <property type="term" value="F:aminoglycoside 6'-N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019290">
        <property type="term" value="P:siderophore biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019432">
        <property type="entry name" value="Acyltransferase_MbtK/IucB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="SMART" id="SM01006">
        <property type="entry name" value="AlcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="3"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="159" mass="17710" checksum="4A5898B7E44A98FF" modified="2005-10-11" version="1">MAYGFRPMTAADLPTIRRWLDTPHVAEWWGDPAEQFELVSGDLDHPDMAQFIVTDGGRDFAYLQCYRLSDWHTGLGEQPDGTRGLDQFIGEAGMIGRGHGSAFIRSFADRLLIKGVPRIVIDPDPDNARAIRAYEKAGFHRDRLAATPDGPALLMIRDA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWL5</accession>
    <name>IHFB_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integration host factor subunit beta</fullName>
            <shortName evidence="1">IHF-beta</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ihfB</name>
        <name evidence="1" type="synonym">himD</name>
        <name type="ordered locus">Nwi_0058</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein is one of the two subunits of integration host factor, a specific DNA-binding protein that functions in genetic recombination as well as in transcriptional and translational control.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of an alpha and a beta chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial histone-like protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313397.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWL5"/>
    <dbReference type="SMR" id="Q3SWL5"/>
    <dbReference type="STRING" id="323098.Nwi_0058"/>
    <dbReference type="EnsemblBacteria" id="ABA03326">
        <property type="protein sequence ID" value="ABA03326"/>
        <property type="gene ID" value="Nwi_0058"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0058"/>
    <dbReference type="eggNOG" id="COG0776">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105066_2_0_5"/>
    <dbReference type="OMA" id="IAEANPH"/>
    <dbReference type="OrthoDB" id="1847472at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00381">
        <property type="entry name" value="IHF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000119">
        <property type="entry name" value="Hist_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020816">
        <property type="entry name" value="Histone-like_DNA-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010992">
        <property type="entry name" value="IHF-like_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005685">
        <property type="entry name" value="IHF_beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33175">
        <property type="entry name" value="PTHR33175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00216">
        <property type="entry name" value="Bac_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01727">
        <property type="entry name" value="DNABINDINGHU"/>
    </dbReference>
    <dbReference type="SMART" id="SM00411">
        <property type="entry name" value="BHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47729">
        <property type="entry name" value="SSF47729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00988">
        <property type="entry name" value="hip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00045">
        <property type="entry name" value="HISTONE_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <keyword id="KW-0810">Translation regulation</keyword>
    <feature type="chain" id="PRO_1000060623" description="Integration host factor subunit beta">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="101" mass="11415" checksum="7DA82D443CF623A4" modified="2005-10-11" version="1">MIKSELVQRIAEHNPHLYQRDVENIVNAILDEIVAALARGDRVELRGFGAFSVKHRPARAGRNPRTGEHVPVDQKSVPFFKTGKEMRERLNRDNATSEANA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWM4</accession>
    <name>Q3SWM4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PDDEXK_1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0049</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313388.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0049"/>
    <dbReference type="EnsemblBacteria" id="ABA03317">
        <property type="protein sequence ID" value="ABA03317"/>
        <property type="gene ID" value="Nwi_0049"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0049"/>
    <dbReference type="eggNOG" id="COG2887">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3893">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012377_0_0_5"/>
    <dbReference type="OMA" id="ALWWPRF"/>
    <dbReference type="OrthoDB" id="1283891at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014153">
        <property type="entry name" value="Ds_break_AddB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038726">
        <property type="entry name" value="PDDEXK_AddAB-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12705">
        <property type="entry name" value="PDDEXK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02786">
        <property type="entry name" value="addB_alphas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDDEXK_1" evidence="1">
        <location>
            <begin position="769"/>
            <end position="1012"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1053" mass="114038" checksum="B877C22508B44511" modified="2005-10-11" version="1">MRVRNVPSSVPFLRAVITALVDGELIEGFRPRAQPERLAEATLYLPTRRAGRMARDIFLDVLDTDAVILPRIVALGGIDEDELAFAQAVSLPPETLELPPALDGLARRLALAQLIDAWARRLNLGDGEPARAPLVLGGPASTLTLAADLARLMDDMATRGVDWRALDGLVPEALDRYWQITLDFLKIARDYWPAHLQETGRIEPAARRDRLIEAEAARLAAHHVGPVIAAGSTGSMPSTAKLLHAIATLPQGAVVLPGLDTDLDEEAWRLIGGVRDKASGAFITPPAAGHPQFALHGLLARFGIKRSEVKALGFPAPYGREMLASEAMRPAEATAQWHTRLAEPEVADKIAAGLQNLAVIAAANPEMEALGIAVAMREARDLNKTAALVTFDRALARRVMAALGRWNLAFDDSGGDPLMDTPAGIFARLAAETACNGLEPPTLLALLKHPLCRLGRAAGGWSRAIIALELAILRGPRPPPGGKGLADEFARFCDERDQLDRGESSSLHRSEPRAALKPDHLDDISALIAALRDALAPLEGDGASRSADFTLLALQHRQTIESLSCDDEGVAVAFDGRHGRALADAFDDLVDAGERSGLTVRIADYPETFEAAFGDRVVRRPQATSASLRIYGPLEARLTQCDRVILGGLTEGVWPPAPPTDPWLSRPMRHELGLDLPERRIGLSAHDFTQLFGAEEVILSHAAKAAGAPAVASRFLHRLEAVAGATRWTSAKQAGARYIQYAEALDRPSEVTPIAQPAPKPPRTARPTRLSVTAIEDWLRDPYTIYARYILKLLPLEAVDMPLSAADRGSAIHDALGDFTKRYPASLPDDPEDVLRAIGESRFAPLMQRPEARALWWPRFQRIAAWFAEWEPTRRAHLVRIDAEVSGKIEIPIDGDRRFTLSARADRIEHLGGGRFAVLDYKTGSPPSSKQVRLGLSPQLTLESAILRNGGFAGIPPGASVSELVYVRLSGNNPAGEPRPVDLDNGKTATRSPDQAADVALEELTALIRAFDDEQQGYASLDLPMWKARYGVYDDLARIKEWSAAGGPGSEEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWN6</accession>
    <name>Q3SWN6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">D-isomer specific 2-hydroxyacid dehydrogenase</fullName>
            <ecNumber evidence="5">1.1.1.26</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0037</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.26" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313376.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0037"/>
    <dbReference type="EnsemblBacteria" id="ABA03305">
        <property type="protein sequence ID" value="ABA03305"/>
        <property type="gene ID" value="Nwi_0037"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0037"/>
    <dbReference type="eggNOG" id="COG1052">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019796_1_2_5"/>
    <dbReference type="OMA" id="KMKPNCI"/>
    <dbReference type="OrthoDB" id="1638924at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047964">
        <property type="term" value="F:glyoxylate reductase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2-Hacid_dh" evidence="3">
        <location>
            <begin position="18"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="2-Hacid_dh_C" evidence="4">
        <location>
            <begin position="114"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="333" mass="36627" checksum="50AB97AB769066C2" modified="2005-10-11" version="1">MSIKKKPLVVVTRKLPDSVETRMRELFDARLNLDDRPMTPDELIDAVRTADILVTTVTDHINAAVLKQPDCKLKLIAQFGNGVDNIDVVAAHEHGVTVTNTPNVLTEDTADMTMALILAVPRRFIEGAALLTAGGDWPGWSPTWMLGRRLGGKRLGIIGMGRIGQAVARRAHAFGLQIHYHNRKPVAPKIADELGATYWDSLDQMLARMDIISVNCPHTPATFHLLSARRLKLIRKDAYIVNTARGTVIDEDTLTKLIEAGEIAGAALDVFEHEPAVNPRLVRLAKAGKVVLLPHMGSATIEGRVEMGEKVIINIRTFLDNHKPPDRVLPSML</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWP4</accession>
    <name>Q3SWP4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_0029</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NodD regulates the expression of the nodABCFE genes which encode other nodulation proteins. NodD is also a negative regulator of its own expression. Binds flavonoids as inducers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313368.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0029"/>
    <dbReference type="EnsemblBacteria" id="ABA03297">
        <property type="protein sequence ID" value="ABA03297"/>
        <property type="gene ID" value="Nwi_0029"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0029"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_4_5"/>
    <dbReference type="OMA" id="DQALDIC"/>
    <dbReference type="OrthoDB" id="990666at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0536">Nodulation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="5">
        <location>
            <begin position="2"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA03297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="304" mass="33041" checksum="A9316665FFADC24E" modified="2005-10-11" version="1">MVTLRQLRYLAALARHGHFGRAAEACAITQPALSMQIRELEQFLGVTLVERRPGEVMLTEIGREISRRAEDVLASVRDLVDFARHRGRPLTGRLTLGIIPSLAPYLLPRILPALQSQFPDLELELRETQTRLLVEEVKGGALDAAMLALPLGEPDIDTLELFNDLFLLAVPASDPRPCGRRVAARDIDQSRLILLEDGHCLRDQALAFCATIPRGPSSGATGMTFGASSLATVMQMVAGGYGLTLIPQIAADVEARDERVKILRLQDPQPGRCIGLAFRRTSPRRADFAALGEVVKGCVAANGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMV5</accession>
    <name>Q3SMV5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metal-dependent phosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_3139</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316294.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3139"/>
    <dbReference type="EnsemblBacteria" id="ABA06386">
        <property type="protein sequence ID" value="ABA06386"/>
        <property type="gene ID" value="Nwi_3139"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3139"/>
    <dbReference type="eggNOG" id="COG3437">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_92_10_5"/>
    <dbReference type="OMA" id="MQAWALG"/>
    <dbReference type="OrthoDB" id="1094814at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037522">
        <property type="entry name" value="HD_GYP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006675">
        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00277">
        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51832">
        <property type="entry name" value="HD_GYP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD-GYP" evidence="1">
        <location>
            <begin position="176"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="362" mass="39712" checksum="2FA985F47D3A8DE2" modified="2005-10-11" version="1">MTPTTGSRKKRRLLLASDRIDRSSDLCAILQGVADVDTTLTSGIPDRPAGRFAGIVVDINLRSPESVLQVRGKLSAEDYGSIPRLFVLADAFHHGSMQAWALGATDVILRPLDAQAVLQRVRSVFPDDHDGSIASTNALNRGIAAAHAVLVKIFESLPAGVPLSFSDVMEAEQEILTAIKLSSLRDWLATVGRHHTHTYRHCLFVTGFAAAFAQHLGMREDDQRRLARAALLHDVGKAFVPVTILDKPGRLDNEEKDETRRHARLGYDALLRQGGFPSEMLDVVLHHHEMLDGSGYPDGLHGEEISDIVRLTTIVDIHAALVEKRAYRLPLTHAESFATMEEMGGKIDQHLLQAFRPVALGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN01</accession>
    <name>Q3SN01_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Helix-turn-helix protein, CopG</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_3093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316253.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3093"/>
    <dbReference type="EnsemblBacteria" id="ABA06340">
        <property type="protein sequence ID" value="ABA06340"/>
        <property type="gene ID" value="Nwi_3093"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3093"/>
    <dbReference type="eggNOG" id="COG3609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144805_3_1_5"/>
    <dbReference type="OMA" id="KDWVEAQ"/>
    <dbReference type="OrthoDB" id="1897062at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.10.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022789">
        <property type="entry name" value="ParD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038296">
        <property type="entry name" value="ParD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36582">
        <property type="entry name" value="PTHR36582"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03693">
        <property type="entry name" value="ParD_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="SSF47598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02606">
        <property type="entry name" value="antidote_CC2985"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-1277">Toxin-antitoxin system</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="84" mass="9219" checksum="43F3AABD6A246012" modified="2005-10-11" version="1">MATMNVSLPDAMKDWVEAQAETGRYANASDYVRDLIRKDQERNDKIAAMQRFVDEGLNSGAGNRSKDALFAEAVKRAGTPRGNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN37</accession>
    <name>Q3SN37_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bifunctional protein PutA</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="2">Proline dehydrogenase</fullName>
                <ecNumber evidence="2">1.5.5.2</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="2">Proline oxidase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="2">Delta-1-pyrroline-5-carboxylate dehydrogenase</fullName>
                <shortName evidence="2">P5C dehydrogenase</shortName>
                <ecNumber evidence="2">1.2.1.88</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="2">L-glutamate gamma-semialdehyde dehydrogenase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_3055</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Oxidizes proline to glutamate for use as a carbon and nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H2O + L-glutamate 5-semialdehyde + NAD(+) = 2 H(+) + L-glutamate + NADH</text>
            <dbReference type="Rhea" id="RHEA:30235"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="EC" id="1.2.1.88"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a quinone + L-proline = (S)-1-pyrroline-5-carboxylate + a quinol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23784"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.5.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 1/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the proline dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.5.2" evidence="2"/>
    <dbReference type="EC" id="1.2.1.88" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316220.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3055"/>
    <dbReference type="EnsemblBacteria" id="ABA06304">
        <property type="protein sequence ID" value="ABA06304"/>
        <property type="gene ID" value="Nwi_3055"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3055"/>
    <dbReference type="eggNOG" id="COG0506">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4230">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005682_1_0_5"/>
    <dbReference type="OMA" id="PPWNFPV"/>
    <dbReference type="OrthoDB" id="14333at2"/>
    <dbReference type="UniPathway" id="UPA00261">
        <property type="reaction ID" value="UER00373"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003842">
        <property type="term" value="F:1-pyrroline-5-carboxylate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004657">
        <property type="term" value="F:proline dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006561">
        <property type="term" value="P:proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010133">
        <property type="term" value="P:proline catabolic process to glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025703">
        <property type="entry name" value="Bifunct_PutA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005933">
        <property type="entry name" value="Delta1-pyrroline-5-COlate_DH-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024089">
        <property type="entry name" value="PRODH_PutA_dom_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024082">
        <property type="entry name" value="PRODH_PutA_dom_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002872">
        <property type="entry name" value="Proline_DH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01619">
        <property type="entry name" value="Pro_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14850">
        <property type="entry name" value="Pro_dh-DNA_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000197">
        <property type="entry name" value="Bifunct_PutA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="SSF51730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81935">
        <property type="entry name" value="SSF81935"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01238">
        <property type="entry name" value="D1pyr5carbox3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="1 2 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0642">Proline metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Pro_dh-DNA_bdg" evidence="7">
        <location>
            <begin position="59"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Pro_dh" evidence="6">
        <location>
            <begin position="184"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="Aldedh" evidence="5">
        <location>
            <begin position="543"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="759"/>
        </location>
    </feature>
    <feature type="active site" evidence="3 4">
        <location>
            <position position="793"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR000197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000197-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA06304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1001" mass="107717" checksum="C1C890DA8FB5F412" modified="2005-10-11" version="1">MSPQPPPPFSVPYAPDDRTIAKQLLTGARLAPEQETRIDQTARRLIDAIRQNDDPLGGVEDMLREFALSTKEGLALMVLAEALLRVPDAETADRFIEDRLGQGDFIHHETRSSAFLVNASAWALGLSARVIQPGETPQGTIGRLAKRLGVPAIRAATRQAMRLMGSHFVLGETIEAALERTHTHSAGCSRYSFDMLGEGARTAADADRYFDSYAAAIEAIGRGAGDNPLPDRPGISVKLSALHPRYEAVSRGRVMAELVPRAIDLARRARALDLAFTVDAEEADRLELSLDVVAAIAVDRSLAGWSGFGLAVQAYQKRAGAVIDYIDDLAQALNRRMMVRLVKGAYWDTEIKRAQERGLEGYPVFTRKAMTDLNYLACARKLLALRPRLFPQFATHNALTVATLLELTGGESGFEFQRLHGMGEALYAALCKDRHAIACRIYAPVGSHRDLLAYLVRRLIENGANSSFVATASDPDVPPETLLRRPADIIGSADNAGHARIPLPRDLFQPERINSRGIEFGERAAVNRLVTDVATERVSAVPVNYSGPNDVNRAVTTARDGFSTWSRAPATERAAALERAADLLEQRSARFIAWLQREGGKTLDDCVSEVREAADFCRYYAAEGRKLFGDAQPLPGPTGERNTLRLRGRGVFAAISPWNFPLAIFMGQVTAALMAGNAVVAKPAEQTPRIAVEAVRLLHEAGIPVKALHLIQGDGHIGAALVAHRDIAGVVFTGSTEVARSINRVLAGKDGPIVPLIAETGGINAMIADATALPEQVADDVATSAFRSAGQRCSALRLLFVQEDVADRIIEMIVGAARELKIGDPSDPATHVGWVIDDDARKRLDAHIARMTREARVHFAGPAPSSGSFVAPHIFELDDARHLTEEVFGPILHIVRYSSSALDGVLEAIADSGYGLTLGIHSRIDDTVEAVVDRLQVGNVYVNRNMIGAVVGVQPFGGHGLSGTGPKAGGPHYLTRFATEQTVTVNTAAAGGNAALMTGEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN58</accession>
    <name>Q3SN58_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_3033</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316201.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3033"/>
    <dbReference type="EnsemblBacteria" id="ABA06283">
        <property type="protein sequence ID" value="ABA06283"/>
        <property type="gene ID" value="Nwi_3033"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3033"/>
    <dbReference type="eggNOG" id="COG2982">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012870_1_0_5"/>
    <dbReference type="OMA" id="FNGWASV"/>
    <dbReference type="OrthoDB" id="1068066at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007844">
        <property type="entry name" value="AsmA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032712">
        <property type="entry name" value="AsmA_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05170">
        <property type="entry name" value="AsmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13502">
        <property type="entry name" value="AsmA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AsmA" evidence="2">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="AsmA_2" evidence="3">
        <location>
            <begin position="344"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="606"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA06283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="649" mass="67454" checksum="D572C0E1E49C1D63" modified="2005-10-11" version="1">MAKALKIVSAVAASLVAVVLMLLVTGIPSRFVTSLVQDRIERDTGYRIAINGTTGIELWPSPGFALHHVTFDRPGSAATDAQLAVGEVRATLELAGLLSGTLKISELTIDHPTLRAPLLRERTGGGVPSRPDRSEETAGDDTAFPIGRVTVSNGAIVFFDPQTGWEDRIDDVNASVRLGADRRINITGDARPGGRSLTFAIAATPPAGPLGRRNIPTELTLEVSGAPTRQLTARAEVRINRQTLLINGLSGSLDDGKFNGWASVDLASKPLVKVDLDFQKLDLSAAPSEASAPRSGAQPSSEQSWSDAPIDVGGLNYVDAQGTISAAELTIGGARFAPAQVEAALARGVLKASFPNLGVYDGRANGSLSIDTSTGDSPSYALKAEVTGVRALPLLSSLAGFDNVDGRMRATADVRGAGASLRAVMSSLAGTASIDVRDGAIRNLNIAKMIRALTSGTLSGWQERPDQTTDLSQLSATATIVKGQATTNDLYLAGPLVRMTGAGSVDLGAKMLALRVEPKLVMTTEGQGGRADPIGLGIPVVVQGSWSEPRIYPDVAGILDDPGAAYAKLREWGQGLFGSSGTGNSGTSVPHIGETLDTLIRQGLGAGAYPAPAESPAAGKPPEGPPRREAPSPDKPPPIDEIMKQMFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNF7</accession>
    <name>Q3SNF7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Trypsin-like serine protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2934</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316109.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2934"/>
    <dbReference type="EnsemblBacteria" id="ABA06184">
        <property type="protein sequence ID" value="ABA06184"/>
        <property type="gene ID" value="Nwi_2934"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2934"/>
    <dbReference type="eggNOG" id="COG0265">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020120_1_2_5"/>
    <dbReference type="OMA" id="NDTDAFP"/>
    <dbReference type="OrthoDB" id="741829at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001940">
        <property type="entry name" value="Peptidase_S1C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00834">
        <property type="entry name" value="PROTEASES2C"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="SSF50494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="1">
        <location>
            <begin position="260"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="374" mass="39490" checksum="19B9AF6BC6035B7A" modified="2005-10-11" version="1">MITIRPFAALRPILLILIIVGTLLPWCPAAAQIPDLGSGRVPTLAPLVREVTPAVVNISVRGRVKEDNPLYRDPFFRDFFDLPRQLEREVQATGSGVIVDAQRGYILTANHVVAQISTAQITTKDGRRFSAGLIGRDPGTDIAVLQIKRGNNLKAIRLGDSDKLEVGDFVIAVGNPFGLGQTVTSGLVSALGRTGLGKHGYEDFIQTDAPINPGNSGGALINLKGELVGINTAIISPGGGNIGIGFAVPINMARQVMEQIVEYGVVRRGRIGVSVQDLSTVSSEPQATGRSEGALIASVLRGSSAEAAGIRRGDIIVAADGVPIRSAAQLRNKIGLARIGERVQLTFDRRGTLHTVVVEISATRSAPAASSLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNJ8</accession>
    <name>Q3SNJ8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2893</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316073.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2893"/>
    <dbReference type="EnsemblBacteria" id="ABA06143">
        <property type="protein sequence ID" value="ABA06143"/>
        <property type="gene ID" value="Nwi_2893"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2893"/>
    <dbReference type="eggNOG" id="COG2813">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067698_2_0_5"/>
    <dbReference type="OMA" id="NPEWGVR"/>
    <dbReference type="OrthoDB" id="1515497at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010342">
        <property type="entry name" value="DUF938"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20974">
        <property type="entry name" value="PTHR20974"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06080">
        <property type="entry name" value="DUF938"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="223" mass="24747" checksum="9B7F85723ADAF6A4" modified="2005-10-11" version="1">MADNPDIGNDRRPWTADDRLDAAAFHRNHVAIWTVLEKFLRGKTGDVLEAGSGTGQHVVFFARHSPGVTWWPTDLNDHHLRSIAAWTARENLANVRPPRRIDLSNPGQLAEMSERPGALLAVFCANVIHIAPWRVAEGLFAGAAHCLRPDGRLFLYGPFKRDGRHTAPSNEAFDAGLRNTDPEWGVRDIDEVAALAKHNGLTLAEITDMPANNLILTFEREPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNK0</accession>
    <name>Q3SNK0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ErfK/YbiS/YcfS/YnhG</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_2891</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YkuD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316071.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2891"/>
    <dbReference type="MEROPS" id="C82.003"/>
    <dbReference type="EnsemblBacteria" id="ABA06141">
        <property type="protein sequence ID" value="ABA06141"/>
        <property type="gene ID" value="Nwi_2891"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2891"/>
    <dbReference type="eggNOG" id="COG1376">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042399_2_1_5"/>
    <dbReference type="OMA" id="REGFQWQ"/>
    <dbReference type="OrthoDB" id="975147at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071972">
        <property type="term" value="F:peptidoglycan L,D-transpeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16913">
        <property type="entry name" value="YkuD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005490">
        <property type="entry name" value="LD_TPept_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038063">
        <property type="entry name" value="Transpep_catalytic_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03734">
        <property type="entry name" value="YkuD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141523">
        <property type="entry name" value="SSF141523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229176" evidence="4">
        <location>
            <begin position="22"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="YkuD" evidence="5">
        <location>
            <begin position="63"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA06141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="196" mass="21717" checksum="8EA96E5DA6262619" modified="2005-10-11" version="1">MTAFRPLILAVVAMGSLMLSATPGFTQRFDGDQPGLVADDSFQLDPEYRKQVVLYRTTEPPGTIIIQTAERHLYLIQGNGRAIRYGIGVGRAGFQWQGILNITRKAEWPDWTPPPEMIARQPYLPRFMAGGPGNPMGARAMYLGTTVYRIHGTNQPDTIGTAISSGCFRLVNADVIDLYDRIPVGTRVIVRQKPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNR0</accession>
    <name>Q3SNR0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2828</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2828"/>
    <dbReference type="EnsemblBacteria" id="ABA06081">
        <property type="protein sequence ID" value="ABA06081"/>
        <property type="gene ID" value="Nwi_2828"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2828"/>
    <dbReference type="eggNOG" id="ENOG5032VCF">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109781_0_0_5"/>
    <dbReference type="OMA" id="LAIEYEF"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="221" mass="24933" checksum="68059401EC874821" modified="2005-10-11" version="1">MSTQGIEAFQDLTLAPAQGDLNAIRTCLVNHQTPDWVHDADQETELRKYSGGDEDIIQFRYVGSDRPMAALTLWQRENGYAVTNIVPAEQGQLSVSEYNALLQDFVRKVVQPAQREFEFNVNITEPVRQLDSWISKGAAESLRRFSVLANKSTTNSHPNDAERWEQFVVAVHLGGPPLGTDILIQWLVEIDGWDEQSAQKLAIDYEKGISLLETYDRLTRG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNS9</accession>
    <name>PHS_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative pterin-4-alpha-carbinolamine dehydratase</fullName>
            <shortName evidence="1">PHS</shortName>
            <ecNumber evidence="1">4.2.1.96</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">4-alpha-hydroxy-tetrahydropterin dehydratase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Pterin carbinolamine dehydratase</fullName>
            <shortName evidence="1">PCD</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">Nwi_2809</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin = (6R)-L-erythro-6,7-dihydrobiopterin + H2O</text>
            <dbReference type="Rhea" id="RHEA:11920"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15642"/>
            <dbReference type="ChEBI" id="CHEBI:43120"/>
            <dbReference type="EC" id="4.2.1.96"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the pterin-4-alpha-carbinolamine dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.96" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316007.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SNS9"/>
    <dbReference type="SMR" id="Q3SNS9"/>
    <dbReference type="STRING" id="323098.Nwi_2809"/>
    <dbReference type="EnsemblBacteria" id="ABA06062">
        <property type="protein sequence ID" value="ABA06062"/>
        <property type="gene ID" value="Nwi_2809"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2809"/>
    <dbReference type="eggNOG" id="COG2154">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081974_3_2_5"/>
    <dbReference type="OMA" id="WAEKWNH"/>
    <dbReference type="OrthoDB" id="1862336at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008124">
        <property type="term" value="F:4-alpha-hydroxytetrahydrobiopterin dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00434">
        <property type="entry name" value="Pterin_4_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036428">
        <property type="entry name" value="PCD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001533">
        <property type="entry name" value="Pterin_deHydtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599">
        <property type="entry name" value="PTHR12599"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01329">
        <property type="entry name" value="Pterin_4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55248">
        <property type="entry name" value="SSF55248"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000231454" description="Putative pterin-4-alpha-carbinolamine dehydratase">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00434"/>
        </source>
    </evidence>
    <sequence length="101" mass="11367" checksum="90EC6CFBB92518C2" modified="2005-10-11" version="1">MVERFSDEARNTALNELPGWSQVAGREAITRTFTFRDFNEAFGFMARVALVAEKTDHHPEWRNVYRTVEVVLATHDAGGVTERDIRLAEAMNAIARQCGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNU5</accession>
    <name>Q3SNU5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 12">Aspartate-semialdehyde dehydrogenase</fullName>
            <shortName evidence="12">ASA dehydrogenase</shortName>
            <shortName evidence="12">ASADH</shortName>
            <ecNumber evidence="7 12">1.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Aspartate-beta-semialdehyde dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">asd</name>
        <name evidence="14" type="ordered locus">Nwi_2793</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the NADPH-dependent formation of L-aspartate-semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl-4-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-L-aspartate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:24284"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.2.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 2/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 12">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 12">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 12">Belongs to the aspartate-semialdehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.11" evidence="7 12"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315991.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2793"/>
    <dbReference type="EnsemblBacteria" id="ABA06046">
        <property type="protein sequence ID" value="ABA06046"/>
        <property type="gene ID" value="Nwi_2793"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2793"/>
    <dbReference type="eggNOG" id="COG0136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049966_0_1_5"/>
    <dbReference type="OMA" id="CEEEMKM"/>
    <dbReference type="OrthoDB" id="1799040at2"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00016"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00463"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00464"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004073">
        <property type="term" value="F:aspartate-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071266">
        <property type="term" value="P:'de novo' L-methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02121">
        <property type="entry name" value="ASADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012080">
        <property type="entry name" value="Asp_semialdehyde_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005986">
        <property type="entry name" value="Asp_semialdehyde_DH_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012280">
        <property type="entry name" value="Semialdhyde_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02774">
        <property type="entry name" value="Semialdhyde_dhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01296">
        <property type="entry name" value="asd_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 12" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="10 12" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="11 12" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="12" id="KW-0521">NADP</keyword>
    <keyword evidence="12 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Semialdhyde_dh" evidence="13">
        <location>
            <begin position="4"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="12">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="242"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="11"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="39"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="160"/>
            <end position="161"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABA06046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="344" mass="37340" checksum="C6575957F34AF9EC" modified="2005-10-11" version="1">MGYKVALVGATGNVGREMLNILDERKFPADEVVALASRRSVGTEVSYGDKTLKVKALENHDFSDTDICLMSAGGAVSKEWSPKIGAAGAVVIDNSSTWRMDPDVPLIVPEVNADAVTGFTKKNIIANPNCSTAQLVVALKPLHDKAVIQRVVVATYQSVSGAGKDAMDELFAQTKAVYTASELVNKKFPKRIAFNVIPEIDVFMEDGYTKEEWKMMAETKKILDPKIKLTATCVRVPVFIGHSEAVSIEFEKPITADEARGILRNAPGCLVIDKHEPGGYVTPYESAGEDATYISRIREDATVENGLSMWVVSDNLRKGAALNAIQIAECLINRKLIRAKPKAA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-01-10" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNV3</accession>
    <name>LEUC_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-isopropylmalate dehydratase large subunit</fullName>
            <ecNumber evidence="1">4.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Alpha-IPM isomerase</fullName>
            <shortName evidence="1">IPMI</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Isopropylmalate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">leuC</name>
        <name type="ordered locus">Nwi_2785</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of LeuC and LeuD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315983.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SNV3"/>
    <dbReference type="SMR" id="Q3SNV3"/>
    <dbReference type="STRING" id="323098.Nwi_2785"/>
    <dbReference type="EnsemblBacteria" id="ABA06038">
        <property type="protein sequence ID" value="ABA06038"/>
        <property type="gene ID" value="Nwi_2785"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2785"/>
    <dbReference type="eggNOG" id="COG0065">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006714_3_4_5"/>
    <dbReference type="OMA" id="CNMSIEM"/>
    <dbReference type="OrthoDB" id="749418at2"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01583">
        <property type="entry name" value="IPMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01026">
        <property type="entry name" value="LeuC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004430">
        <property type="entry name" value="3-IsopropMal_deHydase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033941">
        <property type="entry name" value="IPMI_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="SSF53732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00170">
        <property type="entry name" value="leuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0432">Leucine biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000076770" description="3-isopropylmalate dehydratase large subunit">
        <location>
            <begin position="1"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="409"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="412"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01026"/>
        </source>
    </evidence>
    <sequence length="468" mass="50689" checksum="4216B9ACD4BFC965" modified="2005-10-11" version="1">MSQPTTLYDKIWNDHLVHEAEDGTCLIYIDRHLVHEVTSPQAFEGLRVAGRKVHAPEKTLAVVDHNVPTTDRSKPNPDPESAEQIAALAQNARDFGIEYYNEFDKRQGIVHVIGPEQGFTLPGTTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLIQKKAKNMRAVVDGKLPDGVTAKDIILAIIGEIGTAGGTGYVLEYAGEAIRALSMEGRMTVCNMSIEGGARAGLIAPDETAYEFLKGRPKAPKGADWDAALRYWETLRSDDGAHFDHEIRLDAAKLPPIVTWGTSPEDVIAVTGKVPDPAAIEDEAKRLSKERALHYMGLLPGTKITDIRLDRVFIGSCTNGRIEDLRAAAKIAEGRTVNGNVSAMVVPGSGLVKEQAEAEGLDKIFTRAGFEWREPGCSMCLAMNPDKLKPDERCASTSNRNFEGRQGFKGRTHLVSPSMAAAAAIAGHFVDIREWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNZ3</accession>
    <name>Q3SNZ3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acetyltransferase, GNAT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2745</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315943.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2745"/>
    <dbReference type="EnsemblBacteria" id="ABA05998">
        <property type="protein sequence ID" value="ABA05998"/>
        <property type="gene ID" value="Nwi_2745"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2745"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_18_7_5"/>
    <dbReference type="OMA" id="WQLAYPA"/>
    <dbReference type="OrthoDB" id="1390922at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13673">
        <property type="entry name" value="Acetyltransf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="5"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="149" mass="16638" checksum="1029B02722280AE9" modified="2005-10-11" version="1">MTVSFQLRPYRASDEDAAIALWQATWQQAYPAIDFAARVSWWRERWRNELVPNAAIVVADQSGELAGFVTIDTAGYLDQLVVAPAHWGGDIANALMLEAKRLSPWGITLLVNADNGRAIRFYARNGFAHAGEDVNPISGRPVNRMEWKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP47</accession>
    <name>Q3SP47_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2691</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05944">
        <property type="protein sequence ID" value="ABA05944"/>
        <property type="gene ID" value="Nwi_2691"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2691"/>
    <dbReference type="eggNOG" id="ENOG50327NK">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_182994_0_0_5"/>
    <dbReference type="OMA" id="RDLGEYM"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="118" mass="12579" checksum="4E72FCABBD49C248" modified="2005-10-11" version="1">MLFSVDHDAVESFSFRDHAPGSGNAGTIMGRTTSTRAVIALAVLSLTVLSLGGCANLERHNPSALSQGDDDAYCQTHGGPQGSAAYTACRKNRDVAATRSDRMERSHRDLAERMLNGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP91</accession>
    <name>Q3SP91_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Diguanylate cyclase/phosphodiesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2647</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2647"/>
    <dbReference type="EnsemblBacteria" id="ABA05900">
        <property type="protein sequence ID" value="ABA05900"/>
        <property type="gene ID" value="Nwi_2647"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2647"/>
    <dbReference type="eggNOG" id="COG5001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_70_50_5"/>
    <dbReference type="OMA" id="RFCCAFQ"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGDEF" evidence="3">
        <location>
            <begin position="236"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="EAL" evidence="2">
        <location>
            <begin position="378"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="658" mass="71962" checksum="7D0B62D4DBC3DB33" modified="2005-10-11" version="1">MPGYLLGRQKVVPYVTATAVAASKSLKVRKFPLSDAMKVGKSMSGAPERSEFSVIAGGSGPTPPDESGTGPNDCGVEAGDPSRVNFLAEVVRLLPTAVTVQDEQGHFVFVNAAAAAQFNVPAGAIVGTGSGALLPSKELNDRVNLARGVLQSGRIRISEELIRTDHTTKTYLTTHEPVRISDRNLMLSSSVDISGQKAVEEELFRCAYFDDLTGLPTRRVIEQHANSLIREGEQPVRFAVAFLDIDNFKHINDYYGHASGDELLIEVARRLSFGLRGTDMLSRISGDEFLLLLNPVQSDAELADYIGFLLQRLKAPFFIEGSELFASASIGVSIYPDHGDSYEVLRQNADTAMYHIKNEKKGGAVIFDSGMQREALTRMEIEQSLRLAILDKRFYCAFQPKVDIRTQQVRGIEALVRLRNDDGVIQAPGTFVDLAIELGLIDELTHLVLAEIMRSIDLIDERFGAGVSISINVAAKQAGNVAFMTSFARALEKTGCPARFVVEVTEDAFVAKTRFQTEILPVLRSLGVGISIDDFGIGYSSLSALADITADEIKIDRSFIVDIHKRPRSQGILKAIESLGDALGMTVIAEGVEKFEELAYLQAATRIKYAQGFYFSEPVFLEDLESDKRAVSKRSTSSIGRPLQENRLTYSRATGYSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPF7</accession>
    <name>Q3SPF7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FAD linked oxidase</fullName>
            <ecNumber evidence="2">1.1.2.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2581</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.2.4" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315785.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2581"/>
    <dbReference type="EnsemblBacteria" id="ABA05834">
        <property type="protein sequence ID" value="ABA05834"/>
        <property type="gene ID" value="Nwi_2581"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2581"/>
    <dbReference type="eggNOG" id="COG0277">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017779_9_2_5"/>
    <dbReference type="OMA" id="TPRTCGE"/>
    <dbReference type="OrthoDB" id="1188552at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004458">
        <property type="term" value="F:D-lactate dehydrogenase (cytochrome) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-linked_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="SSF55103"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="1">
        <location>
            <begin position="50"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="497" mass="52846" checksum="3323D39252D88456" modified="2005-10-11" version="1">MTIMMPASDQAVLDRRDAIVADLRAIVPGEGVIASAAEMLPYESDALTAYRQPPMVVVLPETTEQVSRVLKYCFDNGIKVVPRGSGTSLSGGALPLADAVLLGLGKFKRIREIDFDNRVVVTEPGVTNLAISQAVAHAGFYYAPDPSSQIACSIGGNIAENSGGVHCLKYGMTTNNVLGCELVLMSGEVLRIGGKAPEQAGYDLMGIITGSEGLLGVVTEVTVRILRKPETARALMVGFSQVEAAGECVARVIGAGIIPGGMEMMDRPAIHAAEAFVNAGYPLDVEALLIIELDGSSIEVDELIKRVEAIALGCGSTTCRISGSEAERDLFWAGRKAAFPAVGRISPDYICMDGTIPRGALPRALARIRELSEKYGLGVANVFHAGDGNLHPLILYDANKPGEMDKAEAFGADILRCCVELGGVLTGEHGVGIEKRELMPHMFSEADLSQQQRLKCAFDAQGLLNPGKVFPTLHRCAELGRVHVHGGRLPFPDLPRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPG0</accession>
    <name>Q3SPG0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2578</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2578"/>
    <dbReference type="EnsemblBacteria" id="ABA05831">
        <property type="protein sequence ID" value="ABA05831"/>
        <property type="gene ID" value="Nwi_2578"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2578"/>
    <dbReference type="HOGENOM" id="CLU_1720431_0_0_5"/>
    <dbReference type="OMA" id="KVHHKKA"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005819">
        <property type="entry name" value="H1/H5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00624">
        <property type="entry name" value="HISTONEH5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="152" mass="15793" checksum="382C752C479F2F67" modified="2005-10-11" version="1">MAKKSSKAKGVKAKKSKKKAVAANKKSTKKSVKKSAKTASKKKAVKKKAAKRPAKKSVKKSARKSPGKPAKKSAKKSVKKSAKKSVKRSPGKAAKKPAAKRRASTKKAVAKRAAPKPKATVVPETSWAMPEPAASSYGDADQATSGAGEDGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPH3</accession>
    <name>Q3SPH3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATP phosphoribosyltransferase regulatory subunit</fullName>
            <ecNumber evidence="2">2.4.2.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2565</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.2.17" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2565"/>
    <dbReference type="EnsemblBacteria" id="ABA05818">
        <property type="protein sequence ID" value="ABA05818"/>
        <property type="gene ID" value="Nwi_2565"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2565"/>
    <dbReference type="eggNOG" id="COG3705">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025113_6_0_5"/>
    <dbReference type="OMA" id="YYTGFEF"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003879">
        <property type="term" value="F:ATP phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004516">
        <property type="entry name" value="HisRS/HisZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43707">
        <property type="entry name" value="PTHR43707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001549">
        <property type="entry name" value="His-tRNA_synth"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="tRNA-synt_His" evidence="1">
        <location>
            <begin position="21"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA-synt_His" evidence="1">
        <location>
            <begin position="253"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="390" mass="41726" checksum="3644D93A9A53683B" modified="2005-10-11" version="1">MGFDIMSRRAENPATVSPSRADRLLSSFIQAGYIKAEPAILQPAEPFLDLSGEDIRKSLYLTSDPGGEELCLRPDLTIPVARDYLASVRAGKPAGFCYLGPVFRYRGGRSSEFPQAGIESFGREDRAAADAEMLALGLEAVAAFGIADVDIRTGDVALFTALIDALDLYPVWRRRLMKDFNRKVSLAQDIERLTLASGPGRNEYEGVLAALAGSDRKAALALVTDMMSIAGATIVGGRSLDEIADRFLEQSTLKSGALPHEALTIIKRFLSISGDPDEALGQLRALASDAGLDIHQAIDQVESRTGFMAARGVETNKISFATSFGRGVDYYTGFEFELHLKDNGGEPLVAGGRYDDLMTRLGAAAPIPAVGFSVWIEALTDASPLSGSAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPL8</accession>
    <name>Q3SPL8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Branched-chain-amino-acid aminotransferase</fullName>
            <ecNumber evidence="16">2.6.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">Nwi_2520</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Acts on leucine, isoleucine and valine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>2-oxoglutarate + L-isoleucine = (S)-3-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 16">
            <text>2-oxoglutarate + L-leucine = 4-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:18321"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 16">
            <text>2-oxoglutarate + L-valine = 3-methyl-2-oxobutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24813"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4 15">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6 17">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8 17">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7 17">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9 14">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.42" evidence="16"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315724.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2520"/>
    <dbReference type="EnsemblBacteria" id="ABA05773">
        <property type="protein sequence ID" value="ABA05773"/>
        <property type="gene ID" value="Nwi_2520"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2520"/>
    <dbReference type="eggNOG" id="COG0115">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031922_0_2_5"/>
    <dbReference type="OMA" id="LTEVFAC"/>
    <dbReference type="OrthoDB" id="1275805at2"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00058"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00073"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00062"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052656">
        <property type="term" value="F:L-isoleucine transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052654">
        <property type="term" value="F:L-leucine transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050048">
        <property type="term" value="F:L-leucine:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052655">
        <property type="term" value="F:L-valine transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01557">
        <property type="entry name" value="BCAT_beta_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018300">
        <property type="entry name" value="Aminotrans_IV_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005786">
        <property type="entry name" value="B_amino_transII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033939">
        <property type="entry name" value="BCAT_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11825">
        <property type="entry name" value="PTHR11825"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006468">
        <property type="entry name" value="BCAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="SSF56752"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01123">
        <property type="entry name" value="ilvE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00770">
        <property type="entry name" value="AA_TRANSFER_CLASS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 16 18" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="16" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="12 15" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 16 18" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="13">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00003109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00005072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR006468-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU004519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ABA05773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="370" mass="39968" checksum="4F7AC7A33F752FC4" modified="2005-10-11" version="1">MRASFEKIEGAKSLPFDVQPAAEPTPATVRAEKLANPGFGRVFTDHMAMVSYNQAEGWHGARVAARASFPLDPATAVLHYAQEIFEGLKAYKRDDGGVNLFRPDANARRFRNSAERMAMAPLPETVFIEAIEQLVRIDQDWIPGGEGSLYLRPFMIANEVFLGVKPSAAYIFAVIASPVGSYFKGGPAPISIWVSEDYTRAAVGGTGAVKCGGNYAASLRAQAEAIDRGCDQVVFLDAIERRYVEELGGMNVFFVFDDGSLSTPPLGTILPGITRDSIITLARDSGTPVREEPYTIDQWRSDAASGKLREAFACGTAAVISPIGKVCSAHGDFTINGGAAGPVAMGFRKKLVDIQYGRATDPHRWTRKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPR2</accession>
    <name>Q3SPR2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative bacterioferritin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2476</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2476"/>
    <dbReference type="EnsemblBacteria" id="ABA05729">
        <property type="protein sequence ID" value="ABA05729"/>
        <property type="gene ID" value="Nwi_2476"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2476"/>
    <dbReference type="eggNOG" id="COG2906">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159205_0_0_5"/>
    <dbReference type="OMA" id="GPGCPRT"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007419">
        <property type="entry name" value="BFD-like_2Fe2S-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041854">
        <property type="entry name" value="BFD-like_2Fe2S-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04324">
        <property type="entry name" value="Fer2_BFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fer2_BFD" evidence="1">
        <location>
            <begin position="2"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="85" mass="9000" checksum="AC647797EB9DEF1D" modified="2005-10-11" version="1">MIVCSCNVLTDHDVRSAVTSTDDLRRSPKQVYGCLGCSAECGRCARTIKTIIKEALGACARQCCDGCPHSAAHSKVNTSQAEIIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPT3</accession>
    <name>Q3SPT3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cobyrinic acid a,c-diamide synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2455</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315663.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2455"/>
    <dbReference type="EnsemblBacteria" id="ABA05708">
        <property type="protein sequence ID" value="ABA05708"/>
        <property type="gene ID" value="Nwi_2455"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2455"/>
    <dbReference type="eggNOG" id="COG1192">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037612_5_3_5"/>
    <dbReference type="OMA" id="KFRAPVW"/>
    <dbReference type="OrthoDB" id="1631847at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CbiA" evidence="1">
        <location>
            <begin position="4"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="226" mass="23980" checksum="6C2B3DC20982A5A5" modified="2005-10-11" version="1">MNVIVFASRKGGSGKSTLAAHLAAQVHKASRPCLLVDADPQGSLTLWHKLRGTNEPPIRTANRSVTEIINAAKRDGVEWVFIDTPPNLSAVVDDAIRNATMVVIPARPGVFDVNAVQGTIQSCRSARKPYAVVINGAPAMRDGSESPIVTIAREALAKFRAPVWSGQITNRADLLLALAHGEGAREYDSQGRAASEIGRLWAAIERSVKAIRGAAPSGGTMHKHAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPT7</accession>
    <name>Q3SPT7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2451</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DoxX family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315659.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2451"/>
    <dbReference type="EnsemblBacteria" id="ABA05704">
        <property type="protein sequence ID" value="ABA05704"/>
        <property type="gene ID" value="Nwi_2451"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2451"/>
    <dbReference type="eggNOG" id="COG2259">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058421_2_1_5"/>
    <dbReference type="OMA" id="YFMAHAP"/>
    <dbReference type="OrthoDB" id="2025630at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032808">
        <property type="entry name" value="DoxX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07681">
        <property type="entry name" value="DoxX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="132" mass="14091" checksum="E20EDADA3A2DA3F8" modified="2005-10-11" version="1">MDQVFTKWQPIVLSLLRFMSGLLLMQHGTAKLLGFPSLPMFADLQVASLVGVAGIIELVGGGLLVVGLFTRAAAFITSGMTAVAYFLVHAPKTFMPIANGGELAALYCFVFFYLVFAGGGPISLDKVLFNRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPU8</accession>
    <name>Q3SPU8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNA polymerase sigma factor RpoD</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Sigma-70</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpoD</name>
        <name evidence="11" type="ordered locus">Nwi_2440</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts transiently with the RNA polymerase catalytic core.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the sigma-70 factor family. RpoD/SigA subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315648.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2440"/>
    <dbReference type="EnsemblBacteria" id="ABA05693">
        <property type="protein sequence ID" value="ABA05693"/>
        <property type="gene ID" value="Nwi_2440"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2440"/>
    <dbReference type="eggNOG" id="COG0568">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014793_7_1_5"/>
    <dbReference type="OMA" id="IYAYKDA"/>
    <dbReference type="OrthoDB" id="1565734at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00963">
        <property type="entry name" value="Sigma70_RpoD_SigA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000943">
        <property type="entry name" value="RNA_pol_sigma70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009042">
        <property type="entry name" value="RNA_pol_sigma70_r1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007624">
        <property type="entry name" value="RNA_pol_sigma70_r3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007631">
        <property type="entry name" value="RNA_pol_sigma_70_non-ess"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007127">
        <property type="entry name" value="RNA_pol_sigma_70_r1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042189">
        <property type="entry name" value="RNA_pol_sigma_70_r1_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012760">
        <property type="entry name" value="RNA_pol_sigma_RpoD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028630">
        <property type="entry name" value="Sigma70_RpoD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04546">
        <property type="entry name" value="Sigma70_ner"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03979">
        <property type="entry name" value="Sigma70_r1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00140">
        <property type="entry name" value="Sigma70_r1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04539">
        <property type="entry name" value="Sigma70_r3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00046">
        <property type="entry name" value="SIGMA70FCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02393">
        <property type="entry name" value="RpoD_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00715">
        <property type="entry name" value="SIGMA70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00716">
        <property type="entry name" value="SIGMA70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RNA_pol_sigma70" evidence="9">
        <location>
            <begin position="507"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_sigma70" evidence="10">
        <location>
            <begin position="676"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="6">
        <location>
            <begin position="677"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="217"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Sigma-70 factor domain-2" evidence="6">
        <location>
            <begin position="483"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Sigma-70 factor domain-3" evidence="6">
        <location>
            <begin position="562"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Sigma-70 factor domain-4" evidence="6">
        <location>
            <begin position="651"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="434"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Interaction with polymerase core subunit RpoC" evidence="6">
        <location>
            <begin position="507"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA05693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="717" mass="80373" checksum="C1D6E39107F4DD76" modified="2005-10-11" version="1">MASKAKTLQVREKEKDDKAADAPEKDTADAPSPLLDLSDAAVKKMIKQAKKRGFVTFDQLNEVLPSDTTSPEQIEDIMSMLSDMGINVSESEDADSEDENKEEADEETDNELVEVTQKAVTEVKKSEPGERTDDPVRMYLREMGTVELLSREGEIAIAKRIEAGREAMIAGLCESPLTFQAIIIWRDELNEGKIFLRDIIDLEATYAGPEAKSSMNPALLGAPGGPGGQPLSADASDGASGEGGAEATVNAPPSAPPSPTPFRAARAAPAGAADREEKIPGAAAEGEMEEDDFDNQMSLAAIEAELKPKVVETFDKIADSYKKLRRLQEADISNQLQNESLSPNQERRYKKLKDEIIVEVKSLRLNQARIDSLVEQLYDINKRLVSFEGRLLRLGDSHGVAREDFLRNYQGSELDPRWLNRVSKLSAKGWKNFVHHEKDRIKELRGEIQQLAALTGLEIGEFRKIVQGVQKGEREARQAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKIVRTSRQMLNEIGREPTPEELAEKLGMPLEKVRKVLKIAKEPLSLETPVGDEEDSHLGDFIEDKNAVLPIDAAIQSNLRETTTRVLASLTPREERVLRMRFGIGMNTDHTLEEVGQQFSVTRERIRQIEAKALRKLKHPSRSRKLRSFLDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPX1</accession>
    <name>Q3SPX1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Histidine triad (HIT) protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2416</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2416"/>
    <dbReference type="EnsemblBacteria" id="ABA05670">
        <property type="protein sequence ID" value="ABA05670"/>
        <property type="gene ID" value="Nwi_2416"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2416"/>
    <dbReference type="eggNOG" id="COG0537">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056776_3_3_5"/>
    <dbReference type="OMA" id="AMRTIWK"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01277">
        <property type="entry name" value="HINT_subgroup"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039384">
        <property type="entry name" value="HINT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001310">
        <property type="entry name" value="Histidine_triad_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23089">
        <property type="entry name" value="PTHR23089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00332">
        <property type="entry name" value="HISTRIAD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="SSF54197"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="2">
        <location>
            <begin position="10"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="1">
        <location>
            <begin position="102"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="146" mass="15860" checksum="E48B10E32A645A17" modified="2005-10-11" version="1">MMPAYDDQNIFAKILRGELPCFKVYENAHTFAFLDIMPRTPGHTLVIPKAPARGILDISADDLAEVARTSKTIAVAAMKAFDAEGIIIQQFSEAVSGQVVFHLHMHVVPMKSGVALRPPQSRQEDATVLADHAARLIAALGSSREP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPZ3</accession>
    <name>Q3SPZ3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nucleotidyl transferase</fullName>
            <ecNumber evidence="3">2.7.7.13</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2394</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.13" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315609.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2394"/>
    <dbReference type="EnsemblBacteria" id="ABA05648">
        <property type="protein sequence ID" value="ABA05648"/>
        <property type="gene ID" value="Nwi_2394"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2394"/>
    <dbReference type="eggNOG" id="COG0517">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1208">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045375_0_0_5"/>
    <dbReference type="OMA" id="TMCVREY"/>
    <dbReference type="OrthoDB" id="1557977at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004475">
        <property type="term" value="F:mannose-1-phosphate guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="63"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="346" mass="38537" checksum="5000A2E7021E6097" modified="2005-10-11" version="1">MSALFVVTEEIPLLEALRRIDEGNLQLAVVERDGKIVGTVTDGDVRRALLNGVGLDTPVNEVMNRNPVVAPAGISNAAALTLMRRRSIHQLPIVDDHGKVIEIKLIDDLAAASQADHWVVLMAGGLGSRLRPLTDDLPKPLIKVGNKPILETVLNGFIKSGFGKFFISVNYKAEMIREYFGDGSAWGVEIDYLVESDRLGTAGALSLIPERPTRPFFVMNGDLLTTVNFEQMLKYHLEHQAFTTICVREHAITVPFGVVDFEDHRILGIREKPTQKFFVNAGVYLLDPEVLDYIGATEAVDMPTLIERTIERGKRSVAFPLREYWIDVGRLDDLQKASDEFQRIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ30</accession>
    <name>Q3SQ30_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase IS3/IS911</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1298</name>
        <name evidence="3" type="ordered locus">Nwi_2357</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="3">Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1298"/>
    <dbReference type="EnsemblBacteria" id="ABA04559">
        <property type="protein sequence ID" value="ABA04559"/>
        <property type="gene ID" value="Nwi_1298"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05611">
        <property type="protein sequence ID" value="ABA05611"/>
        <property type="gene ID" value="Nwi_2357"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1298"/>
    <dbReference type="KEGG" id="nwi:Nwi_2357"/>
    <dbReference type="eggNOG" id="COG2963">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027402_36_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002514">
        <property type="entry name" value="Transposase_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010921">
        <property type="entry name" value="Trp_repressor/repl_initiator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01527">
        <property type="entry name" value="HTH_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48295">
        <property type="entry name" value="SSF48295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="66"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="109" mass="12349" checksum="E16B8EC5B024110C" modified="2005-10-11" version="1">MKDIRRATRRQFSAEEKIRIVLEGVRGEESIAELCRREGIASSMYYGWSKEFLDAGKRRLAGDTARAATSDEVKELRREAQALKEAVADLTLENRLLKKSMLADGEDDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ72</accession>
    <name>Q3SQ72_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2315</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315534.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2315"/>
    <dbReference type="EnsemblBacteria" id="ABA05569">
        <property type="protein sequence ID" value="ABA05569"/>
        <property type="gene ID" value="Nwi_2315"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2315"/>
    <dbReference type="eggNOG" id="ENOG5033GUS">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_166118_0_0_5"/>
    <dbReference type="OMA" id="MAIWIGH"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="120" mass="12777" checksum="7DC2131AE96F6D14" modified="2005-10-11" version="1">MTGDHRASASMLVWICSGFLAWGAAFAALYSVQALGCGFDWDEITIFGSIDAHRLLLIALFLAALFASAVLARLCARLPHRVRADDTARFLGNVAKLSAWAALAATLVSLGPVLFTTACY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ96</accession>
    <name>Q3SQ96_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2291</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05545">
        <property type="protein sequence ID" value="ABA05545"/>
        <property type="gene ID" value="Nwi_2291"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2291"/>
    <dbReference type="HOGENOM" id="CLU_2058907_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="119" mass="12758" checksum="5357338D8ACC8456" modified="2005-10-11" version="1">MQKRLRSKSGSRDSVRKRNGPGSGQPGRLTPPCDASSARFSQIDSPSFMETRLTRKFVAEAASLAVFGAGLAGVYILAKVLKGDVADLTIALIATVIFVVSYVLGQWVKRTIDPPHAVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQA2</accession>
    <name>Q3SQA2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, TetR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2285</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315506.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2285"/>
    <dbReference type="EnsemblBacteria" id="ABA05539">
        <property type="protein sequence ID" value="ABA05539"/>
        <property type="gene ID" value="Nwi_2285"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2285"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091245_0_0_5"/>
    <dbReference type="OMA" id="KRCFARH"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023772">
        <property type="entry name" value="DNA-bd_HTH_TetR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01081">
        <property type="entry name" value="HTH_TETR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="9"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="233" mass="25282" checksum="3D9214E483A19C5A" modified="2005-10-11" version="1">MSSLRMTGDLRRELILSAAKQCFARNGFAGTTTRSVAAAASISEALLFKHFPTKAALYAEILAEACEADPALHRLLDREPSTETLVILVRGMVRHFLNVSASPDQQEAQRLRLMVSSQLDDGEFARLLYEKIRNLIGGAFTASLEQAIAKGDAEPVGRDPLNLFWFAHHTVLMLVLSRLPSVPALSYGGGPDLERQISEFILRGIGLNAAAIALHLDREPVFNAAQTSAAESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQB4</accession>
    <name>Q3SQB4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2273</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315494.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2273"/>
    <dbReference type="EnsemblBacteria" id="ABA05527">
        <property type="protein sequence ID" value="ABA05527"/>
        <property type="gene ID" value="Nwi_2273"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2273"/>
    <dbReference type="eggNOG" id="ENOG5032MJH">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145244_5_0_5"/>
    <dbReference type="OMA" id="IDQGDFT"/>
    <dbReference type="OrthoDB" id="1982179at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229125" evidence="1">
        <location>
            <begin position="22"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="85" mass="9368" checksum="C1917B5A62F0BEAB" modified="2005-10-11" version="1">MSKILLSSALLLSFGSTLAMAQVQRQPQLQPHSGTEAEHRACVRDVSRFCRAVMDQGDLTVLACLQQYRPKISKACNQVLLSHGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQC9</accession>
    <name>Q3SQC9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Elongator protein 3/MiaB/NifB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_2258</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315480.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2258"/>
    <dbReference type="EnsemblBacteria" id="ABA05512">
        <property type="protein sequence ID" value="ABA05512"/>
        <property type="gene ID" value="Nwi_2258"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2258"/>
    <dbReference type="eggNOG" id="COG4277">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033784_0_0_5"/>
    <dbReference type="OMA" id="GYIHLKA"/>
    <dbReference type="OrthoDB" id="940969at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023874">
        <property type="entry name" value="DNA_rSAM_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21180:SF9">
        <property type="entry name" value="PTHR21180:SF9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01102">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03916">
        <property type="entry name" value="rSAM_link_UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="414" mass="46409" checksum="F2A5E506B4A716C7" modified="2005-10-11" version="1">MNLQRKLEILADAAKYDASCASSGTELRDSRDGKGLGSTAPGMGICHSYAPDGRCISLLKVLLTNACNYDCLYCVNRASSNVPRARFTIDEVVRLTIDFYRRNYIEGLFLSSGIIRSADYTMEQVVAVARKLREEHRFRGYIHLKTIPDANDALITEAGKYADRLSINIEMPEEDSLAKLAPEKDARAIRRTMGRLRLKLDEAKEARKADATARVRAPRFAPAGQTTQMIVGADAASDQTILSTSANLYGSYRLKRVYYSAFSPIPDASRALPLSAPPLMREHRLYQADWLMRFYGFDISEIVDATNPMLPLDIDPKLAWALRHRDRFPLDINRASREELLRVPGFGTKAVDRILSARRYTSIRAADLSRLHVPRNKALPFIVLADHRQSPKALESMTLAEQLKPKAVQLGFGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQF8</accession>
    <name>Q3SQF8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Peptidase M20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2229</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315451.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2229"/>
    <dbReference type="EnsemblBacteria" id="ABA05483">
        <property type="protein sequence ID" value="ABA05483"/>
        <property type="gene ID" value="Nwi_2229"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2229"/>
    <dbReference type="eggNOG" id="COG0624">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029469_2_1_5"/>
    <dbReference type="OMA" id="HITIPGF"/>
    <dbReference type="OrthoDB" id="275025at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="M20_dimer" evidence="2">
        <location>
            <begin position="205"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="468" mass="50926" checksum="C602E044A87897A9" modified="2005-10-11" version="1">MSGTSQLQTVLDHIDADFDNSLERLFALLRIKSISADPAFVDDCKAAADHLAADIATLNFASEVRPTAGHPAIVAKSNGNTGTRPHVLFYGHYDVQPVDPLDLWHRTPFDPVVTNHADGRKIIVARGAEDDKGQLMTFVEACRAWLSVTGSLPLDVTILIEGEEEVGSKNFVPFLEQNKKDLAADFALVCDTGMWDPDTPAITTSLRGLVYEEVIIKAANRDLHSGLFGGGAQNPIRILTRILGDLHDDDGRIAIRGFYDDVKDLPPQIRAQWAELDLTPDRFLKPIGLSIPAGEKDRLLIEQLLSRPTCDINGIVGGYTGEGSKTVIPAQASAKVSFRLVEGQQPGRIRDAFRAFVKERVPADCSVAFLDHAGAPAIALDWNMKPLAAAKQALTEEWNKEALLIGCGASIPIVADFKTSLGLDTVMVGFGLEDDNIHSPNEKYDLKSFHKGIRSWARILAALAEAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQH5</accession>
    <name>Q3SQH5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Amino acid/amide ABC transporter ATP-binding protein 2, HAAT family</fullName>
            <ecNumber evidence="7">3.6.3.25</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2212</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in beta-(1--&gt;2)glucan export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.25" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315435.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2212"/>
    <dbReference type="EnsemblBacteria" id="ABA05466">
        <property type="protein sequence ID" value="ABA05466"/>
        <property type="gene ID" value="Nwi_2212"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2212"/>
    <dbReference type="eggNOG" id="COG0410">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_5"/>
    <dbReference type="OMA" id="IMMQKIM"/>
    <dbReference type="OrthoDB" id="1475841at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="9"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="41"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="247" mass="26760" checksum="EB35C20E207CA06B" modified="2005-10-11" version="1">MNAGATPLLAIRGLRAAYGKIEALKGIDLEIQAGEIVALIGANGAGKSTLMMTIFGRPRARAGQILFDGQDISSMPTHEVARLRIAQSPEGRRIFPRMTVAENLQMGADATPGSKAERAASLDRVLKLFPRLQERMTQRGGTLSGGEQQMLAIGRALMSRPRLLLLDEPSLGLAPLITRQIFSAIRALNNEDHLTVLIVEQNANHALRLAHRGYVMVNGLITMSGEGMELLQRPEIRAAYLEGGRHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQN6</accession>
    <name>Q3SQN6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ppx/GppA phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2151</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315374.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2151"/>
    <dbReference type="EnsemblBacteria" id="ABA05405">
        <property type="protein sequence ID" value="ABA05405"/>
        <property type="gene ID" value="Nwi_2151"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2151"/>
    <dbReference type="eggNOG" id="COG0248">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025908_0_0_5"/>
    <dbReference type="OMA" id="IGCVRMT"/>
    <dbReference type="OrthoDB" id="1862004at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003695">
        <property type="entry name" value="Ppx_GppA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02541">
        <property type="entry name" value="Ppx-GppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ppx-GppA" evidence="1">
        <location>
            <begin position="57"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="355" mass="37888" checksum="02C7F48394D2C7C5" modified="2005-10-11" version="1">MDDDIQRCVDAGTGSRDSFRGVEAAMTAASAIRPSPSADAAVYAALDLGTNNCRLLIASPASGSFRVIDSFSRIVRLGEGISSTGYISEAAIERTIAALAICRDKIRARKAGRLRLIATEACRAASNAESFRSRVAAATGIQLEVVDRETEAALAVIGCSPLVHPKASGAILFDIGGGSTELVRIERRREGDLSTRAWISLPVGVVTLAECFGGHDITPQSYASMVQEVAGHIATFAEAYGRDLYGMHLLGTSGTVTTLGGLHLGLARYDRRKVDGIWMNDIEVTQAIARLCGMDYQKRVANGCIGIERADLVLAGCAILDAIRRAFPLPRLRVADRGLREGMLVEMMREDGAFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQU1</accession>
    <name>Q3SQU1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Conjugal transfer protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_2093</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315328.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2093"/>
    <dbReference type="EnsemblBacteria" id="ABA05350">
        <property type="protein sequence ID" value="ABA05350"/>
        <property type="gene ID" value="Nwi_2093"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2093"/>
    <dbReference type="eggNOG" id="COG3701">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076026_0_0_5"/>
    <dbReference type="OMA" id="FVNALNW"/>
    <dbReference type="OrthoDB" id="1214981at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16425">
        <property type="entry name" value="TrbF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035658">
        <property type="entry name" value="TrbF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007430">
        <property type="entry name" value="VirB8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04335">
        <property type="entry name" value="VirB8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="VirB8" evidence="5">
        <location>
            <begin position="23"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="228" mass="25650" checksum="4DFCF01B3464945C" modified="2005-10-11" version="1">MNLFRRPSVRYARTPEPETPYLKAAQVWDERIGSARVQAKNWRLMAFGSLALAGSFAAALVWQSTQGTVVPWVVQVDKFGEAQAVASAVSDYRPTDPQIAWHLARFIEQVRSIPADPVIVRQNWLRAYDYTTDRGAIALNDYARANDPFTKVGKQQISVEISSVIRASPDSFRIAWLERRYENGQLSGTERWTAILTVVIQAPRTADKLRANPLGVFVNAINWSKEMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQY3</accession>
    <name>Q3SQY3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Probable FecR, iron siderophore sensor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2050</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2050"/>
    <dbReference type="EnsemblBacteria" id="ABA05308">
        <property type="protein sequence ID" value="ABA05308"/>
        <property type="gene ID" value="Nwi_2050"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2050"/>
    <dbReference type="eggNOG" id="COG3712">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050192_0_1_5"/>
    <dbReference type="OMA" id="ARYTEQG"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006860">
        <property type="entry name" value="FecR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032623">
        <property type="entry name" value="FecR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012373">
        <property type="entry name" value="Ferrdict_sens_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30273">
        <property type="entry name" value="PTHR30273"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16220">
        <property type="entry name" value="DUF4880"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04773">
        <property type="entry name" value="FecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018266">
        <property type="entry name" value="FecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4880" evidence="3">
        <location>
            <begin position="28"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="FecR" evidence="2">
        <location>
            <begin position="124"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="329" mass="35762" checksum="E19D888DEE9B22D6" modified="2005-10-11" version="1">MTRPAVEWLVAMTPKDRQQHESDIALSDAAIDWLVRLKSGQATNDDHAAFAAWRGQSMEHELAAREAEAVWHGVGVAGDRARTLERKERRAKVTRRAVLGGGTLAVAGFVLAGSGVIGPRAFADHVTGVGEQRTVGLADGSSVFLNASSGFSVKFSAHERRLSLLEGQATFTVAHDTARPFIVEAGDGQTRAVGTVFDIDIRAGEVVVTVVEGVVAITTDQSPDPVVARVDQRVRYLPRGRPSGPESADAYIETAWRRGKLIFNRRPLADVVADIERYRRGRIVLMGDGLRSLEVTGVFDLNDPEAILHTIEDTLPVQMTRLPFVTIVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR06</accession>
    <name>Q3SR06_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2026</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315271.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2026"/>
    <dbReference type="EnsemblBacteria" id="ABA05285">
        <property type="protein sequence ID" value="ABA05285"/>
        <property type="gene ID" value="Nwi_2026"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2026"/>
    <dbReference type="HOGENOM" id="CLU_2220412_0_0_5"/>
    <dbReference type="OMA" id="HEQQNDS"/>
    <dbReference type="OrthoDB" id="2228131at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229216" evidence="2">
        <location>
            <begin position="28"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="106" mass="10380" checksum="6E7EE4D9F8A72B72" modified="2005-10-11" version="1">MEHTQMNLRLLAATAAVTAFGIAGAQAQQPSPYGQTQTQQNDSVRGSAGASGSPHTQKVHPRGSAKGTVGAAPGRGNVDAGAAGSMDAAPPPAGINGAPRPGGPMR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRB6</accession>
    <name>RECO_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA repair protein RecO</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Recombination protein O</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">recO</name>
        <name type="ordered locus">Nwi_1915</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in DNA repair and RecF pathway recombination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RecO family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="ABA05175" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05175.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315171.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRB6"/>
    <dbReference type="SMR" id="Q3SRB6"/>
    <dbReference type="STRING" id="323098.Nwi_1915"/>
    <dbReference type="EnsemblBacteria" id="ABA05175">
        <property type="protein sequence ID" value="ABA05175"/>
        <property type="gene ID" value="Nwi_1915"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1915"/>
    <dbReference type="eggNOG" id="COG1381">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086029_0_0_5"/>
    <dbReference type="OrthoDB" id="1758685at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00201">
        <property type="entry name" value="RecO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022572">
        <property type="entry name" value="DNA_rep/recomb_RecO_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003717">
        <property type="entry name" value="RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042242">
        <property type="entry name" value="RecO_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33991">
        <property type="entry name" value="PTHR33991"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02565">
        <property type="entry name" value="RecO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11967">
        <property type="entry name" value="RecO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="SSF57863"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00613">
        <property type="entry name" value="reco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000264827" description="DNA repair protein RecO">
        <location>
            <begin position="1"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="262" mass="28447" checksum="C229A01F1C0DE162" modified="2006-12-12" version="2">MEWTDDGIVLGVRRHGESSAIVELLTRTHGRHLGLVRGGAGSRMRPLLQPGNSVSALWRARLDEHLGTYALEGTRMRAASLLASSHATYGVTHLAALARLLPERDPHEVIFAMLERTLDDFDRAGGAAIHLIRFELAMLSELGFGLDLSACAATGATGDLIYVSPKSGGAVSRQAGEPWRDRLLPLPPFLRVTHDEQDGWSEQDLLDGFELTGRFLLRHVLEPRGQGHSDARAGFINAVVRKRTAAIADGARVMAELNPPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRC1</accession>
    <name>Q3SRC1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RDD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_1910</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315166.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1910"/>
    <dbReference type="EnsemblBacteria" id="ABA05170">
        <property type="protein sequence ID" value="ABA05170"/>
        <property type="gene ID" value="Nwi_1910"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1910"/>
    <dbReference type="eggNOG" id="COG1714">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116272_1_0_5"/>
    <dbReference type="OMA" id="WRAYSGV"/>
    <dbReference type="OrthoDB" id="1974702at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010432">
        <property type="entry name" value="RDD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06271">
        <property type="entry name" value="RDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="39"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="120"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="RDD" evidence="6">
        <location>
            <begin position="35"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="179" mass="19768" checksum="1D29A5E20940AEDC" modified="2005-10-11" version="1">MPDGDFDNAPGGDSAGFQRGSFDPWMQPELFRGVLVRRFFAFLIDLLVLSIPVIIGVIFIAMLGIITLGFGWALFWLVSPASLIWAVFYYGASLGGRHSATIGMRAMGLEMRTWSGERSYFVLGAMHAILFWISVSIFTPLILLVGLFNNRRRMLHDMLLGTVIINSDLATAAQPSGSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRC2</accession>
    <name>Q3SRC2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Delta-aminolevulinic acid dehydratase</fullName>
            <ecNumber evidence="4 11">4.2.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Nwi_1909</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>2 5-aminolevulinate = H(+) + 2 H2O + porphobilinogen</text>
            <dbReference type="Rhea" id="RHEA:24064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="4.2.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the ALAD family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.24" evidence="4 11"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315165.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1909"/>
    <dbReference type="EnsemblBacteria" id="ABA05169">
        <property type="protein sequence ID" value="ABA05169"/>
        <property type="gene ID" value="Nwi_1909"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1909"/>
    <dbReference type="eggNOG" id="COG0113">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035731_0_0_5"/>
    <dbReference type="OMA" id="YQMDYAN"/>
    <dbReference type="OrthoDB" id="677575at2"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00318"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004655">
        <property type="term" value="F:porphobilinogen synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001731">
        <property type="entry name" value="ALAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030656">
        <property type="entry name" value="ALAD_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458">
        <property type="entry name" value="PTHR11458"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00490">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001415">
        <property type="entry name" value="Porphbilin_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00144">
        <property type="entry name" value="DALDHYDRTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01004">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00169">
        <property type="entry name" value="D_ALA_DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="7 11 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="9">
        <location>
            <position position="220"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="9">
        <location>
            <position position="274"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA05169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="352" mass="38521" checksum="94C07540D8A7B161" modified="2005-10-11" version="1">MAIKYGRPIEMRDGPRRDAATAAPLDLVARLRRNRKAEWARRLVRESVLTTDDLIWPLFVADGADRRTPVPSMPGVDRLSVDECVRDAERALELNIPCIALFPYTDPALRDLSGSEALNAKNLVCKSVRAIKREFPDLGVLCDVALDPFTSHGHDGLVEDGRILNDETVAVLVQQALVQAEAGCDIVAPSDMMDGRVGAIRRALDEAGFVDVQIMAYAAKYASAFYGPFRDAIGSAKALTGDKRTYQMDSANSDEALREVELDIAEGADMVLVKPGMPYLDIVRRVKDTFGMPTFAYQVSGEYAMIAAAAGNGWIDRDRAMMESLVAFKRAGADGVLTYFAAEAAEKLKQNR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-05-26" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRE4</accession>
    <name>NUOB_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADH-quinone oxidoreductase subunit B</fullName>
            <ecNumber evidence="1">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">NADH dehydrogenase I subunit B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">NDH-1 subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nuoB</name>
        <name type="ordered locus">Nwi_1887</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">NDH-1 is composed of 14 different subunits. Subunits NuoB, C, D, E, F, and G constitute the peripheral sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 20 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRE4"/>
    <dbReference type="SMR" id="Q3SRE4"/>
    <dbReference type="STRING" id="323098.Nwi_1887"/>
    <dbReference type="EnsemblBacteria" id="ABA05147">
        <property type="protein sequence ID" value="ABA05147"/>
        <property type="gene ID" value="Nwi_1887"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1887"/>
    <dbReference type="eggNOG" id="COG0377">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055737_7_0_5"/>
    <dbReference type="OMA" id="AGWVRKS"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01356">
        <property type="entry name" value="NDH1_NuoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006138">
        <property type="entry name" value="NADH_UQ_OxRdtase_20Kd_su"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01957">
        <property type="entry name" value="nuoB_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01150">
        <property type="entry name" value="COMPLEX1_20K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0874">Quinone</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <keyword id="KW-0830">Ubiquinone</keyword>
    <feature type="chain" id="PRO_0000376289" description="NADH-quinone oxidoreductase subunit B">
        <location>
            <begin position="1"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01356"/>
        </source>
    </evidence>
    <sequence length="207" mass="22593" checksum="28FCEAADDCC8C416" modified="2005-10-11" version="1">MEERRSRMGLNSPTSKVAYAGGTVTGAAGTSILDPATGRPVGASDPYFLEVNHELSDKGFFVAAADDLITWARTGSLMWMTFGLACCAVEMMQVSMPRYDVERFGFAPRASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGILLLQKKIRRVGTIER</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRK5</accession>
    <name>Q3SRK5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Outer membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1826</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315082.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1826"/>
    <dbReference type="EnsemblBacteria" id="ABA05086">
        <property type="protein sequence ID" value="ABA05086"/>
        <property type="gene ID" value="Nwi_1826"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1826"/>
    <dbReference type="eggNOG" id="COG4625">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1426645_0_0_5"/>
    <dbReference type="OrthoDB" id="1713183at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005546">
        <property type="entry name" value="Autotransporte_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036709">
        <property type="entry name" value="Autotransporte_beta_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03797">
        <property type="entry name" value="Autotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103515">
        <property type="entry name" value="SSF103515"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51208">
        <property type="entry name" value="AUTOTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Autotransporter" evidence="2">
        <location>
            <begin position="1"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="190" mass="20234" checksum="7AA3DA4103CC6EDC" modified="2005-10-11" version="1">MAPTGERLRGAYKASVFSGRIEAGWRVETALAGVTPYAAAQAISYRLPSYLEQGNGAIDSFALGYVARNVSAPRSELGLRLDRTTVMADALLTWRGRAAWARNFNTARDVAATFQSLPGTGFLVSGAAMAPDAALVPPEPKSTGATASRLPRTSRASSPTTYPATPARERCDTRGEKLRAGVSPRFESWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRR8</accession>
    <name>Q3SRR8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF3597 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1762</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315019.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1762"/>
    <dbReference type="EnsemblBacteria" id="ABA05023">
        <property type="protein sequence ID" value="ABA05023"/>
        <property type="gene ID" value="Nwi_1762"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1762"/>
    <dbReference type="eggNOG" id="ENOG5032RKY">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114097_1_0_5"/>
    <dbReference type="OMA" id="DWKHSIV"/>
    <dbReference type="OrthoDB" id="2014793at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022016">
        <property type="entry name" value="DUF3597"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12200">
        <property type="entry name" value="DUF3597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3597" evidence="2">
        <location>
            <begin position="3"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="124" mass="12902" checksum="C132B8657A395C45" modified="2005-10-11" version="1">MSIFGKIMGAIFGSHAEAATPAGESGSGTAPSPEGSSAKSVDVAAILDKAVAAKKEKLAWRTSIVDLMKALDVDSSLSARKELAKDLKYPGDTNDSAAMNVWLHKQMMAQLAANGGKLPPDMKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRT4</accession>
    <name>Q3SRT4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1746</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05007">
        <property type="protein sequence ID" value="ABA05007"/>
        <property type="gene ID" value="Nwi_1746"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1746"/>
    <dbReference type="eggNOG" id="ENOG5030K8R">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_181309_0_0_5"/>
    <dbReference type="OMA" id="RRCRHWG"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229157" evidence="1">
        <location>
            <begin position="24"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="87" mass="9651" checksum="B4B0470DF194C542" modified="2005-10-11" version="1">MMKSVFAATFLVATIATFGMADAMPLAPPAAGVNADLIQVAHRCGPGYHRGPRGVCIANRRGPAVDRRQHRRCRHWGPGRRICRTWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRX0</accession>
    <name>Q3SRX0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chitooligosaccharide deacetylase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Nodulation protein B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_1710</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Is involved in generating a small heat-stable compound (Nod), an acylated oligomer of N-acetylglucosamine, that stimulates mitosis in various plant protoplasts.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the polysaccharide deacetylase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314969.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1710"/>
    <dbReference type="EnsemblBacteria" id="ABA04971">
        <property type="protein sequence ID" value="ABA04971"/>
        <property type="gene ID" value="Nwi_1710"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1710"/>
    <dbReference type="eggNOG" id="COG0726">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041251_0_0_5"/>
    <dbReference type="OMA" id="DDWRHIS"/>
    <dbReference type="OrthoDB" id="1701876at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0536">Nodulation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229236" description="Chitooligosaccharide deacetylase" evidence="8">
        <location>
            <begin position="35"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="NodB homology" evidence="9">
        <location>
            <begin position="74"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="373"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA04971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="427" mass="46817" checksum="69DC35793D172EDC" modified="2005-10-11" version="1">MARISKAARRRIQRILGLGLLAATLEFTALPALAGDCPGNPDALGTSRTLVVDPREHPRIGTMQYQETLPLRDHEVVLTFDDGPLPPHSLRVLDILARECVKATFFTVGRMARDYPQHLRRVLAAGHSIGTHSQNHPLGMNRMPIERARQEIDDGIASATAALGDEAALAPFMRIPGLRRAQGIEDYLASRNIQLWSADFPADDWRPISAERVYQLAISRIEAKGKGILLLHDIQARTVTALPRILRTLKERGYRIVHVVPARANRPKTATEPHDWRMHPAPAIVATASPSPAVQATGPVDAGSLPAPAIPDSQLNQGQLVLLPEVLDRSQRFARGFLPLLLRDWAFWRRPSPLAAYDRLSVPRQNFFTIQEKPPGPLAAAPLPQQARPPVGSGHPIEGNEQSMPLDASAAKRTIPGSAPALRSTFR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRZ3</accession>
    <name>ACP_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acyl carrier protein</fullName>
            <shortName evidence="1">ACP</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">acpP</name>
        <name type="ordered locus">Nwi_1687</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Carrier of the growing fatty acid chain in fatty acid biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="1">4'-phosphopantetheine is transferred from CoA to a specific serine of apo-ACP by AcpS. This modification is essential for activity because fatty acids are bound in thioester linkage to the sulfhydryl of the prosthetic group.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the acyl carrier protein (ACP) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_002716125.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRZ3"/>
    <dbReference type="SMR" id="Q3SRZ3"/>
    <dbReference type="STRING" id="323098.Nwi_1687"/>
    <dbReference type="EnsemblBacteria" id="ABA04948">
        <property type="protein sequence ID" value="ABA04948"/>
        <property type="gene ID" value="Nwi_1687"/>
    </dbReference>
    <dbReference type="GeneID" id="66894155"/>
    <dbReference type="KEGG" id="nwi:Nwi_1687"/>
    <dbReference type="eggNOG" id="COG0236">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108696_5_1_5"/>
    <dbReference type="OMA" id="CEIPDEQ"/>
    <dbReference type="OrthoDB" id="1943389at2"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000036">
        <property type="term" value="F:acyl carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031177">
        <property type="term" value="F:phosphopantetheine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01217">
        <property type="entry name" value="Acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003231">
        <property type="entry name" value="Acyl_carrier"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020806">
        <property type="entry name" value="PKS_PP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863">
        <property type="entry name" value="PTHR20863"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00823">
        <property type="entry name" value="PKS_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="SSF47336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00517">
        <property type="entry name" value="acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword id="KW-0276">Fatty acid metabolism</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0596">Phosphopantetheine</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000066645" description="Acyl carrier protein">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="2">
        <location>
            <begin position="2"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="2">
        <location>
            <position position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00258"/>
        </source>
    </evidence>
    <sequence length="79" mass="8573" checksum="058D3A9ADA76B833" modified="2005-10-11" version="1">MSEIGERVKKIVVEHLGVEPEKVVDSASFIDDLGADSLDTVELVMAFEEEFGCEIPDDAAETILTVGDATKFLEKNAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS36</accession>
    <name>Q3SS36_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Lytic murein transglycosylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1644</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314904.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1644"/>
    <dbReference type="CAZy" id="GH103">
        <property type="family name" value="Glycoside Hydrolase Family 103"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04905">
        <property type="protein sequence ID" value="ABA04905"/>
        <property type="gene ID" value="Nwi_1644"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1644"/>
    <dbReference type="eggNOG" id="COG2951">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035402_0_2_5"/>
    <dbReference type="OMA" id="HMRPVFP"/>
    <dbReference type="OrthoDB" id="710313at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.101.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043426">
        <property type="entry name" value="MltB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011970">
        <property type="entry name" value="MltB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036366">
        <property type="entry name" value="PGBDSf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031304">
        <property type="entry name" value="SLT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30163">
        <property type="entry name" value="PTHR30163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13406">
        <property type="entry name" value="SLT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="SSF47090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02283">
        <property type="entry name" value="MltB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229284" evidence="1">
        <location>
            <begin position="33"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="SLT_2" evidence="3">
        <location>
            <begin position="38"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="PG_binding_1" evidence="2">
        <location>
            <begin position="349"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="407" mass="45298" checksum="428E566E54120448" modified="2005-10-11" version="1">MTVPDSHIRLSRRGALRCLGAVAVLAPFPALAAMPQGFEQWRYGFRARALAKGISSATWSRCMGRIEPDMSVFNKMRKQPEFHEKIWQYINRRVSDWRIIAGKEALRKNEALFSRIERDFGVERGTLLALWGVETAFGDPLVQKNHMRPVFPSLAALAWNEPRRRAYWETELINALRIVDKGWSTPEEMRGSWAGAMGHSQWMPEVWLNVGIDYDRDGRVSPFGKPDDALGSTARYLIKRGKYHRGEHWGYEVRAPGGASGSRSYAAWAAAGVQRADGQVFPQPNASAKLWIPEPGGPAFLLGPNFYAVRSYNPSMNYALSICHLGDRILGAPPFIQPFPGSERALTLAEVQEVQTRLTQAGFDTGGTDGRVGNDTMKAVKDFQARVGLPPDGYAGLKVLAKLRQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSK9</accession>
    <name>Q3SSK9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1471</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314739.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1471"/>
    <dbReference type="EnsemblBacteria" id="ABA04732">
        <property type="protein sequence ID" value="ABA04732"/>
        <property type="gene ID" value="Nwi_1471"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1471"/>
    <dbReference type="eggNOG" id="COG1511">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5281">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008236_0_0_5"/>
    <dbReference type="OMA" id="PNHAMAG"/>
    <dbReference type="OrthoDB" id="1006982at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006431">
        <property type="entry name" value="Phage_tape_meas_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013491">
        <property type="entry name" value="Tape_meas_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09718">
        <property type="entry name" value="Tape_meas_lam_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20155">
        <property type="entry name" value="TMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02675">
        <property type="entry name" value="tape_meas_nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TMP_3" evidence="2">
        <location>
            <begin position="91"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Tape_meas_lam_C" evidence="1">
        <location>
            <begin position="484"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="668" mass="69704" checksum="59A36C19CA6DE362" modified="2005-10-11" version="1">MAEDTGERLVVMLEARISEFEKRMVKAEQTGTRTYRQLTAGSSRATKNMEQDMLRSTTAINRSLAQTSVQIGSFAKAFAVGIAGSAAFRTVSKAAQEFTSLQNALKVTGLEDAALDKVFSSLFQIAQKNGTAIEPLVTLYSRASQAQKELKANSADLMKFTDGVSIALRVAGTNSTEAAGALLQLSQAIGSGVVRAEEFNSVNEGARPILQAVAAGLKEAGGSVSTLKNLVNDGKVSSEAFFRAFLAGMPQIEAQAAKADGTIGQASARIGNAFIALVGHLDKTTGASGNAAKGLTAVAGVVEKLSDYFDAASGKLETLQKWLTSVGNNSAWTTIAKFFGADFSPEEMAKYGLTPVGATPATPARVLVTGGTTEGPKVNPVSLADFKVPGKKDGAAATRDPFERSNDQIAKRIELLKVEAATIDQTATEQDRARVVVELETAAKQANERAGKKNVEVTAEQRAKIEALADAYAKARGELERLNSPLASFARESADVGRQLEYLSVGSLDRIADDFGEIVTGTKTVEEAFKSMAQSILAELAKIAVKQAVLGPLANLMGGGGSGGGILSALIPKFANGTDNAPGGLAIVGEKGPELVNLPRGSQVIPNHQIGSVGGGGDINVPVMVNIDATGADAAGLQRVEQQVADLKANLPMLTVQAVRNAKNRRLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSV8</accession>
    <name>RL29_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpmC</name>
        <name type="ordered locus">Nwi_1372</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL29 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314647.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSV8"/>
    <dbReference type="SMR" id="Q3SSV8"/>
    <dbReference type="STRING" id="323098.Nwi_1372"/>
    <dbReference type="PRIDE" id="Q3SSV8"/>
    <dbReference type="EnsemblBacteria" id="ABA04633">
        <property type="protein sequence ID" value="ABA04633"/>
        <property type="gene ID" value="Nwi_1372"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1372"/>
    <dbReference type="eggNOG" id="COG0255">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158491_1_0_5"/>
    <dbReference type="OMA" id="RFQMATS"/>
    <dbReference type="OrthoDB" id="2063644at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00374">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001854">
        <property type="entry name" value="Ribosomal_L29/L35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036049">
        <property type="entry name" value="Ribosomal_L29/L35_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018254">
        <property type="entry name" value="Ribosomal_L29_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00831">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46561">
        <property type="entry name" value="SSF46561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00012">
        <property type="entry name" value="L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00579">
        <property type="entry name" value="RIBOSOMAL_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_1000007542" description="50S ribosomal protein L29">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00374"/>
        </source>
    </evidence>
    <sequence length="68" mass="7868" checksum="4832EA695822AA2B" modified="2005-10-11" version="1">MAAMKTADIRAMTPDQMDDAITSLKKERFNLRFQRATGQLENTSRMREARRDIARIKTIAAQKRDAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSX7</accession>
    <name>Q3SSX7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1353</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1353"/>
    <dbReference type="EnsemblBacteria" id="ABA04614">
        <property type="protein sequence ID" value="ABA04614"/>
        <property type="gene ID" value="Nwi_1353"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1353"/>
    <dbReference type="eggNOG" id="COG0654">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2343858_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="97" mass="10789" checksum="7528A37C748EE42C" modified="2005-10-11" version="1">MMYDTRVNAMRTQIPSSIESFYTKVTEVATSDERQRVVLASGEEISARLIVLANGLSISLRHFLGLGRRVISECHSVTLGFDVEPIDRPVLPFPSLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSZ2</accession>
    <name>Q3SSZ2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">TonB-dependent siderophore receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_1338</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the TonB-dependent receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1338"/>
    <dbReference type="EnsemblBacteria" id="ABA04599">
        <property type="protein sequence ID" value="ABA04599"/>
        <property type="gene ID" value="Nwi_1338"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1338"/>
    <dbReference type="eggNOG" id="COG4773">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008287_9_0_5"/>
    <dbReference type="OMA" id="HYEWANQ"/>
    <dbReference type="OrthoDB" id="173242at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015344">
        <property type="term" value="F:siderophore uptake transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012910">
        <property type="entry name" value="Plug_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037066">
        <property type="entry name" value="Plug_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011662">
        <property type="entry name" value="Secretin/TonB_short_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039423">
        <property type="entry name" value="TonB-dep_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000531">
        <property type="entry name" value="TonB-dep_rcpt_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036942">
        <property type="entry name" value="TonB_rcpt_b-brl_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010105">
        <property type="entry name" value="TonB_sidphr_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32552">
        <property type="entry name" value="PTHR32552"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07715">
        <property type="entry name" value="Plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00593">
        <property type="entry name" value="TonB_dep_Rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00965">
        <property type="entry name" value="STN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01783">
        <property type="entry name" value="TonB-siderophor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="4 8" id="KW-0798">TonB box</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229174" evidence="9">
        <location>
            <begin position="39"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="domain" description="STN" evidence="10">
        <location>
            <begin position="70"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA04599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="815" mass="88061" checksum="744C7DDCEE64CE7D" modified="2005-10-11" version="1">MGDKRMGRMRQSQVKGRTRSLLLATTLLTTGFAVPAAAQNAPASLAARTISIPAGPLTSALNQLAAQTGMQVLFDASIARGKTTRGAQGALTANQALSVILNGTGLVARATGPNAITIVGPGASATGAAPAGAIALDTIDIQGETAWGPVQGYVANRSLTGSKTDTPIIEIPQSLSVIGAEQIRDQKLVSKFDEVLRYTPGVVAGTFGTDHRDDWFLIRGFDAQNDSLFLDGMQLSYFAFGGFKMQPFNLERVEVLRGPSASLYGGSGPGGLVNAVSKLPPAEPLHYIEAGVNNFGNAYTQFDFGGPVATQPGNGKMLYRIVGQVQGGGTQTNFVNNDNYFIAPSVTWLPDIDTRLTVFAMASRTRTRASNFLPYVGTATDAPFGRIPTSLFTGDPSADRYTRTQEMVSYQFEKHLTDNATFRQNARAAHVEVNYTGLYGQGWATTPAAANITRGNFLAASKATQLNMDNQFEYRFATGPFRHTTLMGLNLKHYAIDDRQGIGPGTDLNVLNPVYGVNAPFDGPLYQDGYLTQGQLGIYLQDQIKWNRFTLILSGRNDWVGLVFNNRLGPSPNRNDSKFTGRAGLIYNFDNGLAPYASYSTSYNPIVGLNPFGGLLLPETAEQTEVGMKYQPVGLNARFGIALFDLKRKNALTTDPTNVLFQTQNGEVTSRGIELEALANITRDFKVVASYTNYALFVSKDLDPTLIGKVPTAKPRQVASLWMDYTFRDNWLDGFGFGGGVRYVGSSFADAANTLSVPSVVLGDLAVHYEWANQWRAAINVVNIADTQYVASCGFVTSCFYGDRRRITGSLSYKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="127" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST13</accession>
    <name>Q3ST13_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Glutamate racemase</fullName>
            <ecNumber evidence="2 7">5.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">murI</name>
        <name evidence="8" type="ordered locus">Nwi_1317</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Provides the (R)-glutamate required for cell wall biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>L-glutamate = D-glutamate</text>
            <dbReference type="Rhea" id="RHEA:12813"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29986"/>
            <dbReference type="EC" id="5.1.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the aspartate/glutamate racemases family.</text>
    </comment>
    <dbReference type="EC" id="5.1.1.3" evidence="2 7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314596.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1317"/>
    <dbReference type="EnsemblBacteria" id="ABA04578">
        <property type="protein sequence ID" value="ABA04578"/>
        <property type="gene ID" value="Nwi_1317"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1317"/>
    <dbReference type="eggNOG" id="COG0796">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052344_2_0_5"/>
    <dbReference type="OMA" id="MPWGPRT"/>
    <dbReference type="OrthoDB" id="1718671at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008881">
        <property type="term" value="F:glutamate racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00258">
        <property type="entry name" value="Glu_racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015942">
        <property type="entry name" value="Asp/Glu/hydantoin_racemase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001920">
        <property type="entry name" value="Asp/Glu_race"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018187">
        <property type="entry name" value="Asp/Glu_racemase_AS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033134">
        <property type="entry name" value="Asp/Glu_racemase_AS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004391">
        <property type="entry name" value="Glu_race"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21198:SF2">
        <property type="entry name" value="PTHR21198:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01177">
        <property type="entry name" value="Asp_Glu_race"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53681">
        <property type="entry name" value="SSF53681"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00067">
        <property type="entry name" value="glut_race"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00923">
        <property type="entry name" value="ASP_GLU_RACEMASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00924">
        <property type="entry name" value="ASP_GLU_RACEMASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0133">Cell shape</keyword>
    <keyword evidence="6 7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5 7 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 7" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="11"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="43"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="76"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="193"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="7">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="7">
        <location>
            <position position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA04578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="271" mass="28480" checksum="1C62A519ABBEA4F6" modified="2005-10-11" version="1">MSYSPTILVLDSGLGGLTVLREIVNALPAARYIYVADDAFFPYGRLSEEDIIARVVPLVGELIGAHAPDIVVIACNTMSVSVLGPLRAAYPAPFVGTVPAIKPACAASKTKRVSVLGTRATVQREYTQALIRNHAQACTVTLVGAARLASLAEDALSGKLVSDADILSEITPCFVPAGEGGGLRTDAVVLACTHYPLLLDRLLRLAPWPVSWIDPAPAIARRVVDLLGLLGPLAPNTAGDHDRPPEMIFTSGRSRTLADALAPFFGGRVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST51</accession>
    <name>Q3ST51_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1279</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314568.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04540">
        <property type="protein sequence ID" value="ABA04540"/>
        <property type="gene ID" value="Nwi_1279"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1279"/>
    <dbReference type="HOGENOM" id="CLU_1233963_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="224" mass="25874" checksum="D4C8E13B1E12B687" modified="2005-10-11" version="1">MRKSQFPFREYLPVPGLILLFGAAWFCSQQWLQFRPWWVDVPWIAILATIAAFGLGTFYADLYNDNSQLRRWWADRRRVFNVVFHPASDVKDGYEVQTPYFSVRFIGPVTGHSLELVLYEHLQWSFNKPAQLLLRVDAVKFSEGEHVRVPIATIAQAAHGPHFWGDGTANKSPIPGTGNLVEVRAVKKGRVKQRYSVFIAYRENEAATGNIYSFLQEDRHVFRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST53</accession>
    <name>Q3ST53_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1277</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314566.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04538">
        <property type="protein sequence ID" value="ABA04538"/>
        <property type="gene ID" value="Nwi_1277"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1277"/>
    <dbReference type="HOGENOM" id="CLU_199018_0_0_5"/>
    <dbReference type="OMA" id="FARYPAT"/>
    <dbReference type="OrthoDB" id="2102162at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="76" mass="8470" checksum="C3C6F12C2F9D9636" modified="2005-10-11" version="1">MSLSNVDEFQRYLFEYKFDGAEWGVEIKAASPNEALERLKAISWAKYKGEIYATVPVASARTSRTLARLRAAVGLG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STA8</accession>
    <name>RPIA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribose-5-phosphate isomerase A</fullName>
            <ecNumber evidence="1">5.3.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Phosphoriboisomerase A</fullName>
            <shortName evidence="1">PRI</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpiA</name>
        <name type="ordered locus">Nwi_1221</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible conversion of ribose-5-phosphate to ribulose 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>aldehydo-D-ribose 5-phosphate = D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:14657"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="EC" id="5.3.1.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; pentose phosphate pathway; D-ribose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribose 5-phosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.6" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314512.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3STA8"/>
    <dbReference type="SMR" id="Q3STA8"/>
    <dbReference type="STRING" id="323098.Nwi_1221"/>
    <dbReference type="EnsemblBacteria" id="ABA04483">
        <property type="protein sequence ID" value="ABA04483"/>
        <property type="gene ID" value="Nwi_1221"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1221"/>
    <dbReference type="eggNOG" id="COG0120">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056590_1_0_5"/>
    <dbReference type="OMA" id="YDWDEVN"/>
    <dbReference type="OrthoDB" id="1681738at2"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00412"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004751">
        <property type="term" value="F:ribose-5-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009052">
        <property type="term" value="P:pentose-phosphate shunt, non-oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01398">
        <property type="entry name" value="RPI_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00170">
        <property type="entry name" value="Rib_5P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020672">
        <property type="entry name" value="Ribose5P_isomerase_typA_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004788">
        <property type="entry name" value="Ribose5P_isomerase_type_A"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06026">
        <property type="entry name" value="Rib_5-P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00021">
        <property type="entry name" value="rpiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000016956" description="Ribose-5-phosphate isomerase A">
        <location>
            <begin position="1"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="28"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="83"/>
            <end position="86"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="96"/>
            <end position="99"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00170"/>
        </source>
    </evidence>
    <sequence length="232" mass="24637" checksum="48B37D363A77D8BD" modified="2005-10-11" version="1">MTMDALKRQAAARALEYVEDGMRLGLGTGSTAKHFVELLGERVRDGLKVVGVPTSEATRADAERCGVPLTTLDDLDRLDLTVDGADEIDPDLNLTKGGGGALLREKIVAAASDRMIVIADETKWVAGLGRFPLPIEVIPFGLAATRRAITEVFAKAGVSGELAVRKRPDGHAFVTDGGHWIFDAHLGQIPDASYLAGLLNPIPGVVEHGLFIGLARVAMLAGAEGIRIVERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STB5</accession>
    <name>Q3STB5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glyoxalase/bleomycin resistance protein/dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1214</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314505.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1214"/>
    <dbReference type="EnsemblBacteria" id="ABA04476">
        <property type="protein sequence ID" value="ABA04476"/>
        <property type="gene ID" value="Nwi_1214"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1214"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046006_12_4_5"/>
    <dbReference type="OMA" id="FPGAWLY"/>
    <dbReference type="OrthoDB" id="1984437at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="5"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="129" mass="14342" checksum="4FFED9A57EEC1EF0" modified="2005-10-11" version="1">MSVGMLDHFNIRTRKLAETVRFYEEVLGLTKGDRPDFAFPGAWLYSEGKPVVHLVDIAPTSEPQKPDSGVVHHIAFASRDYRGMKQRLEAKGFAFKAREVPGGHIWQIFVSDPNGVLIELNYDAAAEGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STI8</accession>
    <name>Q3STI8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1141</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314434.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1141"/>
    <dbReference type="EnsemblBacteria" id="ABA04403">
        <property type="protein sequence ID" value="ABA04403"/>
        <property type="gene ID" value="Nwi_1141"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1141"/>
    <dbReference type="HOGENOM" id="CLU_1538487_0_0_5"/>
    <dbReference type="OrthoDB" id="2065725at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="174" mass="19853" checksum="0A66F4B82F66945D" modified="2005-10-11" version="1">MPASLAAGYCGESTVDAFLKRVGKEYPLPRVKGLRGFMTAKVEDLKIKLNDAVEQGKMLLAAKNHWMDRALCAEQERENVFRVVEDTARDLGCEADKEAIVSAIARLKQERDEAYERLKPFAKINPVEITFSKSEKMFRIDYDAAFQDQLAFRHFRAARDIIRQLPSGPEKEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STJ5</accession>
    <name>Q3STJ5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Flagellar hook-basal body complex protein FliE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">fliE</name>
        <name evidence="4" type="ordered locus">Nwi_1134</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 3">Bacterial flagellum basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the FliE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314427.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1134"/>
    <dbReference type="EnsemblBacteria" id="ABA04396">
        <property type="protein sequence ID" value="ABA04396"/>
        <property type="gene ID" value="Nwi_1134"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1134"/>
    <dbReference type="eggNOG" id="COG1677">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147249_2_2_5"/>
    <dbReference type="OMA" id="SAYEDIM"/>
    <dbReference type="OrthoDB" id="1581972at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00724">
        <property type="entry name" value="FliE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001624">
        <property type="entry name" value="FliE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34653">
        <property type="entry name" value="PTHR34653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02049">
        <property type="entry name" value="FliE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="4" id="KW-0282">Flagellum</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="103" mass="10345" checksum="8A346E33736CDDE8" modified="2005-10-11" version="1">MASPTVAANAYASLARMMDSGAVAGKAPGGDGPSFGAVLKDVLGGVVEAGRASDAQTVAMASGKANLMDVVTAVADTDVAVSTLVSVRDRVIQSYEDIMRMPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STL7</accession>
    <name>Q3STL7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable flagellar assembly protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1112</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314405.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1112"/>
    <dbReference type="EnsemblBacteria" id="ABA04374">
        <property type="protein sequence ID" value="ABA04374"/>
        <property type="gene ID" value="Nwi_1112"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1112"/>
    <dbReference type="eggNOG" id="ENOG50330GZ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139719_0_0_5"/>
    <dbReference type="OMA" id="RARRENC"/>
    <dbReference type="OrthoDB" id="1788889at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044781">
        <property type="term" value="P:bacterial-type flagellum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019704">
        <property type="entry name" value="Flagellar_assmbl_FliX_class2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10768">
        <property type="entry name" value="FliX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0282">Flagellum</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="137" mass="14291" checksum="2B7EE55F184BFE3D" modified="2005-10-11" version="1">MRIYGANGTTPGTQAPSARRTGGSEFSLPDAASATESRAAAAPKATATIDALLALQGVEEDPVERRRRSVRRGRGALDVLDDLKIGLLSGNFDPATVTRLRTAANELKSSSGDAGLDAVLAEIELRVEVELAKAGQF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STL8</accession>
    <name>FLGI_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Flagellar P-ring protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Basal body P-ring protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">flgI</name>
        <name type="ordered locus">Nwi_1111</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Assembles around the rod to form the L-ring and probably protects the motor/basal body from shearing forces during rotation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The basal body constitutes a major portion of the flagellar organelle and consists of four rings (L,P,S, and M) mounted on a central rod.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Bacterial flagellum basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FlgI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314404.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3STL8"/>
    <dbReference type="SMR" id="Q3STL8"/>
    <dbReference type="STRING" id="323098.Nwi_1111"/>
    <dbReference type="EnsemblBacteria" id="ABA04373">
        <property type="protein sequence ID" value="ABA04373"/>
        <property type="gene ID" value="Nwi_1111"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1111"/>
    <dbReference type="eggNOG" id="COG1706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045235_1_0_5"/>
    <dbReference type="OMA" id="VGPRDMI"/>
    <dbReference type="OrthoDB" id="693640at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009428">
        <property type="term" value="C:bacterial-type flagellum basal body, distal rod, P ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030288">
        <property type="term" value="C:outer membrane-bounded periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00416">
        <property type="entry name" value="FlgI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001782">
        <property type="entry name" value="Flag_FlgI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30381">
        <property type="entry name" value="PTHR30381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02119">
        <property type="entry name" value="FlgI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01010">
        <property type="entry name" value="FLGPRINGFLGI"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0975">Bacterial flagellum</keyword>
    <keyword id="KW-0574">Periplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000236305" description="Flagellar P-ring protein">
        <location>
            <begin position="30"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="296"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="374" mass="38693" checksum="1E54C05A6789FE10" modified="2005-10-11" version="1" precursor="true">MSGLGFTGVVRIAVMALLALAFLGAPAHATSRIKDLANIEGVRQNQLIGYGLVVGLNGTGDTLNNIPFTKQSLQAMLERMGVNIRGATIRTGNVAAVMVTGNLPAFATQGTRMDVTVSAMGDARSLQGGTLLVTPLLGADGNVYAVAQGSLAIGGFSAEGAAASVTKGVPTNGRIANGAIVEREIEFALNRMPNVRLALRNGDFTTAKRIAAAVNDFLGTKTAEPIDPSTVQLSIPAEFRGNVVALLTEIEQLQVEPDITAKIIIDERSGIIVMGRDVRVATVAVAQGNLTVSISESPQVSQPNPLSNGRTVMTPRTDVGVTENGNKLALVKNGVSLQELVDGLNGLGIGPRDLIGILQAIKAAGAIEADIEVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STM2</accession>
    <name>Q3STM2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1107</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314400.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04369">
        <property type="protein sequence ID" value="ABA04369"/>
        <property type="gene ID" value="Nwi_1107"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1107"/>
    <dbReference type="eggNOG" id="ENOG5030VWX">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2045194_0_0_5"/>
    <dbReference type="OMA" id="YQSEGTI"/>
    <dbReference type="OrthoDB" id="1733915at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="121" mass="13254" checksum="BBFD32B510141873" modified="2005-10-11" version="1">MADVLNILASNYRTVGENVSLTTGKYVNVDPDVFEGTWSGKYANQKPFSITISNVNGFRAKAKYQYGTTIKYQEVLIKNNGFRVGDSKFVLTKTGTAQVSTVMSDPATGASTLETAYVHQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STM5</accession>
    <name>Q3STM5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Flagellin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1104</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Bacterial flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial flagellin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314397.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1104"/>
    <dbReference type="EnsemblBacteria" id="ABA04366">
        <property type="protein sequence ID" value="ABA04366"/>
        <property type="gene ID" value="Nwi_1104"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1104"/>
    <dbReference type="eggNOG" id="COG1344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013955_1_0_5"/>
    <dbReference type="OMA" id="YSIANTM"/>
    <dbReference type="OrthoDB" id="636786at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009288">
        <property type="term" value="C:bacterial-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046358">
        <property type="entry name" value="Flagellin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001029">
        <property type="entry name" value="Flagellin_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00700">
        <property type="entry name" value="Flagellin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00669">
        <property type="entry name" value="Flagellin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Flagellin_N" evidence="2">
        <location>
            <begin position="12"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Flagellin_C" evidence="3">
        <location>
            <begin position="438"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU362073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="523" mass="52406" checksum="A9AA198C676B2B0F" modified="2005-10-11" version="1">MSDIVLSASVRQNLLSLQSTAELLSTTQTRLATGKKVNSALDNPTNFFTAAGLDARASDINNLLDSIGNGVQVLQAANTGITSLQKLVDTAKSIANQALQATSGYSTKSQATTAAITGATADNLLGPGGAPTDAAATPGSVVNNSLTVPAPITAATTLSGGVNTDSLATDFIAGDTIVVNGTTINFVASGATGNDLNITDTVGDVLARIDSITGGTSTISGGAITLHTGTASDLTISGSGLAKLGLTAGTTARTPGAPAALDGKTLTIGATDGGTATSITFGTGTGQINTLDQLNTALAANNLTASINSAGAITFTTTNDHASATIGAFGGDATTDPNTFGAMTASAPVEDANAQNTRATLIGQYNQVIDQIRTTAQDASFNGINLLNGDQLKLVFNETGKSTLTIQGVTFDPAGLGLSSLAKGTDFLDNASTNAVLDSLNTASSTLRSQASTFGSNLSIVQIRQDFSKNLINVLQTGSANLTLADTNEEAANSQALATRQSIAVSALALANQSQQSVLQLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STM7</accession>
    <name>Q3STM7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Pyridoxal-dependent decarboxylase</fullName>
            <ecNumber evidence="7">4.1.1.28</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_1102</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5 6">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the group II decarboxylase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.28" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314395.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1102"/>
    <dbReference type="EnsemblBacteria" id="ABA04364">
        <property type="protein sequence ID" value="ABA04364"/>
        <property type="gene ID" value="Nwi_1102"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1102"/>
    <dbReference type="eggNOG" id="COG0076">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011856_0_4_5"/>
    <dbReference type="OMA" id="NPGFNWS"/>
    <dbReference type="OrthoDB" id="1478871at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004058">
        <property type="term" value="F:aromatic-L-amino-acid decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:cellular amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010977">
        <property type="entry name" value="Aromatic_deC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021115">
        <property type="entry name" value="Pyridoxal-P_BS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00800">
        <property type="entry name" value="YHDCRBOXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00392">
        <property type="entry name" value="DDC_GAD_HDC_YDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="4 6 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="5">
        <location>
            <position position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="492" mass="53659" checksum="09606D3736559ED3" modified="2005-10-11" version="1">MKPASRGTLPEADETLDPEDWDTLRAEGHRMLDDMFDHIAGVRERPVWRPIPAETRARFREDLPRNGVDVAEIYREFAEHIAPYTAGNIHPGFMGWVNGGGTAVGMLAEMLAAGLNTNVAGRNQMPVEVERQIVLWVRRMFGFPDTASGVFVTGTSMANLMGILVARSARIGPSVRQRGLLDRGARLIAYTSVATHGCIAQAMDCAGFGADALRRIPVDRCHRIDTDALRAQIAADRAAGFEPFLVIGSAGTVDIGAIDDLAVLHEVCRDEKLWLHIDGAFGALGVLSPEIAPRLAGIESADSIAFDFHKWGQVPYDAGFLLVRDGEQHRRTFAAPAAYLRRETRGLAAGSPWPCDYSLDMSRGFRALKTWFTLKAYGTERLGAMIDRTCALARCLEAKVAAEPKLELLAPVQLNIVCFRYRADDADSVNAEIVASLHESGITAPSTTMLDGRLAIRAAIVNHRTGMRDIEALISAVLELGARKSRGSMIFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STQ3</accession>
    <name>Q3STQ3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oxidoreductase</fullName>
            <ecNumber evidence="2">1.1.1.18</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1076</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.18" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314372.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1076"/>
    <dbReference type="EnsemblBacteria" id="ABA04338">
        <property type="protein sequence ID" value="ABA04338"/>
        <property type="gene ID" value="Nwi_1076"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1076"/>
    <dbReference type="eggNOG" id="COG0673">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023194_10_0_5"/>
    <dbReference type="OMA" id="GWHHARV"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050112">
        <property type="term" value="F:inositol 2-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GFO_IDH_MocA" evidence="1">
        <location>
            <begin position="27"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="345" mass="36510" checksum="680C67DBD0474EB4" modified="2005-10-11" version="1">MSSKASALKTAALKPSALKTAASERVLRVGVIGAGVMGSNHGRVLAGLPGVRLIGIVDPLPGHRARAAEMIGCRTFGTFDELLDAGVDAVTIAAPTHLHCELALACIARGVHVLVEKPIASTVDEGYQIVTAARRAGLTLMVGHVERFNPAVAAIKQAIRGEDILSIAITRVGPFPPRMSNVGVVIDLAVHDIDLIRWFTESEIIDVQPQLSCAVAEREDIALLQFRTASGVLAHINTNWLTPFKARNVTVATRGKYVMGDLLTRQVTECFGFQPDGSYSMRHLPVGHDEPLRAELIAFVDAVRSGKAPAVGGAEGVASLEIAIKCLQDEPPVAPPVETARRAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STR2</accession>
    <name>Q3STR2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">D,D-heptose 1,7-bisphosphate phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_1067</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GmhB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314363.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1067"/>
    <dbReference type="EnsemblBacteria" id="ABA04329">
        <property type="protein sequence ID" value="ABA04329"/>
        <property type="gene ID" value="Nwi_1067"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1067"/>
    <dbReference type="eggNOG" id="COG0241">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085077_3_2_5"/>
    <dbReference type="OMA" id="DHPMRKP"/>
    <dbReference type="OrthoDB" id="1472357at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006549">
        <property type="entry name" value="HAD-SF_hydro_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004446">
        <property type="entry name" value="Heptose_bisP_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006543">
        <property type="entry name" value="Histidinol-phos"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42891">
        <property type="entry name" value="PTHR42891"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01662">
        <property type="entry name" value="HAD-SF-IIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01656">
        <property type="entry name" value="Histidinol-ppas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA04329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="184" mass="20567" checksum="E22EC3EF45537D00" modified="2005-10-11" version="1">MSRGDIVPASGPAAFLDRDGVINHDDCYMGTRDRIRWMPGVVPAIRRLNEAGYFVFIFTNQSGVARGFFSEDDVRDLHRWMQSHLASQDARIDDFRYCPHHPDGSVAGYLEDHPWRKPKPGMILDLMRHWPVRCQGSFAIGDRDSDIRAAAGAGIPGFLFPGGDLDAFVARVLAEISDVITPRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STR5</accession>
    <name>Q3STR5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="13">ABC transporter, transmembrane region</fullName>
            <ecNumber evidence="13">3.6.3.27</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Nwi_1064</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in beta-(1--&gt;2)glucan export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.27" evidence="13"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314360.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1064"/>
    <dbReference type="EnsemblBacteria" id="ABA04326">
        <property type="protein sequence ID" value="ABA04326"/>
        <property type="gene ID" value="Nwi_1064"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1064"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_3_5"/>
    <dbReference type="OMA" id="FNTLQMG"/>
    <dbReference type="OrthoDB" id="643917at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="3 10 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="12">
        <location>
            <begin position="35"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="351"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="384"/>
            <end position="391"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA04326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="602" mass="65529" checksum="9054F42EC60D1418" modified="2005-10-11" version="1">MTEPPKKITDDPYGAAILIRRLVTEQGMAYWRRYLAAFALMAVSAAATATSAYLLGEVINQAYVDRNVRGIFMLSTVVLAIFVAKGAATYGHSVILSQISNAILANNQRRLFAKLMSESIGYFSQRHSSEFLARLTAGATSVTQVLSLLINAVGRDLLSLIALVTVMVMQDPYMSLFGALVAPPAMIVLRKLVKRIKGLAHSQFHGTADIMETMQESLQGIRTVKAFTLEQTMRERIDASITAVEQTNNKMARVSNRASPLMETLGGFAIAGGLMYGGYRVVAMGATPGQFFSFLTAFLLAYEPAKRLARLNIELNSSLIGARKLLEIVDSPASEPDDSDKPALQLTDARVELRDVTFAYRPDEPVLKRMSFIAEPGRMTALVGPSGGGKSTVLALLLRLYEVTDGDILIDGQSISGVSRTSLRRQMSYVGQDVYLFRATIRENIAFGRPGATEDEIVAAAKAACAHDFITGFPLGYDTPVGEHGTQLSGGQRQRIAVARALIKDAPIILLDEATAALDSESEKQVQEAIEHLCRNRTTIVIAHRLHTIMHADAILVVENGEIVERGRHDDLLRSGGRYASFFRLQHRETAPLTLAPLSASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STV2</accession>
    <name>Q3STV2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Response regulator receiver</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1027</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314323.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1027"/>
    <dbReference type="EnsemblBacteria" id="ABA04289">
        <property type="protein sequence ID" value="ABA04289"/>
        <property type="gene ID" value="Nwi_1027"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1027"/>
    <dbReference type="eggNOG" id="COG4566">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_8_5"/>
    <dbReference type="OMA" id="VICMSAH"/>
    <dbReference type="OrthoDB" id="1630092at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="14"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="135" mass="14771" checksum="841DBD9665C71D2A" modified="2005-10-11" version="1">MIDTIAKNTVVNHTVGVLDDDPAVLQSLRFLLETHGFKVRTFSSALTLLTSIDPDNVDCLVIDYKLPDIDGLNVAERLRERNVAVPIVLVTGHPHQHMAKKAAAAGIDHILLKPHLGDNLVTHVQEAISRKCADN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STW9</accession>
    <name>Q3STW9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Coenzyme A transferase</fullName>
            <ecNumber evidence="1">2.8.3.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.8.3.5" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1010"/>
    <dbReference type="EnsemblBacteria" id="ABA04272">
        <property type="protein sequence ID" value="ABA04272"/>
        <property type="gene ID" value="Nwi_1010"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1010"/>
    <dbReference type="eggNOG" id="COG1788">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019942_2_0_5"/>
    <dbReference type="OMA" id="FVKAWKG"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008260">
        <property type="term" value="F:3-oxoacid CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012792">
        <property type="entry name" value="3-oxoacid_CoA-transf_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004165">
        <property type="entry name" value="CoA_trans_fam_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13707">
        <property type="entry name" value="PTHR13707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01144">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00882">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02429">
        <property type="entry name" value="pcaI_scoA_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="329" mass="35394" checksum="6D0B0420A7DF4DF0" modified="2005-10-11" version="1">MTPAGSALSGGARNLIGFLDAFKAADRSILTFAGRHGAFDLRSQIAAPRPAIVKFASPGSDKPPIDAPRVFCLTTSRSARAGKRRHSPCRSQLMKKVYPDAKSALDGLLRDGMMIMSGGFGLCGIPETLSEAIRDSGVKDLTFVSNNAGVDGIGLSRLLETRQIRKMISSYVGENKLFAQQYLAGDLELEFNPQGTLAERIRAGGAGIPAFYTKTGVGTLIAEGKEVKEFDGQKYLMERGLFADLAIVHAWKGDTAGNLVYRKTARNFNPMMATAAKVTVAEVEHLVPAGEIDPDHVHTPGIFVKRIVEVGGARKHIEFRNTRPRPAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU11</accession>
    <name>Q3SU11_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Tyrosinase</fullName>
            <ecNumber evidence="3">1.10.3.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0968</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.10.3.1" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314271.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0968"/>
    <dbReference type="EnsemblBacteria" id="ABA04230">
        <property type="protein sequence ID" value="ABA04230"/>
        <property type="gene ID" value="Nwi_0968"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0968"/>
    <dbReference type="eggNOG" id="ENOG502Z8GH">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_448244_0_0_5"/>
    <dbReference type="OMA" id="MHHGNID"/>
    <dbReference type="OrthoDB" id="446922at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004097">
        <property type="term" value="F:catechol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008922">
        <property type="entry name" value="Di-copper_centre_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002227">
        <property type="entry name" value="Tyrosinase_Cu-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00264">
        <property type="entry name" value="Tyrosinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00092">
        <property type="entry name" value="TYROSINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48056">
        <property type="entry name" value="SSF48056"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00497">
        <property type="entry name" value="TYROSINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00498">
        <property type="entry name" value="TYROSINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosinase_Cu-bd" evidence="1">
        <location>
            <begin position="92"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosinase_Cu-bd" evidence="2">
        <location>
            <begin position="240"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="514" mass="55310" checksum="9542D213091B0DFB" modified="2005-10-11" version="1">MTTTRRTVLLQGGAIGAGLIAANVSAVRALAATKTTPVRRSLQGLAWNDPIVATYRDAVGIMKQKPANDKFNWVQLANFHGNISTGFRYCPHGDWYFLPWHRAYTAMYERIVRSLTKNQDFAMPYWDWTASPTMPEVFLPEKTPDGKTNWLCVNEDGRKRTWPPTQPMPANIVGPAVLSTILNASPYEVFGTSRPAGQNSLDPSWITGGGGVQGTLEATPHNQVHNNIGGWMPTAASPRDPIFFMHHGNIDRIWALWNLKHQNSTDPLWTDMPFKENFLNPDGSFWSPKVSDLYVPEKLGYSYGFTQTPVVASSARTMTLERSLNAVLGAKAASKVEAGKVETAVVENNKSATPGAALALKIEVPQAALQGVVRSAPVGSGFDTMDFAAAREASARGSRALAIIRNVAITDPSTTTMQIYLGDEPVPADASPSDRRYVGSFAMLDHGGGGHGAHGDHERPSFVLDLTDALQKVYGSAGTIPGAIGLQLVVSNETGSGKAGSATPQRVEVVLVSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU37</accession>
    <name>Q3SU37_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0942</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0942"/>
    <dbReference type="EnsemblBacteria" id="ABA04204">
        <property type="protein sequence ID" value="ABA04204"/>
        <property type="gene ID" value="Nwi_0942"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0942"/>
    <dbReference type="eggNOG" id="ENOG503306E">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114847_1_0_5"/>
    <dbReference type="OMA" id="CTGIRKM"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229249" evidence="1">
        <location>
            <begin position="28"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="177" mass="18434" checksum="147CC429C432C817" modified="2005-10-11" version="1">MRGKKMSWGPVLGALAVAIVAVGSSEAQEAAQPACTAPVAPTGELAPWTAPSPLQAAGSEAGISSLPVGRAVRLTLLQTPEVRYPLRPEKPGGSVSHGGLVRIDVREAGTYRVALDSAAWIDVVRDKQALRSTAHGHGPNCTGIRKMVDYSLTPGPYILQISANGQPQMTVLLARVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU42</accession>
    <name>Q3SU42_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative pigment protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0937</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314240.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0937"/>
    <dbReference type="EnsemblBacteria" id="ABA04199">
        <property type="protein sequence ID" value="ABA04199"/>
        <property type="gene ID" value="Nwi_0937"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0937"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_2_0_5"/>
    <dbReference type="OMA" id="RYWRDVH"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013107">
        <property type="entry name" value="Acyl-CoA_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08028">
        <property type="entry name" value="Acyl-CoA_dh_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="2">
        <location>
            <begin position="28"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_2" evidence="3">
        <location>
            <begin position="256"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="409" mass="43892" checksum="34B94A13EE59D7D1" modified="2005-10-11" version="1">MAGNDPGAPANPAESDAGDHAYAAVMARARALVPELRDRAARSEELRRLPPETEKEFHDAGLFKILQPRRVGGLELDYVALVDLAEAIAVADASAAWTLANLASHHWMLGMFDRRAQDAVWAKDPNALIASSFVFPSGRATKVEGGYRLQGRWPFSSGVDCCTWNMLAGHVSSDDEADGIEYRIFLVPRERYRIIDMWNAAGLKGTGSNDVAADDLFVEEAMTLSVADIAGGPSPGSAVNPNPLYALPVFALFPFVLSGVALGNAQGCLNDYIEIARHRATTFNRARLGDLQSTQIKIAEASAKIDAARLVMRSICIRAISDAKRGLVPDMAVKTGWRRDGAYAVTLCTEAVSLLFASSGARGLFAAAPLQRQFRDAVAINSHIAFSFDAAGTDYGRVALGLPSQNLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU63</accession>
    <name>Q3SU63_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Periplasmic solute binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0916</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the bacterial solute-binding protein 9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0916"/>
    <dbReference type="EnsemblBacteria" id="ABA04178">
        <property type="protein sequence ID" value="ABA04178"/>
        <property type="gene ID" value="Nwi_0916"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0916"/>
    <dbReference type="eggNOG" id="COG0803">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016838_1_1_5"/>
    <dbReference type="OMA" id="DPHIWFD"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006129">
        <property type="entry name" value="AdhesinB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006128">
        <property type="entry name" value="Lipoprotein_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006127">
        <property type="entry name" value="ZnuA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01297">
        <property type="entry name" value="ZnuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00691">
        <property type="entry name" value="ADHESINB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00690">
        <property type="entry name" value="ADHESNFAMILY"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <keyword evidence="1 3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229210" evidence="4">
        <location>
            <begin position="23"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="307" mass="32122" checksum="FC8AFBD7E8D38B3F" modified="2005-10-11" version="1">MRYGSVIWVIAILLAALAPASAQETAEDRADERIVVVASFSILGDFAQAVGGARTDVTTLVGTDGDAHVFTPTPADARKVAAARLVIVNGLGLEGWLPRLVQASGGHAAIVVAATGVPLRGAEAGQTLRGEDSADPHAWQSVANAKIYVANIRDALIAADPAGEAVYRGNAADYLAKLDALDREVREGVARIPPERRKVISTHDAFGYFEAAYGIAFIAPQGVSTDSEASARDIAATITQIRTAKIPAVFLEDLGDPRLIRQIATEAGARVGGTLYSDSLSDEKGDAPTYIAMVRHNIKALTSALAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUB9</accession>
    <name>Q3SUB9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminopyrimidine aminohydrolase</fullName>
            <ecNumber evidence="1">3.5.99.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0858</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes an amino-pyrimidine hydrolysis reaction at the C5' of the pyrimidine moiety of thiamine compounds, a reaction that is part of a thiamine salvage pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4-amino-5-aminomethyl-2-methylpyrimidine + H2O = 4-amino-5-hydroxymethyl-2-methylpyrimidine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:31799"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16892"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:63416"/>
            <dbReference type="EC" id="3.5.99.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + thiamine = 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17509"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16892"/>
            <dbReference type="ChEBI" id="CHEBI:17957"/>
            <dbReference type="ChEBI" id="CHEBI:18385"/>
            <dbReference type="EC" id="3.5.99.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TenA family.</text>
    </comment>
    <dbReference type="EC" id="3.5.99.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314167.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0858"/>
    <dbReference type="EnsemblBacteria" id="ABA04122">
        <property type="protein sequence ID" value="ABA04122"/>
        <property type="gene ID" value="Nwi_0858"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0858"/>
    <dbReference type="eggNOG" id="COG0819">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077537_1_0_5"/>
    <dbReference type="OMA" id="FYIIQDY"/>
    <dbReference type="OrthoDB" id="1537025at2"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050334">
        <property type="term" value="F:thiaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.910.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016084">
        <property type="entry name" value="Haem_Oase-like_multi-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004305">
        <property type="entry name" value="Thiaminase-2/PQQC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027574">
        <property type="entry name" value="Thiaminase_II"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03070">
        <property type="entry name" value="TENA_THI-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48613">
        <property type="entry name" value="SSF48613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04306">
        <property type="entry name" value="salvage_TenA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0784">Thiamine biosynthesis</keyword>
    <feature type="domain" description="TENA_THI-4" evidence="2">
        <location>
            <begin position="15"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="220" mass="24575" checksum="C98FD9FB15690C62" modified="2005-10-11" version="1">MDVFDRLKAATPADWRSYIDHDFVRQMGAGTLPQTAFRTYLVQDYLFLIQFARVYALATYKSRTLADMKAAQAGLAAIFDEMDLHVRLCDRWGLSPEDIEAAPEHQATIAYTRFVLDCGAAGDLLDLHVALAPCVIGYAEIGRNLAPNGIDALGDHPYREWIAEYAGDAYQAVAANARHHLDDLAARAMTERRFDELATLFGKASRLEADFWQMGLDANR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUI1</accession>
    <name>Q3SUI1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0795</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314108.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0795"/>
    <dbReference type="EnsemblBacteria" id="ABA04060">
        <property type="protein sequence ID" value="ABA04060"/>
        <property type="gene ID" value="Nwi_0795"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0795"/>
    <dbReference type="eggNOG" id="COG5449">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080134_0_0_5"/>
    <dbReference type="OMA" id="WLVNWAD"/>
    <dbReference type="OrthoDB" id="756610at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011928">
        <property type="entry name" value="Phage_phiJL001_Gp84"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018964">
        <property type="entry name" value="Phage_phiJL001_Gp84_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019228">
        <property type="entry name" value="Phage_phiJL001_Gp84_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09931">
        <property type="entry name" value="DUF2163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09356">
        <property type="entry name" value="Phage_BR0599"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02218">
        <property type="entry name" value="phg_TIGR02218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2163" evidence="2">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Phage_BR0599" evidence="1">
        <location>
            <begin position="196"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="295" mass="30774" checksum="9528743EC3D74514" modified="2005-10-11" version="1">MKTLPPALANHVAGGLTTLCRCWRVDRRDGVVMGFTDFDRDLVFDAVTFRAASGFTATAIEGQLGLAVSNLDVQGALSSDALTEDDLHGGRYDDAAVTIYLVNWADVAQRVILRAGNLGQVSRGKLAFSAELRGLAAKLDQPAGRIFQRSCAWDLGDARCGIDLGASGNHGAGAVTDVLDAFEFVASGMSGITSGALTRGRLVWTSGANDGLAVEIKAHSSSAGVSRLAIYLPMGAPVTIGDTFDATAGCDRTFATCRDRFANTVNFGGFPHMPGTDFAMSYPNQGAGNDGGKIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUL1</accession>
    <name>Q3SUL1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Cytochrome c oxidase assembly protein CtaG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ctaG</name>
        <name evidence="12" type="ordered locus">Nwi_0227</name>
        <name evidence="13" type="ordered locus">Nwi_0765</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="13">Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Exerts its effect at some terminal stage of cytochrome c oxidase synthesis, probably by being involved in the insertion of the copper B into subunit I.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cell inner membrane</location>
            <topology evidence="2 10">Single-pass type II membrane protein</topology>
            <orientation evidence="2 10">Periplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the COX11/CtaG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313562.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0227"/>
    <dbReference type="EnsemblBacteria" id="ABA03495">
        <property type="protein sequence ID" value="ABA03495"/>
        <property type="gene ID" value="Nwi_0227"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04030">
        <property type="protein sequence ID" value="ABA04030"/>
        <property type="gene ID" value="Nwi_0765"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0227"/>
    <dbReference type="KEGG" id="nwi:Nwi_0765"/>
    <dbReference type="eggNOG" id="COG3175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045000_5_0_5"/>
    <dbReference type="OMA" id="NKLECFC"/>
    <dbReference type="OrthoDB" id="1821272at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008535">
        <property type="term" value="P:respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00155">
        <property type="entry name" value="CtaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023471">
        <property type="entry name" value="CtaG/Cox11_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007533">
        <property type="entry name" value="Cyt_c_oxidase_assmbl_CtaG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04442">
        <property type="entry name" value="CtaG_Cox11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005413">
        <property type="entry name" value="COX11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110111">
        <property type="entry name" value="SSF110111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-0186">Copper</keyword>
    <keyword evidence="9 10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="topological domain" description="Cytoplasmic" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="topological domain" description="Periplasmic" evidence="10">
        <location>
            <begin position="47"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA03495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA04030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="213" mass="22654" checksum="2589E51B4ECD693E" modified="2005-10-11" version="1">MTDASQHQQSAAPALPKAAPRIGRDVKVGAACGLVVALMLGAAYASVPFYDWFCRVTGFNGTTQVAGSAPQAEPLARTVTVRFDSNVSGGLPWKFEAEQTAIEVRVGEVTTVHYAITNESASPTTGQAAYNVTPLAVGSYFTKINCFCFTEQTLAPGEKREMAVVFYVDQAFAADKEYDGVNTITLSYTFFPVKNAAPRPVAANEPDRPGGSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUN3</accession>
    <name>Q3SUN3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0742</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314062.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04008">
        <property type="protein sequence ID" value="ABA04008"/>
        <property type="gene ID" value="Nwi_0742"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0742"/>
    <dbReference type="eggNOG" id="COG2346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104957_4_1_5"/>
    <dbReference type="OMA" id="RIAWAMH"/>
    <dbReference type="OrthoDB" id="1697277at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="SSF46458"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="140" mass="16260" checksum="8AEF6D948568A2A8" modified="2005-10-11" version="1">MTLVSDKMDDVTEDGIRRLVDAFYTKVRADAELGPVFENKIKDNWPHHLEKMYAFWSSVMLTTGRYKGNPMMKHMAIPGMRPDLFTQWLALFDETCRELCDEPIRAAFMEKAERIAESLKIAVFYRPDRPWPPQASTNAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUU5</accession>
    <name>Q3SUU5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC-type nitrate/sulfonate/bicarbonate transport systems periplasmic components</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0680</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial solute-binding protein SsuA/TauA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0680"/>
    <dbReference type="EnsemblBacteria" id="ABA03946">
        <property type="protein sequence ID" value="ABA03946"/>
        <property type="gene ID" value="Nwi_0680"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0680"/>
    <dbReference type="eggNOG" id="COG0715">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028871_11_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010067">
        <property type="entry name" value="ABC_SsuA_sub-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015168">
        <property type="entry name" value="SsuA/THI5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09084">
        <property type="entry name" value="NMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01728">
        <property type="entry name" value="SsuA_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="PBPb" evidence="4">
        <location>
            <begin position="31"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="239" mass="25843" checksum="F2AB63A57C40D2BA" modified="2005-10-11" version="1">MRSLERLMMAAILSISVFAVSMGVSYAHDKVVRVGYQKYGNLILLKGKGTLEQKLAPLGFKVEWKEFPSGPPLLEALNVDAIDFGHAGESPPIFGQAAGAQFLYVGHEPPAPKGEAILVPKTSPITQVADLKGKKVAFNKGSNVHYLLVKALEKASLKYADIEPVFLAPADARAAFERGSVDAWVMGSVSGFRGSRDGRPHPCRRDGACAQSPVLSRQPEIHLSLPADCRCGDRCARRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUU8</accession>
    <name>Q3SUU8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">4Fe-4S ferredoxin, iron-sulfur binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0677</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313999.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0677"/>
    <dbReference type="EnsemblBacteria" id="ABA03943">
        <property type="protein sequence ID" value="ABA03943"/>
        <property type="gene ID" value="Nwi_0677"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0677"/>
    <dbReference type="eggNOG" id="COG1146">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_5_5_5"/>
    <dbReference type="OMA" id="DMCPLDS"/>
    <dbReference type="OrthoDB" id="1873445at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="10"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="44"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="77" mass="8457" checksum="6B19B66768B565C2" modified="2005-10-11" version="1">MPIAHTPTTVPVVVDEAKCIADKGCTVCVDVCPLDVLRISDLTGKAYMKYDECWYCMPCETDCPTGAVTVNIPYLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUX5</accession>
    <name>Q3SUX5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltranfer_dom domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0650</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313976.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0650"/>
    <dbReference type="EnsemblBacteria" id="ABA03916">
        <property type="protein sequence ID" value="ABA03916"/>
        <property type="gene ID" value="Nwi_0650"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0650"/>
    <dbReference type="eggNOG" id="COG2227">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1022444_0_0_5"/>
    <dbReference type="OMA" id="DRCLINI"/>
    <dbReference type="OrthoDB" id="1664438at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltranfer_dom" evidence="1">
        <location>
            <begin position="52"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="272" mass="29867" checksum="647419F3C48A38E2" modified="2005-10-11" version="1">MDPEQVRAHWRDWASKFGTAIGATTRTRTAKMLEIDALARRMSMFGLGDAPAHVLEVGCGNGLNCFGLAAQFPRMQLDGIDYTAEMIDAAREAAVASGFQDRSRFLIGNALELSAAAGLLAQYDVVFTDRCLINIESVELQMRAITSLAARVRRGGHLIMIENSTATYDRQNHCRTLLNLPARTPAAFNLFFDETRILPHLETLGLEVEIEDFISLHDLMLYVLVPAINGGAVDYDHPLVHAATQLTLAGSPELSRAFGAFGQGRMFVCRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUY2</accession>
    <name>Q3SUY2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DegT/DnrJ/EryC1/StrS aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0642</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the DegT/DnrJ/EryC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313969.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0642"/>
    <dbReference type="EnsemblBacteria" id="ABA03909">
        <property type="protein sequence ID" value="ABA03909"/>
        <property type="gene ID" value="Nwi_0642"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0642"/>
    <dbReference type="eggNOG" id="COG0399">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033332_0_3_5"/>
    <dbReference type="OMA" id="QVHYKPI"/>
    <dbReference type="OrthoDB" id="1722208at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00616">
        <property type="entry name" value="AHBA_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000653">
        <property type="entry name" value="DegT/StrS_aminotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020026">
        <property type="entry name" value="PseC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30244">
        <property type="entry name" value="PTHR30244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01041">
        <property type="entry name" value="DegT_DnrJ_EryC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000390">
        <property type="entry name" value="PLP_StrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03588">
        <property type="entry name" value="PseC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="188"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR000390-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000390-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="385" mass="42299" checksum="7EB2A4EDE3B53B24" modified="2005-10-11" version="1">MIPYGRQDISSADVEAVVDVLRSDFLTQGPMVPRFEETLARTVDAPEAVAFSSATAALHIACLALGLGPSDRLWTVPNTFVASANCGLYCGATVDFVDINAKTRNMDVEALASKLEAAERDGTLPKVVAPVDFAGTSADLAAIRRLADRHGFRILEDASHAVGGYYQNRPIGSCEFADVTVFSFHPVKIVTTGEGGAATTRSAEIAHRMRLLRNHGISRDPADMTAVPEGAWYYEQVMLGFNYRMTDIQAALGVRQFDRLAEFVARRQTIAARYHEGLAGLPVIRQFVPEGTLSALHLYVIELDQTVRRTRAEVFAAMRASGIGVNVHYIPVHLQPWYRALGFSRGDFPAAEKYYDFALTLPIHPNLSVDQQDYVMERLSYALEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV00</accession>
    <name>Q3SV00_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Heat shock protein DnaJ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0624</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313951.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0624"/>
    <dbReference type="EnsemblBacteria" id="ABA03891">
        <property type="protein sequence ID" value="ABA03891"/>
        <property type="gene ID" value="Nwi_0624"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0624"/>
    <dbReference type="eggNOG" id="COG0484">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017633_0_0_5"/>
    <dbReference type="OMA" id="RVCPTCV"/>
    <dbReference type="OrthoDB" id="1738789at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="SSF46565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="SSF49493"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="3"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="69"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="316" mass="33832" checksum="C8CBE816E2123460" modified="2005-10-11" version="1">MRDPYEVLGVQRGASAAAIKSAFRKLAKKHHPDANKNDPKAAERFAELNSANEILGDEVRRKQFDRGEIDADGKPRFQGFPGGGGPRGQRPGGGFEQHGFRGGGPGFDGGFEDILNSMFGGARGGRRPGAQPFDFESSTFGTPDLDLNVAMTVTLEESLKGGEKRIRLPSGKELNVRIPPGVTSGQQIRLRGQGETVPGHRPGDLLITVTVAPHPFFKVDGSDLRAELPITLYESVLGGKIRVPTLGGAVELSIPKNTSGGRTFRLKGKGIPKGGVTGDLFVTTRIVLPDGNDAELEALMEKWRDARSYNPRGDLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="128" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVA4</accession>
    <name>Q3SVA4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_0520</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313848.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0520"/>
    <dbReference type="EnsemblBacteria" id="ABA03787">
        <property type="protein sequence ID" value="ABA03787"/>
        <property type="gene ID" value="Nwi_0520"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0520"/>
    <dbReference type="eggNOG" id="COG0643">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2198">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000650_5_2_5"/>
    <dbReference type="OMA" id="NELVECC"/>
    <dbReference type="OrthoDB" id="305000at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00088">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004105">
        <property type="entry name" value="CheA-like_dim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037006">
        <property type="entry name" value="CheA-like_homodim_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036061">
        <property type="entry name" value="CheW-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002545">
        <property type="entry name" value="CheW-lke_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036641">
        <property type="entry name" value="HPT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008207">
        <property type="entry name" value="Sig_transdc_His_kin_Hpt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01584">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02895">
        <property type="entry name" value="H-kinase_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01627">
        <property type="entry name" value="Hpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00260">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01231">
        <property type="entry name" value="H-kinase_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00073">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47226">
        <property type="entry name" value="SSF47226"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50341">
        <property type="entry name" value="SSF50341"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50851">
        <property type="entry name" value="CHEW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50894">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HPt" evidence="9">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="6">
        <location>
            <begin position="222"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="CheW-like" evidence="8">
        <location>
            <begin position="463"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="7">
        <location>
            <begin position="765"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="170"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphohistidine" evidence="3">
        <location>
            <position position="44"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="4">
        <location>
            <position position="815"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA03787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="888" mass="96242" checksum="AC023A4D65D51E72" modified="2005-10-11" version="1">MDDLLCEFLTETNESLDTVDNQLVRFEQDPNNAKILDNIFRLVHTIKGTCGFLGLPRLEALAHAAETLMGKFRDGMAVTGEAVTLILSTIDRIKDILGQLEASGTEPEGADADLIDALHRMAERDPSGPASDISEATPECDIAGTLTYQVLERPLRPGEVSLDDLERAFRETEVESTAPASQPAPAAPVASKPVATAAPKKVEDTPEGDRVANQSIRVNVDTLEHLMTMVSELVLTRNQLLEISRRHEDTEFKVPLQRLSNVTAELQEGVMKTRMQPIGNAWQKLPRIVRDLSSELGKQIELEMHGADTELDRQVLDLVKDPLTHMVRNSADHGLETPAERIKAGKPEQGTIRLSAYHEGGHIIICIADNGRGLDTERIKAKAIQNGLATEAEIERMTEAQIHKFIFAPGFSTAAAVTSVSGRGVGMDVVRTNIDQIGGTIDIKSVAGEGSSVIIKIPLTLAIVSALIVEAAGDRFAIPQLSVVELVRARANSEHRIERIRDTPVLRLRNKLLPLMHLKRLLKIDDGADSDPENGFIVVTQVGNQTFGIVVDGVFHTEEIVVKPMSTMLRHIGMFSGNTILGDGAVIMIIDPNGIAHALGASVVTQQEIADENAARAGAAEQTSLLVFRAGSDQPKAVPLALVTRLEEIAVAKIEISDGRHMVQYREQLMPLVQMDGVSIGTDGTQPVLVFADDGRAAGLVVDEIIDIVEERLNIEVASSRPGILGSAIIKGLATDVIDIGHFLPMAFSDWFRRKEMRAAATARSVLLVDDSAFFRNMLAPVLKAAGYQVRLATNAQEGLLALRSGQEFDAILTDIEMPDMNGFEFAETVRSDDRLSGTPIIALSSMISPAAIERGRQAGFHDYVAKFDRPGLIAALKEQTAEIGRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVB7</accession>
    <name>Q3SVB7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphate transport system permease protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_0507</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Part of the binding-protein-dependent transport system for phosphate; probably responsible for the translocation of the substrate across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cell inner membrane</location>
            <topology evidence="2 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0507"/>
    <dbReference type="EnsemblBacteria" id="ABA03774">
        <property type="protein sequence ID" value="ABA03774"/>
        <property type="gene ID" value="Nwi_0507"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0507"/>
    <dbReference type="eggNOG" id="COG0573">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033621_1_3_5"/>
    <dbReference type="OMA" id="ETMALYM"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011864">
        <property type="entry name" value="Phosphate_PstC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02138">
        <property type="entry name" value="phosphate_pstC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="100"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="137"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="310"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="100"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA03774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="341" mass="36350" checksum="E2F9D5F99B04EDA5" modified="2005-10-11" version="1">MVCKKQGWGVAEMAVHSSAIEQAASHDRSRAMDAFKLGDRVFYWITRISAILVLVLLGGIIMSLIVGSWPAIMEFGSSFLTTQRWAPSAEPPVLGALSPLYGTILTSIIAMSIAIPVGIGIAVFLTELCPLWARGPIGMAIELLAGIPSIIYGMWGFFILGPILADTVQPFFIDLFDGVPVLGSIFGGPPSYLSLFNASLILAIMVLPFITAISRDVFTTVPPVLKEAAYGVGCTTWEVVRHVVLPYTRVGVIGGAMLALGRALGETMAVTFIIGNSFRISPSIFAPGTTISAAIASEFAESDGLHQSSLMLLGLLLFVLTFFVLAAARLMLLRLEKKAGK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVD4</accession>
    <name>MUTS_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA mismatch repair protein MutS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mutS</name>
        <name type="ordered locus">Nwi_0490</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein is involved in the repair of mismatches in DNA. It is possible that it carries out the mismatch recognition step. This protein has a weak ATPase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVD4"/>
    <dbReference type="SMR" id="Q3SVD4"/>
    <dbReference type="STRING" id="323098.Nwi_0490"/>
    <dbReference type="EnsemblBacteria" id="ABA03757">
        <property type="protein sequence ID" value="ABA03757"/>
        <property type="gene ID" value="Nwi_0490"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0490"/>
    <dbReference type="eggNOG" id="COG0249">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002472_4_0_5"/>
    <dbReference type="OMA" id="TPMMAQY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00096">
        <property type="entry name" value="MutS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005748">
        <property type="entry name" value="DNA_mismatch_repair_MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017261">
        <property type="entry name" value="DNA_mismatch_repair_MutS/MSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007861">
        <property type="entry name" value="DNA_mismatch_repair_MutS_clamp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007860">
        <property type="entry name" value="DNA_mmatch_repair_MutS_con_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="PTHR11361"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05188">
        <property type="entry name" value="MutS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05190">
        <property type="entry name" value="MutS_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037677">
        <property type="entry name" value="DNA_mis_repair_Msh6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="SSF48334"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53150">
        <property type="entry name" value="SSF53150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55271">
        <property type="entry name" value="SSF55271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01070">
        <property type="entry name" value="mutS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000224385" description="DNA mismatch repair protein MutS">
        <location>
            <begin position="1"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="249"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="638"/>
            <end position="645"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="888" mass="95772" checksum="3824B5221D177F8C" modified="2005-10-11" version="1">MMEQYLEIKAANPGLLLFYRMGDFYELFFEDAEIASRALGITLTKRGKHQGADIPMCGVPVERSDDYLHRLIAAGHRVAVCEQTEDPSAARKRGNKSVVRRDVVRLVTPGTLTEDTLLDARANNYLLALARARASSGVDRFALAWIDISTAEFMVTECGANELAATLARINPNEVIVSDTLHGDPDLGALLRELPSVTPLPRDTFDGATAERRLCDYFAVATMDGLSAMSRLEATAAAAAVTYIDRTQIGQRPPLSPPSREASGSTMAIDPATRANLELTRTLAGERRGSLLDAIDRTMTAAGSRLLAQRLAAPLTDTAAITRRLDAVAALTADGAARDDIRAILRTAPDMSRALARLSLGRGGPRDLAGLRDGVMAADQTLARLAGLSDPPEGIAAAMQALRGPSRELARELGNALSENLPLMKRDGGFVREGYDTALDEARKLRDDSRLVVAAMQARYAEETGVKTLKIRHNNVLGYFVEVTAQHANRLMSAPLNATFIHRQTLAGQVRFTTSELGEIEARIANAGERALGLELDIFDRLAAMAIRNGDEIRNAAHAFAQLDVAASFAKLAIDENYTRPDIDASLSFAIEGGRHPVVEQALKRTGQPFIANACDLSPPPPPVEGSGESGQIWLLTGPNMAGKSTFLRQNALIALMAQVGSFVPASRARIGIIDRLFSRVGAADDLARGRSTFMVEMVETAVILNQASERALVILDEIGRGTATFDGLSIAWATIEHLHESNRCRALFATHYHELTALSAKLPRLFNATVRVKEWHGEVVFLHEVLPGAADRSYGIQVARLAGLPPSVIARAKSVLAKLEAQDRGSTVRVLVDDLPLFAVTSRTGESAPTGETSPLIEALKSLHPDEMSPREALDALYALKAKLPKQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVF5</accession>
    <name>GPDA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycerol-3-phosphate dehydrogenase [NAD(P)+]</fullName>
            <ecNumber evidence="1">1.1.1.94</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">NAD(P)H-dependent glycerol-3-phosphate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gpsA</name>
        <name type="ordered locus">Nwi_0469</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NAD(+) + sn-glycerol 3-phosphate = dihydroxyacetone phosphate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:11092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.94"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NADP(+) + sn-glycerol 3-phosphate = dihydroxyacetone phosphate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:11096"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.94"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.94" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313800.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVF5"/>
    <dbReference type="SMR" id="Q3SVF5"/>
    <dbReference type="STRING" id="323098.Nwi_0469"/>
    <dbReference type="EnsemblBacteria" id="ABA03736">
        <property type="protein sequence ID" value="ABA03736"/>
        <property type="gene ID" value="Nwi_0469"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0469"/>
    <dbReference type="eggNOG" id="COG0240">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033449_0_2_5"/>
    <dbReference type="OMA" id="NRMFGNM"/>
    <dbReference type="OrthoDB" id="1419877at2"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009331">
        <property type="term" value="C:glycerol-3-phosphate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004367">
        <property type="term" value="F:glycerol-3-phosphate dehydrogenase [NAD+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046167">
        <property type="term" value="P:glycerol-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046168">
        <property type="term" value="P:glycerol-3-phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006650">
        <property type="term" value="P:glycerophospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00394">
        <property type="entry name" value="NAD_Glyc3P_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006168">
        <property type="entry name" value="G3P_DH_NAD-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006109">
        <property type="entry name" value="G3P_DH_NAD-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011128">
        <property type="entry name" value="G3P_DH_NAD-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07479">
        <property type="entry name" value="NAD_Gly3P_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01210">
        <property type="entry name" value="NAD_Gly3P_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000114">
        <property type="entry name" value="Glycerol-3-P_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00077">
        <property type="entry name" value="GPDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00957">
        <property type="entry name" value="NAD_G3PDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000255337" description="Glycerol-3-phosphate dehydrogenase [NAD(P)+]">
        <location>
            <begin position="1"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="190"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="11"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="254"/>
            <end position="255"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00394"/>
        </source>
    </evidence>
    <sequence length="326" mass="32878" checksum="302AA4182D6B39C5" modified="2005-10-11" version="1">MKPLNRICVIGAGAWGTALAGVAARAGREVTLYARDAAAAAQISATRDNPRLPGIPLDRGIVVTHDIAAAGSSDIVIIAAPAQHSRTAITTLAPHLSPGTPVIASAKGIEHGTRRFMTEVIEEAAPHAVPAILSGPSFADDVARGLPTAVTLAAKDEALAIALVHALGSAAFRPYHTTDVRGVEIGGAAKNVLAIAAGIVVGKQLGASALAALTTRGFSELVRLGCACGGRGETLAGLSGLGDLILTCSSPQSRNFSLGIALGRGDPAPRDKLSEGAHTAPALLELAAARDVEMPVAQAVAAILAGRLTIDGAIDNLLTRPFRGEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVM1</accession>
    <name>Q3SVM1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cobaltochelatase CobS subunit</fullName>
            <ecNumber evidence="3">6.6.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0403</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.6.1.2" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313734.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0403"/>
    <dbReference type="EnsemblBacteria" id="ABA03670">
        <property type="protein sequence ID" value="ABA03670"/>
        <property type="gene ID" value="Nwi_0403"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0403"/>
    <dbReference type="eggNOG" id="COG0714">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051316_1_0_5"/>
    <dbReference type="OMA" id="QVTEFQE"/>
    <dbReference type="OrthoDB" id="895310at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051116">
        <property type="term" value="F:cobaltochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025865">
        <property type="entry name" value="CobS_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006537">
        <property type="entry name" value="PD_CobS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12556">
        <property type="entry name" value="CobS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01650">
        <property type="entry name" value="PD_CobS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobS_N" evidence="2">
        <location>
            <begin position="11"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="AAA_5" evidence="1">
        <location>
            <begin position="71"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="332" mass="37108" checksum="1FB0A51DB9614BD4" modified="2005-10-11" version="1">MTTAAITNTETPTGLPDMKVSVRQVFGIDSDLEVPAYSGTDPHVPDVDPDYRFDRATTLAILAGFAKNRRVMVTGYHGTGKSTHIEQVAARLNWPCVRVNLDSHISRIDLVGKDAIVLKDGKQVTEFRDGILPWAYQNNVALCFDEYDAGRPDVMFVIQRVLESSGRLTLLDQSRVIKPHPAFRLFATANTIGLGDTSGLYHGTQQINQAQMDRWNIVTTLNYLPHDNEVEIVLAKAKHYRTPEGRDIVGKMVRLADLTRNAFANGDLSTVMSPRTVITWAENTEIFDDLGFAFRVTFLNKCDELERPLVAEFYQRCFNVELSESAVNVALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVQ2</accession>
    <name>Q3SVQ2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cupin_5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0372</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0372"/>
    <dbReference type="EnsemblBacteria" id="ABA03639">
        <property type="protein sequence ID" value="ABA03639"/>
        <property type="gene ID" value="Nwi_0372"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0372"/>
    <dbReference type="eggNOG" id="COG3542">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088365_1_1_5"/>
    <dbReference type="OMA" id="ERSHWHR"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06121">
        <property type="entry name" value="cupin_YML079wp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009327">
        <property type="entry name" value="Cupin_DUF985"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039935">
        <property type="entry name" value="YML079W-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33387">
        <property type="entry name" value="PTHR33387"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06172">
        <property type="entry name" value="Cupin_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin_5" evidence="1">
        <location>
            <begin position="9"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="135" mass="14877" checksum="E326088E3818CD81" modified="2005-10-11" version="1">MDMALSATDIIERLDLQPHPEGGHYRETFRDTRTDGHGRAASTAIYFLLARGERSQRHRIDAVEVWHYYAGSPLLLRIEDGAVTRTLRLGADLAAGETPQALVPAFAWQSAESTGDWTLVGCTVAPGFEFSGFEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVV6</accession>
    <name>Q3SVV6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Periplasmic protein thiol-disulfide oxidoreductase DsbE</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0318</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioredoxin family. DsbE subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313650.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0318"/>
    <dbReference type="EnsemblBacteria" id="ABA03585">
        <property type="protein sequence ID" value="ABA03585"/>
        <property type="gene ID" value="Nwi_0318"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0318"/>
    <dbReference type="eggNOG" id="COG0526">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_19_0_5"/>
    <dbReference type="OMA" id="ANYIPIT"/>
    <dbReference type="OrthoDB" id="1617952at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030288">
        <property type="term" value="C:outer membrane-bounded periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015036">
        <property type="term" value="F:disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03010">
        <property type="entry name" value="TlpA_like_DsbE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004799">
        <property type="entry name" value="Periplasmic_diS_OxRdtase_DsbE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013740">
        <property type="entry name" value="Redoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08534">
        <property type="entry name" value="Redoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00385">
        <property type="entry name" value="dsbE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="6">
        <location>
            <begin position="46"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="197" mass="21395" checksum="9BAD3B1D7D86279B" modified="2005-10-11" version="1">MTDPEPPAAAPKRRWLMALPLVLFAALALLFWFRLGDRDISRIPSALIGRAAPQIPLPPLSGLMADGKPVPGIDPATFVGKVSLVNVWASWCVPCHSEAPLLTEFAKDRRVQFVGINYKDASDNARRFLGRYGNPFDVVGVDSNGRASIEWGVYGVPETFVVARDGTIAYKLVGEITPENIEAVLKPEIEKALKAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVW8</accession>
    <name>Q3SVW8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flp pilus assembly protein TadG</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0306</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313639.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0306"/>
    <dbReference type="EnsemblBacteria" id="ABA03573">
        <property type="protein sequence ID" value="ABA03573"/>
        <property type="gene ID" value="Nwi_0306"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0306"/>
    <dbReference type="eggNOG" id="COG4961">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111553_2_1_5"/>
    <dbReference type="OMA" id="RPYITTI"/>
    <dbReference type="OrthoDB" id="1780812at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012495">
        <property type="entry name" value="TadE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07811">
        <property type="entry name" value="TadE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="186" mass="19880" checksum="1ABB6676E5137FDC" modified="2005-10-11" version="1">MSSLRRLKATARMAARFPGHKSGSAAVEFALVAPMFFALLFAIIETGLVFFASQSLETALQDSARTIMTGEAQITNLTKQSFKANVVCANVNVLFDCENGIYVDVQSYPSGFGSVTISDPIAGGSFVDNTQYNPGGPGDIVVVRLFYQWPLYVTGLGYNIANLSGSKRLLSATAAFKNEPYPAPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW10</accession>
    <name>Q3SW10_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0264</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313597.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0264"/>
    <dbReference type="EnsemblBacteria" id="ABA03531">
        <property type="protein sequence ID" value="ABA03531"/>
        <property type="gene ID" value="Nwi_0264"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0264"/>
    <dbReference type="eggNOG" id="COG0603">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_593036_0_0_5"/>
    <dbReference type="OMA" id="RAWDFLS"/>
    <dbReference type="OrthoDB" id="1861107at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008616">
        <property type="term" value="P:queuosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018317">
        <property type="entry name" value="QueC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06508">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0671">Queuosine biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="452" mass="49932" checksum="B6DA945828D04DC4" modified="2005-10-11" version="1">MTATAFQPRIPDKAIDVVEPGRRARKGHLVCQLGRDLSFSTAALEHYAFARWEPVIYDAMLVAAAVEYADRSIRRPSLGWTRKIALRVPVHDPGRWSDPGVFTALHEALRFLTGDIWDLEFIKRRSAAPKPQQEFLQLPVKTEAVIAYSDGMDSRAVAGLMAESLGERLVRVRVGPKAGGRPFKGQREPFAAVPYSVAATGETSGRSRGFKFALISGIAAYLAEADVILVPESGQGAFGPALVTVAHAYPDYRNHPLFTSRMERFLNALFRRPVRFVFPRLWHTKAETLREYAALPGSKWDDTKSCWRNARWSALNGNLVQCGICAACMLRRMSIHTAGLVDGPDAYVCNDLTAATIEEAVHPGFKKVNSAFREYAIAGTLHLDHLADMAQPNNHAALKRHAALTSTALGMTAAEAEGRLIGLLERHAAEWNKFTDDLGARSFIKKWTRASQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW12</accession>
    <name>Q3SW12_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AbiEi_1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0262</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313595.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0262"/>
    <dbReference type="EnsemblBacteria" id="ABA03529">
        <property type="protein sequence ID" value="ABA03529"/>
        <property type="gene ID" value="Nwi_0262"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0262"/>
    <dbReference type="eggNOG" id="COG5340">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084420_1_0_5"/>
    <dbReference type="OMA" id="WYIDALM"/>
    <dbReference type="OrthoDB" id="1786226at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025159">
        <property type="entry name" value="AbiEi_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018547">
        <property type="entry name" value="AbiEi_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09407">
        <property type="entry name" value="AbiEi_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13338">
        <property type="entry name" value="AbiEi_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AbiEi_1" evidence="1">
        <location>
            <begin position="73"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="266" mass="28549" checksum="06CAB35702F46278" modified="2005-10-11" version="1">MKSEKSLHARDYITALAASGRYLFSSKEAQAALGVSADAAKLALNRLGKRGLIAAPARGFYVIVPPEYQSLRCLPAEQFIPDLMKRLGAAYYTGLLSAAQFHGAAHHRPQEFQVMVAKARRTIVCGAVRVAFFVRKNLSAIPVQSFNTPRGTILVSSPEATAFDLVGYQGQIGGLDQVATVLGELAEKLNPEKLADVAQSAPVPWAQRLGYLLGKVDAADRTEALRSFVSTHARQTAPLLPAAPLDGAARDDVWKLAINADVEAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW16</accession>
    <name>Q3SW16_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HNH_5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0258</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313591.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0258"/>
    <dbReference type="EnsemblBacteria" id="ABA03525">
        <property type="protein sequence ID" value="ABA03525"/>
        <property type="gene ID" value="Nwi_0258"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0258"/>
    <dbReference type="eggNOG" id="COG1403">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_684812_0_0_5"/>
    <dbReference type="OrthoDB" id="1472463at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029471">
        <property type="entry name" value="HNH_5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14279">
        <property type="entry name" value="HNH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HNH_5" evidence="1">
        <location>
            <begin position="7"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="402" mass="44451" checksum="4E5A7A3805A09CFF" modified="2005-10-11" version="1">MDNPLLCIFCDNALGPDTKPEHILPNALGGRKTTRRVICSDCNNRFGSTIDDALAKQVEIIRNLLQLESGTGKPPPMLRNVQAGTEKVTFRNDGQPELVTPPFTVTERPDGTAEVAITVKTPEELRRFLPHIAARLRMSEDELVKQMEGGTGVVVERRPGEVHHHISFGKEDAIRSVAKSCLVLLATLVGNDAVKSDAFKEARTFVLTGSKEFNEKRVTIDSRPVPGVDDLIRVYGMFFNLIYVRSDSAGRVIGHFTLYNAISWQIVLAEAGGPAECKSGLVSNPLDPTSWSDAIADTVNIPFAWLDAADKGYELERARQRLISMAQYHHDESQQAEIARIVNDVCAKYGIKSDNDPIDPAHQEAIFNEITARLAAHAMHQPYEQRLTADQMKKLLKGESSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW32</accession>
    <name>Q3SW32_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two component transcriptional regulator, LuxR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0241</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313575.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0241"/>
    <dbReference type="EnsemblBacteria" id="ABA03509">
        <property type="protein sequence ID" value="ABA03509"/>
        <property type="gene ID" value="Nwi_0241"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0241"/>
    <dbReference type="eggNOG" id="COG4566">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_4_5"/>
    <dbReference type="OMA" id="IMTGHGN"/>
    <dbReference type="OrthoDB" id="930905at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="12"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="142"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="216" mass="23403" checksum="EA18C91DC34C1912" modified="2005-10-11" version="1">MGKLLSFVSDVSIFILDDDPSVRETLVLLLKIEGYAAQGFGDGSSFLEAVRNASPACVILDLNLPGESGFDVLRRLADERVAAPILVISGAADISMAVAAMKYGALDFLEKPFSSDVIIERVRAAVKVWREARGSERDGFANFPGRHLLTCREREVLSQVARGASNKEAGRRLGISPRTVEVHRARIMDKLGAKNTADLIRIVFSEGSAWRGLSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW44</accession>
    <name>Q3SW44_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Threonine synthase</fullName>
            <ecNumber evidence="5">4.2.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_0229</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + O-phospho-L-homoserine = L-threonine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10840"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57590"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.2.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the threonine synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.1" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313564.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0229"/>
    <dbReference type="EnsemblBacteria" id="ABA03497">
        <property type="protein sequence ID" value="ABA03497"/>
        <property type="gene ID" value="Nwi_0229"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0229"/>
    <dbReference type="eggNOG" id="COG0498">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015170_1_0_5"/>
    <dbReference type="OMA" id="FGRIAFQ"/>
    <dbReference type="OrthoDB" id="428821at2"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00065"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004795">
        <property type="term" value="F:threonine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029144">
        <property type="entry name" value="Thr_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037158">
        <property type="entry name" value="Thr_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004450">
        <property type="entry name" value="Thr_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14821">
        <property type="entry name" value="Thr_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00260">
        <property type="entry name" value="thrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Thr_synth_N" evidence="11">
        <location>
            <begin position="3"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="PALP" evidence="10">
        <location>
            <begin position="100"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="9">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR604450-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA03497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="472" mass="51188" checksum="6798872C1FDD8E22" modified="2005-10-11" version="1">MTHYLSTRGENQKLGFSDVMLAGLARDGGLYVPEVWPQLRLDTVAAFFGRPYSEVAVEVIRPFVGGDIADVDLARMANEAYATFRHPAVVPLDQIGSQQFLLELFHGPTLAFKDVAMQLLSRLMDHVLARRGQRTTIVVATSGDTGGAAVDAFANRANVDLIALFPDGRISEVQRRMMTTTAAPNVHALAVKGTFDDCQAIVKGLFNNHRFRDEVSLSGVNSINWARIVAQVVYYFTSAVALGAPARAVDFTVPTGNFGDIFAGYVAKRMGLPVRRLRIAANINDILPRTLKTGIYEVREVHATSSPSMDIQVSSNFERLLFEATGRDAAQVRALMASLQQSGRFALPESVLAAMRADFDAECADETETAATIRTAWREAGDLLDPHTAVALTVADRDAAESRVPNIVLATAHAAKFPDAVEAACGVRPPLPAWLDGLMTRPERITIVNNDQAEIERFVLSVSRAANHGVTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="131" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW57</accession>
    <name>Q3SW57_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tryptophan--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Tryptophanyl-tRNA synthetase</fullName>
            <shortName evidence="8">TrpRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">trpS</name>
        <name evidence="10" type="ordered locus">Nwi_0216</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the attachment of tryptophan to tRNA(Trp).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + H(+) + L-tryptophanyl-tRNA(Trp)</text>
            <dbReference type="Rhea" id="RHEA:24080"/>
            <dbReference type="Rhea" id="RHEA-COMP:9671"/>
            <dbReference type="Rhea" id="RHEA-COMP:9705"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78535"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 9">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.2" evidence="8"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313551.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0216"/>
    <dbReference type="EnsemblBacteria" id="ABA03484">
        <property type="protein sequence ID" value="ABA03484"/>
        <property type="gene ID" value="Nwi_0216"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0216"/>
    <dbReference type="eggNOG" id="COG0180">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029244_1_4_5"/>
    <dbReference type="OMA" id="GWGQFKP"/>
    <dbReference type="OrthoDB" id="951354at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004830">
        <property type="term" value="F:tryptophan-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006436">
        <property type="term" value="P:tryptophanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00806">
        <property type="entry name" value="TrpRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00140_B">
        <property type="entry name" value="Trp_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002306">
        <property type="entry name" value="Trp-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024109">
        <property type="entry name" value="Trp-tRNA-ligase_bac-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01039">
        <property type="entry name" value="TRNASYNTHTRP"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00233">
        <property type="entry name" value="trpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="short sequence motif" description="'HIGH' region" evidence="8">
        <location>
            <begin position="11"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="8">
        <location>
            <begin position="208"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="10"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="18"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>L-tryptophan</name>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="150"/>
            <end position="152"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="208"/>
            <end position="212"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA03484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="347" mass="38017" checksum="ED7EF17C7D52172A" modified="2005-10-11" version="1">MTTRVFSGVQPTGNLHLGNYLGAIVNFVKLQADHECLYCVVDLHAITVPVAVWGGPDELRRATREVTAAFIASGIDPRQHIIFNQSQVAGHAELAWVLNCVARLGWLNRMTQFKEKAGKDRENASVGLYDYPVLMASDILVYRATHVPVGEDQKQHLELSRDIAQKFNNDFSESIAANGHGDMFFPLPEPVITGPATRVMSLRDGTKKMSKSDPSDYSRINLTDNADTIAQKIRKAKTDPEPLPSEEKGLENRPEADNLVGIYAALSGKPKSALLAEFGGAQFSSFKSSLVDLAVAKLSPIAHEMKRLTDDPAHVDRILIEGAERASAIASQTMDAVKDIVGFIRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWC5</accession>
    <name>Q3SWC5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0148</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313485.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03416">
        <property type="protein sequence ID" value="ABA03416"/>
        <property type="gene ID" value="Nwi_0148"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0148"/>
    <dbReference type="HOGENOM" id="CLU_1711321_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="153" mass="17393" checksum="3C851A57FEF3077F" modified="2005-10-11" version="1">METLREFITRRHKELDELEAPLRQQLAAIADEREELRRAALAAGPPPMRPANTVTQQADEGRRSRRTIPEKTIKDAVVEVLRAKGSGMTALEILAAINLKFGTEYPRTSLSPQLSRLKADGKINRDGIVWTLEKKLIDYVDQPFQRDIEEAFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWH3</accession>
    <name>Q3SWH3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcription termination factor Rho</fullName>
            <ecNumber evidence="8">3.6.4.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">ATP-dependent helicase Rho</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rho</name>
        <name evidence="11" type="ordered locus">Nwi_0100</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homohexamer. The homohexamer assembles into an open ring structure.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the Rho family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="8"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313437.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0100"/>
    <dbReference type="EnsemblBacteria" id="ABA03368">
        <property type="protein sequence ID" value="ABA03368"/>
        <property type="gene ID" value="Nwi_0100"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0100"/>
    <dbReference type="eggNOG" id="COG1158">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016377_4_3_5"/>
    <dbReference type="OMA" id="TRLCRAH"/>
    <dbReference type="OrthoDB" id="1619125at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008186">
        <property type="term" value="F:ATP-dependent activity, acting on RNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04459">
        <property type="entry name" value="Rho_CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01128">
        <property type="entry name" value="rho_factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01884">
        <property type="entry name" value="Rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041703">
        <property type="entry name" value="Rho_factor_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011112">
        <property type="entry name" value="Rho_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036269">
        <property type="entry name" value="Rho_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011113">
        <property type="entry name" value="Rho_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004665">
        <property type="entry name" value="Term_rho"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46425">
        <property type="entry name" value="PTHR46425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07498">
        <property type="entry name" value="Rho_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07497">
        <property type="entry name" value="Rho_RNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00959">
        <property type="entry name" value="Rho_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68912">
        <property type="entry name" value="SSF68912"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00767">
        <property type="entry name" value="rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51856">
        <property type="entry name" value="RHO_RNA_BD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 8" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="Rho RNA-BD" evidence="10">
        <location>
            <begin position="51"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="172"/>
            <end position="177"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="184"/>
            <end position="189"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA03368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="421" mass="47178" checksum="5B95ABA7AE918986" modified="2005-10-11" version="1">MREMKLQDLKAKTPAELVSFAEELGVENASTMRKQELMFAILKQLAIQETDIIGEGVVEVLSDGFGFLRSPDANYLPGPDDIYVSPSQIRRFGLRTGDTIEGHIRSPKEGERYFALLKVNTLNFEDPEKSKHKVNFDNLTPLFPDQRFRLELEDPTRKDLSARVIDIVAPIGKGQRALIVAPPRTGKTVLMQNIAHSITANHPECYLIVLLIDERPEEVTDMQRSVKGEVVSSTFDEPAVRHVQVAEMVIEKAKRLVEHGRDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIATALVDTGSRMDEVIFEEFKGTGNSELILDRKVADKRTFPAIDIARSGTRKEELITDPQLLKKMYVLRRILNPMGTMDAIEFLLDKLRNTKNNSEFFESMNT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWH7</accession>
    <name>RSMG_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal RNA small subunit methyltransferase G</fullName>
            <ecNumber evidence="1">2.1.1.170</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">16S rRNA 7-methylguanosine methyltransferase</fullName>
            <shortName evidence="1">16S rRNA m7G methyltransferase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rsmG</name>
        <name type="ordered locus">Nwi_0096</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically methylates the N7 position of guanine in position 527 of 16S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>guanosine(527) in 16S rRNA + S-adenosyl-L-methionine = N(7)-methylguanosine(527) in 16S rRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42732"/>
            <dbReference type="Rhea" id="RHEA-COMP:10209"/>
            <dbReference type="Rhea" id="RHEA-COMP:10210"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74480"/>
            <dbReference type="EC" id="2.1.1.170"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. RNA methyltransferase RsmG family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.170" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWH7"/>
    <dbReference type="SMR" id="Q3SWH7"/>
    <dbReference type="STRING" id="323098.Nwi_0096"/>
    <dbReference type="EnsemblBacteria" id="ABA03364">
        <property type="protein sequence ID" value="ABA03364"/>
        <property type="gene ID" value="Nwi_0096"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0096"/>
    <dbReference type="eggNOG" id="COG0357">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065341_1_0_5"/>
    <dbReference type="OMA" id="ICFPHLH"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070043">
        <property type="term" value="F:rRNA (guanine-N7-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00074">
        <property type="entry name" value="16SrRNA_methyltr_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003682">
        <property type="entry name" value="rRNA_ssu_MeTfrase_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31760">
        <property type="entry name" value="PTHR31760"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02527">
        <property type="entry name" value="GidB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00138">
        <property type="entry name" value="rsmG_gidB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000335384" description="Ribosomal RNA small subunit methyltransferase G">
        <location>
            <begin position="1"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="188"/>
            <end position="189"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00074"/>
        </source>
    </evidence>
    <sequence length="277" mass="30419" checksum="EAC4CACBBFB3C060" modified="2005-10-11" version="1">MFHVKHPQREVAATRQRAVGQWPTDPYPRRLNVWPGIRDASMLDGMSAPLQKYSPCSATDKAAALALTPVSSETEARLDAYVDLLVQWQTKTNLVASSTLPQLWTRHIADSLQLLALAPQAKCWADLGSGGGFPGVVLACALADIEGARVDLVERNAKKAAFLREAVRITASPASVHLMDIGDYVDRSEDRIDCVTARALAPLQVLLGFVEPLVNKGAKALFLKGQDVEAELTEATRYWNIEPRIHSSCTGGQGRIAEFDHIERRDRRGARQAWRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWI6</accession>
    <name>Q3SWI6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Two component transcriptional regulator, winged helix family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_0087</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313424.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0087"/>
    <dbReference type="EnsemblBacteria" id="ABA03355">
        <property type="protein sequence ID" value="ABA03355"/>
        <property type="gene ID" value="Nwi_0087"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0087"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_3_5"/>
    <dbReference type="OMA" id="EMGSDDY"/>
    <dbReference type="OrthoDB" id="817710at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="6"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="5">
        <location>
            <begin position="129"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="3">
        <location>
            <begin position="129"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA03355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="228" mass="25062" checksum="7AFADAFB392C701E" modified="2005-10-11" version="1">MANARKILIVDDDNDLRDTLVEQLSLHEEFEASAVDTGAKGASAARSCSPDLVLMDVGLPDTDGREVVRSLRKGGFKAPIIMLTGHDTDSDTILGLESGANDYIAKPFRFAVLLARIRAQLRQHEASEDAVFSVGPYSFRPGAKMLTTGNGKKVRLTEKETAILRFLYRAGQMSVSRDTLLQEVWGYNSGVTTHTLETHIYRLRQKIEKDAANPEILVTEAGGYKLVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMZ9</accession>
    <name>Q3SMZ9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_3095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3095"/>
    <dbReference type="EnsemblBacteria" id="ABA06342">
        <property type="protein sequence ID" value="ABA06342"/>
        <property type="gene ID" value="Nwi_3095"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3095"/>
    <dbReference type="eggNOG" id="ENOG50339SA">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170277_0_0_5"/>
    <dbReference type="OMA" id="SCNDGRS"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004228980" evidence="1">
        <location>
            <begin position="21"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="85" mass="9381" checksum="C2AF1A62994A6D78" modified="2005-10-11" version="1">MRMLLTSLSVFVALAVPAKADRVVTETEGDKLISALTAQGCTGGKMEWDDDDREFEVGNASCNDGRSYDLKFNTDFTLKNKKLDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN44</accession>
    <name>Q3SN44_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ABC-type Fe3+-siderophore transport system permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_3048</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3048"/>
    <dbReference type="EnsemblBacteria" id="ABA06297">
        <property type="protein sequence ID" value="ABA06297"/>
        <property type="gene ID" value="Nwi_3048"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3048"/>
    <dbReference type="eggNOG" id="COG0609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013016_0_3_5"/>
    <dbReference type="OMA" id="SFRMPRI"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000522">
        <property type="entry name" value="ABC_transptr_permease_BtuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472">
        <property type="entry name" value="PTHR30472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01032">
        <property type="entry name" value="FecCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="30"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="256"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="300"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="328"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA06297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="358" mass="36444" checksum="61718ABC9DFB91B5" modified="2005-10-11" version="1">MGRRTGRTGGSPCFRGHMVIAPAAGRQRMIVTLALCAGVAALTLVSLGIGPVALSPTTILHALFGGGGDVQRIIVLEIRLPRAILAIVIGGILGLSGAALQGLVRNPLASPSLFGAPQAAAFGAVLLIALGWADIHSFALPVAAILAAFTSVFVLLVIAGRDTSLLILILAGLAISALAGAATSLTMNLSSNPFAALEIAFWLLGSLEDRSFRHVALALPFIAAGAATLMSQRHAFRTLSLGEEAALSLGVNVGRLRLWTILGVALGIGGAVAVSGAIGFIGLVAPHLMRPLIGYDPSRLLVPSALTGAVLLLAADIAVRVIPSTSDIKVGVLTALIGVPFFLYLIVRERRALGGGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN60</accession>
    <name>Q3SN60_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acriflavin resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_3031</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316199.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3031"/>
    <dbReference type="EnsemblBacteria" id="ABA06281">
        <property type="protein sequence ID" value="ABA06281"/>
        <property type="gene ID" value="Nwi_3031"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3031"/>
    <dbReference type="eggNOG" id="COG0841">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002755_1_2_5"/>
    <dbReference type="OMA" id="IFAPASF"/>
    <dbReference type="OrthoDB" id="145346at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2090.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027463">
        <property type="entry name" value="AcrB_DN_DC_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001036">
        <property type="entry name" value="Acrflvin-R"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32063">
        <property type="entry name" value="PTHR32063"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00873">
        <property type="entry name" value="ACR_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00702">
        <property type="entry name" value="ACRIFLAVINRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82714">
        <property type="entry name" value="SSF82714"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="360"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="389"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="431"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="463"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="510"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="845"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="871"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="898"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="943"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="974"/>
            <end position="999"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1042" mass="112030" checksum="35199D3646FD9D6F" modified="2005-10-11" version="1">MASNISAWSIRNPLPSVLFSIILLILGWVSFTNLAITRLPSADIPVISVAVSQFGAAPAELEAQVTKTIEDGVSGVEGVRHISSSITDGLSLTTIQFALETNTDRALNDVKDAVTRVRANLPQNVNEPLIQRVDVIGLPIVTYAAISPGKTPEQLSYFVDDVVKRTLQGVRGVAQVERIGGVEREILVSLDPDRLHAVGLTAVDVSQRLRGINVDVAGGRAEIGQNDQAIRTLAGAKTLNELAGTMISLPGGSEIRLDDLGTVTDTIADRRTFARFNGEPVVALGIKRSKGASDVVVAEAVQERINALKEQYPEVDLKLIDTSVEFTKGNYEAAISTLFEGATLAVIIVFLFLRDIRATIIAAISLPLSIFPAFWAMDILGFSLNLVSFLAITLSTGILVDDAIVEIENIVRHMRMGKSPYRAALEAADEIGLAVIAISLTIIAIFAPASFMSGIAGQFFKQFGITVSVQVFFSLLAARLVTPVLAAYFMKDHPHEAPPPGRVLRAYTRLVTWSVRHYIITVLIGFAVFAASIWSIKLLPQGFLPAQDTARSLLAIELPPGSQLAHTEKVTEGIAARLRKRPEVTSVFVDGGRVPPGLQEVRRASLIINYTPKQDRGITQRELELQIGQDLESVPDVRYWFLDENGLRAVALVVTGPDIGIVSNVANELATQMKRIPLIANVTPETSLDRPELRIRPRADLAARLGVSTEQLSQTIRVATIGDVGPALAKFDAGDRQVPIRVQLEDGARADLKVLEQMRVPIGGGRGHVPLSVVADIRLDQGPTSINRYDRERQATVAADLVGTAALGDATDMINDLPVMKSLPKGVRVNPSGDAESLNELSEGFATAITAGLMMVYAVLVLLFGTFLQPITILFSLPLSIGGAIMALLLTGKQLTTPVWIGILMLMGIVTKNAIMLVEFAIESIREGRAREEAMIDAGMKRARPIVMTTVAMVAGMMPSALAFGAGGEFRSPMALAVIGGLLFSTILSLVFVPAMFLLMDDVGKFIWRHSKRVLTSGAEIAQPETAEHKKPTSAVPLPGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN94</accession>
    <name>Q3SN94_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CsbD-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2997</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0337 (CsbD) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316169.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2997"/>
    <dbReference type="EnsemblBacteria" id="ABA06247">
        <property type="protein sequence ID" value="ABA06247"/>
        <property type="gene ID" value="Nwi_2997"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2997"/>
    <dbReference type="eggNOG" id="COG3237">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135567_3_2_5"/>
    <dbReference type="OMA" id="KAKGMAN"/>
    <dbReference type="OrthoDB" id="2089955at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008462">
        <property type="entry name" value="CsbD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036629">
        <property type="entry name" value="YjbJ_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05532">
        <property type="entry name" value="CsbD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69047">
        <property type="entry name" value="SSF69047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CsbD" evidence="2">
        <location>
            <begin position="6"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="71" mass="7155" checksum="55A4BB188519917E" modified="2005-10-11" version="1">MGSTTDKIKGTANEAIGKAKQVIGEATGSEKLQGEGAIQELKGKGQNALGDAKQATKDAADKAAATINKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNE7</accession>
    <name>Q3SNE7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative bacteriophage-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2944</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2944"/>
    <dbReference type="EnsemblBacteria" id="ABA06194">
        <property type="protein sequence ID" value="ABA06194"/>
        <property type="gene ID" value="Nwi_2944"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2944"/>
    <dbReference type="eggNOG" id="ENOG50332DQ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124387_1_0_5"/>
    <dbReference type="OMA" id="EYHEVEH"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021322">
        <property type="entry name" value="DUF2924"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11149">
        <property type="entry name" value="DUF2924"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="170" mass="18637" checksum="10BC5F0FE8A27DC8" modified="2005-10-11" version="1">MERRSGSRKPIGRRLMGLSMEDEIAHLRSLDLLSLHARWQSVVGRPAPEHLPKHLLFGILAYRIQADALGDLDAATVQLLKRAATVRSLSEVLPLVSAQDQRKQAMVPGTVLTREWNGQLHRVMAVEGGFAFEGKTFDSLSGIAFAITGTNWNGPRFFGLRAGNGKGVQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNI8</accession>
    <name>Q3SNI8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl hydrolase, BNR repeat</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2903</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316083.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2903"/>
    <dbReference type="EnsemblBacteria" id="ABA06153">
        <property type="protein sequence ID" value="ABA06153"/>
        <property type="gene ID" value="Nwi_2903"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2903"/>
    <dbReference type="eggNOG" id="COG4409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_658671_0_0_5"/>
    <dbReference type="OMA" id="SCECCRI"/>
    <dbReference type="OrthoDB" id="1293876at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004308">
        <property type="term" value="F:exo-alpha-sialidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011040">
        <property type="entry name" value="Sialidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026856">
        <property type="entry name" value="Sialidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036278">
        <property type="entry name" value="Sialidase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10628">
        <property type="entry name" value="PTHR10628"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13088">
        <property type="entry name" value="BNR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50939">
        <property type="entry name" value="SSF50939"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229109" evidence="1">
        <location>
            <begin position="21"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="Sialidase" evidence="2">
        <location>
            <begin position="64"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="402" mass="43414" checksum="5024587ADF13F216" modified="2005-10-11" version="1">MIRTAAAAAIALFLQGAANAQMSHHHASEAACDTTELRCATKVTPAFAPDGTLWLAWMAGGRISVARSGDHGKTFSAPVAVFDQALKLDWGPDARPSMIVDRQNRIAIAFSIFRDKNFNGEVLYTRSVDEGRRFSKPEPITPGTESQRFVQLALDSDGSVFAAWIDKRNRARASAGNQSYNGAALFFSWSKDGGGTYSETRLVKDNTCECCRIAVAFAGSRQPAVLFRNIFPGGVRDHAIVTFGDSAAPGEIHRVSHDGWKTDACPHQGPSLAISPEGTYHAVWFTSGEARKGLFHARSTDGGQTFSEPMAIGRPDRNASRPYVLTSGSGTTIVWKEFDGEKTTVKLIRSGDDGKSWSQPRDIAATTDASDHPLLVGNGSEVFLSWMTKADGYHITQIDSAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNM1</accession>
    <name>Q3SNM1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2870"/>
    <dbReference type="EnsemblBacteria" id="ABA06120">
        <property type="protein sequence ID" value="ABA06120"/>
        <property type="gene ID" value="Nwi_2870"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2870"/>
    <dbReference type="eggNOG" id="COG2333">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2437844_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="90" mass="10154" checksum="2297AD244E81A5A2" modified="2005-10-11" version="1">MTASLTFFPVSNGDMVLMCLDNGQRILIDINIRGKADDDDDDTPDVAEDLRSRLERDDQGRHPHSDHVAGLRNHFHLGHRTPGTKMMTRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNS7</accession>
    <name>Q3SNS7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2811</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06064">
        <property type="protein sequence ID" value="ABA06064"/>
        <property type="gene ID" value="Nwi_2811"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2811"/>
    <dbReference type="eggNOG" id="COG3189">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137928_0_0_5"/>
    <dbReference type="OMA" id="RRWFGHR"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007438">
        <property type="entry name" value="DUF488"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04343">
        <property type="entry name" value="DUF488"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="163" mass="18451" checksum="51BDE099D43D4DBE" modified="2005-10-11" version="1">MAFSSAACPQARGYRFASRKHVRSESGDRAFDPIKAAENLVTRLAARNISVKRAYEAPAADDGSRILVDRLWPRGLPKTEAAIDLWLKDIAPSTGLRKWFGHDPARWQEFRQRYVEEIHQHPEELDELRTRAKAGRVTLVFAAHDEAHNNAVVLRDVLLGRVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="129" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNV9</accession>
    <name>Q3SNV9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Signal recognition particle protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Fifty-four homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ffh</name>
        <name evidence="11" type="ordered locus">Nwi_2779</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY. Interaction with FtsY leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. SRP is a ribonucleoprotein composed of Ffh and a 4.5S RNA molecule.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="8">The SRP-RNC complex is targeted to the cytoplasmic membrane.</text>
    </comment>
    <comment type="domain">
        <text evidence="8">Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the GTP-binding SRP family. SRP54 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315977.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2779"/>
    <dbReference type="EnsemblBacteria" id="ABA06032">
        <property type="protein sequence ID" value="ABA06032"/>
        <property type="gene ID" value="Nwi_2779"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2779"/>
    <dbReference type="eggNOG" id="COG0541">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009301_6_0_5"/>
    <dbReference type="OMA" id="DTAGRHK"/>
    <dbReference type="OrthoDB" id="656414at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048500">
        <property type="term" value="C:signal recognition particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00306">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036891">
        <property type="entry name" value="Signal_recog_part_SRP54_M_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004125">
        <property type="entry name" value="Signal_recog_particle_SRP54_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004780">
        <property type="entry name" value="SRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022941">
        <property type="entry name" value="SRP54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564">
        <property type="entry name" value="PTHR11564"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564:SF7">
        <property type="entry name" value="PTHR11564:SF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02978">
        <property type="entry name" value="SRP_SPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47446">
        <property type="entry name" value="SSF47446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00959">
        <property type="entry name" value="ffh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 8" id="KW-0733">Signal recognition particle</keyword>
    <feature type="domain" description="SRP54" evidence="10">
        <location>
            <begin position="269"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="465"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="107"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="190"/>
            <end position="194"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="248"/>
            <end position="251"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA06032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="516" mass="55175" checksum="6F3F673A34D8441B" modified="2005-10-11" version="1">MFDNLSERLGGILDRLTRRGALTEADVDAAMREVRRALLEADVSLDVVRSFTDKVREQAIGATVVKSVTPGQMVVKIVHDELIATLGSDGQTIELNAVPPVIIMMVGLQGSGKTTTTAKLARRLTERDKRKVLMASLDIYRPAAMEQLAVLGRDLDIKTLPIVAGQKPEQIARRALEAGKLGGYDVVLLDTAGRTTLDEDMMSEAASIKTAAHPHEVLLVADSLTGQDAVNLARSFDERVGLTGIVLTRVDGDGRGGAALSMRQVTGKPIKLIGTGEKTDALEDFHPSRIAGRILGMGDVVSLVEKASANIDAEKAARTTERMRKGQFDLNDLREQLLQMSKMGGISGLMGMMPGVSKMKNQIAAAGLDDRIIKRQVAVIDSMTRQERKHPEILKASRKRRIAAGAGQKVEEINKLLKMHRSMADMMKMMGAGKRGPFAGIAQAMGFGGGMPSAEQMKALADKMPKGADGLPQLPKDLPAGLRGGLPNLPGLTGLTGKPTLPGLGKPGLGFPRKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNW3</accession>
    <name>Q3SNW3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">2-methylthioadenine synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_2775</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315973.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2775"/>
    <dbReference type="EnsemblBacteria" id="ABA06028">
        <property type="protein sequence ID" value="ABA06028"/>
        <property type="gene ID" value="Nwi_2775"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2775"/>
    <dbReference type="eggNOG" id="COG0621">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018697_1_1_5"/>
    <dbReference type="OMA" id="HFHIPLQ"/>
    <dbReference type="OrthoDB" id="397139at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035596">
        <property type="term" value="F:methylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005839">
        <property type="entry name" value="Methylthiotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013848">
        <property type="entry name" value="Methylthiotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038135">
        <property type="entry name" value="Methylthiotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006467">
        <property type="entry name" value="MiaB-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00919">
        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01579">
        <property type="entry name" value="MiaB-like-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00089">
        <property type="entry name" value="TIGR00089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MTTase N-terminal" evidence="8">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="9">
        <location>
            <begin position="139"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA06028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="422" mass="46054" checksum="AABBFD6798296C9D" modified="2005-10-11" version="1">MPVDVITFGCRLNAFESEVIRRNAEAAGLEDVVIVNSCAVTNEAVAQARQSIRRLKRARPDARIVVTGCAAQIEPAMFAQMAEVDRVIGNDEKMRGEAWRAARAAFAAPDVTIAAEKKIAVADIMAVTEMAPHLVDGFQGGLPRAFVQVQNGCDHRCTFCIIPFGRGNSRSMPMDAVVDQVRVLTETGYAEIVLTGVDLTSYGADLPGGPRLGGLVRQILRQVPEMRRLRISSIDSIEVDRDLMDAIANEERLMPHLHLSLQSGDDMILKRMKRRHARRDAIDFCAQVRRLRPDTAFGADIIAGFPTETDDMFARSLDLVAECDLTFLHVFPYSSRPGTPAARMPPVAAEVVRNRAGRLRAAAGAALRRRLAAEVGATRRVLIESGTQGRTEHFVPVAMTGDTPGAVRTVRVAGHDDTRLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP04</accession>
    <name>Q3SP04_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Transketolase</fullName>
            <ecNumber evidence="3 8">2.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_2734</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>D-glyceraldehyde 3-phosphate + D-sedoheptulose 7-phosphate = aldehydo-D-ribose 5-phosphate + D-xylulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:10508"/>
            <dbReference type="ChEBI" id="CHEBI:57483"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="2.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) ion per subunit. Can also utilize other divalent metal cations, such as Ca(2+), Mn(2+) and Co(2+).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
        <text evidence="8">Binds 1 thiamine pyrophosphate per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the transketolase family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.1" evidence="3 8"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315932.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2734"/>
    <dbReference type="EnsemblBacteria" id="ABA05987">
        <property type="protein sequence ID" value="ABA05987"/>
        <property type="gene ID" value="Nwi_2734"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2734"/>
    <dbReference type="eggNOG" id="COG0021">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009227_0_0_5"/>
    <dbReference type="OMA" id="HHTEGIE"/>
    <dbReference type="OrthoDB" id="188169at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004802">
        <property type="term" value="F:transketolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02012">
        <property type="entry name" value="TPP_TK"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="THDP-binding"/>
    </dbReference>
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        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005478">
        <property type="entry name" value="Transketolase_bac-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020826">
        <property type="entry name" value="Transketolase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033247">
        <property type="entry name" value="Transketolase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005474">
        <property type="entry name" value="Transketolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43522">
        <property type="entry name" value="PTHR43522"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00456">
        <property type="entry name" value="Transketolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00232">
        <property type="entry name" value="tktlase_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00801">
        <property type="entry name" value="TRANSKETOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00802">
        <property type="entry name" value="TRANSKETOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="6 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="4 8 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TRANSKETOLASE_1" evidence="9">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA05987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="661" mass="71369" checksum="D98EAD34B2852FAA" modified="2005-10-11" version="1">MPTVDSSRMANAIRALAMDGVEKAKSGHPGLPMGTADIATVLFTKFLKYDAADPHWPDRDRFILSAGHGSMLLYALLYLTGYKQMTLDQLKNFRQLDSKTPGHPENFITEGAETTTGPLGQGVATSVGFALAERLLAAEFGGEVVDHYTYVLCSDGDLMEGISHEAIALAGHLKLNKLIFLYDDNGITIDGPISLADDVDQVARFKACGWDARRIDGHDQKAIAAAIRRAQKSDRPSLIACKTIIGYGAPTRAGTSKAHGEALGAEELAGTKKALDWEYGPFEVPDDVLNAWRSAGRKGKRHNKEWRARLAAQPDAQRDEFKRRVIERKPSQNLNEAIRKLKDKLVAEPPTVATRKASEMALEALVPAVPELLLGSADLTPSNNTRTKGLKEVEPGQYGGRYIHYGIRELGMAAAMNGIALHGGYAPAGGTFMCFADYARPAMRVSALAYIPTIYIMTHDSIGLGEDGPTHQPVEHLASLRAMPNMRVFRPADAVETAECWQLALERPKGPTVLALSRQNLAPVRKTADTDNRCAHGAYELVAPNGEAKVSIFASGSEVEIAVAAQQQLSQRGIASRVVSVPSLELLLEQPEDRRQAIIGKAPVKIAVEAAVRFGWDAVIGADGLFVGMNSFGHSAPAKDLYKHFGITAEAVVSAVEKRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPD2</accession>
    <name>Q3SPD2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2606</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2606"/>
    <dbReference type="EnsemblBacteria" id="ABA05859">
        <property type="protein sequence ID" value="ABA05859"/>
        <property type="gene ID" value="Nwi_2606"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2606"/>
    <dbReference type="eggNOG" id="COG5389">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104595_1_0_5"/>
    <dbReference type="OMA" id="RPEKIAW"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007922">
        <property type="entry name" value="DUF721/UPF0232"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05258">
        <property type="entry name" value="DciA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="209" mass="22765" checksum="00446D9E48479855" modified="2005-10-11" version="1">MPGLVPGIHVFFVSCRKRRAKNGGWAGTRPAMTPSRQAHPNENPTCYSPPMSKPGPISAKPLSALLGRVFSDAYARQGFASRELVTRWAAIAGPEIAAHSEPIKIQWPRPVEGQQQEPATLVLRVEGPVALEIQHSSNVILQRVNRFFGWNAVGRLALRQAPLSRKTSRRSARPPDATDVARVAGTLTSIEDDDLRAALARLGASIKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPF0</accession>
    <name>Q3SPF0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2588</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315791.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2588"/>
    <dbReference type="EnsemblBacteria" id="ABA05841">
        <property type="protein sequence ID" value="ABA05841"/>
        <property type="gene ID" value="Nwi_2588"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2588"/>
    <dbReference type="eggNOG" id="ENOG50314TJ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_195268_0_0_5"/>
    <dbReference type="OMA" id="RWAYKTA"/>
    <dbReference type="OrthoDB" id="2094779at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="62" mass="7017" checksum="E624A8BD27E7ECB2" modified="2005-10-11" version="1">MPPLIALAATLGGLVAVRWLYNTARRINQELEEARMARVAEPARRDIPTLRRDSRTGAYRPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPM1</accession>
    <name>Q3SPM1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_2517</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315721.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2517"/>
    <dbReference type="EnsemblBacteria" id="ABA05770">
        <property type="protein sequence ID" value="ABA05770"/>
        <property type="gene ID" value="Nwi_2517"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2517"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_27_5"/>
    <dbReference type="OMA" id="SQTYASN"/>
    <dbReference type="OrthoDB" id="1031685at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="8">
        <location>
            <begin position="210"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="270"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="466" mass="51592" checksum="AA9C9F5A64C406C2" modified="2005-10-11" version="1">MTSITATLDAGLTLIRTALRRVLVANSRIGHAFNKWMPKGLYARALLIMIVPMVVLQSVVAFVFMERHWNTVTRRLSTAVVQDVAALIDVYKDYPQDKDRTTIRRIAQQRLGLVVDFLPPGDMPPPGPKPFFSLLDQSLSVQISRQIGKPFWIDTVGRSNLVEIRIQLDDAVMRVFAQRSAAYASNSEIFIFWMLGTSSILLFVAVLFLRNQIKPILRLADAAESFGKGREAPHFRPRGAREVRRAAQAFIEMKMRIERSIEQRTAMLAGVSHDLRTILTRFKLELALIGDSPDVDGMRKDVDEMHGMLEAYLAFARGDSGEQAQPTDMAAALEELRGDAERNGHAATAIFNGLPVATVKPAAFKRCLGNLVSNAARYAETIAIIGHRDHRWLTVMVDDDGPGIPQDMREEVFKPFVRLDSARNQDEGGSGLGLAIARDIARSHGGDITLGDSPMGGLRATVRVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPU4</accession>
    <name>Q3SPU4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2444</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05697">
        <property type="protein sequence ID" value="ABA05697"/>
        <property type="gene ID" value="Nwi_2444"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2444"/>
    <dbReference type="HOGENOM" id="CLU_178291_1_0_5"/>
    <dbReference type="OMA" id="NQRESAH"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021738">
        <property type="entry name" value="DUF3309"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11752">
        <property type="entry name" value="DUF3309"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="54" mass="5408" checksum="F61A057D52A75B74" modified="2005-10-11" version="1">MSIGTIILIILIIALLGGFSGVGGGPFYGTGYYGGGGLGLVIVILLILILMGRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ07</accession>
    <name>Q3SQ07_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">UDP-glucose 6-dehydrogenase</fullName>
            <ecNumber evidence="4 7">1.1.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_2380</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>H2O + 2 NAD(+) + UDP-alpha-D-glucose = 3 H(+) + 2 NADH + UDP-alpha-D-glucuronate</text>
            <dbReference type="Rhea" id="RHEA:23596"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="1.1.1.22"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nucleotide-sugar biosynthesis; UDP-alpha-D-glucuronate biosynthesis; UDP-alpha-D-glucuronate from UDP-alpha-D-glucose: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.22" evidence="4 7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315595.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2380"/>
    <dbReference type="EnsemblBacteria" id="ABA05634">
        <property type="protein sequence ID" value="ABA05634"/>
        <property type="gene ID" value="Nwi_2380"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2380"/>
    <dbReference type="eggNOG" id="COG1004">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023810_1_2_5"/>
    <dbReference type="OMA" id="CECLNLP"/>
    <dbReference type="OrthoDB" id="647136at2"/>
    <dbReference type="UniPathway" id="UPA00038">
        <property type="reaction ID" value="UER00491"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003979">
        <property type="term" value="F:UDP-glucose 6-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006065">
        <property type="term" value="P:UDP-glucuronate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017476">
        <property type="entry name" value="UDP-Glc/GDP-Man"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014027">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036220">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014026">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001732">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028357">
        <property type="entry name" value="UDPglc_DH_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00984">
        <property type="entry name" value="UDPG_MGDP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03720">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03721">
        <property type="entry name" value="UDPG_MGDP_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500134">
        <property type="entry name" value="UDPglc_DH_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000124">
        <property type="entry name" value="UDPglc_GDPman_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00984">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52413">
        <property type="entry name" value="SSF52413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03026">
        <property type="entry name" value="NDP-sugDHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 10" id="KW-0520">NAD</keyword>
    <keyword evidence="5 7 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDPG_MGDP_dh_C" evidence="11">
        <location>
            <begin position="317"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="152"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="252"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="263"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA05634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="436" mass="47075" checksum="1BD9608B7402F305" modified="2005-10-11" version="1">MRIAMIGTGYVGLVSGACFADFGHQVTCVDKDAAKIAALCRGEIPIFEPGLDALVATNVKASRLDFTTDLAGPVAEAEAVFIAVGTPSRRGDGHADLSYVYAAAREIAAAIGDFTVVVTKSTVPVGTGDEVERLIGETNPSANVVVASNPEFLREGAAIRDFKFPDRIVIGTSDERGRKVLGDIYRPLSLNQAPLMFTERRTAELIKYAANAFLATKITFINEIADLSEKVGADIQEVARGIGLDNRIGTKFLHAGPGYGGSCFPKDTRALVKIAEDHDAQLRIVESVVTVNDNRKRAMARKVAHALGDNLRNKTIAVLGLAFKPDTDDMREAPSIPLITGLCDMGAKVRAFDPASMKEALRELPDIDYCDDAYSCAEGADALVIVTEWVQFRALDLPRLKRIMNQPIVVDLRNVYRPAELEELGFTYTGIGRSQA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-06-16" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ43</accession>
    <name>MIAA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA dimethylallyltransferase</fullName>
            <ecNumber evidence="1">2.5.1.75</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Dimethylallyl diphosphate:tRNA dimethylallyltransferase</fullName>
            <shortName evidence="1">DMAPP:tRNA dimethylallyltransferase</shortName>
            <shortName evidence="1">DMATase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Isopentenyl-diphosphate:tRNA isopentenyltransferase</fullName>
            <shortName evidence="1">IPP transferase</shortName>
            <shortName evidence="1">IPPT</shortName>
            <shortName evidence="1">IPTase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">miaA</name>
        <name type="ordered locus">Nwi_2344</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosine(37) in tRNA + dimethylallyl diphosphate = diphosphate + N(6)-dimethylallyladenosine(37) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:26482"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10375"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74415"/>
            <dbReference type="EC" id="2.5.1.75"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IPP transferase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.75" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315562.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SQ43"/>
    <dbReference type="SMR" id="Q3SQ43"/>
    <dbReference type="STRING" id="323098.Nwi_2344"/>
    <dbReference type="EnsemblBacteria" id="ABA05598">
        <property type="protein sequence ID" value="ABA05598"/>
        <property type="gene ID" value="Nwi_2344"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2344"/>
    <dbReference type="eggNOG" id="COG0324">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032616_0_1_5"/>
    <dbReference type="OMA" id="YAKRQYT"/>
    <dbReference type="OrthoDB" id="1069591at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052381">
        <property type="term" value="F:tRNA dimethylallyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00185">
        <property type="entry name" value="IPP_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039657">
        <property type="entry name" value="Dimethylallyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018022">
        <property type="entry name" value="IPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088">
        <property type="entry name" value="PTHR11088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00174">
        <property type="entry name" value="miaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000377243" description="tRNA dimethylallyltransferase">
        <location>
            <begin position="1"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with substrate tRNA" evidence="1">
        <location>
            <begin position="45"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="20"/>
            <end position="27"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="22"/>
            <end position="27"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with substrate tRNA" evidence="1">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with substrate tRNA" evidence="1">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00185"/>
        </source>
    </evidence>
    <sequence length="314" mass="34480" checksum="F9448EE7D82663B3" modified="2005-10-11" version="1">MGGLNRDEGVEATDAVLIAGPTASGKSALALSLAEQTGGVVINTDSMQVYRNLRILTARPTPDEEARAPHRLYGHVDASVNYSAGRWVADATAVLNETLAKKRLPIFVGGSGLYFKALTRGLSAVPPIPPEVRNDVRARLERDGAEALHAVLARCDSETAQRLRPRDRSRVARALEVVEATGRSLTDWHRDGLPPLLPAERVRAVFLAPERDALYARIDARFGAMLAAEALEEVAALAERRLDPLLPAMKAHGVPALIRHLRGEISLERAAEIGRADTRHYAKRQFTWFRHQLPEFIWAAPAEARQVLERAEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQD7</accession>
    <name>Q3SQD7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2250</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2250"/>
    <dbReference type="EnsemblBacteria" id="ABA05504">
        <property type="protein sequence ID" value="ABA05504"/>
        <property type="gene ID" value="Nwi_2250"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2250"/>
    <dbReference type="eggNOG" id="COG5330">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056688_0_0_5"/>
    <dbReference type="OMA" id="LRFHRMQ"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019285">
        <property type="entry name" value="DUF2336"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10098">
        <property type="entry name" value="DUF2336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="268" mass="29326" checksum="8DE0E2F3FEA14D6D" modified="2005-10-11" version="1">MLSQSERLSDDALIENVRSKSQAHLLAISTRRVLSAAVTDALIERGNDAVVKSTVNNADAEFSEFGFTRLVERAEGDDDMATCIGMRPSIPRHHYLKLIAKASATVRARLAAAYPQQANDVSAVVGEVARRARSAPEAIGRETTIAHGLVRSLYEDGRLDEQEVASFAEARKFDETNAAIACLANVSISIAEKMMVESDTEGVLILAKVSGLSWTTVRTIIDMRRDLTQVEVTDIEAARQTYERLRQSTAQQVLRFHRTQQAVPAGDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQH9</accession>
    <name>Q3SQH9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2208</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315431.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05462">
        <property type="protein sequence ID" value="ABA05462"/>
        <property type="gene ID" value="Nwi_2208"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2208"/>
    <dbReference type="eggNOG" id="ENOG5030SSI">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1479389_0_0_5"/>
    <dbReference type="OMA" id="HALYDRP"/>
    <dbReference type="OrthoDB" id="1780793at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="97"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="174" mass="18781" checksum="8B128E9F42057DDC" modified="2005-10-11" version="1">MTSSWRISTVNGALLALYFIPVWTFVAFKIVASPVHGLYDLANVSVALFLSDHLHVVAMDLVRFAWLLALGKMTVVVFFAVFLVMTTRASVRKSGSFDEALALALGIGSSIAFASMVMASQVGEMAALRLHATELLLLLGGAILMATEPPFQRDPPVRPWPSQPNVQPFTSSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQJ0</accession>
    <name>Q3SQJ0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2197</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315420.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2197"/>
    <dbReference type="EnsemblBacteria" id="ABA05451">
        <property type="protein sequence ID" value="ABA05451"/>
        <property type="gene ID" value="Nwi_2197"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2197"/>
    <dbReference type="eggNOG" id="COG5483">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077467_2_0_5"/>
    <dbReference type="OMA" id="MRRIYCE"/>
    <dbReference type="OrthoDB" id="1831995at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007438">
        <property type="entry name" value="DUF488"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014519">
        <property type="entry name" value="UCP024492"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04343">
        <property type="entry name" value="DUF488"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF024492">
        <property type="entry name" value="UCP024492"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="153" mass="17205" checksum="0B35FA8703274102" modified="2005-10-11" version="1">MKSPTPLFTIGYEQTPANAVLDELQRAGIKWLVDVRAVTSSRRPGFSKNQLAASLGDRGIAYLHLRGLGTPKQGRLARRSGKFDLLHEIYAAHLKTPQAREEIDQLSALVRQSGPVCILCYERDHQHCHRRWIAETIETRNGVTVENLIARPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQP1</accession>
    <name>Q3SQP1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 14">Inosine-5'-monophosphate dehydrogenase</fullName>
            <shortName evidence="7">IMP dehydrogenase</shortName>
            <shortName evidence="7">IMPD</shortName>
            <shortName evidence="7">IMPDH</shortName>
            <ecNumber evidence="7 14">1.1.1.205</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">guaB</name>
        <name evidence="16" type="ordered locus">Nwi_2146</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 14">
            <text>H2O + IMP + NAD(+) = H(+) + NADH + XMP</text>
            <dbReference type="Rhea" id="RHEA:11708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="1.1.1.205"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="7">Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7 14">Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 13">Belongs to the IMPDH/GMPR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.205" evidence="7 14"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315369.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2146"/>
    <dbReference type="EnsemblBacteria" id="ABA05400">
        <property type="protein sequence ID" value="ABA05400"/>
        <property type="gene ID" value="Nwi_2146"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2146"/>
    <dbReference type="eggNOG" id="COG0516">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0517">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022552_2_1_5"/>
    <dbReference type="OMA" id="MGYCGAK"/>
    <dbReference type="OrthoDB" id="532857at2"/>
    <dbReference type="UniPathway" id="UPA00601">
        <property type="reaction ID" value="UER00295"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003938">
        <property type="term" value="F:IMP dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006177">
        <property type="term" value="P:GMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00381">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01964">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
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    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
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    <dbReference type="InterPro" id="IPR005990">
        <property type="entry name" value="IMP_DH"/>
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    <dbReference type="InterPro" id="IPR015875">
        <property type="entry name" value="IMP_DH/GMP_Rdtase_CS"/>
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    <dbReference type="InterPro" id="IPR001093">
        <property type="entry name" value="IMP_DH_GMPRt"/>
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    <dbReference type="PANTHER" id="PTHR11911">
        <property type="entry name" value="PTHR11911"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00478">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000130">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01302">
        <property type="entry name" value="IMP_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00487">
        <property type="entry name" value="IMP_DH_GMP_RED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0129">CBS domain</keyword>
    <keyword evidence="3 7 14" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="2 7 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10 14" id="KW-0520">NAD</keyword>
    <keyword evidence="5 7 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 7 11" id="KW-0630">Potassium</keyword>
    <keyword evidence="7 14" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="15">
        <location>
            <begin position="98"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="15">
        <location>
            <begin position="163"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="IMP binding" evidence="7 9">
        <location>
            <begin position="347"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="IMP binding" evidence="7 9">
        <location>
            <begin position="370"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="IMP binding" evidence="7 9">
        <location>
            <begin position="394"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="active site" description="Thioimidate intermediate" evidence="7 8">
        <location>
            <position position="314"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7 8">
        <location>
            <position position="410"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="257"/>
            <end position="259"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 10">
        <location>
            <begin position="307"/>
            <end position="309"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 11">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 11">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 11">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="425"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
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    <feature type="binding site" evidence="7">
        <location>
            <position position="479"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
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    <feature type="binding site" evidence="7">
        <location>
            <position position="480"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="481"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ABA05400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="498" mass="52367" checksum="E699E452844173B0" modified="2005-10-11" version="1">MALENGSRDIKEAYTFDDVLLKPGASDILPSEADVSSRVTRAIPLNIPIMASAMDTVTEARMAIAMAQAGGLGVIHRNFDVEGQAAQVRQVKKFESGMVVNPLTIGPDAMLSDALALMSDHGFSGIPVVAGGSGAAPGRLVGILTNRDVRFATDPRQKVSELMTHENLVTVREGVSQEEAKRMLHKHRIEKLLVVDDQYRCVGLITVKDMEKAVAHPLASKDSQGRLRVAAATTVGEGGFERTERLIDAGVDLIVVDTAHGHSSRVLEAVNRIKRLSNAVQVIAGNIATAEGAQALIDAGADAIKVGIGPGSICTTRIVAGVGVPQLTAIMDAAEAAKKAGVPVIADGGVKYSGDLAKALAAGADIVMVGSLLAGTDETPGEVYLWQGRSYKAYRGMGSVGAMSRGSADRYFQQDIKDTLKLVPEGIEGQVPYKGPVGNVMHQLAGGLRAAMGYVGARNLAEFHDKARFVRITGAGLRESHVHDVTITRESPNYPGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR14</accession>
    <name>Q3SR14_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GCN5-related N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2018</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315263.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2018"/>
    <dbReference type="EnsemblBacteria" id="ABA05277">
        <property type="protein sequence ID" value="ABA05277"/>
        <property type="gene ID" value="Nwi_2018"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2018"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099842_0_0_5"/>
    <dbReference type="OMA" id="TLYLHDM"/>
    <dbReference type="OrthoDB" id="2055010at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="10"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="177" mass="18880" checksum="6435835AD7A6665F" modified="2005-10-11" version="1">MPELSAEAKGQWRPMSSADLPRVMAIAGQVHPAFPEDGEIFAERLRLYAGGCLVCRAGSDIGGYVISHPWRALDPPALNSRLGELPGDPETYYLHDLALLPQLRATGAASLAVSQALARAGSEKLATVSLVAVNGSAGFWRRHGFRAIPLDAIGADSTLARKLRGYSKAAAFMVRQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR49</accession>
    <name>Q3SR49_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ethanolamine utilization protein EutN/carboxysome structural protein Ccml</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1983</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315229.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1983"/>
    <dbReference type="EnsemblBacteria" id="ABA05242">
        <property type="protein sequence ID" value="ABA05242"/>
        <property type="gene ID" value="Nwi_1983"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1983"/>
    <dbReference type="eggNOG" id="COG4576">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148498_1_0_5"/>
    <dbReference type="OMA" id="GCKPGDW"/>
    <dbReference type="OrthoDB" id="1832681at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031469">
        <property type="term" value="C:bacterial microcompartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01614">
        <property type="entry name" value="EutN_CcmL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014076">
        <property type="entry name" value="CsoS4A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004992">
        <property type="entry name" value="EutN_CcmL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036677">
        <property type="entry name" value="EutN_CcmL_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36539">
        <property type="entry name" value="PTHR36539"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03319">
        <property type="entry name" value="EutN_CcmL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159133">
        <property type="entry name" value="SSF159133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02703">
        <property type="entry name" value="carboxysome_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51932">
        <property type="entry name" value="BMV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="85" mass="9069" checksum="82FDEEAABEEB4A97" modified="2005-10-11" version="1">MKIMRVRTTLVSTNRIDAMGHRPLLVVQETPNGPRSVAVDAVGCIPGDWVICVGSSAARDAAGAKDYPSDLTIVGIIDHWNPEVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR56</accession>
    <name>Q3SR56_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ATPase associated with various cellular activities, AAA_5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1976</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CbbQ/NirQ/NorQ/GpvN family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1976"/>
    <dbReference type="EnsemblBacteria" id="ABA05235">
        <property type="protein sequence ID" value="ABA05235"/>
        <property type="gene ID" value="Nwi_1976"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1976"/>
    <dbReference type="eggNOG" id="COG0714">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067562_0_0_5"/>
    <dbReference type="OMA" id="DINGTKW"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013615">
        <property type="entry name" value="CbbQ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08406">
        <property type="entry name" value="CbbQ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_5" evidence="2">
        <location>
            <begin position="76"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="CbbQ_C" evidence="3">
        <location>
            <begin position="222"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="307" mass="33730" checksum="ABF74F6D633163B2" modified="2005-10-11" version="1">MRDDLTNRGYLPRRNPAFHVPAAASARSEAAPRAVTDTTGAASPLDAYRVAKEPYYRAVADEIALYEAAYSVRMPMMLKGPTGCGKTRFVEYMAWKLGKPLVTVACNEDMTASDLVGRFLLDANGTRWQDGPLTMAARFGGICYLDEVVEARQDTTVVIHSLTDARRVLPLEKKGEIVEAHPDFQVVISYNPGYQSLMKDLKQSTKQRFGALDFNYPEHEVEAEIVVHESGVGLDAAKKLVHIAEHARNLKGHGLDEGISTRMLIYAGSLISRGVAPLAACRVALVRPITDDPDMRNALDSAVTTYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR98</accession>
    <name>Q3SR98_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Porin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_1933</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Forms passive diffusion pores that allow small molecular weight hydrophilic materials across the outer membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell outer membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="4">Consists of 16-stranded beta-barrel sheets, with large surface-exposed loops, that form a transmembrane pore at the center of each barrel. The pore is partially ocluded by a peptide loop that folds into the pore lumen.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the alphaproteobacteria porin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315187.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1933"/>
    <dbReference type="EnsemblBacteria" id="ABA05193">
        <property type="protein sequence ID" value="ABA05193"/>
        <property type="gene ID" value="Nwi_1933"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1933"/>
    <dbReference type="eggNOG" id="COG3203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044836_2_0_5"/>
    <dbReference type="OMA" id="LAPINYA"/>
    <dbReference type="OrthoDB" id="837523at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003684">
        <property type="entry name" value="Porin_alphabac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02530">
        <property type="entry name" value="Porin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0626">Porin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="2 4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009368701" description="Porin" evidence="4">
        <location>
            <begin position="25"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="522" mass="54975" checksum="4AE95D7B5995DF79" modified="2005-10-11" version="1">MNNLMKGLFLGSAAGLVVISGAQAADLPLKAKAVEYVRVCSMYGAGFYYIPGTDTCIKLGGYARVDFGVNTNAPLFFGNTATQAGAQNRYSNAFTWRSRADLTIDTRTATDYGVVRTYFDGGFTWTSGGYGGVRVGGTAYDASPAGASGNGAVVGGQLGVYFSFIQFAGFTVGKAVSQFTAPWGNYPGMNISDALVGGGGAFSGVNQLTYTAEFGNGVSAAISAQDPSAYYQAGIFNLGVASPGRTPIWTGVPPFFGPVFLPPVMLGYGLSAYGGTTMPDFVGQVRVDQAWGLVQASVAAHHNNPAYYGGVETTGGPANKWGWAGQLALTIRNLPTGPGDVINISGVYTHGATRYNIQDMTAQAGGSTIYSGTDSIGFGFAPDSVYAPNGDLQLVTTWGMRGGYVHNWSPHWSSSIFGAYASIRYTDGAKAYMCGVSIPFALLGGLTTCNPDYNIAQVGANVRWTPVKNLTFTTEAAYSMLDQKFAGVANVPNNWAVGKPEGNYTVKDQDMWSVMFRAQRTW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRA4</accession>
    <name>SSRP_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SsrA-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Small protein B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">smpB</name>
        <name type="ordered locus">Nwi_1927</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for rescue of stalled ribosomes mediated by trans-translation. Binds to transfer-messenger RNA (tmRNA), required for stable association of tmRNA with ribosomes. tmRNA and SmpB together mimic tRNA shape, replacing the anticodon stem-loop with SmpB. tmRNA is encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and it encodes a 'tag peptide', a short internal open reading frame. During trans-translation Ala-aminoacylated tmRNA acts like a tRNA, entering the A-site of stalled ribosomes, displacing the stalled mRNA. The ribosome then switches to translate the ORF on the tmRNA; the nascent peptide is terminated with the 'tag peptide' encoded by the tmRNA and targeted for degradation. The ribosome is freed to recommence translation, which seems to be the essential function of trans-translation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">The tmRNA-SmpB complex associates with stalled 70S ribosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SmpB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315183.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRA4"/>
    <dbReference type="SMR" id="Q3SRA4"/>
    <dbReference type="STRING" id="323098.Nwi_1927"/>
    <dbReference type="EnsemblBacteria" id="ABA05187">
        <property type="protein sequence ID" value="ABA05187"/>
        <property type="gene ID" value="Nwi_1927"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1927"/>
    <dbReference type="eggNOG" id="COG0691">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108953_0_1_5"/>
    <dbReference type="OMA" id="WTNHSAR"/>
    <dbReference type="OrthoDB" id="1720952at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070929">
        <property type="term" value="P:trans-translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09294">
        <property type="entry name" value="SmpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00023">
        <property type="entry name" value="SmpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023620">
        <property type="entry name" value="SmpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000037">
        <property type="entry name" value="SsrA-bd_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020081">
        <property type="entry name" value="SsrA-bd_prot_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30308">
        <property type="entry name" value="PTHR30308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01668">
        <property type="entry name" value="SmpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74982">
        <property type="entry name" value="SSF74982"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00086">
        <property type="entry name" value="smpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01317">
        <property type="entry name" value="SSRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <feature type="chain" id="PRO_1000002094" description="SsrA-binding protein">
        <location>
            <begin position="1"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="133"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="157" mass="18008" checksum="79E5B4C6F6600070" modified="2005-10-11" version="1">MADKGERAIKVVAENRKARFNYAIEDTVEAGIALTGTEVKSIRNGKTTIAESYADSKNGEIWLINSNIPEYLQANRFNHEPKRPRKLLLHRKQINKLMGAVDREGMTLIPLKLYFNERGRAKLLLAIAKGKKLHDKRESEKKRDWGREKGRLLRARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRC8</accession>
    <name>Q3SRC8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nitrogen regulatory protein P-II</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_1903</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">In nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP. P-II-UMP allows the deadenylation of glutamine synthetase (GS), thus activating the enzyme. Conversely, in nitrogen excess P-II is deuridylated and promotes the adenylation of GS. P-II indirectly controls the transcription of the GS gene (glnA). P-II prevents NR-II-catalyzed conversion of NR-I to NR-I-phosphate, the transcriptional activator of glnA. When P-II is uridylylated to P-II-UMP, these events are reversed.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the P(II) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315159.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1903"/>
    <dbReference type="EnsemblBacteria" id="ABA05163">
        <property type="protein sequence ID" value="ABA05163"/>
        <property type="gene ID" value="Nwi_1903"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1903"/>
    <dbReference type="eggNOG" id="COG0347">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082268_0_0_5"/>
    <dbReference type="OMA" id="YRGTEHV"/>
    <dbReference type="OrthoDB" id="2021322at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002187">
        <property type="entry name" value="N-reg_PII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017918">
        <property type="entry name" value="N-reg_PII_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002332">
        <property type="entry name" value="N-reg_PII_urydylation_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30115">
        <property type="entry name" value="PTHR30115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00543">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039144">
        <property type="entry name" value="GlnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00340">
        <property type="entry name" value="PIIGLNB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00938">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00638">
        <property type="entry name" value="PII_GLNB_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51343">
        <property type="entry name" value="PII_GLNB_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00496">
        <property type="entry name" value="PII_GLNB_UMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="O-UMP-tyrosine" evidence="5">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR039144-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602187-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA05163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="112" mass="12347" checksum="DFEB8E5673C2BA96" modified="2005-10-11" version="1">MKKIEAIIKPFKLDEVKEALQEIGLQGITVIEAKGFGRQKGHAELYRGAEYVVDFLPKVKIEIVIGDDLVEKAIEAIRRAAQTGRIGDGKIFVSNIEEAIRIRTGESGLDAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRE1</accession>
    <name>Q3SRE1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315146.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05150">
        <property type="protein sequence ID" value="ABA05150"/>
        <property type="gene ID" value="Nwi_1890"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1890"/>
    <dbReference type="HOGENOM" id="CLU_2845394_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="65" mass="7131" checksum="2D258AA1549C6D17" modified="2005-10-11" version="1">MLDLSFVPTSSLPLLLISAFILMGVAWRRGVTAFKQKYPVYWVLAGGVAFAIAVATAVEFWLVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRG7</accession>
    <name>Q3SRG7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Lipoprotein releasing system, transmembrane protein, LolC/E family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_1864</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ABC-4 integral membrane protein family. LolC/E subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315120.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1864"/>
    <dbReference type="EnsemblBacteria" id="ABA05124">
        <property type="protein sequence ID" value="ABA05124"/>
        <property type="gene ID" value="Nwi_1864"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1864"/>
    <dbReference type="eggNOG" id="COG4591">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_8_1_5"/>
    <dbReference type="OMA" id="GWWQQNG"/>
    <dbReference type="OrthoDB" id="768211at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042953">
        <property type="term" value="P:lipoprotein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011925">
        <property type="entry name" value="LolCE_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02212">
        <property type="entry name" value="lolCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="34"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="283"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="327"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="392"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="MacB_PCD" evidence="10">
        <location>
            <begin position="42"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="9">
        <location>
            <begin position="313"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA05124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="426" mass="46595" checksum="224950868160E780" modified="2005-10-11" version="1">MDETMNEPLRTKPFAAFEWMLSGRYLRARRKEGFISVIAGFSFLGIMLGVATLIIVMAVMNGFRTELLSKILGLNGHLLVQPLESPLTDWKEVAERVSQVKGIRLAAPVVDGQALGSSPYNAAGVFVRGIRADDLNNLTSIARNIKQGTLDGFDEGQGVAIGRRLADQLSLHAGDSVTLVAPRGAVTPMGTTPRIKPYKIAAVFEIGMSEYDSTFVFMPLAEAQAYFNRANDVTSIEVYTDNPDKIDDFRKSVTEAAGRPVFLVDWRQRNSTFFNALQVERNVMFLILTLIVLVAALNIVSGLIMLVKDKGSDIAILRTMGASQGAIMRIFLITGAAIGVVGTLTGFVVGLVVCLNIESIRAFLSWLTSTELFSPELYFLSKLPAEIDARETSAVVIMALTLSFLATLYPSWRAARLDPVEALRYE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRJ4</accession>
    <name>TRPD_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Anthranilate phosphoribosyltransferase</fullName>
            <ecNumber evidence="1">2.4.2.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trpD</name>
        <name type="ordered locus">Nwi_1837</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of the phosphoribosyl group of 5-phosphorylribose-1-pyrophosphate (PRPP) to anthranilate to yield N-(5'-phosphoribosyl)-anthranilate (PRA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>diphosphate + N-(5-phospho-beta-D-ribosyl)anthranilate = 5-phospho-alpha-D-ribose 1-diphosphate + anthranilate</text>
            <dbReference type="Rhea" id="RHEA:11768"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:18277"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium ions per monomer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the anthranilate phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.18" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315093.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRJ4"/>
    <dbReference type="SMR" id="Q3SRJ4"/>
    <dbReference type="STRING" id="323098.Nwi_1837"/>
    <dbReference type="EnsemblBacteria" id="ABA05097">
        <property type="protein sequence ID" value="ABA05097"/>
        <property type="gene ID" value="Nwi_1837"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1837"/>
    <dbReference type="eggNOG" id="COG0547">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034315_2_1_5"/>
    <dbReference type="OMA" id="GPMTNPA"/>
    <dbReference type="OrthoDB" id="1238435at2"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00041"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004048">
        <property type="term" value="F:anthranilate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1030.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00211">
        <property type="entry name" value="TrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005940">
        <property type="entry name" value="Anthranilate_Pribosyl_Tfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000312">
        <property type="entry name" value="Glycosyl_Trfase_fam3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017459">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036320">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035902">
        <property type="entry name" value="Nuc_phospho_transferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43285">
        <property type="entry name" value="PTHR43285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02885">
        <property type="entry name" value="Glycos_trans_3N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00591">
        <property type="entry name" value="Glycos_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47648">
        <property type="entry name" value="SSF47648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52418">
        <property type="entry name" value="SSF52418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01245">
        <property type="entry name" value="trpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="chain" id="PRO_0000227171" description="Anthranilate phosphoribosyltransferase">
        <location>
            <begin position="1"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="84"/>
            <end position="85"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="91"/>
            <end position="94"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="109"/>
            <end position="117"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00211"/>
        </source>
    </evidence>
    <sequence length="337" mass="34374" checksum="8EF577181B100842" modified="2005-10-11" version="1">MDDLKVLIGKVATGATLTREEAAAAFNSMMSGEATPSQMGGLLMALRVRGETVDEITGAVSAMRSMMLRVKAPVDAVDVVGTGGDGSGSVNVSTCASFIVAGAGVTVAKHGNRALSSRSGAADCLAALGIRIDLTPEQVGRCINEAGIGFMFAPAHHPAMKNVGPTRVELATRTIFNLLGPLSNPAGVRRQMVGVFSRQWVQPLAQVLKNHGSESAWVVHGSDGLDEITLAGPTFVAALEDGNIRTFEVTPEDAGLERADGDALKGGDAEANAASLRAVLEGKPGAFRDVALLNAAAALIVAGKAKTLKEGVALGAKSLDGGGALNKLKQLIAVSNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRN6</accession>
    <name>Q3SRN6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Cysteine synthase</fullName>
            <ecNumber evidence="5 11">2.5.1.47</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Nwi_1794</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>hydrogen sulfide + O-acetyl-L-serine = acetate + L-cysteine</text>
            <dbReference type="Rhea" id="RHEA:14829"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:58340"/>
            <dbReference type="EC" id="2.5.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.47" evidence="5 11"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315051.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1794"/>
    <dbReference type="EnsemblBacteria" id="ABA05055">
        <property type="protein sequence ID" value="ABA05055"/>
        <property type="gene ID" value="Nwi_1794"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1794"/>
    <dbReference type="eggNOG" id="COG0031">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021018_1_0_5"/>
    <dbReference type="OMA" id="FWDSGER"/>
    <dbReference type="OrthoDB" id="1033353at2"/>
    <dbReference type="UniPathway" id="UPA00136">
        <property type="reaction ID" value="UER00200"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004124">
        <property type="term" value="F:cysteine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005856">
        <property type="entry name" value="Cys_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005859">
        <property type="entry name" value="CysK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01139">
        <property type="entry name" value="cysK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01136">
        <property type="entry name" value="cysKM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8 11" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="7 9 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PALP" evidence="12">
        <location>
            <begin position="25"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="9">
        <location>
            <begin position="195"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="10">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR605856-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR605856-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA05055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="325" mass="34223" checksum="FE5BBFBB16F2933D" modified="2005-10-11" version="1">MDTKPNNDALHHAGRGRVFDSIVDTVGNTPIVRLRRLPQQHGVSATILAKLEYFNPATSVKDRIGAAMIQAMEEAGKINADTVLIEPTSGNTGIALAFVAASRGYRLKLVMPESVSTERCKMLAFLGAEIVLTPAAEGMKGSIAMAEELVRTTPHAVMPQQFKNLANPEVHRRTTAEEIWNDTGGNIDWVVAGVGTGGTITGIGQALKPRKPSLRIVAVEPEESAVLSGGRHSPHRIQGIGAGFVPEILDRSAIDEIVKVDSATALETARALARSEGIPGGISSGAAIAAALAIGRRPGSEGKTILAVVPSFSERYLSTALFEGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRP8</accession>
    <name>Q3SRP8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1782</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315039.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05043">
        <property type="protein sequence ID" value="ABA05043"/>
        <property type="gene ID" value="Nwi_1782"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1782"/>
    <dbReference type="eggNOG" id="ENOG5031748">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_199017_0_0_5"/>
    <dbReference type="OMA" id="AMAERIC"/>
    <dbReference type="OrthoDB" id="2010777at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="77" mass="8866" checksum="AFC842B20057B4BE" modified="2005-10-11" version="1">MSSNIEPLARAMAERICRSHLMNEAEIPDWVDRHWEIAAAMLESGAMDEMGEWQPGQDWRQGLEAYRERLAAKHDIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRS0</accession>
    <name>Q3SRS0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADH-ubiquinone oxidoreductase 17.2 kD subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1760</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1760"/>
    <dbReference type="EnsemblBacteria" id="ABA05021">
        <property type="protein sequence ID" value="ABA05021"/>
        <property type="gene ID" value="Nwi_1760"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1760"/>
    <dbReference type="eggNOG" id="COG3761">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110455_4_0_5"/>
    <dbReference type="OMA" id="WHGWIHH"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007763">
        <property type="entry name" value="NDUFA12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12910">
        <property type="entry name" value="PTHR12910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05071">
        <property type="entry name" value="NDUFA12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0830">Ubiquinone</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="163" mass="18683" checksum="ED5261A2E3071316" modified="2005-10-11" version="1">MSSNRNRMIIARALAGVRDTPCDGFPAMKQFFLKLFTWWNGQTFGTQLWTARFGELVGRDSEGNCYYRTRGGRIDPALGFERRWVIYNGYTEATHIPPEWHGWIHHVVDVPPTKEDYQPREWQKPHVPNMTGTPAAYRPSGSTLASGRRPKATGDYQPWTPGQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-10-02" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRX8</accession>
    <name>PANC_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pantothenate synthetase</fullName>
            <shortName evidence="1">PS</shortName>
            <ecNumber evidence="1">6.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Pantoate--beta-alanine ligase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Pantoate-activating enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">panC</name>
        <name type="ordered locus">Nwi_1702</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-pantoate + ATP + beta-alanine = (R)-pantothenate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10912"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
            <dbReference type="ChEBI" id="CHEBI:29032"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57966"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantothenate from (R)-pantoate and beta-alanine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">The reaction proceeds by a bi uni uni bi ping pong mechanism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the pantothenate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.1" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314961.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRX8"/>
    <dbReference type="SMR" id="Q3SRX8"/>
    <dbReference type="STRING" id="323098.Nwi_1702"/>
    <dbReference type="EnsemblBacteria" id="ABA04963">
        <property type="protein sequence ID" value="ABA04963"/>
        <property type="gene ID" value="Nwi_1702"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1702"/>
    <dbReference type="eggNOG" id="COG0414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047148_0_0_5"/>
    <dbReference type="OMA" id="CNHKLEP"/>
    <dbReference type="OrthoDB" id="1661843at2"/>
    <dbReference type="UniPathway" id="UPA00028">
        <property type="reaction ID" value="UER00005"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004592">
        <property type="term" value="F:pantoate-beta-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00560">
        <property type="entry name" value="PanC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00158">
        <property type="entry name" value="PanC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003721">
        <property type="entry name" value="Pantoate_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042176">
        <property type="entry name" value="Pantoate_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21299:SF1">
        <property type="entry name" value="PTHR21299:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02569">
        <property type="entry name" value="Pantoate_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00018">
        <property type="entry name" value="panC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0566">Pantothenate biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000305497" description="Pantothenate synthetase">
        <location>
            <begin position="1"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="41"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>(R)-pantoate</name>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>beta-alanine</name>
            <dbReference type="ChEBI" id="CHEBI:57966"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="152"/>
            <end position="155"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>(R)-pantoate</name>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="189"/>
            <end position="192"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00158"/>
        </source>
    </evidence>
    <sequence length="283" mass="31340" checksum="E348C68912DDF1E4" modified="2005-10-11" version="1">MPPAPAIVRTLPSLRRALERLRARRGTIALVPTMGALHEGHLALVRQAKRRASRVIVSIFVNPTQFAPHEDFGSYPRTWKADLAKLAEAKTDLIWRPDVSTMYPRDFATRISTEGPAIARLEDHFRPHFFGGVTTVVGKLFIQCRPDVALFGQKDYQQLKVVTRMVADLDLGVKIVGVPIVREPDGLAMSSRNVYLSNEQRARAPTLYRTLKDAAGRLRNGDHLEAVMADGARTIEDTGFSLDYFEARHAETLSPVGSLKDGPVRLLVAAKIGDTRLIDNLGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS46</accession>
    <name>Q3SS46_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1634</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314894.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04895">
        <property type="protein sequence ID" value="ABA04895"/>
        <property type="gene ID" value="Nwi_1634"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1634"/>
    <dbReference type="eggNOG" id="ENOG5032NY8">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1871436_0_0_5"/>
    <dbReference type="OrthoDB" id="1941269at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229160" evidence="1">
        <location>
            <begin position="26"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="141" mass="15297" checksum="0DF364280D221B0A" modified="2005-10-11" version="1">MKASLVALLLALLLALLSHLAPAFAETPKSTDAPPVVQQEFDGFIKKFRTALKADDSAAVASMTQLPFMADASISDASAFRAKIYSGDFTARNRKCIQRGKAVYARDGANKDSYFIFCGDLIFTFTRTAQGFLLSEIGMND</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS71</accession>
    <name>Q3SS71_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cold-shock DNA-binding protein family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1609</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1609"/>
    <dbReference type="EnsemblBacteria" id="ABA04870">
        <property type="protein sequence ID" value="ABA04870"/>
        <property type="gene ID" value="Nwi_1609"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1609"/>
    <dbReference type="eggNOG" id="COG1278">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097141_2_0_5"/>
    <dbReference type="OMA" id="IKWFDAS"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00050">
        <property type="entry name" value="COLDSHOCK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CSD" evidence="2">
        <location>
            <begin position="50"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="CSD" evidence="2">
        <location>
            <begin position="142"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="217" mass="23523" checksum="0CC48D3B31B094A4" modified="2005-10-11" version="1">MGSDGFESRKFAGPAVSDPLGSKEFSGTNDREAGAGPFAGLGESPANIVEISGVIKWFDASKGYGFIVPDNGWPDVLLHVTVLRRDGYQTAYEGARLVCDCVQRAKGYQAFRITSMDESTAIHPAQMLPPRTHVSVTPTSGLERAQVKWFNRLRGFGFLTRGEGTPDIFVHMETLRRFGITELRPGQFVLVRFGPGSKGMMAAEVQPETGAPSVSSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="135" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSB2</accession>
    <name>Q3SSB2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">UvrABC system protein A</fullName>
            <shortName evidence="13">UvrA protein</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Excinuclease ABC subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">uvrA</name>
        <name evidence="17" type="ordered locus">Nwi_1568</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by UvrB, the UvrA molecules dissociate.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Forms a heterotetramer with UvrB during the search for lesions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the ABC transporter superfamily. UvrA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314835.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1568"/>
    <dbReference type="EnsemblBacteria" id="ABA04829">
        <property type="protein sequence ID" value="ABA04829"/>
        <property type="gene ID" value="Nwi_1568"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1568"/>
    <dbReference type="eggNOG" id="COG0178">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001370_0_2_5"/>
    <dbReference type="OMA" id="PFEGIIP"/>
    <dbReference type="OrthoDB" id="152379at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009380">
        <property type="term" value="C:excinuclease repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009381">
        <property type="term" value="F:excinuclease ABC activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00205">
        <property type="entry name" value="UvrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004602">
        <property type="entry name" value="UvrA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041552">
        <property type="entry name" value="UvrA_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041102">
        <property type="entry name" value="UvrA_inter"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17755">
        <property type="entry name" value="UvrA_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17760">
        <property type="entry name" value="UvrA_inter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00630">
        <property type="entry name" value="uvra"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 13" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 13" id="KW-0228">DNA excision</keyword>
    <keyword evidence="12 13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 13" id="KW-0267">Excision nuclease</keyword>
    <keyword evidence="2 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0742">SOS response</keyword>
    <keyword evidence="8 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ABC transporter" evidence="16">
        <location>
            <begin position="640"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="13">
        <location>
            <begin position="775"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="971"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="46"/>
            <end position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 14">
        <location>
            <begin position="676"/>
            <end position="683"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ABA04829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="993" mass="109497" checksum="D46A7E2BBC181DF7" modified="2005-10-11" version="1">MDEVIKAKRQQSSPASRAITIRGAREHNLKNVDLEIPRDKLVVFTGLSGSGKSSLAFDTIYAEGQRRYVESLSAYARQFLEMMQKPDVDQIDGLSPAISIEQKTTSKNPRSTVGTVTEIYDYMRLLWARVGVPYSPATGLPIESQTVSQMVDRVLALPDGTRLYLLAPVVRGRKGEYRKELAEYLKKGFQRVKIDGSFHELADAPALDKKFPHDIDVVVDRIVVRPDLGQRLAESFETALKLAEGLAVIEYADAPPPLPHGAGDKKKVAKIHDRSGPERILFSEKFACPVSGFTLPEIEPRLFSFNNPYGACPKCGGLGVEQHVDENLVIPNRELTLRKGAIAPWAKSSSPYYLQTLTALGKFYKFALDTRWKDLPKKTQAALLHGSGDNEIRFSYEDGVRSYETRKPFEGVIPNIERRYRETESEWAREELGKYFSDVPCDSCKGYRLKPEALCVKIGGKHIGEISELSVRGAGEWFETVPTMLSAQQNEIAGRVLKEIRERLSFLLDVGLGYLTLSRASGTLSGGESQRIRLASQIGSGLTGVLYVLDEPSIGLHQRDNARLLETLKRLRNLGNTVIVVEHDEDAIRLADHVLDIGPGAGVHGGHIVAQGTPADIMRNPRSLTGKYLTGELSVEIPERRPPNHRRTIKLVNARGNNLKNVTAEIPLGLFTCITGVSGGGKSTLLIDTLYKAIARKLNNASDPPAPHDRIEGLEHIDKIIDIDQSPIGRTPRSNPATYTGAFTPIREWFAGLPEAKARGYAPGRFSFNVKGGRCEACQGDGVIKIEMHFLPDVYVTCDACKGKRYNRETLEVLFKGKSISDVLDMTVEEAAEFFKAVPRVRDTFLTLQRVGLGYIRVGQQATTLSGGEAQRVKLAKELSKRATGRTLYILDEPTTGLHFHDVKKLLEVLHELVAQGNTVAVIEHNLEVIKTADWVIDLGPDGGDGGGEIVAWGAPEDIVKAPRSHTGKFLKPILEKAGKPRRHNPNASEAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSJ1</accession>
    <name>Q3SSJ1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1489</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04750">
        <property type="protein sequence ID" value="ABA04750"/>
        <property type="gene ID" value="Nwi_1489"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1489"/>
    <dbReference type="eggNOG" id="ENOG5032CJY">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1658945_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="32"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="159" mass="17297" checksum="ACF676C1E490B66F" modified="2005-10-11" version="1">MAQAESVLSTPPTNTSAQTIIELPQRAAGERLRQLAAQQRRAEKTRQRVRELREMASAEIERLIAFLDASDGYTMHELEAAVDDIPCDDNELDGAGDVDGEPSLGSANPHEVSAGVDQRLWAVGAADDREQDAAESGIADFEGLLEQIGQRDWQHTVMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSL4</accession>
    <name>Q3SSL4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endolytic peptidoglycan transglycosylase RlpA</fullName>
            <ecNumber evidence="3">4.2.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rlpA</name>
        <name evidence="7" type="ordered locus">Nwi_1466</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Lytic transglycosylase with a strong preference for naked glycan strands that lack stem peptides.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the RlpA family.</text>
    </comment>
    <dbReference type="EC" id="4.2.2.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1466"/>
    <dbReference type="EnsemblBacteria" id="ABA04727">
        <property type="protein sequence ID" value="ABA04727"/>
        <property type="gene ID" value="Nwi_1466"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1466"/>
    <dbReference type="eggNOG" id="COG0797">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042923_1_1_5"/>
    <dbReference type="OMA" id="LANCAQN"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008932">
        <property type="term" value="F:lytic endotransglycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000270">
        <property type="term" value="P:peptidoglycan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02071">
        <property type="entry name" value="RlpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034718">
        <property type="entry name" value="RlpA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012997">
        <property type="entry name" value="RplA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="SSF50685"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00413">
        <property type="entry name" value="rlpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009992555" description="Endolytic peptidoglycan transglycosylase RlpA" evidence="3">
        <location>
            <begin position="27"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="DPBB_1" evidence="6">
        <location>
            <begin position="86"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="219"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="260"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="235"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="279" mass="29592" checksum="F8B8C201A3875FCE" modified="2005-10-11" version="1" precursor="true">MGKSGSDRIRLAARALAAAGACLMLANCGSSSKLSRIDPKYGVSSSPRVVASGDTVPKGGGTYRIGKPYTVAGRVYVPQENTKYKAEGLASWYGDAFHGRLTANGEVFDMASLSAAHPTLPLPCYVRVTNLGNSKSLVVRVNDRGPYHGNRLIDVSNKAAELLEFKRNGVAPVRVEYVGRAPLEGSDDRQLMATLRTGVPAPSPSTVRVASARPFVPELRPSIGETSASSRVSTHRQESDGRREVSYGEGDVTGRVHPVAAYAPADQSGPSEFSARGLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSQ0</accession>
    <name>Q3SSQ0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1430</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1430"/>
    <dbReference type="EnsemblBacteria" id="ABA04691">
        <property type="protein sequence ID" value="ABA04691"/>
        <property type="gene ID" value="Nwi_1430"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1430"/>
    <dbReference type="eggNOG" id="ENOG5032S6E">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165306_1_0_5"/>
    <dbReference type="OMA" id="LMNHEPT"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021955">
        <property type="entry name" value="DUF3572"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12096">
        <property type="entry name" value="DUF3572"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="102" mass="11024" checksum="C48FB3DFDC6C3ADF" modified="2005-10-11" version="1">MVEMTKRVHNPREAAEFVAIQALSFVAADAEVLGRFLAETGIGPETLRSSASDPHFLTAVLDFVLRDDATVKAFSESSGHHPTTIAAARQAFGDVFPERDLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSR1</accession>
    <name>Q3SSR1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_1419</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314689.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1419"/>
    <dbReference type="EnsemblBacteria" id="ABA04680">
        <property type="protein sequence ID" value="ABA04680"/>
        <property type="gene ID" value="Nwi_1419"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1419"/>
    <dbReference type="eggNOG" id="COG2223">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_14_0_5"/>
    <dbReference type="OMA" id="LVIHTID"/>
    <dbReference type="OrthoDB" id="1217595at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015112">
        <property type="term" value="F:nitrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042128">
        <property type="term" value="P:nitrate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17341">
        <property type="entry name" value="MFS_NRT2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044772">
        <property type="entry name" value="NO3_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23515">
        <property type="entry name" value="PTHR23515"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0534">Nitrate assimilation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="239"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="272"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="309"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="345"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="375"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="8"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA04680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="418" mass="46023" checksum="C75DE57A8F15C3E3" modified="2005-10-11" version="1">MARSGNLSVLLITTWAFTVCFAVWMMFGVIGIPIREQLGLGSTEFGLLTATPVLTGALFRLPLGIWTDRFGGRIIMLLLLVGCAVPVWLSAYATTLWQFLLLGLALGLVGASFSVGTPYVARFFPRERRGFAMGFFGAGTIGAALNMFVSPWLIEAYGWQMVPKVYAVVLLVTALLFWALSARDPGVGSQAPSMWRQLSVLRDPRVWKYCQYYSIVFGGFTALSIWMPQYFKTEYGFTIAQASLLAACFSLPGGAFRALGGWLSDRFGAHNVTWWVLWVAWVCLFLLSYPRTDLIVHTVRDPLSFSIALPSWFFTMLLFTLGIAFACGMASTFKYIGDDFPDSMGAVSGVVGMAGGFGGFLLPITFGVILDWLGFNSSCFMLLYGIIWVSLTLNYLTEVRQAPVMGEQVRSPAAEQSR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSU3</accession>
    <name>RS11_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsK</name>
        <name type="ordered locus">Nwi_1387</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine-Dalgarno cleft in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Interacts with proteins S7 and S18. Binds to IF-3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009797114.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSU3"/>
    <dbReference type="SMR" id="Q3SSU3"/>
    <dbReference type="STRING" id="323098.Nwi_1387"/>
    <dbReference type="EnsemblBacteria" id="ABA04648">
        <property type="protein sequence ID" value="ABA04648"/>
        <property type="gene ID" value="Nwi_1387"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1387"/>
    <dbReference type="eggNOG" id="COG0100">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072439_5_0_5"/>
    <dbReference type="OMA" id="KWGVAHI"/>
    <dbReference type="OrthoDB" id="1713391at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019981">
        <property type="entry name" value="Ribosomal_S11_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_S11_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="PTHR11759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002131">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03632">
        <property type="entry name" value="uS11_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000230410" description="30S ribosomal protein S11">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01310"/>
        </source>
    </evidence>
    <sequence length="130" mass="13981" checksum="07EF5E6B7EAC2F11" modified="2005-10-11" version="1">MGKEATTRVRRRERKNIASGIAHVNSSFNNTTITITDAQGNTIAWSSAGTMGFKGSRKSTPYAAQVAAEDVSKKAQEHGMRTLEVEVAGPGSGRESALRALQAAGFTVTSIRDVTAIPHNGCRPRKRRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST09</accession>
    <name>Q3ST09_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1321</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314600.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1321"/>
    <dbReference type="EnsemblBacteria" id="ABA04582">
        <property type="protein sequence ID" value="ABA04582"/>
        <property type="gene ID" value="Nwi_1321"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1321"/>
    <dbReference type="eggNOG" id="COG0517">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040681_3_2_5"/>
    <dbReference type="OMA" id="APIKVYM"/>
    <dbReference type="OrthoDB" id="2014094at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd04623">
        <property type="entry name" value="CBS_pair_bac_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044725">
        <property type="entry name" value="CBSX3_CBS_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="10"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="75"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="142" mass="15425" checksum="2BD61107A8C9FCD0" modified="2005-10-11" version="1">MTVRAILSAKGPQVASVEPDVKLSAALRILSERQIGSVIVVSGARIEGILSERDVVRALDEHGAAALDQPVSAAMTRKVVSCRLSDTVAHLMEVMTAERFRHLPVVEEGKLVGLVSIGDVVKLRLQHYEAEQEALRDYINMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST18</accession>
    <name>Q3ST18_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutaredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_1312</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the glutaredoxin family. Monothiol subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314593.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1312"/>
    <dbReference type="EnsemblBacteria" id="ABA04573">
        <property type="protein sequence ID" value="ABA04573"/>
        <property type="gene ID" value="Nwi_1312"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1312"/>
    <dbReference type="eggNOG" id="COG0278">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026126_2_1_5"/>
    <dbReference type="OMA" id="KGTKLMP"/>
    <dbReference type="OrthoDB" id="2001991at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097573">
        <property type="term" value="F:glutathione oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03028">
        <property type="entry name" value="GRX_PICOT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033658">
        <property type="entry name" value="GRX_PICOT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014434">
        <property type="entry name" value="Monothiol_GRX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004480">
        <property type="entry name" value="Monothiol_GRX-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293">
        <property type="entry name" value="PTHR10293"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005894">
        <property type="entry name" value="Monothiol_GRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00365">
        <property type="entry name" value="TIGR00365"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4 7" id="KW-0408">Iron</keyword>
    <keyword evidence="5 7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="8">
        <location>
            <begin position="16"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR005894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR005894-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA04573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="112" mass="12314" checksum="B1EF9D3DD57DBA40" modified="2005-10-11" version="1">MSIKETIENELKSNDVVLFMKGTPQFPQCGFSGQVVQILDHVGVGYKGLNVLESPEMRDGIKTYSSWPTIPQLYVKGEFIGGCDIVREMFQAGELQKLLSDKGIAFNTPASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST48</accession>
    <name>Q3ST48_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase, IS4 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0973</name>
        <name evidence="3" type="ordered locus">Nwi_1282</name>
        <name evidence="4" type="ordered locus">Nwi_1592</name>
        <name evidence="5" type="ordered locus">Nwi_2473</name>
        <name evidence="6" type="ordered locus">Nwi_2498</name>
        <name evidence="7" type="ordered locus">Nwi_2824</name>
        <name evidence="8" type="ordered locus">Nwi_2861</name>
        <name evidence="9" type="ordered locus">Nwi_2923</name>
        <name evidence="10" type="ordered locus">Nwi_3013</name>
        <name evidence="11" type="ordered locus">Nwi_3037</name>
    </gene>
    <organism evidence="3 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="3 12" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="3">Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04235">
        <property type="protein sequence ID" value="ABA04235"/>
        <property type="gene ID" value="Nwi_0973"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04543">
        <property type="protein sequence ID" value="ABA04543"/>
        <property type="gene ID" value="Nwi_1282"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04853">
        <property type="protein sequence ID" value="ABA04853"/>
        <property type="gene ID" value="Nwi_1592"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05726">
        <property type="protein sequence ID" value="ABA05726"/>
        <property type="gene ID" value="Nwi_2473"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05751">
        <property type="protein sequence ID" value="ABA05751"/>
        <property type="gene ID" value="Nwi_2498"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06077">
        <property type="protein sequence ID" value="ABA06077"/>
        <property type="gene ID" value="Nwi_2824"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06111">
        <property type="protein sequence ID" value="ABA06111"/>
        <property type="gene ID" value="Nwi_2861"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06173">
        <property type="protein sequence ID" value="ABA06173"/>
        <property type="gene ID" value="Nwi_2923"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06263">
        <property type="protein sequence ID" value="ABA06263"/>
        <property type="gene ID" value="Nwi_3013"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06287">
        <property type="protein sequence ID" value="ABA06287"/>
        <property type="gene ID" value="Nwi_3037"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0973"/>
    <dbReference type="KEGG" id="nwi:Nwi_1282"/>
    <dbReference type="KEGG" id="nwi:Nwi_1592"/>
    <dbReference type="KEGG" id="nwi:Nwi_2473"/>
    <dbReference type="KEGG" id="nwi:Nwi_2498"/>
    <dbReference type="KEGG" id="nwi:Nwi_2824"/>
    <dbReference type="KEGG" id="nwi:Nwi_2861"/>
    <dbReference type="KEGG" id="nwi:Nwi_2923"/>
    <dbReference type="KEGG" id="nwi:Nwi_3013"/>
    <dbReference type="KEGG" id="nwi:Nwi_3037"/>
    <dbReference type="eggNOG" id="COG3385">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055261_9_1_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE_Tnp_1" evidence="1">
        <location>
            <begin position="4"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA06077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA06111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA06173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA06263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA06287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="122" mass="13590" checksum="92DBDAC02B7F0040" modified="2005-10-11" version="1">MLADALGRPLRFIVTAGQVGDITQAPALLEAQAGDAVLADKAYDSNALRALIAGMGAEAVIPSNRTRKIIIPHDAGLYKHRNRIERCFNRLKHFRRFATRYDRRTVHFTGFVHLAAALIWLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST52</accession>
    <name>Q3ST52_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ISSpo3, transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1278</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314567.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04539">
        <property type="protein sequence ID" value="ABA04539"/>
        <property type="gene ID" value="Nwi_1278"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1278"/>
    <dbReference type="eggNOG" id="COG3677">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044348_1_2_5"/>
    <dbReference type="OMA" id="KLMQVMT"/>
    <dbReference type="OrthoDB" id="1667004at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024445">
        <property type="entry name" value="Tnp_ISXO2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024442">
        <property type="entry name" value="Transposase_Zn_ribbon"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12762">
        <property type="entry name" value="DDE_Tnp_IS1595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12760">
        <property type="entry name" value="Zn_Tnp_IS1595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01126">
        <property type="entry name" value="DDE_Tnp_IS1595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE_Tnp_IS1595" evidence="1">
        <location>
            <begin position="130"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="292" mass="34158" checksum="C5F33497E535BCA9" modified="2005-10-11" version="1">MAQKPSKIREFQKRFPDEEACLTHLMRTRFGERLTCFKCQKQATYYRVKNRRSFECEHCGYQVYPTAGTPFEKTRTSLRDWFYVMFLFCETRNGVAAKEVQRQLGVTYKTAWRMCHLIRLYMGYVDGDYPMGGPGKTVEADETFIGGKDKAGKDDKAIVMGMIERGGDVVTRVVHARTKAHILPHIVRFVKEGSRVHTDEASNFKLLKEKYGYDHEAVDHSAKEYVRGDVHTNSIESFWSQVKRGINGTYVWVSKKHLQLYLREFEYRHNLRKQPGLMFELLLQAFPKVRVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST54</accession>
    <name>Q3ST54_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1276</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04537">
        <property type="protein sequence ID" value="ABA04537"/>
        <property type="gene ID" value="Nwi_1276"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1276"/>
    <dbReference type="eggNOG" id="ENOG5032MYK">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1804164_0_0_5"/>
    <dbReference type="OMA" id="PWNAMLT"/>
    <dbReference type="OrthoDB" id="2022285at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="138" mass="14878" checksum="0BA9AE54531FD662" modified="2005-10-11" version="1">MALIQNVLGTRPHARPPEPELPDDHQHGSPESPGAEISPQPEAEGSAEETGAPSPDQPESYDRRKTADELAGIILKALQTLDGAPAHGFVVTVYGGNPWNAMLTIKPEAGPIADPGLWRERVRDMAARLRQDFDLAET</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST69</accession>
    <name>Q3ST69_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF5659 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1261</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314550.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1261"/>
    <dbReference type="EnsemblBacteria" id="ABA04522">
        <property type="protein sequence ID" value="ABA04522"/>
        <property type="gene ID" value="Nwi_1261"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1261"/>
    <dbReference type="HOGENOM" id="CLU_2465912_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043718">
        <property type="entry name" value="DUF5659"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18903">
        <property type="entry name" value="DUF5659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5659" evidence="1">
        <location>
            <begin position="15"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="88" mass="9946" checksum="9AC4D04322F9CED6" modified="2005-10-11" version="1">MSSGQAKCDASVQDNGFETRDFYLACFLRCTGYELLDLRAEGRRKVFVFRDRPSRRDDVLSFYGDGAMVPPLAFSTTVKDMKALLHNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST82</accession>
    <name>Q3ST82_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1247</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314537.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1247"/>
    <dbReference type="EnsemblBacteria" id="ABA04509">
        <property type="protein sequence ID" value="ABA04509"/>
        <property type="gene ID" value="Nwi_1247"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1247"/>
    <dbReference type="eggNOG" id="ENOG5030X8R">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147208_0_0_5"/>
    <dbReference type="OMA" id="RHRMLTN"/>
    <dbReference type="OrthoDB" id="1969011at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="113" mass="12573" checksum="5B0FC3563F668448" modified="2005-10-11" version="1">MRKQRPKETEEEHVVLQFRPRERAEPPKQPSSENAADNPSPDSAADPPPPPPPREEPDDFRHRMLTNAAAIAFTIALIAVGLWLARNIIDLRQIQDCALMGLRGCAHISTPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STB8</accession>
    <name>Q3STB8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1211</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314502.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="MEROPS" id="A32.001"/>
    <dbReference type="EnsemblBacteria" id="ABA04473">
        <property type="protein sequence ID" value="ABA04473"/>
        <property type="gene ID" value="Nwi_1211"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1211"/>
    <dbReference type="eggNOG" id="COG3577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099411_3_2_5"/>
    <dbReference type="OMA" id="VVPRGQM"/>
    <dbReference type="OrthoDB" id="1803947at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd05483">
        <property type="entry name" value="retropepsin_like_bacteria"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011969">
        <property type="entry name" value="Clan_AA_Asp_peptidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034122">
        <property type="entry name" value="Retropepsin-like_bacterial"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="SSF50630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02281">
        <property type="entry name" value="clan_AA_DTGA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229227" evidence="1">
        <location>
            <begin position="21"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="170" mass="18047" checksum="E4323D6BA5ECC613" modified="2005-10-11" version="1">MIRILLVIGMLAATAGAVVAYGDPARISAAGNLVSTMLREAAAGPPRSVQIPRTQSGDFTLHAKINGVKTPMIIDTGATSVVLTYETAKAAGLPLELLKYDVDVETAGGRARAARLTLDKLAVGKLVERSVPALVVPRGQMKANLLGMSFLNRLESWEVRADRLMLHGYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STP2</accession>
    <name>Q3STP2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hpt protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1087</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1087"/>
    <dbReference type="EnsemblBacteria" id="ABA04349">
        <property type="protein sequence ID" value="ABA04349"/>
        <property type="gene ID" value="Nwi_1087"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1087"/>
    <dbReference type="eggNOG" id="COG2198">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155085_0_1_5"/>
    <dbReference type="OMA" id="QMFARQA"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036641">
        <property type="entry name" value="HPT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008207">
        <property type="entry name" value="Sig_transdc_His_kin_Hpt_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01627">
        <property type="entry name" value="Hpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47226">
        <property type="entry name" value="SSF47226"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HPt" evidence="1">
        <location>
            <begin position="34"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="115" mass="11948" checksum="E86CC7E316A55BCB" modified="2005-10-11" version="1">MPSPPLVPENGPIDFDHLHRMTLGDDGLEREVLATFAGQAAELVVSLARLPPDAAMLAHKLKGSARAIGAIHVAAAAADLEIALRSGADPAEPRAMLNDAVGQACQAIRAILLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STS2</accession>
    <name>Q3STS2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Cell division protein FtsA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ftsA</name>
        <name evidence="8" type="ordered locus">Nwi_1057</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Cell division protein that is involved in the assembly of the Z ring. May serve as a membrane anchor for the Z ring.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Self-interacts. Interacts with FtsZ.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell inner membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="5">Localizes to the Z ring in an FtsZ-dependent manner. Targeted to the membrane through a conserved C-terminal amphipathic helix.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the FtsA/MreB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314353.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1057"/>
    <dbReference type="EnsemblBacteria" id="ABA04319">
        <property type="protein sequence ID" value="ABA04319"/>
        <property type="gene ID" value="Nwi_1057"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1057"/>
    <dbReference type="eggNOG" id="COG0849">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037850_3_0_5"/>
    <dbReference type="OMA" id="DGIIRHT"/>
    <dbReference type="OrthoDB" id="1044637at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02033">
        <property type="entry name" value="FtsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020823">
        <property type="entry name" value="Cell_div_FtsA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003494">
        <property type="entry name" value="SHS2_FtsA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02491">
        <property type="entry name" value="SHS2_FTSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003101">
        <property type="entry name" value="FtsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00842">
        <property type="entry name" value="FtsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01174">
        <property type="entry name" value="ftsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 5 6" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1 5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FtsA" evidence="7">
        <location>
            <begin position="23"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR003101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA04319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="440" mass="46696" checksum="2499421FA2C8DD0F" modified="2005-10-11" version="1">MTSLDCGQTPKMRPMQRNRSALVASLDIGTSKIACMIARLRPSAPNDALRGRTHAVELIGYSQIQSRGMKAGAVTDLAQCEQAVRQAVSLAEQMAKVRVESVLLPVSAGRLQGQLIEAASHIQGGAVTPSDISRVTSTGMHHATASGRTVLHALPAGYSLDGVKGIRDPRGMVARRFGVDMNVVTTEATVAKNLMLAVERCHLTVEAMAASPYVAGLSVLTDDEVDLGAAVVEMGAGTTTIAVYSAGRFIYASGFAIGGHHVTMDLARGLGACIADAERIKTLYGTVLTGGSDVRELMTVPAAGDSDCDAPQVVSRATIANIVRQRVEEIFEMVRDRLADSPFAAEPRARVVLSGGASQLTGVPELASRVLGRPVRIGRPLGFGRLPNEAKSASFSVPTGLLVYPQYAHLEHVEPRRTRQVRTGTDGYFGKVGRWLREGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STS9</accession>
    <name>Q3STS9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cell wall polymerase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Peptidoglycan polymerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_1050</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314346.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1050"/>
    <dbReference type="EnsemblBacteria" id="ABA04312">
        <property type="protein sequence ID" value="ABA04312"/>
        <property type="gene ID" value="Nwi_1050"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1050"/>
    <dbReference type="eggNOG" id="COG0772">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029243_1_1_5"/>
    <dbReference type="OMA" id="KLWWSNL"/>
    <dbReference type="OrthoDB" id="1133883at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008955">
        <property type="term" value="F:peptidoglycan glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013437">
        <property type="entry name" value="FtsW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001182">
        <property type="entry name" value="FtsW/RodA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30474">
        <property type="entry name" value="PTHR30474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01098">
        <property type="entry name" value="FTSW_RODA_SPOVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02614">
        <property type="entry name" value="ftsW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0133">Cell shape</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="59"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="176"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="265"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="303"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="340"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA04312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="383" mass="41509" checksum="BB8F91B9E27D573A" modified="2005-10-11" version="1">MISREQRTPLSEWWWTVDRLLLAAMVLLMLTGVVLSLAASPSVATRIGLDPFHFFHRHVMFLLPSIIVMVGVSFLSPRLIRRSALIVFAISIVLIVATLGLGPEVKGAKRWITILGVNIQASESAKPAFVVLAAWLFSESARKPEMPATSMALALLLSLVALLVMEPDFGQTMLILTVWGALFFIAGMRIIWVFGLAGTAMAGLFAAYMTVPHVAARIRRFMDPASGDTFQVDTAMEAFANGGWFGLGPGEGVAKRSLPDSHTDFVFAVGAEEFGIIMCLGLLTLFTFIVMRTLSRAYASEDLFSRFAASGLAIMFGMQAAINMAVNLHLIPAKGMTLPFISYGGSSMVSLAYGAGLMLALTRERPRTEMESIEESAVAARYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STT5</accession>
    <name>Q3STT5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1044</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314340.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1044"/>
    <dbReference type="EnsemblBacteria" id="ABA04306">
        <property type="protein sequence ID" value="ABA04306"/>
        <property type="gene ID" value="Nwi_1044"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1044"/>
    <dbReference type="eggNOG" id="COG5462">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144121_1_0_5"/>
    <dbReference type="OMA" id="KVKYDVQ"/>
    <dbReference type="OrthoDB" id="2059536at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="126" mass="14282" checksum="CB0DF629B644B139" modified="2005-10-11" version="1">MRIIHFLVVCALVYAASYVYRIKMDSTSRTERVSRLQAQVREQREAIAVLRAEWARLDAPQRLRILAERHLKLKPIEARQFDSLKNLPERPPSLVPPGTSDPIGAMIEIIDPDVITGSLPSQEAPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STU5</accession>
    <name>Q3STU5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1034</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1034"/>
    <dbReference type="EnsemblBacteria" id="ABA04296">
        <property type="protein sequence ID" value="ABA04296"/>
        <property type="gene ID" value="Nwi_1034"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1034"/>
    <dbReference type="eggNOG" id="COG2010">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3154675_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="54" mass="6220" checksum="F7FD98038CFEF2BD" modified="2005-10-11" version="1">MRIANSSLLVMRYPVEELEESLAEGTMTGHPSMPQFQFEADQINDFIKFLKSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STY3</accession>
    <name>Q3STY3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Proton-translocating NAD(P)(+) transhydrogenase</fullName>
            <ecNumber evidence="3">7.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_0996</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+)(in) + NAD(+) + NADPH = H(+)(out) + NADH + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:47992"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="7.1.1.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="7.1.1.1" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314297.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0996"/>
    <dbReference type="EnsemblBacteria" id="ABA04258">
        <property type="protein sequence ID" value="ABA04258"/>
        <property type="gene ID" value="Nwi_0996"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0996"/>
    <dbReference type="eggNOG" id="COG3288">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137885_2_0_5"/>
    <dbReference type="OMA" id="FVVTDRM"/>
    <dbReference type="OrthoDB" id="1933548at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024605">
        <property type="entry name" value="NADP_transhyd_a_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12769">
        <property type="entry name" value="PNTB_4TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="70"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="PNTB_4TM" evidence="11">
        <location>
            <begin position="15"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA04258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="105" mass="11357" checksum="B150EA301696444C" modified="2005-10-11" version="1">MEHVVQAVDPFVFRLSIFVLAVFVGYFVVWSVTPALHTPLMSVTNAISSVIVVGALLAVGVGMISEGSGWARAFGFVALVFACVNIFGGFLVTQRMLAMYKKKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU03</accession>
    <name>Q3SU03_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ISRm2011-2 transposase protein</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="4">Probable ISRm2011-2 transposase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0954</name>
        <name evidence="4" type="ordered locus">Nwi_0976</name>
        <name evidence="5" type="ordered locus">Nwi_1016</name>
        <name evidence="6" type="ordered locus">Nwi_1291</name>
        <name evidence="7" type="ordered locus">Nwi_1999</name>
        <name evidence="8" type="ordered locus">Nwi_2260</name>
        <name evidence="9" type="ordered locus">Nwi_2411</name>
        <name evidence="10" type="ordered locus">Nwi_2437</name>
        <name evidence="11" type="ordered locus">Nwi_2921</name>
    </gene>
    <organism evidence="4 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="4 12" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="4">Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04216">
        <property type="protein sequence ID" value="ABA04216"/>
        <property type="gene ID" value="Nwi_0954"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04238">
        <property type="protein sequence ID" value="ABA04238"/>
        <property type="gene ID" value="Nwi_0976"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04278">
        <property type="protein sequence ID" value="ABA04278"/>
        <property type="gene ID" value="Nwi_1016"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04552">
        <property type="protein sequence ID" value="ABA04552"/>
        <property type="gene ID" value="Nwi_1291"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05258">
        <property type="protein sequence ID" value="ABA05258"/>
        <property type="gene ID" value="Nwi_1999"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05514">
        <property type="protein sequence ID" value="ABA05514"/>
        <property type="gene ID" value="Nwi_2260"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05665">
        <property type="protein sequence ID" value="ABA05665"/>
        <property type="gene ID" value="Nwi_2411"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05690">
        <property type="protein sequence ID" value="ABA05690"/>
        <property type="gene ID" value="Nwi_2437"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06171">
        <property type="protein sequence ID" value="ABA06171"/>
        <property type="gene ID" value="Nwi_2921"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0954"/>
    <dbReference type="KEGG" id="nwi:Nwi_0976"/>
    <dbReference type="KEGG" id="nwi:Nwi_1016"/>
    <dbReference type="KEGG" id="nwi:Nwi_1291"/>
    <dbReference type="KEGG" id="nwi:Nwi_1999"/>
    <dbReference type="KEGG" id="nwi:Nwi_2260"/>
    <dbReference type="KEGG" id="nwi:Nwi_2411"/>
    <dbReference type="KEGG" id="nwi:Nwi_2437"/>
    <dbReference type="KEGG" id="nwi:Nwi_2921"/>
    <dbReference type="eggNOG" id="COG3335">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056788_10_0_5"/>
    <dbReference type="OMA" id="HISTIMR"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0814">Transposable element</keyword>
    <feature type="domain" description="DDE_3" evidence="2">
        <location>
            <begin position="71"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA04552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA05514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA05690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA06171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="238" mass="26349" checksum="FB4647CF82ECDAD1" modified="2005-10-11" version="1">MGHSRISRLKKSGSCCASAALRSAWQRCGASTTGAASASKKSVYATEQDRPDVAMAREVWKAQQGLLDPARLVFIDETGTSTNMARLRGRCRRGQRLVGKIPHGHWKITTFVAALRHDAITAPFVINEPMNGEIFRVYLQRCLVPTLKPGDIVVMDNLPAHKNDDVRQIIEAAGAQLRYLPPYSPDLNPIEMAFAKLKAHLRKAAERSIPALWNRIGSILDLFSPDQCINFFNHAGYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU24</accession>
    <name>Q3SU24_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">CRISPR-associated protein, Cas1 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_0955</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314258.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0955"/>
    <dbReference type="EnsemblBacteria" id="ABA04217">
        <property type="protein sequence ID" value="ABA04217"/>
        <property type="gene ID" value="Nwi_0955"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0955"/>
    <dbReference type="eggNOG" id="COG1518">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055263_1_0_5"/>
    <dbReference type="OMA" id="DDLMEPY"/>
    <dbReference type="OrthoDB" id="1581397at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004520">
        <property type="term" value="F:endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002729">
        <property type="entry name" value="CRISPR-assoc_Cas1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042206">
        <property type="entry name" value="CRISPR-assoc_Cas1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042211">
        <property type="entry name" value="CRISPR-assoc_Cas1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019855">
        <property type="entry name" value="CRISPR-assoc_Cas1_NMENI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01867">
        <property type="entry name" value="Cas_Cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00287">
        <property type="entry name" value="cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03639">
        <property type="entry name" value="cas1_NMENI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA04217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="238" mass="25403" checksum="ED6AECDC066B92AF" modified="2005-10-11" version="1">MVGRVVEVAGDGRHLATERGFMIVSEKAGEIGRVPLDDVAAVIANAHGLTYSNNLLVNLAARGVPVVLCGANHRPAAVVWPVDGHHAQAGRMSDQANASAPLKKRLWQQIVQAKILAQGATLAAVGAEAGGFRLLARKVRTGDPDNVEAEAARRYWRLLLGSDFRRDQDGGGINGLLNYGYAVLRAATARAVMAAGLHPSLGLKHSNRGNALVLVDDLMEPFRPAVDREVHRLRAILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU45</accession>
    <name>Q3SU45_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulatory protein, LysR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0934</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314237.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0934"/>
    <dbReference type="EnsemblBacteria" id="ABA04196">
        <property type="protein sequence ID" value="ABA04196"/>
        <property type="gene ID" value="Nwi_0934"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0934"/>
    <dbReference type="eggNOG" id="COG2005">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125440_1_0_5"/>
    <dbReference type="OMA" id="SYKRAWD"/>
    <dbReference type="OrthoDB" id="2028755at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="28"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="134" mass="14686" checksum="FAD44927FD764B0A" modified="2005-10-11" version="1">MQASSNRSLPSLSLRIDLDPKGRIGPGKIELLESIQTCGSISAAGRAMDMSYKRAWDLVDEINRICRQAAVARQTGGKNGGGAVLTPFGVSLVARYRKIERDAASAVHKELEALRNDIRHPKKSTERDRNAKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU70</accession>
    <name>Q3SU70_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0909</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314212.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0909"/>
    <dbReference type="EnsemblBacteria" id="ABA04171">
        <property type="protein sequence ID" value="ABA04171"/>
        <property type="gene ID" value="Nwi_0909"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0909"/>
    <dbReference type="eggNOG" id="COG1112">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1198">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000788_0_1_5"/>
    <dbReference type="OMA" id="KYLMWKD"/>
    <dbReference type="OrthoDB" id="616258at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021754">
        <property type="entry name" value="DUF3320"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025103">
        <property type="entry name" value="DUF4011"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="PTHR10887"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11784">
        <property type="entry name" value="DUF3320"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13195">
        <property type="entry name" value="DUF4011"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_11" evidence="1">
        <location>
            <begin position="694"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="domain" description="AAA_11" evidence="1">
        <location>
            <begin position="1335"/>
            <end position="1375"/>
        </location>
    </feature>
    <feature type="domain" description="AAA_12" evidence="2">
        <location>
            <begin position="1403"/>
            <end position="1595"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="2002" mass="222441" checksum="49E12FEA1DD8D5E9" modified="2005-10-11" version="1">MHSANCDDRQDVGVDVAEDTAEDTKAPVIVADVAASFTYASYQNAIPVIRSLLIENVSDRHFENCTLDLSSSPSFLRPKTWTIDRLLPGDKIPLSDRKVELDAGYLAGLNEAERGEITLRLSLREEVLDERRLPVRLLARDEWGGVADMAQLLPAFAMPNDPAVAPILLAAAERLAAHGHPSGLDGYQSQDPKRAYMLTAAAYSAIAGMGLHYAEPPASFENLGQKIRRPGTIAEQRLATCLDTTLLFAAALEAAGLHPVILMFRGHAAAGVWLTKRTFAQAIETDQMEVRKALASRELVVFETTGVTHRPASTFDSAQQALDRRLAEEEAHAFVAAIDIRRSRSGGITPLASHEPIADSTVDDDTAPVPALPLPAAPTFGELPVEAIEVKPTTAAGRIDRWQKKLLDLTLRNRLLNFPDTKKTIPFLCTDIAYLEDRLANGAAIRIISLPEQNPLGERDATIYREVHGRDLQRGFAAEALLRDELPSPLEAQQLEARLIDLYRQVRNDFAEGGANTLFLAVGFLRWKKKPEDERSYRAPLLLVPVKLERRSASSRFTIRFHEDEPRFNATLLQFLERVFDLTLPQFGGDLPLDDNGVDVPRLLAMMRQAIRDVPGMEVVDETALSTFSFAKFLMWKDLVERTEALRENRVVRHLIDTPEQAFSEGDGQTTFPDERELDRVYAPANIICLLPADSSQTAASLAAAEGRDFVIVGPPGTGKSQTIANMIANCLAVGKTVLFVAEKTAALDVVYRRLREHGLGNHCIELHSNKADRRHFLGQLKAAWEHGGRTDASEWVEINERLRLRRDELNAYVEALHKRYPNGWTPYHALGIALNAADAPAPAFSWPERDAHDAQTLRDLEELAAELGLVFASIERQPSLDLVDVADWSSGWQDSLLTAATSLRNAIDLLASSFEGYAAVLGLKPRERLSQTELQDLAALATALQKSRDRDITIASDREFALCAQALDGLGRSISDYREAERGLSASYGASAIRDLDPADLQRQWREANASFWPGAMLGKRKIQKLLQAYAAGGTADPERDLPLLRRMKKCLSAIDENLLVTKPLPVNGLDTDLESVANIIELARELRDALCLPNIEQDDLRSIGRSVAACLRGAAEDDRVRRAGDRLLAAIQGLESAGRRFSDLSGRDIQPGQNGSDLSTVREKLAALANTQHLLRDWAAWCRVRSRALASSLASLVERIEKGEVRPGEARVAFRLGYARWWLPKVLDADPVLRNFRRFQHENTIKEFREIDDLVRAHATRRVIGAIAHGLPPVQSVPRNSELGLLRHQMELQRPSQSIRDMIGRMPQSFSRLAPCMLMSPLSIAQYLPPNQALFDVVIFDEASQITTWDAVGAIARARQTIIVGDPKQLPPTNFFGRNEDDDEEIADYEKDLESILDEAKAAGIPVRDLRWHYRSRSESLIAFSNHHYYQNRLVTFPSPSVEDRAVQLRKVPHGVYDRGKSRTNKIEAEAVVKEAVARMKAWLRLPEKERPTLGVITFNAQQQSLILDRLDAARRDDAEIEWFFAEERVEPAIVKNLENVQGDERDVILFSITFWKDAAGILKMDFGALNRDGGERRLNVAVTRARQELIVFSGFTADQIDTSRTKAIAVQHLKTFLNYAERGAIALPAQDDGSVGGFDSPFEEAVAAQLERRGWMVVPQVGISGFRIDLGIRHPDLAGAYLAGVECDGATYHGSATARDRDKVREQVLRGLGWNILRVWSTDWWFDAAGCAGRLHAGLTSLLDQSRARRAAEQEEIATHWDMGHNVEGIDEIRDDEAAAIVDEVMPPPAPAETERVSVESPAESCKVKATTAQPDVHPMPADIPDKRQYHVTDLSSFKADPDQFFEFGYRDTLRGMIEAVIETESPLRTDILAKRISRAYGWLRTGGRIREQIDLHLRGYDTTHESSGEFIWKKGAVVDVLAYRQPVDEDARRAVADIPLAELASVALDNPDLLDDTDPVRYLARLLGVERLAAVSRARLDEAITRARQHITTVQPSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUA2</accession>
    <name>Q3SUA2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Surface antigen (D15)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0875</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314180.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0875"/>
    <dbReference type="EnsemblBacteria" id="ABA04139">
        <property type="protein sequence ID" value="ABA04139"/>
        <property type="gene ID" value="Nwi_0875"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0875"/>
    <dbReference type="eggNOG" id="COG0729">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018618_0_1_5"/>
    <dbReference type="OMA" id="ALRYWNN"/>
    <dbReference type="OrthoDB" id="470487at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000184">
        <property type="entry name" value="Bac_surfAg_D15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039910">
        <property type="entry name" value="D15-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815">
        <property type="entry name" value="PTHR12815"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01103">
        <property type="entry name" value="Omp85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="domain" description="Omp85" evidence="4">
        <location>
            <begin position="362"/>
            <end position="677"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="677" mass="72722" checksum="7CBBCB8C993ACDEB" modified="2005-10-11" version="1">MPPQSPAPSESPARRKNFIAAVRLFLCTGCAAVGTVGWSLCEPAHAFDLFGLFDSDNPPPISTAALSYRLEIEARSADGKKDSDAVQALRDASATYRLRQAPPTDGEGLVRRLQADINPLLDALWGLGRYNAKIDVTIDGVPVTLDEIDLAAAVHRAESFRNREIIPVKVVARLGPVFKLRAVDVDYPREMAPQRLPRRAFPLKPGDPAISADLRTAQLKLIDWFRSNGHPLAKVADVKATVDHAATAMDFRLRVEAGPKAGIGHVAISGSGDIDPRVVATHVHLREGEPYSPERLVLMKTSIARIPAIGGIRIREGDELDANGNVPIFIDVTERPKHAAGLSARYSTIDGPGVSTYYEHRNLFGNAERLRLDASASLLRRLDGSSSLNFGDIRASDFGGRFTATFLKPGLYGTPNDLLVEATAFRERVGNNDLGGYTNDAVRGTVGVIHRFSEMASVQAGLQIERSQSQDVLGRVDATLIGVATSGRYDTTDNLLDPTRGVRLAATINAYSKLMGSTIDLYEARIAGSTYYALDEDANYVLAGRLAAGSLVGAPLARIPDAHRFFSGGGGSVRGFIFNTISPMMFGKIIGGRSLLEGSAEMRVKVTPTIGVVPFVDFGTASSSSLPNFDEYVGYGAGIGLRYLTPVGPIRLDVATPLNPRPGDSRWAVYVSIGQAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUA3</accession>
    <name>Q3SUA3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_0874</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314179.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0874"/>
    <dbReference type="EnsemblBacteria" id="ABA04138">
        <property type="protein sequence ID" value="ABA04138"/>
        <property type="gene ID" value="Nwi_0874"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0874"/>
    <dbReference type="eggNOG" id="COG2911">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002202_0_0_5"/>
    <dbReference type="OMA" id="FKLDWKD"/>
    <dbReference type="OrthoDB" id="754526at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007452">
        <property type="entry name" value="TamB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04357">
        <property type="entry name" value="TamB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="122"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA04138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1489" mass="152688" checksum="FE0AF684376E761F" modified="2005-10-11" version="1">MRARRTIMLAAAAALGLAGVTAAGVVAYKMIGGIGDNQQDLLAGLVSRTLSTPATQVHIGAVDGALSSDATIRDVTITDKEGTWLKLDRARLVWRRTALLLGRLEIDRLEIGTLEIRRKPLAADQPVAEPEQPAVETGQPDTEPSAPAAQPGPSAAESSQSILPDLPVKVQVKAFDLHALVLGEPVLGKAVSVAATGNTELGSPSEGLVFNLDATRKDSPGRFKVELAFVPKSNALTLDVSLDEPSHGLLSKLGRFPDEPPIAFALRGKGTLDSFRGALKFQAGPTIDATGAIMLDRSGTGRVLATRINGHLGPWLPPLAASVFAGETRLDSATRIGDDGSVAMDGLTLTSRLARLEVKGALGADKMMDFSATVRSLPSNGDVTETDQGSIRSLTFDGRVNGLAKAPRVSAKLAVSDARLPDGNLDALEASINVIPDAELNDLATTVAIDASLRASGIALRDPALAAAIGDSASLVLKGSTNLGGQAKIERLEAGTSTIQAAYTGEASRDRLRGHATGAVPDLGRFGGLAGLQLAGAVRLGLDLDGDLRRGRIDAKLSGTGDRFATGLPALDGLAGPRVTLAADFSSGGNDTYTVRAASLDAAHVTVRAKGSLTRDASDLAARIELPNLAATDARLAGVASLDARLTGGLAHPGLSLTAALDHVTTMQRPIPHLGLNVTVADINGPCAIDVRLDGAIDGKPARGIVQASRRPMEHAPNTKIDQDARNKPAPAFAGWDARTIDITIGSVSLKGAGTIDAQNLARGQLRFAADSLADIAPLALIDLAGAASLDLALSDDGGRQSVRLVSKGTGLRAATTKIRNFDLRAEGDDLYSRPVLNADVRINGAEIGGQAIANATLSAKGNADASTIGLSAKAAGFNLDAAGVLTPKERIRFDLNRFTAKRGARTIALQRPASFTLDDGTVMIRHLAIGIGGGRFTLDGTAGKALDLKAVASAVPLAEADVAIPGLGLGGTLDASAEIGGSASSPTGQYKISVKRLTAPQARNAGVPPIDITLDGRLEKTRTKLNATASAGKAGTFTAAGTVPFDASETIAMTAKGRLDAAVANAMLSASGRTVSGIVQLDARVSGTRAKPQVGGFAAMSGGSFRDSQVGMRLDAIEAKVTAEGERISIERLSATTPNGGALSGSGQIRIAPDAGFPGTVSIRGKQATLAASALATARANLDVNVTGALARDPKISGRVDLTHVSVDIPERLPSTLKPIDGIDHVNATGQAAVRLAAARKAEAAEKSNGKARQALFNAALDITVSAPNHVFVHGRGVNAELGGSLRVGGTTNKPVTKGAFSLYRGKMTALGKTLNFTKGNLSFNGDLAPELDFAAEIEASDITAQIGISGPAAAPVFAFTSRPELPQDEILSRILFQKASGNLTTAQALQLAQVAAQFAGGGDGVLDRMRRSLGVDSLDVSVGSGGGPMVGASRAIGDRLSVGVRTGATPSQSGLSANVDVTRHIRVESDVDARGATSMGVGMKFEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUD1</accession>
    <name>Q3SUD1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Possible replication protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0846</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314157.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0846"/>
    <dbReference type="EnsemblBacteria" id="ABA04110">
        <property type="protein sequence ID" value="ABA04110"/>
        <property type="gene ID" value="Nwi_0846"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0846"/>
    <dbReference type="eggNOG" id="COG5534">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050846_0_0_5"/>
    <dbReference type="OMA" id="AISHIMR"/>
    <dbReference type="OrthoDB" id="355274at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018777">
        <property type="entry name" value="Replication_initiator_prot_A"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10134">
        <property type="entry name" value="RPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="287" mass="33235" checksum="4E874CBB3387D8B8" modified="2005-10-11" version="1">MSARRPYDDQQLELFRARGDDVAARDTQDLMSWPFFSLAKVRRVTPIDFRMGEVSIRVEATAEHGMATIWDADVLIWAASQIVDARDRGLRTSRLMAATPYEILTFVGRSDSGLSYDRLKAALDRLQSTTVVTSIRQPSERRRHRFSWINEWKERLDATGRPLGIELILPDWFYAGVIDDALILTIDRAYFDLTGRLERWLYRLVRKHGGRQTHGWSFDLPHLHLKSGSLSPLRRFRFELRAIVQRQPLPGYHLTLIDDPDGRQRLIFQPTASDRTVTPHQRRGGVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUF5</accession>
    <name>Q3SUF5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0821</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314134.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0821"/>
    <dbReference type="EnsemblBacteria" id="ABA04086">
        <property type="protein sequence ID" value="ABA04086"/>
        <property type="gene ID" value="Nwi_0821"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0821"/>
    <dbReference type="eggNOG" id="ENOG5033Y34">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1303834_0_0_5"/>
    <dbReference type="OrthoDB" id="2095906at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="211" mass="22936" checksum="9CE3DC3659A84AF8" modified="2005-10-11" version="1">MALNIGASGIIRPYVKYNAKSDKWFIRAEGGGDLEIARPTFLLDLANIRTGWLRFQEGQAPERLIDPALDKVAPTPGEGFKRGFVVMAFSPKFFGGAVEMASASIHVSNAIRDVYAVFEEQAGRTENRGKVPVITCTGADAMKDKYGTNYRPKLELTKWVDRPADFPDASAVEESEVWKGNAAAASKPAPVAHVPPPAAKPAPQPIYETDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUH2</accession>
    <name>Q3SUH2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0804</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314117.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0804"/>
    <dbReference type="EnsemblBacteria" id="ABA04069">
        <property type="protein sequence ID" value="ABA04069"/>
        <property type="gene ID" value="Nwi_0804"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0804"/>
    <dbReference type="eggNOG" id="ENOG5031A79">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1999652_0_0_5"/>
    <dbReference type="OrthoDB" id="1887143at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="119" mass="13709" checksum="6E7DFC2E4251C0A0" modified="2005-10-11" version="1">MLIDMMSGKTITRVPYAGEFRAFMSRMTAEEIRAIKSELNAMIEGTEIQTAGWMPGSNWGGTVYQPIYEKAARRDYSASARCFGLMVWEVFMERPERWSSGRFEKNGEPIGSRTYFRIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUH3</accession>
    <name>Q3SUH3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0803</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0803"/>
    <dbReference type="EnsemblBacteria" id="ABA04068">
        <property type="protein sequence ID" value="ABA04068"/>
        <property type="gene ID" value="Nwi_0803"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0803"/>
    <dbReference type="eggNOG" id="ENOG5032YQA">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1465561_0_0_5"/>
    <dbReference type="OrthoDB" id="1160520at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="202" mass="23551" checksum="B92E6D5809B9BA86" modified="2005-10-11" version="1">MKAQLPNIDVKYDPQNFRDLFETELGTGVWKFLIRPDNVIRMETASFLERAAVEPLAPGLVAEFGPDIAQDRIKQMIGHMVRQIMEAVGYEIDRPGLRITRESLFSSGARYQPQDHNRDRTMKITPEQRQAWLERTAKSPFNIWLDRHVKGPDGKLDLERLYEVARRHGIEKRYDHLNPGQQRMNIGVMLRSRVTQEEIEAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUI9</accession>
    <name>Q3SUI9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0787</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0787"/>
    <dbReference type="EnsemblBacteria" id="ABA04052">
        <property type="protein sequence ID" value="ABA04052"/>
        <property type="gene ID" value="Nwi_0787"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0787"/>
    <dbReference type="eggNOG" id="ENOG5032YF0">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_192049_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229135" evidence="1">
        <location>
            <begin position="26"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="84" mass="9015" checksum="2D12CCF11F7DC628" modified="2005-10-11" version="1">MPRTGCSKQALPLLVIAMLSLSACATAPSRPSVVCLTVAAYDRAFQARLADEIQRLPPGAALEQAMLDYARLRVQARACAVQTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUJ7</accession>
    <name>Q3SUJ7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_0779</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314093.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0779"/>
    <dbReference type="EnsemblBacteria" id="ABA04044">
        <property type="protein sequence ID" value="ABA04044"/>
        <property type="gene ID" value="Nwi_0779"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0779"/>
    <dbReference type="eggNOG" id="COG2223">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_14_0_5"/>
    <dbReference type="OMA" id="QMPMIFE"/>
    <dbReference type="OrthoDB" id="1217595at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015112">
        <property type="term" value="F:nitrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042128">
        <property type="term" value="P:nitrate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044772">
        <property type="entry name" value="NO3_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23515">
        <property type="entry name" value="PTHR23515"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0534">Nitrate assimilation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="218"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="262"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="319"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="354"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="379"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="17"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA04044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="418" mass="44864" checksum="D4C01859B3E9F85D" modified="2005-10-11" version="1">MEQSAGKVETSGEPQGNARVLWISTIAFSLGFAIWGMFSALAPFLIEWYHFTSTQVLVLAAMEPLFAAAISIPLGIWTDKFGGRTVFTILLVLLSVVLILGTFAEGYYSFLFLGSMLGLGGATFVVGNAHVSSWYPKSKQGTALGLFALGNIGITFGMMAVTFVITSVADQSDPEGWRIIFPIFAIPTLFMAAIYMFFTSDPPNRKLKNTSTREIWAVYRSGVVVWLVPGLYWVAFGTLVFFASTMPTYLVDHWHVDATSAVAFYTPVLVVCVAVTRPLGGWAADRYDVLTLLSWMFGAMSVLAVLMALQLSFSLELVAIYGLALLSGAAAAAVIKLIPIYFEHVGAVSGLAKAAGAACGFTMTAVLAASNFLVGGYTLGFIVWALMNMAAFYVSFSRVGFRDVKPIGAPVAEAVPAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUL6</accession>
    <name>Q3SUL6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TsaA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0760</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the tRNA methyltransferase O family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314079.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04025">
        <property type="protein sequence ID" value="ABA04025"/>
        <property type="gene ID" value="Nwi_0760"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0760"/>
    <dbReference type="eggNOG" id="COG1720">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013458_2_1_5"/>
    <dbReference type="OMA" id="QIQYFAR"/>
    <dbReference type="OrthoDB" id="1535774at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09281">
        <property type="entry name" value="UPF0066"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023370">
        <property type="entry name" value="TrmO-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040372">
        <property type="entry name" value="YaeB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036413">
        <property type="entry name" value="YaeB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036414">
        <property type="entry name" value="YaeB_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12818">
        <property type="entry name" value="PTHR12818"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01980">
        <property type="entry name" value="TrmO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118196">
        <property type="entry name" value="SSF118196"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00104">
        <property type="entry name" value="tRNA_TsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51668">
        <property type="entry name" value="TSAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="TsaA-like" evidence="3">
        <location>
            <begin position="5"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00033753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="150" mass="16903" checksum="171C03B123495708" modified="2005-10-11" version="1">MTISMRSIATVRSRFTNEVRVSRRDIVAEIIVREDLAAALTGLEEWSHLIVLFWMDQIAGKPVPLTTHPRHRTDLPEVGVFSARGRERPNPIGLAVVELLKREKNILTVKALDAYDGTPVLDIKPYDAFDAVTELRAPQWWMTATGRSSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUN4</accession>
    <name>Q3SUN4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DSBA oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0741</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be required for disulfide bond formation in some proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the thioredoxin family. DsbA subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314061.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0741"/>
    <dbReference type="EnsemblBacteria" id="ABA04007">
        <property type="protein sequence ID" value="ABA04007"/>
        <property type="gene ID" value="Nwi_0741"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0741"/>
    <dbReference type="eggNOG" id="COG1651">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_47_4_5"/>
    <dbReference type="OMA" id="YREWPIL"/>
    <dbReference type="OrthoDB" id="1805428at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13462">
        <property type="entry name" value="Thioredoxin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="3">
        <location>
            <begin position="26"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="222" mass="24003" checksum="23B270E9E8636221" modified="2005-10-11" version="1">MPESRDPFGAPTRRGLLRAFGLGMVTAGLTHAPTLAETPDNILTEARVLRDPEIPAAGNAQGDITIVEYLDFNCSYCRKLAPGLAQVVRDDGKVRLIFKDWPILGPVSVYASRLALASRYQDKFVVAHEALINTRSRLTEPRVRALMADAKIDVDRAIKDMAANAGAIDAVLKRNNDQAAAFGFNGTPSFIIGKFRVPGVLTTAQFVQAIADARKAVAAKTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUN6</accession>
    <name>Q3SUN6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0739</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314059.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0739"/>
    <dbReference type="EnsemblBacteria" id="ABA04005">
        <property type="protein sequence ID" value="ABA04005"/>
        <property type="gene ID" value="Nwi_0739"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0739"/>
    <dbReference type="eggNOG" id="ENOG503127Q">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155739_0_0_5"/>
    <dbReference type="OMA" id="FRSECKS"/>
    <dbReference type="OrthoDB" id="2088091at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011690">
        <property type="entry name" value="P_starv_induced_PsiF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07769">
        <property type="entry name" value="PsiF_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229142" evidence="1">
        <location>
            <begin position="24"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="90" mass="9314" checksum="53ACACB0416413A6" modified="2005-10-11" version="1">MTMFPRVIAVVAASLLLGATAFAQAPATEAPAAASKAASTAKKAHKTQKAQKANSTASLECTKEADAKGLRGKERKKFRAECKAKAAQPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUP1</accession>
    <name>Q3SUP1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">IS511, transposase OrfB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0734</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04000">
        <property type="protein sequence ID" value="ABA04000"/>
        <property type="gene ID" value="Nwi_0734"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0734"/>
    <dbReference type="eggNOG" id="COG2801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2495699_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13683">
        <property type="entry name" value="rve_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="80" mass="8937" checksum="F669DC058BA2E31E" modified="2005-10-11" version="1">MRDELLNETLFHSLAHARVEIAAWVEDYNRERPHSSLGYATPAAFAAELDKQWPASLRPTGSATQPIASTALMRKTTTRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUP2</accession>
    <name>Q3SUP2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Possible outer membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0733</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314053.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0733"/>
    <dbReference type="EnsemblBacteria" id="ABA03999">
        <property type="protein sequence ID" value="ABA03999"/>
        <property type="gene ID" value="Nwi_0733"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0733"/>
    <dbReference type="eggNOG" id="COG3637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037100_0_1_5"/>
    <dbReference type="OMA" id="DHTFKAG"/>
    <dbReference type="OrthoDB" id="2018542at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027385">
        <property type="entry name" value="OMP_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13505">
        <property type="entry name" value="OMP_b-brl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229262" evidence="1">
        <location>
            <begin position="21"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="OMP_b-brl" evidence="2">
        <location>
            <begin position="12"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="209" mass="22024" checksum="3BA45B235E6633CB" modified="2005-10-11" version="1">MKKILLITAAVLVSVPAASAADLAPRPYTKAPPYVPAPIYNWTGFYLGGHIGGAFSNGRDGVFLGGVQSGWDLQFAPNWVFGLEGSYSFLDSGIAGANLGLGSATARLGYTWGGPALFYVKGGYGTAVLRGNNAIFGINPNIGLDGYTVGAGLEYMFTQNWAGKVEYQYFDFGRIAGTNINLDTHTVKVGLNYRFNLGAPAAAPYAPGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUS1</accession>
    <name>Q3SUS1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Biopolymer transport protein ExbD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_0704</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the TonB-dependent energy-dependent transport of various receptor-bound substrates.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">The accessory proteins ExbB and ExbD seem to form a complex with TonB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the ExbD/TolR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314026.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0704"/>
    <dbReference type="EnsemblBacteria" id="ABA03970">
        <property type="protein sequence ID" value="ABA03970"/>
        <property type="gene ID" value="Nwi_0704"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0704"/>
    <dbReference type="eggNOG" id="COG0848">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085305_1_3_5"/>
    <dbReference type="OMA" id="TQGKKDT"/>
    <dbReference type="OrthoDB" id="1940365at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003400">
        <property type="entry name" value="ExbD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014170">
        <property type="entry name" value="TonB_ExbD_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30558">
        <property type="entry name" value="PTHR30558"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02472">
        <property type="entry name" value="ExbD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02803">
        <property type="entry name" value="ExbD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA03970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="156" mass="16592" checksum="EBB69273DE73FFD8" modified="2005-10-11" version="1">MGVSLKNPQDGGIDELSEINVTPFIDVMLVLLIIFMVAAPLSTVDVAVDLPVSNAQPQPRPDKPVFLTVKQDLTLALGNDDVPRNTLQATLDQQTENDREQRVFLRADGTVAYTELMKVMNLLREAGYLKIALVGLEDTGQAVAAGGAAPAAAPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUU3</accession>
    <name>Q3SUU3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Alkanesulfonate monooxygenase</fullName>
            <ecNumber evidence="2 6">1.14.14.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">FMNH2-dependent aliphatic sulfonate monooxygenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ssuD</name>
        <name evidence="9" type="ordered locus">Nwi_0682</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the desulfonation of aliphatic sulfonates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>an alkanesulfonate + FMNH2 + O2 = an aldehyde + FMN + 2 H(+) + H2O + sulfite</text>
            <dbReference type="Rhea" id="RHEA:23064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17359"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:134249"/>
            <dbReference type="EC" id="1.14.14.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the SsuD family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.5" evidence="2 6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314004.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0682"/>
    <dbReference type="EnsemblBacteria" id="ABA03948">
        <property type="protein sequence ID" value="ABA03948"/>
        <property type="gene ID" value="Nwi_0682"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0682"/>
    <dbReference type="eggNOG" id="COG2141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_1_0_5"/>
    <dbReference type="OMA" id="NIFWFLP"/>
    <dbReference type="OrthoDB" id="919913at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008726">
        <property type="term" value="F:alkanesulfonate monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01229">
        <property type="entry name" value="Alkanesulf_monooxygen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019911">
        <property type="entry name" value="Alkanesulphonate_mOase_FMN-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03565">
        <property type="entry name" value="alk_sulf_monoox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4 6" id="KW-0288">FMN</keyword>
    <keyword evidence="5 6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_luciferase" evidence="8">
        <location>
            <begin position="10"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="374"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA03948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="396" mass="43593" checksum="F7F00EE691FAE519" modified="2005-10-11" version="1">MTVAPAAKVKLLWFLPTHGDGRYLGTTTGGREVNFSYLRQIAQAADQLGYYGVLLPTGRSCEDSWVTASAVAPWTERLRYLVAVRPGLQSPTVAARMTATLDRVSNGRLLINVVTGGDPVENKGDGVYLDHDERYVVTREFLNVYRSLLAGESVNVDGRHIRIEDGRLLFQPVQQPYPPLYFGGSSDAGIDVAVDTVDKYLTWGEPPAQVADKVNRVRALAATRGRQLSFGIRLHVIVRETNAEAWRAADELISYVTDDTIAAAQKIFSRMDSVGQQRMAQLHGGRRDKLEISPNLWGGVGLVRGGAGTALVGDPDTVAARIREYQDIGIDTFILSGYPHLEEAYRFAELVFPLLSQPARDKVDTIRLNTGPFGETIANESRPTEARPLQRETVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUW4</accession>
    <name>Q3SUW4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TonB-dependent receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0661</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TonB-dependent receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0661"/>
    <dbReference type="EnsemblBacteria" id="ABA03927">
        <property type="protein sequence ID" value="ABA03927"/>
        <property type="gene ID" value="Nwi_0661"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0661"/>
    <dbReference type="eggNOG" id="COG4773">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008287_11_1_5"/>
    <dbReference type="OMA" id="NTAINSC"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039423">
        <property type="entry name" value="TonB-dep_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000531">
        <property type="entry name" value="TonB-dep_rcpt_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036942">
        <property type="entry name" value="TonB_rcpt_b-brl_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010917">
        <property type="entry name" value="TonB_rcpt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32552">
        <property type="entry name" value="PTHR32552"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00593">
        <property type="entry name" value="TonB_dep_Rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01156">
        <property type="entry name" value="TONB_DEPENDENT_REC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TonB_dep_Rec" evidence="2">
        <location>
            <begin position="38"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="379" mass="41842" checksum="511212ED289955D4" modified="2005-10-11" version="1">MSQNLRSRDPGTVSIHLTTDTLGANINRLDLHLLNRKNLSMWVGSMRNSIFQYNPADYPAGPYVPNNDNTYKILQKGVYGSFVAHVADPLKLIFGGRLSWYSYNSAINAVDQNTGAVTSTSLTAYEDNKVFTPYLGMVYDLTSQWSAYASFAETYQPQAFNLRGPLPGTPLGPITGRTYEVGVKGSLLDGRLNTTFALYHVKRNGEAVRDFAYPATPGDLGTNCCWLGDGRIVSQGVDIEVNGEVAAGWQVSAGYTFNDNENIAGGGRYNAFTPKHLFKLWTSYDLQGPLEGFKIGGGVTAQSSFYQTGTFAGNPFRITEPGRAVVDLFAQYRLDEHWTLALNVNNLFDRTYYQAIGAFYLGNFYGAPRNFLLSARARF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUX9</accession>
    <name>Q3SUX9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0646</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313972.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0646"/>
    <dbReference type="EnsemblBacteria" id="ABA03912">
        <property type="protein sequence ID" value="ABA03912"/>
        <property type="gene ID" value="Nwi_0646"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0646"/>
    <dbReference type="eggNOG" id="COG4123">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113234_0_0_5"/>
    <dbReference type="OMA" id="RDFAGEM"/>
    <dbReference type="OrthoDB" id="1865374at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020027">
        <property type="entry name" value="Pseudamin_synth-assoc_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03587">
        <property type="entry name" value="Pse_Me-ase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="206" mass="23555" checksum="8E66F8A31D0B389A" modified="2005-10-11" version="1">MTQITEQEQFWAGEFGDNYIDRNKSQELVAAKIAMFSRVVQRCSQIRSARELGANIGLNILALKSLLPHAAFQAIEINSRAFERLATIPGIAASNTTLFEPVEWPGVDLAFTVGVLIHLNPDMLPVAYERLYQASNRYVLTAEYYNPSVVEISYRGHSGRLWKRDFAGEMMDRFPDLKLVDYGFIYHRDPVFPADDITWFLMEKRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV16</accession>
    <name>Q3SV16_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Acyl-CoA dehydrogenase</fullName>
            <ecNumber evidence="9">1.3.8.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_0608</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.6" evidence="9"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313935.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0608"/>
    <dbReference type="EnsemblBacteria" id="ABA03875">
        <property type="protein sequence ID" value="ABA03875"/>
        <property type="gene ID" value="Nwi_0608"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0608"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_8_0_5"/>
    <dbReference type="OMA" id="AAMDCYD"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004361">
        <property type="term" value="F:glutaryl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0274">FAD</keyword>
    <keyword evidence="3 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="8">
        <location>
            <begin position="29"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="7">
        <location>
            <begin position="145"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="6">
        <location>
            <begin position="253"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA03875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="404" mass="44576" checksum="70D2AF5FD0EFE0C3" modified="2005-10-11" version="1">MSVRPQSKDKPAPVSFQWDDPFRLDDQLTEDERMIRDTARAYAQDKLLPRIVKAYQDEKTDREIFNEMGELGLNGITLPQEYGCADASYVAYGLVAREIERVDSGYRSMNSVQSSLVMYPIYAYGDENQRKKYLPRLATGEWVGCFGLTEPDAGSDPGGMKSRAEKVSDGYRLTGSKTWISNAPIADLFVVWARSAAHDNQIRGFVLEKGMKGLSAPKIGGKLSLRASVTGEIVMDGVVVPEENLLPNVSGLKGPFGCLNRARYGISWGVMGAAEDCMHRARTYVLERKQFGRPLAAAQLVQKKLADMQTDIALGLQATLRIGRLMDEGRMTPEMISIIKRNNCGKALDIARAARDMHGGNGIQIEYHVMRHAANLEAVNTYEGTHDVHALVLGRAITGIPAFS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-11-03" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV33</accession>
    <name>CYSI_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sulfite reductase [NADPH] hemoprotein beta-component</fullName>
            <shortName evidence="1">SiR-HP</shortName>
            <shortName evidence="1">SiRHP</shortName>
            <ecNumber evidence="1">1.8.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cysI</name>
        <name type="ordered locus">Nwi_0591</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3 H2O + hydrogen sulfide + 3 NADP(+) = 4 H(+) + 3 NADPH + sulfite</text>
            <dbReference type="Rhea" id="RHEA:13801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17359"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>siroheme</name>
            <dbReference type="ChEBI" id="CHEBI:60052"/>
        </cofactor>
        <text evidence="1">Binds 1 siroheme per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Alpha(8)-beta(8). The alpha component is a flavoprotein, the beta component is a hemoprotein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313919.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SV33"/>
    <dbReference type="SMR" id="Q3SV33"/>
    <dbReference type="STRING" id="323098.Nwi_0591"/>
    <dbReference type="EnsemblBacteria" id="ABA03858">
        <property type="protein sequence ID" value="ABA03858"/>
        <property type="gene ID" value="Nwi_0591"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0591"/>
    <dbReference type="eggNOG" id="COG0155">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001975_3_2_5"/>
    <dbReference type="OMA" id="MGMTHGD"/>
    <dbReference type="OrthoDB" id="1588103at2"/>
    <dbReference type="UniPathway" id="UPA00140">
        <property type="reaction ID" value="UER00207"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009337">
        <property type="term" value="C:sulfite reductase complex (NADPH)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004783">
        <property type="term" value="F:sulfite reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019344">
        <property type="term" value="P:cysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070814">
        <property type="term" value="P:hydrogen sulfide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.413.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01540">
        <property type="entry name" value="CysI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011786">
        <property type="entry name" value="CysI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005117">
        <property type="entry name" value="NiRdtase/SiRdtase_haem-b_fer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036136">
        <property type="entry name" value="Nit/Sulf_reduc_fer-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006067">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045169">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045854">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006066">
        <property type="entry name" value="NO2/SO3_Rdtase_FeS/sirohaem_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11493">
        <property type="entry name" value="PTHR11493"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01077">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03460">
        <property type="entry name" value="NIR_SIR_ferr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00397">
        <property type="entry name" value="SIROHAEM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55124">
        <property type="entry name" value="SSF55124"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56014">
        <property type="entry name" value="SSF56014"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02041">
        <property type="entry name" value="CysI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00365">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword id="KW-0349">Heme</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000388503" description="Sulfite reductase [NADPH] hemoprotein beta-component">
        <location>
            <begin position="1"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="441"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="447"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="486"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="490"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="1">
        <location>
            <position position="490"/>
        </location>
        <ligand>
            <name>siroheme</name>
            <dbReference type="ChEBI" id="CHEBI:60052"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="576" mass="64770" checksum="4ED812EDA4FEBA74" modified="2005-10-11" version="1">MDAKTQPDRSRDVSQPLDKLGPDETLKANSDYLRGTIKQSLADEITSAVTANDAKLMKFFGIYQQDDRDIRDERRRQKLEAAFSFMVRVRLPGGVCSPGQWLKLDELARNYAGDTLRLTTRQTFQFHRVMKHNLRSLIQGLRDILLDTRAACGDDTRGVMSTVNPDLSKLHAEVYALAKRASDHAVHRTGAYKEIWYEAEREQTDGPEEPFYGRTYMPRKFKIGFAIPPSNDIDVYGQDLGFIAIAHRGKLKGFNVAIGGGLGRTDQSPKTYPRLASVIGYIDADKLFPTIDAVMSVQRDYGDRLDRLHARFKYTIDEKGIDWIKAETERRLGFALKPEQPYTFTSNGDPLGWVKGEDGREHCALFIQNGRVINTPDHPMMDGLRAIAQVHKGMFRVTPNQNLIIADIASRDRPEIEALMKEYGLDQFETRSGLRLNSMACVALPTCGLAMAESERYLPDLLTKIEAILNTYGLTDDPITIRMTGCPNGCARPYIAEIGLTGRAPGKYNLYLGGGFHGERLNKMYLENVGEPAILEALDKTLGHYARDRKPGEHFGDFAIRAGYVVEVKEGRFFND</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV64</accession>
    <name>Q3SV64_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cyclic nucleotide-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0560</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0560"/>
    <dbReference type="EnsemblBacteria" id="ABA03827">
        <property type="protein sequence ID" value="ABA03827"/>
        <property type="gene ID" value="Nwi_0560"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0560"/>
    <dbReference type="eggNOG" id="COG0664">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088220_0_0_5"/>
    <dbReference type="OMA" id="SCAVITY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="SSF51206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="158"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="275" mass="30829" checksum="8437930AB40C1268" modified="2005-10-11" version="1">MWSVVLHTHGAGGGSLRLARFLPGYWIEFSITSAVFPIDRYASPSRQEIGWIAMDISHLAEHLGTVGGVAASFFVVATYTMRTMIPLRVFGMLANLMLMITALPLHNFLQFGLHAGLFFLNGYRLHQMLLLVREVKKSVNGDLSMDWLKPFMTKRKCEAGEMLFYKNEKAETMFYIVSGRYRLVESGIELPIGALVGELGMLSPSNERTQSLECIESGTVLSVSYRQVEELYVQNPEFGFYFLRLASARLFQNIGKLEDRLAETAAPPAMSPKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVE0</accession>
    <name>Q3SVE0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Inorganic pyrophosphatase</fullName>
            <ecNumber evidence="5">3.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Pyrophosphate phospho-hydrolase</fullName>
            <shortName evidence="5">PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ppa</name>
        <name evidence="6" type="ordered locus">Nwi_0484</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the hydrolysis of inorganic pyrophosphate (PPi) forming two phosphate ions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>diphosphate + H2O = H(+) + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:24576"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="EC" id="3.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313812.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0484"/>
    <dbReference type="EnsemblBacteria" id="ABA03751">
        <property type="protein sequence ID" value="ABA03751"/>
        <property type="gene ID" value="Nwi_0484"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0484"/>
    <dbReference type="eggNOG" id="COG0221">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073198_1_0_5"/>
    <dbReference type="OMA" id="DEPTFPG"/>
    <dbReference type="OrthoDB" id="1767807at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00412">
        <property type="entry name" value="pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00209">
        <property type="entry name" value="Inorganic_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008162">
        <property type="entry name" value="Pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036649">
        <property type="entry name" value="Pyrophosphatase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10286">
        <property type="entry name" value="PTHR10286"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00719">
        <property type="entry name" value="Pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50324">
        <property type="entry name" value="SSF50324"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA03751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="176" mass="19590" checksum="ED42C02497A59F85" modified="2005-10-11" version="1">MRIDAISIGPNPPHEVNVIIEVPVGGEPIKYEMDKDAGTLVVDRFLYTAMRYPGNYGFIPHTLSQDGDPCDVLVANTRAIVPGAVMSVRPVGVLMMEDEAGGDEKIIAVPTSKLTQRYDRIKNYDDLPDITLQQIQHFFEHYKDLEPGKWVKVLQWGSADDARRLILEGIARAKGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVF9</accession>
    <name>Q3SVF9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0465</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313796.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0465"/>
    <dbReference type="EnsemblBacteria" id="ABA03732">
        <property type="protein sequence ID" value="ABA03732"/>
        <property type="gene ID" value="Nwi_0465"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0465"/>
    <dbReference type="eggNOG" id="COG3502">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129452_0_0_5"/>
    <dbReference type="OMA" id="QDGFIHF"/>
    <dbReference type="OrthoDB" id="1997747at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009297">
        <property type="entry name" value="DUF952"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34129">
        <property type="entry name" value="PTHR34129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06108">
        <property type="entry name" value="DUF952"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="114" mass="12633" checksum="17D27DE425834B99" modified="2005-10-11" version="1">MRTIYKICPDSAWRQAERDGVFRGSADDVRDGFIHFSTATQVEGTARKHFVGQTGLLLIAIDADGLGEALRWEISRGGEPFPHLYGELDLRHVTAVLDMPARADGYHDIPELAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVG8</accession>
    <name>Q3SVG8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Binding-protein-dependent transport systems inner membrane component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0456</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0456"/>
    <dbReference type="EnsemblBacteria" id="ABA03723">
        <property type="protein sequence ID" value="ABA03723"/>
        <property type="gene ID" value="Nwi_0456"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0456"/>
    <dbReference type="eggNOG" id="COG0600">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046113_1_1_5"/>
    <dbReference type="OMA" id="WDGAIVF"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="30"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="198"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="254"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="92"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="285" mass="30374" checksum="696DA2E68D48D346" modified="2005-10-11" version="1">MSTSWPGSSLSGSAPPGPATFAGRAGGKPWLTLPALTNSLLGIGGLIVLLLLWWLGTDVLASSQGFIRQFSPTTAIPELVTMLGQPDLLVHVLVSLRRVLIGLALALLIGVPVGLAVGSYSRLNTATSPAFQFLRMISPLSWMPIAVMVFGVGDQPIYFMLAFAAVWPIMLNTAEGVRRLQPSWLSLADSLAATRWETLCYVILPGVLGHVLTGLRLAVGIVWIVLVPCEMLGVSAGLGYFILDTRDRLAYSELMATVLMIGVLGFLLDAGARMLYQAVTRSRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVH6</accession>
    <name>Q3SVH6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Peptidase M23B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0448</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0448"/>
    <dbReference type="EnsemblBacteria" id="ABA03715">
        <property type="protein sequence ID" value="ABA03715"/>
        <property type="gene ID" value="Nwi_0448"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0448"/>
    <dbReference type="eggNOG" id="COG4942">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029425_15_0_5"/>
    <dbReference type="OMA" id="WPATIRY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016047">
        <property type="entry name" value="Peptidase_M23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01551">
        <property type="entry name" value="Peptidase_M23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51261">
        <property type="entry name" value="SSF51261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229285" evidence="3">
        <location>
            <begin position="38"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M23" evidence="4">
        <location>
            <begin position="335"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="273"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="55"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="450" mass="47859" checksum="AD3EB70CE7C84A3F" modified="2005-10-11" version="1">MRSTEARGRQSPPASLLVISACLLAGFSSAVSSAVMAQAGTQASATPSADDIKQRELELEAIRAQQKNAVELQERLKVDIAAIGRNRAKLNQQLIDVAARVRDIETQITETEARLLPLGAREQEIRDSLDSRRSEIVEVLAALQRAGRRTPPALLVRPEDALQSLRTAILLGSVVPEMRVRAEKLVADLDELVGLRTSIAAERDRMAADRDSLKDEQTRLATLVDERQRQQSGIEKNMAAEAARAIELSRQVDTLQGLIAKLEQDLKSAAKAAATASRQGVPGSPNGKPNLGALKDPGRLSPAVAFASAKGLLALPVNGTRIRDFGGSDGAGGIEKGVSLAARPGAQVTTPCDGWVVYAGPFRSYGQLLILNAGGGYHVLIAGMERISVNIGQFVLTGEPVATMGSTSRVASILAANASQPVLYIEFRKDGTPIDPGPWWAANRGQKVRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="126" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVK3</accession>
    <name>Q3SVK3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Succinate--CoA ligase [ADP-forming] subunit alpha</fullName>
            <ecNumber evidence="2">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Succinyl-CoA synthetase subunit alpha</fullName>
            <shortName evidence="2">SCS-alpha</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">sucD</name>
        <name evidence="7" type="ordered locus">Nwi_0421</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 5">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 5">
            <text>ATP + CoA + succinate = ADP + phosphate + succinyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 5">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 5">Heterotetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the succinate/malate CoA ligase alpha subunit family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313752.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0421"/>
    <dbReference type="EnsemblBacteria" id="ABA03688">
        <property type="protein sequence ID" value="ABA03688"/>
        <property type="gene ID" value="Nwi_0421"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0421"/>
    <dbReference type="eggNOG" id="COG0074">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052104_0_0_5"/>
    <dbReference type="OMA" id="VIICITE"/>
    <dbReference type="OrthoDB" id="1081709at2"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01988">
        <property type="entry name" value="Succ_CoA_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017440">
        <property type="entry name" value="Cit_synth/succinyl-CoA_lig_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033847">
        <property type="entry name" value="Citrt_syn/SCS-alpha_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005810">
        <property type="entry name" value="CoA_lig_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="CoA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02629">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001553">
        <property type="entry name" value="SucCS_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="SSF52210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01019">
        <property type="entry name" value="sucCoAalpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01216">
        <property type="entry name" value="SUCCINYL_COA_LIG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00399">
        <property type="entry name" value="SUCCINYL_COA_LIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="1 2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="CoA_binding" evidence="6">
        <location>
            <begin position="4"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Coenzyme A binding" evidence="2">
        <location>
            <begin position="17"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Coenzyme A binding" evidence="2">
        <location>
            <begin position="96"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="2 3">
        <location>
            <position position="251"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit beta</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001553-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="294" mass="30184" checksum="CC60F8F3D09F794D" modified="2005-10-11" version="1">MPILIDRNTRVICQGFTGKNGTFHSEAAIAYGTKMVGGTSPGKGGQTHLDLPVFDTVREAREATGADASVIYVPPAGAADAICEAIDAEIPLIVCITEGIPVLDMVRVKRSLTGSKSRLIGPNCPGVVTAGECKIGIMPANIFKPGNVGIVSRSGTLTYEAVFQTSQEGLGQTTAVGIGGDPVKGTEFIDMLEMFLADPNTSAIVMIGEIGGSAEEDAAQFLIDEARRGRNKPVVGFIAGVTAPPGRRMGHAGAIISGGKGDAASKTAAMEAAGIAVSPSPARLGKTLVEKLKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVM6</accession>
    <name>Q3SVM6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PilZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0398</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313729.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0398"/>
    <dbReference type="EnsemblBacteria" id="ABA03665">
        <property type="protein sequence ID" value="ABA03665"/>
        <property type="gene ID" value="Nwi_0398"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0398"/>
    <dbReference type="eggNOG" id="ENOG503242T">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_160508_0_0_5"/>
    <dbReference type="OMA" id="GYDVCIM"/>
    <dbReference type="OrthoDB" id="1800377at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035438">
        <property type="term" value="F:cyclic-di-GMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009875">
        <property type="entry name" value="PilZ_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07238">
        <property type="entry name" value="PilZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PilZ" evidence="1">
        <location>
            <begin position="6"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="105" mass="11729" checksum="2109A4D4F7811400" modified="2005-10-11" version="1">MSWGNRKADRVTFATGYSVLMMGIDGTWQRPCTMLDISNNGARLRVKDSIEGLNLKEFFLLLSSTGLAFRRCELVRVNGDEIGVRFVEDKVPGKKKGRTLAKSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVR3</accession>
    <name>Q3SVR3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Phosphoglucomutase/phosphomannomutase</fullName>
            <ecNumber evidence="10">5.4.2.8</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_0361</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.8" evidence="10"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313693.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0361"/>
    <dbReference type="EnsemblBacteria" id="ABA03628">
        <property type="protein sequence ID" value="ABA03628"/>
        <property type="gene ID" value="Nwi_0361"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0361"/>
    <dbReference type="eggNOG" id="COG1109">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016950_9_1_5"/>
    <dbReference type="OMA" id="KCSQVMY"/>
    <dbReference type="OrthoDB" id="256504at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004615">
        <property type="term" value="F:phosphomannomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="SSF53738"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="SSF55957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PGM_PMM_I" evidence="7">
        <location>
            <begin position="27"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_II" evidence="8">
        <location>
            <begin position="176"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_III" evidence="9">
        <location>
            <begin position="278"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_IV" evidence="6">
        <location>
            <begin position="422"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA03628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="499" mass="54458" checksum="78D418A75B1A55FE" modified="2005-10-11" version="1">MFPKPKSVLAPNTYAYESEPMVKATGFREYDARWLFGKEINLMGIQALGMGLGTLIGELGQKKEIVTGHDFRSYSSSIKYALISGLLASGCKVHDIGLCMTPMAYFAQFELDVPCVAMVTASHNDNGWTGVKMGANRPLTFGPDEMTRLKEIVLGAAFAGRGGGSYEFHDNFPSRYIADLTKRPKLKRRLKAVVACGNGTAGAFAPQVMEAIGCEVIPLDTELDHSFPKYNPNPEDMKMLHAIRDAVLENKADLGLGFDGDGDRCGVVDNTGEEIFADKVGVMLARDMSAIHKDAHFVVDVKSTGLFVTDPVLQQQGARATYWKTGHSYMKRRTHELGALAGFEKSGHFFFNAPMGRGYDDGLISAIAVCEMLDRAAGKSLADLKNALPKTWSSPTMSPHCADEAKYGIVDSVVKHFEDARANGDKVAGQPIRDLVTVNGIRVTAQDGSWGLVRASSNKPELVVVVESPVSEQRMRDMFEAMDSVLRTHPEVGEYNQKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW62</accession>
    <name>Q3SW62_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Heat shock protein Hsp20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0211</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009796516.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0211"/>
    <dbReference type="EnsemblBacteria" id="ABA03479">
        <property type="protein sequence ID" value="ABA03479"/>
        <property type="gene ID" value="Nwi_0211"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0211"/>
    <dbReference type="eggNOG" id="COG0071">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046737_4_0_5"/>
    <dbReference type="OMA" id="SGNEGYP"/>
    <dbReference type="OrthoDB" id="1523369at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06470">
        <property type="entry name" value="ACD_IbpA-B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037913">
        <property type="entry name" value="ACD_IbpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="SSF49764"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="4">
        <location>
            <begin position="26"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="141" mass="15967" checksum="D4E1611A10A9CB40" modified="2005-10-11" version="1">MSRVPSLSSPFLLGFDEIERALDRVVKGADGYPPYNIERCDRDSGEPERLRITLAVAGFTRDQLDVTIEENQLVIRGRQQEDKARQYIHRGIAARHFQRTFVLAEGMLVLGADLKNGLLSIDLARPEPERVVKTIAINEHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW77</accession>
    <name>Q3SW77_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0196</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313532.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0196"/>
    <dbReference type="EnsemblBacteria" id="ABA03464">
        <property type="protein sequence ID" value="ABA03464"/>
        <property type="gene ID" value="Nwi_0196"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0196"/>
    <dbReference type="eggNOG" id="ENOG502Z9NQ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_891238_0_0_5"/>
    <dbReference type="OMA" id="PVTVVQW"/>
    <dbReference type="OrthoDB" id="968453at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229296" evidence="1">
        <location>
            <begin position="23"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="312" mass="33955" checksum="BD6BD1A81F21C33B" modified="2005-10-11" version="1">MRHFLHVFAILCLLSGANPSLASDVIYAPGSRIGLVPLIGLNPAKTFPGFETADQRVKVVVAELPAAAFRDVETVAKSNAVDPRGPKPQGLETASGQGYFIVESAKNGEDNVRRYSMVISGGTFSGYVAAQVPENVAKIYSDEAVQKMFATVALRKEVPLEEQLGLLPFKLSELSSFKLIRTLTPGAVILLSDAETENNIDSRAFIIISPIGSVPERPDDRSRFARDVAATIPNLREGRITMSEPIRINGSPGFETRIDGTNTRTNTPVTVVQWLVFGSGKTAMRLIGSTPRSEWSDVFPRFRAVRDGIRPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW88</accession>
    <name>Q3SW88_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0185</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313521.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0185"/>
    <dbReference type="EnsemblBacteria" id="ABA03453">
        <property type="protein sequence ID" value="ABA03453"/>
        <property type="gene ID" value="Nwi_0185"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0185"/>
    <dbReference type="eggNOG" id="ENOG50315WQ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068410_0_0_5"/>
    <dbReference type="OMA" id="MVTRRKE"/>
    <dbReference type="OrthoDB" id="1567051at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019088">
        <property type="entry name" value="CHP02186-rel_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09608">
        <property type="entry name" value="Alph_Pro_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02186">
        <property type="entry name" value="alph_Pro_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229348" evidence="2">
        <location>
            <begin position="28"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="239"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="263" mass="28890" checksum="B55F279C5884A433" modified="2005-10-11" version="1">MRTRAIATMAPLALLLAAILVAGHAHAEHLIVSVSNHRVTVTPNYSGEELVLFGSIERESDAPLPPAGYDLVVTVSGPPVDMVTRRKERWFGIWVNADSRQFLQVPAYLAAFANRPLDRIAAPDILRRQQLGLHNVLLTQRVGSDYADVVPSDPFRRAFIRLRSEHGLYRENTSAVTFLTPTLFRASIPLPAEVPTGTYTIDIKLLADGTLIAKTDTAFEIVKVGFEQFVATAARQHGMVYGVATALMALTAGWMASIVFRRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW94</accession>
    <name>Q3SW94_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PTS IIA-like nitrogen-regulatory protein PtsN</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0179</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313516.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0179"/>
    <dbReference type="EnsemblBacteria" id="ABA03447">
        <property type="protein sequence ID" value="ABA03447"/>
        <property type="gene ID" value="Nwi_0179"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0179"/>
    <dbReference type="eggNOG" id="COG1762">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072531_5_2_5"/>
    <dbReference type="OMA" id="FDAPDEQ"/>
    <dbReference type="OrthoDB" id="1810962at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008982">
        <property type="term" value="F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00211">
        <property type="entry name" value="PTS_IIA_fru"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016152">
        <property type="entry name" value="PTrfase/Anion_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002178">
        <property type="entry name" value="PTS_EIIA_type-2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006320">
        <property type="entry name" value="PTS_Nitro_regul"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00359">
        <property type="entry name" value="PTS_EIIA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55804">
        <property type="entry name" value="SSF55804"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01419">
        <property type="entry name" value="nitro_reg_IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51094">
        <property type="entry name" value="PTS_EIIA_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00372">
        <property type="entry name" value="PTS_EIIA_TYPE_2_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PTS EIIA type-2" evidence="1">
        <location>
            <begin position="5"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="153" mass="16606" checksum="403E2D6A462C6347" modified="2005-10-11" version="1">MMITDLVAPEAILPALKVTSKKQALQELAARAAELTGQNDRAIFEVLLRREKLGTTAVGYGVAIPHGKLQKLEKIFGLFARLERSIDFEAMDGQPVDLIFLLLAPEGAGADHLKALARIARLLRDQDIANKLRASRDAEAIYSVLALPPATVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWB1</accession>
    <name>Q3SWB1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Probable multidrug resistance protein NorM</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0162</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Multidrug efflux pump.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313499.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0162"/>
    <dbReference type="EnsemblBacteria" id="ABA03430">
        <property type="protein sequence ID" value="ABA03430"/>
        <property type="gene ID" value="Nwi_0162"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0162"/>
    <dbReference type="eggNOG" id="COG0534">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012893_5_0_5"/>
    <dbReference type="OMA" id="NSMAGQN"/>
    <dbReference type="OrthoDB" id="1415498at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006603">
        <property type="entry name" value="DinF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="87"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="162"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="240"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="285"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="319"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="358"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="391"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="418"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="449" mass="47831" checksum="502F5AC640FE8EE9" modified="2005-10-11" version="1">MMTTQPLWRAYVLFLMPMMLSNVLQSLFGTVNGIYLGQMIGVDALAAVSIFFPVMFFFLSFILGLGAGSSMLIGQAWGAREAGKAKAIAGTTLALTLLGGGVIAVGGGLFARELMMALAVPSNILDDATDYARIMMITMPAIFTFLLATAMMRGVGDTVTPLWALAISTAIGLVVTPALIRGWFGLPRLGVVSAAIASAISAVVTLLWLGLHMRWMNHPLAPDKVLLRAMRLDRELLGKVLRIGIPTAVQLMAMSLAEIVLLGIVNGFGSDATAAYGAVNQVLGYAQFPAISIAITASIFAAQAIGAGESERIGAVVRTAMWLNLLMTGGLVVLGYLFSRTIVGLFITSPEVLELAQTLLYIVLWSTVPFGMACVFAATMRASGTVMVPTLLSILAIVVVEVPVAIVLSRQIGVEGVWIAYPAAFCAMMALQMAFYLMVWRKQEIRRLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="128" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWC0</accession>
    <name>Q3SWC0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Orotate phosphoribosyltransferase</fullName>
            <shortName evidence="4">OPRT</shortName>
            <shortName evidence="4">OPRTase</shortName>
            <ecNumber evidence="2 4">2.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pyrE</name>
        <name evidence="6" type="ordered locus">Nwi_0153</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>diphosphate + orotidine 5'-phosphate = 5-phospho-alpha-D-ribose 1-diphosphate + orotate</text>
            <dbReference type="Rhea" id="RHEA:10380"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1 4">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.10" evidence="2 4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313490.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0153"/>
    <dbReference type="EnsemblBacteria" id="ABA03421">
        <property type="protein sequence ID" value="ABA03421"/>
        <property type="gene ID" value="Nwi_0153"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0153"/>
    <dbReference type="eggNOG" id="COG0461">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074878_2_1_5"/>
    <dbReference type="OMA" id="YVNCKPV"/>
    <dbReference type="OrthoDB" id="1280396at2"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00119"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004588">
        <property type="term" value="F:orotate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01208">
        <property type="entry name" value="PyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023031">
        <property type="entry name" value="OPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004467">
        <property type="entry name" value="Or_phspho_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00336">
        <property type="entry name" value="pyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 4" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pribosyltran" evidence="5">
        <location>
            <begin position="48"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="5-phosphoribose 1-diphosphate binding" evidence="4">
        <location>
            <begin position="128"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA03421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="187" mass="20310" checksum="42E13449A6096EBF" modified="2005-10-11" version="1">MPEDIKVRQRAFEIIKTRSFGRGNIKLASGKTSTFYFDMKPTMLAPEGAHVLSELILARLAGQGIDYIGGLEMGAVPLISTVSLLSHIRNEPLPGFFVRKDVKKHGTRKLIEGLPVDDLRGKRVVILEDVTTSGASAMIAVSAAQAEGAEVVMVLSIVDRGEGAMALYKEKSIEFDSLFKAEEFLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWC7</accession>
    <name>Q3SWC7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0146</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313483.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03414">
        <property type="protein sequence ID" value="ABA03414"/>
        <property type="gene ID" value="Nwi_0146"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0146"/>
    <dbReference type="eggNOG" id="ENOG5033EUT">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144140_0_0_5"/>
    <dbReference type="OMA" id="TYEHTIS"/>
    <dbReference type="OrthoDB" id="1738766at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="153" mass="17334" checksum="6E1C5F6896F9CDB1" modified="2005-10-11" version="1">MSDTIRLARTDTYEHTISGLLKKRADLFNEAERIRDRMAEIKNDIGALDRVLGTLGYTGDLNAEMPRQKRQVLFGRGELTRAILDELRDAPRPLGSREIAQAIVALNGQDARDRRALTDVTKRVSKALRGLTEQGALTHKTGEGRQRLWEIRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-01-10" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWF1</accession>
    <name>HIS6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Imidazole glycerol phosphate synthase subunit HisF</fullName>
            <ecNumber evidence="1">4.3.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">IGP synthase cyclase subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">IGP synthase subunit HisF</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">ImGP synthase subunit HisF</fullName>
            <shortName evidence="1">IGPS subunit HisF</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisF</name>
        <name type="ordered locus">Nwi_0122</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H(+) + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="4.3.2.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of HisH and HisF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HisA/HisF family.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.10" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313459.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWF1"/>
    <dbReference type="SMR" id="Q3SWF1"/>
    <dbReference type="STRING" id="323098.Nwi_0122"/>
    <dbReference type="EnsemblBacteria" id="ABA03390">
        <property type="protein sequence ID" value="ABA03390"/>
        <property type="gene ID" value="Nwi_0122"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0122"/>
    <dbReference type="eggNOG" id="COG0107">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048577_4_0_5"/>
    <dbReference type="OMA" id="IFHYKET"/>
    <dbReference type="OrthoDB" id="1522718at2"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000107">
        <property type="term" value="F:imidazoleglycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04731">
        <property type="entry name" value="HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01013">
        <property type="entry name" value="HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004651">
        <property type="entry name" value="HisF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00735">
        <property type="entry name" value="hisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000142189" description="Imidazole glycerol phosphate synthase subunit HisF">
        <location>
            <begin position="1"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01013"/>
        </source>
    </evidence>
    <sequence length="258" mass="27791" checksum="56D3CC5FF04972AA" modified="2005-10-11" version="1">MFKVRVIPCLDVKDGRVVKGVNFVDLRDAGDPVEAAIAYDAAGADELTFLDITATHENRGIMLDVVRRTAEACFMPVTVGGGVRTIEDIRTLLRSGADKVSINSAAVSRREFVKEASEKFGDQCIVVAIDAKRVSRTGGTDRWEIFTHGGRRSTGIDAIEYAQEVASLGAGEILLTSMDRDGTRQGFDLPLTRRVADSVPVPVIASGGVGNLDHLVDGIREGHATAVLAASIFHFGEFTIRQAKEHMVRAGLAMRLDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWI8</accession>
    <name>Q3SWI8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Exodeoxyribonuclease III xth</fullName>
            <ecNumber evidence="3">3.1.11.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0085</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.2" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0085"/>
    <dbReference type="EnsemblBacteria" id="ABA03353">
        <property type="protein sequence ID" value="ABA03353"/>
        <property type="gene ID" value="Nwi_0085"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0085"/>
    <dbReference type="eggNOG" id="COG0708">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027539_0_0_5"/>
    <dbReference type="OMA" id="HTPIEVE"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008853">
        <property type="term" value="F:exodeoxyribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09086">
        <property type="entry name" value="ExoIII-like_AP-endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020847">
        <property type="entry name" value="AP_endonuclease_F1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037493">
        <property type="entry name" value="ExoIII-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43250">
        <property type="entry name" value="PTHR43250"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="SSF56219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00633">
        <property type="entry name" value="xth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00726">
        <property type="entry name" value="AP_NUCLEASE_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51435">
        <property type="entry name" value="AP_NUCLEASE_F1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endo/exonuclease/phosphatase" evidence="2">
        <location>
            <begin position="31"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="295" mass="33678" checksum="B3938B006E42A7A1" modified="2005-10-11" version="1">MWERVPEHPRGRVKVRTIDRTKHPMHLTLTTWNINSVRLRIDLVARFLKAQRPDVLCLQETKCPDDAFPLKRFHRLGYEHIALNGQKGYHGVAVVSKLPFDASNIRTFCDKIDSRHVSVILGGEAGLAAPLVLHNFYVPAGGDIPDPALNPKFAHKLQFLDEMKGCAPLHPDNGDRHVLVGDLNVAPHENDVWSHKQLLKIVSHTPIECEKLLGVQDRGNWVDIARARIPMSEKVYTWWSYRAADWVAANRGRRLDHIWVSEALRDGIRDFRITRDARGWERPSDHVPVTAVIEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWJ6</accession>
    <name>Q3SWJ6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GYF_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0077</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313414.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0077"/>
    <dbReference type="EnsemblBacteria" id="ABA03345">
        <property type="protein sequence ID" value="ABA03345"/>
        <property type="gene ID" value="Nwi_0077"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0077"/>
    <dbReference type="eggNOG" id="ENOG5033A46">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_899044_0_0_5"/>
    <dbReference type="OMA" id="MRWICRN"/>
    <dbReference type="OrthoDB" id="884898at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035445">
        <property type="entry name" value="GYF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025640">
        <property type="entry name" value="GYF_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14237">
        <property type="entry name" value="GYF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55277">
        <property type="entry name" value="SSF55277"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="GYF_2" evidence="2">
        <location>
            <begin position="6"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="294" mass="32865" checksum="027CE9DF335575EB" modified="2005-10-11" version="1">MPNRQWFFASGNTQQGPYSQDQLRDFIARGAVRADTRVWTEGMSDWQRAGDIPGLLSGGSAAPAMPPASARESEPLARGTEPIGAPLSVDLNIWGLLGRTLIFVIGFLLVLPAPWVATSFYGWLISRVRVPQRPNLSFTGQPTDIWWVFVLLALCAYSGLTDIPYIGIILIPVQAWLSWMTIKWVVANISANGQPLRLSFRGEALHYIGWFVLLNLSFITIIGWAWVTTAMTRWMFRNIDGTRREIIFNASGLEVLWRTVVVAIGCILIIPIPWVLGWIARWYVSQVALAPRTA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWL9</accession>
    <name>TRPA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tryptophan synthase alpha chain</fullName>
            <ecNumber evidence="1">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trpA</name>
        <name type="ordered locus">Nwi_0054</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + H2O + L-tryptophan</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TrpA family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313393.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWL9"/>
    <dbReference type="SMR" id="Q3SWL9"/>
    <dbReference type="STRING" id="323098.Nwi_0054"/>
    <dbReference type="PRIDE" id="Q3SWL9"/>
    <dbReference type="EnsemblBacteria" id="ABA03322">
        <property type="protein sequence ID" value="ABA03322"/>
        <property type="gene ID" value="Nwi_0054"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0054"/>
    <dbReference type="eggNOG" id="COG0159">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016734_0_0_5"/>
    <dbReference type="OMA" id="LVMTYWN"/>
    <dbReference type="OrthoDB" id="912786at2"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04724">
        <property type="entry name" value="Tryptophan_synthase_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00131">
        <property type="entry name" value="Trp_synth_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018204">
        <property type="entry name" value="Trp_synthase_alpha_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002028">
        <property type="entry name" value="Trp_synthase_suA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43406">
        <property type="entry name" value="PTHR43406"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00290">
        <property type="entry name" value="Trp_syntA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00262">
        <property type="entry name" value="trpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00167">
        <property type="entry name" value="TRP_SYNTHASE_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="chain" id="PRO_1000018240" description="Tryptophan synthase alpha chain">
        <location>
            <begin position="1"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00131"/>
        </source>
    </evidence>
    <sequence length="278" mass="28779" checksum="257FEF8A97E53F1B" modified="2005-10-11" version="1">MTTRIDTRFAELRKQGRSAFVTFLMAGDPDPATSLAIIKALPRAGADIIEIGMPFTDPMADGPAVQAAGRRALDAGMTLTGTLRMIGEFRKDDDSTPVVLMGYYNPVYIYGVEKFLDDARAAGVDGLIVVDLPPEEDSELCIPAMKAGLNFIRLATPTTDDKRLPAVLANTSGFVYYVSITGITGSAAADSTAVGAAVGRIKRHTSLPVCVGFGIRTPDAARGIAERSDGAVVGSALVDALSASLDAEGKATSGTVQAVASLAAALAEGVRSARQAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWN4</accession>
    <name>Q3SWN4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0039</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313378.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0039"/>
    <dbReference type="EnsemblBacteria" id="ABA03307">
        <property type="protein sequence ID" value="ABA03307"/>
        <property type="gene ID" value="Nwi_0039"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0039"/>
    <dbReference type="eggNOG" id="COG0265">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_588841_0_0_5"/>
    <dbReference type="OMA" id="VRVICVR"/>
    <dbReference type="OrthoDB" id="741829at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.101.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036366">
        <property type="entry name" value="PGBDSf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="SSF47090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="SSF50494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229206" evidence="2">
        <location>
            <begin position="21"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="447" mass="46025" checksum="CF62224D0E825474" modified="2005-10-11" version="1">MRATIAAVLMIVATTMYAQAQPGNPVGRTKGKPEAAVSPPAQTPADTVRAMASAARQALQSDLAWTGHYNGLINGEVSDRMIAAIKAFQKERAGKQTGVLNPQERGVLAAAARKARANVGWKIAADAATGVRLGLPMRLTPQQSSAGDGTTWSSSTGTIQISVTRRKESGLTTARLADHARKQSPGRKVTYSAVKPDVFVLSGTQGLKKFYMRGQLRDSEARILTILYDQATEGVMEPVVIAMSSAFDPFPANGPPPRKLVEYASGVIVSDGGAILTGHEATDGCRSIVVTGHGYADRIAGDKDHGLALLRVYGAHGLQPIPLGRAAAKGGVDLVGITDPQNQGGGSAISRVKASVSGGDGGELMLTPAPASGFSGAAALDANGKLAGLALVKPADIAGLSGATPPAQAVLAPAQALRAFLKANDLDPFDGSFDAEAAVVRVICVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMW7</accession>
    <name>Q3SMW7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADPH-dependent FMN reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_3127</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316282.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3127"/>
    <dbReference type="EnsemblBacteria" id="ABA06374">
        <property type="protein sequence ID" value="ABA06374"/>
        <property type="gene ID" value="Nwi_3127"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3127"/>
    <dbReference type="eggNOG" id="COG0431">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055322_0_1_5"/>
    <dbReference type="OMA" id="INAMENI"/>
    <dbReference type="OrthoDB" id="1818437at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014063">
        <property type="entry name" value="Arsenate-R_ArsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43590">
        <property type="entry name" value="PTHR43590"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02690">
        <property type="entry name" value="resist_ArsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN_red" evidence="1">
        <location>
            <begin position="30"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="238" mass="26858" checksum="616AF835AFAB3BED" modified="2005-10-11" version="1">MLPNLPNVDADCIAVPRIDRLRATPSTHPPRILLLYGSLRERSYSRFLTQEAERLLKHFGAETRIFDPHGLPLPDGAAIDHPKVRELRKLSLWSEGQVWTSPERHGAMSAVMKAQIDWIPLSVGAVRPTQGRTLAVMQVSGGSQSFNAVNQMRVLGRWMRMVTIPNQSSVAKAYQEFDEAGRMKPCSYYDRVVDVMEELVKFTLLTRDVSAYLTDRYSERKESGEALMRRVNLSAASS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN92</accession>
    <name>SLYX_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein SlyX homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">slyX</name>
        <name type="ordered locus">Nwi_2999</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SlyX family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316171.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SN92"/>
    <dbReference type="SMR" id="Q3SN92"/>
    <dbReference type="STRING" id="323098.Nwi_2999"/>
    <dbReference type="EnsemblBacteria" id="ABA06249">
        <property type="protein sequence ID" value="ABA06249"/>
        <property type="gene ID" value="Nwi_2999"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2999"/>
    <dbReference type="eggNOG" id="COG2900">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_180796_5_3_5"/>
    <dbReference type="OMA" id="ITRPPHY"/>
    <dbReference type="OrthoDB" id="2039245at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00715">
        <property type="entry name" value="SlyX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007236">
        <property type="entry name" value="SlyX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36508">
        <property type="entry name" value="PTHR36508"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04102">
        <property type="entry name" value="SlyX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000227070" description="Protein SlyX homolog">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00715"/>
        </source>
    </evidence>
    <sequence length="70" mass="8042" checksum="11B09B50C18F0532" modified="2005-10-11" version="1">MTDETFADRIDALEMRATYQEEAIETLNQVVTTQWKQIDALMRQIAEIGERLREAEAARPAPANEPPPHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNC0</accession>
    <name>Q3SNC0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">OMP_b-brl domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2971</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2971"/>
    <dbReference type="EnsemblBacteria" id="ABA06221">
        <property type="protein sequence ID" value="ABA06221"/>
        <property type="gene ID" value="Nwi_2971"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2971"/>
    <dbReference type="eggNOG" id="COG3637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057473_0_0_5"/>
    <dbReference type="OMA" id="DLGTWWC"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027385">
        <property type="entry name" value="OMP_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13505">
        <property type="entry name" value="OMP_b-brl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229052" description="OMP_b-brl domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="OMP_b-brl" evidence="2">
        <location>
            <begin position="22"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="284" mass="30000" checksum="6AAE532E90C9170B" modified="2005-10-11" version="1">MRSVKLLVAAGAASLFSSAAFAADLPIAPPPPPYFVPPIVDFGGWYLRGDIGFSNQRVRNIAMADGRNAQLLSHEQTTAFDAAGIYGVGVGYQFNNWLRGDITGQYRGNANFKGTDLLTYPVGGGVVGTGVNNYNAAKSEWLVLANAYVDLGTWWGITPFIGAGVGAARVTIANYTDVGMVNNIGFGWGTYPSAAVAPAASTWNFAWAAHAGLAYQVNPGLAIELGYSYVNLGSGTTGPVSTYTGFTRGVPMQFNDITSHDLRLGVRWYLNSPPAYAPPLVTKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNJ0</accession>
    <name>Q3SNJ0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2901</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316081.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2901"/>
    <dbReference type="EnsemblBacteria" id="ABA06151">
        <property type="protein sequence ID" value="ABA06151"/>
        <property type="gene ID" value="Nwi_2901"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2901"/>
    <dbReference type="eggNOG" id="ENOG50301Y0">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147855_1_0_5"/>
    <dbReference type="OMA" id="AVICHAE"/>
    <dbReference type="OrthoDB" id="2046489at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021333">
        <property type="entry name" value="DUF2946"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11162">
        <property type="entry name" value="DUF2946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004228993" evidence="1">
        <location>
            <begin position="36"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="123" mass="12895" checksum="A165DEFAFEEA61F5" modified="2005-10-11" version="1">MRRRLGRFLPIVMFAMLVQIFAPVAACLAFNPATADPLVGAICSHASDPSSPQDRENDPQQMHGGACALCCVIQAATPPADPQAFAVGIERDASTVIWRGAVFGPPPFPVDANAQARAPPSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNJ7</accession>
    <name>Q3SNJ7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2894</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316074.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06144">
        <property type="protein sequence ID" value="ABA06144"/>
        <property type="gene ID" value="Nwi_2894"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2894"/>
    <dbReference type="eggNOG" id="COG1416">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127515_1_0_5"/>
    <dbReference type="OMA" id="QNDPAVM"/>
    <dbReference type="OrthoDB" id="1829042at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="SSF75169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229055" evidence="1">
        <location>
            <begin position="21"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="156" mass="17573" checksum="4202910DF5B19133" modified="2005-10-11" version="1">MRHIIAAAGLLIAFTSHGFATESQPVDTQPPEPQHRLVIQVDHNDRHTMDFALTNATDVIEYYRGLDQTVAVEIMACGPGLHMLRIDTSPVKERIKRIRESASPSRIQFSACNNTKENMEKKEGRPLEMLPGTMIVPLGVVRLLELQEKGWSYVRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNL7</accession>
    <name>Q3SNL7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Prok-JAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2874</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316056.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2874"/>
    <dbReference type="EnsemblBacteria" id="ABA06124">
        <property type="protein sequence ID" value="ABA06124"/>
        <property type="gene ID" value="Nwi_2874"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2874"/>
    <dbReference type="eggNOG" id="COG0476">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_787152_0_0_5"/>
    <dbReference type="OrthoDB" id="555145at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028090">
        <property type="entry name" value="JAB_dom_prok"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14464">
        <property type="entry name" value="Prok-JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Prok-JAB" evidence="6">
        <location>
            <begin position="224"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA06124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="352" mass="37794" checksum="64235730DE31AB14" modified="2005-10-11" version="1">MGCFAHTTWHGTRWPARRLPVQELATELALLGQSPAVHDGDLVVDLPKNKQRKTFLPKEARYAVNTTASLAVREALSVIGSKQAKARFAEAALFGRGDGGFLLLEGVARNPTLCDLTDELNATVTDDRVRKLLFDPEFGLTEIQIGQGCGSLTMPMTDMRLSAMTAALTEELVGLMQADEDPGCIVVGTRAEGSSNTTWTRERVAPFEVVSIEGPQGWTVRASSRVMTKIAEEVARHPAVETGGVLVGTCSARLRTIIVVDLIEAPRDSVRSATRFVLGTAGLKAAIKARHRTSGGTLFDVGTWHSHLADQGPSALDRATARQLAAERPPPSVLLIQAPTRLYALMHNGAAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNQ2</accession>
    <name>Q3SNQ2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2837</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316029.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2837"/>
    <dbReference type="EnsemblBacteria" id="ABA06089">
        <property type="protein sequence ID" value="ABA06089"/>
        <property type="gene ID" value="Nwi_2837"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2837"/>
    <dbReference type="eggNOG" id="COG4422">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1407501_0_0_5"/>
    <dbReference type="OrthoDB" id="510345at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031009">
        <property type="entry name" value="Tcm_partner"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04474">
        <property type="entry name" value="tcm_partner"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="193" mass="21896" checksum="56C6E9387A8D4548" modified="2005-10-11" version="1">MRLNINHYQGREQSFIKHLFLQKYLENAAYKLFQGRSPVFNFVDAFAGPWRNSDSESLSDTSFALALTTLENVRGILTNLGHSNLRVRFRFCEKNPESLAKLQAFATSKTDFDIKVFSGAFEDNLSGIQAACSDVRDSFTFTFIDPTGWNVDSRPIFSFLKDLRGEVLFNFMSEHVKSCGMGWCCDIGRALPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNQ8</accession>
    <name>Q3SNQ8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2830</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316023.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2830"/>
    <dbReference type="EnsemblBacteria" id="ABA06083">
        <property type="protein sequence ID" value="ABA06083"/>
        <property type="gene ID" value="Nwi_2830"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2830"/>
    <dbReference type="eggNOG" id="ENOG50333KT">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2261583_0_0_5"/>
    <dbReference type="OMA" id="DRERLIW"/>
    <dbReference type="OrthoDB" id="1784507at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="103" mass="11245" checksum="EA06492C3AF854F2" modified="2005-10-11" version="1">MSAFAAALSALFADPNIGRDAVYIANGGVSVPVRVVARRADAITDFGDARLWSETTRIDLRVTEVPNPRPGDRIEIGGDAFLIQGEPVRDRERLVWTLDLRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNR2</accession>
    <name>Q3SNR2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2826</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2826"/>
    <dbReference type="EnsemblBacteria" id="ABA06079">
        <property type="protein sequence ID" value="ABA06079"/>
        <property type="gene ID" value="Nwi_2826"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2826"/>
    <dbReference type="eggNOG" id="ENOG502Z80P">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051803_0_0_5"/>
    <dbReference type="OMA" id="MPEVPRY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044000">
        <property type="entry name" value="Phage_tube_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18906">
        <property type="entry name" value="Phage_tube_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="361" mass="38046" checksum="FF086BF69455E6A3" modified="2005-10-11" version="1">MGGGGGPTARRSAGGWRGQPEGGRHPGGTALFHSRPARLNPFDKETDMARAQGARARMALAFETTYGTPPGSGYTRMPFASATLGAEQPLLNSELLGYGRDPLAPIKDAVTADGDVVVPIDAEAFGFWLKAAFGAPTTTGSSPGPYTHTFQSGSWTLPSMAIETAMPEVPRYAMYSGVVLDQLSWQMQRSGLLTATARLVAQGETVATTSGAGTPAELDLIRFGHFNGSIKRNGTALGNVVSTEITYANNLDRIETIRADGMIDGADPSIAALTGRTEVRFADSTLVSQAIGGTPCELEFAYGLTSGQSFTFTVHAVYLPRPRIEISGPQGVQASFDWQAARDAALGRMCTAVLVNDIESY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNY0</accession>
    <name>Q3SNY0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Sulfate ABC transporter, permease protein CysW</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_2758</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The complex is composed of two ATP-binding proteins (CysA), two transmembrane proteins (CysT and CysW) and a solute-binding protein (CysP).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2758"/>
    <dbReference type="EnsemblBacteria" id="ABA06011">
        <property type="protein sequence ID" value="ABA06011"/>
        <property type="gene ID" value="Nwi_2758"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2758"/>
    <dbReference type="eggNOG" id="COG4208">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_14_0_5"/>
    <dbReference type="OMA" id="NEYNFVA"/>
    <dbReference type="OrthoDB" id="1110665at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015419">
        <property type="term" value="F:ABC-type sulfate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011866">
        <property type="entry name" value="CysW_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005667">
        <property type="entry name" value="Sulph_transpt2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30406">
        <property type="entry name" value="PTHR30406"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30406:SF1">
        <property type="entry name" value="PTHR30406:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00969">
        <property type="entry name" value="3a0106s02"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02140">
        <property type="entry name" value="permease_CysW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0764">Sulfate transport</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="66"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="105"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="252"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="70"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA06011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="289" mass="31127" checksum="2F93C85C9AA99C75" modified="2005-10-11" version="1">MSEAAFAISRDRNDARSEPRWVRALLIGLCILFLTVFIVLPLTIVFAEAFAKGGQVYFAALAEPEAVSAIKLTLTVAAISVGLNLVFGLIAAWAVAKFEFPGKTLLITLIDLPFSVSPVVSGLVFVLLFGGQGFLGPWLQDHDIQILFALPGIALATTFVTFPFVSRALIPLMQEQGTAEEEAALSLGASGLQTFFRVTLPNIKWGLLYGVLLCNARAMGEFGAVSVVSGHIRGETNTMPLLVEILYNEYQFVAAFAVASLLALLALITLIAKTILEGQLDEGRQTREH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP07</accession>
    <name>Q3SP07_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">5-formyltetrahydrofolate cyclo-ligase</fullName>
            <ecNumber evidence="3">6.3.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2731</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(6S)-5-formyl-5,6,7,8-tetrahydrofolate + ATP = 5,10-methenyltetrahydrofolate + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10488"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the 5-formyltetrahydrofolate cyclo-ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.2" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315930.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2731"/>
    <dbReference type="EnsemblBacteria" id="ABA05984">
        <property type="protein sequence ID" value="ABA05984"/>
        <property type="gene ID" value="Nwi_2731"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2731"/>
    <dbReference type="eggNOG" id="COG0212">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066245_0_1_5"/>
    <dbReference type="OMA" id="GIVFDFA"/>
    <dbReference type="OrthoDB" id="1816478at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030272">
        <property type="term" value="F:5-formyltetrahydrofolate cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002698">
        <property type="entry name" value="FTHF_cligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23407:SF1">
        <property type="entry name" value="PTHR23407:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01812">
        <property type="entry name" value="5-FTHF_cyc-lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006806">
        <property type="entry name" value="FTHF_cligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02727">
        <property type="entry name" value="MTHFS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="10"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="135"/>
            <end position="143"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR006806-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="195" mass="20553" checksum="FB703699EBF2EBD4" modified="2005-10-11" version="1">MPISSDSQPKDELRAAALARRDALSAEERASAAVALAARMPPLDITSSTVVAGYAPIRSEIDPMPLMRSLAGKGAALAMPVIVARDTALVFRAWVPGAALVRGALGTFEPPANAVEVVPDIVLVPLAAFDRAGHRIGYGAGYYDRTLEALRRHKTVTAIGLAFTVQEVAQVPALSHDVRLDYVLTEAGVCRLGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP53</accession>
    <name>Q3SP53_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2685</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2685"/>
    <dbReference type="EnsemblBacteria" id="ABA05938">
        <property type="protein sequence ID" value="ABA05938"/>
        <property type="gene ID" value="Nwi_2685"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2685"/>
    <dbReference type="eggNOG" id="COG3803">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065010_2_0_5"/>
    <dbReference type="OMA" id="FPRNCFR"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010323">
        <property type="entry name" value="DUF924"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06041">
        <property type="entry name" value="DUF924"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="204" mass="23081" checksum="F7C31735F741E30A" modified="2005-10-11" version="1">MEKLSAHAWTTRTAKRCIMKSESTSLPSPADVLAFWRAAGPGKWYTKDNAFDVEVSRRFLRLWEAAGAGELAEWEKTDDGALALVIVLDQFPRNMFRSDARTFSTDDQALAVAERAIARGADTRIEADLVEFLYVPFMHSERLADQDRCIELFRTRGRPENLKFAEIHADIIRRFGRFPHRNLVLGRATNAEEQAFLDTGGFAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP94</accession>
    <name>Q3SP94_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">6-phosphogluconolactonase</fullName>
            <shortName evidence="7">6PGL</shortName>
            <ecNumber evidence="5 7">3.1.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pgl</name>
        <name evidence="9" type="ordered locus">Nwi_2644</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 7">Hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>6-phospho-D-glucono-1,5-lactone + H2O = 6-phospho-D-gluconate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12556"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58759"/>
            <dbReference type="EC" id="3.1.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 7">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 7">Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.31" evidence="5 7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2644"/>
    <dbReference type="EnsemblBacteria" id="ABA05897">
        <property type="protein sequence ID" value="ABA05897"/>
        <property type="gene ID" value="Nwi_2644"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2644"/>
    <dbReference type="eggNOG" id="COG0363">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053947_2_0_5"/>
    <dbReference type="OMA" id="YQLFEFE"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00409"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017057">
        <property type="term" value="F:6-phosphogluconolactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01400">
        <property type="entry name" value="6PGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005900">
        <property type="entry name" value="6-phosphogluconolactonase_DevB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006148">
        <property type="entry name" value="Glc/Gal-6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039104">
        <property type="entry name" value="PGLS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11054">
        <property type="entry name" value="PTHR11054"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01182">
        <property type="entry name" value="Glucosamine_iso"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01198">
        <property type="entry name" value="pgl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucosamine_iso" evidence="8">
        <location>
            <begin position="19"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="251" mass="27211" checksum="9ECCC13CC65F800D" modified="2005-10-11" version="1">MARADMTSAAPDRGFQVLPDAEAIATEAAERIILRITENATRPAICLTGGSGPKALYALLGSPRYRSRIAWDRVHWFIGDERFVSASDERNNMTMAREIFLNRCAPPGNIHPMPTDASDPDAAARLYERELQAFYGADRLDPARPLFDLVLMGLGPDGHTASLFPGDRALDETERWVVGVDRAPVEPLVPRVTLTLPALASSREMLFLVSGEAKRGILSKVRADADLPGTRARSNGITTWLTDVAAAGSTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPA8</accession>
    <name>Q3SPA8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2630</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315833.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2630"/>
    <dbReference type="EnsemblBacteria" id="ABA05883">
        <property type="protein sequence ID" value="ABA05883"/>
        <property type="gene ID" value="Nwi_2630"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2630"/>
    <dbReference type="eggNOG" id="ENOG5032NPW">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150626_0_0_5"/>
    <dbReference type="OMA" id="LAFGHVH"/>
    <dbReference type="OrthoDB" id="1713586at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021333">
        <property type="entry name" value="DUF2946"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11162">
        <property type="entry name" value="DUF2946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229154" evidence="2">
        <location>
            <begin position="33"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="141" mass="14831" checksum="0D35CBBCD618FBF2" modified="2005-10-11" version="1">MKWFRSNIKHGSRLALLALAIQFVLSFGHHHGAVALAAPAIAMQASDAAGTGTDKTLATHDSDPANASAPRPAEPTSGADQDSDHCAVCAVLALARTVLFSVPPVLPLQDAYHVLYLTAAAEFNHLESRRVAFQPRAPPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPH4</accession>
    <name>Q3SPH4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Ribosomal RNA small subunit methyltransferase E</fullName>
            <ecNumber evidence="4 11">2.1.1.193</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_2564</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">Specifically methylates the N3 position of the uracil ring of uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S ribosomal subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>S-adenosyl-L-methionine + uridine(1498) in 16S rRNA = H(+) + N(3)-methyluridine(1498) in 16S rRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42920"/>
            <dbReference type="Rhea" id="RHEA-COMP:10283"/>
            <dbReference type="Rhea" id="RHEA-COMP:10284"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74502"/>
            <dbReference type="EC" id="2.1.1.193"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the RNA methyltransferase RsmE family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.193" evidence="4 11"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315768.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2564"/>
    <dbReference type="EnsemblBacteria" id="ABA05817">
        <property type="protein sequence ID" value="ABA05817"/>
        <property type="gene ID" value="Nwi_2564"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2564"/>
    <dbReference type="eggNOG" id="COG1385">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067442_4_0_5"/>
    <dbReference type="OMA" id="TQWKGER"/>
    <dbReference type="OrthoDB" id="1490892at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006700">
        <property type="entry name" value="rRNA_ssu_MeTrfase-E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30027">
        <property type="entry name" value="PTHR30027"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04452">
        <property type="entry name" value="Methyltrans_RNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015601">
        <property type="entry name" value="MTase_slr0722"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00046">
        <property type="entry name" value="TIGR00046"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="9 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="8 11" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR015601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA05817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="257" mass="28252" checksum="4B72D0B832E9D1B1" modified="2005-10-11" version="1">MPDLDIDFRSPRLYVDAPLAAGNRVPLNRNQGNYLGNVLRLGTGTNVLVFNGRDGEWRAAISGRKRPEHLEIVAATRKQDHLRDVHYAFAPLKHARLDYMVQKAVEMGASALQPVLTRFTQVGRVNGERMRANVIEAAEQCGILSLAAVEEPAQLDRWLGRRAGERLLVFCDEAASTANPVRALETVRSVARGIDVLVGPEGGFADNERENLLNQPNILRLSLGPRILRADTAAVAVLALVQTVLGDWTEPEKPATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPH9</accession>
    <name>Q3SPH9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Inositol monophosphatase</fullName>
            <ecNumber evidence="1">3.1.3.25</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2559</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.3.25" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315763.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2559"/>
    <dbReference type="EnsemblBacteria" id="ABA05812">
        <property type="protein sequence ID" value="ABA05812"/>
        <property type="gene ID" value="Nwi_2559"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2559"/>
    <dbReference type="eggNOG" id="COG0483">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044118_3_1_5"/>
    <dbReference type="OMA" id="NYGWISE"/>
    <dbReference type="OrthoDB" id="1741160at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008934">
        <property type="term" value="F:inositol monophosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052832">
        <property type="term" value="F:inositol monophosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052833">
        <property type="term" value="F:inositol monophosphate 4-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046855">
        <property type="term" value="P:inositol phosphate dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="275" mass="29179" checksum="A8D99A0413969251" modified="2005-10-11" version="1">MAGADDADSRADLLARDRALLIDTVREAGLLALAMRRTKLWTWTKGVSSPVSEADIAVNDLIASRLGAVPDYGWLSEESADDLSRLGKKRVWIVDPIDGTRGYLAGHDEWCVSVALVEDASPLLAAVFAPATDEFFIAARGQGTTCNDLPVQATGGDDLDFSKIAGPKPLLEKLDLRPGDAALHPRISSLALRLCRVAGGGIDAAFAGGQSHDWDIAAADLIVHEANGKMTSLAGEPVLYNRAEVTHGVLVAAGHPRHARILAHVRDVRGRRAGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPI1</accession>
    <name>Q3SPI1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF374 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2557</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315761.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2557"/>
    <dbReference type="EnsemblBacteria" id="ABA05810">
        <property type="protein sequence ID" value="ABA05810"/>
        <property type="gene ID" value="Nwi_2557"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2557"/>
    <dbReference type="eggNOG" id="COG2121">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086327_1_1_5"/>
    <dbReference type="OMA" id="PAMFIGH"/>
    <dbReference type="OrthoDB" id="1756450at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07983">
        <property type="entry name" value="LPLAT_DUF374-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007172">
        <property type="entry name" value="DUF374"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04028">
        <property type="entry name" value="DUF374"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF374" evidence="1">
        <location>
            <begin position="68"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="244" mass="27499" checksum="2DA9E0C4A204DFA2" modified="2005-10-11" version="1">MRKLLRNLLRSRWFQQSAGLLAAGFLWLVWRTNRFSYDPEDVYDIVEPQMPVILGFWHGQHFMTPFIRRAHHRGKVLISRHRDGEFNALAAERLGIETIRGSGDHGSAFNRKGGVGAFKEMVRALEEGYNVALTADVPKRSRIAGLGIIMLARESGRTIMPFAMATSRFIRLNNWDRTTINLPFGRGVLVGGEPISVPSDADAQMMEMLRARLEATLNDATRRAYAKVGRPQFEAGPEYEAGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPJ7</accession>
    <name>Q3SPJ7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Riboflavin biosynthesis protein</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="12">Riboflavin kinase</fullName>
                <ecNumber evidence="12">2.7.1.26</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="12">Flavokinase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="12">FMN adenylyltransferase</fullName>
                <ecNumber evidence="12">2.7.7.2</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="12">FAD pyrophosphorylase</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="12">FAD synthase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Nwi_2541</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the phosphorylation of riboflavin to FMN followed by the adenylation of FMN to FAD.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 12">
            <text>ATP + FMN + H(+) = diphosphate + FAD</text>
            <dbReference type="Rhea" id="RHEA:17237"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.7.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>ATP + riboflavin = ADP + FMN + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14357"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57986"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 12">Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5 12">Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the ribF family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.26" evidence="12"/>
    <dbReference type="EC" id="2.7.7.2" evidence="12"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315745.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2541"/>
    <dbReference type="EnsemblBacteria" id="ABA05794">
        <property type="protein sequence ID" value="ABA05794"/>
        <property type="gene ID" value="Nwi_2541"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2541"/>
    <dbReference type="eggNOG" id="COG0196">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048437_0_1_5"/>
    <dbReference type="OMA" id="HRGHQAI"/>
    <dbReference type="OrthoDB" id="1504821at2"/>
    <dbReference type="UniPathway" id="UPA00276">
        <property type="reaction ID" value="UER00406"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00277">
        <property type="reaction ID" value="UER00407"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003919">
        <property type="term" value="F:FMN adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008531">
        <property type="term" value="F:riboflavin kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006747">
        <property type="term" value="P:FAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009398">
        <property type="term" value="P:FMN biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02064">
        <property type="entry name" value="FAD_synthetase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015864">
        <property type="entry name" value="FAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023468">
        <property type="entry name" value="Riboflavin_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002606">
        <property type="entry name" value="Riboflavin_kinase_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015865">
        <property type="entry name" value="Riboflavin_kinase_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023465">
        <property type="entry name" value="Riboflavin_kinase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22749">
        <property type="entry name" value="PTHR22749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06574">
        <property type="entry name" value="FAD_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01687">
        <property type="entry name" value="Flavokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004491">
        <property type="entry name" value="FAD_Synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00904">
        <property type="entry name" value="Flavokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82114">
        <property type="entry name" value="SSF82114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00083">
        <property type="entry name" value="ribF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 12" id="KW-0274">FAD</keyword>
    <keyword evidence="6 12" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7 12" id="KW-0288">FMN</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Flavokinase" evidence="13">
        <location>
            <begin position="187"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR004491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABA05794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="327" mass="35168" checksum="D4105DD08D693ACB" modified="2005-10-11" version="1">MTSGFTIIRDTAPADVIPRGGVVAMGNFDGVHLGHRAVIAAALRMARLRAKPALAMTFEPHPRKFFSPNTPQFRLTDETGKLRLLAGTGLAGAVVMTFDATRAGTTAQDFIHHDLIERLGVSAVAVGYDFHFGKGRVGSPSLLVSEAPRLGIEVDVQPHVDFEERPVSSSAIRMALAEAQIANATAMLGGPWFVTGEVIHGEKRGRGLGYPTANIHLDRDCGLRHGIYAVRVGHGKGKDHRRFDGVASFGRRPTFDNGAPLLEVFLFDFQGDLYGAILDVAFIGFIRDELKFDGAEALVRQMDDDAACARIQLAAAPDAFPKLGEIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPK1</accession>
    <name>Q3SPK1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Aminotransferase</fullName>
            <ecNumber evidence="3">2.6.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2537</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315741.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2537"/>
    <dbReference type="EnsemblBacteria" id="ABA05790">
        <property type="protein sequence ID" value="ABA05790"/>
        <property type="gene ID" value="Nwi_2537"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2537"/>
    <dbReference type="eggNOG" id="COG0161">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_4_3_5"/>
    <dbReference type="OMA" id="YHGVNIA"/>
    <dbReference type="OrthoDB" id="478143at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="1 2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="445" mass="47807" checksum="8F49E2F360A3421D" modified="2005-10-11" version="1">MSDKNLALNIPNDLSAFWMPFTANRAFKKAPRLIAGAKDMHYITTDGRKVLDGAAGMWCCNAGHGRTEIAAAITRQADALDFAPPFQFGIPQAFELANRVSELAPEGLDHVFFCNSGSEAADTALKLALAYHRIAGQGSRIRLIGRERAYHGVGFGGTAVGGIVSNRKMFGSLLTGVDHLQSTYNREKQAFSKGEPQWGAELADELERLVALHGAETIAAVIVEPMAGSTGVLPAPKGYLKRLREITQKHGILLIFDEVITGFGRLGHAFAAERYGVLPDMITFAKGVTNGAAPMGGLLVHKAIHDAFMTGPANAVEIFHGYTYSAHPLACAAGLATLDIYRAEELFERARNLEPKFADAVMSLREEPNVVDIRTVGITAGIELAPNENGPGDRGFTALRAGFHDYDLMMRIAGDTLALTPPLIISEDQIGELVDKTAKVIRAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPK9</accession>
    <name>Q3SPK9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ribonuclease T2</fullName>
            <ecNumber evidence="4">3.1.27.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2529</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the RNase T2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.27.1" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315733.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2529"/>
    <dbReference type="EnsemblBacteria" id="ABA05782">
        <property type="protein sequence ID" value="ABA05782"/>
        <property type="gene ID" value="Nwi_2529"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2529"/>
    <dbReference type="eggNOG" id="COG3719">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069375_2_1_5"/>
    <dbReference type="OMA" id="WHQWKKH"/>
    <dbReference type="OrthoDB" id="1693572at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033897">
        <property type="term" value="F:ribonuclease T2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01062">
        <property type="entry name" value="RNase_T2_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.730.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001568">
        <property type="entry name" value="RNase_T2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036430">
        <property type="entry name" value="RNase_T2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018188">
        <property type="entry name" value="RNase_T2_His_AS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039378">
        <property type="entry name" value="RNase_T2_prok"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11240">
        <property type="entry name" value="PTHR11240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00445">
        <property type="entry name" value="Ribonuclease_T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55895">
        <property type="entry name" value="SSF55895"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00530">
        <property type="entry name" value="RNASE_T2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229220" evidence="3">
        <location>
            <begin position="21"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="229" mass="25365" checksum="0FB5B9C6294EF0FE" modified="2005-10-11" version="1">MRWVSRAAAASVLSWLILLAGWTADAAADQRQNAPGAFDFYVLSLSWSPSFCAAAEERGNASRSQQTQCGGRSFSFVVHGLWPQYEHGFPDYCQRPAPRLDRRIMASMLDLMPAPGLIYSQWDKHGTCSGLSAQAYFETVRKARAAVKIPAEFVDLAAPKSVTADAIEEAFVKDNPGLSRSAIAVMCDRTRLSEVRICMNKDLQFRACEEIDRRACRRDQVVMPPVRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPL0</accession>
    <name>Q3SPL0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal RNA large subunit methyltransferase J</fullName>
            <ecNumber evidence="1">2.1.1.266</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">23S rRNA (adenine(2030)-N6)-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">23S rRNA m6A2030 methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rlmJ</name>
        <name evidence="2" type="ordered locus">Nwi_2528</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically methylates the adenine in position 2030 of 23S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosine(2030) in 23S rRNA + S-adenosyl-L-methionine = H(+) + N(6)-methyladenosine(2030) in 23S rRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:43736"/>
            <dbReference type="Rhea" id="RHEA-COMP:10668"/>
            <dbReference type="Rhea" id="RHEA-COMP:10669"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74449"/>
            <dbReference type="EC" id="2.1.1.266"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RlmJ family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.266" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315732.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2528"/>
    <dbReference type="EnsemblBacteria" id="ABA05781">
        <property type="protein sequence ID" value="ABA05781"/>
        <property type="gene ID" value="Nwi_2528"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2528"/>
    <dbReference type="eggNOG" id="COG2961">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061769_0_0_5"/>
    <dbReference type="OMA" id="TYAIWYP"/>
    <dbReference type="OrthoDB" id="1186316at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036307">
        <property type="term" value="F:23S rRNA (adenine(2030)-N(6))-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00934">
        <property type="entry name" value="23SrRNA_methyltr_J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007473">
        <property type="entry name" value="RlmJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37426">
        <property type="entry name" value="PTHR37426"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04378">
        <property type="entry name" value="RsmJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="1">
        <location>
            <begin position="145"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with substrate rRNA" evidence="1">
        <location>
            <position position="3"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="284" mass="31086" checksum="4FCE8D7F1DD06D88" modified="2005-10-11" version="1">MNYRHAFHAGSFADVIKHIVLVRVLTYLHEKPAAFRVIDTHAGAGVYDLTGEEAGRGGEWLTGIARLMQARFSEAVAPLVAPYLDIVRAYNPQRGLQCYPGSPLIARALLRPQDRMVACEIEPVARKHLIGALRRDTQANVVDLDGWMALTAFVPPKERRGLVLIDPPFEREDEFERLAGGFAGAFAKWPTGCYLLWYPAKRRRATDDLARRVGAVAGGEADKCLRLEFSVAPQTADGPLTSAGLLIVNPPWTLRGELRSILPELEKPLGQGGAARFKLGAIAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPL4</accession>
    <name>Q3SPL4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Molybdopterin synthase subunit MoaD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2524</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315728.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2524"/>
    <dbReference type="EnsemblBacteria" id="ABA05777">
        <property type="protein sequence ID" value="ABA05777"/>
        <property type="gene ID" value="Nwi_2524"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2524"/>
    <dbReference type="eggNOG" id="COG1977">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114601_4_0_5"/>
    <dbReference type="OMA" id="DRVHAKP"/>
    <dbReference type="OrthoDB" id="1995573at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003749">
        <property type="entry name" value="ThiS/MoaD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02597">
        <property type="entry name" value="ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="SSF54285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="83" mass="9221" checksum="7BC79AA6983009CB" modified="2005-10-11" version="1">MKVLYFAWVRERVGKAEEALDPPPAVATVADLIAWLSQRGEEYAYAFEKPKVIRAAIDRMHVNQDTAIAGAREIAFFPPMTGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="128" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPP3</accession>
    <name>Q3SPP3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 14 15">UvrABC system protein B</fullName>
            <shortName evidence="14">Protein UvrB</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Excinuclease ABC subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">uvrB</name>
        <name evidence="21" type="ordered locus">Nwi_2495</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 14">The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA(2)B(2) complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12 14 15">Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The beta-hairpin motif is involved in DNA binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14 15">Belongs to the UvrB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2495"/>
    <dbReference type="EnsemblBacteria" id="ABA05748">
        <property type="protein sequence ID" value="ABA05748"/>
        <property type="gene ID" value="Nwi_2495"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2495"/>
    <dbReference type="eggNOG" id="COG0556">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009621_1_0_5"/>
    <dbReference type="OMA" id="EYVDRMV"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009380">
        <property type="term" value="C:excinuclease repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009381">
        <property type="term" value="F:excinuclease ABC activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00204">
        <property type="entry name" value="UvrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001943">
        <property type="entry name" value="UVR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036876">
        <property type="entry name" value="UVR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004807">
        <property type="entry name" value="UvrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041471">
        <property type="entry name" value="UvrB_inter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024759">
        <property type="entry name" value="UvrB_YAD/RRR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24029">
        <property type="entry name" value="PTHR24029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12344">
        <property type="entry name" value="UvrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17757">
        <property type="entry name" value="UvrB_inter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46600">
        <property type="entry name" value="SSF46600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00631">
        <property type="entry name" value="uvrb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 14 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 14 15" id="KW-0228">DNA excision</keyword>
    <keyword evidence="9 14 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 14 15" id="KW-0267">Excision nuclease</keyword>
    <keyword evidence="4 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 14 15" id="KW-0742">SOS response</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="19">
        <location>
            <begin position="255"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="20">
        <location>
            <begin position="660"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="domain" description="UVR" evidence="18">
        <location>
            <begin position="860"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="204"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="940"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="486"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Beta-hairpin" evidence="14">
        <location>
            <begin position="321"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="18"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="166"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="974"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="268"/>
            <end position="275"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00026033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ABA05748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1032" mass="114114" checksum="791A9210F09ADCE0" modified="2005-10-11" version="1">MAKAPNPPEKPGKAPGSRKSKASTSQTSKQQTSKPKAHRPEVQPIGPALADLLNPAINRGDAGLGSGTGLQPPPDNSWDRRSGGEAAAHRARRSTRGKSDATAKRDAASATFENPPPSQPSKSELRSSRRPARGIESGFEEAPQAIYGTSATIPTLDPELARQLGMPTAEDDEDALARPPRSKMEGLGVKATAESLEALLRDGRPEFKTGDGSLKLWTPHRPPRPEKSEGGVRFEIKSEYQPKGDQPQAIRELVEGIRRNDRTQVLLGVTGSGKTYTMAKVIEATQRPALILAPNKTLAAQLYGEFKSFFPDNAVEYFVSYYDYYQPEAYVPRTDTYIEKDSSINEQIDRMRHAATRALLERDDVIIVASVSCIYGIGSVETYTAMTFALKRGERIDQRQLIADLVALQYKRTSADFTRGTFRVRGDTIDIFPAHYEDRAWRVNLFGDEVESIEEFDPLTGHKQDELDFIKVYSNSHYVTPRPTLVQAIKNIKVELKQRLDELHAQGRLLEAQRLEQRTTFDLEMMEATGSCAGIENYSRYLTGRRPGEPPPTLFEYVPDNALVFADESHVSVPQIGGMFRGDFRRKATLAEYGFRLPSCMDNRPLRFEEWDMMRPQTIAVSATPGAWELNESGGVFVEQVIRPTGLIDPPVDIRPARTQVDDLVGEVRATAAKGYRSLVTVLTKRMAEDLTEYLHEQGIRVRYMHSDIDTIERIEIIRDLRLGAFDALVGINLLREGLDIPECALVAILDADKEGFLRSETSLIQTIGRAARNVEGRVILYADQVTGSMQRSIAETDRRREKQVEYNTAHGITPESIKKSIGDILNSVYERDHVLVEIGDGGKGNSWSDDAIAIGHNFEAVLSDLETRMREAAADLNFEEAARLRDEVKRLRATELAVVDDPTIKQRGVAAKAGSYAGKKKYGDAANLPARLDKAAQGTAAGKFGKRGSSTSKVHKPSLDEMGIATWHEVKPTGARPKPRKPTLDEMGPGTESRIYQPTSTREAGPEFGPGPRSTGGAPGKRGGWKNNRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="127" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPQ3</accession>
    <name>Q3SPQ3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Energy-dependent translational throttle protein EttA</fullName>
            <ecNumber evidence="8">3.6.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Translational regulatory factor EttA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ettA</name>
        <name evidence="11" type="ordered locus">Nwi_2485</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">A translation factor that gates the progression of the 70S ribosomal initiation complex (IC, containing tRNA(fMet) in the P-site) into the translation elongation cycle by using a mechanism sensitive to the ATP/ADP ratio. Binds to the 70S ribosome E-site where it modulates the state of the translating ribosome during subunit translocation. ATP hydrolysis probably frees it from the ribosome, which can enter the elongation phase.</text>
    </comment>
    <comment type="function">
        <text evidence="7">Involved in beta-(1--&gt;2)glucan export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer. Probably contacts ribosomal proteins L1, L5, L33 and S7, the 16S and 23S rRNA and the P-site containing tRNA(fMet).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="8">Associates with ribosomes and polysomes.</text>
    </comment>
    <comment type="domain">
        <text evidence="8">The P-site tRNA interaction motif (PtIM domain) probably interacts with the P-site tRNA(fMet) as well as the 23S rRNA.</text>
    </comment>
    <comment type="domain">
        <text evidence="8">The arm domain is inserted in the first ABC transporter domain. Probably contacts ribosomal protein L1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the ABC transporter superfamily. ABCF family. Translational throttle EttA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.-" evidence="8"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315691.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2485"/>
    <dbReference type="EnsemblBacteria" id="ABA05738">
        <property type="protein sequence ID" value="ABA05738"/>
        <property type="gene ID" value="Nwi_2485"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2485"/>
    <dbReference type="eggNOG" id="COG0488">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_36_0_5"/>
    <dbReference type="OMA" id="ISYKPQY"/>
    <dbReference type="OrthoDB" id="1112046at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045900">
        <property type="term" value="P:negative regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00847">
        <property type="entry name" value="EttA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022374">
        <property type="entry name" value="EttA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43858">
        <property type="entry name" value="PTHR43858"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03719">
        <property type="entry name" value="ABC_ABC_ChvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 8" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="6 8" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="2 8" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="7"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="319"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="PtIM" evidence="8">
        <location>
            <begin position="237"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="40"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 9">
        <location>
            <begin position="351"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA05738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="550" mass="61573" checksum="AD6E49D0AC2E42C8" modified="2005-10-11" version="1">MARQFIYFMQGLSKTYPPNRKVLDNIHLSFYPDAKIGVLGVNGSGKSTLLKIMAGLDKDYTGEAWVAEGARVGYLEQEPQLDPAKTVRENVMEGVAKHKAILDRYNELAMNYSDETADEMTRLQDEIEAAGLWDLDSKVDQAMDALRCPPDDADVTKLSGGERRRVALCKLLLDQPDLLLLDEPTNHLDAESVSWLEGHLRNYPGAILIVTHDRYFLDNVTGWILELDRGRGIPYEGNYSSWLVQKQKRLEQEGREDAAHQKTLAREQEWIAASPKARQAKSKARYQRYEELLAKASEKQTQTAQIIIPVSERLGANVVDFEGLSKSFGDRLLIDDLTFKLPPGGIVGVIGPNGAGKTTLFRMITGQETPDKGTITVGETVQLGYVDQSRDALDGSKTVWEEISGGTDQILLGKKEVNSRAYCSTFNFKGADQQKKVGALSGGERNRVHLAKMLKSGANVLLLDEPTNDLDVDTLRALEEALEDFAGCAVVISHDRWFLDRIATHILAFEGDSHVEWFEGNFQDYEKDKMRRLGQDSVIPHRVKYKKLTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPV3</accession>
    <name>Q3SPV3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase IS3/IS911</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2408</name>
        <name evidence="3" type="ordered locus">Nwi_2434</name>
        <name evidence="4" type="ordered locus">Nwi_2864</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="3 5" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="3">Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2408"/>
    <dbReference type="EnsemblBacteria" id="ABA05662">
        <property type="protein sequence ID" value="ABA05662"/>
        <property type="gene ID" value="Nwi_2408"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05688">
        <property type="protein sequence ID" value="ABA05688"/>
        <property type="gene ID" value="Nwi_2434"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06114">
        <property type="protein sequence ID" value="ABA06114"/>
        <property type="gene ID" value="Nwi_2864"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2408"/>
    <dbReference type="KEGG" id="nwi:Nwi_2434"/>
    <dbReference type="KEGG" id="nwi:Nwi_2864"/>
    <dbReference type="eggNOG" id="COG2963">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027402_36_0_5"/>
    <dbReference type="OMA" id="SIAQCLY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002514">
        <property type="entry name" value="Transposase_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010921">
        <property type="entry name" value="Trp_repressor/repl_initiator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01527">
        <property type="entry name" value="HTH_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48295">
        <property type="entry name" value="SSF48295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA06114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="124" mass="13891" checksum="8392307E3336F84C" modified="2005-10-11" version="1">MKQKSGPGKAPAEQVLKDIRRQTRRHYSAEEKIRIVLEGLRGEENISELCRREGIAASMYYGWSKEFLEAGKRRLAGDTARAATSGEVKDLRREASALKEVVADLTLENRLLKKSMNGDGENEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPW2</accession>
    <name>Q3SPW2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">L-aspartate oxidase</fullName>
            <ecNumber evidence="4 10">1.4.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Nwi_2425</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the oxidation of L-aspartate to iminoaspartate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-aspartate + O2 = H2O2 + iminosuccinate</text>
            <dbReference type="Rhea" id="RHEA:25876"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
            <dbReference type="EC" id="1.4.3.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:25877"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (oxidase route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.16" evidence="4 10"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315637.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2425"/>
    <dbReference type="EnsemblBacteria" id="ABA05679">
        <property type="protein sequence ID" value="ABA05679"/>
        <property type="gene ID" value="Nwi_2425"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2425"/>
    <dbReference type="eggNOG" id="COG0029">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014312_3_2_5"/>
    <dbReference type="OMA" id="DSPDIHY"/>
    <dbReference type="OrthoDB" id="153138at2"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00326"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008734">
        <property type="term" value="F:L-aspartate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044318">
        <property type="term" value="F:L-aspartate:fumarate oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037099">
        <property type="entry name" value="Fum_R/Succ_DH_flav-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005288">
        <property type="entry name" value="NadB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42716">
        <property type="entry name" value="PTHR42716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46977">
        <property type="entry name" value="SSF46977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="SSF56425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00551">
        <property type="entry name" value="nadB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0274">FAD</keyword>
    <keyword evidence="5 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 10 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 10" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_2" evidence="11">
        <location>
            <begin position="11"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Succ_DH_flav_C" evidence="12">
        <location>
            <begin position="456"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA05679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="509" mass="52708" checksum="B13F1FEB9FDC8A23" modified="2005-10-11" version="1">MTAPHDTNGAAVVIGGGLAGLMTALALAPRPVLLLSSAPLGLETSSILAQGGIAASIGPDDNASLHLADTVAAGDGLCDQRLAAAILAAAPDAIERLLQLGVAFDRDADGNLALGLEAAHSRRRIVHAGGDASGRDIIRTLVRKVYETPSITVCEATCAQRLIVEDNAVRGVVCQTKRDSVSFVTDHIVIATGGIGGLFLHGTNPAGSCGQGLALAARAGAIMADLEFIQFHPTALDIGSFPLKLISEAVRGEGATLIDENGDRFMADTPGAELAPRDVVARAVWRHMAAGHRVFLNARHMPGVDFARRFPAITSFCRDAGIDPLTQPIPVRPAAHYHMGGVSVDNRGRTSIDGLWACGEAACTGLHGANRLASNSLLEAVVCAGLVARDIAGTTPAKRRLPRATDAGAGCDPALIRPIMSRAVGVLRDGEGLRVAARALLPLAASQQTASDPAIVALMIVIAALRREESRGAHARTDYPEPAISAARTTLRLRDAFDAAQDCIPDLVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ37</accession>
    <name>Q3SQ37_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Dihydrofolate reductase</fullName>
            <ecNumber evidence="3 7">1.5.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_2350</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(6S)-5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:15009"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 8">Belongs to the dihydrofolate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.3" evidence="3 7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315568.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2350"/>
    <dbReference type="EnsemblBacteria" id="ABA05604">
        <property type="protein sequence ID" value="ABA05604"/>
        <property type="gene ID" value="Nwi_2350"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2350"/>
    <dbReference type="eggNOG" id="COG0262">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043966_5_1_5"/>
    <dbReference type="OMA" id="RDNQLPW"/>
    <dbReference type="OrthoDB" id="1920912at2"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00158"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004146">
        <property type="term" value="F:dihydrofolate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006545">
        <property type="term" value="P:glycine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00209">
        <property type="entry name" value="DHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012259">
        <property type="entry name" value="DHFR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017925">
        <property type="entry name" value="DHFR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001796">
        <property type="entry name" value="DHFR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48069">
        <property type="entry name" value="PTHR48069"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00186">
        <property type="entry name" value="DHFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000194">
        <property type="entry name" value="DHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="SSF53597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00075">
        <property type="entry name" value="DHFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51330">
        <property type="entry name" value="DHFR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="5 7 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHFR" evidence="9">
        <location>
            <begin position="10"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA05604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="178" mass="19299" checksum="0FA48C888A28F297" modified="2005-10-11" version="1">MTGTVSPRPEVILVVAVAENGVIGQRNGIPWRLKADLRRFKAITIGKPIVMGRKTFDSLRRPLPGRTNIVITRDPDFTAVGALVARSPADALVIAKGDALRRSVAEIAVIGGAEIYAASMPAADRLEVTEVHTAVDGDTIFPPIDPSVWDEIARCRNKAAAGDTADFSYVTYRRRVRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ75</accession>
    <name>Q3SQ75_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protoporphyrinogen IX oxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">Nwi_2312</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3 A + protoporphyrinogen IX = 3 AH2 + protoporphyrin IX</text>
            <dbReference type="Rhea" id="RHEA:62000"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:57306"/>
            <dbReference type="ChEBI" id="CHEBI:57307"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the HemJ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315531.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2312"/>
    <dbReference type="EnsemblBacteria" id="ABA05566">
        <property type="protein sequence ID" value="ABA05566"/>
        <property type="gene ID" value="Nwi_2312"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2312"/>
    <dbReference type="eggNOG" id="COG1981">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125006_3_0_5"/>
    <dbReference type="OMA" id="ICHALCG"/>
    <dbReference type="OrthoDB" id="1752102at2"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00324"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005265">
        <property type="entry name" value="HemJ-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03653">
        <property type="entry name" value="UPF0093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0349">Heme</keyword>
    <keyword evidence="14" id="KW-0408">Iron</keyword>
    <keyword evidence="15 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00006501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00017504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ABA05566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="172" mass="18558" checksum="49C08C384A7480A3" modified="2005-10-11" version="1">MTIIWLKFIHVGAISLWCAGLIGLPALYGQRVRLRDKAALFTLQGSVRFSYLALISPAAFIAVGSGIALVFVAETFMPWFSLKLAVVGMLVGLHLLTGLIIVRLFDDGEDYPVWRFVAVAGASLLATLAILLLVLAKPSFPLDALPDAISRPGALRELFAPLLRWTGLEAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQA1</accession>
    <name>Q3SQA1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutamate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_2286</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate and ammonia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-glutamate + NADP(+) = 2-oxoglutarate + H(+) + NADPH + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:11612"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.4.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3 5 9">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315507.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2286"/>
    <dbReference type="EnsemblBacteria" id="ABA05540">
        <property type="protein sequence ID" value="ABA05540"/>
        <property type="gene ID" value="Nwi_2286"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2286"/>
    <dbReference type="eggNOG" id="COG0334">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025763_1_2_5"/>
    <dbReference type="OMA" id="WLQNRNG"/>
    <dbReference type="OrthoDB" id="1184827at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004354">
        <property type="term" value="F:glutamate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:cellular amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01076">
        <property type="entry name" value="NAD_bind_1_Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014362">
        <property type="entry name" value="Glu_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033922">
        <property type="entry name" value="NAD_bind_Glu_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000185">
        <property type="entry name" value="Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ELFV_dehydrog" evidence="10">
        <location>
            <begin position="184"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="352"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalysis" evidence="8">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA05540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="419" mass="45635" checksum="C595A772DCAB6466" modified="2005-10-11" version="1">MTVYAGAVFDMAVRQFGVIADHLSIPMDERERILMPKRAITISCPVHRDDGSIAVFEGYRVQHHLTLGPTKGGTRFAPCVDIGEVAALAIWMSWKCALVGLPYGGAKGGVSVDPQSLSMRELEGLSRRYMQEMIPFVGPHTDVMAPDMGTNEQVMAWFMDTYSMYQGRTVTEIVTGKPVSTGGTLGRREATGRGVAHLVRRVMKERDIAFGGATAVVQGFGNVGSQAALELYNSGVRIIAVSDHTGALHDSKGLDIPGLLRHTGTQGSLAGYSNQLSYDPAAILTLPCDVLVPAAVERVIDARVAENLKCRVLAEGANGPTTPEADLILETRQDEIFLIPDILCNSGGVVVSYFEWVQGLQQLFWEEDQVTRREYQILDRAFGQMVERAKRDGISHRTAAMTLGVEKVRSAKAKRGLFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQA5</accession>
    <name>Q3SQA5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FAD dependent oxidoreductase</fullName>
            <ecNumber evidence="2">1.4.99.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2282</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.4.99.1" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315503.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2282"/>
    <dbReference type="EnsemblBacteria" id="ABA05536">
        <property type="protein sequence ID" value="ABA05536"/>
        <property type="gene ID" value="Nwi_2282"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2282"/>
    <dbReference type="eggNOG" id="COG0665">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007884_9_0_5"/>
    <dbReference type="OMA" id="VIPYAFP"/>
    <dbReference type="OrthoDB" id="573710at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAO" evidence="1">
        <location>
            <begin position="7"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="417" mass="45809" checksum="C47E4573AE17A6A0" modified="2005-10-11" version="1">MALVKSDVLVLGAGIVGVSAALHLQKRGRDVILVDRHDGAGQETSYGNAGLIESASVFPYVFPYDLRQVLRYALNRFPEAHYRFRDLPAFLPWLIRYVFASAPERAFRSAMAALPLISRSLAEHEALIAEAGVPQLLRRTGWIKLFRSTATLDKGVADLERVRPYGVEGDILDARAIAAREPNLSGTFAGAIHWPAPGFIPDPGALTKAYAALFVRRGGRFIVADARTLTQGQAAWRVEGPNGALMGREAVIALGPWSDDIFRPLGYDMPLGIKRGYHLHLAPRGNAVLNRPVLDSDRGYLLAPMNQGIRLTTGAEFARRDAPPTPVQIERTLPLARELFPLGEAIEATPWMGRRPCMPDMLPVIGKAARHDGLWFNFGHQHHGLTLGPVTGRLLAEMMTGVEPFADPRPFAAERFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQA8</accession>
    <name>Q3SQA8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cytochrome P450</fullName>
            <ecNumber evidence="3">1.14.15.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2279</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.15.1" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315500.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2279"/>
    <dbReference type="EnsemblBacteria" id="ABA05533">
        <property type="protein sequence ID" value="ABA05533"/>
        <property type="gene ID" value="Nwi_2279"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2279"/>
    <dbReference type="eggNOG" id="COG2124">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033716_0_1_5"/>
    <dbReference type="OMA" id="DKVTLWY"/>
    <dbReference type="OrthoDB" id="816674at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018683">
        <property type="term" value="F:camphor 5-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002397">
        <property type="entry name" value="Cyt_P450_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00359">
        <property type="entry name" value="BP450"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="SSF48264"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="398" mass="44484" checksum="DEF34A122509D7BA" modified="2005-10-11" version="1">MSSPAPVTDWVHDFDHTDPRWTENPYPIWDDLRARCPIAHTNRFLGVYLPTTYEAVKEISRDTEHFSSRRVVVRNFRPEPPPPAPPITSDPPAHKHAKQILLPPFAPEAVAKLKPRIRAICNELIDGFINEKGCDAAARYTRYIPTRAITIMLGVPEKDGDLFIKWIHEILEVGLNDDAAVMQAVREMVGYFAEQIEARKKHPTDDLIATLMNARSEDGQPLTDMHVQGALRLLLVAGIDTAWSAIGSSLWHLAQTPADRERLIAEPELIPTAIEEFLRAFAPATSGREVMKETTVSGCPMKPGNMVLLSFPAANRDPAVFPDADKIIIDRKENRHIAFGVGIHRCVGAHLARMEMLVAIEEWLKRIPEFRLDPSGGVKWSEGSVRGPRQLPLLLGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQE3</accession>
    <name>Q3SQE3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2244</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315466.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05498">
        <property type="protein sequence ID" value="ABA05498"/>
        <property type="gene ID" value="Nwi_2244"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2244"/>
    <dbReference type="eggNOG" id="ENOG502ZHUH">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109797_0_0_5"/>
    <dbReference type="OMA" id="MTQRYDA"/>
    <dbReference type="OrthoDB" id="1949655at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022268">
        <property type="entry name" value="CHP03809"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03809">
        <property type="entry name" value="TIGR03809"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="164" mass="18493" checksum="8FD9535DDC90AA81" modified="2005-10-11" version="1">MTQRYDAGRAPDLAQDILNRWCVLAEQRLDFLIELYESGRWRRYHSENAFMDNLREAKAVVETWRALAQREATPDNRAFDWSRLDRPSQTPAVSQHGAGQSSAGAAIPYAPRVVVGLQEGATPRAKDEVPAGKNARDADRPTGILPDLKTLQMRYPILRYARTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQF0</accession>
    <name>Q3SQF0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glutathione-dependent formaldehyde-activating, GFA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2237</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Gfa family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315459.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2237"/>
    <dbReference type="EnsemblBacteria" id="ABA05491">
        <property type="protein sequence ID" value="ABA05491"/>
        <property type="gene ID" value="Nwi_2237"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2237"/>
    <dbReference type="eggNOG" id="COG3791">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055491_4_0_5"/>
    <dbReference type="OMA" id="WPAKGVK"/>
    <dbReference type="OrthoDB" id="1555973at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016846">
        <property type="term" value="F:carbon-sulfur lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006913">
        <property type="entry name" value="CENP-V/GFA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04828">
        <property type="entry name" value="GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="SSF51316"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51891">
        <property type="entry name" value="CENP_V_GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CENP-V/GFA" evidence="3">
        <location>
            <begin position="9"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="154" mass="16641" checksum="73B7A5E127ABE585" modified="2005-10-11" version="1">MTEQMSPSLTGGCQCGAIRFALTAPPVRISICHCRMCQKAAGAPFASFADIPRENFHWTRSTPATFRSSSIAERAFCAACGTPLSFSRIGGPCIEIMTGAFDRPDLLVPTGQYGTESRLGWIATIVNLPSHTTPENYGPDKMATIISHQHPDHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQG7</accession>
    <name>Q3SQG7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PG_binding_1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2220</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2220"/>
    <dbReference type="EnsemblBacteria" id="ABA05474">
        <property type="protein sequence ID" value="ABA05474"/>
        <property type="gene ID" value="Nwi_2220"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2220"/>
    <dbReference type="eggNOG" id="COG3409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1217934_0_0_5"/>
    <dbReference type="OMA" id="YVCVNAM"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.101.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036366">
        <property type="entry name" value="PGBDSf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="SSF47090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="PG_binding_1" evidence="3">
        <location>
            <begin position="132"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="193" mass="20426" checksum="AF4EB68B2F8850B0" modified="2005-10-11" version="1">MRPRRRGVATGKSGADEERGLAMRILMHSPKDLVAGGMAFAAAGAIIANAMFMQAGHHPSPIFGSAVTISPARQGNAVQQGNPLPRPRPVEADETGRAEPRARAANAPGNPVQQAGPHSDPVADIINANRRIAAVQRALTEYGFGQLKPTGTVGTETQAAIRKFEQARRMPVTGQMSDRLVRELSALTGRPIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQJ7</accession>
    <name>Q3SQJ7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2190</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315413.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05444">
        <property type="protein sequence ID" value="ABA05444"/>
        <property type="gene ID" value="Nwi_2190"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2190"/>
    <dbReference type="eggNOG" id="COG5425">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145215_1_0_5"/>
    <dbReference type="OMA" id="YIWQDYD"/>
    <dbReference type="OrthoDB" id="1883334at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009354">
        <property type="entry name" value="Usg"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06233">
        <property type="entry name" value="Usg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="99" mass="11528" checksum="714EE93BF00045E7" modified="2005-10-11" version="1">MVSKDDVRPGGVTDDFRKQVLGYGLTTAEILYRRPDRQWLLQSYVWQNYDMFPKFPVLCDFLAFWEKQLDGPLVSVTVAHSRLIKPAELRAVDGVFRLH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQP4</accession>
    <name>GUAA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GMP synthase [glutamine-hydrolyzing]</fullName>
            <ecNumber evidence="1">6.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">GMP synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glutamine amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">guaA</name>
        <name type="ordered locus">Nwi_2143</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the synthesis of GMP from XMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + XMP = AMP + diphosphate + GMP + 2 H(+) + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315366.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SQP4"/>
    <dbReference type="SMR" id="Q3SQP4"/>
    <dbReference type="STRING" id="323098.Nwi_2143"/>
    <dbReference type="EnsemblBacteria" id="ABA05397">
        <property type="protein sequence ID" value="ABA05397"/>
        <property type="gene ID" value="Nwi_2143"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2143"/>
    <dbReference type="eggNOG" id="COG0518">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0519">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014340_0_5_5"/>
    <dbReference type="OMA" id="KRKIIGH"/>
    <dbReference type="OrthoDB" id="504464at2"/>
    <dbReference type="UniPathway" id="UPA00189">
        <property type="reaction ID" value="UER00296"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003922">
        <property type="term" value="F:GMP synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016462">
        <property type="term" value="F:pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01742">
        <property type="entry name" value="GATase1_GMP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01997">
        <property type="entry name" value="GMP_synthase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00344">
        <property type="entry name" value="GMP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001674">
        <property type="entry name" value="GMP_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004739">
        <property type="entry name" value="GMP_synth_GATase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022955">
        <property type="entry name" value="GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025777">
        <property type="entry name" value="GMPS_ATP_PPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00958">
        <property type="entry name" value="GMP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00884">
        <property type="entry name" value="guaA_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00888">
        <property type="entry name" value="guaA_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51553">
        <property type="entry name" value="GMPS_ATP_PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0332">GMP biosynthesis</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000229446" description="GMP synthase [glutamine-hydrolyzing]">
        <location>
            <begin position="1"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1" evidence="1">
        <location>
            <begin position="24"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="GMPS ATP-PPase" evidence="1">
        <location>
            <begin position="218"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="101"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="193"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="245"/>
            <end position="251"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00344"/>
        </source>
    </evidence>
    <sequence length="535" mass="57966" checksum="AD9CC27DA44B941B" modified="2005-10-11" version="1">MTASSKPSASASDSSADGTSAHDKILIVDFGSQVTQLIARRVREEGVYSEIVPFQKAEAAFLAMKPKAVILSGGPASVLDRAAPAAPMAIFTAGVPVLGICYGEQTMAQQLGGTVEAGHHREFGRAAIEVIDACALFDGVWEKGGTYDVWMSHGDRVTKLPDGFRAVAKAPGSPIAVIADDARRFYAMQFHPEVVHTPDGAKLIRNFVRKVAGLTGDWTMRAFREEAVEKIRTQVGKGRVICGLSGGVDSSVAAILIHEAIGDQLTCVFVDHGMLRKDEGKTVVDLFRHHYNIPLIHVDASKKFLSELAGVTDPEAKRKTIGRLFIDVFEAEAKKIGGADFLAQGTLYPDVIESVSFTGGPSVTIKSHHNVGGLPDRMNMKLVEPLRELFKDEVRALGRELGLPEIFVGRHPFPGPGLAIRCPGEITNEKLDILREADAVYIDEIRKAGLYDNIWQAFAVLLPVKTVGVMGDSRTYDYVVGLRAVTSTDGMTADFYPFDAQFLGSTSTRIINEVKGVNRVVYDVTSKPPGTIEWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQR1</accession>
    <name>Q3SQR1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2125</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2125"/>
    <dbReference type="EnsemblBacteria" id="ABA05380">
        <property type="protein sequence ID" value="ABA05380"/>
        <property type="gene ID" value="Nwi_2125"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2125"/>
    <dbReference type="eggNOG" id="ENOG5032R1H">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1270291_0_0_5"/>
    <dbReference type="OMA" id="YWLARAN"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="236" mass="25580" checksum="50C1C901DA4425CD" modified="2005-10-11" version="1">MPTYTVETTYRVPVYRQRTYEAATPAEACRRAIEDDDWSNDKLDYEAAGETYVTGIWIGADAAYSGEAVPVPSHFDETIQRKAAHFEVLFGLLKIVVADQISQRGTDAYWLARANAAVAKAEAILAGARDPDEPLAPPRPFHVLAQLLEDRVRDQIAAIIETDPEFAHLTADSVADDDIRAACLAVAASIDLSEEIGAAEFRAALAALRAQEFEGRLRCTPPISASTSQSGTATAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQV4</accession>
    <name>Q3SQV4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2079</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HipA Ser/Thr kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315316.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2079"/>
    <dbReference type="EnsemblBacteria" id="ABA05337">
        <property type="protein sequence ID" value="ABA05337"/>
        <property type="gene ID" value="Nwi_2079"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2079"/>
    <dbReference type="eggNOG" id="COG3550">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041102_0_0_5"/>
    <dbReference type="OMA" id="SPAYDMN"/>
    <dbReference type="OrthoDB" id="1293681at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012893">
        <property type="entry name" value="HipA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017508">
        <property type="entry name" value="HipA_N1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13657">
        <property type="entry name" value="Couple_hipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07804">
        <property type="entry name" value="HipA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Couple_hipA" evidence="4">
        <location>
            <begin position="15"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="HipA_C" evidence="3">
        <location>
            <begin position="169"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="410" mass="45195" checksum="5E7832DB2F5AF287" modified="2005-10-11" version="1">MTESSAQVVLGESLTPIGQLRFTQTGPRQFSTFSYDPAWIKDPRAFTLQPDMPFEGGPFHASAQPGNPRDALAGAFSDAAPDSWGRRLLERAYGKGLSEFEYLTLSDDTCRQGALRFLDDQGKVITGKSVEAVPRLLDLQAITAIARAYEQGKDISAEDMQALAGAGGSGGGRPKANVRDEHVLWLAKFTSIHDQHPIEQIEVATLTLAKACGIRTPEVRLELADTPFPVALIQRFDRRGSARIPYISARTALGKTGTELGSYTEIVDFMRTAASDPKADFQELYRRLIFTILVSNKDDHLKNHGFLYVGSGRWRLSPVFDVNPAPDRNPHLETAIMEGGSHDRSIKLALDACEFFEIPEADARRTIRTAAQRISDGWRDAFRQVGVTGARVRDYEPAFINEQTDIGLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQY4</accession>
    <name>Q3SQY4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="13">TonB-dependent siderophore receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Nwi_2049</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the TonB-dependent receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315288.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2049"/>
    <dbReference type="EnsemblBacteria" id="ABA05307">
        <property type="protein sequence ID" value="ABA05307"/>
        <property type="gene ID" value="Nwi_2049"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2049"/>
    <dbReference type="eggNOG" id="COG4773">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008287_9_3_5"/>
    <dbReference type="OMA" id="RATYSHK"/>
    <dbReference type="OrthoDB" id="439303at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015344">
        <property type="term" value="F:siderophore uptake transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055072">
        <property type="term" value="P:iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012910">
        <property type="entry name" value="Plug_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037066">
        <property type="entry name" value="Plug_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011662">
        <property type="entry name" value="Secretin/TonB_short_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039423">
        <property type="entry name" value="TonB-dep_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000531">
        <property type="entry name" value="TonB-dep_rcpt_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036942">
        <property type="entry name" value="TonB_rcpt_b-brl_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010917">
        <property type="entry name" value="TonB_rcpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010105">
        <property type="entry name" value="TonB_sidphr_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32552">
        <property type="entry name" value="PTHR32552"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07715">
        <property type="entry name" value="Plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07660">
        <property type="entry name" value="STN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00593">
        <property type="entry name" value="TonB_dep_Rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00965">
        <property type="entry name" value="STN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01783">
        <property type="entry name" value="TonB-siderophor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01156">
        <property type="entry name" value="TONB_DEPENDENT_REC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0410">Iron transport</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="6 10" id="KW-0798">TonB box</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229069" evidence="11">
        <location>
            <begin position="45"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="domain" description="STN" evidence="12">
        <location>
            <begin position="77"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA05307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="833" mass="90802" checksum="F0AF1FC14458C49E" modified="2005-10-11" version="1">MRRTEQGGRGTGTVSALRNVLLGSAAALALLTNAAVLGSANAQAIPSQQGASQAIAFNIPAQDLGGALNTFADRAGLRLLFPSNLVAGRRSAGLAGTLTRNQALTRLLAGSGLTYRFTSANTVTIADPAPTPVGAAPAGAIALDTIDVDGVPSSDPGRTEGTGSYTPSVTATATRFQLTPRETPQSISVITQQQMNDFSLRSINDVVNQTPGLSTEQAGSGITNYYARGFNVQSMQFDGMQTYLNGAYPGTDTSRDMAMYDRVEVLKGTSGLTTGAGNPGATINLIRKKPTRAFQGYTTLRAGSWDDYRGELDLSGALNESGTVRGRFVTAHQDSRSYLSHREHKSSLFYGIIEADLTPDTLLTVGADYQRYVPRGYTAGMIPLFNSLGEFNTVPRSFNPGARWGTWEQNASNVFTMLEHRFDNEWRIKLHLLRQVSGYDMAVASASGGNPNPLDGSGVSLWRYNYTGKTRNDTAELFAAGPLWLFDRKHELVVGGRISRRDWRNNNFDVDPTGPGTSNIVSDFYNWTGNIPQPRLLPDPTYSNREVHNESGVYAATRLNVHDRLKVLLGARIADYEANLFNWGNRDRLKKSGQVIPYYGAVLDLTDALSAYASYTGIFMPQTAQNEQGATLPPLEGINTEFGLKAAFFDGRLNASAAYFQLKQDNYAVPSGGLTPTGTPAYRPADGVMTEGFELEISGQLSPQWQIQTGFTHNVSQLDNVRVVTLIPANQFTFYSTYKLDGALDGLTLGGGARWMDKTWENVRNPVHGTVLHTAPSYWVVDAMASYKFNDNLLGTLNIRNLLDNKYYTFYNWYNGYAWGAPRSVLLTMTYKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR73</accession>
    <name>Q3SR73_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1958</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315209.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1958"/>
    <dbReference type="EnsemblBacteria" id="ABA05218">
        <property type="protein sequence ID" value="ABA05218"/>
        <property type="gene ID" value="Nwi_1958"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1958"/>
    <dbReference type="eggNOG" id="ENOG502Z7SB">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_711479_0_0_5"/>
    <dbReference type="OMA" id="ITMPHGC"/>
    <dbReference type="OrthoDB" id="503452at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="395" mass="44967" checksum="E65B89FCBEC3858C" modified="2005-10-11" version="1">MASVTSFIRNMPASSLQAYFHHTGIELPTEVDWEAPEPEVARVTLRAVDELDDEARARIVNDAERVSALADDAGQTALYSVIDDRTVLDDLANGHARSLWMFLNEPIRFRHAEEVRYTDERRRGRSWDGFIGEPNLDLRRDEESIDAFKVALRERFASNNIHIDIFGRYRPTFDGEDCELVQIAIYREGLLDDFLAFDDAGTLVRRARRPVFEAAMTYEPATGVIEVVANDRESREEMVRFMARDLLGIEFQSEKVPFRNYDLDVLLHPFDFPTEPEDGVESVEVKQLRLMPIDNVGERVTLECLRKTDRTIWSMSAERFGANDPLAGGWVATQAKLSIKFQPKGDAKRGRTLPLTITMPHGCNLKDQTEEEQLIGEKYLRRWGILSGADGAVVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRA5</accession>
    <name>Q3SRA5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1926</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315182.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1926"/>
    <dbReference type="EnsemblBacteria" id="ABA05186">
        <property type="protein sequence ID" value="ABA05186"/>
        <property type="gene ID" value="Nwi_1926"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1926"/>
    <dbReference type="eggNOG" id="ENOG50316CX">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158569_3_1_5"/>
    <dbReference type="OMA" id="CERIWRY"/>
    <dbReference type="OrthoDB" id="1800377at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="89" mass="10298" checksum="44C0B67B51793AAD" modified="2005-10-11" version="1">MRVKQKRAIRRMRRRHQRAWLVLDQVDGRSRSECEVMDISSDGARLVIASSLSVPKRFGVALVPNATPKECERVWRNGEMMGIRFTEQG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRJ8</accession>
    <name>PYRG_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CTP synthase</fullName>
            <ecNumber evidence="1">6.3.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cytidine 5'-triphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Cytidine triphosphate synthetase</fullName>
            <shortName evidence="1">CTP synthetase</shortName>
            <shortName evidence="1">CTPS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">UTP--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrG</name>
        <name type="ordered locus">Nwi_1833</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + UTP = ADP + CTP + 2 H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:26426"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-glutamine = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + NH4(+) + UTP = ADP + CTP + 2 H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:16597"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Allosterically activated by GTP, when glutamine is the substrate; GTP has no effect on the reaction when ammonia is the substrate. The allosteric effector GTP functions by stabilizing the protein conformation that binds the tetrahedral intermediate(s) formed during glutamine hydrolysis. Inhibited by the product CTP, via allosteric rather than competitive inhibition.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">CTPSs have evolved a hybrid strategy for distinguishing between UTP and CTP. The overlapping regions of the product feedback inhibitory and substrate sites recognize a common feature in both compounds, the triphosphate moiety. To differentiate isosteric substrate and product pyrimidine rings, an additional pocket far from the expected kinase/ligase catalytic site, specifically recognizes the cytosine and ribose portions of the product inhibitor.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CTP synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315089.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRJ8"/>
    <dbReference type="SMR" id="Q3SRJ8"/>
    <dbReference type="STRING" id="323098.Nwi_1833"/>
    <dbReference type="MEROPS" id="C26.964"/>
    <dbReference type="PRIDE" id="Q3SRJ8"/>
    <dbReference type="EnsemblBacteria" id="ABA05093">
        <property type="protein sequence ID" value="ABA05093"/>
        <property type="gene ID" value="Nwi_1833"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1833"/>
    <dbReference type="eggNOG" id="COG0504">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011675_5_0_5"/>
    <dbReference type="OMA" id="EFNNAYR"/>
    <dbReference type="OrthoDB" id="783657at2"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00277"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003883">
        <property type="term" value="F:CTP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03113">
        <property type="entry name" value="CTPS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01746">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
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        <property type="entry name" value="CTP_synthase"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033828">
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    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550">
        <property type="entry name" value="PTHR11550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06418">
        <property type="entry name" value="CTP_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SSF52540"/>
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    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00337">
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    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000266163" description="CTP synthase">
        <location>
            <begin position="1"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1" evidence="1">
        <location>
            <begin position="291"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Amidoligase domain" evidence="1">
        <location>
            <begin position="1"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; for glutamine hydrolysis" evidence="1">
        <location>
            <position position="380"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="515"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="517"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="14"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
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        <location>
            <position position="71"/>
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        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="146"/>
            <end position="148"/>
        </location>
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            <name>CTP</name>
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            <note>allosteric inhibitor</note>
        </ligand>
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        <location>
            <begin position="186"/>
            <end position="191"/>
        </location>
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            <name>CTP</name>
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        <location>
            <begin position="186"/>
            <end position="191"/>
        </location>
        <ligand>
            <name>UTP</name>
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        </ligand>
    </feature>
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        <location>
            <position position="222"/>
        </location>
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            <name>CTP</name>
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        </ligand>
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            <position position="222"/>
        </location>
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        </ligand>
    </feature>
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        <location>
            <begin position="238"/>
            <end position="240"/>
        </location>
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            <name>ATP</name>
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        </ligand>
    </feature>
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        <location>
            <position position="353"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
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        <location>
            <begin position="381"/>
            <end position="384"/>
        </location>
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            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="404"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
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        <location>
            <position position="470"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01227"/>
        </source>
    </evidence>
    <sequence length="543" mass="59983" checksum="407F4D3817E1F019" modified="2005-10-11" version="1">MARYIFITGGVVSSLGKGLASAALGALLQARGYKVRLRKLDPYLNLDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTGRPATRQDNITTGRIYQDILTRERRGDYLGATIQVIPHVTNAIKAFILDGNDDHDFVLCEIGGTVGDIEGLPFFEAIRQLKNDLPRGHAIYVHLTLLPFIPSAGELKTKPTQHSVKELRSIGIQPDILLCRTDREIPAEERRKLGLFCNVRESAVIEARDADNIYAVPEVYHAAGLDDEVLAAFGIEPAAPPALKGWHDINERIRNPEGAVTIAIVGKYTGMKDAYKSLIEALSHGGIANKVKVNLDWIESEVFENEDAAPFLEHVDGILVPGGFGQRGAEGKIKAARFARERHVPYFGICFGMQMAVIEAARNLVGISDANSTEFGPTREPLVGLMTEWLRGSELEKRSKAGDLGGTMRLGAYPAMLKRGSRVSGIYGGAVEISERHRHRYEVNTAYKDRLEQHGLRFSGLSPDGVLPEIVEYQDHPWFIGVQYHPELKSRPFEPHPLFASFIQAAVVQSRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRK6</accession>
    <name>Q3SRK6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PE_PGRS family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1824</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315081.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05085">
        <property type="protein sequence ID" value="ABA05085"/>
        <property type="gene ID" value="Nwi_1824"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1824"/>
    <dbReference type="HOGENOM" id="CLU_857457_0_0_5"/>
    <dbReference type="OMA" id="AGSWRMA"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229169" evidence="2">
        <location>
            <begin position="32"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="324" mass="29235" checksum="199DE333020146F5" modified="2005-10-11" version="1">MPRSASAARAAFLSTALLTLAVAADSAPAFAQSFGGDGGTAAGTPAAAGGGFGIAEENGTVANGGAGGGGGAGAAGGNSVTGQSGGTAGLDADQADGGQGGDGNMTATPGVAGSGGGGGNGFIAAGGSVAITTGFRGGHGGEGGRSLGEHGGGGGAGGYGVVDLIGTAFLFDLGSGTLQGGNGGDGGDGVYIGAPGAALTIVSGTIEGGGGGYGGRFAILNHNAAYTAGSGGDGGAALLLHAGASLSNHGVIRGGDGGHGAPGVMRINSTEGDGGDGGAGIVANATSIINTGTISGGNGGVRGWDVLVPSAKQEPAELASPDRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRS8</accession>
    <name>Q3SRS8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Biotin carboxyl carrier protein of acetyl-CoA carboxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_1752</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 5">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315009.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1752"/>
    <dbReference type="EnsemblBacteria" id="ABA05013">
        <property type="protein sequence ID" value="ABA05013"/>
        <property type="gene ID" value="Nwi_1752"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1752"/>
    <dbReference type="eggNOG" id="COG0511">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016733_3_1_5"/>
    <dbReference type="OMA" id="TAYWAPE"/>
    <dbReference type="OrthoDB" id="1938042at2"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009317">
        <property type="term" value="C:acetyl-CoA carboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001249">
        <property type="entry name" value="AcCoA_biotinCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01071">
        <property type="entry name" value="ACOABIOTINCC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00531">
        <property type="entry name" value="BCCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0092">Biotin</keyword>
    <keyword evidence="5" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="7">
        <location>
            <begin position="85"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA05013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="161" mass="17036" checksum="40444F7F61D0163D" modified="2005-10-11" version="1">MSRQPGEPAKNKTGNDLREESDVIRELAALLDETRLTEIEIERDGLRVRVARNVTVGAAIPATYQAAPAPAPSPAAGAADISKHPGMVPSPMVGTAYWASEPGAKPFIEVGSKVTAGQTLLIIEAMKTMNQIPSPRSGTVTQILVEDGQPVEFGEPLVIIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS20</accession>
    <name>Q3SS20_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FeS_assembly_P domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1660</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314920.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1660"/>
    <dbReference type="EnsemblBacteria" id="ABA04921">
        <property type="protein sequence ID" value="ABA04921"/>
        <property type="gene ID" value="Nwi_1660"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1660"/>
    <dbReference type="eggNOG" id="COG2151">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091588_1_2_5"/>
    <dbReference type="OMA" id="PPWGPER"/>
    <dbReference type="OrthoDB" id="1668114at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="SSF117916"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FeS_assembly_P" evidence="1">
        <location>
            <begin position="33"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="126" mass="13719" checksum="A1AC8DE4F4F12D20" modified="2005-10-11" version="1">MSDTAELVVNKTCHMQTSSALSAEETERMGAGIVAALKTVFDPEIPADIYELGLIYKVDIKDDRAVDVEMTLTTPNCPSAAELPIMVENAVASVPGVGVVNVNIVWEPQWVPDRMTDEARAVLNMW</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS55</accession>
    <name>Q3SS55_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phage major capsid protein, HK97</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1482</name>
        <name evidence="2" type="ordered locus">Nwi_1625</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="2">Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1482"/>
    <dbReference type="EnsemblBacteria" id="ABA04743">
        <property type="protein sequence ID" value="ABA04743"/>
        <property type="gene ID" value="Nwi_1482"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04886">
        <property type="protein sequence ID" value="ABA04886"/>
        <property type="gene ID" value="Nwi_1625"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1482"/>
    <dbReference type="KEGG" id="nwi:Nwi_1625"/>
    <dbReference type="eggNOG" id="COG4653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041417_0_0_5"/>
    <dbReference type="OMA" id="FHRFDCV"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024455">
        <property type="entry name" value="Phage_capsid"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05065">
        <property type="entry name" value="Phage_capsid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01554">
        <property type="entry name" value="major_cap_HK97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="385" mass="41138" checksum="7D87569DDD6E6C90" modified="2005-10-11" version="1">MGKHFSDLEFKDTDDADPVDLVTKAVNDLTTNVDARLKDIETKADTTKLVDRMDKLEAKINRPGSGDHKDPDAALEIKAFGAYVRSGITPPDPLELKTLIVSSDPQGGYLAPTEMATEFIRDLVQYSPIRGLASVRSTSAPAVSYPKRIGRTNAQWRGETQAQTTGEPTFGQLEIPVREINTYVDISNQLLADSAGAAESEVRMALAEDFGLKEGLSFLKGTGPLQPEGLLINADISIVATGNASTLGSGPADMLIDTFYSLPAAYRNAGTWLMNSTTLAAIRKLKDGTTGTYLWSPGFQGQADTILGRPVIDCPDMDDVGSGTTPIAFGDIAATYRILDRIGLSILANPYLLATTGTTRIHATRRVGGAVVQPAAMKKIVCKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS64</accession>
    <name>Q3SS64_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phage Terminase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1616</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314881.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1616"/>
    <dbReference type="EnsemblBacteria" id="ABA04877">
        <property type="protein sequence ID" value="ABA04877"/>
        <property type="gene ID" value="Nwi_1616"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1616"/>
    <dbReference type="eggNOG" id="COG4626">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026632_6_0_5"/>
    <dbReference type="OMA" id="FGEKWYF"/>
    <dbReference type="OrthoDB" id="1527109at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005021">
        <property type="entry name" value="Terminase_largesu_Lambdalike"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41287">
        <property type="entry name" value="PTHR41287"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03354">
        <property type="entry name" value="Terminase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="537" mass="60450" checksum="BD679DBCB0B812FD" modified="2005-10-11" version="1">MLAPAWINDGSDIPDPFGYGERAVQWLRRLKHPKNPARGHPFQVDPWQERIIRKLYGPRHEDGRRVVRRLVLLLPRGNRKTSLCGAITLLHLIGPEKLPGNLVVSAASAHEQAMELFNEVAMIVQHDRRLEKHLAVREYTSSITFKAQRSRYIAVASDGKVQHGKTPNVVIADELHAWEGRAGRVQWEALDSALVKVPDTLMIVATTSGRGQENLAWKTIDYAIKVQKGEIDDPATLPVVFMAEEGDDWRDEAVWHALNPGMRHGYPDLTSYRDKAQKAINSPSDRDSFLQFNLNRWLDHSTSPFVEMLVYDQGARAIDLDELKGEQCWIGVDLSSNSDLTAVVACWRDGDDGYQVQPWFFCPEDNLRRRADKDGVAYPAWADDGFIIPTPGNVVDFHAVENHIRDLCERFNVREIAFDPHLARNMLSNLLADGLPAVEMRQGWVTMAPAIKELERAIVGRRFRHGGHPVLRWNFDNIATETDKAGNKSFHKGKSRDRIDGAVATAMAVARCAAGDNARSIYDSPNWSPDWAFVKAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS66</accession>
    <name>Q3SS66_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1614</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314879.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1614"/>
    <dbReference type="EnsemblBacteria" id="ABA04875">
        <property type="protein sequence ID" value="ABA04875"/>
        <property type="gene ID" value="Nwi_1614"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1614"/>
    <dbReference type="eggNOG" id="ENOG50339EY">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2001458_0_0_5"/>
    <dbReference type="OMA" id="HAYNERR"/>
    <dbReference type="OrthoDB" id="2001265at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="131" mass="14889" checksum="9AF9DB954288F972" modified="2005-10-11" version="1">MINLQWLSDRLVYILRWRMDRHPGAENEPDAPQIPVAGLRVWGIFLDLHATRGSNGFGANPIAYADIEAWSRLRREPVRPFELDMIRALDTTWLEVAAAMAKEPDKPKVSSRKVTPALFDAMFGEGKRGME</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS84</accession>
    <name>Q3SS84_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="13">CDP-alcohol phosphatidyltransferase</fullName>
            <ecNumber evidence="13">2.7.8.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Nwi_1596</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="11">Phospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.8.5" evidence="13"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314861.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1596"/>
    <dbReference type="EnsemblBacteria" id="ABA04857">
        <property type="protein sequence ID" value="ABA04857"/>
        <property type="gene ID" value="Nwi_1596"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1596"/>
    <dbReference type="eggNOG" id="COG0558">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051314_6_2_5"/>
    <dbReference type="OMA" id="KRFNMAS"/>
    <dbReference type="OrthoDB" id="1702107at2"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008444">
        <property type="term" value="F:CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004570">
        <property type="entry name" value="Phosphatidylglycerol_P_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000847">
        <property type="entry name" value="Phos_ph_gly_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA04857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="180" mass="19349" checksum="F5D1075C5B4AEEC1" modified="2005-10-11" version="1">MSIPNIITLGRILLVPIIIWAIASNQMEIAFAIFVIAGVSDAVDGFLAKRFNMASELGALLDPLADKALLVSIFVTLGIWGAVPIWLVILVVSRDIMIVGAVIVSWLFNKPVPMKPLLVSKLNTGAQVAFAALVLAALGFGFDSSPYEALLMAAIAILTLVSVSFYLVEWVRHMSTIDPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSB1</accession>
    <name>Q3SSB1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lectin-like protein BA14k</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_1569</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Has immunoglobulin-binding and hemagglutination properties, and can bind to mannose. Essential for virulence. May be involved in LPS biosynthesis or polysaccharide transport.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the BA14k family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04830">
        <property type="protein sequence ID" value="ABA04830"/>
        <property type="gene ID" value="Nwi_1569"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1569"/>
    <dbReference type="eggNOG" id="ENOG5033457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092318_0_0_5"/>
    <dbReference type="OMA" id="PSYCARR"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012413">
        <property type="entry name" value="BA14K"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07886">
        <property type="entry name" value="BA14K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0430">Lectin</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0843">Virulence</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229097" description="Lectin-like protein BA14k" evidence="8">
        <location>
            <begin position="27"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA04830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="211" mass="22282" checksum="23828E00196F8C15" modified="2005-10-11" version="1">MFRFKSVLAATVIALAPLVVASPSFAQGWGSKAMGFSGGGARIGGGSFMGGGRHFGGGGRHFGGGWRGGGRMGWAGRGWGGHRIGWAGRGWRGHRIGWGGRGWRRGWGGGWGGGFYPGLIGGGLLGGGLWGSPWGWGWGGGWGPGLGWGSPFYSDYGYDDGYYDDGPAVAPTVVRTNGRDVTYCQRRFKSYDVRSGTYLGRDGRRHACPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSC8</accession>
    <name>Q3SSC8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1552</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314819.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1552"/>
    <dbReference type="EnsemblBacteria" id="ABA04813">
        <property type="protein sequence ID" value="ABA04813"/>
        <property type="gene ID" value="Nwi_1552"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1552"/>
    <dbReference type="HOGENOM" id="CLU_1137107_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="244" mass="27361" checksum="E83909068C5C3BC1" modified="2005-10-11" version="1">MTVSADEEHISLTEAIALSLQDYWNSIGLYLPHCRAEDIARASLLRLQPRRTALRDLRELDGLGLAEETRVRELAFQDAVAELARRSATDGDQDWSECKVAAMLAQKRDDIYYCDSSCRIDELPPPLFRMGYEWAALSIIRILEGVLAKPSEDICATTEFQHIGKGLYKQVGEPRPSDPRRRAALDLIKEIERYGQNALRGMFFRSKRKNAYAPADDAALAELIKEKRETLSGGLFQCARGGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSE7</accession>
    <name>Q3SSE7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase, family 39</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1533</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1533"/>
    <dbReference type="CAZy" id="GT83">
        <property type="family name" value="Glycosyltransferase Family 83"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04794">
        <property type="protein sequence ID" value="ABA04794"/>
        <property type="gene ID" value="Nwi_1533"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1533"/>
    <dbReference type="eggNOG" id="COG1807">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019200_1_0_5"/>
    <dbReference type="OMA" id="GHSAPWY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="127"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="157"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="181"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="219"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="311"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="342"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="366"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="395"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="423"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="455"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="584" mass="64227" checksum="AC6EFA9CA18B15DC" modified="2005-10-11" version="1">MRNPMAEPLSSPRFGRPREPKNPLPMGRGLAMVIDFVVVSHIRSALFLTVFGLLFFLPGFFNIPPIDRDEARFAQATKQMVETGDFVDIRFQGEVRYKKPVGIYWLQAAAVETASALGLPRAQIRIWLYRVPSLIGAIGAVLLTYWAALAFITRRGAILAGLIMCGSVLLGVEARLAKTDAMLLLTIVAAMGALARIYLSSRRGENPEHPSWTHPAIFWTALAGGILLKGPLILMFVGLTIGAVAILDRSASWLWRLRSVWGLMWVLVLVTPWFVAIFLRAGDTFFTDSIGGDMLSKLAAQESHGAPPGTYFLLFWITFWPGAALAGLAAPAVWRARREPEVRFLLAWLIPSWIVFELVLTKLPHYVLPLYPAIAILSVWALERRVLSRSWLTKGTAWWFAIPAITLVLAIVATITLTRQPVFLAWPFAAVALISGLFAWWLYDDDNAERSLLNAVLAALFLSASVYGIIVAALTPLFPSPEVARALRNVECVGPKAAAAGYHEPSLVFMTGTSTLLTDGSGAADFLLHGSCRFALVESRSERAFAQRAETIGLRYNVATRIDGFNFSQGKTISIAIFRSEGTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSF4</accession>
    <name>Q3SSF4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Amino acid ABC transporter ATP-binding protein, PAAT family</fullName>
            <ecNumber evidence="7">3.6.3.27</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_1526</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in beta-(1--&gt;2)glucan export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.27" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314793.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1526"/>
    <dbReference type="EnsemblBacteria" id="ABA04787">
        <property type="protein sequence ID" value="ABA04787"/>
        <property type="gene ID" value="Nwi_1526"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1526"/>
    <dbReference type="eggNOG" id="COG1126">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_5"/>
    <dbReference type="OMA" id="NKYPGQM"/>
    <dbReference type="OrthoDB" id="1191583at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015424">
        <property type="term" value="F:ABC-type amino acid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030679">
        <property type="entry name" value="ABC_ATPase_HisP-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039085">
        <property type="entry name" value="ABC_ATPase_HisP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="8"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="40"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="247" mass="27685" checksum="3BDFF24E3603BE4E" modified="2005-10-11" version="1">MTDNNAIVSIIALNKWFGSFHVLRDIDLDVARGERIVICGPSGSGKSTLIRCINALEEFQEGRIVVDGIELGPNLRRVDDVRREVGMVFQNFNLFPHLTVLENCTLAPIWVRNTPKKDAEAAAMKFLERVKIPDQANKYPGQMSGGQQQRVAIARALTMNPKVMLFDEPTSALDPEMVKEVLDTMVDLAEEGMTMLVVTHEMGFAREVANRVVFMDEGQIIESDTPASFFANPRHPRSQLFLSQILR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSG8</accession>
    <name>Q3SSG8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1512</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314779.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1512"/>
    <dbReference type="EnsemblBacteria" id="ABA04773">
        <property type="protein sequence ID" value="ABA04773"/>
        <property type="gene ID" value="Nwi_1512"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1512"/>
    <dbReference type="eggNOG" id="COG3706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_663307_0_0_5"/>
    <dbReference type="OMA" id="MRKMDFG"/>
    <dbReference type="OrthoDB" id="717845at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="409" mass="44201" checksum="D4BFF9E773C52329" modified="2005-10-11" version="1">MSQQGPLIVVSHGERQASVVSRASRSIFPVLDIDWSEASRGIERFQPAAVFVPTTEGAGVGLDRLAGQLAAMQPYVPLIVADPPMPCPDNALPFSSLNEGIDRFDRRLNAALRVRTLHATVLRRLAGNPAAPSLPDADPIRDATVLLIGRGAAYPTLSVAFGERLGVVGALSIEAAAKHLNARDLDGVVISEGFARRVVDALLTVLAEDTRFRNLPIAVPAGGPWATYDLPNLEVMPSEPHHAVSTFLPLVRQHAFEARLHRTLKSMDAGGLLDPRTGLLTGDAFHRELNMAIRQTRSSGASLSIARFSLGQQHERPLFDAARILSRLMRRMDFGTLEADGSIVVVFADADLRNAQMIARRLSSVMKHTTLSAKRTVRIDPQVTLTASRFDDSAVSLLTRLSSEARAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSH1</accession>
    <name>Q3SSH1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Invasion associated locus B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1509</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314776.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1509"/>
    <dbReference type="EnsemblBacteria" id="ABA04770">
        <property type="protein sequence ID" value="ABA04770"/>
        <property type="gene ID" value="Nwi_1509"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1509"/>
    <dbReference type="eggNOG" id="COG5342">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080086_0_0_5"/>
    <dbReference type="OMA" id="TCSKQED"/>
    <dbReference type="OrthoDB" id="1409693at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038696">
        <property type="entry name" value="IalB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010642">
        <property type="entry name" value="Invasion_prot_B"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06776">
        <property type="entry name" value="IalB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229252" evidence="2">
        <location>
            <begin position="22"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="244" mass="26101" checksum="3ADDBC65B67BBBDE" modified="2005-10-11" version="1">MNFRLLTTSALHRGRAFALFAAVTLVAVPFASDAPAQQAPPPKGKAAPAKGAQQAPAANQAAPADQQVQLIYAPWTKFCLKGQDAKAKQVCFTGKDGRIESGQPVIAAVIIEPEGEPKKILRVTLPLGMQLVHGTRIIVDSNPPQQSPYVICFANGCMSDYELSADMLGHLKKGQNLIVQAINSNGAPLTLPLPLAEFAKAYDGPPTDPKVFEETQKKLQEELQKRAAEARAKLEATQQGTAKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSM6</accession>
    <name>Q3SSM6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1454</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1454"/>
    <dbReference type="EnsemblBacteria" id="ABA04715">
        <property type="protein sequence ID" value="ABA04715"/>
        <property type="gene ID" value="Nwi_1454"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1454"/>
    <dbReference type="eggNOG" id="ENOG502ZYAZ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139742_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="160" mass="17366" checksum="71A7965A6A0B7136" modified="2005-10-11" version="1">MDTVRAKKTRQNSSWRASLLIQSEAERLQTDECRLETDALLILTANDVRTCCLRALRSRVAVVFLIVTYLLSGASHHCFDVNVSAPESRIVLSMASLHADTDSPGALADHHCHGCFQVSLPDPPLFSVAIEHQAAAVPRAMTRASDLVPGIDTPPPKLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSM9</accession>
    <name>Q3SSM9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Succinate semialdehyde dehydrogenase</fullName>
            <ecNumber evidence="4">1.2.1.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1451</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.1.16" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314719.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1451"/>
    <dbReference type="EnsemblBacteria" id="ABA04712">
        <property type="protein sequence ID" value="ABA04712"/>
        <property type="gene ID" value="Nwi_1451"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1451"/>
    <dbReference type="eggNOG" id="COG1012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_1_0_5"/>
    <dbReference type="OMA" id="LIAHPVI"/>
    <dbReference type="OrthoDB" id="384611at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009013">
        <property type="term" value="F:succinate-semialdehyde dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="3">
        <location>
            <begin position="14"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="478" mass="50760" checksum="32A9C2E49C1B7760" modified="2005-10-11" version="1">MSYQDTSLLIDGRWRPGASGKTIAVLNPATEEQIGTVAHADRADLDEALAAADKGFKIWRAVAPFERARIMRKAAALLRERVERIATLMTMEQGKVLAEARIETLAAADVIEWFAEESRRAYGRVIPARGPGIYQIAIKEPVGPAAAFTPWNFPINQVCRKLSAALAAGCSIIVKAPEETPASPAELLRVFIDAGVPDGVINLVFGVPAEISEYLIPHPIIRKISFTGSTAVGKQLAALAGLHMKRATMELGGHAPAVVFDDADIASAVKIISDAKFRNAGQVCIAPTRLLVQEKVYKDFVGKFTEAAKVVRVGNGLDPDSTMGSLANSRRVSAVEGFVQDAIGKGAKLAAGGHRVGNKGYFFEPTVLVDVPKEARAMNEEPFGPLALISPFSSFDEAAEESNRLPYGLASYAFTSSTRTATAIGAAIESGMVAINNVALALPENPFGGVKDSGYGSEGGIEAMESYLITKFISQTGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SST1</accession>
    <name>Q3SST1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Flg_hook domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1399</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314669.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1399"/>
    <dbReference type="EnsemblBacteria" id="ABA04660">
        <property type="protein sequence ID" value="ABA04660"/>
        <property type="gene ID" value="Nwi_1399"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1399"/>
    <dbReference type="eggNOG" id="ENOG5033C35">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030873_0_0_5"/>
    <dbReference type="OMA" id="RMWAERP"/>
    <dbReference type="OrthoDB" id="697236at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021136">
        <property type="entry name" value="Flagellar_hook_control-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038610">
        <property type="entry name" value="FliK-like_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02120">
        <property type="entry name" value="Flg_hook"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flg_hook" evidence="2">
        <location>
            <begin position="463"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="321"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="529"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="243"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="550" mass="55857" checksum="D253289D6FCF48BA" modified="2005-10-11" version="1">MAISINPIFPVIAAQDAASEAVLQPGRVVDAKVQGILADNLVRIAIANLTIEALSEIPVQVGQSLRLAVSQTGQGIRLAVVGAGSSGTLAAGPADALTLTPDIALAMTSARAAPAPVLTALEALSVSVAAQAAAARQGSLSPLYANMLVAAGSGSLPPDVRQALHRLLALRPGLDPGLSGSDIKTAFRTSGLFLEASLAAGLETGQPAASANRQLPDMKAALLVLREALSISVGDVSRAAPPAASAGSQQTASPIATGAPPLSPDIDAQDVYLLQARPAGIDDPDLPANQTLFPRASDAGLRAAVTGAALNALQEVLQNRGNNGLGAESGQGGDPDAAPPLRTGPASGPAGDNVFIVRTSTPPPPFGGALPAAQPVALPGLSPAASPEEIARRLLDDTGAAISRQVLLQIASLPDRADAMGTRFDQMMPRWNFEIPFVVPAGTAVAQFEISRDAAGHDTETVTRVWRARFSIDVEPAGPVHALVSLTGDTAAVRLWAERPSTASRLRAGASQLTQALSRAELQPGDIIIGEGAPPQPIPAPAGHFLDRAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSX5</accession>
    <name>Q3SSX5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acetyltransf_6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1355</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314634.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1355"/>
    <dbReference type="EnsemblBacteria" id="ABA04616">
        <property type="protein sequence ID" value="ABA04616"/>
        <property type="gene ID" value="Nwi_1355"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1355"/>
    <dbReference type="eggNOG" id="COG5653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051159_0_0_5"/>
    <dbReference type="OMA" id="ICANQIA"/>
    <dbReference type="OrthoDB" id="1856290at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038740">
        <property type="entry name" value="BioF2-like_GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13480">
        <property type="entry name" value="Acetyltransf_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyltransf_6" evidence="1">
        <location>
            <begin position="169"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="372" mass="41011" checksum="E608A144665814FB" modified="2005-10-11" version="1">MISVNICPPTPDLAAPWDDLVRRASSNVFMNPVVLSAANESGFADVRVLLAWNDGAGFKKLVGIWAFQVRKISPMWPLVLEALPYNYAFLSSPVVDPAFVRDVIPGFLSAIRDSRSLPKVISLKSLDAEEESFATLIETLNEQGCACLRMSDSTRPFATPDAGVKRSGSTRKKLRQDWNRLSAAGSVDIVNLRAPDTREAFETFLELEAAGWKGARGTALLCRPADAAFARKMIAALAERGDASVALLRVDGRAIAAQVLMYCGATAYTWKTAFDPDYAKYSPGMLLIDRITDDLLTSADIEAVNSCSYEGSFMAQLWVGRRRMADLLVNVGRDRSLAFSLEAARQRGYEQLRWFRDQVRSWSVPASPKARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSX6</accession>
    <name>Q3SSX6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAD_binding_3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1354</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1354"/>
    <dbReference type="EnsemblBacteria" id="ABA04615">
        <property type="protein sequence ID" value="ABA04615"/>
        <property type="gene ID" value="Nwi_1354"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1354"/>
    <dbReference type="eggNOG" id="COG0654">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2194170_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_3" evidence="1">
        <location>
            <begin position="37"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="108" mass="11327" checksum="DF2F2368741869A2" modified="2005-10-11" version="1">MVKPHPVEPVALLAPSRFVGFCGALINLANKGGSMRYTNIAIVGGGLAGSTAAAMLGRAGVPAILMDPHTTYLPDLRCEKLSGTQIRRLRNTGLDKQILQATTLDGEV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-17" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST24</accession>
    <name>PURQ_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribosylformylglycinamidine synthase subunit PurQ</fullName>
            <shortName evidence="1">FGAM synthase</shortName>
            <ecNumber evidence="1">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Formylglycinamide ribonucleotide amidotransferase subunit I</fullName>
            <shortName evidence="1">FGAR amidotransferase I</shortName>
            <shortName evidence="1">FGAR-AT I</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glutaminase PurQ</fullName>
            <ecNumber evidence="1">3.5.1.2</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphoribosylformylglycinamidine synthase subunit I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purQ</name>
        <name type="ordered locus">Nwi_1306</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ADP + H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-glutamine = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="1"/>
    <dbReference type="EC" id="3.5.1.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314587.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ST24"/>
    <dbReference type="SMR" id="Q3ST24"/>
    <dbReference type="STRING" id="323098.Nwi_1306"/>
    <dbReference type="EnsemblBacteria" id="ABA04567">
        <property type="protein sequence ID" value="ABA04567"/>
        <property type="gene ID" value="Nwi_1306"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1306"/>
    <dbReference type="eggNOG" id="COG0047">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001031_3_1_5"/>
    <dbReference type="OMA" id="LHFVCRD"/>
    <dbReference type="OrthoDB" id="1527083at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00421">
        <property type="entry name" value="PurQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010075">
        <property type="entry name" value="PRibForGlyAmidine_synth_PurQ"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001586">
        <property type="entry name" value="FGAM_synth_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01737">
        <property type="entry name" value="FGAM_synth_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000252714" description="Phosphoribosylformylglycinamidine synthase subunit PurQ">
        <location>
            <begin position="1"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1" evidence="1">
        <location>
            <begin position="3"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00421"/>
        </source>
    </evidence>
    <sequence length="233" mass="25000" checksum="1254B22E8F39B9B3" modified="2005-10-11" version="1">MKSAVLVFPGINRERDMARALRLVSGREPAMVWHAETSLPEGTDLVVAPGGFSYGDYLRCGAIAARAPVMDAVRAFASDGGLVLGVCNGFQILCESGLLPGILMRNARLKFICHDVHLRVERSDTPFTRGYNAGQVIRVPVAHGEGNYAADEDTIRRLEGEGRVLYRYCSASGEVGDTHNINGAAQSIAGIVNERGNVLGMMPHPENHVEDIMGCTDGRGLFAGLVAHLERAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST36</accession>
    <name>Q3ST36_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Isocitrate dehydrogenase [NADP]</fullName>
            <ecNumber evidence="8">1.1.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Nwi_1294</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>D-threo-isocitrate + NADP(+) = 2-oxoglutarate + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:19629"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8 11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="8 11">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="8 11">Binds 1 Mg(2+) or Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.42" evidence="8"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314578.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1294"/>
    <dbReference type="EnsemblBacteria" id="ABA04555">
        <property type="protein sequence ID" value="ABA04555"/>
        <property type="gene ID" value="Nwi_1294"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1294"/>
    <dbReference type="eggNOG" id="COG0538">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023296_1_1_5"/>
    <dbReference type="OMA" id="HGTVQRH"/>
    <dbReference type="OrthoDB" id="1551125at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004450">
        <property type="term" value="F:isocitrate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004790">
        <property type="entry name" value="Isocitrate_DH_NADP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11822">
        <property type="entry name" value="PTHR11822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000108">
        <property type="entry name" value="IDH_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00127">
        <property type="entry name" value="nadp_idh_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 8 11" id="KW-0464">Manganese</keyword>
    <keyword evidence="3 8 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8 12" id="KW-0521">NADP</keyword>
    <keyword evidence="6 8 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Iso_dh" evidence="13">
        <location>
            <begin position="9"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="94"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="75"/>
            <end position="77"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="308"/>
            <end position="313"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="9">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="9">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABA04555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="403" mass="45108" checksum="7885DA17BE0EA51D" modified="2005-10-11" version="1">MAKIKVTNPVVELDGDEMTRIIWQYIKDKLIHPFLDIDLMYFDLGMESRDETDDRITVEAANAIRKVGVGVKCATITPDEARVKEFGLKHMWKSPNGTIRNILGGVIFREPIICKNVPRLVPGWTKPIIIGRHAYGDQYRATDIRFPGKGTLSMKFVGEDGAVIEREVFKTPGPGVAMGMFNIDDSIIDFARASFNYGLQRGFPVYLSTKNTILKVYDGRFKDIFQDIYDREFRKDFEAKGLTYEHRLIDDMVASALKWSGGYVWACKNYDGDVQSDTVAQGYGSLGLMTSVLLTPDGQTVEAEAAHGTVTRHYREHQKGKETSTNSIASIFAWTRGLSHRAKLDDNEALAKFATTLETVCVDTVEAGFMTKDLALLVGPDQRWLSTTGFLDKVADNLTRTMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST77</accession>
    <name>Q3ST77_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Type I restriction enzyme EcoAI specificity protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_1253</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-I restriction system S methylase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1253"/>
    <dbReference type="REBASE" id="11318">
        <property type="entry name" value="S.NwiORF1254P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04514">
        <property type="protein sequence ID" value="ABA04514"/>
        <property type="gene ID" value="Nwi_1253"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1253"/>
    <dbReference type="eggNOG" id="COG0732">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021095_10_4_5"/>
    <dbReference type="OMA" id="LMARCDE"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.220.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000055">
        <property type="entry name" value="Restrct_endonuc_typeI_TRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044946">
        <property type="entry name" value="Restrct_endonuc_typeI_TRD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01420">
        <property type="entry name" value="Methylase_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0680">Restriction system</keyword>
    <feature type="domain" description="Methylase_S" evidence="4">
        <location>
            <begin position="421"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="597" mass="65910" checksum="A6802C72A1CE3D07" modified="2005-10-11" version="1">MNDGPMNAERLLSAYEKIADAPDAIARLRRFVLDLAVRGKLVPQDANDEPASELLKRIAKEKARLVKAGEIRKPKAIPALPEPPFPIPSNWRWSQLAEIGVLSPRNEAPDTLEASFVPMPLIAAEYGVANQHEIRPWGEIKKGYTHFAEGDVGLAKITPCFENGKSTVFRNLTGGIGTGTTELHIVRPLFVDQDYILLFLKSPHFIETGIPRMTGTAGQKRVPTEYFAHSPFPLPPLAEQHRIVAKVDALMGLCDRLKTAREQRETVRDRLAAASLARLNAPDPEPGADPAKVGTGFASGSATNEKFQADARFALDALPALTTRPDQIKALRQTILNLAVRGKLVPQDPNDEPASELLKRIARAKAERKKKTGDARIKIEPDPTPDELNMPIPVEWAVQSFENLFLFIDYRGNTPPKTDEGIPLITAKNIRMGYLNREPREFISKATFKTWMTRGFPEIGDLFFTTEAPLANVCLNDIEEPFALAQRAICFQPYAKIDTKFLMFALMSDVMQSLIDKHATGMTAKGIKAAKLKPLPIPIPPLAEQHRIVAKVDELMALCDRLEASLTATAATSRRLLDALLAEALAPAEDREMEAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STB6</accession>
    <name>Q3STB6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">6-phosphogluconate dehydrogenase, NAD-binding protein</fullName>
            <ecNumber evidence="5">1.1.1.31</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_1213</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.31" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314504.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1213"/>
    <dbReference type="EnsemblBacteria" id="ABA04475">
        <property type="protein sequence ID" value="ABA04475"/>
        <property type="gene ID" value="Nwi_1213"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1213"/>
    <dbReference type="eggNOG" id="COG2084">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035117_1_0_5"/>
    <dbReference type="OMA" id="IFVDHTT"/>
    <dbReference type="OrthoDB" id="1194694at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008442">
        <property type="term" value="F:3-hydroxyisobutyrate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016054">
        <property type="term" value="P:organic acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002204">
        <property type="entry name" value="3-OH-isobutyrate_DH-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015815">
        <property type="entry name" value="HIBADH-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029154">
        <property type="entry name" value="NADP-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14833">
        <property type="entry name" value="NAD_binding_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000103">
        <property type="entry name" value="HIBADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00895">
        <property type="entry name" value="3_HYDROXYISOBUT_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD_binding_2" evidence="3">
        <location>
            <begin position="3"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="NAD_binding_11" evidence="4">
        <location>
            <begin position="167"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000103-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="289" mass="30360" checksum="E4F55981DAE75ADC" modified="2005-10-11" version="1">MAKVAFLGLGVMGFPMAGHLVAKGGHEVTVFNRTPAKADAWAEKFGGRTAATPESAAEGQDFVMACVGNDDDLRAVTIGAGGAFAGMKPGAVFVDHTTASAQVARELDAEATRRGFGFVDAPVSGGQAGAENGLLTVMCGGAEDAYTRAEPIVAAYARMCKRLGPAGAGQLTKMVNQICIAGLIEGLSEGIHFAKKAGLDVNAVVEVISKGAAQSWQMENRFKTMSEGRFDFGFAVEWMRKDLSICISEARRNGASLPVTSLVDAFYAEVEKMGGPRWDTSSLLARLER</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="126" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STD1</accession>
    <name>Q3STD1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_1198</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314489.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1198"/>
    <dbReference type="EnsemblBacteria" id="ABA04460">
        <property type="protein sequence ID" value="ABA04460"/>
        <property type="gene ID" value="Nwi_1198"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1198"/>
    <dbReference type="eggNOG" id="COG0642">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_6_5"/>
    <dbReference type="OMA" id="DASKKIM"/>
    <dbReference type="OrthoDB" id="692375at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="9">
        <location>
            <begin position="183"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="244"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="214"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA04460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="486" mass="52245" checksum="9B0A6358B4982E99" modified="2005-10-11" version="1">MTAFGKLFRTTAFRLTLVYLLLFALFAASLLGYFAWNTRRLITDQIAATVGEEIGLLTGVFNRRGLRGLVGAIENRALRPGSNLYLVTTPAGLGVAGNVGSLQPGVMETAGWAETAYRRLDEQDQAHHYALVRVTELSNGFRLLIGRDLEERHRIIGVVASAAQWSLLIVVVLGLGGGVFVARRVLHRIDAMTGTTKRIMAGDLSGRLPVGRSGDELDRLAENLNAMLERIEALMNGLREVSDNIAHDLKTPLTRLRNRAEEALARSGNESDYRAALEQTIEESDGLIRTFNALLMIARAESGHARDNMADFDAADVAEGIHELYEPLAEDNGMKLQVQTGFAPLHGNRELISQALANLVENAIKYGQPKPASPPLGADAVAADATILIEARHEGDRIILAVTDHGSGIPEADRKRAVERFVRLETSRTLAGSGLGLSLASAVARLHGGELALDDAQPGLRATLSIPARSASGLAAPTVDVQQNDA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STL0</accession>
    <name>FLGH_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Flagellar L-ring protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Basal body L-ring protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">flgH</name>
        <name type="ordered locus">Nwi_1119</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Assembles around the rod to form the L-ring and probably protects the motor/basal body from shearing forces during rotation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The basal body constitutes a major portion of the flagellar organelle and consists of four rings (L,P,S, and M) mounted on a central rod.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell outer membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Bacterial flagellum basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FlgH family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314412.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3STL0"/>
    <dbReference type="SMR" id="Q3STL0"/>
    <dbReference type="STRING" id="323098.Nwi_1119"/>
    <dbReference type="EnsemblBacteria" id="ABA04381">
        <property type="protein sequence ID" value="ABA04381"/>
        <property type="gene ID" value="Nwi_1119"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1119"/>
    <dbReference type="eggNOG" id="COG2063">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069313_1_2_5"/>
    <dbReference type="OMA" id="QARISYG"/>
    <dbReference type="OrthoDB" id="1900876at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009427">
        <property type="term" value="C:bacterial-type flagellum basal body, distal rod, L ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00415">
        <property type="entry name" value="FlgH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000527">
        <property type="entry name" value="Flag_Lring"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34933">
        <property type="entry name" value="PTHR34933"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02107">
        <property type="entry name" value="FlgH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01008">
        <property type="entry name" value="FLGLRINGFLGH"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0975">Bacterial flagellum</keyword>
    <keyword id="KW-0998">Cell outer membrane</keyword>
    <keyword id="KW-0449">Lipoprotein</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0564">Palmitate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000236824" description="Flagellar L-ring protein">
        <location>
            <begin position="26"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="lipid moiety-binding region" description="N-palmitoyl cysteine" evidence="1">
        <location>
            <position position="26"/>
        </location>
    </feature>
    <feature type="lipid moiety-binding region" description="S-diacylglycerol cysteine" evidence="1">
        <location>
            <position position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00415"/>
        </source>
    </evidence>
    <sequence length="252" mass="27222" checksum="7F2783EF8968464C" modified="2005-10-11" version="1" precursor="true">MLKLASLNRIVLTGTLLAAAGLASGCSSIDRLSQIGESPKLTAIDNPTTQPGYKPVQMPMPKPETVSYNANSLWRNGSRAFFRDQRAARVGDLLTVTVNFTDRANIANQTQRSRVSKDDSGIADFAGSKLLGGTAQKVLPGRLLTTDSESLSDGKGLVQRQENLQTSVATVVTQVLPNGNLVVEGKQEIRVNFEIRELIVAGIVRPEDIQSDNTIDSSKIAQARIAYGGRGQITDVQQPRYGQQVMDVLLPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STV4</accession>
    <name>Q3STV4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glucokinase</fullName>
            <ecNumber evidence="1">2.7.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glucose kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">glk</name>
        <name evidence="3" type="ordered locus">Nwi_1025</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + D-glucose = ADP + D-glucose 6-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17825"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the bacterial glucokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1025"/>
    <dbReference type="EnsemblBacteria" id="ABA04287">
        <property type="protein sequence ID" value="ABA04287"/>
        <property type="gene ID" value="Nwi_1025"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1025"/>
    <dbReference type="eggNOG" id="COG0837">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042582_1_0_5"/>
    <dbReference type="OMA" id="NNHWRLS"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004340">
        <property type="term" value="F:glucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00524">
        <property type="entry name" value="Glucokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003836">
        <property type="entry name" value="Glucokinase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02685">
        <property type="entry name" value="Glucokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00749">
        <property type="entry name" value="glk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="1 3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="16"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="325" mass="34235" checksum="404D7DBE0F8BE362" modified="2005-10-11" version="1">MADPEMADSISRILLGDIGGTNARFALATDEHMGPIEKLRVTDYPDFDGALAAFLGRHRGDLPITGAVLAVAGAVEANRSVLTNSGWVIDAGRLEKSFDLSAVRVVNDFKALAWSLPYLTPPDLFAIGGGERASAAPAVVLGPGTGLGLACFVPRPRDPLAVTTEAGHVTLPGADAREDAVIAHLRERFGHVSVERALSGSGLVNLYQSLAALDHLVVPPRKPSEITEAALRGSCPTSREAVDMFCAMLGTVAGNAALTFGARGGVYIGGGIAPRIREYLACSPFRTRFEAKGRFQAYVAAIPSWVITHPDPAFVGLQRLARLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STZ1</accession>
    <name>Q3STZ1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF1244 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0988</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314289.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0988"/>
    <dbReference type="EnsemblBacteria" id="ABA04250">
        <property type="protein sequence ID" value="ABA04250"/>
        <property type="gene ID" value="Nwi_0988"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0988"/>
    <dbReference type="eggNOG" id="COG3492">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153226_0_0_5"/>
    <dbReference type="OMA" id="MPYEEWK"/>
    <dbReference type="OrthoDB" id="1902575at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023163">
        <property type="entry name" value="SMc04008-like_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036810">
        <property type="entry name" value="SMc04008-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06844">
        <property type="entry name" value="DUF1244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158757">
        <property type="entry name" value="SSF158757"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1244" evidence="2">
        <location>
            <begin position="29"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="100" mass="11315" checksum="2FFD99C9268518EF" modified="2005-10-11" version="1">MTIDDRTRTELEAAVFRRLVRHLQSRSDVQNIELMNLAGFCRNCLSNWLKEAADEQGAGMTKDESREAVYGMTYEAWKAKHQSEATPEQLAAMKKNAGGH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU08</accession>
    <name>MSRA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptide methionine sulfoxide reductase MsrA</fullName>
            <shortName evidence="1">Protein-methionine-S-oxide reductase</shortName>
            <ecNumber evidence="1">1.8.4.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Peptide-methionine (S)-S-oxide reductase</fullName>
            <shortName evidence="1">Peptide Met(O) reductase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">msrA</name>
        <name type="ordered locus">Nwi_0971</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[thioredoxin]-disulfide + H2O + L-methionyl-[protein] = [thioredoxin]-dithiol + L-methionyl-(S)-S-oxide-[protein]</text>
            <dbReference type="Rhea" id="RHEA:14217"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12315"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:44120"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[thioredoxin]-disulfide + H2O + L-methionine = [thioredoxin]-dithiol + L-methionine (S)-S-oxide</text>
            <dbReference type="Rhea" id="RHEA:19993"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58772"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MsrA Met sulfoxide reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.11" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314274.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SU08"/>
    <dbReference type="SMR" id="Q3SU08"/>
    <dbReference type="STRING" id="323098.Nwi_0971"/>
    <dbReference type="EnsemblBacteria" id="ABA04233">
        <property type="protein sequence ID" value="ABA04233"/>
        <property type="gene ID" value="Nwi_0971"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0971"/>
    <dbReference type="eggNOG" id="COG0225">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031040_10_2_5"/>
    <dbReference type="OMA" id="WVLPRRS"/>
    <dbReference type="OrthoDB" id="1554384at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033744">
        <property type="term" value="F:L-methionine:thioredoxin-disulfide S-oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008113">
        <property type="term" value="F:peptide-methionine (S)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01401">
        <property type="entry name" value="MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002569">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036509">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01625">
        <property type="entry name" value="PMSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55068">
        <property type="entry name" value="SSF55068"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00401">
        <property type="entry name" value="msrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000068346" description="Peptide methionine sulfoxide reductase MsrA">
        <location>
            <begin position="1"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01401"/>
        </source>
    </evidence>
    <sequence length="179" mass="20225" checksum="97CFE33495711B8A" modified="2005-10-11" version="1">MTVSTERAVLAGGCFWGMQALLRRYPGVISTRVGYTGGDVPDATYRNHGDHAEAIEVNFDPTVTSYRQLLEFFFQIHDPTTEDRQGYDQGLSYRSAIFYTNEEQKRIAEETIAEIDASGLWPGYVVTDVEPAGPFWEAEPEHQDYLERVPNGYTCHFVRPNWKLPRKQDAGQTAAGGDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU88</accession>
    <name>Q3SU88_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0889</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314194.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0889"/>
    <dbReference type="EnsemblBacteria" id="ABA04153">
        <property type="protein sequence ID" value="ABA04153"/>
        <property type="gene ID" value="Nwi_0889"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0889"/>
    <dbReference type="eggNOG" id="COG0446">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3453">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030742_2_2_5"/>
    <dbReference type="OMA" id="FPWVYYN"/>
    <dbReference type="OrthoDB" id="403436at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005939">
        <property type="entry name" value="BLH_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015904">
        <property type="entry name" value="Sulphide_quinone_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10632">
        <property type="entry name" value="PTHR10632"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04273">
        <property type="entry name" value="DUF442"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01244">
        <property type="entry name" value="TIGR01244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF442" evidence="1">
        <location>
            <begin position="4"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Pyr_redox_2" evidence="2">
        <location>
            <begin position="150"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="554" mass="59734" checksum="BB857DB40644E8FB" modified="2005-10-11" version="1">MDTKKLSDELSVSAQVMPQDMAALKQSGFRSIICNRPDGEAADQPSARELELAASSHGLEFVHQPVVAGKMSDDDAVQFAQTLKSLPKPTLAFCRTGTRSISLWCLANASTQPLPALLGAAKAAGYDMSGIVRRIANGGRTPTRLPDASYDVVIVGGGAAGIACASSLLARAPDLDIAVIDPADIHYYQPGWTMVGAGIFDATSTVHTMASVVPSGVTWIKAAVTAFEPAENAVILEGCRVVKYSRLVVCPGLKLDWEKVDGLTETLGRNGVTSNYRFDLAPYTWKLVRSLKSGNAVFTQPPMPIKCAGAPQKAMYLSADHWHRQGHLRGIDVGFYNAGGVLFGVADYVPALMEYVRKYDAKLKFQHDLVAIDGPDKKAYFTKTSADGPAETVEVQFDMIHVTPPQCAPDFIRVSPLANAAGWVDVDDGTLRHKAYANIWSLGDVCSAPNAKTAAAARKQAPIVAENICVDLGLRAKQAVYDGYGACPLTVERGRIVLAEFGYGGKLLPSFPTFILDGRKPTRAAWWLKERVLPPIYWHAMLKGKEWMAAPDVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUB3</accession>
    <name>Q3SUB3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Beta-lactamase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0864</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314173.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0864"/>
    <dbReference type="EnsemblBacteria" id="ABA04128">
        <property type="protein sequence ID" value="ABA04128"/>
        <property type="gene ID" value="Nwi_0864"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0864"/>
    <dbReference type="eggNOG" id="COG2333">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065110_0_0_5"/>
    <dbReference type="OMA" id="TSRENEM"/>
    <dbReference type="OrthoDB" id="1796527at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="289" mass="32168" checksum="415D2E37E3C37B98" modified="2005-10-11" version="1">MAFSVEFLPVGDSDGDAILIQYGTENSWYLNIVDGGYASIGEKVVEHIHEHYGRDININNMVVSHADNDHAAGLIEVFKNFRVGTLWMNRPWLYANEVIDQFHGNWTVEGWIKHVRGTHDYLVELEDLARARGMEPQEVFQGAQIGPCYVLAPSRSRYISLIPNLDKTPPPYKSDATPRSIFETARSLLDRIKETLQIETLDANPPATSASNETSVVQLAVYESHKVLLTADAGPAALTEAANYAHLLGMLSAPNLVQIPHQGSRRNVTPAVLNAWCPSSDNLRLIRHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUD8</accession>
    <name>Q3SUD8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0839</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04103">
        <property type="protein sequence ID" value="ABA04103"/>
        <property type="gene ID" value="Nwi_0839"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0839"/>
    <dbReference type="HOGENOM" id="CLU_2494759_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="86" mass="9135" checksum="A92D7959EC77D48B" modified="2005-10-11" version="1">MAVRRRDQMSAMSSPSLSTGLASSRPERGNSDRPACKGKAAIALLTLDAFSHAAPNAANHHGEQTGGNQNRSHSCLFTAGKELHRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUF2</accession>
    <name>Q3SUF2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH_17 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0824</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0824"/>
    <dbReference type="EnsemblBacteria" id="ABA04089">
        <property type="protein sequence ID" value="ABA04089"/>
        <property type="gene ID" value="Nwi_0824"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0824"/>
    <dbReference type="eggNOG" id="ENOG50337AK">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_180003_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041657">
        <property type="entry name" value="HTH_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12728">
        <property type="entry name" value="HTH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_17" evidence="2">
        <location>
            <begin position="37"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="101" mass="11385" checksum="3D411B70E942EC15" modified="2005-10-11" version="1">MVPLRSSEPQGHKETGMDDQVNAQRNGAASAELMQGWMGRREVAEALGISTATLQRWQTRRIGPPCVRIGRRVFYRADAFREWMISQERGPVVSKRTGSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUF4</accession>
    <name>Q3SUF4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0822</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314135.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0822"/>
    <dbReference type="EnsemblBacteria" id="ABA04087">
        <property type="protein sequence ID" value="ABA04087"/>
        <property type="gene ID" value="Nwi_0822"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0822"/>
    <dbReference type="eggNOG" id="ENOG5033V2M">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1064893_0_0_5"/>
    <dbReference type="OrthoDB" id="1969880at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="261" mass="28317" checksum="50085AA1979E4FAD" modified="2005-10-11" version="1">MSNPFERHDIGHLSASSLNLWAAEPALWIMERLLGRRSPSGIPAARGKAVEAGVNIGLHDPALPVEACIAEAESAFDRETALNPDPRRDDERKKLPGYVRGALAELRQYGLPDADGYQGKVEIRLDDIAVPVIGFVDWRFSGHGLIVDLKTTERLPSVIGASHGRQGAVYATAHGNFGMRFAYAKPAPAKGDGRQVQVLEMSGDDVRAHLAALRLIALSLGRFLALSHDARELAGLIVPDFDSFYWSDPSVRAAGRDVFGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUK0</accession>
    <name>Q3SUK0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Respiratory nitrate reductase beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0776</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314090.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0776"/>
    <dbReference type="EnsemblBacteria" id="ABA04041">
        <property type="protein sequence ID" value="ABA04041"/>
        <property type="gene ID" value="Nwi_0776"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0776"/>
    <dbReference type="eggNOG" id="COG1140">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043374_5_2_5"/>
    <dbReference type="OMA" id="VYFNWQT"/>
    <dbReference type="OrthoDB" id="1762646at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009325">
        <property type="term" value="C:nitrate reductase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008940">
        <property type="term" value="F:nitrate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042126">
        <property type="term" value="P:nitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10557">
        <property type="entry name" value="NarH_beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029263">
        <property type="entry name" value="Nitr_red_bet_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006547">
        <property type="entry name" value="NO3_Rdtase_bsu"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13247">
        <property type="entry name" value="Fer4_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14711">
        <property type="entry name" value="Nitr_red_bet_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01660">
        <property type="entry name" value="narH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="7"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="179"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="513" mass="58031" checksum="E71359949F0B6634" modified="2005-10-11" version="1">MDIRAQVSMVFHLDKCIGCHTCSIACKNIWTDRKGTEYMYWNNVETKPGTGYPTRWEDQTKYRGGWVVDGQRQKSLRLRLQGKWGTLTNIFYNPYLPTLDDYFEPWTYDYQNLITAPLADEQPTARAISMVTGKYMDTIEAGPNWDDDLGGSQVYANNDPNFDGASDEEMRQINEINSTVFFYLPRICNHCLNPGCVAACPQGALYKRGEDGVVLVSQERCRAWRMCVSGCPYKKTYFNWSTGKAEKCILCYPRLESGQPPACFHSCVGRIRYIGLVLYDADAIEETAKSPQDQLVMAQRNIIKDPFDPEIIAAARANGIPDSKIEAAQKSPVFQFVKKWGIALPLHPEFRTLPMLFYVPPLGPVLAKVENGVYDNVANEARLGPLMSSLERSRIPLRYMASLLAGGNEEIIRDVYKKLVAVRVYMRSRKVKDIPDEEVQRALSEGKTTAAEVEAIWRLTSMPTFEERFVVPPMERETAVDALFPQLDPVSHNYPIRKGEVGVGFHTDPARGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUK4</accession>
    <name>Q3SUK4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0772</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314086.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04037">
        <property type="protein sequence ID" value="ABA04037"/>
        <property type="gene ID" value="Nwi_0772"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0772"/>
    <dbReference type="HOGENOM" id="CLU_1119251_0_0_5"/>
    <dbReference type="OMA" id="SIRICPV"/>
    <dbReference type="OrthoDB" id="2108605at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="248" mass="27851" checksum="179B378C7D6851E3" modified="2005-10-11" version="1">MAKSRLPAFLLDHPGLGSLGNVEVPRLKLLQASSPELAQFENAREGEFWHSLLGRSIGASIRICPVFINWCFILWRPREAGGGILARADDSKHWIPADTEFTVKLKSGDEVTWRTGRTVTASGLNKRGSYNPNDPNSPPAATRLYSVVCSFPDHQNWPPAVITLQRRSEKAASKLIDQLKVLRAPSFGVILELSSFKDESPSGEFNNYAFELYGPVEDKPFYEENSRQYRFFIEHGLKVKDLESAQED</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="126" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUL4</accession>
    <name>Q3SUL4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome c oxidase subunit 1</fullName>
            <ecNumber evidence="9">7.1.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_0224</name>
        <name evidence="12" type="ordered locus">Nwi_0762</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="12">Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 9">
            <text>4 Fe(II)-[cytochrome c] + 8 H(+)(in) + O2 = 4 Fe(III)-[cytochrome c] + 4 H(+)(out) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11436"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="7.1.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cell membrane</location>
            <topology evidence="2 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the heme-copper respiratory oxidase family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.9" evidence="9"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313559.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0224"/>
    <dbReference type="EnsemblBacteria" id="ABA03492">
        <property type="protein sequence ID" value="ABA03492"/>
        <property type="gene ID" value="Nwi_0224"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04027">
        <property type="protein sequence ID" value="ABA04027"/>
        <property type="gene ID" value="Nwi_0762"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0224"/>
    <dbReference type="KEGG" id="nwi:Nwi_0762"/>
    <dbReference type="eggNOG" id="COG0843">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011899_7_3_5"/>
    <dbReference type="OMA" id="PVDFQYH"/>
    <dbReference type="OrthoDB" id="316745at2"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006119">
        <property type="term" value="P:oxidative phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01663">
        <property type="entry name" value="Cyt_c_Oxidase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.210.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023616">
        <property type="entry name" value="Cyt_c_oxase-like_su1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036927">
        <property type="entry name" value="Cyt_c_oxase-like_su1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000883">
        <property type="entry name" value="Cyt_C_Oxase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023615">
        <property type="entry name" value="Cyt_c_Oxase_su1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033944">
        <property type="entry name" value="Cyt_c_oxase_su1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014241">
        <property type="entry name" value="Cyt_c_oxidase_su1_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422">
        <property type="entry name" value="PTHR10422"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00115">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01165">
        <property type="entry name" value="CYCOXIDASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81442">
        <property type="entry name" value="SSF81442"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02891">
        <property type="entry name" value="CtaD_CoxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50855">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00077">
        <property type="entry name" value="COX1_CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0186">Copper</keyword>
    <keyword evidence="8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0349">Heme</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="85"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="171"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="209"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="256"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="326"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="363"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="396"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="437"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="478"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="COX1" evidence="10">
        <location>
            <begin position="24"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA03492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA04027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="539" mass="59161" checksum="D3631A3DBE1832FC" modified="2005-10-11" version="1">MATASAHDHAHDDHGDHAHHPTGWKRFAYSTNHKDIGTMYLVFAIVAGIIGGLMSMGIRAELMYPGIQVFDPAHTYNVFATNHGLIMIFFMVMPAMIGGFGNWFVPLMIGAPDMAFPRMNNISFWLLPAAFALLLISSFMEGEPGATGAGTGWTLYAPLSTSGHPGPAVDFSILSIHLAGASSILGAINFITTIFNMRAPGMTMHKMPLFVWSILVTVFLLLLSLPVLAGAITMLLTDRNFGTTFFAADGGGDPVLFQHLFWFFGHPEVYILILPGFGMISHVISTFSKKPIFGYLGMAYAMVAIGGIGFVVWAHHMYTVGMVSSAQAYFVAATMVIAVPTGVKVFSWIATMWGGSIEFKTPMLFAIGFIFLFTVGGVTGVVLANAGVDRVMQETYYVIAHFHYVMGIAAVFAIFSGWYYWFPKMSGYMYNETIGKLHFWVMFIGANILFFPQHFLGLQGMMRRMVDYPDAFAGWNEISSYGAFITGFGVIIFLYGLVDAFARKQQAADNPWGEGATTLEWTLSSPPPFHQFSTLPKVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUR1</accession>
    <name>Q3SUR1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_0714</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314034.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0714"/>
    <dbReference type="EnsemblBacteria" id="ABA03980">
        <property type="protein sequence ID" value="ABA03980"/>
        <property type="gene ID" value="Nwi_0714"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0714"/>
    <dbReference type="eggNOG" id="COG5567">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155853_0_0_5"/>
    <dbReference type="OMA" id="GCGRKAG"/>
    <dbReference type="OrthoDB" id="1960469at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032831">
        <property type="entry name" value="LPAM_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13627">
        <property type="entry name" value="LPAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0564">Palmitate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="41"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA03980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="101" mass="10354" checksum="C4D16B70CA12DD59" modified="2005-10-11" version="1">MVPPIGDIAVNSTSSSISMRWALFLLTAAALALAGCGRKGGLDVPPTASNQPPAAAAADDHDRANAPKDNLFDPSFGINSDPAASRGARKSFILDPLLDSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUR3</accession>
    <name>Q3SUR3_NITWN</name>
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        <submittedName>
            <fullName evidence="8">Cytochrome c, class I</fullName>
        </submittedName>
    </protein>
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        <name evidence="8" type="ordered locus">Nwi_0712</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0712"/>
    <dbReference type="EnsemblBacteria" id="ABA03978">
        <property type="protein sequence ID" value="ABA03978"/>
        <property type="gene ID" value="Nwi_0712"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0712"/>
    <dbReference type="eggNOG" id="COG3474">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060944_4_0_5"/>
    <dbReference type="OMA" id="VDFATIM"/>
    <dbReference type="OrthoDB" id="1939800at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002327">
        <property type="entry name" value="Cyt_c_1A/1B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11961">
        <property type="entry name" value="PTHR11961"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00034">
        <property type="entry name" value="Cytochrom_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00604">
        <property type="entry name" value="CYTCHRMECIAB"/>
    </dbReference>
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        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cytochrome c" evidence="7">
        <location>
            <begin position="75"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA03978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="184" mass="19212" checksum="8A969C7D595D5905" modified="2005-10-11" version="1">MSSGSDTVHSNRTNAIMGALLGTLLFVQTVRIIDESFSAPEKPHAEASHKTEAKEAAAAPSAADAESFNKALASASAERGAGLVKPCLACHTTEEGGAKKVGPNLYGIVNRPIASEQGFNYSAALKSKAGGKWTFADLSSWLDDPKAYAKGTSMGYPGLKSESKRADLIAYLNTLSKDPAPLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUS3</accession>
    <name>Q3SUS3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">FMN-dependent alpha-hydroxy acid dehydrogenase</fullName>
            <ecNumber evidence="7">1.1.3.15</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0702</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.3.15" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314024.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0702"/>
    <dbReference type="EnsemblBacteria" id="ABA03968">
        <property type="protein sequence ID" value="ABA03968"/>
        <property type="gene ID" value="Nwi_0702"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0702"/>
    <dbReference type="eggNOG" id="COG1304">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020639_0_0_5"/>
    <dbReference type="OMA" id="AWSWEKI"/>
    <dbReference type="OrthoDB" id="1186741at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003973">
        <property type="term" value="F:(S)-2-hydroxy-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02809">
        <property type="entry name" value="alpha_hydroxyacid_oxid_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012133">
        <property type="entry name" value="Alpha-hydoxy_acid_DH_FMN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000138">
        <property type="entry name" value="Al-hdrx_acd_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0288">FMN</keyword>
    <keyword evidence="2 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="6">
        <location>
            <begin position="10"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="262"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="89"/>
            <end position="91"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="293"/>
            <end position="297"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="316"/>
            <end position="317"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="369" mass="39159" checksum="B81334B598EAF8BC" modified="2005-10-11" version="1">MTTPLAKLTQIPPTVAAVADYEVLARERVSPGAWAYLDGGAADEVTARENRAAFARLHLRTRVLRDLSSGNTACELFGTRLRAPILLAPVAYQKLAYPDGELATVLGASAMRMAMVVSTQASVALEEIAREAQTPLWFQLYIQHDRDFTLRLVRRAESAGIRALVVSVDAPISGLRNREQRMGFAFPGGIEPVNLRGLTPSPRAAGETLFDSPLITRAATWRDIENLREATKLPLVLKGIMTAEDAEQALAAGVDGLIVSNHGGRVLDGQPATIEVLPEIAAAVSGRVPILLDGGIRRGGDVFKALALGASAVLVGRAFVHGLAAAGAVGVAHVLRILHAELEATMVLTGCRDIRAISPASIRQAGLRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUW3</accession>
    <name>Q3SUW3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, XRE family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0662</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0662"/>
    <dbReference type="EnsemblBacteria" id="ABA03928">
        <property type="protein sequence ID" value="ABA03928"/>
        <property type="gene ID" value="Nwi_0662"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0662"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1917">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085376_5_0_5"/>
    <dbReference type="OMA" id="GMLIQIE"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="82"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="259" mass="28413" checksum="233D88D129E9C25C" modified="2005-10-11" version="1">MRSEIRRSLWSVIGQPIPIRFSVHVADGACRHCVPDMGIKARIHGVRPDHRMSKLGTSQIYHQGDGSGLDCIELPVIVGKNLRRIRLRQGLSLERLAKQSGVSRAMLGQIETGKSTPTIGLLWKICTSLTVPFASLIAVGDPEGIIVLRRDGSKLLSSNQGRFTSRALFPFDSERRTEFYELRLAPLYCENAEAHAAGTRENLVVAKGSVEIAVGSARPTTLSEGDAMLFEADVPHAYRNLGTDEAVLYLVMTYAQMIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV06</accession>
    <name>Q3SV06_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Extensin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0618</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0618"/>
    <dbReference type="EnsemblBacteria" id="ABA03885">
        <property type="protein sequence ID" value="ABA03885"/>
        <property type="gene ID" value="Nwi_0618"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0618"/>
    <dbReference type="eggNOG" id="COG3921">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_949392_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009683">
        <property type="entry name" value="Extensin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06904">
        <property type="entry name" value="Extensin-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Extensin-like_C" evidence="2">
        <location>
            <begin position="21"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="293" mass="32251" checksum="F4C530EA6D0066D8" modified="2005-10-11" version="1">MRWPSLRAFRLSQGLARAGGTDLVRLEAVVLPDRTRVPVKPAATLRCTMASAIADWVRADIAPLAERLGSRVSELDNFDSFECRGRNRVAGAKLSEHGHANALDVRSVRMSNGRTITLTDRSVPRELREKLLNSVCARFTTVLGPGSDGYHEDHIHLDLAGRRNGYRICQWKIDDPMPRVAPLLPPERPAKTSLREPAEEKNKKEKASEGVPERAAASGVASQASDDPPQLPAAVPIPQKRPLSRTEPGKVAEDAKPSPSVSRSSTRLSATRSAKRKHRPRLDLPPFLRPLFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV24</accession>
    <name>Q3SV24_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0600</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_002714638.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0600"/>
    <dbReference type="EnsemblBacteria" id="ABA03867">
        <property type="protein sequence ID" value="ABA03867"/>
        <property type="gene ID" value="Nwi_0600"/>
    </dbReference>
    <dbReference type="GeneID" id="66477638"/>
    <dbReference type="KEGG" id="nwi:Nwi_0600"/>
    <dbReference type="eggNOG" id="ENOG5031CDG">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161849_0_0_5"/>
    <dbReference type="OMA" id="DEACQRY"/>
    <dbReference type="OrthoDB" id="1937508at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009534">
        <property type="entry name" value="DUF1153"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010921">
        <property type="entry name" value="Trp_repressor/repl_initiator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06627">
        <property type="entry name" value="DUF1153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48295">
        <property type="entry name" value="SSF48295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="91" mass="10277" checksum="A90790ADD94A990C" modified="2005-10-11" version="1">MTEPHRPRVKYVIGPDGSPLTIADLPAPGTKRWVIRRKAEVVAAVRGGLLSLEEACSRYTLTVDEFLSWQFSIDQHGLAGLRTTRIQQYRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV67</accession>
    <name>Q3SV67_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NnrS</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0557</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313885.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0557"/>
    <dbReference type="EnsemblBacteria" id="ABA03824">
        <property type="protein sequence ID" value="ABA03824"/>
        <property type="gene ID" value="Nwi_0557"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0557"/>
    <dbReference type="eggNOG" id="COG3213">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041785_2_0_5"/>
    <dbReference type="OMA" id="HVHEMLY"/>
    <dbReference type="OrthoDB" id="791105at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010266">
        <property type="entry name" value="NnrS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05940">
        <property type="entry name" value="NnrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="244"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="272"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="302"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="338"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="363"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="402" mass="43111" checksum="5F17F49F4F011DE5" modified="2005-10-11" version="1">MAMSDVENAKAEPLAIWTIGFRPFFLMAGIWSAVALAGWIAVLTIGLTLPSRFDPLTWHIHEMLFGFVLAAIAGFLLTAIPNWTGRPPISGRLLAALVMLWFAGRVTCLVSAWFPLGLAAAIDLAFPFVLCGVVAREIIAGRNWRNMIMPIPVAMLGVADLLMYLELSGSSLSVGLGWRLGLAAIIVLISVVGGRIIPSFTRNWLIRQNASRLPPAHGHVDSLALVSLLAGMIGWALFPERWPFGWLLLVAAALNLWRLGRWRGPAVGAEPLLTILHAGYLWVIIGAALLGASMLADHVPQAAAIHALTAGAIGTMTLGVMTRVSLGHTGRALTADRITGLIYLLVILAAIARVAAWDGGSSAMLIEISAVLWIGSFGLFVLRYGPILLSPRVKSVDIQPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV95</accession>
    <name>Q3SV95_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Flagellar biosynthesis protein FlhA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">flhA</name>
        <name evidence="8" type="ordered locus">Nwi_0529</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for formation of the rod structure of the flagellar apparatus. Together with FliI and FliH, may constitute the export apparatus of flagellin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the FHIPEP (flagella/HR/invasion proteins export pore) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313857.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0529"/>
    <dbReference type="EnsemblBacteria" id="ABA03796">
        <property type="protein sequence ID" value="ABA03796"/>
        <property type="gene ID" value="Nwi_0529"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0529"/>
    <dbReference type="eggNOG" id="COG1298">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015346_3_0_5"/>
    <dbReference type="OMA" id="MAINPGQ"/>
    <dbReference type="OrthoDB" id="436367at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044780">
        <property type="term" value="P:bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042194">
        <property type="entry name" value="FHIPEP_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042193">
        <property type="entry name" value="FHIPEP_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042196">
        <property type="entry name" value="FHIPEP_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006301">
        <property type="entry name" value="FlhA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001712">
        <property type="entry name" value="T3SS_FHIPEP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30161">
        <property type="entry name" value="PTHR30161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00771">
        <property type="entry name" value="FHIPEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005419">
        <property type="entry name" value="FlhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00949">
        <property type="entry name" value="TYPE3IMAPROT"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01398">
        <property type="entry name" value="FlhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword evidence="7" id="KW-1006">Bacterial flagellum protein export</keyword>
    <keyword evidence="3 7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="8" id="KW-0282">Flagellum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="127"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="220"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="261"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA03796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="709" mass="74985" checksum="D1741B607C0DA28A" modified="2005-10-11" version="1">MKGVTADQGATVPASGFPTFSQIGIMLKRGDLALAFGILTILVVLIMPLPAMVLDLLLAISITLSILILMTALFIQSPLEFSSFPTVLLIATMLRLSLNLASTRLILSDGHEGSAAAGHVIEAFGNFVMGGNFVIGIIVFGILVIVNFVVITKGSGRIAEVAARFQLDSMPGKQMAIDADLSAGLIDEKTAKERRKAIEDESGFFGAMDGASKFVRGDAVAGLLVVFINIIGGMIIGIAQQDLSFADAAHSYTILTVGDGLVTQIPALIVSTAAGLLVSKAAVTGAADKALMKQLSGYPQALGMSAGVMLVLGLLPGIPMLPFVALGGGAAALAWSSRKRQSAAAAEAVAAAASETACIAAANVEEPISAALRIDDLKIELGYALLPLVNGPDGQDRLTEQIKALRRSLAIEMGFVMPAVRILDNVQLEANTYIIKIKEVDAGSGRIWPNQYMVMDPAGGQVGVPGIHTTEPTFGLPATWVDASLKEEASLKGYTVVDSATVVSTHLTELLKVNMSDLLSYGEVQKLLKDLPKEQGELIKDIVPGQISISGIQRVLQLLLAERISVRDLSTILEGIAEALAFSRNPATLVEHVRARLARQICAQNTSVNGYLPLVALSAKWEQAFAESIIGQGEERSLAMQPSKLSEFMAAVRDRFEQAAREGEAPVLVTSAAIRPFVRSLIERFRAQTPVLSQAEIHPRARLKTVGSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVL9</accession>
    <name>Q3SVL9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phytase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0405</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313736.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0405"/>
    <dbReference type="EnsemblBacteria" id="ABA03672">
        <property type="protein sequence ID" value="ABA03672"/>
        <property type="gene ID" value="Nwi_0405"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0405"/>
    <dbReference type="eggNOG" id="COG4246">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059147_0_0_5"/>
    <dbReference type="OMA" id="SDDNFSM"/>
    <dbReference type="OrthoDB" id="1884225at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027372">
        <property type="entry name" value="Phytase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014567">
        <property type="entry name" value="UCP031900"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13449">
        <property type="entry name" value="Phytase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031900">
        <property type="entry name" value="UCP031900"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229183" description="Phytase-like domain-containing protein" evidence="1">
        <location>
            <begin position="29"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Phytase-like" evidence="2">
        <location>
            <begin position="88"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="356" mass="38759" checksum="EE30DBE9D9D13ECC" modified="2005-10-11" version="1">MASWIDRRRLLFLTASSLLLTGSSVAPAQFATHAPEQPPPDELTPPAPVSIAVNARPVAAFDLRDRSRTRFGSLAFRGGLILTSAFRGFGGLSSLRLDSKGRQFISASDKGAWFTGRIIYAASGEMVGLADVEAAPMLGRDGRLITERGWFDTESIARDGSLIYVGIERANRIMRFDFSKGYTRARGEEVEAPAAIRRLPFNRGLEAMVVVPRDRPLGGTLIAISERGLDAERNILAFLIGGPSPGRFTIRRTENYDISDACLLPSGDLLILERKFSLTGGIGIRIRRVRIDAIAPGALVDGPSIFDAGLGQEIDNYEGLSAFVNDDGEIVLTLVSDDNFSLFQRTLLMQFTLAEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVP0</accession>
    <name>Q3SVP0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase</fullName>
            <ecNumber evidence="4">3.5.1.77</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0384</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the carbon-nitrogen hydrolase superfamily. NIT1/NIT2 family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.77" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0384"/>
    <dbReference type="EnsemblBacteria" id="ABA03651">
        <property type="protein sequence ID" value="ABA03651"/>
        <property type="gene ID" value="Nwi_0384"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0384"/>
    <dbReference type="eggNOG" id="COG0388">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030130_1_2_5"/>
    <dbReference type="OMA" id="GNTYRES"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047417">
        <property type="term" value="F:N-carbamoyl-D-amino acid hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07572">
        <property type="entry name" value="nit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045254">
        <property type="entry name" value="Nit1/2_C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001110">
        <property type="entry name" value="UPF0012_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="SSF56317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01227">
        <property type="entry name" value="UPF0012"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CN hydrolase" evidence="3">
        <location>
            <begin position="14"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="300" mass="32771" checksum="06B5610AF9252406" modified="2005-10-11" version="1">MRAEGKDMNTQPTFVAAMIQMRSGLLPEPNFEQGSRLIREAAAQGADFVQTPEVSNIMQADRAALFEHLRAEDDDRSLSGYRDLARELKIHLNVGSLALRLTKEKAVNRSFLIGPSGDILARYDKIHMFDIDLDGGESYRESANYQPGETAAIADLPWGRLGMTICYDVRFPALYRALAEAGASFLTVPAAFTRKTGEAHWHTLLRARAIENGCFVFAAAQAGMHENNRETFGHSLIVDPWGTVLADAGGAEPGIVLATIDPGKVLAVRKAVPSLQHGRRFSLADHKPASDHLHLVRGSA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVP3</accession>
    <name>UBIG_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquinone biosynthesis O-methyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">2-polyprenyl-6-hydroxyphenol methylase</fullName>
            <ecNumber evidence="1">2.1.1.222</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">3-demethylubiquinone 3-O-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.64</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ubiG</name>
        <name type="ordered locus">Nwi_0381</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">O-methyltransferase that catalyzes the 2 O-methylation steps in the ubiquinone biosynthetic pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 3-demethylubiquinol + S-adenosyl-L-methionine = a ubiquinol + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:44380"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="Rhea" id="RHEA-COMP:10914"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:84422"/>
            <dbReference type="EC" id="2.1.1.64"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 3-(all-trans-polyprenyl)benzene-1,2-diol + S-adenosyl-L-methionine = a 2-methoxy-6-(all-trans-polyprenyl)phenol + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:31411"/>
            <dbReference type="Rhea" id="RHEA-COMP:9550"/>
            <dbReference type="Rhea" id="RHEA-COMP:9551"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:62729"/>
            <dbReference type="ChEBI" id="CHEBI:62731"/>
            <dbReference type="EC" id="2.1.1.222"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. UbiG/COQ3 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.222" evidence="1"/>
    <dbReference type="EC" id="2.1.1.64" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313712.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVP3"/>
    <dbReference type="SMR" id="Q3SVP3"/>
    <dbReference type="STRING" id="323098.Nwi_0381"/>
    <dbReference type="EnsemblBacteria" id="ABA03648">
        <property type="protein sequence ID" value="ABA03648"/>
        <property type="gene ID" value="Nwi_0381"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0381"/>
    <dbReference type="eggNOG" id="COG2227">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042432_0_0_5"/>
    <dbReference type="OMA" id="RGTHDWE"/>
    <dbReference type="OrthoDB" id="1515497at2"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102208">
        <property type="term" value="F:2-polyprenyl-6-hydroxyphenol methylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008425">
        <property type="term" value="F:2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008689">
        <property type="term" value="F:3-demethylubiquinone-9 3-O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00472">
        <property type="entry name" value="UbiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010233">
        <property type="entry name" value="UbiG_MeTrfase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01983">
        <property type="entry name" value="UbiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="chain" id="PRO_0000241713" description="Ubiquinone biosynthesis O-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="261" mass="28745" checksum="741EC000B02517D4" modified="2005-10-11" version="1">MTMQVDPSANSSAASSAAPGTTVDRAEIEKFSKLSQEWWDPTGKMAPLHRINPLRLQFIRDAACRKFDRNARSLNCLAGLRLLDIGCGAGLLCEPFTRLGAQVIGVDPSASNIAAAKLHAEKAHLSIDYRCTTVEDMDVRERFDIILAMEVVEHVADVGLFLDRCAAMLKPGGMMAASTLNRNWKSFALGIVAAEYVLRWLPRGTHQWDKFVTPDELARHFERNGLGITEQSGVVYSPFGDRWSLSSDMDVNYMVVAEAVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVQ4</accession>
    <name>Q3SVQ4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Histidine triad (HIT) protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0370</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313701.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03637">
        <property type="protein sequence ID" value="ABA03637"/>
        <property type="gene ID" value="Nwi_0370"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0370"/>
    <dbReference type="eggNOG" id="COG0537">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123330_0_1_5"/>
    <dbReference type="OMA" id="NYPWFIL"/>
    <dbReference type="OrthoDB" id="1598286at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026026">
        <property type="entry name" value="HIT_Hint"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000714">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="SSF54197"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="2">
        <location>
            <begin position="37"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="138" mass="15096" checksum="81A557B71592CBBC" modified="2005-10-11" version="1">MSETWSLHPQLDNDTVGIGDLSLSRLALSKDATYPWLILAPRRAGAIEIIDLDHDDRSRLMTEIARASEVLKGITGCDKLNVAALGNQVPQLHVHVIARRKSDAAWPNPVWGAAPAREYDAAELEKIIEAIRRGIGLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVW0</accession>
    <name>Q3SVW0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">DEAD/DEAH box helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_0314</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313646.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0314"/>
    <dbReference type="EnsemblBacteria" id="ABA03581">
        <property type="protein sequence ID" value="ABA03581"/>
        <property type="gene ID" value="Nwi_0314"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0314"/>
    <dbReference type="eggNOG" id="COG0513">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_28_3_5"/>
    <dbReference type="OMA" id="EHKDGQR"/>
    <dbReference type="OrthoDB" id="643629at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6 11" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Q_MOTIF" evidence="10">
        <location>
            <begin position="8"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="39"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="241"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="378"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="5">
        <location>
            <begin position="8"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA03581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="476" mass="51756" checksum="49F49E507E7F6DBF" modified="2005-10-11" version="1">MERSNLLSSFQDFGLADPISRALAEENYLTPTPIQAQTIPIALTGRDLVGIAQTGTGKTAAFALPILHRLLQNRIKPQPKSCRVLVLSPTRELSGQILENFNAYGRHLRLTSALAIGGVPMGRQVRAVMHGVEVMVATPGRLLDLVQSNGLKLGQVEFLVLDEADRMLDMGFIHDIRKVVAKLPAKRQTLFFSATMPKDIAELANQMLRDPARVAVTPVSSTVDRITQRIIQVDHAGKAAFLAQLLKQEPVNRALIFTRTKHGADKVVKSLAKSGIRSNAIHGNKSQNHRERVLAEFRAGEIRTLVATDIAARGIDVDGISHVVNFDLPNVPETYVHRIGRTARAGTDGAAISLVAGAEEMAYLRDIEKLIRVVLPREDRRTSGQREAAPAPQQNRGGSPAPHGHAARAGDASSGKGSRRRRSKKGTPQTSRRPESGRQETSRSAPGSKPGGMQGVAFLHRKSRPARLKSSQPPQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW29</accession>
    <name>Q3SW29_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Possible outer membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0244</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0244"/>
    <dbReference type="TCDB" id="1.B.4.2.8">
        <property type="family name" value="the brucella-rhizobium porin (brp) family"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03512">
        <property type="protein sequence ID" value="ABA03512"/>
        <property type="gene ID" value="Nwi_0244"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0244"/>
    <dbReference type="eggNOG" id="COG3637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037100_0_1_5"/>
    <dbReference type="OMA" id="WSGKVEY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027385">
        <property type="entry name" value="OMP_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13505">
        <property type="entry name" value="OMP_b-brl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229191" evidence="1">
        <location>
            <begin position="40"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="OMP_b-brl" evidence="2">
        <location>
            <begin position="39"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="241" mass="25749" checksum="439ACA5E8D410D11" modified="2005-10-11" version="1">MTGPTNGCSELEFLENEMKKILLASVAFLGMAAIVPAQAADLAPRPYTKAPPYVPAPIYNWTGFYLGGHIGGAFGGPGNVLAPGFFNNNDGAFMGGGQVGYDTQFSPNWVFGIEANYSFLDTGSFFANRGLGSVTARLGYTWGPALLYVKGGYGWADSRFTNGFAGNGGRDGYTVGGGLEYLFTQNWSGKVEYQYYDLGNVNFFGPLGGIGNFRNDQHTVKVGLNYRFNFGAPAAPYAPGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW36</accession>
    <name>Q3SW36_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP synthase subunit c</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">ATP synthase F(0) sector subunit c</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">F-type ATPase subunit c</fullName>
            <shortName evidence="10">F-ATPase subunit c</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Lipid-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">atpE</name>
        <name evidence="12" type="ordered locus">Nwi_0237</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Key component of the F(0) channel; it plays a direct role in translocation across the membrane. A homomeric c-ring of between 10-14 subunits forms the central stalk rotor element with the F(1) delta and epsilon subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">F-type ATPases have 2 components, F(1) - the catalytic core - and F(0) - the membrane proton channel. F(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). F(0) has three main subunits: a(1), b(2) and c(10-14). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. F(1) is attached to F(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell inner membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ATPase C chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009796340.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0237"/>
    <dbReference type="EnsemblBacteria" id="ABA03505">
        <property type="protein sequence ID" value="ABA03505"/>
        <property type="gene ID" value="Nwi_0237"/>
    </dbReference>
    <dbReference type="GeneID" id="66891862"/>
    <dbReference type="KEGG" id="nwi:Nwi_0237"/>
    <dbReference type="eggNOG" id="COG0636">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148047_4_0_5"/>
    <dbReference type="OMA" id="CMGFCIL"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045263">
        <property type="term" value="C:proton-transporting ATP synthase complex, coupling factor F(o)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01396">
        <property type="entry name" value="ATP_synth_c_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000454">
        <property type="entry name" value="ATP_synth_F0_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038662">
        <property type="entry name" value="ATP_synth_F0_csu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10031">
        <property type="entry name" value="PTHR10031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00124">
        <property type="entry name" value="ATPASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="SSF81333"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="10" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 10" id="KW-0138">CF(0)</keyword>
    <keyword evidence="5 10" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="50"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-synt_C" evidence="11">
        <location>
            <begin position="9"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="site" description="Reversibly protonated during proton transport" evidence="10">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA03505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="75" mass="7445" checksum="8425C3663626F1C5" modified="2005-10-11" version="1">MDPIAAKYIGAGIACIGMGGAGAGVGIIFGNYLAAAVRNPSAAQGQFGNLIFGFAVTEALGIFSLLIALLLLFAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW74</accession>
    <name>Q3SW74_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ribosomal RNA adenine methylase transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0199</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313535.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0199"/>
    <dbReference type="EnsemblBacteria" id="ABA03467">
        <property type="protein sequence ID" value="ABA03467"/>
        <property type="gene ID" value="Nwi_0199"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0199"/>
    <dbReference type="eggNOG" id="COG3963">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085338_0_0_5"/>
    <dbReference type="OMA" id="RIWMNLP"/>
    <dbReference type="OrthoDB" id="2030110at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000179">
        <property type="term" value="F:rRNA (adenine-N6,N6-)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020596">
        <property type="entry name" value="rRNA_Ade_Mease_Trfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020598">
        <property type="entry name" value="rRNA_Ade_methylase_Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="PTHR11727"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00650">
        <property type="entry name" value="rADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01131">
        <property type="entry name" value="RRNA_A_DIMETH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="rADc" evidence="4">
        <location>
            <begin position="43"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="200" mass="22082" checksum="10EFC24C9F1F2F99" modified="2005-10-11" version="1">MPLQLSARASKKPLHLDDEVRFLRSWIEKPLHMGAVMPSGRILARTMAQYVDVASADPVIELGPGTGAITNALIEHGVDPKRLVLVEYDPGFCALLRDRYPQATVVQGDAYTLRDSLWNVLSARASAIVSGLPLVTKPMLTRLKLIRDAFATLAPGAPFVQFTYSVVPPIPKSLPGISTEASERIWMNLPPARVWVYRKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW80</accession>
    <name>RNPH_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease PH</fullName>
            <shortName evidence="1">RNase PH</shortName>
            <ecNumber evidence="1">2.7.7.56</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA nucleotidyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rph</name>
        <name type="ordered locus">Nwi_0193</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Phosphorolytic 3'-5' exoribonuclease that plays an important role in tRNA 3'-end maturation. Removes nucleotide residues following the 3'-CCA terminus of tRNAs; can also add nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates, but this may not be physiologically important. Probably plays a role in initiation of 16S rRNA degradation (leading to ribosome degradation) during starvation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>phosphate + tRNA(n+1) = a ribonucleoside 5'-diphosphate + tRNA(n)</text>
            <dbReference type="Rhea" id="RHEA:10628"/>
            <dbReference type="Rhea" id="RHEA-COMP:17343"/>
            <dbReference type="Rhea" id="RHEA-COMP:17344"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:173114"/>
            <dbReference type="EC" id="2.7.7.56"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexameric ring arranged as a trimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNase PH family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.56" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313529.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SW80"/>
    <dbReference type="SMR" id="Q3SW80"/>
    <dbReference type="STRING" id="323098.Nwi_0193"/>
    <dbReference type="EnsemblBacteria" id="ABA03461">
        <property type="protein sequence ID" value="ABA03461"/>
        <property type="gene ID" value="Nwi_0193"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0193"/>
    <dbReference type="eggNOG" id="COG0689">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050858_0_0_5"/>
    <dbReference type="OMA" id="KGKGQGW"/>
    <dbReference type="OrthoDB" id="1824064at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-exoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009022">
        <property type="term" value="F:tRNA nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11362">
        <property type="entry name" value="RNase_PH_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00564">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002381">
        <property type="entry name" value="RNase_PH_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018336">
        <property type="entry name" value="RNase_PH_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="SSF55666"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01966">
        <property type="entry name" value="RNasePH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01277">
        <property type="entry name" value="RIBONUCLEASE_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_1000024843" description="Ribonuclease PH">
        <location>
            <begin position="1"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <note>substrate</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="124"/>
            <end position="126"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <note>substrate</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00564"/>
        </source>
    </evidence>
    <sequence length="237" mass="25663" checksum="935E40CD40B64874" modified="2005-10-11" version="1">MRPSRRAPDELRAVSLERGVVKYAEGSCLVKFGDTHVLVTATLEERLPPWLKGQGRGWVTAEYGMLPRATLERTRREASAGKQGGRTVEIQRLIGRSLRAAVDLEALGERQITIDCDVIQADGGTRTASITGAWVALADCVNWMKARNMLKAGVLRGNVAAISCGIYNGTPVLDLDYAEDSEADTDANFVMTGEGRIIEVQGTAEKVPFSDDEFLALMALAKKGVARLVDLQKMAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWA3</accession>
    <name>Q3SWA3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0170"/>
    <dbReference type="EnsemblBacteria" id="ABA03438">
        <property type="protein sequence ID" value="ABA03438"/>
        <property type="gene ID" value="Nwi_0170"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0170"/>
    <dbReference type="HOGENOM" id="CLU_2383210_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="94" mass="10797" checksum="C53FE18363FB780C" modified="2005-10-11" version="1">MKLFIKSLGVIRAGRWPPGLSKGSRRSCVRALQLLTESEAELYFLDLTRFLHSNRCPSPDQARGHASFDNAIVIQRYFSRQSIRRNQDTMKAET</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWJ1</accession>
    <name>Q3SWJ1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminotransferase</fullName>
            <ecNumber evidence="1">2.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0082</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0082"/>
    <dbReference type="EnsemblBacteria" id="ABA03350">
        <property type="protein sequence ID" value="ABA03350"/>
        <property type="gene ID" value="Nwi_0082"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0082"/>
    <dbReference type="eggNOG" id="COG0436">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_4_5_5"/>
    <dbReference type="OMA" id="NDECYSE"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901605">
        <property type="term" value="P:alpha-amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="2">
        <location>
            <begin position="58"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="423" mass="45182" checksum="AA89A1E3F274B15C" modified="2005-10-11" version="1">MATPASSRAAQGDASPTQLDAGAFLSAAGPAALTAASERSPFARTAELLAPYQPEQSLINLSLGEPQDPPPDFVGPILSRHIDEFRRYPMARGIEPFRRAAAEWLSRRFALPRAIDPDAEILALNGSREGLFYAAIAAARYVAPRKGAPAILMPNPFYPAYGAGARAANCEAIFLPATRANGFLPDLDSLDDATLARTVAIYLASPANPQGAVASRDYLSRLIALARRHGFLVFSDECYSEIYTQSPPGSALECAGPDFAHVVAFQSLSKRSNLPGLRVGFVAGDRNFLTAFHELRNVAAPQVPAPLQHVGAAAYGDEAHVEENRRLYRLKFDLADGIIGTRFGYARPAGGFCLWLDVSAYGGDEAATIRLYREAGLRVVPGSYLARPQGDGGNPGAGYIRLALVADIETTAEALRRLVKTLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWJ3</accession>
    <name>Q3SWJ3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313417.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03348">
        <property type="protein sequence ID" value="ABA03348"/>
        <property type="gene ID" value="Nwi_0080"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0080"/>
    <dbReference type="HOGENOM" id="CLU_1813795_0_0_5"/>
    <dbReference type="OrthoDB" id="2010699at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="142" mass="16713" checksum="4EDA14522571D5B8" modified="2005-10-11" version="1">MSKFRDMLLEEREALLKVYRPLETDIKAMRFEMYEKQQRLAELASDIEKINLALKAVEDADKRPQITIMEAVVEVLKDRPEGLTALEILAEINTRYFGDRIIRSSLSPQLSRLKDRDHKIGLRGKKWFLLPQQPSLFVERRD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-12-15" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWM0</accession>
    <name>ACCD_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta</fullName>
            <shortName evidence="1">ACCase subunit beta</shortName>
            <shortName evidence="1">Acetyl-CoA carboxylase carboxyltransferase subunit beta</shortName>
            <ecNumber evidence="1">2.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">accD</name>
        <name type="ordered locus">Nwi_0053</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the acetyl coenzyme A carboxylase (ACC) complex. Biotin carboxylase (BC) catalyzes the carboxylation of biotin on its carrier protein (BCCP) and then the CO(2) group is transferred by the transcarboxylase to acetyl-CoA to form malonyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + N(6)-carboxybiotinyl-L-lysyl-[protein] = malonyl-CoA + N(6)-biotinyl-L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:54728"/>
            <dbReference type="Rhea" id="RHEA-COMP:10505"/>
            <dbReference type="Rhea" id="RHEA-COMP:10506"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:83144"/>
            <dbReference type="ChEBI" id="CHEBI:83145"/>
            <dbReference type="EC" id="2.1.3.15"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Acetyl-CoA carboxylase is a heterohexamer composed of biotin carboxyl carrier protein (AccB), biotin carboxylase (AccC) and two subunits each of ACCase subunit alpha (AccA) and ACCase subunit beta (AccD).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AccD/PCCB family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.15" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313392.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWM0"/>
    <dbReference type="SMR" id="Q3SWM0"/>
    <dbReference type="STRING" id="323098.Nwi_0053"/>
    <dbReference type="EnsemblBacteria" id="ABA03321">
        <property type="protein sequence ID" value="ABA03321"/>
        <property type="gene ID" value="Nwi_0053"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0053"/>
    <dbReference type="eggNOG" id="COG0777">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015486_1_0_5"/>
    <dbReference type="OMA" id="PEGLWIK"/>
    <dbReference type="OrthoDB" id="504557at2"/>
    <dbReference type="UniPathway" id="UPA00655">
        <property type="reaction ID" value="UER00711"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009317">
        <property type="term" value="C:acetyl-CoA carboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016743">
        <property type="term" value="F:carboxyl- or carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001295">
        <property type="term" value="P:malonyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01395">
        <property type="entry name" value="AcetylCoA_CT_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000438">
        <property type="entry name" value="Acetyl_CoA_COase_Trfase_b_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01070">
        <property type="entry name" value="ACCCTRFRASEB"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00515">
        <property type="entry name" value="accD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword id="KW-0276">Fatty acid metabolism</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000389803" description="Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta">
        <location>
            <begin position="1"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="2">
        <location>
            <begin position="24"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="282"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="289"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="319" mass="35183" checksum="5FF750C50052E5A2" modified="2005-10-11" version="1">MNWLTNVVRPKIRNILKRETPDNLWIKCPDTGQLVFYKDVESNQFVIPGSNYHMRMSADARLKSIFDNETWYDVALPEVTADPLKFRDERKYVDRIRDARAKTGLHDSVKVGFGRLEGSAVVVAVQDFDFMGGSLGMAAGEAIVRGLELAVEKRCPFIVFAASGGARMQEGVLSLMQLPRTTVAVQMLREARQPYIVVLTNPTTGGVTASYAMLGDVQIAEPGALIGFAGARVIEQTIREKLPDGFQRAEYLRDHGMIDLVVHRHELRPTLARVCRLLTKAPEMIEQEPEPSAPVPPDEPDEPAATQEAPPAAPAAPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMT8</accession>
    <name>Q3SMT8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Ferric uptake regulation protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">fur</name>
        <name evidence="6" type="ordered locus">Nwi_0013</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as a global negative controlling element, employing Fe(2+) as a cofactor to bind the operator of the repressed genes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the Fur family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0013"/>
    <dbReference type="EnsemblBacteria" id="ABA03281">
        <property type="protein sequence ID" value="ABA03281"/>
        <property type="gene ID" value="Nwi_0013"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0013"/>
    <dbReference type="eggNOG" id="COG0735">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096072_3_2_5"/>
    <dbReference type="OMA" id="YLYGVCT"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07153">
        <property type="entry name" value="Fur_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002481">
        <property type="entry name" value="FUR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043135">
        <property type="entry name" value="Fur_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33202">
        <property type="entry name" value="PTHR33202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01475">
        <property type="entry name" value="FUR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA03281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="156" mass="18213" checksum="692176E9675DD742" modified="2005-10-11" version="1">MRHRNRTLEMMALKSTPVQKPTTIEARCAAAGMRMTEQRRVIARVLAEASDHPDVEELYRRCAGVDDKISISTVYRTVKLFEDAGIIERHDFREGRARYETMRDSHHDHLINLRDGQVIEFTSDEIEKLQAEVARKLGYRLVDHRLELYCVPMDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMT9</accession>
    <name>Q3SMT9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ribosomal-protein-alanine acetyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.128</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0012</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.128" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313351.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0012"/>
    <dbReference type="EnsemblBacteria" id="ABA03280">
        <property type="protein sequence ID" value="ABA03280"/>
        <property type="gene ID" value="Nwi_0012"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0012"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_23_2_5"/>
    <dbReference type="OMA" id="ATLMDIC"/>
    <dbReference type="OrthoDB" id="1826274at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006474">
        <property type="term" value="P:N-terminal protein amino acid acetylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006464">
        <property type="entry name" value="AcTrfase_RimI/Ard1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01575">
        <property type="entry name" value="rimI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="13"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="160" mass="17991" checksum="5BC9A72C2F794D0A" modified="2005-10-11" version="1">MMTWFPGRERAVPIVEPASPNDAARLAAIHGASFHRGWGESEFETMIAERNTLVQRLRLRSNVIGFAVSRIAADEAEILSIAIDAGYRGQGFSRNLLLTHLGHLAGRGVRTVFLEVEENNQPARRLYERAGFAVVGRRERYYREAGDLELNALVMRRNLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMU1</accession>
    <name>Q3SMU1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF2778 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0010</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0010"/>
    <dbReference type="EnsemblBacteria" id="ABA03278">
        <property type="protein sequence ID" value="ABA03278"/>
        <property type="gene ID" value="Nwi_0010"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0010"/>
    <dbReference type="eggNOG" id="ENOG502ZBN2">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037312_0_0_5"/>
    <dbReference type="OMA" id="ACAWTLY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021225">
        <property type="entry name" value="Tlde1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10908">
        <property type="entry name" value="DUF2778"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2778" evidence="2">
        <location>
            <begin position="295"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="400" mass="42231" checksum="26E608B65B43220E" modified="2005-10-11" version="1">MTLDVGRMTYVTGRRYYEAAAHSADLPRKAVPQNILGFAGLACIGLACAWTLYTRVSGGFEPAELVTASLPPVVSGRSAEPVHRIAAAYEALEEIPGVRAPQAIAGVRAARFPVSKAPVSKNRAANAGVTKAGVTKARAEDPQARARTALLYGPRLLGPPPGTFEPAAASQIAVSSLAEAPPEILPLPQASQISSIVPMPAPRPSELRQLQAASAPSRSEIAQASAAVAPEPNKPSIFEKLFGRPAAGLKLAFAAPDGGVSSDGESLTLGSIPQHESTAIYDISARTVYLPDGTRLEAHSGLGKMMDDPRYADRKMRGVTPPHIYDLSLRERPFHGVEAIRLKPIGGEDKIYGRTGLLAHTYMLGPSGASNGCVSFKNYDAFLRAYKSGKIKRLVVVAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMY3</accession>
    <name>Q3SMY3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, XRE family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_3111</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316269.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3111"/>
    <dbReference type="EnsemblBacteria" id="ABA06358">
        <property type="protein sequence ID" value="ABA06358"/>
        <property type="gene ID" value="Nwi_3111"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3111"/>
    <dbReference type="eggNOG" id="COG5499">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125852_2_0_5"/>
    <dbReference type="OMA" id="RTISEAW"/>
    <dbReference type="OrthoDB" id="1684303at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039060">
        <property type="entry name" value="Antitox_HigA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40455">
        <property type="entry name" value="PTHR40455"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="62"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="124" mass="13731" checksum="6DB2A86DE96D639F" modified="2005-10-11" version="1">MKATLIVIQNQADHEAAKALVGKLIQSSDAADRARMVAQSRLIEAYERTRWPRKAPPLPDLLSYLVEQHGLTRADLVPLLGTASRVSEVMSGKRELSMTMVKRLRERFHIPADLLISASESVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN12</accession>
    <name>Q3SN12_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_3082</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3082"/>
    <dbReference type="EnsemblBacteria" id="ABA06329">
        <property type="protein sequence ID" value="ABA06329"/>
        <property type="gene ID" value="Nwi_3082"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3082"/>
    <dbReference type="eggNOG" id="COG0286">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_613474_0_0_5"/>
    <dbReference type="OMA" id="AYKIVEW"/>
    <dbReference type="OrthoDB" id="1649886at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026741">
        <property type="entry name" value="SNO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12706">
        <property type="entry name" value="PTHR12706"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="515" mass="54223" checksum="4F973B39D823E669" modified="2005-10-11" version="1">MNALFPAAEAVPAAPVSASVDPAACIHAAALLLPHIERGTRVDAAILRGVMTTAFGASDATGAWDWKRAYDACEAAIVLFLRKYGKALLRKAGSPARALPLLDKISDLLPTHTRRSEESQALQQFSTPAPLSLAVAIAAAITPADRVLEPSAGTGLLAILAEVAGGALVLNELAEMRAALLSFLFPAISVTRFDAAQIDDHLDAAVVPTVVLMNPPFSVMAHVEGRVADATFRHVASALARLAPGGRLVTITGAGFSQDNTVWAPAFTCLQERGRVVFSAAIDGSVYAKHGTTFATRLTVIDKLPADDPSVFPDAPGVAPDVATLHGWLTEHLPARLPVDSSIAVPIAAPVAPRTVRGYLNRAAKLALAASPAAPDGVPLAYDTVEWKPTENGRLSDSIYEDYGLQTIRIAGAQAHPMLRNDAPVLTDYDTVGIGLNLDRASDGAGRDRVFVVVEAYQTGLGDRRRHRVEAVEPAGIGNELGALGFEHVPDRLIGQFRMAMRLGVSDAFVEQPGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN23</accession>
    <name>Q3SN23_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Pyrimidine 5-nucleotidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_3070</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316234.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3070"/>
    <dbReference type="EnsemblBacteria" id="ABA06318">
        <property type="protein sequence ID" value="ABA06318"/>
        <property type="gene ID" value="Nwi_3070"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3070"/>
    <dbReference type="eggNOG" id="COG1011">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059493_2_1_5"/>
    <dbReference type="OMA" id="LYPHHVN"/>
    <dbReference type="OrthoDB" id="1167152at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02604">
        <property type="entry name" value="HAD_5NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010237">
        <property type="entry name" value="Pyr-5-nucltdase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01132">
        <property type="entry name" value="C1.5.3:_5'-Nucleotidase_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01993">
        <property type="entry name" value="Pyr-5-nucltdase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="238" mass="26838" checksum="15462DF9170D5654" modified="2005-10-11" version="1">MTCAQRHPHRGFSHIDTWVFDLDNTLYPHHVNLWQQVDARIGEYIGQFLNVDPVEARRIQKDYYKRFGTSMRGMMTEHGVSPDDYLAYVHQIDHSPLDPNPAMGAAIERLPGRKLILTNGSTAHAGKVLERLGFGHHFEAVFDIIAAGMEPKPSPRTYRRFLERHHVDAARSAMFEDLARNLVVPHELGMTTVLVVPDGTREVVREDWEMEGRGGAPHVDHVTDDLSGFLAGLTARSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN40</accession>
    <name>Q3SN40_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, TetR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_3052</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316217.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3052"/>
    <dbReference type="EnsemblBacteria" id="ABA06301">
        <property type="protein sequence ID" value="ABA06301"/>
        <property type="gene ID" value="Nwi_3052"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3052"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_28_1_5"/>
    <dbReference type="OMA" id="EIDGWFE"/>
    <dbReference type="OrthoDB" id="2047542at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011075">
        <property type="entry name" value="TetR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16925">
        <property type="entry name" value="TetR_C_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="3"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="183" mass="20930" checksum="2D97ABD6188082FC" modified="2005-10-11" version="1">MAKIERNEIITRAAELFRIGGYDDTSMAQIADACGIRKASLYHHFPEKDGLVLAAIEHIHEHFRKHIFAIAYRNNADARQRLHALCEATFSYFNGRDGGCLLGNFALALTERAPRFQEPLRSYFDDWTKAIAHLLTAEHGESRARDLAFDSVAHIQGAIMMMRLYHQPQHLRRALETSIRMLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN59</accession>
    <name>Q3SN59_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulator, Crp/Fnr family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_3032</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316200.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3032"/>
    <dbReference type="EnsemblBacteria" id="ABA06282">
        <property type="protein sequence ID" value="ABA06282"/>
        <property type="gene ID" value="Nwi_3032"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3032"/>
    <dbReference type="eggNOG" id="COG0664">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075053_3_4_5"/>
    <dbReference type="OMA" id="WVKIRTY"/>
    <dbReference type="OrthoDB" id="1596937at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012318">
        <property type="entry name" value="HTH_CRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13545">
        <property type="entry name" value="HTH_Crp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00419">
        <property type="entry name" value="HTH_CRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="SSF51206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51063">
        <property type="entry name" value="HTH_CRP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="16"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="HTH crp-type" evidence="3">
        <location>
            <begin position="150"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA06282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="224" mass="25082" checksum="80AD61E989999F90" modified="2005-10-11" version="1">MSRQSEFAVILKMNPLFSDLEPDDLQRMAALCHTVQVNAGEVLFQKGDDGDALYGVRRGQVRIETGDSDGGRLTLNFQGPGDLFGEIAVLDGQSRTADAVAGEPAELFVLRRGDLLAFLEREPRIAIRLIELLCQRIRWISERMEESVLQPLPVRMARRLCALADDYGSEVHISQEQLGVYVGAARESVNRQLQQWRKDGILDLQRGRILLKNISRLNAVARNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN62</accession>
    <name>Q3SN62_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Peptidase C14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_3029</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3029"/>
    <dbReference type="EnsemblBacteria" id="ABA06279">
        <property type="protein sequence ID" value="ABA06279"/>
        <property type="gene ID" value="Nwi_3029"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3029"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4249">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041791_0_0_5"/>
    <dbReference type="OMA" id="PVDAQIW"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029030">
        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001309">
        <property type="entry name" value="Pept_C14_p20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001440">
        <property type="entry name" value="TPR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="SSF52129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50208">
        <property type="entry name" value="CASPASE_P20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="1 2" id="KW-0802">TPR repeat</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229040" evidence="4">
        <location>
            <begin position="26"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="CASPASE_P20" evidence="5">
        <location>
            <begin position="30"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="382"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="450"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="261"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="276"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="308"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="331"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="348"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA06279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="527" mass="55662" checksum="0361D8F87A3A6666" modified="2005-10-11" version="1">MNIRVAVLAVLTLTMALAMGPAASAATDGSARVALVIGNAVYPDSEAPLKEPINDARDVAAELKRNGFDVETGENMTGDAMRLALKRLYTRIKPGSVALIFFSGYGIQSGRQSYLIPVDAQLWTEADVRRDGFSLETILGEINNRGAGVKIALLDASRRNPFERRFRSFSAGLAPVIAPSGSLVMYSAALSSVVGDVSADHSLFVSELLKEIRVPGLTAEQTLNRTRVGVTRASRAEQVPWISSSLAEDFYFVPGQGGQADAALMPSTPHRPQAQAPSAPVAPPPSASAPPASDPAASAAPAPAPATTPAESMAPPHTPAPSQTPAVASAPSEPAPSPAQVPEPAAEPRPQARDDHKTELALAEDPTIKSLTARIAAKTDDASALYRRGQVYASKGAYALAIKDFDATIRLNPKDVEAYNNRCWVRTVIGELQAARRDCDEALRLRRNFVDALDSRGLLNLKSGANKKAIADFDAALKINPRLTSSLYGRGLARQRSGAVAEGSLDIANAKAMDPDIAKEFAGYGVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SND8</accession>
    <name>Q3SND8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Glutamate synthase (NADH) large subunit</fullName>
            <ecNumber evidence="17">1.4.1.14</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">Nwi_2953</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="14">Amino-acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glutamate synthase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.14" evidence="17"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2953"/>
    <dbReference type="MEROPS" id="C44.003"/>
    <dbReference type="EnsemblBacteria" id="ABA06203">
        <property type="protein sequence ID" value="ABA06203"/>
        <property type="gene ID" value="Nwi_2953"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2953"/>
    <dbReference type="eggNOG" id="COG0067">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0069">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0070">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_8_2_5"/>
    <dbReference type="OMA" id="TVFRLQH"/>
    <dbReference type="UniPathway" id="UPA00863"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016040">
        <property type="term" value="F:glutamate synthase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00982">
        <property type="entry name" value="gltB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02808">
        <property type="entry name" value="GltS_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002489">
        <property type="entry name" value="Glu_synth_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036485">
        <property type="entry name" value="Glu_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006982">
        <property type="entry name" value="Glu_synth_centr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002932">
        <property type="entry name" value="Glu_synthdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00310">
        <property type="entry name" value="GATase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04898">
        <property type="entry name" value="Glu_syn_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01645">
        <property type="entry name" value="Glu_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01493">
        <property type="entry name" value="GXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69336">
        <property type="entry name" value="SSF69336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0288">FMN</keyword>
    <keyword evidence="12" id="KW-0314">Glutamate biosynthesis</keyword>
    <keyword evidence="8" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="16">
        <location>
            <begin position="69"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="961"/>
            <end position="984"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ABA06203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1596" mass="174578" checksum="30FC361295291876" modified="2005-10-11" version="1">MSHPGRERTEMSGPSFERETIGSHSPAAESAAKPLEAAGPHAGHEQTHEHTWRPPAEGFYDPALERDSCGVGFIANIKGRKSHQIVSDAISILCNLEHRGAVGADPRSGDGAGILVQIPHVFFARKAKELGFTLPKPGEYAVGALFMPRETAWREIIRSIIADQIKAEGLTLLGWREVPTDNSSLGETVKPTEPANMQVFIGRNGAAATEDEFERRLYILRKSISQAIFERRERGLSGYYPVSMSCRTVIYKGMFLADQLGAYYPDLHEPDFESALALVHQRFSTNTFPAWSLAHPYRMVAHNGEINTLRGNVNWMAARQASVHSDLYGKDISRLWPISYEGQSDTACFDNSLEFLVQGGYSLAHAVMMMIPEAWAGNPLMDEQRRAFYEYHAALMEPWDGPAALAFTDGRQIGATLDRNGLRPARYLVTRDDRIVMASEMGVLKIPEDQIVTKWRLQPGKMLLVDLEQGRLIPDDEIKATLANSHPYREWLGRTQIQVENLPDAPARATRSNLPLLDRQRAFGYSQEDINILMTPMASIGEEASGSMGNDTPLSALSDRPKQLFTYFKQNFAQVTNPPIDPIREELVMSLVSIIGPRPNLFDLEGLASTKRLEVRQPILTDADLEKIRSISDVAESHFVSRTLDITFEASVGPAGLEQVLDELCACAEAAVREGVNIIILSDRMMGVDRIPIPALLACAAVHHHLIRVGLRTSVGLVVESGEPREVHHFACLAGYGAEAINPYLAFETIIAMKERLPGSLDDKEIVKRYIKSIGKGLLKVMSKMGISTYQSYCGAQIFDAIGLKADFIAKYFAGTHSRIEGVGLAEIAEETVRRHADAFGDIQIYKSALDVGGEYAFRTRGEDHAWTAESVSNLQHAVRGNSQERYRAFAKILNEQSERLLTLRGLFRFKTAEDDQRKPVPLDGVEPAKEIVKRFATGAMSFGSISREAHTTLAIAMNRIGGKSNTGEGGEEADRFKPMPTGDSMRSAIKQVASGRFGVTTEYLVNSDMIQIKMAQGAKPGEGGQLPGHKVNATIARVRHSTPGVGLISPPPHHDIYSIEDLAQLIYDLKNVNPESLVSVKLVSEIGVGTVAAGVAKARADHVTIAGFEGGTGASPLTSIKHAGSPWEIGLAETHQTLVREKLRARVAVQVDGGFRTGRDVVIGALLGADEFGFATAPLIAAGCIMMRKCHLNTCPVGVATQDPVLRRRFTGQPEHVINYFFFVAEEVREIMAQLGYRTFDEMIGQVQMLDQTALVTHWKAKGLDFSKLFYRQKEAPGQKIHHAEAQNHHLDKVLDRKLIAQAKPALDRGAPVTIETGINNTDRSTGAMLSGTVAKIYGHAGLPLDTIQVHFKGTAGQAFGAWLGRGITFDLEGEGNDYVGKGLSGGRIIVRPPADSGIVPEESIIVGNTVMYGAIEGECYFRGIAGERFAVRNSGAVAVVEGAGDHCCEYMTGGIVVVLGRTGRNFAAGMSGGVAYVLDETGDFSKLCNLAMVDLEPVLSEEMINAGTYHHSGDLEAHGRVDVFQNLLESDVERLHVLVTRHARLAGSRRAADILANWKAYLPKFRKVMPVEYRRALKEMKANADAEPKIAIGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNF5</accession>
    <name>Q3SNF5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Heat shock protein Hsp20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2936</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316111.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2936"/>
    <dbReference type="EnsemblBacteria" id="ABA06186">
        <property type="protein sequence ID" value="ABA06186"/>
        <property type="gene ID" value="Nwi_2936"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2936"/>
    <dbReference type="eggNOG" id="COG0071">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046737_4_1_5"/>
    <dbReference type="OMA" id="PYNVERH"/>
    <dbReference type="OrthoDB" id="1523369at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06470">
        <property type="entry name" value="ACD_IbpA-B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037913">
        <property type="entry name" value="ACD_IbpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="SSF49764"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="4">
        <location>
            <begin position="29"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA06186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="150" mass="17067" checksum="6724E03B4F316BB9" modified="2005-10-11" version="1">MRAYDFAPLSRSTIGYDHLFDLFDAAQRTEGEDHYPPYNIERQSEDRYQISLSLAGFSPDEIAVTAEQNVLSIEGRKTEAANKDYLYRGISARPFKRTFNLADYVKVEGATFENGLLQIKLVREIPEAMKPRRIPIGVAPDPQIEKKRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNG0</accession>
    <name>Q3SNG0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nitrogen regulatory protein P-II</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2931</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">In nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP. P-II-UMP allows the deadenylation of glutamine synthetase (GS), thus activating the enzyme. Conversely, in nitrogen excess P-II is deuridylated and promotes the adenylation of GS. P-II indirectly controls the transcription of the GS gene (glnA). P-II prevents NR-II-catalyzed conversion of NR-I to NR-I-phosphate, the transcriptional activator of glnA. When P-II is uridylylated to P-II-UMP, these events are reversed.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotrimer.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316106.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2931"/>
    <dbReference type="EnsemblBacteria" id="ABA06181">
        <property type="protein sequence ID" value="ABA06181"/>
        <property type="gene ID" value="Nwi_2931"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2931"/>
    <dbReference type="eggNOG" id="COG0347">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_173795_0_0_5"/>
    <dbReference type="OMA" id="GFHEGHL"/>
    <dbReference type="OrthoDB" id="1864676at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002187">
        <property type="entry name" value="N-reg_PII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00543">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51343">
        <property type="entry name" value="PII_GLNB_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA06181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="119" mass="13079" checksum="0D2E26225174F4C9" modified="2005-10-11" version="1">MNAIKLHPLKKLEIILAGEHKQFATDLLSRAGVKGYTIVGNLSGKGNHGMYEGHLMFNEDDALIMIIAVVPKDLADALLDGFQPFFDAHSGVIFVSDIQVCRPTKFVAAYPYEMARSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNG6</accession>
    <name>Q3SNG6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1289</name>
        <name evidence="2" type="ordered locus">Nwi_2925</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="2">Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04550">
        <property type="protein sequence ID" value="ABA04550"/>
        <property type="gene ID" value="Nwi_1289"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06175">
        <property type="protein sequence ID" value="ABA06175"/>
        <property type="gene ID" value="Nwi_2925"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1289"/>
    <dbReference type="KEGG" id="nwi:Nwi_2925"/>
    <dbReference type="HOGENOM" id="CLU_055261_2_1_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025161">
        <property type="entry name" value="Put_IS4/IS5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13340">
        <property type="entry name" value="DUF4096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="124" mass="14286" checksum="D5DBB891CA91EB80" modified="2005-10-11" version="1">MGIKRYELSDHQWAKIAPLLPGKASDPGRTGSDNRLFVNGCLWVLRSGAHWCDLPERYGRWKTVHRRFSRWCHAGVWERVFSTLTADRDNQYLMLDSTIVRAHQQAATGKGGPRIRRWGVPEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNJ4</accession>
    <name>Q3SNJ4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PRC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2897</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316077.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2897"/>
    <dbReference type="EnsemblBacteria" id="ABA06147">
        <property type="protein sequence ID" value="ABA06147"/>
        <property type="gene ID" value="Nwi_2897"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2897"/>
    <dbReference type="eggNOG" id="COG3861">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091638_1_1_5"/>
    <dbReference type="OMA" id="ANQKVGT"/>
    <dbReference type="OrthoDB" id="1849143at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="SSF50346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229057" description="PRC domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="PRC" evidence="2">
        <location>
            <begin position="42"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="140" mass="14860" checksum="6D8E8E66A63E0FD2" modified="2005-10-11" version="1">MKALSIAAALLFVLPTASFAEMTAKTPSQAAAPIAATGDWSASKMSGVNIYNEANEKVGEISDVIINAQGQVKGVVIEAGGFLGMGTHYVLVAMDSLKFANKADKASTGQTSDTKREWFPDRAVLNVTKDQLKSTPEFKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNP1</accession>
    <name>Q3SNP1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Integrase, catalytic region</fullName>
            <ecNumber evidence="3">2.7.7.49</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2850</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06100">
        <property type="protein sequence ID" value="ABA06100"/>
        <property type="gene ID" value="Nwi_2850"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2850"/>
    <dbReference type="eggNOG" id="COG2801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027402_15_1_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="79"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="252"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="329" mass="37613" checksum="E902E40DC82C0737" modified="2005-10-11" version="1">MADLPTGPKDPRSTVLSSEEEAIIVAFRKHTLLPLDDCLYALQPTIPHLSRSSLHRCLHRHGISRLPEVEGDKPIRKTFRSYPIGYFHIDIAEVQTAEGKLRLFVAIDRTSKFAYAELHQEAGKMVAAQFLRNLIVAVPYAIHTVLTDNGIQFTNHARHKYAFHHIFDRVCDENGIEHRLTRINHPWTNGQVERMNRTIKDATVKRFHYDNHDQLRRHLQDFIKAYNFGRRLKTLKAPHTLRVHLQTMDFRARSIHHRSNPSNAGTEHLGHRRRRRPSPDRSSPLRPRIGVSGSCAASSACPRPICASFPRRSQASTCSMACCRTAPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNS3</accession>
    <name>Q3SNS3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2815</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316013.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2815"/>
    <dbReference type="EnsemblBacteria" id="ABA06068">
        <property type="protein sequence ID" value="ABA06068"/>
        <property type="gene ID" value="Nwi_2815"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2815"/>
    <dbReference type="eggNOG" id="COG5266">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079897_0_0_5"/>
    <dbReference type="OMA" id="PEAGMYW"/>
    <dbReference type="OrthoDB" id="1382281at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019613">
        <property type="entry name" value="DUF4198"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10670">
        <property type="entry name" value="DUF4198"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004228996" evidence="1">
        <location>
            <begin position="24"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="270" mass="29420" checksum="D3E446627F434ED7" modified="2005-10-11" version="1">MTNHLKLALIAATVSLLAPPAQAHRAWLLPSATVLSGSDLWVTVDGAISNDLFYFEHNPLPLDKLAIEGPDGKAVTPENLAKGRFRSTVDFKLAQPGTYKLSLTHQGLFASYKLDGENKRWRGKPDELAKAIPAKATDVKVTENRSRIETFVTSGKPSSDTLKPTGTGLEMIPVTHPNNLAVGEKATFRLVLDGKPAGNVAVEIVPGGIRYRDTLNDSKATTDETGTFSITFPEPGYYWMKASVQDDKSTIKDAQQRRSTYITTVEVLPQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-01-10" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNU3</accession>
    <name>LEU3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-isopropylmalate dehydrogenase</fullName>
            <ecNumber evidence="1">1.1.1.85</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">3-IPM-DH</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Beta-IPM dehydrogenase</fullName>
            <shortName evidence="1">IMDH</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">leuB</name>
        <name type="ordered locus">Nwi_2795</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:32271"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.85"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) or Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.85" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315993.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SNU3"/>
    <dbReference type="SMR" id="Q3SNU3"/>
    <dbReference type="STRING" id="323098.Nwi_2795"/>
    <dbReference type="PRIDE" id="Q3SNU3"/>
    <dbReference type="EnsemblBacteria" id="ABA06048">
        <property type="protein sequence ID" value="ABA06048"/>
        <property type="gene ID" value="Nwi_2795"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2795"/>
    <dbReference type="eggNOG" id="COG0473">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031953_0_3_5"/>
    <dbReference type="OMA" id="EYDLGAR"/>
    <dbReference type="OrthoDB" id="1551125at2"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00072"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003862">
        <property type="term" value="F:3-isopropylmalate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01033">
        <property type="entry name" value="LeuB_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004429">
        <property type="entry name" value="Isopropylmalate_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42979">
        <property type="entry name" value="PTHR42979"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00169">
        <property type="entry name" value="leuB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0432">Leucine biosynthesis</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000083713" description="3-isopropylmalate dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="77"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="290"/>
            <end position="302"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalysis" evidence="1">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalysis" evidence="1">
        <location>
            <position position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01033"/>
        </source>
    </evidence>
    <sequence length="370" mass="39965" checksum="C88E3A4BDBC3404D" modified="2005-10-11" version="1">MATYKLLLLPGDGIGPEVMAEVKRLVGWLDAQGIAKFETEEGLVGGASYDADKVSITDATMAKALAADAVLFGAVGGPKWDSVPYEVRPEAGLLRLRKDLALYANLRPAICYPALAESSSLKPEVVEGLDIMIVRELTGGVYFGEPKTVTDLGNGQKRAVDTQVYDTYEIERIARVAFDLARKRRNKLTSMEKRNVMKTGVLWHEVVTQVHQREYKDVALQHQLADSGGMQLVRWPKQFDVIVTDNLFGDMLSDIAAMLTGSLGMLPSASLGDTDPKTEKRRALYEPVHGSAPDIAGQGLANPIAMLASFGMALRYSFDRGDLADKLDAAIAAVLAKGLRTADLKSEGATVVSTSQMGEAIVKELEALHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP23</accession>
    <name>Q3SP23_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2715</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315914.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2715"/>
    <dbReference type="EnsemblBacteria" id="ABA05968">
        <property type="protein sequence ID" value="ABA05968"/>
        <property type="gene ID" value="Nwi_2715"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2715"/>
    <dbReference type="eggNOG" id="COG3916">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085044_0_0_5"/>
    <dbReference type="OMA" id="SEQRMMF"/>
    <dbReference type="OrthoDB" id="1281637at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="248" mass="28492" checksum="2BE8B0EBD06F7BE5" modified="2005-10-11" version="1">MSPAPQTIAKQTIVKPVERGIELLDRIDYRLVETPADKDIIYRLRYRAYLNEGAIESNSDRRISDRFDDMPNSWIFGAYLDGTLASSIRLSISSPQHPLTPSVDVFNDFLQSKVDRGKVMVDPTRFVAEPELAGRYPELPYLTLRLCFVACSFFDADLGLATVRPEHRSFYRRLFNHQPLSPPRLYPGLIKPICLMAVDFPASREMVFTRYPYLRSSYFERRMLFERRAGVSVRASVDSPFPSIVPKS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-04-29" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP51</accession>
    <name>RNH_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease H</fullName>
            <shortName evidence="1">RNase H</shortName>
            <ecNumber evidence="1">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnhA</name>
        <name type="ordered locus">Nwi_2687</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit. May bind a second metal ion at a regulatory site, or after substrate binding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNase H family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315886.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SP51"/>
    <dbReference type="SMR" id="Q3SP51"/>
    <dbReference type="STRING" id="323098.Nwi_2687"/>
    <dbReference type="EnsemblBacteria" id="ABA05940">
        <property type="protein sequence ID" value="ABA05940"/>
        <property type="gene ID" value="Nwi_2687"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2687"/>
    <dbReference type="eggNOG" id="COG0328">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030894_6_0_5"/>
    <dbReference type="OMA" id="MQEIEIF"/>
    <dbReference type="OrthoDB" id="1953872at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09278">
        <property type="entry name" value="RNase_HI_prokaryote_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00042">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022892">
        <property type="entry name" value="RNaseHI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00075">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000332633" description="Ribonuclease H">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="RNase H type-1" evidence="2">
        <location>
            <begin position="5"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00408"/>
        </source>
    </evidence>
    <sequence length="154" mass="17188" checksum="9F580F33BA635FF1" modified="2005-10-11" version="1">MNSPALPHVTIFTDGACSGNPGPGGWGAILRFGDIEKELKGGEPHTTNNRMELLAAISALEALKRPALVDLTTDSQYVRQGITAWIHGWKRNGWRTADRKPVKNVDLWQRLDAALQPHQVRWHWIKGHDGHSENERADQLAREGVAMAKLMRTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP76</accession>
    <name>Q3SP76_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Short-chain dehydrogenase/reductase SDR</fullName>
            <ecNumber evidence="2">1.1.1.184</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2662</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.184" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315865.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2662"/>
    <dbReference type="EnsemblBacteria" id="ABA05915">
        <property type="protein sequence ID" value="ABA05915"/>
        <property type="gene ID" value="Nwi_2662"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2662"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_4_1_5"/>
    <dbReference type="OMA" id="WEVANVI"/>
    <dbReference type="OrthoDB" id="1135906at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004090">
        <property type="term" value="F:carbonyl reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="286" mass="30547" checksum="CBDE16B5BE07E66F" modified="2005-10-11" version="1">MSSDHPAPPFATQKQPMPGTTARMEPRPDHGESSYRGSGRLRGLRAIITGGDSGIGRAVAIAFAREGADILIAYLNESEDAQEVRSLIEKEGRKAALVAGDISDPDHCRAIVRRAVDELGGLDIVVNNAAHQATFREIGDISDDEWRMTFEVNIHAMFYLTKAAVPHLKPGSAIINTTSINSDMPNPILLAYATTKGAIQNFTGGLAQMLAEKSIRVNAVAPGPVWTPLIPSTMPEEVVTDFGKQVPMKRPGQPAELATAYVMLADPLSSYTSGTTVAVTGGRPFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPD5</accession>
    <name>Q3SPD5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Methyltransferase</fullName>
            <ecNumber evidence="8">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_2603</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:15197"/>
            <dbReference type="Rhea" id="RHEA-COMP:12418"/>
            <dbReference type="Rhea" id="RHEA-COMP:12419"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90615"/>
            <dbReference type="ChEBI" id="CHEBI:90616"/>
            <dbReference type="EC" id="2.1.1.72"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the N(4)/N(6)-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="8"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315806.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2603"/>
    <dbReference type="REBASE" id="11322">
        <property type="entry name" value="M.NwiORF2603P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05856">
        <property type="protein sequence ID" value="ABA05856"/>
        <property type="gene ID" value="Nwi_2603"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2603"/>
    <dbReference type="eggNOG" id="COG2189">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024927_5_1_5"/>
    <dbReference type="OMA" id="WRKANPM"/>
    <dbReference type="OrthoDB" id="1487464at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009007">
        <property type="term" value="F:site-specific DNA-methyltransferase (adenine-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002941">
        <property type="entry name" value="DNA_methylase_N4/N6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040843">
        <property type="entry name" value="RAMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001091">
        <property type="entry name" value="RM_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01555">
        <property type="entry name" value="N6_N4_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18755">
        <property type="entry name" value="RAMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00508">
        <property type="entry name" value="S21N4MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N6_N4_Mtase" evidence="9">
        <location>
            <begin position="42"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="RAMA" evidence="10">
        <location>
            <begin position="281"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF18755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA05856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="376" mass="41490" checksum="40AAF3C35A50D522" modified="2005-10-11" version="1">MGVSRRGASARAPRTKFDSPVSRIIIGDCVAGMSKLQAGSADLIFADPPYNLQLKGDLKRPDESHVDAVNSDWDKFSSFSAYDEFTRAWLLACRRVMKPSATIWVIGSYHNIFRVGAIMQDLGFWVLNDIVWRKTNPMPNFRGRRFTNAHETMIWAARDENAKGYTFNYEALKAANEDVQARSDWLIPLCTGNERLKDEDGRKVHPTQKPEGLLARVLLSSSKPGDLVIDPFNGTGTTGAVAKRLGRNYIGFERDRTYAAAAEKRIAAVEPLPEATLAPFMTAREAPRVAFSELIERGMISPGAKLVDAKKRHGALVRADGAIMLGDKVGSIHRIGAVAQGAGACNGWTFWHIETSKGLKLIDELRAEIRSQMASD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPL9</accession>
    <name>Q3SPL9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, MarR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2519</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315723.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2519"/>
    <dbReference type="EnsemblBacteria" id="ABA05772">
        <property type="protein sequence ID" value="ABA05772"/>
        <property type="gene ID" value="Nwi_2519"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2519"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102087_0_0_5"/>
    <dbReference type="OMA" id="FFFAYRD"/>
    <dbReference type="OrthoDB" id="1744037at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023187">
        <property type="entry name" value="Tscrpt_reg_MarR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01117">
        <property type="entry name" value="HTH_MARR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="16"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="169" mass="19171" checksum="DA375AFB336E098C" modified="2005-10-11" version="1">MAGRPEAPDRRSEETRWDIIELLFFAYRDFVGDPDHVLEEFGFGRAHHRVMHFVHRYPSLTVADLLDVLRITKQSLGRVLKQLLDEGYIVQKAGAADRRQRLLFATPKGEALVAQLAGLQTDRIKRALRGLDASDAEVVRQFLLGMIDHDDPDKVLEAILRADGKMVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPQ0</accession>
    <name>Q3SPQ0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ErfK/YbiS/YcfS/YnhG</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2488</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YkuD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2488"/>
    <dbReference type="MEROPS" id="C82.003"/>
    <dbReference type="EnsemblBacteria" id="ABA05741">
        <property type="protein sequence ID" value="ABA05741"/>
        <property type="gene ID" value="Nwi_2488"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2488"/>
    <dbReference type="eggNOG" id="COG1376">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055763_0_0_5"/>
    <dbReference type="OMA" id="CIGMLND"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071972">
        <property type="term" value="F:peptidoglycan L,D-transpeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16913">
        <property type="entry name" value="YkuD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005490">
        <property type="entry name" value="LD_TPept_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038063">
        <property type="entry name" value="Transpep_catalytic_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03734">
        <property type="entry name" value="YkuD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141523">
        <property type="entry name" value="SSF141523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229224" evidence="5">
        <location>
            <begin position="25"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="YkuD" evidence="6">
        <location>
            <begin position="195"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="26"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="322"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="329"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="371" mass="39201" checksum="FA04B4C396FA039F" modified="2005-10-11" version="1">MIKRILTVFAAIAAGAGGISLASAQGYPPPSPIYSTPPSAYPGEDDRSGAVDALPNFDPADDQSMQRHNSVELPPPGPVMSPDDPRYGSPVSSPAYSNRAPSPGPVMSPDDPRYGQPGPPPYQYPTRAPETVASTGGSDAGLRPPAPVGADGRPAPMSALPPDEQPEVDQGGEKQLPERLRRKEVSFSTKEPAGTIVVDTSNTHLYYVLGNNRAIRYGVRVGRDGFTWAGVQKISRKAEWPDWHPPTEMIERQPYLPRFMAGGPGNPLGARAMYLGSTVYRIHGTNQPSTIGKFVSSGCIGMLNEDVSDLFDRVKVGTRVVVMPGGPPRTDTTASTPASSGAPAQQPPQTASLPGTQPTSVPPLPEPVTVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPR0</accession>
    <name>Q3SPR0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2478</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315684.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05731">
        <property type="protein sequence ID" value="ABA05731"/>
        <property type="gene ID" value="Nwi_2478"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2478"/>
    <dbReference type="eggNOG" id="ENOG503355X">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159800_1_1_5"/>
    <dbReference type="OMA" id="WLDDESF"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229114" evidence="1">
        <location>
            <begin position="28"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="100" mass="10114" checksum="9D07396108EC33C1" modified="2005-10-11" version="1">MKKAVFALLTAATVAAGSLAMAPQAEARGGGAVAAGLLGGLAAGAIIGGAIASTPPAYAVPAPVYAAPPPAYMDGPPCHMVRERFWDGYGWRFRRVQVCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPT6</accession>
    <name>Q3SPT6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, AsnC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2452</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2452"/>
    <dbReference type="EnsemblBacteria" id="ABA05705">
        <property type="protein sequence ID" value="ABA05705"/>
        <property type="gene ID" value="Nwi_2452"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2452"/>
    <dbReference type="eggNOG" id="COG1522">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_0_3_5"/>
    <dbReference type="OMA" id="TAFILME"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019885">
        <property type="entry name" value="Tscrpt_reg_HTH_AsnC-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00519">
        <property type="entry name" value="HTH_ASNC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="16"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="170" mass="18950" checksum="2866A90D4E923FF8" modified="2005-10-11" version="1">MQWAGCRDLDGVTKSLDQIDLKILSEIQADGRITNVELAKRVGISPPPCLRRVRTLEEEGYIQGYRGLLDPQRLGFEVTVFASVHLSSQADADLRAFEEFVRGEPLVRECWMLSGEVDFILKCVAPDMATFQDFVAHLTAAPHVRNVRTSLVLHSSKYAPAVPLELKMPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ12</accession>
    <name>Q3SQ12_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cold-shock DNA-binding protein family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2375</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315590.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2375"/>
    <dbReference type="EnsemblBacteria" id="ABA05629">
        <property type="protein sequence ID" value="ABA05629"/>
        <property type="gene ID" value="Nwi_2375"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2375"/>
    <dbReference type="eggNOG" id="COG1278">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117621_4_1_5"/>
    <dbReference type="OMA" id="DGQKVQF"/>
    <dbReference type="OrthoDB" id="2034748at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012156">
        <property type="entry name" value="Cold_shock_CspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019844">
        <property type="entry name" value="CSD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002599">
        <property type="entry name" value="Cold_shock_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00050">
        <property type="entry name" value="COLDSHOCK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00352">
        <property type="entry name" value="CSD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CSD" evidence="3">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="69" mass="7443" checksum="9C09800422D1B3B3" modified="2005-10-11" version="1">MTTGTVKWFNGQKGFGFIQPNDGGNDVFVHISAVERAGLSGLNEGQQVNFELKTDKMRGKVSAENLSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQD5</accession>
    <name>Q3SQD5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Short chain enoyl-CoA hydratase</fullName>
            <ecNumber evidence="3">4.2.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2252</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.17" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315474.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2252"/>
    <dbReference type="EnsemblBacteria" id="ABA05506">
        <property type="protein sequence ID" value="ABA05506"/>
        <property type="gene ID" value="Nwi_2252"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2252"/>
    <dbReference type="eggNOG" id="COG1024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_6_5"/>
    <dbReference type="OMA" id="MDMCLSA"/>
    <dbReference type="OrthoDB" id="1498685at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="259" mass="27768" checksum="4FD3EE094CEA4D71" modified="2005-10-11" version="1">MRTFEHIIIESKDQVGVIRLNRPKMLNALSFAVLREIAAAVDGFEADDGIGCILITGNEKAFAAGADIKEMQPKSFIDMLNEDFKAIGGDRIATCRKPTIAAVSGYALGGGCELAMMCDIIIASDNATFGQPEITLGTIPGIGGTQRLTRAIGKAKAMDLCLTGRMMDAAEAERSGLVSRVVPVDKLMDEAMAAAKKIASMSRPAAAMAKDAVNRAFETTLSEGMNVERDLFRATFALEDRAEGMAAFIEKRKPTNKNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQG2</accession>
    <name>Q3SQG2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Integrase, catalytic region</fullName>
            <ecNumber evidence="2">2.7.7.49</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2225</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315447.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05479">
        <property type="protein sequence ID" value="ABA05479"/>
        <property type="gene ID" value="Nwi_2225"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2225"/>
    <dbReference type="eggNOG" id="COG2801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027402_15_1_5"/>
    <dbReference type="OMA" id="HTACADQ"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13683">
        <property type="entry name" value="rve_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="127"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="316" mass="36529" checksum="586F9696407F0767" modified="2005-10-11" version="1">MGQVLHGSATTTEAIRRAIQNSEESLRALSKRYGINQKTVAKWKKRTSVADLPTGPKDPRSTALSSEEEAIIVAFRKHTLLPLDDCLYALQPTIPHLSRSSLHRCLHRHGISRLPEVEGDKPIRKTFRSYPIGYFHIDIAEVQTAEGKLRLFVAIDRTSKFAYAELHQEAGKMVAAQFLRNLIVAVPYAIHTVLTDNGIQFTNHARHKYAFHHIFDRVCDENGIEHRLTRINHPWTNGQVERMNRTIKDATVKRFHYDNHDQLRRHLQDFIKAYNFGRRLKTLKGLTPYEFICKRWTSEPDRFIIDPIHQMPGLNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQM3</accession>
    <name>Q3SQM3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glutathione-dependent formaldehyde-activating, GFA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2164</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Gfa family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315387.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2164"/>
    <dbReference type="EnsemblBacteria" id="ABA05418">
        <property type="protein sequence ID" value="ABA05418"/>
        <property type="gene ID" value="Nwi_2164"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2164"/>
    <dbReference type="eggNOG" id="COG3791">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055491_7_4_5"/>
    <dbReference type="OMA" id="CHCGRIA"/>
    <dbReference type="OrthoDB" id="1474484at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016846">
        <property type="term" value="F:carbon-sulfur lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006913">
        <property type="entry name" value="CENP-V/GFA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04828">
        <property type="entry name" value="GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="SSF51316"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51891">
        <property type="entry name" value="CENP_V_GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CENP-V/GFA" evidence="3">
        <location>
            <begin position="7"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="119" mass="13089" checksum="E1092B5459E303C7" modified="2005-10-11" version="1">MQDGKTYTGGCHCGQVRFECTTDLAMVTACNCSICTKKGLHITFLAPQSFQLRAGEDNLKEYLFNKQMIRHQLCIDCGVEVFARGKKPDGTEVVALNVSCIDGIELSRLTLTPIDGRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQS0</accession>
    <name>Q3SQS0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2116</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2116"/>
    <dbReference type="EnsemblBacteria" id="ABA05371">
        <property type="protein sequence ID" value="ABA05371"/>
        <property type="gene ID" value="Nwi_2116"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2116"/>
    <dbReference type="eggNOG" id="COG5425">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3063986_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009354">
        <property type="entry name" value="Usg"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06233">
        <property type="entry name" value="Usg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="53" mass="6195" checksum="AC815F7E01A24926" modified="2005-10-11" version="1">MAASREFLLQWHGYGLTTAEIHYHLPDHPAVLQLYVWQDYDTAPDFPDAPWLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQS5</accession>
    <name>Q3SQS5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Amylo-alpha-1,6-glucosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2111</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315339.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2111"/>
    <dbReference type="EnsemblBacteria" id="ABA05366">
        <property type="protein sequence ID" value="ABA05366"/>
        <property type="gene ID" value="Nwi_2111"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2111"/>
    <dbReference type="eggNOG" id="COG3408">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019216_1_0_5"/>
    <dbReference type="OMA" id="EIPHTPY"/>
    <dbReference type="OrthoDB" id="70058at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035396">
        <property type="entry name" value="Bac_rhamnosid6H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032856">
        <property type="entry name" value="GDE_N_bis"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17389">
        <property type="entry name" value="Bac_rhamnosid6H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14742">
        <property type="entry name" value="GDE_N_bis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GDE_N_bis" evidence="1">
        <location>
            <begin position="44"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Bac_rhamnosid6H" evidence="2">
        <location>
            <begin position="393"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="741" mass="82103" checksum="EE82668936BFD44E" modified="2005-10-11" version="1">MTTSSGGAGTVVHTAPLAGAGAAPLAQFFIPATASLQERRPRTLKHGDTFAVFDHNGDALSGPGSPEGLFHRDTRHLSHLYLTIEGTRPMLLSSTLRDDNATLTCDLTNPDIYDSNGRLSFSHDLVHIRRSRLLWNATCFERLVVRNFDERPRRLTIEIAFDADFADIFEVRGTRRERRGLIQSPEVSPSRVTLNYMGLDHRHRSTTLSFDPAPTRLTGRSALFTVAIAPRESRSLFLEIHCGSEGMTQHAPRRAFFSALRDARRALRSSSSRAASIATSNEIFNEAVRRSVSDLYMLATDTPEGPCPYAGIPWFSTVFGRDALITALQTLWLDPALARGVLLHLAAHQATSTDPLADAEPGKILHEVRVGEMAELGEVPFRRYYGSVDSTPLFVVLAGAYLDRTGDVVTIRRLWPHIEAALTWIEQYGDRDGDGFVEYHRQTSEGLINQGWKDSYDSVFHADGTFAKGPIALVEVQAYVYGAWCAAAKIAEQLGYVLRETECEARANRLREAYDAHFFDEELGTYALALDGDKRRCRVRTSNAGHALWTSVALPERAGPVVRSLMGGSSFCGWGVRTVASTEARYNPMSYHNGSVWPHDNALLAAGFARYGFRREAARIFEGLFAASSYIDLRRLPELFCGFPRQRTRGPTFYPVACTPQAWAAAAPLYMLQICLGLGFDPGERRITFEEPMMPAFLNEVLLRNLSIGDDTADVAVRRSGQQVVVDVLARRGPVRVLTTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQW9</accession>
    <name>Q3SQW9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2064</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315301.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05322">
        <property type="protein sequence ID" value="ABA05322"/>
        <property type="gene ID" value="Nwi_2064"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2064"/>
    <dbReference type="eggNOG" id="ENOG502ZMF7">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1711392_0_0_5"/>
    <dbReference type="OMA" id="VEGDWGL"/>
    <dbReference type="OrthoDB" id="1736671at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229067" evidence="2">
        <location>
            <begin position="20"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="148" mass="15778" checksum="E87707C1879F65F9" modified="2005-10-11" version="1">MRIGLVLAVLIGTSLTAVAGSGPVIVIPGRPGVPVIINGVDASYAVVEGDWGLGRRMQPRPTVYGGRYVDDDPYVGHYYPSLGRSPGYGRLEIEPPPNRKPPRRAESFHQSWSARSNPTPAQSDIPQMPPPVILAPRIGPSGLPPGVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR48</accession>
    <name>Q3SR48_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Carboxysome shell carbonic anhydrase</fullName>
            <ecNumber evidence="3">4.2.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Carbonic anhydrase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Carboxysome shell protein CsoS3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">Nwi_1984</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + hydrogencarbonate = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Carboxysome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the beta-class carbonic anhydrase family. CsoSCA subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315230.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1984"/>
    <dbReference type="EnsemblBacteria" id="ABA05243">
        <property type="protein sequence ID" value="ABA05243"/>
        <property type="gene ID" value="Nwi_1984"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1984"/>
    <dbReference type="eggNOG" id="ENOG502Z9V7">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_535194_0_0_5"/>
    <dbReference type="OMA" id="VENSFCC"/>
    <dbReference type="OrthoDB" id="300636at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031470">
        <property type="term" value="C:carboxysome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015977">
        <property type="term" value="P:carbon fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014074">
        <property type="entry name" value="Carboxysome_shell_carb_anhy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043066">
        <property type="entry name" value="Carboxysome_shell_carb_anhy_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043065">
        <property type="entry name" value="Carboxysome_shell_carb_anhy_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08936">
        <property type="entry name" value="CsoSCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02701">
        <property type="entry name" value="shell_carb_anhy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1283">Bacterial microcompartment</keyword>
    <keyword evidence="7" id="KW-0120">Carbon dioxide fixation</keyword>
    <keyword evidence="9" id="KW-1282">Carboxysome</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="244"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ABA05243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="508" mass="56147" checksum="2013E2B972AFAC74" modified="2005-10-11" version="1">MNTRKRLQALRREANPVAAAAPQVVAILDHPACMIGSGQSCEHALVDRDLNRLLFDYERTVRSRFTRIVDVLKRISTHQHDENFIERAQQLAREQLGFELPPQVLEDAWVCGLDLSALHSRCIFSALKSSVENAPAEQVGWRQRLPLDENFLHSCGYHTVDISPCSDGRLQGVSPYVLRILPGPNVRVKAYAGALFDIEVDVSDWARREVERLAGAMVDGERLNYLKIAVYHFSSSSPGGQGCAAHGSNDRQATEAALQRLQNELRTAIDQTFGLGAAPDVLLIGVDTDLDALRIHLPDGFGELNAHRYFETAQVYRDTLGLAPEAARARIAGIVADAEGMGGWGQGNGRMHEGMRRLVLALAEANLSQIEYVIKHHTGRYKIIGHDEECIVAGDAVRSLQLRNLFYFARLDTIEEGAPDMDVGIKIFGNLNVAHGLPVPVLVHFEYDARIPESRARAIARGRRVRDAIEARYPDLAARGLLNCAIAVSERFGDESCVFVVDDAVDDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR81</accession>
    <name>Q3SR81_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative phage-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1950</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315201.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1950"/>
    <dbReference type="EnsemblBacteria" id="ABA05210">
        <property type="protein sequence ID" value="ABA05210"/>
        <property type="gene ID" value="Nwi_1950"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1950"/>
    <dbReference type="eggNOG" id="ENOG5032ZWD">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132664_0_0_5"/>
    <dbReference type="OMA" id="CEGCEGC"/>
    <dbReference type="OrthoDB" id="1362093at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="133" mass="14957" checksum="FC97EAAACDB1FBB6" modified="2005-10-11" version="1">MTGSSDTIRVVIPLTIRKRNGRPKILPPDDVTARDGRSQDPHVLRAIARAWSWRRQLESGAASTIQDIAAAEKISDRFISRMMRLAYLSPEVLDHLVIRRVSPALSLNNLVAVADRPWAEQMDMVFEGNVEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR91</accession>
    <name>Q3SR91_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">pre-crRNA processing endonuclease</fullName>
            <ecNumber evidence="2">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1940</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISPR-associated protein Cas5 family. Subtype I-C/Dvulg subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315193.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1940"/>
    <dbReference type="EnsemblBacteria" id="ABA05200">
        <property type="protein sequence ID" value="ABA05200"/>
        <property type="gene ID" value="Nwi_1940"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1940"/>
    <dbReference type="eggNOG" id="ENOG502Z82V">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086014_0_0_5"/>
    <dbReference type="OMA" id="YLGCREF"/>
    <dbReference type="OrthoDB" id="1619114at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021124">
        <property type="entry name" value="CRISPR-assoc_prot_Cas5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013422">
        <property type="entry name" value="CRISPR-assoc_prot_Cas5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010155">
        <property type="entry name" value="CRISPR-assoc_prot_Cas5d"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09704">
        <property type="entry name" value="Cas_Cas5d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029950">
        <property type="entry name" value="Cas_CT1134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01876">
        <property type="entry name" value="cas_Cas5d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02593">
        <property type="entry name" value="CRISPR_cas5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR029950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="230" mass="25956" checksum="54C19A52FFC0DF6E" modified="2005-10-11" version="1">MTYGIRLKVWGDYACFTRPEMKVERVSYDVITPSAARGILEAIHWKPAIRWVIDAIHVLERPRFQSIRRNEVGSKAPAGKIKSAMKRGDLEGLQLLVDEGRQQRASTVLVKPAYVIVARFELTGRGEEGDNEGKHLDIFNRRARRGQCFHQPCLGTREFAASFTLLDAGEPTPEPREQVGDLGFGNPRDLGFMLWDIDHEAGGKPSMFFRAELRDGVMKVPQPGSPEIRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRA8</accession>
    <name>RPOZ_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit omega</fullName>
            <shortName evidence="1">RNAP omega subunit</shortName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">RNA polymerase omega subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Transcriptase subunit omega</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpoZ</name>
        <name type="ordered locus">Nwi_1923</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Promotes RNA polymerase assembly. Latches the N- and C-terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA polymerase subunit omega family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315179.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRA8"/>
    <dbReference type="SMR" id="Q3SRA8"/>
    <dbReference type="STRING" id="323098.Nwi_1923"/>
    <dbReference type="EnsemblBacteria" id="ABA05183">
        <property type="protein sequence ID" value="ABA05183"/>
        <property type="gene ID" value="Nwi_1923"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1923"/>
    <dbReference type="eggNOG" id="COG1758">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125406_2_0_5"/>
    <dbReference type="OMA" id="EVDEPEM"/>
    <dbReference type="OrthoDB" id="1864134at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00366">
        <property type="entry name" value="RNApol_bact_RpoZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003716">
        <property type="entry name" value="DNA-dir_RNA_pol_omega"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006110">
        <property type="entry name" value="Pol_omega/Rpo6/RPB6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036161">
        <property type="entry name" value="RPB6/omega-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34476">
        <property type="entry name" value="PTHR34476"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01192">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01409">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63562">
        <property type="entry name" value="SSF63562"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00690">
        <property type="entry name" value="rpoZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000237478" description="DNA-directed RNA polymerase subunit omega">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="130" mass="14401" checksum="BD0638E406C4C06A" modified="2005-10-11" version="1">MARVTVEDCIDKVDNRFDLVLLAAHRARMISSGSQLTIDRDNDKNPVVALREIAEQTISPEDMREELVHSLQKFVEVDEPESDTVPLIGSAGASVDADDTEVAVERMTEEELLKGLEGLAPPEEQPEEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRC6</accession>
    <name>Q3SRC6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_1905</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05165">
        <property type="protein sequence ID" value="ABA05165"/>
        <property type="gene ID" value="Nwi_1905"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1905"/>
    <dbReference type="eggNOG" id="COG0628">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031275_1_0_5"/>
    <dbReference type="OMA" id="VEGFMRG"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002549">
        <property type="entry name" value="AI-2E-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716">
        <property type="entry name" value="PTHR21716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01594">
        <property type="entry name" value="AI-2E_transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="251"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="286"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="338"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="139"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA05165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="379" mass="39729" checksum="FC26FD03312C8F5C" modified="2005-10-11" version="1">MRFSVDGLPESQGFERQSLARSIAAGGIGIIAFGAALALAWYSSATLLMLFAGVLFGVFLTAMADLLRRVFGGNQTLRLVIVCILFTGLLSGVVVLGGATIAQQATALSSTLRAQLGNARDFLEKQGVDTSFLNVGARTPSTDNGVTPIPAPSASPSRGPKLPDASTIASGTGAIVSQTIKILARVFEGVGNFFIVIFLGLLLAAQPKVYRDGILRFVPRQRLPEAASLIDDVSETLRRWLLGQMATMASLFLLTWIGLTLIGIPGALVLGFITGLLTFIPNIGAILAGALIVLASLGSGLTAIISAFMLYLAVQFLEGNVLTPLIQRHAISIPPATLFAAQIFLGVLFGVWGLALALPLIAVIKVVLNHVWPDQTAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRF5</accession>
    <name>Q3SRF5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">NADH dehydrogenase subunit L</fullName>
            <ecNumber evidence="10">1.6.5.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_1876</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Membrane</location>
            <topology evidence="1 5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.6.5.3" evidence="10"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315132.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1876"/>
    <dbReference type="EnsemblBacteria" id="ABA05136">
        <property type="protein sequence ID" value="ABA05136"/>
        <property type="gene ID" value="Nwi_1876"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1876"/>
    <dbReference type="eggNOG" id="COG1009">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007100_6_0_5"/>
    <dbReference type="OMA" id="LIGFWQH"/>
    <dbReference type="OrthoDB" id="1274678at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018393">
        <property type="entry name" value="NADHpl_OxRdtase_5_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_mem"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003945">
        <property type="entry name" value="NU5C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001516">
        <property type="entry name" value="Proton_antipo_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42829">
        <property type="entry name" value="PTHR42829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00662">
        <property type="entry name" value="Proton_antipo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01974">
        <property type="entry name" value="NDH_I_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="161"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="215"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="257"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="301"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="331"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="359"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="387"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="428"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="464"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="509"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="563"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="664"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="domain" description="Proton_antipo_N" evidence="9">
        <location>
            <begin position="112"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Proton_antipo_M" evidence="8">
        <location>
            <begin position="178"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="33"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="33"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA05136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="688" mass="75840" checksum="233217AAFCADECA2" modified="2005-10-11" version="1">MIEAIVFLPLIGAILAGLIALLGAHWRNPSGDTVDHHDGHGHAHAAAAHDDHGHGDSRGHDDHHVSEPPAAGSRAAELITTTLLIASAVLSWITLVDVGFMHHDARIALFSWISSGDLQIGWSLRVDTLTAVMLVVVTTVSSLVHFYSIGYMDEDPHRPRFFGYLSLFTFAMLMLVTSDNLVQLFFGWEGVGLASYLLIGFWYQKPSANAAAIKAFVVNRVGDFGFSLGIFAVFMLVGSTDFDTIFSAAPGLTGKTIHFLAWDVNALTLICLLLFMGAMGKSAQLLLHTWLPDAMEGPTPVSALIHAATMVTAGVFMVARLSPLFELSPDAQAFVMFIGATTAIFAATVGLVQNDIKRIVAYSTCSQLGYMFVAMGAGAYTVGMFHLFTHAFFKALLFLGSGSVIYAMHHEQDIRNMGGLKDRIPYTYFVMVIGTLALTGFPLTAGYFSKDAIIESAYVSHNPFAYYGFLMTVAAAGLTSFYSWRLIFKTFHGKPHDQAHYKAAHESPIWMLIPIGVLAAGSILAGFPFKELFTGHGVEEFFRESVKMHPHILEEMHHIPKTIAFLPTVMMIMGFAVAWLFYIRRPDIPVQLAREHQMLYKFLLNKWYFDELYEVIFVRPAKWLGRFLWKKGDGLVIDGFGPDGVSSGVLDVTRNVVRLQTGYLYHYAFAMLIGVAGLITWFMFSGGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRJ7</accession>
    <name>Q3SRJ7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Protein-export membrane protein SecG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Nwi_1834</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in protein export. Participates in an early event of protein translocation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cell membrane</location>
            <topology evidence="2 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the SecG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1834"/>
    <dbReference type="EnsemblBacteria" id="ABA05094">
        <property type="protein sequence ID" value="ABA05094"/>
        <property type="gene ID" value="Nwi_1834"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1834"/>
    <dbReference type="eggNOG" id="COG1314">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094156_5_2_5"/>
    <dbReference type="OMA" id="VIHLMIV"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015450">
        <property type="term" value="F:protein-transporting ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004692">
        <property type="entry name" value="SecG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34182">
        <property type="entry name" value="PTHR34182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03840">
        <property type="entry name" value="SecG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01651">
        <property type="entry name" value="SECGEXPORT"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00810">
        <property type="entry name" value="secG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0811">Translocation</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="93"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="94"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA05094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="138" mass="13938" checksum="3836A3052190A746" modified="2005-10-11" version="1">MSGRPARTCDGRPEMQTAVIVIHLIIVATLTVVVLLQKSEGGGLGMGGGAGFMSSRGTANLLTRTTSFLAAGFFVTSLLLSWLAGVEQKPGSILDTAPASQSQPATPVAPPSGGGLLDSLKQSEQAPAPAGPQAPRSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRM8</accession>
    <name>Q3SRM8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UPF0056 inner membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_1802</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell inner membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the UPF0056 (MarC) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315059.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05063">
        <property type="protein sequence ID" value="ABA05063"/>
        <property type="gene ID" value="Nwi_1802"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1802"/>
    <dbReference type="eggNOG" id="COG2095">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079909_0_0_5"/>
    <dbReference type="OMA" id="MMAIVML"/>
    <dbReference type="OrthoDB" id="1323133at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002771">
        <property type="entry name" value="Multi_antbiot-R_MarC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33508">
        <property type="entry name" value="PTHR33508"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01914">
        <property type="entry name" value="MarC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00427">
        <property type="entry name" value="TIGR00427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA05063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="207" mass="21072" checksum="42AA728CF2226ADA" modified="2005-10-11" version="1">MLPDYALTTFATLMVVVDPIGLAPVFLSITQGLPKAARRGIGWRASAIAAAILVATALGGTWLLARLGIGLPAFRISGGLLLFVVAFEMVLGVRPAREAQQATQAAEEHARNIAAFPLAIPMIAGPGAITATLLLAGRAADQPVQLIALIAIIIAVAAICGVTFALAGRVAGALGHTGNVVLSRLLGVVLAALAVQFVIDGIREAFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRM9</accession>
    <name>Q3SRM9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA gyrase subunit A</fullName>
            <ecNumber evidence="8">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">gyrA</name>
        <name evidence="10" type="ordered locus">Nwi_1801</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the topoisomerase GyrA/ParC subunit family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type II topoisomerase GyrA/ParC subunit family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="8"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315058.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1801"/>
    <dbReference type="EnsemblBacteria" id="ABA05062">
        <property type="protein sequence ID" value="ABA05062"/>
        <property type="gene ID" value="Nwi_1801"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1801"/>
    <dbReference type="eggNOG" id="COG0188">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002977_6_1_5"/>
    <dbReference type="OMA" id="THHWLLF"/>
    <dbReference type="OrthoDB" id="217468at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034335">
        <property type="term" value="F:DNA negative supercoiling activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00187">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.268.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.199.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01897">
        <property type="entry name" value="GyrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005743">
        <property type="entry name" value="GyrA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006691">
        <property type="entry name" value="GyrA/parC_rep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035516">
        <property type="entry name" value="Gyrase/topoIV_suA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013758">
        <property type="entry name" value="Topo_IIA_A/C_ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013757">
        <property type="entry name" value="Topo_IIA_A_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002205">
        <property type="entry name" value="Topo_IIA_dom_A"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03989">
        <property type="entry name" value="DNA_gyraseA_C"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00521">
        <property type="entry name" value="DNA_topoisoIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00434">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101904">
        <property type="entry name" value="SSF101904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="SSF56719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 8 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="TOP4c" evidence="9">
        <location>
            <begin position="21"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GyrA-box" evidence="8">
        <location>
            <begin position="561"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="8">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA05062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="913" mass="100698" checksum="DB6E62C57057B28F" modified="2005-10-11" version="1">MSDIEDTKPEEPPAPSGIRPVSILEEMKRSYLDYAMSVIVARALPDARDGLKPVHRRILYAMYENGFEWNKPYRKSARTVGDVIGKYHPHGDQSVYDALVRMAQDFSMRVPLIDGQGNFGSVDGDMAAAMRYTESRLTKIAQSLLDDIDKDTVDFQPNYDNSEREPSVLPAKFPNLLVNGAGGIAVGMATNIPPHNLGEVIDACVALIDDPALGIDDLIKIIPGPDFPTGGIILGRQGIRSAYHLGRGSIVMRGKVSIDTIRKDREAIVVSEIPYQVNKASMVERIAELVREKKIDGISDLRDESDREGFRVVVELKRDAVPEIVLNQLYKYTPLQTNFGANMVALDAGRPQVMNLKDLLTLFIAFREQVVTRRTKFLLNKARDRAHILVGLAIAVANIDEIIRVIRESPDPNAARETLMSRDWPAKDVAAMITLIDDPRHRVSPDGTARLSMEQAKAILDLRLQRLTALGREEISEELDKLAIEIADYLDILRSRARVQAIIKTELGAVKSEFATPRRTVIVEQEGEVEDEDLIQREDMVVTVSHAGYVKRVPLSTYRAQKRGGKGRSGMQTRDEDFVSRLFVASTHTPVLFFSSRGQVYKEKVWRLPMAAPNARGKALINILPLDQGERITTIMPLPEDESSWADLDVMFATTSGTVRRNKLSDFVDVRRSGIIAMKLGEGESIVDVQICTERDDVLLTAAGGQCIRFPVTDVRVFSGRTSMGVRGIALAKGDRVISLSILHHFDATPAERAAYLKQSGAIRRAATGEDIEPEEATPEADEAAHTDLSLPEGRYVEMGAAEQFVLTVTTYGYGKRSSSFEFRVSGRGGKGIRATDPTKLGEIGHLVAGFPVEMSDQIMLVSDGGQLIRVPVEGIRITSRASKGVRVFNTADGEHVVSVEHIREDGENGNGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRP0</accession>
    <name>Q3SRP0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HIG1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1790</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1790"/>
    <dbReference type="EnsemblBacteria" id="ABA05051">
        <property type="protein sequence ID" value="ABA05051"/>
        <property type="gene ID" value="Nwi_1790"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1790"/>
    <dbReference type="HOGENOM" id="CLU_186083_2_0_5"/>
    <dbReference type="OMA" id="ATIWAMG"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007667">
        <property type="entry name" value="Hypoxia_induced_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04588">
        <property type="entry name" value="HIG_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51503">
        <property type="entry name" value="HIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="HIG1" evidence="2">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="53" mass="5956" checksum="491B41C57AC4C1C3" modified="2005-10-11" version="1">MASVAAVLILGLVNMIRGGNPWRSQYLMQTRVVLQFVAIVMVMMAVWSMMGRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRP5</accession>
    <name>Q3SRP5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1785</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315042.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1785"/>
    <dbReference type="EnsemblBacteria" id="ABA05046">
        <property type="protein sequence ID" value="ABA05046"/>
        <property type="gene ID" value="Nwi_1785"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1785"/>
    <dbReference type="eggNOG" id="COG3170">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_586186_0_0_5"/>
    <dbReference type="OMA" id="QPIIMIK"/>
    <dbReference type="OrthoDB" id="1398099at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="125"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="192"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="248"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="368"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="83"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="256"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="402"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="422" mass="43675" checksum="233FF5E397948F21" modified="2005-10-11" version="1">MAKNQNGLAESTGADESSGRPAGFLADEDGFDRRTIWRLGSWGVGSIGAVIVALLAYNSGAALRSRPLAGADLAKPSPQLEIAQAAERKIERLSSAIDTLNSDRERLFARLAVLEQGFESVTGSISRKASQTDAIATTSTPAASEPYPVREPDSPGQDALTAANASTDAPLSAPAAFITTAAAAAVKPPIAASSPVAASPPAIAAHDPTATMPGGQGETVTLGTAKSELEKNLHHHKEAQIVAASIPPIADAPQPPSPAQASSSETVKDTSPVPVPRTEFGIDLGSAASIKGLRTLWQSLLKSEPKELTSLHPIIVLKETSNGNGIQMRLVAGPLTDAATAARICAVLSEDGQTCGTTVFDGQRLAMNEDGKDSAVTPATKKPKTPPASRRRPARKSTRVEEPAQPPPPPPRPAAFSPFFSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRR4</accession>
    <name>Q3SRR4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Squalene/phytoene synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1766</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315023.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1766"/>
    <dbReference type="EnsemblBacteria" id="ABA05027">
        <property type="protein sequence ID" value="ABA05027"/>
        <property type="gene ID" value="Nwi_1766"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1766"/>
    <dbReference type="eggNOG" id="COG1562">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037269_6_1_5"/>
    <dbReference type="OMA" id="RKFWLAW"/>
    <dbReference type="OrthoDB" id="1449625at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002060">
        <property type="entry name" value="Squ/phyt_synthse"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00494">
        <property type="entry name" value="SQS_PSY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="SSF48576"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="286" mass="31743" checksum="FE610173E9D41D30" modified="2005-10-11" version="1">MNDTTRNTSAAFCAELVRTHDFGNYATTLFAPLRSRRALLALYAFNVEIVRVREQITQPLAGEVRLQWWMDMLAGAGHGGVEGNPVAAELLLAIREHDLPVALLQRLIEVHQFDLYNDPMPDIAALEQYLADTSCALFSLSSRTIGQRPDETDHLARHAGLACGMARVIANFGFYASRRQSYVPLKLLEQHGVDLEQMFTGRDAPSVRAAIRGLAEEARSHLKVALGLLSDLPPDSRGVYLPLALVRRALDDVGGAGINPFMPRPISRLRVLWTMWRASRSKAFRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS03</accession>
    <name>Q3SS03_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Lipopolysaccharide export system permease protein LptF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_1677</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314937.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1677"/>
    <dbReference type="EnsemblBacteria" id="ABA04938">
        <property type="protein sequence ID" value="ABA04938"/>
        <property type="gene ID" value="Nwi_1677"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1677"/>
    <dbReference type="eggNOG" id="COG0795">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028799_7_0_5"/>
    <dbReference type="OMA" id="NYISSMI"/>
    <dbReference type="OrthoDB" id="1558251at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030922">
        <property type="entry name" value="LptF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005495">
        <property type="entry name" value="LptG/LptF_permease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33529">
        <property type="entry name" value="PTHR33529"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03739">
        <property type="entry name" value="LptF_LptG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04407">
        <property type="entry name" value="LptF_YjgP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="45"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="279"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="308"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA04938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="390" mass="43221" checksum="7B010D2C03666382" modified="2005-10-11" version="1">MGSIDKYIFRTTLASFVVVLVSLTGVIWITQALRGIDLMTSQGQTILTFLGITSLAIPVLVLIISPIALMIAVSHTLHKLATDSEIIVMNAAGLSPFRLFRPFLYATVVVALLVAFIAAYLAPDGMRRLKQWDAEITADVLANVLQPGRFAQLDPNLTIRIRERQPGGQLAGIFIDDRRNSNERLSIIADHGTVMKNDQGSFLILEDGHLERFETGKREPAMVAFDRYAFDMSKFSNRGHDVVYGIRERYLWELIAPDEKDPLVQQLRGQFRAELHDRFTAPIYPFAFAALTFAFLGSPRTTRQSRNFSMGAAIAAVFGLRMAGFALSVMTVKSPMAALLQYLLLFGAIGISLRVIIAGIVLEPPAALMEAINRSNARILRLLRKRPAFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS41</accession>
    <name>Q3SS41_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1639</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04900">
        <property type="protein sequence ID" value="ABA04900"/>
        <property type="gene ID" value="Nwi_1639"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1639"/>
    <dbReference type="eggNOG" id="ENOG5030WPW">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2302944_0_0_5"/>
    <dbReference type="OMA" id="NSAPCNG"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="100" mass="11756" checksum="B4B958A9DB7E3F1D" modified="2005-10-11" version="1">MADNDSTIAWHRAQLKKHRETLRDMEVRRFRFGETADPRVRVETLRMAANLRRKITASEKVIGAYEKRTRRPRTTDFRSLANVQWGNWNSAPCNGEIARD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS80</accession>
    <name>Q3SS80_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1600</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1600"/>
    <dbReference type="EnsemblBacteria" id="ABA04861">
        <property type="protein sequence ID" value="ABA04861"/>
        <property type="gene ID" value="Nwi_1600"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1600"/>
    <dbReference type="eggNOG" id="COG0861">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070543_0_0_5"/>
    <dbReference type="OMA" id="KAIFWGS"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005496">
        <property type="entry name" value="Integral_membrane_TerC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022301">
        <property type="entry name" value="Integral_membrane_YjbE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03741">
        <property type="entry name" value="TerC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03717">
        <property type="entry name" value="R_switched_YjbE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="267"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="292" mass="30416" checksum="B6BB7161BFA33363" modified="2005-10-11" version="1">MLRAGTLSAESGGVRSKEQVLDSLLHVFDLTAIASAFDQFRNDLNDPAFWLAVSKIIWIDVVLSGDNALVIALACRGLAPRLRFWGVVLGAGAAVGLRIIFAGIITTLMGLPYLKLVGGIALLVIATKLLVPEKQGEEAIPAAGRLWSAVRVVVVADFVMSLDNVIAIAAAANGSVPLLIIGLAVSVPLIVTGAALIMLLLTRLPFLIWAGSALLGWIAGEVIATDPAVAPWLHRFFDGGTGAFLDAVLGWVGIAPRFTAGGPGGEVLCGLLGVMIVLSAGSMIRNRRQVAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS96</accession>
    <name>Q3SS96_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ErfK/YbiS/YcfS/YnhG</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_1584</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YkuD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1584"/>
    <dbReference type="EnsemblBacteria" id="ABA04845">
        <property type="protein sequence ID" value="ABA04845"/>
        <property type="gene ID" value="Nwi_1584"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1584"/>
    <dbReference type="eggNOG" id="COG1376">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025126_0_0_5"/>
    <dbReference type="OMA" id="PMGVFSV"/>
    <dbReference type="OrthoDB" id="729553at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071972">
        <property type="term" value="F:peptidoglycan L,D-transpeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16913">
        <property type="entry name" value="YkuD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005490">
        <property type="entry name" value="LD_TPept_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038063">
        <property type="entry name" value="Transpep_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016915">
        <property type="entry name" value="UCP029342"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03734">
        <property type="entry name" value="YkuD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029342">
        <property type="entry name" value="UCP029342_ErfK/YbiS/YcfS/YnhG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141523">
        <property type="entry name" value="SSF141523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="YkuD" evidence="5">
        <location>
            <begin position="109"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="65"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="226"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="266"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="308"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="83"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="317"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA04845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="493" mass="52965" checksum="8B9368880C7F531D" modified="2005-10-11" version="1">MSKYWCFLRAIRWRSLSANFPLARAGAYPCPRRVGLGLVAAILIGLGSPAHSQFFWQEDYPEVFIEPPPQPPPQPPPVTRPQAHESKPRRLSKPEPTARVIRKPNGPIIIAVSIRRQTMKVYDGNGLFAETPVSTGMRGHSTPMGVFSVIQKRKWHRSNIYSNAPMPYMQRITWSGIAMHAGALPGYPASHGCIRMPLDFAVKMWGWTRIGARVIITPGEVTPADFSHPLLTARRPKSETVPAEVKASDAGNAMPQVEVSSIASDLPATGEPAAATPESAIGPVRTDSGNTQDADTTVTAAVKVDGDAAASPAGTPAAPEDQSQSSPESAEKTGLMAVSALKRNDRIAVFVSGKDSKIYVRQNFSPIFDAPITIAASDRPLGTHVFTARTTRDSKDDFRWSVVSLPAPAHRADRIHISKNQPARQTKVADATARPISVPENPTEALDRVSFPEDVMAKIASALSTGDSIVISDQGIAGSGETGEGTDFIVKLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSR5</accession>
    <name>Q3SSR5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Lipopolysaccharide biosynthesis</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_1415</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314685.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1415"/>
    <dbReference type="EnsemblBacteria" id="ABA04676">
        <property type="protein sequence ID" value="ABA04676"/>
        <property type="gene ID" value="Nwi_1415"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1415"/>
    <dbReference type="eggNOG" id="COG3206">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009912_3_0_5"/>
    <dbReference type="OMA" id="ACPTRLM"/>
    <dbReference type="OrthoDB" id="1184929at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009103">
        <property type="term" value="P:lipopolysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003856">
        <property type="entry name" value="LPS_length_determ_N_term"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02706">
        <property type="entry name" value="Wzz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Wzz" evidence="8">
        <location>
            <begin position="40"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="238"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="320"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA04676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="715" mass="78101" checksum="FC945F9DDFA9B768" modified="2005-10-11" version="1">MRFAFWRAGKKTMSDPETKSEQMVSPAPVSEVLPNPELGDIDLRSIGRVLLRKKRFIIIPTILAFALSLLIVNLITPRYKSEARILIDGRENVFLRPTGERNDERNVVDPEAVASQVQLVLSRDLAREVIRKNKLAENPEFDPVLNGVSPLKSLLALFGIGRDPLRMTPEERVLEAYYDRLTAYAVEKSRVIVIEFQSGDPELAASIANSIADGYLVLQLNARQEQAKAAGQWLSGEIDNLRKKVADAESRVEEFRSKSSLFIGINNTSLSNQQLGEINTQLNNARALKSDAESKARLIRGMLQTGKPIEASEVLNSELIRRLSEQRVTLRAQLAEQSSTLLDRHPRIRELKAQLADLDRQLREEAGKISRSLENDARIASGRVESLSNNLEQLKKLATSSNGDDVKLRALEREARAQRDLLESYLAKYREATTRENIDAAPSDGRIISRAIVSNTPAYPKKLPIVLIATLATLMLSTGTIVTGELLRITSPRSLAALQPAAPGMRGFRVDPAPAADLTDIAHLADDLRAAGKAAHKITVMGTGQNESIMLAALALARLLTDGARVVLVDLSPSSQALTATSVDPGAPGLADLMLGEVSFGQVITRDRLSPLHLVGTGRSDADRSLLQSPRLAIALDAFLRVYDHVLLVAGTATDLPAKILTEQARAIVVPNPSMNADARTRMSSQFKAIGFTTVTMLDPASIRPDMQIGPREAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSX2</accession>
    <name>Q3SSX2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ISBm1, transposase orfA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0365</name>
        <name evidence="2" type="ordered locus">Nwi_1358</name>
        <name evidence="3" type="ordered locus">Nwi_1718</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="2 4" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="2">Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0365"/>
    <dbReference type="EnsemblBacteria" id="ABA03632">
        <property type="protein sequence ID" value="ABA03632"/>
        <property type="gene ID" value="Nwi_0365"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04619">
        <property type="protein sequence ID" value="ABA04619"/>
        <property type="gene ID" value="Nwi_1358"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04979">
        <property type="protein sequence ID" value="ABA04979"/>
        <property type="gene ID" value="Nwi_1718"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0365"/>
    <dbReference type="KEGG" id="nwi:Nwi_1358"/>
    <dbReference type="KEGG" id="nwi:Nwi_1718"/>
    <dbReference type="eggNOG" id="COG3293">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055261_2_2_5"/>
    <dbReference type="OMA" id="RYGPWAT"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025161">
        <property type="entry name" value="Put_IS4/IS5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13340">
        <property type="entry name" value="DUF4096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="121" mass="13841" checksum="B798515B9E9B6447" modified="2005-10-11" version="1">MARFDLTDFEWSVIQPLLPNKSRGVARVDDRRVLNGIFWRLRTGAPWADIPARYGPHTTCVNRFNRWRKAGVWDRILSAISKAYDGDIQMIDSSSIRVHQHAANGQKNRRDPVAWVARAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST03</accession>
    <name>Q3ST03_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1327</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1327"/>
    <dbReference type="EnsemblBacteria" id="ABA04588">
        <property type="protein sequence ID" value="ABA04588"/>
        <property type="gene ID" value="Nwi_1327"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1327"/>
    <dbReference type="eggNOG" id="COG3904">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_972064_0_0_5"/>
    <dbReference type="OMA" id="VACEPNC"/>
    <dbReference type="OrthoDB" id="1230850at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229214" evidence="2">
        <location>
            <begin position="31"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="305" mass="32431" checksum="CD22C3EF9B464C4B" modified="2005-10-11" version="1">MRFAQSSGFCGRALLYAAAFGLAFSGPSAALEKVGPAKVNVEARKQPMRFSWVACEPRCGSWITAVGLVTSETLKTFNDFAAGRDLAGATVVLDSGGGSVNDAIALGRRWRELGLRTTVGTSVVVTTAAGDRAAVLPEAYCESMCVFVLLSGKTRYVPERAHVRVHQIWMGDRAENARAASYTAEDLTTVERDIGRLAKYTFEMGGSGDLLYLALNIPPWEALYELSRSELRSTNLVTTDRVADVLPDGEVAEAPVADLVTKPVQDRFPGATNAPAVVTMTGQPTKTAEAEPSGAAVVTTVPARQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-17" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST04</accession>
    <name>YIDD_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative membrane protein insertion efficiency factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">Nwi_1326</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Could be involved in insertion of integral membrane proteins into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0161 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314605.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ST04"/>
    <dbReference type="STRING" id="323098.Nwi_1326"/>
    <dbReference type="EnsemblBacteria" id="ABA04587">
        <property type="protein sequence ID" value="ABA04587"/>
        <property type="gene ID" value="Nwi_1326"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1326"/>
    <dbReference type="eggNOG" id="COG0759">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144811_0_0_5"/>
    <dbReference type="OMA" id="ARWYLPW"/>
    <dbReference type="OrthoDB" id="1912220at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00386">
        <property type="entry name" value="UPF0161_YidD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002696">
        <property type="entry name" value="Membr_insert_effic_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33383">
        <property type="entry name" value="PTHR33383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01809">
        <property type="entry name" value="YidD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01234">
        <property type="entry name" value="Haemolytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00278">
        <property type="entry name" value="TIGR00278"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000253131" description="Putative membrane protein insertion efficiency factor">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00386"/>
        </source>
    </evidence>
    <sequence length="117" mass="13158" checksum="E487C15A085D3940" modified="2005-10-11" version="1">MKTIPSEVRCSRLCPACTNAALRLPRNAGRALIWIYRHTLSPLVGFNCRHLPTCSAYGDEAIARFGLWGGGWMTLARILRCRPWGTSGIDNVPVAKPSGATWYRPWRYGRWRGVNAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST21</accession>
    <name>Q3ST21_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, BolA protein family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1309</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BolA/IbaG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314590.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1309"/>
    <dbReference type="EnsemblBacteria" id="ABA04570">
        <property type="protein sequence ID" value="ABA04570"/>
        <property type="gene ID" value="Nwi_1309"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1309"/>
    <dbReference type="eggNOG" id="COG0271">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109462_4_2_5"/>
    <dbReference type="OMA" id="HQLVYGA"/>
    <dbReference type="OrthoDB" id="2009812at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002634">
        <property type="entry name" value="BolA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036065">
        <property type="entry name" value="BolA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01722">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003113">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82657">
        <property type="entry name" value="SSF82657"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="77" mass="8382" checksum="08155C3D81482212" modified="2005-10-11" version="1">MAMEARDIESMIKEAIPDARVTIQDLAGDGNHYSATVISESFRGKSRVQQHQLVYQSLKGQMGGVLHALALQTGVPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST40</accession>
    <name>Q3ST40_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase, IS4 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0215</name>
        <name evidence="2" type="ordered locus">Nwi_1290</name>
        <name evidence="3" type="ordered locus">Nwi_2924</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="2 4" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="2">Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03483">
        <property type="protein sequence ID" value="ABA03483"/>
        <property type="gene ID" value="Nwi_0215"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04551">
        <property type="protein sequence ID" value="ABA04551"/>
        <property type="gene ID" value="Nwi_1290"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06174">
        <property type="protein sequence ID" value="ABA06174"/>
        <property type="gene ID" value="Nwi_2924"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0215"/>
    <dbReference type="KEGG" id="nwi:Nwi_1290"/>
    <dbReference type="KEGG" id="nwi:Nwi_2924"/>
    <dbReference type="HOGENOM" id="CLU_055261_9_3_5"/>
    <dbReference type="OMA" id="HLAYAYI"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="75" mass="8826" checksum="68D3A36DB9638868" modified="2005-10-11" version="1">MRALIAGMGAEAVIPSNRTRKIIIPHDAGLYKHRNRIERCFNRLKHFRRFATRYDRRTVHFTGFVHLAAALIWLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST66</accession>
    <name>Q3ST66_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1264</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314553.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1264"/>
    <dbReference type="EnsemblBacteria" id="ABA04525">
        <property type="protein sequence ID" value="ABA04525"/>
        <property type="gene ID" value="Nwi_1264"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1264"/>
    <dbReference type="HOGENOM" id="CLU_1775503_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="146" mass="16973" checksum="47C767D197DF1650" modified="2005-10-11" version="1">MKKTRHIKLMERPLLIQPEAKVLQGFLSAAVRIQIKVSGLRKLPRGVSDPDRQLPRLIQISHLFNGLLQSRKLKTQADHQLLAAAECYPELLRRQPHSPVWAPSYRFCYELSRWLPFLNTYRTMCIAPEPGFKRMLEDICELRLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST94</accession>
    <name>Q3ST94_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CDP-diacylglycerol--serine O-phosphatidyltransferase</fullName>
            <ecNumber evidence="4">2.7.8.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Phosphatidylserine synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_1235</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a CDP-1,2-diacyl-sn-glycerol + L-serine = a 1,2-diacyl-sn-glycero-3-phospho-L-serine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16913"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.8" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314526.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1235"/>
    <dbReference type="EnsemblBacteria" id="ABA04497">
        <property type="protein sequence ID" value="ABA04497"/>
        <property type="gene ID" value="Nwi_1235"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1235"/>
    <dbReference type="eggNOG" id="COG1183">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049944_1_0_5"/>
    <dbReference type="OMA" id="IFFDFFD"/>
    <dbReference type="OrthoDB" id="1232102at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003882">
        <property type="term" value="F:CDP-diacylglycerol-serine O-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004533">
        <property type="entry name" value="CDP-diaglyc--ser_O-PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012616">
        <property type="entry name" value="CDP-OH_P_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08009">
        <property type="entry name" value="CDP-OH_P_tran_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00473">
        <property type="entry name" value="pssA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="101"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="130"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="199"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="CDP-OH_P_tran_2" evidence="11">
        <location>
            <begin position="198"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="256"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA04497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="292" mass="31607" checksum="89AC74C9AFA406AF" modified="2005-10-11" version="1">MRRRRFRPIPVRMLVPNFITLLAICAGLTAIRLSTEGRMELAVGAIVFAAVLDGLDGRVARMIKGQSKFGAELDSLADFVNFGVAPGLILYFWQLHDLSNVGWIAAMIFAISGGLRLARFNATMNDENKPAFAANFFTGVPAPAGAIAVLLPIYLSFLGIPQPPAVLTAFYTLLIAILMVSRLPVFSGKTVGMRVQPELVLPVFVSVVFVIALLISYPWVVLSFGSIIFLLSLPVGWKSYHDQELKAAVQETFPDAAASAEAAPPASAATKPAPPTIPIRQRKQDNRPSRPH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STD7</accession>
    <name>CCME_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cytochrome c-type biogenesis protein CcmE</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cytochrome c maturation protein E</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Heme chaperone CcmE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ccmE</name>
        <name evidence="1" type="synonym">cycJ</name>
        <name type="ordered locus">Nwi_1192</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Heme chaperone required for the biogenesis of c-type cytochromes. Transiently binds heme delivered by CcmC and transfers the heme to apo-cytochromes in a process facilitated by CcmF and CcmH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
            <orientation evidence="1">Periplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CcmE/CycJ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314483.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3STD7"/>
    <dbReference type="SMR" id="Q3STD7"/>
    <dbReference type="STRING" id="323098.Nwi_1192"/>
    <dbReference type="EnsemblBacteria" id="ABA04454">
        <property type="protein sequence ID" value="ABA04454"/>
        <property type="gene ID" value="Nwi_1192"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1192"/>
    <dbReference type="eggNOG" id="COG2332">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079503_1_1_5"/>
    <dbReference type="OMA" id="TFRVGGM"/>
    <dbReference type="OrthoDB" id="1724595at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017003">
        <property type="term" value="P:protein-heme linkage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01959">
        <property type="entry name" value="CcmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004329">
        <property type="entry name" value="CcmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036127">
        <property type="entry name" value="CcmE-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34128">
        <property type="entry name" value="PTHR34128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03100">
        <property type="entry name" value="CcmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82093">
        <property type="entry name" value="SSF82093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword id="KW-0349">Heme</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0735">Signal-anchor</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000238824" description="Cytochrome c-type biogenesis protein CcmE">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="1"/>
            <end position="7"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Signal-anchor for type II membrane protein" evidence="1">
        <location>
            <begin position="8"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="topological domain" description="Periplasmic" evidence="1">
        <location>
            <begin position="29"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="binding site" description="covalent" evidence="1">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="1">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="162" mass="17358" checksum="9DE2906827CD94D0" modified="2005-10-11" version="1">MTRKQRRLTMIGGALVVLGIAAALVLNALRDSIVFFSTPMMVSEKHIQPGQRFRLGGLVQTGSLVRGDNLAVSFRVSDGSATLPVSYKGILPDLFREGQGVVAEGALDNSGVFQADTVLAKHDETYMPKEVADALKKQGHWKDDYGAQPGAAEASSKQEVSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STL4</accession>
    <name>Q3STL4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Heat shock protein Hsp20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1115</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314408.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1115"/>
    <dbReference type="EnsemblBacteria" id="ABA04377">
        <property type="protein sequence ID" value="ABA04377"/>
        <property type="gene ID" value="Nwi_1115"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1115"/>
    <dbReference type="eggNOG" id="COG0071">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046737_12_0_5"/>
    <dbReference type="OMA" id="DLRTYAQ"/>
    <dbReference type="OrthoDB" id="1523369at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="SSF49764"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="51"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="162" mass="18297" checksum="49A40FC95CF7964D" modified="2005-10-11" version="1">MAIRDLIPWSRNQEVTSARASYDPFMTLHREMNRLFDDVFRSFEPGQTPLMEGRFGWPRIELGETDKAVTVSAELPGMTEKDVQVEIANGTLTIRGEKKNERANGGKYVTERYYGSFQRQIPLDDVDEDKAEASFRNGVLTISLPKSANPRAGVKRIAISTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STN7</accession>
    <name>Q3STN7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1092</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04354">
        <property type="protein sequence ID" value="ABA04354"/>
        <property type="gene ID" value="Nwi_1092"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1092"/>
    <dbReference type="eggNOG" id="ENOG5030VJ3">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1785391_0_0_5"/>
    <dbReference type="OMA" id="YFPRHLI"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="145" mass="15214" checksum="407D52F408C1BE89" modified="2005-10-11" version="1">MMAETLYFPRHLIIGGAMVSGILLALAVHMLSAQMGLDLGGLWRSDAGEFMPASAAIAWWLVATASFSGSYFVATLMHSAASNRIQQRLRQFLIAIAVLFLAGAGQAASAPSTAPTLANFITGLVVLGLGATMAFCGAYFTLRKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STP7</accession>
    <name>Q3STP7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phosphomethylpyrimidine kinase / hydroxymethylpyrimidine kinase</fullName>
            <ecNumber evidence="5">2.7.4.7</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_1082</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + ATP = 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + ADP</text>
            <dbReference type="Rhea" id="RHEA:19893"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:58354"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.7"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4-amino-5-hydroxymethyl-2-methylpyrimidine + ATP = 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23096"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16892"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58354"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.49"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5-amino-1-(5-phospho-D-ribosyl)imidazole: step 3/3.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.7" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1082"/>
    <dbReference type="EnsemblBacteria" id="ABA04344">
        <property type="protein sequence ID" value="ABA04344"/>
        <property type="gene ID" value="Nwi_1082"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1082"/>
    <dbReference type="eggNOG" id="COG0351">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020520_0_1_5"/>
    <dbReference type="OMA" id="DNRHTHG"/>
    <dbReference type="UniPathway" id="UPA00060">
        <property type="reaction ID" value="UER00138"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008902">
        <property type="term" value="F:hydroxymethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008972">
        <property type="term" value="F:phosphomethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01169">
        <property type="entry name" value="HMPP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045029">
        <property type="entry name" value="HMP/HMP-P_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004399">
        <property type="entry name" value="HMP/HMP-P_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858">
        <property type="entry name" value="PTHR20858"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00097">
        <property type="entry name" value="HMP-P_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phos_pyr_kin" evidence="4">
        <location>
            <begin position="14"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="271" mass="27823" checksum="FA4B9C4AE59D590D" modified="2005-10-11" version="1">MMTVPVALTIAGSDSSGGAGIQADLKTFAALGVYGASVITALTAQNTQGVAAIHHVPAHFVTAQIDAVFGDLDVGALKIGMVAQVAVIDAIAAGLSRWAPAHVVLDPVMVATSGAHLLESDAIEALRSMLVPRAQLLTPNLPEAAVLLDEPVAGGETDIVRQGQRLLELGCAAVLIKGGHMHGPESIDYLVTRQGAMTFAAPRAVTRNTHGTGCSLSSAIAAGLAKGDGMETAVRGAKAYVTAAIAAGDRLNIGRGHGPIHHFHQFEHFRF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STQ8</accession>
    <name>HLDE_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Bifunctional protein HldE</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="1">D-beta-D-heptose 7-phosphate kinase</fullName>
                <ecNumber evidence="1">2.7.1.167</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="1">D-beta-D-heptose 7-phosphotransferase</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="1">D-glycero-beta-D-manno-heptose-7-phosphate kinase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="1">D-beta-D-heptose 1-phosphate adenylyltransferase</fullName>
                <ecNumber evidence="1">2.7.7.70</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="1">D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="1" type="primary">hldE</name>
        <name type="ordered locus">Nwi_1071</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the phosphorylation of D-glycero-D-manno-heptose 7-phosphate at the C-1 position to selectively form D-glycero-beta-D-manno-heptose-1,7-bisphosphate.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Catalyzes the ADP transfer from ATP to D-glycero-beta-D-manno-heptose 1-phosphate, yielding ADP-D-glycero-beta-D-manno-heptose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + D-glycero-beta-D-manno-heptose 7-phosphate = ADP + D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27473"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:60204"/>
            <dbReference type="ChEBI" id="CHEBI:60208"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.167"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + D-glycero-beta-D-manno-heptose 1-phosphate + H(+) = ADP-D-glycero-beta-D-manno-heptose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:27465"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59967"/>
            <dbReference type="ChEBI" id="CHEBI:61593"/>
            <dbReference type="EC" id="2.7.7.70"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; ADP-L-glycero-beta-D-manno-heptose biosynthesis; ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; ADP-L-glycero-beta-D-manno-heptose biosynthesis; ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the cytidylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.167" evidence="1"/>
    <dbReference type="EC" id="2.7.7.70" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314367.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3STQ8"/>
    <dbReference type="SMR" id="Q3STQ8"/>
    <dbReference type="STRING" id="323098.Nwi_1071"/>
    <dbReference type="EnsemblBacteria" id="ABA04333">
        <property type="protein sequence ID" value="ABA04333"/>
        <property type="gene ID" value="Nwi_1071"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1071"/>
    <dbReference type="eggNOG" id="COG0615">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2870">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021150_2_1_5"/>
    <dbReference type="OMA" id="CEFANAA"/>
    <dbReference type="OrthoDB" id="1030724at2"/>
    <dbReference type="UniPathway" id="UPA00356">
        <property type="reaction ID" value="UER00437"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00356">
        <property type="reaction ID" value="UER00439"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033785">
        <property type="term" value="F:heptose 7-phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033786">
        <property type="term" value="F:heptose-1-phosphate adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097171">
        <property type="term" value="P:ADP-L-glycero-beta-D-manno-heptose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01172">
        <property type="entry name" value="RfaE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01603">
        <property type="entry name" value="HldE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023030">
        <property type="entry name" value="Bifunc_HldE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011913">
        <property type="entry name" value="RfaE_dom_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011914">
        <property type="entry name" value="RfaE_dom_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02198">
        <property type="entry name" value="rfaE_dom_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02199">
        <property type="entry name" value="rfaE_dom_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00583">
        <property type="entry name" value="PFKB_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000255769" description="Bifunctional protein HldE">
        <location>
            <begin position="1"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Ribokinase">
        <location>
            <begin position="1"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Cytidylyltransferase">
        <location>
            <begin position="358"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="275"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="205"/>
            <end position="208"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01603"/>
        </source>
    </evidence>
    <sequence length="488" mass="51679" checksum="19A73CCA9BBC9F24" modified="2005-10-11" version="1">MIDFDGLSNAFSGQTVLCVGDLMLDEFVYGEISRISPEAPAPVIAVQRSETNVGGAGNVARNIAALGARCIFVGLAGEDDAGARLRDTLSRERLIEPLLISDPARSTTRKVRFVSEHFSTHMLRADWETLAPASGEVEQALIDAILPQLPCADIVLLSDYAKGVLTTRVIRSVIDGARRLGKRVIVDPKSADFGIYRGATLLTPNSKEFTDATGRRADDQASLAKAARDLMRIAECEALLVTQSERGMTLVPGEGEVIHVPAHTVKVRDVTGAGDTVVATLAVSLAAGADWEAALRTASAAAAVAVAKSGTAIVTLAELRRNILPPASLAAEEKIARSAGDLDQHLTAWREQGLRIGFTNGCFDILHPGHVKVLTGARAACDRLIVGLNSDASVKRLKGEGRPIQNERARAEVLAALEAVDLVAIFEEDTPLNLIGRIQPNVLVKGGDYSLEQVVGQELVTARGGEVILIDILQGFSTTSLVKRAGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STV7</accession>
    <name>Q3STV7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1022</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04284">
        <property type="protein sequence ID" value="ABA04284"/>
        <property type="gene ID" value="Nwi_1022"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1022"/>
    <dbReference type="HOGENOM" id="CLU_2247189_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="104" mass="11296" checksum="5C8B3783C8385FBB" modified="2005-10-11" version="1">MRARKHAINNGRASDAPALIGKLQAKTQMVAGQGPAMAEQDISSASKPFQRFQANHILGLDQLGLTKGMGYPGSREERVKPHHGVPLLIHSEAGRLQPRHTMPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STV9</accession>
    <name>Q3STV9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1020</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04282">
        <property type="protein sequence ID" value="ABA04282"/>
        <property type="gene ID" value="Nwi_1020"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1020"/>
    <dbReference type="eggNOG" id="ENOG50345WZ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2341221_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="83" mass="8976" checksum="D603D3893327F6CD" modified="2005-10-11" version="1">MDAQVLPEDHPAARKLSELFGDHTFFVDESGLKVLESTEPAAAEAQRGSVVSLADWNDGKFSSLRPHDPAVTGTVIVLEQSKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STZ7</accession>
    <name>Q3STZ7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Response regulator receiver modulated diguanylate phosphodiesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0982</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314283.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0982"/>
    <dbReference type="EnsemblBacteria" id="ABA04244">
        <property type="protein sequence ID" value="ABA04244"/>
        <property type="gene ID" value="Nwi_0982"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0982"/>
    <dbReference type="eggNOG" id="COG2200">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_70_2_5"/>
    <dbReference type="OMA" id="WTEYQAI"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="24"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="EAL" evidence="3">
        <location>
            <begin position="147"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="404" mass="44337" checksum="C1F388040C435EF3" modified="2005-10-11" version="1">MTDTARQDDRIAETPFGKRRVKPRACIVESKHHVRSMLSEALEEQGFIPLACATLEDLDASLRSDFPDIIVISFSMNGLSVPGILNLLATRICAAKVLLIGPPDAPAVKAARQLGENLALDLLPVLSTPFSDRDLRARVAALVPAEPPSPPVDVAEALGAGWLELWYQPKINARSLSLSGAEALIRMRHPNWGIVPPARFIPDEGDPHFRALSDFVIGRVFQDWRYLVAKSGSIHISINLPLAFFTDTNAVDDLRMQFPNHPAFDGLTVEVDGSEVVQNLSLAVEAAHRLRFHNIGLSIDDVGTEWPTLAALKDFPFAEIKLDRKFVDGSSSARLKRSVCRQIVDLAKSHGVKTVAEGVETRDDFLAMHGIGVDFIQGFLFGKPMTTRKFAHSSRQRPTFAADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU18</accession>
    <name>Q3SU18_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0961</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314264.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0961"/>
    <dbReference type="EnsemblBacteria" id="ABA04223">
        <property type="protein sequence ID" value="ABA04223"/>
        <property type="gene ID" value="Nwi_0961"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0961"/>
    <dbReference type="eggNOG" id="ENOG502ZA9C">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_429508_0_0_5"/>
    <dbReference type="OMA" id="WNFASIH"/>
    <dbReference type="OrthoDB" id="1554254at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="612"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="612"/>
            <end position="628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="640" mass="68096" checksum="B1A71B3B700FF690" modified="2005-10-11" version="1">MISGGITEIIWHFAGYLRIFDDIARFRIAHDEGAYRSHTEDYTTNRPQFAFTPIDDEMSSRPVAIQNPSVMGGAGHHAPTSHLSPVPDVGPDKINPIHASGFLPPMSPGAGGGVGGSDVIKVVYQEEGAQSEVQTRQFNHMYDNDALVGTQDSNLLIALRAESDHAIATMIDGADSAIPVDWRVPSNTADLPAFVVKHHQALADRDGAPDPHSVQQGYYVNGVLQDPALRPPDQLSASDPAPVPDFGSGPGQWALDGGNTATNGAYIVDLTGSARTMLVVGDYYKTNAIVQTNSYMDNDHVTVGGSGAGITTGGNQATNVADFVQNPGVYESMPSSFAGPNWSVDVVKGDYFNIRILLQSNYLSDNDVTMQSSSSTHYEVHAGQNELENFVLINDGNFNYDVIVVGGSYHGMNVIFQNNILYNNDMVMMTGDGVEPGQTVSTGGNQLTNTATIESYGGADTQPWNSDLDSLIHNISGGATELDPSFGELIAGNGGTLNILYITGDYYDVNAIWQTNIVADANMAIQLQGSPSEVTAGYYGDSMTTQNVATGGNVLSNDAVIVDVGSTNIHVNGQVYGDSILIQANLVEEDSDQVTQTDPQALVPEVIAFIGPNDTNDNSDTQPHATTSIPHDDPMANILH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU22</accession>
    <name>Q3SU22_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulatory protein, LuxR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_0957</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314260.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0957"/>
    <dbReference type="EnsemblBacteria" id="ABA04219">
        <property type="protein sequence ID" value="ABA04219"/>
        <property type="gene ID" value="Nwi_0957"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0957"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_8_5"/>
    <dbReference type="OMA" id="HIRHIMR"/>
    <dbReference type="OrthoDB" id="1687028at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="130"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="4">
        <location>
            <begin position="268"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA04219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="342" mass="37599" checksum="A83B286C3D28C5A5" modified="2005-10-11" version="1">MSANEYRCSDGSHFSHHFTPTDQRVRKTSAPVRVAYPRPPLDPRPQLNVGSASCGASPPTRRHRQARLQNAAIVNVDSPWQARPKNPAARPSSPPPSTQAADLDHNSRTAQCVSGTKDSFDAGPKPAQNSIIIIDERALVRDCLVYCLQARYVSHAVFSFATLSDWMKVAGDFPAPSVILLYARTPQKKTLNDIDDIESLARVAVSAPLIIVSDVEDGSRVMRAIESGARGYIPTSMTLGIALEAVRLVEAGGTFVPISVMSSFQNAGHDESQLFTSRQIMVIEALRRGKANKQIAYELGMCESTVKAHIRHIMRKLKARNRTEVAMMVSSLFERLEHDRAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU44</accession>
    <name>Q3SU44_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="12">MscS Mechanosensitive ion channel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_0935</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314238.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0935"/>
    <dbReference type="EnsemblBacteria" id="ABA04197">
        <property type="protein sequence ID" value="ABA04197"/>
        <property type="gene ID" value="Nwi_0935"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0935"/>
    <dbReference type="eggNOG" id="COG3264">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011796_1_0_5"/>
    <dbReference type="OMA" id="RATEIQM"/>
    <dbReference type="OrthoDB" id="1903165at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022249">
        <property type="entry name" value="DUF3772"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011066">
        <property type="entry name" value="MscS_channel_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011014">
        <property type="entry name" value="MscS_channel_TM-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12607">
        <property type="entry name" value="DUF3772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="SSF50182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82689">
        <property type="entry name" value="SSF82689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82861">
        <property type="entry name" value="SSF82861"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0346">Stress response</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229251" evidence="10">
        <location>
            <begin position="22"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="232"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="279"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="314"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="356"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="388"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="431"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="466"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="511"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="555"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="603"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="632"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3772" evidence="11">
        <location>
            <begin position="160"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="29"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="41"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA04197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="845" mass="90193" checksum="EE3BB3226C71445C" modified="2005-10-11" version="1">MKTIRAAIFFIIFLSWLPLNAASAATATAPAAGNGKPASPAAAPAAPPAPAEDLPSVPAPEFPRAQSKLDEWKQTLDQVEAMLRARVIHDTVLNDLRDRSATAQREARELVSRITPRLEAASARAAELAPSPEATAPQPDDVKLERAKLLAEIAARQGVIQQAKLINVRAQQTIDAISDRRRAVFNESVLQRSASLVNPTFWLTAANAAPIAAGRLVDLLAKWGRVLSSQPLRAASGFVIAAIVVLGFFLSPGRRWWSRWTTRDPEIANPSALRKTGSAAAIVFTDTALTAVALLVLYQALLTLEVLPGDVTPIVRALFLGITFGFFFIRLTSAVLAPGRPSWRLIELDNAAADGLISYSVLLAMVTAVGIVLDALSLAIAPPIELTIASQATLAITKALLFMGALRVAAATEAEEESAAPSSAQRSAWRLLIPVGWVMAMAAVIGPLAGYVALGRFITIEMVVMVVALMSFILLSRFSAVLVNTTFAFNGAIGRFLRQTAGLGSGAVRQIAAVLGGLIQLSLIGLTAFFILTTWGIRSDDVVTSMSSAFFSFQIGNITISPSQILGALAVLTIGVVATRALKRWLEERFLPETNLDVGVRSSIGTGAGYAGVILATLIALSYVGLNLQNVAIVAGALSVGIGFGLQSIINNFVSGLILLVERPIKVGDRIEVGTRMGVVKRINVRATEIATYDNVSVIVPNAELISGQVVNWMHGSYSARLSVAVSTSSNADPDKVLAILLDIVSRHPRALKSPEPFAILNNFGPDALEFSVFFHVAHIGIDAGAANEVRLEILKRFRKEGIELPNAKRDLHVHNLDRIETLVRDLADSERSGASRRRIRSIES</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU48</accession>
    <name>Q3SU48_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sugar isomerase, KpsF/GutQ family protein</fullName>
            <ecNumber evidence="8">5.3.1.13</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_0931</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the SIS family. GutQ/KpsF subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.13" evidence="8"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0931"/>
    <dbReference type="EnsemblBacteria" id="ABA04193">
        <property type="protein sequence ID" value="ABA04193"/>
        <property type="gene ID" value="Nwi_0931"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0931"/>
    <dbReference type="eggNOG" id="COG0517">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0794">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040681_13_1_5"/>
    <dbReference type="OMA" id="LMACLMR"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019146">
        <property type="term" value="F:arabinose-5-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05014">
        <property type="entry name" value="SIS_Kpsf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004800">
        <property type="entry name" value="KdsD/KpsF-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035474">
        <property type="entry name" value="SIS_Kpsf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004692">
        <property type="entry name" value="KdsD_KpsF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00393">
        <property type="entry name" value="kpsF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0129">CBS domain</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SIS" evidence="7">
        <location>
            <begin position="41"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="6">
        <location>
            <begin position="209"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="6">
        <location>
            <begin position="274"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="site" description="Catalytically relevant" evidence="4">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <feature type="site" description="Catalytically relevant" evidence="4">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <feature type="site" description="Catalytically relevant" evidence="4">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <feature type="site" description="Catalytically relevant" evidence="4">
        <location>
            <position position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR004692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR004692-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR004692-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA04193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="325" mass="34015" checksum="B2A3040FEA8F6121" modified="2005-10-11" version="1">MIDQANIAIESALRTFEAEAGGVSALAAALKSDLGLAFAAATDLIRNAKGRLIVTGLGKSGHIGRKIAATFASTGTPAFFVHAAEASHGDLGMITADDVIMALSWSGEQPEMKNLITYAKRFRIPLIAMTAERDSTLGKAADVALVQPKAREACPHNLAPTTSSLMQLALGDGLAIALLEGRGFTSVDFSVLHPGGKLGALLKYTRDLMHSGDAIPLKPLGTKMSEALVEMTSKGFGCVGVTDGQGNLVGIVTDGDLRRHMRPDLMTARVDDVMTPHPKTIGRDLLAGEALEILNSSKITALIVTEGKKPVGIVHLHDLLRAGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUC8</accession>
    <name>Q3SUC8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conjugal transfer protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0849</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314160.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0849"/>
    <dbReference type="MEROPS" id="S26.014"/>
    <dbReference type="EnsemblBacteria" id="ABA04113">
        <property type="protein sequence ID" value="ABA04113"/>
        <property type="gene ID" value="Nwi_0849"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0849"/>
    <dbReference type="eggNOG" id="COG4959">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104604_0_0_5"/>
    <dbReference type="OMA" id="VWQGCHV"/>
    <dbReference type="OrthoDB" id="1741894at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10502">
        <property type="entry name" value="Peptidase_S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="SSF51306"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_S26" evidence="1">
        <location>
            <begin position="8"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="186" mass="20399" checksum="16F8F82DAC527F6D" modified="2005-10-11" version="1">MTARRRTLLAMLTGAALIVAPAWSRPELRFIWNASASVPIGLYRIVPGNHVDVTDLAVVMPPAELAAFLDERGYLPRGLPLIKRVLALGGTRICRRDSAIIAYDGIYGQALARDTRGRPLPDWQGCRTLHDGEAFFMNWDSPDSFDSRYFGPLPISTIVGRAIPIWTTDGTDPAAESLRAPVSDEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUG5</accession>
    <name>Q3SUG5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0811</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314124.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0811"/>
    <dbReference type="EnsemblBacteria" id="ABA04076">
        <property type="protein sequence ID" value="ABA04076"/>
        <property type="gene ID" value="Nwi_0811"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0811"/>
    <dbReference type="eggNOG" id="ENOG502Z8CS">
        <property type="taxonomic scope" value="Bacteria"/>
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    <dbReference type="HOGENOM" id="CLU_117594_0_0_5"/>
    <dbReference type="OMA" id="AQWAQNT"/>
    <dbReference type="OrthoDB" id="1627752at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="190" mass="20882" checksum="98683A5BCBDC3895" modified="2005-10-11" version="1">MGLSRRAYARHRGVSDMAVRKAIASGRIAVEDDGTIDPEKADRAWGSSSDPAQVRPVAKSPPPPRGTPRPVPIAAVEAVRETLRESGEPAPAAGNMTFVQARTANEVIKAQERRIRLGKLKGDLVDRSRAVSTVFALARRERDAWVQWPARAAALIAAELQIDPHRCEQVLEAHVRRHLEELSQIGIELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUH7</accession>
    <name>Q3SUH7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0799</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314112.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0799"/>
    <dbReference type="EnsemblBacteria" id="ABA04064">
        <property type="protein sequence ID" value="ABA04064"/>
        <property type="gene ID" value="Nwi_0799"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0799"/>
    <dbReference type="eggNOG" id="ENOG502ZBQE">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119921_0_0_5"/>
    <dbReference type="OMA" id="VEPFWID"/>
    <dbReference type="OrthoDB" id="1570325at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="118" mass="12566" checksum="29BE7D26DADA67EC" modified="2005-10-11" version="1">MLKLEPVSVEPFWIDVLPGVRIQFRPVSVAAMLIARGAAGEALKAGGEQATIEAGAAFTRSLAQTGIIAWEGIGNAKGKPVDPDKAAIDQLLELWPAFDAIDRLYVGPALTRLDEKNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUJ8</accession>
    <name>Q3SUJ8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Respiratory nitrate reductase gamma subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">Nwi_0778</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314092.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0778"/>
    <dbReference type="EnsemblBacteria" id="ABA04043">
        <property type="protein sequence ID" value="ABA04043"/>
        <property type="gene ID" value="Nwi_0778"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0778"/>
    <dbReference type="eggNOG" id="COG2181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092378_0_0_5"/>
    <dbReference type="OMA" id="MVSGGFF"/>
    <dbReference type="OrthoDB" id="1873056at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009325">
        <property type="term" value="C:nitrate reductase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008940">
        <property type="term" value="F:nitrate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042128">
        <property type="term" value="P:nitrate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023234">
        <property type="entry name" value="NarG-like_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036197">
        <property type="entry name" value="NarG-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003816">
        <property type="entry name" value="Nitrate_red_gam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02665">
        <property type="entry name" value="Nitrate_red_gam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103501">
        <property type="entry name" value="SSF103501"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00351">
        <property type="entry name" value="narI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0349">Heme</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0534">Nitrate assimilation</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="46"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="82"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="187"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Nitrate_red_gam" evidence="14">
        <location>
            <begin position="4"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABA04043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="225" mass="25597" checksum="9F50A38479733BC0" modified="2005-10-11" version="1">MYDVFLFAVLPYLAIVVAVLGGVHRYRTNRFSYSTLSSQLLENRRLFWGSVPWHYGIVVVLLAHMIGFLVPGMWSALTSTPTALYTVELIGFAFGFAALIGLCVLIVRRFTTERILAVTSPMDGILLVALLVQVALGFWVSLAYRWGADWYIDTAVPWLISLVSLNPQTHYVSALPFPVKAHMLTGFLVIALFPFSRLVHLIMFPFWYLWRPYQIQIRNTVRSAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUK5</accession>
    <name>Q3SUK5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">4-amino-4-deoxy-L-arabinose transferase and related glycosyltransferases of PMT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_0771</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314085.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0771"/>
    <dbReference type="EnsemblBacteria" id="ABA04036">
        <property type="protein sequence ID" value="ABA04036"/>
        <property type="gene ID" value="Nwi_0771"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0771"/>
    <dbReference type="eggNOG" id="COG1807">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_582205_0_0_5"/>
    <dbReference type="OMA" id="APKFINA"/>
    <dbReference type="OrthoDB" id="1255620at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038731">
        <property type="entry name" value="RgtA/B/C-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13231">
        <property type="entry name" value="PMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="118"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="168"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="293"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="348"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="374"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="PMT_2" evidence="9">
        <location>
            <begin position="93"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA04036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="503" mass="55290" checksum="FEEF0A6B223595E7" modified="2005-10-11" version="1">MRLDKASGRSAAGQLWDKVFVPGAWLLAKFSDLDRSRWPILWVIGLFWIQAIPATIIRASNLEEGRIIAMARGAVEDGHWLTPFVYGERFAERPVLLSWLAAFFGEATGSVTLWSLRIPHLCFFLAGALLIYSLLRSMTGKSAAIFGALCWISMPVVAPKFINAEPDIVLSVLLFSAFCIWWHGTRDKGITPGRWLCISVLIALAGLTKGPQEVAYFTLGVGAYILLRQRDQIPGFIAAHIGAGLVIGGWYGLVYQTGDADTWKAHSRLTQTTDVLATVLGHLDFVKSLAVEFLPGSILIGPAIMIAIDRWRDGRRDMRDDLLLAAILYSVACTLVLLFWPGKVAARYAMPGTMTLAVICGLLFENWRHSHPRVIAPALVVTCMIFGGLLVRGWIVMPVVPHLFQESRLAGQAIDAILKGDSKPLYVAGGDSTDFNMLVYVRGPISTIALDELAELNVPAIAVLRPEHQRAVLQTRPELHLIEKDSIVSGKTHYNIIKIIPPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUN9</accession>
    <name>Q3SUN9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0736</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04002">
        <property type="protein sequence ID" value="ABA04002"/>
        <property type="gene ID" value="Nwi_0736"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0736"/>
    <dbReference type="HOGENOM" id="CLU_965877_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="288" mass="33163" checksum="A4D4AD1C71C1991F" modified="2005-10-11" version="1">MMKVGRKVIVAHACLLSAVTGRAEAYRTKRELVKEYIPLRSCRSHQRSWKLCELPGFDLSCGKPILALARVSNLSARKGRGRALSGSRQVPGATPKNRRYRNAKISEHRLKRVVECFARDLTAAETVKATKLSAPTVNLIFHRLRERMRDHGLFIPQFGDEPHPLKEVFNPKHRGVPEHLHDLHAIERIHRILCAQHLKGFERLPASEPKNVEKAIRLLRFNGMQPKRRYQIWEALKQAEVDTGASTTRPFDPRHIHKDSDILINELHINPQDAFFAYLWALLLRQPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUQ3</accession>
    <name>Q3SUQ3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0722</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314042.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0722"/>
    <dbReference type="EnsemblBacteria" id="ABA03988">
        <property type="protein sequence ID" value="ABA03988"/>
        <property type="gene ID" value="Nwi_0722"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0722"/>
    <dbReference type="eggNOG" id="ENOG502Z7SC">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054566_0_0_5"/>
    <dbReference type="OMA" id="FWGSFDL"/>
    <dbReference type="OrthoDB" id="853902at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046038">
        <property type="entry name" value="DUF5996"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19459">
        <property type="entry name" value="DUF5996"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="322" mass="35586" checksum="834E506C7F1F8B76" modified="2005-10-11" version="1">MNEDESDWPALPYGDWAETCSALHLWTQIVGKYRLAHTPWVNHSWHATLYVTPRGLTTGMVPDGRSAITLTLDFHDHALIGETADARESFPLEAMSVADFLRRTSTLVERLGGRMDIHGAPNEIADATPFAQDTASRPYDRKAVERFHRALVQIDRVFARFRTGYLGKVSPVHLFWGSFDLAVTRFSGRKAPRHPGGIPALPDPITQEAYSHEVSSAGFWPGGGGVDEAMFYSYAYPAPDGFADRPVEPAAARFDRKLGEFVLPYEAVRASADPEGELTRFLQSTYEAAADLAGWDRQALECDLGVPRIPTGHSNLRGSLNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUR2</accession>
    <name>Q3SUR2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Diaminopimelate decarboxylase</fullName>
            <shortName evidence="2">DAP decarboxylase</shortName>
            <shortName evidence="2">DAPDC</shortName>
            <ecNumber evidence="2 3">4.1.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">lysA</name>
        <name evidence="6" type="ordered locus">Nwi_0713</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Specifically catalyzes the decarboxylation of meso-diaminopimelate (meso-DAP) to L-lysine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>H(+) + meso-2,6-diaminoheptanedioate = CO2 + L-lysine</text>
            <dbReference type="Rhea" id="RHEA:15101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
            <dbReference type="EC" id="4.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 2 3">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 3">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; L-lysine from DL-2,6-diaminopimelate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Orn/Lys/Arg decarboxylase class-II family. LysA subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.20" evidence="2 3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314033.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0713"/>
    <dbReference type="EnsemblBacteria" id="ABA03979">
        <property type="protein sequence ID" value="ABA03979"/>
        <property type="gene ID" value="Nwi_0713"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0713"/>
    <dbReference type="eggNOG" id="COG0019">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026444_0_0_5"/>
    <dbReference type="OMA" id="YCRSMAS"/>
    <dbReference type="OrthoDB" id="861683at2"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00027"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008836">
        <property type="term" value="F:diaminopimelate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06828">
        <property type="entry name" value="PLPDE_III_DapDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02120">
        <property type="entry name" value="LysA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002986">
        <property type="entry name" value="DAP_deCOOHase_LysA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022643">
        <property type="entry name" value="De-COase2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022644">
        <property type="entry name" value="De-COase2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022653">
        <property type="entry name" value="De-COase2_pyr-phos_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000183">
        <property type="entry name" value="Orn/DAP/Arg_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02784">
        <property type="entry name" value="Orn_Arg_deC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00278">
        <property type="entry name" value="Orn_DAP_Arg_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01181">
        <property type="entry name" value="DAPDCRBXLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01179">
        <property type="entry name" value="ODADCRBXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="SSF50621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="SSF51419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01048">
        <property type="entry name" value="lysA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00878">
        <property type="entry name" value="ODR_DC_2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="2 3 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 3" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orn_DAP_Arg_deC" evidence="4">
        <location>
            <begin position="30"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Orn_Arg_deC_N" evidence="5">
        <location>
            <begin position="35"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="2">
        <location>
            <begin position="275"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="318"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="347"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="375"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA03979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="421" mass="45418" checksum="66164510FA092727" modified="2005-10-11" version="1">MHHFDYRDGVLHAEDVDLSTLAEAVGTPFYCYSTATLERHYRVFAEAFAGTDHLICYAVKANSNQSVLRTMARLGAGADVVSGGELKRALAANIPASKILFSGVGKTESELRAALAADVLCINVESEPELDLLSRVAVETGRTARISVRVNPDVDSGTHAKISTGRLENKFGIPITQARAVYARAAKLPGIKVTGADMHIGSQITDLGPMETAFRLLADFVGTLRADGHVISHVDFGGGLGIPYYEDRSAPPEPLAYAEMVRRVTRDLDCTLMFEPGRMIVGNAGVLVTRVIYVKHGNARNFVIIDAAMNDLIRPTLYEAYHQIMPVRAAPRDAAVMVADVVGPVCETGDYLALGRNLPEPQAGDLLAIMTAGAYGAVQSGTYNTRALVPEVLVRGNQYATVRPRIEVDQLIAMDKLAPWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUY6</accession>
    <name>Q3SUY6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0638</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0638"/>
    <dbReference type="EnsemblBacteria" id="ABA03905">
        <property type="protein sequence ID" value="ABA03905"/>
        <property type="gene ID" value="Nwi_0638"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0638"/>
    <dbReference type="HOGENOM" id="CLU_2396673_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="93" mass="10608" checksum="0828AB4D6CFDE874" modified="2005-10-11" version="1">MRFRETRSAAKRQMGGGRRSSLRAIFPRDWLLKRLSEQPDVTLRAARGAGRAKVSYYVVRRFFEHEAISFKKPARRRTGSSRRRAAAGAKVSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV25</accession>
    <name>Q3SV25_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Flagellar M-ring protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Nwi_0599</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">The M ring may be actively involved in energy transduction.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Bacterial flagellum basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the FliF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313927.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0599"/>
    <dbReference type="EnsemblBacteria" id="ABA03866">
        <property type="protein sequence ID" value="ABA03866"/>
        <property type="gene ID" value="Nwi_0599"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0599"/>
    <dbReference type="eggNOG" id="COG1766">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028108_4_1_5"/>
    <dbReference type="OMA" id="NYEVNRI"/>
    <dbReference type="OrthoDB" id="167675at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009431">
        <property type="term" value="C:bacterial-type flagellum basal body, MS ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013556">
        <property type="entry name" value="Flag_M-ring_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000067">
        <property type="entry name" value="FlgMring_FliF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006182">
        <property type="entry name" value="FliF_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043427">
        <property type="entry name" value="YscJ/FliF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30046">
        <property type="entry name" value="PTHR30046"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30046:SF0">
        <property type="entry name" value="PTHR30046:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01514">
        <property type="entry name" value="YscJ_FliF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08345">
        <property type="entry name" value="YscJ_FliF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004862">
        <property type="entry name" value="FliF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01009">
        <property type="entry name" value="FLGMRINGFLIF"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00206">
        <property type="entry name" value="fliF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="14" id="KW-0969">Cilium</keyword>
    <keyword evidence="14" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="429"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="YscJ_FliF" evidence="12">
        <location>
            <begin position="38"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="YscJ_FliF_C" evidence="13">
        <location>
            <begin position="244"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="266"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="468"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="266"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="282"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="316"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR004862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABA03866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="541" mass="58721" checksum="F45C0C203A5B1B56" modified="2005-10-11" version="1">MQGLLAFLKGLGASRLMAMVAVTAVLIGFFAFVIMRVTTPQMTTLFTDLSVEDSSGIIKDLERQAIPFELRNDGAVIMVPKDKVTRLRMKLAEAGLPKGGGVGYEIFDKSDALGTTSFVQNINRLRALEGELARTIRAIDRVQDARVHLVLPERPLFTREAPQPSASIVLRVRGGLEPQQIRAIRHLVASAVNGLKPQRVSIVDETGQLLADGSATDADGVSGDERRAAFEKRMRSQVEAIVSSVVGNGRARVQLSADFDYNKITQTSDKFDPEGRVLRSSQTREESSLTADNNGQVTVNNELPGNQPSDNPNTARDQSKKSEETNNYEISRTTKTEVTEAGRVNRISVAVLVDGAYARNDKGQMVYEERSQEQLDRIATLVRSAIGFDQKRGDQVEVVNLKFAEPPVVVPLAEPTGWLGMMTLTKDDVMYVFELGVMALLGLVVTFMVIRPLVQRILAAEPATPSAQPAAALPVPADASAQPTETGQNLIPGSNATAQLIDVAQVQGQVHAQSVHRVGELAERNPAETASIIRQWLAEPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV62</accession>
    <name>Q3SV62_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0562</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03829">
        <property type="protein sequence ID" value="ABA03829"/>
        <property type="gene ID" value="Nwi_0562"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0562"/>
    <dbReference type="eggNOG" id="ENOG50338KG">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136222_0_0_5"/>
    <dbReference type="OMA" id="FWWLIGT"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021737">
        <property type="entry name" value="Phage_phiKZ_Orf197"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11750">
        <property type="entry name" value="DUF3307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="136" mass="15021" checksum="C6239ADCC850CE93" modified="2005-10-11" version="1">MLLPSLSSSIAVGPLVGWMVVLTVKHVLADFVLQNSWMAVGKDQRTGWALPLLSHCLVHLALALALILVIAPRFWFIAFIDFAIHLVVDRAKGICVSAFDVTQDHQHPWFWTLIGVDQTLHHLTGFGLAIVMAFNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV72</accession>
    <name>Q3SV72_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glycosyl transferase, group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0552</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313880.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0552"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03819">
        <property type="protein sequence ID" value="ABA03819"/>
        <property type="gene ID" value="Nwi_0552"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0552"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054763_0_0_5"/>
    <dbReference type="OMA" id="MEPYRGF"/>
    <dbReference type="OrthoDB" id="694191at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022623">
        <property type="entry name" value="Glyco_trans_4_3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12000">
        <property type="entry name" value="Glyco_trans_4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4_3" evidence="3">
        <location>
            <begin position="30"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="2">
        <location>
            <begin position="211"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="424"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="455" mass="50562" checksum="9D75F6BF82E3C714" modified="2005-10-11" version="1">MPMKILFVHNNFPAQYRHLAAALANEPGFELAAIGASHAHPMPSVKLIKYALPDSDLSGTHPFARRFDSECRRAEQVLYSLSTLATSGFAPDVILAHPGWGETLPLRTMFPKARQLVYCEFFYGAEDRDIGFDPEFPISGLDGHIGLQLKNATTLLALADCDVGISPTNWQRSTFPRHYQSKIEVIHEGVDTSRVRPDAQARLTLPNGREVSRSDEIVTFVVRNLEPLRGYHIFMRALPEILRRRPNAQIVIIGRDGLSYGIPPPPGSTWKSIFLQEVRDRLDMSRVHFLGGVPYNTFLAGLQVSSAHVYLTYPFVLSWSSLEAMSAGCLVIGSDTPPVREIISSGDNGLLVPFFAVDELAERVVEALQEPDRFSAIKEAARQFVIEHFDAKRVCLPRMRQLLDFKPSPPSAAARRKVWSGRAASEWPQATRRSRKVISSPEMEASGEGGSGRTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV82</accession>
    <name>Q3SV82_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0542</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0542"/>
    <dbReference type="EnsemblBacteria" id="ABA03809">
        <property type="protein sequence ID" value="ABA03809"/>
        <property type="gene ID" value="Nwi_0542"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0542"/>
    <dbReference type="eggNOG" id="COG3914">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108867_0_0_5"/>
    <dbReference type="OMA" id="RFRVACF"/>
    <dbReference type="OrthoDB" id="1590420at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="216" mass="24060" checksum="4C9A0CF8659F005A" modified="2005-10-11" version="1">MRASVEPRTNNSGNDALLASPMAATDRTQQLLPRMSSDEAALFLSFVRNSRIYVEFGTGGSTVVASRHVQSSILSVDSSREWLDQVRTACAPNQTQPELIFVDIGPIGEWGFPTDASTKKRWPDYHSAIWKMPESTAADLYFVDGRFRVACFAQIVLHCNPNAIIGIHDFTSRPKYHCVREIAREIATAGDISFFRPLPEKKIAESILQSFHSEPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVD8</accession>
    <name>Q3SVD8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0486</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03753">
        <property type="protein sequence ID" value="ABA03753"/>
        <property type="gene ID" value="Nwi_0486"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0486"/>
    <dbReference type="HOGENOM" id="CLU_2233675_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="105" mass="11567" checksum="8C7F8E28DA9C5384" modified="2005-10-11" version="1">MRRFVSKRDIVAGELRARMARRKKIGTSKLLMRFSTLARACADQVDSDHALETIVRSHFLAAKRSSTSPENTPTVAAPANRPRRRSLPCVIKGGVVAALSAYFVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVJ9</accession>
    <name>Q3SVJ9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Dihydrolipoyl dehydrogenase</fullName>
            <ecNumber evidence="3 13">1.8.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">Nwi_0425</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>(R)-N(6)-dihydrolipoyl-L-lysyl-[protein] + NAD(+) = (R)-N(6)-lipoyl-L-lysyl-[protein] + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:15045"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="EC" id="1.8.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 13">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="11 13">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="13">The active site is a redox-active disulfide bond.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.4" evidence="3 13"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313756.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0425"/>
    <dbReference type="EnsemblBacteria" id="ABA03692">
        <property type="protein sequence ID" value="ABA03692"/>
        <property type="gene ID" value="Nwi_0425"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0425"/>
    <dbReference type="eggNOG" id="COG1249">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016755_0_1_5"/>
    <dbReference type="OMA" id="DAKYGEW"/>
    <dbReference type="OrthoDB" id="267896at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004148">
        <property type="term" value="F:dihydrolipoyl dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006258">
        <property type="entry name" value="Lipoamide_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="SSF55424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01350">
        <property type="entry name" value="lipoamide_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11 13" id="KW-0274">FAD</keyword>
    <keyword evidence="4 13" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7 11 13" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 13 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9 13" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="15">
        <location>
            <begin position="4"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="Pyr_redox_dim" evidence="14">
        <location>
            <begin position="348"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="446"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="144"/>
            <end position="146"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="181"/>
            <end position="188"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="320"/>
            <end position="323"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="12">
        <location>
            <begin position="42"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ABA03692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="467" mass="48822" checksum="D24A159C991795F8" modified="2005-10-11" version="1">MTTYDLIIIGTGPGGYVCAVRAAQLGMKVAVVEKNPTLGGTCLNVGCMPSKALLHASEAFQEAGHSFARMGIGVSAPKLDLSAMMDFKQQGIDGNVKGVEYLMKKNKIDVLRGAGRVVAAGQVEVTGKDGKIQTAETKNIVIATGSDAAKLKGVEIDEKRIVSSTGALSLDKVPEKLLVVGAGVIGLELGSVWRRLGAQVTVVEFLDRILPGMDGEIAKQFQRILEKQGFAFKLGAKVTGVDTSGKTLSARIEPAAGGAAETIEADVVLVAIGRAPYTNGLGLKEAGVALDDRGRVEIDKHFATSVKGIYAIGDVVRGPMLAHKAADEGVACAEILAGQAGHVNYDVIPGVIYTTPEVSSVGKTEEELKQAGTAYAVGKFPFTANGRSKVNQTTDGFVKILADAKTDRVLGVHIIGREAGELIHEACVLMEFGGSAEDLARTCHAHPTRSEAIKEAALAVGKRAIHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVR5</accession>
    <name>Q3SVR5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ATP-dependent helicase HrpB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0359</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0359"/>
    <dbReference type="EnsemblBacteria" id="ABA03626">
        <property type="protein sequence ID" value="ABA03626"/>
        <property type="gene ID" value="Nwi_0359"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0359"/>
    <dbReference type="eggNOG" id="COG1643">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001832_5_6_5"/>
    <dbReference type="OMA" id="DLRILVM"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010225">
        <property type="entry name" value="HrpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013689">
        <property type="entry name" value="RNA_helicase_ATP-dep_HrpB_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08482">
        <property type="entry name" value="HrpB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005496">
        <property type="entry name" value="ATP_hel_hrpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01970">
        <property type="entry name" value="DEAH_box_HrpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="2">
        <location>
            <begin position="44"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="3">
        <location>
            <begin position="231"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="518"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="849"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="518"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="872" mass="94142" checksum="92FBC596A2C03D80" modified="2005-10-11" version="1">MARVRAMRYETGRKAVNHAPRSDRSLTEPFANPLPIDAVLGELGRKLATHDTTVLVAPPGAGKTTRVPLALLDAPWIAGRKIVVLEPRRIAARASAERMARTLGERVGETVGYRVRFGSRVSRGTRIEVVTEGIFSRRILDDPELDGVAAILFDEFHERSLDADLGLALARDVQSGLREDLRILVMSATIDGARVAKLLGNAPVIESKGRAFPVETRYLGRKPDSPVERQMADAIAMALRAEDGSLLAFLPGAAEIRRTQNMLAERIHDASIEIVPLFGALDAAVQDRAIAPAPKGRRKVVLATSIAETSLTIEGVRIVVDSGLARVPRFEPDIGLTRLETMRASRAAVDQRRGRAGRTEPGVCYRLWDEPQTASLAAYTQPEILSADLSSLVLDLAQWGVRDPATLAFLDPPPAPALNEARSLLRELGALDADGRITDEGKSLRALALPPRLARMIVGAHRLGSGREAADIAAVLTERGLGGDSVDLDARLDHFRRDRSPRAVGARQLAERWVSQVAANESVPSSPRGVTSSAPSDRASLPVTGAQGGEELGAGEMLTLAFPDRVARNRGNGSFVLANGRGASVDPASRLAREPFIAVAELTGTAASGRILLAAPISQGQIELRFAGQIETDEDILFDRAAMSLRGRRRKRLRAIMLAEAPVAVAPSIATARILAEGLIDAGLDRLPWSKPLKQWRDRVMFLRAAEGGEWPDLSDAGLAATAETWLAPALSDKTALKEFSAGDLSEALMALLPWNLRARMEREAPTHFEAPTGTMLAIDYEAGQGPTIAVRLQELFGLTAHPSIAQGKVPLVLELLSPAHRPVQVTRDLPGFWRGSYAAVRSDLRGRYPRHPWPDDPANAMPTRRVKPRGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVU1</accession>
    <name>Q3SVU1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Binding-protein-dependent transport systems inner membrane component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0333</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0333"/>
    <dbReference type="EnsemblBacteria" id="ABA03600">
        <property type="protein sequence ID" value="ABA03600"/>
        <property type="gene ID" value="Nwi_0333"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0333"/>
    <dbReference type="eggNOG" id="COG0600">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046113_2_1_5"/>
    <dbReference type="OMA" id="LDIPRMF"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="138"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="183"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="206"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="241"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="75"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="278" mass="29501" checksum="62B55DB9735921E2" modified="2005-10-11" version="1">MTSPSPPAPTGSEASARDSLRPAVRVLLPLAVLGVGVLAWDALVRFKDIPPYVLPGPGLVAATLVQDWPILWRSLLTTLLTTMEGFIAAAIGGVALALLFNQSKWLEQALLPYAIILQVTPVIAIAPLLLIYLPQQTAVVVCAWIVAFFPVLSNTTLGLNSVDRNLAGLFRLYGASRMQTLRYLKLPAALPYILGGLRIAGGLSLIGAVVAEIAAGSAGAGSGLAYRIAESGYRLNIPRMFAALLLLSVAGIVIYMCLAAVSHLMLRRWHESALGKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVX5</accession>
    <name>Q3SVX5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Pilus assembly protein cpaE</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0299</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313632.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0299"/>
    <dbReference type="EnsemblBacteria" id="ABA03566">
        <property type="protein sequence ID" value="ABA03566"/>
        <property type="gene ID" value="Nwi_0299"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0299"/>
    <dbReference type="eggNOG" id="COG4963">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033160_0_0_5"/>
    <dbReference type="OMA" id="MPCIVLD"/>
    <dbReference type="OrthoDB" id="1370117at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_31" evidence="2">
        <location>
            <begin position="159"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="421" mass="45247" checksum="844C2AF531B27C07" modified="2005-10-11" version="1">MISYARNDADSTTSPSPRAEDHIAPAPRVSVQAFCETMETATAVQAASEDRRLAKAHLKIQMGGMTAAVEAYRSAPTPNVIILETEGHGDILAALDHLATVCDAGTRVIVIGRVNDVTLYRELVKRGVSDYLMAPAAPIDIVRSVCGLFSTPEAKAVGRIIAIAGAKGGVGASTIAHNVAWAIARDLALDSVVADLDLAFGTAGLDFNQDPPQGIADAVFSPDRIDTAFVDRLLSKCTDHLSLLAAPATLDRVYDFGTEAFDSTLDTLRATMPCIVLDIPHQWSGWTRRALIGADDILIVATPDLANLRNTKNLFDLLKVARPNDRPPLYCLNQVGVPKRPEISGGEFAKAIESQPVVSIPFDPHMFGSAANNGQMIAEIAPNHRATEMFLQIAQRLTGRGEAKKPRKSFLPPFIEKLRAR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVY8</accession>
    <name>KDSB_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-deoxy-manno-octulosonate cytidylyltransferase</fullName>
            <ecNumber evidence="1">2.7.7.38</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">CMP-2-keto-3-deoxyoctulosonic acid synthase</fullName>
            <shortName evidence="1">CKS</shortName>
            <shortName evidence="1">CMP-KDO synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">kdsB</name>
        <name type="ordered locus">Nwi_0286</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3-deoxy-alpha-D-manno-oct-2-ulosonate + CTP = CMP-3-deoxy-beta-D-manno-octulosonate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23448"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:85986"/>
            <dbReference type="ChEBI" id="CHEBI:85987"/>
            <dbReference type="EC" id="2.7.7.38"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; CMP-3-deoxy-D-manno-octulosonate biosynthesis; CMP-3-deoxy-D-manno-octulosonate from 3-deoxy-D-manno-octulosonate and CTP: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the KdsB family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.38" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313619.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVY8"/>
    <dbReference type="SMR" id="Q3SVY8"/>
    <dbReference type="STRING" id="323098.Nwi_0286"/>
    <dbReference type="EnsemblBacteria" id="ABA03553">
        <property type="protein sequence ID" value="ABA03553"/>
        <property type="gene ID" value="Nwi_0286"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0286"/>
    <dbReference type="eggNOG" id="COG1212">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065038_0_1_5"/>
    <dbReference type="OMA" id="FMATCAK"/>
    <dbReference type="OrthoDB" id="1345588at2"/>
    <dbReference type="UniPathway" id="UPA00030"/>
    <dbReference type="UniPathway" id="UPA00358">
        <property type="reaction ID" value="UER00476"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008690">
        <property type="term" value="F:3-deoxy-manno-octulosonate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033468">
        <property type="term" value="P:CMP-keto-3-deoxy-D-manno-octulosonic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009103">
        <property type="term" value="P:lipopolysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02517">
        <property type="entry name" value="CMP-KDO-Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00057">
        <property type="entry name" value="KdsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003329">
        <property type="entry name" value="Cytidylyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004528">
        <property type="entry name" value="KdsB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02348">
        <property type="entry name" value="CTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00466">
        <property type="entry name" value="kdsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0448">Lipopolysaccharide biosynthesis</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000370108" description="3-deoxy-manno-octulosonate cytidylyltransferase">
        <location>
            <begin position="1"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00057"/>
        </source>
    </evidence>
    <sequence length="254" mass="27425" checksum="A90B64C9CAB278D7" modified="2005-10-11" version="1">MTENRTLVLIPARMAATRLPGKPLLDIGGLPMIVHVLRRAEAAGIGRVAVATDTSDIATAVTAHGGEAIMTRADHPSGSDRVFEALGKLDPEGRIDTVVNLQGDFPTIRPGTIREVLQPLADNAVDIATLAAEIHTEEEAANPNVVKAVGSPIGPRRLRALYFTRATAPHGEGPRYHHVGLYAYRREALQRFIELPPSPLEQQERLEQLRALEGGMRIDIMIVDDVPRGVDTATDLETARQIIACSASGEHPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW17</accession>
    <name>Q3SW17_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0257</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313590.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0257"/>
    <dbReference type="EnsemblBacteria" id="ABA03524">
        <property type="protein sequence ID" value="ABA03524"/>
        <property type="gene ID" value="Nwi_0257"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0257"/>
    <dbReference type="eggNOG" id="COG4916">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_665354_0_0_5"/>
    <dbReference type="OMA" id="EMEGMAF"/>
    <dbReference type="OrthoDB" id="1201025at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003953">
        <property type="term" value="F:NAD+ nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019677">
        <property type="term" value="P:NAD catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13676">
        <property type="entry name" value="TIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="SSF52200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TIR" evidence="1">
        <location>
            <begin position="95"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="404" mass="45229" checksum="0AE69A8F6DD6588D" modified="2005-10-11" version="1">MIYFEINGKRIKPGDFGDALMQAVLKSLEAQIREKIGSIRDPETGEFPVVVMRGDSLENLKMHVEGSPELIALVRERMGDGEEEETPDENAMPEGAPRVFLSYTSDDAALAERIATALQANGIDTWWDKWCITTGDSLRQKIDEGLGDCTHFLVLLTPQSVGKPWVNQEMDAGLVRKLGNKCRFLPVRHKLPPKDLPPLLSGMHAPEISADEDITQLINDIHGISRKPRLGPAPHAVAAEAVTQTGCSAAANTIARYFVENTTYGRFADPIIDVEPLAEKTGLTIADTKDALYELSAFFKDTKIHALVKASLFTEFDRHWKPWDPREDALKLAADIMNDPKFPAESKAIAERYGWEPRRLNPAANYLFERGLLVDYKVIGDREFELHRIVGKADQLRRFLKGRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW31</accession>
    <name>Q3SW31_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 13">5-aminolevulinate synthase</fullName>
            <ecNumber evidence="6 13">2.3.1.37</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 13">5-aminolevulinic acid synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10 13">Delta-ALA synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11 13">Delta-aminolevulinate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">Nwi_0242</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>glycine + H(+) + succinyl-CoA = 5-aminolevulinate + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:12921"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="2.3.1.37"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from glycine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.37" evidence="6 13"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313576.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0242"/>
    <dbReference type="EnsemblBacteria" id="ABA03510">
        <property type="protein sequence ID" value="ABA03510"/>
        <property type="gene ID" value="Nwi_0242"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0242"/>
    <dbReference type="eggNOG" id="COG0156">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015846_11_1_5"/>
    <dbReference type="OrthoDB" id="479874at2"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00375"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003870">
        <property type="term" value="F:5-aminolevulinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010961">
        <property type="entry name" value="4pyrrol_synth_NH2levulA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01821">
        <property type="entry name" value="5aminolev_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13 15" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7 13" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="14">
        <location>
            <begin position="46"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU910713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABA03510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="409" mass="44858" checksum="B7E96583FAAB8483" modified="2005-10-11" version="1">MDYSKFFCNALDRLHDERRYRVFADLERIAGRFPYATLHTPQGPRNVIIWCSNDYLGMGQHPKVVGAMVETATRVGTGAGGTRNIAGTHHPLVQLEQELADLHGKPAALVFTSGYVSNQTGISTLAKLIPDCLILSDALNHNSMIEGIRQSGCERVVFRHNDLAHLEELLRAAPPDRPKLIACESLYSMDGDVAPLSKICDLAERYGAMTYVDEVHAVGMYGPRGGGIAEREGVMHRIDVLEGTLAKAFGCLGGYIAARSEIIDAVRSYAPGFIFTTSLPPAICSAATAAIRHLKSSTWERERHQERAARTKASLIAAGLPVMVSSTHIVPVFVGDPERCKRASDMLLHDHNIYIQPINYPTVAKGMERLRITPSPYHDDALVDQLAEALLQVWERLGLPLQAKSMAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW67</accession>
    <name>Q3SW67_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">30S ribosomal protein S1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0206</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">Binds mRNA; thus facilitating recognition of the initiation point. It is needed to translate mRNA with a short Shine-Dalgarno (SD) purine-rich sequence.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the bacterial ribosomal protein bS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313542.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0206"/>
    <dbReference type="EnsemblBacteria" id="ABA03474">
        <property type="protein sequence ID" value="ABA03474"/>
        <property type="gene ID" value="Nwi_0206"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0206"/>
    <dbReference type="eggNOG" id="COG0539">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015805_2_1_5"/>
    <dbReference type="OMA" id="SLNEFRY"/>
    <dbReference type="OrthoDB" id="1235756at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035104">
        <property type="entry name" value="Ribosomal_protein_S1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000110">
        <property type="entry name" value="Ribosomal_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10724:SF7">
        <property type="entry name" value="PTHR10724:SF7"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002111">
        <property type="entry name" value="RpsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00681">
        <property type="entry name" value="RIBOSOMALS1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00717">
        <property type="entry name" value="rpsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
            <begin position="30"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
            <begin position="114"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
            <begin position="201"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
            <begin position="286"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
            <begin position="373"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
            <begin position="454"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR002111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="567" mass="62889" checksum="B73B03BE150D1F39" modified="2005-10-11" version="1">MASTDTLNPSRDDFAAMLDESFAGGSLQESSVIKGKVVAIEKDMAVIDVGLKTEGRVALREFSGPGRESTLKVGDEVEVFLDRIENALGEAVLSRDKARREESWGKLEKAFNNNEKVHGVIFNQVKGGFTVDLDGAVAFLPRSQVDIRPIRDVGPLMNNTQPFQILKMDRRRGNIVVSRRTVLEETRAEQRQELVQNLEEGQVIDGVVKNITDYGAFVDLGGIDGLLHVTDIAWRRVNHPTEVLAIGQTVKVKIIKINHETHRISLGMKQLLDDPWQGIEAKYPLGARFTGRVTNITDYGAFVELEPGIEGLIHVSEMSWTKKNMHPGKIVSTSQEVEVQVLEVDSVKRRISLGLKQTMRNPWEAFIEKHPVGSTVEGEVKNKTEFGLFLGLEGDVDGMVHLSDLDWKQPGEQVIDNFKRGDMVKAVVLDVDVEKERISLGVKQLEGDPFAEPGDVKKGAVVTCEVLEVKDGGIEVQISGTEFQTFIKRSELARDRNDQRTERFAVGEKVDARVIQFDKKARKVQVSIKALEVAEEKEAIAQYGSSDSGATLGDILGTVLKQRTDNK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW76</accession>
    <name>DNAK_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chaperone protein DnaK</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">HSP70</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Heat shock 70 kDa protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Heat shock protein 70</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dnaK</name>
        <name type="ordered locus">Nwi_0197</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as a chaperone.</text>
    </comment>
    <comment type="induction">
        <text evidence="1">By stress conditions e.g. heat shock.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313533.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SW76"/>
    <dbReference type="SMR" id="Q3SW76"/>
    <dbReference type="STRING" id="323098.Nwi_0197"/>
    <dbReference type="PRIDE" id="Q3SW76"/>
    <dbReference type="EnsemblBacteria" id="ABA03465">
        <property type="protein sequence ID" value="ABA03465"/>
        <property type="gene ID" value="Nwi_0197"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0197"/>
    <dbReference type="eggNOG" id="COG0443">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005965_2_1_5"/>
    <dbReference type="OMA" id="DKMVLQR"/>
    <dbReference type="OrthoDB" id="161217at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00332">
        <property type="entry name" value="DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012725">
        <property type="entry name" value="Chaperone_DnaK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="PTHR19375"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="SSF100920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="SSF100934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02350">
        <property type="entry name" value="prok_dnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0346">Stress response</keyword>
    <feature type="chain" id="PRO_0000225985" description="Chaperone protein DnaK">
        <location>
            <begin position="1"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="516"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="516"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="541"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphothreonine; by autocatalysis" evidence="1">
        <location>
            <position position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="630" mass="67888" checksum="17ECFD0AB7A43AE0" modified="2005-10-11" version="1">MGKVIGIDLGTTNSCVAVMDGKTPKVIENAEGMRTTPSIVAFSDDGERLVGQPAKRQAVTNPERTIFAVKRLIGRRYDDPTVEKDKQLVPYKIAKAGNGDAWVEADGKTYSPSQISAFTLQKMKETAEAHLGQKVDQAVITVPAYFNDAQRQATKDAGKIAGLEVLRIINEPTAAALAYGLDKSKAGTIAVYDLGGGTFDVSVLEIGDGVFEVKSTNGDTFLGGEDFDMRLVNYLADEFQKEQGINLRNDKLALQRLKEAAEKAKIELSSTTQTEINLPFITADQSGPKHLTMKLTRAKFEALVDDLVQKTIEPCRKALKDAGLTAGEIGEVVLVGGMTRMPKVQEVVKQLFGKEPHKGVNPDEVVAIGAAIQAGVLQGDVKDVLLLDVTPLSLGIETLGGVFTRIIDRNTTIPTKKSQVFSTAEDNQNAVTIRVFQGEREMAADNKILGQFDLMGIPPAPRGMPQIEVTFDIDANGIVNVSAKDKATGKEQQIRIQASGGLSEADIEKMVKDAEANAAEDKKRREAVDAKNQADSLVHSTEKALTEHGSKIEESERRAIEDAVSDLKEALKGDDAEAIKAKTNTLAQASMKLGEAMYKQQAEADAAKDAAKDDVVDAEFTEVDDDKKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW89</accession>
    <name>Q3SW89_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_0184</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0184"/>
    <dbReference type="EnsemblBacteria" id="ABA03452">
        <property type="protein sequence ID" value="ABA03452"/>
        <property type="gene ID" value="Nwi_0184"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0184"/>
    <dbReference type="eggNOG" id="COG0730">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045498_0_0_5"/>
    <dbReference type="OMA" id="LCITRAI"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="38"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="78"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="210"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="306"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA03452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="343" mass="36268" checksum="072BD2EF36289214" modified="2005-10-11" version="1">MISVMQGTRLAAVPRPEVNAVKAPPVSSQIRKRARVQLYLPIADIPVNVLLILAMGAAVGFVSGMFGIGGGFLMTPLLIFVGIAPAVAVATVTSHIAASSFSGALSYWRKRAVDPMLALVLLSGGVIGTILGVLTFTRLRSLGQLDLLIALSYLVLLSGVGAAMSWEGLRAMLRARQNRTVPLRRSGGHGWVHGLPLKIRFKRSKIYLSAIPVIAIGLLIGFIGAVMGIGGGFILVPLLIYLLRVPTSTVIGTSMMLTLITMTFATVLHAATNHLVDAVLALILMTGGVTGAQFGARAGQRIRGEHLRLLLGMLILAVGIRFAIELGLRPADLYTVRETTGDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWA4</accession>
    <name>Q3SWA4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH OST-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0169</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313506.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0169"/>
    <dbReference type="EnsemblBacteria" id="ABA03437">
        <property type="protein sequence ID" value="ABA03437"/>
        <property type="gene ID" value="Nwi_0169"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0169"/>
    <dbReference type="eggNOG" id="COG1432">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034061_0_2_5"/>
    <dbReference type="OMA" id="IDADNTQ"/>
    <dbReference type="OrthoDB" id="990092at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041966">
        <property type="entry name" value="LOTUS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021139">
        <property type="entry name" value="NYN_MARF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025605">
        <property type="entry name" value="OST-HTH/LOTUS_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01936">
        <property type="entry name" value="NYN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12872">
        <property type="entry name" value="OST-HTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51644">
        <property type="entry name" value="HTH_OST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH OST-type" evidence="2">
        <location>
            <begin position="171"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="271" mass="30153" checksum="2E30A4519461D845" modified="2005-10-11" version="1">MKAEPRQRLPRFAVFIDAENVPPKFADGIFREIAQLGDAPVRLIYGNLSDPNLKGWTEVLPDHSLERRDPAVKGRNSADMAIVIDAMDLLHDGRIHGFCLISSDSDFTGLAARLRREGANVYGFGEKKTPDCFRRACNRFISLESLLPRKPARKPIAVSPSILPNSNALKPPSDAVPILNKALSRIESEDGWARLGQVGQQIPKLFSDFDIRTYGFSKLSRLVRKTGAFEIDENNGPIRIRRAKRWNPTTPGRQVQDLSLSAGSSARQRPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWB7</accession>
    <name>Q3SWB7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">tRNA and rRNA cytosine-C5-methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0156</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313493.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0156"/>
    <dbReference type="EnsemblBacteria" id="ABA03424">
        <property type="protein sequence ID" value="ABA03424"/>
        <property type="gene ID" value="Nwi_0156"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0156"/>
    <dbReference type="eggNOG" id="COG0144">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005316_0_4_5"/>
    <dbReference type="OMA" id="RVNRQHH"/>
    <dbReference type="OrthoDB" id="1064993at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB/NOP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035926">
        <property type="entry name" value="NusB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006027">
        <property type="entry name" value="NusB_RsmB_TIM44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="PTHR22807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01029">
        <property type="entry name" value="NusB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48013">
        <property type="entry name" value="SSF48013"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM_MT_RSMB_NOP" evidence="6">
        <location>
            <begin position="164"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="5">
        <location>
            <begin position="259"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="378"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="450" mass="48227" checksum="1AA680455302BCF2" modified="2005-10-11" version="1">MPSTRFAPPTEAPGLAARRIAADILDGVLHKRRTLDEQLEGPTAHPGLKALADRDRALMRRLVATILRRLGTLGHLLSRLLDRGIPTDAPRAQSALLIGAAQILWMDVPDHAAVDLSVRLVQSDRRAAKYAGLVNAVLRRCAREGQSLIEEVRSESLDTPSWLMARWSGYYGEGTAKAIAAAISHEPALDLTVKANAAHWATRLHGETLPTGTVRTSLHGSVTMLPGFSEGQWWVQDAAAALPARLFGDLRGRRVIDLCAAPGGKTAQLVQAGAEVTAIDRSPNRVARLRENLGRLSLDARSLVADAIEWQDDVAGDGFDGVLVDAPCSATGTIRRHPDVAWLKQDSDIAALTALQQRLLNKAITLLRPGGTLVYCTCSLEPEEGEQVVAALLATDTGIRRVPVSADEVAGLADIVTPEGDLRTLPCHLAHDDPTLGGLDGFYAARLTRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWE1</accession>
    <name>Q3SWE1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidoglycan glycosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.129</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">Nwi_0132</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = [GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23708"/>
            <dbReference type="Rhea" id="RHEA-COMP:9602"/>
            <dbReference type="Rhea" id="RHEA-COMP:9603"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:60033"/>
            <dbReference type="ChEBI" id="CHEBI:78435"/>
            <dbReference type="EC" id="2.4.1.129"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the transpeptidase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the glycosyltransferase 51 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.129" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313469.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0132"/>
    <dbReference type="CAZy" id="GT51">
        <property type="family name" value="Glycosyltransferase Family 51"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03400">
        <property type="protein sequence ID" value="ABA03400"/>
        <property type="gene ID" value="Nwi_0132"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0132"/>
    <dbReference type="eggNOG" id="COG0744">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006354_2_4_5"/>
    <dbReference type="OMA" id="AVQMPYI"/>
    <dbReference type="OrthoDB" id="652304at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008658">
        <property type="term" value="F:penicillin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008955">
        <property type="term" value="F:peptidoglycan glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3810.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001264">
        <property type="entry name" value="Glyco_trans_51"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036950">
        <property type="entry name" value="PBP_transglycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001460">
        <property type="entry name" value="PCN-bd_Tpept"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00912">
        <property type="entry name" value="Transgly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00905">
        <property type="entry name" value="Transpeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="7 15" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="81"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Transgly" evidence="14">
        <location>
            <begin position="133"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Transpeptidase" evidence="13">
        <location>
            <begin position="389"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="34"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="661"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="34"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="695"/>
            <end position="710"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABA03400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="734" mass="78764" checksum="0A036E9C10D72CE0" modified="2005-10-11" version="1">MALGQKKRSGRKEPVFSGFAASLTELRLNPLDRVTIADDKPARRTPPRKKATAPRSEAPRKPPGGRKGQAKKRKRAGLSRLFYWSAVVALWSVIAVVGVLIYVGAHLPPIQSLEIPKRPPTIQIVAADGSVLATRGEMAGSNVALKELPSYLPNAFIAIEDRRFHSHYGVDPLGILRAAVANVLHRGVSQGGSTLTQQLAKNLFLTQERTLERKLQEVELALWLERKHTKAQILELYLNRVYFGSGAYGIEAAAQKYFGKPAKNVTLAEAAMLAGLVKSPSRLAPDRNPEGAEARAKIVLAAMADAHLITGQQARAATAKPSYAMKPASAGTANYVADWVGEVLDDLVGQVDQNIIVETTIDPKLQSAAEAALIDELAAKSIKFNVSQGALVAMTPDGAIRAMVGGRNYADSQFNRATMAKRQPGSAFKPFVYLAAIEAGLTPETIRQDAPLDIKGWRPENYTHEYFGAVTLTQALAMSLNTVAVRLGLEVGAGKVVRTAHRLGITSKLEPNASIALGTSEVSPLELVGAFAPFANGGYAAGPHVVERVRTAGNNKLLYARGKERPDRVIDPRAVAMMNTMMEQTLINGTARKAEIPGWTAAGKTGTSQDFRDAWFIGYTARLVTGVWLGNDDNTPTRKATGGSLPVEVWSRFMRAAHQGVRPEPIPGTQRSGFPPEVAQFGQPADAPVRAASPTLGRSPPPPIPQPARADARPQPASGLGLDGWLNDRLFGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWK3</accession>
    <name>Q3SWK3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hydrolase_4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313407.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0070"/>
    <dbReference type="EnsemblBacteria" id="ABA03338">
        <property type="protein sequence ID" value="ABA03338"/>
        <property type="gene ID" value="Nwi_0070"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0070"/>
    <dbReference type="eggNOG" id="COG1073">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066961_0_0_5"/>
    <dbReference type="OMA" id="TISRWLE"/>
    <dbReference type="OrthoDB" id="721431at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydrolase_4" evidence="1">
        <location>
            <begin position="56"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="257" mass="27939" checksum="5CC26480F4AD4921" modified="2005-10-11" version="1">MTDGTATQFIEVGGGDAARRIAVRARRGGAAGLFWLGGFRSDMAGTKALALDAWAAEHGRACVRFDYSGHGESGGEFTEGTISRWLEESLAVFDAFCEGPQVVIGSSMGGWIALLLARAVARRASSRATLSGLVLIAPAPDFTEELMWKAFPPEARHALETHGVWLRPSDYGDPYPITRKLIEDGRNHLLLGKSISTGCPVRILQGAQDTDVPWRHAFALTERLPSDDVVLTMIRDGDHRLSRPQDIARILAAVREF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWK6</accession>
    <name>RL20_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplT</name>
        <name type="ordered locus">Nwi_0067</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009796601.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWK6"/>
    <dbReference type="SMR" id="Q3SWK6"/>
    <dbReference type="STRING" id="323098.Nwi_0067"/>
    <dbReference type="EnsemblBacteria" id="ABA03335">
        <property type="protein sequence ID" value="ABA03335"/>
        <property type="gene ID" value="Nwi_0067"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0067"/>
    <dbReference type="eggNOG" id="COG0292">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123265_0_1_5"/>
    <dbReference type="OMA" id="GRRKNVW"/>
    <dbReference type="OrthoDB" id="1932144at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07026">
        <property type="entry name" value="Ribosomal_L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1900.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00382">
        <property type="entry name" value="Ribosomal_L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005813">
        <property type="entry name" value="Ribosomal_L20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035566">
        <property type="entry name" value="Ribosomal_protein_L20_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10986">
        <property type="entry name" value="PTHR10986"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00453">
        <property type="entry name" value="Ribosomal_L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00062">
        <property type="entry name" value="RIBOSOMALL20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74731">
        <property type="entry name" value="SSF74731"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01032">
        <property type="entry name" value="rplT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00937">
        <property type="entry name" value="RIBOSOMAL_L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000243706" description="50S ribosomal protein L20">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00382"/>
        </source>
    </evidence>
    <sequence length="119" mass="13234" checksum="570C87CCA789A913" modified="2005-10-11" version="1">MARVKRGVTAHAKHKKVYKVTKGFSGRRKNTIRAAKAAADKAGQYAFRDRKRKKRTFRALWIQRLNAAVRPFGMTYSRFIDGLSKSGITVDRKVLSDLAINEPAAFQAIAEKAKAALAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWL6</accession>
    <name>Q3SWL6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0057</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313396.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0057"/>
    <dbReference type="EnsemblBacteria" id="ABA03325">
        <property type="protein sequence ID" value="ABA03325"/>
        <property type="gene ID" value="Nwi_0057"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0057"/>
    <dbReference type="eggNOG" id="COG5416">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154612_0_0_5"/>
    <dbReference type="OMA" id="CVANRQS"/>
    <dbReference type="OrthoDB" id="1969603at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="115" mass="12910" checksum="8F79625F5B7D2E65" modified="2005-10-11" version="1">MRKFLTGLILIPLGVIFIVFAFANRRLVTVTFDPFGGSDPQTGVTLPLFILIISVAIFGVVAGSVATWVGQRRWRYAARQYESDARKAHVELADLRSRYESQEVTRLPELTRRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWM3</accession>
    <name>Q3SWM3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Nwi_0050</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0050"/>
    <dbReference type="EnsemblBacteria" id="ABA03318">
        <property type="protein sequence ID" value="ABA03318"/>
        <property type="gene ID" value="Nwi_0050"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0050"/>
    <dbReference type="eggNOG" id="COG1074">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001114_0_0_5"/>
    <dbReference type="OMA" id="KQSIYRW"/>
    <dbReference type="OrthoDB" id="137860at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014151">
        <property type="entry name" value="DNA_helicase_AddA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038726">
        <property type="entry name" value="PDDEXK_AddAB-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="PTHR11070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12705">
        <property type="entry name" value="PDDEXK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00580">
        <property type="entry name" value="UvrD-helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02784">
        <property type="entry name" value="addA_alphas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51217">
        <property type="entry name" value="UVRD_HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="9 13" id="KW-0347">Helicase</keyword>
    <keyword evidence="9 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="11">
        <location>
            <begin position="9"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="UvrD-like helicase C-terminal" evidence="12">
        <location>
            <begin position="521"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="967"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1008"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="30"/>
            <end position="37"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA03318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1202" mass="132187" checksum="2E6CD67C6ACBCD9F" modified="2005-10-11" version="1">MVKAPRPVHPDASARQQRASDPATSVFVSANAGSGKTHVLVQRVIRLLLNDVDPARILCITFTKAAAANMAERVFSTLGHWVTLDDEALNGALRDVGIAQPGARWRERARKLFASALETPGGLKVQTIHALCTRLLQQFPFEAEVPARFTVLDERDQTEMMERASLKVMLDASQDPDSPSGLALRYAMSAATDSTLRDVLNQACLSRDHALTQTDDRGIAQAIRDAADALGVDPAERVEDVEREIVDGPNLPRSKWLDTAALIATGSSNDVKQAERLRAAHAEAGDAARADRYLDVFLTGDGSPRKSFVTRKISDARPDVAEMLADECQRVIALLGRRRAINIRDRTQSLLVIAAAVAAHYRREKHERGLLDYDDLIDKTLRMLEQTSPGWVHYKLDRGVDHVLIDEAQDTSPKQWDIVERIIADFTTGEGAREGVRRTVFAVGDEKQSIFSFQGAAPREFDERRRKLERKFTDAKLPFRKEDFIYSFRSGKTILESVDHVFRDPAIYASIHAVGAHPVHESLADAAPGIVDLWSLEERDKREEMEGWRAPFDAVTATSPEVKLARRIRDEIKSLIAQGAMTGHLGNRRPLGYGDILVLVRRRGNAFDAVIQALKQSGIPVAGADRLKLTEHIAIIDLMNLADALLLPRDDLALAVALKSPLFGLDDDDLFKLAWQRKGTLRDALAGHATADDKFRDAFESLKRCESRAARDTPFAFFAWLLGEEAGRRRILRRLGHEANDALDEFLELALTCERKAPASLQGFMAWLRAADTEVKRDMEISRDEVRVMTVHGAKGLEASVVFLADTSTSPADTERLSLIRMGRGNAPAGVAGVTIWAGRKAEDPPCVAQARAAMTAETEHEYRRLLYVAMTRAADRLIVGGCKPANRKDVREFSWYHLIEKGLANSGLAMQEIETASGKVKRFTRPDHDDPGSNRSRIMNVIDSNILERDAGGKPLHTFPHPALEEAEPDAAGPAPPASPGATRIPLPPWLHLAAAPEAPARRSLYPSDADEAAHQRAPAGAIPDQRHRARLRGTLVHRLLQSLPEVPRDRRREAALSYLGRNASGWGKTEHDALAARVVAVLDDARFAPVFAEGSRAEVAIVGRLTTRRGDPLPVSGQIDRLVVTPHGVLVVDYKTSPAPASLADVPPAYIRQLALYRAMLAGLYPELPVNAALLWTETPEMMEISASTLDAALEAIISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWQ6</accession>
    <name>Q3SWQ6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">CTransporter-associated region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0017</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0053 family. Hemolysin C subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313356.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0017"/>
    <dbReference type="EnsemblBacteria" id="ABA03285">
        <property type="protein sequence ID" value="ABA03285"/>
        <property type="gene ID" value="Nwi_0017"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0017"/>
    <dbReference type="eggNOG" id="COG1253">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015237_3_1_5"/>
    <dbReference type="OMA" id="QMISIKA"/>
    <dbReference type="OrthoDB" id="1155802at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04590">
        <property type="entry name" value="CBS_pair_CorC_HlyC_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044751">
        <property type="entry name" value="Ion_transp-like_CBS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005170">
        <property type="entry name" value="Transptr-assoc_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03471">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01091">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0129">CBS domain</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="6">
        <location>
            <begin position="93"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="6">
        <location>
            <begin position="185"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="333"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="333"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="375" mass="41183" checksum="BA1E515246437D27" modified="2005-10-11" version="1">MPSEDQTSESPPAQESRNLPVPVHNGEVLRPAPERWLMRAIRSLFGWKAGSVRADLQVVLDASTPDETSFTAVERTMLRNILNLHERRIADVMVHRADIVAVKRDIPLGELINLFESAAHSRLVVYHENLDDPEGMVHIRDLLAFMAQRARTAMPSTAKRKKKTPPAGLDLRAIDLALPLSEADITRKLLYVPPSMPAIDLLAQMQASRIHLALVVDEYGGTDGLVSIEDIVEQVVGEIDDEHDSDEPPSIVRQTENSFIADARASLEDVRAMIGEAFETGEAGEEVDTLGGFLVTYVGRLPVRGEVILGPGPFEIEVLDADPRRVKRLRIGLRAERPPVRPRDGRRRENAPDATSQSQGDPASPSSPGDGGDPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMW4</accession>
    <name>Q3SMW4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Secretion protein HlyD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_3130</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316285.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3130"/>
    <dbReference type="EnsemblBacteria" id="ABA06377">
        <property type="protein sequence ID" value="ABA06377"/>
        <property type="gene ID" value="Nwi_3130"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3130"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018816_13_1_5"/>
    <dbReference type="OMA" id="PDETSHE"/>
    <dbReference type="OrthoDB" id="949250at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043602">
        <property type="entry name" value="CusB_dom_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032317">
        <property type="entry name" value="HlyD_D23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006143">
        <property type="entry name" value="RND_pump_MFP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00529">
        <property type="entry name" value="CusB_dom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16576">
        <property type="entry name" value="HlyD_D23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01730">
        <property type="entry name" value="RND_mfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CusB_dom_1" evidence="3">
        <location>
            <begin position="19"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="HlyD_D23" evidence="4">
        <location>
            <begin position="173"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA06377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="473" mass="50561" checksum="35FE5ACE3B74196A" modified="2005-10-11" version="1">MKPLALMGLALAAILAAGAGGYWAGVRNLPITGLREWLGVEATFAAAEPAGTGAVIYYQDPDGKPAYSATPRKTADGRAFRAVRASEDVSFEEKQPTTAEATGQPGARRVLYYRNPMGLPDTSPTPKKDSMGMDYIPVYEGEDDGGSVVRVSLGKLQRTGVRSELVKRRTVGRLMRVPGMIQLDERRISVVATRSDAFVNEVANVTTGDRVTKGQVLVRLYSPDIAAAGALFLSDLNIGGRDGAIGGARQRLENLGVPPEAIAEIERTRKVPLSITWRAPRDGVVLERNVVEGMKAAPGDVLFRLADISTIWVLADVPEFDLAAVRIGAPVTVRVRSLPGRSFEGRVSLIYPQVGEATRTARVRIEIANPDGVLLPNMYAEVEIGTGDAAPVVAVPDSAVIDTGTRQVVIIDRGDGRFEPRDVKIGLRGEGLTEIREGIAEGDRVVVAANFLIDAESNLKAALRGLTPPEARP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN05</accession>
    <name>Q3SN05_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_3089</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3089"/>
    <dbReference type="EnsemblBacteria" id="ABA06336">
        <property type="protein sequence ID" value="ABA06336"/>
        <property type="gene ID" value="Nwi_3089"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3089"/>
    <dbReference type="eggNOG" id="COG1475">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_749715_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="329"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="174"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="369" mass="41312" checksum="D6C38F1485F6CD68" modified="2005-10-11" version="1">MVDGGMSTEEVASTYFTSQRYVDQRLALAKVSPRLHEVYAQDGMTLAMLEAFTAHPDHARQEQVWDAVERSHYREPWRIRNMLTETTVAASDKRALFVGIEAYMAAGGQMLPRYLFDDDEDGWLKDVSLLDRLVAEKLKVVADEVAAEGWKWITVDLELPYGHDHGLRAITGAFAELTRKEQRQREKLREEQERLETEYADYDELPDDIDQRLGEIEKQLDAFERRPAVYDPAEIAIAGVFITVDEDGALARVTGLDMADAGFRPTVANYLGRITKPRILQAVHEALGEEKVRLIDHLKKDDMAREAERLLADTGWLPEPLRLVAEAPAAETSAGQNGDDVALPEFFADDDDGAADEATGDPTPLIAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNA5</accession>
    <name>Q3SNA5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulator, XRE family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_2986</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain fatty acyl-CoA assimilation regulator (ScfR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316158.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2986"/>
    <dbReference type="EnsemblBacteria" id="ABA06236">
        <property type="protein sequence ID" value="ABA06236"/>
        <property type="gene ID" value="Nwi_2986"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2986"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3800">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046383_0_0_5"/>
    <dbReference type="OMA" id="RRFDRHS"/>
    <dbReference type="OrthoDB" id="195775at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026281">
        <property type="entry name" value="HTH_RamB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010359">
        <property type="entry name" value="IrrE_HExxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018653">
        <property type="entry name" value="ScfR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06114">
        <property type="entry name" value="Peptidase_M78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09856">
        <property type="entry name" value="ScfRs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019251">
        <property type="entry name" value="Rv0465c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="5">
        <location>
            <begin position="15"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA06236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="483" mass="54472" checksum="D04F1BD431239A02" modified="2005-10-11" version="1">MASDPGKKLFVGPRFRRVRQQLGLSQTQFAEGLGISPSYVNLIERNQRPVTAQILLRLAETYDLDLRDLATADEDRFFAELNEVFSDPLFRQIDIPRQELRDLAELCPGVTHALQRLYAAYTEARRGETLAAAQFAGRDDSTGARFEADPIERVRDLIEANRNYFPELEQAAEALRDEIDVPAQDLFAALSARLREKHSIQTRIMPVDIMRETLRRFDRHRRQLLISELVDGPGRAFQLAFHIALAECTPVLDAIIARAGALDDTSRQLYRITLANYFAGCALMPYQPFLAAAESLGYDLHVLAQRFSTGFEQVCHRLTTLQRPNARGVPFFMLRVDNAGNVSKRFSSGTFPFSKFGGTCPLWNVHSTFDTPDRLLKQVIELPDGTRYFSVAQMVRRPVAPHPLAQPRFAIGLGCEIRHAARLVYASGMNLDNAEGTPIGINCRLCERENCAQRADPPLTRTLILDENTRRMTSFAFSNAREV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNF4</accession>
    <name>Q3SNF4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2937</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2937"/>
    <dbReference type="EnsemblBacteria" id="ABA06187">
        <property type="protein sequence ID" value="ABA06187"/>
        <property type="gene ID" value="Nwi_2937"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2937"/>
    <dbReference type="HOGENOM" id="CLU_2586158_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="80" mass="9484" checksum="D226273DC7424424" modified="2005-10-11" version="1">MVKERRYKNGSHARRCSFRYRSHRRDFGDRDHRDRGTIEELTKAWAWVNNNEALINEGRALPSGRTAELVELLTPQDDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNI0</accession>
    <name>Q3SNI0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Heavy metal efflux pump CzcA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2911</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316091.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2911"/>
    <dbReference type="EnsemblBacteria" id="ABA06161">
        <property type="protein sequence ID" value="ABA06161"/>
        <property type="gene ID" value="Nwi_2911"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2911"/>
    <dbReference type="eggNOG" id="COG3696">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002755_1_2_5"/>
    <dbReference type="OMA" id="ERKFNIM"/>
    <dbReference type="OrthoDB" id="91817at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2090.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027463">
        <property type="entry name" value="AcrB_DN_DC_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001036">
        <property type="entry name" value="Acrflvin-R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004763">
        <property type="entry name" value="CzcA/CusA/SilA/NccA/HelA/CnrA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32063">
        <property type="entry name" value="PTHR32063"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00873">
        <property type="entry name" value="ACR_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00702">
        <property type="entry name" value="ACRIFLAVINRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82714">
        <property type="entry name" value="SSF82714"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00914">
        <property type="entry name" value="2A0601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="336"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="387"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="449"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="481"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="537"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="877"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="903"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="931"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="978"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1006"/>
            <end position="1032"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA06161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1050" mass="114022" checksum="52C8016DE9D55026" modified="2005-10-11" version="1">MINPIVAFALRQRVLVLVLFMMTMAGGIAAFRVLNIEAYPDPVPPMVDVITQSAGLSAEEIERYITIPIETQLSGVPNLRVMRTISLYGLSDVKLQFTYAYTYDEALQQVLNRLSQLSGLPDGAQPTISPVSPVGEVYRYRLVGPPGFSVLDLKTLQDWLLQRRFRAVPGIVDVIGWGGKTKTFELQVDLDKLVAHRLTLTQLLQTLRSSNLNVGGNIVNLGTQSAVVRGVGLIRSSDDINNTMLTQAGGNPVLVRDVGTVIIGHQPRLGIAGQDNDDDIVQGIVLMRRGQQSSPTIERVKAEVERINTSGILPPGVRIERIYDRQDLIDTTTHTVLHNMTVGILLIFALQWVFLGNLRSALIVGATIPFALFFAIGIMVLRGESANLLSVGAIDFGLIVDATVIMVERIFRELGHGGERTFPEDDNAAGLSGKLLAIYAAGAGVNRSILFATGIIIAGFIPLFTMSGVEGHIFGPMAKTYAYAIAGGLLATFTVTPALSALLLPEHVQETETWLVRTLHRFYSPVLRIAVANKRSTVAVTLAVFVLAGIAMRFLGLEFLPKLEEGNLWIRATMPATISLQEGNGYANRMRRLIRSFPEVDTVVTQHGRPDDGTDAAGFFNVEFFTPLKPVGQWRHGVDKETLTEEIQARLKMEFPGIQFNFSQYLQDNVAEQVSGVKGENSIKVFGNDLQELTDTAEEIKAVLEKVPGVTDLGVFTSLGQPTIQISIDRERAARYGLSPGDINSMIRTAVGGEAAGDLYEHGSDRHFPIVVRLAPQYRQSAEAISKLTIGVSGAKGITQIPLSEVAQIRLVTGPSYIYREQQQRYLPIKFSVRDRDLGSTIKEAEQRIAQDVKLPAGTQLQWVGEFRNLQEAIGRLQIVVPFSLGIIGLLLWINFTSIRDTLLALSVIPMAVIGGVFALFVTGIPFSVSAAIGFIALFGISVMNGILILSQYNQFIESGMKRIDAIIRAGEVQMRPVLMTCVIAGIGLLPAAVSTGIGSQVQKPLAVVVVGGMMVAPVVILVALPALIALFSRRRTVVAAPEAACKPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNI5</accession>
    <name>Q3SNI5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2906</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316086.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2906"/>
    <dbReference type="EnsemblBacteria" id="ABA06156">
        <property type="protein sequence ID" value="ABA06156"/>
        <property type="gene ID" value="Nwi_2906"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2906"/>
    <dbReference type="eggNOG" id="COG3683">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088941_0_1_5"/>
    <dbReference type="OMA" id="DEMFGAY"/>
    <dbReference type="OrthoDB" id="1698942at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010412">
        <property type="entry name" value="DUF1007"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016537">
        <property type="entry name" value="UCP008159_ABC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06226">
        <property type="entry name" value="DUF1007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008159">
        <property type="entry name" value="UCP008159_ABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229173" evidence="1">
        <location>
            <begin position="25"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="214" mass="23760" checksum="1DA61ECFF242788D" modified="2005-10-11" version="1">MSRIRSIICLALLFLAGTLMSASAHPHVWVTIRSEILYTPDGVMSGIRHAWTFDDMFSAYALQGIHHATKGQYTREELNALAEVNVTSLKENDYFTYAKADGKKATFSDPVDYWLEYKDSALILHFTLPLKTPVAAKAMLIEVYDPTIFVDFGLAKDQPVALAGAPASCQLIVDSPRTPTADEQLRMSQLDQNPLDSLDTFGQGFSNKIRIKCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNK4</accession>
    <name>Q3SNK4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribonucleoside-diphosphate reductase subunit beta</fullName>
            <ecNumber evidence="3">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2887</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>[thioredoxin]-disulfide + a 2'-deoxyribonucleoside 5'-diphosphate + H2O = [thioredoxin]-dithiol + a ribonucleoside 5'-diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23252"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="EC" id="1.17.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3 4">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="3 4">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 3">Genetic information processing; DNA replication.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the ribonucleoside diphosphate reductase small chain family.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2887"/>
    <dbReference type="EnsemblBacteria" id="ABA06137">
        <property type="protein sequence ID" value="ABA06137"/>
        <property type="gene ID" value="Nwi_2887"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2887"/>
    <dbReference type="eggNOG" id="COG0208">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035339_1_1_5"/>
    <dbReference type="OMA" id="KVGEYQR"/>
    <dbReference type="UniPathway" id="UPA00326"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01049">
        <property type="entry name" value="RNRR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.620.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012348">
        <property type="entry name" value="RNR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033909">
        <property type="entry name" value="RNR_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000358">
        <property type="entry name" value="RNR_small_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409">
        <property type="entry name" value="PTHR23409"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00268">
        <property type="entry name" value="Ribonuc_red_sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000355">
        <property type="entry name" value="NrdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="24"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR000355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000355-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA06137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="391" mass="44597" checksum="AF26CF752AF61A3E" modified="2005-10-11" version="1">MLDWSDTSPSTPVRLVPPQALAHNDVASDSSPRPTKTDAPTDAAGLGDIDRGGARVRVDDKRMINARADVNQLLPLKYRWAWEKYLSGCNNHWMPTEVSMQADIALWKSKDGLTDDERRMVKRNLGFFAASESLVANNIVLAIYRHLTNPECRQYLLRQAFEEAVHTHTFQYIVESLGLDEGELFNMYREVPSITDKAAWALKHTRHLDDPGFKTGTPEADQAFLRDLVAFYVVFEGMWFYTGFVQILSLGRRNKMVGIAEQYQYILRDESIHLNFGIDIINQIKIENPHLWSKPFQEEVRAMLKNAADLEAAYGRDTMPRGFLGLNATLCETYMHVIANRRCAQLGLAPVFPDADNPFPWMSEAMDLKKEKNFFETRVIEYQNGGALAWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNL0</accession>
    <name>Q3SNL0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">TonB-dependent siderophore receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_2881</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the TonB-dependent receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316061.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2881"/>
    <dbReference type="EnsemblBacteria" id="ABA06131">
        <property type="protein sequence ID" value="ABA06131"/>
        <property type="gene ID" value="Nwi_2881"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2881"/>
    <dbReference type="eggNOG" id="COG4773">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008287_9_0_5"/>
    <dbReference type="OMA" id="FSWRPDD"/>
    <dbReference type="OrthoDB" id="173242at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015344">
        <property type="term" value="F:siderophore uptake transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012910">
        <property type="entry name" value="Plug_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037066">
        <property type="entry name" value="Plug_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011662">
        <property type="entry name" value="Secretin/TonB_short_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039423">
        <property type="entry name" value="TonB-dep_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000531">
        <property type="entry name" value="TonB-dep_rcpt_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036942">
        <property type="entry name" value="TonB_rcpt_b-brl_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010105">
        <property type="entry name" value="TonB_sidphr_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32552">
        <property type="entry name" value="PTHR32552"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07715">
        <property type="entry name" value="Plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07660">
        <property type="entry name" value="STN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00593">
        <property type="entry name" value="TonB_dep_Rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00965">
        <property type="entry name" value="STN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01783">
        <property type="entry name" value="TonB-siderophor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="4 8" id="KW-0798">TonB box</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="STN" evidence="9">
        <location>
            <begin position="84"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA06131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="821" mass="89156" checksum="A0E07EDE2BD3D847" modified="2005-10-11" version="1">MAFRLEAVKNKQGTPFTAGSLRRITSWLLASTILCCAGVGLSSPSLAQQPAPAPASAQSRAVQFSIPAQPLPAALDAFIRASGWQVGYSTRLTDGHRSTAVNGAMPPAQALRMLLAGTGINVSLTGANTATLVGPNSAAAGGAAPAGAIALDTIDIQGETAWGPVNGFVASRSATATKTDTPILEVPQSISVVTRDQIETRRNQTLNETLQYTPGVLAERTGQQQFAPRFQVRGFSADGFNGAIYLNGLRGATNWSDIEPYGLERIEVMRGPASVLYGQGQPSGVIALVTKRPSEKPIHEFQLQGGSFEQRSGAFDFGGPVTEDGKLLYRFTGLLREGGNGIDFSNDKRAFLSGALTWRPTAETEITAYSLHQRNQGRWNFGLPARGTVLPNPNGRIPVTRFIGEPDTYNRTEMTVAGYNLQHHVTDSLTFRQNVQYSRESWERRDVNPTSVGADLRTVNRRAESRHVTRDTFAIDNHAELKAMTGPLQHTVLFGVDHRRGKTAVLGNYGTVGPIDVFAPVYGSPVVWSTTDQYAERQPENFIGVYVQDQIKLDQWILTLGGRHDWADTSIVDIIGGSVTDQNNRAFTKRAGLGYEFDSGVVPYVSYAESFTPLSGRTFDGSPLRPETGVQYEAGIKYQPRDMNLRITAAVYDLQRQNVTTADLANPGFSIQIGEVTSRGFEFEAVASLTSNLNLTAAYAYNDARVTQSNDANLGKRPVTTPPHLASLWADYTIREGALNGLGFGGGIRYVSASAGDFLNTFEAPAYTVVDAMARYDIDNWRFSLNVSNLFDNQYVVGCFSLVQCNVGRVRTVLGRVSYRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNM0</accession>
    <name>Q3SNM0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2871</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316053.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2871"/>
    <dbReference type="EnsemblBacteria" id="ABA06121">
        <property type="protein sequence ID" value="ABA06121"/>
        <property type="gene ID" value="Nwi_2871"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2871"/>
    <dbReference type="eggNOG" id="COG2333">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044335_0_0_5"/>
    <dbReference type="OrthoDB" id="1016599at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="292" mass="32647" checksum="0DCCE1854D524495" modified="2005-10-11" version="1">MWSSPVVFRRAGANGESLCEDAKAWQKEARRRVALFRENGFSTEEGDRILIMGEDIDGKTDDILDIVIKRDGTIDACNRVQDSSFQGRLLAPLPPVDDDDDETLRHNQSSVIIRFSLRGGGISDRCRFLTGGDAEVAIWNRLWNLHKAERADWFTYDIMETPHHCSWRSLSFDRWSEMGEDVKVDPDARSALSQTRKGAIIVASCKPIKAADDNPPHERAKREYIDILDDDADRFFCTDEYWNDKKVALEFEVKSNGIVRKIGKASALAAPALGISATASQARPHGTGHGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNS5</accession>
    <name>Q3SNS5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PepSY-associated TM helix</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2813</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316011.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2813"/>
    <dbReference type="EnsemblBacteria" id="ABA06066">
        <property type="protein sequence ID" value="ABA06066"/>
        <property type="gene ID" value="Nwi_2813"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2813"/>
    <dbReference type="eggNOG" id="COG3295">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114004_0_0_5"/>
    <dbReference type="OMA" id="GFTLNHA"/>
    <dbReference type="OrthoDB" id="1921658at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032307">
        <property type="entry name" value="PepSY_TM-like_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40115">
        <property type="entry name" value="PTHR40115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16357">
        <property type="entry name" value="PepSY_TM_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="221" mass="24572" checksum="69C46618A59E5060" modified="2005-10-11" version="1">MDNPDRLTVDGDELWQRKKRRAFFINQLYQWHWISSGLCLIGMLLFALTGITLNNAGQIESKPAVTMRQAQLPQALIADLTSDPGNAKAALPSSINEWLRREIGVSTAERDTEWSSDEVYVALPRPGGDAWLSIQRDGSGAVSYEVTDRGWIAYLNDLHKGRHTGKAWSWFIDVFAAAAITFCLTGLLLLQLHAKRRPATWPVVTLGLIIPTLLAVLLIHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNV1</accession>
    <name>Q3SNV1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2787</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315985.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06040">
        <property type="protein sequence ID" value="ABA06040"/>
        <property type="gene ID" value="Nwi_2787"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2787"/>
    <dbReference type="eggNOG" id="ENOG5030N94">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_176304_1_0_5"/>
    <dbReference type="OMA" id="HWYRPDP"/>
    <dbReference type="OrthoDB" id="2101210at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229000" evidence="1">
        <location>
            <begin position="28"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="101" mass="11073" checksum="68FDC69AF5FB7063" modified="2005-10-11" version="1">MKRYLGVSLAAAIIAFCGVAFIESAKAVPGAVTVSAQPGTLIEVGATRRPHRASRGYRHQRARVYVRGPYQPYYGRPSYYAPGKVTPFFPFGFGYGLDPSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNZ9</accession>
    <name>Q3SNZ9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sel1-like repeat</fullName>
            <ecNumber evidence="3">3.5.2.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2739</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.2.6" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315937.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2739"/>
    <dbReference type="EnsemblBacteria" id="ABA05992">
        <property type="protein sequence ID" value="ABA05992"/>
        <property type="gene ID" value="Nwi_2739"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2739"/>
    <dbReference type="eggNOG" id="COG0790">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_36_1_5"/>
    <dbReference type="OMA" id="YYRRGCH"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008800">
        <property type="term" value="F:beta-lactamase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229075" evidence="2">
        <location>
            <begin position="22"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="346" mass="36479" checksum="D305A25460DD3F0B" modified="2005-10-11" version="1">MSLLRPVAIAAGLLMLGESAAAQLQPSPSADAPSAGKSVKTKAIKPPPPTPPAGLEPDNKANAQVADDPNADPVYGAYQRGLYKTAFDLALKRAQEDKNPAAMTMLGELYANGLGVRRDYGKAIEWHQRAADLGDREAMFALAMLRISGRGGPPDRTGAVKWLAASAKLGQPKAAYNLALLHMDGQTLPQDFKRAAELLRFAADAGSPEAQYALATFYKEGTGVEKNLYKSVRLLQAASLAGNVDAEVEYAIALFNGSGTGKNEAAAVSLLRKAARRNSAIAQNRLAHALVEGMGVPMDKVEGLKWHIVAKTGGKGDLKLDAAMAQATPEERAGAESAARKWLGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP36</accession>
    <name>Q3SP36_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2702</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315901.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2702"/>
    <dbReference type="EnsemblBacteria" id="ABA05955">
        <property type="protein sequence ID" value="ABA05955"/>
        <property type="gene ID" value="Nwi_2702"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2702"/>
    <dbReference type="eggNOG" id="ENOG50331ZS">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_175521_0_0_5"/>
    <dbReference type="OMA" id="WAEARHD"/>
    <dbReference type="OrthoDB" id="1988299at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="88" mass="9438" checksum="2F01DEB645A27092" modified="2005-10-11" version="1">MHPPFAFSPARDSFADQEQKQAALSYLNEAWADARHDGVDGDCLAQASLFAAFAELVSTYGEDAVATFVEGLAGRVRNGEFSIALAKQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-03-03" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP44</accession>
    <name>F16PA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fructose-1,6-bisphosphatase class 1</fullName>
            <shortName evidence="1">FBPase class 1</shortName>
            <ecNumber evidence="1">3.1.3.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">D-fructose-1,6-bisphosphate 1-phosphohydrolase class 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fbp</name>
        <name type="ordered locus">Nwi_2694</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="EC" id="3.1.3.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FBPase class 1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.11" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315893.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SP44"/>
    <dbReference type="SMR" id="Q3SP44"/>
    <dbReference type="STRING" id="323098.Nwi_2694"/>
    <dbReference type="EnsemblBacteria" id="ABA05947">
        <property type="protein sequence ID" value="ABA05947"/>
        <property type="gene ID" value="Nwi_2694"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2694"/>
    <dbReference type="eggNOG" id="COG0158">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039977_0_0_5"/>
    <dbReference type="OMA" id="HEKSECY"/>
    <dbReference type="OrthoDB" id="945770at2"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042132">
        <property type="term" value="F:fructose 1,6-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00354">
        <property type="entry name" value="FBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01855">
        <property type="entry name" value="FBPase_class1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044015">
        <property type="entry name" value="FBPase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000146">
        <property type="entry name" value="FBPase_class-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033391">
        <property type="entry name" value="FBPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028343">
        <property type="entry name" value="FBPtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020548">
        <property type="entry name" value="Fructose_bisphosphatase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11556">
        <property type="entry name" value="PTHR11556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00316">
        <property type="entry name" value="FBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18913">
        <property type="entry name" value="FBPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500210">
        <property type="entry name" value="FBPtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000904">
        <property type="entry name" value="FBPtase_SBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00115">
        <property type="entry name" value="F16BPHPHTASE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00124">
        <property type="entry name" value="FBPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000364614" description="Fructose-1,6-bisphosphatase class 1">
        <location>
            <begin position="1"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="112"/>
            <end position="115"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01855"/>
        </source>
    </evidence>
    <sequence length="345" mass="36840" checksum="C50E1F7B9770EAB6" modified="2005-10-11" version="1">MSQEMTLQERMDGEGGSDPLRQAVKGAVAALAQAAIDISDLTCRGALAGITGQAQGRNTDGDVQKDLDVRADQIIRDALGKQPIAALASEEMEELDILNPGAPICVAFDPLDGSSNINTNMSVGTIFSIMPTPSDVNAAFRQAGSEQLAAGFVVYGPQTSLVLTLGQGVDIFTLDRVDRVFKLTGSAVKIPAEATEFAINASNRRHWDLPVRAYIDECLAGADGAGGKNYNMRWIGSLVAEAFRILIRGGIFLYPGDARDGYEEGRLRLVYEAHPMAFIVEQAGGGASTGRKRVLDLVPGSLHQRVPLIMGSIKNVQRLEHLHTVPDGALEANAPLFGNRGLFRV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-06-12" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP57</accession>
    <name>NDVA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Beta-(1--&gt;2)glucan export ATP-binding/permease protein NdvA</fullName>
            <ecNumber evidence="2">7.5.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ndvA</name>
        <name type="ordered locus">Nwi_2681</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in beta-(1--&gt;2)glucan export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>[(1-&gt;2)-beta-D-glucosyl](n)(in) + ATP + H2O = [(1-&gt;2)-beta-D-glucosyl](n)(out) + ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:18453"/>
            <dbReference type="Rhea" id="RHEA-COMP:11881"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:27517"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.5.2.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text>In NdvA the ATP-binding domain (NBD) and the transmembrane domain (TMD) are fused.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. Beta-(1--&gt;2)glucan exporter (TC 3.A.1.108.1) family.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="ABA05934" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="7.5.2.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05934.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SP57"/>
    <dbReference type="SMR" id="Q3SP57"/>
    <dbReference type="STRING" id="323098.Nwi_2681"/>
    <dbReference type="EnsemblBacteria" id="ABA05934">
        <property type="protein sequence ID" value="ABA05934"/>
        <property type="gene ID" value="Nwi_2681"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2681"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_3_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015441">
        <property type="term" value="F:ABC-type beta-glucan transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005896">
        <property type="entry name" value="NdvA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01192">
        <property type="entry name" value="chvA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51317">
        <property type="entry name" value="NDVA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0762">Sugar transport</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000290249" description="Beta-(1--&gt;2)glucan export ATP-binding/permease protein NdvA">
        <location>
            <begin position="1"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="2">
        <location>
            <begin position="21"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="2">
        <location>
            <begin position="345"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="378"/>
            <end position="385"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="609" mass="66644" checksum="3DFA6A6758CB2FA7" modified="2007-06-12" version="2">MSMVRLYTRVLELLGAEARLGWILAGANLLLAGAQFAEPVLFGRIIDVLSGNPASGLFGMASTSPWPLLAVWAAFGLFTILCGVTVALQADRLSHRQRQAVLTGYFEHIMQLPLAYHAGTHSGRLMKVMLQGTDALWRLWLGFFREHFAAMMSLVVLLPLSLYINWRLAILLFALCIVFTMLTTLVVRRTFDMQNEVEAHFSDLSARASDALGNVALVQSFVRVDAEVQGLRFVVDKLLSAQMPVLSWWAVVTVMTRASTTITILAIFTAGIALNQRGMTSIGEIVMFVSFATMLIQRLEQVVSFINSVFMEAPRLKEFFDVLDAVPAVRDRPDAVDPGRLQGRVEFNDVSFSYDSKRPAVANLSFVASPGETIALVGPTGAGKSTAVALLHRAFDPQSGIIRIDGMDIRDLTLSGLRRNIGVVFQEPLLFNRSIAENLRVGKPDATDEEMRLAAGRAQALDFIERGEKKFDTHAGERGRMLSGGERQRLSIARALLKDPPILILDEATSALDAVTEAKVNAALDEVMKGRTTFVIAHRLSTIRNAARILVFADGRVIESGTFGELLANGGHFAQLAKAQFMTQESTQADLSSSRTNPAVEIRPLSLGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP66</accession>
    <name>Q3SP66_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2672</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05925">
        <property type="protein sequence ID" value="ABA05925"/>
        <property type="gene ID" value="Nwi_2672"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2672"/>
    <dbReference type="HOGENOM" id="CLU_2155692_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="111" mass="12456" checksum="62CDCF9272326774" modified="2005-10-11" version="1">MTPPARFTRAGTSLSGRQVESSESLSFLIQSEARLSGLDVMRFLHANSSSHRLAANRARAPETTAIEVWNVCKPTLGTEARPWRSMPFIIRRYRWLTLATPIAVMTAVPDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPA2</accession>
    <name>Q3SPA2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2636</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315839.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05889">
        <property type="protein sequence ID" value="ABA05889"/>
        <property type="gene ID" value="Nwi_2636"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2636"/>
    <dbReference type="HOGENOM" id="CLU_1609093_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011048">
        <property type="entry name" value="Haem_d1_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51004">
        <property type="entry name" value="SSF51004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="165" mass="17618" checksum="AE7B679A461342EE" modified="2005-10-11" version="1">MIAAGDTAQRRLAAGSLNGPVAAGDGVIASAGPRQDVLLRWLDDLDRAVPVPVGIAIDGLFVRDDGRYLVAWSAREPEAVIVDVARSRVAGGVRTDDIVDEAASAGSAIFLTHRTSPTVIAIDLAPLSSREATFAERRVRLPLIAGANREHSRSRVAYRRRWPAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPB5</accession>
    <name>Q3SPB5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2623</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315826.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2623"/>
    <dbReference type="EnsemblBacteria" id="ABA05876">
        <property type="protein sequence ID" value="ABA05876"/>
        <property type="gene ID" value="Nwi_2623"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2623"/>
    <dbReference type="eggNOG" id="ENOG5032ZH4">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_182933_0_0_5"/>
    <dbReference type="OMA" id="THRSSWP"/>
    <dbReference type="OrthoDB" id="2054258at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046093">
        <property type="entry name" value="DUF6111"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19606">
        <property type="entry name" value="DUF6111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="87" mass="9465" checksum="5BB2BD60C71F4976" modified="2005-10-11" version="1">MMRPILMELGIFLIPFAVYAAYLIANRVDFMIAASWPAHVVLRLSLAALVLVVVSFILLANFSGAPPDSTYIPAHIENGKLVPGAER</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPB9</accession>
    <name>Q3SPB9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">7-carboxy-7-deazaguanine synthase</fullName>
            <shortName evidence="5">CDG synthase</shortName>
            <ecNumber evidence="5">4.3.99.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Queuosine biosynthesis protein QueE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">queE</name>
        <name evidence="7" type="ordered locus">Nwi_2619</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>6-carboxy-5,6,7,8-tetrahydropterin + H(+) = 7-carboxy-7-deazaguanine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:27974"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:61032"/>
            <dbReference type="ChEBI" id="CHEBI:61036"/>
            <dbReference type="EC" id="4.3.99.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </cofactor>
        <text evidence="5">Binds 1 S-adenosyl-L-methionine per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="5">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the radical SAM superfamily. 7-carboxy-7-deazaguanine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.3.99.3" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315822.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2619"/>
    <dbReference type="EnsemblBacteria" id="ABA05872">
        <property type="protein sequence ID" value="ABA05872"/>
        <property type="gene ID" value="Nwi_2619"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2619"/>
    <dbReference type="eggNOG" id="COG0602">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066739_0_1_5"/>
    <dbReference type="OMA" id="CDVKESW"/>
    <dbReference type="OrthoDB" id="1141206at2"/>
    <dbReference type="UniPathway" id="UPA00391"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016840">
        <property type="term" value="F:carbon-nitrogen lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008616">
        <property type="term" value="P:queuosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="QueE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="7-CO-7-deazaguanine_synth-like"/>
    </dbReference>
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        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030977">
        <property type="entry name" value="QueE_Cx14CxxC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000370">
        <property type="entry name" value="QueE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00376">
        <property type="entry name" value="7-carboxy-7-deazaguanine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04508">
        <property type="entry name" value="queE_Cx14CxxC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3 5" id="KW-0408">Iron</keyword>
    <keyword evidence="4 5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0671">Queuosine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="6">
        <location>
            <begin position="18"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="12"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="5">
        <location>
            <begin position="48"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="5">
        <location>
            <begin position="133"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="5">
        <location>
            <begin position="173"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="210" mass="22798" checksum="D50382A391919776" modified="2005-10-11" version="1">MSYAVKEIFLTLQGEGAHAGRAAVFCRFSGCNLWSGREEDRASATCRFCDTDFVGTDGTLGGRYASADALADTVAGQWAGPAAHRYAVLTGGEPLLQVDKAFVDALHARGFEIGIETNGTLAPPDGIDWLCVSPKAGADLRIRRGHELKLVYPQADARPEDFADLAFDRFSLQPMDGPDAAENTARAVDYCLRHPQWRLSLQTHKTLGIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPE4</accession>
    <name>Q3SPE4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">TPR repeat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2594</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2594"/>
    <dbReference type="EnsemblBacteria" id="ABA05847">
        <property type="protein sequence ID" value="ABA05847"/>
        <property type="gene ID" value="Nwi_2594"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2594"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
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    <dbReference type="OMA" id="YLGYSWI"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041656">
        <property type="entry name" value="TPR_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12688">
        <property type="entry name" value="TPR_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
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        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229023" evidence="3">
        <location>
            <begin position="51"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="305"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="TPR_5" evidence="4">
        <location>
            <begin position="341"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="515"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="583"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="617" mass="68236" checksum="C14F4AA308DB693A" modified="2005-10-11" version="1">MVPARVAYRETLMFVPRNLELRIPMLSIRFNRLAMAVAAAALLVPSGPLAAQTPDHPTETSPFPSAKDLRSMTTSGSYLAARHASVERDARSAATFYRSALRTDPKNNELLDRAFISSLAEGNIAAAVKLADRILAIDKSNRVARLVVGVDNLKQKKYAAARKNIKLSIRGPITDLVATLLSGWADYGAGDTRQAVALIDALTGPEWYPIFKDLHSGMLLDLAGRKEEAGARLERAYKLDDSALRVSDAYARWLSRNKDAAAALAVYESFDKKLARHPLIQEGIKELKARKKLPQLVNSPQTGAAEALYGIGASLTRRGGEDLALVYLQLALYLSPDHPMALLSLADLYESVKKPEMAIGVYERVPAGSPLRRNAQIQLATNLDAVDRSDEAIKILKGVTEQDPNDLEAIMALGNVERGRKKFADCAKTYGKGIDVIAEAKDKPNWVYYYFRGICLERSKNWAKAEVDMKKALEIQPEQPHVLNYLGYSWIDRGLNLDEAMKMIKRAVDQRPDDGYIVDSLGWAYYRIGNYEEAVKTLERAINLKPEDPTVNDHLGDAYWRVGRTLEAKFQWAHARDLKPEQEELPKIEAKIKNGLPADDTPSAASADKKKDAGKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPP5</accession>
    <name>Q3SPP5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2493</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315699.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2493"/>
    <dbReference type="EnsemblBacteria" id="ABA05746">
        <property type="protein sequence ID" value="ABA05746"/>
        <property type="gene ID" value="Nwi_2493"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2493"/>
    <dbReference type="eggNOG" id="COG3706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1029859_0_0_5"/>
    <dbReference type="OMA" id="LMYHGRD"/>
    <dbReference type="OrthoDB" id="1116093at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="270" mass="29049" checksum="FCD9514A3E64FB89" modified="2005-10-11" version="1">MRIDWRMMLGPALAMLIAGASIYADHELAAVPNLSALYICVVALAGSLGGTASGLISAGIAVLASAGFLGDDNAAVAEGSTLLHLGLLAPAAGGAALITGLLRSRMMNALERERERHATAARLTAALDQTGIGIVMLDADTRAEFINRAFRHYFSLPDEKADSRPPFIALMYHGRDTGAYELPQDELSHFIAERVRMVRAGDPTPINIKLRNGQVLRFICTALPDGGRMLSYTPVTDLIRRTDDPADADYYLSLRGGDRRLPVHRLRAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPP8</accession>
    <name>Q3SPP8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Rhodanese-related sulfurtransferase</fullName>
            <ecNumber evidence="3">2.8.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2490</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.8.1.2" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2490"/>
    <dbReference type="EnsemblBacteria" id="ABA05743">
        <property type="protein sequence ID" value="ABA05743"/>
        <property type="gene ID" value="Nwi_2490"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2490"/>
    <dbReference type="eggNOG" id="COG2897">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031618_3_0_5"/>
    <dbReference type="OMA" id="LLDVRWQ"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016784">
        <property type="term" value="F:3-mercaptopyruvate sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001307">
        <property type="entry name" value="Thiosulphate_STrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045078">
        <property type="entry name" value="TST/MPST-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11364">
        <property type="entry name" value="PTHR11364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="SSF52821"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00380">
        <property type="entry name" value="RHODANESE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="48"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="173"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="292" mass="31028" checksum="D82D82496DC06982" modified="2005-10-11" version="1">MSLMMDIAMNPTLDDPLVSTEWLAAHLGEVKAIDASFKMPGVLPLAVDDFYAAHIPGAVFFDVDAVSDRASPLPHMYPDAAQFGRDVGALGISSKDTVVVYDNGGWLAGPRAWWMFLSFGHAGVRVLDGGLKKWRAEGRPVESGKVSPEPGHFTATFDPLFVRDKAQLVSNLSSCREQLVDARAAARFTGAVMEPRQGLRSGHIPGSRNLSYAELFDPGTGVMKPLDDIRAAFSRAGVDLAKPVVTTCGSGVSAAVLTLALYRLGARGSALYDGSWSEWGLVEGPPIATGPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="133" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPT9</accession>
    <name>Q3SPT9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Carbamoyl-phosphate synthase large chain</fullName>
            <ecNumber evidence="13">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Carbamoyl-phosphate synthetase ammonia chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">carB</name>
        <name evidence="17" type="ordered locus">Nwi_2449</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="13">Binds 4 Mg(2+) or Mn(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="13">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the CarB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.5" evidence="13"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315657.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2449"/>
    <dbReference type="EnsemblBacteria" id="ABA05702">
        <property type="protein sequence ID" value="ABA05702"/>
        <property type="gene ID" value="Nwi_2449"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2449"/>
    <dbReference type="eggNOG" id="COG0458">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000513_1_0_5"/>
    <dbReference type="OMA" id="IEPAGIH"/>
    <dbReference type="OrthoDB" id="48855at2"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00171"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01424">
        <property type="entry name" value="MGS_CPS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1030.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01210_B">
        <property type="entry name" value="CPSase_L_chain_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006275">
        <property type="entry name" value="CarbamoylP_synth_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005480">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036897">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CbamoylP_synth_lsu-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005483">
        <property type="entry name" value="CbamoylP_synth_lsu_CPSase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033937">
        <property type="entry name" value="MGS_CPS_CarB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02787">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00098">
        <property type="entry name" value="CPSASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01096">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48108">
        <property type="entry name" value="SSF48108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="SSF52335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01369">
        <property type="entry name" value="CPSaseII_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="13" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="9 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0464">Manganese</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 13" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ATP-grasp" evidence="15">
        <location>
            <begin position="180"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="15">
        <location>
            <begin position="739"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="domain" description="MGS-like" evidence="16">
        <location>
            <begin position="1017"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Carboxyphosphate synthetic domain" evidence="13">
        <location>
            <begin position="1"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Allosteric domain" evidence="13">
        <location>
            <begin position="1017"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="349"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="351"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="910"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="922"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="924"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>4</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ABA05702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1154" mass="124959" checksum="3A53500FBB2712DE" modified="2005-10-11" version="1">MPKRTDISTILIIGAGPIVIGQACEFDYSGTQAVKALKEEGYRVVLVNSNPATIMTDPDLADATYIEPITPEIVGKIIEKERHVVPGGFALLPTMGGQTALNCALSLRRQGTLEDFDVEMIGATADAIDKAEDRQLFREAMTRIGLETPKSRLANASAMKKADRDAYLAEKEKLSGDALAELERQWTLGEGERRKRYQQQAMAEALMALSEIGLPAIIRPSFTMGGTGGGIAYTRDEFLDIIERGLDASPTNEVLIEESVLGWKEYEMEVVRDKKDNCIIICSIENLDPMGVHTGDSITVAPALTLTDKEYQVMRDASLAVLREIGVETGGSNVQFGVDPVSGRMVIIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDEIANDITGGATPASFEPSIDYVVTKIPRFAFEKFPGASTTLTTSMKSVGEVMAIGRTFQESLQKALRGLEIGLTGLDEIDIDGMGRGDDKNAIRAALGTPTPSRLLQLAQAMRLGWTDEEIFSSCKIDPWFLAELRGIVEMENKVRSHGLPGNAFGMRTLKAMGFSDARLAVLSGRTEAEVKALRRTLGVRPVYKRIDTCAAEFASPTAYMYSTYEAPFAGEAADESAPSDKTKVVILGGGPNRIGQGIEFDYCCCHACFALSDAGFETIMVNCNPETVSTDYDTADRLYFEPLTAEDVLEIIDTERQNGTLHGVIVQFGGQTPLKLAHALEAADVPILGTSPEAIDLAEDRDRFKRILDRLKLKQPKNGIAYSVEQARLVAADLGLPLVVRPSYVLGGRAMQIIREESQLGDYLLETLPELVPADVKARYPNDKTGQINTVLGTNPLLFDRYLSDATEIDVDCLGDGKDTYVVGIMEHIEEAGIHSGDSACSLPPRSLDAATIAELERQTRELALGLDVVGLMNVQYAIKDGEIYVLEVNPRASRTVPFVAKVIGLPVAKIAARIMAGEKLAGFELKPQRLDHVGVKESVFPFARFPGVDTVLGPEMKSTGEVMGIDRSFEIAFAKSQLGGGTHLPRKGTVFVSVRETDKMRILATVKLLASVGFRVIATSGTQRYLTDSGVSAEKINKVLEGRPHIVDAITNGEVQLVFNTTDGPQALADSRSLRRAALLHKVPYYTTLSGAVAAAQGIRAYLGGDLEVRTLQSYFSET</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPU1</accession>
    <name>Q3SPU1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ferritin and Dps</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2447</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the Dps family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315655.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2447"/>
    <dbReference type="EnsemblBacteria" id="ABA05700">
        <property type="protein sequence ID" value="ABA05700"/>
        <property type="gene ID" value="Nwi_2447"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2447"/>
    <dbReference type="eggNOG" id="COG0783">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098183_2_0_5"/>
    <dbReference type="OMA" id="AHWNLVG"/>
    <dbReference type="OrthoDB" id="1742631at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01043">
        <property type="entry name" value="DPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002177">
        <property type="entry name" value="DPS_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008331">
        <property type="entry name" value="Ferritin_DPS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42932">
        <property type="entry name" value="PTHR42932"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00210">
        <property type="entry name" value="Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005900">
        <property type="entry name" value="Dps"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01346">
        <property type="entry name" value="HELNAPAPROT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin" evidence="4">
        <location>
            <begin position="33"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="178" mass="20308" checksum="4CD0462E360901F8" modified="2005-10-11" version="1">MTQAKSSALRKVNPELDTPTDLSEEGVSKISTAMNTILADTFALYLKTKNFHWHVSGRHFRDYHLLLDEQSEQIFGTTDEIAERVRKIGGTTLRSIGQIAKLQTLEDNDESFVPPREMLRELMNDNKKVAAAMRKAHDICDKYEDVATASLIENFIDATERRVWFLFEASRQEGDNEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPY2</accession>
    <name>Q3SPY2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DegT/DnrJ/EryC1/StrS aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2405</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the DegT/DnrJ/EryC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315620.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2405"/>
    <dbReference type="EnsemblBacteria" id="ABA05659">
        <property type="protein sequence ID" value="ABA05659"/>
        <property type="gene ID" value="Nwi_2405"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2405"/>
    <dbReference type="eggNOG" id="COG0399">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033332_2_1_5"/>
    <dbReference type="OMA" id="RFWATQA"/>
    <dbReference type="OrthoDB" id="1722208at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00616">
        <property type="entry name" value="AHBA_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026385">
        <property type="entry name" value="AminoTrfase_NHT_00031"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000653">
        <property type="entry name" value="DegT/StrS_aminotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30244">
        <property type="entry name" value="PTHR30244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01041">
        <property type="entry name" value="DegT_DnrJ_EryC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000390">
        <property type="entry name" value="PLP_StrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04181">
        <property type="entry name" value="NHT_00031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="229"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR000390-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000390-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="402" mass="43678" checksum="A7ACE93C024C2301" modified="2005-10-11" version="1">MAISAQAGPAVEACFLSSILEAVDLALSSAKRPVALHEPRFGARERELVMDCVDTGWVSSAGRYVTLFEEMVASAVGVPHAVAVVNGTTALHAALVLEGVRPGDEVIVPSITFVATANAVSHAGAVPHFVDSTWMTMGLDPVALDEHLQQVSERRDGQLFNRQTGRRIRAVVPVHVFGHPVDVPQLQALLAKYDLIFIEDATESLGSTWKGQACGTFGHSAVLSFNGNKIITTGGGGMILTADDQYARRARHLTTTAKIAHQWAFEHDEVGYNYRLPNINAALGCGQVERLPEMVAAKRALATRYMQVFENVSGARIFREPGGAASNYWLNTLVLDREFSDERDALLSMLHANGIQARPLWTPLHLLPMYSDCPRAPLPVAEDMFTRCINLPSSPFLSGERN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ21</accession>
    <name>Q3SQ21_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2366</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315582.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2366"/>
    <dbReference type="EnsemblBacteria" id="ABA05620">
        <property type="protein sequence ID" value="ABA05620"/>
        <property type="gene ID" value="Nwi_2366"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2366"/>
    <dbReference type="HOGENOM" id="CLU_2451633_0_0_5"/>
    <dbReference type="OMA" id="ASWSLWA"/>
    <dbReference type="OrthoDB" id="2149849at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="89" mass="9804" checksum="51E6AB79AB806E20" modified="2005-10-11" version="1">MLQNLGRVFLAIVIPIYLGYVRASLNVFDVYLILAASWSLWAYGQPGPRELWESGGIIAVLKGWLVMVFAILLVAGPAYGVGYLIAKYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ25</accession>
    <name>Q3SQ25_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transport permease protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_2362</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell inner membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ABC-2 integral membrane protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315578.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2362"/>
    <dbReference type="EnsemblBacteria" id="ABA05616">
        <property type="protein sequence ID" value="ABA05616"/>
        <property type="gene ID" value="Nwi_2362"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2362"/>
    <dbReference type="eggNOG" id="COG0842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039483_3_0_5"/>
    <dbReference type="OMA" id="AWIFKTG"/>
    <dbReference type="OrthoDB" id="1891092at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC_2_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006648">
        <property type="entry name" value="DrrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00164">
        <property type="entry name" value="ABC2TRNSPORT"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="55"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="198"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="221"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="ABC2_membrane" evidence="7">
        <location>
            <begin position="6"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA05616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="253" mass="28084" checksum="91E622754A2F5DD6" modified="2005-10-11" version="1">MNFGAVQAIYKFEMARTWRTLLQSIVSPVVSTSLYFVVFGAAIGSRITEVEGVSYGTFIVPGLIMLSVLTQSITNASFGIYFPKFIGTIYEILSAPVSYFEIIMGYVGAAATKSIILGLIILATAGLFVPLHIHHPIWMLTFLVLTSVTFSLFGFIIGIWADGFEKLQMIPMLIITPLTFLGGSFYSINMLPPAWQTAALFNPVVYLISGFRWSFYEIADVSLSVSIGMTVGFLVVCMLVVWWIFRTGYRLRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ60</accession>
    <name>Q3SQ60_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Monooxygenase, FAD-binding protein</fullName>
            <ecNumber evidence="2">1.14.13.20</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2327</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.14.13.20" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2327"/>
    <dbReference type="EnsemblBacteria" id="ABA05581">
        <property type="protein sequence ID" value="ABA05581"/>
        <property type="gene ID" value="Nwi_2327"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2327"/>
    <dbReference type="eggNOG" id="COG0654">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033626_0_0_5"/>
    <dbReference type="OMA" id="CRFIAFM"/>
    <dbReference type="OrthoDB" id="867226at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018666">
        <property type="term" value="F:2,4-dichlorophenol 6-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_3" evidence="1">
        <location>
            <begin position="6"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="414" mass="45796" checksum="6CF305FEFE9BAD5F" modified="2005-10-11" version="1">MTYRTCCIVGAGPAGLMLGLLLARAGIDVTVLEKHGDFLRDFRGDTVHPSTLEVMHELGLLEGLLKLPHTQAPRLHAEIDGKDITIADFSRLPTKCRFIAFMPQWDFLDYVAREAARFTNFRLIMQAEVTALIEEDGRIAGVRALTPGGDLDIRTALVVGADGRNSIVREKAGLQIERFGSQRDVLWMKLSHMPDDPPYVMGHGGPRQGFVMIDRGDYWQCGYVVRKGSFDDVKATGLDAFRAAVASISPLSPERLSEVRSWDDVHVLSIRIDRLKNWWRPGLLCIGDAAHAMSPIGGFGVNLAIQDAVAAANMLADPLRDGGLADKHLAAVEARRSFPTKATQKVQLMMQGARRKREAADSGRRDGPPSFMRRMARWPILAHLSGRLVGLGFRTEHVRERAPRVTRVRENAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ89</accession>
    <name>Q3SQ89_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Xylose isomerase-like TIM barrel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2298</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2298"/>
    <dbReference type="EnsemblBacteria" id="ABA05552">
        <property type="protein sequence ID" value="ABA05552"/>
        <property type="gene ID" value="Nwi_2298"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2298"/>
    <dbReference type="eggNOG" id="COG1082">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086649_0_0_5"/>
    <dbReference type="OMA" id="CTITFRH"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AP_endonuc_2" evidence="1">
        <location>
            <begin position="30"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="296" mass="32645" checksum="7BCCCBB1B6D2DFB6" modified="2005-10-11" version="1">MSVGGTMMKLSVCTITFRHHLISIWDIAVWARRSGFDGIELWGAHARGLARTHPHYGADWLGDLGLSVSMLSDYLPTDGDPAIVRERTQRLCRMAQHWSAPKIRTFVGDRPSVEVPEDKRKVMTSRVRDMAAIAYDHGIRLLVETHPNTLADSLASTQRLLAEVDHPGLGINFDALHVWEGGDDLVSAHRALAPYIGHYHLKNVRSRGELNVFAPGNVYAAAGCREGMVPLFEGAMSLDAFLSAALVMSDADASLEWFGEDCFETLIRDRVALLAHIGALMQRAVPREAIVEGRSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ97</accession>
    <name>Q3SQ97_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MaoC-like dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2290</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315511.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2290"/>
    <dbReference type="EnsemblBacteria" id="ABA05544">
        <property type="protein sequence ID" value="ABA05544"/>
        <property type="gene ID" value="Nwi_2290"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2290"/>
    <dbReference type="eggNOG" id="COG2030">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094876_0_1_5"/>
    <dbReference type="OMA" id="IYEHRPG"/>
    <dbReference type="OrthoDB" id="1563802at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002539">
        <property type="entry name" value="MaoC-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01575">
        <property type="entry name" value="MaoC_dehydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MaoC-like" evidence="1">
        <location>
            <begin position="10"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="150" mass="16886" checksum="EE8C2E073B1F0189" modified="2005-10-11" version="1">MGGLYFEQFSVGQTFVHEIRRTVTDMDNILFSSLTCNPAAVHIDHEYARSTEFGKPLINSIFTLGLIIGLSVQDTTFGTTVANLGMEETRFPHPVFAGDTLRAESKVISVRDSKSRPAQGIVTFEHRGYNQRDEEVVFCRRNALMMRRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQB3</accession>
    <name>Q3SQB3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2274</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315495.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05528">
        <property type="protein sequence ID" value="ABA05528"/>
        <property type="gene ID" value="Nwi_2274"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2274"/>
    <dbReference type="eggNOG" id="ENOG50316DE">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118465_0_0_5"/>
    <dbReference type="OMA" id="QTLRCAS"/>
    <dbReference type="OrthoDB" id="1707166at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229053" evidence="1">
        <location>
            <begin position="32"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="166" mass="17421" checksum="1CDDBD0134C2C380" modified="2005-10-11" version="1">MKQFAIRLIRGSAIIAGLVAGLALSAPASFAQSAPFTGFAGNWSGKGTVTLSDGSKENIRCKASYKVDGRGTAMNQSLHCASASYRFDLSSDVASHGNEITGHWSVASRNLFGSLHGRAGGGGIDVFAQAAGFAANLTIRTQGNRQSVRITSRDEIRNVSITMARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="129" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQG6</accession>
    <name>Q3SQG6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_2221</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2221"/>
    <dbReference type="EnsemblBacteria" id="ABA05475">
        <property type="protein sequence ID" value="ABA05475"/>
        <property type="gene ID" value="Nwi_2221"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2221"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_22_5"/>
    <dbReference type="OMA" id="RLGDPFF"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="162"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="198"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="8">
        <location>
            <begin position="271"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="6">
        <location>
            <begin position="352"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="577"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="578"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="605" mass="65448" checksum="CDF169FD2AE08478" modified="2005-10-11" version="1">MSIIKDRLDALLHPSARYDALTRTRHRTFMAPRLLVSLAALAAFPIYLAIRGAPTALEVAAFAWLVVPILLSYFLSRTGRYETAHMLSSIALAGLVMMIAIPTGGIGSFATVWLVVIPLEAALSASRRVVAFASMLAAACLLALMAMSYLDVPPPDAGVAAHGGFMMFGVASALIYAAGVAFGSETLMRTSASMLNVEENRYRLLARHMCDVISRHRRNGAVQFISPAAESLLGVPVSELQGHGLFDRVHVADRPAYLTALAAAALGKGAQSVEFRLRRDAASDAEFEGHPSGNFIWVEMRCQPLEPSSPAEPEVVAVMRDVTDRKQQEHALDLARTAAERADAAKTRFLATMSHELRTPLNAIIGFSDMIVQEATLPLDATRRKEYAQLINDSGQHLLSVVNSILDMSKMESGSFEILPEPFAPREVLLSGSNLLALKARDNDIELVTRVPENLPQMVGDPRAFRQIVLNLVSNAIKFTERGGRVTISARVEGARMVLRVVDNGVGIAQEDLKRIGDPFFQAGKTYQRRHEGTGLGLSIVKSLVGLHGGEIDVQSKVEEGTTVTVMLPLTLMPAAEPPSNNIATLTPASRSGKQDQGYRVRKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQH1</accession>
    <name>Q3SQH1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2216</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315439.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05470">
        <property type="protein sequence ID" value="ABA05470"/>
        <property type="gene ID" value="Nwi_2216"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2216"/>
    <dbReference type="eggNOG" id="ENOG50314CR">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2524156_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229139" evidence="1">
        <location>
            <begin position="22"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="85" mass="8034" checksum="5B62F74239BE0D40" modified="2005-10-11" version="1">MKKILVLAAVVSAFMSAPAFANKGQGIGIGANVLTGRGGVVGLLNGRGALVVNTAVTTGKSGVLGAVLGRSLSSGGLLGGGCGCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQI0</accession>
    <name>Q3SQI0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">3-oxoadipate enol-lactonase</fullName>
            <ecNumber evidence="2">3.1.1.24</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2207</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.1.24" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315430.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2207"/>
    <dbReference type="ESTHER" id="nitwn-q3sqi0">
        <property type="family name" value="Carboxymethylbutenolide_lactonase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05461">
        <property type="protein sequence ID" value="ABA05461"/>
        <property type="gene ID" value="Nwi_2207"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2207"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_50_3_5"/>
    <dbReference type="OMA" id="MWRWQRE"/>
    <dbReference type="OrthoDB" id="1196738at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047570">
        <property type="term" value="F:3-oxoadipate enol-lactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042952">
        <property type="term" value="P:beta-ketoadipate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026968">
        <property type="entry name" value="PcaD/CatD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02427">
        <property type="entry name" value="protocat_pcaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="25"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="171"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="260" mass="28471" checksum="332DBF38BC7A139F" modified="2005-10-11" version="1">MPMIYADGCLLNVSVEGRDGGPTLMLSNALGCTLQMWEPQMAALSKLFRIIRYDRRGHGKSGVPPGPYSMERFGRDVLAILDDLNIARTHWCGLSMGGMVGQWLGAHAPERFDRIILANTNCYYPDPANWHARIKTVKEGGLAAIAETVISGWLTTGFREREPQITAKIKAMLSASPVEGYIACCEALSTLDQRELLPRIKSPTLVIAGRHDQSTPVAAGEYIRSRIPSASMTILDAAHISNVEQSHAFTEAVTGFLMQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQN1</accession>
    <name>Q3SQN1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">3-hydroxyisobutyrate dehydrogenase</fullName>
            <shortName evidence="5">HIBADH</shortName>
            <ecNumber evidence="5">1.1.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_2156</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>3-hydroxy-2-methylpropanoate + NAD(+) = 2-methyl-3-oxopropanoate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:17681"/>
            <dbReference type="ChEBI" id="CHEBI:11805"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57700"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid degradation; L-valine degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the HIBADH-related family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.31" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315379.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2156"/>
    <dbReference type="EnsemblBacteria" id="ABA05410">
        <property type="protein sequence ID" value="ABA05410"/>
        <property type="gene ID" value="Nwi_2156"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2156"/>
    <dbReference type="eggNOG" id="COG2084">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035117_6_0_5"/>
    <dbReference type="OMA" id="WSSEVNN"/>
    <dbReference type="OrthoDB" id="1245550at2"/>
    <dbReference type="UniPathway" id="UPA00362"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008442">
        <property type="term" value="F:3-hydroxyisobutyrate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006574">
        <property type="term" value="P:valine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002204">
        <property type="entry name" value="3-OH-isobutyrate_DH-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011548">
        <property type="entry name" value="HIBADH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015815">
        <property type="entry name" value="HIBADH-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029154">
        <property type="entry name" value="NADP-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14833">
        <property type="entry name" value="NAD_binding_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000103">
        <property type="entry name" value="HIBADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01692">
        <property type="entry name" value="HIBADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00895">
        <property type="entry name" value="3_HYDROXYISOBUT_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0101">Branched-chain amino acid catabolism</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="3 5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD_binding_2" evidence="6">
        <location>
            <begin position="4"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="NAD_binding_11" evidence="7">
        <location>
            <begin position="162"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000103-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU910714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA05410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="299" mass="30665" checksum="FCF98C3A56212BFC" modified="2005-10-11" version="1">MAAIAFIGLGNMGGPMAANLVKAGHSVVGFDLVPALCDAARTNGVTIAGSARAAVTNADVIITMLPAGRHVVSVWTDVMPAATRGALMIDCSTIDVESARRAHMLAARASLHSVDAPVSGGVAGARDATLTFMVGGEVESFEDAKPFLEAMGKKIVHCGRDGTGQAAKICNNMILGVSMIAVAEAFVLAEKLGLSHQALFDAASKASGQCWSLTSYCPVPGPVPTSPANNDYRPGFASALMLKDLTLARAAADSTGTVTPLGQHAQDIYKAFDAAGNGEVDFSGIIRHIRALAESSGDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQQ8</accession>
    <name>Q3SQQ8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Probably methylase/helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2128</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SBNO family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315354.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2128"/>
    <dbReference type="EnsemblBacteria" id="ABA05383">
        <property type="protein sequence ID" value="ABA05383"/>
        <property type="gene ID" value="Nwi_2128"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2128"/>
    <dbReference type="eggNOG" id="COG0553">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004986_1_0_5"/>
    <dbReference type="OMA" id="KVSPAWA"/>
    <dbReference type="OrthoDB" id="29618at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048583">
        <property type="term" value="P:regulation of response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026937">
        <property type="entry name" value="SBNO_Helicase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026741">
        <property type="entry name" value="SNO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039187">
        <property type="entry name" value="SNO_AAA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12706">
        <property type="entry name" value="PTHR12706"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13872">
        <property type="entry name" value="AAA_34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13871">
        <property type="entry name" value="Helicase_C_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AAA_34" evidence="3">
        <location>
            <begin position="412"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase_C_4" evidence="2">
        <location>
            <begin position="910"/>
            <end position="1167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1446" mass="156980" checksum="47FA3AEDE85FB9BC" modified="2005-10-11" version="1">MTPSTAFAAAASPLASSADIARCIDAAAHLLIPHLEQGRQVDAAILRAAMERGFGGSDASGAWDWKTAYDACEAATVLFLRKFGKAILRKAGSPALALSMLEKIVGLLPTHTRRSAESEALQQFSTPIPLGLAVATAAAVTPADRVLEPSAGTGLLAILPEIAGGSLVLNELAETRAGLLALLFPDIAVSRHDAAQIHDHLDPAIVPSVVIMNPPFSAMANVQGRMADAALRHIGSALARLAEGGRLVAITGANFAPDAPAWRDAYVRLQERGRVVFSAAIDGRVYARHGTTFPTRLIVIDKRPASDPTAFPDAPGVAPDVATLLAWIAKHVPARLPVDASVGLPAVTTPRTVRGYVARTAQAATRLSPAEPEGVELAYETIDWTPPQGARLSDSVYEDYALQSIRIPGAQDHPTQLVQSAAMASVAPPKPSYRPRLPANILSLLSGAQLETVIYAGEAHSGYLAGAWTVDQTFDVVTAAPEDAEGAVRFRRGFMIGDGTGVGKGREAAGIILDNWLRGRRKAVWISKSDKLIEDAQRDWSALGMERLLITPLSRFPQGKPITLREGILFLTYATLRSDDRGERLSRVKQIVEWLGSDFDGVIIFDEAHAMQNAVGARSERGDQEASQQGRAGLRLQHALPDARVVYVSATGATTVHNLSYAQRLGLWGSEDFPFSTRAEFVEAIEAGGVAAMEVLARDLRALGLYTSRSLSFDGVEYELVEHELTPEQTRIYDAYADAFAIIHNNLDAAMQAANITGGSEGRSGTLNRQAKAAARSAFESAKQRFFGHLLTSMKTPTLIRSIERDLEQGHAAVIQIVSTGEALMERRLAELPTEEWNDVRVDITPREYVLDYLAHSFPVQLYEPYTDSEGKLSSRPVYRDGQPVESREAVARRDALIAKLASLPPVPGALDQIVQHFGADMIAEVTGRSRRIVRKLGSAGRSDRLVVETRAASANLAEAQAFMDDHKRILVFSDAGGTGRSYHAELSARNTRLRVHYLLEPGWKADTAIQGLGRTHRTNQKQPPLFRPVATNVKAEKRFLSTIARRLDTLGAITRGQRATAGQGLFRPEDNLESHYARDALRQLYVLIARGRIAGCSLERFEAATGLTLTDANGLKDQLPGISTFLNRMLALTIDLQGVLFTAFEQLLNARIEGAIAAGTYDAGLETLRAESFVVTGRQTIHTHPGTGAETRLLTIMQRQRNRPLTLDEALSRLRERGARLLVNARSGHAAVQVHAPSVMLDDGEVEYRVRLIRPMEAPNVPVRTMAESHWKDADEASFAAAWTAELAEVPAFTDSTLHMVSGLLLPIWKRLPNESTRVYRLQTDDGERIIGRKVSPAWAATATATGLPSLTPDDAFAALIEGKTVIDLAEGLQLRCVRVMGGHRIELSGFTDTMRERLTAYGLFHEIISWKLRLFVPTDASGPAILARVMDRYPIERVAEREAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQS6</accession>
    <name>Q3SQS6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05365">
        <property type="protein sequence ID" value="ABA05365"/>
        <property type="gene ID" value="Nwi_2110"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2110"/>
    <dbReference type="HOGENOM" id="CLU_2168309_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="110" mass="12204" checksum="52522420E48E9264" modified="2005-10-11" version="1">MLADAQRASRAGLKVARLAVHARRADLICQTAAVSTGLIVTRRVTKLDRMRVRLHDGLRIQVVGTNASNQQQLIGENARDERDRSPMASVTQVPRRARIAPGCRQRSNPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQU7</accession>
    <name>Q3SQU7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2087</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2087"/>
    <dbReference type="EnsemblBacteria" id="ABA05344">
        <property type="protein sequence ID" value="ABA05344"/>
        <property type="gene ID" value="Nwi_2087"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2087"/>
    <dbReference type="eggNOG" id="ENOG5033X0K">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1433151_0_0_5"/>
    <dbReference type="OMA" id="IDNLAWI"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="189" mass="21505" checksum="3C68F30FB4356268" modified="2005-10-11" version="1">MTDATINIQSFIFNVFGAIDNLAWIWMAENGQKRADGTPIRDGYVGLGPDNTAVRGTLSQELQDYLKTLDDWFRYLAGLRHALAHRIPLYIPPYVIEAKDEAAYRDFEARMVEAGKKGDFTEYDRLSGEQLRLGRFRPWIQHSFQENAKPVVFHAQMLADFNTVDELARKMLGEYTSLADSGVAQVKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR09</accession>
    <name>Q3SR09_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cytochrome c domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2023</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315268.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2023"/>
    <dbReference type="EnsemblBacteria" id="ABA05282">
        <property type="protein sequence ID" value="ABA05282"/>
        <property type="gene ID" value="Nwi_2023"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2023"/>
    <dbReference type="eggNOG" id="COG1621">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014386_1_0_5"/>
    <dbReference type="OMA" id="RCGHEFG"/>
    <dbReference type="OrthoDB" id="52724at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229072" description="Cytochrome c domain-containing protein" evidence="5">
        <location>
            <begin position="22"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="6">
        <location>
            <begin position="326"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="602" mass="64530" checksum="17B76723B70358EC" modified="2005-10-11" version="1">MRTLILALALLNLPIASSSFADQSGPVFVDQGAAWDMSSRSDFYTRDQGSRLIPWAWLKALKQADGAPFLADGLTRYGYLPALDASTELPVGFYASGPTGAKVVGMTCSACHTRQIKVADRQYRIDGGPALVDFQAFLSDLDKAMARATSDDAAFKAFAASVLQTPKPDSDKVAALRSDVDAWYVRYHAIVDRSLPNPGWGLGRLDAVGMIFNRVTGLGLGPEPTFLIPENIHRADAPVRYPFLWNAPKQDKTQWPGFADNGSDILGLARNVGEVMGVFGNFQPTRDGLFIDFLNNNSVNFDGLQKLEDLAKKIGPPKWPWSVDAKLAAKGKTVFERPAADGGCQECHGQKAGKERFPFQKTWATPIQNVGTDTREYDILGWTAKSGVLKGAYIPFATKPLKETDLAFNILSTSVIGTIGEHVLSFSKGASPSALGASVQSEAAPGAPQPVDATQLPAVLQDLPGAFRFPEAAATPETRLRLDMGAGSSNTDAATPAPPKGAYESRVLYGIWAAAPYLHNGSVASLAELLKPASQRQQRFAIGSDYDVDNIGIAANQTQAGNVRETTGCDDLNSGNSRCGHEFGTSLPDADKKALLEYLKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR13</accession>
    <name>Q3SR13_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Short-chain dehydrogenase/reductase SDR</fullName>
            <ecNumber evidence="2">1.1.1.100</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2019</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.100" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315264.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2019"/>
    <dbReference type="EnsemblBacteria" id="ABA05278">
        <property type="protein sequence ID" value="ABA05278"/>
        <property type="gene ID" value="Nwi_2019"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2019"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_10_5"/>
    <dbReference type="OMA" id="SPIGHIE"/>
    <dbReference type="OrthoDB" id="1135906at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="250" mass="26628" checksum="EECC53DCA92C8237" modified="2005-10-11" version="1">MTKPLTSRVALVTGASRGIGYATARALAKAGAHVIAVARTQGGLEELDDDIRDGGGSATLVPLNLSDFDGIARLGAALNERHGKLDILVGNAGIAGPSSPLGHIEMKPWQDVMAINVTANFQLIRCMEPLLRQSDAGRAVFVTSGAAGKANAYRGPYAASKAALDTLVRCWASETATTGMRVNLFDPGPVRTRLRAIVFPGEDPMTLDTPEQIAELIIPMCLPSWSETGKLYDYKSRRLTDSRDRPVSNL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRD1</accession>
    <name>TIG_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Trigger factor</fullName>
            <shortName evidence="1">TF</shortName>
            <ecNumber evidence="1">5.2.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">PPIase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tig</name>
        <name type="ordered locus">Nwi_1900</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation. Functions as a peptidyl-prolyl cis-trans isomerase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">About half TF is bound to the ribosome near the polypeptide exit tunnel while the other half is free in the cytoplasm.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">Consists of 3 domains; the N-terminus binds the ribosome, the middle domain has PPIase activity, while the C-terminus has intrinsic chaperone activity on its own.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FKBP-type PPIase family. Tig subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315156.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRD1"/>
    <dbReference type="SMR" id="Q3SRD1"/>
    <dbReference type="STRING" id="323098.Nwi_1900"/>
    <dbReference type="PRIDE" id="Q3SRD1"/>
    <dbReference type="EnsemblBacteria" id="ABA05160">
        <property type="protein sequence ID" value="ABA05160"/>
        <property type="gene ID" value="Nwi_1900"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1900"/>
    <dbReference type="eggNOG" id="COG0544">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033058_2_2_5"/>
    <dbReference type="OMA" id="KGIKTQF"/>
    <dbReference type="OrthoDB" id="932993at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00303">
        <property type="entry name" value="Trigger_factor_Tig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005215">
        <property type="entry name" value="Trig_fac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008880">
        <property type="entry name" value="Trigger_fac_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037041">
        <property type="entry name" value="Trigger_fac_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008881">
        <property type="entry name" value="Trigger_fac_ribosome-bd_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036611">
        <property type="entry name" value="Trigger_fac_ribosome-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027304">
        <property type="entry name" value="Trigger_fact/SurA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30560">
        <property type="entry name" value="PTHR30560"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05698">
        <property type="entry name" value="Trigger_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05697">
        <property type="entry name" value="Trigger_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003095">
        <property type="entry name" value="Trigger_factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102735">
        <property type="entry name" value="SSF102735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109998">
        <property type="entry name" value="SSF109998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00115">
        <property type="entry name" value="tig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0697">Rotamase</keyword>
    <feature type="chain" id="PRO_0000256581" description="Trigger factor">
        <location>
            <begin position="1"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="1">
        <location>
            <begin position="171"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00303"/>
        </source>
    </evidence>
    <sequence length="453" mass="50852" checksum="377BBB1859A13A82" modified="2005-10-11" version="1">MQVNETLAEGLRHEFQVSIPAAEIAAKADERLDDLKDKVKINGFRPGKVPVSHLKKLYGRSVMVETIEDTIRDTNMQIVNDRGLKLAGNPKVTMPSEPKEIEDILAGNSDLSYSVAIEVVPPIELADFKSFTIEKPVVDVSDADVDEAVERIAEQNRTYTTRAEGAKAENGDRITISFKGTIDGELFEGGSSEDILVVLGSNALIPGFEEQLVGAGVGETRTVKASFPGNYADSELAGKDAEFETTVSLIEAPEELKIDDEFAKLLGLEELDQLRQVVREQLSAEFARAMRQHVKRALFDRLDETHKFDAPPTLVEEEFEQVWKTIMAEMEKEKKTFADENTTEEEARAEYRRIADRRVRLSLVLSEIGEKNGITVADDEINRALISRARQTPGREKEIWDYYQRNPQALAQIRAPLFENKVVDFILELATITEKSVTREDLFKEHEAHSAEM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRK4</accession>
    <name>ENO_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Enolase</fullName>
            <ecNumber evidence="1">4.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">2-phospho-D-glycerate hydro-lyase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">2-phosphoglycerate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">eno</name>
        <name type="ordered locus">Nwi_1827</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2-phosphoglycerate = H2O + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:10164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.2.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell surface</location>
        </subcellularLocation>
        <text evidence="1">Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the enolase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315083.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRK4"/>
    <dbReference type="SMR" id="Q3SRK4"/>
    <dbReference type="STRING" id="323098.Nwi_1827"/>
    <dbReference type="EnsemblBacteria" id="ABA05087">
        <property type="protein sequence ID" value="ABA05087"/>
        <property type="gene ID" value="Nwi_1827"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1827"/>
    <dbReference type="eggNOG" id="COG0148">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031223_2_1_5"/>
    <dbReference type="OMA" id="EFMIIPV"/>
    <dbReference type="OrthoDB" id="533698at2"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="PTHR11902"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001400">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00002">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="SSF51604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="SSF54826"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01060">
        <property type="entry name" value="eno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0964">Secreted</keyword>
    <feature type="chain" id="PRO_0000267064" description="Enolase">
        <location>
            <begin position="1"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="337"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent; in inhibited form" evidence="1">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="364"/>
            <end position="367"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="388"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00318"/>
        </source>
    </evidence>
    <sequence length="426" mass="45104" checksum="4FAD4C1B9316CAC4" modified="2005-10-11" version="1">MTAIVDIIGREILDSRGNPTVEVDVVLEDGAMGRAAVPSGASTGAYEAVELRDGDNSRYHGKGVRKAVDAVNGEIFDAIGGMDAEQQAQIDETLIELDGTANKGRLGANAILGVSLALARAAADSLDIPLYRYVGGVTARTLPVPMMNIVNGGVHADNPIDFQEFMIMPVGVQTFSDALRCGSEIFHTLRSELKKAGHNTNVGDEGGFAPDLPSAGAALDFIMGAIVKAGYRPGDDVALALDPASTEFFKDGKYVYGGEGKTRSIEEQAQYLAKLASDYPIVSIEDGMAEDDFEGWKLVTDMIGKTCQLVGDDLFVTNVTRLADGIRNGRANSILIKVNQIGTLTETLAAVEMAYKAGYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSLSRSDRAAKYNQLLRIEQQLGAQAKYAGRAALKAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRL6</accession>
    <name>Q3SRL6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">OmpA/MotB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_1814</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315071.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1814"/>
    <dbReference type="EnsemblBacteria" id="ABA05075">
        <property type="protein sequence ID" value="ABA05075"/>
        <property type="gene ID" value="Nwi_1814"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1814"/>
    <dbReference type="eggNOG" id="COG2885">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055761_0_0_5"/>
    <dbReference type="OMA" id="PEMHVDL"/>
    <dbReference type="OrthoDB" id="583204at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07185">
        <property type="entry name" value="OmpA_C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032608">
        <property type="entry name" value="DUF4892"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006664">
        <property type="entry name" value="OMP_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006665">
        <property type="entry name" value="OmpA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036737">
        <property type="entry name" value="OmpA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16234">
        <property type="entry name" value="DUF4892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00691">
        <property type="entry name" value="OmpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01021">
        <property type="entry name" value="OMPADOMAIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103088">
        <property type="entry name" value="SSF103088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51123">
        <property type="entry name" value="OMPA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229171" evidence="3">
        <location>
            <begin position="20"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="OmpA-like" evidence="4">
        <location>
            <begin position="204"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="321" mass="35546" checksum="B77E8D29BFBF5ACC" modified="2005-10-11" version="1">MRTFLMSLGVVLVAASVQAADIKGSKDHPLLPRYDGSEILRYKQEAFTDYKLATALIKNYGGKDKNANSVTPLEGKFTQIAYKMPEGRSSLEVFRNYEQALKKAGFETMFSCERETCGGRNFSLAIAGDPLYMLFGDQQADQRYVAARLKRAEGDVYASLYVIMHRSGGGPLKDRALALLEITEAKPMEERMVVVESSAMQRDIASQGRVAIHGILFDFDKDTMRSDSKPQLDEIAKLLKAQPELKVLIVGHTDSKGALDYNRDLSYRRARSIVESLSREHGVAAARLTPLGVGMAAPMATNRTEEGRALNRRVELVDATN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRR3</accession>
    <name>Q3SRR3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1767</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315024.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1767"/>
    <dbReference type="EnsemblBacteria" id="ABA05028">
        <property type="protein sequence ID" value="ABA05028"/>
        <property type="gene ID" value="Nwi_1767"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1767"/>
    <dbReference type="eggNOG" id="COG3737">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074390_2_1_5"/>
    <dbReference type="OMA" id="QIREAHF"/>
    <dbReference type="OrthoDB" id="1948784at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036748">
        <property type="entry name" value="MTH938-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007523">
        <property type="entry name" value="NDUFAF3/AAMDC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21192">
        <property type="entry name" value="PTHR21192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04430">
        <property type="entry name" value="DUF498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64076">
        <property type="entry name" value="SSF64076"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="128" mass="13777" checksum="BD5605E618F29614" modified="2005-10-11" version="1">MSAAPPEVPHLPRPAAIEAYGNGGFRFDTMSHRGSLLCLPDGVWAWPVARADDIDRHALARVFEGSAAIDTLLIGTGINVWLVPPELRAALRRVSIGVDTMLTGPAIRTYNIMLGERRRVAAALIAVP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRV4</accession>
    <name>NRDR_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcriptional repressor NrdR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nrdR</name>
        <name type="ordered locus">Nwi_1726</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Negatively regulates transcription of bacterial ribonucleotide reductase nrd genes and operons by binding to NrdR-boxes.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NrdR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314983.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRV4"/>
    <dbReference type="SMR" id="Q3SRV4"/>
    <dbReference type="STRING" id="323098.Nwi_1726"/>
    <dbReference type="EnsemblBacteria" id="ABA04987">
        <property type="protein sequence ID" value="ABA04987"/>
        <property type="gene ID" value="Nwi_1726"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1726"/>
    <dbReference type="eggNOG" id="COG1327">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108412_0_1_5"/>
    <dbReference type="OMA" id="YRFTTYE"/>
    <dbReference type="OrthoDB" id="1551627at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00440">
        <property type="entry name" value="NrdR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003796">
        <property type="entry name" value="RNR_NrdR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30455">
        <property type="entry name" value="PTHR30455"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00244">
        <property type="entry name" value="TIGR00244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0678">Repressor</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000230875" description="Transcriptional repressor NrdR">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-cone" evidence="1">
        <location>
            <begin position="49"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="zinc finger region" evidence="1">
        <location>
            <begin position="3"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="160" mass="18619" checksum="2A52378C6BC96661" modified="2005-10-11" version="1">MRCPSCNSLDTQVKDSRPTEDSAVIRRRRVCMACNFRFTTFERVQLRELTVIKRNGRRVPFDRDKLVRSLQISLRKRPVEPERIETMVSAIVRELESGGEAEISSETIGEIVMEHLRSLDDVAYVRFASVYRNFREAKDFEAVLGELSSEDDPRRVPLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRW6</accession>
    <name>Q3SRW6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1714</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314973.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1714"/>
    <dbReference type="EnsemblBacteria" id="ABA04975">
        <property type="protein sequence ID" value="ABA04975"/>
        <property type="gene ID" value="Nwi_1714"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1714"/>
    <dbReference type="eggNOG" id="COG5349">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133751_1_0_5"/>
    <dbReference type="OMA" id="QWAFRMH"/>
    <dbReference type="OrthoDB" id="2075088at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009325">
        <property type="entry name" value="DUF983"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06170">
        <property type="entry name" value="DUF983"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="146" mass="16263" checksum="70C89019B5859880" modified="2005-10-11" version="1">MTALPPDTASTAGKTWTPEGEKRDTWAAMRRGLFCRCPRCGQGKLFRTFLKTADNCSECGLDFTPHRADDLPAYLVIVVVGHIVVPLALLIEKAFAPPLILQWAIYLPLTLIMALWLIQPIKGAVVGLQWALRMHGFDENFVDDQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRZ2</accession>
    <name>Q3SRZ2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3-oxoacyl-[acyl-carrier-protein] reductase</fullName>
            <ecNumber evidence="3">1.1.1.100</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1688</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a (3R)-hydroxyacyl-[ACP] + NADP(+) = a 3-oxoacyl-[ACP] + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:17397"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="1.1.1.100"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.100" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314947.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1688"/>
    <dbReference type="EnsemblBacteria" id="ABA04949">
        <property type="protein sequence ID" value="ABA04949"/>
        <property type="gene ID" value="Nwi_1688"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1688"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_5"/>
    <dbReference type="OMA" id="KMPERDY"/>
    <dbReference type="OrthoDB" id="1601931at2"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011284">
        <property type="entry name" value="3oxo_ACP_reduc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01830">
        <property type="entry name" value="3oxo_ACP_reduc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="2 3" id="KW-0521">NADP</keyword>
    <keyword evidence="3 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="152"/>
            <end position="156"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR611284-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR611284-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU366074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="245" mass="25640" checksum="E9FE93684D12DDDA" modified="2005-10-11" version="1">MFDLTDRTALVTGATGGIGGAIAKALHAQGATVAISGTRREVLETLADTLGERVHVLPCNLSDTAAVEALVPDAEKAMGKVDILVANAGVTRDNLFVQLRDEDWDDVIRINLTATFRLARAATKLMMRKRFGRIIAITSVVGVTGNPGQGNYTASKAGLIGMIKTLGAEYAKRGVTANCIAPGFIATAMTDALNEKQRETILTKVPAARLGTPEDIAAAAVYLSSNEAAYVTGQTIHVNGGMAMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS09</accession>
    <name>Q3SS09_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Peptidase M23B</fullName>
            <ecNumber evidence="4">3.4.24.75</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1671</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.4.24.75" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314931.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1671"/>
    <dbReference type="EnsemblBacteria" id="ABA04932">
        <property type="protein sequence ID" value="ABA04932"/>
        <property type="gene ID" value="Nwi_1671"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1671"/>
    <dbReference type="eggNOG" id="COG0739">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029425_2_1_5"/>
    <dbReference type="OMA" id="HYETRID"/>
    <dbReference type="OrthoDB" id="1891666at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016047">
        <property type="entry name" value="Peptidase_M23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01551">
        <property type="entry name" value="Peptidase_M23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51261">
        <property type="entry name" value="SSF51261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M23" evidence="3">
        <location>
            <begin position="347"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="454" mass="49119" checksum="43D8C51CC2AC8A98" modified="2005-10-11" version="1">MSYRSSQCVEYPPQRAHAHKHDRSPSRRAAAGLSSGAPEKRSGYTFAHGGRQVRFGPVVFWIAVGTVTLLGVWSAATATYFAFRDDVLTRLLARQAEMQYAYEDRIAELRSRVDRTTSRQLLDQEQFERKLDGIMRRQALLEARAGALNAISDVSVTGSIKGAAQKTGNDTDAGPSRADKPSPINDADGFEMPDRGAALDSRDPANARPKPVPLMNLTGEANRLTRLESSLDRVESRQVAELNAIADGMDSRLRRMRGVFSDLGLSVAKMAAVSNSGTGGPYVPVKLRSDVDPFERQLYRVSLTRAQIDQLDRALALVPYRKPVTGELEFTSGFGVRVDPFVGRPAMHAGLDFRAATGAPARATANGKVTTANWTGGYGRMVEVDHGNGLSTRYGHLSAINVKVGQSVKAGQAVGEVGSTGRSTGPHLHYETRIDGEAVDPQKFLRAGARLNNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS19</accession>
    <name>Q3SS19_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 9">Cysteine desulfurase</fullName>
            <ecNumber evidence="5 9">2.8.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_1661</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, L-selenocysteine, and L-selenocystine to produce L-alanine.</text>
    </comment>
    <comment type="function">
        <text evidence="3">Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>[sulfur carrier]-H + L-cysteine = [sulfur carrier]-SH + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:43892"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="EC" id="2.8.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.7" evidence="5 9"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314921.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1661"/>
    <dbReference type="EnsemblBacteria" id="ABA04922">
        <property type="protein sequence ID" value="ABA04922"/>
        <property type="gene ID" value="Nwi_1661"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1661"/>
    <dbReference type="eggNOG" id="COG0520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003433_2_5_5"/>
    <dbReference type="OMA" id="HKLCGPT"/>
    <dbReference type="OrthoDB" id="446447at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031071">
        <property type="term" value="F:cysteine desulfurase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006534">
        <property type="term" value="P:cysteine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06453">
        <property type="entry name" value="SufS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010970">
        <property type="entry name" value="Cys_dSase_SufS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016454">
        <property type="entry name" value="Cysteine_dSase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005572">
        <property type="entry name" value="NifS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01979">
        <property type="entry name" value="sufS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_5" evidence="10">
        <location>
            <begin position="33"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA04922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="414" mass="45609" checksum="CEA9885EB9977BD8" modified="2005-10-11" version="1">MTHPAVANGSYDVMRVREDFPALAMKVYGKPLVYLDNAASAQKPNAVLDRMAEAYKTEYANVHRGLHYLANAATEAYEGGRARVARFLNAGRTEEIIFTRNASEAINLVASSWGEPNIKAGDEIVLSIMEHHSNIVPWHFLRERHGAVIKWAPVDDDGNFLIEEFEKLLTPKTKLVAITQMSNALGTLVPVKDVVKLAHARDIPVLVDGSQAAVHLAIDVQDIDCDFYVFTGHKLYGPTGIGALYAKHDHLVSMRPFNGGGEMIREVAQDWVTYGDPPHKFEAGTPAIVESIGLGAAIDYVNSIGKERIAAHEHDLLTYAQERLREINSLRIIGTARDKGPVISFEMKGAHPHDVATVIDRAGIAVRAGTHCVMPLLERFNVSATCRASFGMYNTREEIDHLAQALIKARELFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS31</accession>
    <name>Q3SS31_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1649</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1649"/>
    <dbReference type="EnsemblBacteria" id="ABA04910">
        <property type="protein sequence ID" value="ABA04910"/>
        <property type="gene ID" value="Nwi_1649"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1649"/>
    <dbReference type="eggNOG" id="ENOG50313JS">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_169681_1_0_5"/>
    <dbReference type="OMA" id="AMDPAEQ"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="101" mass="10757" checksum="A4575A813825CF09" modified="2005-10-11" version="1">MTTESQGQTSRRAAPVAVRIAALAVLMSLALAGCSTSIADFETSSDAPERPRDVSSYPAVHDLPAPRDQAAMDPAEQAKVEKELIDARDRQAHATQNAEPQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS37</accession>
    <name>EFP_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Elongation factor P</fullName>
            <shortName evidence="1">EF-P</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">efp</name>
        <name type="ordered locus">Nwi_1643</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein biosynthesis; polypeptide chain elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the elongation factor P family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314903.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SS37"/>
    <dbReference type="SMR" id="Q3SS37"/>
    <dbReference type="STRING" id="323098.Nwi_1643"/>
    <dbReference type="EnsemblBacteria" id="ABA04904">
        <property type="protein sequence ID" value="ABA04904"/>
        <property type="gene ID" value="Nwi_1643"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1643"/>
    <dbReference type="eggNOG" id="COG0231">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074944_1_1_5"/>
    <dbReference type="OMA" id="RWRTTEQ"/>
    <dbReference type="OrthoDB" id="1260763at2"/>
    <dbReference type="UniPathway" id="UPA00345"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04470">
        <property type="entry name" value="S1_EF-P_repeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05794">
        <property type="entry name" value="S1_EF-P_repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00141">
        <property type="entry name" value="EF_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015365">
        <property type="entry name" value="Elong-fact-P_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020599">
        <property type="entry name" value="Transl_elong_fac_P/YeiP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013185">
        <property type="entry name" value="Transl_elong_KOW-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001059">
        <property type="entry name" value="Transl_elong_P/YeiP_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013852">
        <property type="entry name" value="Transl_elong_P/YeiP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011768">
        <property type="entry name" value="Transl_elongation_fac_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30053">
        <property type="entry name" value="PTHR30053"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01132">
        <property type="entry name" value="EFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08207">
        <property type="entry name" value="EFP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09285">
        <property type="entry name" value="Elong-fact-P_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005901">
        <property type="entry name" value="EF-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01185">
        <property type="entry name" value="EFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00841">
        <property type="entry name" value="Elong-fact-P_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00038">
        <property type="entry name" value="efp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01275">
        <property type="entry name" value="EFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0251">Elongation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000010795" description="Elongation factor P">
        <location>
            <begin position="1"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00141"/>
        </source>
    </evidence>
    <sequence length="188" mass="20732" checksum="F886F5B5C8C7EF77" modified="2005-10-11" version="1">MRVIASSIRKGNVIEQDGKLYVVLTAENIHPGKGTPVSQIEMRRISDGVKISERYKTTDQVERATIEDHNFTFLYEDGDGFHFMNAETYDQVQVPKDIVGSAAPYLQENMVVKLSLHDMVPVAITLPQRVTLEVVETEPVTKGQTASSSYKPAVLSNGVRTGVPPHVAVGTRVVVMTEDGSYVERAKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS85</accession>
    <name>Q3SS85_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulatory inactivation of DnaA Hda protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1595</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314860.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1595"/>
    <dbReference type="EnsemblBacteria" id="ABA04856">
        <property type="protein sequence ID" value="ABA04856"/>
        <property type="gene ID" value="Nwi_1595"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1595"/>
    <dbReference type="eggNOG" id="COG0593">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072265_0_0_5"/>
    <dbReference type="OMA" id="DRWPDWP"/>
    <dbReference type="OrthoDB" id="1110566at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013317">
        <property type="entry name" value="DnaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00308">
        <property type="entry name" value="Bac_DnaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_DnaA" evidence="1">
        <location>
            <begin position="97"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="226" mass="24559" checksum="0CCDFA3326186323" modified="2005-10-11" version="1">MAGRPPSRQLAFTLPHAESFSRDDFLEGPANAAALSLIESWPEWPNRIMLLAGPEGCGKSHLAAIWAERAGARSISAQGLTAATVPMALTTGALVVEDLNPKTFDELALFHLMNLAREEAAFVLMTARVTPAAIEIGLRDLRSRLRAVPVVTLMPPDDHLLRALIVKFSADRQMNIDEAIVSFIATRTERSVAAARLAVEQLDAESLRLGRPVTRALAAELLRNHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSB7</accession>
    <name>Q3SSB7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1563</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314830.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1563"/>
    <dbReference type="EnsemblBacteria" id="ABA04824">
        <property type="protein sequence ID" value="ABA04824"/>
        <property type="gene ID" value="Nwi_1563"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1563"/>
    <dbReference type="eggNOG" id="ENOG50348EK">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2481292_0_0_5"/>
    <dbReference type="OMA" id="NAYFALP"/>
    <dbReference type="OrthoDB" id="2073139at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="89" mass="10108" checksum="E8CEA845EDC87DB3" modified="2005-10-11" version="1">MKRRKTGRLAMRCEGKFWNAYYALPDTMEDAILLGSIHIRLVADVTRKNLFMALMQEAVSDMLTDITGTRPTWPDEPHAAPPHERAGHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSI5</accession>
    <name>Q3SSI5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1495</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04756">
        <property type="protein sequence ID" value="ABA04756"/>
        <property type="gene ID" value="Nwi_1495"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1495"/>
    <dbReference type="eggNOG" id="ENOG50322UX">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1376900_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="198" mass="22156" checksum="56C5FFF49DB8281D" modified="2005-10-11" version="1">MTADVRLMSLLGALRNFNDYITASGRLSAESLANQIRIERILADKVMFNMGWYAAREIAGDLKGAAATEAVLPLLFKAAELANSLADATERCEALDAKLAELNRPGRKKGSYEKWHFDPGYSVVVAMIETRPEKRQSDHVRWAVKNGWLDKSIPDKTHIRRIGLIQRRQEAERAAQLASLGANVIPFKSNRNPQKRAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSK5</accession>
    <name>Q3SSK5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phage head-tail adaptor, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1475</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1475"/>
    <dbReference type="EnsemblBacteria" id="ABA04736">
        <property type="protein sequence ID" value="ABA04736"/>
        <property type="gene ID" value="Nwi_1475"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1475"/>
    <dbReference type="eggNOG" id="COG5614">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147810_6_1_5"/>
    <dbReference type="OMA" id="WIDEITT"/>
    <dbReference type="OrthoDB" id="2081290at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008767">
        <property type="entry name" value="Phage_SPP1_head-tail_adaptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038666">
        <property type="entry name" value="SSP1_head-tail_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05521">
        <property type="entry name" value="Phage_H_T_join"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01563">
        <property type="entry name" value="gp16_SPP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="104" mass="11901" checksum="FFEEE65D5B8C24F2" modified="2005-10-11" version="1">MRAGKLDQQIAIQRFTNTVDDYGTPAETWTTLATVRAQIIQQSTEEFIRNFGASDETVTVFRVRWIDEITTADRVLFDGDNHNIKETKQIGRRAGLELRCQKIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="134" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSL8</accession>
    <name>Q3SSL8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Methionine--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Methionyl-tRNA synthetase</fullName>
            <shortName evidence="8">MetRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">metG</name>
        <name evidence="13" type="ordered locus">Nwi_1462</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)</text>
            <dbReference type="Rhea" id="RHEA:13481"/>
            <dbReference type="Rhea" id="RHEA-COMP:9667"/>
            <dbReference type="Rhea" id="RHEA-COMP:9698"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.10"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.10" evidence="8"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1462"/>
    <dbReference type="EnsemblBacteria" id="ABA04723">
        <property type="protein sequence ID" value="ABA04723"/>
        <property type="gene ID" value="Nwi_1462"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1462"/>
    <dbReference type="eggNOG" id="COG0143">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009710_9_3_5"/>
    <dbReference type="OMA" id="SDMHGTP"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07957">
        <property type="entry name" value="Anticodon_Ia_Met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00814">
        <property type="entry name" value="MetRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01228">
        <property type="entry name" value="Met_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041872">
        <property type="entry name" value="Anticodon_Met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014758">
        <property type="entry name" value="Met-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023457">
        <property type="entry name" value="Met-tRNA_synth_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033911">
        <property type="entry name" value="MetRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43326">
        <property type="entry name" value="PTHR43326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19303">
        <property type="entry name" value="Anticodon_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01041">
        <property type="entry name" value="TRNASYNTHMET"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00398">
        <property type="entry name" value="metG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA-synt_1g" evidence="11">
        <location>
            <begin position="184"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA-synt_1g" evidence="11">
        <location>
            <begin position="334"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="Anticodon_3" evidence="12">
        <location>
            <begin position="552"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="8">
        <location>
            <begin position="477"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="61"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF09334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF19303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA04723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="692" mass="76373" checksum="B38F81A109CCEE46" modified="2005-10-11" version="1">MVAPAKKKTAKKPRVKSRKPRSAATGRNPKAKTRGKTAVKKTTKRKKTPAKGTTTARGPKPARKAAKKKTLKKAARKTTKRTVRKAARKPAKKQPATRTPKKVASKKTASKKVAPKKPTAKKVTPRKTAPRKAAPEKAARKRSVTTPPVKKTKPRAPRKVAAKVDTAAASLPSSPASSRADTFYITTAIVYPNGAPHIGHAYEAIATDALARFQRLDGKDVFFLTGTDEHGQKMVQTAQRENISALELATRNAELLRKLDEALDISFDRFIRTSESAHHRSVQDIWKRMAGNGDIYLSNYSGWYSVRDEAYYAEDETVVGEDNVRRGPQGTPVEWVEEKSYFFKLSAYEDRLLALYESEPDFIGPDSRRNEVVSFVKGGLKDLSISRTTFDWGVKVPGDPEHVMYVWVDALTNYITGVGFPDEDESNWRFWPADIHIIGKDIIRFHAVYWPAFLMSAGIALPKRVYAHGFLFNRGEKMSKSVGNVVDPFSLAAQYGVDQMRYFFLREVPFGQDGSYNHEAIVARINADLANDFGNLAQRSLSMIAKQHGGVLPEPGDFSDADKAILAQADGMIAIAREAMNTQQIHQALNAIWSVVAEANRYFAGEAPWALAKTDPARQKTVLYVTAEVVRQIAILAQPAMPAACAKLLDILGIPADERNFAMLGGDIRIRPGSALPAPVPVFPRYVEPPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSN2</accession>
    <name>Q3SSN2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Probable branched-chain-amino-acid aminotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1448</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acts on leucine, isoleucine and valine.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314717.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1448"/>
    <dbReference type="EnsemblBacteria" id="ABA04709">
        <property type="protein sequence ID" value="ABA04709"/>
        <property type="gene ID" value="Nwi_1448"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1448"/>
    <dbReference type="eggNOG" id="COG0115">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020844_4_1_5"/>
    <dbReference type="OMA" id="RGFQFAD"/>
    <dbReference type="OrthoDB" id="885013at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="SSF56752"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="285" mass="31761" checksum="5B6F0DB84D6165F4" modified="2005-10-11" version="1">MSRIAYVNGRYRDLRDACVNVEDRGYQFADGVYEVCEVRGARLVDFPRHMARLQRSMRELRIAEPMPITALGIIMREVVRRNRVTYGIVYLQVTRGVARRDHAFPPNPVKPGVVVTARALDDEKKQQAAAAGISVITLPENRWPRVDIKSVALLPNVLAKQHAREKGAHEAWYVDSDGFVTEGASSNAWIVTKDGRAVTRSVDQGILAGITRAVLMEVLRAFQIRLEERPFTPAEAREAAEAFVTSASQIVMPVVAIDGQVVGDGRPGGIARRLREEFHRFSAFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="126" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSN6</accession>
    <name>Q3SSN6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_1444</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314713.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1444"/>
    <dbReference type="EnsemblBacteria" id="ABA04705">
        <property type="protein sequence ID" value="ABA04705"/>
        <property type="gene ID" value="Nwi_1444"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1444"/>
    <dbReference type="eggNOG" id="COG3852">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_39_5"/>
    <dbReference type="OMA" id="EIMLTTA"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histidine kinase" evidence="5">
        <location>
            <begin position="146"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="372"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA04705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="391" mass="42747" checksum="095827058F76639C" modified="2005-10-11" version="1">MTSIAQRPRVASSDGEAILNALPNPVLLVAPDGRIVDANMAAESFFEMSKQFLQREPLKQLIPFGSPLLALIDQVRDSGAPVNEFRVDLGTPRIGRDRQVDLHVAPLTERPAHVVVMIQERTIADKMDRQLTHRSAARSVIALAAMLAHEIKNPLSGIRGAAQLLEQGATPDDRMLTRLICDEADRIVTLVDRMEVFGDERPIRREPVNIHAVLDHVKRLAQSGFARNVRFTVDYDPSLPPVLGDRDQLVQIFLNLVKNAAEAVSDLGEEGEIQFTTAFRPGLRLSLPGKKTRVSLPLEFCVKDNGPGVAEDLLPNLFDPFVTTKQTGSGLGLALVAKIVGDHGGIVECESQPRKTNFRILLPMFDSGPVHRQGDDAAASGIPPRTARHPA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSP4</accession>
    <name>SYV_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Valine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Valyl-tRNA synthetase</fullName>
            <shortName evidence="1">ValRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">valS</name>
        <name type="ordered locus">Nwi_1436</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val)</text>
            <dbReference type="Rhea" id="RHEA:10704"/>
            <dbReference type="Rhea" id="RHEA-COMP:9672"/>
            <dbReference type="Rhea" id="RHEA-COMP:9708"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78537"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.9"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">The C-terminal coiled-coil domain is crucial for aminoacylation activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.9" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314705.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSP4"/>
    <dbReference type="SMR" id="Q3SSP4"/>
    <dbReference type="STRING" id="323098.Nwi_1436"/>
    <dbReference type="EnsemblBacteria" id="ABA04697">
        <property type="protein sequence ID" value="ABA04697"/>
        <property type="gene ID" value="Nwi_1436"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1436"/>
    <dbReference type="eggNOG" id="COG0525">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001493_0_2_5"/>
    <dbReference type="OMA" id="FATKLWN"/>
    <dbReference type="OrthoDB" id="32262at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004832">
        <property type="term" value="F:valine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006438">
        <property type="term" value="P:valyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07962">
        <property type="entry name" value="Anticodon_Ia_Val"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02004">
        <property type="entry name" value="Val_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033705">
        <property type="entry name" value="Anticodon_Ia_Val"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037118">
        <property type="entry name" value="Val-tRNA_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019499">
        <property type="entry name" value="Val-tRNA_synth_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002303">
        <property type="entry name" value="Valyl-tRNA_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946">
        <property type="entry name" value="PTHR11946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10458">
        <property type="entry name" value="Val_tRNA-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00986">
        <property type="entry name" value="TRNASYNTHVAL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="SSF46589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="SSF50677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00422">
        <property type="entry name" value="valS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000224517" description="Valine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="894"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="571"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="574"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02004"/>
        </source>
    </evidence>
    <sequence length="952" mass="107385" checksum="94413800F10B093E" modified="2005-10-11" version="1">MIEKTYQPSDIESRMSRVWEEAGAFKAGRPERREAEPFTIVIPPPNVTGSLHMGHALNNTLQDVLCRFERMRGRDVLWQPGTDHAGIATQMVVERQLMERKEPGRREMGRARFLERVWQWKAESGGVIVNQLKRLGASCDWSRERFTMDEGLSRAVAKVFVELHRAGLIYKDKRLVNWDPKLLTAISDLEVKQVEVKGSLWHLRYPIEGKAFDPSDPSTYIVVATTRPETMLGDTAVAVHPENAKLEYLIGSNVVLPLAGRIIPIVGDDYADPEKGTGAVKITPAHDFNDFEVGRRHHLPQISVLDREGRLTLSENEDFLRGLPSGALMLAEEFDGMDRFAARKAIVARLEEFGFLDKIEPHTHMVPHGDRSNAVVEPYLTDQWYVDAKELARPAMAAVRSGETAFVPKNWEKTYFEWMENIQPWCISRQLWWGHQIPAWYGPDGKVFVAETEDEAIGHALGYYVEQGVITAEQGAGMARDPAKRDGFITRDEDVLDTWFSSALWPFSTLGWPDETPEVRRYYPTNVLVTGFDIIFFWVARMMMMGIHFMKEAPFSTVYIHALVRDEKGAKMSKSKGNVIDPLNLVDKYGADALRFTLAAMAVQGRDIKLSPQRVEGYRNFATKFWNACRFAEMNDCVVPARFDPTAATETLNRWIVHETARTACEVTEAIESSRFNDAASAIYRFVWNVYCDWYLELAKPVILGEDSPAKSETRAMVAWARDEILKLLHPFMPFITEELWAVTAERTRLLTLTEWPNKADQTRKRRTLIAAADPFIGSEPITDLLEPYFRDDAAEAEIGWVVDLVTAIRSVRAEMNIPPATLAPLVLAGASDESRARAQRWSDVIKRMSRLADISFADQAPAGAVQLLIRGEVAALPLKGIVDVAAQRTRLGKEIAKADADIARVDLKLADQNFIANAPGEIVEDEKEKREAAAARKAKFVEALERLKAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSS7</accession>
    <name>Q3SSS7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">SmpA/OmlA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_1403</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314673.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1403"/>
    <dbReference type="EnsemblBacteria" id="ABA04664">
        <property type="protein sequence ID" value="ABA04664"/>
        <property type="gene ID" value="Nwi_1403"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1403"/>
    <dbReference type="eggNOG" id="COG2913">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104933_1_0_5"/>
    <dbReference type="OMA" id="VIAIYFD"/>
    <dbReference type="OrthoDB" id="1813418at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026592">
        <property type="entry name" value="BamE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037873">
        <property type="entry name" value="BamE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007450">
        <property type="entry name" value="Lipoprotein_SmpA/OmlA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37482">
        <property type="entry name" value="PTHR37482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04355">
        <property type="entry name" value="SmpA_OmlA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="SmpA_OmlA" evidence="5">
        <location>
            <begin position="53"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA04664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="166" mass="18268" checksum="C6171E380735DE23" modified="2005-10-11" version="1">MQEDEGGPRMTVSVLNKAPGNPATRRHRLRAALAATLVCAVMAGCTGETFQKGYILPPGALEQIQIGASQDQVLIVMGTPSTVATLNGEVFYYISQRAERKVAFMPQKVIDQRVIAIYFDNNRQVTRLANYGLKDGKIFDFISRTTPTSGQEISYLQPLFKLLSAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SST3</accession>
    <name>Q3SST3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ISBm1, transposase orfA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1397</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1397"/>
    <dbReference type="EnsemblBacteria" id="ABA04658">
        <property type="protein sequence ID" value="ABA04658"/>
        <property type="gene ID" value="Nwi_1397"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1397"/>
    <dbReference type="eggNOG" id="COG3293">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055261_2_2_5"/>
    <dbReference type="OMA" id="PPSQTCY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025161">
        <property type="entry name" value="Put_IS4/IS5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13340">
        <property type="entry name" value="DUF4096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="121" mass="13783" checksum="B3D03E14634C6430" modified="2005-10-11" version="1">MARFDLTDFEWSVIQPLLPKKSRGVARVDDRRVLNGIFWRLRTGAPWADIPARYGPHTTCVNRFNRWRKAGVWDRILSAVSKAYDGGIQMIDSSSIRVHQHAANGQKNRRDPVAWVARAAG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-01-23" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSU8</accession>
    <name>RL30_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L30</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpmD</name>
        <name type="ordered locus">Nwi_1382</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314656.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSU8"/>
    <dbReference type="SMR" id="Q3SSU8"/>
    <dbReference type="STRING" id="323098.Nwi_1382"/>
    <dbReference type="EnsemblBacteria" id="ABA04643">
        <property type="protein sequence ID" value="ABA04643"/>
        <property type="gene ID" value="Nwi_1382"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1382"/>
    <dbReference type="eggNOG" id="COG1841">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131047_1_2_5"/>
    <dbReference type="OMA" id="GKQNQRD"/>
    <dbReference type="OrthoDB" id="2084745at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01658">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01371_B">
        <property type="entry name" value="Ribosomal_L30_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="L30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005996">
        <property type="entry name" value="Ribosomal_L30_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_L30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002211">
        <property type="entry name" value="Ribosomal_L30_bac-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="SSF55129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01308">
        <property type="entry name" value="rpmD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000273814" description="50S ribosomal protein L30">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="64" mass="7175" checksum="FDD0D71D0FDE3474" modified="2005-10-11" version="1">MAKAAKTIKVEQTGSAIRRHHSQRSTLVGLKLNKIGRTSELQDTPEIRGMIAKVRHIVRVIDET</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSV3</accession>
    <name>RS14_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsN</name>
        <name type="ordered locus">Nwi_1377</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds 16S rRNA, required for the assembly of 30S particles and may also be responsible for determining the conformation of the 16S rRNA at the A site.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Contacts proteins S3 and S10.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314651.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSV3"/>
    <dbReference type="SMR" id="Q3SSV3"/>
    <dbReference type="STRING" id="323098.Nwi_1377"/>
    <dbReference type="EnsemblBacteria" id="ABA04638">
        <property type="protein sequence ID" value="ABA04638"/>
        <property type="gene ID" value="Nwi_1377"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1377"/>
    <dbReference type="eggNOG" id="COG0199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139869_0_1_5"/>
    <dbReference type="OMA" id="FGLCRNQ"/>
    <dbReference type="OrthoDB" id="2032609at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00537">
        <property type="entry name" value="Ribosomal_S14_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001209">
        <property type="entry name" value="Ribosomal_S14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023036">
        <property type="entry name" value="Ribosomal_S14_bac/plastid"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19836">
        <property type="entry name" value="PTHR19836"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00253">
        <property type="entry name" value="Ribosomal_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000128469" description="30S ribosomal protein S14">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="101" mass="11579" checksum="DCBBA4F45D28EE6B" modified="2005-10-11" version="1">MAKKSSIEKNNRRRRMNRNAAAKRARLKAIIGDKTKPMEERFAATLKLAEMPRNSSATRIRNRCELTGRPRSNYRKNKLSRIALRDLGSRGLVPGLVKSSW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-02" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSV7</accession>
    <name>RS17_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsQ</name>
        <name type="ordered locus">Nwi_1373</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314648.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSV7"/>
    <dbReference type="SMR" id="Q3SSV7"/>
    <dbReference type="STRING" id="323098.Nwi_1373"/>
    <dbReference type="EnsemblBacteria" id="ABA04634">
        <property type="protein sequence ID" value="ABA04634"/>
        <property type="gene ID" value="Nwi_1373"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1373"/>
    <dbReference type="eggNOG" id="COG0186">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073626_1_1_5"/>
    <dbReference type="OMA" id="HPMYGKF"/>
    <dbReference type="OrthoDB" id="2035880at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01345_B">
        <property type="entry name" value="Ribosomal_S17_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019984">
        <property type="entry name" value="Ribosomal_S17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_S17/S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019979">
        <property type="entry name" value="Ribosomal_S17_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744">
        <property type="entry name" value="PTHR10744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00973">
        <property type="entry name" value="RIBOSOMALS17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03635">
        <property type="entry name" value="uS17_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00056">
        <property type="entry name" value="RIBOSOMAL_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000233519" description="30S ribosomal protein S17">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01345"/>
        </source>
    </evidence>
    <sequence length="82" mass="9731" checksum="67D468D6974BA661" modified="2005-10-11" version="1">MPKRTLQGVVVSDKQAKTVVVRVDRRFTHPIYKKTIRRSKNYHAHDENNEFKPGDMVWIEESKPISKLKRWTVVRGEQRKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST58</accession>
    <name>Q3ST58_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcription factor jumonji, jmjC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1272</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314561.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1272"/>
    <dbReference type="EnsemblBacteria" id="ABA04533">
        <property type="protein sequence ID" value="ABA04533"/>
        <property type="gene ID" value="Nwi_1272"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1272"/>
    <dbReference type="eggNOG" id="COG2850">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073266_0_0_5"/>
    <dbReference type="OMA" id="QNAPHRI"/>
    <dbReference type="OrthoDB" id="1885303at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13621">
        <property type="entry name" value="Cupin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="1">
        <location>
            <begin position="101"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="313" mass="35544" checksum="735FFD1E149677E4" modified="2005-10-11" version="1">MNASEPGSVITAANDALRRDFPLKPFAIRHGLAGHPLLTLPRIAQLAAELPRDLIEYNSGKVAISQDPDAIPTVDLDPVEVVKRIETAGAWMVLKRVENAPEYRRLIEDALLSVARARGFESLRDAGFEQIEGFLFVSSPNSTTPFHLDSEDNFFVQIHGEKFFTIYDNRDRRLVAEDEIERSMTKHRNLKFDESFAPRGTEFHMFGGDGCFVPYQWPHWVRTAGSHSISMAITWKTREVRRANDLHFFNSMLRGLGWPQKPPGAQPAFDAVKLAFYRTGTTAVRPLRASMTMRRILRRIALGKRANYYLKDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST59</accession>
    <name>Q3ST59_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Peptidase M50</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">Nwi_1271</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase M50B family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314560.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1271"/>
    <dbReference type="EnsemblBacteria" id="ABA04532">
        <property type="protein sequence ID" value="ABA04532"/>
        <property type="gene ID" value="Nwi_1271"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1271"/>
    <dbReference type="eggNOG" id="COG1994">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086979_0_0_5"/>
    <dbReference type="OMA" id="HEIMHGL"/>
    <dbReference type="OrthoDB" id="1401629at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06158">
        <property type="entry name" value="S2P-M50_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044537">
        <property type="entry name" value="S2P-M50-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M50" evidence="15">
        <location>
            <begin position="126"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ABA04532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="227" mass="24372" checksum="81BFA4AF1ED8DBE8" modified="2005-10-11" version="1">MPGISLYTLSVWVLPLVIAITFHEAAHGFVAYRLGDNTAWNLGRVSFNPLRHIDAFGTVILPGILLLSHAPFLFGYAKPVPVNFRNLNHPRIDMVWVALAGPATNILLALLAASAFHGLGFIPGNSAQWVADNLKNALIINVILAVFNMLPIPPLDGGRVAVGLLPSVLAAPLSRLEPYGLLILIGGLLLLPMLGRQLDLNLDVISTIVKTVTGYVIQFLLLVTGNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STA0</accession>
    <name>Q3STA0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF559 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1229</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314520.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1229"/>
    <dbReference type="EnsemblBacteria" id="ABA04491">
        <property type="protein sequence ID" value="ABA04491"/>
        <property type="gene ID" value="Nwi_1229"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1229"/>
    <dbReference type="eggNOG" id="COG2852">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107928_0_1_5"/>
    <dbReference type="OMA" id="DFYCHAA"/>
    <dbReference type="OrthoDB" id="2013215at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007569">
        <property type="entry name" value="DUF559"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04480">
        <property type="entry name" value="DUF559"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF559" evidence="2">
        <location>
            <begin position="30"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="159" mass="18008" checksum="615A6D0A268E76CD" modified="2005-10-11" version="1">MTEPRVLPSPLAGEGAERHRREAGEGACRKATARSLRAWMTDAERKLWFGLKDRRFQGLKFRRQVSVGPYIADFLCFDRRLVVEVDGGQHAESLRDADRDRWFADNGFRTLRFWNNDVLSNLEGVLTVISEGAATPHPASRLRSPPPSPARGEGKKEMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STK7</accession>
    <name>Q3STK7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flagellar basal-body rod protein FlgF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_1122</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">The basal body constitutes a major portion of the flagellar organelle and consists of five rings (E,L,P,S, and M) mounted on a central rod. The rod consists of about 26 subunits of FlgG in the distal portion, and FlgB, FlgC and FlgF are thought to build up the proximal portion of the rod with about 6 subunits each.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Bacterial flagellum basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the flagella basal body rod proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314415.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1122"/>
    <dbReference type="EnsemblBacteria" id="ABA04384">
        <property type="protein sequence ID" value="ABA04384"/>
        <property type="gene ID" value="Nwi_1122"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1122"/>
    <dbReference type="eggNOG" id="COG4786">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013687_0_0_5"/>
    <dbReference type="OMA" id="NLYFAIQ"/>
    <dbReference type="OrthoDB" id="947161at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030694">
        <property type="term" value="C:bacterial-type flagellum basal body, rod"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001444">
        <property type="entry name" value="Flag_bb_rod_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020013">
        <property type="entry name" value="Flagellar_FlgE/F/G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010930">
        <property type="entry name" value="Flg_bb/hook_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037925">
        <property type="entry name" value="FlgE/F/G-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012836">
        <property type="entry name" value="FlgF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00460">
        <property type="entry name" value="Flg_bb_rod"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06429">
        <property type="entry name" value="Flg_bbr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117143">
        <property type="entry name" value="SSF117143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03506">
        <property type="entry name" value="FlgEFG_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02490">
        <property type="entry name" value="flgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="7" id="KW-0282">Flagellum</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flg_bb_rod" evidence="5">
        <location>
            <begin position="5"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="Flg_bbr_C" evidence="6">
        <location>
            <begin position="177"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="255" mass="28063" checksum="86E58BB37ABEC837" modified="2005-10-11" version="1">MENTLLIGLSRQMMLERQMDVIANNVANVTTNGYKADHSLFNEYLMPGAHEDNFVGRDRRLSFVQDRATFHDFAGGPTEQTKNPLDVAIDGNAFLVVQTPNGERYTRDGGLQINAQGQLVTAGGHPVLGSNGPIRFQQTDRNITISDDGTVTVIEGINNATNAIRGRLRLVSFPQVQRLVKEGSNLYAAGEGNFARQDTQARINQGFIERSNVNAVTEMSRLVEVSRAYTQMATLLQQQSDLQRSAIEKLADVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STM4</accession>
    <name>Q3STM4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Flagellin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1105</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Bacterial flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial flagellin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314398.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1105"/>
    <dbReference type="EnsemblBacteria" id="ABA04367">
        <property type="protein sequence ID" value="ABA04367"/>
        <property type="gene ID" value="Nwi_1105"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1105"/>
    <dbReference type="eggNOG" id="COG1344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013955_1_0_5"/>
    <dbReference type="OMA" id="DSPVNFF"/>
    <dbReference type="OrthoDB" id="636786at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009288">
        <property type="term" value="C:bacterial-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046358">
        <property type="entry name" value="Flagellin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001029">
        <property type="entry name" value="Flagellin_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00700">
        <property type="entry name" value="Flagellin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00669">
        <property type="entry name" value="Flagellin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Flagellin_N" evidence="2">
        <location>
            <begin position="12"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Flagellin_C" evidence="3">
        <location>
            <begin position="452"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU362073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="536" mass="53566" checksum="CAA99031136198DE" modified="2005-10-11" version="1">MSDIVLSASVRQNLLSLQSTADLLARTQHRLATGKNVNTALDNPTNYFTAAALDNRANDISNLLDGIGNGVQVLQAANTGLTSLQKLVDTAKSIANQVLQSQVGYSQKSSVESLPVTGATAVDLRGTTPFTNVAATPGAALRTGTSNAAATSATLINALTDGAVATPTGPAAGESFAVNGKTITFVASGAVAADASGNITIGIDQTLDSLLGVIDVLHGNTDTASTIDGSGQIVLNTGVAHDLTLTDSTPAGVLAKLGLTAATTARGGGAGNLDGKTLTIGPTGDGVATHITFGDGANGTVRTLNDLNAQLASNHLQATINAAGVITISTANEAAAETIGAIGGTAAGAGYAFHGLVAGAPVQDPAVQASRAGLVNQYNNIIQQLNTTAADASFNGINLLGGDTLKLIFNESGKSTLTITGASMNAAGLGLASLTVGVDFLDNGSANSVIASLDSASITLRTQASTLGSNLSIVQIRQDFSKNLINVLQTGSSNLTLADTNEEAANSQALATRQSIAVSALALANQSQQSVLQLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STU3</accession>
    <name>Q3STU3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulator, Crp/Fnr family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1036</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314332.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1036"/>
    <dbReference type="EnsemblBacteria" id="ABA04298">
        <property type="protein sequence ID" value="ABA04298"/>
        <property type="gene ID" value="Nwi_1036"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1036"/>
    <dbReference type="eggNOG" id="COG0664">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075053_0_1_5"/>
    <dbReference type="OMA" id="AEVCVMP"/>
    <dbReference type="OrthoDB" id="1587662at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012318">
        <property type="entry name" value="HTH_CRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13545">
        <property type="entry name" value="HTH_Crp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00034">
        <property type="entry name" value="HTHCRP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00419">
        <property type="entry name" value="HTH_CRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="SSF51206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51063">
        <property type="entry name" value="HTH_CRP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="44"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="HTH crp-type" evidence="3">
        <location>
            <begin position="154"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="231" mass="25633" checksum="90871DF34A2FB810" modified="2005-10-11" version="1">MHTQTISAPAARILDIPHATAAEPVDPFSAIARCSGVIATEFSYKKDEEIYGEGEPSEYLYQISRGAVRTYKLLSDGRRQIGAFHLPGDILGLDPGPEHRLTAEAIVDTAVRLVPRRSIEKAATSNVHVARLLWTMTAIELRHAEDHMLLLGRKTAMERVATFLLEMDRRLAKAGMMALPMCRRDIGDYLGLTLETVSRALSYLNDQGILMFSSARQIALRNRARLSAMEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU71</accession>
    <name>Q3SU71_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Exonuclease SbcD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0908</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314211.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0908"/>
    <dbReference type="EnsemblBacteria" id="ABA04170">
        <property type="protein sequence ID" value="ABA04170"/>
        <property type="gene ID" value="Nwi_0908"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0908"/>
    <dbReference type="eggNOG" id="COG0420">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026621_1_0_5"/>
    <dbReference type="OMA" id="DWQIGKP"/>
    <dbReference type="OrthoDB" id="1831911at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00840">
        <property type="entry name" value="MPP_Mre11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041796">
        <property type="entry name" value="Mre11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014577">
        <property type="entry name" value="UCP033093_metalloPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF033093">
        <property type="entry name" value="UCP_ML1119"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="1">
        <location>
            <begin position="2"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="374" mass="40462" checksum="3016781B2B00AD75" modified="2005-10-11" version="1">MFRFIHSSDLHLGKRFGNFSGDLPSRLREARHAVIGRLAQHAREQSAATILLAGDVFDTETPASDVRRQALTEMAHHAPIRWVILPGNHDSLQATQLWTTLGAEAPDNIILAVEPRPIELAADVVLLPAPCTTRRPGRDLTEWMDSEATPEGTIRLGLAHGAIQSFSEEAVASDVIAPDRARRAGLAYLALGDWHGAMEVGPRTRYSGTPEPDRFKHDHPGEASLVSIAGPSALPEVTPLATAGFAWRTLKLHVLDGEDPASALQALLPEARLRRQTLARVMATGRSRLPARAALTRAIESATPDFSFLELDEAGLVTECEVEDLDRIDRAGALREAANALLAEAGDESRSATERETARAALARLYSYAQVVAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUC4</accession>
    <name>Q3SUC4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase IS116/IS110/IS902</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0853</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314162.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0853"/>
    <dbReference type="EnsemblBacteria" id="ABA04117">
        <property type="protein sequence ID" value="ABA04117"/>
        <property type="gene ID" value="Nwi_0853"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0853"/>
    <dbReference type="eggNOG" id="COG3547">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036902_3_3_5"/>
    <dbReference type="OMA" id="GIAHIMR"/>
    <dbReference type="OrthoDB" id="655500at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002525">
        <property type="entry name" value="Transposase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01548">
        <property type="entry name" value="DEDD_Tnp_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEDD_Tnp_IS110" evidence="1">
        <location>
            <begin position="6"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="363" mass="40690" checksum="0D965D008CBF74AB" modified="2005-10-11" version="1">MALLFCGLDVAMDETAICVVDDQGKILLETTCVSDPVAIRDVLKPYLERLRRVGHEAGSLSPWLHPEMLKLGLPAVCLETQHVRAALSAQRNKTDRTDALGIAHIMRTGWFRQAHIKSESCYRLRLLLTQRRNLKAKFLDIENTIRHSLKAFGIKLGGASRGTFDQAVREAVAQDPLTAELMDAMLTARAMLWKQYCRLHDLVVRFVAGNELCRRFMQIPGVGPVTALSFMTAIDDPSRFRRSRDVAAYFGLTSRRWQSGTSIDVQGRISKAGDPDVRRALYEAASALMTRFKRKDKVKTWGQQIAKRSCHRKACVAVARKLAVIMHAMWTDGTFYIGDPAVSAQDMAARTALKDRKLLGAHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUD4</accession>
    <name>Q3SUD4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, XRE family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0843</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314154.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04107">
        <property type="protein sequence ID" value="ABA04107"/>
        <property type="gene ID" value="Nwi_0843"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0843"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066192_29_1_5"/>
    <dbReference type="OMA" id="NEVMAIN"/>
    <dbReference type="OrthoDB" id="2063007at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="11"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="71" mass="7933" checksum="40E4025C41A8411D" modified="2005-10-11" version="1">MDLKEVMAINMRRLRYDKKLTQEELAERSGLSMRYVGSIERGAVSASVSVLGKVAVALDVDPCDLIRPPKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUD7</accession>
    <name>Q3SUD7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0840</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314152.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0840"/>
    <dbReference type="EnsemblBacteria" id="ABA04104">
        <property type="protein sequence ID" value="ABA04104"/>
        <property type="gene ID" value="Nwi_0840"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0840"/>
    <dbReference type="eggNOG" id="COG5489">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109334_1_0_5"/>
    <dbReference type="OMA" id="IWSRRNG"/>
    <dbReference type="OrthoDB" id="1730357at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007948">
        <property type="entry name" value="DUF736"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05284">
        <property type="entry name" value="DUF736"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="104" mass="11390" checksum="171E5ADC5A832938" modified="2005-10-11" version="1">MATIGTFSRTESGFSGSVKTLTLNVKSVKFVPVESDNDKGPNFRVLAGATEFGAAWKKTARETQRDYHSVKLDDPSFPAPIYASLVETDTEGEFALIWSRRAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUE8</accession>
    <name>Q3SUE8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0828</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314141.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0828"/>
    <dbReference type="EnsemblBacteria" id="ABA04093">
        <property type="protein sequence ID" value="ABA04093"/>
        <property type="gene ID" value="Nwi_0828"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0828"/>
    <dbReference type="eggNOG" id="COG1040">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1298490_0_0_5"/>
    <dbReference type="OMA" id="YLEANDK"/>
    <dbReference type="OrthoDB" id="963534at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="227" mass="25361" checksum="798D25ADCB7B6F53" modified="2005-10-11" version="1">MTTSGDTSFPSRLTKIDELTRGDHTFLEAGDECLFFGDYSARKGFSHSATNQLILNFKKPVRFRGTASWRYKALAINAAANAFTRNLGAALSQLTLVPVPPSKLKTDPEYDDRVMDMLRAIRAPAGVNPDVRELIRQTHPMAAAHESANRPPPGDWESVYAIDETLVQNEPTWIGIVDDLLVTGCRFRAMSNVLKRRFPAARITGLFLARRVPEAIDFSEFFTDLDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUF1</accession>
    <name>Q3SUF1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0825</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0825"/>
    <dbReference type="EnsemblBacteria" id="ABA04090">
        <property type="protein sequence ID" value="ABA04090"/>
        <property type="gene ID" value="Nwi_0825"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0825"/>
    <dbReference type="eggNOG" id="COG2856">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1025594_0_0_5"/>
    <dbReference type="OMA" id="DITIHRH"/>
    <dbReference type="OrthoDB" id="917043at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="248" mass="26594" checksum="56800D1CA37D6617" modified="2005-10-11" version="1">MVLAIAYPSTAAGDPHRLSAQKIASIASDIRARLFGVQPRPVDVAALIAKTARLVVNGKPINLSWGLDRPIHDENGHEALGVCENDPELPDTVMISINASLLAHQPEVIRSTAAHEFAHAIFDMPAAMGGNIRRTFRTSVAPAIARPGAPIDWVEWRADEFMGAFLVPADKLARVLPKHASALDLPFHWRAAPDGRSVPFVDLDPSEGTLGLLVDDLAEAFGVTAAFMSVRLAKGGFIGRRFETGSTL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-04-08" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUL3</accession>
    <name>COXX2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protoheme IX farnesyltransferase 2</fullName>
            <ecNumber evidence="1">2.5.1.141</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Heme B farnesyltransferase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Heme O synthase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ctaB2</name>
        <name type="ordered locus">Nwi_0763</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2E,6E)-farnesyl diphosphate + H2O + heme b = diphosphate + Fe(II)-heme o</text>
            <dbReference type="Rhea" id="RHEA:28070"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="ChEBI" id="CHEBI:60530"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.141"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; heme O biosynthesis; heme O from protoheme: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Carbon 2 of the heme B porphyrin ring is defined according to the Fischer nomenclature.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UbiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="ABA04028" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.5.1.141" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04028.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313560.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SUL3"/>
    <dbReference type="SMR" id="Q3SUL3"/>
    <dbReference type="EnsemblBacteria" id="ABA04028">
        <property type="protein sequence ID" value="ABA04028"/>
        <property type="gene ID" value="Nwi_0763"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0763"/>
    <dbReference type="HOGENOM" id="CLU_029631_0_2_5"/>
    <dbReference type="OrthoDB" id="1875497at2"/>
    <dbReference type="UniPathway" id="UPA00834">
        <property type="reaction ID" value="UER00712"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008495">
        <property type="term" value="F:protoheme IX farnesyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048034">
        <property type="term" value="P:heme O biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13957">
        <property type="entry name" value="PT_UbiA_Cox10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00154">
        <property type="entry name" value="CyoE_CtaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006369">
        <property type="entry name" value="Protohaem_IX_farnesylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030470">
        <property type="entry name" value="UbiA_prenylTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43448">
        <property type="entry name" value="PTHR43448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01473">
        <property type="entry name" value="cyoE_ctaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00943">
        <property type="entry name" value="UBIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0350">Heme biosynthesis</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000327098" description="Protoheme IX farnesyltransferase 2">
        <location>
            <begin position="1"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="312" mass="33564" checksum="52D442003D86F105" modified="2008-04-08" version="2">MSVVDQKAVELPPRISEAGVADYFALLKPRVMSLVIFTALVGLMIAPGHIHPVLGFIAILCIAVGAGASGALNMALEGDIDVLMSRTANRPIPRGRITRGEAMGFGLTLSFFSVMTLGALVNWYAGGLLAFTIFFYVVIYTMGLKRRTAQNIVIGGAAGALPPVVAWAAATGSLSVEPLLLFLIIFFWTPPHFWALALFRSDDYARAGVPMLPVVAGPDATRLQILLYTIVLVAIAAAPWPLGYFDWVYGVTSLILGAGMLVLAIEVYRHRTGSQALRATRRLFAFSILYLFALFAVLLLDVVAKAVAPLIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUQ8</accession>
    <name>Q3SUQ8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">G8 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0717</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314037.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0717"/>
    <dbReference type="EnsemblBacteria" id="ABA03983">
        <property type="protein sequence ID" value="ABA03983"/>
        <property type="gene ID" value="Nwi_0717"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0717"/>
    <dbReference type="eggNOG" id="COG4990">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_250951_0_0_5"/>
    <dbReference type="OMA" id="NESMTRW"/>
    <dbReference type="OrthoDB" id="222701at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019316">
        <property type="entry name" value="G8_domain"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51484">
        <property type="entry name" value="G8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G8" evidence="2">
        <location>
            <begin position="119"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1457" mass="156410" checksum="2F72A2FC139CF19F" modified="2005-10-11" version="1">MHIDHEGNRVGSRIAVRSRMTAGLRIAGSCGVLLFFGLSTSPALSAKTKAAAQQNTTVTCSSGFLPAAGSTNSPPDLVVNGECHVKPDQTYYFENVNVLDKGQLVFDELDKTIKPTATHFWAQSMVIENGGTVVAGSQRQPYGNYGGVLTIHLYGADRNASGPALGALCRTAQNTDTGPCGIPTSAWRDNGKTKLALPGGVSDFFYRYGPLHGDGKCSDGSVWSSTSPCDDSQDKQVGYFGYKVLAVSYGGSLELWGAKGSCNSMGCSNPQLHPSWTRLDRSLAAGGGAGPNDPLVVAGASNLRPENLHVQAGDQIVVTTTDYLPGHSETFVVKDAHTGDNGRGRWLTKIRVDHGAAWPHNGERYALTDKLAPSQGRLDLDPDLVKNGVETRAAVAILNRSIRIVSGGDRPGEEFPDSTPEKPCVAQDGKGPCYSLGGHVVFRQGFKAVHVKGVEFKELGQGGRMAHYPVHFHMARQTPEGTYVQDSSINESMTRWIVLHSTQGVMIAGNVGYKSIGHGFYLEDGTETDNMFYGNLGILARAAVANEQNPRSIPGILAANEDKKPVPKFPFLTDSAYPSVFWITNGWNEFIGNMAAGATACGSAYWFVPVWNSDRPDVMTPNNSLDQRHMKWSGYAGLQKDGAYRGSTPLKAFVGNFATSTMMSFQTTGDAPECSGVLKPASRAAQDKTADAAQGGAATNALVAVRSLAPDPNDNEADDHYYPHAVGGNRHATACNRKDDGTYDCEGIKFCDPGHPENCAVTVIDKFTSMFHWAQHNVAAIWLRNQWYLVVNSVLSDVQNGGLTFITGGDYTHASVIRGYWALARKSVFIGHSQPQDDAHAFALDAGPFNARSKLRCDWQVAGLSPSNYCLSAKEGVSIPLINFDSGQRLFNIYDGPAYQDSNAYLDITKSDCPVTGSKTDPGCMYGSGVVLGVTKDPLNGQCYLPNAAIGWKQNNGFFYPPAFHSANLFFSNVDIRHFVFDPLFKAPDGVAEKQDFGQGGTYLTATSGPNAVSNFYCTSTDNMFDGWSGIDRQTELTDDDGTLTGLISSKPGGETVSINEDQFFTAKLETSECKSNLRVSPSLVCPQKPLPPASTPSTAKTSPYDYVATAIAPGCSEKSPPDPSKPYGRCGDTPDLGQGGDWSSECSNGACYGVPLYRQYLTTTELGHWQTNGCAANANTPACRWPFMRMGGQNMYQRETLTVNNGVYYVDTSVSKTTQNTENYTKMPQTRDVNVFAPGQTYYMFFLYAKPSTAQTYQIYVGEGFDTGTVKPVRGSLATAPIKFSPDSAPNAGKWFTVKGVSKGVLTIETNFAAQSELDPRQQKNGLCGPATFCGWKADGSCGSKLASADPGKEPLLLADPALGKEADAVCRTWAVKDLDCPAAGCFGFAFTLPANFKAQDQGQAARPTPAAFPTTGEFATRFLNTKIVPDNASHARDGVETECFYPRLPGSCPAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUT8</accession>
    <name>Q3SUT8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0687</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314009.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0687"/>
    <dbReference type="EnsemblBacteria" id="ABA03953">
        <property type="protein sequence ID" value="ABA03953"/>
        <property type="gene ID" value="Nwi_0687"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0687"/>
    <dbReference type="eggNOG" id="COG0791">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_414284_0_0_5"/>
    <dbReference type="OMA" id="CADLPYF"/>
    <dbReference type="OrthoDB" id="274618at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229281" evidence="2">
        <location>
            <begin position="27"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="249"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="623"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="626"/>
            <end position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="659" mass="72907" checksum="F0F566FED01FC13C" modified="2005-10-11" version="1">MRLTSRFAAGLVAAFILAAIGPPAEAAARPCEQAVGFSVLPSPVAPWKGAPLRVMITTEKPLQGVISLVAPDGRVVARSSDRHGSAPYSWFAEAAQPTAGVWRATLTLDSAMADCSTITRDISVSARKPNPPPIPSGAFWQVRNSWDHTTESLFSAWIEKLFDAPPDQDLSWKAWHEVLHNRSRNFLFNYLGRGEDDKRTGLRPDCADFVYFLRAYFAYKMGLPFGYSNCSRGARGKAPRCYQWFDMEHPEKTRPQPPSEQETASAAAPGAMPPGQAPALLQPFASLQPAPAETLVAPPKARPKRPTTFNEYLRDVGDVVHTGAVRAAASDDNTDFYTVALTQEALRPGTVYADPYGHVLMLVHRVPEKNGKPGVFLAVDAEPDGSITRKRFWRGNFLFAHDPALGSPGFKRFRPVVRGKGGALRRLVNAEITSNTQYADFSLDQSEMSAEDFYDRMDDVMSPEPLDPVKAMTDAIESLNEQVKTRVTAVENGRQYQNKHAGEVAMPNGSAIFGTTGAWEDYSTPARDFRLLIAIDVVRGFPDRVIRRATRYAMPGGKSIAEVKSELQGVLASELASRKISYMRSDGSQWTLSLKEVVERAPRFEMAYNPNDCAEVRWGASEGSEEASTCKRHAPQAQREKMSSKYRTWFHERNWPTNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUZ8</accession>
    <name>Q3SUZ8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Autoinducer synthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0626</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the autoinducer synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313953.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0626"/>
    <dbReference type="EnsemblBacteria" id="ABA03893">
        <property type="protein sequence ID" value="ABA03893"/>
        <property type="gene ID" value="Nwi_0626"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0626"/>
    <dbReference type="eggNOG" id="COG3916">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085711_3_0_5"/>
    <dbReference type="OMA" id="IWEWTRY"/>
    <dbReference type="OrthoDB" id="1725271at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009372">
        <property type="term" value="P:quorum sensing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001690">
        <property type="entry name" value="Autoind_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39322">
        <property type="entry name" value="PTHR39322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00765">
        <property type="entry name" value="Autoind_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01549">
        <property type="entry name" value="AUTOINDCRSYN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51187">
        <property type="entry name" value="AUTOINDUCER_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0071">Autoinducer synthesis</keyword>
    <keyword evidence="2" id="KW-0673">Quorum sensing</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="210" mass="24312" checksum="4C5A4C6C54B048B1" modified="2005-10-11" version="1">MIHIVTAENIGSYQYEMEQAYRLRHNVFVDEMGWDDLRKPDGREIDQFDDGRALHMLYIEEDRVLGYQRMLPSMRPHLLTEVLPHLCEGDFPVGPHIWEWTRYCVTRQHRDRGRILSPVGNLLLSAIVEWGLASGVQKIIIEMNPLWLLRLVQLNFRVTPLGLPQQIGKDSIIAVTAAFDRRTLKKLQEVRGNIVPAIVAQPEADQRLRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV98</accession>
    <name>Q3SV98_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Flagellum-specific ATP synthase</fullName>
            <ecNumber evidence="4">7.1.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Nwi_0526</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probable catalytic subunit of a protein translocase for flagellum-specific export, or a proton translocase involved in local circuits at the flagellum.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313854.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0526"/>
    <dbReference type="EnsemblBacteria" id="ABA03793">
        <property type="protein sequence ID" value="ABA03793"/>
        <property type="gene ID" value="Nwi_0526"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0526"/>
    <dbReference type="eggNOG" id="COG1157">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022398_5_1_5"/>
    <dbReference type="OMA" id="MDSATRF"/>
    <dbReference type="OrthoDB" id="875807at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009288">
        <property type="term" value="C:bacterial-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030257">
        <property type="term" value="C:type III protein secretion system complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044781">
        <property type="term" value="P:bacterial-type flagellum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030254">
        <property type="term" value="P:protein secretion by the type III secretion system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005714">
        <property type="entry name" value="ATPase_T3SS_FliI/YscN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022426">
        <property type="entry name" value="FliI_clade3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040627">
        <property type="entry name" value="T3SS_ATPase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18269">
        <property type="entry name" value="T3SS_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03498">
        <property type="entry name" value="FliI_clade3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01026">
        <property type="entry name" value="fliI_yscN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword evidence="10" id="KW-1006">Bacterial flagellum protein export</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AAA" evidence="12">
        <location>
            <begin position="156"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="109"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA03793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="441" mass="47477" checksum="D04C83601AC619E5" modified="2005-10-11" version="1">MKGLAGRIGEIERVNIYGRVVGVRGLMVEIAGPIHAMSVGARIVIETGVGRTIPAEVVGFNGDNAVVMPFASLDGVRRGCRALVANASDSVRPAAAWLGRVLNALGEPIDGKGPLPQGPTPVPYRNSPPSAHSRQRVGQPLDLKVRALNTFLTCCRGQRLGIFAGSGVGKSVLLSMLARNADADVSVIGLIGERGREVQEFLQDDLGDEGLARSVVVVATSDEPALMRRQAAYLTLAIAEFFRDEGRDVMCMMDSVTRFAMAQREIGLSTGEPPTTKGYTPTVFTELPRLLERAGPGADKGTITGIFTVLVDGDDHNEPVADAVRGILDGHIVMERAIAERGRYPAINILKSVSRTMPRSADPAFLPDITCARRMMATYSDMEELIRLGAYRAGSNAEVDEAIRLNPVLEDFLRQAKDEVTSIEDGYRRLQQIVNTLATES</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVA1</accession>
    <name>CHEB_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein-glutamate methylesterase/protein-glutamine glutaminase</fullName>
            <ecNumber evidence="1">3.1.1.61</ecNumber>
            <ecNumber evidence="1">3.5.1.44</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cheB</name>
        <name type="ordered locus">Nwi_0523</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in chemotaxis. Part of a chemotaxis signal transduction system that modulates chemotaxis in response to various stimuli. Catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins or MCP) by CheR. Also mediates the irreversible deamidation of specific glutamine residues to glutamic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-L-glutamate 5-O-methyl ester + H2O = H(+) + L-glutamyl-[protein] + methanol</text>
            <dbReference type="Rhea" id="RHEA:23236"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:10311"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:82795"/>
            <dbReference type="EC" id="3.1.1.61"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-glutaminyl-[protein] = L-glutamyl-[protein] + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16441"/>
            <dbReference type="Rhea" id="RHEA-COMP:10207"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="EC" id="3.5.1.44"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Contains a C-terminal catalytic domain, and an N-terminal region which modulates catalytic activity.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Phosphorylated by CheA. Phosphorylation of the N-terminal regulatory domain activates the methylesterase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CheB family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.61" evidence="1"/>
    <dbReference type="EC" id="3.5.1.44" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVA1"/>
    <dbReference type="SMR" id="Q3SVA1"/>
    <dbReference type="STRING" id="323098.Nwi_0523"/>
    <dbReference type="EnsemblBacteria" id="ABA03790">
        <property type="protein sequence ID" value="ABA03790"/>
        <property type="gene ID" value="Nwi_0523"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0523"/>
    <dbReference type="eggNOG" id="COG2201">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_51_0_5"/>
    <dbReference type="OMA" id="MLEMHRA"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000156">
        <property type="term" value="F:phosphorelay response regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008984">
        <property type="term" value="F:protein-glutamate methylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050568">
        <property type="term" value="F:protein-glutamine glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018277">
        <property type="term" value="P:protein deamination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006482">
        <property type="term" value="P:protein demethylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16432">
        <property type="entry name" value="CheB_Rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00099">
        <property type="entry name" value="CheB_chemtxs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008248">
        <property type="entry name" value="CheB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035909">
        <property type="entry name" value="CheB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000673">
        <property type="entry name" value="Sig_transdc_resp-reg_Me-estase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01339">
        <property type="entry name" value="CheB_methylest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52738">
        <property type="entry name" value="SSF52738"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50122">
        <property type="entry name" value="CHEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0145">Chemotaxis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000225464" description="Protein-glutamate methylesterase/protein-glutamine glutaminase">
        <location>
            <begin position="1"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="1">
        <location>
            <begin position="22"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="CheB-type methylesterase" evidence="1">
        <location>
            <begin position="221"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="150"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="206"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="269"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="365"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="425" mass="45007" checksum="4FF00D2EF3A212D0" modified="2005-10-11" version="1">MGMSTAVASPVALDSGKLEPLRVMVVDDSVVIRGLISRWIEAEPDMMVAASLRTGRDAVSQVERADPDVVVLDIEMPELDGISALPQLLAKKRNLIVIMASTLTRRNAEISFKALSLGASDYIPKPESTREVAAADIFRHDLMQKIRHLAAKRRRPATVASPPPDHDDYGSNASTIMNAVDSNISERDAGGKPRRTFPHPALVQREQQPRSAQAARAMSRPQPTLRSFSAHLPRALLIGSSTGGPQALMTLVAGIGPVIDRCPVLITQHMPPTFTTILAEHLARAAGRPAHEGVDQEIVKQGHIYLAPGGRHMRVARKGADAVIVLDNGPAVNFCKPAVDPLFMSAIDVWQGGALAVILTGMGSDGMRGGTQIVAAGGSIIAQDEASSVVWGMPGAAVQAGICAAVLPLQQIAPKLVRLFAGDGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVA2</accession>
    <name>Q3SVA2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CheY protein</fullName>
            <ecNumber evidence="3">3.1.1.61</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0522</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.1.61" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313850.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0522"/>
    <dbReference type="EnsemblBacteria" id="ABA03789">
        <property type="protein sequence ID" value="ABA03789"/>
        <property type="gene ID" value="Nwi_0522"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0522"/>
    <dbReference type="eggNOG" id="COG3706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_12_5"/>
    <dbReference type="OMA" id="IMKPFDG"/>
    <dbReference type="OrthoDB" id="1630092at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008984">
        <property type="term" value="F:protein-glutamate methylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="3"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="121" mass="13696" checksum="B0300737425D1B27" modified="2005-10-11" version="1">MKTCLVVDDSSVIRKVARRILEGFNFRIVEAENGEKGLEACRLELPDAVLLDWNMPVMDGYEFLRNLRRMPGGDQPKVVFCTTENDVAHIARALHAGANEYIMKPFDKDIVTAKFQEIGLI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-10-13" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVI2</accession>
    <name>OBG_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GTPase Obg</fullName>
            <ecNumber evidence="1">3.6.5.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">GTP-binding protein Obg</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">obg</name>
        <name type="ordered locus">Nwi_0442</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. Plays a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313773.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVI2"/>
    <dbReference type="SMR" id="Q3SVI2"/>
    <dbReference type="STRING" id="323098.Nwi_0442"/>
    <dbReference type="EnsemblBacteria" id="ABA03709">
        <property type="protein sequence ID" value="ABA03709"/>
        <property type="gene ID" value="Nwi_0442"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0442"/>
    <dbReference type="eggNOG" id="COG0536">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011747_2_0_5"/>
    <dbReference type="OMA" id="VVFDWEP"/>
    <dbReference type="OrthoDB" id="603226at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01898">
        <property type="entry name" value="Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.210.12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01454">
        <property type="entry name" value="GTPase_Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014100">
        <property type="entry name" value="GTP-bd_Obg/CgtA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006074">
        <property type="entry name" value="GTP1-OBG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006169">
        <property type="entry name" value="GTP1_OBG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036726">
        <property type="entry name" value="GTP1_OBG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045086">
        <property type="entry name" value="OBG_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11702">
        <property type="entry name" value="PTHR11702"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01018">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002401">
        <property type="entry name" value="GTP_bd_Obg/CgtA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82051">
        <property type="entry name" value="SSF82051"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02729">
        <property type="entry name" value="Obg_CgtA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00905">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51883">
        <property type="entry name" value="OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000386090" description="GTPase Obg">
        <location>
            <begin position="1"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Obg" evidence="2">
        <location>
            <begin position="1"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="OBG-type G" evidence="1">
        <location>
            <begin position="160"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="335"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="166"/>
            <end position="173"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="191"/>
            <end position="195"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="212"/>
            <end position="215"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="279"/>
            <end position="282"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="308"/>
            <end position="310"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="358" mass="38264" checksum="7228061F26E64D3C" modified="2005-10-11" version="1">MKFLDEAKVYIRSGDGGNGCVAFRREKFIEFGGPSGGNGGRGGDVIVEVADGLNTLIDYRYQQHFKAPKGANGMGSDRHGANGKAIVLKVPLGTQIIDEDRETLIHDFTRVGERLVLAEGGNGGFGNAHFKSSTNRAPRRANPGQPGEERWIWLRLKLIADAGLVGLPNAGKSTFLSKVSAAKPKIADYPFTTLHPQLGVVRVQDREFVLADIPGLIEGAHEGAGLGDRFLGHVERCRVLLHLVDAGCDHAGRAYKIVRGEMEAYAGALAEKVEIVALNKIDAVTAEDLKKQRDRLKRAAKKTPLLVSGVTGEGVQDVLRALVEVIGQAPVSDKAKGADASAAQAMETPVARAKPWSP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-26" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVJ8</accession>
    <name>XERC_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tyrosine recombinase XerC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">xerC</name>
        <name type="ordered locus">Nwi_0426</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules. The XerC-XerD complex is essential to convert dimers of the bacterial chromosome into monomers to permit their segregation at cell division. It also contributes to the segregational stability of plasmids.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a cyclic heterotetrameric complex composed of two molecules of XerC and two molecules of XerD.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'phage' integrase family. XerC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313757.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVJ8"/>
    <dbReference type="SMR" id="Q3SVJ8"/>
    <dbReference type="STRING" id="323098.Nwi_0426"/>
    <dbReference type="EnsemblBacteria" id="ABA03693">
        <property type="protein sequence ID" value="ABA03693"/>
        <property type="gene ID" value="Nwi_0426"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0426"/>
    <dbReference type="eggNOG" id="COG4974">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027562_9_0_5"/>
    <dbReference type="OMA" id="QVYAHIG"/>
    <dbReference type="OrthoDB" id="745068at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009037">
        <property type="term" value="F:tyrosine-based site-specific recombinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01808">
        <property type="entry name" value="Recomb_XerC_XerD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004107">
        <property type="entry name" value="Integrase_SAM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023009">
        <property type="entry name" value="Tyrosine_recombinase_XerC/XerD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02899">
        <property type="entry name" value="Phage_int_SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0159">Chromosome partition</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0229">DNA integration</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000073667" description="Tyrosine recombinase XerC">
        <location>
            <begin position="1"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Core-binding (CB)" evidence="3">
        <location>
            <begin position="16"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="2">
        <location>
            <begin position="128"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="270"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="293"/>
        </location>
    </feature>
    <feature type="active site" description="O-(3'-phospho-DNA)-tyrosine intermediate" evidence="1">
        <location>
            <position position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <sequence length="321" mass="34998" checksum="811B8F5495C1E163" modified="2005-10-11" version="1">MAKRAEPLPDPACAASDIGQQIVRWLTHLRAERRLSPKTLEAYARDVRQCLMFLGQHWSEQVTLEGFVALEPADIRSFMAARRADDIGGRSLMRSLAGLRSFARFLEREGLGKVGALGAIRAPKVGKSVPKPIHMSAAKRFADASERAGEDREPWIWARDAAVMALLYGSGLRISEALGLKRRDVPPPGAGDVLVVTGKGNKTRMVPVLQNVLALIHEYVAMCPHSLPPEGPIFVGARGGPLRARIIQLVMARMRGALGLPDSATPHALRHSFATHLLSRGGDLRAIQELLGHASLSTTQIYTGIDSERLLDVYRTAHPRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVM3</accession>
    <name>Q3SVM3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Heat shock protein DnaJ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0401</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0401"/>
    <dbReference type="EnsemblBacteria" id="ABA03668">
        <property type="protein sequence ID" value="ABA03668"/>
        <property type="gene ID" value="Nwi_0401"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0401"/>
    <dbReference type="eggNOG" id="COG2214">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096103_0_0_5"/>
    <dbReference type="OMA" id="VLHPDMN"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="SSF46565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="173"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="232" mass="26098" checksum="5E48E31122077E52" modified="2005-10-11" version="1">MRCQDLKPGALRCQRQIMPIDTSKFFDSIRIKPRAGAKQPRMREREEVIACEWPDCQSRGPHRAPKGRGNEGGYFHFCLDHVRAYNQSYNFFQGMNAEAVARYQKDALTGHRPTWKMGANGAKGKGRADAIDAEGVADPFSVFGELNGRGKWRSGPQFESKPETRKIFNAERKALQVLGLGADATLETVKAKYKMLVKQHHPDANGGDRSTEDRLIEIIKAYNYLKTVVRGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVS0</accession>
    <name>Q3SVS0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0354</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313686.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0354"/>
    <dbReference type="EnsemblBacteria" id="ABA03621">
        <property type="protein sequence ID" value="ABA03621"/>
        <property type="gene ID" value="Nwi_0354"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0354"/>
    <dbReference type="HOGENOM" id="CLU_2918015_0_0_5"/>
    <dbReference type="OrthoDB" id="2010269at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="61" mass="6806" checksum="18C0BD7732372D2F" modified="2005-10-11" version="1">MRKLIAFDDETMTALTQLGHDRMATLQDLADEAFADLLKKHAGRSISRTLCAKARASENRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVS7</accession>
    <name>Q3SVS7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_0347</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0347"/>
    <dbReference type="EnsemblBacteria" id="ABA03614">
        <property type="protein sequence ID" value="ABA03614"/>
        <property type="gene ID" value="Nwi_0347"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0347"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_6_5"/>
    <dbReference type="OMA" id="VIWAENI"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025908">
        <property type="entry name" value="Sensor_TM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025919">
        <property type="entry name" value="Stimulus_sens_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13755">
        <property type="entry name" value="Sensor_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13756">
        <property type="entry name" value="Stimulus_sens_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="300"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="9">
        <location>
            <begin position="321"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="384"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA03614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="611" mass="67854" checksum="D1ADC9813E5827BF" modified="2005-10-11" version="1">MLDRTQPDLDPDAEDTSQVLASESPADAESPVDQVERTPYWRRPLEWLRRAGQFLFTLSFSSLTRRIVSLNLAGLIALVASILYLSQFRAGLIDARAQSLLVQGEIIASAIAASATVETNTITIDPDRLLDLKPGESYGAPDEISGLDFPINPERVAPVLRRLISPTKTRARIYDRDGVLILDSRSLFRRGDVIRFELPPPTDVKPGFIERTTIAIRTWLNRGDLPLYRELGPENGNGYQEVAQSLRGEKSSMVRINDRGEIIVSVAVPVERPRSTIFGSLMLSTQGDDIDQMVMAERLAILKVFGVAMIVMIVLSLLLASTIAGPVRRLADGAERVRRRIKTRIEIPDFTRRRDEIGHLSGALRDMTDALYNRIEAIETFAADVSHELKNPLTSLRSAVETLPLAKTEASRGRLLEVIDHDVRRLDRLISDISDASRLDAELQRQDEAPVDIRRLLTTLTSVANETRLGRDISVDVRFEGGASDGFSVPGHDLRLGQVISNLLTNAQSFSKPGGRVRILCRRLKSEIEIVVDDDGPGIRPDALERVFERFYTDRPHQGFGQNSGLGLSISKQIIEAHRGRIWAENRPGPTDTHGDTRIAGARFIVRLPAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVX4</accession>
    <name>Q3SVX4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Pilus assembly protein CpaD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0300</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313633.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0300"/>
    <dbReference type="EnsemblBacteria" id="ABA03567">
        <property type="protein sequence ID" value="ABA03567"/>
        <property type="gene ID" value="Nwi_0300"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0300"/>
    <dbReference type="eggNOG" id="COG5461">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098988_0_0_5"/>
    <dbReference type="OMA" id="HPIAIQE"/>
    <dbReference type="OrthoDB" id="1561283at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019027">
        <property type="entry name" value="Pilus_biogenesis_CpaD-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013361">
        <property type="entry name" value="Pilus_CpaD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09476">
        <property type="entry name" value="Pilus_CpaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02522">
        <property type="entry name" value="pilus_cpaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229304" evidence="2">
        <location>
            <begin position="28"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="246" mass="26470" checksum="C485E56C08295F25" modified="2005-10-11" version="1">MTSADSAARRNLRLAILVAAGLSAALGACTHTARVDTTGSIPDDYRLRHPIAIQEADRTVNIFIGNTRGGLTAPQRADVVGLASVWLREGTGAIVAEVPTGTGNARAAADSFREVRSLLAAAGVPPRGIIVRHYHPADPRLFATIRLTYPRIAAVAGPCGVWPDDIGPSVKNRGYLDNKPYWNFGCATQRNLASMIDNPSDLVQPRPETPAYTARRTQSFEKYRKGMTTTTDYPEAEKAKLSDTGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW19</accession>
    <name>Q3SW19_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Antirestriction protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0255</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313588.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0255"/>
    <dbReference type="EnsemblBacteria" id="ABA03522">
        <property type="protein sequence ID" value="ABA03522"/>
        <property type="gene ID" value="Nwi_0255"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0255"/>
    <dbReference type="eggNOG" id="COG4227">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041111_0_0_5"/>
    <dbReference type="OMA" id="APIWMTF"/>
    <dbReference type="OrthoDB" id="1509644at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017113">
        <property type="entry name" value="Antirestriction_ArdC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013610">
        <property type="entry name" value="ArdC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041459">
        <property type="entry name" value="MPTase-PolyVal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08401">
        <property type="entry name" value="ArdcN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18818">
        <property type="entry name" value="MPTase-PolyVal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037112">
        <property type="entry name" value="Antirestriction_ArdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ArdcN" evidence="1">
        <location>
            <begin position="4"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="MPTase-PolyVal" evidence="2">
        <location>
            <begin position="151"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="299" mass="33406" checksum="5AEE3651F1B0F8D8" modified="2005-10-11" version="1">MKSDVYTRITDQIVAELEKGVRPWHKPWNAGHVAGPISRPLRSCGKPYSGINIVMLWATAMERGYSAPIWMTFRQALELGAHVRKGEKGALVVFASKITRTEQDDSGQAHEREIPFLKGYTVFNVEQIDDLPAHYTASTAPRLNAEQRIARAEGFFAATRADIRHGGNSAHYVPSLDYIQMPPFESFRDAAAYYATLAHECTHWTRHEKRLDRDLGRKRFGDQGYAMEELVAELGAAFLCADLDLVPQIRDDHAPYIAGWLKVLKDDKRAIFTAASHAQRAADFLTGLQPVVAQTAEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWB9</accession>
    <name>Q3SWB9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nuclease (SNase-like protein)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0154</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0154"/>
    <dbReference type="EnsemblBacteria" id="ABA03422">
        <property type="protein sequence ID" value="ABA03422"/>
        <property type="gene ID" value="Nwi_0154"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0154"/>
    <dbReference type="eggNOG" id="COG1525">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122388_0_0_5"/>
    <dbReference type="OMA" id="NIGPDKY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016071">
        <property type="entry name" value="Staphylococal_nuclease_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00565">
        <property type="entry name" value="SNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00318">
        <property type="entry name" value="SNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50199">
        <property type="entry name" value="SSF50199"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="TNase-like" evidence="2">
        <location>
            <begin position="69"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="190" mass="20633" checksum="C8B35FBAF365F930" modified="2005-10-11" version="1">MYPYSRINAWSGPRRRNLLSPVLPWIFIVGVAAGAMLPIRSWIDAPRAVNPRTPTEARAIWARAADAAGRHQADVIRTIDGDTFEALVHLWPGLDMTTRVRLRGIDTAEMKASCAEEFRLAKAASARLRDLLGEGGVTIANIGPDKYQGRVVADAATARTPDVSAAMLASGLARVYHGGRRESWCNGASR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWF0</accession>
    <name>HIS4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase</fullName>
            <ecNumber evidence="1">5.3.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisA</name>
        <name type="ordered locus">Nwi_0123</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide = 5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:15469"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="5.3.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 4/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HisA/HisF family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.16" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWF0"/>
    <dbReference type="SMR" id="Q3SWF0"/>
    <dbReference type="STRING" id="323098.Nwi_0123"/>
    <dbReference type="EnsemblBacteria" id="ABA03391">
        <property type="protein sequence ID" value="ABA03391"/>
        <property type="gene ID" value="Nwi_0123"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0123"/>
    <dbReference type="eggNOG" id="COG0106">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048577_1_1_5"/>
    <dbReference type="OMA" id="EWLHLVD"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00009"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003949">
        <property type="term" value="F:1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04732">
        <property type="entry name" value="HisA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01014">
        <property type="entry name" value="HisA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006063">
        <property type="entry name" value="HisA_bact_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044524">
        <property type="entry name" value="Isoase_HisA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023016">
        <property type="entry name" value="Isoase_HisA-like_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090">
        <property type="entry name" value="PTHR43090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00007">
        <property type="entry name" value="TIGR00007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000229063" description="1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase">
        <location>
            <begin position="1"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01014"/>
        </source>
    </evidence>
    <sequence length="249" mass="26115" checksum="69F9F97C665534A5" modified="2005-10-11" version="1">METVILFPAIDLKSGQCVRLEQGDMARATVFNLDPVEQARAFAAQGFEYLHVVDLDGAFAGKPVNADAVERVLRNVALPMQLGGGIRDLKTVEAWLGKGVARVIIGTAAVRDPVLVREAAKAFPGRVAVGLDARDGKVAVEGWAETSEVTALDIARRFEDAGIAAIIFTDIARDGLLKGLNLDATIALADSISIPVIASGGFASIADVKALLEPRAKKLAGAISGRALYDGRLDPVEALKLIHAARAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWH4</accession>
    <name>Q3SWH4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thioredox_DsbH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0099</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313436.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0099"/>
    <dbReference type="EnsemblBacteria" id="ABA03367">
        <property type="protein sequence ID" value="ABA03367"/>
        <property type="gene ID" value="Nwi_0099"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0099"/>
    <dbReference type="eggNOG" id="COG1331">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014051_4_2_5"/>
    <dbReference type="OMA" id="PFYFGTY"/>
    <dbReference type="OrthoDB" id="854580at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02955">
        <property type="entry name" value="SSP411"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004879">
        <property type="entry name" value="DUF255"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024705">
        <property type="entry name" value="Ssp411"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42899">
        <property type="entry name" value="PTHR42899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03190">
        <property type="entry name" value="Thioredox_DsbH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006402">
        <property type="entry name" value="UCP006402_thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredox_DsbH" evidence="1">
        <location>
            <begin position="13"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="676" mass="74679" checksum="AF768227EAE8D68F" modified="2005-10-11" version="1">MNQAAGSSGRLANRLSAETSPYLLQHQHNPVDWWPWGPEALAEAQRSNRPILLSIGYAACHWCHVMAHESFEDDDVAAVMNELFVCIKVDREERPDIDQIYMSALHHLGEQGGWPLTMFLSPDGSPFWGGTYFPKLPDFGRPAFTDVLQSVARVFRDQPDQIARHRDTLIARLSERATTKSPANLGVAELNNAAVAIMRSTDPVNGGLRGAPKFPQCSVLELLWRAGARTRDDRFFAATTLTLTRMSQGGIYDHIGGGYARYSVDDRWLVPHFEKMLYDNAQILDLLALDYARSKNPLYRERAIETVDWLRREMLTAEGGFASSLDADSEGEEGRFYVWSLSEIDDVLGAADAADFAARYDITANGNFERRNIPNRLKSIDVANDDSAHMRALRKKLLVRRESRVRPGLDDKILADWNGLMIAALVHGACVFDKPDWLRIARAAYDFIRTMMTRDGRLGHSWREGRLLIPALASDYATMARAALALFEATGDGTFLEQALRWQSTLDTHYADAAHGGYYLTADDAEGLIVRPHSSEDDAIPNHDGVIAQNLVRLAALTGDAKWRDRIDSHFAALLPRATEKGFGQLSLMNALDLRLTGAEIVVAGEDAQAAALLGAARKLPYATSIVLHAPHADALPADHPARAKLSAVAQSAAFICRGQSCSLPVTQPDALNELM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWI2</accession>
    <name>SYL_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Leucine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Leucyl-tRNA synthetase</fullName>
            <shortName evidence="1">LeuRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">leuS</name>
        <name type="ordered locus">Nwi_0091</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313428.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWI2"/>
    <dbReference type="SMR" id="Q3SWI2"/>
    <dbReference type="STRING" id="323098.Nwi_0091"/>
    <dbReference type="PRIDE" id="Q3SWI2"/>
    <dbReference type="EnsemblBacteria" id="ABA03359">
        <property type="protein sequence ID" value="ABA03359"/>
        <property type="gene ID" value="Nwi_0091"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0091"/>
    <dbReference type="eggNOG" id="COG0495">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004427_0_0_5"/>
    <dbReference type="OMA" id="TFMVLAP"/>
    <dbReference type="OrthoDB" id="32262at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00049_B">
        <property type="entry name" value="Leu_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002302">
        <property type="entry name" value="Leu-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025709">
        <property type="entry name" value="Leu_tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43740">
        <property type="entry name" value="PTHR43740"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13603">
        <property type="entry name" value="tRNA-synt_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00985">
        <property type="entry name" value="TRNASYNTHLEU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="SSF50677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00396">
        <property type="entry name" value="leuS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000009383" description="Leucine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="630"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="633"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00049"/>
        </source>
    </evidence>
    <sequence length="883" mass="98985" checksum="61369988A74A46CD" modified="2005-10-11" version="1">MTSERYNARDAEPRWQAEWDRQAIFATKNDDPREKYYVLEMFPYPSGRIHIGHVRNYTLGDVIARYMRARGYNVLHPMGWDAFGLPAENAAIERKIAPKAWTYDNIKAMKKQLRSIGLSLDWSREIATCDPAYYKHQQKMFLDFLRAGLAEREKRKINWDPVDMTVLANEQVIDGRGWRSGAVVEQREMNQWVFKITKFSQELLDALGTLDRWPDKVRLMQRNWIGRSEGLLIRFALDATTTPNNESELKIFTTRPDTLFGARFMAIAPDHPLAQAAAKDNPALAGFIAECKQRGTAQAEIDTAEKMGFDTGIRAIHPFDAAWALPVYVANFILMEYGTGAIFGCPAHDQRDLDFVNKYGLGNTPVVCPEGQDPASFVITDTAYDGDGRMINSRFLDGMSADAAKEEVAKRLEGETRGNEPVAERKVNFRLRDWGISRQRYWGCPIPVIHCPNCDVVPVPEKDLPVTLPEDVTFDRPGNALDHHPTWKHVDCPKCGGKATRETDTMDTFVDSSWYFARFTDPWNDSAPTTREVADRMLPVDQYIGGVEHAILHLLYSRFFTRAMKATGHLGMDEPFKGMFTQGMVVHETYRKPDGGWASPEEVRIEVDGNNRRATLITTGEPVEIGAVEKMSKSKRNTVDPDDIIGSYGADTARWFMLSDSPPDRDVIWSEEGVQGAARFMQRLWRLVNEAADAGKAAPQDRPATFGSDALTLRKAAHGALDRVSTGIERLHFNVCLANIREFANELADALARSRSNKSAPARDLAPDLSWSLREAAIILVQIFSPMMPHLAEECWQRALGQTGLVSQARWPQIEPDLLIEDSITLPVQVNGKKRGEVTVASDAGNPEIEAAVLALDAVKQALGGKQARKIIIVPRRIVSVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWI5</accession>
    <name>Q3SWI5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">YkuD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0088</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0088"/>
    <dbReference type="EnsemblBacteria" id="ABA03356">
        <property type="protein sequence ID" value="ABA03356"/>
        <property type="gene ID" value="Nwi_0088"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0088"/>
    <dbReference type="eggNOG" id="COG3786">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105370_0_0_5"/>
    <dbReference type="OMA" id="PDDGWCD"/>
    <dbReference type="OrthoDB" id="1915613at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16913">
        <property type="entry name" value="YkuD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005490">
        <property type="entry name" value="LD_TPept_cat_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03734">
        <property type="entry name" value="YkuD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YkuD" evidence="1">
        <location>
            <begin position="40"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="189" mass="21139" checksum="ADB89CE5560A1DFD" modified="2005-10-11" version="1">MQSSYSSMPCTTNSCDRPLAAIRIRAAAGRPQRGWLIAEGRAIAVALGRGGIRANKREGDGGTPKGIFRPVRLWWRPDRHPRPRTFLPVRAITPMDGWCEDPADRRYNRPIRLTPHATGDRLSRDDHLYDFIIEIDHNTRPRVAGRGSAVFLHLARDNFGPTAGCVAMTRPAMLRLLRRIGPCTKIVIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWK4</accession>
    <name>Q3SWK4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Translation initiation factor IF-3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">infC</name>
        <name evidence="8" type="ordered locus">Nwi_0069</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 5">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the IF-3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0069"/>
    <dbReference type="EnsemblBacteria" id="ABA03337">
        <property type="protein sequence ID" value="ABA03337"/>
        <property type="gene ID" value="Nwi_0069"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0069"/>
    <dbReference type="eggNOG" id="COG0290">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054919_3_2_5"/>
    <dbReference type="OMA" id="RNMIMFL"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00080">
        <property type="entry name" value="IF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036788">
        <property type="entry name" value="T_IF-3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036787">
        <property type="entry name" value="T_IF-3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019813">
        <property type="entry name" value="Translation_initiation_fac3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001288">
        <property type="entry name" value="Translation_initiation_fac_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019815">
        <property type="entry name" value="Translation_initiation_fac_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019814">
        <property type="entry name" value="Translation_initiation_fac_3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10938">
        <property type="entry name" value="PTHR10938"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00707">
        <property type="entry name" value="IF3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05198">
        <property type="entry name" value="IF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54364">
        <property type="entry name" value="SSF54364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55200">
        <property type="entry name" value="SSF55200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00168">
        <property type="entry name" value="infC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00938">
        <property type="entry name" value="IF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4 5" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 4 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF3_N" evidence="7">
        <location>
            <begin position="10"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="IF3_C" evidence="6">
        <location>
            <begin position="86"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA03337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="171" mass="19469" checksum="87EC01ECBCFB4611" modified="2005-10-11" version="1">MPVAKDGPRTNDEIRNAQVQLIDQEGTNQGTVETIAAIKMAMEAGMDLVEIAPNSSPPVCKIMDYGKYKFQAQKKAAEARKRQKIVEIKEIKLRPMIDDHDYNVKMKAMQRFFEEGDKVKITLRYRGREMAHQEIGTKLLDKVKADVSAYAKVEQDARFEGRQVVMVLAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWM1</accession>
    <name>Q3SWM1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dihydrofolate synthase/folylpolyglutamate synthase</fullName>
            <ecNumber evidence="6">6.3.2.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Folylpoly-gamma-glutamate synthetase-dihydrofolate synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Folylpolyglutamate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">Nwi_0052</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Functions in two distinct reactions of the de novo folate biosynthetic pathway. Catalyzes the addition of a glutamate residue to dihydropteroate (7,8-dihydropteroate or H2Pte) to form dihydrofolate (7,8-dihydrofolate monoglutamate or H2Pte-Glu). Also catalyzes successive additions of L-glutamate to tetrahydrofolate or 10-formyltetrahydrofolate or 5,10-methylenetetrahydrofolate, leading to folylpolyglutamate derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(6R)-5,10-methylenetetrahydrofolyl-(gamma-L-Glu)(n) + ATP + L-glutamate = (6R)-5,10-methylenetetrahydrofolyl-(gamma-L-Glu)(n+1) + ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:51912"/>
            <dbReference type="Rhea" id="RHEA-COMP:13257"/>
            <dbReference type="Rhea" id="RHEA-COMP:13258"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:136572"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>10-formyltetrahydrofolyl-(gamma-L-Glu)(n) + ATP + L-glutamate = 10-formyltetrahydrofolyl-(gamma-L-Glu)(n+1) + ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:51904"/>
            <dbReference type="Rhea" id="RHEA-COMP:13088"/>
            <dbReference type="Rhea" id="RHEA-COMP:14300"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:134413"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>7,8-dihydropteroate + ATP + L-glutamate = 7,8-dihydrofolate + ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23584"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17839"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the folylpolyglutamate synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.12" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313391.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0052"/>
    <dbReference type="EnsemblBacteria" id="ABA03320">
        <property type="protein sequence ID" value="ABA03320"/>
        <property type="gene ID" value="Nwi_0052"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0052"/>
    <dbReference type="eggNOG" id="COG0285">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015869_1_1_5"/>
    <dbReference type="OMA" id="YFEMGTL"/>
    <dbReference type="OrthoDB" id="1232874at2"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00157"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008841">
        <property type="term" value="F:dihydrofolate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001645">
        <property type="entry name" value="Folylpolyglutamate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018109">
        <property type="entry name" value="Folylpolyglutamate_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136">
        <property type="entry name" value="PTHR11136"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001563">
        <property type="entry name" value="Folylpolyglu_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01499">
        <property type="entry name" value="folC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01012">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="12" id="KW-0133">Cell shape</keyword>
    <keyword evidence="15" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="11" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="18 20" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase_M" evidence="19">
        <location>
            <begin position="53"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00000809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00002714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00019357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00030048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00032510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR001563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ABA03320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="442" mass="47522" checksum="458E6247F1EAAD22" modified="2005-10-11" version="1">MSAVPPRQSPLDDAVARISKLHPKRIDLTLGRMWRVLERLDHPERRLPPVIHVAGTNGKGSTVAYLRAILEAAGLRVHVFTSPYLVRINECVRLAGTLVADDALRDALLECERANGGEPLTLFEIKTAAAFLLFARSPADVLLLEVGLGGRLDSTNVVETPLASVITPVGIDHVEFLGGSLAEIAGEKAGIIKPGVPVICAEQSPEAEVVIEQRAKRMRSPLHAAGQHWHVHAEHGRLVYQDESGLLDLAAPRLFGRHQFDNAGLAIATLRAQDRFSIETTAFERGVTGAEWPARMQRLTSGRIVDQAPRDAELWLDGGHNADGGRIAAAALGDLEERVSRPLVVITGMMGNKDAKGFLANFAGLTRHVIAVPIPNMENAMPPEVLAEAVRALDMRAETAVSVAAALRSVARLAYEIPPRILITGSLYLAGHVLSENGTPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWM5</accession>
    <name>Q3SWM5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nucleotidyl transferase</fullName>
            <ecNumber evidence="2">2.7.7.13</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0048</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.13" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313387.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0048"/>
    <dbReference type="EnsemblBacteria" id="ABA03316">
        <property type="protein sequence ID" value="ABA03316"/>
        <property type="gene ID" value="Nwi_0048"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0048"/>
    <dbReference type="eggNOG" id="COG1208">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_2_1_5"/>
    <dbReference type="OMA" id="INHAHLG"/>
    <dbReference type="OrthoDB" id="1557977at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004475">
        <property type="term" value="F:mannose-1-phosphate guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transf_3" evidence="1">
        <location>
            <begin position="8"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="241" mass="25806" checksum="B973C11C1667FDB1" modified="2005-10-11" version="1">MPTTPTKAMVLAAGLGLRMRPLTERMPKPMVPVAGKPLLDHVLDRLADAGVSEAVVNVRYLPDQIIDHVAHRDRPKIIISDERDQVLGTGGAVVKALPLLGDAPFFHLNADTIWIDGARPNLTRLTEAFDPASMDILLLMAPTASSIGYSGSGDYAMLPNGALRRRQENQIVPFVYAGAAILSPALFDGAPAGEFPLTAIFDRAGERDRLFGLRLDGVWMHVGTPDAVQAAEKAVLASLDA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWN9</accession>
    <name>FABA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase</fullName>
            <ecNumber evidence="1">4.2.1.59</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabA</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Beta-hydroxydecanoyl thioester dehydrase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Trans-2-decenoyl-[acyl-carrier-protein] isomerase</fullName>
            <ecNumber evidence="1">5.3.3.14</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fabA</name>
        <name type="ordered locus">Nwi_0034</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Necessary for the introduction of cis unsaturation into fatty acids. Catalyzes the dehydration of (3R)-3-hydroxydecanoyl-ACP to E-(2)-decenoyl-ACP and then its isomerization to Z-(3)-decenoyl-ACP. Can catalyze the dehydratase reaction for beta-hydroxyacyl-ACPs with saturated chain lengths up to 16:0, being most active on intermediate chain length.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:13097"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="4.2.1.59"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(3R)-hydroxydecanoyl-[ACP] = (2E)-decenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41860"/>
            <dbReference type="Rhea" id="RHEA-COMP:9638"/>
            <dbReference type="Rhea" id="RHEA-COMP:9639"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78466"/>
            <dbReference type="ChEBI" id="CHEBI:78467"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2E)-decenoyl-[ACP] = (3Z)-decenoyl-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:23568"/>
            <dbReference type="Rhea" id="RHEA-COMP:9639"/>
            <dbReference type="Rhea" id="RHEA-COMP:9927"/>
            <dbReference type="ChEBI" id="CHEBI:78467"/>
            <dbReference type="ChEBI" id="CHEBI:78798"/>
            <dbReference type="EC" id="5.3.3.14"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioester dehydratase family. FabA subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.59" evidence="1"/>
    <dbReference type="EC" id="5.3.3.14" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313373.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWN9"/>
    <dbReference type="SMR" id="Q3SWN9"/>
    <dbReference type="STRING" id="323098.Nwi_0034"/>
    <dbReference type="EnsemblBacteria" id="ABA03302">
        <property type="protein sequence ID" value="ABA03302"/>
        <property type="gene ID" value="Nwi_0034"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0034"/>
    <dbReference type="eggNOG" id="COG0764">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097925_0_0_5"/>
    <dbReference type="OMA" id="FDCHFKG"/>
    <dbReference type="OrthoDB" id="1379832at2"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008659">
        <property type="term" value="F:(3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008693">
        <property type="term" value="F:3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047451">
        <property type="term" value="F:3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004317">
        <property type="term" value="F:3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034017">
        <property type="term" value="F:trans-2-decenoyl-acyl-carrier-protein isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01287">
        <property type="entry name" value="FabA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00405">
        <property type="entry name" value="FabA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010083">
        <property type="entry name" value="FabA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013114">
        <property type="entry name" value="FabA_FabZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30272">
        <property type="entry name" value="PTHR30272"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30272:SF8">
        <property type="entry name" value="PTHR30272:SF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07977">
        <property type="entry name" value="FabA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01749">
        <property type="entry name" value="fabA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword id="KW-0276">Fatty acid metabolism</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000267737" description="3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase">
        <location>
            <begin position="1"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00405"/>
        </source>
    </evidence>
    <sequence length="174" mass="19095" checksum="992057DFFEB5A92D" modified="2005-10-11" version="1">MQDRRSSYDYEGLLACGRGELFGPGNAQLPLPPMLMFDRITDINEDGGEFGKGLIRAELDVKPDLWFFGCHFKGDPVMPGCLGLDALWQMVGFFLGWTGGEGRGRALGLGELKFTGQVLPPVRKVVYNVDIKRVMRSKLVLGIADGWLSADDDIIYRAKDLKVGLFKQDAGPGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMV8</accession>
    <name>Q3SMV8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phage integrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_3136</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316291.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3136"/>
    <dbReference type="EnsemblBacteria" id="ABA06383">
        <property type="protein sequence ID" value="ABA06383"/>
        <property type="gene ID" value="Nwi_3136"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3136"/>
    <dbReference type="eggNOG" id="COG4974">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028383_0_0_5"/>
    <dbReference type="OMA" id="TTINFYC"/>
    <dbReference type="OrthoDB" id="862058at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyr recombinase" evidence="2">
        <location>
            <begin position="339"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="560" mass="61987" checksum="51469DA43EC718CF" modified="2005-10-11" version="1">MAKPSLLEPSFADALSAIEQSTDLSPQKRSQWASALRQIGKALDKPMETLPARWTSARFNVGRLHHAAVGANAKTLANQKSNVKAALCWFCNERDVAPRGVPFVAAWATLRESIDDSGCKRRLSGLMRYCSGCGIEPAAVDDATLDAYFVYRSQTTSLSTNLAARRSIARAWNVCVGHSAVWPQRKLAEPALQSADGPGWNDFPPNLQKDIDAYLASLQKIRKGTNGKRYRPCSASTIKTRRAELWAVARKAVRIGTPIGRLNSLAALVHPDVAGPVLDAYWRDNGDEPKIFTIELASKLLGLARYLRLDQEAIDRLEDYRAALEEHRQGGLTEKNLAVVRKVLTPGVWRSVVNLPGQLMRDARANLSYAPIKAALTAQIAVAISILCFAPIRLGNLGTIRMDQNLIKPGGPKSPFWLSFPHYDVKNRVSLDFTFDDELTALIDEYIHQYRPHLIRGANSDFLFPGVSGGPKTASMFSGQITDRIEEVAGLRLTVHQFRHAAAAIYLRHNPGAYEVVKRLLGHRNMQTTINFYCGLETVQATQQFGKMIRGQMTFEEEVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN41</accession>
    <name>Q3SN41_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">TonB-dependent receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_3051</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Cell outer membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TonB-dependent receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3051"/>
    <dbReference type="EnsemblBacteria" id="ABA06300">
        <property type="protein sequence ID" value="ABA06300"/>
        <property type="gene ID" value="Nwi_3051"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3051"/>
    <dbReference type="eggNOG" id="COG4206">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008287_10_1_5"/>
    <dbReference type="OMA" id="QWGADHA"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039426">
        <property type="entry name" value="BtuB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012910">
        <property type="entry name" value="Plug_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037066">
        <property type="entry name" value="Plug_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000531">
        <property type="entry name" value="TonB-dep_rcpt_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036942">
        <property type="entry name" value="TonB_rcpt_b-brl_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30069">
        <property type="entry name" value="PTHR30069"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07715">
        <property type="entry name" value="Plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00593">
        <property type="entry name" value="TonB_dep_Rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0798">TonB box</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229038" evidence="7">
        <location>
            <begin position="22"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="domain" description="Plug" evidence="9">
        <location>
            <begin position="61"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="TonB_dep_Rec" evidence="8">
        <location>
            <begin position="272"/>
            <end position="700"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA06300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="702" mass="75988" checksum="CEF5415F2486581E" modified="2005-10-11" version="1">MRFSRWFLQTCVMAFSSAAAAQTIDLPEVVVTSPNPIQTAPNSDEAGYPGATSQSIAAASSFTSVSVMTSQQGLTRGAASLGDALSTTPGVSSTTFSPVASRPVIRGLGGFRVRTQENGIGSHDMGNLGEDHAVTIDPLVAGRVEVIRGPGTLRYGSQAIGGVVSANNGRIPTVIPPNGVAFEARGGLNSVSGGKDGALLLDAGGGDFALHVDTFKRAAGDYRIPGGIQENSSYSSQGYSIGGSHLFKDGFVGVSYQSMATTYFIPGIDSAREKNHIDLRQSKWSSRGEWRLRESGIDTLRYWLGFTDYKHDEIDGLGAATNIGSTFRNRELESRVEVSHLPFSTAFGELTGAAGVQWGRRQLSVKGAQEELLSPARAENLAAFIFEELQLMPALRMQGAIRMERTAIAGTGASFPSSYLPPPDQPSLFAVNRSFLPVSASAGLLYDLPYGMVARVTAQHVERAPDPVELFYKGPHDTPRTFEIGDPTMTLEKADTLEIGLKRGRGDTRFEISAYYTKYQNFIYKNFTGLMCNDSFATCGAPGASFDQIIYSQRDATFTGGELQIEHDVARLWDGIWGVEAQYDVVRATFADGSYVPKIPPYRLGGGLFYRDPSWSARINLLHAFAQNNLGAFETPTPGYNLLNAEVSYMAKLPTPTFPTELTIGLRGENLLNDSIRFHQSYKKDEVLQPGRNIRLFASVKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN61</accession>
    <name>Q3SN61_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Secretion protein HlyD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_3030</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3030"/>
    <dbReference type="EnsemblBacteria" id="ABA06280">
        <property type="protein sequence ID" value="ABA06280"/>
        <property type="gene ID" value="Nwi_3030"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3030"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018816_7_0_5"/>
    <dbReference type="OMA" id="YVAERFI"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032317">
        <property type="entry name" value="HlyD_D23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006143">
        <property type="entry name" value="RND_pump_MFP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16576">
        <property type="entry name" value="HlyD_D23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01730">
        <property type="entry name" value="RND_mfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004228985" evidence="2">
        <location>
            <begin position="38"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="HlyD_D23" evidence="3">
        <location>
            <begin position="116"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA06280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="306" mass="31997" checksum="DE42DC5A59B56E3E" modified="2005-10-11" version="1">MRRLSDTFGPRCARKLVSGLLLGVAVAALGVMGPSLAADDTEPSAGALVTVARATNACFSEMVRVTGFIVARQEAVAGPDSEGSLVTDVLVREGDTVALNQELARLTPPPGGKSGSTLRAPAAGLVTQVRTAVGAPASPQAGPMFRIAVNNEIELDAEVPSIHVLKLNPGASARVSREDGPDLVGRVRLVSPEIDRKTQMGHVRLSLSADPSLRIGMFARASIDAKRSCGVAIPRSAIDHLTVQVVKGDIVEKRRVRIGLTSDTAVEILEGLKAGETVVADAGTSLHDGDRVKAIFTDDLDRSRVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN67</accession>
    <name>Q3SN67_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Trehalose 6-phosphate synthase</fullName>
            <ecNumber evidence="2">2.4.1.15</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_3024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 20 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.15" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316192.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3024"/>
    <dbReference type="CAZy" id="GT20">
        <property type="family name" value="Glycosyltransferase Family 20"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06274">
        <property type="protein sequence ID" value="ABA06274"/>
        <property type="gene ID" value="Nwi_3024"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3024"/>
    <dbReference type="eggNOG" id="COG0380">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002351_7_1_5"/>
    <dbReference type="OMA" id="YYGFSNR"/>
    <dbReference type="OrthoDB" id="556566at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003825">
        <property type="term" value="F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="PTHR10788"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="493" mass="53989" checksum="DEB570909D1FBA42" modified="2005-10-11" version="1">MSLVVVSNRVARARANEPLTGGLAAALLPVIEKSGAIWVGSSGRVRDGSHKEPFAEVEALGAGALALLDLPAAHYGGYYEGFANSALWPALHSRSDLIRVSHHDYQSYREVNAFMARALMRFRKPDTAFWIQDYHFLSLGAELRDLGVRNPIGFFLHTPWPARAIMGAVPHHRELIEAMLAYDLIGFQTDDDCENFLSYVQSELGLKIQDSVVTSRYGKSRCAVFPIGIDVEKFALQAAKASSHPDVSRLRRSLNGEQLAIGVDRLDYSKGLVNRINAFDRMLVLNPALKRTVSLLQIATPSRGTIEAYSNLKSELARLVTEVNGRHGETDWMPIRYLNRGYSQTVLAGLYRTARVGVVTPLHDGMNLVAKEYVAAQNPADPGVLVLSKFAGAANELQTALLVNPHEIDGMARAIAAAFAMPLTERRTRWEAMMRKLRAGTAQQWFTDFSEALEDARASNEKTASNCPAAAAAVTSKSWPLHLVGSRSGAGVH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN93</accession>
    <name>TRHO_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA uridine(34) hydroxylase</fullName>
            <ecNumber evidence="1">1.14.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA hydroxylation protein O</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trhO</name>
        <name type="ordered locus">Nwi_2998</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes oxygen-dependent 5-hydroxyuridine (ho5U) modification at position 34 in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AH2 + O2 + uridine(34) in tRNA = 5-hydroxyuridine(34) in tRNA + A + H2O</text>
            <dbReference type="Rhea" id="RHEA:64224"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="Rhea" id="RHEA-COMP:13381"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:136877"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TrhO family.</text>
    </comment>
    <dbReference type="EC" id="1.14.-.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316170.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SN93"/>
    <dbReference type="SMR" id="Q3SN93"/>
    <dbReference type="STRING" id="323098.Nwi_2998"/>
    <dbReference type="EnsemblBacteria" id="ABA06248">
        <property type="protein sequence ID" value="ABA06248"/>
        <property type="gene ID" value="Nwi_2998"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2998"/>
    <dbReference type="eggNOG" id="COG1054">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038878_0_1_5"/>
    <dbReference type="OMA" id="CDTHTNC"/>
    <dbReference type="OrthoDB" id="684577at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00469">
        <property type="entry name" value="TrhO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020936">
        <property type="entry name" value="TrhO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040503">
        <property type="entry name" value="TRHO_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43268">
        <property type="entry name" value="PTHR43268"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17773">
        <property type="entry name" value="UPF0176_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="SSF52821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000242926" description="tRNA uridine(34) hydroxylase">
        <location>
            <begin position="1"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="125"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine persulfide intermediate" evidence="1">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00469"/>
        </source>
    </evidence>
    <sequence length="255" mass="28304" checksum="C102DBE8A0BFBBE0" modified="2005-10-11" version="1">MLKVAALYQFTPLPDFRALREPLRELCAAHAIKGSILLAAEGINGTVAGTPRAIDALVSELQTGALFSGRLNDLELKFSQASAMPFARLKVRLKKEIVTLGDPATDPTRAVGIYVDPQDWNELIATPDTILLDVRNDFEVVMGSFEGAIDPQLARFGQFKDFAAQELDHRKHRRIAMYCTGGIRCEKASSYLLSRGFKEVYHLKGGILKYLEQIPESESRWRGECFVFDDRIALGHGLKESRQASPVMDGAPHDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNB6</accession>
    <name>Q3SNB6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ferrous iron transport protein B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2975</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316147.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2975"/>
    <dbReference type="EnsemblBacteria" id="ABA06225">
        <property type="protein sequence ID" value="ABA06225"/>
        <property type="gene ID" value="Nwi_2975"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2975"/>
    <dbReference type="eggNOG" id="COG0370">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013350_3_0_5"/>
    <dbReference type="OMA" id="PLGYDWK"/>
    <dbReference type="OrthoDB" id="304844at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015093">
        <property type="term" value="F:ferrous iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01879">
        <property type="entry name" value="FeoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011640">
        <property type="entry name" value="Fe2_transport_prot_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030389">
        <property type="entry name" value="G_FEOB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011642">
        <property type="entry name" value="Gate_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07664">
        <property type="entry name" value="FeoB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02421">
        <property type="entry name" value="FeoB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07670">
        <property type="entry name" value="Gate"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51711">
        <property type="entry name" value="G_FEOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="286"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="330"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="364"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="401"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="463"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="563"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="597"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="domain" description="FeoB-type G" evidence="2">
        <location>
            <begin position="8"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="626" mass="68132" checksum="97E96EAE765AAF91" modified="2005-10-11" version="1">MTAIEVNDWRIALVGTPNCGKTALFNALTGSRQKVANYAGVTVERKSGVLRTASGHRVDLIDLPGTYSLRGRSPDEDITRDVILGKLASESPPDFLLCVADATNLRVALRLVIELKRVHRPMLLVLNMIDIARRRGVDIDLDKLSAELGVPVVTSTAVRRGGIDDLLCRIDEIAGKPTTEIIASDWTAPAASDLRAAQREADRVIRAAVSEPARPDTWTTRVDAVLLHPVWGMVALLLLLFVMFQAVFAWAEPAMELISQGFDALGALAATVLPEGLLQSFIQNGVISGVGSVIVFLPQILILFLFILLLEDLGYMARAAFLMDRIMGGAGLHGRAFIPLLSSFACAIPGIMATRVIDNKRDRLTTIMVAPLMTCSARIPVYTLIIAAFVPNQPVLGVLGLQGLVMFGLYALGIFSALAVSFVANRLFWRDSATPPFMLELPDYKLPQLRSVLMNLYQRAMAFLKRAGTTIFSMMVLIWFLASFPRPPEGATEPAIDFSLAAMIGRWIEPVLAPIGFNWQISVALIPGMAAREVAVASLGTVYAIEGGNEAAEQVGQMLAGQWSFATALAFLAWYVFAPQCASTLAVIKRETGGWRWVLITFFYMLSMAYLAAFVTYRIALALGWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNJ9</accession>
    <name>Q3SNJ9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Amino acid ABC transporter substrate-binding protein, PAAT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2892</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the bacterial solute-binding protein 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316072.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2892"/>
    <dbReference type="EnsemblBacteria" id="ABA06142">
        <property type="protein sequence ID" value="ABA06142"/>
        <property type="gene ID" value="Nwi_2892"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2892"/>
    <dbReference type="eggNOG" id="COG0834">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019602_3_2_5"/>
    <dbReference type="OMA" id="TWSMARE"/>
    <dbReference type="OrthoDB" id="685252at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018313">
        <property type="entry name" value="SBP_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00497">
        <property type="entry name" value="SBP_bac_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01039">
        <property type="entry name" value="SBP_BACTERIAL_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="PBPb" evidence="4">
        <location>
            <begin position="41"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA06142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="345" mass="38864" checksum="7702F65BBD535B1C" modified="2005-10-11" version="1">MRTFRGGLLIGLAIATAIAAMAITYERYDTKTLKRTLRRDAVLCGVNTGLPGFSSSDEKGNWSGFDVDFCRAVAAAIFDDPSKVKFVPLAADERFKELQSRKVDILARNSTWSMSRETNFNLYFPAVAYYDGEGFMVRTSRKADSALDLGDTKICVQSATTTQLNLADYFRANNLKYEEIRFEKLEDVLRAYDEARCDVVTADVSQLYALRLRLTKPNESFILPDVISKEPLAPVVRQRDDDWMMIIKWTLYAMINAEELGVTSKNIDEAMKSTKPDVMRLVGTEGQYGEALGLTKDWAARIIRHVGNYGEVYDRNIGTESRLKIPRGLNQPWNAGGIMYAPPLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNK5</accession>
    <name>Q3SNK5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glyoxalase/bleomycin resistance protein/dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2886</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316066.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2886"/>
    <dbReference type="EnsemblBacteria" id="ABA06136">
        <property type="protein sequence ID" value="ABA06136"/>
        <property type="gene ID" value="Nwi_2886"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2886"/>
    <dbReference type="eggNOG" id="COG2764">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046006_17_1_5"/>
    <dbReference type="OMA" id="LEKQMWG"/>
    <dbReference type="OrthoDB" id="2051456at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06588">
        <property type="entry name" value="PhnB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028973">
        <property type="entry name" value="PhnB-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06983">
        <property type="entry name" value="3-dmu-9_3-mt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-dmu-9_3-mt" evidence="1">
        <location>
            <begin position="2"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="148" mass="16572" checksum="CB02F696C63425AD" modified="2005-10-11" version="1">MKIVTSLSFRGQCREAFEFYAKILGGTIKAAIPYADAPPDMPITDEKYKSWLMHCWLDAGDQSLMGADMDVGWASNIDKPKNGFDVTLHTQDMTEARRWFDQLSEGGKAGMPFSETFWSPGFGTLIDKFGIPWMINVIPSADWKPPQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNN3</accession>
    <name>Q3SNN3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2858</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316046.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2858"/>
    <dbReference type="EnsemblBacteria" id="ABA06108">
        <property type="protein sequence ID" value="ABA06108"/>
        <property type="gene ID" value="Nwi_2858"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2858"/>
    <dbReference type="eggNOG" id="COG3505">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2168310_0_0_5"/>
    <dbReference type="OMA" id="AMICQTA"/>
    <dbReference type="OrthoDB" id="246216at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="110" mass="12413" checksum="6882E26BF6D89C6F" modified="2005-10-11" version="1">MTPTKVLIGQMLVVFGITLAGTWAATQWVAYSLGFQQRLGEPWFLIANLPVYYPWRLFEWWYVYDAYASLPRGYRLRNGRAAHAQGGASLSRSPPVQSTSLVAQPGHARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNP0</accession>
    <name>Q3SNP0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2851</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316039.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2851"/>
    <dbReference type="EnsemblBacteria" id="ABA06101">
        <property type="protein sequence ID" value="ABA06101"/>
        <property type="gene ID" value="Nwi_2851"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2851"/>
    <dbReference type="eggNOG" id="COG3843">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027492_1_0_5"/>
    <dbReference type="OMA" id="IDHWNTE"/>
    <dbReference type="OrthoDB" id="929753at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021795">
        <property type="entry name" value="DUF3363"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11843">
        <property type="entry name" value="DUF3363"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="654" mass="72649" checksum="E67DF065BC39C95C" modified="2005-10-11" version="1">MAARDDDRFRPKVAPPKARGSARAPKFTSRVLKAASKIGPTSTGTLRRQPSRGGARFGRGHVAAKFAGQSLRGNARRVVIKTRLVNLAKVGSHATIRHLRYIERDGVTKDGSKARTYGPGTDEADIAAFEERGREDRHQFRFIVSAEDAGDIGDLKTYTRDLMSRMEADLRTKLDWVAVDHWNTDNPHTHIVLRGRHPDGSDLVIARDYISNGMRNRARELATEWLGPRTEMEIRDGILREADQERWTGLDRMIQREAEDGVVNLLSEPAVAQARFRHTSMIGRLQKLTEMGLADETGHCVWTLHADAEATLRRMSKRGDIIRTMQRAMTGLKRDFVVLDGATAHTPVVGRIVDKGLADELYDRGYLIVDGVDGRAHYVPLAANADLESLPIGGIVETRAATERTADRTIAGLAANGIYRTDHHLAIERAKATPEHDPDSFVAAHVRRLEALRRVGIVERVEDGVWRVPADLAERGKAYDAKRLGGVDVELCSHLPIDRQTRAIGATWLDRQRFGDLSGLGQSGFGAEVRDAMRNREDYLVDQGFAERRGQRVIFARNLLATLRNREIEAAAKEIAGETGLPHHVVQDGERISGIYRRSVMLASGRFAMLDDGMGFSLVPWRPVLEKHLGQSVAGIARGNSVSWMFGRRRGMGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNP5</accession>
    <name>Q3SNP5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Peptidase C14, caspase catalytic subunit p20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2846</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2846"/>
    <dbReference type="MEROPS" id="C14.026"/>
    <dbReference type="EnsemblBacteria" id="ABA06096">
        <property type="protein sequence ID" value="ABA06096"/>
        <property type="gene ID" value="Nwi_2846"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2846"/>
    <dbReference type="eggNOG" id="COG4249">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018712_0_0_5"/>
    <dbReference type="OMA" id="TYKTGFY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029030">
        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001309">
        <property type="entry name" value="Pept_C14_p20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="SSF52129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50208">
        <property type="entry name" value="CASPASE_P20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CASPASE_P20" evidence="2">
        <location>
            <begin position="11"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="408"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="590"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="647"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="597"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="653"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="708" mass="77350" checksum="E697A7B80A1AF45D" modified="2005-10-11" version="1">MALSSGAAWADKRIALVVGNSSYQTVPQLPNPARDAKAVGQMFEDAGFDSVEVLLDLGNLDFKRAIRKFETDADQADIAVIYYAGHGIEVNGNNFLIPVDARLSSDRDAEDEAIRLERMVLSADGAKKLRVIILDACRDNPFTVKMRRERRAALRAISSGLGKVEPTSTDTLIAYAAKAGSTADDGEGRHSPFTTALLKNLTVPGLDIRLAFGRVRDEVMKLTGARQEPFVYGSLGGGNISLVPPPVVEKEPEAADVKGDFELVAKINSPRAWEVFISTYKTGFYADLARAQLASLTSKTATDIVRDVGNVTVAALPQDTPAPVNEPSSRERLEFDRLKDSTDQAALKRFIKRYPDSPLALQVQHRLEVLQNAAAEREEKQRAEREAARLAAEQARIEAERRKAELAAARKREEDERRAKAEEAAQQARAAEAERKAAEAKRKAEEAQRKAEERAAAKTAAEAARQKAESERAARQAELERRKAEEERRKAELAKQKEEACKAEEARFKELIANGGEGKGLDNVKAFAREATCARLQPQVASTIKTFEDEIARRLVMTAQVELGRIGCFAGDADGQLGPATRTALNRYQAAKNPSGKAGSDKRRNNGDLPLTDDVVKELGQQSGRVCPLACKTGEALKDDTCVAVTKPQPPATVQHKRDEKATASKPARRPERTQTSRQREPAPRARQEASRPSSSYRAAPAMIGVGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNU4</accession>
    <name>Q3SNU4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2794</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315992.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06047">
        <property type="protein sequence ID" value="ABA06047"/>
        <property type="gene ID" value="Nwi_2794"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2794"/>
    <dbReference type="eggNOG" id="ENOG5030XZN">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2002126_0_0_5"/>
    <dbReference type="OMA" id="KRSWLDA"/>
    <dbReference type="OrthoDB" id="1731071at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="130" mass="14123" checksum="6D63A966424966F8" modified="2005-10-11" version="1">MTLKISTATGAPRIIALSLAAAFIAVGFSASDASAKQRRYLDRDSAYGPSGPNVSYQSGPRTRVYISKRSWLDAGTEVLPGERKYTDYAFPPSVGYPSFARENLNRPIDRQPLSSPADLGGRYPTGFPLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNW5</accession>
    <name>Q3SNW5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA polymerase sigma factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2773</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315971.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2773"/>
    <dbReference type="EnsemblBacteria" id="ABA06026">
        <property type="protein sequence ID" value="ABA06026"/>
        <property type="gene ID" value="Nwi_2773"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2773"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_1_4_5"/>
    <dbReference type="OMA" id="NWNSFRK"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000838">
        <property type="entry name" value="RNA_pol_sigma70_ECF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01063">
        <property type="entry name" value="SIGMA70_ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="3">
        <location>
            <begin position="15"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="4">
        <location>
            <begin position="103"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA06026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="180" mass="19962" checksum="36D8A7C9EF7662DE" modified="2005-10-11" version="1">MPLTDSLRDEILASVPNLRAFAISLSGNGDRADDLVQETLLRALANIDSFQPGSNLPAWLFTILRNLFRSDYRKRRREVEDSDGNYAKTLKSQPSQNAHLEFEEFRAALDKLPQDQREALILVGASGFSYEDAAKICGCAVGTIKSRVNRARSKLSALLYVEGTEDFGPDDTVRAVIGNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNW9</accession>
    <name>Q3SNW9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2769</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315967.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2769"/>
    <dbReference type="EnsemblBacteria" id="ABA06022">
        <property type="protein sequence ID" value="ABA06022"/>
        <property type="gene ID" value="Nwi_2769"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2769"/>
    <dbReference type="eggNOG" id="ENOG5030X50">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150006_0_0_5"/>
    <dbReference type="OMA" id="MVACEPV"/>
    <dbReference type="OrthoDB" id="1919701at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="127" mass="13344" checksum="0F3EFB2952818BB4" modified="2005-10-11" version="1">MLQVSAGFLAGLAVTLSLGAVHLESNGEAAVYDAAVASVHRAVKSDLGIVPSPNAGGRTVSVRLQSLPRMSIVFRLPDSGQEARDRGVKRGLQRSPAARVKRTIACEPVVSMLTDVAKRLQPGRCVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNY9</accession>
    <name>Q3SNY9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2749</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315947.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2749"/>
    <dbReference type="EnsemblBacteria" id="ABA06002">
        <property type="protein sequence ID" value="ABA06002"/>
        <property type="gene ID" value="Nwi_2749"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2749"/>
    <dbReference type="eggNOG" id="COG5509">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_189918_3_0_5"/>
    <dbReference type="OMA" id="ARPHEIG"/>
    <dbReference type="OrthoDB" id="2069680at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009579">
        <property type="entry name" value="DUF1192"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06698">
        <property type="entry name" value="DUF1192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="62" mass="7068" checksum="01BC5B197DB009F2" modified="2005-10-11" version="1">MTIDGDEPRKKIAHEIGQDLSLLSVNELNERIALLTEEIQRLRTAEAKKRASKDAADRFFKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNZ0</accession>
    <name>Q3SNZ0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2748</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315946.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2748"/>
    <dbReference type="EnsemblBacteria" id="ABA06001">
        <property type="protein sequence ID" value="ABA06001"/>
        <property type="gene ID" value="Nwi_2748"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2748"/>
    <dbReference type="eggNOG" id="COG5317">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114005_0_0_5"/>
    <dbReference type="OMA" id="EAMYLST"/>
    <dbReference type="OrthoDB" id="1720716at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038301">
        <property type="entry name" value="AraC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010848">
        <property type="entry name" value="DUF1465"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07323">
        <property type="entry name" value="DUF1465"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="182" mass="20367" checksum="A0BE01EBAE0BAE08" modified="2005-10-11" version="1">MFDPSAGGTGLVQFSERLAGSSVFTALFREGMDLVEETAAYLDGDGRSEAKTLERAVSLTYATESMRLTTRLMQLASWLLLHRAVKEGEMTLAQANREKTRVKLTAADPGPHDMIAKLPWQLQDLIERSMNLQARIRRLDTTIHAPPLERGTADNPLVSQLNLLREAFERHRPADRVRGPRS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-16" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP14</accession>
    <name>RUVB_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Holliday junction ATP-dependent DNA helicase RuvB</fullName>
            <ecNumber evidence="1">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ruvB</name>
        <name type="ordered locus">Nwi_2724</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a complex with RuvA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RuvB family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315923.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SP14"/>
    <dbReference type="SMR" id="Q3SP14"/>
    <dbReference type="STRING" id="323098.Nwi_2724"/>
    <dbReference type="EnsemblBacteria" id="ABA05977">
        <property type="protein sequence ID" value="ABA05977"/>
        <property type="gene ID" value="Nwi_2724"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2724"/>
    <dbReference type="eggNOG" id="COG2255">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055599_1_0_5"/>
    <dbReference type="OMA" id="IHRMSRP"/>
    <dbReference type="OrthoDB" id="874197at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00016">
        <property type="entry name" value="DNA_helic_RuvB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041445">
        <property type="entry name" value="AAA_lid_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004605">
        <property type="entry name" value="DNA_helicase_Holl-junc_RuvB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008824">
        <property type="entry name" value="RuvB-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008823">
        <property type="entry name" value="RuvB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42848">
        <property type="entry name" value="PTHR42848"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17864">
        <property type="entry name" value="AAA_lid_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05491">
        <property type="entry name" value="RuvB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05496">
        <property type="entry name" value="RuvB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00635">
        <property type="entry name" value="ruvB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0742">SOS response</keyword>
    <feature type="chain" id="PRO_0000235382" description="Holliday junction ATP-dependent DNA helicase RuvB">
        <location>
            <begin position="1"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="329"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="61"/>
            <end position="68"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="348" mass="37800" checksum="B98B22D3DAFE9108" modified="2005-10-11" version="1">MTEASRIVAPERHADDVGDTALRPQKLSEFIGQRQARANLQIFIDAARKREEALDHVLFVGPPGLGKTTLAQIVARELGVGFRATSGPVIAKAGDLAALLTNLEERDVLFIDEIHRLSPAVEEVLYPAMEDFQLDLIIGEGPAARSVKIELAKFTLVGATTRAGLLTNPLRDRFGIPVRLNFYTEDELEKIVSRGARVLNVGMTPDGANEIARRARGTPRIAGRLLRRVRDFAEAADAAAIDRAVADHALGALEVDAAGLDAMDRRYLTTIALSYGGGPVGVETMAAALSEPRDAIEDIIEPYLIQCGYLQRTPRGRLLTSHAFRHLGLTEPSRDPAQSGLFGQDEDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP34</accession>
    <name>Q3SP34_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycine cleavage T protein (Aminomethyl transferase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2704</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315903.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2704"/>
    <dbReference type="EnsemblBacteria" id="ABA05957">
        <property type="protein sequence ID" value="ABA05957"/>
        <property type="gene ID" value="Nwi_2704"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2704"/>
    <dbReference type="eggNOG" id="COG0354">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007884_7_1_5"/>
    <dbReference type="OMA" id="MDRLHGV"/>
    <dbReference type="OrthoDB" id="1984573at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045179">
        <property type="entry name" value="YgfZ/GcvT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017703">
        <property type="entry name" value="YgfZ/GcvT_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22602">
        <property type="entry name" value="PTHR22602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03317">
        <property type="entry name" value="ygfZ_signature"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="293" mass="30808" checksum="63DA0BA7937417DA" modified="2005-10-11" version="1">MKAAFLPDRGVVKVSGDDARNFLNGLVTTDVTKVQPGLGRFGALLTPQGKITVDFLITEAQPGHGGGFLIDCPLPLAQPLATKLGFYKLRAKVTVENLSGKLGVLAAWDGEPAMHPDLTFADPRSDRLGWRSLVPEELAARAATVIGAELVESADYEAHRIRAGVPSGGADFNFGDAFPHEANMDRLHGVDFDKGCYVGQEVVSRMEHRGTARNRIVRVHLDGGAPEPGTAVVAGEKPVGTMGSSAADQGLALLRLDRAADAIEAGIPLTAGGVPIRVAEPDELKLPAKNTVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP59</accession>
    <name>Q3SP59_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DedA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2679</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2679"/>
    <dbReference type="EnsemblBacteria" id="ABA05932">
        <property type="protein sequence ID" value="ABA05932"/>
        <property type="gene ID" value="Nwi_2679"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2679"/>
    <dbReference type="eggNOG" id="COG1238">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098634_1_0_5"/>
    <dbReference type="OMA" id="RLYDWTM"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="SNARE_assoc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="SNARE_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="198" mass="21781" checksum="58E420E0CB133AE3" modified="2005-10-11" version="1">MVDRAMLRRTYDWCIAAADKPHALWILAAVAFAESSFFPVPPDVMLLPMALARPRRAWLFATVCTIASVAGGVLGYAIGALLYDSVGQWLIHLYGLGDKVEAFRASYAEWGAWIIIGKGLTPIPYKLVTITSGFAGYNVWLFILCSIIARGGRFFIVAVVLDRYGEAIRREIEKRLGLWAAIGAAVIVLGFVIAFRLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP92</accession>
    <name>Q3SP92_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycoside hydrolase 15-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2646</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2646"/>
    <dbReference type="CAZy" id="GH15">
        <property type="family name" value="Glycoside Hydrolase Family 15"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05899">
        <property type="protein sequence ID" value="ABA05899"/>
        <property type="gene ID" value="Nwi_2646"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2646"/>
    <dbReference type="eggNOG" id="COG3387">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010399_2_0_5"/>
    <dbReference type="OMA" id="HAEVCEY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011613">
        <property type="entry name" value="GH15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045582">
        <property type="entry name" value="Trehalase-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19291">
        <property type="entry name" value="DUF5911"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00723">
        <property type="entry name" value="Glyco_hydro_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5911" evidence="2">
        <location>
            <begin position="35"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_hydro_15" evidence="1">
        <location>
            <begin position="248"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="629" mass="70581" checksum="306B7F87A04B79A4" modified="2005-10-11" version="1">MANRSPMNNTDRFVILFRLASCFQLYPPRSAKLPSRIEDYGIIGDGETVALVDRTGSIDWLCWPCFDSDACFSALLGDERNGHWRIAPSEKIEHSSHGYRGDGLILETRFETAGGAVTLIDFMPPRDTATDIVRLVRGDRGRVRMHMDLVIRFGFGKDVPWVRRTEDRALLAICGPDMLVLRTPVETRGENMTTVADFEVSAGQTIPFVMAYSASHLAVPAPIDPAAALRDTEAFWRDWCGKFDYDGPYRDLAMRSLVALKAMIYAPSGGIVAAPTTSLPEKLGGARNWDYRFCWLRDATFTLLALMNSGYHDEASDWHDWLLRSVAGSPADMQIMYGILGQRRLTEWEADWLPGYEGARPVRIGNAAYAQLQLDVFGELMDAFHQSREANLKLREPTWDLECQLLEHLGRIWNETDSGIWELRGPGKHYVSSKVLAWVAFDRGVKSVEHFGFHGPVERWRELREMIHRDVCEKGFDREQNTFVESYGSKLLDASTLLIPSVGFLPASDPRVAGMLAAVEQHLMPDGFVLRHDPRAVTDEQPPIEGAFLACTLWLADAYVLRGDIERAEVLLKRVAGVANDLGLLSEEYDTVARRMTGNFPQALTHIALVNTIHNLNAARKPAMQRAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPB3</accession>
    <name>Q3SPB3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2625</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315828.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2625"/>
    <dbReference type="EnsemblBacteria" id="ABA05878">
        <property type="protein sequence ID" value="ABA05878"/>
        <property type="gene ID" value="Nwi_2625"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2625"/>
    <dbReference type="eggNOG" id="COG3816">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096796_1_0_5"/>
    <dbReference type="OMA" id="PDFCGDI"/>
    <dbReference type="OrthoDB" id="1518356at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010707">
        <property type="entry name" value="DUF1285"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023361">
        <property type="entry name" value="DUF1285_beta_roll_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06938">
        <property type="entry name" value="DUF1285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029557">
        <property type="entry name" value="UCP029557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="201" mass="22090" checksum="1CAF39EFF22EF9DB" modified="2005-10-11" version="1">MAKQGQPESRTLDGLISAARDAAGPPGKGLPPVHLWNPPFCGDLDMRIASDGTWFYMRTPIGRPALVRLFSTILKREGDSHFLVTPVEKVGISVDDAPFLAVEMIEDRDAGGRLLHFRTNVGDWVPCDSGHWLRFEPSAGGGLKPYLHVRAGLWAKVTRALYYDLVDMGAQRMVDNKMMFGIESGGEFFAMADAEQVRDAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPE9</accession>
    <name>Q3SPE9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Peptidase S49</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2589</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2589"/>
    <dbReference type="EnsemblBacteria" id="ABA05842">
        <property type="protein sequence ID" value="ABA05842"/>
        <property type="gene ID" value="Nwi_2589"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2589"/>
    <dbReference type="eggNOG" id="COG0616">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046540_1_0_5"/>
    <dbReference type="OMA" id="MIACAGD"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07023">
        <property type="entry name" value="S49_Sppa_N_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002142">
        <property type="entry name" value="Peptidase_S49"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01343">
        <property type="entry name" value="Peptidase_S49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_S49" evidence="1">
        <location>
            <begin position="89"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="292" mass="31004" checksum="4CB7831298266DBF" modified="2005-10-11" version="1">MSRVKTALTKLIPRRWRRGTSVVPVVRLSGVIGAVTPLRPGMTLAGVARTLERAFAVADAKAVALVVNSPGGSPVQSRQIYLRIRQLSREKEIPVLVFVEDVAASGGYMIACAGDEIFCDPSSILGSIGVVGGGFGFTELIRKIGVERRLYTAGEHKATLDPFLPENPDDVARLKAIQREIHATFIELVKASRGGRLKGADDLLFTGEYWAGERSVALGLADGIGDLRSTLRARFGDKVSTPLIAPSTGLLSGLLGRKAGAGTLTLQGGMAGMPDELISALETRAIWARFGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPJ3</accession>
    <name>Q3SPJ3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Peptidase M16</fullName>
            <ecNumber evidence="7">3.4.24.64</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2545</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the peptidase M16 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.64" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315749.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2545"/>
    <dbReference type="MEROPS" id="M16.019"/>
    <dbReference type="EnsemblBacteria" id="ABA05798">
        <property type="protein sequence ID" value="ABA05798"/>
        <property type="gene ID" value="Nwi_2545"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2545"/>
    <dbReference type="eggNOG" id="COG0612">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009902_1_0_5"/>
    <dbReference type="OMA" id="FFEHLLF"/>
    <dbReference type="OrthoDB" id="1188251at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="SSF63411"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229162" evidence="4">
        <location>
            <begin position="31"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M16" evidence="5">
        <location>
            <begin position="49"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M16_C" evidence="6">
        <location>
            <begin position="202"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="464" mass="50999" checksum="53E5F036F7BBF9CE" modified="2005-10-11" version="1">MMAARRVALSGFAALAVYLAALFTSPPAPAQTVTSPPPVTFTLANGLAVVVIPDHRTPVVTQMIWYKVGSADETPGKSGLAHFLEHLMFKGTARYPAGEFSQTVLRVGGEENAFTNFDYTGYYQRVPRDQLASMMAFEADRMTGLVLKDENVLPERDVVLEEYNMRVANNPDARLIEQIMAALYLNHPYGRPVIGWRQEIEKLTREDALAFYKRFYAPNNATLVIAGDVDAQKIRPEIEKTFGQVPSQPAIPSARIRPQEPLPAAPRTVTLADARVEQPMLRRYYLAPSATTAAAGESPALDVLAQLIGDGSNAYLYRALVVDKQLAVSTNATYQGTAVDASYFAIAVAPKPGANFTEIEQAIDAVIENLVKNPIPAEDLERVKTQLIAQAVYAQDSQTTLARWYGAGVTVGLSVDEIRNWPDRIRAVTAAQVQDVARKWLVKTRSVTGYLIKDTTPTREEKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPJ8</accession>
    <name>Q3SPJ8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Response regulator receiver</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2540</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315744.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2540"/>
    <dbReference type="EnsemblBacteria" id="ABA05793">
        <property type="protein sequence ID" value="ABA05793"/>
        <property type="gene ID" value="Nwi_2540"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2540"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_12_5"/>
    <dbReference type="OMA" id="AAGAHEY"/>
    <dbReference type="OrthoDB" id="1630092at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="8"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="131" mass="14545" checksum="81BEFFE29595BB83" modified="2005-10-11" version="1">MAVDFSMSILVVDDYNTMIRIIRNLLKQLGFENIDDASDGSAALNKMRTKKYGLVISDWNMEPMTGYDLLREVRADPNLATTPFIMVTAESKTENVIAAKKAGVNNYIVKPFNAATLKNKIEAVFPDNVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPQ4</accession>
    <name>Q3SPQ4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2484</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315690.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2484"/>
    <dbReference type="EnsemblBacteria" id="ABA05737">
        <property type="protein sequence ID" value="ABA05737"/>
        <property type="gene ID" value="Nwi_2484"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2484"/>
    <dbReference type="eggNOG" id="COG5304">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_179401_0_0_5"/>
    <dbReference type="OMA" id="RDVPYQS"/>
    <dbReference type="OrthoDB" id="1997676at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022148">
        <property type="entry name" value="CopG_antitoxin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12441">
        <property type="entry name" value="CopG_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="85" mass="9834" checksum="4D83E249B517275D" modified="2005-10-11" version="1">MSKPKPVPAFKSEAEERKFWETHDSTDYVDWSTAQRMRFPNLKPSTTAISLRLPVSLLEQIKIAANKRDVPYQSLIKMWLAEKVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="135" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPU7</accession>
    <name>Q3SPU7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA primase</fullName>
            <ecNumber evidence="12">2.7.7.101</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">dnaG</name>
        <name evidence="14" type="ordered locus">Nwi_2441</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Monomer. Interacts with DnaB.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the DnaG primase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.101" evidence="12"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315649.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2441"/>
    <dbReference type="EnsemblBacteria" id="ABA05694">
        <property type="protein sequence ID" value="ABA05694"/>
        <property type="gene ID" value="Nwi_2441"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2441"/>
    <dbReference type="eggNOG" id="COG0358">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013501_5_3_5"/>
    <dbReference type="OMA" id="DEPILCF"/>
    <dbReference type="OrthoDB" id="1071997at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003896">
        <property type="term" value="F:DNA primase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03364">
        <property type="entry name" value="TOPRIM_DnaG_primases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00974">
        <property type="entry name" value="DNA_primase_DnaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013264">
        <property type="entry name" value="DNA_primase_core_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037068">
        <property type="entry name" value="DNA_primase_core_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006295">
        <property type="entry name" value="DNA_primase_DnaG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036977">
        <property type="entry name" value="DNA_primase_Znf_CHC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030846">
        <property type="entry name" value="DnaG_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034151">
        <property type="entry name" value="TOPRIM_DnaG_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002694">
        <property type="entry name" value="Znf_CHC2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13662">
        <property type="entry name" value="Toprim_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08275">
        <property type="entry name" value="Toprim_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01807">
        <property type="entry name" value="zf-CHC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00400">
        <property type="entry name" value="ZnF_CHCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01391">
        <property type="entry name" value="dnaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 12" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 12 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2 12" id="KW-0639">Primosome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 12 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Toprim" evidence="13">
        <location>
            <begin position="257"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABA05694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="634" mass="71863" checksum="7E04C089B50B1393" modified="2005-10-11" version="1">MRFPPSFIDEVKARLPVSEVVGRRVKLKRAGREFKGLSPFQQEKTPSFTVNDQKQFYHDFSTGKHGNIFDFVMETEGVSFPEAVERLAAMAGLPLPRNSREDEQREQRRKSLFEVMELAAKYFEQNLASRVGAQARGYLSDRAIVPSSIVEFRIGYATADRFALKEYLGSKGVPVADMIEAGLLIAGDDIPVPYDRFRDRIMIPIHDQRGRLVGFGGRALRKDVQPKYLNSPETPIFHKGSVVFNFHRARQPAHEANSVVAVEGYMDAIAVYQAGIKAVVATMGTAFTEEQIATLWRLSPEPVVCFDSDRAGVAAAYRSIDRILPLLRVGKTFRFVFMDEQKDPDDLIREKGVEAFRNVLSGSLPLWDVLWQRETDGQDVRTPDKQAALEQRLYALVRTIQDPAVNTAFFRTSRMQLANLFWQVNRGPSRNQKEAGSKFVRNELRIRGEDQRSGLQKVLLGMLVQYPELLEDKRDVVLRLQFPDAFDQFLSHLFQLLVTEAKLSVSVIWERLDHEFYETLQTVHGDAGAGKPRGHRLFERFPVLKNDPPSDFVSGCLDHFIRILLWHQLEEDIERARLDAGEGDETAVARLLNLVREGHAEQEAISAEGVALSEWAEDIIRVWAPKGGTMHLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPV7</accession>
    <name>Q3SPV7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNA polymerase sigma factor RpoH</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">RNA polymerase sigma-32 factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpoH</name>
        <name evidence="9" type="ordered locus">Nwi_2430</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in regulation of expression of heat shock genes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with the RNA polymerase core enzyme.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the sigma-70 factor family. RpoH subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315642.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2430"/>
    <dbReference type="EnsemblBacteria" id="ABA05684">
        <property type="protein sequence ID" value="ABA05684"/>
        <property type="gene ID" value="Nwi_2430"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2430"/>
    <dbReference type="eggNOG" id="COG0568">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014793_3_5_5"/>
    <dbReference type="OMA" id="YQRWLAE"/>
    <dbReference type="OrthoDB" id="1565734at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00961">
        <property type="entry name" value="Sigma70_RpoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000943">
        <property type="entry name" value="RNA_pol_sigma70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009042">
        <property type="entry name" value="RNA_pol_sigma70_r1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012759">
        <property type="entry name" value="RNA_pol_sigma_RpoH_proteobac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00140">
        <property type="entry name" value="Sigma70_r1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000770">
        <property type="entry name" value="RNA_pol_sigma-SigE/K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00046">
        <property type="entry name" value="SIGMA70FCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02392">
        <property type="entry name" value="rpoH_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00715">
        <property type="entry name" value="SIGMA70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00716">
        <property type="entry name" value="SIGMA70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="2 6" id="KW-0346">Stress response</keyword>
    <keyword evidence="5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="1 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RNA_pol_sigma70" evidence="7">
        <location>
            <begin position="77"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_sigma70" evidence="8">
        <location>
            <begin position="253"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="6">
        <location>
            <begin position="254"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Sigma-70 factor domain-2" evidence="6">
        <location>
            <begin position="53"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Interaction with polymerase core subunit RpoC" evidence="6">
        <location>
            <begin position="77"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="299" mass="34222" checksum="106F42F97E2BA240" modified="2005-10-11" version="1">MARTATLPVLNGENGLSRYLSEIRKFPMLEPHEEYMFAKRLREHDDRDAAHHLVTSHLRLVAKIAMGYRGYGLPISEVVSEGNVGLMQAVKRFEPEKGFRLATYAMWWIKASIQEYILRSWSLVKMGTTANQKKLFFNLRKAKSKISALDEGDMRPDQVKLIAKRLGVTEQDVVDMNRRLGGDASLNAPIRDDGEAGEWQDWLVDQSPNQEAIMAEHEEFDHRRRALTGAMGVLNPRERRIFEARRLADEPMTLEDLAAEFNVSRERVRQIEVRAFEKVQSAVKTTIAQQEQAAMEAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPY9</accession>
    <name>Q3SPY9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UDP-N-acetylglucosamine 2-epimerase</fullName>
            <ecNumber evidence="2">5.1.3.14</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2398</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="5.1.3.14" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2398"/>
    <dbReference type="EnsemblBacteria" id="ABA05652">
        <property type="protein sequence ID" value="ABA05652"/>
        <property type="gene ID" value="Nwi_2398"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2398"/>
    <dbReference type="eggNOG" id="COG0381">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061127_0_0_5"/>
    <dbReference type="OMA" id="VTYHPVT"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008761">
        <property type="term" value="F:UDP-N-acetylglucosamine 2-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006047">
        <property type="term" value="P:UDP-N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020004">
        <property type="entry name" value="UDP-GlcNAc_Epase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003331">
        <property type="entry name" value="UDP_GlcNAc_Epimerase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029767">
        <property type="entry name" value="WecB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43174">
        <property type="entry name" value="PTHR43174"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02350">
        <property type="entry name" value="Epimerase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03568">
        <property type="entry name" value="NeuC_NnaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase_2" evidence="1">
        <location>
            <begin position="38"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="409" mass="43122" checksum="BB29E2547337F154" modified="2005-10-11" version="1">MAVNVTMTASRRRIAVVTGSRADYGLLRGLLTRLRGCAGIDLQVVACGMHLAPRFGETWRAIEADGFTLTGKVDLELGDDTPDAIARSTGIGTIGFSEAFQRLSPDLVVVLGDRFEILSAATAATLSNIPIAHIHGGEITAGAFDDAIRHAVTKMACIHFVAAEPYRLRVIQMGENPDFIFNVGAPGLDQADVAPALDRSSLFESLGIGEPRRFLLATLHPTTARPGADRATAAALLGALAQVKDRGIIFTGVNADPGNRIIDNAVRDFVSARPGHAYLFTSLGSDRYWSALRLTDVVIGNSSSGILEAPAVGTPSINIGDRQSGRLRAASILDCAVDQAEISAALSLVLEGRFQPDLSLEPPYGRGGASGRIADLIQSIDLTKAFPKTFHDLPPTTISQHSASAPAVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPZ4</accession>
    <name>Q3SPZ4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain dehydrogenase/reductase SDR</fullName>
            <ecNumber evidence="1">1.1.1.100</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2393</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.100" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315608.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2393"/>
    <dbReference type="EnsemblBacteria" id="ABA05647">
        <property type="protein sequence ID" value="ABA05647"/>
        <property type="gene ID" value="Nwi_2393"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2393"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_1_5"/>
    <dbReference type="OMA" id="VTEERCA"/>
    <dbReference type="OrthoDB" id="1294334at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="270" mass="28225" checksum="8CF7BD1BE4B1A523" modified="2005-10-11" version="1">MTPERTLRELMALDGRVALVTGGAGHIGRSISGALAELGARVAVVDLSSGAAGVVAADLKERWSVAAAGFECDLEDGAAVGDLPRRVHEALGRIDIIVNCAAFVGTSGLDGWAVGFEQQSAATWRRALEVNLTAPFILIQAAADDLRASGRGSVINVSSIYGLVGPDWRLYDDTTLGNPAAYAASKGGLVQLTRWLATTLAPDIRVNAIAPGGVFRSTPEPFLSRYVARTPLGRMAREEDLKGAVAYLASDLSAYVTGQCLAVDGGWTAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPZ5</accession>
    <name>Q3SPZ5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">LPS biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2392</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315607.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2392"/>
    <dbReference type="EnsemblBacteria" id="ABA05646">
        <property type="protein sequence ID" value="ABA05646"/>
        <property type="gene ID" value="Nwi_2392"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2392"/>
    <dbReference type="eggNOG" id="COG0037">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056004_1_0_5"/>
    <dbReference type="OMA" id="PFQPFIL"/>
    <dbReference type="OrthoDB" id="338203at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020022">
        <property type="entry name" value="N-acetyl_sugar_amidoTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03573">
        <property type="entry name" value="WbuX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="392" mass="46468" checksum="B6B22DFFFD4D5F0C" modified="2005-10-11" version="1">MLSCSKCLLPETAEATSFDDTGSCSVCRQIEVRDTQIDWDDRHRQMMELVAQYRDKGLYDCIVPYSGGKDSVFQLWYVVRKLGLKPLVVRFDHWFYRPLVEENNTSVFKQLGVDVLNFTPNWHVVRELMLESLKRRGDFCWHCHTGIYAHTMQIAIRYETPLLFWGESLAEYQSFYSYDEMEEVDEKRFNRAMNLGITADDMYEFLGGRVAKRDLYPFVYPPRKELMRIKCRSICLGSYIRWDTKKHVEIIKRELGWKGQEVEGIPPEYDYEKIECFFQGVRDYLKYIKRGMGRTNHLANIDIRNHRITREEGERLVKEYDGKRPPSLDLFLDYLQISEEQFLDVAMRHEVSPWQFSPNQVESGKRMPDMDKWDNTRVPWPNHPGIDIGNKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ06</accession>
    <name>Q3SQ06_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2381</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05635">
        <property type="protein sequence ID" value="ABA05635"/>
        <property type="gene ID" value="Nwi_2381"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2381"/>
    <dbReference type="eggNOG" id="ENOG5030256">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157974_2_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="63" mass="6623" checksum="22CF9D364A89F4CE" modified="2005-10-11" version="1">MSAGMGDMIPHWAGGLPADAPPRPGTAKYDEVMKQRERARLEPKPNEPGAAQSADGQEPAPAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="132" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ10</accession>
    <name>Q3SQ10_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Adenosylhomocysteinase</fullName>
            <ecNumber evidence="4">3.3.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">S-adenosyl-L-homocysteine hydrolase</fullName>
            <shortName evidence="4">AdoHcyase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ahcY</name>
        <name evidence="9" type="ordered locus">Nwi_2377</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May play a key role in the regulation of the intracellular concentration of adenosylhomocysteine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 6">
            <text>H2O + S-adenosyl-L-homocysteine = adenosine + L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:21708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="3.3.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4 6">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
        <text evidence="4 6">Binds 1 NAD(+) per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 6">Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 7">Belongs to the adenosylhomocysteinase family.</text>
    </comment>
    <dbReference type="EC" id="3.3.1.1" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315592.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2377"/>
    <dbReference type="EnsemblBacteria" id="ABA05631">
        <property type="protein sequence ID" value="ABA05631"/>
        <property type="gene ID" value="Nwi_2377"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2377"/>
    <dbReference type="eggNOG" id="COG0499">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025194_2_1_5"/>
    <dbReference type="OMA" id="NKYGCRE"/>
    <dbReference type="OrthoDB" id="522981at2"/>
    <dbReference type="UniPathway" id="UPA00314">
        <property type="reaction ID" value="UER00076"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004013">
        <property type="term" value="F:adenosylhomocysteinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00401">
        <property type="entry name" value="SAHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00563">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042172">
        <property type="entry name" value="Adenosylhomocyst_ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000043">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015878">
        <property type="entry name" value="Ado_hCys_hydrolase_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020082">
        <property type="entry name" value="S-Ado-L-homoCys_hydrolase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420">
        <property type="entry name" value="PTHR23420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05221">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00670">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001109">
        <property type="entry name" value="Ad_hcy_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00996">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00997">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00936">
        <property type="entry name" value="ahcY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00738">
        <property type="entry name" value="ADOHCYASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00739">
        <property type="entry name" value="ADOHCYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 4 6" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4 6" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AdoHcyase_NAD" evidence="8">
        <location>
            <begin position="244"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4 5">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 5">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 5">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="210"/>
            <end position="212"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 5">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 5">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="273"/>
            <end position="278"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="352"/>
            <end position="354"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="397"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001109-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="485" mass="53125" checksum="559C9FB63A2FA51C" modified="2005-10-11" version="1">MTTAAAATKQTADFTDYIVADIGLADFGRKEISLAETEMPGLMATREEYGPKQPLKGARIAGSLHMTIQTAVLIETLQALGADIRWVSCNIYSTQDHAAAAIAAAGIPVFAVKGETLKDYWDYTAKLFDWHGAKSGESLHPNMILDDGGDATLLIHLGVRAEKGDTAFLDKPGAEEEEVLFALIKRLLKEKPKGWFGEVAKSIQGVSEETTTGVHRLYEMQKNGTLLWPAINVNDSVTKSKFDNLYGCRESLVDGIRRGTDVMMSGKVAMVAGFGDVGKGSAASLRQAGCRVMVSEIDPICALQAAMEGYEVVTMEDAAPRADIFVTATGNKDIITIEHMRAMKDRAIVCNIGHFDNEIQVGALKNLKWDNIKPQVDEITFPDGKRMILLSEGRLVNLGNAMGHPSFVMSASFTNQTLAQIELFANNKDGRYKKEVYVLPKSLDEKVARLHLNKIGVTLTELRKDQADYIGVKVEGPFKSDHYRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ69</accession>
    <name>Q3SQ69_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="13">TonB-dependent siderophore receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Nwi_2318</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the TonB-dependent receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2318"/>
    <dbReference type="EnsemblBacteria" id="ABA05572">
        <property type="protein sequence ID" value="ABA05572"/>
        <property type="gene ID" value="Nwi_2318"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2318"/>
    <dbReference type="eggNOG" id="COG4773">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4774">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008287_9_2_5"/>
    <dbReference type="OMA" id="RQTFTFK"/>
    <dbReference type="OrthoDB" id="169533at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015344">
        <property type="term" value="F:siderophore uptake transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055072">
        <property type="term" value="P:iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012910">
        <property type="entry name" value="Plug_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037066">
        <property type="entry name" value="Plug_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039423">
        <property type="entry name" value="TonB-dep_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000531">
        <property type="entry name" value="TonB-dep_rcpt_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036942">
        <property type="entry name" value="TonB_rcpt_b-brl_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010917">
        <property type="entry name" value="TonB_rcpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010105">
        <property type="entry name" value="TonB_sidphr_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32552">
        <property type="entry name" value="PTHR32552"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07715">
        <property type="entry name" value="Plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00593">
        <property type="entry name" value="TonB_dep_Rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01783">
        <property type="entry name" value="TonB-siderophor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01156">
        <property type="entry name" value="TONB_DEPENDENT_REC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="2" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0410">Iron transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="5 9" id="KW-0798">TonB box</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Plug" evidence="12">
        <location>
            <begin position="118"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="TonB_dep_Rec" evidence="11">
        <location>
            <begin position="322"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="45"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="67"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA05572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="779" mass="83647" checksum="A22C129F9894B433" modified="2005-10-11" version="1">MSNDTALGRGGALCCVLATVAAIESCSTASAQSNSDTVLPAVTITEPARQPLRSQPNRAFRASAARRATHQSASRTPAVTPSAALPATAPGTMNAEGAPGPLNLGIPNAAGSLVGLTPRETPATVNIITQRDMQEKGLRTMVETFNSVPGVMSGNLPGEPGVISMRGFSRAATGYAVDGVRAPDPLIVSRDFDTFSFERVEILKGPASVIQGVGALAGAVNLVTKQPKLGQAGAQGLLSYGSFNSLRTGIDINAPIGSNAAVRSTLSYGQSDGFINDTRSRKVGATTSGTLAPTDRLTFTSSASFFHDEFRTPYQGTPLIARSLARDPTNLVSAPGGLVIDRSIRNQNYNVENGLMTSDTVWLRGGAEYKLTDNWTLRNDLSFFKSDRYWASSEDFTFNSVSGLLDRSTTKITHAHQIWSERAAAAYDGIIGGFRNRFAAGLEYTDTDFDSSRRFGSATPVDPFNPDRGYFPADTAANFPTRQDFTSGLKTFAAFAENAINLTPDWLMLASVRHESMKLDRRIDDLNTGTVTRFEKTFDNLSWRVGTVYNVTSGTALFGQYNRAAIPVSGLLLSNLANGRFDLSTGSSIEGGIKSSLLDNRLVTTASVYQIEQNNILTRDPTTPALTVQGGSQRSRGVELDVAVSLTPQWRVNANASFIKAEFTELRSATLDLRGNRPINVPAQAFMVSTSYRFDPVPLTIGASAQHVGSFYTDTANTIEVSGRTLLDAWVAYDIGGGTLRLRGRNLTNAFYANWSGYSPTQVYLGAPRNFDLSYNVRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQI2</accession>
    <name>Q3SQI2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aerobic-type carbon monoxide dehydrogenase small subunit CoxS/CutS</fullName>
            <ecNumber evidence="2">1.2.99.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2205</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.99.2" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315428.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2205"/>
    <dbReference type="EnsemblBacteria" id="ABA05459">
        <property type="protein sequence ID" value="ABA05459"/>
        <property type="gene ID" value="Nwi_2205"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2205"/>
    <dbReference type="eggNOG" id="COG2080">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052511_3_1_5"/>
    <dbReference type="OMA" id="QEADDVW"/>
    <dbReference type="OrthoDB" id="1843415at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002888">
        <property type="entry name" value="2Fe-2S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036884">
        <property type="entry name" value="2Fe-2S-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01799">
        <property type="entry name" value="Fer2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47741">
        <property type="entry name" value="SSF47741"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="1">
        <location>
            <begin position="2"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="161" mass="17344" checksum="9EE62072FDC11E03" modified="2005-10-11" version="1">MAKISLIVNGEPTTADIDPRTLLVRFLRENLRLTGTHVGCDTSQCGACIVHLDGKAVKSCTMLAVMADGHEIRTIEGLAADGAPLHPMQEAFREHHGLQCGFCTPGMIMSAIDMVRRKGYALDERTIREELEGNLCRCTGYQNIVASIAAGATAMAKSDRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQJ1</accession>
    <name>Q3SQJ1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2196</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315419.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2196"/>
    <dbReference type="EnsemblBacteria" id="ABA05450">
        <property type="protein sequence ID" value="ABA05450"/>
        <property type="gene ID" value="Nwi_2196"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2196"/>
    <dbReference type="eggNOG" id="COG1801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046519_1_1_5"/>
    <dbReference type="OMA" id="CYFDNDV"/>
    <dbReference type="OrthoDB" id="1068600at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002763">
        <property type="entry name" value="DUF72"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036520">
        <property type="entry name" value="UPF0759_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30348">
        <property type="entry name" value="PTHR30348"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01904">
        <property type="entry name" value="DUF72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117396">
        <property type="entry name" value="SSF117396"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="267" mass="30008" checksum="792EF2C32C16E2F4" modified="2005-10-11" version="1">MASKAGNIYIGIGGWTFAPWRGVFYPEKLAQAKELAYAASKLTSIEINGTYYGSQKPESFRKWALEVPDGFIFSLKGPRFATNRRVLAEAGDSVQRFYDSGVLALGDHLGPVLWQFAPTKAFDECDFGKFLEILPRKLEGHTLRHVVEVRHDSFRTPAFISLLRQFQVPVVFAEHGKYPAIADVVGDFVYARLQRGSDEIRTCYPPEQLDAWAKRFKSWAAGEEPDDLPRVDEARPKKAPRDVFAYVIHEGKVRAPAGAMELIARVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQM5</accession>
    <name>Q3SQM5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2162</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2162"/>
    <dbReference type="EnsemblBacteria" id="ABA05416">
        <property type="protein sequence ID" value="ABA05416"/>
        <property type="gene ID" value="Nwi_2162"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2162"/>
    <dbReference type="eggNOG" id="COG4976">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065329_0_0_5"/>
    <dbReference type="OMA" id="VCCSYGI"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="322" mass="35659" checksum="E8449750D687366E" modified="2005-10-11" version="1">MRLSKKDEALLKVDFSRINQAKAVFDDIYIQDDPRSYFSTLGDLDYMITDVAEPVIRQILSAKASVTESKPVVLDVGCSYGINAAVHRFPLTFGGLRHRYARREIKAVSSEELMQLDRNFYASWPDHGLARFIGLDVSAPAVRYATRVGLLDQGIVADLESQPLSAEDACIIKPADVILSTGCIGYVTEKTFGKLLDATEKKPWIISFVLRMFPYDSLEKTFAQRGLVTEKLGGVTFIQRRFRDAEEFENSLATLAAAHIDSTGLESEGLFHADLILSRPEADARVASLEDIVTVASGRGRPIAPRYVHVESDDGLQVTLEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQM6</accession>
    <name>Q3SQM6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nuclear protein SET</fullName>
            <ecNumber evidence="3">2.1.1.43</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2161</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.43" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315384.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2161"/>
    <dbReference type="EnsemblBacteria" id="ABA05415">
        <property type="protein sequence ID" value="ABA05415"/>
        <property type="gene ID" value="Nwi_2161"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2161"/>
    <dbReference type="eggNOG" id="COG2940">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020840_2_0_5"/>
    <dbReference type="OMA" id="NDHYRVG"/>
    <dbReference type="OrthoDB" id="1497183at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SSF82199"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SET" evidence="2">
        <location>
            <begin position="5"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="196" mass="22213" checksum="0BAD2FC933565535" modified="2005-10-11" version="1">MPVIPPNKPYRIGRSRTGLGLFATKPIKKGTKIIRYVGRLLDCTKEKDDAVENKYLFQITSRWTIDGSMRSNIARYINHSCKPNAESDVRKIKRRVDIRAIKDIAPGEEINYDYGTEYFKEYLKPIGCKCDSCEKKRKKLRAAAREEKKHSRGSAENNAGPKPGRRRLNGHKANSAAPKKSKTLSTAKGGRSKRFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQN9</accession>
    <name>Q3SQN9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Drug resistance transporter EmrB/QacA subfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_2148</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315371.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2148"/>
    <dbReference type="EnsemblBacteria" id="ABA05402">
        <property type="protein sequence ID" value="ABA05402"/>
        <property type="gene ID" value="Nwi_2148"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2148"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_0_5"/>
    <dbReference type="OMA" id="DFRWAFV"/>
    <dbReference type="OrthoDB" id="1558246at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004638">
        <property type="entry name" value="EmrB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00711">
        <property type="entry name" value="efflux_EmrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="260"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="293"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="428"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="7">
        <location>
            <begin position="7"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="466"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA05402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="490" mass="52023" checksum="7BDF865A9A47A6F8" modified="2005-10-11" version="1">MNKERLVPLIVAAALFMENMDSTVIATSLPAIAADIGASPLTLKLAITSYLLSLAVFIPASGWTADRFGARSVFSAAIVVFMIGSIGCALSSSVTDFVIARTLQGVGGAMMTPVGRLVLLRSIDKSALVNAMAWVTVPALIGPVVGPPLGGFITTYFSWHWIFLINIPVGLIGIFMAQRYIDPIRSDNPERFDLYGMVLAGIGVGGIAFGLSVAGLNLLPWTVVTALIGAGSVSMALYVMHARRTDQPVLDLTLLRLPTFRASIHGGFLFRVGVGAMPFLLPLLMQVGFGLSPFGSGLVTFSSAVGAIAMKTLAARIIRAFGFRNIMTVNAVVSSIFLAACALFTVTTPLLLIMIILVVGGFFRSLQFTAINALTYADVEPERMSRATTLASVNQQLAISAGVALGAFSVETTMLFRHGTELTAGDFGSGFLVVSLLSAMSTFYFMRLSADAGHQVSGRRMVIISDPTRESEAEEEASRETVTARDQRIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQQ9</accession>
    <name>Q3SQQ9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2127</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315353.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2127"/>
    <dbReference type="EnsemblBacteria" id="ABA05382">
        <property type="protein sequence ID" value="ABA05382"/>
        <property type="gene ID" value="Nwi_2127"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2127"/>
    <dbReference type="eggNOG" id="ENOG5032T4Y">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2539560_0_0_5"/>
    <dbReference type="OMA" id="MTPFGHL"/>
    <dbReference type="OrthoDB" id="1983513at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046120">
        <property type="entry name" value="DUF6117"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19612">
        <property type="entry name" value="DUF6117"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="80" mass="8472" checksum="53ADE1EDD24D8B74" modified="2005-10-11" version="1">MSIPDHARANFATLLRAAADGNLALMECSDAVTGEARYVICAVSRDGTDFMFTPFGHLADGNPFDAYVPPSATLPDEPTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQR9</accession>
    <name>Q3SQR9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Heat shock protein Hsp20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2117</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315345.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2117"/>
    <dbReference type="EnsemblBacteria" id="ABA05372">
        <property type="protein sequence ID" value="ABA05372"/>
        <property type="gene ID" value="Nwi_2117"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2117"/>
    <dbReference type="eggNOG" id="COG0071">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046737_4_2_5"/>
    <dbReference type="OMA" id="KESEYLH"/>
    <dbReference type="OrthoDB" id="1523369at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06470">
        <property type="entry name" value="ACD_IbpA-B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037913">
        <property type="entry name" value="ACD_IbpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="SSF49764"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="4">
        <location>
            <begin position="29"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="154" mass="17660" checksum="71F7AA3DC70B6D14" modified="2005-10-11" version="1">MRDFDLTPIWRSTVGFDRLVDLIDDSLRLAGEDNYPPYNIARTGEDNYRISLALAGFKPEEITVTAEQNMLTVEGRKAEKDDREYLYQGISGRPFRRQFNLADYVEVKGASFEDGLLQVDLVRELPEAMKPRRIAIKSGPFRSETQQIEQKQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR27</accession>
    <name>Q3SR27_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Abortive infection protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315250.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2005"/>
    <dbReference type="EnsemblBacteria" id="ABA05264">
        <property type="protein sequence ID" value="ABA05264"/>
        <property type="gene ID" value="Nwi_2005"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2005"/>
    <dbReference type="eggNOG" id="COG1266">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1033757_0_0_5"/>
    <dbReference type="OMA" id="WFALLNV"/>
    <dbReference type="OrthoDB" id="1623593at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="191"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="262" mass="28463" checksum="3E9974A1B743F25B" modified="2005-10-11" version="1">MTSIEAPVSSVATPPPLPPQPRLPQAWKFLGTSLWGLVVFAAMFAGQLSVLAYFVITDDAPFSLDEVAEQAESGLTVALSVVTGLPAVLLALWIAIRLSGVPFADYLALRRASWRHLLLGIVAMVVLVTGWEGISKAVGHESSPAFMVDVAKSARADGALWLLVVSFCVVAPVSEELLARGFLYRGWSESFLRPIGAIVLSSLVWTAMHLQYDWFFFGQIFSIGLLFGYLRYLSGSIWPTVILHGFNNLAATLQTLWLAAPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR44</accession>
    <name>Q3SR44_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulatory protein, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_1988</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NodD regulates the expression of the nodABCFE genes which encode other nodulation proteins. NodD is also a negative regulator of its own expression. Binds flavonoids as inducers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315234.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1988"/>
    <dbReference type="EnsemblBacteria" id="ABA05247">
        <property type="protein sequence ID" value="ABA05247"/>
        <property type="gene ID" value="Nwi_1988"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1988"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_1_5"/>
    <dbReference type="OMA" id="FMDNPLV"/>
    <dbReference type="OrthoDB" id="750230at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0536">Nodulation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="5">
        <location>
            <begin position="3"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="311" mass="34696" checksum="43D7B94F52356EEF" modified="2005-10-11" version="1">MHLTLRQLQIFDAVLQQESFTRAADALHLTQPAVSMQMRQLEEQIGVPLFDHVGKKIRPTDAGRELHRHTTLIGAKMRDLESAMEEFRGVHRGELDLTVASTANYFMPKLIAAFCACHARVQVRLQVSNQTHILETLSATNQDLAIMGQPPEDSELVARAFLDNPLMVIASPEHPLAGRRKIPLTELGCQPFITREPGSGTRNAAERHFSRLGVEFHSVMEMSSNEAIKQAVEAGLGLGILSLHTLELELAIRRLVTLDVESFPIVRQWFVVHRKGKRLSAVAQSFLTFITQQARPLASPKHPQQAPLLIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR77</accession>
    <name>Q3SR77_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF87 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1954</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1954"/>
    <dbReference type="EnsemblBacteria" id="ABA05214">
        <property type="protein sequence ID" value="ABA05214"/>
        <property type="gene ID" value="Nwi_1954"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1954"/>
    <dbReference type="eggNOG" id="COG0433">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_808526_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002789">
        <property type="entry name" value="HerA_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01935">
        <property type="entry name" value="DUF87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF87" evidence="1">
        <location>
            <begin position="180"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="343" mass="38552" checksum="0DF61A6A2F249703" modified="2005-10-11" version="1">MELFPRQQVVGIFRGFREGGMEFHADLALPYRNDFQRIPMHGHFVLVQLETPDEAVLGRIASFSSEGKLSFGSGEEFNIRAVQEQRPIPEDLREQYLKYRVNIRVLGVLRNNSSNSLTFVPSHRRLPHVGSAVAFPSGEVLKEICGHNIEGAEIGHFALGEYIYAQGSRATQIQSWMRVESPEIMVRFPVSSLVARRSFVFARAGFGKSNLNKLLFSKLYETTPTVKKRGDREEPVGTIIFDPDGEYFWPDDKGRPGLCDVPHLQDKLVVFTARAAPSAFYGSFTAGGIKLDIRQLRPSDVISIALPPERQDQQNVAKLRALSPSNWSRLKPANLRPQASIRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRM2</accession>
    <name>Q3SRM2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Deoxyguanosinetriphosphate triphosphohydrolase-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1808</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the dGTPase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315065.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1808"/>
    <dbReference type="EnsemblBacteria" id="ABA05069">
        <property type="protein sequence ID" value="ABA05069"/>
        <property type="gene ID" value="Nwi_1808"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1808"/>
    <dbReference type="eggNOG" id="COG0232">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028163_1_0_5"/>
    <dbReference type="OMA" id="HCKAFRR"/>
    <dbReference type="OrthoDB" id="370035at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016793">
        <property type="term" value="F:triphosphoric monoester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01212">
        <property type="entry name" value="dGTPase_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006261">
        <property type="entry name" value="dNTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023023">
        <property type="entry name" value="dNTPase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026875">
        <property type="entry name" value="PHydrolase_assoc_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13286">
        <property type="entry name" value="HD_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01353">
        <property type="entry name" value="dGTP_triPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="3">
        <location>
            <begin position="65"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="400" mass="44585" checksum="C0DAFFBDBD93CB59" modified="2005-10-11" version="1">MAAPPAPFASEPGRSRGRLYDEPPSRTRSAFRRDCDRVIHSTAFRRLKHKTQVFVYHEGDHFRTRLTHTLEVAQIARAMARQLGLDEDLTEALALAHDLGHPPFGHAGERALDLCLGPYGGFDHNAQTLRIVTLLEHRYPDFDGLNLTWETLEGIVKHNGPLIDAAGNAVGRYQCRGVPVGIADFNRTCDLELWSFASLEAQVAAVADDIAYDAHDIDDGLRAGLFAVDDLKEMPLTAGMIADIARRHPMLDDGRRGAELVRELISCLIMAVLSEAGRRIAGARPRSVDDVRRHSASLIAFPPEAAEAEAGIKAFLRQRMYRHPRVMRVMDEAETIVRDLFIRYRDDPAALPAEWLPEQGVNGAFDAEKARTVGNFIAGMTDRFAINEHKRLFDSTPELR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-02" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRP6</accession>
    <name>GLMU_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Bifunctional protein GlmU</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="1">UDP-N-acetylglucosamine pyrophosphorylase</fullName>
                <ecNumber evidence="1">2.7.7.23</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="1">N-acetylglucosamine-1-phosphate uridyltransferase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="1">Glucosamine-1-phosphate N-acetyltransferase</fullName>
                <ecNumber evidence="1">2.3.1.157</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="1" type="primary">glmU</name>
        <name type="ordered locus">Nwi_1784</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-acetyl-alpha-D-glucosamine 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:13725"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="ChEBI" id="CHEBI:58516"/>
            <dbReference type="EC" id="2.3.1.157"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + N-acetyl-alpha-D-glucosamine 1-phosphate + UTP = diphosphate + UDP-N-acetyl-alpha-D-glucosamine</text>
            <dbReference type="Rhea" id="RHEA:13509"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="EC" id="2.7.7.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II): step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Bacterial outer membrane biogenesis; LPS lipid A biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.23" evidence="1"/>
    <dbReference type="EC" id="2.3.1.157" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315041.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRP6"/>
    <dbReference type="SMR" id="Q3SRP6"/>
    <dbReference type="STRING" id="323098.Nwi_1784"/>
    <dbReference type="EnsemblBacteria" id="ABA05045">
        <property type="protein sequence ID" value="ABA05045"/>
        <property type="gene ID" value="Nwi_1784"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1784"/>
    <dbReference type="eggNOG" id="COG1207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_15_2_5"/>
    <dbReference type="OMA" id="TAIVEHK"/>
    <dbReference type="OrthoDB" id="1381953at2"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00532"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00533"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00973"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019134">
        <property type="term" value="F:glucosamine-1-phosphate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003977">
        <property type="term" value="F:UDP-N-acetylglucosamine diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009245">
        <property type="term" value="P:lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03353">
        <property type="entry name" value="LbH_GlmU_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01631">
        <property type="entry name" value="GlmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005882">
        <property type="entry name" value="Bifunctional_GlmU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038009">
        <property type="entry name" value="GlmU_C_LbH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01173">
        <property type="entry name" value="glmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0133">Cell shape</keyword>
    <keyword id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000233806" description="Bifunctional protein GlmU">
        <location>
            <begin position="1"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyrophosphorylase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Linker" evidence="1">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="254"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="349"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="11"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="83"/>
            <end position="84"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="352"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="366"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="372"/>
            <end position="373"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="391"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="409"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="426"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01631"/>
        </source>
    </evidence>
    <sequence length="452" mass="47148" checksum="7BADC5537064FCAB" modified="2005-10-11" version="1">MSGRNCLTIVLAAGEGTRMRSSLPKVLHPVGGQTLLSHVLAAAPGGPGTSLTVVIGPGHQAVADEAKRVRPDVMTFVQDERLGTAHAVLAAKEAIIRGADDLLIAFGDTPLISADTFARMRAPLRAGASLAVLGFHARDPAGYGRLIVTDGRLTAIREDADASPEERRMTLCNAGVMAFDGRTALRIIEKIGNANSKGEYYLTDAVGLVAELGLTAAVIETGEDEVRGINTKAQLAEAEQVMQARLRKAALDAGVTMVAPETVFLSADTSFGRDVTIEPYVIIGQGVTIADGAVVHAFSHLVQASIGRNASVGPYARLRPGTSVGDGARIGNFVETKAAVLEAGAKVNHLTYIGDAHVGAKANIGAGTITCNYDGFNKHRTEIGEGAFVGSNSSLVAPLRIGAGAYIGSGSVVTKNVPDDALAVGRGQQTLREGWAKRFREMKLLARKQKTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRP7</accession>
    <name>Q3SRP7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1783</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05044">
        <property type="protein sequence ID" value="ABA05044"/>
        <property type="gene ID" value="Nwi_1783"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1783"/>
    <dbReference type="HOGENOM" id="CLU_210354_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="58" mass="6208" checksum="808AB431C1ABE003" modified="2005-10-11" version="1">MDNEKKIKAVLMVILIIATLLGLNALIAAVQKTGNKETHTGSVLQQELTLHGEKIANA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRQ9</accession>
    <name>Q3SRQ9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF815 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1771</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315028.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1771"/>
    <dbReference type="EnsemblBacteria" id="ABA05032">
        <property type="protein sequence ID" value="ABA05032"/>
        <property type="gene ID" value="Nwi_1771"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1771"/>
    <dbReference type="eggNOG" id="COG2607">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039512_0_1_5"/>
    <dbReference type="OMA" id="NGRCAYQ"/>
    <dbReference type="OrthoDB" id="1657201at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008533">
        <property type="entry name" value="DUF815"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05673">
        <property type="entry name" value="DUF815"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF815" evidence="2">
        <location>
            <begin position="70"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="324" mass="35751" checksum="5292F184E264DF86" modified="2005-10-11" version="1">MARKSRKTSLRKPPLQTPGARSVPRAGATKGPKAAARRDAADRIARALETIAAAMTAATEQIDNHAVMTRADAFVWHPDGRLTPVSQVSRVDLTLLKGIDRMRDILMENTARFARGLPANNALLWGARGMGKSSLVKAAHASINAEAGGRLKLIEIHREDIESLPGLMELLRSSEFRFIVFCDDLSFDGNDASYKSLKAALEGGIEGRPDNVILYATSNRRHLLARDMIENERSTAINPGEAVEEKVSLSDRFGLWLGFHRCSQDEYLAMVRGYCSHFGIRLDDAELEREALEWSATRGSRSGRVAWQFTQELAGRLGVRLAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRT2</accession>
    <name>Q3SRT2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aminotransferase</fullName>
            <ecNumber evidence="2">2.6.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1748</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.6.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315005.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1748"/>
    <dbReference type="EnsemblBacteria" id="ABA05009">
        <property type="protein sequence ID" value="ABA05009"/>
        <property type="gene ID" value="Nwi_1748"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1748"/>
    <dbReference type="eggNOG" id="COG0436">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_4_3_5"/>
    <dbReference type="OMA" id="IHMEVGQ"/>
    <dbReference type="OrthoDB" id="554560at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="1">
        <location>
            <begin position="44"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="395" mass="42833" checksum="E761DA2D57F7E2B2" modified="2005-10-11" version="1">MADATPRERAASILAASSRSQVPPFMVMDVMAAAARIEAEGGHVIHMEVGQPSAPAPRTALNAARAALESGRIDYTPALGIPSLRERIARHYREFYGCTVSAERIVVTTGSSGGFILAFLSMFEPGDRVAVAVPGYPPYRHILTALGCEPVLIETSSENRHALTGEALLAAHRKTPLKGVLVGSPANPTGTMMSREALALLMAAADSEGIRFISDEIYHGLDYAFPAVTAAELSPHALIINSFSKYFCMTGWRVGWMVVPEHLVRTIERLQQNLSISVPLLSQVAAEAAFAGREEMEIVKRGYEENRNILIAGLPKAGLSEFLPADGAFYLYADVSKFTADSHDFARRMLEQARVAATPGVDFDPVRGRSFLRFSYARSAAEMHEAVARIGKWLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRY3</accession>
    <name>Q3SRY3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tad domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1697</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314956.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1697"/>
    <dbReference type="EnsemblBacteria" id="ABA04958">
        <property type="protein sequence ID" value="ABA04958"/>
        <property type="gene ID" value="Nwi_1697"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1697"/>
    <dbReference type="eggNOG" id="COG4961">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026005_0_0_5"/>
    <dbReference type="OMA" id="TIQVNTD"/>
    <dbReference type="OrthoDB" id="442586at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028087">
        <property type="entry name" value="Tad_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13400">
        <property type="entry name" value="Tad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="SSF53300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="Tad" evidence="2">
        <location>
            <begin position="24"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="605" mass="65311" checksum="CBD8190BCAB2AF57" modified="2005-10-11" version="1">MPGTSTCKRIRNSISRFDRDIRANIAPIFAIALLPVLGFVGAAVDYTRANAARSSMQAAMDSAVLMVSRDAAANPAMTSQQITDAVQRYFNSLYNDKSAFNVSVSAAYTPSTSSAAAKILASGQGAIETDFMKIAGFPQLSFGTSSTSTWGNSRMRVALVLDNTGSMRDNGKMAALQRAAKDMIDSLSAFAKTADDVYISIIPFAKDVNVDKSNYNAAWINWTEWEAEPPILIEENYPINVRYNGLTYDKWEDIGPGAPCPFDTRNNGSPRPTQNSRVGKFSFACMDRPGSLSGATDLSSLYTNRYLIPSDPSGKYYGMICPGIDGGAKFPRKRDIYYNGCYTSVVDTDRSVVVSTGSGASCPSTTPNCSCTGSGRNRKCTQAKYKHYWRAHPTDTNQAKDAAPAHSTWTGCINDRDQAYDISNADPSSGSSGTPSTKFYAEQLNGCLPATITPVSSQSSTLKNQIDSMSPSGSTNQAIGLAWGWQTLSTTNGPFPAPAKDKAYVYQDYLVLLSDGLNTRNRWSGNGSDHSPEVDVRQALLCQKVKDSGTVIFTVQVNVGNRDPLSQVLQDCASNGNFQMITSANQTADAFQNILTQISQLRIAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRY4</accession>
    <name>Q3SRY4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent Clp protease adapter protein ClpS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">clpS</name>
        <name evidence="4" type="ordered locus">Nwi_1696</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the modulation of the specificity of the ClpAP-mediated ATP-dependent protein degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Binds to the N-terminal domain of the chaperone ClpA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ClpS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1696"/>
    <dbReference type="EnsemblBacteria" id="ABA04957">
        <property type="protein sequence ID" value="ABA04957"/>
        <property type="gene ID" value="Nwi_1696"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1696"/>
    <dbReference type="eggNOG" id="COG2127">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134358_0_0_5"/>
    <dbReference type="OMA" id="WDETGRD"/>
    <dbReference type="OrthoDB" id="1829294at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00302">
        <property type="entry name" value="ClpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022935">
        <property type="entry name" value="ClpS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003769">
        <property type="entry name" value="ClpS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014719">
        <property type="entry name" value="Ribosomal_L7/12_C/ClpS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33473">
        <property type="entry name" value="PTHR33473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02617">
        <property type="entry name" value="ClpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54736">
        <property type="entry name" value="SSF54736"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ClpS" evidence="3">
        <location>
            <begin position="53"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="136" mass="15449" checksum="808BCA06CD47014F" modified="2005-10-11" version="1">MLKPPVIQTPETSVLPVSNNRSAPRMSEDEDDHAGGRGDPAASVITKVRTKTKRPNLYRVLILNDDYTPMEFVVHVLEKFFQKDLEAATQIMLHVHHHGIGECGVFTYEVAETKVTQVMDFARKHQHPLQCVMEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS00</accession>
    <name>Q3SS00_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1680</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314940.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1680"/>
    <dbReference type="EnsemblBacteria" id="ABA04941">
        <property type="protein sequence ID" value="ABA04941"/>
        <property type="gene ID" value="Nwi_1680"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1680"/>
    <dbReference type="eggNOG" id="COG0760">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049723_0_0_5"/>
    <dbReference type="OMA" id="TEYMLQQ"/>
    <dbReference type="OrthoDB" id="1838755at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027304">
        <property type="entry name" value="Trigger_fact/SurA_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109998">
        <property type="entry name" value="SSF109998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229092" evidence="1">
        <location>
            <begin position="31"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="314" mass="35381" checksum="D5916816B424B714" modified="2005-10-11" version="1">MKMTLLYRPSSLLAVFAFLTFVSMNSPLHAQSIAVMVNGEPITTLDIEQRSRLIKISTHKSPSRQEVIDELIDEKVKVKEAKKYGVNPTSTDIDRAYANMGARMGMSPEQLTKSLGSQGIRPDTIKSRLKADLVWGSLVRGRFKESLLVSDRDVNEALKNSGEDQSKIEGVEYQMRPVVLIVPRGATASMVEARRNEANALRERVQSCAEIIRTVKVMRNATLRETITKTSTDLPPPLRDLLDKTAVGHLTPPEMTRQGVEMVAVCGKKVTSVDTPKKKEIREKMFADKYEKRSKSYLERIRKSAMIEYRESRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS35</accession>
    <name>Q3SS35_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Single-stranded-DNA-specific exonuclease RecJ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_1645</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RecJ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314905.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1645"/>
    <dbReference type="EnsemblBacteria" id="ABA04906">
        <property type="protein sequence ID" value="ABA04906"/>
        <property type="gene ID" value="Nwi_1645"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1645"/>
    <dbReference type="eggNOG" id="COG0608">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009736_5_1_5"/>
    <dbReference type="OMA" id="FIICDHH"/>
    <dbReference type="OrthoDB" id="491634at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008409">
        <property type="term" value="F:5'-3' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004610">
        <property type="entry name" value="RecJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041122">
        <property type="entry name" value="RecJ_OB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17768">
        <property type="entry name" value="RecJ_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="SSF64182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00644">
        <property type="entry name" value="recJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 10" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHH" evidence="7">
        <location>
            <begin position="105"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="DHHA1" evidence="8">
        <location>
            <begin position="349"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="RecJ_OB" evidence="9">
        <location>
            <begin position="492"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="342"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA04906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="613" mass="65043" checksum="CBA86C42C0ECF7FF" modified="2005-10-11" version="1">MTLPASALPVEAPDAFLGVSHSATGRLWRDRLDPRGAARALAIAQRHQLPEMLARVIAGRGVDIDAVTDFLDPTIRRLMPDPFTVTQMETAAQRIADAAMRGETVAIFGDYDVDGATSAALLAWHLRHCGLDPLIHIPDRIFEGYGPNVDAVDALAGRGATLLVTVDCGTTSIEPLAVARQRGMSVVVIDHHQCGDDLPEVEALVNPNRPDDLSGLGHLAAVGLVFITLVALNRELRQRGFWSRERPEPNLLDMLHHVALGTVADVAPLTGLNRAFVAKGLIALRRRDHVGHTALMDVARLSGPPEAWHLGFMLGPRINAGGRIGRADLGVRLLLEGDVSEAARIAAELDRLNAERRVIEQTAEAQAEAEALASLGLEDKGSVIVTASEGWHPGVVGLVASRLKDKFARPAFVVALEPGGIGTGSGRSIGGVDLGKAVRRAVTDGVLLKGGGHAMAAGVTLRKEQLAEFRAYMETALAADVARSRHVRKLFIDGAVSARAATPEFVATLNRAGPFGAGNPEPVVALPSHQLIHADEIGQAHLRLRFRSGDGAIVNGIAFRAVGQPLGNALARMRGQPLHVAGSLAVDRWQGAERVQLRVVDVAAPDPGPKTIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS62</accession>
    <name>Q3SS62_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1618</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314883.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1618"/>
    <dbReference type="EnsemblBacteria" id="ABA04879">
        <property type="protein sequence ID" value="ABA04879"/>
        <property type="gene ID" value="Nwi_1618"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1618"/>
    <dbReference type="eggNOG" id="ENOG503376F">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_794174_0_0_5"/>
    <dbReference type="OrthoDB" id="2101756at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="349" mass="32630" checksum="F4D11E516D28BEF9" modified="2005-10-11" version="1">MFKLFDRVKVNTPTTGTGDVTFGSVSSNAFVTPAEAGAGDGDTVRYLIVDGTDFEVGIGTIKSGVTAMERTTVKESKIGGTAGTSKINLSGVAALSLTASAADILVPGNNLADLDDADEALDNLGATTVGKALFTAADAENARAALDLSDTLPTTQVFTSGAGTYTTPAGCKWIEVEMIGGGGGGAGSATSAGNGGAGGNTTFGSLTANGGAGAANAAGGAGGTASGGYFNKAGASGGHGSGLTSQWGGRGAASTFGDGGHEGQPNQAVGGTATANSGAGGGGAGCGATVNSGGGGGSGGYLRAIINNPSASYSYAVGAAGTAGTAGSGGVAGGAGGSGVIMVTEHYGP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS79</accession>
    <name>NDK_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleoside diphosphate kinase</fullName>
            <shortName evidence="1">NDK</shortName>
            <shortName evidence="1">NDP kinase</shortName>
            <ecNumber evidence="1">2.7.4.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Nucleoside-2-P kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ndk</name>
        <name type="ordered locus">Nwi_1601</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:44640"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-diphosphate + ATP = a ribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:18113"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NDK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.6" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314866.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SS79"/>
    <dbReference type="SMR" id="Q3SS79"/>
    <dbReference type="STRING" id="323098.Nwi_1601"/>
    <dbReference type="EnsemblBacteria" id="ABA04862">
        <property type="protein sequence ID" value="ABA04862"/>
        <property type="gene ID" value="Nwi_1601"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1601"/>
    <dbReference type="eggNOG" id="COG0105">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060216_8_1_5"/>
    <dbReference type="OMA" id="KIVAMKM"/>
    <dbReference type="OrthoDB" id="1752581at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006165">
        <property type="term" value="P:nucleoside diphosphate phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006228">
        <property type="term" value="P:UTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00451">
        <property type="entry name" value="NDP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001564">
        <property type="entry name" value="Nucleoside_diP_kinase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="SSF54919"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000226564" description="Nucleoside diphosphate kinase">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="1">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00451"/>
        </source>
    </evidence>
    <sequence length="140" mass="15118" checksum="29BAFE1AC1D783CA" modified="2005-10-11" version="1">MAIERTFSILKPDATARNLTGAINALIEKAGLRIVAQKRIRMTREQAETFYAVHKARPFFGELVDFMISGPVVVQVLEGEGAILKYRDIMGATDPSKAADGTIRKLHAKSVGENSVHGSDATETAAVEIAQFFSGNEIVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS90</accession>
    <name>Q3SS90_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Hemolysin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Nwi_1590</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0053 family. Hemolysin C subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1590"/>
    <dbReference type="EnsemblBacteria" id="ABA04851">
        <property type="protein sequence ID" value="ABA04851"/>
        <property type="gene ID" value="Nwi_1590"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1590"/>
    <dbReference type="eggNOG" id="COG1253">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015237_4_0_5"/>
    <dbReference type="OMA" id="YLTMEDV"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04590">
        <property type="entry name" value="CBS_pair_CorC_HlyC_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002550">
        <property type="entry name" value="CNNM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044751">
        <property type="entry name" value="Ion_transp-like_CBS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005170">
        <property type="entry name" value="Transptr-assoc_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03471">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01595">
        <property type="entry name" value="DUF21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01091">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51846">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0129">CBS domain</keyword>
    <keyword evidence="7 9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="105"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="CNNM transmembrane" evidence="12">
        <location>
            <begin position="4"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="11">
        <location>
            <begin position="228"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="11">
        <location>
            <begin position="292"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA04851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="436" mass="46816" checksum="ABF74D5B1209446D" modified="2005-10-11" version="1">MGDDSLTSLAGVLSVIILVIANGFFVASEFSLVAMRRSRVAQLVAAGGANARALQKAVEHLDYNLAACQLGITLSSLALGWIGEPALAHLIEPLLVLPFGTGAEIGAHAIAIIIAFAIITILHIVLGELAPKSLALQRTETTALIIVRPLALFGLLLKPAIVSLNGLGNGVLRLFGLSAASEKETLHSPDEIRLLVAETERAGLLARTQREVVERVISLTRRDVSDIMTPRMSIVWADADDSRDEILNVVRECKHEHVVIGQGSIDEVVGVLRKQDLLDQALEGREVDPLAVLQQPLALSEFTPILQAIERFKAQPVRVGTVVDEYGTLQGIVTRTDLLESIAGELPDAGEEPDILGGGDGKFVINGRMPIDEAMIHLGISRKPDGDFHTVAGCAIELLGRIPAVGDEFAWEGWLFRISEMDGPRISRLIASRSVE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-16" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS92</accession>
    <name>SYDND_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartate--tRNA(Asp/Asn) ligase</fullName>
            <ecNumber evidence="1">6.1.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Aspartyl-tRNA synthetase</fullName>
            <shortName evidence="1">AspRS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Non-discriminating aspartyl-tRNA synthetase</fullName>
            <shortName evidence="1">ND-AspRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aspS</name>
        <name type="ordered locus">Nwi_1588</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-aspartate + tRNA(Asx) = AMP + diphosphate + L-aspartyl-tRNA(Asx)</text>
            <dbReference type="Rhea" id="RHEA:18349"/>
            <dbReference type="Rhea" id="RHEA-COMP:9710"/>
            <dbReference type="Rhea" id="RHEA-COMP:9711"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.23"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.23" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314855.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SS92"/>
    <dbReference type="SMR" id="Q3SS92"/>
    <dbReference type="STRING" id="323098.Nwi_1588"/>
    <dbReference type="EnsemblBacteria" id="ABA04849">
        <property type="protein sequence ID" value="ABA04849"/>
        <property type="gene ID" value="Nwi_1588"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1588"/>
    <dbReference type="eggNOG" id="COG0173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014330_3_2_5"/>
    <dbReference type="OMA" id="YQLDVEM"/>
    <dbReference type="OrthoDB" id="226836at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050560">
        <property type="term" value="F:aspartate-tRNA(Asn) ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00044">
        <property type="entry name" value="Asp_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004524">
        <property type="entry name" value="Asp-tRNA-ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004115">
        <property type="entry name" value="GAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029351">
        <property type="entry name" value="GAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF5">
        <property type="entry name" value="PTHR22594:SF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02938">
        <property type="entry name" value="GAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55261">
        <property type="entry name" value="SSF55261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00459">
        <property type="entry name" value="aspS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000235535" description="Aspartate--tRNA(Asp/Asn) ligase">
        <location>
            <begin position="1"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Aspartate" evidence="1">
        <location>
            <begin position="199"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="221"/>
            <end position="223"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="450"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="484"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="491"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="536"/>
            <end position="539"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for tRNA non-discrimination" evidence="1">
        <location>
            <position position="33"/>
        </location>
    </feature>
    <feature type="site" description="Important for tRNA non-discrimination" evidence="1">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00044"/>
        </source>
    </evidence>
    <sequence length="590" mass="66704" checksum="45AC1A55C030B538" modified="2005-10-11" version="1">MHRYRSHTCGALRDSHIDQTVRLSGWCHRIRDHGGVLFIDLRDHYGLTQCVADPDSPAFALAEKLRSEWVVRIDGKARRRPAGTENPELPTGDVEIYVTEIEVLGPAAELPLPVFGEQEYPEDIRLKYRFLDLRRENLHQNIMTRGAIVDSMRKRMKEQGFFEFQTPILTASSPEGARDFLVPSRIHPGKFYALPQAPQQYKQLLMMSGFDRYFQIAPCFRDEDPRADRLPGEFYQLDLEMSFVEQDDVFAAVEPVITGVFEEFAKGKPVTRNWPRIPFAESLRKYGTDKPDLRNPLLMQDVSEHFRGSGFKVFARMLEDSKNQVWAIPGPGGGSRAFCDRMNSWAQGEGQPGLGYIMWREGGEGAGPLANNIGPERTEAIRVQLGLKAGDAAFFVAGDPAKFWKFAGLARTRLGEELNLIDKDRFELAWIVDFPMYEYNEEDKKVDFSHNPFSMPQGGLDALNTQDPLTIKAFQYDITCNGYEIASGGIRNHRPEAMVKAFEIAGYGENDVVERFGGMYRAFQYGAPPHGGMAAGVDRIVMLLCGTNNLREISLFPMNQRAEDLLMGAPSEVAPKQLRELHIRLNLPQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSD5</accession>
    <name>Q3SSD5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phage Terminase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1545</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1545"/>
    <dbReference type="EnsemblBacteria" id="ABA04806">
        <property type="protein sequence ID" value="ABA04806"/>
        <property type="gene ID" value="Nwi_1545"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1545"/>
    <dbReference type="eggNOG" id="COG4626">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026632_6_0_5"/>
    <dbReference type="OMA" id="EMMRAGT"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005021">
        <property type="entry name" value="Terminase_largesu_Lambdalike"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41287">
        <property type="entry name" value="PTHR41287"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03354">
        <property type="entry name" value="Terminase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="588" mass="66257" checksum="A4DC7043F5CAAFB4" modified="2005-10-11" version="1">MAVLAGEIVAGPLVRLACERHQRDRTSGEERGLVFDVHAAQRAIGFFRDVLTVEVEERDEYGEVSTYASPFNLQPWQAFIIGSLFGWKNALGFRRFRRAYVEIGKGNGKSPLAAGIGHYMLLGCRKLRAEVYSAATDKDQAAILFRDAVAMWERSPALRRRLKPKGANPVWELHTSRPGVNSYFKPISSDKKGKSGIRPYCALIDEVHEHPDNSVIEMMRAGTKGNQEALLFEITNSGFDKKTVCGQEHDMSVRILKREFENDAWFAFIANLDETDDPFEDESCWPKANPNLGVSIQPAFIREQVQEAQGMPSKEGLVRRLHFCQWTESATSAIPRKVWEDCEGDVDVDKLTADNVPCYGGLDLSRARDLTAFTLTWLLDATKDQWRFASKTWFWTPADTLKERAKTDRAPYELWVQKKFMEAVPGPRIRYSWVADALQQLVAKYHPVMIGADQYGLEQLLDALGEKGGTIPAEVHPQGFQRRIIGEREGDRNRETGADDIVWWMPDSINKLEAALLEQRITVAVNPVMRMCAGGVVYEQNRTGHRMFNKDKATTRIDGMVSLAMSIGVATSMRDDSSIYSKRGALVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSL5</accession>
    <name>Q3SSL5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine-type D-Ala-D-Ala carboxypeptidase</fullName>
            <ecNumber evidence="4">3.4.16.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">Nwi_1465</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the peptidase S11 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.4" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314733.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1465"/>
    <dbReference type="EnsemblBacteria" id="ABA04726">
        <property type="protein sequence ID" value="ABA04726"/>
        <property type="gene ID" value="Nwi_1465"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1465"/>
    <dbReference type="eggNOG" id="COG1686">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027070_8_1_5"/>
    <dbReference type="OMA" id="QNTHFQT"/>
    <dbReference type="OrthoDB" id="1499212at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009002">
        <property type="term" value="F:serine-type D-Ala-D-Ala carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015956">
        <property type="entry name" value="Peniciliin-bd_prot_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018044">
        <property type="entry name" value="Peptidase_S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012907">
        <property type="entry name" value="Peptidase_S11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037167">
        <property type="entry name" value="Peptidase_S11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001967">
        <property type="entry name" value="Peptidase_S11_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07943">
        <property type="entry name" value="PBP5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00768">
        <property type="entry name" value="Peptidase_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00725">
        <property type="entry name" value="DADACBPTASE1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00936">
        <property type="entry name" value="PBP5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69189">
        <property type="entry name" value="SSF69189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 16" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="9" id="KW-0133">Cell shape</keyword>
    <keyword evidence="11" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="domain" description="PBP5_C" evidence="15">
        <location>
            <begin position="297"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="12">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR618044-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR618044-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ABA04726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="413" mass="44192" checksum="39F03040BD34F643" modified="2005-10-11" version="1">MTTRSSTPDRRGGLAWRGLVAAAILTISLDGVVLAANNSVQGAKKEDIYETSAPTAILLEAKSGSVLFEKNADELRAPSSMMKLMTAEVVFNAVTKGEIKLTDEYVISENTWRKGGAPSGGSTMFAAINSRIPVQDLLRGAIIQSGNDSCMALAEGIAGHESAFAEKMTARARELGMSRSTFANSSGLPHPDNKMTVRELAALARHIILTYPDFYKLFGEREFTWNKIRQSNRNALLAAMDGADGLKTGYTKEGGYGLVGSVVQNGTRLIVVVNGLESADYRLTAARKLLEWGFRNFEVRTLFAAEQPIGYAKVFGGDSGSVQVAVSDPVQVMVQKNGGDRLIARIVYSGPVRAPIAPGQPIGVVRVWRGREVAVEMPVHATKAVGIGSTMRRAFDGVIELVVGTVRSGAAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSS3</accession>
    <name>Q3SSS3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">3-oxoacyl-[acyl-carrier-protein] synthase 3</fullName>
            <ecNumber evidence="8">2.3.1.180</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">3-oxoacyl-[acyl-carrier-protein] synthase III</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Beta-ketoacyl-ACP synthase III</fullName>
            <shortName evidence="8">KAS III</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">fabH</name>
        <name evidence="11" type="ordered locus">Nwi_1407</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched-chain and/or straight-chain of fatty acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>acetyl-CoA + H(+) + malonyl-[ACP] = 3-oxobutanoyl-[ACP] + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:12080"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9625"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78450"/>
            <dbReference type="EC" id="2.3.1.180"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The last Arg residue of the ACP-binding site is essential for the weak association between ACP/AcpP and FabH.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the thiolase-like superfamily. FabH family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.180" evidence="8"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314677.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1407"/>
    <dbReference type="EnsemblBacteria" id="ABA04668">
        <property type="protein sequence ID" value="ABA04668"/>
        <property type="gene ID" value="Nwi_1407"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1407"/>
    <dbReference type="eggNOG" id="COG0332">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039592_3_1_5"/>
    <dbReference type="OMA" id="WGSEGDK"/>
    <dbReference type="OrthoDB" id="872193at2"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033818">
        <property type="term" value="F:beta-ketoacyl-acyl-carrier-protein synthase III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01815">
        <property type="entry name" value="FabH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013751">
        <property type="entry name" value="ACP_syn_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013747">
        <property type="entry name" value="ACP_syn_III_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004655">
        <property type="entry name" value="FabH_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08545">
        <property type="entry name" value="ACP_syn_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08541">
        <property type="entry name" value="ACP_syn_III_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00747">
        <property type="entry name" value="fabH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="4 8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 8" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACP_syn_III" evidence="10">
        <location>
            <begin position="109"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="ACP_syn_III_C" evidence="9">
        <location>
            <begin position="238"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="ACP-binding" evidence="8">
        <location>
            <begin position="253"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="252"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA04668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="325" mass="34522" checksum="60E66AF3F3920DB1" modified="2005-10-11" version="1">MTAIRSVVLGCGSYLPQRVLTNSELATKVDTSDEWIVQRTGIRQRHIAAEGEFTSHLAIHAANAALAHAGIDAQSIDLIVVATSTPDHTFPATAVAVQNGLGIHHGAAFDLQAVCSGFVFALATADNFLRSGAFKRALVIGAETFSRILDWTDRSTCVLFGDGAGAVVLEAQEQPGTPLDRGILTTHLRSDGRHQSKLYVDGGPSSTRTVGHLRMEGREVFKHAVGMITDVIVDAFNASGTNANDIKWFVPHQANKRIIDASAHKLHIAPEKVVLTVDRHGNTSAASIPLALSVAVADGRIKSGDLVLLEAMGGGFTWGSALVRW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-01-10" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSV9</accession>
    <name>RL16_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplP</name>
        <name type="ordered locus">Nwi_1371</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds 23S rRNA and is also seen to make contacts with the A and possibly P site tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314646.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSV9"/>
    <dbReference type="SMR" id="Q3SSV9"/>
    <dbReference type="STRING" id="323098.Nwi_1371"/>
    <dbReference type="EnsemblBacteria" id="ABA04632">
        <property type="protein sequence ID" value="ABA04632"/>
        <property type="gene ID" value="Nwi_1371"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1371"/>
    <dbReference type="eggNOG" id="COG0197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078858_2_1_5"/>
    <dbReference type="OMA" id="KGAVEYW"/>
    <dbReference type="OrthoDB" id="1786618at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01433">
        <property type="entry name" value="Ribosomal_L16_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01342">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016180">
        <property type="entry name" value="Ribosomal_L10e/L16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036920">
        <property type="entry name" value="Ribosomal_L10e/L16_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000114">
        <property type="entry name" value="Ribosomal_L16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020798">
        <property type="entry name" value="Ribosomal_L16_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12220">
        <property type="entry name" value="PTHR12220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00252">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00060">
        <property type="entry name" value="RIBOSOMALL16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54686">
        <property type="entry name" value="SSF54686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01164">
        <property type="entry name" value="rplP_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00586">
        <property type="entry name" value="RIBOSOMAL_L16_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00701">
        <property type="entry name" value="RIBOSOMAL_L16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000062154" description="50S ribosomal protein L16">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01342"/>
        </source>
    </evidence>
    <sequence length="137" mass="15062" checksum="29EB8E25BFB5CFAE" modified="2005-10-11" version="1">MMQPKKTKFRKAHKGRIHGVASSGATLAFGQFGLKAMAPERITARQIEAARRALTRHMKRAGRVWIRVFPDVPVSKKPAEVRMGSGKGAPELWVVRVKPGRVLFEIDGVAPQTAKEALTLAAAKLPIKTRFVARIAE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSW0</accession>
    <name>RS3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsC</name>
        <name type="ordered locus">Nwi_1370</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Forms a tight complex with proteins S10 and S14.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314645.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSW0"/>
    <dbReference type="SMR" id="Q3SSW0"/>
    <dbReference type="STRING" id="323098.Nwi_1370"/>
    <dbReference type="EnsemblBacteria" id="ABA04631">
        <property type="protein sequence ID" value="ABA04631"/>
        <property type="gene ID" value="Nwi_1370"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1370"/>
    <dbReference type="eggNOG" id="COG0092">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058591_0_2_5"/>
    <dbReference type="OMA" id="KTNPIGN"/>
    <dbReference type="OrthoDB" id="1132353at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.32">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01309_B">
        <property type="entry name" value="Ribosomal_S3_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005704">
        <property type="entry name" value="Ribosomal_S3_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001351">
        <property type="entry name" value="Ribosomal_S3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036419">
        <property type="entry name" value="Ribosomal_S3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018280">
        <property type="entry name" value="Ribosomal_S3_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00189">
        <property type="entry name" value="Ribosomal_S3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54821">
        <property type="entry name" value="SSF54821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01009">
        <property type="entry name" value="rpsC_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00548">
        <property type="entry name" value="RIBOSOMAL_S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000230707" description="30S ribosomal protein S3">
        <location>
            <begin position="1"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="KH type-2" evidence="1">
        <location>
            <begin position="39"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01309"/>
        </source>
    </evidence>
    <sequence length="231" mass="26010" checksum="27D17655EC009ACA" modified="2005-10-11" version="1">MGQKINPIGLRLGINRTWDSRWYAGKAEYGRLLHEDVKIRELLHKELKQAAVARIVIERPHKKCRVTIHSARPGVVIGKKGADIDKLRKRVADITSSDVVLNIVEIRKPELDATLVAESIAQQLERRVAFRRAMKRAVQSAMRLGAEGIRINCSGRLGGAEIARMEWYREGRVPLHTLRADVDYGVATAFTTFGTCGVKVWIFKGEILEHDPMAQDKRQAGDDSRPRRDAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSX3</accession>
    <name>Q3SSX3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1357</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314636.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04618">
        <property type="protein sequence ID" value="ABA04618"/>
        <property type="gene ID" value="Nwi_1357"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1357"/>
    <dbReference type="HOGENOM" id="CLU_2618419_0_0_5"/>
    <dbReference type="OMA" id="MPRHISF"/>
    <dbReference type="OrthoDB" id="2156285at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="79" mass="8325" checksum="E3B00D712832FA05" modified="2005-10-11" version="1">MPRHISFYDESLGKQIDGSYTSDGKVIHAGSGSLGAKSATIGHLGNATTFPVDQVGQDLLAQKLLSELAHREAKNGHGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST86</accession>
    <name>Q3ST86_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Beta-ketoacyl synthase</fullName>
            <ecNumber evidence="4">2.3.1.41</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1243</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP synthases family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.41" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314534.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1243"/>
    <dbReference type="EnsemblBacteria" id="ABA04505">
        <property type="protein sequence ID" value="ABA04505"/>
        <property type="gene ID" value="Nwi_1243"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1243"/>
    <dbReference type="eggNOG" id="COG0304">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_69_2_5"/>
    <dbReference type="OMA" id="HECWMAG"/>
    <dbReference type="OrthoDB" id="606297at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00834">
        <property type="entry name" value="KAS_I_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000794">
        <property type="entry name" value="Beta-ketoacyl_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712">
        <property type="entry name" value="PTHR11712"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="B_KETOACYL_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PKS_KS" evidence="3">
        <location>
            <begin position="13"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="426" mass="44886" checksum="E369CABE3866AC5E" modified="2005-10-11" version="1">MTAIRDKSGRPVVVVTGMGVVTSLGAGKTDNWARLCAGESGIRTITRFPTEGLRTTVAGTVDFIPVEPFSSTDLSERLADIATEEAVAQAGIGRAGDFPGPLFLAVAPVELEWPQRQELGRDLGPDVDIDYDQMLRVSGGGRYKQYHRRFLFGSVADHLAETFGTKGSPVSLSTACASGTTAIQLGVEAIRRGEADAALCVATDGSVNPEALIRFSLLSALSTQNDPPHAAARPFSKNRDGFVMAEGAGALVLESYEAAAARGAKILGIVAGCGELADSFHRTRSSPDGKPIIGCVRNALADAGVTTEQIDYINAHGTGTPENDRMEYLGISSVFGERARQIPVSSNKSMVGHTLSAAGAVEAVFSLLTLEHQRIPPTINYDVPDPAIPFDVVPNTARDARVTTVMSNSFGFGGQNASLILTREPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STE1</accession>
    <name>Q3STE1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_1188</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314479.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1188"/>
    <dbReference type="EnsemblBacteria" id="ABA04450">
        <property type="protein sequence ID" value="ABA04450"/>
        <property type="gene ID" value="Nwi_1188"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1188"/>
    <dbReference type="eggNOG" id="COG0642">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_42_3_5"/>
    <dbReference type="OMA" id="FFVYDVE"/>
    <dbReference type="OrthoDB" id="692375at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="8">
        <location>
            <begin position="200"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="259"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA04450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="465" mass="50914" checksum="56D643461F3E617F" modified="2005-10-11" version="1">MRVREILGFRSNSLASRLFLSATAWVVVVLLVTGVVLSSVYRSASERAFDRRLNLYLRTLIAEVTAPDEPDDRQFQSLGEPLFDLPLSGWYWEVDRTDPGKAETRASRSLWDKKLPKLVDQGIALSSSGVRAGYVDGPEGQILRMAERPVDLGADGKFRVAVAGDASEIFDETRAFDYYLGGTFAALTIGLLLTTIFQVRFGLAPLKRISDAIADIRSGRAERLEGEFPVEIAPLAWETNALIDANREIVERARTHVGNLAHAVKTPLSVIINEASAHEGDSFAAKMLEQAGMMRNQVAHHLERARLAARLTVIGTVTDVAPVVEALRRTMEKIHRDRDITIEVDVSPGTKFRGERQDLEEMVGNLVDNACKWAHSRVFITTSVRPPSGVEPAMLHITVDDNGQGLSDAERMQASQRGRRIDETKPGSGLGLSIVTDLAALYGGRLTLDTAPIGGLRAELLLPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STG4</accession>
    <name>Q3STG4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1165</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04427">
        <property type="protein sequence ID" value="ABA04427"/>
        <property type="gene ID" value="Nwi_1165"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1165"/>
    <dbReference type="HOGENOM" id="CLU_2602460_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="79" mass="9947" checksum="888A6153F94CA034" modified="2005-10-11" version="1">MARYKIQVCIAPTDIQMYLQWRDEREYWLGRKFLAEIDDYPESQRQQDPSHIEWYYLNEDQFEKLTKFRKELRAKRKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STQ7</accession>
    <name>Q3STQ7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NAD-dependent epimerase/dehydratase</fullName>
            <ecNumber evidence="4">5.1.3.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1072</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the polysaccharide synthase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.2" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314368.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1072"/>
    <dbReference type="EnsemblBacteria" id="ABA04334">
        <property type="protein sequence ID" value="ABA04334"/>
        <property type="gene ID" value="Nwi_1072"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1072"/>
    <dbReference type="eggNOG" id="COG1086">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013560_5_0_5"/>
    <dbReference type="OMA" id="FAYRYFR"/>
    <dbReference type="OrthoDB" id="662797at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003978">
        <property type="term" value="F:UDP-glucose 4-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd05237">
        <property type="entry name" value="UDP_invert_4-6DH_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003869">
        <property type="entry name" value="Polysac_CapD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02719">
        <property type="entry name" value="Polysacc_synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="116"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Polysacc_synt_2" evidence="3">
        <location>
            <begin position="296"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="647" mass="71050" checksum="D62BE78F3767CA72" modified="2005-10-11" version="1">MNLRRKFTLRNALIALHDLVAVVAALFVSCYFRFEYSAFTARLPLLIEVLPYFLAVAAIVLYVFHLTTAKWRFFSLLDALNIVKASTLLALVILVFDYVVIAPEFYGAFFLGKKTIILYWLLQILLLAATRSTYRYFRYRRTLVHVKADDATPAIVVGRAADAEILFRSVESGAVKQLRPVGLLSPSPADIGQTIRGVSVLGGVDELEEVIADIAERQEPIKRIIMTPSAFEPDARPEAILAHGRRLGLKVSRLPSLGEGGEIPRLTPVAVEDLLLRPSRTIDYERLEGLIKNKSVVVTGGGGSIGSEICKRVVTFGASRLLIVENSEPALYAIVEELAASSRAAVVEGRVADIRDRERLMRLMNEFKPDIVFHAAALKHVPILERDWDEGVKTNIFGSVNVADAALASGAESMVMISTDKAIEPVSMLGLTKRFAEMYCQALDRDLAVRAAGKVPMRLISVRFGNVLASNGSVVPKFKAQIEAGGPVTVTHPDMVRYFMTIREACDLVITAASHAAMPERHDVSVYVLNMGQPVKIIDLAERMISLSGLQPHRDIEIVFTGVRPGERMNEILFASEEPTIEIGLPGILAAKPNEPPMETIREWIAVLKEAVASDNKLAIRSILKDAVPEFGGQPAQTSPLAPDMPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STR9</accession>
    <name>Q3STR9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Outer membrane protein assembly factor BamD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">bamD</name>
        <name evidence="3" type="ordered locus">Nwi_1060</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the Bam complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the BamD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314356.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1060"/>
    <dbReference type="EnsemblBacteria" id="ABA04322">
        <property type="protein sequence ID" value="ABA04322"/>
        <property type="gene ID" value="Nwi_1060"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1060"/>
    <dbReference type="eggNOG" id="COG4105">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065982_1_1_5"/>
    <dbReference type="OMA" id="VERQHPY"/>
    <dbReference type="OrthoDB" id="1256747at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043165">
        <property type="term" value="P:Gram-negative-bacterium-type cell outer membrane assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051205">
        <property type="term" value="P:protein insertion into membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd15830">
        <property type="entry name" value="BamD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00922">
        <property type="entry name" value="OM_assembly_BamD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017689">
        <property type="entry name" value="BamD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039565">
        <property type="entry name" value="BamD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13525">
        <property type="entry name" value="YfiO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03302">
        <property type="entry name" value="OM_YfiO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009019173" description="Outer membrane protein assembly factor BamD" evidence="1">
        <location>
            <begin position="37"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="YfiO" evidence="2">
        <location>
            <begin position="56"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="298" mass="33207" checksum="B35DA37D372335C1" modified="2005-10-11" version="1" precursor="true">MALGRRVFMDRASLGRLGRKLRLAACLALAAVTVAACGTGPLLDKFTAKDEETFSDEPADKLYNEGLFLMNKQRDLKAVTKKFDEVDREHPYSEWARKSLLMSAYASYQAGDYDTCIGSASRYVTLHPGSPDAAYAQYLIAVSNYDQIADVSRDQARTEKAMRTLEEVIRKYPTSEYAGEAKKKLQGARDQLAGKEMAVGRYYMERRDYTGAINRFKTVVTRFQTTRHVEEALARLTEAYMAIGIVAEAQTAAAVLGHNFPDSHWYKDAYNLVTSGGSQPSENKSSWISKAFRKVGLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STY6</accession>
    <name>Q3STY6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Peptidase M3B, oligoendopeptidase-related clade 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_0993</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314294.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0993"/>
    <dbReference type="EnsemblBacteria" id="ABA04255">
        <property type="protein sequence ID" value="ABA04255"/>
        <property type="gene ID" value="Nwi_0993"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0993"/>
    <dbReference type="eggNOG" id="COG1164">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021290_3_0_5"/>
    <dbReference type="OMA" id="YRQTMFA"/>
    <dbReference type="OrthoDB" id="1935578at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013647">
        <property type="entry name" value="OligopepF_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042088">
        <property type="entry name" value="OligoPept_F_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045090">
        <property type="entry name" value="Pept_M3A_M3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001567">
        <property type="entry name" value="Pept_M3A_M3B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011977">
        <property type="entry name" value="Pept_M3B_clade3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804">
        <property type="entry name" value="PTHR11804"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01432">
        <property type="entry name" value="Peptidase_M3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08439">
        <property type="entry name" value="Peptidase_M3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02290">
        <property type="entry name" value="M3_fam_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 6" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase_M3_N" evidence="9">
        <location>
            <begin position="148"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M3" evidence="8">
        <location>
            <begin position="369"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA04255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="625" mass="69694" checksum="C361021847575C43" modified="2005-10-11" version="1">MAARSSSASRKSATARKPVSRRKPARKASAAKSGAGRLPEWNLADLYAGIDAPEIARDLDRIDAECRAFEADYKGRLATETSGPEGGAWLAGAVRRYEAIDDLAGRLASFAGLIHAGDSVDPAISKFYGDVSERLTAASLHLLFFPLELNRLDDDVIERAMQTPELGHYRPWIEDSRKDKPYQLEDRLEQLFHEKSQTGYSAWNRLFDQTIAGLRFKVGAKELAIEPALNLLQDRSPEKRKAAGQALAKTFKANERTFALITNTLAKDKDISDRWRGFQDVADSRHLNNRVEREVVDALVASVRAAYPRLSHRYYALKARWFKKKKLAHWDRNAPLPFAATGVIPWGDARKMVLSAYGEFSPEMAGIAERFFTDRWIDAPVRPGKAPGAFSHPTTPSAHPYVLMNYQGKPRDVMTLAHELGHGVHQVLAAKNGALMAPTPLTLAETASVFGEMLTFKRLLAQTKNAKQRQALLAGKVEDMINTVVRQIAFYTFERAVHTERKDGELTAERLGQLWLSVQGESLGPAIEIKPGYETFWMYIPHFIHSPFYVYAYAFGDCLVNSLYAVYEHASEGFAERYLAMLAAGGTKHYSELLKPFELDARDPKFWDGGLSVIAGMIDELEGTG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STZ0</accession>
    <name>Y989_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0335 protein Nwi_0989</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">Nwi_0989</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0335 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314290.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3STZ0"/>
    <dbReference type="SMR" id="Q3STZ0"/>
    <dbReference type="STRING" id="323098.Nwi_0989"/>
    <dbReference type="EnsemblBacteria" id="ABA04251">
        <property type="protein sequence ID" value="ABA04251"/>
        <property type="gene ID" value="Nwi_0989"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0989"/>
    <dbReference type="eggNOG" id="COG3750">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158651_2_0_5"/>
    <dbReference type="OMA" id="GNGYDVK"/>
    <dbReference type="OrthoDB" id="1940079at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00797">
        <property type="entry name" value="UPF0335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018753">
        <property type="entry name" value="GapR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046367">
        <property type="entry name" value="GapR-like_DNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10073">
        <property type="entry name" value="DUF2312"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000083685" description="UPF0335 protein Nwi_0989">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00797"/>
        </source>
    </evidence>
    <sequence length="89" mass="10270" checksum="0F78EE866985EBBD" modified="2005-10-11" version="1">MVTAVAAKEEPVTSFAKDQLRAIIERIERLEEEKKTISDDIRDVYAEAKGNGYDVKALRTIVRMRKQDANERQEQETILETYMHALGML</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU14</accession>
    <name>Q3SU14_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Respiratory nitrate reductase beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0965</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314268.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0965"/>
    <dbReference type="EnsemblBacteria" id="ABA04227">
        <property type="protein sequence ID" value="ABA04227"/>
        <property type="gene ID" value="Nwi_0965"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0965"/>
    <dbReference type="eggNOG" id="COG1140">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043374_5_2_5"/>
    <dbReference type="OMA" id="CNACAHS"/>
    <dbReference type="OrthoDB" id="1762646at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009325">
        <property type="term" value="C:nitrate reductase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008940">
        <property type="term" value="F:nitrate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042126">
        <property type="term" value="P:nitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10557">
        <property type="entry name" value="NarH_beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029263">
        <property type="entry name" value="Nitr_red_bet_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038262">
        <property type="entry name" value="Nitr_red_bet_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006547">
        <property type="entry name" value="NO3_Rdtase_bsu"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13247">
        <property type="entry name" value="Fer4_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14711">
        <property type="entry name" value="Nitr_red_bet_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01660">
        <property type="entry name" value="narH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="7"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="179"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="513" mass="58000" checksum="C43937DA7B808154" modified="2005-10-11" version="1">MDIRAQVSMVFHLDKCIGCHTCSIACKNIWTDRKGTEYMYWNNVETKPGTGYPTRWEDQTKYRGGWVVDGQRQKSLRLRLQGKWGTLTNIFYNPYLPTLDDYFEPWTYDYQNLITAPLADEQPTARAISMVTGKYMDTIEAGPNWDDDLGGSQVYANNDPNFDGASDEEMRQINEINSTVFFYLPRICNHCLNPGCVAACPQGALYKRGEDGVVLVSQERCRAWRMCVSGCPYKKTYFNWSTGKAEKCILCYPRLESGQPPACFHSCVGRIRYIGLVLYDADAIEETAKSPQDQLVMAQRNIIKDPFDPEIIAAARANGIPAAKIQAAQQSPVYQFVKKWGIALPLHPEFRTLPMLFYVPPLGPVLAKVENGVYNNISHEARLGPLMSSLERSRVSLRYMASLLSGGNEEIIRDVYKKLVAVRVYMRSRKVKDIPDEEVQRALSEGKTTAAEVEAIWRLTSMPTFEERFVVPPMERETAVDALFPQLDPVSHNYPIRRGAVGVGFHTDPARGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU28</accession>
    <name>Q3SU28_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aminodeoxychorismate synthase</fullName>
            <ecNumber evidence="2">2.6.1.85</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0951</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the anthranilate synthase component I family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.85" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314254.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0951"/>
    <dbReference type="MEROPS" id="C26.959"/>
    <dbReference type="EnsemblBacteria" id="ABA04213">
        <property type="protein sequence ID" value="ABA04213"/>
        <property type="gene ID" value="Nwi_0951"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0951"/>
    <dbReference type="eggNOG" id="COG0147">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0512">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006493_0_1_5"/>
    <dbReference type="OMA" id="DWSVNIR"/>
    <dbReference type="OrthoDB" id="1786019at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046820">
        <property type="term" value="F:4-amino-4-deoxychorismate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009396">
        <property type="term" value="P:folic acid-containing compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01743">
        <property type="entry name" value="GATase1_Anthranilate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005802">
        <property type="entry name" value="ADC_synth_comp_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005801">
        <property type="entry name" value="ADC_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019999">
        <property type="entry name" value="Anth_synth_I-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006805">
        <property type="entry name" value="Anth_synth_I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015890">
        <property type="entry name" value="Chorismate_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006221">
        <property type="entry name" value="TrpG/PapA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236">
        <property type="entry name" value="PTHR11236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04715">
        <property type="entry name" value="Anth_synt_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00425">
        <property type="entry name" value="Chorismate_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56322">
        <property type="entry name" value="SSF56322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00553">
        <property type="entry name" value="pabB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00566">
        <property type="entry name" value="trpG_papA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GATase" evidence="4">
        <location>
            <begin position="5"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Anth_synt_I_N" evidence="6">
        <location>
            <begin position="239"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="Chorismate_bind" evidence="5">
        <location>
            <begin position="418"/>
            <end position="672"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="705" mass="77411" checksum="12BD2C9E92B2574E" modified="2005-10-11" version="1">MRTLIIDNYDSFTYNLVHLIADINQEDPLVVRNDALSWDELSGERFDNIVISPGPGRPDRAADFGLSKAAIESATAPLLGVCLGHQGLALAAGASLARAPSLIHGHTSRIAHQGSGLFANMPPWFNAARYHSFVVQRPLPSTLEEIAWTEDGLIMGIARHGRPQWGVQFHPESFLTEGGKVLLRNFRDLSLRFTGRTSTPPPPRTNKRRAAPPVATARKAFWFEIPRAIDTEAVFCSLFADQPFAFWLDSNLAGSPLPQWSYMGDASGPHAATAQYRSIDRQIIIEDAHGRRTENADLFEYLQREQLGRPQSAPPCPFAGGHVGWFSYELRHDCGSPTTRRAATPDALWIRPDRFIAVNHRDGTSYLCAIDSPEEAARAQHWIRSTLKRIESARRPPMNSPDAIDPNPLSFLMKDGRSDYLSKVTRCLDLIAQGETYQVCLTSELSCSATVEPLRVYRAMRSVNPAPFAAFIKWPGGAILSASPERFLAVDTEGNIEAKPIKGTIRRATDPVEDKKLAEMLRADRKNRAENGMIVDLLRHDLSRCCETGTVSVSRLFDVETYQTVHQLVSTIRGVLKPGHTLIDVLRGGAFPGGSMTGAPKFRTVELIDQLEQRARGIYSGSLGWLGDDGAADLSIVIRSIVLADGRLSIGVGGGVVAESTPEGEFEEMLLKAEASIKSIVLATFGSFSESQYRLVESGDRMAEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU53</accession>
    <name>Q3SU53_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Peptidyl-dipeptidase Dcp, Metallo peptidase, MEROPS family M03A</fullName>
            <ecNumber evidence="9">3.4.15.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_0926</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase M3 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.15.5" evidence="9"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314229.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0926"/>
    <dbReference type="EnsemblBacteria" id="ABA04188">
        <property type="protein sequence ID" value="ABA04188"/>
        <property type="gene ID" value="Nwi_0926"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0926"/>
    <dbReference type="eggNOG" id="COG0339">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001805_4_0_5"/>
    <dbReference type="OMA" id="KRSGAWC"/>
    <dbReference type="OrthoDB" id="1935578at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06456">
        <property type="entry name" value="M3A_DCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034005">
        <property type="entry name" value="M3A_DCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024077">
        <property type="entry name" value="Neurolysin/TOP_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045090">
        <property type="entry name" value="Pept_M3A_M3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001567">
        <property type="entry name" value="Pept_M3A_M3B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43660">
        <property type="entry name" value="PTHR43660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01432">
        <property type="entry name" value="Peptidase_M3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="4 7 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase_M3" evidence="8">
        <location>
            <begin position="241"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA04188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="692" mass="76993" checksum="924C9DD9D4F16E76" modified="2005-10-11" version="1">MSDTSQSSPVVSGSGTDNPLLMPWRTPFQAPPFGEIAPDHFLPAFERAFIDHAAEIAAIAHDPSAPDFANTITALERCGRLLAKVSAVFYNLVSAHSNPALLEIDKDVSSRMARHWNPILMNAVLFGRVAQLHENRATLGLTAEQSRLLERTYTRFHRAGAGLDETAKARMATINERLADLGTTFGHHLLGDEQEWFLELGEDDMDGLPDSFVAAARAAAEERALPGKAIVTTSRSSVETFLKSSRRRDLREKVYKAFTARGDNGNANDNNAAIVEILALREEAARLLGYPAFAAYRLEDSMAKTPEAARGLLERVWTPARARALADRDALQGLIAEEGGNFTLAPWDWRYYAEKLRQIRADFDDAAIKPYLTLEGMIAAAFDCAHRLFGLTFSERKDIPVWHPDVRVFEVKDEAGAHRALFYGDYFARASKRSGAWMTSLRDQQTLDGEIAPLVVNVCNFAKGADGQPALLSPDDARTLFHEFGHGLHGMLSNVTYPSLSGTSVFTDFVELPSQLYEHWQEQPQVLRQFARHYQTGEPPPDDLLERFLAARQFNQGFATVEFVASALMDLEFHSQPAAAIRDVRAFERATMGKIGMPAEIAMRHRPTQFGHIFSGDHYAAGYYSYMWSEVMDADAFGAFEETGDIFDPETARRLHDDIYSSGGSRDPEEAYIAFRGRGPKPEALLRRRGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU66</accession>
    <name>Q3SU66_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Superoxide dismutase</fullName>
            <ecNumber evidence="2 6">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_0913</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2 H(+) + 2 superoxide = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the iron/manganese superoxide dismutase family.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="2 6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314216.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0913"/>
    <dbReference type="EnsemblBacteria" id="ABA04175">
        <property type="protein sequence ID" value="ABA04175"/>
        <property type="gene ID" value="Nwi_0913"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0913"/>
    <dbReference type="eggNOG" id="COG0605">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031625_0_0_5"/>
    <dbReference type="OMA" id="KWGSFDK"/>
    <dbReference type="OrthoDB" id="1440645at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001189">
        <property type="entry name" value="Mn/Fe_SOD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019833">
        <property type="entry name" value="Mn/Fe_SOD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019832">
        <property type="entry name" value="Mn/Fe_SOD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019831">
        <property type="entry name" value="Mn/Fe_SOD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036324">
        <property type="entry name" value="Mn/Fe_SOD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036314">
        <property type="entry name" value="SOD_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02777">
        <property type="entry name" value="Sod_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00081">
        <property type="entry name" value="Sod_Fe_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000349">
        <property type="entry name" value="SODismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01703">
        <property type="entry name" value="MNSODISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46609">
        <property type="entry name" value="SSF46609"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54719">
        <property type="entry name" value="SSF54719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00088">
        <property type="entry name" value="SOD_MN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 6 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sod_Fe_N" evidence="7">
        <location>
            <begin position="2"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Sod_Fe_C" evidence="8">
        <location>
            <begin position="97"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000349-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA04175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="201" mass="22824" checksum="7BA4A7AC6188267A" modified="2005-10-11" version="1">MTFTLPNLPYSHDALAPHMSKETLEYHHDKHHQAYVTNGNNAIKGTEFEGKSLEDIVRSSYGKSASVGVFNNAGQHYNHLHFWNWMKPNGGGDKLPGRLEKKIIEDFGSLEKFKADFAAAGAGQFGSGWAWLQVKNGKLEVSKTPNAENPLIHNATPILGCDVWEHSYYIDYRNRRPDYLKAFLDHLVNWDYVDQLYSKVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU72</accession>
    <name>Q3SU72_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AAA_23 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0907</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314210.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0907"/>
    <dbReference type="EnsemblBacteria" id="ABA04169">
        <property type="protein sequence ID" value="ABA04169"/>
        <property type="gene ID" value="Nwi_0907"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0907"/>
    <dbReference type="eggNOG" id="COG0419">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015046_0_0_5"/>
    <dbReference type="OMA" id="HREIEFP"/>
    <dbReference type="OrthoDB" id="370464at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_23" evidence="3">
        <location>
            <begin position="6"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="626"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="203"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="716"/>
            <end position="746"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="873" mass="96458" checksum="E01FF1BA2004677E" modified="2005-10-11" version="1">MKISALRLFNVKRFAGRGVAIEGIDDGVNVLCAANEFGKSTSFEALHALFFQPHSSASGDVRTLRPYSGGSPLVQADIATGEGRFRITKQYFVGRSARVTDLATGQLVAQADEAENFIANLVKGGTAGPAGLLWVRQGVTAIERRSKSDDGEREVRRSLLESVQGEVEEVTGGRRMTEILSATQQALSELVTATGRPKAGGRYASALDERDKLIADEKELSDEVAALREALDQRASATKSLAELDRADDRQQRRKAVEAAQAAFDAAKSQREKLRTAEAELRLALERRGVAEKELLAFRTAMETARELRQERLAAEDRRRRAVENRREAREAIAKATAESEAAEAGERETRDLLARLDAALRAREAFERLAQLKDQLASAEATREELERGEAELSLLKLPSNAVDELQDLEIEIAKLHAVEEAARPSVAVDYQPDAIGLVTMNGDPLSDRQERTYDGQARLTIPGIGTVTLRSNRPSLSDGRLERAQAERRTLLSSMGVDDLAAARKRQVEAQKMDAELRELRGRLSQLAPDGLTRLREDVAAQSAVDSGDLELKADPAQVRATLAEAGERLKAARLALRETEPLRGRAEEAFVAAETKLAKLNAEQAQIDSILGPEEERAAREQQLVKVRDDRERSRAEAETDAKKLSDTATDLESAEAALKRARSVEEASEKEIHRLRETIAGLNAGIRARADEAVEERWRESADARSAATVRVAAFEKEIAILQRLASALEKARTEARELYLKPVMTELKPLLGLLFDDVSITFDDKSLLPQAIRRNGQEEEVDRLSGGMREQLSVLTRLAFARLLARDGRPAPVILDDALVYSDDDRIEKMFDALHRQSREQQIIVFSCRQRAFQKLGGNILQMTEWQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU94</accession>
    <name>Q3SU94_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dihydrodipicolinate synthetase</fullName>
            <ecNumber evidence="2">4.3.3.7</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0883</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DapA family.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.7" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314188.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0883"/>
    <dbReference type="EnsemblBacteria" id="ABA04147">
        <property type="protein sequence ID" value="ABA04147"/>
        <property type="gene ID" value="Nwi_0883"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0883"/>
    <dbReference type="eggNOG" id="COG0329">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049343_5_0_5"/>
    <dbReference type="OMA" id="LPPMRYK"/>
    <dbReference type="OrthoDB" id="1438588at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008840">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002220">
        <property type="entry name" value="DapA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128">
        <property type="entry name" value="PTHR12128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00701">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001365">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00146">
        <property type="entry name" value="DHPICSNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01130">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR001365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="294" mass="31365" checksum="3F7D22926A2150B4" modified="2005-10-11" version="1">MPDLAIQGIIGYTITPFDNQGRLDLNALDLTINRMIEAGVHAIAPLGSTGEGAYLSEEEWTAVAERSVKTIAGRVPVIVSVSDLTTAGAVRRARAAESFGATAVMVLPTSYWKLQEDEIVAHYQAVGTAIGVPVMLYNNHAVSGIDMSVDLILRIVDAVDNVTMVKESTGDIQRMHQLYLRTEGQLPFYNGSNPLALEAFAAGATGWCTAAANLIAADNIALYEAARQGRLVEARASFYRQLPLLDFILKGGLPKTIKAGLEIAGVPVGAPRLPVKPLDEAGRARLSAILETLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUP0</accession>
    <name>Q3SUP0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0735</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04001">
        <property type="protein sequence ID" value="ABA04001"/>
        <property type="gene ID" value="Nwi_0735"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0735"/>
    <dbReference type="eggNOG" id="ENOG5033B7M">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_365551_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="763" mass="78612" checksum="A36404CA5BA6457A" modified="2005-10-11" version="1">MIFFVFGGSVDQGYFSELCFRLLPFASPTCLDTLFLVNAALAFMFIIGGVVGACLYHLRQTRKSKATPPQKVSAGRSEAGNVFFAIFAAVGMVALLGVALTNLMQGPITSVSKVTRANIAQNDMQAAVAVITQASFVAPDADCDDDGFIEPLPFRDAEAAPAPSGGGLLPFSTAVNRRDPWGTDYGYCVWDHGGKVLDDSCGGADAMRSGGGNTQEAAAIAVMSAGPDKIFQTACEDWIDNDTPVVNKVSGSDDMWRIIPYAQMMVPASTTNARLQELPDGACNENTIGIMRLFLGTAQICLADGWVEIDDVSVESAGDFIDITGAEPASTEHLSGTISFSGFRGTRPLTVQGAAVLLINGNPVTAPAAVSDGDTIELRADAPGNPGETSTFSIMMSGIRKEWKIETRAAYPPSLSITPNAAAINVTGPGNPAYSDLTGFVVRNTGEQATGVLQAAQLVPLSNYEFSGDGDDCGGVTLEPYAKSAAQCTVDIRAKSSGEAIPSGTLTVTDGASANATATLSGTANGFNCTLAGVTVAHGASSTFYSATAHTDCASVSQTRTCNSGVLSGSGSYQYASCDTLANCTLNGVTVAHGASRTFYSATIHTNCASVSLSRTCNNGTLSGSGSYQYANCNAPANCTLGGVTVAHGASRTFYAATSHSNCAGVSQTRTCNNGTLSGSGSYQYASCTLACVPYAGQVCGYSCRNECVTACGDCAVDGQWAVNFGSLWGWICIQGSGSGYGCFPGGSGTTPTRYYACDGSCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUY9</accession>
    <name>Q3SUY9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Histone deacetylase superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0635</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the histone deacetylase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313962.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0635"/>
    <dbReference type="EnsemblBacteria" id="ABA03902">
        <property type="protein sequence ID" value="ABA03902"/>
        <property type="gene ID" value="Nwi_0635"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0635"/>
    <dbReference type="eggNOG" id="COG0123">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007727_8_1_5"/>
    <dbReference type="OMA" id="GRAMGFC"/>
    <dbReference type="OrthoDB" id="1443295at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="His_deacetylse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="SSF52768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hist_deacetyl" evidence="2">
        <location>
            <begin position="20"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="309" mass="32926" checksum="EE76C8DE616FEDA1" modified="2005-10-11" version="1">MTTLVLTHAASFDHLTPPGHPERPDRIRAIDEVLTADHFKDLARGEAPQGSLDSVALCHNEHYIAELRHLSPENGLIYLDSDTSMSPGTWEAALRGVGGAIAATEAVIAGHASNAFVATRPPGHHAETGRAMGFCLFDQAAIAARHAQRKHGIDRVAVVDFDVHHGNGTQDIFWADRSVMYCSTHQMPLFPGSGASSERGDHDTIVNAPMASGDGGARFRAAFENLILPRLRKFAPELFVISAGFDAHSRDPLATLNLAASDFEWVTRKVMDVADATAGGRIVSVLEGGYDLKALGESVAAHVAALMGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV15</accession>
    <name>Q3SV15_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0609</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313936.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0609"/>
    <dbReference type="EnsemblBacteria" id="ABA03876">
        <property type="protein sequence ID" value="ABA03876"/>
        <property type="gene ID" value="Nwi_0609"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0609"/>
    <dbReference type="HOGENOM" id="CLU_2650796_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="76" mass="8362" checksum="159683A40D8E6135" modified="2005-10-11" version="1">MRQQTGSEPSSQQLAFVMLNARPVDMDVADAESEGYYDPMSQTWIAPLHAQISSTYSTHRTAGGATSSDTTHRRDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV27</accession>
    <name>Q3SV27_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative flagellar assembly protein FliH</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0597</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313925.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0597"/>
    <dbReference type="EnsemblBacteria" id="ABA03864">
        <property type="protein sequence ID" value="ABA03864"/>
        <property type="gene ID" value="Nwi_0597"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0597"/>
    <dbReference type="eggNOG" id="COG1317">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084179_0_0_5"/>
    <dbReference type="OMA" id="ETRMETE"/>
    <dbReference type="OrthoDB" id="1950320at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018035">
        <property type="entry name" value="Flagellar_FliH/T3SS_HrpE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02108">
        <property type="entry name" value="FliH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0282">Flagellum</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FliH" evidence="2">
        <location>
            <begin position="64"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="217" mass="23506" checksum="D0CBFC7F665C810B" modified="2005-10-11" version="1">MGAPAKFLFDTDFSAPHKSRERAPTALEISQRIAAAEAAAYQKGFDAAQREARVQSDRRMAAAMEGIAANIKAVAEQFAGIEARMETEAVDVAVAVARKLCGALISAEPSADIIALVRDSVRHLVSTPHLVVRINDQLYDAARDQIERLAKQTGFTGQLVILAEPDVATGDCRIEWADGGIVLERADIDTRIDELVRGYMASRKPNSTEGMVANNER</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV60</accession>
    <name>Q3SV60_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0564</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0564"/>
    <dbReference type="EnsemblBacteria" id="ABA03831">
        <property type="protein sequence ID" value="ABA03831"/>
        <property type="gene ID" value="Nwi_0564"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0564"/>
    <dbReference type="eggNOG" id="ENOG502Z8GW">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086944_0_0_5"/>
    <dbReference type="OMA" id="WHSHWVV"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229338" evidence="1">
        <location>
            <begin position="24"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="243" mass="24956" checksum="892E3383E66DAB63" modified="2005-10-11" version="1">MPNFSTNMAIILLQGALATSALAHDIRAKPDAAVPPAFDVTSASASTDGRLATFAMEVSGDAGSLRPQKVGKLAGAKVDAYVWPTKLDPATVGFAKGSGILALAITAHPDFDDTPLFDENGDGDPTNDGADWHSHWVVLASDKACAGGLKVLDISPGQDLLPATAPGLPLALDSPGMSPILKGKTVRITVPVKGAENVNFDAVAAHLQVNETGKAPLLCVTGTYKVASGKLTLPGVITKEKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV70</accession>
    <name>Q3SV70_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">50S ribosomal subunit assembly factor BipA</fullName>
            <ecNumber evidence="3">3.6.5.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">GTP-binding protein BipA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">bipA</name>
        <name evidence="5" type="ordered locus">Nwi_0554</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">A 50S ribosomal subunit assembly protein with GTPase activity, required for 50S subunit assembly at low temperatures, may also play a role in translation. Binds GTP and analogs. Binds the 70S ribosome between the 30S and 50S subunits, in a similar position as ribosome-bound EF-G; it contacts a number of ribosomal proteins, both rRNAs and the A-site tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>GTP + H2O = GDP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="3">Binds to ribosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. BipA subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0554"/>
    <dbReference type="EnsemblBacteria" id="ABA03821">
        <property type="protein sequence ID" value="ABA03821"/>
        <property type="gene ID" value="Nwi_0554"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0554"/>
    <dbReference type="eggNOG" id="COG1217">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017016_4_0_5"/>
    <dbReference type="OMA" id="MSMLFTI"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03710">
        <property type="entry name" value="BipA_TypA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00849">
        <property type="entry name" value="BipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035651">
        <property type="entry name" value="BipA_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042116">
        <property type="entry name" value="TypA/BipA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006298">
        <property type="entry name" value="TypA_GTP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF8">
        <property type="entry name" value="PTHR42908:SF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01394">
        <property type="entry name" value="TypA_BipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Tr-type G" evidence="4">
        <location>
            <begin position="7"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="19"/>
            <end position="24"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="132"/>
            <end position="135"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="614" mass="68172" checksum="874FE6DF3DDF8D23" modified="2005-10-11" version="1">MKKNQPMNLRNVAIIAHVDHGKTTLVDRLLQQSGTYRDNQRVVERAMDSNDLERERGITILAKAASVQWNDIRINIVDTPGHADFGGEVERILNMVDGALVLVDAAEGPLPQTKFVVSKALKVGLKPIVVINKVDRPDARPTEVINEVFDLFAALDASDEQLDFPILYGSAKQGWMADSPEGSQDAGMQPLFDLIVRHVPPPQVEDGPFRMIGTIIEANPYLGRVITGRISSGMIKPNQSVKVLARDGKLIEQGRISKILAFRGLERTPLEEATAGDIVAIAGLTKGTVADTFCDPAVEEPLPAQPIDPPTVSMTFMVNNSPLAGTEGSKVTSRMIRDRLLRESEGNVALRVTEAADKDSMEVSGRGELQLAILIETMRREGFELSVSRPRVVLKKDETSGDWQEPIEEVMIDVDEEHSGVVVQKMSERKAEMMEMKPSGGNRLRLVFHAPTRGLIGYQGELLTDTRGTAIMNRLFHGYAPYKGDIQGRRNGVLISNEQGEAVAYAMFKLEDRGPMMIEPGWRVYRGMIVGEHTRDNDLEINVLKGKQLTNIRTTSKDEAVRLTPPIKMTLEKALAYIEDDELVEVTPKSIRLRKKHLDPNERKRAEKTREMVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV71</accession>
    <name>Q3SV71_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HAD-superfamily hydrolase subfamily IA, variant 3</fullName>
            <ecNumber evidence="1">3.1.3.18</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0553</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.3.18" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313881.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0553"/>
    <dbReference type="EnsemblBacteria" id="ABA03820">
        <property type="protein sequence ID" value="ABA03820"/>
        <property type="gene ID" value="Nwi_0553"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0553"/>
    <dbReference type="eggNOG" id="COG0637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_0_2_5"/>
    <dbReference type="OMA" id="KENGWPT"/>
    <dbReference type="OrthoDB" id="1167152at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008967">
        <property type="term" value="F:phosphoglycolate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044999">
        <property type="entry name" value="CbbY-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42896">
        <property type="entry name" value="PTHR42896"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="247" mass="26507" checksum="F7A9618B64230108" modified="2005-10-11" version="1">MSEASAKSLDDVRASIVQARALIFDVDGTLAETEEAHREAFNTAFAEAGLDWRWDRALYKELLRVTGGKERIRAFDVSRNGASPQLSDPEVAELHRAKTKLYAELITNGGCSLRPGVHALLAAARKRGQSLAIATTTSRANIDALLTVALGKDWAQRFVAVVAGDEVRNKKPAPDVYLKVLSELNLPGSQCLAIEDSGNGLASASAAGIPVLISRSAYFSDDDFSGAAFTIDDFTFISDEPDHNVNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVC3</accession>
    <name>Q3SVC3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chitooligosaccharide deacetylase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Nodulation protein B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_0501</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Is involved in generating a small heat-stable compound (Nod), an acylated oligomer of N-acetylglucosamine, that stimulates mitosis in various plant protoplasts.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the polysaccharide deacetylase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313829.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0501"/>
    <dbReference type="EnsemblBacteria" id="ABA03768">
        <property type="protein sequence ID" value="ABA03768"/>
        <property type="gene ID" value="Nwi_0501"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0501"/>
    <dbReference type="eggNOG" id="COG0726">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030024_1_2_5"/>
    <dbReference type="OMA" id="ATAMWNG"/>
    <dbReference type="OrthoDB" id="635734at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0536">Nodulation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NodB homology" evidence="7">
        <location>
            <begin position="92"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA03768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="352" mass="39478" checksum="7358951AB3ADD6AA" modified="2005-10-11" version="1">MKQLRNAVIRAGLDALYFSGAHHLLRPVFAGIGTIFMLHHVRPRRNSPFQPNHHLEVTPEFLRMVLAHVRSRGIDIVTADEMHRRLTQRDFSRRFACFTIDDGYRDNRDFALPAMREYDAPFTVYVANDFASATGRLWWVALERVIAAADRIDSTIGDTAITLDTSTIEQKREAFSRIHDLLRGLPDDRDVARALKDLCARHGVDESAISRELCLSWDELKPFAADPLVTIGAHTVSHCNLAKRSEEEALEEITVSRTQIEAVLQRPVVHLAYPYGDRNAAGSREFAMTRAAGFNTAVTTRPGMIFPENAEHPTALPRVSLNGNYQNARVLPVLTSGAATALWNGFRRVDIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVD3</accession>
    <name>Q3SVD3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methylase involved in ubiquinone/menaquinone biosynthesis</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0491</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313819.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0491"/>
    <dbReference type="EnsemblBacteria" id="ABA03758">
        <property type="protein sequence ID" value="ABA03758"/>
        <property type="gene ID" value="Nwi_0491"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0491"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062440_0_0_5"/>
    <dbReference type="OMA" id="CYTFFKA"/>
    <dbReference type="OrthoDB" id="1664438at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="Methyltranfer_dom" evidence="1">
        <location>
            <begin position="58"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="288" mass="31014" checksum="2AAD5F16565D7261" modified="2005-10-11" version="1">MSNPPLSEAGAATVEDQDGKARADYVLGHSDQELERLGSQGALFSDLTRDLLVRAGLKKGMRVLDVGCGVGDVSLIAAEIVSQSGSLKGIDPAAEALAIARMRLDAEGKSWASLSPGTLESLEDCSRFDAVIGRFILIHLANPAEALSRLRNRLRTGAIISFIEFDLSTAAVYPPLTLFQQCLGWICEVYRRIGRQIDMGVALFSTFRAAGLTPQMAGLTRITTANEVALDFLVDSVRSMLPAIVKLGIAAEETVGIDTLRQRLLEEVASDEYCVFYPRLVGAWATVP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-05-25" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVJ0</accession>
    <name>ATPE_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP synthase epsilon chain</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP synthase F1 sector epsilon subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">F-ATPase epsilon subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpC</name>
        <name type="ordered locus">Nwi_0434</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text>F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase epsilon chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313765.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVJ0"/>
    <dbReference type="SMR" id="Q3SVJ0"/>
    <dbReference type="STRING" id="323098.Nwi_0434"/>
    <dbReference type="EnsemblBacteria" id="ABA03701">
        <property type="protein sequence ID" value="ABA03701"/>
        <property type="gene ID" value="Nwi_0434"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0434"/>
    <dbReference type="eggNOG" id="COG0355">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084338_2_1_5"/>
    <dbReference type="OMA" id="MGGFAEI"/>
    <dbReference type="OrthoDB" id="1696893at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045261">
        <property type="term" value="C:proton-transporting ATP synthase complex, catalytic core F(1)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12152">
        <property type="entry name" value="F1-ATPase_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00530">
        <property type="entry name" value="ATP_synth_epsil_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001469">
        <property type="entry name" value="ATP_synth_F1_dsu/esu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020546">
        <property type="entry name" value="ATP_synth_F1_dsu/esu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036771">
        <property type="entry name" value="ATPsynth_dsu/esu_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13822">
        <property type="entry name" value="PTHR13822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02823">
        <property type="entry name" value="ATP-synt_DE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51344">
        <property type="entry name" value="SSF51344"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01216">
        <property type="entry name" value="ATP_synt_epsi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0139">CF(1)</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000265847" description="ATP synthase epsilon chain">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00530"/>
        </source>
    </evidence>
    <sequence length="135" mass="14276" checksum="A3A79877EB174C92" modified="2005-10-11" version="1">MATFHFDLVSPEKLAFSGEVDQVDIPGVEGDFGVLAGHAPVVAAIRPGMLTVTAGGTQQKIIVLGGLAEVSEKGLTVLADVATSIEELDRAQFADTIAGMEARLTDKEGSELDKAIERLDHFKSIQSQLNTTAMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVL4</accession>
    <name>Q3SVL4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Heat shock protein Hsp15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0410"/>
    <dbReference type="EnsemblBacteria" id="ABA03677">
        <property type="protein sequence ID" value="ABA03677"/>
        <property type="gene ID" value="Nwi_0410"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0410"/>
    <dbReference type="eggNOG" id="COG1188">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101003_3_1_5"/>
    <dbReference type="OMA" id="KWLVQAR"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="S4" evidence="1">
        <location>
            <begin position="7"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="97" mass="10797" checksum="326A63EE2E48297D" modified="2005-10-11" version="1">MDVLERQRLDKWLWHARVVRARTQAASLVEAGRVRVNGLRQTSPGHPLRIADVLTIRLDRGIRILRITGFAERRGGPTVARALYDDLSNASGEAAGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVN6</accession>
    <name>ARGJ_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Arginine biosynthesis bifunctional protein ArgJ</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="1">Glutamate N-acetyltransferase</fullName>
                <ecNumber evidence="1">2.3.1.35</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="1">Ornithine acetyltransferase</fullName>
                <shortName evidence="1">OATase</shortName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="1">Ornithine transacetylase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="1">Amino-acid acetyltransferase</fullName>
                <ecNumber evidence="1">2.3.1.1</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="1">N-acetylglutamate synthase</fullName>
                <shortName evidence="1">AGSase</shortName>
            </alternativeName>
        </domain>
        <component>
            <recommendedName>
                <fullName evidence="1">Arginine biosynthesis bifunctional protein ArgJ alpha chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="1">Arginine biosynthesis bifunctional protein ArgJ beta chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="1" type="primary">argJ</name>
        <name type="ordered locus">Nwi_0388</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N(2)-acetylornithine and glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamate + N(2)-acetyl-L-ornithine = L-ornithine + N-acetyl-L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:15349"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
            <dbReference type="EC" id="2.3.1.35"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + L-glutamate = CoA + H(+) + N-acetyl-L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text>Some bacteria possess a monofunctional ArgJ, i.e. capable of catalyzing only the fifth step of the arginine biosynthetic pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ArgJ family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.35" evidence="1"/>
    <dbReference type="EC" id="2.3.1.1" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313719.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVN6"/>
    <dbReference type="SMR" id="Q3SVN6"/>
    <dbReference type="STRING" id="323098.Nwi_0388"/>
    <dbReference type="MEROPS" id="T05.001"/>
    <dbReference type="EnsemblBacteria" id="ABA03655">
        <property type="protein sequence ID" value="ABA03655"/>
        <property type="gene ID" value="Nwi_0388"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0388"/>
    <dbReference type="eggNOG" id="COG1364">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027172_1_0_5"/>
    <dbReference type="OMA" id="DYVHENS"/>
    <dbReference type="OrthoDB" id="1083409at2"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00106"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00111"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004042">
        <property type="term" value="F:acetyl-CoA:L-glutamate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004358">
        <property type="term" value="F:glutamate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103045">
        <property type="term" value="F:methione N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02152">
        <property type="entry name" value="OAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01106">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002813">
        <property type="entry name" value="Arg_biosynth_ArgJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016117">
        <property type="entry name" value="ArgJ-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042195">
        <property type="entry name" value="ArgJ_beta_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23100">
        <property type="entry name" value="PTHR23100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01960">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56266">
        <property type="entry name" value="SSF56266"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00120">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000227236" description="Arginine biosynthesis bifunctional protein ArgJ alpha chain" evidence="1">
        <location>
            <begin position="1"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000227237" description="Arginine biosynthesis bifunctional protein ArgJ beta chain" evidence="1">
        <location>
            <begin position="199"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="412"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="417"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" evidence="1">
        <location>
            <position position="125"/>
        </location>
    </feature>
    <feature type="site" description="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" evidence="1">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="1">
        <location>
            <begin position="198"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01106"/>
        </source>
    </evidence>
    <sequence length="417" mass="43321" checksum="91D8E41E5370DC74" modified="2005-10-11" version="1">MSTAISPLAPTDVPDLPEIAGVRLATAAAGIRYKGRTDVLLALLDEGTTVAGVFTRSRCPSAPVEWCRARLKDGHHAKAGLALVVNSGNANAFTGKTGRQATKLTAGIAAKAAGCKASDIYLASTGVIGEPLDATKFNGVLETLASEAAPDRWMDAARAIMTTDTFPKVATARVKLGKAAVTINGIAKGAGMIAPDMATMLSFIFTDAPITATALQSLLKSGVEDTFNAVTIDSDTSTSDTLLAFATGAAGARGAPRISRAGDPRLKAFVKAFHGVLADLAEQVARDGEGARKLVEVIVEGATSKASARKIAKSIANSPLVKTAIAGEDANWGRVVMAVGKAGEPADRDKLSIYFNGIRVAKSGARDPSYDEAEVSKAMKNDRIQIKTTLGLGKGRDRVLTCDLTKEYVAINGDYRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVN9</accession>
    <name>Q3SVN9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphoribosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0385</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313716.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0385"/>
    <dbReference type="EnsemblBacteria" id="ABA03652">
        <property type="protein sequence ID" value="ABA03652"/>
        <property type="gene ID" value="Nwi_0385"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0385"/>
    <dbReference type="eggNOG" id="COG1040">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054549_0_0_5"/>
    <dbReference type="OMA" id="RYNQSAL"/>
    <dbReference type="OrthoDB" id="1937725at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044005">
        <property type="entry name" value="DZR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18912">
        <property type="entry name" value="DZR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DZR_2" evidence="2">
        <location>
            <begin position="35"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Pribosyltran" evidence="1">
        <location>
            <begin position="178"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="271" mass="29994" checksum="6B040D4E872DE0B2" modified="2005-10-11" version="1">MELSTDNLFSPHPARLLRGAWRACRETMSYAARTALDIALPTLCVACREPVAGVGVCADCWTKLSFIERPYCPRLGTPFVYDPGSEMLSMEAIANPPAYQRARAAVRYDDVAKVLVHALKYQDRTDLAPVMGRWMARAGSGLLDGADLLIPVPLHWRRGWSRRFNQSGMLARVIARHSGVPVAPDTLRRIRPTRQQVRLSRNDRARNVQGAFKVTAERAGHVQGRRVILIDDVLTSGATVDACARVLLRAKAAQVDVLVFARVVDTMKAPI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVQ7</accession>
    <name>RECR_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Recombination protein RecR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">recR</name>
        <name type="ordered locus">Nwi_0367</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May play a role in DNA repair. It seems to be involved in an RecBC-independent recombinational process of DNA repair. It may act with RecF and RecO.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RecR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313698.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVQ7"/>
    <dbReference type="SMR" id="Q3SVQ7"/>
    <dbReference type="STRING" id="323098.Nwi_0367"/>
    <dbReference type="EnsemblBacteria" id="ABA03634">
        <property type="protein sequence ID" value="ABA03634"/>
        <property type="gene ID" value="Nwi_0367"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0367"/>
    <dbReference type="eggNOG" id="COG0353">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060739_1_1_5"/>
    <dbReference type="OMA" id="DVMAIEN"/>
    <dbReference type="OrthoDB" id="1661836at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01025">
        <property type="entry name" value="TOPRIM_recR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00017">
        <property type="entry name" value="RecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000093">
        <property type="entry name" value="DNA_Rcmb_RecR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023627">
        <property type="entry name" value="Rcmb_RecR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023628">
        <property type="entry name" value="Rcmb_RecR_C4-type_Zn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015967">
        <property type="entry name" value="Rcmb_RecR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034137">
        <property type="entry name" value="TOPRIM_RecR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30446">
        <property type="entry name" value="PTHR30446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02132">
        <property type="entry name" value="RecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13662">
        <property type="entry name" value="Toprim_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111304">
        <property type="entry name" value="SSF111304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00615">
        <property type="entry name" value="recR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01300">
        <property type="entry name" value="RECR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_1000057155" description="Recombination protein RecR">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Toprim" evidence="1">
        <location>
            <begin position="83"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="1">
        <location>
            <begin position="60"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00017"/>
        </source>
    </evidence>
    <sequence length="201" mass="21430" checksum="42F5B43A6BD8B233" modified="2005-10-11" version="1">MPTAVTGPEIERLIQLLARLPGLGPRSARRAALHLIKKREALMAPLASALQVAIDRIRVCETCGNIDTRSPCTICTDARRDPSIIVVVADVADLWALERAGATNGFYHVLGATLSPLDGVGPQDLTIDALVARAHDPRVAEIVLALNATVDGQTTAHYITDLLGEANVKVTRLAHGVPVGGELDYLDEGTLSAAMRQRTLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVT4</accession>
    <name>Q3SVT4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Molybdenum ABC transporter, periplasmic binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0340</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein ModA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313672.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0340"/>
    <dbReference type="EnsemblBacteria" id="ABA03607">
        <property type="protein sequence ID" value="ABA03607"/>
        <property type="gene ID" value="Nwi_0340"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0340"/>
    <dbReference type="eggNOG" id="COG0725">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065520_3_0_5"/>
    <dbReference type="OMA" id="VCAPQVP"/>
    <dbReference type="OrthoDB" id="1506401at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015689">
        <property type="term" value="P:molybdate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005950">
        <property type="entry name" value="ModA"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004846">
        <property type="entry name" value="ModA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01256">
        <property type="entry name" value="modA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229290" evidence="4">
        <location>
            <begin position="25"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>molybdate</name>
            <dbReference type="ChEBI" id="CHEBI:36264"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>molybdate</name>
            <dbReference type="ChEBI" id="CHEBI:36264"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>molybdate</name>
            <dbReference type="ChEBI" id="CHEBI:36264"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>molybdate</name>
            <dbReference type="ChEBI" id="CHEBI:36264"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR004846-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="274" mass="28533" checksum="2B7D92E9AF87D619" modified="2005-10-11" version="1">MKSVTRSLLAALLVGATFALPASAKDARPQATTPPAAQARTLTVFAAASLKNALDEVDYAYAAKTGVKITASYAASSALAKQIEQGAPADVYVSADVAWMDYVNSKKAINEPTRVNLLGNSIVLIAPKDSKIDNVTIDQGFDLAQLAGDGKIATGDVKAVPVGKYAKAALEKLGAWTAVEPKVAMAENVRAALALVARNEAVLGIVYSTDARVEPGVKVVGTFPASSHPAIIYPVAATSTAHKDTADYLAFLRGSEAKAIFEKHGFTFLVDPTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVU3</accession>
    <name>Q3SVU3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Perplasmic binding protein of ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0331</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313663.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0331"/>
    <dbReference type="EnsemblBacteria" id="ABA03598">
        <property type="protein sequence ID" value="ABA03598"/>
        <property type="gene ID" value="Nwi_0331"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0331"/>
    <dbReference type="eggNOG" id="COG0715">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028871_1_4_5"/>
    <dbReference type="OMA" id="AMQGYVT"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027939">
        <property type="entry name" value="NMT1/THI5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015168">
        <property type="entry name" value="SsuA/THI5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31528">
        <property type="entry name" value="PTHR31528"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09084">
        <property type="entry name" value="NMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229291" evidence="1">
        <location>
            <begin position="26"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="NMT1" evidence="2">
        <location>
            <begin position="44"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="344" mass="37851" checksum="645D3F8267C95D43" modified="2005-10-11" version="1">MSLAFLLRRLTVAIGLALGVASSAAAEPALDRVSFGTNWVAEGEHGGFFQALADGTYRKFGLDVTIVPGGPNVNNRVLLIAGKLDFFMTANTLQSFDAVANNVPVVAVAAMFQKDPQVFLAHPESKVTTIEDLKPLNLFISKEGIASYFQWLKSEYGFSAGKIRPYTFNAQPFLADKKSAMQGYVTSEPFAVERQAGFKPTVILLADAGFNPYSTLIETRRDLVETKQDLVQRFVDASIIGWYTYLYGDNTAGNAMIKKLNPEMTDDLLAYSVAKMKEHGIVDSGDSLRDGIGAMTDVRYAGLFDQMVRAGVVRRDLDYRKAYTLQFVNKGVGLDLRPRNLQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVW7</accession>
    <name>Q3SVW7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flp pilus assembly protein TadG</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0307</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0307"/>
    <dbReference type="EnsemblBacteria" id="ABA03574">
        <property type="protein sequence ID" value="ABA03574"/>
        <property type="gene ID" value="Nwi_0307"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0307"/>
    <dbReference type="eggNOG" id="COG4961">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108008_1_0_5"/>
    <dbReference type="OMA" id="WTKSDVA"/>
    <dbReference type="OrthoDB" id="1627553at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012495">
        <property type="entry name" value="TadE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07811">
        <property type="entry name" value="TadE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="242" mass="25891" checksum="63C2FBBAF915053A" modified="2005-10-11" version="1">MRIENVKPMWGACLSEVDAGSREESASQRGSGASLAFYRNGNGSGMWSCLKDMASALRRDTRGLAATEFAMIVPLMLVMLFGTIEVSSGVAVNRKVTLVARTLSDLTSQSKVVNDADVTNFLAASYGIMWPYSSAPVQATISELYIDPATSVARVQWSKGKAPRGAGSTVSIPSGLIGRDSSGKVLPNQYLIFSEVSYIYEPVLGYVMSKAGIRLSDTAFTRPRQSACVFFGNPSSIICPTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW13</accession>
    <name>Q3SW13_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0261</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0261"/>
    <dbReference type="EnsemblBacteria" id="ABA03528">
        <property type="protein sequence ID" value="ABA03528"/>
        <property type="gene ID" value="Nwi_0261"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0261"/>
    <dbReference type="eggNOG" id="COG2253">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092368_0_0_5"/>
    <dbReference type="OMA" id="QDLIICR"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014942">
        <property type="entry name" value="AbiEii"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08843">
        <property type="entry name" value="AbiEii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="271" mass="30513" checksum="FE722CCC0E735C14" modified="2005-10-11" version="1">MIPRDYITEWRAQAPWVQDIQVEQDLVICRALVEIFSHPVLSQALAFRGGTALYKLYLKPAARYSEDIDLVQMRAEPAGPVMTALREVLDPWLGAPRWKQTEGRVTFVYRFMSEDNPPIGMRLKVEINSREHFAVHGFTRIPFSVASRWFEGACEIPTYELDELLATKLRALYQRRKGRDLFDLETALKVDGVDAGRIVEAFGAYMDHGGHQVTRAQFERNMAQKMGDPQFTADIGPLLAGDHEWDIEAAAAAVGGNLIARLPGPPSDDRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW22</accession>
    <name>Q3SW22_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulatory protein, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_0252</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NodD regulates the expression of the nodABCFE genes which encode other nodulation proteins. NodD is also a negative regulator of its own expression. Binds flavonoids as inducers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0252"/>
    <dbReference type="EnsemblBacteria" id="ABA03519">
        <property type="protein sequence ID" value="ABA03519"/>
        <property type="gene ID" value="Nwi_0252"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0252"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_32_2_5"/>
    <dbReference type="OMA" id="WEKRTGP"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0536">Nodulation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA03519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="216" mass="23641" checksum="B6D09E5ABA29BD94" modified="2005-10-11" version="1">MDLRHLRYIVAAARNGSFSAAANEFNARQPIVSKRIKEVEDELGVRLFERSTAGARLTLLGEDFVVDARRVLDETEHMARHAKASRAGTLGHVVVGFYKSLSAGAFRAAVSCFRKQNPDIDIELVELPFADIAAGVISGALDAAIVLGDAGKCAMFKTIGLWSEHLVIALPKDHPLADKPIVYWPDLRGERFLVTVRDPGPDTRSTRRRNAGLPGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW23</accession>
    <name>Q3SW23_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Superfamily II DNA/RNA helicase, SNF2 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0251</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0251"/>
    <dbReference type="EnsemblBacteria" id="ABA03518">
        <property type="protein sequence ID" value="ABA03518"/>
        <property type="gene ID" value="Nwi_0251"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0251"/>
    <dbReference type="eggNOG" id="COG0553">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037004_0_0_5"/>
    <dbReference type="OMA" id="YVWEQCD"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 5" id="KW-0347">Helicase</keyword>
    <keyword evidence="1 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="3">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="4">
        <location>
            <begin position="301"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="506"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="520"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="541" mass="60353" checksum="8B4604C672EFDDCA" modified="2005-10-11" version="1">MPVTLLADDVGLGKTISAGLVASELIARNRVAKILIVAPKLLGPQWKDELEAKFDIPGEVVTGSKLASAEPDEVGAVITTYHSARAHLDKIPDDRFDMLVLDEAHKLRNLYGVDPTPQVAKVFHRTLQQRRFRYVLMLTATPIQNRLWDLYSLVDLLTVARGHENPFGSEGMFARRFIADKRETARILKPDAREDFRKIVYGYMSRVRRGDANLDFPERKIQLHKVAPSSEELALIKAIAKPIQKLNRLAQISILQALTSSPHALMVQLQTMARNGTVPMELAATVQAIVTPMRTSAKLEGLRQLVSMLQQQNPTSWRMVVFTGRRETQTTIQAFLEELGLKVGIINGSSGQRNQDTIARFRGNPPEIRAIVSTEAGSEGVNLQIANVLINFDLPWNPMIVEQRIGRVQRLGSQHKNVVIYNVMLAGTFEEYIVGRLMTKLQMATDAIGDIESLLEASGVGDGEEGTGFDEKIRELVVAASMLPRRPLKKKTRISTPPLELWMATNMSAHKRRRSKRPNPPWNSNHSSARPLSTLAARLQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWC1</accession>
    <name>Q3SWC1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ErfK/YbiS/YcfS/YnhG</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0152</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YkuD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313489.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0152"/>
    <dbReference type="MEROPS" id="C82.003"/>
    <dbReference type="EnsemblBacteria" id="ABA03420">
        <property type="protein sequence ID" value="ABA03420"/>
        <property type="gene ID" value="Nwi_0152"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0152"/>
    <dbReference type="eggNOG" id="COG1376">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042399_2_1_5"/>
    <dbReference type="OMA" id="YREPAWA"/>
    <dbReference type="OrthoDB" id="975147at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071972">
        <property type="term" value="F:peptidoglycan L,D-transpeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16913">
        <property type="entry name" value="YkuD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005490">
        <property type="entry name" value="LD_TPept_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038063">
        <property type="entry name" value="Transpep_catalytic_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03734">
        <property type="entry name" value="YkuD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141523">
        <property type="entry name" value="SSF141523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="YkuD" evidence="4">
        <location>
            <begin position="40"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="172" mass="18272" checksum="B39F908C6A8DFD90" modified="2005-10-11" version="1">MLTRVAAVLAAIWGAGLLGIGSAEARPEMVGYQGGYAPGTIVVKTSERRLYLIIDSDRALRYPVGVGRAGKQWSGAARIEGKYRNPAWAPPAEVKRDNPRIADVIPGGSPANPMGVAAMTLSGGGQYAIHGTNRPGSIGRFVSYGCIRMYNADIDDLYRRVSVGTPVVVTQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWE2</accession>
    <name>Q3SWE2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0131</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313468.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03399">
        <property type="protein sequence ID" value="ABA03399"/>
        <property type="gene ID" value="Nwi_0131"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0131"/>
    <dbReference type="eggNOG" id="COG1451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065947_2_1_5"/>
    <dbReference type="OMA" id="IFCPNYR"/>
    <dbReference type="OrthoDB" id="1301886at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002725">
        <property type="entry name" value="YgjP-like_metallopeptidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01863">
        <property type="entry name" value="DUF45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF45" evidence="1">
        <location>
            <begin position="66"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="278" mass="31991" checksum="84931425BCFC64EF" modified="2005-10-11" version="1">MICFCAERFEWRRIWQNPGGISPSGFADMARALLYRRPAEPSRLLIKHGSQIYSVRLRRHRRARRYTLRIHPSDREAILTMPPRGTLAEAKDFAQRHGGWIAARLGRLPKAAPFQHGAVIPLRGVAHRIVHRAGERGTVWTETRDSGERILCVAGGLEYLDRRVHDFLKREARQDLQKASQRYADALGVKVRRISIRDQSSRWGSCTSAGSLSFSWRLILAPPFVLDYLAAHEVAHLVEMNHSPRFWKVVARVCKHVERAKKWLDVEGNDLHRYGIQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWJ5</accession>
    <name>Q3SWJ5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acyl-CoA thioesterase</fullName>
            <ecNumber evidence="3">3.1.2.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0078</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the C/M/P thioester hydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.2" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313415.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0078"/>
    <dbReference type="EnsemblBacteria" id="ABA03346">
        <property type="protein sequence ID" value="ABA03346"/>
        <property type="gene ID" value="Nwi_0078"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0078"/>
    <dbReference type="eggNOG" id="COG1946">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032690_0_0_5"/>
    <dbReference type="OMA" id="SQVWFRT"/>
    <dbReference type="OrthoDB" id="990202at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102991">
        <property type="term" value="F:myristoyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016290">
        <property type="term" value="F:palmitoyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042171">
        <property type="entry name" value="Acyl-CoA_hotdog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003703">
        <property type="entry name" value="Acyl_CoA_thio"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11066">
        <property type="entry name" value="PTHR11066"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00189">
        <property type="entry name" value="tesB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="300" mass="33913" checksum="B858A30D1B1419CB" modified="2005-10-11" version="1">MSESLTDLISILDLEPIEENLFRGGSPKTGWQRVFGGQVIGQAMAAACRTVEGRLPHSLHCYFILPGDPAVPIIYQVERLRDGRSYSTRRVTAIQHGRAIFSIMVSFHVDEEGAFNHQEKMPDVPPPEQLSPEEFAKQPTFKEMPEFIRRYYESDRPIELRPVELNRYFGERIEDGRVHVWIRTASNLPDDPALHMCALAYASDFALLDAVMARYGRTLFDKHMLAASLDHAMWFHRPFRADEWLLYAQDSPSAQSGRGLARGLIFRQDGTLIASVAQEGSVRERSSSIFASDGIRSEAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWN2</accession>
    <name>Q3SWN2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE</fullName>
            <ecNumber evidence="6">2.1.1.163</ecNumber>
            <ecNumber evidence="6">2.1.1.201</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">2-methoxy-6-polyprenyl-1,4-benzoquinol methylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Demethylmenaquinone methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ubiE</name>
        <name evidence="7" type="ordered locus">Nwi_0041</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Methyltransferase required for the conversion of demethylmenaquinol (DMKH2) to menaquinol (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 2-demethylmenaquinol + S-adenosyl-L-methionine = a menaquinol + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42640"/>
            <dbReference type="Rhea" id="RHEA-COMP:9539"/>
            <dbReference type="Rhea" id="RHEA-COMP:9563"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18151"/>
            <dbReference type="ChEBI" id="CHEBI:55437"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.1.1.163"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 2-methoxy-6-all-trans-polyprenyl-1,4-benzoquinol + S-adenosyl-L-methionine = a 6-methoxy-3-methyl-2-all-trans-polyprenyl-1,4-benzoquinol + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:28286"/>
            <dbReference type="Rhea" id="RHEA-COMP:10858"/>
            <dbReference type="Rhea" id="RHEA-COMP:10859"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:84166"/>
            <dbReference type="ChEBI" id="CHEBI:84167"/>
            <dbReference type="EC" id="2.1.1.201"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Quinol/quinone metabolism; menaquinone biosynthesis; menaquinol from 1,4-dihydroxy-2-naphthoate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class I-like SAM-binding methyltransferase superfamily. MenG/UbiE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.163" evidence="6"/>
    <dbReference type="EC" id="2.1.1.201" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0041"/>
    <dbReference type="EnsemblBacteria" id="ABA03309">
        <property type="protein sequence ID" value="ABA03309"/>
        <property type="gene ID" value="Nwi_0041"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0041"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_0_1_5"/>
    <dbReference type="OMA" id="VRNFENL"/>
    <dbReference type="UniPathway" id="UPA00079">
        <property type="reaction ID" value="UER00169"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043333">
        <property type="term" value="F:2-octaprenyl-6-methoxy-1,4-benzoquinone methylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043770">
        <property type="term" value="F:demethylmenaquinone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102094">
        <property type="term" value="F:S-adenosylmethionine:2-demethylmenaquinol methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102955">
        <property type="term" value="F:S-adenosylmethionine:2-demethylmenaquinol-7 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102027">
        <property type="term" value="F:S-adenosylmethionine:2-demethylquinol-8 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01813">
        <property type="entry name" value="MenG_UbiE_methyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004033">
        <property type="entry name" value="UbiE/COQ5_MeTrFase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023576">
        <property type="entry name" value="UbiE/COQ5_MeTrFase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01209">
        <property type="entry name" value="Ubie_methyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01934">
        <property type="entry name" value="MenG_MenH_UbiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51608">
        <property type="entry name" value="SAM_MT_UBIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01183">
        <property type="entry name" value="UBIE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01184">
        <property type="entry name" value="UBIE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0474">Menaquinone biosynthesis</keyword>
    <keyword evidence="2 6 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 6" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="6">
        <location>
            <begin position="137"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="265" mass="29265" checksum="8366650D9B74713D" modified="2005-10-11" version="1">MAGYGPVMEKTGMDREDQTTHFGFRDVPLGDKQTLVNDVFHSVARRYDLMNDLMSGGLHRVWKDAMITTLNPPRNDAPFSLLDVAGGTGDIAFRAAKAAGSGFQATVCDINPDMLAVGRERATKQHRDHQVSFVEGNAETLAFADRGFDAYTIAFGIRNVPRIDAALREAFRVLRPGGRFLCLEFSTVDVPGLDKIYDLFSFKMIPPLGRAVTGDADSYRYLVESIRKFPKPNAFAEMIRDAGFARVSWQALSGGIVALHSGWRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWQ5</accession>
    <name>Q3SWQ5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Apolipoprotein N-acyltransferase</fullName>
            <shortName evidence="9">ALP N-acyltransferase</shortName>
            <ecNumber evidence="9">2.3.1.269</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">lnt</name>
        <name evidence="11" type="ordered locus">Nwi_0018</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the phospholipid dependent N-acylation of the N-terminal cysteine of apolipoprotein, the last step in lipoprotein maturation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a glycerophospholipid + N-terminal S-1,2-diacyl-sn-glyceryl-L-cysteinyl-[lipoprotein] = a 2-acyl-sn-glycero-3-phospholipid + H(+) + N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteinyl-[lipoprotein]</text>
            <dbReference type="Rhea" id="RHEA:48228"/>
            <dbReference type="Rhea" id="RHEA-COMP:14681"/>
            <dbReference type="Rhea" id="RHEA-COMP:14684"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136912"/>
            <dbReference type="ChEBI" id="CHEBI:140656"/>
            <dbReference type="ChEBI" id="CHEBI:140657"/>
            <dbReference type="ChEBI" id="CHEBI:140660"/>
            <dbReference type="EC" id="2.3.1.269"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Protein modification; lipoprotein biosynthesis (N-acyl transfer).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell inner membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the CN hydrolase family. Apolipoprotein N-acyltransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.269" evidence="9"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0018"/>
    <dbReference type="EnsemblBacteria" id="ABA03286">
        <property type="protein sequence ID" value="ABA03286"/>
        <property type="gene ID" value="Nwi_0018"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0018"/>
    <dbReference type="eggNOG" id="COG0815">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019563_3_1_5"/>
    <dbReference type="OMA" id="IPQSMKW"/>
    <dbReference type="UniPathway" id="UPA00666"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016410">
        <property type="term" value="F:N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042158">
        <property type="term" value="P:lipoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07571">
        <property type="entry name" value="ALP_N-acyl_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01148">
        <property type="entry name" value="Lnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004563">
        <property type="entry name" value="Apolipo_AcylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045378">
        <property type="entry name" value="LNT_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20154">
        <property type="entry name" value="LNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="SSF56317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00546">
        <property type="entry name" value="lnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="42"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="104"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="213"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="509"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="CN hydrolase" evidence="10">
        <location>
            <begin position="249"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA03286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="535" mass="57568" checksum="6E03B2DC6EEE5209" modified="2005-10-11" version="1">MSLSIAGSARTAGVAVTLSWGWKRAFVALASGAASSLAMEPFNAWPLLFVTFPVATWLIDGSAGRLRGVPAAALAGWLFGFGYFVPGLYWIGNAFLVDAQTFAWLIPFAVLGLPAYLALFTALGFALARFIWTTDASRILALAAALTISEWLRGHVLTGFPWNAFGYALTEPLALAQTASLIGLWGLTFIGVAIFASPAVLIDGGSRGRRPWLVPVTALMLLLAMSIYGSVRLSRHPTEFADNVKLRIMQPNLAQDARFNYSAKAAVMQKYLALSDRATGPHSTGVSGATILIWPESAFPFFLTREADAMAEIADLLPKGTILMTGSVRAPDPSPNGRITRAFNSIYVIGDDGGILSIYDKLHLVPFGEFLPFQGLMEKLGFVQLTKVQGGFIPGKSRRTIELPNAPSVLPLICYEAIFPDGMQTRGGRPGWIVNLTNDGWFGISTGPHQHLQQARLRAIEQGLPLVRAANTGISVVTDPVGRIVARLDLGVESVLDSRLPVNMTPTPYARAGDIPAAIMVTMALVLVIRRRRGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMV6</accession>
    <name>Q3SMV6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Resolvase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_3138</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3138"/>
    <dbReference type="EnsemblBacteria" id="ABA06385">
        <property type="protein sequence ID" value="ABA06385"/>
        <property type="gene ID" value="Nwi_3138"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3138"/>
    <dbReference type="eggNOG" id="COG1961">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010686_18_11_5"/>
    <dbReference type="OMA" id="HDVSIPD"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038109">
        <property type="entry name" value="DNA_bind_recomb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011109">
        <property type="entry name" value="DNA_bind_recombinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025827">
        <property type="entry name" value="Zn_ribbon_recom_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07508">
        <property type="entry name" value="Recombinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13408">
        <property type="entry name" value="Zn_ribbon_recom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="SSF53041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51737">
        <property type="entry name" value="RECOMBINASE_DNA_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="3">
        <location>
            <begin position="24"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Recombinase" evidence="4">
        <location>
            <begin position="182"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="541"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="411"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA06385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="561" mass="63218" checksum="4B807A0CBAA9F98D" modified="2005-10-11" version="1">MAASEIGISMSVHKAAQTLPSMHRAALYMRVSTGRQAEHDLSIPDQRHQLQSWCRAHGYEPAAEFVEAGASATDDRRPIFQQMIERACDGENAFDVIIVHSYSRFFREAFEQEFYLRKLARHKVRVVSITQPVGDENEPVHAMMRKVIALFDEYQSRENAKHVIRSMKENARQGFWNGATAPLGYKLVEAEKRGTKIKKKLDVDAVEAEYVRLIYKLYLYGDGTSGALGIKEVVKWLNRNGYRTRRGQTFGVAGVHKVLTNTVYIGEWQFNVTSSRTRQRKPDTEIVKIAVPAIIEPHVFEQVQAQLRARSPRVVAPRLTTGPILLTGLAVCATCAGAMTLRTGTSSTGAVHRYYTCSTCARKGKSVCKGRSIPMDKLDGLVTEHLVERLLQPERLAVMLSSLKARRAAKAESENKRIMALQREVSEADERLKRLYRLVEDGVTDLDEVLQGRLSQLKADRDRARAALEATTSRHRADIRIDPALIESFGRTMRENLTTGSTPFRKAYLRTLIDVIEVDDAQIRIKGSKDVLEQAVLASQTGPELRSQMSTKWRTRHDSNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMW3</accession>
    <name>Q3SMW3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Heavy metal efflux pump CzcA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_3131</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316286.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3131"/>
    <dbReference type="EnsemblBacteria" id="ABA06378">
        <property type="protein sequence ID" value="ABA06378"/>
        <property type="gene ID" value="Nwi_3131"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3131"/>
    <dbReference type="eggNOG" id="COG3696">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002755_1_2_5"/>
    <dbReference type="OMA" id="NSWTYPI"/>
    <dbReference type="OrthoDB" id="91817at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2090.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027463">
        <property type="entry name" value="AcrB_DN_DC_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001036">
        <property type="entry name" value="Acrflvin-R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004763">
        <property type="entry name" value="CzcA/CusA/SilA/NccA/HelA/CnrA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32063">
        <property type="entry name" value="PTHR32063"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00873">
        <property type="entry name" value="ACR_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00702">
        <property type="entry name" value="ACRIFLAVINRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82714">
        <property type="entry name" value="SSF82714"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00914">
        <property type="entry name" value="2A0601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="344"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="387"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="428"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="473"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="524"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="864"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="888"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="914"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="975"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1004"/>
            <end position="1030"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA06378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1058" mass="114428" checksum="9C4F863B50742140" modified="2005-10-11" version="1">MIARLIGWSARNLVLVFVGTIFAVAAGLYALKTLPLDAIPDLSDVQVIVYTNYPGQAPQVVEDQVTYPLTTSMLTVPKSKVVRGFSFFGVSFVYVIFEDGTDPYWARSRVLEYLNAAAQRLPAGVTPTLGPDATGVGWVYQYAVVAKEMTLAELRSLQDWVIRFGASKADGVAEVASVGGFVKQYSIVVDPNRLRALGIPLDRVREAVKASNADVGGRTVELSEFEFMVRGRGYIRSAADIENVVLKTDRGVSLRVRDVARVEIEPGERRGITELNGESEVASGIVLQRFGANALTVIENVKARLAEIASSLPEGAEIVPVYDRSELIGRAIETLKGTLIEESIIVALVCVVFLLHLRSALVAILMLPVGIFIAFAAMKAIGLGSNIMSLGGIAIAVGAMIDAAIVMIENAHKHLERAPPDKPRVEILIEAASQVGPALFFSLLVITVSFLPIFTLESQEGRMFGPLAFTKTFAMAAAALLSVTLVPALMVVFVRGRIIPEHRNPINRFLIWVYRPVIRGVLKAKTLTILIALIVLGVTVWPARQLGSEFMPSIDEGALMYMPTTLPGISVTKAAELLQVQDRIIKSFPEVASVHGKAGRALTATDPAPMEMFETIINLKPKSEWRPGVTIDSLKTEMDRALQFPGVSNAWTMPIRARIDMLATGIRTPVGVKVFGTDLAQMEQAAREIERVLRSLPGTSSAYAERVIGGYYLDIVPDRDALGRYGLAIGDVQAVIASALGAETVTTTVEGRERYAVTIRYPRAFRSDPQSIARDVQVPLPGGGTVPLGEVAKVELTRGATSIRTENGQLAVYVFVDIAGRDLGGYVAAAQQAVATEVKLPPGTYVSWSGQFEYLQRAEARLKIVVPVTLLVIFLLLYLNFRALTETLIVMLSLPFALVGGIWLMWWLGFNMSVAVAVGFIALAGVAAETGVIMLIYLEQAMAELKVEREAEGRPFTRTDLYEAIMLGAVERVRPKMMTVIAITAGLIPILWSTGTGSEVMQRIAVPMIGGMVSSTVLTLVVIPAVYALIKGWRLPRSVAADETIIEQCPAGLKAAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMY0</accession>
    <name>Q3SMY0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_3114</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3114"/>
    <dbReference type="EnsemblBacteria" id="ABA06361">
        <property type="protein sequence ID" value="ABA06361"/>
        <property type="gene ID" value="Nwi_3114"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3114"/>
    <dbReference type="HOGENOM" id="CLU_2356888_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="96" mass="10505" checksum="4459BD9DEDF3F824" modified="2005-10-11" version="1">MTELFSSTKPEIAVSTAALAAATGRARYPEGRGLRMTRFPRDSYLDPDRVPDVFCDGVAGVDFIGADVVRLTFYANRSIGGDDDERFIVSVRRQGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMZ5</accession>
    <name>Q3SMZ5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Helix-turn-helix, Fis-type</fullName>
            <ecNumber evidence="3">2.7.7.49</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_3099</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06346">
        <property type="protein sequence ID" value="ABA06346"/>
        <property type="gene ID" value="Nwi_3099"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3099"/>
    <dbReference type="HOGENOM" id="CLU_569646_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010921">
        <property type="entry name" value="Trp_repressor/repl_initiator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48295">
        <property type="entry name" value="SSF48295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="285"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="479" mass="54522" checksum="555084E0680A7F11" modified="2005-10-11" version="1">MCPPSGLLRQFRSFREGRISGSSQPIWSADETEIRTRQSAGRTGAEGHPTADAPALFGGGEDPYRAGRTARRGEHLRALPPRRHRRLDVLRLVQGVPGSRQAPVGGRHGTCRNLWRGERSSSGSLGVEGSRRRSHPGEPSAKKKHERGWGERGMRYPASEKAEIIALVEQSHLPAKRTLDKLGIPRATFYRWYDRYRAGGIEALADHRSRPDRVWNRIPDDVRGQIIDLALELPELSPRELAVRFTDERKYFVSEASVYRLLKAHDLITSPAYVVIKAANEFKDKTTAANQLWQTDFTYLKITGWGWYYLSTVLDDFSRYIVAWRLGPTMCASDVTATLDQALAASGLDHVSVRQRPRLLSDNGSSYVADDLATWLRAKDMQHVRGAPYHPQTQGKIERWHQTLKNRILLENYYLPDDLKRQVAAFVEHYNHDRYHESIGNVTPADVYFGRAETILAERHRIKRDTIANRRLQHQLQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN75</accession>
    <name>Q3SN75_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Thiamine pyrophosphate enzyme</fullName>
            <ecNumber evidence="4">4.1.1.7</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_3016</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.7" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316184.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3016"/>
    <dbReference type="EnsemblBacteria" id="ABA06266">
        <property type="protein sequence ID" value="ABA06266"/>
        <property type="gene ID" value="Nwi_3016"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3016"/>
    <dbReference type="eggNOG" id="COG0028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_8_0_5"/>
    <dbReference type="OMA" id="DFRHEEP"/>
    <dbReference type="OrthoDB" id="391134at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050695">
        <property type="term" value="F:benzoylformate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000399">
        <property type="entry name" value="TPP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="PTHR18968"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00187">
        <property type="entry name" value="TPP_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPP_enzyme_N" evidence="3">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_C" evidence="2">
        <location>
            <begin position="375"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA06266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="514" mass="53903" checksum="DD6F30AC669E0A65" modified="2005-10-11" version="1">MNGAESLVRTLIKSGVDTCFANPGTSEMHFVAALDRVEGMRCVLGLFEGVVTGAADGYFRMKGTPACTLLHLGPGLANGIANLHNARKAGSGIVNIVGQHAIPHIECNAPLTSDIEALARPMSAWIRTSPDSRSVARDGAAAVAAAKSSPPQIATLILPADAAWSEADGVAEVTGASQRPDYSPQAIDGAARILRSGGGTLLLLGGRALTERGLALAAGIAGNTGCQVMAETYNARMARGRGRFFVERIPYPIDQALRALKPFSNIILVEADDPVAFFAYPDKPSRLKPEGCEVHRMTAKDENTIAALEVLAGAVGARPRDAKPQQPMELAWPTGPLNPASIARAIAMAIPENAVVVDESISTGRGFFPPTACAAPHDWLQNMGGSIGFSTPVAIGAAIACPDRKVICMVGDGSAMYTLQSLWTQARESLDVITVVFSNRIYQILRGEFDGVGAGEPGRKAMDMLSLDRPPLDWVALGKGMGVPGRAVTTADEFSMALAEASAEQGPRLIEVQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN86</accession>
    <name>Q3SN86_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_3005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06255">
        <property type="protein sequence ID" value="ABA06255"/>
        <property type="gene ID" value="Nwi_3005"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3005"/>
    <dbReference type="HOGENOM" id="CLU_2480208_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="87" mass="9745" checksum="8775D960940544AC" modified="2005-10-11" version="1">MRIELDAREKKSSGVHARRAQPIASATARRRAADIRLPRKANLGHGPIKMNRIMLKNIIVRSHFLAASRSSRFAGKCSNPSRSRSVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNB1</accession>
    <name>Q3SNB1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aminotransferase</fullName>
            <ecNumber evidence="2">2.6.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2980</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.6.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316152.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2980"/>
    <dbReference type="EnsemblBacteria" id="ABA06230">
        <property type="protein sequence ID" value="ABA06230"/>
        <property type="gene ID" value="Nwi_2980"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2980"/>
    <dbReference type="eggNOG" id="COG0436">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_4_3_5"/>
    <dbReference type="OMA" id="SVAMTGW"/>
    <dbReference type="OrthoDB" id="554560at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="1">
        <location>
            <begin position="32"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="400" mass="43206" checksum="4E99C6F156701938" modified="2005-10-11" version="1">MSFLSAALDRVKPSATIAVTDKARQLKAEGRNVIGLGAGEPDFDTPANIKLAAVHAIEAGKTKYTAVDGIAELKQAIIAKFQRDNELTYQPNQIIVGTGGKQVLYNALMATINPGDEVVIPAPYWVSYPEMVALAGGEPVPVICTAEFGFKLQPEALERAITPKTKWIILNSPSNPTGAAYTRAELKALTDVLVKHPHVWVMTDDMYEHLIYDEFEFTTPAQVEPKLFDRTLTVNGVSKAYCMTGWRIGYAGGPAQLIKAMATIQSQSTSNPSSISQWAAVEALNGPQGFIEANNKVFKQRRDLVVAMLNQASGIDCPRPEGAFYVYPSCAGAIGKTTPSGKTLETDEDFVTELLEAEGVAVVHGSAFGLGPAFRISYATKTADLEEACKRIQRFCGSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNC4</accession>
    <name>Q3SNC4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Peptidase S10, serine carboxypeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2967</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316139.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2967"/>
    <dbReference type="ESTHER" id="nitwn-q3snc4">
        <property type="family name" value="Carboxypeptidase_S10"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06217">
        <property type="protein sequence ID" value="ABA06217"/>
        <property type="gene ID" value="Nwi_2967"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2967"/>
    <dbReference type="eggNOG" id="COG2939">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032786_0_0_5"/>
    <dbReference type="OMA" id="TATAWYH"/>
    <dbReference type="OrthoDB" id="835305at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="PTHR11802"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229045" evidence="2">
        <location>
            <begin position="27"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="517" mass="56292" checksum="D85218EF2C4F2BEF" modified="2005-10-11" version="1">MRRLMIRKACAGAALLAMLAPAAAYAREAAPAAPSHPAETTPAGPRNDGDQPSATADHHRLPPESTTRHKLVLPDRTLAFAATAGAIRVFDDKGEPQADIATTAYQLDGADPRSRPVTFVFNGGPGASSAWLQFGSNGPWRIAMDRAIASPSVPLPLEPNAETWLDFTDLVFIDPLGTGYSRMVARGDDAQKRFFTVDGDVAAIALVIRRWLEKADRMQSPKFIAGESYGGIRGPKIARNLQTRQGVAVKGLILISPALDFSDVLGTGLTRFVASLPSMAATARGKEKGGTLTRADMADAEAYARGEFLTDLVKGQADAAATRRLADRFAALTGIDRAESRRLAGRFDVSEFRRAFDRDGGKVTGRYDASVLGFDPYPDSSASDFNDPSEGPLLTSLTSAAFELHARKLNWRPTGSYELLSRTVNRKWDFGKNRSSVESMSDIRELLAADPQFRLLVAHGLFDLATPYFASQIRLDQLPTFGSRDRIRLVVYPGGHMFYSHDFSRRSLRDEARALMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNN1</accession>
    <name>Q3SNN1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nucleic acid binding, OB-fold, tRNA/helicase-type</fullName>
            <ecNumber evidence="4">2.7.7.7</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2860</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.7" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2860"/>
    <dbReference type="EnsemblBacteria" id="ABA06110">
        <property type="protein sequence ID" value="ABA06110"/>
        <property type="gene ID" value="Nwi_2860"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2860"/>
    <dbReference type="eggNOG" id="COG0587">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065089_0_0_5"/>
    <dbReference type="OMA" id="CEDRFAV"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023073">
        <property type="entry name" value="DNA_pol_error_prone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029460">
        <property type="entry name" value="DNAPol_HHH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004805">
        <property type="entry name" value="PolC_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32294">
        <property type="entry name" value="PTHR32294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32294:SF4">
        <property type="entry name" value="PTHR32294:SF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14579">
        <property type="entry name" value="HHH_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HHH_6" evidence="3">
        <location>
            <begin position="78"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="OB" evidence="2">
        <location>
            <begin position="262"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="344"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="348"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="381"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA06110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="409" mass="44714" checksum="B652EA9375A028EC" modified="2005-10-11" version="1">MIANGYDRDFAERTFSQIEGFGSYGFPESYAASFALLAYASSWLKCHHPDVFCAALLNAQPMGFCAPAQIVRDAKEHGVEVHPVCVNASRWDCTLEMTGCEDRFAVRLGMRMVKGLANADAAAIIATRGEAPFISVDDLSRRASVPAASLVQLADADAFRESLGLPRRDALWAIRALRDTPLPLFAAAARREAATVPEIREPAVALRPMTAGAEVVEDYGHVGLTLRHHPVSFLRGDLARRRIVPCAEAMAARDGRWCEAAGIVLVRQMPGSAKGVMFITIEDETGIANVVVWPKVFEQNRRTILSAGMIGIRGRIQREGDVVHLVAHQLADLSAALASVGERNGEFSVPHGRGDEARHGGSGPDPRELPPRGMRARNFYDPLCRTRHKGSYPESETMPIKLPKPRDFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNQ1</accession>
    <name>Q3SNQ1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phage Gp37Gp68</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2838</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316030.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2838"/>
    <dbReference type="EnsemblBacteria" id="ABA06090">
        <property type="protein sequence ID" value="ABA06090"/>
        <property type="gene ID" value="Nwi_2838"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2838"/>
    <dbReference type="eggNOG" id="COG4422">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054184_0_1_5"/>
    <dbReference type="OMA" id="WHEEWRE"/>
    <dbReference type="OrthoDB" id="979208at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011101">
        <property type="entry name" value="DUF5131"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07505">
        <property type="entry name" value="DUF5131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="255" mass="29290" checksum="C74F361ACF03BBFC" modified="2005-10-11" version="1">MAQKSEIEWTDATWNPVTGCTKVGPGCDNCYAERFAERWRGIVGHPYEQGFDLKLWPSRLEQPFLWKKPRMIFVNSMSDLFHKNIDRTFIDQVFDAMERADWHVYQVLTKRSSLMRNYVRARYSGGPVPRHIWLGVSVEDAAHTSRVEHLKGVNSEARFISFEPLLGRIEDVDLRGIAWAIVGGESGPGARPMQADWARRLRDICDRDDVAFFFKQWGGARPKSGGRLLDGEEWNGFPWQIVPKPILDALQPESV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNV6</accession>
    <name>TRMD_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA (guanine-N(1)-)-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.228</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">M1G-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA [GM37] methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trmD</name>
        <name type="ordered locus">Nwi_2782</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically methylates guanosine-37 in various tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>guanosine(37) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)-methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:36899"/>
            <dbReference type="Rhea" id="RHEA-COMP:10145"/>
            <dbReference type="Rhea" id="RHEA-COMP:10147"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.228"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA methyltransferase TrmD family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.228" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315980.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SNV6"/>
    <dbReference type="SMR" id="Q3SNV6"/>
    <dbReference type="STRING" id="323098.Nwi_2782"/>
    <dbReference type="EnsemblBacteria" id="ABA06035">
        <property type="protein sequence ID" value="ABA06035"/>
        <property type="gene ID" value="Nwi_2782"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2782"/>
    <dbReference type="eggNOG" id="COG0336">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047363_0_1_5"/>
    <dbReference type="OMA" id="ILCGHYK"/>
    <dbReference type="OrthoDB" id="525632at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052906">
        <property type="term" value="F:tRNA (guanine(37)-N(1))-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18080">
        <property type="entry name" value="TrmD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1270.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00605">
        <property type="entry name" value="TrmD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002649">
        <property type="entry name" value="tRNA_m1G_MeTrfase_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023148">
        <property type="entry name" value="tRNA_m1G_MeTrfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016009">
        <property type="entry name" value="tRNA_MeTrfase_TRMD/TRM10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46417">
        <property type="entry name" value="PTHR46417"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01746">
        <property type="entry name" value="tRNA_m1G_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000386">
        <property type="entry name" value="tRNA_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00088">
        <property type="entry name" value="trmD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000257440" description="tRNA (guanine-N(1)-)-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="133"/>
            <end position="138"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="239" mass="25664" checksum="68F895003C4AC3BD" modified="2005-10-11" version="1">MTWRATVLTLFPEMFPGPLGISLAGKALAARLWALDIRDIRNSATDRHRSVDDTPAGGGPGMVLRADVLARAIDAAETPPGAPLLLMSPRGRPLTQSRVAELAAGPGPLIVCGRFEGVDQRIIDARGLDEISIGDYVLSGGEIAAMALIDACVRLLPGVMGKAESGEDESFSHGLLEYPQYTRPQEFEGRVIPDILLSGDHAKVAGWRRAEAEALTQERRPDLWAARATQNPPERKTNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP06</accession>
    <name>Q3SP06_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cell division protein ZapA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2732</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009799004.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2732"/>
    <dbReference type="EnsemblBacteria" id="ABA05985">
        <property type="protein sequence ID" value="ABA05985"/>
        <property type="gene ID" value="Nwi_2732"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2732"/>
    <dbReference type="eggNOG" id="COG3027">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133828_2_0_5"/>
    <dbReference type="OMA" id="FDRYVGH"/>
    <dbReference type="OrthoDB" id="1802106at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007838">
        <property type="entry name" value="Cell_div_ZapA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036192">
        <property type="entry name" value="Cell_div_ZapA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042233">
        <property type="entry name" value="Cell_div_ZapA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34981">
        <property type="entry name" value="PTHR34981"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05164">
        <property type="entry name" value="ZapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102829">
        <property type="entry name" value="SSF102829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="56"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="127" mass="13871" checksum="3F7754D21452A629" modified="2005-10-11" version="1">MSHVNVTINSRQYRMACEDGQEDRLLKLAGDLEGRIGQLRGKFGEIGDQRLEVMAALVTSDELMDAQQRIRALEEELKALRDVRAAAAERARATQTAVVAALNMAAHRIEKTTQVLNRTVGGGIAIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP17</accession>
    <name>Q3SP17_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2721</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315920.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2721"/>
    <dbReference type="EnsemblBacteria" id="ABA05974">
        <property type="protein sequence ID" value="ABA05974"/>
        <property type="gene ID" value="Nwi_2721"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2721"/>
    <dbReference type="eggNOG" id="ENOG5031HJW">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129704_0_0_5"/>
    <dbReference type="OMA" id="FMVFWPL"/>
    <dbReference type="OrthoDB" id="1901641at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021273">
        <property type="entry name" value="DUF2852"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11014">
        <property type="entry name" value="DUF2852"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="162" mass="19434" checksum="A56312E78C8FB8F9" modified="2005-10-11" version="1">MAHTADYDRWNGPNTARGQGQYPSSRFTDAPWHPFRIVLIVLGFMVWWPIGVAILLYTLWSRKMGCWNADRMQYKMDRMRHNMERCGFGFRGFNAPSSGNRAFDEYRMETLRRLEDEQKEFKNFLERLRHAKDKEEFDAFMAQHRTQPTPPNDRPQERPQDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP43</accession>
    <name>Q3SP43_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_2695</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NodD regulates the expression of the nodABCFE genes which encode other nodulation proteins. NodD is also a negative regulator of its own expression. Binds flavonoids as inducers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315894.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2695"/>
    <dbReference type="EnsemblBacteria" id="ABA05948">
        <property type="protein sequence ID" value="ABA05948"/>
        <property type="gene ID" value="Nwi_2695"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2695"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_1_5"/>
    <dbReference type="OMA" id="DLQCEAV"/>
    <dbReference type="OrthoDB" id="750230at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0536">Nodulation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="5">
        <location>
            <begin position="8"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="312" mass="33941" checksum="2913CE1DAAB700BC" modified="2005-10-11" version="1">MITKLRDVTIRQLRALASVAAGGSVTAAANKLHLTQPAVTLQLRNLQQLCGLPLLQRTSEGMTLTPAGQELYTLFERIEAAIDSCATSLDMITGRTGGRISIGAVSTAKYFVPFAISAFSKLHPGMDISLTIVNRQDIRQALRGYALDLAIMGHPPPDIELDTHLIGDHPHVVIAPTGHRLANASGLTAVDLAHEKFLSRKPGSGTRFLMEQFFEKSNLEPQIGMEMSSNETIKQAVIAGLGIAFISAHTVAVELREGRMITLDITGLPIIRQWFVVRKKDKVLLPPAQEMLDFLVANGKEFLPQAPIGRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP67</accession>
    <name>Q3SP67_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pyridoxamine 5'-phosphate oxidase-related, FMN-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2671</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2671"/>
    <dbReference type="EnsemblBacteria" id="ABA05924">
        <property type="protein sequence ID" value="ABA05924"/>
        <property type="gene ID" value="Nwi_2671"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2671"/>
    <dbReference type="eggNOG" id="COG3871">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091428_1_1_5"/>
    <dbReference type="OMA" id="AKAWWDS"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038725">
        <property type="entry name" value="YdaG_split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16242">
        <property type="entry name" value="Pyrid_ox_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyrid_ox_like" evidence="1">
        <location>
            <begin position="23"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="180" mass="20016" checksum="E6B3C1E9FE22C325" modified="2005-10-11" version="1">MMMEHHRIKVRSLKSLAMSRKNDIDRVWDIVEKVGVCMLTTQFAGGLRARPLEARPDRDAGLIFFVTDIRSPKEEEIKARPDIGLAFIGSSDKAYLSITGQACIIRDAEMTRAAWRKTDEVWWPGGPADPSVCLLQIDPSTAELWDGPASSVATAFEFAKAKLTGKKPNLGENRKVTVKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPA0</accession>
    <name>Q3SPA0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2638</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315841.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2638"/>
    <dbReference type="EnsemblBacteria" id="ABA05891">
        <property type="protein sequence ID" value="ABA05891"/>
        <property type="gene ID" value="Nwi_2638"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2638"/>
    <dbReference type="eggNOG" id="ENOG5032ZJ1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_160774_1_0_5"/>
    <dbReference type="OMA" id="NVEEYCI"/>
    <dbReference type="OrthoDB" id="1896101at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021724">
        <property type="entry name" value="DUF3297"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11730">
        <property type="entry name" value="DUF3297"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="95" mass="10712" checksum="1FBAFBAD7B2650A4" modified="2005-10-11" version="1">MNDQTAKDHLPDRLSTDPRSAYYNAELLSRDIGIRFKGVEKSNVEEYCVSEGWIRVAAGNARDRHGNPLTIKLSGTVEPYFRTGDQAWSVIRRSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPD8</accession>
    <name>Q3SPD8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DNA repair photolyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2600</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315803.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2600"/>
    <dbReference type="EnsemblBacteria" id="ABA05853">
        <property type="protein sequence ID" value="ABA05853"/>
        <property type="gene ID" value="Nwi_2600"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2600"/>
    <dbReference type="eggNOG" id="COG1533">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015525_0_0_5"/>
    <dbReference type="OMA" id="GNTDCYQ"/>
    <dbReference type="OrthoDB" id="979208at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040086">
        <property type="entry name" value="MJ0683-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43432">
        <property type="entry name" value="PTHR43432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01084">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="6">
        <location>
            <begin position="91"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="386" mass="43225" checksum="64EA4753E8478858" modified="2005-10-11" version="1">MSRTSPATRRNPVVTSPPASAFASSFAELEVAIDRERRRGRGAQSNVSGRFEAEARTAFDDGWQSLDDLPPFKTTLAVDAARKVITRNESPDISFDRSINPYRGCEHGCVYCFARPTHAYLGLSPGLDFESKLFAKPDAPALLEKELAAEGYEPKMIAIGTNTDPYQPIERGSRIMRGILEVLERASHPVGIVTKSALVTRDIDILARMAKRNLAKVAISVTSLDARLARSMEPRASTPMRRLEALRKLSEAGIPTTVMVAPVIPVLNDSEIERILDAAAHAGVREANYVLLRLPLEVRDLFREWLMANYPDRYRHVFTMIRDMRNGRDYDSQWGARMKGAGPIAWMIGRRFEIACEKLGLNRKRSKLTTDHFARPKRSGQQLSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPG8</accession>
    <name>Q3SPG8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ErfK/YbiS/YcfS/YnhG</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_2570</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YkuD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2570"/>
    <dbReference type="MEROPS" id="C82.003"/>
    <dbReference type="EnsemblBacteria" id="ABA05823">
        <property type="protein sequence ID" value="ABA05823"/>
        <property type="gene ID" value="Nwi_2570"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2570"/>
    <dbReference type="eggNOG" id="COG1376">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042399_1_0_5"/>
    <dbReference type="OMA" id="HMEGGPQ"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071972">
        <property type="term" value="F:peptidoglycan L,D-transpeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16913">
        <property type="entry name" value="YkuD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005490">
        <property type="entry name" value="LD_TPept_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038063">
        <property type="entry name" value="Transpep_catalytic_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03734">
        <property type="entry name" value="YkuD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141523">
        <property type="entry name" value="SSF141523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="YkuD" evidence="5">
        <location>
            <begin position="155"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="101"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="283" mass="31288" checksum="91B875DF8DCB47C5" modified="2005-10-11" version="1">MREGRHDRRGFPATIHGETVQSTSMFRHIPFALLAGVSLADLGIQQASARDLQARFFTEPTVVYAGQSAPQPTGQHLAYAGRRDMGGGFIELLFGDNQRQGSHYQQEPYDSRPLYAPQPQQTMPEQDASEAAHPAFDPKFERQLVEYHGKEAAGTIVIDTSNKFLYLVQGGGKALRYGVGVGKPGFLWAGTKTISAKREWPAWTPPAEMLKRRPDLPRHMVGGPQNPLGARAMYLGSSLYRIHGSNEPWTIGTNVSSGCIRMRNRDVIDLYDRVRVGATVVVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPM0</accession>
    <name>Q3SPM0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two component transcriptional regulator, winged helix family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2518</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315722.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2518"/>
    <dbReference type="EnsemblBacteria" id="ABA05771">
        <property type="protein sequence ID" value="ABA05771"/>
        <property type="gene ID" value="Nwi_2518"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2518"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_4_5"/>
    <dbReference type="OMA" id="KYLMSHP"/>
    <dbReference type="OrthoDB" id="817710at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="26"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="4">
        <location>
            <begin position="150"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="150"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="246" mass="27460" checksum="69E2423DF0F36F42" modified="2005-10-11" version="1">MTVMQMQTQMAGIARARVKLADDAPHLLLVDDDRRIRDLLSRFLTGEGYRVTTAMSAKAARSKLLGLYFDLLILDVMMPGETGFDLARSVRASSPVPIIMLTALHEAENRIEGLQIGADDYLAKPFEPRELVLRIGNILKRTMPMPTSQSESIAFGPYVYHLERGELRQGDDVIHLTDREREMLRVLAATPGETVRRGDLNGGGTVNERAVDVQINRLRRKIERDPANPLFLQAVRGVGYRLVAAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPS7</accession>
    <name>Q3SPS7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PRC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2461</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315669.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2461"/>
    <dbReference type="EnsemblBacteria" id="ABA05714">
        <property type="protein sequence ID" value="ABA05714"/>
        <property type="gene ID" value="Nwi_2461"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2461"/>
    <dbReference type="eggNOG" id="COG1873">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091638_1_0_5"/>
    <dbReference type="OMA" id="PDHAMIN"/>
    <dbReference type="OrthoDB" id="1849143at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="SSF50346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229043" description="PRC domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="PRC" evidence="3">
        <location>
            <begin position="51"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="169" mass="17394" checksum="DEAD3A0F6C6A0694" modified="2005-10-11" version="1">MISKHMTAALIGSALLATAAIAQTPNATMNNNMTAPAAATSSESGYHGSWRSSKMVGLNVYNDTNDKLGSISDLLLDKNGMIKAAVIGVGGFLGVGERLVAVPFDKLKFVDTPAQSASGKGAGTGTTTGSSTSAPATTTTSSNPWFPDHAVFSATKQQLESMPEFKYST</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="130" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPU2</accession>
    <name>Q3SPU2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Carbamoyl-phosphate synthase small chain</fullName>
            <ecNumber evidence="7">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 7">Carbamoyl-phosphate synthetase glutamine chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">carA</name>
        <name evidence="9" type="ordered locus">Nwi_2446</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the CarA family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.5" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315654.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2446"/>
    <dbReference type="EnsemblBacteria" id="ABA05699">
        <property type="protein sequence ID" value="ABA05699"/>
        <property type="gene ID" value="Nwi_2446"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2446"/>
    <dbReference type="eggNOG" id="COG0505">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035901_2_2_5"/>
    <dbReference type="OMA" id="CFNTGMT"/>
    <dbReference type="OrthoDB" id="662268at2"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00171"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01744">
        <property type="entry name" value="GATase1_CPSase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01209">
        <property type="entry name" value="CPSase_S_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006274">
        <property type="entry name" value="CarbamoylP_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002474">
        <property type="entry name" value="CarbamoylP_synth_ssu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036480">
        <property type="entry name" value="CarbP_synth_ssu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035686">
        <property type="entry name" value="CPSase_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00988">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01097">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52021">
        <property type="entry name" value="SSF52021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01368">
        <property type="entry name" value="CPSaseIIsmall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 7" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CPSase_sm_chain" evidence="8">
        <location>
            <begin position="15"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="CPSase" evidence="7">
        <location>
            <begin position="1"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="285"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="369"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="396" mass="42897" checksum="5F63AF7C0FD4032D" modified="2005-10-11" version="1">MTSSENASAWPDLKPTALLVLADGTVIEGFGLGAEGQAVGEVCFNTAMTGYQEILTDPSYAGQLITFTFPHIGNVGTNDEDIETVNMAATPGARGVILRSAITSPSNYRAARHLDAWLRARGIIGISGIDTRALTALIRTKGMPNAVIAHAKDGHFDLYGLKKEAREWPGLEGMDLVPMVTSSQRYTWDETPWEWDKGFGRQDRPEFNVVAVDYGIKRNILRLLAGEGCKITVVPAATSAEDILAMKPDGIFLSNGPGDPAETGKYAVPMIRKVIESGTPTFGICLGHQMLGLAVGAKTKKMHQGHHGANHPVRNETTGKVEITSMNHGFAVDETTLPKGAKQTHVSLFDGSNCGIELEGRPVFSVQYHPEASPGPRDSHYLFRRFADLMRENKRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPU5</accession>
    <name>Q3SPU5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">N-formylglutamate amidohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2443</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315651.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2443"/>
    <dbReference type="EnsemblBacteria" id="ABA05696">
        <property type="protein sequence ID" value="ABA05696"/>
        <property type="gene ID" value="Nwi_2443"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2443"/>
    <dbReference type="eggNOG" id="COG3931">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079628_0_0_5"/>
    <dbReference type="OMA" id="IDCNRPP"/>
    <dbReference type="OrthoDB" id="1449483at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007709">
        <property type="entry name" value="N-FG_amidohydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011227">
        <property type="entry name" value="UCP029730"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05013">
        <property type="entry name" value="FGase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029730">
        <property type="entry name" value="UCP029730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="266" mass="29140" checksum="5F739EC78914D77A" modified="2005-10-11" version="1">MTAPDAQPAIIALHEPPVIERNVSGLSPFLFTCDHYGRLIPDALGDLGLPESERRRHIAWDIGIAGVAERLSAALGAHLIAQRYSRLVIDCNRPPHAASSIPALSEATVIPGNERLSQSEADARRHAIFEPYHRRIKERIDERLARSRPTILVSLHSFTPVYAGVARPWHVGTLYHRSTELPPLLLSLLRAEGDLVVGDNEPYAVSDLTDYTIPVHGEARGLMNSGIEIRQDLIGDDAGQVAWADRLARALREVEATLIASQSSCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPU6</accession>
    <name>Q3SPU6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2442</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="J. Bacteriol." volume="193" first="2408" last="2417">
            <title>Unexpected diversity of chlorite dismutases: a catalytically efficient dimeric enzyme from Nitrobacter winogradskyi.</title>
            <authorList>
                <person name="Mlynek G."/>
                <person name="Sjoblom B."/>
                <person name="Kostan J."/>
                <person name="Fureder S."/>
                <person name="Maixner F."/>
                <person name="Gysel K."/>
                <person name="Furtmuller P.G."/>
                <person name="Obinger C."/>
                <person name="Wagner M."/>
                <person name="Daims H."/>
                <person name="Djinovic-Carugo K."/>
            </authorList>
            <dbReference type="PubMed" id="21441524"/>
            <dbReference type="DOI" id="10.1128/JB.01262-10"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) IN COMPLEX WITH HEME</scope>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315650.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="PDB" id="3QPI">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.10 A"/>
        <property type="chains" value="A/B=1-183"/>
    </dbReference>
    <dbReference type="PDBsum" id="3QPI"/>
    <dbReference type="SMR" id="Q3SPU6"/>
    <dbReference type="STRING" id="323098.Nwi_2442"/>
    <dbReference type="EnsemblBacteria" id="ABA05695">
        <property type="protein sequence ID" value="ABA05695"/>
        <property type="gene ID" value="Nwi_2442"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2442"/>
    <dbReference type="eggNOG" id="COG3253">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095674_0_0_5"/>
    <dbReference type="OMA" id="FDFLTWF"/>
    <dbReference type="OrthoDB" id="1451977at2"/>
    <dbReference type="BRENDA" id="1.13.11.49">
        <property type="organism ID" value="3651"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010644">
        <property type="entry name" value="ChdC/CLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06778">
        <property type="entry name" value="Chlor_dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="6" id="KW-0002">3D-structure</keyword>
    <keyword evidence="1 6" id="KW-0349">Heme</keyword>
    <keyword evidence="3 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Heme</name>
            <dbReference type="ChEBI" id="CHEBI:17627"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
            <note>axial binding residue</note>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="3QPI"/>
        </source>
    </evidence>
    <sequence length="183" mass="20391" checksum="2A49F55950A2B203" modified="2005-10-11" version="1">MTFTVFTGGDSGAWSILSVAPVIGESLMAASHLAIAPSLSLGDTSATTPWQLRGVASHARYVERAEKIALTSVQAGLGRNEATRAALIPIRKSAAWWEMTQDERRAIFEDKSHHIAASLKYLPAIARQLYHCRDIGEPFDFLTWFEYAPEHATMFEDLVGVLRATEEWTYVEREVDIRLARAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ09</accession>
    <name>Q3SQ09_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Methionine adenosyltransferase</fullName>
            <ecNumber evidence="6">2.5.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">Nwi_2378</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-methionine = diphosphate + phosphate + S-adenosyl-L-methionine</text>
            <dbReference type="Rhea" id="RHEA:21080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 15">Belongs to the AdoMet synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.6" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315593.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2378"/>
    <dbReference type="EnsemblBacteria" id="ABA05632">
        <property type="protein sequence ID" value="ABA05632"/>
        <property type="gene ID" value="Nwi_2378"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2378"/>
    <dbReference type="eggNOG" id="COG0192">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041802_1_1_5"/>
    <dbReference type="OMA" id="MPYLRPD"/>
    <dbReference type="OrthoDB" id="1024388at2"/>
    <dbReference type="UniPathway" id="UPA00315">
        <property type="reaction ID" value="UER00080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004478">
        <property type="term" value="F:methionine adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022631">
        <property type="entry name" value="ADOMET_SYNTHASE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022630">
        <property type="entry name" value="S-AdoMet_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022629">
        <property type="entry name" value="S-AdoMet_synt_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022628">
        <property type="entry name" value="S-AdoMet_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002133">
        <property type="entry name" value="S-AdoMet_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022636">
        <property type="entry name" value="S-AdoMet_synthetase_sfam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11964">
        <property type="entry name" value="PTHR11964"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02773">
        <property type="entry name" value="S-AdoMet_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02772">
        <property type="entry name" value="S-AdoMet_synt_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00438">
        <property type="entry name" value="S-AdoMet_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000497">
        <property type="entry name" value="MAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55973">
        <property type="entry name" value="SSF55973"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01034">
        <property type="entry name" value="metK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00376">
        <property type="entry name" value="ADOMET_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00377">
        <property type="entry name" value="ADOMET_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="13 14" id="KW-0630">Potassium</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 19" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S-AdoMet_synt_N" evidence="16">
        <location>
            <begin position="4"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="S-AdoMet_synt_M" evidence="17">
        <location>
            <begin position="126"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="S-AdoMet_synt_C" evidence="18">
        <location>
            <begin position="245"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ABA05632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="398" mass="43272" checksum="63E2481CAF45894F" modified="2005-10-11" version="1">MRGSYLFTSESVSEGHPDKVCDRISDEVVDLFFSEGPKAGIDPWAIRAACETLATTNKVVIAGETRGPESVTRDMIESVVRGAIKDIGYEQDGFHWKTADIEILLHPQSADIAQGVDALQPGANKEEGAGDQGIMFGYACNETPDLMPAPIFYAHKILRLISEARHSGKEKVLGPDSKSQVTVQYENGKPVGVREIVVSHQHLVEDMSSDQVRERVEPYVREALPKGWITDKTIWHINPTGKFFIGGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYAARYLAKNIVAAGLADRCTLQLAYAIGVARPLSIYIDTHGTGKAPEDTLERVVSEAMDLTPRGIRKHLDLNRPIYARTSSYGHFGRTPDNEGGFSWEKTDLAEVIKRAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ40</accession>
    <name>Q3SQ40_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2347</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315565.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2347"/>
    <dbReference type="EnsemblBacteria" id="ABA05601">
        <property type="protein sequence ID" value="ABA05601"/>
        <property type="gene ID" value="Nwi_2347"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2347"/>
    <dbReference type="eggNOG" id="COG3242">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_179416_2_1_5"/>
    <dbReference type="OMA" id="ILIWAVR"/>
    <dbReference type="OrthoDB" id="2079765at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019201">
        <property type="entry name" value="DUF2065"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38602">
        <property type="entry name" value="PTHR38602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09838">
        <property type="entry name" value="DUF2065"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="66" mass="7068" checksum="61156B35F1D42859" modified="2005-10-11" version="1">MGSIAFADFLIGLGILFMLEGILFAAAPKWVRRAMESALAAPEQVLRIAGIGSAIAGLILIWLVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ56</accession>
    <name>Q3SQ56_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Guanosine polyphosphate pyrophosphohydrolase/synthetase</fullName>
            <ecNumber evidence="1">3.1.7.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2331</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.7.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2331"/>
    <dbReference type="EnsemblBacteria" id="ABA05585">
        <property type="protein sequence ID" value="ABA05585"/>
        <property type="gene ID" value="Nwi_2331"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2331"/>
    <dbReference type="eggNOG" id="COG0317">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1641963_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008893">
        <property type="term" value="F:guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="161" mass="18090" checksum="240218209943D882" modified="2005-10-11" version="1">MMALSKLYDEALVYASDLHRTQVRKGSSTPYIAHLLSVSSRVLSAGGTEVQAIAGLLHDTAEDQGGQATLDEVRKRFGSDVAQIVADCTDSWVEPTPAWRPRKEVYLSLLPKKPTSSLLVPCRQGRQCRSDTERLSQHRRRIVAPLYRWARRDHLVLPRAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ93</accession>
    <name>Q3SQ93_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">TonB-dependent siderophore receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_2294</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the TonB-dependent receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315515.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2294"/>
    <dbReference type="EnsemblBacteria" id="ABA05548">
        <property type="protein sequence ID" value="ABA05548"/>
        <property type="gene ID" value="Nwi_2294"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2294"/>
    <dbReference type="eggNOG" id="COG4774">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008287_22_0_5"/>
    <dbReference type="OMA" id="AMDVPFN"/>
    <dbReference type="OrthoDB" id="196825at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015344">
        <property type="term" value="F:siderophore uptake transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030149">
        <property type="entry name" value="FcuA/FatA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012910">
        <property type="entry name" value="Plug_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037066">
        <property type="entry name" value="Plug_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039423">
        <property type="entry name" value="TonB-dep_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000531">
        <property type="entry name" value="TonB-dep_rcpt_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036942">
        <property type="entry name" value="TonB_rcpt_b-brl_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010105">
        <property type="entry name" value="TonB_sidphr_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32552">
        <property type="entry name" value="PTHR32552"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32552:SF82">
        <property type="entry name" value="PTHR32552:SF82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07715">
        <property type="entry name" value="Plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00593">
        <property type="entry name" value="TonB_dep_Rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01783">
        <property type="entry name" value="TonB-siderophor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="3 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0798">TonB box</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229127" evidence="8">
        <location>
            <begin position="27"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="domain" description="Plug" evidence="10">
        <location>
            <begin position="120"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="TonB_dep_Rec" evidence="9">
        <location>
            <begin position="291"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="26"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="75"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA05548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="773" mass="83308" checksum="54EA002F33FCC395" modified="2005-10-11" version="1">MRRQVGQIALIISGLGVLPLSESAIAQSSSSNGGTTLTPIEVIAPRTRPATRNRTVRATPNPHRAAVRPRSREAAPPPAAAPSPPALPQTGTVGQPPAPYAGGQVGTGVRMGMLGNTSVLTTPFNVTGYTSKLMEDQRARSIADVTLNDPSVRNDAPPFSERDSFFIRGFSVTNLDTAYDGLFYLANPRRSFLEGIERVEVLKGPSALLSGGTARTGGTINMIPKRAGDEPLTRLTTTYISNSQIWNHADLGRRFGENKEWGVRFNGSYRKGDTPLDLNSAEVGVAALGLDYRGERFRASLDFNSSVQNIRAPTSLFNSAAPNIIIPHAQNGRINTSSRDEFIDSRYKMVAGRAEYDLLPDTTVYIAGGGSRYNEDFLTSSYRITNPNGTATNSLAIQPQKLQGFAGEMGLRSKFRTGVIGHQFNVAAVTMTNELYRGGVLGFTPFSYVTNIYNPVRLPPGSFQTTGFARSDDRPLFTRLVARSVAVSDTLSVFDDRLLLTLGGRWQDIAITGYATAPGPNLGMETSRNQQARFSPAVGAVLRATDRLSLYTNYIESLDAGPTAPALAGNSGTVFPPVVSRQQEVGAKYDFGTVTVTASLFEIRQPFAFTDPGTNLFSVAGLQRNRGFELNVFGSPIEGLRLLGGIALMDARQVNTIGGRFDGNNVPGVPVTALNLYGEYDLPHWMAPGVTMTGRLIRTGDVFYDQANTQSVSGWTRVDLGARYTFVGPLGKPIVMRAIVENVADNAYYLSAARGFLAVGAPRTYMLSATFNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQA4</accession>
    <name>Q3SQA4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acriflavin resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2283</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315504.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2283"/>
    <dbReference type="EnsemblBacteria" id="ABA05537">
        <property type="protein sequence ID" value="ABA05537"/>
        <property type="gene ID" value="Nwi_2283"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2283"/>
    <dbReference type="eggNOG" id="COG0841">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002755_1_2_5"/>
    <dbReference type="OMA" id="REYPKMT"/>
    <dbReference type="OrthoDB" id="145346at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2090.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027463">
        <property type="entry name" value="AcrB_DN_DC_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001036">
        <property type="entry name" value="Acrflvin-R"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32063">
        <property type="entry name" value="PTHR32063"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00873">
        <property type="entry name" value="ACR_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00702">
        <property type="entry name" value="ACRIFLAVINRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82714">
        <property type="entry name" value="SSF82714"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="337"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="362"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="430"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="462"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="524"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="846"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="872"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="943"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="976"/>
            <end position="1002"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1031" mass="110985" checksum="BFC810E29F380009" modified="2005-10-11" version="1">MAFTDIFIKRPVLSIVVSLLILLIGLAAAFNLPVRQYPKLSNTVITVTTIYPGASPDLMQGFITTPIEQAVASAEGVDYITSTSTQGQSQIKVYVKLNFDPNAALTEVLSKVNSVRYLIPKEANDPVILKSTGETTAVMYIGFSSDELSGSAISDYLTRVVQPILSTVDGVASADILGGQTFAMRVWLDPMKMAGRNVTTAEIAIAIAANNFQAAAGQTKGYYTISNVTTNTDLQSVEQFKRMTVKAKDGGFVRMEDIATVELAAQSTNASVAFSGQRAIFIGVQATPQGNPLNIVAGVRALFPDIERNLPPSMKMKVAYDSTKFITSSIHEVQNTLIEAVLIVIVVIFLFLASLRSVIIPVVTIPLSLIGVCALMLAAGFSFNLLTLLAMVLAIGLVVDDAIVVVENIHRHLEEGKSAVQASLQGAREIVGPVISMTITLAAVYAPIGFLGGLTGALFREFAFTLAGAVIVSGVIALTLSPMMCSVLLKHGGQGWFATKVDRIFSSVTDWYGRKLDRSLDYRPVTALFAVTLLGLVGFLYMNTSTELAPEEDQGIVFAVIKAPKYANLDYMDFYGNKLDKAYASFPETDLRFVINGTNGINDGIAGMLLKPWDERTRSAMTIKPLVQAEISKVEGVSAFAFNRPPLPGGPGGLPVQMVLYSTSSFQTVYEEMNKLKEAARKSGLFIVSDSDLDYNQPVVRVAIDRTKANDLGVTMQQLGGTLATLLGGNYVNRFNLQGRSYQVIPQVPRGERLSPQSLDGYYVPGAGGQQIPLSTLVSIKTETDPNALTHYNQLNAATFQAVPMPGVTVGKAVEFLEQQAKNLPKGFSHDYLADARQYVTEGNQLAIAFGFALIIIFLVLAAQFESLRDPLVIMISVPMAILGALIPLFFGVATINIYTQVGLLTLIGLISKHGILMVEFANELQINEKLDKRAAIEKAARVRLRPILMTTAAMVTGLLPLLTASGAGAASRFSIGLVVVAGMMIGTLFTLFVLPAVYVAIATDHLSGTKSERRRQIAEFDMESKALRPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQH2</accession>
    <name>Q3SQH2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Peptidase S58, DmpA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2215</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S58 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315438.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2215"/>
    <dbReference type="MEROPS" id="P01.101"/>
    <dbReference type="EnsemblBacteria" id="ABA05469">
        <property type="protein sequence ID" value="ABA05469"/>
        <property type="gene ID" value="Nwi_2215"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2215"/>
    <dbReference type="eggNOG" id="COG3191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044458_1_0_5"/>
    <dbReference type="OMA" id="PHFWAAP"/>
    <dbReference type="OrthoDB" id="2000083at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016117">
        <property type="entry name" value="ArgJ-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005321">
        <property type="entry name" value="Peptidase_S58_DmpA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36512">
        <property type="entry name" value="PTHR36512"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03576">
        <property type="entry name" value="Peptidase_S58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56266">
        <property type="entry name" value="SSF56266"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="332" mass="33762" checksum="D3CC299705BED4FD" modified="2005-10-11" version="1">MKNLLTDISGVRVGHADDARLASGVTAVLFDTPVVAAMDVRGGGPGTRDSTLLDPVNTVERIDALALSGGSAFGVESGGGVQAWLAEQGRGFVIGDATVPIVPGAVVFDLLNGGDKKWGRFAPYRDLGYAAAAAAGEDFALGSTGAGLGATTANLKGGIGSASAATPGGVKVAALAVVNAVGSVTVGDGPWFWAVPFETGDEYGGRGLPPSFTPDMLAMRLKDGATAKAGENTTLAIVVTDAVLTKPQARRLAIMSQTGFARAIYPVHAPLDGDIVFAAATGDKPIDPFVELTELGMIAANTVARAIARGVYEAKALPFPDALPAWKDRFAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQN4</accession>
    <name>Q3SQN4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATP-binding protein Uup</fullName>
            <ecNumber evidence="6">3.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">uup</name>
        <name evidence="10" type="ordered locus">Nwi_2153</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in beta-(1--&gt;2)glucan export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <comment type="function">
        <text evidence="6">Probably plays a role in ribosome assembly or function. May be involved in resolution of branched DNA intermediates that result from template switching in postreplication gaps. Binds DNA and has ATPase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="6">Associates with ribosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ABC transporter superfamily. ABCF family. Uup subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315376.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2153"/>
    <dbReference type="EnsemblBacteria" id="ABA05407">
        <property type="protein sequence ID" value="ABA05407"/>
        <property type="gene ID" value="Nwi_2153"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2153"/>
    <dbReference type="eggNOG" id="COG0488">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_36_0_5"/>
    <dbReference type="OMA" id="AQADKMR"/>
    <dbReference type="OrthoDB" id="1112046at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00848">
        <property type="entry name" value="Uup"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032524">
        <property type="entry name" value="ABC_tran_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043686">
        <property type="entry name" value="Uup"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037118">
        <property type="entry name" value="Val-tRNA_synth_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16326">
        <property type="entry name" value="ABC_tran_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="6"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="283"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="502"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="534"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <begin position="315"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA05407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="602" mass="66265" checksum="6DB2A910F1F63B5F" modified="2005-10-11" version="1">MAPPLVQLKDIALTFGGTPLLSGVELTVSESQRLCLIGRNGSGKSTLLKIAAGLVAPDSGSRFVQPGATVRYLPQEPDFAGFETTLTYVEAGLGPGDDPYQARYLVEQLGLTGEEDPAHLSGGESRRAALARVLAPSPDILLLDEPTNHLDLTTIEWLEGELGARRGALVLISHDRRFLSNLSRSTAWLDRGRIRQIDRGFAKFEEWRDEVLAEEERDQHKLDRKIVAEEHWLRYGVSGRRKRNVKRLAGLHELRQQRRDYRGTAGSATLAAAEADKSGKLVIEAKGISKRYGERTIVDGFSIRIQRGDRIGIVGPNGAGKTTLINMLTGADTPDSGAIRLGANIEMATLDQHRESLDPKSTLTEALTGGRGDHVMVGGKPKHVVSYMKDFLFAQEQMRTPLEVLSGGERGRLMLARALAKPSNLLVLDEPTNDLDLETLDVLEDMLGDYEGTVILISHDRDFLDRVVTSVIVPEGNGRWIEYAGGYTDMLAQRGADLKREATKAAPVPEAGRSAKPAPAAGKRRLSFNEKHALETLPKSIEKLQAEMARQQRRLDDPDLYARDRAAFDQASAALAKAQAELQQAEDRWLELEMLREEIEQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQN8</accession>
    <name>Q3SQN8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransf_25 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2149</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315372.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2149"/>
    <dbReference type="EnsemblBacteria" id="ABA05403">
        <property type="protein sequence ID" value="ABA05403"/>
        <property type="gene ID" value="Nwi_2149"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2149"/>
    <dbReference type="eggNOG" id="COG0500">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081790_0_0_5"/>
    <dbReference type="OMA" id="FRGWVAY"/>
    <dbReference type="OrthoDB" id="1739932at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransf_25" evidence="1">
        <location>
            <begin position="44"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="223" mass="24937" checksum="165CDC77CF13BB5C" modified="2005-10-11" version="1">MEPFSGAVVNTYAEGPPRQVPGFADLHRMTTMLLVERVPDDGRVLVLGAGGGLELKAFAESHPGWSFDAVDPSAPMLRLAERTIERHAKRVHFHESYIDDAPQGPFDAATSLLTFHFIPREKRPGTLKQIRRRLKPGAPFVVAHISFPQTEPERSVWIARHVAFGARHDTSPDQMEKSRQAIADRLSILAPEEEERMLQEAGFSNVSLFYAGFSFKGWLSYAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQQ7</accession>
    <name>Q3SQQ7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Toprim domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2129</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315355.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2129"/>
    <dbReference type="EnsemblBacteria" id="ABA05384">
        <property type="protein sequence ID" value="ABA05384"/>
        <property type="gene ID" value="Nwi_2129"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2129"/>
    <dbReference type="eggNOG" id="COG4643">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_803799_0_0_5"/>
    <dbReference type="OMA" id="GHREGRY"/>
    <dbReference type="OrthoDB" id="1071997at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13362">
        <property type="entry name" value="Toprim_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Toprim" evidence="2">
        <location>
            <begin position="240"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="353" mass="38296" checksum="1CA521C9A0D9BEA2" modified="2005-10-11" version="1">MSRLDASELARRLARNAEAVCRHYLSNGHREGRYWMVGDVRNTPGRSMFVRLIGPESGKGAAGKWTDAATGEHGDLLDVIRETCGLVDFADVAREARAFLSLPQPEPQASERRRTIQPAPTGSPEAARRLFAMSRPIHGTLAETYLRRRGITALHGTGSLRFHPRCYYRSDEYAATETWPAMIAAVTDLGGRLTGAHRTWLDPDGFDPVRLGKAPIDTPRRAMGDLLGHAVRFGVAGEVMAAGEGIETMLSLRTVLPGMPMVAALSAAHLSAILFADTLRRLYIARDDDPAGDGAMAALIERACADSIEAIVLSPRLADFNEDLRMLGPDALRAALRAQIASRDAARFMAEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQY2</accession>
    <name>Q3SQY2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fec I like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2051</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315290.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2051"/>
    <dbReference type="EnsemblBacteria" id="ABA05309">
        <property type="protein sequence ID" value="ABA05309"/>
        <property type="gene ID" value="Nwi_2051"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2051"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_12_5_5"/>
    <dbReference type="OMA" id="CAGNIAI"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="2">
        <location>
            <begin position="18"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="3">
        <location>
            <begin position="117"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="176" mass="19671" checksum="42519C7FB5687EE5" modified="2005-10-11" version="1">MDTAATTQSDDDLMSAYLRHWKALCAHLRKRTGSQELAEDALQETWLRLAGMKGDAAAIQDHHAFILRVAGNIAIDLVRRERRHAVHCVSDETLLAAVADNMPSPEVCVIDRDQLRLLALALARLPAKPRAALLLSRCDGLSHREIGARLKVSESMVAKYLGQALRHCRDHYRHQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQZ0</accession>
    <name>Q3SQZ0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Secretion protein HlyD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2042</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315284.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2042"/>
    <dbReference type="EnsemblBacteria" id="ABA05301">
        <property type="protein sequence ID" value="ABA05301"/>
        <property type="gene ID" value="Nwi_2042"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2042"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018816_14_1_5"/>
    <dbReference type="OMA" id="YANIYSP"/>
    <dbReference type="OrthoDB" id="1532186at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032317">
        <property type="entry name" value="HlyD_D23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006143">
        <property type="entry name" value="RND_pump_MFP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16576">
        <property type="entry name" value="HlyD_D23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01730">
        <property type="entry name" value="RND_mfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="HlyD_D23" evidence="4">
        <location>
            <begin position="75"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="122"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="414" mass="44386" checksum="AEF1AE12DBD31213" modified="2005-10-11" version="1">MPTMNAPVPPDDVDGLALPPAWTAPARRARGSRRLVLGLSAAGLAALGTFAVMGFRAPSPDSLYETVAVTRQDIESTVAATGKIQPYASVDVGAQVTGQLKHLRVAVGDKVTAGRVVAEIDAEVQSAKVEGIEADLARLRAELAEQEAGLVFAERNVERHQTLSQTSAVSKLAFETTTRDRDILIARIDASKARIRQMQADLRAQKTALRFARITAPMGGTVVSVDAKEGQTLNANYDTPLILQIADLDTMTVWTDVSEADVVKLREGMPLWFTTLGRPDRKWHATLRQIQPAPRRPDNKKAETKTTPQSGNVVLYTALFDVPNADGSLRAGMTAQVFFVTAAAQNALVVPVASLNDKGEVRVLREDGTIAVRKVEIGTRTRFLVGITSGVADGERVITGLKSAGPPAKLRVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR75</accession>
    <name>Q3SR75_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1956</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315207.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1956"/>
    <dbReference type="EnsemblBacteria" id="ABA05216">
        <property type="protein sequence ID" value="ABA05216"/>
        <property type="gene ID" value="Nwi_1956"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1956"/>
    <dbReference type="eggNOG" id="COG2405">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1553682_0_0_5"/>
    <dbReference type="OrthoDB" id="1700444at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="172" mass="18658" checksum="FC19BDCB9048AEA0" modified="2005-10-11" version="1">MGTGIVIADTSVLVNFLRVDRMDLIGAHPNPFIVTDHVAEEITSSYPQQQACFQAALTVGYVAQHRIDDPAELDMFARLSAKGRLGAGERSAISVALNRGYSLAMDDSRAIRRAVQEAGMIGTALTIIRTQDLMVQLIQAGLITVGAADVILNDWSKNHRFKLKIGSFSEVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRA2</accession>
    <name>Q3SRA2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">4-hydroxy-tetrahydrodipicolinate synthase</fullName>
            <shortName evidence="9">HTPA synthase</shortName>
            <ecNumber evidence="4 9">4.3.3.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">dapA</name>
        <name evidence="11" type="ordered locus">Nwi_1929</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 9">Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>L-aspartate 4-semialdehyde + pyruvate = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:34171"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="4.3.3.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the DapA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.7" evidence="4 9"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315185.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1929"/>
    <dbReference type="EnsemblBacteria" id="ABA05189">
        <property type="protein sequence ID" value="ABA05189"/>
        <property type="gene ID" value="Nwi_1929"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1929"/>
    <dbReference type="eggNOG" id="COG0329">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049343_7_1_5"/>
    <dbReference type="OMA" id="GMDACVP"/>
    <dbReference type="OrthoDB" id="1438588at2"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00017"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008840">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00950">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00418">
        <property type="entry name" value="DapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005263">
        <property type="entry name" value="DapA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002220">
        <property type="entry name" value="DapA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020625">
        <property type="entry name" value="Schiff_base-form_aldolases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020624">
        <property type="entry name" value="Schiff_base-form_aldolases_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128">
        <property type="entry name" value="PTHR12128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00701">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001365">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00146">
        <property type="entry name" value="DHPICSNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01130">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00674">
        <property type="entry name" value="dapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00665">
        <property type="entry name" value="DHDPS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00666">
        <property type="entry name" value="DHDPS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="9 10 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 9" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0704">Schiff base</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="9">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="site" description="Part of a proton relay during catalysis" evidence="9">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <feature type="site" description="Part of a proton relay during catalysis" evidence="9">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR001365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA05189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="296" mass="31437" checksum="BE5E4F1D838DCA37" modified="2005-10-11" version="1">MVSRAKFQGSYTALVTPFKNGSVDEAAFRSLVSWQIGEGVHGLVPVGTTGESPTLSHDEHRKVVEWCIEEAGGRVPVIAGAGSNSTREAVDLALHAEKAGADAVLVVTPYYNKPTQEGLYQHFKTVNDAVGIPIIIYNIPSRSVVDLSVETMARLFELKNIAGVKDATGNLARVSLQRRAMGPDFIQLSGEDMTALAFMAAGGRGCISVVANVAPKLCASLMSAVLKGDYAAGLEIQDRLVPLHDAIFKEPGLAGAKHALKLLGRIDEVVRLPLIPVTPPTGKLIREAMVHAGLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRA9</accession>
    <name>Q3SRA9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GTP pyrophosphokinase rsh</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">(p)ppGpp synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">ATP:GTP 3'-pyrophosphotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_1922</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">In eubacteria ppGpp (guanosine 3'-diphosphate 5'-diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the relA/spoT family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1922"/>
    <dbReference type="EnsemblBacteria" id="ABA05182">
        <property type="protein sequence ID" value="ABA05182"/>
        <property type="gene ID" value="Nwi_1922"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1922"/>
    <dbReference type="eggNOG" id="COG0317">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012300_3_0_5"/>
    <dbReference type="OMA" id="DWISSPK"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015969">
        <property type="term" value="P:guanosine tetraphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05399">
        <property type="entry name" value="NT_Rel-Spo_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01668">
        <property type="entry name" value="TGS_RSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004811">
        <property type="entry name" value="RelA/Spo_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045600">
        <property type="entry name" value="RelA/SpoT_AH_RIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007685">
        <property type="entry name" value="RelA_SpoT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033655">
        <property type="entry name" value="TGS_RelA/SpoT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13291">
        <property type="entry name" value="ACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19296">
        <property type="entry name" value="DUF5913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04607">
        <property type="entry name" value="RelA_SpoT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00954">
        <property type="entry name" value="RelA_SpoT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="SSF81271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00691">
        <property type="entry name" value="spoT_relA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HD" evidence="7">
        <location>
            <begin position="92"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="8">
        <location>
            <begin position="436"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="6">
        <location>
            <begin position="700"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="12"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00014315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00032407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="774" mass="85683" checksum="D06EC109F0245136" modified="2005-10-11" version="1">MKGGFRAMKTLGSMTGWPGKRRQMEAATDPVVTAPPPAAPVKPTRPRMMRQYDLVERVRSYNPDTNEDMLNRAYVYAMKAHGEQIRASGDPYFSHPLEVAAILTGLKLDDATIVAALLHDTIEDTEATRAEIDNMFGPEIGALVEGLTKLKRLELVSREAKQAENLRKLLLAIADDVRVLLVKLADRLHNMRTLEFVPPASRQRIAEETLDIYAPLAGRMGMQEMREELEDLAFQTLDPEAHGVVAQRLDALAERNRNLIGEIESQLTTSLANKGIAARVAGRRKQPFSIWTKMERKSVGFEQLSDIFGFRVVVPDVEACYRALGVVHMTWPVVPGRFKDYISTPKQNDYRSIHTTVIGPGNQRVELQLRTEEMDRIAEHGIAAHAFYKDGAGSPTELLNRESNAFAWLRHTIEVLSESANPEEFLEHTKLELFHDQVFCFTPKGKLIALPRHANVIDFAYAVHTDVGNSTVGCKINGKFAPLSSRLQNGDEVEVLTSAAQSAPPSAWESLAVTGKARAAIRRATRAAVRGQYVSLGRRIVERLFIRAKIEYADDKLTGALPRLARATIEDVMASVGRGEMRAADVARAMYPDYKEERVGGPGVKKSLTAKLKLKTPLDPARSPSVIPIGGINSDLPIKFAPNGGAVPGDRIVGIVTPGEGITIYPIQSPALTDFEEEPERWLDVRWDVDDTTPQRFPARIVLDSLNEPGSLAQVATVIAEHDGNIDNISMTRRSPDFTEMTIDLEVYDLKHLSAIIAQLRAKDVVARIERVNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRD9</accession>
    <name>Q3SRD9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Histone-like DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1892</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial histone-like protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315148.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1892"/>
    <dbReference type="EnsemblBacteria" id="ABA05152">
        <property type="protein sequence ID" value="ABA05152"/>
        <property type="gene ID" value="Nwi_1892"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1892"/>
    <dbReference type="eggNOG" id="COG0776">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105066_3_0_5"/>
    <dbReference type="OMA" id="PAFKISK"/>
    <dbReference type="OrthoDB" id="1847472at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000119">
        <property type="entry name" value="Hist_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010992">
        <property type="entry name" value="IHF-like_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33175">
        <property type="entry name" value="PTHR33175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00216">
        <property type="entry name" value="Bac_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01727">
        <property type="entry name" value="DNABINDINGHU"/>
    </dbReference>
    <dbReference type="SMART" id="SM00411">
        <property type="entry name" value="BHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47729">
        <property type="entry name" value="SSF47729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="99" mass="10764" checksum="BAF4FAF455DA6FED" modified="2005-10-11" version="1">MAKKAATPSTVTLKHLAAALAEDHELSKKAAEAILSDMVTRITKHLKKGDRIRIVGLGILQVRKRAARKGRNPATGEEIQIKASKKVAFRAAKELKEAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRL1</accession>
    <name>Q3SRL1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Septum formation initiator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1819</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315076.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1819"/>
    <dbReference type="EnsemblBacteria" id="ABA05080">
        <property type="protein sequence ID" value="ABA05080"/>
        <property type="gene ID" value="Nwi_1819"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1819"/>
    <dbReference type="eggNOG" id="COG2919">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159931_0_0_5"/>
    <dbReference type="OMA" id="RYQLNMS"/>
    <dbReference type="OrthoDB" id="1982186at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007060">
        <property type="entry name" value="FtsL/DivIC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04977">
        <property type="entry name" value="DivIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="41"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="105" mass="11888" checksum="286A8E1BCEBC4EB5" modified="2005-10-11" version="1">MVSRSRLRSLLTGLALYAMAAMIVGYFGVNAYTGKYGLNARQDLDQEIIALTSELARLKAERAEGEKRVALLRASGIDPDMLDERVRYQLHYANPHELIHVLRRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRN2</accession>
    <name>Q3SRN2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="2">PPIase</shortName>
            <ecNumber evidence="2">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1798</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315055.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1798"/>
    <dbReference type="EnsemblBacteria" id="ABA05059">
        <property type="protein sequence ID" value="ABA05059"/>
        <property type="gene ID" value="Nwi_1798"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1798"/>
    <dbReference type="eggNOG" id="COG0652">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_16_6_5"/>
    <dbReference type="OMA" id="VPFHRVM"/>
    <dbReference type="OrthoDB" id="1861282at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000413">
        <property type="term" value="P:protein peptidyl-prolyl isomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PTHR45625"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="SSF50891"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0697">Rotamase</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5006528793" description="Peptidyl-prolyl cis-trans isomerase" evidence="2">
        <location>
            <begin position="20"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="3">
        <location>
            <begin position="39"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="186" mass="19792" checksum="707FE416F9FB4A9E" modified="2005-10-11" version="1">MIRILGLVAALIFAVPAFAQPLPAGLDKQNAIVIDTTKGQIVIKLRSDIAPKHAERIKQLARDGFYNNVPFHRVMDGFMAQTGDGKNFNGTGGSKYPNLQAEFSNVPFKRGVVGMARAGSPDSANSQFFICFADASFLNGKYTVVGDVVAGMDVVDKLKKAPAGSSSGTVTDPDKMLKVQVASDVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRQ0</accession>
    <name>Q3SRQ0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein-L-isoaspartate O-methyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">L-isoaspartyl protein carboxyl methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Protein L-isoaspartyl methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Protein-beta-aspartate methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_1780</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315037.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1780"/>
    <dbReference type="EnsemblBacteria" id="ABA05041">
        <property type="protein sequence ID" value="ABA05041"/>
        <property type="gene ID" value="Nwi_1780"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1780"/>
    <dbReference type="eggNOG" id="COG2518">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055432_2_1_5"/>
    <dbReference type="OMA" id="MVDTQVR"/>
    <dbReference type="OrthoDB" id="1948290at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004719">
        <property type="term" value="F:protein-L-isoaspartate (D-aspartate) O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000682">
        <property type="entry name" value="PCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579">
        <property type="entry name" value="PTHR11579"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00030757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="220" mass="23263" checksum="CC42040B5A6EA3A8" modified="2005-10-11" version="1">MSDFSTARGKMVDGQVRPSDVTDLRIIDAMLALPREIFVPGDRRALAYLDLDLDVSEGTSKRYLLKPMVVAKMVQAAEIKSTDNVLVVGCATGYTAALVGKLTDRVTAIESDPALATKASDNLASLGLGHVTVIPARANDGASARAPYDVIILEGATEIVPDALYGQLAPEGRLVGVFAIDGAPRVVIVTRSRSDFGSRPLFNASAPILPGLERPPAFVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRR9</accession>
    <name>Q3SRR9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1761</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05022">
        <property type="protein sequence ID" value="ABA05022"/>
        <property type="gene ID" value="Nwi_1761"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1761"/>
    <dbReference type="eggNOG" id="COG2388">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132888_0_0_5"/>
    <dbReference type="OMA" id="QRVFFHT"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045057">
        <property type="entry name" value="Gcn5-rel_NAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031165">
        <property type="entry name" value="GNAT_YJDJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31435">
        <property type="entry name" value="PTHR31435"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14542">
        <property type="entry name" value="Acetyltransf_CG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51729">
        <property type="entry name" value="GNAT_YJDJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="35"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="123" mass="13722" checksum="5A4EA9C651D4E908" modified="2005-10-11" version="1">MAGRRRSAVVDWLPKTLNVASQAGKNKGCEMSEVVNNMEKHRYELEVDGLVAATYYRFLDGVLVFTHTQVPPELEGKGVGSELVKGALDQVRADKFRASAQCPFVKAWLEKNPDYADLLRPNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRV6</accession>
    <name>Q3SRV6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Lumazine-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1724</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1724"/>
    <dbReference type="EnsemblBacteria" id="ABA04985">
        <property type="protein sequence ID" value="ABA04985"/>
        <property type="gene ID" value="Nwi_1724"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1724"/>
    <dbReference type="eggNOG" id="COG0307">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034388_2_2_5"/>
    <dbReference type="OMA" id="HFVTGHV"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00402">
        <property type="entry name" value="Riboflavin_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001783">
        <property type="entry name" value="Lumazine-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026017">
        <property type="entry name" value="Lumazine-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21098">
        <property type="entry name" value="PTHR21098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00677">
        <property type="entry name" value="Lum_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000498">
        <property type="entry name" value="Riboflavin_syn_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00187">
        <property type="entry name" value="ribE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51177">
        <property type="entry name" value="LUMAZINE_BIND"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="Lumazine-binding" evidence="2">
        <location>
            <begin position="24"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Lumazine-binding" evidence="3">
        <location>
            <begin position="24"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="repeat" description="Lumazine-binding" evidence="2">
        <location>
            <begin position="126"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Lumazine-binding" evidence="3">
        <location>
            <begin position="126"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="232" mass="25186" checksum="B7E736720623EE6B" modified="2005-10-11" version="1">MPVPVPGVYLFRPLRMYWIRKVLMFTGIVTDIGEIESVTLTAQNRLHRLRILCHYDQGTIADGASIACNGACLTVVASGVAAGLTWFDVDVGAETLDVTTAKHWSVGTRLNLERALKIGDELGGHIVAGHVDGIATILTRDDQHGMARFEFSTAPKLARFIAVKGSVTLDGVSLTVNSVRNEAFSVLTIPRTLDLTTLGDWRPNSEVNIEVDLMARYAARLSEMTCVSSTEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS15</accession>
    <name>Q3SS15_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cysteine desulfurase</fullName>
            <ecNumber evidence="4">2.8.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_1665</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[sulfur carrier]-H + L-cysteine = [sulfur carrier]-SH + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:43892"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="EC" id="2.8.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="2.8.1.7" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1665"/>
    <dbReference type="EnsemblBacteria" id="ABA04926">
        <property type="protein sequence ID" value="ABA04926"/>
        <property type="gene ID" value="Nwi_1665"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1665"/>
    <dbReference type="eggNOG" id="COG1104">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003433_0_0_5"/>
    <dbReference type="OMA" id="IIYGQSE"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031071">
        <property type="term" value="F:cysteine desulfurase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016454">
        <property type="entry name" value="Cysteine_dSase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005572">
        <property type="entry name" value="NifS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_5" evidence="7">
        <location>
            <begin position="13"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA04926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="393" mass="41846" checksum="DCC28D0232765247" modified="2005-10-11" version="1">MPYSRNHMQERTYLDWNATTPLRREAREAMAAAWDLFGNPSSVHAEGRRARGLIEDARIIVAEAVGGEPRNVIFTSGGTEANTLALTPVIRKQKHDIDNIIVSSIEHASVLAGARFPPDRMTRLPVDRSGLIDLDALRAMLARQPPTLVSIMLANNETGAIQPVAEAAGLVHEAGGLLHVDAVQAFGKIHIDINELNADFVSLSAHKVGGPKGAGALVLAENRSHPTALIRGGGQERGGRAGTENVPGIAGFGAAVRAAEADRAIDTARIKSLRDRLEDGLRQTRGGVIFSQKVARLPNTTLFGVPTLKAETAVVRFDLEGVAVSSGSACSSGKVQASHVLAAMGYGPEMTRSAIRFSLGWETTDADVDRAIKTWRKLSGALLRGNETGLEPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS61</accession>
    <name>Q3SS61_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phage head-tail adaptor, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1619</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1619"/>
    <dbReference type="EnsemblBacteria" id="ABA04880">
        <property type="protein sequence ID" value="ABA04880"/>
        <property type="gene ID" value="Nwi_1619"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1619"/>
    <dbReference type="eggNOG" id="COG5614">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147810_6_1_5"/>
    <dbReference type="OMA" id="IFRIRYM"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008767">
        <property type="entry name" value="Phage_SPP1_head-tail_adaptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038666">
        <property type="entry name" value="SSP1_head-tail_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05521">
        <property type="entry name" value="Phage_H_T_join"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01563">
        <property type="entry name" value="gp16_SPP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="109" mass="12530" checksum="01AA3DDB1666E498" modified="2005-10-11" version="1">MERRDMRAGKLDQQIAIQRFTNTVDDFGTPVETWTTLATVRAQVIQQSTEEFIRNFGASDETITVFRVRWIDGITTADRVLFDGDSHNIKETKQIGRRAGLELRCQRIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS67</accession>
    <name>Q3SS67_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1613</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04874">
        <property type="protein sequence ID" value="ABA04874"/>
        <property type="gene ID" value="Nwi_1613"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1613"/>
    <dbReference type="eggNOG" id="ENOG50346ZY">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1011677_0_0_5"/>
    <dbReference type="OMA" id="NWIKARI"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="46"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="281" mass="31876" checksum="4ADD32D8727FD91F" modified="2005-10-11" version="1">MDWMNSALGWISIGLSILWPLALLLGRNWIKARIESSVRHGFELKIEAVRSELRANEEKIRSQLREKEQEISTLRQTVLNGTANRQSLFDKRRFEAVEKVWASVNDYSKLLWVSSTIAIMKVEAVAAEASDPKMQRVLAAMGQNPPDLKDLQDAKNERPFLSETAWAYYSAYTSILVGNYTVFKILQTGVSDPLRFINRDGNKEILKAALPHQVGYIEKYGAQGYHHLLGEIQSLLLAELINMLNGKETDRERVENARGLLKVIQRTEDERGQTEHVAAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSE1</accession>
    <name>Q3SSE1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1539</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314806.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1539"/>
    <dbReference type="EnsemblBacteria" id="ABA04800">
        <property type="protein sequence ID" value="ABA04800"/>
        <property type="gene ID" value="Nwi_1539"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1539"/>
    <dbReference type="HOGENOM" id="CLU_1833078_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="68"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="140" mass="15275" checksum="8F2DF48B620A7A7A" modified="2005-10-11" version="1">MSIKIIAYLVAAALIASASLYLANLYQKAGAFDVLSAEHHTLEVRYGCDKRPAISERKLPACLIARGLDAEKAHREEIERQRNEAARAQAKLDADKLAAERAQQSENAIIKAAPASDDGPVPKVLLDAWTRERGRLGISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSH8</accession>
    <name>Q3SSH8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Allophanate hydrolase subunit 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_1502</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314769.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1502"/>
    <dbReference type="EnsemblBacteria" id="ABA04763">
        <property type="protein sequence ID" value="ABA04763"/>
        <property type="gene ID" value="Nwi_1502"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1502"/>
    <dbReference type="eggNOG" id="COG1984">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028967_0_1_5"/>
    <dbReference type="OMA" id="QDLGRSH"/>
    <dbReference type="OrthoDB" id="909676at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003778">
        <property type="entry name" value="CT_A_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02626">
        <property type="entry name" value="CT_A_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00797">
        <property type="entry name" value="AHS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="SSF50891"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00724">
        <property type="entry name" value="urea_amlyse_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AHS2" evidence="4">
        <location>
            <begin position="25"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="339" mass="35045" checksum="DA4EB5CE60D83BF8" modified="2005-10-11" version="1">MSKLVVTAVGPVTSVQDGGRSGAQRYGLPPGGAADRLALAAGNCLVGNPPFAAAIEIGPFSTSFTVREGRVRVALTGAARSADVSGRPVSFNESCTFGDGDSLTLGVARDGTFSYLAIEGGVKGEPMFGSLAVLARAGLGSPFPRPLQAGDVLDVEAATTGAEHGIELPGQDDGPIRIVMGPQDDEFGEATGLFLGSEWKISATSDRMGYRLEGPIIEHLHGHNIVSDGTVNGSIQIPGNGQPIVLMPDRGTSGGYPKIATVMTADLGRFAQIPVGRGFRFKSVTVAEAQAEARAMADLLRTLPGRVRAVRNTDIDDALRNANIAGTAVNAFDSGTWQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSJ0</accession>
    <name>Q3SSJ0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lipoprotein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1490</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314758.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04751">
        <property type="protein sequence ID" value="ABA04751"/>
        <property type="gene ID" value="Nwi_1490"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1490"/>
    <dbReference type="eggNOG" id="ENOG5032UZW">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155147_0_0_5"/>
    <dbReference type="OMA" id="RISARVM"/>
    <dbReference type="OrthoDB" id="1714278at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229253" evidence="1">
        <location>
            <begin position="25"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="123" mass="12811" checksum="BE9A58254FE6D355" modified="2005-10-11" version="1">MKIRTVLLCAALGGCASSSNEITAAYVSPVMYQGYTCQQLALEAQGVSARAAALSGAQDKQRTNDAVATTAAVIIFWPAAFLVGGDKQTAAELGQMKGQMNAIEQASIAKKCGIEFHRGPSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSK7</accession>
    <name>Q3SSK7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phage terminase, small subunit, putative, P27</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1473</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314741.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1473"/>
    <dbReference type="EnsemblBacteria" id="ABA04734">
        <property type="protein sequence ID" value="ABA04734"/>
        <property type="gene ID" value="Nwi_1473"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1473"/>
    <dbReference type="eggNOG" id="COG3747">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107958_4_1_5"/>
    <dbReference type="OMA" id="QSEAMNR"/>
    <dbReference type="OrthoDB" id="1570231at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006448">
        <property type="entry name" value="Phage_term_ssu_P27"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05119">
        <property type="entry name" value="Terminase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01558">
        <property type="entry name" value="sm_term_P27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="131" mass="14644" checksum="384DE2D2408E840F" modified="2005-10-11" version="1">MKGTKPHLRTDSAAMKRAPAVPKWLSADAKREWKRILPDLIERRILTDADLGSLENYCIAIGQVREMERILQTEGHVYGAESGLLKRHPAGVILSDAMTRARLLAVEMGLTPVSRSRPAIRDDDEEDSLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSM0</accession>
    <name>Q3SSM0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lactamase_B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314728.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1460"/>
    <dbReference type="EnsemblBacteria" id="ABA04721">
        <property type="protein sequence ID" value="ABA04721"/>
        <property type="gene ID" value="Nwi_1460"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1460"/>
    <dbReference type="eggNOG" id="COG1235">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044538_2_1_5"/>
    <dbReference type="OMA" id="CGPDFRQ"/>
    <dbReference type="OrthoDB" id="1712770at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="51"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="267" mass="29711" checksum="D2B2DDD92CCB7991" modified="2005-10-11" version="1">MTLTLTILGCGSSAGVPRPAMGWGACDPANPKNRRRRCSLMAERTSEEGTTRVVIDTSPDLREQLIDANVDHIDAVFLSHEHADQTHGIDDLRSVVLHQGRRIPVYLNKSTAVDVMQRFAYCFETPEGSFYPPILEQRSIEAGESRTINGSGGALTLSAFLVRHGQITALGYRIGDAAYTPDLCDIPPESWPALKNLDLWIVDALRYAQHPTHFSVADALSWIDRFKPRRAVITNMHSDLDYEILRRELPTNVTPAYDGMRLTIDPA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-07-24" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSN8</accession>
    <name>ISPDF_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Bifunctional enzyme IspD/IspF</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="1">2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase</fullName>
                <ecNumber evidence="1">2.7.7.60</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="1">4-diphosphocytidyl-2C-methyl-D-erythritol synthase</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="1">MEP cytidylyltransferase</fullName>
                <shortName evidence="1">MCT</shortName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="1">2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase</fullName>
                <shortName evidence="1">MECDP-synthase</shortName>
                <shortName evidence="1">MECPP-synthase</shortName>
                <shortName evidence="1">MECPS</shortName>
                <ecNumber evidence="1">4.6.1.12</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="1" type="primary">ispDF</name>
        <name type="ordered locus">Nwi_1442</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Bifunctional enzyme that catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) (IspD), and catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP) (IspF).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-C-methyl-D-erythritol 4-phosphate + CTP + H(+) = 4-CDP-2-C-methyl-D-erythritol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13429"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:57823"/>
            <dbReference type="ChEBI" id="CHEBI:58262"/>
            <dbReference type="EC" id="2.7.7.60"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4-CDP-2-C-methyl-D-erythritol 2-phosphate = 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + CMP</text>
            <dbReference type="Rhea" id="RHEA:23864"/>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
            <dbReference type="ChEBI" id="CHEBI:58483"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="4.6.1.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the IspD/TarI cytidylyltransferase family. IspD subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the IspF family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.60" evidence="1"/>
    <dbReference type="EC" id="4.6.1.12" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314711.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSN8"/>
    <dbReference type="SMR" id="Q3SSN8"/>
    <dbReference type="STRING" id="323098.Nwi_1442"/>
    <dbReference type="EnsemblBacteria" id="ABA04703">
        <property type="protein sequence ID" value="ABA04703"/>
        <property type="gene ID" value="Nwi_1442"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1442"/>
    <dbReference type="eggNOG" id="COG0245">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1211">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042800_2_5_5"/>
    <dbReference type="OMA" id="TGYDVHA"/>
    <dbReference type="OrthoDB" id="525632at2"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00093"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00095"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008685">
        <property type="term" value="F:2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050518">
        <property type="term" value="F:2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02516">
        <property type="entry name" value="CDP-ME_synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00554">
        <property type="entry name" value="MECDP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00108">
        <property type="entry name" value="IspD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01520">
        <property type="entry name" value="IspDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00107">
        <property type="entry name" value="IspF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001228">
        <property type="entry name" value="IspD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026596">
        <property type="entry name" value="IspD/F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034683">
        <property type="entry name" value="IspD/TarI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018294">
        <property type="entry name" value="ISPD_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003526">
        <property type="entry name" value="MECDP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020555">
        <property type="entry name" value="MECDP_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036571">
        <property type="entry name" value="MECDP_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43181">
        <property type="entry name" value="PTHR43181"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01128">
        <property type="entry name" value="IspD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02542">
        <property type="entry name" value="YgbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69765">
        <property type="entry name" value="SSF69765"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00453">
        <property type="entry name" value="ispD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00151">
        <property type="entry name" value="ispF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01295">
        <property type="entry name" value="ISPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01350">
        <property type="entry name" value="ISPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000296750" description="Bifunctional enzyme IspD/IspF">
        <location>
            <begin position="1"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase" evidence="1">
        <location>
            <begin position="235"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="241"/>
            <end position="243"/>
        </location>
        <ligand>
            <name>4-CDP-2-C-methyl-D-erythritol 2-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="267"/>
            <end position="268"/>
        </location>
        <ligand>
            <name>4-CDP-2-C-methyl-D-erythritol 2-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="289"/>
            <end position="291"/>
        </location>
        <ligand>
            <name>4-CDP-2-C-methyl-D-erythritol 2-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="365"/>
            <end position="368"/>
        </location>
        <ligand>
            <name>4-CDP-2-C-methyl-D-erythritol 2-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="372"/>
        </location>
        <ligand>
            <name>4-CDP-2-C-methyl-D-erythritol 2-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="375"/>
        </location>
        <ligand>
            <name>4-CDP-2-C-methyl-D-erythritol 2-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="19"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="26"/>
        </location>
    </feature>
    <feature type="site" description="Positions MEP for the nucleophilic attack" evidence="1">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <feature type="site" description="Positions MEP for the nucleophilic attack" evidence="1">
        <location>
            <position position="213"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01520"/>
        </source>
    </evidence>
    <sequence length="398" mass="42115" checksum="2A51DA2EB5BC0930" modified="2005-10-11" version="1">MPSSKRTAAIIVAAGRGLRAGAGGPKQYRTIGGRTVISRAMEAFCQHPDVFAVQPVLNPDDLSMFNQAAAQFRYRPPANGGATRQASVRAGLEALAADAPDIVLIHDAARPFVTPALITRAIDAADKAGAAVPAIAVTDTIKQVDESGAVNATPDRAKLRIAQTPQAFHFDMILDAHRRAAREGRDDFTDDAALAEWVGLTVATFEGDAANMKLTTPEDFVREEARLAAALGDIRTGTGYDVHAFGEGDHLMLCGVKVPHNCGFLAHSDGDVGLHALVDAILGALADGDIGSHFPPSDPQWKGAASDKFLKYAVDRVTARGGRVANLEVTMICQQPKIGPLRDQMRARIADITGVAISRIAVKATTSERLGFTGREEGIAATASATIRLPWNDKGRDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSS1</accession>
    <name>Q3SSS1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, MerR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1409</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1409"/>
    <dbReference type="EnsemblBacteria" id="ABA04670">
        <property type="protein sequence ID" value="ABA04670"/>
        <property type="gene ID" value="Nwi_1409"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1409"/>
    <dbReference type="eggNOG" id="COG0789">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045945_1_0_5"/>
    <dbReference type="OMA" id="ACREMLD"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13411">
        <property type="entry name" value="MerR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH merR-type" evidence="2">
        <location>
            <begin position="16"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="206" mass="22961" checksum="D79C97725309C57C" modified="2005-10-11" version="1">MAQERHLDKAPDAFRTISEVAADLDIPQHVLRFWETRFSQIKPMKRSGGRRYYRPDDVDLLRGIRHLLYSEGYTIRGVQRILKEHGIKSVQGLTEGSSAAAFGAIEDDIRNTLGEIDEEESGYETEDTEDDEDDGDDGDNGPREREPGVHRPLRHDLDEVLWSGPDSGAIPGVAAAAIDLTKLKDVLAELVACKQLLDEALRDGEP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-05-25" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSY4</accession>
    <name>RL11_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplK</name>
        <name type="ordered locus">Nwi_1346</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the ribosomal stalk of the 50S ribosomal subunit. Interacts with L10 and the large rRNA to form the base of the stalk. L10 forms an elongated spine to which L12 dimers bind in a sequential fashion forming a multimeric L10(L12)X complex.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">One or more lysine residues are methylated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314625.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSY4"/>
    <dbReference type="SMR" id="Q3SSY4"/>
    <dbReference type="STRING" id="323098.Nwi_1346"/>
    <dbReference type="EnsemblBacteria" id="ABA04607">
        <property type="protein sequence ID" value="ABA04607"/>
        <property type="gene ID" value="Nwi_1346"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1346"/>
    <dbReference type="eggNOG" id="COG0080">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074237_2_1_5"/>
    <dbReference type="OMA" id="CKQFNAK"/>
    <dbReference type="OrthoDB" id="1702697at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00349">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_L11/L12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036796">
        <property type="entry name" value="Ribosomal_L11/L12_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006519">
        <property type="entry name" value="Ribosomal_L11_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_L11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036769">
        <property type="entry name" value="Ribosomal_L11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_L11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="PTHR11661"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46906">
        <property type="entry name" value="SSF46906"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="SSF54747"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01632">
        <property type="entry name" value="L11_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0488">Methylation</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000258176" description="50S ribosomal protein L11">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00736"/>
        </source>
    </evidence>
    <sequence length="142" mass="15324" checksum="D9638316987E751C" modified="2005-10-11" version="1">MAKKVTGYLKLQVPAGAANPSPPIGPALGQRGLNIMEFCKAFNAQTQKEEKNTPIPVVITIYADRSFTFEMKTPPMAYFLKQAAKIQSGSKTPGRDAAGTLTRAQVREIAEKKMKDLNCDTVEAAMSMVEGSARSMGLQVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST01</accession>
    <name>Q3ST01_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GTP cyclohydrolase 1</fullName>
            <ecNumber evidence="8">3.5.4.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">GTP cyclohydrolase I</fullName>
            <shortName evidence="8">GTP-CH-I</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">folE</name>
        <name evidence="11" type="ordered locus">Nwi_1329</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>GTP + H2O = 7,8-dihydroneopterin 3'-triphosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17473"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58462"/>
            <dbReference type="EC" id="3.5.4.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Cofactor biosynthesis; 7,8-dihydroneopterin triphosphate biosynthesis; 7,8-dihydroneopterin triphosphate from GTP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homopolymer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Toroid-shaped homodecamer, composed of two pentamers of five dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the GTP cyclohydrolase I family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.16" evidence="8"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314608.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1329"/>
    <dbReference type="EnsemblBacteria" id="ABA04590">
        <property type="protein sequence ID" value="ABA04590"/>
        <property type="gene ID" value="Nwi_1329"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1329"/>
    <dbReference type="eggNOG" id="COG0302">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049768_2_2_5"/>
    <dbReference type="OMA" id="CEHMCMS"/>
    <dbReference type="OrthoDB" id="1632367at2"/>
    <dbReference type="UniPathway" id="UPA00848">
        <property type="reaction ID" value="UER00151"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003934">
        <property type="term" value="F:GTP cyclohydrolase I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035998">
        <property type="term" value="P:7,8-dihydroneopterin 3'-triphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.286.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00223">
        <property type="entry name" value="FolE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043134">
        <property type="entry name" value="GTP-CH-I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001474">
        <property type="entry name" value="GTP_CycHdrlase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018234">
        <property type="entry name" value="GTP_CycHdrlase_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020602">
        <property type="entry name" value="GTP_CycHdrlase_I_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109">
        <property type="entry name" value="PTHR11109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01227">
        <property type="entry name" value="GTP_cyclohydroI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00063">
        <property type="entry name" value="folE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00859">
        <property type="entry name" value="GTP_CYCLOHYDROL_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6 8 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="GTP_cyclohydroI" evidence="10">
        <location>
            <begin position="59"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="17"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA04590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="241" mass="26917" checksum="C16F631A020312CF" modified="2005-10-11" version="1">MDALIKPLRSGKPSDINKASDIKSSNAKPAGFSPAQLDPSEFMAAVQPNRQRPSRHEAEDAVRTLLAYIGENPDREGLLDTPRRVIESYDEIYQGYHQCPAEVLNRTFGETAGYDDFVLVRDIQFTSQCEHHMMPFYGKAHIAYTPVERVVGLSKLARLTDIFARRLQTQEHMTAQIAAAVDEVLKPRGVAVMIEAEHTCMSVRGVAKHGAMTVTSRFTGVFRDDPAEQARFLSMVRGQPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST89</accession>
    <name>Q3ST89_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aminotransferase</fullName>
            <ecNumber evidence="2">2.6.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.6.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314531.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1240"/>
    <dbReference type="EnsemblBacteria" id="ABA04502">
        <property type="protein sequence ID" value="ABA04502"/>
        <property type="gene ID" value="Nwi_1240"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1240"/>
    <dbReference type="eggNOG" id="COG0436">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_4_0_5"/>
    <dbReference type="OMA" id="SQGANQY"/>
    <dbReference type="OrthoDB" id="1462898at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="1">
        <location>
            <begin position="24"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="383" mass="42521" checksum="1BF015E5751C3BC0" modified="2005-10-11" version="1">MNKVFADLPVTIFETMSQLARDHDAINLGQGFPDDPGPEDIRRAAADAVINGYNQYPSMMGIPELRQAVAAHYRHWHGLAADPASEVMVTSGGTEALTSAILATVEPGDEVVVFQPFYDSYLPIIRQAGGVPRLVRLEPPHWRLTEDALRDAFTPRTRVVLFNNPLNPAAVVYARNELELLARFCQEFDAVAICDEVWEHVVFDGREHIPLITVPGMRDRTIKIGSAGKIFSLTGWKVGFVCAAPPLLRVIAKVHQFLTFTTPPNLQAAVAGGLAKPDDYFFEMRRDLARRRDRLTRGLEDIGFPVLPSQGTYFLTVDLAPLGLNETDHVFCRRIVVDHKVAAIPVSAFYEKDAVTSVVRFCFAKKDATLDAALERLSDAVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STB9</accession>
    <name>Q3STB9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">4-alpha-glucanotransferase</fullName>
            <ecNumber evidence="3 7">2.4.1.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5 7">Amylomaltase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6 7">Disproportionating enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_1210</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Transfers a segment of a (1-&gt;4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1-&gt;4)-alpha-D-glucan.</text>
            <dbReference type="EC" id="2.4.1.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the disproportionating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.25" evidence="3 7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1210"/>
    <dbReference type="CAZy" id="GH77">
        <property type="family name" value="Glycoside Hydrolase Family 77"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04472">
        <property type="protein sequence ID" value="ABA04472"/>
        <property type="gene ID" value="Nwi_1210"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1210"/>
    <dbReference type="eggNOG" id="COG1640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022072_1_1_5"/>
    <dbReference type="OMA" id="QYPNWRL"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004134">
        <property type="term" value="F:4-alpha-glucanotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102500">
        <property type="term" value="F:beta-maltose 4-alpha-glucanotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003385">
        <property type="entry name" value="Glyco_hydro_77"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32438">
        <property type="entry name" value="PTHR32438"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02446">
        <property type="entry name" value="Glyco_hydro_77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00217">
        <property type="entry name" value="malQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA04472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="663" mass="74047" checksum="477A951694D684AF" modified="2005-10-11" version="1">MPSGSGSGMTIHSKAQDLGINLDFVDGLGRHHLLDDVRLRPIVDGLPSPPHHRFLKHPVVIVTNADDFSVPVGAEANGVFWRLVEGHTVLAQGYISSARIDLPRWISVGTYRLELESEKGGILDSVPVLVTPERAYQGSFERVWILSVQLYALTSSRNWGIGDFTDLVSLIELTSDWGCHGIGLNPLHALFDDRPLECSPYAPNSRLFLNPLYVDPERVPNCSRVWLKRQREQLDRVREGELVDYAAVAELKFAAFRHAFSRFRRDHAAQAEFDLFREERGQLLKRYACFEVLRRQFGPSPWWSWPDEWRRPTDAGIQAISSGSRREEVKFVEFMQWCAASQLQSCADLAAERGMTVGLYLDVAVGVQAGGFDAWNEQTSISRALSVGAPPDQLNTAGQDWGLAGFNAAGLEASDFAPFREMLRASMRFAGAIRLDHVFGLSRLYLIPAGGSPLQGAYVKMPLQALLGVIAQESVSQRCIIIGEDLGTVPDGFREKLADWGVWSYKVMMFERGNDGAFIEASKYAENALVTFTTHDLASFAGWQTHSDLRVKRELGIEPGETDAERTDARHAFEWKLGEEHIAVKNFIGAVEFLARTPSRILAIALDDLIELRDQPNIPGTLNEHPNWRRRTPVRLEDLGGCIDLDALRHALRGRCNSVHDNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STH7</accession>
    <name>Q3STH7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_1152</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314444.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1152"/>
    <dbReference type="EnsemblBacteria" id="ABA04414">
        <property type="protein sequence ID" value="ABA04414"/>
        <property type="gene ID" value="Nwi_1152"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1152"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_2_7_5"/>
    <dbReference type="OMA" id="GWNFLVQ"/>
    <dbReference type="OrthoDB" id="1558246at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="270"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="300"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="343"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="373"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="398"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="441"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="485"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="7">
        <location>
            <begin position="52"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA04414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="516" mass="54047" checksum="18E0E01F8CD451B0" modified="2005-10-11" version="1">MNTYERLGRGPSGPPISSGEGAARPGFAEGEGDAPPVVVSPAQPGRSEIRTILVSLMLTMFLAALDQTIVATALPTIGRQFHDVTTLAWIITAYLLASTAVAPLFGTLSDIYGRRAMIVTALGLFIAGSVLCALAQNMATLIVARGVQGIGGGGILPLVQTIISDVVTPRERGQYQAYFSGVWTAAGIAGPVLGGVFAEHLHWSMIFWINVPLGLVSLALLLPKMGGIPAFHRRRKVDWTGGLLLMTSAVVFMLVLTWGGNRFAWFSREIVAMLGISLVLAAVFVWHARKASEPFLPLSLIGGSVVPYAIAASALALSAIIGITVHLPLYYQAVHHLGVSESGLALIPIAAVSTGGAMLAGRAMTHTLHYKRIAIVGTAFAAVMAAIMAAVTMPLWPLLAMLSLFAFGLGTIFPVSVVSLQNAVMREQVGTVTGAMNFFRALACSFVVAAFAAILLMTLGTDAMIGHEGGGRLSAIQASDLTRAFRYVFAATAAMMAAAALLLVRMEERPLAGPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STH8</accession>
    <name>Q3STH8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0260 protein Nwi_1151</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1151</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0260 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1151"/>
    <dbReference type="EnsemblBacteria" id="ABA04413">
        <property type="protein sequence ID" value="ABA04413"/>
        <property type="gene ID" value="Nwi_1151"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1151"/>
    <dbReference type="eggNOG" id="COG2983">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109769_0_0_5"/>
    <dbReference type="OMA" id="GRCCLHK"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00676">
        <property type="entry name" value="UPF0260"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005358">
        <property type="entry name" value="Puta_zinc/iron-chelating_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008228">
        <property type="entry name" value="UCP006173"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37421">
        <property type="entry name" value="PTHR37421"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03692">
        <property type="entry name" value="CxxCxxCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="37"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="213" mass="23997" checksum="76D894BED937FA94" modified="2005-10-11" version="1">MAGRDVSPALRRPSSYGARLREVPAKPIDGDRHSLAQASAHSMTERPAQSSEQKGFFWKTKSLDEMSPAEWESLCDGCGRCCLEKLEDEDTGDIYFTHVSCRLLDAGLCACKDYPKRSEQVPDCVRLTPENVRTLNWLPPSCGYRLVAEGRDLYWWHPLISGDPDTVHEAGVSVRGRVRGTEDEIPDSELEDHIVQWPVLLPKRARLRKPPGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STM8</accession>
    <name>Q3STM8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1101</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314394.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1101"/>
    <dbReference type="EnsemblBacteria" id="ABA04363">
        <property type="protein sequence ID" value="ABA04363"/>
        <property type="gene ID" value="Nwi_1101"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1101"/>
    <dbReference type="eggNOG" id="COG1344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019929_0_0_5"/>
    <dbReference type="OMA" id="GMGRTIV"/>
    <dbReference type="OrthoDB" id="1788350at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009288">
        <property type="term" value="C:bacterial-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001492">
        <property type="entry name" value="Flagellin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42792">
        <property type="entry name" value="PTHR42792"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="501" mass="51079" checksum="21D6A0E5441F5C25" modified="2005-10-11" version="1">MAINGISFGNTILGGSVRNLKAQMADLQNQLTSGKKSTTYSGMGANGGFAIAARAQLSNIAAYTDTMMKINTNINVANTALQALSDIGTQLQNAALSGSQALNSAGQTAGQQTAVAQFSSVVGILNTQAGDRYIFSGSEIYTQPVVSSDLILNGDGAGRAGLKQVISERRQADLGASELGRLEISSPTATSVAIAEDASPSVYGMKLQSVSSSLTGATVTGPAGAPPAISIDLGVTNPADGDTVSFTFDLPDGTSETVQLTASSEAPTPAGSFAIGATPTATAANLNAALTASIGALAGTSLVAASAVQASRDFFGQPPQRVDVIPPATLSTATAAVNGTAADTVQWYTGDEGTGSARATSVVRVDASVTVQYGMRANEDAIRSLMESVALLAAVTTSPADPNAADQISALNARVARNLSVEPGSQNIRDIQVDLANAQTMMKDATARQKLTQATMQNIIDQAENISTDEVASQLLALQTDLQASYQTTAMLSQLSLVKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STP6</accession>
    <name>Q3STP6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Metallophosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1083</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallophosphoesterase superfamily. YfcE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1083"/>
    <dbReference type="EnsemblBacteria" id="ABA04345">
        <property type="protein sequence ID" value="ABA04345"/>
        <property type="gene ID" value="Nwi_1083"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1083"/>
    <dbReference type="eggNOG" id="COG2908">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061126_1_0_5"/>
    <dbReference type="OMA" id="YANDGDW"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043461">
        <property type="entry name" value="LpxH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34990">
        <property type="entry name" value="PTHR34990"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos_2" evidence="2">
        <location>
            <begin position="24"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="278" mass="31337" checksum="3C4DAEA5F2C0214B" modified="2005-10-11" version="1">MASCYPMLMGSYAMDDVKPDRRFRTLFISDVHLGASGSQADQLLDFLKYHDAATIYLVGDIVDGWALKSSWHWPQSHNDFVQKMLRKARKGATITYVPGNHDEFLRSYYGTHFGGIEVVENAIHTGVDGRRYLVIHGDIFDLVVQNARWLALLGDKAYDFAIRVNRLVNAFRRSFGFPYWSLSKWAKFKVKNAVNYIGAFEQTLASEAGRHGADGVICGHIHHATIRSEHGIRYMNCGDWVESCTALAEHEDGRFEIITWADPLPRSSPVLPVSARAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STV8</accession>
    <name>Q3STV8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1021</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04283">
        <property type="protein sequence ID" value="ABA04283"/>
        <property type="gene ID" value="Nwi_1021"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1021"/>
    <dbReference type="eggNOG" id="ENOG5031EIG">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_206375_0_0_5"/>
    <dbReference type="OMA" id="WTKDDVK"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="58" mass="6442" checksum="742C0BA053B76084" modified="2005-10-11" version="1">MAKKTKTIRREWTKDDVKTLKLLAKQKAGVAKIAKKLKRTPGATAAKAYTLGVSLRMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STW0</accession>
    <name>Q3STW0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1019</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04281">
        <property type="protein sequence ID" value="ABA04281"/>
        <property type="gene ID" value="Nwi_1019"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1019"/>
    <dbReference type="HOGENOM" id="CLU_1530961_0_0_5"/>
    <dbReference type="OrthoDB" id="2208663at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="175" mass="19805" checksum="C3B963BD503D2023" modified="2005-10-11" version="1">MESGEPRPTRFNPRDREWFLSQKSRPTRSGLSYGTGNALSGAAAVTDFFLPLRFPGRIIFLYGIPNFGLSPPLILRLLHLRPAAAVLLLSAIFFVYRSLTRISRIWIRRVGGTRIAGRTQVIGSRRRMATLRILMHIFTDTGPRFTIIAHRMRSFADCDDLNNSSASPSFLMQHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STW6</accession>
    <name>Q3STW6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phasin_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1013</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314313.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1013"/>
    <dbReference type="EnsemblBacteria" id="ABA04275">
        <property type="protein sequence ID" value="ABA04275"/>
        <property type="gene ID" value="Nwi_1013"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1013"/>
    <dbReference type="eggNOG" id="COG5490">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119062_1_1_5"/>
    <dbReference type="OMA" id="AKEFGSM"/>
    <dbReference type="OrthoDB" id="1750408at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018968">
        <property type="entry name" value="Phasin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010234">
        <property type="entry name" value="Phasin_subfam-2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09361">
        <property type="entry name" value="Phasin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01985">
        <property type="entry name" value="phasin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phasin_2" evidence="2">
        <location>
            <begin position="30"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="126" mass="13820" checksum="DFC7488480AAB06D" modified="2005-10-11" version="1">MSEQGRYSEAANETRSMAEAGLEQARKALENFLAGAEQTANSIEGRNEAVRDSVRDISSKAISFAQQNMTASLDYAEKLVRARDLSEVMRLNTDYVQDQMRALTEQASEIGQSMGRAALGEGKPQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU93</accession>
    <name>Q3SU93_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0884</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314189.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0884"/>
    <dbReference type="EnsemblBacteria" id="ABA04148">
        <property type="protein sequence ID" value="ABA04148"/>
        <property type="gene ID" value="Nwi_0884"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0884"/>
    <dbReference type="HOGENOM" id="CLU_538435_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010567">
        <property type="entry name" value="Clostridium_P47"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06597">
        <property type="entry name" value="Clostridium_P47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="506" mass="52274" checksum="3EE373BBAE1FD084" modified="2005-10-11" version="1">MELKKDISNPNVFSIAEGVVYDLPEAPLRAPEPAPVSASTFGWTLVNGLVEAEVNTAWAADQPAAVTEFSFDLGDGISVSGTFGKVSIHDGGPQGLQGSGRYLYLAMPLIKGVVTRSGASETVSPGTFVVQAPLAFSPPIPASAAQTASGTQHVLGVDWTETASSPAIIDYLYSGAGSLGPVPSALLRAGFGAWLQRAAADGKLPKLNSATVTINKTFSGFSWLQLAQASYAYQGSGEDAPGVLGIMGGTTSPGAFHVLAPDVIPSGGSAGVLIGSQAFVQDVLLPAATAAFDGASPDEFELLNDDTSISLKSGKTLKLPAAVVESGNYDLTVTQFSITLQDRQIVSFVEAKTEVEPGVTLYVDVTLYNGLELITKEDGSRTLNYTVTRAADASHRIHNSTGAIIGKVLMSVILTLIFSVSGAVAKNLGDKIMLLILATVIKGGITAAQNILPDLLKNGVKVALPSIDEMINTLESSVAWAGKNGKSFAASGAQLNESVQISGSFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUE2</accession>
    <name>Q3SUE2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Recombinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0834</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0834"/>
    <dbReference type="EnsemblBacteria" id="ABA04099">
        <property type="protein sequence ID" value="ABA04099"/>
        <property type="gene ID" value="Nwi_0834"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0834"/>
    <dbReference type="eggNOG" id="COG1961">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1004105_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038109">
        <property type="entry name" value="DNA_bind_recomb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011109">
        <property type="entry name" value="DNA_bind_recombinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07508">
        <property type="entry name" value="Recombinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="SSF53041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51737">
        <property type="entry name" value="RECOMBINASE_DNA_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Recombinase" evidence="1">
        <location>
            <begin position="91"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="277" mass="31345" checksum="725AFA3AC9D4ABA0" modified="2005-10-11" version="1">MIADGLKRRFDLVLTESLDRLSRDQEDIAGFYKRMRFVGINIVTLSEGEVSELHIGLKGTMGALYLKDLADKTRRGLRGRVEDGKSGGGLYYGYDVVKQFDANGEAIRGDRTINQAEADVVRRIFADYLAGQSSRTIAMTLNREGVSGPQGSEWGPSTIHGNPKRGVGILNNELYVGKLVWNRLRYIKDPDTGKRVSRLNPEGEWVIQDVPELRVARIWRRSITKRSPPYTGTYRARKPRPRLRRPSGLWSAGWTSFRKAERWQSSCAATWRRSSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUH6</accession>
    <name>Q3SUH6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0800</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314113.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0800"/>
    <dbReference type="EnsemblBacteria" id="ABA04065">
        <property type="protein sequence ID" value="ABA04065"/>
        <property type="gene ID" value="Nwi_0800"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0800"/>
    <dbReference type="eggNOG" id="ENOG502Z80P">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051803_0_0_5"/>
    <dbReference type="OMA" id="YGREALP"/>
    <dbReference type="OrthoDB" id="922344at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044000">
        <property type="entry name" value="Phage_tube_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18906">
        <property type="entry name" value="Phage_tube_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="313" mass="32987" checksum="875313B9BED8FC72" modified="2005-10-11" version="1">MARARGANAVMAAAFESTYGTAPVSGFKKLPFVSAALGDEQGLIPSDLLGYGREPLPPSRDVVNNEGDVVVPVDLRNFGQWLKLAMGAPSSVEDTGVYTHTFVSGALTLPSMAVQIGLPEVPSYGVNVGIRANTMQIQLQRSGLLNATMGLIAQGETKFSTSQAGTPTEAVIERFSHFQGEVKREGTALGNVVSAELTYSNGLDKVEVIRPDGRIADADPGMVAVTGSIGIRFADMTLLDQASSGEPCELSFGWTIDADRSLLFTLHSVFLPRAKQPVQGPAGVQATFAWQAAKDPILGKTCTVVLVNDVAAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUI5</accession>
    <name>Q3SUI5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0791</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314104.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0791"/>
    <dbReference type="EnsemblBacteria" id="ABA04056">
        <property type="protein sequence ID" value="ABA04056"/>
        <property type="gene ID" value="Nwi_0791"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0791"/>
    <dbReference type="eggNOG" id="COG5301">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102093_0_0_5"/>
    <dbReference type="OMA" id="GPTAYYA"/>
    <dbReference type="OrthoDB" id="1612055at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021251">
        <property type="entry name" value="DUF2793"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10983">
        <property type="entry name" value="DUF2793"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="232" mass="23976" checksum="DA836F1F0D912DC4" modified="2005-10-11" version="1">MATPNLGLPVLAQGQAQKEITHNDALLRLDALVQTSIKSRALATPPGSPANGERWIVPSGATGAWAGQTDKIAFWREGAWAFFAPGVGWRVHVEDERLTVVWTDGAWRDRIVGTANGGAIRLVALEEELTLTGAFVDAATAVIADRMIVLAVASLTTQAITGATSYGVGVAGNNSQFGGSLGIALGSTNIGVIGPTAFYSNTPIRVTAAGSNFTGGKVRVVLYALAFTAPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUL8</accession>
    <name>Q3SUL8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0758</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04023">
        <property type="protein sequence ID" value="ABA04023"/>
        <property type="gene ID" value="Nwi_0758"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0758"/>
    <dbReference type="HOGENOM" id="CLU_2586148_0_0_5"/>
    <dbReference type="OMA" id="FRIHRDF"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="85" mass="9361" checksum="C3BCF3FFE2170067" modified="2005-10-11" version="1">MDVCFSRSVGEHNHFAVNDLILGNKLRSSFRIDLDLRVRESFLDRADSRVLVGDGDKDEFPARRSAGASTGPHGADFPTFDHGSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUM6</accession>
    <name>Q3SUM6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ribonuclease BN</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0750</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314069.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0750"/>
    <dbReference type="EnsemblBacteria" id="ABA04015">
        <property type="protein sequence ID" value="ABA04015"/>
        <property type="gene ID" value="Nwi_0750"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0750"/>
    <dbReference type="eggNOG" id="COG1295">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045539_4_3_5"/>
    <dbReference type="OMA" id="KQPKFRW"/>
    <dbReference type="OrthoDB" id="1421045at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017039">
        <property type="entry name" value="Virul_fac_BrkB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30213">
        <property type="entry name" value="PTHR30213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03631">
        <property type="entry name" value="Virul_fac_BrkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF035875">
        <property type="entry name" value="RNase_BN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="26"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="135"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="207"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="243"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="297" mass="32372" checksum="2005D5C0C7AAE091" modified="2005-10-11" version="1">MRQIRDLYTIGTDAFYTFLADDGWAIASHIALSALMALFPFLIVLTALAGFFGSKELADQAADLMLQIWPDQVANTLSREIHDVLTTSRGDALTVGLVLAVYFASNGVESLRVALNRAYAVIEPRRWYWLRLESIGYTLVAAVTTLAMAFLIVLGPLIIAAARRHVPLIVESNESLLNIARYGITILAMFTALVILHAWLPAGRRSFVQIIPGIIFTMLASLISGVGFGLYLARFANNYVTMYAGLASVIIALVFLYFIAAIFVFGGELNAAIIKSRLPRGVSLQAAQSLEPGEKQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUQ9</accession>
    <name>Q3SUQ9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Thioredoxin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_0716</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0716"/>
    <dbReference type="EnsemblBacteria" id="ABA03982">
        <property type="protein sequence ID" value="ABA03982"/>
        <property type="gene ID" value="Nwi_0716"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0716"/>
    <dbReference type="eggNOG" id="COG0526">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_11_0_5"/>
    <dbReference type="OMA" id="VPCRKEM"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015036">
        <property type="term" value="F:disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013740">
        <property type="entry name" value="Redoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08534">
        <property type="entry name" value="Redoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="5">
        <location>
            <begin position="76"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA03982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="223" mass="23617" checksum="C4D8B76895FC1FED" modified="2005-10-11" version="1">MTDTPTPPHRAESRFPIGPLVAVAIVVGATVALLGIGHLRHAKSAVNALAPCGPAMELSRKLAPQATGEVASLTMASKPRRMPDLAFLDGEGQPMKLSDWKGKTVLLNLWATWCVPCRTEMPALDRLQGKLGGDKFAVVAINIDTRDSDKPKAFLKDANLTHLGNFHDEKASVFQELKSVGMAVGMPTSVLIDGQGCELGNMAGPAEWDSEDAIKLINAAVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUS0</accession>
    <name>Q3SUS0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Outer membrane transport energization protein TonB</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="6">TonB protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_0705</name>
        <name evidence="7" type="ordered locus">Nwi_2038</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="6 8" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="6">Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0705"/>
    <dbReference type="EnsemblBacteria" id="ABA03971">
        <property type="protein sequence ID" value="ABA03971"/>
        <property type="gene ID" value="Nwi_0705"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05297">
        <property type="protein sequence ID" value="ABA05297"/>
        <property type="gene ID" value="Nwi_2038"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0705"/>
    <dbReference type="KEGG" id="nwi:Nwi_2038"/>
    <dbReference type="eggNOG" id="COG0810">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076333_2_0_5"/>
    <dbReference type="OMA" id="AGFKRYP"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006260">
        <property type="entry name" value="TonB/TolA_C"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01352">
        <property type="entry name" value="tonB_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="74"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="88"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA03971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="267" mass="28184" checksum="26A3E924C77AC930" modified="2005-10-11" version="1">MMKIDWRDPDGRAGDDVNRAAALRWTAAGLAVVALHAGGIWMALHWPAAAEAPGDPPAAIMMELAPMAVAPEAPQQDVAPGPEMVEAEEQVDPDKPIEEKPDPEPTPPEVKEAEVKLPETPKVEKAEVVLPAKVEPPKPKPKKKKQKKAPRTTAPQSSQAQRADRAAAPAEGMASSMSPATWRAALMAHLNRHKRFPPGAGMGVASVAFTIDRSGRVLSARLVRSAGDSSLDAEAVSLPRRASPVPAPPSNVGGGSITLAVPIRFNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUS6</accession>
    <name>Q3SUS6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphatidylserine decarboxylase</fullName>
            <ecNumber evidence="3">4.1.1.65</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Nwi_0699</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12">Phospholipid metabolism; phosphatidylethanolamine biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.65" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0699"/>
    <dbReference type="EnsemblBacteria" id="ABA03965">
        <property type="protein sequence ID" value="ABA03965"/>
        <property type="gene ID" value="Nwi_0699"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0699"/>
    <dbReference type="eggNOG" id="COG0688">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029061_4_0_5"/>
    <dbReference type="OMA" id="AIEWQLH"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00186"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00558"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004609">
        <property type="term" value="F:phosphatidylserine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003817">
        <property type="entry name" value="PS_Dcarbxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033177">
        <property type="entry name" value="PSD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10067">
        <property type="entry name" value="PTHR10067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02666">
        <property type="entry name" value="PS_Dcarbxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00163">
        <property type="entry name" value="PS_decarb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="11" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA03965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="297" mass="33317" checksum="FE5C373A9AB7A70D" modified="2005-10-11" version="1">MRKLISALTEQEDMNFLLTNRIPRAMLTRFMGWFGKIEQPLIRDLSIACWRLFSDLDLSEARKSSFKSLHDCFTRELLPGSRLPDPDPAIVVSPCDAIVGAFGKVADTELFQIKGAPYSLPDLLGDPALVEAHRNGRFITLRLTSSMYHRFHAPHDCRIDRVTFIHGDVWNVNPIALKRVERLFCKNERAVLQARLITGETLTLVPVAAILVASIRLHFIDATLNARTRGPTVFSCDASARKGDELGWFEHGSTIIVLASDEFDFCDNVVESARLKAGQPLMRRVAGDRMAGTQNRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUW1</accession>
    <name>Q3SUW1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Porin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0664</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Forms passive diffusion pores that allow small molecular weight hydrophilic materials across the outer membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell outer membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="4">Consists of 16-stranded beta-barrel sheets, with large surface-exposed loops, that form a transmembrane pore at the center of each barrel. The pore is partially ocluded by a peptide loop that folds into the pore lumen.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the alphaproteobacteria porin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313986.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0664"/>
    <dbReference type="EnsemblBacteria" id="ABA03930">
        <property type="protein sequence ID" value="ABA03930"/>
        <property type="gene ID" value="Nwi_0664"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0664"/>
    <dbReference type="eggNOG" id="COG3203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044836_2_0_5"/>
    <dbReference type="OMA" id="ALHYDDS"/>
    <dbReference type="OrthoDB" id="837523at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003684">
        <property type="entry name" value="Porin_alphabac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02530">
        <property type="entry name" value="Porin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0626">Porin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="2 4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009368702" description="Porin" evidence="4">
        <location>
            <begin position="24"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="512" mass="53908" checksum="BE7B31C8FFE2205C" modified="2005-10-11" version="1">MNLMKNLFLGSTTALVVVSGARAADLPLKAKAVEYVKVCSLYGAGFYYIPGTETCIKLGGYMRADLGVNTNAIFMGNTSNQAGAQNRHSNAFTWRARADLTFDTRTATDYGVVRSYFDGGFTWTSGGYAGLRVGGTAYDGNPTGGISGGQLNVFFAFIQFAGFTMGKAVSQFTAPWGNYPGNIYDGLVGGGGAFTGVNQFTYTAEFGDGISGSIGVQDPSAYYQAGIHNLGVGSPGTIISPPFLGIVFFGSPLFGYGLSANAGTTFPDIVGQLRVDQAWGLFQASAAAHHNNPAYYGGDETTGGPRNKWGWAGQLALTIRNIPTGPGDVINVSAVYTNGATRYNIQDMAAQAGGSTIYSGFGVGSVGFGFAPDSVYAPNGDLELVTTWGMRGAYVHNWNQYWSSSIFGAYASVRYTGGAKAYMCGVSIPGALGGGLSTCNPDYNIGQVGANIRWTPVKNLTFTTEAAYSMLDQKFAGVAAVPPNWAVSKPGGNYAVKDQEVWTVMFRAQRAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUX3</accession>
    <name>Q3SUX3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0652</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0652"/>
    <dbReference type="EnsemblBacteria" id="ABA03918">
        <property type="protein sequence ID" value="ABA03918"/>
        <property type="gene ID" value="Nwi_0652"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0652"/>
    <dbReference type="HOGENOM" id="CLU_180002_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="101" mass="11854" checksum="7B4CAA43E6E85509" modified="2005-10-11" version="1">MSADLSSSFGWIEYLFRSKGGSMSKRVTKTKSCRDREKLGVLRVKDHRKIEERILRLLDRYEASPETDKRWLDIGRSCIEQGFMAINRAVFEQKRAELPED</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV03</accession>
    <name>Q3SV03_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain dehydrogenase/reductase SDR</fullName>
            <ecNumber evidence="1">1.1.1.184</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0621</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.184" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313948.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0621"/>
    <dbReference type="EnsemblBacteria" id="ABA03888">
        <property type="protein sequence ID" value="ABA03888"/>
        <property type="gene ID" value="Nwi_0621"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0621"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_5"/>
    <dbReference type="OMA" id="MFPFGRI"/>
    <dbReference type="OrthoDB" id="1601931at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004090">
        <property type="term" value="F:carbonyl reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="263" mass="28222" checksum="FE8A5AC76756AC43" modified="2005-10-11" version="1">MTPDKAKPRTEMATVADPHAPRRTLLLTGASRGIGHATVIRFSSAGWRVITCSRHPFPEQCPWDAGPEDHIQVDLSDPDNTMEAIQEIRSRLEGGKLHALVNNAAISPKGEGGSRLGSLNTDMETWEKVFRVNFMAPIMLARGLIGELKAARGSIVNVTSIAGSRVHPFAGAAYATSKAALASLTREMAADFGRIGVRVNAIAPGEIDTSILSPGTEKIVQRQIPLRRLGTPDEVSKIVYVLCTETSSYVNGAEIHINGGQHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV80</accession>
    <name>Q3SV80_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">dTDP-glucose 4,6-dehydratase</fullName>
            <ecNumber evidence="4 5">4.2.1.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0544</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>dTDP-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-glucose + H2O</text>
            <dbReference type="Rhea" id="RHEA:17221"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57477"/>
            <dbReference type="ChEBI" id="CHEBI:57649"/>
            <dbReference type="EC" id="4.2.1.46"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 5">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the NAD(P)-dependent epimerase/dehydratase family. dTDP-glucose dehydratase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.46" evidence="4 5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313872.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0544"/>
    <dbReference type="EnsemblBacteria" id="ABA03811">
        <property type="protein sequence ID" value="ABA03811"/>
        <property type="gene ID" value="Nwi_0544"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0544"/>
    <dbReference type="eggNOG" id="COG1088">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_14_5"/>
    <dbReference type="OMA" id="KLIPLMC"/>
    <dbReference type="OrthoDB" id="707977at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008460">
        <property type="term" value="F:dTDP-glucose 4,6-dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009225">
        <property type="term" value="P:nucleotide-sugar metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05246">
        <property type="entry name" value="dTDP_GD_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005888">
        <property type="entry name" value="dTDP_Gluc_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01181">
        <property type="entry name" value="dTDP_gluc_dehyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="6">
        <location>
            <begin position="9"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="355" mass="40279" checksum="7CA688F3F588928C" modified="2005-10-11" version="1">MRFKASTIFVTGGAGFIGSAVVRHLLHDTHARVVNIDKCTYAANLDSLPGTNENLNYAFEKQCICDSAGLRHLFEKYRPDAVMNLAAESHVDRSIDGPGEFIQTNIVGTFTLLQETLRHWRSLSPEKREKFRFLHVSTDEVFGSLGDSGYFTEATAYAPNSPYSASKASSDHLVRAWRETYELPTLITNCSNNYGPYHFPEKLIPHMIIKGLACEPLPVYGDGRNIRDWLYVDDHARALSLVLEHGAVGHTYNVGGRNERTNLHVVESICDLLDEIAPNTTNRHRDLITFVTDRPGHDRRYAIDASKLERELGWRAKEDFESGIEKTVRWYVDEQPWWQAILARGYAARRVGLRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV97</accession>
    <name>Q3SV97_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flagellar FliJ protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_0527</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FliJ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313855.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0527"/>
    <dbReference type="EnsemblBacteria" id="ABA03794">
        <property type="protein sequence ID" value="ABA03794"/>
        <property type="gene ID" value="Nwi_0527"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0527"/>
    <dbReference type="eggNOG" id="ENOG50323UI">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158714_0_0_5"/>
    <dbReference type="OMA" id="QMMMSEF"/>
    <dbReference type="OrthoDB" id="1880233at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009288">
        <property type="term" value="C:bacterial-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044781">
        <property type="term" value="P:bacterial-type flagellum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012823">
        <property type="entry name" value="Flagell_FliJ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02050">
        <property type="entry name" value="FliJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02473">
        <property type="entry name" value="flagell_FliJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="15"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA03794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="141" mass="16428" checksum="3DC2BA1A57BC5DBA" modified="2005-10-11" version="1">MKSRETLIRLKKFQVEEKRRRVAQIESMIADFQRMSVDLEREIEIEQERAGIDDPTHFAYPTYAKAAIQRRENLTRSADELRVQLEEAKMSLAEAFEDLKKVELLDERDQARERAEENAREQADLDSIGLMRSRMGAASIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVA3</accession>
    <name>Q3SVA3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CheW protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0521</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313849.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0521"/>
    <dbReference type="EnsemblBacteria" id="ABA03788">
        <property type="protein sequence ID" value="ABA03788"/>
        <property type="gene ID" value="Nwi_0521"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0521"/>
    <dbReference type="eggNOG" id="COG0835">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048995_3_3_5"/>
    <dbReference type="OMA" id="DERLAMS"/>
    <dbReference type="OrthoDB" id="1711729at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039315">
        <property type="entry name" value="CheW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036061">
        <property type="entry name" value="CheW-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002545">
        <property type="entry name" value="CheW-lke_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22617">
        <property type="entry name" value="PTHR22617"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01584">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00260">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50341">
        <property type="entry name" value="SSF50341"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50851">
        <property type="entry name" value="CHEW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CheW-like" evidence="1">
        <location>
            <begin position="12"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="156" mass="16955" checksum="96834E2C9FDDAC3A" modified="2005-10-11" version="1">MTTVNQANESALIEYVTATIGGQLFGLPISRVQDVFKPERLTRVPLASNDIAGVLNLRGRIVTAIDMRARLKLPKGEADRRPMAVGVELRGESYGLLIDSIGEVMKLPDEGREVNPANLDRCTAELSAGVYRLDGQLMVVLDVDRVLEIAPDKLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVB1</accession>
    <name>Q3SVB1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Acetylornithine aminotransferase</fullName>
            <shortName evidence="2">ACOAT</shortName>
            <ecNumber evidence="2">2.6.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">argD</name>
        <name evidence="4" type="ordered locus">Nwi_0513</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2-oxoglutarate + N(2)-acetyl-L-ornithine = L-glutamate + N-acetyl-L-glutamate 5-semialdehyde</text>
            <dbReference type="Rhea" id="RHEA:18049"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29123"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
            <dbReference type="EC" id="2.6.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
        <text evidence="2">Binds 1 pyridoxal phosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 4/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="2">May also have succinyldiaminopimelate aminotransferase activity, thus carrying out the corresponding step in lysine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. ArgD subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.11" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0513"/>
    <dbReference type="EnsemblBacteria" id="ABA03780">
        <property type="protein sequence ID" value="ABA03780"/>
        <property type="gene ID" value="Nwi_0513"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0513"/>
    <dbReference type="eggNOG" id="COG4992">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_10_1_5"/>
    <dbReference type="OMA" id="PFMVPTY"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00109"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003992">
        <property type="term" value="F:N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01107">
        <property type="entry name" value="ArgD_aminotrans_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004636">
        <property type="entry name" value="AcOrn/SuccOrn_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00707">
        <property type="entry name" value="argD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="2" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="2">
        <location>
            <begin position="120"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="2">
        <location>
            <begin position="238"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>N(2)-acetyl-L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>N(2)-acetyl-L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="423" mass="44833" checksum="AC43CC43A9FD6249" modified="2005-10-11" version="1">MIAPQRGRAEPLPPSNRQTVMTTAAASHLLPVFARADLSFESGEGAWLLGSDGGRYLDFTSGVAVNALGHCHPHLVAALQAQTAKLWHVSNLFKSPDGERLAVRLCEQSFADFVFFANSGAEAVECAIKIVRRYHAGRGNPERYRIVTFEGAFHGRTLAMLAATGSKKYLEGYGPPADGFDQVPLDDIEAVKKAVGPETAGILIEPLQGEGGVREPPPAFFRALRQLCDERGLLLVFDEVQTGMGRTGDLFAYRRIGVAPDVMSLAKALGGGFPIGACLATAEAASGMTPGSHGSTFGGNPLAVAAAHAVLDVMLAPGFFERTRKMSLLLKQKLASLVDRYPDVLSEVRGEGLLIGLKAVVPSGDLVSALREEKLLTVGAGENVVRLLPPLIIGEAEINEAAQRLERACVRLSSASASKEAAQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-05-25" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVC8</accession>
    <name>MTGA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Biosynthetic peptidoglycan transglycosylase</fullName>
            <ecNumber evidence="1">2.4.1.129</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glycan polymerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Peptidoglycan glycosyltransferase MtgA</fullName>
            <shortName evidence="1">PGT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mtgA</name>
        <name type="ordered locus">Nwi_0496</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Peptidoglycan polymerase that catalyzes glycan chain elongation from lipid-linked precursors.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = [GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23708"/>
            <dbReference type="Rhea" id="RHEA-COMP:9602"/>
            <dbReference type="Rhea" id="RHEA-COMP:9603"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:60033"/>
            <dbReference type="ChEBI" id="CHEBI:78435"/>
            <dbReference type="EC" id="2.4.1.129"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 51 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.129" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313824.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVC8"/>
    <dbReference type="SMR" id="Q3SVC8"/>
    <dbReference type="STRING" id="323098.Nwi_0496"/>
    <dbReference type="CAZy" id="GT51">
        <property type="family name" value="Glycosyltransferase Family 51"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03763">
        <property type="protein sequence ID" value="ABA03763"/>
        <property type="gene ID" value="Nwi_0496"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0496"/>
    <dbReference type="eggNOG" id="COG0744">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006354_1_1_5"/>
    <dbReference type="OMA" id="PAPKCFD"/>
    <dbReference type="OrthoDB" id="1793788at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009274">
        <property type="term" value="C:peptidoglycan-based cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008955">
        <property type="term" value="F:peptidoglycan glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3810.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00766">
        <property type="entry name" value="PGT_MtgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001264">
        <property type="entry name" value="Glyco_trans_51"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036950">
        <property type="entry name" value="PBP_transglycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011812">
        <property type="entry name" value="Pep_trsgly"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30400">
        <property type="entry name" value="PTHR30400"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00912">
        <property type="entry name" value="Transgly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02070">
        <property type="entry name" value="mono_pep_trsgly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0133">Cell shape</keyword>
    <keyword id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000257677" description="Biosynthetic peptidoglycan transglycosylase">
        <location>
            <begin position="1"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00766"/>
        </source>
    </evidence>
    <sequence length="226" mass="24936" checksum="5E013CAD37BF8DF2" modified="2005-10-11" version="1">MRRAIRVMALSAIGLLLLPYLLTPLYRIGHPVSTLMIWRTLSGAPMSRQWIDFAALPPSLPRSVVASEDAKFCSHQGIDWDSLREVLDDAEDGEFKRGGSTITQQVAKNLFLWPGRSMVRKALEFPLAMWIDAVLSKQRILEIYLNIAEWGPGGQFGVEAGSRYAFGRSAASLTAREAALMAAILPNPVRRSARKPGPGVRRLAGTYMARARAAELRGCWSGNRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVK9</accession>
    <name>Q3SVK9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter, ATPase subunit</fullName>
            <ecNumber evidence="7">3.6.3.25</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0415</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in beta-(1--&gt;2)glucan export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.25" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313746.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0415"/>
    <dbReference type="EnsemblBacteria" id="ABA03682">
        <property type="protein sequence ID" value="ABA03682"/>
        <property type="gene ID" value="Nwi_0415"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0415"/>
    <dbReference type="eggNOG" id="COG4181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_5"/>
    <dbReference type="OMA" id="FARAIMS"/>
    <dbReference type="OrthoDB" id="1181903at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="20"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="56"/>
            <end position="63"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="240" mass="25188" checksum="7793BF6F55CD8045" modified="2005-10-11" version="1">MDSLIKRSSPASYSEIPDAIAISDVNLTLGSGAARVHILKDISLRIAAAEAIGLIGPSGSGKSTLLMVMAGLERPDSGEVVVNGTSFNALDEDGLARFRGRHVGIVFQSFHLIPTMTALENVAVPLELAGVADASQRAASELEAVGLGARLHHYPTQLSGGEQQRVALARAFAPDPAILVADEPTGNLDEATGQQIVDLLFAKHAERGMTLVLVTHDTALAQRCDRVVRLRSGRIDGAGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVP5</accession>
    <name>Q3SVP5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 15">Aspartokinase</fullName>
            <ecNumber evidence="6 15">2.7.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">Nwi_0379</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>ATP + L-aspartate = 4-phospho-L-aspartate + ADP</text>
            <dbReference type="Rhea" id="RHEA:23776"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 16">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 16">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 16">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 15">Belongs to the aspartokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.4" evidence="6 15"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313710.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0379"/>
    <dbReference type="EnsemblBacteria" id="ABA03646">
        <property type="protein sequence ID" value="ABA03646"/>
        <property type="gene ID" value="Nwi_0379"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0379"/>
    <dbReference type="eggNOG" id="COG0527">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009116_3_2_5"/>
    <dbReference type="OMA" id="DMIVQTI"/>
    <dbReference type="OrthoDB" id="1067792at2"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00015"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00461"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00462"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004072">
        <property type="term" value="F:aspartate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04261">
        <property type="entry name" value="AAK_AKii-LysC-BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041740">
        <property type="entry name" value="AKii-LysC-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005260">
        <property type="entry name" value="Asp_kin_monofn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001341">
        <property type="entry name" value="Asp_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018042">
        <property type="entry name" value="Aspartate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027795">
        <property type="entry name" value="CASTOR_ACT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13840">
        <property type="entry name" value="ACT_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000726">
        <property type="entry name" value="Asp_kin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00656">
        <property type="entry name" value="asp_kin_monofn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00657">
        <property type="entry name" value="asp_kinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00324">
        <property type="entry name" value="ASPARTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 15 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="10 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 15 18" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACT" evidence="17">
        <location>
            <begin position="275"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="7"/>
            <end position="10"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="175"/>
            <end position="176"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="211"/>
            <end position="212"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00016273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000726-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ABA03646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="417" mass="44826" checksum="F59233A0A019A82B" modified="2005-10-11" version="1">MSRLVMKFGGTSVANLDRIRNVARHVKREVDAGHDVAVVVSAMAGKTNELVAWCRDASSMHDAREYDAVVASGEQITSGLLAIALQALGIQARSWQGWQIPIRTSDAHASARILEIDGSELISRFTERKEVAVIAGFQGISPQTGRITTLGRGGSDTSAVAIAAAIRADRCDIYTDVDGVYTTDPRVVSKARRLDRIAFEDMLELASQGAKVLQVRSVELGMTQNMPIFVRSSFDAPEEIDPHGTPPGTLICGEEDIMESHVVTGIAFSKDEAQISVRQIEDKPGVAASIFVPLADANINVDMIVQNVSEDGKTTDLTFTVPASDHARAKETITRAKDMIGYARLDSATDVAKISVIGSGMRSHAGVAAQAFKALSDKGINIRAITTSEIKFSLLIDTAYTELAVRTLHTLYGLDQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVS5</accession>
    <name>Q3SVS5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pyridoxamine 5'-phosphate oxidase-related, FMN-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0349</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313681.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0349"/>
    <dbReference type="EnsemblBacteria" id="ABA03616">
        <property type="protein sequence ID" value="ABA03616"/>
        <property type="gene ID" value="Nwi_0349"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0349"/>
    <dbReference type="eggNOG" id="COG0748">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053419_1_2_5"/>
    <dbReference type="OMA" id="KAHDFDF"/>
    <dbReference type="OrthoDB" id="1822051at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019595">
        <property type="entry name" value="DUF2470"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037119">
        <property type="entry name" value="Haem_oxidase_HugZ-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10615">
        <property type="entry name" value="DUF2470"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2470" evidence="1">
        <location>
            <begin position="167"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="245" mass="26688" checksum="00412CCAE7402B57" modified="2005-10-11" version="1">MQPTANFTAANLTRSLLRRSRQGALATLMAGSGDPYCSLVNVASHYDGSPILLISRLAVHTKNLLADPRVSLMLDERAAGDPLEGSRIMVAGTAEEADGELKAVLRRRYLNVHPSAEGFADFNDFSFFRIRLSGVHLVAGFGRIVDLKPEKVLTSLDGAEALVAAEQNAIEHMNRDHREALNLYATRLLGAAAADWRCTGCDPDGLDMSADRHDALRLDFPRRVATPDELRVILKDLAEKARAAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVU2</accession>
    <name>Q3SVU2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter, ATPase subunit</fullName>
            <ecNumber evidence="7">3.6.3.25</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0332</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in beta-(1--&gt;2)glucan export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.25" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313664.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0332"/>
    <dbReference type="EnsemblBacteria" id="ABA03599">
        <property type="protein sequence ID" value="ABA03599"/>
        <property type="gene ID" value="Nwi_0332"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0332"/>
    <dbReference type="eggNOG" id="COG1116">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_5"/>
    <dbReference type="OMA" id="FQLHDGV"/>
    <dbReference type="OrthoDB" id="1832232at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="21"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="54"/>
            <end position="61"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="267" mass="29030" checksum="A25BF2DC1A4E7694" modified="2005-10-11" version="1">MIDHAIASYPSGVGPRAGDAVSLKGVTKRYDRGVVALGPVDFNVRKGDFVSLLGPSGCGKSTALRIIAGLSEPTSGRVQLAGGGRSIGFVFQEPTLMPWATVRDNVALPLKLSRPRRADIGDRVEVALARVGLAEFAAAYPRQLSGGMKMRVSLARALVTDPDVLLMDEPFAALDEITRFHLNNDLLALWRGLRKTVIFVTHSVFESVYLSRRVVVMTPRPGRICAEYAIDTAEPRREDFRTSAEYAAYCREVSSALSRAVAGRARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVV4</accession>
    <name>Q3SVV4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Heme exporter protein C</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Cytochrome c-type biogenesis protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ccmC</name>
        <name evidence="10" type="ordered locus">Nwi_0320</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Required for the export of heme to the periplasm for the biogenesis of c-type cytochromes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the CcmC/CycZ/HelC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313652.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0320"/>
    <dbReference type="EnsemblBacteria" id="ABA03587">
        <property type="protein sequence ID" value="ABA03587"/>
        <property type="gene ID" value="Nwi_0320"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0320"/>
    <dbReference type="eggNOG" id="COG0755">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066538_2_1_5"/>
    <dbReference type="OMA" id="FHVPMWF"/>
    <dbReference type="OrthoDB" id="955123at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015232">
        <property type="term" value="F:heme transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002541">
        <property type="entry name" value="Cyt_c_assembly"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003557">
        <property type="entry name" value="Cyt_c_biogenesis_CcmC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045062">
        <property type="entry name" value="Cyt_c_biogenesis_CcsA/CcmC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30071">
        <property type="entry name" value="PTHR30071"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01578">
        <property type="entry name" value="Cytochrom_C_asm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01386">
        <property type="entry name" value="CCMCBIOGNSIS"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01191">
        <property type="entry name" value="ccmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="197"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrom_C_asm" evidence="9">
        <location>
            <begin position="23"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA03587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="243" mass="26756" checksum="8AD3DADB0A52B9E0" modified="2005-10-11" version="1">MSLIDLANPSRFLTLAGRILPWLVAVTALVLAVGLYQSAIAPDDYQQGATVKIMFIHVPNAWLSMFVWGVMSVAALGTLVWRHPLADVAAKTAAPIGAAFTFLALLTGSLWGRPMWGTYWEWDARLTSVLILFLMYLGLMALWRAVEDPSRAARAAAVLTLVGSVNIPIIKFSVDWWNTLHQPASVVRMGGSTLDRAFLIPLVVMAIGFTLLFLTLHIAAMRNEILRRRVRTLQMLQASKSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVZ5</accession>
    <name>Q3SVZ5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Thiolase</fullName>
            <ecNumber evidence="6">2.3.1.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_0279</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.16" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313612.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0279"/>
    <dbReference type="EnsemblBacteria" id="ABA03546">
        <property type="protein sequence ID" value="ABA03546"/>
        <property type="gene ID" value="Nwi_0279"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0279"/>
    <dbReference type="eggNOG" id="COG0183">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031026_1_1_5"/>
    <dbReference type="OMA" id="EWNVDLP"/>
    <dbReference type="OrthoDB" id="330994at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003988">
        <property type="term" value="F:acetyl-CoA C-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020610">
        <property type="entry name" value="Thiolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00099">
        <property type="entry name" value="THIOLASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase_N" evidence="4">
        <location>
            <begin position="5"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase_C" evidence="5">
        <location>
            <begin position="272"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="2">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="350"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA03546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="395" mass="41190" checksum="F3CD213B09DA0E4B" modified="2005-10-11" version="1">MTEAVIVSTARTPIGKAYRGALNATEGATLLGHAIGEAAVRAGVDPQEIEDVVMGVAMQQGATGGNIARKALLRAGLPVTVAGATIDRQCASGLQAIALAARSVLFDGVGIAVGGGGESISLVQNEHANTFHAEDPALLEIKREVYMPMIDTAEVVAKRYGISRQAQDEYALESQRRTAAAQQEGRFKDELAPISTRMAIVDKETGNVTFRDVTLSQDEGPRPDTSAEGLAGLKPVRGEGCTITAGNASQLSDGASAAVIMSGTEASRRGLNPLGIFRGFVSAGCEPDEMGIGPVFAVPRLLKRHGLTVGDIDLWELNEAFAVQVIYCRDRLGIDPEKLNVDGGAIAVGHPYGMSGARLAGHALIEGRRRKAKYAVVTMCVGGGMGAAGLFEVLQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW37</accession>
    <name>ATPF2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName>ATP synthase subunit b 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>ATP synthase F(0) sector subunit b 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>ATPase subunit I 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>F-type ATPase subunit b 2</fullName>
            <shortName>F-ATPase subunit b 2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">atpF2</name>
        <name type="synonym">atpG</name>
        <name type="ordered locus">Nwi_0236</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Component of the F(0) channel, it forms part of the peripheral stalk, linking F(1) to F(0). The b'-subunit is a diverged and duplicated form of b found in plants and photosynthetic bacteria.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">F-type ATPases have 2 components, F(1) - the catalytic core - and F(0) - the membrane proton channel. F(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). F(0) has three main subunits: a(1), b(2) and c(10-14). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. F(1) is attached to F(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ATPase B chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313571.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SW37"/>
    <dbReference type="SMR" id="Q3SW37"/>
    <dbReference type="STRING" id="323098.Nwi_0236"/>
    <dbReference type="EnsemblBacteria" id="ABA03504">
        <property type="protein sequence ID" value="ABA03504"/>
        <property type="gene ID" value="Nwi_0236"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0236"/>
    <dbReference type="eggNOG" id="COG0711">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079215_1_2_5"/>
    <dbReference type="OMA" id="NQIFWLV"/>
    <dbReference type="OrthoDB" id="1544382at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045263">
        <property type="term" value="C:proton-transporting ATP synthase complex, coupling factor F(o)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01398">
        <property type="entry name" value="ATP_synth_b_bprime"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002146">
        <property type="entry name" value="ATP_synth_b/b'su_bac/chlpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00430">
        <property type="entry name" value="ATP-synt_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0138">CF(0)</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000369017" description="ATP synthase subunit b 2">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="185" mass="19483" checksum="A55EF25C650CC886" modified="2005-10-11" version="1">MAESHGNAHGATAHTEADGGHKAPFPPFQKETFASQLVSLTIAFVALYLISSRLALPRVRQTIDDRENTIKGDLAQAQKLKDDSDAALKAYEAELAAARARAQAIGNETREKLNAAAEAERKALEERLSVKLADAEKTIASTRAAAMSNVRGIASDAATAIVQQLTGATPDSKLVDSAVDASMKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW43</accession>
    <name>Q3SW43_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Peptidase M16</fullName>
            <ecNumber evidence="6">3.4.24.64</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_0230</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the peptidase M16 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.64" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313565.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0230"/>
    <dbReference type="EnsemblBacteria" id="ABA03498">
        <property type="protein sequence ID" value="ABA03498"/>
        <property type="gene ID" value="Nwi_0230"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0230"/>
    <dbReference type="eggNOG" id="COG0612">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009902_3_0_5"/>
    <dbReference type="OMA" id="IDVVCDM"/>
    <dbReference type="OrthoDB" id="1188251at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="SSF63411"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M16" evidence="4">
        <location>
            <begin position="14"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M16_C" evidence="5">
        <location>
            <begin position="167"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA03498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="429" mass="45848" checksum="FCA4F71D555BBB5B" modified="2005-10-11" version="1">MSVNVTKLSSGLTVVTDDMPHLETAALGVWTGVGGRDEKPDEHGISHLLEHMAFKGTTTRSSRAIAEEIEAVGGDLNAATGVETTAYYARVMKADVPLALDVLSDILSNPIFDAKELECEKGVIEQEIGAAQDTPDDVVFEQLNELCYPDQPIGRSLLGTPETLERFNSDMLHGYLKTHYRGPDMVVAAAGAIEHGMVVAEVERRFAGFDAAPPPQPAAATFGHGGSRVVHRDLEQAHLTLALEGVPQTDGSLFSLQVFTSVLGGGMSSRLFQEVREKRGLCYSIYTFHAPYADTGLFGLYTGTDPSDAPEMMEVIVDVISEAVETLTEAEIARAKAQMKAGLLMALESCSARAEQLARHMLVYGRPQSVGELTARIDAVSVESTRDAARGLLARSRPAVVALGSGRGLDSAVAFAEGLAQSKARILLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW56</accession>
    <name>Q3SW56_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Probable lipid II flippase MurJ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">murJ</name>
        <name evidence="10" type="ordered locus">Nwi_0217</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Involved in peptidoglycan biosynthesis. Transports lipid-linked peptidoglycan precursors from the inner to the outer leaflet of the cytoplasmic membrane.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell inner membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the MurJ/MviN family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0217"/>
    <dbReference type="EnsemblBacteria" id="ABA03485">
        <property type="protein sequence ID" value="ABA03485"/>
        <property type="gene ID" value="Nwi_0217"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0217"/>
    <dbReference type="eggNOG" id="COG0728">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006797_5_0_5"/>
    <dbReference type="OMA" id="WLYFANR"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015648">
        <property type="term" value="F:lipid-linked peptidoglycan transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13123">
        <property type="entry name" value="MATE_MurJ_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02078">
        <property type="entry name" value="MurJ_MviN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004268">
        <property type="entry name" value="MurJ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03023">
        <property type="entry name" value="MurJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002869">
        <property type="entry name" value="MviN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01806">
        <property type="entry name" value="VIRFACTRMVIN"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01695">
        <property type="entry name" value="murJ_mviN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2 8 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 8 9" id="KW-0133">Cell shape</keyword>
    <keyword evidence="8 9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7 8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 8 9" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="89"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="198"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="322"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="361"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="403"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="461"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="487"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR002869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA03485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="533" mass="54999" checksum="21933904CBEE9116" modified="2005-10-11" version="1">MALPCHQARYAGLAMIRSILTVSSGTLASRLLGFGRDALTAALLGAGPVADAFLMAFQFINVIRRMLGEGALNVALVPAWMRLREVSGLAAASAFAGDVLATVSATLIALAAVAGVAMPLLVGMLAPGFVGRESLQLAVTDARLMLPYLAFAGPAAVIMSLLNARHRFAIAAFSPLLFNVALILAAALLLWLQPDSQFAALVMAATVGIAGLLQLAVLALLTRRDDLASPLRISFGPGMRDFARKAVPGMIANSGPQLLIVAGAVVASMSPSGVSWLYFANRLIELPLGMVGVAIGTVLVPEMSRALSKDDRTAAVHAESRGIELAAALALPATLGLIVLSEPIVRVLFEHGAFTTADTRATALALGCLALGLPAHILIKALSPAFFARGDTMMPLIATLKGIAVAIVSAVALGWMVGVCGVALAMALGAWSSAASLARSMAAGFGFSIDTEARRRLPRITLAALVMGGVLWLALRLLAPISGAHGLLQAATLIILIAAGVAVYGLLLLTFRVIDRNDIQTALARSHSGDLRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW61</accession>
    <name>Q3SW61_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0212</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313547.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0212"/>
    <dbReference type="EnsemblBacteria" id="ABA03480">
        <property type="protein sequence ID" value="ABA03480"/>
        <property type="gene ID" value="Nwi_0212"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0212"/>
    <dbReference type="eggNOG" id="COG5568">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_181511_0_0_5"/>
    <dbReference type="OMA" id="GEHKQNL"/>
    <dbReference type="OrthoDB" id="2089322at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009531">
        <property type="entry name" value="DUF1150"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06620">
        <property type="entry name" value="DUF1150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="88" mass="9428" checksum="28F446639A8A6EAC" modified="2005-10-11" version="1">MSEVISTIRPESVSTESLAQLGEGHIAYVKQVRSEDVPELFPQAPRIAPGLMLFALHAADGTPIMLTDSREAAVASAWSNELQAVSVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW68</accession>
    <name>Q3SW68_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Endonuclease III</fullName>
            <ecNumber evidence="2">4.2.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">DNA-(apurinic or apyrimidinic site) lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">nth</name>
        <name evidence="5" type="ordered locus">Nwi_0205</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-monophospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="2">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the Nth/MutY family.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313541.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0205"/>
    <dbReference type="EnsemblBacteria" id="ABA03473">
        <property type="protein sequence ID" value="ABA03473"/>
        <property type="gene ID" value="Nwi_0205"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0205"/>
    <dbReference type="eggNOG" id="COG0177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012862_3_0_5"/>
    <dbReference type="OMA" id="QIIWYGR"/>
    <dbReference type="OrthoDB" id="1381897at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019104">
        <property type="term" value="F:DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00942">
        <property type="entry name" value="Nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004035">
        <property type="entry name" value="Endouclease-III_FeS-bd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005759">
        <property type="entry name" value="Nth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01083">
        <property type="entry name" value="nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00764">
        <property type="entry name" value="ENDONUCLEASE_III_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="2" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2 5" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENDO3c" evidence="4">
        <location>
            <begin position="76"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="252" mass="27659" checksum="92A941DCC4AFE3FC" modified="2005-10-11" version="1">MKKRTGKPPARQAEPVSGVRRAVKPARGPAELPPSLRPWTPAEVHEAFTRFRRANPEPKGELEHLNPFTLLVAVVLSAQATDAGVNKATRALFAVADTPARMLALGEEKVRDYIKTIGLYRTKARNIIALSEKLLAEFDGAVPPSRAGIESLPGAGRKTANVVLNMAFGERTMAVDTHVFRVANRTGMAPGKTPLEVELGLERVIPNQFMLHAHHWLILHGRYTCLARSPRCKVCLINDLCRWPEKTTEGAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW81</accession>
    <name>Q3SW81_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">dITP/XTP pyrophosphatase</fullName>
            <ecNumber evidence="7">3.6.1.66</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Non-canonical purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Non-standard purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Nucleoside-triphosphate diphosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Nucleoside-triphosphate pyrophosphatase</fullName>
            <shortName evidence="7">NTPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_0192</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>H2O + ITP = diphosphate + H(+) + IMP</text>
            <dbReference type="Rhea" id="RHEA:29399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>H2O + XTP = diphosphate + H(+) + XMP</text>
            <dbReference type="Rhea" id="RHEA:28610"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:61314"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>dITP + H2O = dIMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28342"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61194"/>
            <dbReference type="ChEBI" id="CHEBI:61382"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="7">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the HAM1 NTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.66" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313528.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0192"/>
    <dbReference type="EnsemblBacteria" id="ABA03460">
        <property type="protein sequence ID" value="ABA03460"/>
        <property type="gene ID" value="Nwi_0192"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0192"/>
    <dbReference type="eggNOG" id="COG0127">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082080_0_0_5"/>
    <dbReference type="OMA" id="DCFADDT"/>
    <dbReference type="OrthoDB" id="1824064at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035870">
        <property type="term" value="F:dITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036220">
        <property type="term" value="F:ITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:nucleoside-triphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036222">
        <property type="term" value="F:XTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009146">
        <property type="term" value="P:purine nucleoside triphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00515">
        <property type="entry name" value="HAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01405">
        <property type="entry name" value="Non_canon_purine_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020922">
        <property type="entry name" value="dITP/XTP_pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002637">
        <property type="entry name" value="Ham1p-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067">
        <property type="entry name" value="PTHR11067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01725">
        <property type="entry name" value="Ham1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="SSF52972"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00042">
        <property type="entry name" value="TIGR00042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="13"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="160"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="195"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="74"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA03460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="211" mass="22466" checksum="23D0D1A90534A3FC" modified="2005-10-11" version="1">MHRRIAGKLVIATHNPGKLAEIRELLAPYGVEAVSAGELGLDEPEETGEDFRANARIKATSAALSAKLPAFADDSGLVVDALDGAPGIHSARWAGKPADFSAAMARIERLLQERGATSADQRKAHFISALCVAWPDGHLQEVEGRVDGTLVWPPRGTAGFGYDPVFLPDGHGRTFGEMAGIEKHGLPPRGLGLSHRARAFVKLAEIALEPR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW84</accession>
    <name>HTPX_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protease HtpX homolog</fullName>
            <ecNumber evidence="1">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">htpX</name>
        <name type="ordered locus">Nwi_0189</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M48B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313525.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SW84"/>
    <dbReference type="STRING" id="323098.Nwi_0189"/>
    <dbReference type="EnsemblBacteria" id="ABA03457">
        <property type="protein sequence ID" value="ABA03457"/>
        <property type="gene ID" value="Nwi_0189"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0189"/>
    <dbReference type="eggNOG" id="COG0501">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042266_3_0_5"/>
    <dbReference type="OMA" id="AVCCTEG"/>
    <dbReference type="OrthoDB" id="1918093at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00188">
        <property type="entry name" value="Pept_M48_protease_HtpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022919">
        <property type="entry name" value="Pept_M48_protease_HtpX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0482">Metalloprotease</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_1000020902" description="Protease HtpX homolog">
        <location>
            <begin position="1"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="277"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="307" mass="32379" checksum="0D39A94EE475236A" modified="2005-10-11" version="1">MNYFRTAILLAGLTGLFMGVGYLIGGASGATIALVVAAATNLFAYWNSDRMVLSMYGAHEVDPGTAPDLHRLVAELASRAGLPMPRVFVMDNPQPNAFATGRNPENAAVAVTTGLMQSLSREELAGVIAHELAHIKHHDTLLMTITATIAGAISMLAQFGMFFGGNRDNHGPGIIGSLAMMILAPFGAMLVQMAISRTREYAADEMGARICGQPMWLASALARIENAAHQVPNMEAERAPATAHMFIINPLSGRGMDNLFATHPSTENRIAALQRLAGQSGGGLAPGGPPPDPSSPWNKGSRRGPWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWB8</accession>
    <name>Q3SWB8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0155</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SDHAF4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0155"/>
    <dbReference type="EnsemblBacteria" id="ABA03423">
        <property type="protein sequence ID" value="ABA03423"/>
        <property type="gene ID" value="Nwi_0155"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0155"/>
    <dbReference type="eggNOG" id="COG5508">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_160299_1_1_5"/>
    <dbReference type="OMA" id="PSRYGDW"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012875">
        <property type="entry name" value="SDHF4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07896">
        <property type="entry name" value="DUF1674"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="33"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="88" mass="9713" checksum="172C4193928E3216" modified="2005-10-11" version="1">MEVIFHAIRSSRRSEDDMIDEPSASSRTGETRSRKQLSPEARRALAEAEARRAAQTDASAPPKEIQGPEGPEPTRFGDWERGGIASDF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWG7</accession>
    <name>SECB_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein-export protein SecB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">secB</name>
        <name type="ordered locus">Nwi_0106</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the proteins required for the normal export of preproteins out of the cell cytoplasm. It is a molecular chaperone that binds to a subset of precursor proteins, maintaining them in a translocation-competent state. It also specifically binds to its receptor SecA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer, a dimer of dimers. One homotetramer interacts with 1 SecA dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SecB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313443.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWG7"/>
    <dbReference type="SMR" id="Q3SWG7"/>
    <dbReference type="STRING" id="323098.Nwi_0106"/>
    <dbReference type="EnsemblBacteria" id="ABA03374">
        <property type="protein sequence ID" value="ABA03374"/>
        <property type="gene ID" value="Nwi_0106"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0106"/>
    <dbReference type="eggNOG" id="COG1952">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111574_0_0_5"/>
    <dbReference type="OMA" id="CPNVLFP"/>
    <dbReference type="OrthoDB" id="1624074at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051262">
        <property type="term" value="P:protein tetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00821">
        <property type="entry name" value="SecB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003708">
        <property type="entry name" value="SecB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035958">
        <property type="entry name" value="SecB-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36918">
        <property type="entry name" value="PTHR36918"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02556">
        <property type="entry name" value="SecB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01594">
        <property type="entry name" value="SECBCHAPRONE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54611">
        <property type="entry name" value="SSF54611"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00809">
        <property type="entry name" value="secB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0653">Protein transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0811">Translocation</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000318249" description="Protein-export protein SecB">
        <location>
            <begin position="1"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00821"/>
        </source>
    </evidence>
    <sequence length="159" mass="17453" checksum="ADCF43B22A763486" modified="2005-10-11" version="1">MTNGNGTPPETAPSPQLNVLAQYTKDFSFENPNSPASLAPQQQPPSINVQINVGASNVAENDYEVVLKVEGKAEHAGKLMFSFELAYAGVFRIVNVPQENLHPLVMIECPRLLFPFAREIVATAVRDGGFPPLMLDPIDFVSLYRQNMERQAAAQQQPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWG8</accession>
    <name>Q3SWG8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">DNA polymerase III subunit epsilon</fullName>
            <ecNumber evidence="3 8">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">dnaQ</name>
        <name evidence="10" type="ordered locus">Nwi_0105</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 8">DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The epsilon subunit contain the editing function and is a proofreading 3'-5' exonuclease.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 8">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7 8">DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the POLIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="3 8"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313442.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0105"/>
    <dbReference type="EnsemblBacteria" id="ABA03373">
        <property type="protein sequence ID" value="ABA03373"/>
        <property type="gene ID" value="Nwi_0105"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0105"/>
    <dbReference type="eggNOG" id="COG0847">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047806_2_1_5"/>
    <dbReference type="OMA" id="FHVYLNP"/>
    <dbReference type="OrthoDB" id="1985371at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06131">
        <property type="entry name" value="DNA_pol_III_epsilon_Ecoli_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006054">
        <property type="entry name" value="DnaQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006309">
        <property type="entry name" value="DnaQ_proteo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Exonuclease_RNaseT/DNA_pol3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00573">
        <property type="entry name" value="dnaq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01406">
        <property type="entry name" value="dnaQ_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="8" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8 10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Exonuclease" evidence="9">
        <location>
            <begin position="2"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA03373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="239" mass="27119" checksum="16EB3DF0AB5DD18E" modified="2005-10-11" version="1">MREIVLDTETTGLDPLRGDRLVEIGCVEMFNRMPTGQTFHRYINPERDMPQEAFAVHGLSSDFLADKPLFAHVVDEFLEFIGDAPLVIHNASFDISFINAELDRLKRQAIGRDRLVDTLLLARRKHPGVSNRLDDLCSRYAIDNSRRTKHGALLDAELLAEVYIDLIGARQSQLILVETAPRQRGGSLEMPRRQRTVPLASRVTAADRIAHREFIATLGEKAIWMEFLEQDSEKTRTGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWJ0</accession>
    <name>Q3SWJ0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA translocase FtsK</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">Nwi_0083</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential cell division protein that coordinates cell division and chromosome segregation. The N-terminus is involved in assembly of the cell-division machinery. The C-terminus functions as a DNA motor that moves dsDNA in an ATP-dependent manner towards the dif recombination site, which is located within the replication terminus region. Translocation stops specifically at Xer-dif sites, where FtsK interacts with the Xer recombinase, allowing activation of chromosome unlinking by recombination. FtsK orienting polar sequences (KOPS) guide the direction of DNA translocation. FtsK can remove proteins from DNA as it translocates, but translocation stops specifically at XerCD-dif site, thereby preventing removal of XerC and XerD from dif.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homohexamer. Forms a ring that surrounds DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FtsK/SpoIIIE/SftA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313420.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0083"/>
    <dbReference type="EnsemblBacteria" id="ABA03351">
        <property type="protein sequence ID" value="ABA03351"/>
        <property type="gene ID" value="Nwi_0083"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0083"/>
    <dbReference type="eggNOG" id="COG1674">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001981_6_1_5"/>
    <dbReference type="OMA" id="IGPANHA"/>
    <dbReference type="OrthoDB" id="349533at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025199">
        <property type="entry name" value="FtsK_4TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041027">
        <property type="entry name" value="FtsK_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002543">
        <property type="entry name" value="FtsK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018541">
        <property type="entry name" value="Ftsk_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13491">
        <property type="entry name" value="FtsK_4TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17854">
        <property type="entry name" value="FtsK_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09397">
        <property type="entry name" value="FtsK_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01580">
        <property type="entry name" value="FtsK_SpoIIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00843">
        <property type="entry name" value="Ftsk_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50901">
        <property type="entry name" value="FTSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="79"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="155"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="FtsK" evidence="14">
        <location>
            <begin position="466"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="265"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="286"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="483"/>
            <end position="490"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABA03351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="833" mass="89371" checksum="0227119B2E1A1C4F" modified="2005-10-11" version="1">MSMAAIERLLPIVDQIPPSIREPLARRLRELTGLGLIGLSGAAAAALMTWSVQDPSLSHATSRPIHNVLGYPGAIGADLLMQLLGLGAIMLILPVAIWGWRMLTHRPFDREASRLACWILCTTSAAGFASCWPHNGAWPLPTGLGGVVGDALVRAPAILFGPAGVLQSILLGAIMLLVMAATFLWASGIRSRPAEELPEIEDDAPFDEGDDHASVSLGWAVHALMSAKARLKRLKIGALLALAYKSLVSSAPRNSGALAFERQEPVLGGGPVAPSLAPGRAGHDDDIDDEPDDDPEDEAEDEEDNDAPPVVAAPRRKAAPRQPAKKAGKFELPSVNVLSAPRASDRQPLSKSELEANSRALEGVLGDFGVRGEIVKANPGPVVTLYELEPAPGIKSARVIGLADDIARSMSALSARVAVVPGRNAIGIELPNAHREKVYLRELLTAREATESVAKLPLCLGKTIGGDPVIIDLARTPHMLIAGTTGSGKSVAINTMILSLLYRLRPDQCRLIMVDPKMLELSVYDGIPHLLTPVVTDPKKAVVALKWAVREMEERYKKMAKLGVRNIDGYNTRLVDAKAKGEELTRTVHTGFDKETGKAIYEEEKLEFEPLPFIVIIVDEMADLMMVAGKDIEGAVQRLAQMARAAGLHVILATQRPSVDVITGTIKANFPTRIAFQVTSKIDSRTILGEMGAEQLLGQGDMLYMAGGGRISRVHGPFASDEEVEKVVRHLKTQGAPEYLEAVTAEEPAEGEDGAVFDGTSMGSDGGGDLFAQAVAIVKRDRKASTSYIQRRLQIGYNRAASLMERMELEGIVGQANHAGKREILIEEEEGQF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-26" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWP8</accession>
    <name>RBFA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosome-binding factor A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rbfA</name>
        <name type="ordered locus">Nwi_0025</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Required for efficient processing of 16S rRNA. May interact with the 5'-terminal helix region of 16S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer. Binds 30S ribosomal subunits, but not 50S ribosomal subunits or 70S ribosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RbfA family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="ABA03293" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03293.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_041344619.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWP8"/>
    <dbReference type="SMR" id="Q3SWP8"/>
    <dbReference type="STRING" id="323098.Nwi_0025"/>
    <dbReference type="EnsemblBacteria" id="ABA03293">
        <property type="protein sequence ID" value="ABA03293"/>
        <property type="gene ID" value="Nwi_0025"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0025"/>
    <dbReference type="eggNOG" id="COG0858">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089475_1_0_5"/>
    <dbReference type="OrthoDB" id="1971380at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00003">
        <property type="entry name" value="RbfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000238">
        <property type="entry name" value="RbfA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023799">
        <property type="entry name" value="RbfA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020053">
        <property type="entry name" value="Ribosome-bd_factorA_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33515">
        <property type="entry name" value="PTHR33515"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02033">
        <property type="entry name" value="RBFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89919">
        <property type="entry name" value="SSF89919"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00082">
        <property type="entry name" value="rbfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01319">
        <property type="entry name" value="RBFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="chain" id="PRO_0000321232" description="Ribosome-binding factor A">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="137" mass="15613" checksum="42937B8873677B47" modified="2008-02-26" version="2">MPRRHQPEKHPGGSHRQLRVAETVRHAIADILAQGQVHDPVLEGHLVTVPEVRMSADLKLATIYVMPLGGRDTADVIDALDRNRKFLRGEIARRVNLKFAPDIRFRVDERFDEAERIEKLLRTPAVQRDLAPDTDES</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMU3</accession>
    <name>Q3SMU3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UspA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313347.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0008"/>
    <dbReference type="EnsemblBacteria" id="ABA03276">
        <property type="protein sequence ID" value="ABA03276"/>
        <property type="gene ID" value="Nwi_0008"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0008"/>
    <dbReference type="eggNOG" id="COG0589">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117163_0_0_5"/>
    <dbReference type="OMA" id="ECLNAMR"/>
    <dbReference type="OrthoDB" id="1490146at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Usp" evidence="1">
        <location>
            <begin position="17"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="164" mass="17597" checksum="A3E1C7FD928E25A1" modified="2005-10-11" version="1">MSAQRRSYESGHKPKYLVVVDDSAEWDRAVYYASRRAIRVGGGIVMLRVVEPEDRNQEWLGIAEVIRAEAYEGADAALDRASGRANGIAAITPERIIREGDPLEQILDVIEKDLDISMLVLAASGGPEGPGPIITTLAKTAGNFPIPVTIVPGTLTDDAIDALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMZ2</accession>
    <name>Q3SMZ2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_3102</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316262.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06349">
        <property type="protein sequence ID" value="ABA06349"/>
        <property type="gene ID" value="Nwi_3102"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3102"/>
    <dbReference type="HOGENOM" id="CLU_2001460_0_0_5"/>
    <dbReference type="OrthoDB" id="2082849at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="124" mass="14034" checksum="498DE54507071AEE" modified="2005-10-11" version="1">MEDALGDKVRTFVDFWIQSHIRAEGYQAKGDTRLASALARKCIEMAQTEGISASDIEAEFGDIIEYVHWQIKRVNDDLAMSVRDVDRASIRRIDQQLEEGLIDSFPASDPVSVTQPSKSRYEAR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN22</accession>
    <name>ARGB_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acetylglutamate kinase</fullName>
            <ecNumber evidence="1">2.7.2.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">N-acetyl-L-glutamate 5-phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">NAG kinase</fullName>
            <shortName evidence="1">NAGK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argB</name>
        <name type="ordered locus">Nwi_3071</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent phosphorylation of N-acetyl-L-glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamyl 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:14629"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:57936"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the acetylglutamate kinase family. ArgB subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.8" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316235.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SN22"/>
    <dbReference type="SMR" id="Q3SN22"/>
    <dbReference type="STRING" id="323098.Nwi_3071"/>
    <dbReference type="EnsemblBacteria" id="ABA06319">
        <property type="protein sequence ID" value="ABA06319"/>
        <property type="gene ID" value="Nwi_3071"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3071"/>
    <dbReference type="eggNOG" id="COG0548">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053680_0_0_5"/>
    <dbReference type="OMA" id="EGLYEDW"/>
    <dbReference type="OrthoDB" id="901370at2"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00107"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003991">
        <property type="term" value="F:acetylglutamate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04250">
        <property type="entry name" value="AAK_NAGK-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00082">
        <property type="entry name" value="ArgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004662">
        <property type="entry name" value="AcgluKinase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037528">
        <property type="entry name" value="ArgB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041727">
        <property type="entry name" value="NAGK-C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000728">
        <property type="entry name" value="NAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00761">
        <property type="entry name" value="argB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000264724" description="Acetylglutamate kinase">
        <location>
            <begin position="1"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="69"/>
            <end position="70"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="34"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00082"/>
        </source>
    </evidence>
    <sequence length="298" mass="31386" checksum="823B48783D6017B1" modified="2005-10-11" version="1">MPDDSRISPLDQARILSEALPHMQRYDEETIVIKYGGHAMGSENVARQFASDIVLLEQTAINPVVVHGGGPQIAAMLQRLGIKSEFAAGLRITDAATIEIVEMVLAGSINKQLVGYINEAGGKAVGLCGKDGNMVTAAKATRMLADPDSNIEKVVDLGFVGEPEKVDLTLLNQLIGHELIPVLAPLATSKGGQTFNVNADIFAGAVAGALKAKRLLLLTDVPGVLDKSKKLIPELSISDARKLIADGTISGGMIPKVETCIYALEQGVSGVVIIDGKTPHAVLLELFTNQGTGTLIHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN69</accession>
    <name>Q3SN69_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sulfite dehydrogenase (Cytochrome) subunit SorA apoprotein</fullName>
            <ecNumber evidence="6">1.8.2.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_3022</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mo-molybdopterin</name>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="1.8.2.1" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316190.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3022"/>
    <dbReference type="EnsemblBacteria" id="ABA06272">
        <property type="protein sequence ID" value="ABA06272"/>
        <property type="gene ID" value="Nwi_3022"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3022"/>
    <dbReference type="eggNOG" id="COG2041">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003827_5_5_5"/>
    <dbReference type="OMA" id="SAWQWWE"/>
    <dbReference type="OrthoDB" id="1345710at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050310">
        <property type="term" value="F:sulfite dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005066">
        <property type="entry name" value="MoCF_OxRdtse_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008335">
        <property type="entry name" value="Mopterin_OxRdtase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000572">
        <property type="entry name" value="OxRdtase_Mopterin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036374">
        <property type="entry name" value="OxRdtase_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03404">
        <property type="entry name" value="Mo-co_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00174">
        <property type="entry name" value="Oxidored_molyb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00407">
        <property type="entry name" value="EUMOPTERIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56524">
        <property type="entry name" value="SSF56524"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="SSF81296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidored_molyb" evidence="4">
        <location>
            <begin position="89"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Mo-co_dimer" evidence="5">
        <location>
            <begin position="285"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA06272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="400" mass="43905" checksum="8ED118A335DFEC24" modified="2005-10-11" version="1">MLDRRDMMKRIGAGLALGVGLAPLKVLALDRVTMPFDNGERPLVKYPQKRPMIGITSRPPQLETPFPVFNEGPITPNDAFFVRYHLADIPLDIDPATFTLEIKGKVDHPLRLSLEDIKAMAQTEIVAVNQCSGNSRGFVDPRVAGGQLGHGAMGNARWRGVSLKTVLDRAGVQHGAKQVVFGGMDGPVSDTTPDFAKALDVDHARDGEVMLAHLMNGRDLPFLNGFPLRLVVPGYYGTYWIKHLNEITVIDNTYDNFWMKKAYRIPDNACACVEPGTTPTATVPIGRLNVRSFITNIPEGAQLRRGGKILKGIAFDGGTGIRNVEVSVDGGKTWKGTRLGRDYGRYAFREWKTPVRLAPGPHELKVRATSNGGDVQPAEARWNPAGYMRNVIETVRVTVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN98</accession>
    <name>Q3SN98_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">3-hydroxyacyl-CoA dehydrogenase</fullName>
            <ecNumber evidence="2">1.1.1.35</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_2993</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:22432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57318"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
            <dbReference type="EC" id="1.1.1.35"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.35" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316165.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2993"/>
    <dbReference type="EnsemblBacteria" id="ABA06243">
        <property type="protein sequence ID" value="ABA06243"/>
        <property type="gene ID" value="Nwi_2993"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2993"/>
    <dbReference type="eggNOG" id="COG1024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1250">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_16_2_5"/>
    <dbReference type="OMA" id="GYGWPVY"/>
    <dbReference type="OrthoDB" id="977512at2"/>
    <dbReference type="UniPathway" id="UPA00659"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003857">
        <property type="term" value="F:3-hydroxyacyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006176">
        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006108">
        <property type="entry name" value="3HC_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00725">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02737">
        <property type="entry name" value="3HCDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3HCDH_N" evidence="9">
        <location>
            <begin position="327"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="3HCDH" evidence="8">
        <location>
            <begin position="502"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA06243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="696" mass="75292" checksum="10A01993724253AD" modified="2005-10-11" version="1">MNSKVLDVLQDRVLELGPKPGAGGPYRHFKLTRDSDGVAWVLFDRKDSSANTLSAEVMEEFAAILTVLENQTPAGVVIRSAKTSGFIAGADVNEFRGAADPGEVETTIGRAHAIIDRLEALKIPTVAVIHGFCLGGGLEVALACRTRIAIDNARFGFPEVMLGLHPGLGGTVRFTRLVNPMQAMTLMLTGKTIDARKAKSLGLADAVTQERHVRNAVKDAVAGRLKRGRPSVLNGLLNLGPVRGFLGTRMRREAAKAAPQEHYPAPYALIDLWEKHGGSKAAMLAAEKRSFAELMVTSTAQNLIRVFFLREQMKKLAGSGNTVKHLHVIGAGAMGGDIAAWCAHQDIRVTLADMKPEPIAAAIKRAAGLYGKIVRKRTGIRDRLDRLVPDLSGDGVRQADLIIEAVSEKLELKQKVYAAIEPKMKPGAILATNTSSIPLQELRATLQRPERLVGLHFFNPVSRLQLVEVVSHDAADLQVLKQAMAFVGAIDRLPLPVQSSSGFLVNRALTPYMLEAMVMLDEKIDKTVIDAAAEKFGMPMGPIELADQVGLDICLAVGDELRSKFGDALPQPPDWLREKVAKGELGRKTGKGFYDWREGKADKGEAGDVQVQPEIIDRLMLPISNVCVACLREGIVDDPDLVDGAMIFGTGYAPFRGGPLKYARTRGAENVAANLRGLAEKFGVRFAPDPGWDSFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNF1</accession>
    <name>Q3SNF1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Heat shock protein Hsp20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2940</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316115.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2940"/>
    <dbReference type="EnsemblBacteria" id="ABA06190">
        <property type="protein sequence ID" value="ABA06190"/>
        <property type="gene ID" value="Nwi_2940"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2940"/>
    <dbReference type="eggNOG" id="COG0071">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046737_12_0_5"/>
    <dbReference type="OMA" id="NMRDIIP"/>
    <dbReference type="OrthoDB" id="1523369at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044587">
        <property type="entry name" value="HSP21-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46733">
        <property type="entry name" value="PTHR46733"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="SSF49764"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="57"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA06190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="168" mass="19294" checksum="C7A87E296F44683E" modified="2005-10-11" version="1">MSVRDLIPWGRSNNQVPTAFRDDNRDPFLSLHREMNRLFDDVFRGFDGRLPAFGSLSSFNGAWPNVEISDSDKEIKVTAEIPGLEEKDLEVFLNEGVLTLKGEKRSEVEDKEKQFSERFYGHFERRIPLGIEVEEDKIEARFKNGVLNIVLPKSPKAQSQVKRIAIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNG5</accession>
    <name>Q3SNG5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, XRE family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2926</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316101.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06176">
        <property type="protein sequence ID" value="ABA06176"/>
        <property type="gene ID" value="Nwi_2926"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2926"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066192_28_1_5"/>
    <dbReference type="OMA" id="MISANQL"/>
    <dbReference type="OrthoDB" id="2063007at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="2">
        <location>
            <begin position="7"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="103" mass="11232" checksum="002B17927112F779" modified="2005-10-11" version="1">MISANQLRAARALLDIDQRQMAELADLSVPTIQRMEASDGVIRGNVDSLMKLVSALDNAGIELISPDSQSLAGGRGVRLKEHVTRPGIKQPKPFSSRPLERTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNQ3</accession>
    <name>Q3SNQ3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2836</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316028.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2836"/>
    <dbReference type="EnsemblBacteria" id="ABA06088">
        <property type="protein sequence ID" value="ABA06088"/>
        <property type="gene ID" value="Nwi_2836"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2836"/>
    <dbReference type="HOGENOM" id="CLU_1546028_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="173" mass="18963" checksum="DAE11DAB646A5E8C" modified="2005-10-11" version="1">MKEAGAATHLPDIVIRKPREDRIKMRLILGTHNSHGVSVFRDVQALVEKEAIRTRQNIKITESGQPSLFSEEQIIEFEAQRDGVGCAANMGWATETLLGLVRSQPGIAFESAAPLVMEQVPVRGTHVKDIAVGQHKAGFLRFDLPPGKRKPQADTKLYPATSEAADDRPTSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNT0</accession>
    <name>Q3SNT0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF1232 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_2808</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316006.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2808"/>
    <dbReference type="EnsemblBacteria" id="ABA06061">
        <property type="protein sequence ID" value="ABA06061"/>
        <property type="gene ID" value="Nwi_2808"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2808"/>
    <dbReference type="eggNOG" id="COG3339">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133088_1_0_5"/>
    <dbReference type="OMA" id="PDTPKWA"/>
    <dbReference type="OrthoDB" id="2017850at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010652">
        <property type="entry name" value="DUF1232"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016983">
        <property type="entry name" value="UCP031804"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06803">
        <property type="entry name" value="DUF1232"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031804">
        <property type="entry name" value="UCP031804"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="DUF1232" evidence="5">
        <location>
            <begin position="63"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF06803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA06061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="126" mass="13998" checksum="500FCB96E32EA41F" modified="2005-10-11" version="1">MTASKHSVGFEPADRLAQDRDNVRRRFWSKLKRVVAHVPFAEDLLAAYYCAFDKQTPRHVQASLLGAIAYFVLPLDFVPDMLPVLGFTDDAAVLATAIRIVAAHITPEHREAARTALKRGIENEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNW7</accession>
    <name>Q3SNW7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Response regulator receiver</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2771</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315969.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2771"/>
    <dbReference type="EnsemblBacteria" id="ABA06024">
        <property type="protein sequence ID" value="ABA06024"/>
        <property type="gene ID" value="Nwi_2771"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2771"/>
    <dbReference type="eggNOG" id="COG0784">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089963_0_0_5"/>
    <dbReference type="OMA" id="FFQENAS"/>
    <dbReference type="OrthoDB" id="1133475at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014605">
        <property type="entry name" value="Sig_resp-reg_PhyR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036400">
        <property type="entry name" value="RR_Ctr_UCP036400"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="139"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="268" mass="29172" checksum="ECA5A4DFB1363D2F" modified="2005-10-11" version="1">MFRSQVVAEHLPLLRRYARALTGSQASGDAYVGAMLEALLQDPTLLDEQHGARAGLFRLFTRIWNSVSVNGGADEVPTLAPTERRLTNITPLPRQAFLLLSLEGFSEDEAAFVLEVDIAEVRRLSGIAGREMAAEIATDVLIIEDETFIAMDLESLVKNLGHRVIGVARTHADAIALAKTKEPGLILADIQLADGSSGLDAVNELLRVVEVPVVFITAYPERFLTGERPEPAFLISKPFQPAMVSAVASQALFFQRNSRNREPRAAAS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNX3</accession>
    <name>MASZ_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Malate synthase G</fullName>
            <ecNumber evidence="1">2.3.3.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">glcB</name>
        <name type="ordered locus">Nwi_2765</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18181"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the malate synthase family. GlcB subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.9" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315963.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SNX3"/>
    <dbReference type="SMR" id="Q3SNX3"/>
    <dbReference type="STRING" id="323098.Nwi_2765"/>
    <dbReference type="PRIDE" id="Q3SNX3"/>
    <dbReference type="EnsemblBacteria" id="ABA06018">
        <property type="protein sequence ID" value="ABA06018"/>
        <property type="gene ID" value="Nwi_2765"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2765"/>
    <dbReference type="eggNOG" id="COG2225">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028446_1_0_5"/>
    <dbReference type="OMA" id="MAPGFDG"/>
    <dbReference type="OrthoDB" id="322024at2"/>
    <dbReference type="UniPathway" id="UPA00703">
        <property type="reaction ID" value="UER00720"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004474">
        <property type="term" value="F:malate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1220.12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.360">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00641">
        <property type="entry name" value="Malate_synth_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044856">
        <property type="entry name" value="Malate_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011076">
        <property type="entry name" value="Malate_synth_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001465">
        <property type="entry name" value="Malate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006253">
        <property type="entry name" value="Malate_synthG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046363">
        <property type="entry name" value="MS_N_TIM-barrel_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42739">
        <property type="entry name" value="PTHR42739"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01274">
        <property type="entry name" value="Malate_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51645">
        <property type="entry name" value="SSF51645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01345">
        <property type="entry name" value="malate_syn_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0329">Glyoxylate bypass</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0558">Oxidation</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="chain" id="PRO_1000056914" description="Malate synthase G">
        <location>
            <begin position="1"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="337"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="628"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="124"/>
            <end position="125"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="429"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="429"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="454"/>
            <end position="457"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="457"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="538"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Cysteine sulfenic acid (-SOH)" evidence="1">
        <location>
            <position position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00641"/>
        </source>
    </evidence>
    <sequence length="741" mass="81247" checksum="304959E432A852B2" modified="2005-10-11" version="1">MNRIDAHGLKIAPVLFDFIAGEATLRTGIKPDAFWAGLAAIVRDLGPRNRELLAVRDRLQARIDDWHRIHKDTPFDISAYTAFLTEIGYLEPEPETRQIETANVDDEIGKICGPQLVVPLTNARYALNAANARWGSLYDALYGTDAIAREAGEAKGYDKARGDKVIARAKAFLDMATPLANGAHADVAGYSVLNGRLSVTLRNGDTTGLKAETQFAGYQGDANAPSAILLVNHGLHIDIQIDRAHPIGRHYSAGVADVIIEAAISTILDMEDSVAAVDADDKVLVYRNALGLMNGTLTDTFEKSGKTLTRALNPDRVYTAPDGGQLTLHGRSLLLIRNVGHHMFTDAVLDQRGAEIPEGLLDAAISGLLAIHDINGVSKRRNSRSGSLYLVKPKMHGPDEVALTCELFARVEAMLGLRENTLKIGIMDEERRTTVNLNACIQTASKRVCFINTGFLDRTGDEIHTSMEAGPMIRKGEMKTQPWIKAYEDWNVDIGLIEGLPGRAQIGKGMWAAPDRMADMLAQKINHPEAGATTAWVPSPTAATLHALHYHQIDVRKRQQELKAGGRRARLSDLLTIPVSHSNWPPEDVKQEIDNNCQGILGYVVRWIDQGVGCSKVPDIHDVGLMEDRATLRISSQHLANWLHHGVVTHDQVMDSFKRMAVIVDEQNAGDPLYKPMAPGFDSVAFKAACDLVFRGREQPNGYTEHILTERRREAKLMAQAARAFSGEADTGSPRENATRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP29</accession>
    <name>Q3SP29_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pyruvate, phosphate dikinase</fullName>
            <ecNumber evidence="5">2.7.9.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Pyruvate, orthophosphate dikinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">Nwi_2709</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the reversible phosphorylation of pyruvate and phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + phosphate + pyruvate = AMP + diphosphate + H(+) + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:10756"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.9.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PEP-utilizing enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.7.9.1" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2709"/>
    <dbReference type="EnsemblBacteria" id="ABA05962">
        <property type="protein sequence ID" value="ABA05962"/>
        <property type="gene ID" value="Nwi_2709"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2709"/>
    <dbReference type="eggNOG" id="COG0574">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1080">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015345_0_2_5"/>
    <dbReference type="OMA" id="RRFVQMY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050242">
        <property type="term" value="F:pyruvate, phosphate dikinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:pyruvate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008279">
        <property type="entry name" value="PEP-util_enz_mobile_dom"/>
    </dbReference>
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        <property type="entry name" value="PEP_util_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000121">
        <property type="entry name" value="PEP_util_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023151">
        <property type="entry name" value="PEP_util_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036637">
        <property type="entry name" value="Phosphohistidine_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002192">
        <property type="entry name" value="PPDK_AMP/ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010121">
        <property type="entry name" value="Pyruvate_phosphate_dikinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22931">
        <property type="entry name" value="PTHR22931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00391">
        <property type="entry name" value="PEP-utilizers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02896">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="pyru_phos_dikin"/>
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        <property type="entry name" value="PEP_ENZYMES_2"/>
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        <property type="entry name" value="PEP_ENZYMES_PHOS_SITE"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PPDK_N" evidence="14">
        <location>
            <begin position="157"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilizers" evidence="13">
        <location>
            <begin position="572"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilizers_C" evidence="15">
        <location>
            <begin position="666"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="98"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="9">
        <location>
            <position position="604"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9">
        <location>
            <position position="980"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="710"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="766"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 11">
        <location>
            <position position="894"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="915"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
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    <feature type="binding site" evidence="10">
        <location>
            <position position="916"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
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    <feature type="binding site" evidence="10">
        <location>
            <position position="917"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 11">
        <location>
            <position position="918"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000853-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000853-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000853-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ABA05962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1028" mass="111744" checksum="97F3298D3E54292F" modified="2005-10-11" version="1">MSPELESSVDLLRNKASLRSLRGVAEIWCRAIALSPDLLFLDPDSFIASRKGERLLVLCFDAFSSREPEFPSSGQARRHASPENAMERFQTMAKAASKSKKLATKSGSSAKFRAKARKSVGTGKPTRNAQGALPRSKSKWVYIFGGGKAEGKAALKELLGGKGANLAEMANLGLPVPPGFTITTAVCAFFYAHDKSYPKELKPQVEKALEQVARITGRAFGDATNPLLVSVRSGARASMPGMMDTVLNLGLNDRTVEAIAARSDDRRFAYDSYRRFITMYSDVVLGFAHHHFEEILDTFKDGRGYSLDTDLTGDDWIEVVGRYKTAVARETGKDFPQDPHQQLWGAIGAVFSSWMSARAVTYRRLHNIPESWGTAVNVQAMVFGNLGETSATGVAFTRNPSTGESRLYGEFLINAQGEDVVAGIRTPQDLTEVARKESGSNKPSMETAMPESFKELTRISRLLEEHYRDMQDMEFTVEDGKLWMLQTRSGKRTTRAALRIAVELARGGLISKRDAVARIDPASLDQLLHPTIDPNATRDVIAAGLPASPGAAAGEIVFSSEEATRLQADGRKVILVRIETSPEDIHGMHAAEGILTTRGGMTSHAAVVARGMGKPCVCGCGSIRVDYGGGVMTIGDRTFKVGDVITIDGSLGEVLAGQMPMIEPELSGEFGTLMGWADEIRKLGIRVNGDTSADARTALRFGAEGIGLCRTEHMFFEETRIRTVREMILAEDEQARRAALSKLLPMQRADFVDLFEIMKGLPVTIRLLDPPLHEFLPHTQAEIEEVARSMKADPRKLADRARNLAEFNPMLGFRGCRLAIVYPEIAEMQARAIFEAAIEAEKRTGKAVAVEVMVPLIATKAEFDLIKARIDMTAQSVMRETGGKPTYQVGTMIELPRACLVAGEIAETAEFFSFGTNDLTQTTFGISRDDASSFLGAYIAKGVVAIDPFISLDRGGVGELVKIGVARGRKTRPNLKVGICGEHGGDPVSVAFCHEIGLNYVSCSPYRVPIARLAAAHAALGKIVAGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP82</accession>
    <name>Q3SP82_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Multisubunit potassium/proton antiporter, PhaD subunit</fullName>
            <ecNumber evidence="4">1.6.99.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2656</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.6.99.5" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315859.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2656"/>
    <dbReference type="EnsemblBacteria" id="ABA05909">
        <property type="protein sequence ID" value="ABA05909"/>
        <property type="gene ID" value="Nwi_2656"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2656"/>
    <dbReference type="eggNOG" id="COG0651">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007100_9_2_5"/>
    <dbReference type="OMA" id="YVAHHIT"/>
    <dbReference type="OrthoDB" id="1887859at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_mem"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="73"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="110"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="163"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="246"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="281"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="307"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="334"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="387"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="436"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="477"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="Proton_antipo_M" evidence="3">
        <location>
            <begin position="130"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="535" mass="55818" checksum="4B50724028308D87" modified="2005-10-11" version="1">MNNLMIAPVVLPLFAGAIMLALGGEQRRNISAALNIITSFALVAISIILLRAADVAPTGVAGVYRLGDWPAPFAIVLVVDRLATLMLLLTSLLAAAAAVFSLARWHRAGVLFHPLFQFMLMGINGAFLTGDLFNLFVFIEVLLAASYGLALHGSGSARVKAGLHYIAVNLVASLLFLIGVSLIYGVTGTLNMADLATRIPAIGAQDRALLEAGAGILGIAFLVKAGMWPLCFWLPGTYAAAPPPVAAIFAVLTKVGAYIIIRLSLLLFGDGTGASAQFGDQLLLMGGMATVAFGAIGSLASQDMARLASFSVLVSSGTILATIGLGQVGVTGAALFYLISSTLGLGAFFLLIELVERDREPGADVLAVTRELYGDEDDSENSQETGIAIPATIGILGVAFISCALVISGMPPLSGFVAKFAILTTALGSPANELPLAIWIFLFLLIVSGLAALIAMTRAGIRSFWASPDRTIPRVRLIEMAPVAVLLLLCAIQTVEAGPIMRFMQATAQSLHAPHHYVRGVLDSPAKPSDSRGPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPB1</accession>
    <name>Q3SPB1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF58 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2627</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2627"/>
    <dbReference type="EnsemblBacteria" id="ABA05880">
        <property type="protein sequence ID" value="ABA05880"/>
        <property type="gene ID" value="Nwi_2627"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2627"/>
    <dbReference type="eggNOG" id="COG1721">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054927_3_0_5"/>
    <dbReference type="OMA" id="ARDDHLY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002881">
        <property type="entry name" value="DUF58"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01882">
        <property type="entry name" value="DUF58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF58" evidence="1">
        <location>
            <begin position="56"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="313" mass="34581" checksum="348FA9F753A7A6FF" modified="2005-10-11" version="1">MVAAAGHVRQEIEAVRRADGESRTLAASLPRLVLEARRIAANVIHGLHGRRRPGPGESFWQYRRFVSGEPSQNVDWRRSARDDHLYVREHEWEAAHTVWIWPDRSASMAFASRHARDSKLERALIVTFALAELLVAGGERVGVPGLVNPTASRDVIDRIAQAMLHDDRARASLPPSFVPSARSEIVLLSDFWSPVADIRTMLAGLSASGAHGTLLQVVDPAEETFPYSGRVEFVDPEDGRVITAGRAERWASDYVRRVALHRDQIRTETKALDWLFSTHTTDRSAAELLLFLHSGMMASRGSGGVSAGAERTA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPC5</accession>
    <name>APT_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Adenine phosphoribosyltransferase</fullName>
            <shortName evidence="1">APRT</shortName>
            <ecNumber evidence="1">2.4.2.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">apt</name>
        <name type="ordered locus">Nwi_2613</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + adenine</text>
            <dbReference type="Rhea" id="RHEA:16609"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.4.2.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.7" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315816.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SPC5"/>
    <dbReference type="SMR" id="Q3SPC5"/>
    <dbReference type="STRING" id="323098.Nwi_2613"/>
    <dbReference type="EnsemblBacteria" id="ABA05866">
        <property type="protein sequence ID" value="ABA05866"/>
        <property type="gene ID" value="Nwi_2613"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2613"/>
    <dbReference type="eggNOG" id="COG0503">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063339_3_0_5"/>
    <dbReference type="OMA" id="KPGIVFR"/>
    <dbReference type="OrthoDB" id="1532478at2"/>
    <dbReference type="UniPathway" id="UPA00588">
        <property type="reaction ID" value="UER00646"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003999">
        <property type="term" value="F:adenine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006168">
        <property type="term" value="P:adenine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044209">
        <property type="term" value="P:AMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00004">
        <property type="entry name" value="Aden_phosphoribosyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005764">
        <property type="entry name" value="Ade_phspho_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01090">
        <property type="entry name" value="apt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00103">
        <property type="entry name" value="PUR_PYR_PR_TRANSFER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0660">Purine salvage</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000000316" description="Adenine phosphoribosyltransferase">
        <location>
            <begin position="1"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00004"/>
        </source>
    </evidence>
    <sequence length="179" mass="19308" checksum="E2B79434070DB645" modified="2005-10-11" version="1">MTFEDDLQASVRTIPDYPKPGVMFRDITTLLGDARAFRRAVDQLVHPWAGSKIDKVAGIEARGFILGGAVAHQVSAGFVPIRKKGKLPHQTVRMAYALEYGTDEMEMHVDAIARGERVILVDDLIATGGTAEGAVKLLRQIGADVVAACFIIDLPDLGGAAKLRAMDVPVRTLMAFEGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPH6</accession>
    <name>Q3SPH6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">4-hydroxybenzoate octaprenyltransferase</fullName>
            <ecNumber evidence="8">2.5.1.39</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">4-HB polyprenyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ubiA</name>
        <name evidence="9" type="ordered locus">Nwi_2562</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the prenylation of para-hydroxybenzoate (PHB) with an all-trans polyprenyl group. Mediates the second step in the final reaction sequence of ubiquinone-8 (UQ-8) biosynthesis, which is the condensation of the polyisoprenoid side chain with PHB, generating the first membrane-bound Q intermediate 3-octaprenyl-4-hydroxybenzoate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>4-hydroxybenzoate + all-trans-octaprenyl diphosphate = 4-hydroxy-3-all-trans-octaprenylbenzoate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:27782"/>
            <dbReference type="ChEBI" id="CHEBI:1617"/>
            <dbReference type="ChEBI" id="CHEBI:17879"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57711"/>
            <dbReference type="EC" id="2.5.1.39"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell inner membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the UbiA prenyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.39" evidence="8"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315766.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2562"/>
    <dbReference type="EnsemblBacteria" id="ABA05815">
        <property type="protein sequence ID" value="ABA05815"/>
        <property type="gene ID" value="Nwi_2562"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2562"/>
    <dbReference type="eggNOG" id="COG0382">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034879_0_2_5"/>
    <dbReference type="OMA" id="MVVYPYG"/>
    <dbReference type="OrthoDB" id="1472516at2"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002083">
        <property type="term" value="F:4-hydroxybenzoate decaprenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047293">
        <property type="term" value="F:4-hydroxybenzoate nonaprenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008412">
        <property type="term" value="F:4-hydroxybenzoate octaprenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13959">
        <property type="entry name" value="PT_UbiA_COQ2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01635">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006370">
        <property type="entry name" value="HB_polyprenyltransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039653">
        <property type="entry name" value="Prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030470">
        <property type="entry name" value="UbiA_prenylTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11048">
        <property type="entry name" value="PTHR11048"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01474">
        <property type="entry name" value="ubiA_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00943">
        <property type="entry name" value="UBIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="106"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="162"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="231"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="307" mass="33289" checksum="77C1A439432744C2" modified="2005-10-11" version="1">MSDAAARVADSVGNWVDSRAPLWSRPYLRLSRLDRPIGSWLLLMPCLWSTALASHLAGDVSQLPFTIVLFLIGAFAMRGAGCTWNDITDRDLDARVERTRSRPIPAGQVTVTQALVFLVAQALAGLIVLLQFNRFAIATGIASLLIVAAYPFMKRITYWPQVVLGLAFSWGALMGFAVVLGRIDAAALALYAGTVAWVIGYDTIYAHQDADDDALIGIKSTALLFGERTRLALAIFYGLAVVLISVALHLAEALWPAWIGLAVFAAHLVWQIARIEISDARLCLRLFKSNRDAGVLLFAGLLIGALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPH7</accession>
    <name>Q3SPH7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2561</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315765.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2561"/>
    <dbReference type="EnsemblBacteria" id="ABA05814">
        <property type="protein sequence ID" value="ABA05814"/>
        <property type="gene ID" value="Nwi_2561"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2561"/>
    <dbReference type="eggNOG" id="ENOG5032YZE">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096801_0_0_5"/>
    <dbReference type="OMA" id="RRPRFLM"/>
    <dbReference type="OrthoDB" id="1874148at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046083">
        <property type="entry name" value="DUF6101"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19596">
        <property type="entry name" value="DUF6101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="173" mass="19288" checksum="615AAAC306BC130D" modified="2005-10-11" version="1">MRRQTAISGINPAGSSRSLRLDPLSLPVSFAANDSRADGGIRQIELHRERVTLHRAVRGMKMTISVRVSEFIGVTVRDTSHGRALVLLHRDPSLAIPLLVTEDAGECAAAWQTWSEVLALPQLADDTREPAPRRRRRNAISDRRPAFLVRRRCGHSLNPAAIHRGEREIIARD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPN1</accession>
    <name>Q3SPN1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MaoC-like dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2507</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315711.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2507"/>
    <dbReference type="EnsemblBacteria" id="ABA05760">
        <property type="protein sequence ID" value="ABA05760"/>
        <property type="gene ID" value="Nwi_2507"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2507"/>
    <dbReference type="eggNOG" id="COG2030">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094876_1_0_5"/>
    <dbReference type="OMA" id="ISSGWMT"/>
    <dbReference type="OrthoDB" id="1563802at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002539">
        <property type="entry name" value="MaoC-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01575">
        <property type="entry name" value="MaoC_dehydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MaoC-like" evidence="1">
        <location>
            <begin position="19"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="161" mass="17973" checksum="FE6D4B77583DBC9D" modified="2005-10-11" version="1">MAYFEDMEVGLRRDLGSFTFTGERIKAFASQFDPQPFHLDEEEGRRSLFGGLAASGWHVASVWMKLMVADSRREAAEAAARGEKPGMWGPSPGFRDMRWLKPVLAGDTVSYASEVESLRASGSRPEWGVMQVRNTGTNQRGELVFSFLGSVFVPRRHPSQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPS6</accession>
    <name>Q3SPS6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2462</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2462"/>
    <dbReference type="EnsemblBacteria" id="ABA05715">
        <property type="protein sequence ID" value="ABA05715"/>
        <property type="gene ID" value="Nwi_2462"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2462"/>
    <dbReference type="eggNOG" id="ENOG50313J9">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2058908_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="119" mass="13373" checksum="44D32198C219E069" modified="2005-10-11" version="1">MRMELIQRAAPCCRARALRRQLVVRGNPAKEQRGFSASCALQLQTSKTDGRRAMLAAGSDDLPHQNPCAQCGRLIAAPVWFEKEPCRTSYLWRCVRCGYKFEAIAFFKQANHESRATAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPV9</accession>
    <name>Q3SPV9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PRC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2428</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2428"/>
    <dbReference type="EnsemblBacteria" id="ABA05682">
        <property type="protein sequence ID" value="ABA05682"/>
        <property type="gene ID" value="Nwi_2428"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2428"/>
    <dbReference type="eggNOG" id="COG1873">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108884_2_0_5"/>
    <dbReference type="OMA" id="MGSINPQ"/>
    <dbReference type="OrthoDB" id="1849143at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="SSF50346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRC" evidence="2">
        <location>
            <begin position="18"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="137" mass="14915" checksum="35F631AE353E3C47" modified="2005-10-11" version="1">MAMEGNSESLIGSDKESGKAVYGAEDKKIGSIERVMIDKSSGEPAYAVLSVGGFLGVGDEHYPVPWRLLRYDSELDGYRSDISGNRLKGYPKSVFGSSGSELFDWTARRAELDSYYDNIDTVTTKEGAESPLTRNSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPY1</accession>
    <name>Q3SPY1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NAD-dependent epimerase/dehydratase</fullName>
            <ecNumber evidence="2">4.2.1.46</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2406</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.2.1.46" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315621.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2406"/>
    <dbReference type="EnsemblBacteria" id="ABA05660">
        <property type="protein sequence ID" value="ABA05660"/>
        <property type="gene ID" value="Nwi_2406"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2406"/>
    <dbReference type="eggNOG" id="COG0451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_7_5"/>
    <dbReference type="OMA" id="KLAMEHM"/>
    <dbReference type="OrthoDB" id="1180629at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008460">
        <property type="term" value="F:dTDP-glucose 4,6-dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd05257">
        <property type="entry name" value="Arna_like_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045869">
        <property type="entry name" value="Arna-like_SDR_e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026390">
        <property type="entry name" value="EDH_00030"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04180">
        <property type="entry name" value="EDH_00030"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="1">
        <location>
            <begin position="7"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="334" mass="36449" checksum="A480B22DA350E45B" modified="2005-10-11" version="1">MTSRKVLVTGAAGFIGSHLVEELVRRNLTVRAFVHYNSMGSRGWLDMSPRYIRDALDVFAGDIRDPNGVRDAMKGCDAVFHLAALIAIPYSYHSPDTYVDTNIRGTLNVVQAARDLGVAHVVHTSTSEVYGTAQFVPITEGHPLQGQSPYSASKIGADQIALSFERSFGTPVTVVRPFNTYGPRQSARAVIPAIITQIAGGARVIRLGSTEPTRDFSFVSDTVAGFIAADEQRAAIGEVVNLGSGFEVSIGNTAELIAEIMGVEIQIELDQSRQRPARSEVDRLFASNTKAQKLLSWTPQFAGLDGFRRGIEKTVPWFRDPVNLSRYRTSAYTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ15</accession>
    <name>Q3SQ15_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2372</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2372"/>
    <dbReference type="TCDB" id="1.B.57.3.2">
        <property type="family name" value="the legionella major-outer membrane protein (lm-omp) family"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05626">
        <property type="protein sequence ID" value="ABA05626"/>
        <property type="gene ID" value="Nwi_2372"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2372"/>
    <dbReference type="eggNOG" id="ENOG5032M60">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_920798_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229046" evidence="3">
        <location>
            <begin position="28"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="30"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="302" mass="31897" checksum="25CB66BE73751AC8" modified="2005-10-11" version="1">MNTTTRLSTLLLSTAAFVAVACGASLAADENSLQHRLAQLERENATLTRENAALRLNRKLARENVALRKSSPERAAPAAAPSPGTSVRDAYAADFPVAYKALPVETQGQFRFWGEGGAIWSGGDPVTTPYSLRGFSSDLNFGFGAAGGPPNRLFNLTPKVGWEAAAGFDYRFAGSPWHVSGEFRYGQARGSGADASAGTIDPAVLALLAAANPVFAGVAIGGSQTIAASHREDRWQADIALGYDALGGPPMPCRSRAACVSPNWSGARPSSIEAKPTCGFQRRWISASAYRSRALITARRRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ53</accession>
    <name>Q3SQ53_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2334</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2334"/>
    <dbReference type="EnsemblBacteria" id="ABA05588">
        <property type="protein sequence ID" value="ABA05588"/>
        <property type="gene ID" value="Nwi_2334"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2334"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155201_0_0_5"/>
    <dbReference type="OMA" id="GERANKQ"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021880">
        <property type="entry name" value="DUF3489"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11994">
        <property type="entry name" value="DUF3489"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="108" mass="11803" checksum="558D8D3D44B2BFE5" modified="2005-10-11" version="1">MQKSSRNRKSWKRASRAKAGRGAKKAKAARQSARHAAGERANKQTKLLDLLRRPAGASIAALSEASGWQQHSVRGFLAGVVRKKLGLELVSSIEADQRIYRIVAAKQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQC6</accession>
    <name>Q3SQC6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Type-4 uracil-DNA glycosylase</fullName>
            <ecNumber evidence="3">3.2.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_2261</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil.</text>
            <dbReference type="EC" id="3.2.2.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the uracil-DNA glycosylase (UDG) superfamily. Type 4 (UDGa) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.27" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2261"/>
    <dbReference type="EnsemblBacteria" id="ABA05515">
        <property type="protein sequence ID" value="ABA05515"/>
        <property type="gene ID" value="Nwi_2261"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2261"/>
    <dbReference type="eggNOG" id="COG1573">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044815_1_3_5"/>
    <dbReference type="OMA" id="NCRGYLE"/>
    <dbReference type="OrthoDB" id="1529383at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005273">
        <property type="entry name" value="Ura-DNA_glyco_family4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00986">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="SSF52141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00758">
        <property type="entry name" value="UDG_fam4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03914">
        <property type="entry name" value="UDG_fam_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDG" evidence="10">
        <location>
            <begin position="53"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA05515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="225" mass="25057" checksum="8753262DF3516E70" modified="2005-10-11" version="1">MIATEATMPRKPQKREQPMARQSSAHDSLPSLREEAAGCRACPLWKDATQTVFGEGLAHAQVMLVGEQPGDKEDLAGKPFIGPAGQMLDRALKEAGIDRTKVYVTNAVKHFKFVPRGKIRLHQKPNTPEIRACRPWYERELAAVNPALVVAMGSTAAQSVFGKITPINKSRGRLIDMKDGRKALVTVHPSYLLRLPDQDDRAREYKRFVDDLKIAATHLQRDRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQC8</accession>
    <name>Q3SQC8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phage SPO1 DNA polymerase-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2259</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315481.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2259"/>
    <dbReference type="EnsemblBacteria" id="ABA05513">
        <property type="protein sequence ID" value="ABA05513"/>
        <property type="gene ID" value="Nwi_2259"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2259"/>
    <dbReference type="eggNOG" id="COG1573">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046101_0_0_5"/>
    <dbReference type="OMA" id="HEPAHDI"/>
    <dbReference type="OrthoDB" id="1529383at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023875">
        <property type="entry name" value="DNA_metab_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025404">
        <property type="entry name" value="DUF4130"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13566">
        <property type="entry name" value="DUF4130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03915">
        <property type="entry name" value="SAM_7_link_chp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4130" evidence="1">
        <location>
            <begin position="80"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="247" mass="28891" checksum="B4C08217F3BCEEDA" modified="2005-10-11" version="1">MTRHIILDGETDFDGWRRAARALALNNAAPHDVVWRAPGNEPDLFTPAAAALPDGPFVSTFNIPARFIELAQAAILHRDPERFALLYRLLWRLRMNHDLLDIATDQDVVRIRDMVRAVRRDEHKMHAFVRFREIGRERDTHFIAWFEPEHHIVELAAPFFMRRFADMNWSILTPDTCAHWNDNRLSFTPGVCRDEAPAGDRLEEVWRSYYASIFNTARLKVKAMQTEMPKKYWRNLPEASLIRVFLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQD8</accession>
    <name>Q3SQD8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2249</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2249"/>
    <dbReference type="EnsemblBacteria" id="ABA05503">
        <property type="protein sequence ID" value="ABA05503"/>
        <property type="gene ID" value="Nwi_2249"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2249"/>
    <dbReference type="HOGENOM" id="CLU_2570362_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="81" mass="9003" checksum="6AA1ACD9292D3C21" modified="2005-10-11" version="1">MADQAVENVIEERDLLVGIIDRAVQEQVGDPPQRFDPPRDRPWTRTVWSSSSRSADPADLEAMALSWSGSVPVSAAMFRSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQH6</accession>
    <name>Q3SQH6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Amino acid/amide ABC transporter ATP-binding protein 1, HAAT family</fullName>
            <ecNumber evidence="6">3.6.3.29</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_2211</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in beta-(1--&gt;2)glucan export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.29" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315434.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2211"/>
    <dbReference type="EnsemblBacteria" id="ABA05465">
        <property type="protein sequence ID" value="ABA05465"/>
        <property type="gene ID" value="Nwi_2211"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2211"/>
    <dbReference type="eggNOG" id="COG0411">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_5"/>
    <dbReference type="OMA" id="EHDMRFI"/>
    <dbReference type="OrthoDB" id="1179151at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032823">
        <property type="entry name" value="BCA_ABC_TP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12399">
        <property type="entry name" value="BCA_ABC_TP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="8"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="40"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="278" mass="30079" checksum="67808CC63AC14603" modified="2005-10-11" version="1">MTTPAPILDVDGLTMRFGGILAINELSFTAQRRHITALIGPNGAGKTTVFNCVTGFYRPSRGAMRLVHDDGATFRLDRLRDFRICKYAKVARTFQNIRLFPGMTALENLMVAQHNALMRASGFSLLGLIGAPSFRAAEKQAIDSARDWLDRIGLLQRADDAAGNLPYGDQRRLEIARAMCAQPALLCLDEPAAGLNANESAGLSELLLSIRSDFGTSILLIEHDMSVVMEISDHIIVLDHGVKIADGTPRAVRDDPKVVAAYLGADEDTAAAVMEGDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQU5</accession>
    <name>Q3SQU5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">AAA ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2089</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315324.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2089"/>
    <dbReference type="EnsemblBacteria" id="ABA05346">
        <property type="protein sequence ID" value="ABA05346"/>
        <property type="gene ID" value="Nwi_2089"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2089"/>
    <dbReference type="eggNOG" id="COG0464">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_25_0_5"/>
    <dbReference type="OMA" id="AYHCRES"/>
    <dbReference type="OrthoDB" id="1115436at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="4">
        <location>
            <begin position="122"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="336" mass="36914" checksum="E8CC5633800E2E52" modified="2005-10-11" version="1">MSTKHLLALLNSHIEGDEDQFLSIALQLAAQEARQGRGEEAEKLKRLVQKARDQQRAGRPAGGQTPIPLARPRGELQGLVESAYPKVTLASMVLADDVRARLNRVVRQQQERATLREHGQAPSTHMLLVGPPGTGKTMTASALAGELRLPLFTVRLESLFSRFFGETAGKLRLLFDQIAQTRGVYLLDEFDAIGARRGEPNDVGEIRRVLNSVLAFMEEPNSTDSLVLAATNHVEILDQALARRFDEVIEYTLPDVAAARAIMEGRLGKFKIALKSWATLEPALEGLSQGELVRAAHAVVKDAILEGATKVSAEALREALHNRQTLKGKFRRQSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQX9</accession>
    <name>Q3SQX9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2054</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315293.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05312">
        <property type="protein sequence ID" value="ABA05312"/>
        <property type="gene ID" value="Nwi_2054"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2054"/>
    <dbReference type="eggNOG" id="ENOG5032K12">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1914925_0_0_5"/>
    <dbReference type="OMA" id="ACHKRFL"/>
    <dbReference type="OrthoDB" id="1928905at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021791">
        <property type="entry name" value="Phage_TAC_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11836">
        <property type="entry name" value="Phage_TAC_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="137" mass="14430" checksum="C53E36B167236B7D" modified="2005-10-11" version="1">MTKQSTNPCARQISFAGKVRTFNLNDPKVSAMIAGGAKLKQVARMITISRLGIGRAPLAGPYGDTAAACHKRFLEKIYSATDVENVITLGLIGGGMPEDEAFALVDEHVVGKPLEANALIASETIAALFADKEDAQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQZ3</accession>
    <name>Q3SQZ3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Biopolymer transport protein ExbD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_2039</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the TonB-dependent energy-dependent transport of various receptor-bound substrates.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">The accessory proteins ExbB and ExbD seem to form a complex with TonB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the ExbD/TolR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315281.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2039"/>
    <dbReference type="EnsemblBacteria" id="ABA05298">
        <property type="protein sequence ID" value="ABA05298"/>
        <property type="gene ID" value="Nwi_2039"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2039"/>
    <dbReference type="eggNOG" id="COG0848">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085305_1_3_5"/>
    <dbReference type="OMA" id="GRFAINQ"/>
    <dbReference type="OrthoDB" id="1940365at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003400">
        <property type="entry name" value="ExbD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014170">
        <property type="entry name" value="TonB_ExbD_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30558">
        <property type="entry name" value="PTHR30558"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02472">
        <property type="entry name" value="ExbD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02803">
        <property type="entry name" value="ExbD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA05298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="156" mass="16550" checksum="F10C29D37F72FFC8" modified="2005-10-11" version="1">MGGSLKNPQDGGIDELSEINVTPFIDVMLVLLIIFMVAAPLSTVDVAVDLPVSNAQPQPRPDKPVFLTVKQDLTLALGNDDVPRNTLQATLDQQTENDREQRVFLRADGTVAYTELMKVMNLLREAGYLKIALVGLEDTGQAVAAGGAAPAAAPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQZ6</accession>
    <name>Q3SQZ6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative NAD(P)H nitroreductase</fullName>
            <ecNumber evidence="2">1.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_2036</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="3">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the nitroreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.-.-.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315279.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2036"/>
    <dbReference type="EnsemblBacteria" id="ABA05295">
        <property type="protein sequence ID" value="ABA05295"/>
        <property type="gene ID" value="Nwi_2036"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2036"/>
    <dbReference type="eggNOG" id="COG0778">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070764_5_0_5"/>
    <dbReference type="OMA" id="IPEWEQV"/>
    <dbReference type="OrthoDB" id="1847197at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02135">
        <property type="entry name" value="YdjA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026021">
        <property type="entry name" value="YdjA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000232">
        <property type="entry name" value="YdjA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="SSF55469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2 3" id="KW-0288">FMN</keyword>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="5">
        <location>
            <begin position="8"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="11"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="136"/>
            <end position="138"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR000232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000232-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="187" mass="20701" checksum="FDDED1DB351211D5" modified="2005-10-11" version="1">MSEIIDFLRTRRSVKPREMSQPAPSPEEIETILTIGARVPDHGKIAPWRFIVFEGDGRVRAGDVIASVYARKNPSAPQEEIEIEKRQLMEAPLVIGVVSCIKPHPKVPSWEQVLSAGASAMNIVTAVTALGYGANWLTGWFAYDRDILDGLGLKPDERFAGFIHIGTPTKPSKDRPRPNLSDIVSRF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR12</accession>
    <name>DER_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GTPase Der</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">GTP-binding protein EngA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">der</name>
        <name type="synonym">engA</name>
        <name type="ordered locus">Nwi_2020</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">GTPase that plays an essential role in the late steps of ribosome biogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Associates with the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. EngA (Der) GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315265.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SR12"/>
    <dbReference type="SMR" id="Q3SR12"/>
    <dbReference type="STRING" id="323098.Nwi_2020"/>
    <dbReference type="PRIDE" id="Q3SR12"/>
    <dbReference type="EnsemblBacteria" id="ABA05279">
        <property type="protein sequence ID" value="ABA05279"/>
        <property type="gene ID" value="Nwi_2020"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2020"/>
    <dbReference type="eggNOG" id="COG1160">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016077_5_0_5"/>
    <dbReference type="OMA" id="KFRFLEY"/>
    <dbReference type="OrthoDB" id="263682at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00195">
        <property type="entry name" value="GTPase_Der"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031166">
        <property type="entry name" value="G_ENGA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016484">
        <property type="entry name" value="GTP-bd_EngA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032859">
        <property type="entry name" value="KH_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14714">
        <property type="entry name" value="KH_dom-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006485">
        <property type="entry name" value="GTP-binding_EngA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03594">
        <property type="entry name" value="GTPase_EngA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51712">
        <property type="entry name" value="G_ENGA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="chain" id="PRO_1000011681" description="GTPase Der">
        <location>
            <begin position="1"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="EngA-type G 1">
        <location>
            <begin position="3"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="EngA-type G 2">
        <location>
            <begin position="184"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="KH-like" evidence="1">
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        <location>
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        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>1</label>
        </ligand>
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        <location>
            <begin position="56"/>
            <end position="60"/>
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        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>1</label>
        </ligand>
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    <feature type="binding site" evidence="1">
        <location>
            <begin position="119"/>
            <end position="122"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>1</label>
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        <location>
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        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
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            <begin position="237"/>
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        <ligand>
            <name>GTP</name>
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        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
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        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00195"/>
        </source>
    </evidence>
    <sequence length="455" mass="50150" checksum="001BF228B06328F9" modified="2005-10-11" version="1">MSFTIAIIGRPNVGKSTLFNRLVGQKLALVDDEPGVTRDRREGQARLGDLDFTVIDTAGLDEGPRGSLTARMQEQTEAAIAAADALMFVFDARAGLTPTDRSFADFARRADKPVVLVANKSEGRHGDAGALESYALGLGDPVGVSAEHDEGMSDLYDALRSVMPEPAEEVDEEEIVEPDMSRPIRVAIVGRPNAGKSTVINYLLSEERLLTSPEAGTTRDSISVELNWKGRDFRIFDTAGLRRRSRIEAKLEKLSVADTLRAVRFAEAVVLMMDAQNRFEEQDLRIADLIEREGRALVIAVNKWDLMKGGSARIASLRNDVDHWLPQIRGAPVVAISGLTGEGIDRLMIAIQTAYAVWNRRVATALLNRWFQQAVAASPPPAVSGRRLKLNYATQTKARPPSFVVFCSRADAVPESYLRYLVNSLRETFDLAGTPIRITLREKANPFAHKRKRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRD5</accession>
    <name>Q3SRD5_NITWN</name>
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        <submittedName>
            <fullName evidence="2">UbiE/COQ5 methyltransferase</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">Nwi_1896</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1896"/>
    <dbReference type="EnsemblBacteria" id="ABA05156">
        <property type="protein sequence ID" value="ABA05156"/>
        <property type="gene ID" value="Nwi_1896"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1896"/>
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    <dbReference type="HOGENOM" id="CLU_037990_2_5_5"/>
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    <dbReference type="OrthoDB" id="1664438at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="1">
        <location>
            <begin position="56"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="285" mass="30737" checksum="27B3FDCD9A41BF8C" modified="2005-10-11" version="1">MAGVPETQNADQIAYWNGPGGQRWADRQRVQDELLEPVSTILLDRARAMPGERIIDVGCGCGATTRAFAEQVGVSGYVLGIDVSAPMLERAGQIAPQAGRVEFRLADAAEHPFRPARFDLIASRFGVMFFAEPASAFANLRKALRPLGRLAFICWREPRFNPWLMAPLQAAYKHVPKLPDLGPEDPGPFSFASEERVHRILGQAGFSEIAMEACELALDISVGGGLDAAVRSALQIGPASRALDGQPAEVIAAAENSIREALAPFAQKQTVVLPASIWIVTARNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRF3</accession>
    <name>Q3SRF3_NITWN</name>
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            <fullName evidence="1">NADH-quinone oxidoreductase subunit J</fullName>
            <ecNumber evidence="1">7.1.1.-</ecNumber>
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        <name evidence="2" type="ordered locus">Nwi_1878</name>
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        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I subunit 6 family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315134.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1878"/>
    <dbReference type="EnsemblBacteria" id="ABA05138">
        <property type="protein sequence ID" value="ABA05138"/>
        <property type="gene ID" value="Nwi_1878"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1878"/>
    <dbReference type="eggNOG" id="COG0839">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085957_5_1_5"/>
    <dbReference type="OMA" id="MLVNKRE"/>
    <dbReference type="OrthoDB" id="1683794at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001457">
        <property type="entry name" value="NADH_UbQ/plastoQ_OxRdtase_su6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042106">
        <property type="entry name" value="Nuo/plastoQ_OxRdtase_6_NuoJ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00499">
        <property type="entry name" value="Oxidored_q3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0520">NAD</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1" id="KW-0874">Quinone</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="212" mass="22996" checksum="E8CFC1C8E4372350" modified="2005-10-11" version="1">MILPALFFYLFAGVCVASAAMVISSRNPVHSVLFLILAFVNAAGLFILMGAEFLAMILIVVYVGAVAVLFLFVIMMLDVDFAELREGFLEYLPFGLVIAAIFLLELLLVVGGWTLNPAVTKSITAAIPGDVSNTEALGLVLYTKYIHYFQLAGMVLLVAMIGAIVLTLRHRAGVKRQDINVQNARTPEMAMSVRQVASGQGLQDADQAEWVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRK1</accession>
    <name>Q3SRK1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_1830</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
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                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315086.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1830"/>
    <dbReference type="EnsemblBacteria" id="ABA05090">
        <property type="protein sequence ID" value="ABA05090"/>
        <property type="gene ID" value="Nwi_1830"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1830"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_61_5_5"/>
    <dbReference type="OMA" id="AKGAWNG"/>
    <dbReference type="OrthoDB" id="1384345at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="83"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="169"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="223"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="253"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="278"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="350"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="376"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="17"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="398" mass="41129" checksum="DC5D8B9E1F445C35" modified="2005-10-11" version="1">MTSADPAAAGKPKLPPLLNIIALATFAASLSTRAMDPVLPHVAEDLSVTVATAAGLSAVTAFSFAIVQPLIGAAADIFGKGRLMLVCLVLLGISNVAGALCTSYAALFVTRIFAGIASGGVFPVALGMASDLVPVPQRQVAIGRTIAGTMGGNLLGASFAGVIGDLVGWRGVLVVLGALVLAVSIAVGLGFQRGDAIATSRRMKLTDLRQGYRQIFSNSNARVCYSAVFIEGCCVLGLFPYVAAFLFELGETRLSIAGIVIAGFAVGGLFYTMTVSRFLVLLGVRGMMISGALLVAAQIVAVGLGPDWRIQLLSFVAMGWGFYMLHGCLQVFASQLSEQARATALSLHSFFFYMGQTAGPVAYGLSLSWVGKFPTLAVNALVIAILGLVCARLLAAKA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-16" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRQ5</accession>
    <name>SURE_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">5'-nucleotidase SurE</fullName>
            <ecNumber evidence="1">3.1.3.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Nucleoside 5'-monophosphate phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">surE</name>
        <name type="ordered locus">Nwi_1775</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Nucleotidase that shows phosphatase activity on nucleoside 5'-monophosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:12484"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18254"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
            <dbReference type="EC" id="3.1.3.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="1">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SurE nucleotidase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.5" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315032.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRQ5"/>
    <dbReference type="SMR" id="Q3SRQ5"/>
    <dbReference type="STRING" id="323098.Nwi_1775"/>
    <dbReference type="EnsemblBacteria" id="ABA05036">
        <property type="protein sequence ID" value="ABA05036"/>
        <property type="gene ID" value="Nwi_1775"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1775"/>
    <dbReference type="eggNOG" id="COG0496">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045192_1_2_5"/>
    <dbReference type="OMA" id="DCVHIAL"/>
    <dbReference type="OrthoDB" id="1909278at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106411">
        <property type="term" value="F:XMP 5'-nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1210.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00060">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030048">
        <property type="entry name" value="SurE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002828">
        <property type="entry name" value="SurE-like_Pase/nucleotidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036523">
        <property type="entry name" value="SurE-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30457">
        <property type="entry name" value="PTHR30457"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01975">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64167">
        <property type="entry name" value="SSF64167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00087">
        <property type="entry name" value="surE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000235628" description="5'-nucleotidase SurE">
        <location>
            <begin position="1"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00060"/>
        </source>
    </evidence>
    <sequence length="255" mass="27636" checksum="3BAB9B599E7F1239" modified="2005-10-11" version="1">MRILCTNDDGIYAPGLEIIEQIAKDLSDDVWVVAPEHDQSGVSHSLTLNDPLRLRQIGPRHFAVKGTPTDCVIMGSRYILADKAPDLVLSGVNRGRNLAEDVVYSGTVAGALEGTMLGLPSFALSQEFSMETGDRPVWETARTFAPQILRRVIEIGIPKNTVVNVNFPACAPEDVAGVLVTRMGKRNLGFLKIDERRDGRGNPYFWIGFEKADDADTPASGSDLAAIAGQCVSVTPLRLDRTDEAFAAILTAKLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-09-02" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRR6</accession>
    <name>TRMFO_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO</fullName>
            <ecNumber evidence="1">2.1.1.74</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Folate-dependent tRNA (uracil-5-)-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Folate-dependent tRNA(M-5-U54)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trmFO</name>
        <name type="ordered locus">Nwi_1764</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H(+) + NADH + uridine(54) in tRNA = (6S)-5,6,7,8-tetrahydrofolate + 5-methyluridine(54) in tRNA + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:16873"/>
            <dbReference type="Rhea" id="RHEA-COMP:10167"/>
            <dbReference type="Rhea" id="RHEA-COMP:10193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74447"/>
            <dbReference type="EC" id="2.1.1.74"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H(+) + NADPH + uridine(54) in tRNA = (6S)-5,6,7,8-tetrahydrofolate + 5-methyluridine(54) in tRNA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:62372"/>
            <dbReference type="Rhea" id="RHEA-COMP:10167"/>
            <dbReference type="Rhea" id="RHEA-COMP:10193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74447"/>
            <dbReference type="EC" id="2.1.1.74"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MnmG family. TrmFO subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.74" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315021.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRR6"/>
    <dbReference type="SMR" id="Q3SRR6"/>
    <dbReference type="STRING" id="323098.Nwi_1764"/>
    <dbReference type="PRIDE" id="Q3SRR6"/>
    <dbReference type="EnsemblBacteria" id="ABA05025">
        <property type="protein sequence ID" value="ABA05025"/>
        <property type="gene ID" value="Nwi_1764"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1764"/>
    <dbReference type="eggNOG" id="COG1206">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033057_1_0_5"/>
    <dbReference type="OMA" id="MHRNTFL"/>
    <dbReference type="OrthoDB" id="516534at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030698">
        <property type="term" value="F:5,10-methylenetetrahydrofolate-dependent tRNA (m5U54) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047151">
        <property type="term" value="F:methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01037">
        <property type="entry name" value="TrmFO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002218">
        <property type="entry name" value="MnmG-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040131">
        <property type="entry name" value="MnmG_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004417">
        <property type="entry name" value="TrmFO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806">
        <property type="entry name" value="PTHR11806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806:SF2">
        <property type="entry name" value="PTHR11806:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01134">
        <property type="entry name" value="GIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00137">
        <property type="entry name" value="gid_trmFO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000346368" description="Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO">
        <location>
            <begin position="1"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="15"/>
            <end position="20"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01037"/>
        </source>
    </evidence>
    <sequence length="477" mass="51182" checksum="BF0F320B3FDB0EE2" modified="2005-10-11" version="1">MTQFESSRATVHIVGAGLAGSEAAWQVAAQGVRVVLHEMRPHRMTAAHRTGGLAELVCSNSFRSDDASNNAVGLLHAEMRRLGSLVMRCADANQVPAGGALAVDRDGFSAAVTEALDNHPLIEIDRAEIDGLPPADWGNVIVATGPLTSTPLAAAIRALTDESALAFFDAIAPIVHRDSIDMSKAWFQSRYDKAGPGGSGADYINCPLSEAQYHAFVDALLEGEKVDFKDWETAPPYFDGCLPIEVMAERGRETLRYGPMKPVGLTNPHDPATKPYAVVQLRQDNKLGTLYNIVGFQTKLKHGAQTRIFRTIPGLEGAEFARLGGLHRNTFLNSPKLLDVRLRLRAEPRLRFAGQMTGCEGYVESAAIGLLGGLYAAADARSCTLEAPPQTTALGALLGHITGGHIETIDTGPRSFQPMNINFGLFPPLADPPTRKPDGTRLRGNEKTIAKKQAICARALSDLDRWIADALRPAAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRS4</accession>
    <name>Q3SRS4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Signal transduction histidine kinase</fullName>
            <ecNumber evidence="4">2.7.13.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1756</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.13.3" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315013.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1756"/>
    <dbReference type="EnsemblBacteria" id="ABA05017">
        <property type="protein sequence ID" value="ABA05017"/>
        <property type="gene ID" value="Nwi_1756"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1756"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3920">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_57_5"/>
    <dbReference type="OMA" id="DQYMPGL"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004673">
        <property type="term" value="F:protein histidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011495">
        <property type="entry name" value="Sig_transdc_His_kin_sub2_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13581">
        <property type="entry name" value="HATPase_c_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07568">
        <property type="entry name" value="HisKA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="6"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="2">
        <location>
            <begin position="159"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="362" mass="39016" checksum="A668191533FB50FB" modified="2005-10-11" version="1">MNVKPTLLYVDDDKALARLVERGLTRHGFCVEYASDGEAGLARIAQGGIDVIALDQYMPGLDGLETLGRIQAIPNAPPVVFVTASQNSKIAVTALKAGAADYLVKDTLGEFVPLLHVAAEVAIRQALVQKARDEAEAEVRASRDRYAALAAEREMLLREVNHRIGNSLQIIASLLHLQANSSPEEDIKAALTNAMGRVAAVAQVHRRLYTSHDLKSVLLNQYLEALLEDLRRSTEGNRMSRLDLKAQPVEIDPDRAVAIGIIVNELVMNAVKYAYPDGPGPIHVELASDGDDIVLSITDDGVGLNARSDPRSTGMGQRIVSAMAQKLDAQAERDPTHAGTRIVLRFRSGTPQVAKPMSATAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRT0</accession>
    <name>Q3SRT0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DSBA oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1750</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315007.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1750"/>
    <dbReference type="EnsemblBacteria" id="ABA05011">
        <property type="protein sequence ID" value="ABA05011"/>
        <property type="gene ID" value="Nwi_1750"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1750"/>
    <dbReference type="eggNOG" id="COG1651">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_47_4_5"/>
    <dbReference type="OMA" id="CKKIEPS"/>
    <dbReference type="OrthoDB" id="1805428at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001853">
        <property type="entry name" value="DSBA-like_thioredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041205">
        <property type="entry name" value="ScsC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01323">
        <property type="entry name" value="DSBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18312">
        <property type="entry name" value="ScsC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229230" evidence="1">
        <location>
            <begin position="25"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="16"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="254" mass="27420" checksum="5DA58F28BE960A98" modified="2005-10-11" version="1">MSSLRFLAPALLALAFSAPSAASAQSFSDSQRGEIEQIVKHYLLAHPEILEEVSAELSKRQAAAEALKHEAAVTENANAIFNSPRGVTLGNKNGDVTFVEFFDYNCGYCKRAMLDMLELMKSDPKLKVVLKEFPVLGQSSVEAAQVAVAARMQDPTGKKYLDFHQKLLGSRGQADKARALAAAKEAGFDMARIEKDMTSAEVRATLEENFKLAESMGMNGTPSYVIGKQVVVGAVGLENLKEKINTARCGKASC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRT1</accession>
    <name>Q3SRT1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Peptidase M48, Ste24p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_1749</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1749"/>
    <dbReference type="EnsemblBacteria" id="ABA05010">
        <property type="protein sequence ID" value="ABA05010"/>
        <property type="gene ID" value="Nwi_1749"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1749"/>
    <dbReference type="eggNOG" id="COG4783">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030556_2_0_5"/>
    <dbReference type="OMA" id="HLSQRHF"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229178" evidence="7">
        <location>
            <begin position="45"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M48" evidence="8">
        <location>
            <begin position="62"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="477" mass="52153" checksum="ED5652296791504C" modified="2005-10-11" version="1">MIPKALTNEDMTRRPVTPLRRSVSKLMTLVTACAIANASFPAQAQQDKGLPMLRDSETEQLLRDYTRPILRAAGLQKHNIQMVIINDRSFNAFVADGRRIFVNYGAISKSTTPNQLIGVLAHETGHLAGGHLSKFREQLARTQTQLIIAMLLGAGALAAGAGRGGSNSPGSNIGAAALSAPQEIARRSLLGYQRQQEENADRAAVKFLNATGQSPRGMYETFKRFTDESLFAARGADPYAQSHPMPADRVAALAELVKTSPNWQKKDDPALQMRHDMVRAKISAFMEREDTVYRRYPRSDTSLPARYAHAITTYLHGDLRSAIEQIDALIKLQPQNPYFYEVRGQALLEGGRPTEAIAPLRRAVQLSRNAPLIEMLLGQALVASNNKANADEAIAILRTALVREPEAPLGYSQLAIAYGRKGDYAQADLASAQAAFLRGDNKTARELASRAKTRFAIGTPGWVKADDIVSSKIPDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRT8</accession>
    <name>Q3SRT8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1742</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314999.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1742"/>
    <dbReference type="EnsemblBacteria" id="ABA05003">
        <property type="protein sequence ID" value="ABA05003"/>
        <property type="gene ID" value="Nwi_1742"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1742"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_601083_0_0_5"/>
    <dbReference type="OMA" id="YSIDMAF"/>
    <dbReference type="OrthoDB" id="720059at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229233" evidence="2">
        <location>
            <begin position="26"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="95"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="451" mass="50027" checksum="FDE6A7769CF1FF6B" modified="2005-10-11" version="1">MKLIQTAKVASVLLTMTACFSGAAAQSTTSPISASLISAASAVQQDYDALFQQMYKNPADLEASFKFAEQAVKRGDYEAAIGALERMLFFNPNLPRVKLELGVLYFKLGSYELARSYFQEAIKGADAPDDIRAQVRAYLTEIDRRLARYEFSVFTTAGLRYQTNANLGPSSLMVRALGQDAILNSSFGKSPDVNMFQTLAAHYAYKIGTRGDAIEASFLGLNSRQYKLNQFNFGLVEVVVGPRFAIGQNASVKLYGIGDQVWLGDATYFSAGGAGFSARTTIGDRGFLEAFVEQRHRNFSDSFNFPTASQQTGDLLSAVVTSDLRLGPMHWTTRAAFDQNRAAFDFYSYKRYSIDMAFPYAFALPVFGTPHQFVIAPTFGFSRANYDAPNPIVDPFQIRHDWEQRYGAIFDAQVYRNVGLRTQILYTKIDSSLPNFTLDNFSVSIGPTARF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRV7</accession>
    <name>RISB_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">6,7-dimethyl-8-ribityllumazine synthase</fullName>
            <shortName evidence="1">DMRL synthase</shortName>
            <shortName evidence="1">LS</shortName>
            <shortName evidence="1">Lumazine synthase</shortName>
            <ecNumber evidence="1">2.5.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ribH</name>
        <name type="ordered locus">Nwi_1723</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + H(+) + 2 H2O + phosphate</text>
            <dbReference type="Rhea" id="RHEA:26152"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58201"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="2.5.1.78"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DMRL synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.78" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314980.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRV7"/>
    <dbReference type="SMR" id="Q3SRV7"/>
    <dbReference type="STRING" id="323098.Nwi_1723"/>
    <dbReference type="EnsemblBacteria" id="ABA04984">
        <property type="protein sequence ID" value="ABA04984"/>
        <property type="gene ID" value="Nwi_1723"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1723"/>
    <dbReference type="eggNOG" id="COG0054">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089358_1_2_5"/>
    <dbReference type="OMA" id="CQGVTQG"/>
    <dbReference type="OrthoDB" id="1680292at2"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00404"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009349">
        <property type="term" value="C:riboflavin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000906">
        <property type="term" value="F:6,7-dimethyl-8-ribityllumazine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09209">
        <property type="entry name" value="Lumazine_synthase-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00178">
        <property type="entry name" value="Lumazine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034964">
        <property type="entry name" value="LS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002180">
        <property type="entry name" value="LS/RS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036467">
        <property type="entry name" value="LS/RS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21058">
        <property type="entry name" value="PTHR21058"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00885">
        <property type="entry name" value="DMRL_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52121">
        <property type="entry name" value="SSF52121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00114">
        <property type="entry name" value="lumazine-synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000040467" description="6,7-dimethyl-8-ribityllumazine synthase">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="58"/>
            <end position="60"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="87"/>
            <end position="89"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="92"/>
            <end position="93"/>
        </location>
        <ligand>
            <name>(2S)-2-hydroxy-3-oxobutyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>(2S)-2-hydroxy-3-oxobutyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00178"/>
        </source>
    </evidence>
    <sequence length="163" mass="17284" checksum="9ED52FB73390F084" modified="2005-10-11" version="1">MAEARHAPLKDQTSVSGARVLIVEARFYDDIQDALLEGAVAELQSAGVRHDVVTVPGALEIPAAIVFALDAAERRGEPYDAVVALGCVVRGDTIHFEIVSMESARALMDLSVKRGVALGNGIITVDTDAQAWARARAGELNKGGHAAQAALTMLRMKRRLAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRW9</accession>
    <name>Q3SRW9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1711</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314970.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1711"/>
    <dbReference type="EnsemblBacteria" id="ABA04972">
        <property type="protein sequence ID" value="ABA04972"/>
        <property type="gene ID" value="Nwi_1711"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1711"/>
    <dbReference type="eggNOG" id="ENOG50316B4">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_193824_0_0_5"/>
    <dbReference type="OMA" id="QAHRLDM"/>
    <dbReference type="OrthoDB" id="2012908at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="77" mass="8429" checksum="1E120D3D443E6D28" modified="2005-10-11" version="1">MRERPNRNEELRDDESCLGDPPGDGGPDEAAHYLAGAVADLALIARRHGLDTLGYLLDMAQLEAEEIVRLRTARKGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRX7</accession>
    <name>Q3SRX7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized conserved protein UCP032025</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1703</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314962.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1703"/>
    <dbReference type="EnsemblBacteria" id="ABA04964">
        <property type="protein sequence ID" value="ABA04964"/>
        <property type="gene ID" value="Nwi_1703"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1703"/>
    <dbReference type="eggNOG" id="COG5458">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130421_0_0_5"/>
    <dbReference type="OMA" id="PMRPFQG"/>
    <dbReference type="OrthoDB" id="1751241at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008320">
        <property type="entry name" value="UCP032025"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07370">
        <property type="entry name" value="DUF1489"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032025">
        <property type="entry name" value="UCP032025"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="154" mass="17325" checksum="0EDDDA9E0FEA5CF2" modified="2005-10-11" version="1">MIHSTAAADRHLHLIKLAVGCESVKELRDWIAERMKQATLEGLPRHHIHITRMTPKRGEELLAGGSLYWVIRGEIAAREKLIGIEPFRDGDGIGRCRLVMQPKVVAVRPRPMRPFQGWRYLSLADAPPDLGSNAASVAEMPEPLRRELRDLGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRZ1</accession>
    <name>Q3SRZ1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1689</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314948.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04950">
        <property type="protein sequence ID" value="ABA04950"/>
        <property type="gene ID" value="Nwi_1689"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1689"/>
    <dbReference type="HOGENOM" id="CLU_2480207_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="87" mass="9553" checksum="9829CA973F034541" modified="2005-10-11" version="1">MCALEDLFLILCLLSVFLLALYGVRGWLLLINKEAESACPRCGMAMTMDKSVAVARLVRRCPHCETEADPLQASASGWIKSSLKPPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS42</accession>
    <name>Q3SS42_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphoesterase, PA-phosphatase related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1638</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1638"/>
    <dbReference type="EnsemblBacteria" id="ABA04899">
        <property type="protein sequence ID" value="ABA04899"/>
        <property type="gene ID" value="Nwi_1638"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1638"/>
    <dbReference type="eggNOG" id="COG0671">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1029858_0_0_5"/>
    <dbReference type="OMA" id="REWRREM"/>
    <dbReference type="OrthoDB" id="1930882at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxi_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="SSF48317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="acidPPc" evidence="2">
        <location>
            <begin position="109"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="270" mass="30118" checksum="1B40E0FD36ED3510" modified="2005-10-11" version="1">MRISDRPTIIVVDRHPFARKSTVAKIAALRSDFHVRLHAAKSTTMTITGLDREILLTLNAFGGGNDTLWEFANNSLFRGFPVFFSLVALWFATEQPERRGRMLAGLLGVCLATMLSIWSQFHTAVHLRPILDPSIPLDTVIPRTPWDRTNSFPSDTATLFFSLAALVFVEKRSAGLFCFAWVALIVAIPRVIVGLHYPSDILGSFVLGPAVVFILAALPYPKRLFEHALTWFEGRLYIVHALLFIFLADAFDLFPSLQALGKVLARSLHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS59</accession>
    <name>Q3SS59_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phage protein, HK97, gp10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1477</name>
        <name evidence="2" type="ordered locus">Nwi_1621</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="2">Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314745.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1477"/>
    <dbReference type="EnsemblBacteria" id="ABA04738">
        <property type="protein sequence ID" value="ABA04738"/>
        <property type="gene ID" value="Nwi_1477"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04882">
        <property type="protein sequence ID" value="ABA04882"/>
        <property type="gene ID" value="Nwi_1621"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1477"/>
    <dbReference type="KEGG" id="nwi:Nwi_1621"/>
    <dbReference type="eggNOG" id="ENOG50316U7">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127674_2_1_5"/>
    <dbReference type="OMA" id="HLVEYGT"/>
    <dbReference type="OrthoDB" id="1821542at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010064">
        <property type="entry name" value="HK97-gp10_tail"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04883">
        <property type="entry name" value="HK97-gp10_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01725">
        <property type="entry name" value="phge_HK97_gp10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="141" mass="15208" checksum="9CA24A03255583B3" modified="2005-10-11" version="1">MTNDDGGLSSFQRRMAAIPKEVRAAVKPALLKSGEELKSAMRQLAEASRDTGDLIDSIAVTGPGETTPAYSQPGGSKVVGETSVAVTVGNTKVRYAHLVEYGTAKAPAQPFFWPAFRLYRKRITSRIKRAINKAVKESKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSB0</accession>
    <name>Q3SSB0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1570</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04831">
        <property type="protein sequence ID" value="ABA04831"/>
        <property type="gene ID" value="Nwi_1570"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1570"/>
    <dbReference type="HOGENOM" id="CLU_2451639_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="89" mass="9875" checksum="FD7C84440C9E5358" modified="2005-10-11" version="1">MKKTRPIKIIEPASDFIRSGKALKIKIGLLHPPVEAADGGMMSASEPWHQEPWHQERWASGARKAGSKLAQMGMRGVESKTASRLKVRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSG5</accession>
    <name>Q3SSG5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATP-dependent DNA helicase RecG</fullName>
            <ecNumber evidence="2">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_1515</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecG subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314782.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1515"/>
    <dbReference type="EnsemblBacteria" id="ABA04776">
        <property type="protein sequence ID" value="ABA04776"/>
        <property type="gene ID" value="Nwi_1515"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1515"/>
    <dbReference type="eggNOG" id="COG1200">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005122_7_1_5"/>
    <dbReference type="OMA" id="DNGFQAC"/>
    <dbReference type="OrthoDB" id="234717at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045562">
        <property type="entry name" value="RecG_dom3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033454">
        <property type="entry name" value="RecG_wedge"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19833">
        <property type="entry name" value="RecG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17191">
        <property type="entry name" value="RecG_wedge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="4">
        <location>
            <begin position="286"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="5">
        <location>
            <begin position="466"/>
            <end position="625"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA04776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="699" mass="76424" checksum="434FB0DCDA410D1F" modified="2005-10-11" version="1">MRPALLNPLFVPVTSLSGVGPKQDRLFRHLLARDETPRLVDLLFHLPASVVDRRARPKIRDAVPGTIVTLEVSVDRHRPAPPGRSRAPHLVYASDETGSVVLTYFRAQPSYVEKLLPAGSKRYVSGTVQMFDGTLQIVHPDRVVDEAGLAELSGIDPVYPLTQGLAPGALRRAIAQALQNLPVLPEWTDPKILRRCGFPPLAQALRRVHVPLELTDTLPEGPFWSRLAFDELLAGQLALTLVRARLRRPAGNRHAGDGHLRNRIIDALPYALTASQQQAVAAIIEDLRQPVRMLRLLQGDVGSGKTVVALLAACAVAESGKQTALMAPTEVLARQHIKTIAPLAERAGLRVAILTGREKGRERREITARLAEGDIDLLVGTHALIQDEVTFKSLALAVVDEQHRFGVRERLALTTKGEAVDVLVLSATPIPRTLVLTYFGDMDVSELRDKPAGRQPIDTRAVSDGRLTEVIDAVGRAVKAGKLAYWICPLVEESDAVDLSDAEQRYQSLKARFGDRVGLVHGKMRGPEKDRVMAQFAAGEINLLVATTVVEVGVDVPAATIMVIENAERFGLAQLHQLRGRIGRGSEPSTCLLLYREPLGEMSAARLRVIRETTDGFRIAEEDLRLRGEGDVLGTRQSGLPGYRVARSDVHAQLITLARDEALRILKQNPNLTGSHGEALRCLLYLFERDEALPLLGAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSK8</accession>
    <name>Q3SSK8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phage Terminase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1472</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314740.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1472"/>
    <dbReference type="EnsemblBacteria" id="ABA04733">
        <property type="protein sequence ID" value="ABA04733"/>
        <property type="gene ID" value="Nwi_1472"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1472"/>
    <dbReference type="eggNOG" id="COG4626">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026632_6_0_5"/>
    <dbReference type="OMA" id="CGIIDEY"/>
    <dbReference type="OrthoDB" id="1527109at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005021">
        <property type="entry name" value="Terminase_largesu_Lambdalike"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41287">
        <property type="entry name" value="PTHR41287"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03354">
        <property type="entry name" value="Terminase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="537" mass="60463" checksum="A0EE30C06CBDFB86" modified="2005-10-11" version="1">MLVPAWINDGSEIPDPFGYGERAVQWLRRLKHPKNPARGHPFQLDPWQERIIRKLYGPRHEDGRRVVRRLVLLLPRGNRKTSLCGAITLLHLIGPERQPGNLVVSAASAHEQAMELFNEVAMIVQHDRRLEKHLAVREYTSSITFKAQRSRYIAVASDGKVQHGKTPNVVIADELHAWEGRAGRVQWEALDSALVKVPDTLMIVATTSGRGQENLAWKTIDYAIKVQKGEIDDPATLPVVFMAEEGDDWRDEAVWHALNPGMRHGYPDLTSYRDKAQKAINSPSDRDSFLQFNLNRWLDHSTSPFVEMLVYDQGARAVNLDELKGESCWIGVDLSSNSDLTAVVACWRDGDDGYQVQPWFFCPEDNLRRRADKDGVAYPAWAADGFIIPTPGNVVDFHVVENHIRDLCDRFNVREIAFDPHLARNMLSNLLADGLPAVEMRQGWVTMAPAIKELERAIVGRRFRHGGHPVLRWNFDNIATETDKAGNKSFHKGKSRDRIDGAVATAMAVARCAAGDNARSIYDSPNWSPDWAFVKAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSL3</accession>
    <name>Q3SSL3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PilZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1467</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314735.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04728">
        <property type="protein sequence ID" value="ABA04728"/>
        <property type="gene ID" value="Nwi_1467"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1467"/>
    <dbReference type="eggNOG" id="ENOG50313D7">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158569_2_0_5"/>
    <dbReference type="OrthoDB" id="1800377at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035438">
        <property type="term" value="F:cyclic-di-GMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009875">
        <property type="entry name" value="PilZ_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07238">
        <property type="entry name" value="PilZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PilZ" evidence="1">
        <location>
            <begin position="7"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="98" mass="10916" checksum="30D66FEFCE8C255A" modified="2005-10-11" version="1">MNSPTEDRRKHPRVEIDEPAYISMGGFSMRCRLLNVSPEGAALEVPNPASVPLSFQLMTEKDRVIRGCRVIWTKQNRIGVAFDQPTAEAGFDAPSRNS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-04-03" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSM1</accession>
    <name>Y1459_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0060 membrane protein Nwi_1459</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">Nwi_1459</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0060 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314727.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSM1"/>
    <dbReference type="STRING" id="323098.Nwi_1459"/>
    <dbReference type="EnsemblBacteria" id="ABA04720">
        <property type="protein sequence ID" value="ABA04720"/>
        <property type="gene ID" value="Nwi_1459"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1459"/>
    <dbReference type="eggNOG" id="COG1742">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117653_1_0_5"/>
    <dbReference type="OMA" id="WLWAVEG"/>
    <dbReference type="OrthoDB" id="1991345at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00010">
        <property type="entry name" value="UPF0060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003844">
        <property type="entry name" value="UPF0060"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36116">
        <property type="entry name" value="PTHR36116"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02694">
        <property type="entry name" value="UPF0060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000282240" description="UPF0060 membrane protein Nwi_1459">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00010"/>
        </source>
    </evidence>
    <sequence length="108" mass="11559" checksum="E8D091201BFE7369" modified="2005-10-11" version="1">MITPAAYVGAAIAEIAGCFAFWAWLRLGKSVWWLVPGMASLALFAYLLTLVDSEAAGRAYAAYGGIYIMASLGWLWSVEGIEPDRWDLAGALICLIGAVVILIGPHEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSR8</accession>
    <name>Q3SSR8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Sugar transferase</fullName>
            <ecNumber evidence="11">2.7.8.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_1412</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial sugar transferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.6" evidence="11"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314682.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1412"/>
    <dbReference type="EnsemblBacteria" id="ABA04673">
        <property type="protein sequence ID" value="ABA04673"/>
        <property type="gene ID" value="Nwi_1412"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1412"/>
    <dbReference type="eggNOG" id="COG2148">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024920_0_1_5"/>
    <dbReference type="OMA" id="NWSVLFD"/>
    <dbReference type="OrthoDB" id="1759731at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047360">
        <property type="term" value="F:undecaprenyl-phosphate galactose phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003362">
        <property type="entry name" value="Bact_transf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017475">
        <property type="entry name" value="EPS_sugar_tfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017473">
        <property type="entry name" value="Undecaprenyl-P_gluc_Ptfrase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02397">
        <property type="entry name" value="Bac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03025">
        <property type="entry name" value="EPS_sugtrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03023">
        <property type="entry name" value="WcaJ_sugtrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0270">Exopolysaccharide synthesis</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="321"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="Bac_transf" evidence="10">
        <location>
            <begin position="315"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA04673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="510" mass="57461" checksum="1334997B8921C981" modified="2005-10-11" version="1">MDPINARSRTDAAASSSPVVPVERRRRLSPAALAVTNQKVQPAYSPVVIMGIVRAADFLLLSLVGVALYLGYVARIDGFSLSYIAAILAVSASAVVCFQAADIYDIQVFRGPLRQMTRMISSWAFVFLLFICVSFFVKLGNSVSRLWLASFFFVGLAGLIAGRVLLRAMIRRWARDGRLERRTVIIGSDKNGENLINALKAQEDSDIEILGVFDDRNDSRALDTCAGTPKLGKVDDVLEFARRTRLDLVLFALPISAETRILSMLKKLWVLPVDIRLSAHTNQLRFRPRAYSYLGKVPTIDVVEAPITDWDLVMKWLFDRIFGGVILLLASPVMALVALAIKLDSPGPVLFRQKRFGFNNERIEVFKFRSLFHDQADPMAAKVVTKNDSRVTRVGRFIRKTSLDELPQLFNVVFKGNLSLVGPRPHAVQGKLQTRLFDEAVDGYFARHRVKPGITGWAQINGWRGEIDNEEKIQKRVEFDLYYIENWSVLFDLYILFRTPLALLKSENAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSZ0</accession>
    <name>Q3SSZ0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fec I like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1340</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314619.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1340"/>
    <dbReference type="EnsemblBacteria" id="ABA04601">
        <property type="protein sequence ID" value="ABA04601"/>
        <property type="gene ID" value="Nwi_1340"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1340"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_12_5_5"/>
    <dbReference type="OMA" id="MTEASWT"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="2">
        <location>
            <begin position="27"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="3">
        <location>
            <begin position="127"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="186" mass="20929" checksum="27233A11F18958F9" modified="2005-10-11" version="1">MPAYSDRNRSVAPASILDSDNLLDRAIEAHYADIKAAVLRRGCARGLVSDVVHDLYIKLAEQPDLLKNKRSLCAFLCRSAINLGIDRMRRARLEQHLFSGTEAEALAVPAETAAPDYGLDVEARLAVLREAITELPARQRAVFILHRLHRLEPDQIARKLNISRNMVDRHLRRALAHCLDRLLGIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST19</accession>
    <name>Q3ST19_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1311</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314592.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1311"/>
    <dbReference type="EnsemblBacteria" id="ABA04572">
        <property type="protein sequence ID" value="ABA04572"/>
        <property type="gene ID" value="Nwi_1311"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1311"/>
    <dbReference type="eggNOG" id="COG1262">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012431_9_0_5"/>
    <dbReference type="OMA" id="CRGTFRN"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052699">
        <property type="term" value="P:ergothioneine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1580.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024775">
        <property type="entry name" value="DinB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034660">
        <property type="entry name" value="DinB/YfiT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017806">
        <property type="entry name" value="EgtB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005532">
        <property type="entry name" value="SUMF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042095">
        <property type="entry name" value="SUMF_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12867">
        <property type="entry name" value="DinB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03781">
        <property type="entry name" value="FGE-sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109854">
        <property type="entry name" value="SSF109854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="SSF56436"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03440">
        <property type="entry name" value="egtB_TIGR03440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DinB_2" evidence="2">
        <location>
            <begin position="31"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="FGE-sulfatase" evidence="1">
        <location>
            <begin position="197"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="415" mass="46701" checksum="4BBB6FD814518FBE" modified="2005-10-11" version="1">MANPASAAGAVPRVSPFAASRGLADDVVEAFLAVRSETERRAKPLTPEDQMVQSMPDASPVKWHRAHTTWFWEQFLLARHSPGYVGFHPDYAFLFNSYYVSAGPRHARAERGLLTRPGAEEITAYRRHVDAAVVRFLKEADDDALAEMMPLIEAGLNHEQQHQELMLTDILHAFAQNPIAPAYDTSWPFSGPHTGSGWITLNQGIHTIGHQGDSFHFDNEKPAHRVLVGPVKLARGLVTNADWLAFMDDGGYRDATLWLMDGFAAATSEAWEAPAYWRRKDDGWQVMTLGGLRPLDPNAPVRHVSYYEADAFARWSGKHLPTEMEWEVAARAGHLDDAFGVAWQWTRSAYAPYPGYRASRDTLGEYNGKFMVNQFVLRGSSLATPARHSRVSYRNFFYPHQRWQFTGLRLADYGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST56</accession>
    <name>Q3ST56_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Peptidase C14, caspase catalytic subunit p20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1274</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1274"/>
    <dbReference type="EnsemblBacteria" id="ABA04535">
        <property type="protein sequence ID" value="ABA04535"/>
        <property type="gene ID" value="Nwi_1274"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1274"/>
    <dbReference type="eggNOG" id="COG4249">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_528801_0_0_5"/>
    <dbReference type="OMA" id="LMFYAGH"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029030">
        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001309">
        <property type="entry name" value="Pept_C14_p20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015917">
        <property type="entry name" value="Pept_C14A"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00376">
        <property type="entry name" value="IL1BCENZYME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="SSF52129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50208">
        <property type="entry name" value="CASPASE_P20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229175" evidence="2">
        <location>
            <begin position="27"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="CASPASE_P20" evidence="3">
        <location>
            <begin position="27"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="379"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="395"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="432"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="485" mass="51661" checksum="C3541720416AB08E" modified="2005-10-11" version="1">MTFSMIRSIVLGVVAAIVLTAHPAAAENRLALVIGQSAYRAVTPLPNPGNDAKAMTQMLGDAGFDVLTASDLSQTEMREKVGEFAARIAEKGPDTIALVFYAGHGLQVDGENFLVPVDLDPKREADIPLQAIRLNDIMNTLTSVPSRMRIVLLDACRNNPFPAINKTVGHGLAIVDAKVAAPGTFLSFSTSPGAEAEDGAGTDSPYTAALLTAGRQPGLSIEEAFKRVRLEVNKATNGRQTPWESSSLTDSFHFFPGSAETGATPPAGSTVKRTVAEWRRELKGKTAEQAHELIVSDGSGQAYEAFVGLYADSPFDLEARQWLDRQRRMQAWNRAVLTNTAAGYRAFLAEYSDSDLTPTARKLEERLRNRPDNPGVAVAAAAAGPTCPCSTPPREPQKKKAETPRKKQAKQDRSRNEDTPPRRGQRRPAYYADDVPPPPPPPSVVPMPLPIPSIGIGGFGGGFGRGGGYGGSGRGGGMGGSTGRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST75</accession>
    <name>Q3ST75_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DEAD/DEAH box helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1255</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314544.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1255"/>
    <dbReference type="REBASE" id="11320">
        <property type="entry name" value="NwiORF1254P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04516">
        <property type="protein sequence ID" value="ABA04516"/>
        <property type="gene ID" value="Nwi_1255"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1255"/>
    <dbReference type="eggNOG" id="COG4096">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018958_0_0_5"/>
    <dbReference type="OMA" id="AGWDEMA"/>
    <dbReference type="OrthoDB" id="16977at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006304">
        <property type="term" value="P:DNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013670">
        <property type="entry name" value="EcoEI_R_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029464">
        <property type="entry name" value="HSDR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08463">
        <property type="entry name" value="EcoEI_R_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13588">
        <property type="entry name" value="HSDR_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="2">
        <location>
            <begin position="179"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="570"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="811" mass="91144" checksum="66738B6FBC34610D" modified="2005-10-11" version="1">MDKRSLTERDICTKFILPAVKRAGWDEMAQVREEVYFTKGRIIVRGKLVTRGKAKKADFVLYYKPNIPIALIEAKDNNHSVGHGIQQGLDYAMTLDIPFVFSSNGDGFVFHDRTGQSVPVETNLGLDAFPSPTDLWARYRAWKGLDAEAEEIVLQDYFDDGSGKTPRYYQVNAVNAAIEAIAKGRDRVLLVMATGTGKTYTAFQIIWRLWKAGRKKRILFLADRNVLIDQTMVNDFRPFGAAMAKLSTNARTIERQDGTAVDLPLALDTRRRIDTAFEIYLGLYQAITGPEERQKLYKEFSPGFFDLVVIDECHRGSAAEDSAWREILDHFSGATQIGLTATPKETEYVSNTEYFGEPVFTYSLKQGISDGFLAPYKVIKVHIDRDVEGYRPELGQLDRDGNEVEDRIYNTKDFDRNIVLDDRTVLTAKKITEFLKESGDRFQKTIVFCVDEEHAARMRQALINENADLVAENQRYVMRITGSDKEGQDQLGNFIDPESKYPVLVTTSRLLSTGVDAQTCRLIVLDRAVGSMTEFKQIVGRGTRVHEDTKKFFFTLIDFRGATSHFADPDFDGDPAQIYEPGSGDPITPPDDAPQGDDTIPPKPGDDETVVDQPGLPLPAGGPIRKIYVDGVGARILAERVEYLDENGKLVTESLRDFTRTALKKRFASLDDFLKRWKSAERKHALIEELEAEGLALDAVAGELGKNLDPFDLICHVAFDRKPLTRRERAENVKKRDVFTKYGPQARAVLDALLEKYRDEGVLNLDDANVLKVTPFTAMGSVVQLIKAFGGKEGFEKAVHEMQDALYQETA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST85</accession>
    <name>Q3ST85_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-ketoacyl synthase</fullName>
            <ecNumber evidence="2">2.3.1.41</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1244</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.41" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314535.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1244"/>
    <dbReference type="EnsemblBacteria" id="ABA04506">
        <property type="protein sequence ID" value="ABA04506"/>
        <property type="gene ID" value="Nwi_1244"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1244"/>
    <dbReference type="eggNOG" id="COG0304">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_69_3_5"/>
    <dbReference type="OMA" id="AWQRIGT"/>
    <dbReference type="OrthoDB" id="606297at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000794">
        <property type="entry name" value="Beta-ketoacyl_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712">
        <property type="entry name" value="PTHR11712"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketoacyl_synth_N" evidence="1">
        <location>
            <begin position="55"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="401" mass="42367" checksum="45F9A0F67053DADB" modified="2005-10-11" version="1">MAETAPPSAPIETWITGIGLATSLGEGLDAHWEAFSHRRVNADEKSFAPYVVHPLAPINFDAQIPKRGDQRQMEAWQRIGTYAAGLALDSAGVKGNTDILSRMDMIVAAGGGERDLVVDAGVLNAETKGNAAPGHLNERLMSDLRPTLFLAQLSNLLAGNIAIVHGVTGSSRTFMGEEASGVDAVRIALARIAAGQSDIALVGAAHNGERKDLLLLYEFGDFNLKEKYAPVWAREHNSGFALGSGGAFLVIESRAHAEARGAKPFARLTNVVADHARRKHSGDVTSTLESLWSRLGNSTEGTAIVTGATGAEPATSEERAFLRAHPDIPVRATGTMFGHTMEAQFPLGLAVAALSLSRGALFASADTSEQAIDMTRPPARIVVIGTGHWRGEGMALVEAIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STA5</accession>
    <name>Q3STA5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phospho-2-dehydro-3-deoxyheptonate aldolase</fullName>
            <ecNumber evidence="3">2.5.1.54</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1224</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>D-erythrose 4-phosphate + H2O + phosphoenolpyruvate = 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14717"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="2.5.1.54"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the class-II DAHP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.54" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314515.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1224"/>
    <dbReference type="EnsemblBacteria" id="ABA04486">
        <property type="protein sequence ID" value="ABA04486"/>
        <property type="gene ID" value="Nwi_1224"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1224"/>
    <dbReference type="eggNOG" id="COG3200">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026885_0_1_5"/>
    <dbReference type="OMA" id="KVMMQMA"/>
    <dbReference type="OrthoDB" id="1371606at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003849">
        <property type="term" value="F:3-deoxy-7-phosphoheptulonate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002480">
        <property type="entry name" value="DAHP_synth_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21337">
        <property type="entry name" value="PTHR21337"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01474">
        <property type="entry name" value="DAHP_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01358">
        <property type="entry name" value="DAHP_synth_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU363071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="462" mass="51054" checksum="E52785DB772DBA07" modified="2005-10-11" version="1">MSERWTPDSWRTKPVQQVPVYPDPKALGDVEAQLATFPPLVFAGEARNLKKALARVANGEAFLLQGGDCAESFAEHGANNIRDFFRVLLQMAVVLTYAGALPVVKVGRIAGQFAKPRSSPMENVGGVELPSYRGDIVNDIAFTEQSRVPDPHRQIMAYRQSAATLNLLRAFATGGFANLGSVHQWMLGFLKDSQQSRRYKELADRISDALNFMRACGLNLESHPELRSTDIYTSHEALLLGYEQALTRVDSTTGDWYATSGHFIWVGDRTRQLDHGHVEYLRGIKNPIGLKCGPSLKPDELLKLIDALNPDNEPGRLTLIGRFGSDKVADYLPQLIRTVKREGRVVVWSCDPMHGNTITSTSGYKTRPFDRILSEVKSFFSIHAAEGTHAGGIHLEMTGQNVTECIGGARAIKDEDLNGRYHTVCDPRLNAEQSIDMAFLIAELLSHDREGKVKPIPAVSGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="129" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STC9</accession>
    <name>Q3STC9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_1200</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314491.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1200"/>
    <dbReference type="EnsemblBacteria" id="ABA04462">
        <property type="protein sequence ID" value="ABA04462"/>
        <property type="gene ID" value="Nwi_1200"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1200"/>
    <dbReference type="eggNOG" id="COG2202">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_71_5"/>
    <dbReference type="OMA" id="GFAFHWQ"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="7">
        <location>
            <begin position="320"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="6">
        <location>
            <begin position="543"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="502"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA04462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="779" mass="85570" checksum="198E5219F01FC783" modified="2005-10-11" version="1">MARAQAASACAQSDSIKGLAQSIAKPAYRRLLIAEPVLRRAVPTLIIAFLVTICIGALVQVIDQTRQKQAMTKHDLLALADLLAERLEHAGVIDQDRPASIERLQNLLPKLIPQWSIATGRHVIVTGVDQRVLARVPVGAASGESSRMLELISITQSPILRGAAPASVADIVLLDGSSVLAVQRVVRSLLGQVTVIQEVNAAWRPDTALSITLSATTGFVVLILGFAFHWQSTRAREGDLINDAVRSRIDTALNRGRCGLWDWDLSRGRIFWSQSMFTMLGMESRSDLLTFGEVSSLVNSDDIDLFEVADQLISGKIGHIDQSFRMRHSSGHWIWLRVRCELSQNANGGAHLIGIAVDVTEQKSLAEKSIEADLRLRDAIESIPEAFVLWDADDRLVLCNSHFQRLHKLPDSAVAVGTPYETVVEVGRMPEIRARHSETGTVTLKAHTFDVQLADGNWLHVSERRTKDGGYVSVGTDITRIKEHEQKLVENDLRLRATVIDLKRTQATLEQQAIKLADLAQKYADEKNRAEDANRAKSKFLANMSHELRTPLNAIIGFSEIMSNGMFGTLGSTKYSEYCRDILTSGQYLLEVINDVLDMSKIEAGRTNLDMETLDLTGVLDESLRVVSGRTSDKGLTFDVRSDDDISIIADRRAVKQIMVNLLSNAVKFTPASGRIMARSKVFENSVTLTIADTGIGIAPHALRRLGKPFEQVESQLTKTHAGSGLGLAIAKSLVNLHSGSMRIRSKPGAGTVVRVTLPRHRQARRRPVPQLTSCLPAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STE7</accession>
    <name>Q3STE7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1182</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314473.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1182"/>
    <dbReference type="EnsemblBacteria" id="ABA04444">
        <property type="protein sequence ID" value="ABA04444"/>
        <property type="gene ID" value="Nwi_1182"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1182"/>
    <dbReference type="eggNOG" id="ENOG50345BZ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028728_0_0_5"/>
    <dbReference type="OrthoDB" id="1612055at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021251">
        <property type="entry name" value="DUF2793"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10983">
        <property type="entry name" value="DUF2793"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="640" mass="65125" checksum="900E9CAD3B066E32" modified="2005-10-11" version="1">MTDTPNLGLPYIDGSQAQKHVTHNEALRILDAAIQIGVLDRTLTTPPSSPAAGERYVVAGGATGDWAGKDDAIATWQDDAWAFLTPKAGWCIWSAADESLFVFDGSAWQGAGGAASLDSVAQLGVNAAASTPNLLTVKSNAALFAAIDAVDGGTGDVRLQVSKEGSANTASVFFSNGFSGRAEFGLVGADAFKLKVSADGSGWIEALDIDQDTGNVALPRGLALTGVVAPAQITADQDDYAPAGLAAASVLQISTDAPRGLSGLAGGTEGRCLIIINVGGQPVTLLNESALSSASNRFSLNDSLVISAKQAAILRYDGTAARWQAIAGGVAGAGASGGGFISPPQGRLTLASGVPVMTTTQSAKSTLYYTPYQGNQIPIYNGTEMVPMAFAELSVATTDTTKNPAAIGAGKVNDWFVWNDGGTLRLSHGPDWTSDTTRSAGTALTRVNGILLNAVAITNGPAASRGTYVGTTRSNGSSLLDWISGSTASGGGMASLNVWNAFNRADVTTTVSDSTGSWSYSSSAVRPSGNSTSNRIQFVSGLAEEGISMMLSQRIATATAAGALGRIGIGLDSTSAYSFHALGVSPASGVVFNAALTVQGATPPQLGAHYLQALESGDGTNATTFVGGGEPYQFSARLRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STH0</accession>
    <name>Q3STH0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1159</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1159"/>
    <dbReference type="EnsemblBacteria" id="ABA04421">
        <property type="protein sequence ID" value="ABA04421"/>
        <property type="gene ID" value="Nwi_1159"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1159"/>
    <dbReference type="eggNOG" id="ENOG503123D">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085713_0_0_5"/>
    <dbReference type="OMA" id="LKPQQRT"/>
    <dbReference type="OrthoDB" id="2033200at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="251" mass="26968" checksum="6CDFB9DA51731891" modified="2005-10-11" version="1">MCATRTGKPARKPAAGRAASRAKLMPSRTKASVASTKAAALSAPDKPLETVSQSRAKKTKAEAATKRSAAGTSPAGKTRAAVAARKATIAATTRYVDDAPVILDENDDAAKAGLSRQRTRRTTVRRKDQPSPRIKRTAVERLDDPPPASGAVLIERVTRAIERELSLIEVIVGGSHLKPQQRTEAERRARTLASLARTLGEVTRLRAGQEKVKPADDDAVPRDLDEFRRALSRRLEQMVAPPATPPAGEHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STK6</accession>
    <name>Q3STK6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Flagellar protein FliL</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_1123</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Controls the rotational direction of flagella during chemotaxis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the FliL family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314416.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1123"/>
    <dbReference type="EnsemblBacteria" id="ABA04385">
        <property type="protein sequence ID" value="ABA04385"/>
        <property type="gene ID" value="Nwi_1123"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1123"/>
    <dbReference type="eggNOG" id="COG1580">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099018_2_1_5"/>
    <dbReference type="OMA" id="DLLIMEF"/>
    <dbReference type="OrthoDB" id="1873899at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005503">
        <property type="entry name" value="FliL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35091">
        <property type="entry name" value="PTHR35091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03748">
        <property type="entry name" value="FliL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="9" id="KW-0969">Cilium</keyword>
    <keyword evidence="8" id="KW-0283">Flagellar rotation</keyword>
    <keyword evidence="9" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA04385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="162" mass="17996" checksum="02CBD948EB268376" modified="2005-10-11" version="1">MAENDQTGKAADGDAPPARKRFKLIAAIGAALFLVTGGSIWFFMFHHSVGEQHAEAQPPKPPSFVDVPDMLVNLVGAPGERVQYLKLKLVLEVKEEPQVEKIRPALPRVTDLFQTYMRELRPSDLNGSAGLFRLKEELTRRVNAVLSPDHVNAVLFKEVVIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STM3</accession>
    <name>Q3STM3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative flagellar protein FlaF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1106</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314399.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1106"/>
    <dbReference type="EnsemblBacteria" id="ABA04368">
        <property type="protein sequence ID" value="ABA04368"/>
        <property type="gene ID" value="Nwi_1106"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1106"/>
    <dbReference type="eggNOG" id="COG5442">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141460_2_0_5"/>
    <dbReference type="OMA" id="MFNRKLW"/>
    <dbReference type="OrthoDB" id="2020092at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044781">
        <property type="term" value="P:bacterial-type flagellum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010845">
        <property type="entry name" value="FlaF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07309">
        <property type="entry name" value="FlaF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0282">Flagellum</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="121" mass="13339" checksum="04E42972D21372B6" modified="2005-10-11" version="1">MSNAAQAYARTAQTSSSPREIEAQALLKAARQLREVQDKWEGPDNDMHKALLFNRRLWSIFMSAADSDSNPQPLEVRQNIANIGVFVMKQTVDMQLNPDPAKLTALIDINCNLAAGLSGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="126" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STS4</accession>
    <name>Q3STS4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">D-alanine--D-alanine ligase</fullName>
            <ecNumber evidence="6">6.3.2.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_1055</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the D-alanine--D-alanine ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.4" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314351.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1055"/>
    <dbReference type="EnsemblBacteria" id="ABA04317">
        <property type="protein sequence ID" value="ABA04317"/>
        <property type="gene ID" value="Nwi_1055"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1055"/>
    <dbReference type="eggNOG" id="COG1181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039268_2_0_5"/>
    <dbReference type="OMA" id="NGELQVM"/>
    <dbReference type="OrthoDB" id="764798at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008716">
        <property type="term" value="F:D-alanine-D-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011095">
        <property type="entry name" value="Dala_Dala_lig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011127">
        <property type="entry name" value="Dala_Dala_lig_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07478">
        <property type="entry name" value="Dala_Dala_lig_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01820">
        <property type="entry name" value="Dala_Dala_lig_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="2 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="5">
        <location>
            <begin position="127"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA04317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="330" mass="35580" checksum="063283F61FC84899" modified="2005-10-11" version="1">MRITILFGGSNKERLVSVASAQALHHALPEADLWFWNLRDEIVDVRSETLRDHARPFEDEFVPGGSGAPLERALDRARAEDRVLVLGLHGGRAENGELQAMCELRRIPFTGSGSAASHLAFDKVAAKRFVASAGVPTPAHVLLEDIDHALAEHGRLVAKPACDGSSYGLIFVNAPQDIVTVREAAKTEDYVIEPFVSGVEATCGVLEHSDGSVVALPPVEIVPAEGAFDYAAKYLAKTTQEICPGRFSPEIAAQIMDHSLRAHRALSCRGYSRTDFIVTGNGPIYLETNTLPGLTAASLYPKALHAQGVGFADFLRDQIVLAEKAVRPNL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STS5</accession>
    <name>MURB_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UDP-N-acetylenolpyruvoylglucosamine reductase</fullName>
            <ecNumber evidence="1">1.3.1.98</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">UDP-N-acetylmuramate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">murB</name>
        <name type="ordered locus">Nwi_1054</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cell wall formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NADP(+) + UDP-N-acetyl-alpha-D-muramate = H(+) + NADPH + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine</text>
            <dbReference type="Rhea" id="RHEA:12248"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:68483"/>
            <dbReference type="ChEBI" id="CHEBI:70757"/>
            <dbReference type="EC" id="1.3.1.98"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MurB family.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.98" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314350.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3STS5"/>
    <dbReference type="SMR" id="Q3STS5"/>
    <dbReference type="STRING" id="323098.Nwi_1054"/>
    <dbReference type="EnsemblBacteria" id="ABA04316">
        <property type="protein sequence ID" value="ABA04316"/>
        <property type="gene ID" value="Nwi_1054"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1054"/>
    <dbReference type="eggNOG" id="COG0812">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035304_1_0_5"/>
    <dbReference type="OMA" id="KMNAGMK"/>
    <dbReference type="OrthoDB" id="841869at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008762">
        <property type="term" value="F:UDP-N-acetylmuramate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.78.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00037">
        <property type="entry name" value="MurB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003170">
        <property type="entry name" value="MurB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011601">
        <property type="entry name" value="MurB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036635">
        <property type="entry name" value="MurB_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21071">
        <property type="entry name" value="PTHR21071"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02873">
        <property type="entry name" value="MurB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56194">
        <property type="entry name" value="SSF56194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00179">
        <property type="entry name" value="murB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0133">Cell shape</keyword>
    <keyword id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000224697" description="UDP-N-acetylenolpyruvoylglucosamine reductase">
        <location>
            <begin position="1"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="1">
        <location>
            <begin position="35"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="228"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00037"/>
        </source>
    </evidence>
    <sequence length="305" mass="32966" checksum="02882F25DCD1D563" modified="2005-10-11" version="1">MTFPDIVPELKSRMPDLRGRLLANESLAPLTWFRVGGPAQALFTPADEDDLAYFLSHLPEEIPVCCIGVGSNLIVRDRGLPGVVIRLPPRGFGEITIDGDAVHAGAAALDKRVAEAAAAASISGLEFYFGIPGTIGGALRMNAGANGSETRDVLVEARALSRRGERMTFDNFAMAFDYRSSGIDPSVIFTGAMFRGRIAEPQAIRARMNEVQAHRETVQPIREKTGGSTFKNPPGQSAWKLIDAAGMRGHRVGGAQVSDMHCNFLINTGEATARDIETLGESVRERVKRHSGVDLQWEIKRIGLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STU4</accession>
    <name>Q3STU4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Universal stress protein, UspA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314331.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1035"/>
    <dbReference type="EnsemblBacteria" id="ABA04297">
        <property type="protein sequence ID" value="ABA04297"/>
        <property type="gene ID" value="Nwi_1035"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1035"/>
    <dbReference type="eggNOG" id="COG0589">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_5_3_5"/>
    <dbReference type="OMA" id="CRESRCA"/>
    <dbReference type="OrthoDB" id="2040681at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Usp" evidence="1">
        <location>
            <begin position="150"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="274" mass="29826" checksum="444B216C7229A971" modified="2005-10-11" version="1">MSYATLMVWVNVDHVSKKFVGVAASLADKFDARLLGLSAVAIFPPFAAPGIVTVANAAEFDIENMKESLIDAGSIVQAAAGSARQVEWRSAIAFPTETLIGEARCADLIVVEGDKLSGDKYRNLDVGSAILGAGRPFLVVPAVVESLAAEHVMIGWKDTREARRAVQDALPFLHEAKRVTVMEICRKEEMETARNRVDDVVRYLAQHRIKAESRVEIQTSRGSAAEQLIGWARGDGVDLLVTGAYGHSRLNEWMFGGMTRDLLNTSPICCLMSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STU6</accession>
    <name>Q3STU6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1033</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1033"/>
    <dbReference type="EnsemblBacteria" id="ABA04295">
        <property type="protein sequence ID" value="ABA04295"/>
        <property type="gene ID" value="Nwi_1033"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1033"/>
    <dbReference type="HOGENOM" id="CLU_2356885_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="96" mass="11023" checksum="23394B408A5C231A" modified="2005-10-11" version="1">MHALTADDSLRGFLPRLPKSMRLTAGDHLIMMACRRHAHLFDHEIRKEGLDDFSAGWASSFRRTECDELIPAFKALPKLLHLMRIETLIPLKIDQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STW7</accession>
    <name>Q3STW7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">PAS/PAC sensor signal transduction histidine kinase</fullName>
            <ecNumber evidence="5">2.7.13.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_1012</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.13.3" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1012"/>
    <dbReference type="EnsemblBacteria" id="ABA04274">
        <property type="protein sequence ID" value="ABA04274"/>
        <property type="gene ID" value="Nwi_1012"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1012"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_23_0_5"/>
    <dbReference type="OMA" id="NAHKTDF"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="4">
        <location>
            <begin position="637"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="3">
        <location>
            <begin position="780"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="221"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="395"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="490"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="578"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="937"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="753"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="411"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="493"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1005" mass="107523" checksum="B0ABF0701184D354" modified="2005-10-11" version="1">MNSAETSADFQLQGMTDPRLAVHAASAMPAWLWSLDGARILWANPVGVSIVGATDASALASRTFNLDDPRRRQVAQLASRLPSSGAVRLERMRGYAGLGRLLTCACSRLDFPGVGSAILVRAAEPAGRGITLVERLQQLVAGVDAPVAAFSHDGRFVAASIKGRSLFGIGSLSEGGLDSLRDQALREGRAETSIGLWRLVLQRVGSGGDIGLVAFLKKNVPDRSPDQAQPLAEPEPESPLQNAETVLPAQAVRSPPEPGNRPSSAPPARRRHPLRFMWRMDAEGRFSIGSDEFTRTIGMRTATGFGRLWSEISDVFGLDPEGRLARAVQTRNTWSGVTLLWPVDGSDARLRVELSGLPIYDRTRNFVGYRGFGVCRDREGLARLAASRRSEFLFSETSHPAPVDSANSADDKPSEPLQQPTEPDTTVETPKNVLPFRPPVDLNPPPLTPVENHAFNELARQLSARLEGEPEAARPEDNAEPPVAAGASAITREQADEPSSPEQPAPRRTTVARDSQILDLIPSGVMIHRAGRALYANAAFLAQVGYQSLETLQEAGGLDALLVEARMVEADPVEAALPDAVGTPPTGSPVTVSVTPPSGAPQQPLAARLFAVSWDGEPAHALICGGASAPSRVGHATAEELATIVDGAAEGLLMFDDGGNITACNHSAETLFGLPGDTLTQRNLVDLFAPESQDAVRDYLKSLTDDDANQLDHGREVVGRMGEDGLIPLSMTMGRTQAAGPNFFAAFRDLSQSKKTETELSQARRQAERAANAKADVLARISHEIRTPLNSIIGFAEVMIDEKFGSLGNERYVEYMKDIRASGERIISFIDDLLDLSRIETGKLDLAFVNQNLNDIVEQCVAVMQPQANRERIIIRTSLAHTLPAIVADAQALRQITLNLIGNSIHLANAGGQVIVSTAVNDFGDVALRVRDSGHSLSEDELAAATEPFRTPPPSDQASKSAGVSLSLTKALVEANRAQFHIKTGPHSGTLIEVVFAQASAPSQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STX5</accession>
    <name>Q3STX5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1004</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1004"/>
    <dbReference type="EnsemblBacteria" id="ABA04266">
        <property type="protein sequence ID" value="ABA04266"/>
        <property type="gene ID" value="Nwi_1004"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1004"/>
    <dbReference type="eggNOG" id="COG5570">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_175516_2_2_5"/>
    <dbReference type="OMA" id="MTIQAHI"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007420">
        <property type="entry name" value="DUF465"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038444">
        <property type="entry name" value="DUF465_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04325">
        <property type="entry name" value="DUF465"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="7"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="61" mass="7156" checksum="994DDC37F3E0C74B" modified="2005-10-11" version="1">MAIQAHLAELERKHRVLESELHDALRHLSSDDLRIAELKRRKLMLKDEIERLKFSTAGTVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU10</accession>
    <name>Q3SU10_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0969</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04231">
        <property type="protein sequence ID" value="ABA04231"/>
        <property type="gene ID" value="Nwi_0969"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0969"/>
    <dbReference type="HOGENOM" id="CLU_1174438_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="236" mass="26727" checksum="5690C805391A70FA" modified="2005-10-11" version="1">MQLEHDPDTKGRASAKWKPVSEKDHARSKIDVESDFNAMNPTLLPVMVEKFARDYRCRQQGSENDELIIRGPSAPAESEAWLYDFDLTRFLHCEPVSTSLENGLQLAVCLMNAELSQSSELKFECLRVLESLDKSSTWRARTDDEDALFFAIVFCERLVPRDDAFPSREPVSIPGSSPRTCFARKRYDVRDECKLALMQCWRGCFGDAVFRSPSMTAGWSTSGLLEDCPVNAKRKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU19</accession>
    <name>Q3SU19_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314263.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0960"/>
    <dbReference type="EnsemblBacteria" id="ABA04222">
        <property type="protein sequence ID" value="ABA04222"/>
        <property type="gene ID" value="Nwi_0960"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0960"/>
    <dbReference type="eggNOG" id="ENOG502Z8S3">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_946052_0_0_5"/>
    <dbReference type="OMA" id="FNSQTIT"/>
    <dbReference type="OrthoDB" id="1965552at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="294" mass="31105" checksum="5B39598CE7E0E387" modified="2005-10-11" version="1">MSRDNRRHDHKDDHNNDPTQNQGQAQANLQAQLQGQANLQGQGQGQGQGQGQGQDQTQIAAQSLYSSSDNTNLNGNGNLNGNWNSNDNKIDNDLDNKIENAVDNKLDNNVDNKLDNSIDNKIENTVENTVETKVDVDVKVDLDLDGSSFANPVIDLSGIHGIEDSLIMPDVVNQTLNGGGNQFNIDQVNSLVDNDHLSNPSVNFNGGGGSGYDWCDPCDPSSGGGFNMEAHVNGATATINGAMGSLDHGSVGVSADSVVDQSAFNQTITLGANIQFNSQTITAGLDSIDDHSMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU57</accession>
    <name>Q3SU57_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">GreA/GreB family elongation factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0922</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314225.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0922"/>
    <dbReference type="EnsemblBacteria" id="ABA04184">
        <property type="protein sequence ID" value="ABA04184"/>
        <property type="gene ID" value="Nwi_0922"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0922"/>
    <dbReference type="eggNOG" id="COG0782">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120358_0_1_5"/>
    <dbReference type="OMA" id="VHCREES"/>
    <dbReference type="OrthoDB" id="1536415at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070063">
        <property type="term" value="F:RNA polymerase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032784">
        <property type="term" value="P:regulation of DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036953">
        <property type="entry name" value="GreA/GreB_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029462">
        <property type="entry name" value="Rnk_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001437">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023459">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30437">
        <property type="entry name" value="PTHR30437"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01272">
        <property type="entry name" value="GreA_GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14760">
        <property type="entry name" value="Rnk_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rnk_N" evidence="2">
        <location>
            <begin position="11"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="GreA_GreB" evidence="1">
        <location>
            <begin position="70"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="151" mass="16779" checksum="E13AB2B96D97AF42" modified="2005-10-11" version="1">MLDNLKKTHLPAIAISAHDIDRLRHLAEAAIEKYPATAEFLAREIDRAEIIPADGSLLDDTRDQRWKVNLVTMQSEVTFIDDVSGQKRRVTLVYPEDADVDAGKISILSPIGAALIGLSAGQSIEFQTPSGGWRSLTVIAVWSYWPRPERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU91</accession>
    <name>Q3SU91_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0886</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314191.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0886"/>
    <dbReference type="EnsemblBacteria" id="ABA04150">
        <property type="protein sequence ID" value="ABA04150"/>
        <property type="gene ID" value="Nwi_0886"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0886"/>
    <dbReference type="eggNOG" id="ENOG50316NY">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032638_0_0_5"/>
    <dbReference type="OrthoDB" id="1300128at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010567">
        <property type="entry name" value="Clostridium_P47"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06597">
        <property type="entry name" value="Clostridium_P47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="403"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="432"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="537" mass="56572" checksum="CEC79E96BBBF7E59" modified="2005-10-11" version="1">MAAAAEKIVVHPSKYAIRIHDPDPMPEPSPPASDVPIADTLGWDTVFAINLPHVNEALRQTGSAPGDFSQTFSSAYAIAGTFGTWQVTMGGDGQDIYLLVPVADGTLDFNGATYDIAGGLATVMINFDYVPGVPKAAPVPVDAKGVPHKLKTKTTTSDPANEPIAAVVQFVFPEGSKASGNTPINRALVQGALTQWFIDNLERFQHVFSTVNLAEKADKASFQWLKPSLTGYAYADGTKPENSLLGVLTTTQRRSIADLSHQIAPSAIPSGAQSGFLISAELFLKQSVLPGLPHAFKKASASDFQLINHGSEITLARGVTVDVNDIDYGGTTYHPKMSSFNLVINDTEIVTNMVVDVDVSPGISVTIMMTNYQTLTLVTKTDGTQTLGYKATRPPRHTYVKHVAAWVKITTAILSVLVAVIGAVVAGPGAALADAIIIGIIVAIIIGIIVGVELIIEDVIAKGVAEAMPSINPMIYAATDPVVWPTAASSFTLTSAGLNGALQFGGTLNFAAPQETPLTAAPPLKVRRSKAAKPSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUB7</accession>
    <name>Q3SUB7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Conjugation TrbI-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0860</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TrbI/VirB10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314169.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0860"/>
    <dbReference type="EnsemblBacteria" id="ABA04124">
        <property type="protein sequence ID" value="ABA04124"/>
        <property type="gene ID" value="Nwi_0860"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0860"/>
    <dbReference type="eggNOG" id="COG2948">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042657_0_0_5"/>
    <dbReference type="OMA" id="HYCERYC"/>
    <dbReference type="OrthoDB" id="1674599at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16429">
        <property type="entry name" value="VirB10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005498">
        <property type="entry name" value="T4SS_VirB10/TraB/TrbI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042217">
        <property type="entry name" value="T4SS_VirB10/TrbI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03743">
        <property type="entry name" value="TrbI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="402" mass="43252" checksum="D3D1398F77D96A0D" modified="2005-10-11" version="1">MTDAPNPSPGDIRAELRLRPEQPRVTRLSRKVLIMLGGVSAAAIMAALFWALDANRRASQSPNELYNTENRTPADGLAGLPKDYTGIPKLGPALPGDLGRPILHAQDEGKPVVAPDIRTPRADPAEQRRLAEIEAARVAKVFTDSRGVQATVASAAAVGRIDPTTGLTLPTETPPLDPGAAQNMQDRKLSFVNAAVDRRTVTPDRVQEKASPYVVQAGTVIPAALITGIKSDLPGTITAQVTEQVYDTPTGKQLLIPQGARLIGQYDSQVAFGQSRVLLVWNRIIMPNGTSIVLERQQGADAQGYSGLEDDVDYHWGQLFKAAILSTLLGVGTEIGASNNENEIARAIRQSSQDTDSDVGRQLIRRQLNIQPTLTIRPGFPVRVIVNRDLILQPYGAAKEPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUF3</accession>
    <name>Q3SUF3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0823</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314136.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0823"/>
    <dbReference type="EnsemblBacteria" id="ABA04088">
        <property type="protein sequence ID" value="ABA04088"/>
        <property type="gene ID" value="Nwi_0823"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0823"/>
    <dbReference type="HOGENOM" id="CLU_2437839_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="90" mass="10112" checksum="28F49896C5E3BFA9" modified="2005-10-11" version="1">MIAPLMIAERLEARALAANAVRSAFYERFGEHPSAEDRLWQIDPIARLDFVGDVELKLGVAFRDEDVEFLDTPSDLIERGAEILIRGGVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUM0</accession>
    <name>Q3SUM0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cobalamin synthesis protein, CobW</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0756</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIMIBI class G3E GTPase family. CobW subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314075.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0756"/>
    <dbReference type="EnsemblBacteria" id="ABA04021">
        <property type="protein sequence ID" value="ABA04021"/>
        <property type="gene ID" value="Nwi_0756"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0756"/>
    <dbReference type="eggNOG" id="COG0523">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017452_0_1_5"/>
    <dbReference type="OMA" id="HDCHDHH"/>
    <dbReference type="OrthoDB" id="1267701at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011629">
        <property type="entry name" value="Cbl_biosynth_CobW-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036627">
        <property type="entry name" value="CobW-likC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07683">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00833">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobW C-terminal" evidence="3">
        <location>
            <begin position="258"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="218"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="353" mass="40269" checksum="23846DD2C450E59B" modified="2005-10-11" version="1">MTDKIPVTVLTGYLGAGKTTLLNRILSEEHGRKYAVIVNEFGEIGIDNDLVVGADEEVFEMNNGCICCTVRGDLMRILADLMKRNGKFDAIIVETTGLADPAPVAQTFFVDENVGKKTKLDAVVTVTDAKWLNDRLKDAPEAKNQIAFADVILINKTDLVSPEELSEIEARIRGINPYAKLHRTQRAQIALSEILERNAFDLDRILDIEPAFLERKDHDHDHHHDHGHDHHHDHDHHDHDHKHDDHHGGVKHYHDEEMQSISLKTDKPLDADKFFPWVEDLVQKEGPNILRSKGILSFKDEDRRFVFQGVHMILDGDHQRPWKDDEKRQSRIVFIGRNLPEQLITEGFEGCIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUT3</accession>
    <name>Q3SUT3_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Coenzyme PQQ synthesis protein B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Pyrroloquinoline quinone biosynthesis protein B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">pqqB</name>
        <name evidence="8" type="ordered locus">Nwi_0692</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">May be involved in the transport of PQQ or its precursor to the periplasm.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Cofactor biosynthesis; pyrroloquinoline quinone biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the PqqB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314014.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0692"/>
    <dbReference type="EnsemblBacteria" id="ABA03958">
        <property type="protein sequence ID" value="ABA03958"/>
        <property type="gene ID" value="Nwi_0692"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0692"/>
    <dbReference type="eggNOG" id="COG1235">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061120_0_0_5"/>
    <dbReference type="OMA" id="FYIPGCA"/>
    <dbReference type="OrthoDB" id="1712770at2"/>
    <dbReference type="UniPathway" id="UPA00539"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018189">
        <property type="term" value="P:pyrroloquinoline quinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00653">
        <property type="entry name" value="PQQ_syn_PqqB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011842">
        <property type="entry name" value="PQQ_synth_PqqB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02108">
        <property type="entry name" value="PQQ_syn_pqqB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0884">PQQ biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Lactamase_B" evidence="7">
        <location>
            <begin position="48"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA03958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="299" mass="32184" checksum="7D0A35161F2C5EAC" modified="2005-10-11" version="1">MRFVVLGAAAGGGLPQWNCGCRNCAMARSSGLRPQTQSSLAVSLGGLNWAILNASPDIRQQINDNTQLHPRGPRDTPIMSVVLTNGDLDHIVGLLTLRERQPFQIFLTGAIAGILDSNPVFRVLDPNTVQRNLVVPNKSFPLLPGLDASLFAVPGKVALFMEDEDATIGAESEHVTGVALSAGGLRAFYIPGCAHLTESLATRIRGADIVFFDGTLFTDDEMIRTRTGTKTGRRMGHMPISGEGGSLKVLARLDIGRIVYIHINNTNPIWRDGPERDLVLRHGFEVGCDGAEISLAPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUV2</accession>
    <name>Q3SUV2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Binding-protein-dependent transport systems inner membrane component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0673</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0673"/>
    <dbReference type="EnsemblBacteria" id="ABA03939">
        <property type="protein sequence ID" value="ABA03939"/>
        <property type="gene ID" value="Nwi_0673"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0673"/>
    <dbReference type="eggNOG" id="COG0600">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046113_1_1_5"/>
    <dbReference type="OMA" id="SAGYSFF"/>
    <dbReference type="OrthoDB" id="1531811at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="186"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="233"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="80"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="275" mass="30087" checksum="F2B9F66762374C31" modified="2005-10-11" version="1">MLTDAPAEPRRIVSLPNGFRSAALSVLIAAASLSAGLLFWYWATLTSLDFYVRFDNVPTPIQVANAFWHELGDKRFYFHILYSLRRIAIAYALASALGIGLGLAVGRSRLARAVILPYVEIIRPIPAVAWIPLAILMWPTEESSIIYITALGAMFPILLNTIHGVEQTPEILVRAAQSLGASRLAIFQHVVLPSALPHIVTGLAIGMGVSWFSLLAGEIISGQYGIGYFTWNAYSLILYPEIVVGMIVIGLLGTASTHAVRLLTRPLLAWQRNPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV13</accession>
    <name>Q3SV13_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="13">ABC transporter, transmembrane region</fullName>
            <ecNumber evidence="13">3.6.3.27</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Nwi_0611</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in beta-(1--&gt;2)glucan export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.27" evidence="13"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313938.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0611"/>
    <dbReference type="EnsemblBacteria" id="ABA03878">
        <property type="protein sequence ID" value="ABA03878"/>
        <property type="gene ID" value="Nwi_0611"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0611"/>
    <dbReference type="eggNOG" id="COG2274">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_3_5"/>
    <dbReference type="OMA" id="SHFINIM"/>
    <dbReference type="OrthoDB" id="643917at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="3 10 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="143"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="265"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="12">
        <location>
            <begin position="37"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="351"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="385"/>
            <end position="392"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA03878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="573" mass="60805" checksum="9856D043789265D0" modified="2005-10-11" version="1">MNAWDALVEGRLGSGPRAFWELYREACRAEAGRFAPAIAASLALGLLAVLPPILIAQLIDRAEAGVGAMGLLALGVLAAFAALALLDAGLTWLRHRFTIAAQVRLRARIAPALLITGLRLPLHKLRDNSRATMMRSFDDLDETLGFIAGTMPEFVTAAILAASYAVLLLVVHPLLAVVSLGATALAFVTAAVLARRSRNAFASWILDRDRAFTVIVEAFASLLTIKSMNAHRAIADRFADKLKAENASLAAMRQRIADTEAASRGWLTFVPGIVVVVGVWMVSRGELSIGELVLFLTVSASLSGPVGSMCTHWEAAQRTMAAIARIAELRAAELEDLDADSGRDWSPDAGLSLAGVGHRYAADAAPVLRAVDLALDSGSHIAVTGRSGGGKTTLAHIVARFMQPSEGAVLAGAADAAGIGLTPYRRRVLMLPHETEIFSASIADNVTLWDRSFCLPEVMEALRIAGLGEVVDGLPRRHDTPLSADGEPLSAGQRQRLGIARALIRRPDVLILDEATSSLDPQTEAEVIANVRAVMQGRTLIVITHREELAARFGRRLAVKGGGAAYLPGLEPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV37</accession>
    <name>Q3SV37_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidase C51 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0587</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313915.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0587"/>
    <dbReference type="EnsemblBacteria" id="ABA03854">
        <property type="protein sequence ID" value="ABA03854"/>
        <property type="gene ID" value="Nwi_0587"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0587"/>
    <dbReference type="eggNOG" id="COG3409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083609_1_0_5"/>
    <dbReference type="OMA" id="SLWCARF"/>
    <dbReference type="OrthoDB" id="682655at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007921">
        <property type="entry name" value="CHAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013423">
        <property type="entry name" value="CHP02594"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05257">
        <property type="entry name" value="CHAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02594">
        <property type="entry name" value="TIGR02594"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase C51" evidence="1">
        <location>
            <begin position="115"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="208" mass="23221" checksum="F0D3D9D137E78EA1" modified="2005-10-11" version="1">MFQLVALRWMPRILALAVCFVAVVAFAMPASARPYHRAGHHAHVHHYKHHHHIRRHYGYRHHRYRHARRSHARGWRNASAALTPKSGFSGGGLVSNIVAEARRYIGGNPTGRRRLWCARFMNMVLKRAGHQGTGSDMARSFAHYGRRVSGPQVGAIAVMTRRGGGGHVGVVSGVDRRGNPIVVSGNHGRRVAESVYPRSRVYAYVVPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV54</accession>
    <name>Q3SV54_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">FAD-dependent pyridine nucleotide-disulfide oxidoreductase</fullName>
            <ecNumber evidence="5">1.18.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0570</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.18.1.3" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313898.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0570"/>
    <dbReference type="EnsemblBacteria" id="ABA03837">
        <property type="protein sequence ID" value="ABA03837"/>
        <property type="gene ID" value="Nwi_0570"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0570"/>
    <dbReference type="eggNOG" id="COG0446">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2146">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003291_4_2_5"/>
    <dbReference type="OMA" id="PWHKAAF"/>
    <dbReference type="OrthoDB" id="1149616at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008860">
        <property type="term" value="F:ferredoxin-NAD+ reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028202">
        <property type="entry name" value="Reductase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14759">
        <property type="entry name" value="Reductase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="SSF50022"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="SSF55424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="4">
        <location>
            <begin position="14"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="506" mass="55202" checksum="51220BC1798E4B7D" modified="2005-10-11" version="1">MAQEQAQPSGPDLTQGVDPGDFKDGKLVGHVGDQEILLVREGEGIFAIDAHCSHYHGPLADGIVVDGTVRCPWHHACFDLRTGEATCAPAFNALDVWQVEQRDGRIFVREKRARPAVRIAKPDHPGKIVIVGGGAAGFAAAEMLRRQDYAGSIVMLSSDDAPPVDRPNLSKDYLAGSAPEDWLPLRPDDFYQQAGIDLRLRTTVTAIDPSARQLSINGDVIGYDRLLLATGAEPVRLPIPGANLSHVHTLRSLADCRAIIDGAKSANRAVVIGASFIGLEVAASLRARGIEVHVVAPESRPMERILGAEMGDFVRSLHEEHGVIFHLENTVTAIGEKKVTISSGETLEADFVVFGVGVKPRLELAEKAGLKIDRGVLVNQYLETSAPGIFAAGDIARWPDPRFGENIRVEHWVVAQRQGQVAARNMLGHREKYDAVPFFWSQHYDVPINYVGHALKWDQIEVEGSVADRDCLLRYKHLGRVLAVSSIYRDVDHLKAEVAMERAVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV87</accession>
    <name>Q3SV87_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Sel1-like repeat protein</fullName>
            <ecNumber evidence="1">3.5.2.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0537</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.2.6" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313865.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0537"/>
    <dbReference type="EnsemblBacteria" id="ABA03804">
        <property type="protein sequence ID" value="ABA03804"/>
        <property type="gene ID" value="Nwi_0537"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0537"/>
    <dbReference type="eggNOG" id="COG0790">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_36_14_5"/>
    <dbReference type="OMA" id="CEEWYER"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008800">
        <property type="term" value="F:beta-lactamase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="680" mass="73395" checksum="7E0472698A1EE4C0" modified="2005-10-11" version="1">MLSSRSLLQWVRRPVLGPSRGGLFEHGMGAYERGEYLEAVRCWKLALEAGEAEAAYRIGRLYVKGEGVVRSVPDAAIWYERAARLGHVDAQFQLGLIFLQGEKPLLGPHRHETWRQSSAIRLGDKASNIHQLVFPHGTSVERNVDAAFHWISAAADKGKAEAQTVLGNMYSEGLGCEKNLQIALAWYGVAAEQNCAAAEFALGDIHFQGKGVPVDFEQAAVWYRKAAEQDHVRAQVALAFMNLKGTGMPENPAEAARLFQGAAMHDDIIALYNIGLLRLKGHGVAKDIDKAETALRKAARKDYFPAIQALAEFYSHGGGFAPDLREAAVWYEKAAERDDVQAQFFMGRFYAMGTGVGPNIRQAAKWFERAARNGHATAAFNIAIFYLNGSGVERDVDRAIEWFERASEGGIRAAQLQLGKLYSAGNGVPRDQKLAREWLGKAANGGDPDAQTAYALFLLRQDGSAEQLEQAKALLVEAAEADHAPAAFQLGVLHMGKFGGETDIAAAVPWFARAAGAGHVDAQYTLALLHLDPGSGMSDAKAAASWMTKAAHAGHAGAQFQLAVLYCTGAGLAQDVAQGVRWYEAAAQQGHRIAQFNLAVMLGKGQGCEVDLGKAVEWFEKAARQDVAEAQIALGDALMSGSGVTKDQDAAVQWYRRAAGHNHEGARHRLNAIGVTTVDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVD5</accession>
    <name>Q3SVD5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Amine oxidase</fullName>
            <ecNumber evidence="3">1.4.3.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0489</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.4.3.4" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0489"/>
    <dbReference type="EnsemblBacteria" id="ABA03756">
        <property type="protein sequence ID" value="ABA03756"/>
        <property type="gene ID" value="Nwi_0489"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0489"/>
    <dbReference type="eggNOG" id="COG1231">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004498_10_3_5"/>
    <dbReference type="OMA" id="LEPGNRW"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097621">
        <property type="term" value="F:monoamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amino_oxidase" evidence="2">
        <location>
            <begin position="57"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="434"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="442"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="456" mass="48986" checksum="2D608CC3AE82B25E" modified="2005-10-11" version="1">MIRSKLNRTILQKWLSGRIFLRRTGIHFAGKCSNQRGDMSQNLPSNVDVAIIGAGAAGLGAADALNDSGLSVIVLEARGRVGGRAHTIFPAPGIVFDLGCGWLHSADRNSFVAIAGRLGFEIDRTPPPWREQSFAAGFTEAEREDFLAALDAFDDRIQKAAKNDADADDDASRYLEPGNHWNPVIDAVSTYVNGCELDGVSVRDTAAYEDTKINWRVRHGYGAMIAAYARPCPVALNTRVWRIDHSATRIRIETSNGVLSATQVIVTVPTSLLANESIRFHPELPAKVNAAADLPLGIDDKVMLALDDPGNTAPKDGNLSGAIMRTKIGTYHLRPFGQPCIEGFFGGSFARELEAAGNGAFAAQAIDEITALLGSSYRRKLKPLAESRWASDPFAQGAYSHALPGRACARAVLAAPIDGRLFFAGEATSPNFFSTAHGARDSGERAAQEAMAARRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVD7</accession>
    <name>Q3SVD7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain dehydrogenase/reductase SDR</fullName>
            <ecNumber evidence="1">1.1.1.100</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0487</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.100" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313815.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0487"/>
    <dbReference type="EnsemblBacteria" id="ABA03754">
        <property type="protein sequence ID" value="ABA03754"/>
        <property type="gene ID" value="Nwi_0487"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0487"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_5"/>
    <dbReference type="OMA" id="ERVEFHK"/>
    <dbReference type="OrthoDB" id="1821409at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="257" mass="26827" checksum="4CE4B59B020CEECF" modified="2005-10-11" version="1">MTDHSIKGKTAIIAGGAKNLGALLARDLAAHGAKAIAIHYNSAAAKKDADQTLEAIKAAGADGVAFQGDLTSASAMEKLFADTIAAIGRPDIAINTVGKVLKKPMADISEAEFDAMSAVNAKTAFFFLKEAGKAVNDNGKICTLVTSLLGAFTPFYAAYAGMKAPVEHFTRAAAKEFGERGISVTAIGPGPMDTPFFYPAEGADAVAYHKTAAALSKFTSTGLTDIQDIVPWIRFLVSDGWWMTGQTILVNGGYTTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVH5</accession>
    <name>Q3SVH5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">C-terminal processing peptidase-3, Serine peptidase, MEROPS family S41A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_0449</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the peptidase S41A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313780.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0449"/>
    <dbReference type="MEROPS" id="S41.004"/>
    <dbReference type="EnsemblBacteria" id="ABA03716">
        <property type="protein sequence ID" value="ABA03716"/>
        <property type="gene ID" value="Nwi_0449"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0449"/>
    <dbReference type="eggNOG" id="COG0793">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017295_3_1_5"/>
    <dbReference type="OMA" id="TFNQVDW"/>
    <dbReference type="OrthoDB" id="1646508at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07560">
        <property type="entry name" value="Peptidase_S41_CPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004447">
        <property type="entry name" value="Peptidase_S41A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005151">
        <property type="entry name" value="Tail-specific_protease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03572">
        <property type="entry name" value="Peptidase_S41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00245">
        <property type="entry name" value="TSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00225">
        <property type="entry name" value="prc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="92"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="378"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="428"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="378"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA03716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="451" mass="48409" checksum="EA61A2B238EC57C6" modified="2005-10-11" version="1">MMRKVSLIVLSAAAGAALTLFVTQPRSVLMGSSARAATSDTYRQLNLFGDVFERVRTDYVEKPDDSKLVESAISGMLTGLDPHSSYMDAKSFRDMQVQTRGEFGGLGIEVTMEDGLVKVVSPIDDTPASKAGILANDIITNLDDEAVQGLTLNQAVDKMRGPIGTKIKLKIIRKGQDNPIDVTLVRDNIRVRSVRSRTESDDIAYIRITTFNEQTTEGLKKSVSDLQSQIGDKLKGYIIDLRNNPGGLLEEAVTVSDAFLDRGEIVSTRGRNAEETQRRSAHPGDLAKGKPVIILVNGGSASASEIVAGALQDHKRATLIGTRSFGKGSVQTIIPLGSGNGALRLTTARYYTPSGRSIQAKGIVPDIEVLQDVPDELRSRTDTKGEASLRGHLRNGNDEKTGSQSYVPPDAKNDKALKMAGDLLRGVKINASSPPSNNKAAIEKPANKAAN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVJ3</accession>
    <name>ATPG_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP synthase gamma chain</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP synthase F1 sector gamma subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">F-ATPase gamma subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpG</name>
        <name type="ordered locus">Nwi_0431</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase gamma chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313762.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVJ3"/>
    <dbReference type="SMR" id="Q3SVJ3"/>
    <dbReference type="STRING" id="323098.Nwi_0431"/>
    <dbReference type="EnsemblBacteria" id="ABA03698">
        <property type="protein sequence ID" value="ABA03698"/>
        <property type="gene ID" value="Nwi_0431"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0431"/>
    <dbReference type="eggNOG" id="COG0224">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050669_0_1_5"/>
    <dbReference type="OMA" id="MQITSAM"/>
    <dbReference type="OrthoDB" id="1701531at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045261">
        <property type="term" value="C:proton-transporting ATP synthase complex, catalytic core F(1)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12151">
        <property type="entry name" value="F1-ATPase_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00815">
        <property type="entry name" value="ATP_synth_gamma_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035968">
        <property type="entry name" value="ATP_synth_F1_ATPase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000131">
        <property type="entry name" value="ATP_synth_F1_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023632">
        <property type="entry name" value="ATP_synth_F1_gsu_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11693">
        <property type="entry name" value="PTHR11693"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00231">
        <property type="entry name" value="ATP-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00126">
        <property type="entry name" value="ATPASEGAMMA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52943">
        <property type="entry name" value="SSF52943"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01146">
        <property type="entry name" value="ATPsyn_F1gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00153">
        <property type="entry name" value="ATPASE_GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0139">CF(1)</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_1000053271" description="ATP synthase gamma chain">
        <location>
            <begin position="1"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00815"/>
        </source>
    </evidence>
    <sequence length="292" mass="32140" checksum="24E97F576DDE00D7" modified="2005-10-11" version="1">MASLKDMRVRIASTKATQKITKAMQMVAASKLRRAQNAAEAARPYAEKMDTVISNIASAAAGSPNASPLLAGTGKDQVHLLLVCTGERGLSGAFNSAIVRLARDRAFALMNQGKEVKFFCVGRKGYEQLRRIFEKQIVETVELRTVRQLGFVNAEDIARKVLARFDAGEFDVCTLFYSRFQSVIAQVPTAQQVIPLAVEDAARSDGPAPSYEYEPEEDEILNGLLPRNLAVQIFRALLENNASFYGAQMTAMDNATRNAGEMIRKQTLIYNRTRQAMITKELIEIISGAEAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVM2</accession>
    <name>Q3SVM2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0402</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313733.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0402"/>
    <dbReference type="EnsemblBacteria" id="ABA03669">
        <property type="protein sequence ID" value="ABA03669"/>
        <property type="gene ID" value="Nwi_0402"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0402"/>
    <dbReference type="eggNOG" id="ENOG503342I">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150610_0_0_5"/>
    <dbReference type="OMA" id="VDVAEDH"/>
    <dbReference type="OrthoDB" id="1712653at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013433">
        <property type="entry name" value="PHA_gran_rgn"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09650">
        <property type="entry name" value="PHA_gran_rgn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="107" mass="11965" checksum="E8F5C61680803241" modified="2005-10-11" version="1">MTRPLVVSIPHRLGKDEALRRMKAGLGSARTQYAQLIQVNEEVWSGDRLVFSVTALKQQVSGILDVSEDHVRLEVTLPWLLSRLAQGIQGVIQKKGTLMLEKKTDES</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVP4</accession>
    <name>Q3SVP4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0380</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0380"/>
    <dbReference type="EnsemblBacteria" id="ABA03647">
        <property type="protein sequence ID" value="ABA03647"/>
        <property type="gene ID" value="Nwi_0380"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0380"/>
    <dbReference type="HOGENOM" id="CLU_2233674_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="105" mass="11753" checksum="499FF35AD20D3542" modified="2005-10-11" version="1">MLDGKKLADDNASSMKREKRGRTGEKARIHNGADAGRQPGFRSCRGRPNDPVNVVRDQRAVSAIPQPSRPWCIRATCPYLRFDAISPLIAVAAFIRVEDVRDRDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVU9</accession>
    <name>Q3SVU9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313657.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0325"/>
    <dbReference type="EnsemblBacteria" id="ABA03592">
        <property type="protein sequence ID" value="ABA03592"/>
        <property type="gene ID" value="Nwi_0325"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0325"/>
    <dbReference type="eggNOG" id="ENOG5032RW3">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133774_1_0_5"/>
    <dbReference type="OMA" id="DAILWIY"/>
    <dbReference type="OrthoDB" id="1685766at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021252">
        <property type="entry name" value="DUF2794"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10984">
        <property type="entry name" value="DUF2794"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="123" mass="14019" checksum="78EA44E14F285508" modified="2005-10-11" version="1">MSSLPDDLDPGGSRVTERDPAPVPIPGRVTFNRLELNRILNLYGRMVADGDWRDYAIDFLRDRAVFSVFRRASEVPIYRIEKDPRLARKQGAYSVISASGLILRRGHELDRVLLVIDRKLSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVY6</accession>
    <name>Q3SVY6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Extracellular solute-binding protein, family 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0288</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial solute-binding protein 5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313621.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0288"/>
    <dbReference type="EnsemblBacteria" id="ABA03555">
        <property type="protein sequence ID" value="ABA03555"/>
        <property type="gene ID" value="Nwi_0288"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0288"/>
    <dbReference type="eggNOG" id="COG4166">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023171_0_0_5"/>
    <dbReference type="OMA" id="VLPKHWW"/>
    <dbReference type="OrthoDB" id="162819at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030288">
        <property type="term" value="C:outer membrane-bounded periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030678">
        <property type="entry name" value="Peptide/Ni-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PTHR30290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002741">
        <property type="entry name" value="MppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0574">Periplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SBP_bac_5" evidence="4">
        <location>
            <begin position="121"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="631" mass="71423" checksum="05C24A6C5F818A82" modified="2005-10-11" version="1">MAFTRRRLLHGGALIAAAPAIKVLTGASSIQAAHAQAAPAGLKWRHGLSLFGEVKYPEGFKHFDYVNPDAPKGGLVRQIAIGTFDNFNPVVSGVKGSIASEVRLVYEALTAPSLDEVSTEYGALAESVAYPDDFSFVVYRLRAEAKWHDGKPVTPDDVIFSLNVLKKHHPFYGAYYRHITKVEKTGERDVTFTFDAPGNRELPQIVGQLMVLPKHWWEGTDDAGRKRDISATTLEPPLGSGPYRIKRFDAARSVTLERVKDYWGRDLNFSIGHNNFDELRYEYFRDGTVALEAFKGDQVDWRTENSAKNWATAYDFPAVKDGRVILEEFPNRNAGVMQAFALNIRRDKFRDPRVRRALNFAFNFEEMNKQLFYGQYKRINSYFEGTELASSGLPEGRELEILETVRAEVPAEVFTTPYSNPVGGNPKAVRNNLREGMKLFKEAGFEVRNHKLVDGKTGAPFELELLSEAPSTERIMLFYKPSLERMGITVSVRTVDSTQYENRMRSWDFDVVGNLWIESLSPGNEQREFWGSQAADMPGSRNVVGIKNPAVDKLIERVIFAKDRADLVAATKALDRVLLWNHYVVPQFTYPFHRTARWDRFGRPEKMPKYGLSAFPTLWWHDAGKAAGKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVY9</accession>
    <name>Q3SVY9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Prephenate dehydratase</fullName>
            <ecNumber evidence="3">4.2.1.51</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_0285</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + prephenate = 3-phenylpyruvate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:21648"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:18005"/>
            <dbReference type="ChEBI" id="CHEBI:29934"/>
            <dbReference type="EC" id="4.2.1.51"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-phenylalanine biosynthesis; phenylpyruvate from prephenate: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.51" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313618.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0285"/>
    <dbReference type="EnsemblBacteria" id="ABA03552">
        <property type="protein sequence ID" value="ABA03552"/>
        <property type="gene ID" value="Nwi_0285"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0285"/>
    <dbReference type="eggNOG" id="COG0077">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035008_4_2_5"/>
    <dbReference type="OMA" id="PLMIYRE"/>
    <dbReference type="OrthoDB" id="1280729at2"/>
    <dbReference type="UniPathway" id="UPA00121">
        <property type="reaction ID" value="UER00345"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004106">
        <property type="term" value="F:chorismate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004664">
        <property type="term" value="F:prephenate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009094">
        <property type="term" value="P:L-phenylalanine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008242">
        <property type="entry name" value="Chor_mutase/pphenate_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001086">
        <property type="entry name" value="Preph_deHydtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00800">
        <property type="entry name" value="PDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001500">
        <property type="entry name" value="Chor_mut_pdt_Ppr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51171">
        <property type="entry name" value="PREPHENATE_DEHYDR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0584">Phenylalanine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prephenate dehydratase" evidence="9">
        <location>
            <begin position="6"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="10">
        <location>
            <begin position="196"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="site" description="Essential for prephenate dehydratase activity" evidence="8">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001500-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA03552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="286" mass="31250" checksum="B20DE32ED98AE7D9" modified="2005-10-11" version="1">MTRTMKIVFQGEPGANSHIAIAEAYPDAEPLPCATFEDALAAITSGEADLGMIPIENSVAGRVADIHHLLPQSGLFIVGEYFLPVRHQLMAPRGARLADIKTVESHVHALGQCRRIIRKLGIRPIVSGDTAGAARLVAERGDTSCAALASRLASQIHHLDILAENVEDEDHNTTRFVVLAREADWARQGSGPLVTSFIFQVRNLPAALYKAMGGFATNSVNMTKLESYMVDGNFLATQFYADVEGHPNDRGLAFALEELKFFSKELRIVGVYPAHPFRATFRERAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW69</accession>
    <name>Q3SW69_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0204</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313540.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0204"/>
    <dbReference type="EnsemblBacteria" id="ABA03472">
        <property type="protein sequence ID" value="ABA03472"/>
        <property type="gene ID" value="Nwi_0204"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0204"/>
    <dbReference type="eggNOG" id="COG5488">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096000_1_0_5"/>
    <dbReference type="OMA" id="WVLNPLW"/>
    <dbReference type="OrthoDB" id="1791802at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019253">
        <property type="entry name" value="DUF2244_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016990">
        <property type="entry name" value="UCP032162_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10003">
        <property type="entry name" value="DUF2244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032162">
        <property type="entry name" value="UCP032162_imp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="174" mass="19487" checksum="D4FC2DF1F582AB04" modified="2005-10-11" version="1">MTVDNNLAGPGPAEQTLFSALLTPHRSLSHASFMVLMAVLALASFATGMVFLLMGAWPVFGFLGLDVLAIYWAFRINFQRALATEEISITHSELRVRRTSHRGHIVEWVSNPLWVHVDQRVHEEYGIERLYLVSRGRRISIANFLGPDEKLSFVKALSSALQLAKRGPIYNPVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWA6</accession>
    <name>Q3SWA6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">TPR repeat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_0167</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the kinesin light chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313504.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0167"/>
    <dbReference type="EnsemblBacteria" id="ABA03435">
        <property type="protein sequence ID" value="ABA03435"/>
        <property type="gene ID" value="Nwi_0167"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0167"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_125_15_5"/>
    <dbReference type="OMA" id="RALVIWK"/>
    <dbReference type="OrthoDB" id="308403at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002151">
        <property type="entry name" value="Kinesin_light"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00381">
        <property type="entry name" value="KINESINLIGHT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="51"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="93"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="135"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA03435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="190" mass="21007" checksum="64BA4FA9746E0949" modified="2005-10-11" version="1">MAVSDIMLAARLNDQAELFREKGRYDEAEPLYKQALAIFAKARGFDHPSVALALTDLAELYTLQNRYDEAEPLYQRALVIWKKAVGPHHPDIVQSLNSLANLYSVQGRYAEAEPLYKRAIAIFEKSLGANHPSVASALDNLASIYKAEARYAEAEPLYKTALAIREKSLGPDHPDVARSRDNLAGLPGAQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWE4</accession>
    <name>HSLU_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent protease ATPase subunit HslU</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Unfoldase HslU</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hslU</name>
        <name type="ordered locus">Nwi_0129</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N-terminal part of its protein substrates and unfolds these before they are guided to HslV for hydrolysis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">A double ring-shaped homohexamer of HslV is capped on each side by a ring-shaped HslU homohexamer. The assembly of the HslU/HslV complex is dependent on binding of ATP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ClpX chaperone family. HslU subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313466.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWE4"/>
    <dbReference type="SMR" id="Q3SWE4"/>
    <dbReference type="STRING" id="323098.Nwi_0129"/>
    <dbReference type="EnsemblBacteria" id="ABA03397">
        <property type="protein sequence ID" value="ABA03397"/>
        <property type="gene ID" value="Nwi_0129"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0129"/>
    <dbReference type="eggNOG" id="COG1220">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033123_0_0_5"/>
    <dbReference type="OMA" id="KYGMIKT"/>
    <dbReference type="OrthoDB" id="718259at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009376">
        <property type="term" value="C:HslUV protease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036402">
        <property type="term" value="F:proteasome-activating activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043335">
        <property type="term" value="P:protein unfolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00249">
        <property type="entry name" value="HslU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004491">
        <property type="entry name" value="HslU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48102:SF3">
        <property type="entry name" value="PTHR48102:SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00390">
        <property type="entry name" value="hslU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0346">Stress response</keyword>
    <feature type="chain" id="PRO_1000012765" description="ATP-dependent protease ATPase subunit HslU">
        <location>
            <begin position="1"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="60"/>
            <end position="65"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="383"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00249"/>
        </source>
    </evidence>
    <sequence length="433" mass="47559" checksum="3F25089AC031A18C" modified="2005-10-11" version="1">MTDISPREIVSELDRFIVGQADAKRAVSIALRNRWRRQQLTGPLREEVLPKNILMIGPTGVGKTEIARRLAKLANAPFLKIEATKFTEVGYVGRDVEQIVRDIVEVAINQTRERKRKDVQARAQLAAEERVLDALVGANASAATRDSFRKRLRSGELNDKEIEIETQTSGGGPMFEIPGMPGAQVGAISIGDIFGKMGGRTKTRRLTVSDSYEILINEESDKLLDSDQLTQEAIAAVENNGIVFLDEIDKICVRDGRSGGDVSREGVQRDLLPLIEGTTVSTKHGAVKTDHILFIASGAFHIAKPSDLLPELQGRLPIRVELDALTRDDLRRILTEPEASLIKQYVALMDTEGVVLDFTDDAVDALADIAVAVNSTVENIGARRLQTVMERVLDDISFTAPDRNGETVRIDAGYVQKNIGDLAKNADLSRFIL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-05-25" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWE9</accession>
    <name>HIS51_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Imidazole glycerol phosphate synthase subunit HisH 1</fullName>
            <ecNumber evidence="1">4.3.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">IGP synthase glutaminase subunit 1</fullName>
            <ecNumber evidence="1">3.5.1.2</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">IGP synthase subunit HisH 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">ImGP synthase subunit HisH 1</fullName>
            <shortName evidence="1">IGPS subunit HisH 1</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisH1</name>
        <name type="ordered locus">Nwi_0124</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to HisF for the synthesis of IGP and AICAR.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H(+) + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="4.3.2.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-glutamine = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of HisH and HisF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="4.3.2.10" evidence="1"/>
    <dbReference type="EC" id="3.5.1.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313461.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWE9"/>
    <dbReference type="SMR" id="Q3SWE9"/>
    <dbReference type="STRING" id="323098.Nwi_0124"/>
    <dbReference type="EnsemblBacteria" id="ABA03392">
        <property type="protein sequence ID" value="ABA03392"/>
        <property type="gene ID" value="Nwi_0124"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0124"/>
    <dbReference type="eggNOG" id="COG0118">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071837_2_0_5"/>
    <dbReference type="OMA" id="WVYFVHS"/>
    <dbReference type="OrthoDB" id="1726024at2"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000107">
        <property type="term" value="F:imidazoleglycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01748">
        <property type="entry name" value="GATase1_IGP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00278">
        <property type="entry name" value="HisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010139">
        <property type="entry name" value="Imidazole-glycPsynth_HisH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42701">
        <property type="entry name" value="PTHR42701"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000495">
        <property type="entry name" value="Amidotransf_hisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01855">
        <property type="entry name" value="IMP_synth_hisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000231737" description="Imidazole glycerol phosphate synthase subunit HisH 1">
        <location>
            <begin position="1"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1" evidence="1">
        <location>
            <begin position="2"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00278"/>
        </source>
    </evidence>
    <sequence length="216" mass="23431" checksum="24B2C4103CF36272" modified="2005-10-11" version="1">MTAAIIDYGSGNLHSAAKALECAARGMEGLQRIVVTRDPDVVFRADRVVLPGVGAFADCRRGLDALDGMVQALTEVVRDKARPFLGICVGMQLMATRGKEHVTTDGLGWIAGDVERISPRDESLKIPHMGWNTLDPAREHPVLEGLPLGPKGRHAYFVHSYHLNAANETDVLARADYGGPVTAIVGKDTAIGTQFHPEKSQRFGLALIANFLRWKP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWF3</accession>
    <name>COAA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pantothenate kinase</fullName>
            <ecNumber evidence="1">2.7.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Pantothenic acid kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">coaA</name>
        <name type="ordered locus">Nwi_0120</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-pantothenate + ATP = (R)-4'-phosphopantothenate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16373"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29032"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 1/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the prokaryotic pantothenate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.33" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313457.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWF3"/>
    <dbReference type="SMR" id="Q3SWF3"/>
    <dbReference type="STRING" id="323098.Nwi_0120"/>
    <dbReference type="EnsemblBacteria" id="ABA03388">
        <property type="protein sequence ID" value="ABA03388"/>
        <property type="gene ID" value="Nwi_0120"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0120"/>
    <dbReference type="eggNOG" id="COG1072">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053818_1_1_5"/>
    <dbReference type="OMA" id="RKYTQVS"/>
    <dbReference type="OrthoDB" id="1793376at2"/>
    <dbReference type="UniPathway" id="UPA00241">
        <property type="reaction ID" value="UER00352"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004594">
        <property type="term" value="F:pantothenate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02025">
        <property type="entry name" value="PanK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00215">
        <property type="entry name" value="Pantothen_kinase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004566">
        <property type="entry name" value="PanK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285:SF139">
        <property type="entry name" value="PTHR10285:SF139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000545">
        <property type="entry name" value="Pantothenate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00554">
        <property type="entry name" value="panK_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000043236" description="Pantothenate kinase">
        <location>
            <begin position="1"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="96"/>
            <end position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00215"/>
        </source>
    </evidence>
    <sequence length="318" mass="36222" checksum="BE6FAC8572E05F26" modified="2005-10-11" version="1">MDSRADQQQYNPYRVFPRAEWAKLRHDMPMTLAANEIAALRSMHDRLDLVEVEEIYLPLSRLLSIYVAAMQRLFVAQRQFLGIHDRKVPYIIGVAGSVAVGKSTTARVLQALLARWSPRPKVELVTTDGFLLPNAVLERRGLMQKKGFPESYDLPMLLSFLSDIKAGRAPVRAPVYSHLTYDIVPDSWIEVSHPDILIVEGVNVLQTGPLPRDGRAIPVVSDFFDFSVYLDAEEPVLRDWYVTRFLALRDSAFQDPRSYFNRYALLSDDEAIATAIAIWERTNRANLEDNILPTRPRANLILKKGADHVVERVALRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWI9</accession>
    <name>Q3SWI9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Outer membrane lipoprotein carrier protein LolA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0084</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313421.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0084"/>
    <dbReference type="EnsemblBacteria" id="ABA03352">
        <property type="protein sequence ID" value="ABA03352"/>
        <property type="gene ID" value="Nwi_0084"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0084"/>
    <dbReference type="eggNOG" id="COG2834">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055272_0_1_5"/>
    <dbReference type="OMA" id="TQDVYPL"/>
    <dbReference type="OrthoDB" id="1933827at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd16325">
        <property type="entry name" value="LolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029046">
        <property type="entry name" value="LolA/LolB/LppX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004564">
        <property type="entry name" value="OM_lipoprot_carrier_LolA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35869">
        <property type="entry name" value="PTHR35869"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03548">
        <property type="entry name" value="LolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89392">
        <property type="entry name" value="SSF89392"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229202" evidence="2">
        <location>
            <begin position="20"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="258" mass="27824" checksum="E92E502075BF6B00" modified="2005-10-11" version="1">MAGLAAALTASLFVPPALAETVPIPQPAPEVLRAAEPAILLAQAGKLEVTGTTRPPDPIIPDPRRNVPASIFATFDANQKAAAARVSSYLSSLRTLAGKFVQVGPDGSRSTGEFYIQKPGKVRFEYDPPSPIAMISDGSSLVIRDTRLATQDVYPLSQTPLRYLLSDRIDLMRDTNVVAVTSDDLYISVIIEEKQALVGTSRLMFMVGVKDGKLKQWTITDPQGYDTTVAIYNLNPTAKLDPALFRINGTMHPASTNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWL8</accession>
    <name>Q3SWL8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Tryptophan synthase beta chain</fullName>
            <ecNumber evidence="11">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">trpB</name>
        <name evidence="13" type="ordered locus">Nwi_0055</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + H2O + L-tryptophan</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 11">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the TrpB family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="11"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313394.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0055"/>
    <dbReference type="EnsemblBacteria" id="ABA03323">
        <property type="protein sequence ID" value="ABA03323"/>
        <property type="gene ID" value="Nwi_0055"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0055"/>
    <dbReference type="eggNOG" id="COG0133">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016734_3_1_5"/>
    <dbReference type="OMA" id="HGMKSYF"/>
    <dbReference type="OrthoDB" id="912282at2"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06446">
        <property type="entry name" value="Trp-synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00133">
        <property type="entry name" value="Trp_synth_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006653">
        <property type="entry name" value="Trp_synth_b_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006654">
        <property type="entry name" value="Trp_synth_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023026">
        <property type="entry name" value="Trp_synth_beta/beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001413">
        <property type="entry name" value="Trp_syn_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00263">
        <property type="entry name" value="trpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00168">
        <property type="entry name" value="TRP_SYNTHASE_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="10 11 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="PALP" evidence="12">
        <location>
            <begin position="79"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="11">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABA03323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="414" mass="44940" checksum="4932197C2696B775" modified="2005-10-11" version="1">MSSLPNSFRSGPDERGHFGMFGGRFVAETLMPLILDLERAYADAKSDAAFQRDMARYRKDYIGRPSPLYFAERLTDHLREISAASGGAGGAKIYLKREELNHTGSHKVNNVLGQILVARRMGKKRIIAETGAGQHGVATATLCARFGLDCVVYMGAVDVARQEPNVIRMEMLGAKVVPVQSGTRTLKDAMNEALRDWVTNVHDTFYCIGTVAGPHPYPMMVRDFQSIIGEETRRQMQEAEGRLPDSLIACIGGGSNAMGLFHPFLDESSVEIFGVEAAGHGLDKQHAASLTGGRPGVLHGNRTYLLMNGDGQIEDAHSISAGLDYPGIGPEHSWLHETGRVTYLSATDEEALGAFQLLSRLEGIIPALESAHAIARVIELAPGRTKDHLMVVNLSGRGDKDVPQVGDILKGRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWQ4</accession>
    <name>Q3SWQ4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, XRE family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0019</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313358.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0019"/>
    <dbReference type="EnsemblBacteria" id="ABA03287">
        <property type="protein sequence ID" value="ABA03287"/>
        <property type="gene ID" value="Nwi_0019"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0019"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066192_26_0_5"/>
    <dbReference type="OMA" id="EGKARPH"/>
    <dbReference type="OrthoDB" id="2063007at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="17"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="139" mass="15224" checksum="8B88A2E99C4EDC47" modified="2005-10-11" version="1">MSTKAPNPVDKHVGSRVRMRRIMLGMSQEKLGEALGLTFQQVQKYEKGTNRVGASRIQQISEILQVPVSFLFEGSPGTGVVRTGGMSEAPSPAYVSDFLATSEGLALTRAFTRITDAKLRRSIVDMVEQIAAREPSDQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN70</accession>
    <name>Q3SN70_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PMT_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_3021</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3021"/>
    <dbReference type="CAZy" id="GT83">
        <property type="family name" value="Glycosyltransferase Family 83"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06271">
        <property type="protein sequence ID" value="ABA06271"/>
        <property type="gene ID" value="Nwi_3021"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3021"/>
    <dbReference type="eggNOG" id="COG1807">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016165_1_0_5"/>
    <dbReference type="OMA" id="KLTGWTP"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038731">
        <property type="entry name" value="RgtA/B/C-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13231">
        <property type="entry name" value="PMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="182"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="225"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="284"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="307"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="336"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="369"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="PMT_2" evidence="9">
        <location>
            <begin position="82"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA06271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="582" mass="65295" checksum="C63CC8892177B7A3" modified="2005-10-11" version="1">MLEATGARMRDGRNLGIQMIIADPDHPPLHHERPARSSLLWFAALIAAMTAMRCVFAGVLELRTDEAYYWTWSKESALSFLDHPPMIAWFIRFGTAIFGDTNFGVRFAGLLAMPVSQLLLADIVRRVTHDIRAAMLAVLMMEAALYYGLLMAKVAPDIALIPFVLAMVWSLVRLAESGDARWWLAAGVFGGLSLLSKLTAVMLVPAIAAFILVPDWRLRWLRSPYPWAAVLIAVAVFSPVLIWNGMHDWASFRFQFVRATAAHDFNLRTLGDYIGLQFGQVGPILLPVVLFAVGLTAWRGYRHRDPVAVLLSTAVLVPFGYFLWKSLTLRVGDTWPMFMWPLGFAAAAIDIAIRPREKWPAWMVKWSTFWASAAIASGLCLIVVIFLYYIVSPWNFIGKNDPIGGEAGFAQVASRAESEMRNSGATWIATVDYRVYAMLRWHLRDRVPVIQINERGRFLGFRDPGFDRIAGHAGLYVARKPDDSSILASTTAVLTPLGQVQRIWRGRVMDSYVLEKLTGWTPELSPPPESPFYTWRKLAENGAAFRPARAFSGKVDDLFVARNATRKPFLEHGPIQLDRIML</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNA0</accession>
    <name>Q3SNA0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2991</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2991"/>
    <dbReference type="EnsemblBacteria" id="ABA06241">
        <property type="protein sequence ID" value="ABA06241"/>
        <property type="gene ID" value="Nwi_2991"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2991"/>
    <dbReference type="eggNOG" id="ENOG5033HKU">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2383193_0_0_5"/>
    <dbReference type="OMA" id="KCEWDMS"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="114" mass="12952" checksum="2E2F0877EC66BA6F" modified="2005-10-11" version="1">MYCPESCSYDPLNDRERFTQLPVIGAALRPKKCEWDMSKSYIIEVGSESAGIVVRDRAGFRFFSASQRFSSLDGRLFRSARDAERAAKNRALSKKPACPEFVKSLRRALPKLPF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-01-10" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNU7</accession>
    <name>LEUD_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-isopropylmalate dehydratase small subunit</fullName>
            <ecNumber evidence="1">4.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Alpha-IPM isomerase</fullName>
            <shortName evidence="1">IPMI</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Isopropylmalate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">leuD</name>
        <name type="ordered locus">Nwi_2791</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of LeuC and LeuD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LeuD family. LeuD type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315989.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SNU7"/>
    <dbReference type="SMR" id="Q3SNU7"/>
    <dbReference type="STRING" id="323098.Nwi_2791"/>
    <dbReference type="EnsemblBacteria" id="ABA06044">
        <property type="protein sequence ID" value="ABA06044"/>
        <property type="gene ID" value="Nwi_2791"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2791"/>
    <dbReference type="eggNOG" id="COG0066">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081378_0_3_5"/>
    <dbReference type="OMA" id="AFTTHTG"/>
    <dbReference type="OrthoDB" id="1384217at2"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009316">
        <property type="term" value="C:3-isopropylmalate dehydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01577">
        <property type="entry name" value="IPMI_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01031">
        <property type="entry name" value="LeuD_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004431">
        <property type="entry name" value="3-IsopropMal_deHydase_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033940">
        <property type="entry name" value="IPMI_Swivel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00171">
        <property type="entry name" value="leuD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0432">Leucine biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000141846" description="3-isopropylmalate dehydratase small subunit">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01031"/>
        </source>
    </evidence>
    <sequence length="201" mass="22429" checksum="B2C3C8029B693082" modified="2005-10-11" version="1">MDKFTTLEGVAAPLKIINVDTDMIIPKQYLKTIKRTGLGRGLFSEQRYRDDGSENPDFVLNKSAYRNARILVAGDNFGCGSSREHAPWALLDFGIRCVISTSFGDIFYNNCFKNGILPIRVTQADLDKLFDDAERGANATLTIDLANQEIRGPDGGTVKFEIDAFRKHCLLNGLDDIGLTMEKKAAIDSYEDKAKRERAWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP80</accession>
    <name>Q3SP80_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Multisubunit potassium/proton antiporter, PhaF subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_2658</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Cell membrane</location>
            <topology evidence="2 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the CPA3 antiporters (TC 2.A.63) subunit F family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315861.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2658"/>
    <dbReference type="EnsemblBacteria" id="ABA05911">
        <property type="protein sequence ID" value="ABA05911"/>
        <property type="gene ID" value="Nwi_2658"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2658"/>
    <dbReference type="eggNOG" id="COG2212">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125825_1_3_5"/>
    <dbReference type="OMA" id="VIAFARY"/>
    <dbReference type="OrthoDB" id="2083248at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015075">
        <property type="term" value="F:ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007208">
        <property type="entry name" value="MrpF/PhaF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34702">
        <property type="entry name" value="PTHR34702"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04066">
        <property type="entry name" value="MrpF_PhaF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028784">
        <property type="entry name" value="MrpF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0050">Antiport</keyword>
    <keyword evidence="4 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR028784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA05911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="92" mass="9955" checksum="05BE86178C1A8223" modified="2005-10-11" version="1">MSMMLAWSITTAQILLGCAMACAAFRILRGPRAQDRVVGFDSLYVNAMLLVLTLGIRTGTTLYFEAALIIALLGFVSTVALAKFLLRGEVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP97</accession>
    <name>Q3SP97_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transaldolase</fullName>
            <ecNumber evidence="7">2.2.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tal</name>
        <name evidence="9" type="ordered locus">Nwi_2641</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 7">Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>D-glyceraldehyde 3-phosphate + D-sedoheptulose 7-phosphate = beta-D-fructose 6-phosphate + D-erythrose 4-phosphate</text>
            <dbReference type="Rhea" id="RHEA:17053"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:57483"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="2.2.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11816"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="EC" id="5.3.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 7">Carbohydrate degradation; pentose phosphate pathway; D-glyceraldehyde 3-phosphate and beta-D-fructose 6-phosphate from D-ribose 5-phosphate and D-xylulose 5-phosphate (non-oxidative stage): step 2/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the GPI family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 7">Belongs to the transaldolase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.2" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2641"/>
    <dbReference type="EnsemblBacteria" id="ABA05894">
        <property type="protein sequence ID" value="ABA05894"/>
        <property type="gene ID" value="Nwi_2641"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2641"/>
    <dbReference type="eggNOG" id="COG0166">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0176">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013922_0_0_5"/>
    <dbReference type="OMA" id="YKGGPDS"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00181"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00414"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004347">
        <property type="term" value="F:glucose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004801">
        <property type="term" value="F:transaldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05015">
        <property type="entry name" value="SIS_PGI_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00955">
        <property type="entry name" value="Transaldolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00493">
        <property type="entry name" value="Transaldolase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001672">
        <property type="entry name" value="G6P_Isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035476">
        <property type="entry name" value="SIS_PGI_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001585">
        <property type="entry name" value="TAL/FSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004732">
        <property type="entry name" value="Transaldolase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018225">
        <property type="entry name" value="Transaldolase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10683">
        <property type="entry name" value="PTHR10683"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00342">
        <property type="entry name" value="PGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00923">
        <property type="entry name" value="TAL_FSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00662">
        <property type="entry name" value="G6PISOMERASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00876">
        <property type="entry name" value="tal_mycobact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51463">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01054">
        <property type="entry name" value="TRANSALDOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00958">
        <property type="entry name" value="TRANSALDOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="8" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="8 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0570">Pentose shunt</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0704">Schiff base</keyword>
    <keyword evidence="7 9" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="7">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="965" mass="104858" checksum="ABA91147F8A2D997" modified="2005-10-11" version="1">MEFLQTARPRTEKNVMNPVRELQNHGQAIWLDFLARGFITKGDLKTLVDNDGLRGVTSNPSIFEKAIGSSDEYDSAIVSLMKKGDRSVADLYEQLAIEDIQHAADVLRPAYDRLDGADGFVSLEVSPYLANDTEGTIAEARRLWKSVNRKNLMIKVPATPNGLPAIQQLISEGISVNITLLFSQKVYVEVAEAYLAGLQNYIRNGGDPAHVASVASFFVSRIDSAADKQLDEKIAQANDPAEKARLAALKGKVAIANAKLAYQEYKQLFAGEDWQKLEAEGARPQRLLWASTGTKNKDYRDVLYIEELIGPDTVNTVPPATLDGFRDHGELRDSLEENLDDARQVLAALADAGISLDAITRDLVTEGVKLFEDAADKLLGAVARKREKILGSAIDRQTLSLDPSTAKSVEAKADDWRAAGAVRKLWQKDKSVWTNTDEDKWLGWLTSPAAELDRAADYEDYAWRVKKQKFTDAVVLGMGGSSLGPEVLAKTFPKAAGFPKLHVLDSTDPAEIRSLEAAIDIARTVFIVSSKSGGTTEPNALKDYFHARVAKAIGADKAGHRFIAITDPGSSLDAEAARLGYARTFHGDPEIGGRYSILSPFGLVPAATAGIDVTGLLEGALVMVRSCGAQVPPHDNPGVQLGLAMGLAGREGRDKVTILASPKIADFGAWAEQLIAESTGKEGKGLIPVDGEPLGDASLYGDDRFFIDIRTADEEDAAHDEKLRALENAGHPVVRIVMNSIEQIGQEFFRFEIAAAVAGAVLGINPFDQPDVEAAKIRTRELTSAFEKTGRLPDEEPAISANGFDIYTDAENARALREKGADGELSSWLKAHLSRAEPGDYVALLAYVARNEDHIGELQRMRLAVRDRKKVATCAEFGPRFLHSTGQAYKGGPDSGVFLQITADDPRDLKIPGRKMSFSIIKAAQARGDFDVLTRRGRRALRVHLKGNLKSGLSRLDDAISRALM</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP98</accession>
    <name>Q3SP98_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2640</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315843.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2640"/>
    <dbReference type="EnsemblBacteria" id="ABA05893">
        <property type="protein sequence ID" value="ABA05893"/>
        <property type="gene ID" value="Nwi_2640"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2640"/>
    <dbReference type="eggNOG" id="ENOG50349FJ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_163135_0_0_5"/>
    <dbReference type="OMA" id="IVLEFPF"/>
    <dbReference type="OrthoDB" id="2055465at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="85" mass="9705" checksum="4032324CA265A223" modified="2005-10-11" version="1">MPRYFFNTRIGNDLILDPEGEELRDPDHAWRAARKTIREILKSDGEQRAILNAVLEVTDATGEIVLEFPFTEAILNPVARSSTRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPA3</accession>
    <name>Q3SPA3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2635</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315838.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05888">
        <property type="protein sequence ID" value="ABA05888"/>
        <property type="gene ID" value="Nwi_2635"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2635"/>
    <dbReference type="eggNOG" id="COG3169">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133782_0_0_5"/>
    <dbReference type="OMA" id="WYGHLKH"/>
    <dbReference type="OrthoDB" id="1929935at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007437">
        <property type="entry name" value="DUF486"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38482">
        <property type="entry name" value="PTHR38482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04342">
        <property type="entry name" value="DMT_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF021239">
        <property type="entry name" value="UCP021239"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="117" mass="13289" checksum="79DADBDDF456A40B" modified="2005-10-11" version="1">MPIAIPSWLLPILMLFCSNVFMTFAWYGHLKFKESPLPLIVLASWGIAFFEYWLAVPANRWGSAVYSAAELKTIQEVITLVVFAGFSVLYLKQPLGWNHGLGFAFVALGAFFIFHKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPA6</accession>
    <name>Q3SPA6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GCN5-related N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2632</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2632"/>
    <dbReference type="EnsemblBacteria" id="ABA05885">
        <property type="protein sequence ID" value="ABA05885"/>
        <property type="gene ID" value="Nwi_2632"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2632"/>
    <dbReference type="eggNOG" id="COG3153">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081840_0_2_5"/>
    <dbReference type="OMA" id="DPPYYGP"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="12"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="181" mass="19459" checksum="4C18761A6C9428A0" modified="2005-10-11" version="1">MLSAFLMSDLSLTLLTETAGDAQAIERLHERTFGPGRFVLSAYRLREHVDHLLELSLTARIGTLLVGSVRLLPVCIGDTPALLLGPLTVEPPFRGRGIGHALLERALGDAKAKGHRLVVLVGDESYYGRAGFRMIPKGQADMPGPVDRARLLVCELAAGAFEGVSGMIRPDWTFAREAAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPF3</accession>
    <name>Q3SPF3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2585</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315788.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2585"/>
    <dbReference type="EnsemblBacteria" id="ABA05838">
        <property type="protein sequence ID" value="ABA05838"/>
        <property type="gene ID" value="Nwi_2585"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2585"/>
    <dbReference type="eggNOG" id="COG4991">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149985_1_0_5"/>
    <dbReference type="OrthoDB" id="1934491at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009642">
        <property type="entry name" value="DUF1236"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06823">
        <property type="entry name" value="DUF1236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229205" evidence="1">
        <location>
            <begin position="24"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="125" mass="13269" checksum="F21E366316CE6AE1" modified="2005-10-11" version="1">MRNTALAFAALATATGAGLSVHAQGNTIGTAPSTGMVVTQPVEVEAGGGITQEQRRAFRDYVATERVPSFTVSQNIVVGAVLADEGVSLYDVPQRFGATPYRYTVVNGETVLIDPRSRRIMQVID</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="128" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPI6</accession>
    <name>Q3SPI6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Phosphoribosylamine--glycine ligase</fullName>
            <ecNumber evidence="2 7">6.3.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">GARS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Glycinamide ribonucleotide synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Phosphoribosylglycinamide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">purD</name>
        <name evidence="10" type="ordered locus">Nwi_2552</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>5-phospho-beta-D-ribosylamine + ATP + glycine = ADP + H(+) + N(1)-(5-phospho-beta-D-ribosyl)glycinamide + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17453"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the GARS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.13" evidence="2 7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315756.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2552"/>
    <dbReference type="EnsemblBacteria" id="ABA05805">
        <property type="protein sequence ID" value="ABA05805"/>
        <property type="gene ID" value="Nwi_2552"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2552"/>
    <dbReference type="eggNOG" id="COG0151">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027420_3_1_5"/>
    <dbReference type="OMA" id="KATVCKY"/>
    <dbReference type="OrthoDB" id="932854at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00125"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004637">
        <property type="term" value="F:phosphoribosylamine-glycine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00138">
        <property type="entry name" value="GARS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020561">
        <property type="entry name" value="PRibGlycinamid_synth_ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000115">
        <property type="entry name" value="PRibGlycinamide_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020560">
        <property type="entry name" value="PRibGlycinamide_synth_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037123">
        <property type="entry name" value="PRibGlycinamide_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020559">
        <property type="entry name" value="PRibGlycinamide_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020562">
        <property type="entry name" value="PRibGlycinamide_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43472">
        <property type="entry name" value="PTHR43472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01071">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02843">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02844">
        <property type="entry name" value="GARS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01210">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="SSF51246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00877">
        <property type="entry name" value="purD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00184">
        <property type="entry name" value="GARS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="9">
        <location>
            <begin position="107"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA05805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="426" mass="44855" checksum="00235B3EA85C71D4" modified="2005-10-11" version="1">MNILLLGSGGREHALAWKIAGSPLVTKLWCAPGNAGIAREAECVALDIADHAAVIGFCKASKVDLVVVGPEAPLAAGIVDDLAAAGIKAFGPGKAAAQLESSKGFTKDLCRDNGVPTAEYERFRDAGAAKDYIHAHGAPIVIKADGLAAGKGVVVAMTLDEALAAVDMMFEGGFGAAGAEVVVEEFLRGEEASFFALCDGEHAMPLATAQDHKRAFDGDEGPNTGGMGAYSPAPVMTDEMCRRTMDEIIVPTLRAMREKGAPFKGVLFAGLMITEQGPKLIEYNVRFGDPECQVLMLRMRGDIVPALFAACDGQLNHFSMRWFPDPALTVVMAAKGYPGSYAKGTRIDGIDEAAKAGGVEIFHAGTKEEDGRILASGGRVLNVCALGNTVAEAQARAYEAVDRITWPEGFCRRDIGLRAVAREKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPJ1</accession>
    <name>Q3SPJ1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Class I peptide chain release factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2547</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315751.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2547"/>
    <dbReference type="EnsemblBacteria" id="ABA05800">
        <property type="protein sequence ID" value="ABA05800"/>
        <property type="gene ID" value="Nwi_2547"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2547"/>
    <dbReference type="eggNOG" id="COG1186">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089470_3_0_5"/>
    <dbReference type="OMA" id="SEHRSQW"/>
    <dbReference type="OrthoDB" id="2047548at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003747">
        <property type="term" value="F:translation release factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="SSF75620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RF_PROK_I" evidence="3">
        <location>
            <begin position="7"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="103"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="139" mass="15794" checksum="ED609D3A54952301" modified="2005-10-11" version="1">MLWITRTIVIDENDIEISFIRASGPGGQNVNKLSTAAQLRYDTRRLTLAPDIAARLAQLAGQRMTRDGVIVIHAQRFRTQERNRADATDRLIELLRQAAIRPIPRRATKPTLGSKQRRLESKKRRGDVKARRGRAGEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPL7</accession>
    <name>Q3SPL7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Probable multidrug resistance protein NorM</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_2521</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Multidrug efflux pump.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315725.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2521"/>
    <dbReference type="EnsemblBacteria" id="ABA05774">
        <property type="protein sequence ID" value="ABA05774"/>
        <property type="gene ID" value="Nwi_2521"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2521"/>
    <dbReference type="eggNOG" id="COG0534">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012893_6_3_5"/>
    <dbReference type="OMA" id="TGNQKVG"/>
    <dbReference type="OrthoDB" id="1415498at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006603">
        <property type="entry name" value="DinF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="105"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="202"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="247"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="283"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="325"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="367"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="405"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="431"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="471"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="490" mass="52871" checksum="24D8B27109270CA1" modified="2005-10-11" version="1">MLPDARHSASSRDAWLGEFRVTLALGWPLVLTNVAQIALTTTDVIVLGRVGPSALAAGTLGVNLYFAILIFGIGVVTASSPMMAEAFGRKLHTVRDVRRTFRQGMWASVLIAAPSWLLLWHTEAILLLFHQEPRLAADAQVFVRALQWGMLPTLGFIVFRSFFAALERPLSAMMVTAAAILFNVAANWVLVFGHLGLPPLGLLGSGIATTLSNIFLFAGVALVASLGRSFRRYHLFGNWWRADWPRFLALWRVGLPIGAALAFEITVFNAAVFLMGQFGTASIAAHAIAIQIASVSFMVPMGLAQAATVRVGRAYGAEDVDGVTRAGWVSFTLAISFMATTSVLMIAAPRFLISAFVDVNDAANAEVIGLAVSFLTCAAVFQIADGAQVVGAGMLRGLQDTRVPMIYAGLGYWGFGMSLSLLFAFKLGFEGTGIWIGLAAGLVAVAILMMSRWIRRERLGLVPLKELRCREPDPETSGHRPRKRAIHSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPN6</accession>
    <name>Q3SPN6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Purine nucleoside phosphorylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_2502</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>adenosine + H(+) + H2O = inosine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:24408"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:24409"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>adenosine + phosphate = adenine + alpha-D-ribose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:27642"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:27643"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>inosine + phosphate = alpha-D-ribose 1-phosphate + hypoxanthine</text>
            <dbReference type="Rhea" id="RHEA:27646"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:27647"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>phosphate + S-methyl-5'-thioadenosine = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11852"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="EC" id="2.4.2.28"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:11853"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="5 9">Belongs to the purine nucleoside phosphorylase YfiH/LACC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315706.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2502"/>
    <dbReference type="EnsemblBacteria" id="ABA05755">
        <property type="protein sequence ID" value="ABA05755"/>
        <property type="gene ID" value="Nwi_2502"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2502"/>
    <dbReference type="eggNOG" id="COG1496">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065784_2_0_5"/>
    <dbReference type="OMA" id="CFADCVP"/>
    <dbReference type="OrthoDB" id="1393323at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004000">
        <property type="term" value="F:adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017061">
        <property type="term" value="F:S-methyl-5-thioadenosine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd16833">
        <property type="entry name" value="YfiH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.140.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003730">
        <property type="entry name" value="Cu_polyphenol_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038371">
        <property type="entry name" value="Cu_polyphenol_OxRdtase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011324">
        <property type="entry name" value="Cytotoxic_necrot_fac-like_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30616">
        <property type="entry name" value="PTHR30616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02578">
        <property type="entry name" value="Cu-oxidase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64438">
        <property type="entry name" value="SSF64438"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00726">
        <property type="entry name" value="TIGR00726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA05755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="256" mass="27777" checksum="4F45EB1AE541AD15" modified="2005-10-11" version="1">MTLASTLLSTVSGFRHAFFTREGGVSQGIYASLNAGIGSQDDPAHVAENRRRMAEVMEVEPASFLSLYQVHSPDAVIADRAWDVASRPRADAIVTRTPGLAIGVTAADCGPVLFADPRARVIGAAHAGWKGALTGVLEQTIDAMEALGGRRDDIAAAIGPLIRQQSYEVGADFVTRFTEADPGHARFFIPSRHDGHAMFDLGGFIRMRLERAGVRTIDDTGLDTYSSERFFSYRRSVHRKEPDFGRHVHAIVLEAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPT4</accession>
    <name>Q3SPT4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_2454</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NodD regulates the expression of the nodABCFE genes which encode other nodulation proteins. NodD is also a negative regulator of its own expression. Binds flavonoids as inducers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315662.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2454"/>
    <dbReference type="EnsemblBacteria" id="ABA05707">
        <property type="protein sequence ID" value="ABA05707"/>
        <property type="gene ID" value="Nwi_2454"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2454"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_16_2_5"/>
    <dbReference type="OMA" id="EPCRIEP"/>
    <dbReference type="OrthoDB" id="1661500at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0536">Nodulation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="297" mass="33452" checksum="D19D5EA7CAB21824" modified="2005-10-11" version="1">MDWDKLKVFHAAAEAGSFTHAGEQLGLSQSAVSRQVGALEQELSVPLFHRHARGLILTEQGDLLFRTAHQVFMQLQAARAKLADSRERPSGDLKVTTTLGVGINWLIPRLGEFTSLYPDIRISLALTDEELDLSMREADIAIRTYKPTQPDLIQRKLFAICFHAYCSTEYVKRFGTPRTLEELSGHRIILLNETQASPHLQNRNWLIEAARNRSGPHEPYFTVNNIVGLFSACQQGLGIAALPDYLVGENNLLVQLFGESDSIQLDTYFVYPEELKSVARVQVFRDFVVSKAQRWPS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-09-11" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPW7</accession>
    <name>BIOD_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent dethiobiotin synthetase BioD</fullName>
            <ecNumber evidence="1">6.3.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DTB synthetase</fullName>
            <shortName evidence="1">DTBS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Dethiobiotin synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">bioD</name>
        <name type="ordered locus">Nwi_2420</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA, also called 7,8-diammoniononanoate) to form a ureido ring.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(7R,8S)-7,8-diammoniononanoate + ATP + CO2 = (4R,5S)-dethiobiotin + ADP + 3 H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15805"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:149469"/>
            <dbReference type="ChEBI" id="CHEBI:149473"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the dethiobiotin synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.3" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315632.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SPW7"/>
    <dbReference type="SMR" id="Q3SPW7"/>
    <dbReference type="STRING" id="323098.Nwi_2420"/>
    <dbReference type="EnsemblBacteria" id="ABA05674">
        <property type="protein sequence ID" value="ABA05674"/>
        <property type="gene ID" value="Nwi_2420"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2420"/>
    <dbReference type="eggNOG" id="COG0132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072551_2_0_5"/>
    <dbReference type="OMA" id="SPHWAAE"/>
    <dbReference type="OrthoDB" id="1739932at2"/>
    <dbReference type="UniPathway" id="UPA00078">
        <property type="reaction ID" value="UER00161"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004141">
        <property type="term" value="F:dethiobiotin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00336">
        <property type="entry name" value="BioD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004472">
        <property type="entry name" value="DTB_synth_BioD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43210">
        <property type="entry name" value="PTHR43210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006755">
        <property type="entry name" value="DTB_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00347">
        <property type="entry name" value="bioD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0093">Biotin biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000302536" description="ATP-dependent dethiobiotin synthetase BioD">
        <location>
            <begin position="1"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="33"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="13"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="100"/>
            <end position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00336"/>
        </source>
    </evidence>
    <sequence length="213" mass="23168" checksum="759B2A14F34EA687" modified="2005-10-11" version="1">MSARIVVSGTDTGVGKTVFSAALAGALDGYYWKPVQSGLDEETDSQTVMRLSGLGAERVWPEAYRLRTPVSPHLAAELDGVTIDHRALEAPHTDRPLVIEGAGGLLVPLTRDVTYLDLMARWRLPVVLCARTALGTINHSLLSIEALRARDVAILGVSFIGEENVETEGIIIDMGRVKRLGRLSYVTPLTRATLRQAFADGFDIGDFVKERAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPY0</accession>
    <name>Q3SPY0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Helix-turn-helix, Fis-type</fullName>
            <ecNumber evidence="3">2.7.7.49</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1143</name>
        <name evidence="3" type="ordered locus">Nwi_2407</name>
        <name evidence="4" type="ordered locus">Nwi_2433</name>
        <name evidence="5" type="ordered locus">Nwi_2865</name>
        <name evidence="6" type="ordered locus">Nwi_2879</name>
        <name evidence="7" type="ordered locus">Nwi_2941</name>
        <name evidence="8" type="ordered locus">Nwi_3106</name>
    </gene>
    <organism evidence="3 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="3 9" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="3">Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04405">
        <property type="protein sequence ID" value="ABA04405"/>
        <property type="gene ID" value="Nwi_1143"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05661">
        <property type="protein sequence ID" value="ABA05661"/>
        <property type="gene ID" value="Nwi_2407"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05687">
        <property type="protein sequence ID" value="ABA05687"/>
        <property type="gene ID" value="Nwi_2433"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06115">
        <property type="protein sequence ID" value="ABA06115"/>
        <property type="gene ID" value="Nwi_2865"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06129">
        <property type="protein sequence ID" value="ABA06129"/>
        <property type="gene ID" value="Nwi_2879"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06191">
        <property type="protein sequence ID" value="ABA06191"/>
        <property type="gene ID" value="Nwi_2941"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06353">
        <property type="protein sequence ID" value="ABA06353"/>
        <property type="gene ID" value="Nwi_3106"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1143"/>
    <dbReference type="KEGG" id="nwi:Nwi_2407"/>
    <dbReference type="KEGG" id="nwi:Nwi_2433"/>
    <dbReference type="KEGG" id="nwi:Nwi_2865"/>
    <dbReference type="KEGG" id="nwi:Nwi_2879"/>
    <dbReference type="KEGG" id="nwi:Nwi_2941"/>
    <dbReference type="KEGG" id="nwi:Nwi_3106"/>
    <dbReference type="HOGENOM" id="CLU_027402_10_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010921">
        <property type="entry name" value="Trp_repressor/repl_initiator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48295">
        <property type="entry name" value="SSF48295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="132"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA06115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA06129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA06191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA06353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="326" mass="37766" checksum="6D89C889AE8E86FF" modified="2005-10-11" version="1">MRYPASEKAEIIALVEQSHLPAKRTLDKLGIPRATFYRWYDRYRAGGIEALADHRSRPDRVWNRIPDDVRGQIIDLALELPELSPRELAVRFTDERKYFVSEASVYRLLKAHDLITSPAYVVIKAANEFKDKTTAANQLWQTDFTYLKITGWGWYYLSTVLDDFSRYIVAWRLGPTMCASDVTATLDQALAASGLDHVSVRQRPRLLSDNGSSYVADDLATWLRAKDMQHVRGAPYHPQTQGKIERWHQTLKNRILLENYYLPDDLKRQVAAFVEHYNHDRYHESIGNVTPADVYFGRAETILAERHRIKRDTIANRRLQHQLQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPY6</accession>
    <name>Q3SPY6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NAD-dependent epimerase/dehydratase</fullName>
            <ecNumber evidence="3">5.3.3.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2401</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.3.1" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315616.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2401"/>
    <dbReference type="EnsemblBacteria" id="ABA05655">
        <property type="protein sequence ID" value="ABA05655"/>
        <property type="gene ID" value="Nwi_2401"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2401"/>
    <dbReference type="eggNOG" id="COG0451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_863171_0_0_5"/>
    <dbReference type="OMA" id="CARTQFK"/>
    <dbReference type="OrthoDB" id="1928091at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004769">
        <property type="term" value="F:steroid delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="2">
        <location>
            <begin position="4"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="316" mass="35759" checksum="9D87A41A82F396CE" modified="2005-10-11" version="1">MKTMVTGSSGFIGQALVRRLRQEQFEVFGLDNRHSAATDHVYDILDAERLKEVVQKLSPDFLVHLAARTDLDEKHDLAGYATNIDGVKNLIDAVRLTPSIKRAIWTSSQLVCRVGYVPHSDTDYNADTLYGQSKMRTEQIVRADNGADRDWILVRPTTVWGPGMSAHYQRFLRMIQRGHYFHVGTKPLWKSYSYIENIAHQYIQMLRAPTDAVHGKTFYLADYEPLDLLTWGDSFQRAFKARRIPHIPLTVARALALCGDAANAAGLNQVPFNSFRLNNVLTQYTFNMRPTAAVCGPLPYTIEQGVTETAAWLEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ14</accession>
    <name>Q3SQ14_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2373</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2373"/>
    <dbReference type="EnsemblBacteria" id="ABA05627">
        <property type="protein sequence ID" value="ABA05627"/>
        <property type="gene ID" value="Nwi_2373"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2373"/>
    <dbReference type="eggNOG" id="ENOG5031H0G">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1617282_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007825">
        <property type="entry name" value="Major_OMP_Legionella"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05150">
        <property type="entry name" value="Legionella_OMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="164" mass="17665" checksum="79CE1FDD48A7E0E4" modified="2005-10-11" version="1">MRFPAPVDIGLGIPIQSIDNSTTSTLNQRSSFFGAGPRVGVEGFVPLGGGWAFDYLGDAAILFGSQKLTSTSTVTTTISPAFLAILAGGGTNTATTVRDQRFAAVFNTDLQLGVSYWFNPNVRISASYRLDAYFNAFNATLDPNTRDTISRYIHGPRLRVTAQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ67</accession>
    <name>Q3SQ67_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Small multidrug resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_2320</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the drug/metabolite transporter (DMT) superfamily. Small multidrug resistance (SMR) (TC 2.A.7.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315539.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2320"/>
    <dbReference type="EnsemblBacteria" id="ABA05574">
        <property type="protein sequence ID" value="ABA05574"/>
        <property type="gene ID" value="Nwi_2320"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2320"/>
    <dbReference type="eggNOG" id="COG2076">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133067_1_2_5"/>
    <dbReference type="OMA" id="FEIAWAI"/>
    <dbReference type="OrthoDB" id="1990300at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042221">
        <property type="term" value="P:response to chemical"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000390">
        <property type="entry name" value="Small_drug/metabolite_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045324">
        <property type="entry name" value="Small_multidrug_res"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30561">
        <property type="entry name" value="PTHR30561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00893">
        <property type="entry name" value="Multi_Drug_Res"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="33"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="106" mass="11262" checksum="32D2B7C9D0344C3D" modified="2005-10-11" version="1">MSWLYLFLAGLAETGWAIGLKYTDGFTRLWPSVFTGLGMALSFMLLSIAIRTLPVGTAYAIWTGIGATGTALLGMWLFSEPATIARFACLGLIIIGMVGLKVVSPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ90</accession>
    <name>Q3SQ90_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">FecR protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2297</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315518.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2297"/>
    <dbReference type="EnsemblBacteria" id="ABA05551">
        <property type="protein sequence ID" value="ABA05551"/>
        <property type="gene ID" value="Nwi_2297"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2297"/>
    <dbReference type="eggNOG" id="COG3712">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050192_0_1_5"/>
    <dbReference type="OMA" id="WVSVEPA"/>
    <dbReference type="OrthoDB" id="1381035at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006860">
        <property type="entry name" value="FecR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032623">
        <property type="entry name" value="FecR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012373">
        <property type="entry name" value="Ferrdict_sens_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30273">
        <property type="entry name" value="PTHR30273"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16220">
        <property type="entry name" value="DUF4880"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04773">
        <property type="entry name" value="FecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018266">
        <property type="entry name" value="FecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4880" evidence="3">
        <location>
            <begin position="16"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="FecR" evidence="2">
        <location>
            <begin position="122"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="327" mass="35653" checksum="27E6BB387C885A79" modified="2005-10-11" version="1">MVFASDNRDDRERASREATEWFVRLNNPLASDETRRAYRAWLKADPAHHDAMVDISELWGALDRPAARLAAAGWHRSAVASPRRGPLKLGLRFAAAAVIAMVLTGGGAIWRDPGMIDRAFADVATQPGERREVRLADGSLVMLDGGTALKTDMVGGVRAITMLRGRVWFEVVADRQRPFTVHAGRVDVLVHGTAFEVDREAGAVTVEHGKVAVSDAEATRGPVNLTNWQRVSLKDGALGAPEAVDPEQVFAWRRGLIVLDRAPLSRVVKELDNMAAGRVLIIDPELRRMTLSGTFRADDPGAVLEALRSTLGVRTLSVPGFATLIYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQA0</accession>
    <name>Q3SQA0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FAD-dependent pyridine nucleotide-disulfide oxidoreductase</fullName>
            <ecNumber evidence="3">1.18.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2287</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.18.1.1" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315508.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2287"/>
    <dbReference type="EnsemblBacteria" id="ABA05541">
        <property type="protein sequence ID" value="ABA05541"/>
        <property type="gene ID" value="Nwi_2287"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2287"/>
    <dbReference type="eggNOG" id="COG0446">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003291_4_0_5"/>
    <dbReference type="OMA" id="MFRPEKF"/>
    <dbReference type="OrthoDB" id="1149616at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015044">
        <property type="term" value="F:rubredoxin-NAD+ reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028202">
        <property type="entry name" value="Reductase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14759">
        <property type="entry name" value="Reductase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="SSF55424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="1">
        <location>
            <begin position="6"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Reductase_C" evidence="2">
        <location>
            <begin position="321"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="406" mass="43274" checksum="B670F186A56A4C19" modified="2005-10-11" version="1">MGDAPILIVGAGHAGFQLAVSLRQAGYEGPVGLINDEPHLPYQRPPLSKAYLKGGGSPDSVMFRPEKFYHDQHIELIADRAVAIDRGALQLVCASGASRPYKHLVLATGARNRLLDVPNADLEDVLYLRSLDESEKLRQLFTTRRRVVVVGAGFIGLEFAATARSKGLEVDVVELAPRVMARAVTPEISEFFQQRHSEAGIRLHFGVQATSIAGDGGRVSGVALSDGRQLAADLVVVGIGVLPNVELAGEAGLPVASGIIVDEHLLTADPNISAIGDCALFASERFGASLRLESVQNATDQARCVAARLTGHAKNYDGFPWFWSDQGPDKLQIAGLTTGYDQVVVRGDVGQGSFSAFCYKGGHLIGIESVNRASDHVFGRRFLAVNGSITPEQAADSAFDLKRALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQB0</accession>
    <name>Q3SQB0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Amidase</fullName>
            <ecNumber evidence="3">3.5.1.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2277</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the amidase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.4" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315498.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2277"/>
    <dbReference type="EnsemblBacteria" id="ABA05531">
        <property type="protein sequence ID" value="ABA05531"/>
        <property type="gene ID" value="Nwi_2277"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2277"/>
    <dbReference type="eggNOG" id="COG0154">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009600_0_3_5"/>
    <dbReference type="OMA" id="TLINMID"/>
    <dbReference type="OrthoDB" id="1239251at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004040">
        <property type="term" value="F:amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043864">
        <property type="term" value="F:indoleacetamide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="PTHR11895"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="SSF75304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="2">
        <location>
            <begin position="25"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="449" mass="46544" checksum="A81A2B9A0CEA9A7D" modified="2005-10-11" version="1">MPDDPTLATLAADLAAGRTTARALVSDCLAKIADQAGEGARVFIQVDADAAIAAAEAMDRLRGVNAAPSPYAGIPVSIKDLFDVRGQVSRAGSLALKDAPPAAKDAPVVARLRRAGFVVIGRTNMTEFAYSGLGINPHYGTPKNGWRRDVGHVPGGSSSGAAVSITDGMAHGALGTDTGGSCRIPAAYCGIVGYKPTARRIPLDGGVPLSVTLDSYGPIARSVGCCAVLDAVLADEPVTAVAPRPVRGLRFAAPTTVMLDRLDPAVASAFERALDLLADAGALIDHIAVPEFGEVAELNIGGGFAAAESWAWHRALIAEKAELYDPRVLSRIRYGEGLSAADYVDLLSVRRAIIGSFGRRVAPYDGLLSPTVAITPPRIADLADDAAYVKANMLSLRNCSLINILDGCAISLPCHRESEPPVGLMLAGTGGADRRIFELAAAIEPIVRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQI8</accession>
    <name>Q3SQI8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Molybdenum cofactor cytidylyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2199</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315422.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2199"/>
    <dbReference type="EnsemblBacteria" id="ABA05453">
        <property type="protein sequence ID" value="ABA05453"/>
        <property type="gene ID" value="Nwi_2199"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2199"/>
    <dbReference type="eggNOG" id="COG2068">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_505971_0_0_5"/>
    <dbReference type="OMA" id="LNGADWV"/>
    <dbReference type="OrthoDB" id="472474at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012184">
        <property type="entry name" value="Bifunc_Mopterin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036626">
        <property type="entry name" value="MPTBd_MobAlike"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="SSF53218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MoCF_biosynth" evidence="1">
        <location>
            <begin position="169"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="534" mass="55606" checksum="97CDEE5479699DB6" modified="2005-10-11" version="1">MKFGPATPDDALGGVTVHTLRQGAFVLKKGTIIGPMEVEALNRAGVKEIVVVRLEDGDVSEDVAAADVAKAVAGEGVTVERAFTGRANLFAARAGVLVIDRPAVNRINNIDEAITFATLPACKPVVQGEMVATVKLIPFGVEAKLRDAAVAAARRDVLRVLPYRIKRVGIVSTMLPGLAPKVIDKTLRVTAERLAPAGATIIAERRVPHEEAVLSAAIKEMLDLGAELVIVFGASAIADRRDVIPAALTSAGGVVEHFGMPVDPGNLLLIGKARGVPVLGAPGCARSPVENGFDWVLMRLLAGLKVAKRDLTGMGVGGLLMEIVTRPQPRISPAADSNCNIAALILAAGCSTRMGGPNKLLAEVGGKPLLRIVTEQVLASKASSVTVVTGHQAAEVERTLRGLNVTFVYNPDFPEGLASSIRVGIGAIPKEAGGALVCLGDMPLVDADLIDQLIAAFAPDRGQLIAVPVSDGRQGNPVLWSRRFFGELMSLDGDIGGRHLLASHAAAVAEVSVDTHGAFFDIDTPQALAAVQRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQK8</accession>
    <name>Q3SQK8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2179</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2179"/>
    <dbReference type="EnsemblBacteria" id="ABA05433">
        <property type="protein sequence ID" value="ABA05433"/>
        <property type="gene ID" value="Nwi_2179"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2179"/>
    <dbReference type="eggNOG" id="ENOG5031HEF">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1641961_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="161" mass="16625" checksum="2A83C60C0960CF3C" modified="2005-10-11" version="1">MGRSFGARDGGYAATAKGLCGGVAGATARTAVAIKAAVRAEKAGGLVRLAKDVGRISEKTGTRGAFDVLRIAQGPKDVARAARLAKAKGGQTRAIIKILGRGALLLTAGAFNLTMWVFSAALALFGFLSSIRAATERLTLSSLQRSRARRQRRLSAVSSPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQQ5</accession>
    <name>Q3SQQ5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DEAD/DEAH box helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2131</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315357.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2131"/>
    <dbReference type="EnsemblBacteria" id="ABA05386">
        <property type="protein sequence ID" value="ABA05386"/>
        <property type="gene ID" value="Nwi_2131"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2131"/>
    <dbReference type="eggNOG" id="COG1061">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011686_0_0_5"/>
    <dbReference type="OrthoDB" id="259198at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="152"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="2">
        <location>
            <begin position="357"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1099" mass="122165" checksum="2D4EB8588E346B5C" modified="2005-10-11" version="1">MGLFEDWRGTLTLPRLAELRVKVGRNLVRQIVFRGATTRARIFTSEFPGHDLIKTDLKSPYDEIYLRRKGAKRRTTNLPVVTTGLNLDPAKLPGTLTLRWDGITPLVERANTPEKLLDTWVNQFSFRLEGENGEPGLRLPQIGALHAIAAHFAVGDTFQPATVVLPTGTGKTETMLAAQVYLRPARTLVLVSGVPLRDQIEEKFVTLGYLPTAEAVPIELPGPRVAVISGGIRSVAEAENLIKEANLFIALPNSLDASHPDAIDALAAGCSHLFVDEAHHITAKTWKSVRDRFSKEKVVQFTATPFRRDEQRVDGKIIFNYKLGDAQRAGYYKRINLKTVEEYGDQEARDEAVARAAVEALRHDLNDEKLDHILLARTETQARADVLAELYQRLAPEFAPVKVYSDRPDSQNRAALAALRNRNNTGSRIAVCVDMLGEGFDFPQLKVAALHDTHKSLAITLQFIGRFTRKGPVNVGNATVVTNIADPQAERKLAGLYAEGADWDHLIRRLSEARIDDELRLQDVVEQLKQKGTLSAELSLWNLRPAISTQFYKTKCKEWMPLQYADVLPPGAETWYALDDKDKLLVAVVALTEEVKWGDYQNITNSFYDLIIARWDSDESVLSIYASDYDRMRTEQMAKAITSNNVELFGGDAIFNILNGVELPLVKNLGSRRVGAISFTTYFGTNVTEGLAHIDKSEAELNHIACVGYEDGDRVLWGGAKRRGKVWQLRKSGSVADWAAWTKTTWGKVTTDVDDVTNIIKGFLRPIKLAAPHASHAISAEWGEQAQLSLSERQSVLFGTTEKLLYEVDVGIDSVEADGTINVCFEAEDVGAVYRLEISPALPGGYAYKHTSGPKVQFKKVTKPAEPLEVYLQRDPVIIRYADGTHSYNCYHIPTKLDAGIYSKDQLETWDFKGISLNKESIGKAGDTATIQYRAFAQLRDEYDLIFNDDGKAEAGDLVCLKDVDESTIKLTLVHCKGAIGARVSGSIENFYFVCGQAQKCITKKHKGVERLAKDLKRREAQWAANGATRFLKGGQRELSYFKEKSRKSKVEFEVVLVQPGASVDSVTVPILQLLATTELFLKKTTDANFRVVLNAASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQR2</accession>
    <name>Q3SQR2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2124</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315350.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2124"/>
    <dbReference type="EnsemblBacteria" id="ABA05379">
        <property type="protein sequence ID" value="ABA05379"/>
        <property type="gene ID" value="Nwi_2124"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2124"/>
    <dbReference type="eggNOG" id="ENOG50332PM">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2734331_0_0_5"/>
    <dbReference type="OMA" id="ADDGQPW"/>
    <dbReference type="OrthoDB" id="1945484at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="73" mass="8109" checksum="62C7D03E7C142F3D" modified="2005-10-11" version="1">MTKLSSATPRRVVFQYLVPVHVEVEDGLVRSVTVIDETPVENPTVVEGDADYLEEAVKAADDGQPWPSWQFGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQV8</accession>
    <name>Q3SQV8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ABC-type Fe3+-siderophore transport system permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_2075</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315312.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2075"/>
    <dbReference type="EnsemblBacteria" id="ABA05333">
        <property type="protein sequence ID" value="ABA05333"/>
        <property type="gene ID" value="Nwi_2075"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2075"/>
    <dbReference type="eggNOG" id="COG0609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013016_0_2_5"/>
    <dbReference type="OMA" id="AWKRGVH"/>
    <dbReference type="OrthoDB" id="1283908at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000522">
        <property type="entry name" value="ABC_transptr_permease_BtuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472">
        <property type="entry name" value="PTHR30472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01032">
        <property type="entry name" value="FecCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="236"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="280"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="310"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA05333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="333" mass="34654" checksum="B578803760C8CE3C" modified="2005-10-11" version="1">MTRLMFTPVLLLGAILFALTIGRYPLQIGDVVSFFLAMAGLKPMAPESYQLLHNIIVEIRLPRILGAALVGAALASSGAAFQAVFRNPLVSPGILGVLGGASFGAALGILLFGNWALVQLSAFIMGLVAVSVGLMIASMFGQASMILLVLGGMISAALFTSALSIVKYTADPYEELPAIVYWLMGSLGGVDMTQIRWAMIPIVGGLVALSLFGRALDALSMGDDEARALGVPAQPVRYGVIGAATLVSALSVSLAGMIGWVGLVVPHVVRLALGPGNARLLPISAFCGAIFLIGADCLSRSIMRSEIPIGILTELLGIPAFIIVLHRARSGWT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-01-09" modified="2022-08-03" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQZ1</accession>
    <name>MACB_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Macrolide export ATP-binding/permease protein MacB</fullName>
            <ecNumber evidence="1">7.6.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">macB</name>
        <name type="ordered locus">Nwi_2041</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Non-canonical ABC transporter that contains transmembrane domains (TMD), which form a pore in the inner membrane, and an ATP-binding domain (NBD), which is responsible for energy generation. Confers resistance against macrolides.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. Macrolide exporter (TC 3.A.1.122) family.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315283.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SQZ1"/>
    <dbReference type="SMR" id="Q3SQZ1"/>
    <dbReference type="STRING" id="323098.Nwi_2041"/>
    <dbReference type="EnsemblBacteria" id="ABA05300">
        <property type="protein sequence ID" value="ABA05300"/>
        <property type="gene ID" value="Nwi_2041"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2041"/>
    <dbReference type="eggNOG" id="COG0577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_78_2_5"/>
    <dbReference type="OMA" id="VVILITH"/>
    <dbReference type="OrthoDB" id="1181903at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51267">
        <property type="entry name" value="MACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0046">Antibiotic resistance</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000269951" description="Macrolide export ATP-binding/permease protein MacB">
        <location>
            <begin position="1"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="526"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="574"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="596"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="6"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="42"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01720"/>
        </source>
    </evidence>
    <sequence length="645" mass="68174" checksum="D9DA94967BE26A3D" modified="2005-10-11" version="1">MTPPLIELEGIRRSYRSGDVVTHALRGVGLSIHAGEFVAIIGASGSGKSTLMNIIGLMDRPSDGAYRFGGRDVATLNRDELAALRRGCFGFIFQNYHLIPTVSALGNVEMPAIHAGAPRAYRHRRATALLTRLGLANRITNRPSQLSGGQQQRVSIARALMNGGAVILADEPTGALDSKSGTEVLGILKKLAGDGHTVILITHDSKVAAAAERIIRIEDGLIVSDSGPDPEKVSSSIAVVPWQASDSSPPLWTWLEEAARSAFAALAINPVRTALTLSGIVIGVASVVAMMAIGRGAQASYIERASAIGTNWIVVDRAGESTGNSLPLTPADAQAIKDMDNVSGSMPAMWDMATMRRGNIDLNTDVVATTAEFRTVHNWDMAKGTFFTKQDEVSGGPVVLLGATLASKLFPGIADPSGSNILINNLPFLVTGVLESKGLSERGTDRDKRAVMPLRTATMRLFGKDDLSEIVVSIADMSRLHETKEAIKALLIRRHGREDFYIYDSASAFQKAEDERRSSNLLLSAIAAISMLVGGIGIMNIMLITVSERTREIGVRTAIGARTADILGQFLTEAVVLAAIGGVVGLLLGAVIGVGAALLFGMTVIFSVTMALGALMGAVVMGTVFGFMPAYRAARLKPIEALARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQZ5</accession>
    <name>Q3SQZ5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2037</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315280.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2037"/>
    <dbReference type="EnsemblBacteria" id="ABA05296">
        <property type="protein sequence ID" value="ABA05296"/>
        <property type="gene ID" value="Nwi_2037"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2037"/>
    <dbReference type="HOGENOM" id="CLU_1766082_0_0_5"/>
    <dbReference type="OrthoDB" id="2099221at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="156" mass="17615" checksum="575673D14672380E" modified="2005-10-11" version="1">MLTLVKDVDVPVQPAAPPSKPRSRQAPRQRDYNRRRFLGWLRDEVAVLRSVGLGVREIAVRLDIEEYDVLAFCPKPKESVLRRSLPRRAVEALLHGRHASLGGRTIAKRSRHLVEIASAYSWGELLSEPGVGTVTASEIRLWLEERGMTLRKSEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR62</accession>
    <name>Q3SR62_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">FecR protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1969</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1969"/>
    <dbReference type="EnsemblBacteria" id="ABA05229">
        <property type="protein sequence ID" value="ABA05229"/>
        <property type="gene ID" value="Nwi_1969"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1969"/>
    <dbReference type="eggNOG" id="COG3712">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050192_0_1_5"/>
    <dbReference type="OMA" id="HARAWRQ"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006860">
        <property type="entry name" value="FecR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032623">
        <property type="entry name" value="FecR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012373">
        <property type="entry name" value="Ferrdict_sens_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30273">
        <property type="entry name" value="PTHR30273"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16220">
        <property type="entry name" value="DUF4880"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04773">
        <property type="entry name" value="FecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018266">
        <property type="entry name" value="FecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4880" evidence="3">
        <location>
            <begin position="11"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="FecR" evidence="2">
        <location>
            <begin position="104"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="377" mass="41268" checksum="E29317080E90EA85" modified="2005-10-11" version="1">MEQNGTQSVDDQAVQWFVLLRDEDATAEDRRAFAHWLAFDQTHETAWQSVERMWGGLDALAPEVVRQRRKPRRNSRLKRGAAVALLLAAIGIGWQMAPTGLFADYRTAIGERRMIVLQDGSQVDLGAASALDVSFGTGERRVRLLTGEAFFTVTKDAARPFIVAAENGEVKVLGTAFDVKIADGVAVAVAHNAVQVSAQASTQAPDRPDVVPDAPVVVRQGQIVRYDFAGISAVGAADLESIQAWRHGQLVFRDVPLDTVLTELQRYRFGRIVLIGGTLGQRRVTAVFDAAKTEQALDTIAESLSLRIYRAAGLLTVIIPNDSAEKLPRATNYFSRSCRLQNRDGIAPSWPIPCPHVEDGGRPITRWASRANCWTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR65</accession>
    <name>Q3SR65_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RNA polymerase ECF-type sigma factor, possible FecI</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1966</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1966"/>
    <dbReference type="EnsemblBacteria" id="ABA05226">
        <property type="protein sequence ID" value="ABA05226"/>
        <property type="gene ID" value="Nwi_1966"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1966"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3063985_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r4_2" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="53" mass="5977" checksum="F12B424EB22B9FCF" modified="2005-10-11" version="1">MPPRCREVLILHKFESLSYAQIADRLGIAKNSVAAHMVKAISHLRSRFREASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR72</accession>
    <name>Q3SR72_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1959</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1959"/>
    <dbReference type="EnsemblBacteria" id="ABA05219">
        <property type="protein sequence ID" value="ABA05219"/>
        <property type="gene ID" value="Nwi_1959"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1959"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073272_0_0_5"/>
    <dbReference type="OMA" id="ICAIAQT"/>
    <dbReference type="OrthoDB" id="1488434at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="312" mass="34281" checksum="C5F02ACAA307CAE7" modified="2005-10-11" version="1">MPSSIDRKAADLLLSVIETPDAAIRGSVLDGYYGRVAPALKEAGILQPKDHLRAAVSLADHEDEPINLTWSPEHRAYGYFSPTAGWVSVPGDQLATFRVNFSRLLDQLVERLDLSPRSAPVELVPDLLWEVGDARLPGRSKRTPVWIGRRLGDPAIWKRFIDTARKRPAPGLRIVLSFTPGNCLPTDVHLGHTLIAVRDVADHYGLAVVPDLLAARVAAGSQLNDDPITMAADGASLTVRGTRYAFSGSKQRAIIRQLYDAWKSGHPELLTAEVLESAGFSTSVNTLGKAFSGRSDWREFLNEEHGRCWMFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR79</accession>
    <name>Q3SR79_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1952</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1952"/>
    <dbReference type="EnsemblBacteria" id="ABA05212">
        <property type="protein sequence ID" value="ABA05212"/>
        <property type="gene ID" value="Nwi_1952"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1952"/>
    <dbReference type="eggNOG" id="COG3547">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124387_1_0_5"/>
    <dbReference type="OMA" id="WDGVEHT"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021322">
        <property type="entry name" value="DUF2924"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11149">
        <property type="entry name" value="DUF2924"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="169" mass="19358" checksum="C4412E1AF351D5CC" modified="2005-10-11" version="1">MQEATKRTETIPRRPRESTAADENVVAQLAALKRMTVVELKAKWESLLGTPAPNNSRSYLELRLGYRIQELTLSGLSRETRRTLDLLADEIEGRIGRKTIIADPRNPVVGTRLMREWDGVEHTVTVMKDGFDWQGRKFKSLSAVARAITGTQWNGYRFFGLREARRDDR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRB0</accession>
    <name>PDXJ_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyridoxine 5'-phosphate synthase</fullName>
            <shortName evidence="1">PNP synthase</shortName>
            <ecNumber evidence="1">2.6.99.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pdxJ</name>
        <name type="ordered locus">Nwi_1921</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate = H(+) + 2 H2O + phosphate + pyridoxine 5'-phosphate</text>
            <dbReference type="Rhea" id="RHEA:15265"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57279"/>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
            <dbReference type="ChEBI" id="CHEBI:58589"/>
            <dbReference type="EC" id="2.6.99.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homooctamer; tetramer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PNP synthase family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="ABA05181" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.6.99.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05181.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_041345572.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRB0"/>
    <dbReference type="SMR" id="Q3SRB0"/>
    <dbReference type="STRING" id="323098.Nwi_1921"/>
    <dbReference type="EnsemblBacteria" id="ABA05181">
        <property type="protein sequence ID" value="ABA05181"/>
        <property type="gene ID" value="Nwi_1921"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1921"/>
    <dbReference type="eggNOG" id="COG0854">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074563_0_0_5"/>
    <dbReference type="OrthoDB" id="1159993at2"/>
    <dbReference type="UniPathway" id="UPA00244">
        <property type="reaction ID" value="UER00313"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033856">
        <property type="term" value="F:pyridoxine 5'-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008615">
        <property type="term" value="P:pyridoxine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00003">
        <property type="entry name" value="PNPsynthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00279">
        <property type="entry name" value="PdxJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004569">
        <property type="entry name" value="PyrdxlP_synth_PdxJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036130">
        <property type="entry name" value="Pyridoxine-5'_phos_synth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30456">
        <property type="entry name" value="PTHR30456"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03740">
        <property type="entry name" value="PdxJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63892">
        <property type="entry name" value="SSF63892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00559">
        <property type="entry name" value="pdxJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0664">Pyridoxine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000231819" description="Pyridoxine 5'-phosphate synthase">
        <location>
            <begin position="1"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>3-amino-2-oxopropyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57279"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="14"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>1-deoxy-D-xylulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>3-amino-2-oxopropyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57279"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>1-deoxy-D-xylulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>1-deoxy-D-xylulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>1-deoxy-D-xylulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>3-amino-2-oxopropyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57279"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="221"/>
            <end position="222"/>
        </location>
        <ligand>
            <name>3-amino-2-oxopropyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57279"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="256" mass="27307" checksum="F70D433DC0540C7A" modified="2006-04-04" version="2">MSSSQSLRLGVNVDHVATIRNARGGDRPDVVRIALAAIAAGADGITAHLREDRRHIRDSDMARLKAEIEKPLNFEMAATAEMQGIALATQPHAVCLVPERREELTTEGGLDAAGQYNALAPFIAKLNDAGIRVSLFIAADPRQIEAAAKLRAPVIEIHTGGWCDAVVEGDSGKADAEWRRIVEGAALARSVGLEVHAGHGLDYATAETIAALPEITELNIGYFMMGEALLVGIAETVRTMRAAMDRGRATLKTRTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRB2</accession>
    <name>Q3SRB2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">Signal peptidase I</fullName>
            <ecNumber evidence="3 6">3.4.21.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_1919</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.</text>
            <dbReference type="EC" id="3.4.21.89"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <topology evidence="6">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peptidase S26 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.89" evidence="3 6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315175.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1919"/>
    <dbReference type="MEROPS" id="S26.001"/>
    <dbReference type="EnsemblBacteria" id="ABA05179">
        <property type="protein sequence ID" value="ABA05179"/>
        <property type="gene ID" value="Nwi_1919"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1919"/>
    <dbReference type="eggNOG" id="COG0681">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028723_1_2_5"/>
    <dbReference type="OMA" id="SDSRFWG"/>
    <dbReference type="OrthoDB" id="1741894at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000223">
        <property type="entry name" value="Pept_S26A_signal_pept_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019758">
        <property type="entry name" value="Pept_S26A_signal_pept_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019757">
        <property type="entry name" value="Pept_S26A_signal_pept_1_Lys-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43390">
        <property type="entry name" value="PTHR43390"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10502">
        <property type="entry name" value="Peptidase_S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00727">
        <property type="entry name" value="LEADERPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="SSF51306"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02227">
        <property type="entry name" value="sigpep_I_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00760">
        <property type="entry name" value="SPASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00761">
        <property type="entry name" value="SPASE_I_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S26" evidence="7">
        <location>
            <begin position="15"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="44"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR600223-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA05179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="252" mass="28331" checksum="E3E265F6FF16F1C7" modified="2005-10-11" version="1">MSVTSGTKTENGVGETIRVVIHALIIALVIRTFLFQPFNIPSGSMKATLLVGDYLFVSKYSYGYSHYSIPFSPPLFSGRVFGSEPSRGDVVVFRLPKDNATDYIKRVIGLPGDRIQMKEGLLYINDAPVQRERLPDFVGEDPCGSDATARVKQWQETLPNGVTYKTLDCVDNGFYDNTNVYVVPPGHFFMMGDNRDNSTDSRVLSAVGYVPLENLVGRAQMIFFSIAEGEHAWQVWRWPAAVRWNRMFSIVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRC4</accession>
    <name>Q3SRC4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, MarR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1907</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1907"/>
    <dbReference type="EnsemblBacteria" id="ABA05167">
        <property type="protein sequence ID" value="ABA05167"/>
        <property type="gene ID" value="Nwi_1907"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1907"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109241_0_0_5"/>
    <dbReference type="OMA" id="WSDQIRY"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023187">
        <property type="entry name" value="Tscrpt_reg_MarR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13463">
        <property type="entry name" value="HTH_27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01117">
        <property type="entry name" value="HTH_MARR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="24"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="170" mass="19656" checksum="E0231513DF8B7BCD" modified="2005-10-11" version="1">MKAVAKAHEPAEQVAGQAPVQSLYLEALTLVERLHRRLLDVIKDEFDRRGRADINSVQALLLYNIGDKELTAGELRTRGYYLGSNVSYNLKKLVELGFLDHQRSRVDRRSVRIRLTAQGQEIRRIVDALYQKHVKTVEQVGGISNEEFATLNKSLHRLERFWTDQILYRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRE2</accession>
    <name>Q3SRE2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1889</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1889"/>
    <dbReference type="EnsemblBacteria" id="ABA05149">
        <property type="protein sequence ID" value="ABA05149"/>
        <property type="gene ID" value="Nwi_1889"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1889"/>
    <dbReference type="eggNOG" id="ENOG5030CC3">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2207252_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="107" mass="11399" checksum="EC45E65DE80D8BF2" modified="2005-10-11" version="1">MTHKPEKSGPMKPFQKSREAVLKQTGCIALACKPRNAAKRRQTMNHSIQSADRATHVKIVIVALVAGAVLTAFGTSLRPSTDFVQTVQVVKAGQPTMVSSSGASVVR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-09-05" modified="2022-05-25" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRG6</accession>
    <name>SYP_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Proline--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Prolyl-tRNA synthetase</fullName>
            <shortName evidence="1">ProRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">proS</name>
        <name type="ordered locus">Nwi_1865</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro)</text>
            <dbReference type="Rhea" id="RHEA:14305"/>
            <dbReference type="Rhea" id="RHEA-COMP:9700"/>
            <dbReference type="Rhea" id="RHEA-COMP:9702"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78532"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.15"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.15" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315121.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRG6"/>
    <dbReference type="SMR" id="Q3SRG6"/>
    <dbReference type="STRING" id="323098.Nwi_1865"/>
    <dbReference type="EnsemblBacteria" id="ABA05125">
        <property type="protein sequence ID" value="ABA05125"/>
        <property type="gene ID" value="Nwi_1865"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1865"/>
    <dbReference type="eggNOG" id="COG0442">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016739_4_2_5"/>
    <dbReference type="OMA" id="NCDYAAN"/>
    <dbReference type="OrthoDB" id="665824at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004827">
        <property type="term" value="F:proline-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006433">
        <property type="term" value="P:prolyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00861">
        <property type="entry name" value="ProRS_anticodon_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00779">
        <property type="entry name" value="ProRS_core_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01570">
        <property type="entry name" value="Pro_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002316">
        <property type="entry name" value="Pro-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004500">
        <property type="entry name" value="Pro-tRNA-synth_IIa_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023716">
        <property type="entry name" value="Prolyl-tRNA_ligase_IIa_type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044140">
        <property type="entry name" value="ProRS_anticodon_short"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033730">
        <property type="entry name" value="ProRS_core_prok"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01046">
        <property type="entry name" value="TRNASYNTHPRO"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00409">
        <property type="entry name" value="proS_fam_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000248904" description="Proline--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01570"/>
        </source>
    </evidence>
    <sequence length="439" mass="49569" checksum="7D5D0A7E12E1A07D" modified="2005-10-11" version="1">MRLSRFFLPILKETPKEAEIVSHRLMLRAGMIRQEAAGIYAWLPLGLRVLKKIEAIVREEQNRAGAIELLMPTLQLADLWRESGRYDAYGPEMLRIQDRHKRELLYGPTNEEMITEIFRGYIRSYKSLPLNLYHIQWKFRDEQRPRFGVMRGREFLMKDAYSFDIDEAGARRSYNRMFVAYLRTFARMGLKAIPMRAETGPIGGDLSHEFIVLAETGESGVYIDKDVLDLPIPGTEVDYDGDLTPIVRQWTTAYAATEDVHDGPRYEREVPEERRVHTRGIEVGQIFYFGTKYSESMKALVTGTDGVEQPIHGGSYGVGVSRLVGAIIEACHDEAGIKWPEAVAPFTVAILNLKQGASDTDEACERIYRELSARGVDVLYDDTDQRAGAKFATADLIGSPWQVMVGPKGLAEGKVEIKRRGDGSRENVALEDAVARLMA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-03-18" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRH4</accession>
    <name>PYRH_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Uridylate kinase</fullName>
            <shortName evidence="1">UK</shortName>
            <ecNumber evidence="1">2.7.4.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Uridine monophosphate kinase</fullName>
            <shortName evidence="1">UMP kinase</shortName>
            <shortName evidence="1">UMPK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrH</name>
        <name type="ordered locus">Nwi_1857</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible phosphorylation of UMP to UDP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + UMP = ADP + UDP</text>
            <dbReference type="Rhea" id="RHEA:24400"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.22"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Inhibited by UTP.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; UDP from UMP (UMPK route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UMP kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.22" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315113.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRH4"/>
    <dbReference type="SMR" id="Q3SRH4"/>
    <dbReference type="STRING" id="323098.Nwi_1857"/>
    <dbReference type="EnsemblBacteria" id="ABA05117">
        <property type="protein sequence ID" value="ABA05117"/>
        <property type="gene ID" value="Nwi_1857"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1857"/>
    <dbReference type="eggNOG" id="COG0528">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033861_0_0_5"/>
    <dbReference type="OMA" id="PIIVFDM"/>
    <dbReference type="OrthoDB" id="1043239at2"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00275"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033862">
        <property type="term" value="F:UMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04254">
        <property type="entry name" value="AAK_UMPK-PyrH-Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01220_B">
        <property type="entry name" value="PyrH_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011817">
        <property type="entry name" value="Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015963">
        <property type="entry name" value="Uridylate_kinase_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005650">
        <property type="entry name" value="Uridylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02075">
        <property type="entry name" value="pyrH_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000323903" description="Uridylate kinase">
        <location>
            <begin position="1"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="12"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="135"/>
            <end position="142"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01220"/>
        </source>
    </evidence>
    <sequence length="238" mass="25075" checksum="E3CEE02515F959D3" modified="2005-10-11" version="1">MDRPAYRRVVVKLSGEYLAGSQPFGIDQPTIDRIAGDLAEARALGVEIAVVVGGGNIFRGVEVSSRGVSRPTGDTMGMLATVMNCLALEAALERRGQSARALSSFVMPEVCELFTRRAAHKYLAEDRIVLLAGGTGNPYFTTDTTAVLRAAEIGAQAVLKATNVDGVYSSDPKKDPSAKRFERLSHSQALEGGYKVMDATAFALARETALPIIVFSIAEPGSIGAILKGTGRGTIVAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRM6</accession>
    <name>Q3SRM6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Condensin subunit ScpA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1804</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315061.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1804"/>
    <dbReference type="EnsemblBacteria" id="ABA05065">
        <property type="protein sequence ID" value="ABA05065"/>
        <property type="gene ID" value="Nwi_1804"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1804"/>
    <dbReference type="eggNOG" id="COG1354">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038686_2_0_5"/>
    <dbReference type="OMA" id="PIYMRRG"/>
    <dbReference type="OrthoDB" id="959677at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003768">
        <property type="entry name" value="ScpA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33969">
        <property type="entry name" value="PTHR33969"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02616">
        <property type="entry name" value="SMC_ScpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="278" mass="30818" checksum="CA6754BC4F5EF751" modified="2005-10-11" version="1">MSAEILSFETGRPTERVVADEVALVVDVEGYEGPLDLLLALARQQKVDLSKISILALADQYLVFVEAARKIRLELAADYLVMAAWLAFLKSRLLLPEPPTPDGPSAGEMAAALANRLRRLEAIREASNRLMNRQQLGRDIFPRGNPESIAEIRHPKFTATLFDLLSAYAVQRQQRVLTTVHMAKRTVWSLSEARASLERLVGLAGEDWGCLDEYLMTFVVDPAQRATVFASSFASALELVREGVMDLNQKEAFAPLYFRKRAPQPSPDPTAGPDTPVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="139" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRN5</accession>
    <name>Q3SRN5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Queuine tRNA-ribosyltransferase</fullName>
            <ecNumber evidence="4">2.4.2.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Guanine insertion enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">tRNA-guanine transglycosylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">tgt</name>
        <name evidence="6" type="ordered locus">Nwi_1795</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>7-aminomethyl-7-carbaguanine + guanosine(34) in tRNA = 7-aminomethyl-7-carbaguanosine(34) in tRNA + guanine</text>
            <dbReference type="Rhea" id="RHEA:24104"/>
            <dbReference type="Rhea" id="RHEA-COMP:10341"/>
            <dbReference type="Rhea" id="RHEA-COMP:10342"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:58703"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:82833"/>
            <dbReference type="EC" id="2.4.2.29"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">tRNA modification; tRNA-queuosine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer. Within each dimer, one monomer is responsible for RNA recognition and catalysis, while the other monomer binds to the replacement base PreQ1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the queuine tRNA-ribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.29" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315052.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1795"/>
    <dbReference type="EnsemblBacteria" id="ABA05056">
        <property type="protein sequence ID" value="ABA05056"/>
        <property type="gene ID" value="Nwi_1795"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1795"/>
    <dbReference type="eggNOG" id="COG0343">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022060_0_1_5"/>
    <dbReference type="OMA" id="GIDLFDC"/>
    <dbReference type="OrthoDB" id="1165356at2"/>
    <dbReference type="UniPathway" id="UPA00392"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008479">
        <property type="term" value="F:queuine tRNA-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008616">
        <property type="term" value="P:queuosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101030">
        <property type="term" value="P:tRNA-guanine transglycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.105">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00168">
        <property type="entry name" value="Q_tRNA_Tgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004803">
        <property type="entry name" value="TGT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002616">
        <property type="entry name" value="tRNA_ribo_trans-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01702">
        <property type="entry name" value="TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="SSF51713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00430">
        <property type="entry name" value="Q_tRNA_tgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00449">
        <property type="entry name" value="tgt_general"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0671">Queuosine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TGT" evidence="5">
        <location>
            <begin position="16"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="4">
        <location>
            <begin position="93"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="RNA binding" evidence="4">
        <location>
            <begin position="249"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="RNA binding; important for wobble base 34 recognition" evidence="4">
        <location>
            <begin position="273"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="268"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="377" mass="41497" checksum="80406F939B312246" modified="2005-10-11" version="1">MSLANHFELLGRDGDARTGLLTTAHGTVRTPAFMPVGTQGAMKGLQWQDVRDAGADIVLGNTYHLMLRPGAERIHELGGLQKFTRWGGPMLTDSGGFQVMSLAQLRKVKEQGVTFRSHIDGTAIELTPERAIEVQRLLGSDIAMQLDECVRLPAERGDIERAMQLSLRWAERSRRAFEGAPAANMLFGIVQGGDIPELRRTSACGLTGIGFHGYAIGGLAVGEPQATMLAMIEEAAPWLPMERPRYLMGVGTPHDLLEAVARGIDMFDCVMPTRNGRHGMAFTRFGQINLRNARHADDPRPLDEESSWPAARDYSRAYLHHLVRSGETLGAMLLSGINIAYYQSLMQAMRSAIAGGVFQEFRERTREGWARGDIASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRU2</accession>
    <name>Q3SRU2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alkyl hydroperoxide reductase C</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Peroxiredoxin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Thioredoxin peroxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Nwi_1738</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peroxiredoxin family. Prx6 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314995.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1738"/>
    <dbReference type="EnsemblBacteria" id="ABA04999">
        <property type="protein sequence ID" value="ABA04999"/>
        <property type="gene ID" value="Nwi_1738"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1738"/>
    <dbReference type="eggNOG" id="COG0450">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_4_2_5"/>
    <dbReference type="OMA" id="MIDYQDT"/>
    <dbReference type="OrthoDB" id="892697at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03016">
        <property type="entry name" value="PRX_1cys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019479">
        <property type="entry name" value="Peroxiredoxin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045020">
        <property type="entry name" value="PRX_1cys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10417">
        <property type="entry name" value="1-cysPrx_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="5 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 11" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="10">
        <location>
            <begin position="3"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate" evidence="9">
        <location>
            <position position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA04999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="219" mass="24471" checksum="A22B522979FB4E2C" modified="2005-10-11" version="1">MTLSIGSTAPDFEAESTEGPISFHKWLGDSWGLFFSHPKDFTPVCTTELGAVARLKPEFDKRGVKLIGISVDPTDKHHLWSKDIEETQGVAPNYPLIADVDFKVAKLYGMLPASTSGDVNKRTPADNQTVRNIFVIGPDKQVKIVLIYPMSTGRNWQEVLRVIDSLQLTAKHGVSTPSDWKQGDDVFFSAAVPEEEARKKFPKGWKSPKPYFRIVEQPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRU7</accession>
    <name>Q3SRU7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">FecR protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1733</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314990.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1733"/>
    <dbReference type="EnsemblBacteria" id="ABA04994">
        <property type="protein sequence ID" value="ABA04994"/>
        <property type="gene ID" value="Nwi_1733"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1733"/>
    <dbReference type="eggNOG" id="COG3712">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050192_0_1_5"/>
    <dbReference type="OMA" id="IMIEDSA"/>
    <dbReference type="OrthoDB" id="1381035at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006860">
        <property type="entry name" value="FecR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032623">
        <property type="entry name" value="FecR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012373">
        <property type="entry name" value="Ferrdict_sens_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30273">
        <property type="entry name" value="PTHR30273"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16220">
        <property type="entry name" value="DUF4880"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04773">
        <property type="entry name" value="FecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018266">
        <property type="entry name" value="FecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4880" evidence="3">
        <location>
            <begin position="13"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="FecR" evidence="2">
        <location>
            <begin position="110"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="319" mass="33784" checksum="D0F43B892DF7965E" modified="2005-10-11" version="1">MTEAPAEDRRLFREAADLAIRLQNDPANPVSVDMVRTWVARSPRHEAAWARVAAIHGMTGRVLTEQRKAAEGGIGGMSRRRLVIGGLIGAGVAAAGSLTVPGALLRARADYMTSTAEIRRITLSDGSEITLGPDSAVAIDFADARRTIGLLAGMAYFDVVRDASRPFSVLSGDLTATALGTAFDVSNDAGFVSVSVNHGAVEARAAHSALRLAERLNPGDWITFDTSSHAIARGTREASQIAAWREGMIVAERETVSAMVAKIARWQPGRVVMADPFLGSRVVSGVFDLADPLGALEAVVRPFGAKVRVTPFMTVISPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRZ6</accession>
    <name>Q3SRZ6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1684</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314944.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1684"/>
    <dbReference type="EnsemblBacteria" id="ABA04945">
        <property type="protein sequence ID" value="ABA04945"/>
        <property type="gene ID" value="Nwi_1684"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1684"/>
    <dbReference type="eggNOG" id="COG1561">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076609_0_1_5"/>
    <dbReference type="OMA" id="EIKSVNH"/>
    <dbReference type="OrthoDB" id="1509828at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005229">
        <property type="entry name" value="CHP00255"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013551">
        <property type="entry name" value="DUF1732"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013527">
        <property type="entry name" value="YicC-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30636">
        <property type="entry name" value="PTHR30636"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08340">
        <property type="entry name" value="DUF1732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03755">
        <property type="entry name" value="YicC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00255">
        <property type="entry name" value="TIGR00255"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YicC_N" evidence="1">
        <location>
            <begin position="4"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1732" evidence="2">
        <location>
            <begin position="211"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="295" mass="32560" checksum="61B96EBF794FBFA3" modified="2005-10-11" version="1">MALSSMTGFARSHGASGPYAFEWELKSVNAKGFDLRTRLPSGWDDIDVTIRKRATQVLSRGTVYANLTVKRSNAAPVVRINESVLASLLNTAQELRNKIDALAPSIDGLLSIKGVLEVVEPEPDEAEEQAVKLAAADSFEQALRSLADMREREGAELGRILMQRIDEIERLASRADAAPGRRPGAVMARLSEQIAALLDTSDRFDQDRLHQEALLIAAKADIREELDRIASHVSQTRELIGNGGPIGRKLDFLAQEFNREVNTCCSKSNDLELTNVGLELKNVVEQFREQVQNLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS26</accession>
    <name>Q3SS26_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glyoxalase/bleomycin resistance protein/dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1654</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314914.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1654"/>
    <dbReference type="EnsemblBacteria" id="ABA04915">
        <property type="protein sequence ID" value="ABA04915"/>
        <property type="gene ID" value="Nwi_1654"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1654"/>
    <dbReference type="eggNOG" id="COG2764">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046006_11_1_5"/>
    <dbReference type="OMA" id="FTEHAVY"/>
    <dbReference type="OrthoDB" id="1467120at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="136" mass="14902" checksum="D1F7E9E320EFF2EB" modified="2005-10-11" version="1">MGSTVIPAMRYRDPHKMIDWLCETFGFARKSVHDDGNGGVAHAELTLGEGMIMLGSARDDDFAKVQKPATTDHLVTQSAYIVVPDADAVYSRAKANGAIIVLEISDKDYGGREFTCRDPEGQVWSFGTYSPWAQSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS45</accession>
    <name>Q3SS45_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1635</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314895.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04896">
        <property type="protein sequence ID" value="ABA04896"/>
        <property type="gene ID" value="Nwi_1635"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1635"/>
    <dbReference type="HOGENOM" id="CLU_2001459_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229095" evidence="1">
        <location>
            <begin position="26"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="124" mass="13438" checksum="40ABA111F05CC07A" modified="2005-10-11" version="1">MRYSNSLRATILAVTIGSLGMSAHAGPMPTYAAPTMKSVIQKNTTDVRWGGAGYGVAGVRVRGAIASGAYYGGYGGYYYPAYGYRAVSYYDDSYCLRPLVVTPATRIQSRYLGVKPSERLPWTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS94</accession>
    <name>Q3SS94_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1586</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314853.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1586"/>
    <dbReference type="EnsemblBacteria" id="ABA04847">
        <property type="protein sequence ID" value="ABA04847"/>
        <property type="gene ID" value="Nwi_1586"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1586"/>
    <dbReference type="eggNOG" id="COG0262">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1451842_0_0_5"/>
    <dbReference type="OMA" id="ALFFDRY"/>
    <dbReference type="OrthoDB" id="1624280at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="SSF53597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="182" mass="19798" checksum="0E17ACA29E248E18" modified="2005-10-11" version="1">MLRIEGYVIVSADGMLADANRVMPEALKFKGDLEFFTSGLDDAAVVVHGRNSFEDQPNSPLRKRIILTHRVAGVEPDPENEKATLWNPAGASFEDACSHAGVRDGTAAIIGGPTVFEMFLDRYDTFWLSEAPHVRLPGGEGCFPAVPDSSPQAVLTAHGLQPREVRILDAKNDVGVTAWRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSE4</accession>
    <name>Q3SSE4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1536</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04797">
        <property type="protein sequence ID" value="ABA04797"/>
        <property type="gene ID" value="Nwi_1536"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1536"/>
    <dbReference type="HOGENOM" id="CLU_2233677_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="105" mass="10942" checksum="8D8077FA7BE80767" modified="2005-10-11" version="1">MPGRSDPVPERPAVPPTGGPFLRAPSALTNPSDLRSIDGAVGNLSGRYRSTAAPNRAAGHVRLARAGRTTASHGDQRRHGQRDQDGTHHAPALGSTIVNPAIERG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSG9</accession>
    <name>Q3SSG9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">AMP-dependent synthetase and ligase</fullName>
            <ecNumber evidence="2">6.2.1.26</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1511</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.26" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314778.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1511"/>
    <dbReference type="EnsemblBacteria" id="ABA04772">
        <property type="protein sequence ID" value="ABA04772"/>
        <property type="gene ID" value="Nwi_1511"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1511"/>
    <dbReference type="eggNOG" id="COG0318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_534072_0_0_5"/>
    <dbReference type="OMA" id="AVGGYRF"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008756">
        <property type="term" value="F:o-succinylbenzoate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="16"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="500" mass="53224" checksum="D98B91C86926B59A" modified="2005-10-11" version="1">MLQNNSSPTLDELFDRILVRQPDRPALIDPPDKLRVTGQSPLHLTFAQADRAVSALAAHFIEAGLPANSIIAVQLPNTIELALTVLAAHRAGLIVALLPQLWRQAELAVAINRTGARAIVAMGNIDGVNHADLAMNAAAEVFSVRHVCGFGSNLPEGMVPLDIATERTIDRSRLMIRDSRRAALVTFEASGSGFLAVPRTHVNLIAAGLAIFLESSLPQNATILSALAPSSFAGLTSSFVMWLLSGGTLAMHHPFDETVLVRQMETERCDTLVAPASLALRMAETGALSSVPSLRHVVGLWRVPEQVGSSAPWSDSERQMTDVYLFGEAGLFASRRVEDGSPAPIMPGPYRTPPQVPITSTAGEIVLTPHGTLGLRGPMVPIAAYAAPESPSEDFVVPSQPDYVDTGYAARIDRATGAIAITAPPAGVMAVGGYRFPAQDLQEWARRLGPNAMLTALPDRLSGHRLAGRAGDNARAREALKELGLNPLMVEAFRDRSVSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSH6</accession>
    <name>Q3SSH6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zinc-containing alcohol dehydrogenase superfamily</fullName>
            <ecNumber evidence="2">1.6.5.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1504</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.6.5.5" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314771.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1504"/>
    <dbReference type="EnsemblBacteria" id="ABA04765">
        <property type="protein sequence ID" value="ABA04765"/>
        <property type="gene ID" value="Nwi_1504"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1504"/>
    <dbReference type="eggNOG" id="COG0604">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_3_1_5"/>
    <dbReference type="OMA" id="VWYLLHK"/>
    <dbReference type="OrthoDB" id="1225778at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003960">
        <property type="term" value="F:NADPH:quinone reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002364">
        <property type="entry name" value="Quin_OxRdtase/zeta-crystal_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01162">
        <property type="entry name" value="QOR_ZETA_CRYSTAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PKS_ER" evidence="1">
        <location>
            <begin position="11"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="324" mass="34910" checksum="59D49E90F330EA22" modified="2005-10-11" version="1">MTRAVRVHRVGGPEALVYEAVDLGTPGQGEVRIRQHAIGLNFIDVYYRSGLYKTPAAPFIVGNEAAGEVVSVGPGVTNFHPGDRVAYYFDLGGYATDRNIPADKLVKLPDHITYEQGAVLMLKGLTVWYLLHKTFKVEHGHRVLIHAAAGGIGLLACQWAKALGANVIGTVGSKAKADLALANGCDHVILYEEEDFVARVKQISRNELCDVVYDGVGKMTFPGSLSCLRPRGLFVSFGNASGRVPPFPLVELSHHGSLFATRPKLNDYIATRGELLEGADTVFAAVITGSLHVQINHAYALKDAAKAQTDLESRRTTGAIILKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSH9</accession>
    <name>Q3SSH9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AHS1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_1501</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314768.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1501"/>
    <dbReference type="EnsemblBacteria" id="ABA04762">
        <property type="protein sequence ID" value="ABA04762"/>
        <property type="gene ID" value="Nwi_1501"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1501"/>
    <dbReference type="eggNOG" id="COG2049">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020207_1_0_5"/>
    <dbReference type="OMA" id="MPWMLAA"/>
    <dbReference type="OrthoDB" id="1848526at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003833">
        <property type="entry name" value="CT_C_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010016">
        <property type="entry name" value="KipI_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34698">
        <property type="entry name" value="PTHR34698"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02682">
        <property type="entry name" value="CT_C_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00796">
        <property type="entry name" value="AHS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="SSF50891"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00370">
        <property type="entry name" value="TIGR00370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AHS1" evidence="4">
        <location>
            <begin position="9"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="244" mass="26595" checksum="D423EDC3B14E16BD" modified="2005-10-11" version="1">MAAPSLAPPRLLPSGDTAITVEFCRIIDELANHRVLALDRALAEEPIEGVTETVPTYRSLLVHYDPVRIGFEELAERLSALARLPVAPATRARRWRIPVAYGGEHGIDLEDVAKTLNMTPDDIVARHAGGDYRVAMIGFTPGWSYLSGLDSSLRIPRLQSPRLLTPSGMISIGGLQAGIQCLGGPSGWRLLGRTAVRTYQLHRDPVFLLEPGDAITFYAVDGKQFAEQERAAAHGEWVAQLVTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSM8</accession>
    <name>Q3SSM8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MazG nucleotide pyrophosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1452</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314720.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1452"/>
    <dbReference type="EnsemblBacteria" id="ABA04713">
        <property type="protein sequence ID" value="ABA04713"/>
        <property type="gene ID" value="Nwi_1452"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1452"/>
    <dbReference type="eggNOG" id="COG3956">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038356_0_1_5"/>
    <dbReference type="OMA" id="HPHIYGD"/>
    <dbReference type="OrthoDB" id="1099129at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004518">
        <property type="entry name" value="MazG_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011551">
        <property type="entry name" value="NTP_PyrPHydrolase_MazG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30522">
        <property type="entry name" value="PTHR30522"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03819">
        <property type="entry name" value="MazG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00444">
        <property type="entry name" value="mazG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MazG" evidence="1">
        <location>
            <begin position="31"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="MazG" evidence="1">
        <location>
            <begin position="177"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="274" mass="30595" checksum="465F316E20991BB2" modified="2005-10-11" version="1">MTTPSRDISRLLDIMKQLRTPGTGCPWDLEQTFATIAPYTIEEAYEVADAIARNDLVDLCDELGDLLLQVVFHARIAEERNAFSFGDVVEAITRKMIRRHPHVFPDDAGYIAPSDVKGTWERIKAEEKAERAARKPMRETPASGLLADIKAGQPALTRAMALQRKASTVGFDWNDPRAVLDKIREEADEIEAALDRGDRSEIASETGDLMFALVNLARHVDADPETALRATNAKFERRFAHIEHALAAKGRSLEDASLAEMDALWNEAKQKETR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSR0</accession>
    <name>Q3SSR0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">O-acetylhomoserine sulfhydrolase</fullName>
            <ecNumber evidence="5">2.5.1.49</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_1420</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.49" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314690.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1420"/>
    <dbReference type="EnsemblBacteria" id="ABA04681">
        <property type="protein sequence ID" value="ABA04681"/>
        <property type="gene ID" value="Nwi_1420"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1420"/>
    <dbReference type="eggNOG" id="COG2873">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018986_4_0_5"/>
    <dbReference type="OMA" id="TYTLFAH"/>
    <dbReference type="OrthoDB" id="637281at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003961">
        <property type="term" value="F:O-acetylhomoserine aminocarboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006235">
        <property type="entry name" value="OAc-hSer/O-AcSer_sulfhydrylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01326">
        <property type="entry name" value="OAH_OAS_sulfhy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="3">
        <location>
            <position position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="425" mass="45175" checksum="6E413A783C9D5000" modified="2005-10-11" version="1">MTDRTPGFATLAVHAGAQPDPTTGARATPIYQTTSFVFNDADHAASLFGLQAFGNIYTRIGNPTNAVLEERVAALEGGTAALAVASGHAAQVITLQQLMKPGDEVIAARKLYGGTINQFTHAFKSFGWNVAWADPDDIDTFEQAVTPRTRAIFIESVANPRGAITDIEAIAAIARKAGVPLIVDNTMASPYLIRPIEHGADIVLHSLTKFLGGHGNSLGGIIVDAGTFDWSKDNKYPILSEPRPEYNGIRLQETFGNFAFAIACRALGLRDLGSALSPFNAFMILTGIETLPLRMQKHCENTQAIAEWLAAHPAVAAVNYAGLPGDPYNALARKYSPKGAGAVFTFSLKGGYDAGVKLVSKLKLFSHLANIGDTRSLVIHPASTTHSQLDDAAKIKAGAAPDIVRLAIGIEDKEDLMADLEQAMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SST8</accession>
    <name>Q3SST8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Replication-associated recombination protein A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_1392</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent ATPase that plays important roles in cellular responses to stalled DNA replication processes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family. RarA/MGS1/WRNIP1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314664.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1392"/>
    <dbReference type="EnsemblBacteria" id="ABA04653">
        <property type="protein sequence ID" value="ABA04653"/>
        <property type="gene ID" value="Nwi_1392"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1392"/>
    <dbReference type="eggNOG" id="COG2256">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017985_0_3_5"/>
    <dbReference type="OMA" id="RIILSQC"/>
    <dbReference type="OrthoDB" id="977094at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032423">
        <property type="entry name" value="AAA_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021886">
        <property type="entry name" value="MgsA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16193">
        <property type="entry name" value="AAA_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12002">
        <property type="entry name" value="MgsA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="SSF48019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="6">
        <location>
            <begin position="59"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA04653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="443" mass="48612" checksum="E3E3E4C50C2074EA" modified="2005-10-11" version="1">MSPKRQHDSRSLFAAAGLERDAPHPLPDRLRPQMLSDVVGQDHILGPDGALTRMLETRTLGSLIFWGPPGTGKTTVARLLADATALHFEQLSAVFSGVNDLKKAFEAARARRESGTGTLLFVDEVHRFNRAQQDSFLPVMEDGTVVLVGATTENPSFELNAALLSRARVLVFRSLDALAVEKLFVRAEEVEGKPLPLDAEARAALVRMADGDGRAALTLVEEVWRSAREGEVFDAGQLQGVLQRRAPIYDKSADGHYNLISALHKSVRGSDPDAALYYLARMIEAGEDPLFLARRVVRMAVEDIGLADPQALVICNAAKDAYDFLGSPEGELAIAQAVIYLATAPKSNAAYKAFGAAMRVAKDAGSLLPPKHILNAPTKLMKSEGYGGGYQYDHDMPDAFSGQDYFPETLGRQTFYDPPDRGFEREIRKRMDYWAKLRRERAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSU6</accession>
    <name>Q3SSU6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein translocase subunit SecY</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">secY</name>
        <name evidence="11" type="ordered locus">Nwi_1384</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Sec protein translocase complex. Heterotrimer consisting of SecY, SecE and SecG subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. Interacts with SecDF, and other proteins may be involved. Interacts with SecA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell inner membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the SecY/SEC61-alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314658.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1384"/>
    <dbReference type="EnsemblBacteria" id="ABA04645">
        <property type="protein sequence ID" value="ABA04645"/>
        <property type="gene ID" value="Nwi_1384"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1384"/>
    <dbReference type="eggNOG" id="COG0201">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030313_0_2_5"/>
    <dbReference type="OMA" id="FAMWLGE"/>
    <dbReference type="OrthoDB" id="1567535at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043952">
        <property type="term" value="P:protein transport by the Sec complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01465">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026593">
        <property type="entry name" value="SecY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002208">
        <property type="entry name" value="SecY/SEC61-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030659">
        <property type="entry name" value="SecY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023201">
        <property type="entry name" value="SecY_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10906">
        <property type="entry name" value="PTHR10906"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00344">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004557">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103491">
        <property type="entry name" value="SSF103491"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00967">
        <property type="entry name" value="3a0501s007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00756">
        <property type="entry name" value="SECY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="214"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="316"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABA04645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="443" mass="48370" checksum="8818253A44AAF146" modified="2005-10-11" version="1">MVSAAEQLAANLNFSAFSKADELKKRIWFTLGALLVYRLGTYIPLPGIDPTVWQKVFNSQAGGILGMFNMFSGGGIDRMAIFALNIMPYISAAIILQLLTSVSPKLEALKKEGEAGRKVINQYTRYLTVVLAFFQSYGIAVGLQGAGNVVTNPGMFFLLSTAITLTGGTMFLMWLGEQITSRGIGNGISLIIMAGIVAELPSALASMLELGRQGALSTGIILIVLIMVVAVIAFIVFMERAQRRLLIQYPKRQVGNKMFDGQSSHLPLKLNTSGVIPPIFASSLLLLPTTVANFNAGSGPEWFQWINTQLGHGRPMFLILYIALIVFFTFFYTAIVFNPTETADNLKKHGGFIPGIRPGERTAEYIDYVLSRITVPGAVYLAVVCLIPEILISYAAVPFYFGGTSLLIVVSVTMDTVSQVQGYLLAHQYEGLIKKSKLRGRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST60</accession>
    <name>Q3ST60_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04531">
        <property type="protein sequence ID" value="ABA04531"/>
        <property type="gene ID" value="Nwi_1270"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1270"/>
    <dbReference type="eggNOG" id="ENOG5030VR7">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120961_0_0_5"/>
    <dbReference type="OMA" id="ATWLMMA"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="137" mass="14935" checksum="78775C1D2AC992C2" modified="2005-10-11" version="1">MIARRADTKARADFATWKMMVKLNGASSLPTEALTFLASYKSLAETMAETEASEATIELIYKSYYVDMGGTGTAPDVKSMPKEPIVDTSNVMAFKRPAPQPAPSVNSAPGARPRLPVALIFICLVLIYVGIRYFLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST74</accession>
    <name>Q3ST74_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1256</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1256"/>
    <dbReference type="EnsemblBacteria" id="ABA04517">
        <property type="protein sequence ID" value="ABA04517"/>
        <property type="gene ID" value="Nwi_1256"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1256"/>
    <dbReference type="HOGENOM" id="CLU_2570361_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="81" mass="9242" checksum="7DCACA662E071624" modified="2005-10-11" version="1">MLGDSNRIGASQVDQSPEAVLRVFRAQGLHATPRYSRRSYWPIWPKTQWIPDSGGFPKRNPVAGGVPRFARQHRIFARKDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST92</accession>
    <name>Q3ST92_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">OmpA/MotB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_1237</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MotB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314528.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1237"/>
    <dbReference type="EnsemblBacteria" id="ABA04499">
        <property type="protein sequence ID" value="ABA04499"/>
        <property type="gene ID" value="Nwi_1237"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1237"/>
    <dbReference type="eggNOG" id="COG1360">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016890_3_1_5"/>
    <dbReference type="OMA" id="QDMPELT"/>
    <dbReference type="OrthoDB" id="1619550at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07185">
        <property type="entry name" value="OmpA_C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025713">
        <property type="entry name" value="MotB_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006665">
        <property type="entry name" value="OmpA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036737">
        <property type="entry name" value="OmpA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13677">
        <property type="entry name" value="MotB_plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00691">
        <property type="entry name" value="OmpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103088">
        <property type="entry name" value="SSF103088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51123">
        <property type="entry name" value="OMPA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="OmpA-like" evidence="7">
        <location>
            <begin position="148"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA04499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="276" mass="30525" checksum="E352B92639905D26" modified="2005-10-11" version="1">MAKKKRGDAHGGGHGWFVTFADLMGLMMSFFVMLVAFSTMDNQKLKIVAGSMRDAFGSQTEVRYSGIIESDGLPTRPKLKNAAHVPPDETSTTPTPNEQERFKIAGARLKTDREFALASASLRQALQDMPELTELSKHIMFEETKEGLNLEIVDQDGRSMFAEGSKEPYERTRMLIRRLAAPLKATPFRISVTGHTASNFIAMRSDYDGFDLSADRANVVRQILEREGVPAGHIFAVSGKSDSMPLFPDDPSLAANRRVTITLMREAPPLPPNLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STA3</accession>
    <name>Q3STA3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1226</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314517.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1226"/>
    <dbReference type="EnsemblBacteria" id="ABA04488">
        <property type="protein sequence ID" value="ABA04488"/>
        <property type="gene ID" value="Nwi_1226"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1226"/>
    <dbReference type="eggNOG" id="ENOG5032SCW">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_747692_0_0_5"/>
    <dbReference type="OMA" id="NNCGPQY"/>
    <dbReference type="OrthoDB" id="1349030at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021293">
        <property type="entry name" value="DUF2865"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11064">
        <property type="entry name" value="DUF2865"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229298" evidence="2">
        <location>
            <begin position="29"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="301"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="339"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="363" mass="39008" checksum="4A4692399D44229A" modified="2005-10-11" version="1">MPDLLFARTMRILACAVGIAGAAADARAQGGPPGTNPICPRLEAQLASIDRGGGDPARAGQIRRYEEAAGRQQAELERVIAHANRMGCDSSGFFQLFNAQSAQCGPITHQIQRMRTNLEQITTSLERLRAGSAGPDRDSQRRSVLIALAQNNCGPQYAAAARNQGSFFDDLFGNHSENPTILPPVDSGAQSGTYRTVCVRSCDGFYFPISFATVPSRFPDDERTCKNLCPATEAALYAYRNPGEDMNQAVSTNGQPYSSLPNAFRYRQEFNPSCSCRAAGQTWAEALKGLDDKATAAQQGDIIVTEDGARKMSQPTRDRDSQKKDSAATTDARRSPPADATPTASTPSNKPIRSVGPTFIPAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STD5</accession>
    <name>Q3STD5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cytochrome c-type biogenesis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_1194</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Possible subunit of a heme lyase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the CcmH/CycL/Ccl2/NrfF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1194"/>
    <dbReference type="EnsemblBacteria" id="ABA04456">
        <property type="protein sequence ID" value="ABA04456"/>
        <property type="gene ID" value="Nwi_1194"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1194"/>
    <dbReference type="eggNOG" id="COG3088">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107187_2_0_5"/>
    <dbReference type="OMA" id="MIAADMR"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005616">
        <property type="entry name" value="CcmH/CycL/Ccl2/NrfF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038297">
        <property type="entry name" value="CcmH/CycL/NrfF/Ccl2_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03918">
        <property type="entry name" value="CcmH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5011020766" description="Cytochrome c-type biogenesis protein" evidence="5">
        <location>
            <begin position="42"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA04456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="187" mass="20504" checksum="82E085F53DB42207" modified="2005-10-11" version="1">MRELVQTLSSLCGRCMAWNRFLPVLGAILCFGLLAVSSAHAVQPDEVMTDPAQEARARDLSRGLRCMVCQNQSIDDSSAPLARDLRLLVRERIAAGDSNQQVLDFLVARYGDYVLLKPPFETSTLPLWLLTPLLLAGGGLALWGYSRRRSKAVKAAQEAPPRLSEEEEARLAKLVAIERAAGEESKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STE5</accession>
    <name>Q3STE5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1184</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314475.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1184"/>
    <dbReference type="EnsemblBacteria" id="ABA04446">
        <property type="protein sequence ID" value="ABA04446"/>
        <property type="gene ID" value="Nwi_1184"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1184"/>
    <dbReference type="eggNOG" id="ENOG5030YFH">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2602461_0_0_5"/>
    <dbReference type="OMA" id="AWGLYVK"/>
    <dbReference type="OrthoDB" id="2147066at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="79" mass="8158" checksum="C36F363FED790DE9" modified="2005-10-11" version="1">MTSDSVWQIVRYLLIAAGSFATGKGWVTADQVTSIIGAIGTLFTVAWGLYVKADTKAVRSATAARPDVPTVSAATGAVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STH3</accession>
    <name>Q3STH3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ISSpo8, transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1156</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314448.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1156"/>
    <dbReference type="EnsemblBacteria" id="ABA04418">
        <property type="protein sequence ID" value="ABA04418"/>
        <property type="gene ID" value="Nwi_1156"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1156"/>
    <dbReference type="eggNOG" id="COG3677">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044348_1_2_5"/>
    <dbReference type="OMA" id="CPRCVAT"/>
    <dbReference type="OrthoDB" id="1667004at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024445">
        <property type="entry name" value="Tnp_ISXO2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024442">
        <property type="entry name" value="Transposase_Zn_ribbon"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12762">
        <property type="entry name" value="DDE_Tnp_IS1595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12760">
        <property type="entry name" value="Zn_Tnp_IS1595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01126">
        <property type="entry name" value="DDE_Tnp_IS1595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE_Tnp_IS1595" evidence="1">
        <location>
            <begin position="133"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="312" mass="35097" checksum="612F692AF44FAA9C" modified="2005-10-11" version="1">MTKAVFQNPMFHDEDKAREALEAVRWPDGAICPHCGNSDQDKLAKIEGQKKSHRPGLYYCNECKGQFTVTVGTVFERSKVPLTKWWMAAHMMNSNKNGVSAHEIHRALGVTYKTAWFMMHRLREAMVVSSGTPMGGSGSAIQADETYWGNTSKRSKSYRKGLKRKAAIVALVDAETGEARTFHVRHGVSASEVREILVTNVDRKSTLVTDESRLYTITGGEFGDHQTMNHSGREYVNAAGFTTNNVENFFGVFKRGMRGTYTFCGEQHLQRYLTEFQFRYNNRSGLGISDGERTAKALKGIEGKRLTYRPTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STK0</accession>
    <name>Q3STK0_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alginate_exp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1129</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1129"/>
    <dbReference type="EnsemblBacteria" id="ABA04391">
        <property type="protein sequence ID" value="ABA04391"/>
        <property type="gene ID" value="Nwi_1129"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1129"/>
    <dbReference type="eggNOG" id="COG3637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040635_0_0_5"/>
    <dbReference type="OMA" id="PNSYTRF"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025388">
        <property type="entry name" value="Alginate_export_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13372">
        <property type="entry name" value="Alginate_exp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229235" description="Alginate_exp domain-containing protein" evidence="2">
        <location>
            <begin position="34"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="Alginate_exp" evidence="3">
        <location>
            <begin position="129"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="514" mass="57303" checksum="D85B8528EA2F4E33" modified="2005-10-11" version="1">MSLMKSVVFDMTARLVLLSAIPLVAIASSPAQAQPRSHAEVQAQGDPSHTNGRNGGTQPQPEIWPKFKPSTSKPLFSLYDALGRPDNFTIRGTFRSRAEGVANAFRPAPFPRDEFANMLFGTIFAEYDTGHNVKIGGEILDARSYFQKNNSVVSALDVNAFELTQAYLNFDLSDVTGDGSKSSVTAGRFTKDVGSRRYMSRQQYRNNTNAYTGVSYDWKGANKDRWTVFWTMPHIRLPDDAQGVRDNAVEYDRESPDLQFYGSSYTFADVFGGSLELFGYGLYEQDSGTSLLNSMQTRNRRLFTPGARLFRGAKPGRFDYEFEGVYQAGLARATTAVTDTRDLDVSAYFVHAETGYTFAVPWLPRLSLIYDHASGDNGNPSTFNRFDSLFGVRREYNAPSLYGPMTRANLITPAVRLYVTPSSIWDAFVTYRAIWLDSATDVFGATQLRDRSGQSGRFAGHQIEARLRYWLIPDAILLETGAAYLVKGRFLRDAPNAPDGGDTIYGYLATQIFF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STT0</accession>
    <name>MURD_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UDP-N-acetylmuramoylalanine--D-glutamate ligase</fullName>
            <ecNumber evidence="1">6.3.2.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">D-glutamic acid-adding enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">murD</name>
        <name type="ordered locus">Nwi_1049</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + D-glutamate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine = ADP + H(+) + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate</text>
            <dbReference type="Rhea" id="RHEA:16429"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29986"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83898"/>
            <dbReference type="ChEBI" id="CHEBI:83900"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MurCDEF family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.9" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314345.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3STT0"/>
    <dbReference type="SMR" id="Q3STT0"/>
    <dbReference type="STRING" id="323098.Nwi_1049"/>
    <dbReference type="EnsemblBacteria" id="ABA04311">
        <property type="protein sequence ID" value="ABA04311"/>
        <property type="gene ID" value="Nwi_1049"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1049"/>
    <dbReference type="eggNOG" id="COG0771">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032540_3_0_5"/>
    <dbReference type="OMA" id="CNFENYL"/>
    <dbReference type="OrthoDB" id="1093223at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008764">
        <property type="term" value="F:UDP-N-acetylmuramoylalanine-D-glutamate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00639">
        <property type="entry name" value="MurD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018109">
        <property type="entry name" value="Folylpolyglutamate_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005762">
        <property type="entry name" value="MurD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01087">
        <property type="entry name" value="murD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0133">Cell shape</keyword>
    <keyword id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000257207" description="UDP-N-acetylmuramoylalanine--D-glutamate ligase">
        <location>
            <begin position="1"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="121"/>
            <end position="127"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00639"/>
        </source>
    </evidence>
    <sequence length="466" mass="48759" checksum="3DB827EF429A69F8" modified="2005-10-11" version="1">MIPVTSFSGKTVAVFGLGGSGLASCHALKAGGAEVIAGDDNADNLAKAAQAGFITADLRTVVWANLAALVLAPGVPLTHPSPHWSVLAARQAGVEVIGDVELFCRERARHAPDAPFIAITGTNGKSTTTALVAHLMREAGYDVQMGGNIGTAILSLEPPRRGRVHVIEMSSYQIDLAPSLDPTVGILLNLSPDHLDRHGTIEHYAQVKERLIAGVQPQGTAVVGVDDHWCAAIADRQEQAGRSVVRVSVKRPLAHGVSVEQDRIVLVSGGARSEIADIGGIGSLRGRHNAQNAACASAGALALGVSRDILQQDLRSFPGLAHRMEQVGRKAHVLFVNDSKGTNADATEKALSSFDEIFWIAGGKPKSGGIASLDAFFPRIRKAYLIGEAAQEFAATLEGKVAYEISGTLEAAVPAAARDAEGAGRAGAVVLLSPACASFDQFRNFEVRGDRFRELVQALPGVTPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STU8</accession>
    <name>Q3STU8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1031</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314327.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1031"/>
    <dbReference type="EnsemblBacteria" id="ABA04293">
        <property type="protein sequence ID" value="ABA04293"/>
        <property type="gene ID" value="Nwi_1031"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1031"/>
    <dbReference type="eggNOG" id="COG3448">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040681_9_1_5"/>
    <dbReference type="OMA" id="RDDYNAY"/>
    <dbReference type="OrthoDB" id="2014094at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="13"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="86"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="143" mass="16415" checksum="8EC49D4422911975" modified="2005-10-11" version="1">MHQFLEQTVDRYMTRDVRTVSCDLTMDKLNDLFASDDFNAYPVVDDQREAIGLVTKFDFLKCFAITLSSVVPHYDELMRRAVSDVMIHEFIYVGPTTKLARVLQLMVEHRLRSIPVMGGGRNLVGIISREDVMRALRDSTRGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU52</accession>
    <name>Q3SU52_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0927</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314230.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0927"/>
    <dbReference type="EnsemblBacteria" id="ABA04189">
        <property type="protein sequence ID" value="ABA04189"/>
        <property type="gene ID" value="Nwi_0927"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0927"/>
    <dbReference type="eggNOG" id="COG3686">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110778_2_1_5"/>
    <dbReference type="OMA" id="IVRAAYV"/>
    <dbReference type="OrthoDB" id="2049059at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.550">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023352">
        <property type="entry name" value="MAPEG-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001129">
        <property type="entry name" value="Membr-assoc_MAPEG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01124">
        <property type="entry name" value="MAPEG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161084">
        <property type="entry name" value="SSF161084"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="84"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="132" mass="14948" checksum="F99E68F1C6CC249D" modified="2005-10-11" version="1">MTIAEWCVFGAVILYLLTIVPFKALRLPRFDNARPRDPEFYGDPLRARALGAHLNGIEAFPFFAAAVLLAEFRDAPQRLIDELAILYLIVRAAYVVTYIGDRPTLRSILWGVGFAINTTIFFLPAIRHWLPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU67</accession>
    <name>Q3SU67_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF2147 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0912</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314215.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0912"/>
    <dbReference type="EnsemblBacteria" id="ABA04174">
        <property type="protein sequence ID" value="ABA04174"/>
        <property type="gene ID" value="Nwi_0912"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0912"/>
    <dbReference type="eggNOG" id="COG4731">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108869_1_2_5"/>
    <dbReference type="OMA" id="HIYNADD"/>
    <dbReference type="OrthoDB" id="1848240at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019223">
        <property type="entry name" value="DUF2147"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09917">
        <property type="entry name" value="DUF2147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229274" description="DUF2147 domain-containing protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2147" evidence="2">
        <location>
            <begin position="29"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="145" mass="15120" checksum="D04ADA2FE7742D51" modified="2005-10-11" version="1">MLVRSGFVIAVLAAMLSVADAQTAADPTGVWLTQAGDAKVRVSRCGGGLCGVIVWLRNPIDPATGRPAVDDKNHNPALSRRPMIGLPLFRGMSPTGPNKWSGSIYNADDGNTYASNVSVAGPGALRVEGCVGALCSGEVWTRSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SU73</accession>
    <name>Q3SU73_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cupin region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0906</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314209.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0906"/>
    <dbReference type="EnsemblBacteria" id="ABA04168">
        <property type="protein sequence ID" value="ABA04168"/>
        <property type="gene ID" value="Nwi_0906"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0906"/>
    <dbReference type="eggNOG" id="COG4101">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090057_0_0_5"/>
    <dbReference type="OMA" id="HTWYGDR"/>
    <dbReference type="OrthoDB" id="1928636at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017102">
        <property type="entry name" value="UCP037087"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037087">
        <property type="entry name" value="UCP037087"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin_2" evidence="1">
        <location>
            <begin position="46"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="142" mass="15123" checksum="B2C678C7527CE5C7" modified="2005-10-11" version="1">MSGLSDAVRVIEPGKTYVGKQGFTYGAGVSKETVGAKNICLNVLPMPPGARAKVHYHDGIETIAYLLEGECAVYYGDKLEHRIAVKAGDHVYMPEDVPHAPCNESGAPCKWLVAHSSGSDQDGIVLLPELDAVLARKSNMSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUC2</accession>
    <name>Q3SUC2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0855</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0855"/>
    <dbReference type="EnsemblBacteria" id="ABA04119">
        <property type="protein sequence ID" value="ABA04119"/>
        <property type="gene ID" value="Nwi_0855"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0855"/>
    <dbReference type="eggNOG" id="COG3843">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1265846_0_0_5"/>
    <dbReference type="OMA" id="THIGQHI"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021795">
        <property type="entry name" value="DUF3363"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11843">
        <property type="entry name" value="DUF3363"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="218" mass="23564" checksum="3FB78641B8350B4C" modified="2005-10-11" version="1">MIGTAYAVVDGIDGRTHHIKLPDIESSGDSAPGSIVELRSFEDAKGNRRVALAARSDLSIEAQTTASGATWLDRQLVARDPADLAGGFGLEVKQAMEARAEHLVREGMARRDGERILFNRNLIGTIRQRELDVLGNRLATETGAPFQKYADGEFVAGAYSRRFALASGRFAMIDDGLGFKLVPWTPSIETHIGQHISGVGRTDGGVDWSFGRKRGLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUG0</accession>
    <name>Q3SUG0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ParB-like nuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0816</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314129.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0816"/>
    <dbReference type="EnsemblBacteria" id="ABA04081">
        <property type="protein sequence ID" value="ABA04081"/>
        <property type="gene ID" value="Nwi_0816"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0816"/>
    <dbReference type="eggNOG" id="COG1475">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1123610_0_0_5"/>
    <dbReference type="OMA" id="KGWWQDR"/>
    <dbReference type="OrthoDB" id="1636318at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB/Sulfiredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02195">
        <property type="entry name" value="ParBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00470">
        <property type="entry name" value="ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="SSF110849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ParB" evidence="2">
        <location>
            <begin position="5"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="247" mass="26384" checksum="CEC58DADE8C424AD" modified="2005-10-11" version="1">MLAIESRPIARLIPYVRNARTHSGDQIAQIAGSIAEFGFVNPVLIGADDVIIAGHGRVLAAEKLGLAEVPVIVLSHLSESQRRALVIADNRIAENAAWDDAMLKAEIAALHEDAFDLDLLGFAEEELGRLLDGLDADAGGDGAADGEPSSDASTETQRATLAERFGIPPFSVLDARKGWWQDRKRAWIDLGIRSELGRGEGATYGVADGVTEPGLNHYRNRNNAAPGGSPRPLDRGWTGKKDKPAHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUG7</accession>
    <name>Q3SUG7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0809</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314122.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0809"/>
    <dbReference type="EnsemblBacteria" id="ABA04074">
        <property type="protein sequence ID" value="ABA04074"/>
        <property type="gene ID" value="Nwi_0809"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0809"/>
    <dbReference type="eggNOG" id="ENOG5032V0G">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_200209_1_1_5"/>
    <dbReference type="OMA" id="KTVEYRT"/>
    <dbReference type="OrthoDB" id="2067185at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="69" mass="7534" checksum="13070A1B3278917E" modified="2005-10-11" version="1">MPWTQTELDALKRAFAGGTLRVTYDGKTVEYGSADDLLKRIRTIETEIASTSGNPRPIAGFASFGRGDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUI4</accession>
    <name>Q3SUI4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Gene transfer agent (GTA) like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0792</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314105.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0792"/>
    <dbReference type="EnsemblBacteria" id="ABA04057">
        <property type="protein sequence ID" value="ABA04057"/>
        <property type="gene ID" value="Nwi_0792"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0792"/>
    <dbReference type="eggNOG" id="COG3391">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007148_0_0_5"/>
    <dbReference type="OMA" id="IAGNIIW"/>
    <dbReference type="OrthoDB" id="646292at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025195">
        <property type="entry name" value="GTA_TIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032876">
        <property type="entry name" value="J_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13547">
        <property type="entry name" value="GTA_TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13550">
        <property type="entry name" value="Phage-tail_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229320" evidence="2">
        <location>
            <begin position="20"/>
            <end position="1313"/>
        </location>
    </feature>
    <feature type="domain" description="GTA_TIM" evidence="3">
        <location>
            <begin position="442"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="domain" description="Phage-tail_3" evidence="4">
        <location>
            <begin position="802"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="1313" mass="140783" checksum="01A41CC1721F0C5E" modified="2005-10-11" version="1">MAVLLLTAAASALTAGASAFVQIAAAAVATAAGGYIDNLLFGPKLEDQQGPRLDSLQVQASTEGAPIPEIAGRVRIAGQIIWATNFKEVATTEKQGGKGGGGGSVKTTTYAYFANFAVGLCEGPIDRIGRIWADGKPLSMAGITMRVYRGTASQAPDPLIEGVEAADNAPAYRGTAYVVFDNLALEKFGNRLPQLTFEVFRRVSSSDGDSVETMVRAVTMIPGAGERAYDPKVQKRNLGGGATTPENDSAGRSTSDWSVALDDLKASLPNVDTVFLVVGWFGDDLRCGSCTIRPKVEVADKVTTPDSWMVHGVARYGAAVISLNAGRPSYGGTPSDDTVVRAIRDLKARGYAVVFYPFVFMDVPVGNALPNPYGGTGQPAYPWRGRITCHPAAGQPGTVDKTATAGTQVASFFGSCLPSHVSVSVNGSTDAVATSYSGPAEWSFRRFIFHYARLCAAVNAIDTGAVDAFLIGSEMRALVSVRDSATNFPAVARLMTLAADVKGIVGGGVKVSYAADWSDYNGYRPTDGSNDVFFHLDPLWADSNIDFVGIDWYAPLADWRDGSGHLDRQAGASSIYDRAYLQSNIEGGEFFDWFYASDAARNAQTRTTITDGAYGKPWVFRSKDLRNWWLNRHYDRPGGVENGSPTAWLAEMKPIWLCELGVPSVDKGANQPNVFYDPKSSESFFPYFSKGTRDDLIQRRAIEAVIGWWEMAAGHNPISGVYGGRMIGTIGVWTWDARPYPAWPGRADLWSDGDLYPLGHWLNGKVGLADLAALVAERCRRVGFTAYDVSTLVGVVTGYLRDRPMSPRAEIEALASAFAFDAVETDGLIRFIPRGRNSVATLTLPELAIPDQGEREEITLTRGQETELPNEVAIGFTDAIDDYKPGAVSANRLAGYSERKSDIRLALVLDQVQAQSIADRALVEAWIERETAGFALPPARIALDPGDVVDLVINGRPRAFRLKRVTERGARDVEAVRAEAAIYTPPLNGIAPPTLTPPPIYGAAVLRLMDLPLLRDSDDGFSPYAAASAAPWGGVVVMDSATGSDFTLDTTLAVRATLGETIQPFPAGPTEYWDEASVLEVKLYAGELASAMPEAILTGSTNSLAIGTPDGDWEIVQFADAVLTAPQTYRLTKLLRGRLGTEHAMRPPLALGAPVVLLNEAVLKIDGKPAERLAARFYRWGPPALDIADPAWQQTTYATKAAGRMPWSPVQITGARNGGGDLAITWVRRTRFGGVWADGVDVPLNEESERYEVDVMNGANVVRTIAVITPTATAGYTAAQQVADFGSAQSSITIRVYQLSAVVGRGWPGVATL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUM9</accession>
    <name>Q3SUM9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA-3-methyladenine glycosidase II</fullName>
            <ecNumber evidence="2">3.2.2.21</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0747</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.2.2.21" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314066.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0747"/>
    <dbReference type="EnsemblBacteria" id="ABA04012">
        <property type="protein sequence ID" value="ABA04012"/>
        <property type="gene ID" value="Nwi_0747"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0747"/>
    <dbReference type="eggNOG" id="COG0122">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_72_5_5"/>
    <dbReference type="OMA" id="RTVAAWY"/>
    <dbReference type="OrthoDB" id="1781735at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008725">
        <property type="term" value="F:DNA-3-methyladenine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052822">
        <property type="term" value="F:DNA-3-methylguanine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052821">
        <property type="term" value="F:DNA-7-methyladenine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043916">
        <property type="term" value="F:DNA-7-methylguanine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENDO3c" evidence="1">
        <location>
            <begin position="52"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="217" mass="23748" checksum="A7EC2942DF26CFF1" modified="2005-10-11" version="1">MTVRLETQSDLDDAVGALVKQDSRLKPILARTGLPALRRREPGFEGLAAIVCGQQLSTASAGAIWGRLSAAFAPFHHDAIRKARVDRLARLGLSAAKIATLKLLAREISASRLNLDVLTEEDADAAHATLVRYRGIGPWTADVYLLFCLGHGDAWPAGDVALQEAIRAGFGLKTRPTPKQMMPLAEPWRPLRGAAAHLWWAYYRAIKRREGIVPQTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUQ4</accession>
    <name>Q3SUQ4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glutathione S-transferase</fullName>
            <ecNumber evidence="3">2.5.1.18</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0721</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.5.1.18" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314041.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0721"/>
    <dbReference type="EnsemblBacteria" id="ABA03987">
        <property type="protein sequence ID" value="ABA03987"/>
        <property type="gene ID" value="Nwi_0721"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0721"/>
    <dbReference type="eggNOG" id="COG0625">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011226_6_1_5"/>
    <dbReference type="OMA" id="LWHKETA"/>
    <dbReference type="OrthoDB" id="1246633at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14497">
        <property type="entry name" value="GST_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="SSF47616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="2">
        <location>
            <begin position="88"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="208" mass="22928" checksum="D65ADF0661BE6328" modified="2005-10-11" version="1">MPTLYTMPGTCSLSPNIAVAWLDAPVEIHNIPYGDQKKEYYLAINSRGQVPALRFEDGDVLTEAAAILAWLGATHGKESHAPGSRLGRKEAEALAYMTSEVHAAYGGHFNTQKFAESAAAQEEVKRKTYEKLAGHYERLNGVLSENGGEWYLGQRSFADTFLYVLTRWIEKTPLSIGDYPALKAFRACMEADEGVKQALARQAMEPIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUR5</accession>
    <name>Q3SUR5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0710</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314030.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA03976">
        <property type="protein sequence ID" value="ABA03976"/>
        <property type="gene ID" value="Nwi_0710"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0710"/>
    <dbReference type="eggNOG" id="ENOG5033ZZE">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1119256_0_0_5"/>
    <dbReference type="OMA" id="VIHDSPP"/>
    <dbReference type="OrthoDB" id="1511354at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="248" mass="26959" checksum="73F63BDBF09B4246" modified="2005-10-11" version="1">MCDYSLHHVSSRPAKVADKLVTMELAKSNVRGFAAVGELGPKLVIHDSPPELAVCLLPGTELAFDENVQYEQPRSFLGIKFSGKARVDHKVASFRKVDEDNPYVQHDALEFPNGQVLKIAQLVAGQTATVLQLPVNAQEDDHDHEHAIRRRARESRLALNRSTRQGMFVPNQGAVMLWDSVCGRAADFSSALTESFQRLTAPKQPSILEGEMAAGMGAEVSFENGVPTVTSTAASQKPEKKVRAGSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUS7</accession>
    <name>Q3SUS7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aminotransferase</fullName>
            <ecNumber evidence="2">2.6.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0698</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.6.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314020.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0698"/>
    <dbReference type="EnsemblBacteria" id="ABA03964">
        <property type="protein sequence ID" value="ABA03964"/>
        <property type="gene ID" value="Nwi_0698"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0698"/>
    <dbReference type="eggNOG" id="COG0001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_491502_0_0_5"/>
    <dbReference type="OMA" id="RLARYHT"/>
    <dbReference type="OrthoDB" id="493793at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="554" mass="62336" checksum="CB4B440F5271CD69" modified="2005-10-11" version="1">MDLPFPILALSAAAIGGAAAALPKLNARLALSRAKHRSLTGHSRLSRTVARLLPFYEFDHDGFFGSDGAPPEVVKQRSDAFFRLARLYEERYAKGRRMTAEVSSRISDLQFTETYRVPFQYSRMVREHLSTGAFMRSSCGVTVTDVDGNVSYDLAGSYGVNIFGNDFYKDCITAAEKRAHDLGPVLGPYHPVIANNVRRLCEISGLDEVSFHMSGTEAVMQAVRLARYHTRRTHLVRFAGSYHGWWGDVQPGVGNPMPPHETYTLADMSERTLHVLKTRRDIACVLVNPLQALHPNASAPADSALVDSSRKSRFDRAAYSEWLRRLREICTERSIVLIFDEVFVGFRLAAGGAQEYFGVRADMVTYGKSLAGGLPVGAVCGRKDLMRRFRDDRPADVCFARGTFNSHPYVMAAMDEFLSRLAAPGFQAIYDGLDETWNGRAKQLNDLLSARDLPVQVGNLSSIWTVHYTEPSRYNWMLQYYLRAEGLALSWIGTGRLIFSLNYTDADFAAVAERFVTAAENMKRDGWWRRDPSLTNKSVRRQILKEMITRRRPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUV6</accession>
    <name>Q3SUV6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Amino acid/amide ABC transporter ATP-binding protein 2, HAAT family</fullName>
            <ecNumber evidence="7">3.6.3.27</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0669</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in beta-(1--&gt;2)glucan export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.27" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313991.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0669"/>
    <dbReference type="EnsemblBacteria" id="ABA03935">
        <property type="protein sequence ID" value="ABA03935"/>
        <property type="gene ID" value="Nwi_0669"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0669"/>
    <dbReference type="eggNOG" id="COG0410">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_5"/>
    <dbReference type="OMA" id="IVEQMAN"/>
    <dbReference type="OrthoDB" id="1475841at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="2"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="33"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="234" mass="25442" checksum="E06B10D55826A347" modified="2005-10-11" version="1">MLSVADLHVNYGRAEILHGVSLDVGDKPVSVVGRNGMGKTTFCYAIMGMIPVRSGSIALDNEPLDRKSAMQVARRGIAIVPQGRRVFPSLSVDEHLKLASRSRKAAWTPERIYETFPRLAERRSNGGAQLSGGEQQMLAIARALLRNPRLIIMDEPSEGLAPVIVDQLIDTLRKLTLDKIGLLLIEQNLRVATSVSDTVAVMLGGKIAATLPAKRLSTDEALQRRYLGVSSALH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUW0</accession>
    <name>Q3SUW0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Amino acid/amide ABC transporter membrane protein 1, HAAT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0665</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313987.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0665"/>
    <dbReference type="EnsemblBacteria" id="ABA03931">
        <property type="protein sequence ID" value="ABA03931"/>
        <property type="gene ID" value="Nwi_0665"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0665"/>
    <dbReference type="eggNOG" id="COG0559">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039929_2_1_5"/>
    <dbReference type="OMA" id="ADDREMV"/>
    <dbReference type="OrthoDB" id="1841075at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="66"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="195"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="231"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="268"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="297" mass="31114" checksum="68FE0F10D03DEB02" modified="2005-10-11" version="1">MSLELFLTVALNGLTVSALYFIVAIGFSLIFGLMRSVNMAHGTLFLVGAYAGVAVFYPLYDDGNAYAWIIALAAGISASAILGVVLQIVFLGWMQGQELRQTLVTIGISIIVADQLLAIFGGLSQQFYAPDILFGSVPVPFVQGGQYSTFRLFQVVVAIGVGFGLWLILQRTKLGIVVRAGVDDRAMLAAQGVNVPLISVVIFAIGAALAGLAGVIGGTAQPFGQEKDIEFLLTSLVVVIVGGMGSLPGTALGALLVGMAEQFGQSLFPAYSVMLTLLIMVAVLAFRPHGLLGRKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUX4</accession>
    <name>Q3SUX4_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MOSC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0651</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313977.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0651"/>
    <dbReference type="EnsemblBacteria" id="ABA03917">
        <property type="protein sequence ID" value="ABA03917"/>
        <property type="gene ID" value="Nwi_0651"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0651"/>
    <dbReference type="eggNOG" id="COG2258">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082566_1_1_5"/>
    <dbReference type="OMA" id="CWKLNDR"/>
    <dbReference type="OrthoDB" id="1643056at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005302">
        <property type="entry name" value="MoCF_Sase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005163">
        <property type="entry name" value="YiiM-like_3-alpha_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03475">
        <property type="entry name" value="3-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03473">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="SSF50800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51340">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MOSC" evidence="1">
        <location>
            <begin position="29"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="233" mass="25899" checksum="82288E65AE5EF768" modified="2005-10-11" version="1">MARFDVLELRIGGVSPLGSSGALSAIDKHQVHHPLPVSYLGLEGDEQADRKHHGGPHKAIHAYSISHFTTWSKELPDRTNRFRPGAFGENLVIDGASETGLCLGDRWRIGSAVIEVSQGRQPCWKLNLRFDVPDMARRVQDTGRTGWYFRVIEPGILAAGDCGSLIARPNPAWSLTRTSHLLYHDRMNRPALAELAGLPGLPDSWRRLALARLASGKIESWSRRVETPEPYEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV58</accession>
    <name>Q3SV58_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hydroxypyruvate reductase</fullName>
            <ecNumber evidence="3">1.1.1.81</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0566</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.81" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313894.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0566"/>
    <dbReference type="EnsemblBacteria" id="ABA03833">
        <property type="protein sequence ID" value="ABA03833"/>
        <property type="gene ID" value="Nwi_0566"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0566"/>
    <dbReference type="eggNOG" id="COG2379">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032279_1_1_5"/>
    <dbReference type="OMA" id="NESCKPG"/>
    <dbReference type="OrthoDB" id="1358712at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008887">
        <property type="term" value="F:glycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016618">
        <property type="term" value="F:hydroxypyruvate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1480.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037035">
        <property type="entry name" value="GK-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038614">
        <property type="entry name" value="GK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007835">
        <property type="entry name" value="MOFRL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025286">
        <property type="entry name" value="MOFRL_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039760">
        <property type="entry name" value="MOFRL_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12227">
        <property type="entry name" value="PTHR12227"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13660">
        <property type="entry name" value="DUF4147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05161">
        <property type="entry name" value="MOFRL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4147" evidence="2">
        <location>
            <begin position="8"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="MOFRL" evidence="1">
        <location>
            <begin position="311"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="428" mass="44267" checksum="4A3456BF6DB11605" modified="2005-10-11" version="1">MTDQRKLLRAIFDATVAAAHPDTILAAHLPPLPHGRVICLAAGKAAAAMAAAVERHYLDAVGLDPVRLAGLAATRYGHSVPTRRIRVIEAGHPVPDEAGLRSADASLTLASAATADDLLLVLLSGGGSANWIAPVNGVSIARKQQVTKALLRSGAPISDINTVRRHLSRIKGGRLARAASRAEIVTLAISDVPRDEPTVIASGPTVPDPTTLADARALLTKYRIVCDDAVSRALDDPANESCKPGDAAFSRARFEIIARPRTSLDVAARLAREAGFEVLDLGANLEGEARDVAAEHAKLALEARAGGRKLAILSGGELTVTVRGNGRGGPNQEYALSLAARLKDASGVSVLAADTDGADGGAGDASDPAGAVIDTSTFRAMRAFQLDPLAYLADNDATAFFAATGDLLLTGPTLTNVNDLRVVLVDAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV89</accession>
    <name>Q3SV89_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="14">ABC transporter, transmembrane region</fullName>
            <ecNumber evidence="14">3.6.3.27</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Nwi_0535</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in beta-(1--&gt;2)glucan export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.27" evidence="14"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313863.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0535"/>
    <dbReference type="EnsemblBacteria" id="ABA03802">
        <property type="protein sequence ID" value="ABA03802"/>
        <property type="gene ID" value="Nwi_0535"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0535"/>
    <dbReference type="eggNOG" id="COG2274">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_3_5"/>
    <dbReference type="OMA" id="IVGFIRH"/>
    <dbReference type="OrthoDB" id="643917at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005074">
        <property type="entry name" value="Peptidase_C39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03412">
        <property type="entry name" value="Peptidase_C39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50990">
        <property type="entry name" value="PEPTIDASE_C39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="3 10 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="278"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="303"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="410"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C39" evidence="13">
        <location>
            <begin position="11"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="12">
        <location>
            <begin position="172"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="486"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="520"/>
            <end position="527"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABA03802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="744" mass="81829" checksum="5604DF41838B17E2" modified="2005-10-11" version="1">MVAPAVAEDADIDPMPPSGLECLVIIARQHGLDLTSTQLTKDNLLAEREVSPGQLVRCAENAGMRSKHVNLDWDGLSHLKKALPAIVGLRNGSFMVLLAVEGDANNTRIALRDPNAAEDALLVIDRLRFEDIWSGDVILAKRNYEISDESQPFSFGLVAALLFRERRIVRDVAIAALVLGFLGLAPIMFWRLLSDKVIFYHAFNTFYVLCLAMLVIIAFEAVFAFLRQVLVHRLTTRLDVKLSTYVFEKVLNLPIDYFEQTAVGLVARDIHEVYRIRSFLMGQLFGTILDSTTLIFFLPVMFFFSPIMTLIVLTISAMIVGWLMLMLPTYRKVSNAVLVAEGAQGAFLVQTLNGIRTVKSLALDARQRHMWDVLVARVAKARLAEGMTGNAIQAVVRPLERLAVSGSYAVGVYLALVNSDPVYIGALFAFLMLSQRVSGPLMQIAQLINQYDEARSAVATVSKLVNQPAEEGRAGHGVRSPIEGKVEFSNVTFRYKGAVSPSLNGISFEATPGATLGVMGKSGSGKTTITRLLQRLHSDYDGLIKIDGIDVREYDIDHLRRNVGIVLQENFLFSGTIRENICAATPDATFDDIVKAARLAGAEEFIDKLPRGYETYVYEGSPNLSGGQRQRLAIARALIVDPPILILDEATSALDADSEAIVNANIARIASGRTLIVISHRLSSLTTADAILVLDRGVVNDIGRHEELLARNDIYSSLWYQQNTHLAPAVRNDKPKLRSPTLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV99</accession>
    <name>Q3SV99_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cell cycle transcriptional regulator CtrA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0525</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313853.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0525"/>
    <dbReference type="EnsemblBacteria" id="ABA03792">
        <property type="protein sequence ID" value="ABA03792"/>
        <property type="gene ID" value="Nwi_0525"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0525"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_1_5"/>
    <dbReference type="OMA" id="QLWGYPP"/>
    <dbReference type="OrthoDB" id="1020672at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="2"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="4">
        <location>
            <begin position="124"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="124"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="233" mass="26140" checksum="B45C6BAA9F3F8940" modified="2005-10-11" version="1">MRVLLIEDDSAVAQSIELMLKSESFNVYTTDLGEEGVDLGKLYDYDIILLDLNLPDMSGYDVLKQLRVSKIKTPILILSGLAGIEDKVKGLGVGADDYMTKPFHKDELVARIHAIVRRSKGHAQSVIQTGDLVVNLDTKTVEVGGQRVHLTGKEYQMLELLSLRKGTTLTKEMFLNHLYGGMDEPELKIIDVFICKLRKKLANASEGRNFIETVWGRGYVLREPHEDEVRIPA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-01-09" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVI5</accession>
    <name>RL21_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplU</name>
        <name type="ordered locus">Nwi_0439</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein binds to 23S rRNA in the presence of protein L20.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Contacts protein L20.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL21 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313770.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVI5"/>
    <dbReference type="SMR" id="Q3SVI5"/>
    <dbReference type="STRING" id="323098.Nwi_0439"/>
    <dbReference type="EnsemblBacteria" id="ABA03706">
        <property type="protein sequence ID" value="ABA03706"/>
        <property type="gene ID" value="Nwi_0439"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0439"/>
    <dbReference type="eggNOG" id="COG0261">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061463_1_2_5"/>
    <dbReference type="OMA" id="HRQEITR"/>
    <dbReference type="OrthoDB" id="1949059at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01363">
        <property type="entry name" value="Ribosomal_L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036164">
        <property type="entry name" value="L21-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028909">
        <property type="entry name" value="L21p-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001787">
        <property type="entry name" value="Ribosomal_L21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21349">
        <property type="entry name" value="PTHR21349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00829">
        <property type="entry name" value="Ribosomal_L21p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141091">
        <property type="entry name" value="SSF141091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00061">
        <property type="entry name" value="L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000270699" description="50S ribosomal protein L21">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="103"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="109"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="146" mass="15671" checksum="B0E2E72721344FC1" modified="2005-10-11" version="1">MFAVIKTGGRQYRVVPDDVLEIGKIAGEVGTIVQLGEVLVLGGDTPVLGAPTVAGASIAAEVLQHKRGPKVIAFKKRRRKHSKRKRGYRDEITVLRVTEILADGKSPTIGPRPKKEKAVEPVEGASDDKPRRAAKKTAAKTAEDAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVK6</accession>
    <name>Q3SVK6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AFG1-like ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0418</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313749.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0418"/>
    <dbReference type="EnsemblBacteria" id="ABA03685">
        <property type="protein sequence ID" value="ABA03685"/>
        <property type="gene ID" value="Nwi_0418"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0418"/>
    <dbReference type="eggNOG" id="COG1485">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008681_0_1_5"/>
    <dbReference type="OMA" id="FDEMQIT"/>
    <dbReference type="OrthoDB" id="607330at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005654">
        <property type="entry name" value="ATPase_AFG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12169">
        <property type="entry name" value="PTHR12169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03969">
        <property type="entry name" value="AFG1_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="394" mass="43641" checksum="87CAB10193FD5232" modified="2005-10-11" version="1">MLSSPPPSLRNHYQSLVTSGAIEADPAQARAVEALAALDERLMAYKPAGKSGFLDRLFQSDEEPPPKGLYIHGDVGRGKTMLMDLFFQESRVAHKRRVHFHEFMTDVHERIFGFRQSIARGETADADVVQLTAASIFEQAWLLCFDEFHVTDIADAMILGRLFARLFELGTVVVATSNIPPGDLYEGGLNRVLFLPFIAQIEERMDVLRLDARTDFRLEKLASVKMWLTPADAEAEAALDRAWMLMTGGAPCKPRDVAIKGRILHVPCSAHGVARFSFADLCGQPLAASDYLRLARDYHTLMIDRIPVMDHADRDAAKRFIALIDALYDNGVKLMASSDADPLSLYRASDGLEANEFKRTSSRLVEMNSESYLAKAHGRGHSAARSSSTGLVET</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVR9</accession>
    <name>Q3SVR9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Non-homologous end joining protein Ku</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ku</name>
        <name evidence="5" type="ordered locus">Nwi_0355</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">With LigD forms a non-homologous end joining (NHEJ) DNA repair enzyme, which repairs dsDNA breaks with reduced fidelity. Binds linear dsDNA with 5'- and 3'- overhangs but not closed circular dsDNA nor ssDNA. Recruits and stimulates the ligase activity of LigD.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer. Interacts with LigD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the prokaryotic Ku family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313687.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0355"/>
    <dbReference type="EnsemblBacteria" id="ABA03622">
        <property type="protein sequence ID" value="ABA03622"/>
        <property type="gene ID" value="Nwi_0355"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0355"/>
    <dbReference type="eggNOG" id="COG1273">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048975_0_0_5"/>
    <dbReference type="OMA" id="IRYRKVC"/>
    <dbReference type="OrthoDB" id="1724780at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00789">
        <property type="entry name" value="KU_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01875">
        <property type="entry name" value="Prokaryotic_Ku"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006164">
        <property type="entry name" value="Ku70/Ku80_beta-barrel_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009187">
        <property type="entry name" value="Prok_Ku"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016194">
        <property type="entry name" value="SPOC-like_C_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41251">
        <property type="entry name" value="PTHR41251"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02735">
        <property type="entry name" value="Ku"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006493">
        <property type="entry name" value="Prok_Ku"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00559">
        <property type="entry name" value="Ku78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100939">
        <property type="entry name" value="SSF100939"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02772">
        <property type="entry name" value="Ku_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="2" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ku" evidence="4">
        <location>
            <begin position="55"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="256"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="265"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="310"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA03622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="327" mass="36745" checksum="933D01453C0CED51" modified="2005-10-11" version="1">MAPRANWKGFLRLSLVTCPVALFPATSESEKVSFNQINRRTGHRIKYQKVDSETGEEVDNDDIVKGYKIDTDTYLEVTKDELDNIALESTRTIDIDRFVPRAEIDDLYIVRPYYLVPDGKVGHDAYAVIRDTIKATGKVALARVVLTNREHVIALQARDNGLVGLLLRYAYEVREASDYFDDIKDVKITRDMLDLAAHIVEQKSGSFEPTAFEDHYEAALLELIEQKKAGHPIAKARARTEGNVINLMDALRQSLKGGNAERKETPAAAAKDKRSNKRSKKRVEGQREMLFPIEGKKAAKESKREPAKRPAKARAARKSPAARRKAG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVT1</accession>
    <name>LEPA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Elongation factor 4</fullName>
            <shortName evidence="1">EF-4</shortName>
            <ecNumber evidence="1">3.6.5.n1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Ribosomal back-translocase LepA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lepA</name>
        <name type="ordered locus">Nwi_0343</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + H2O = GDP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.n1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.n1" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313675.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVT1"/>
    <dbReference type="SMR" id="Q3SVT1"/>
    <dbReference type="STRING" id="323098.Nwi_0343"/>
    <dbReference type="EnsemblBacteria" id="ABA03610">
        <property type="protein sequence ID" value="ABA03610"/>
        <property type="gene ID" value="Nwi_0343"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0343"/>
    <dbReference type="eggNOG" id="COG0481">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009995_3_3_5"/>
    <dbReference type="OMA" id="MVQIAIQ"/>
    <dbReference type="OrthoDB" id="182107at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03709">
        <property type="entry name" value="lepA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00071">
        <property type="entry name" value="LepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006297">
        <property type="entry name" value="EF-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038363">
        <property type="entry name" value="LepA_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013842">
        <property type="entry name" value="LepA_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035654">
        <property type="entry name" value="LepA_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43512">
        <property type="entry name" value="PTHR43512"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06421">
        <property type="entry name" value="LepA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01393">
        <property type="entry name" value="lepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000224778" description="Elongation factor 4">
        <location>
            <begin position="1"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="domain" description="tr-type G">
        <location>
            <begin position="7"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="19"/>
            <end position="24"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="138"/>
            <end position="141"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00071"/>
        </source>
    </evidence>
    <sequence length="603" mass="66801" checksum="9279B5536FFA0BDD" modified="2005-10-11" version="1">MTTSPIANIRNFSIVAHIDHGKSTLADRLIQMTGGLSDREMAGKEQVLDSMDIERERGITIKAQTVRLKYRAKDGKDYVFNLMDTPGHVDFAYEVSRSLAACEGSLLVVDASQGVEAQTLANVYQALDNNHEIVPVLNKVDLPAAEPDKVKQQIEDVIGIDASDAVMISAKTGLGVPDVLEAIVTRLPPPKGDRDATLKALLVDSWYDVYLGVVVLIRVIDGALRKGSRIRMIGTSAAYDVERVGFFTPKMQQVDELGPGEIGFITAAIKEVADTRVGDTITDDRKPVTEMLPGFKPAIPVVFCGLFPVDADDFETLRAAMGKLRLNDASFSFEMETSAALGFGFRCGFLGLLHLEIVQERLSREFDLNLIATAPSVIYRMRLTDGVEIEIHNPIDMPDVVKIAEIQEPWIEATILTPDEYLGSVLKLCQDRRGVQKELTYVGARAMAKYDLPLNEVVFDFYDRLKSVSKGYASFDYHLTDYKPADLVKMQILVNGEPVDALSMLVHRTRAEGRGRAMVEKMKELIPPHMFQIPIQAAIGGKVIARETVRALRKDVTAKCYGGDVSRKRKLLEKQKEGKKKMRQFGKVDIPQEAFIAALKVDN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-05-25" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVW2</accession>
    <name>IF1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translation initiation factor IF-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">infA</name>
        <name type="ordered locus">Nwi_0312</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the essential components for the initiation of protein synthesis. Stabilizes the binding of IF-2 and IF-3 on the 30S subunit to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps modulate mRNA selection, yielding the 30S pre-initiation complex (PIC). Upon addition of the 50S ribosomal subunit IF-1, IF-2 and IF-3 are released leaving the mature 70S translation initiation complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the 30S ribosomal translation pre-initiation complex which assembles on the 30S ribosome in the order IF-2 and IF-3, IF-1 and N-formylmethionyl-tRNA(fMet); mRNA recruitment can occur at any time during PIC assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IF-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313644.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVW2"/>
    <dbReference type="SMR" id="Q3SVW2"/>
    <dbReference type="STRING" id="323098.Nwi_0312"/>
    <dbReference type="EnsemblBacteria" id="ABA03579">
        <property type="protein sequence ID" value="ABA03579"/>
        <property type="gene ID" value="Nwi_0312"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0312"/>
    <dbReference type="eggNOG" id="COG0361">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151267_4_1_5"/>
    <dbReference type="OMA" id="RCRVKLD"/>
    <dbReference type="OrthoDB" id="2066663at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04451">
        <property type="entry name" value="S1_IF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00075">
        <property type="entry name" value="IF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006196">
        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004368">
        <property type="entry name" value="TIF_IF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33370">
        <property type="entry name" value="PTHR33370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01176">
        <property type="entry name" value="eIF-1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00008">
        <property type="entry name" value="infA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50832">
        <property type="entry name" value="S1_IF1_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000263828" description="Translation initiation factor IF-1">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="S1-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="70"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="93" mass="10618" checksum="6EB9762E99B4E604" modified="2005-10-11" version="1">MAKEELIQFEGLVTEILPDARYRVQLDAGHEIVAYTAGKMKKNRIKTLAGDRVTIEMSPYDLEKGRLIFRHKDERPGGPPRSGPPRGGQFRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVX3</accession>
    <name>Q3SVX3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Type II and III secretion system protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0301</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial secretin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0301"/>
    <dbReference type="EnsemblBacteria" id="ABA03568">
        <property type="protein sequence ID" value="ABA03568"/>
        <property type="gene ID" value="Nwi_0301"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0301"/>
    <dbReference type="eggNOG" id="COG4964">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017952_2_0_5"/>
    <dbReference type="OMA" id="TELMIMV"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007055">
        <property type="entry name" value="BON_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001775">
        <property type="entry name" value="GspD/PilQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032789">
        <property type="entry name" value="T2SS-T3SS_pil_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004846">
        <property type="entry name" value="T2SS/T3SS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004845">
        <property type="entry name" value="T2SS_GspD_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04972">
        <property type="entry name" value="BON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00263">
        <property type="entry name" value="Secretin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13629">
        <property type="entry name" value="T2SS-T3SS_pil_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00811">
        <property type="entry name" value="BCTERIALGSPD"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50914">
        <property type="entry name" value="BON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00875">
        <property type="entry name" value="T2SP_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229346" evidence="2">
        <location>
            <begin position="22"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="BON" evidence="3">
        <location>
            <begin position="119"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="493" mass="51876" checksum="812052CD0EC73469" modified="2005-10-11" version="1">MEWTTKPTSVRAFVARSLAFAAIGAFTLDAALTPVFASDYRVSGAATAGANMHAQSLSLGIGKSVVVDLPRDVKDVLVADPKIANAVVRSAQRAYIIGAAVGQTNIIFFDSSGQQISAYDIAVTRDLNGLRTALKQLLPNAAIKVDGLGDGVMLSGWVSNPIEAQQAGDLAARLTDGAQKVVNNIAVRGRDQVMLKVTVAEVARTVIKQMGIDLSASMNYGTAVVNFTNANPFTALGRPLTNNSAAASFGSTMVGGAPVPAVTATLRAMESAGVIRTLAEPNLTAISGESATFISGGEFPIPAGFSCDPATRVCLTQIRFKKFGISLNFTPVVLTEGRISLRVMTEVSELSNENSMTLNQSVGNSLTVPSIRTRRAETTLEIPSGGSMAMAGLIHQQTKQVINGLPGIAQVPVLGALFRSRDYVNGQTELMVLVTPYVVRAVAQKDLSRPDDGFADASDPQSDLLGSLNRIYGAPGRVEPARHYRGTYGFITD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVX7</accession>
    <name>Q3SVX7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Type II secretion system protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_0297</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313630.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0297"/>
    <dbReference type="EnsemblBacteria" id="ABA03564">
        <property type="protein sequence ID" value="ABA03564"/>
        <property type="gene ID" value="Nwi_0297"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0297"/>
    <dbReference type="eggNOG" id="COG4965">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064305_1_1_5"/>
    <dbReference type="OMA" id="FLNWQRQ"/>
    <dbReference type="OrthoDB" id="1002350at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.81.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042094">
        <property type="entry name" value="T2SS_GspF_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="265"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="297"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="T2SSF" evidence="7">
        <location>
            <begin position="158"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA03564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="324" mass="35243" checksum="8ED642901BCDEE1B" modified="2005-10-11" version="1">MNLQTLALAFMAATAVGGVAWVFLYPVLSGEMKAEKRRAALARTSPAIRQVDRAQRSRREQIEESLREIEQRQKRDRKVTLNNRIAQAGLSWSKEKFLIVSGILALASFAVPIVLGAGPLAAAGLAFAAGFGVPRWLLGFLKKRRETAFLRALPDAVDVIVRGIKAGLPLFESLKVVAADSPEPLKSEFAAIIETQAIGMPLGEACSRLYERMPVPEANFFGIVIAIQQKSGGNLSEALGNLSKVLRDRKKMAEKIKAVSMEAKASAAIIGSLPPIVMLLVYLSTPDYIALLWTHPTGRLMLAGSALWMTAGILVMKKMINFDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW15</accession>
    <name>Q3SW15_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4365 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0259</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0259"/>
    <dbReference type="EnsemblBacteria" id="ABA03526">
        <property type="protein sequence ID" value="ABA03526"/>
        <property type="gene ID" value="Nwi_0259"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0259"/>
    <dbReference type="eggNOG" id="COG5474">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_949391_0_0_5"/>
    <dbReference type="OMA" id="GLTNICR"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025375">
        <property type="entry name" value="DUF4365"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14280">
        <property type="entry name" value="DUF4365"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4365" evidence="1">
        <location>
            <begin position="13"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="293" mass="33024" checksum="5F3B5FFA942824A5" modified="2005-10-11" version="1">MDLPKVGNTYAQERAGIAAVQGYAADRKQIWRETGTGDVGIDGQLEFVNEQALATGRTVAVQVKAGPSYFQHETAAGWKFYPEAKHRNYWEAFPLPVLLVLHNTETSLSYWTDARQALRTPTREERAYIEIPGTNVLQKSDPMTLFENAGVQDQPFIPDLHDVLKRLLATESREGTFPLSHFDLFVHGLTNICRSIYYGMDAVCNAVEYNLSVRKSEFGMGMGEPEHNFVFGFVKLLVAQNLAQIDYADCLIDWIDRQMQPHFVAPLTSRGRALVALIHEEERAIGRCRCSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW18</accession>
    <name>Q3SW18_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0256</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313589.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0256"/>
    <dbReference type="EnsemblBacteria" id="ABA03523">
        <property type="protein sequence ID" value="ABA03523"/>
        <property type="gene ID" value="Nwi_0256"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0256"/>
    <dbReference type="HOGENOM" id="CLU_1617280_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="164" mass="18794" checksum="0744C68B1853FB87" modified="2005-10-11" version="1">MAVTRRLVRRSFSEGGRRGAVHESPVLRRLGGAGRVKVPHLILLLPFPMYEGAETMKEERNRKSRHEPRGKFVTGEDYKKTWGTPLGQDCIDLHETLARWLGERLVFLADHTTTVTPDAAEPWHEALRRNGEALLRYAAEWPEGEGEAKRSLEWVARNLESLWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW39</accession>
    <name>Q3SW39_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0234</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313569.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0234"/>
    <dbReference type="EnsemblBacteria" id="ABA03502">
        <property type="protein sequence ID" value="ABA03502"/>
        <property type="gene ID" value="Nwi_0234"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0234"/>
    <dbReference type="eggNOG" id="ENOG503349C">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1266085_0_0_5"/>
    <dbReference type="OMA" id="KFPTEIP"/>
    <dbReference type="OrthoDB" id="1751330at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="236" mass="25045" checksum="4E12B11C6453B096" modified="2005-10-11" version="1">MLDVADTAEGEPGAAGRFRCFPWGGLFLSRFSSLMTMVAVCCTVAAFPVQSQSLGDRFKGMFGIRSQPEQTAPAPGDTASVPDYLTCPSVAIRSGASTYAVGESGKPATGAEVRYQATIRDTARECDYNSDTQQISVKVGIRGRVIVGPAGAPPTVEVPLRVAVVEDGISPKTIATNAYTITVSLSDSGGGIYSFVSDDLSYPAPQGAAADRYVFYVGFDPQALKPEPRMNRRRQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW51</accession>
    <name>Q3SW51_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Invasion associated locus B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0222</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0222"/>
    <dbReference type="EnsemblBacteria" id="ABA03490">
        <property type="protein sequence ID" value="ABA03490"/>
        <property type="gene ID" value="Nwi_0222"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0222"/>
    <dbReference type="eggNOG" id="COG5342">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096085_0_1_5"/>
    <dbReference type="OMA" id="HGDWQIR"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038696">
        <property type="entry name" value="IalB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010642">
        <property type="entry name" value="Invasion_prot_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06776">
        <property type="entry name" value="IalB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="195" mass="20512" checksum="61F038BD6D9018A9" modified="2005-10-11" version="1">MVESRGSTRMEASKQHIGYASSSRRTARRQPLRILACGAAALLVAGFASAAKAQGAVKSVHGDWQIRCDTPPGAKAEQCALIQSVVAEDRSNAGLTVIVLKTADQKSRLMRVVAPLGVLLPSGLGLKLDNADVGRAGFVRCLPNGCVAEVVLDDKLLGQLRSAKTATFIIFETPEEGIGFPLSLNGLGEGYDKLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW70</accession>
    <name>Q3SW70_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DNA-O6-methylguanine--protein-cysteine S-methyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.63</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_0203</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 4-O-methyl-thymidine in DNA + L-cysteinyl-[protein] = a thymidine in DNA + S-methyl-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:53428"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:13555"/>
            <dbReference type="Rhea" id="RHEA-COMP:13556"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:137386"/>
            <dbReference type="ChEBI" id="CHEBI:137387"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 6-O-methyl-2'-deoxyguanosine in DNA + L-cysteinyl-[protein] = a 2'-deoxyguanosine in DNA + S-methyl-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:24000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:11367"/>
            <dbReference type="Rhea" id="RHEA-COMP:11368"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:85445"/>
            <dbReference type="ChEBI" id="CHEBI:85448"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.63" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0203"/>
    <dbReference type="EnsemblBacteria" id="ABA03471">
        <property type="protein sequence ID" value="ABA03471"/>
        <property type="gene ID" value="Nwi_0203"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0203"/>
    <dbReference type="eggNOG" id="COG0350">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2169">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_52_0_5"/>
    <dbReference type="OMA" id="ALTGYHW"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003908">
        <property type="term" value="F:methylated-DNA-[protein]-cysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06445">
        <property type="entry name" value="ATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001497">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014048">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036217">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNAb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036631">
        <property type="entry name" value="MGMT_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01035">
        <property type="entry name" value="DNA_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46767">
        <property type="entry name" value="SSF46767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53155">
        <property type="entry name" value="SSF53155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00589">
        <property type="entry name" value="ogt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00374">
        <property type="entry name" value="MGMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="6">
        <location>
            <begin position="44"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="314" mass="33929" checksum="C9F674CE36297F62" modified="2005-10-11" version="1">MAGRRRTSYILAMMTLAKIPAPADSRMTQPDAPGAALRDYDAVRRAIAFISENWRSQPAIAATADAAGVTPDELHHLFRRWAGLTPKLFMQALTLDHAKRLLRKSASVLDAALDSGLSGPGRLHDLFVTHEAMSPGEWKNGGSGMKLAFGFHPSPFGIAIVIASDRGLAGLAFADGGDEQAALADMKRRWPNAAYVEDAARTGALAQRVFDTRLWRADQPLRVVLIGTDFEVRVWQTLLRIPMGKVTTYSTIAASIDRPTASRAVGAAVGKNPVSFVVPCHRVLGKSGALTGYHWGITRKHAMLGWEAGRIGLE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW71</accession>
    <name>GPMA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">2,3-bisphosphoglycerate-dependent phosphoglycerate mutase</fullName>
            <shortName evidence="1">BPG-dependent PGAM</shortName>
            <shortName evidence="1">PGAM</shortName>
            <shortName evidence="1">Phosphoglyceromutase</shortName>
            <shortName evidence="1">dPGM</shortName>
            <ecNumber evidence="1">5.4.2.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gpmA</name>
        <name type="ordered locus">Nwi_0202</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate</text>
            <dbReference type="Rhea" id="RHEA:15901"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="5.4.2.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 3/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.11" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313538.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SW71"/>
    <dbReference type="SMR" id="Q3SW71"/>
    <dbReference type="STRING" id="323098.Nwi_0202"/>
    <dbReference type="EnsemblBacteria" id="ABA03470">
        <property type="protein sequence ID" value="ABA03470"/>
        <property type="gene ID" value="Nwi_0202"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0202"/>
    <dbReference type="eggNOG" id="COG0588">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033323_1_4_5"/>
    <dbReference type="OMA" id="RMLPYWY"/>
    <dbReference type="OrthoDB" id="1122642at2"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00186"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046538">
        <property type="term" value="F:2,3-bisphosphoglycerate-dependent phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01039">
        <property type="entry name" value="PGAM_GpmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005952">
        <property type="entry name" value="Phosphogly_mut1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11931">
        <property type="entry name" value="PTHR11931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="SSF53254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01258">
        <property type="entry name" value="pgm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0312">Gluconeogenesis</keyword>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000229129" description="2,3-bisphosphoglycerate-dependent phosphoglycerate mutase">
        <location>
            <begin position="1"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="1">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="23"/>
            <end position="24"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="89"/>
            <end position="92"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="116"/>
            <end position="117"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="160"/>
            <end position="161"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01039"/>
        </source>
    </evidence>
    <sequence length="207" mass="22976" checksum="3B39AC6EB6BDC2C0" modified="2005-10-11" version="1">MSERLLVLVRHGQSEWNLKNLFTGWKDPDLTELGAAEAKDAGRKLKAQGFVFDIAFTSTLIRAQHTLDLVLKELDQTGIPVRKDQALNERDYGDLSGLNKDEARKKWGDEQVLVWRRSYDVPPPGGESLKDTLARTLPYFVQEILPCVLRGECTLVAAHGNSLRALVMVLEKLSPEQILARELATGAPVIYRLNADATVASKLDLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="134" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW75</accession>
    <name>Q3SW75_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Chaperone protein DnaJ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">dnaJ</name>
        <name evidence="12" type="ordered locus">Nwi_0198</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 2 Zn(2+) ions per monomer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DnaJ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313534.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0198"/>
    <dbReference type="EnsemblBacteria" id="ABA03466">
        <property type="protein sequence ID" value="ABA03466"/>
        <property type="gene ID" value="Nwi_0198"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0198"/>
    <dbReference type="eggNOG" id="COG0484">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017633_0_7_5"/>
    <dbReference type="OMA" id="DLHCTVT"/>
    <dbReference type="OrthoDB" id="1738789at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01152">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012724">
        <property type="entry name" value="DnaJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="SSF46565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="SSF49493"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="SSF57938"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02349">
        <property type="entry name" value="DnaJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 8" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 8 12" id="KW-0346">Stress response</keyword>
    <keyword evidence="5 8 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="10">
        <location>
            <begin position="6"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="11">
        <location>
            <begin position="135"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif" evidence="8">
        <location>
            <begin position="148"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif" evidence="8">
        <location>
            <begin position="165"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif" evidence="8">
        <location>
            <begin position="187"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif" evidence="8">
        <location>
            <begin position="201"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="9">
        <location>
            <begin position="135"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA03466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="378" mass="40958" checksum="1D94BD72C81D07A5" modified="2005-10-11" version="1">MSTKRCYYETLEVDRNADDTKLKAAFRKLAMKWHPDRNPGDSSSEMRFKEINEAYEVLKDSNKRAAYDRFGHAAFEQGGMGGGAAGFGAGFASSFSDIFEDLFGMSGQRGRSGGRERGGDLRYNMEITLEEAYHGKTAQIEIPVSVTCESCSGTGAKAGTKPKNCAMCGGAGRIRQAQGFFTLERTCPGCQGRGQMIEDPCPSCGGSGRVNRERSLSVNIPAGVEDGTRIRLAGEGEAGVRGGPPGDLYIFLSLIPHQFFQRDGADLHCRVPISMVAAALGGEIEVPTIDKGKTKVRVPSGTQSGRRFRIATKGMPVLRSRQTGDMYVQVVVETPQNLTKRQQDLLAEFEKLSSGETQPEAAGFFTKVKDFFGTRATP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWH6</accession>
    <name>MNMG_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glucose-inhibited division protein A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mnmG</name>
        <name evidence="1" type="synonym">gidA</name>
        <name type="ordered locus">Nwi_0097</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Heterotetramer of two MnmE and two MnmG subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MnmG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313434.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWH6"/>
    <dbReference type="SMR" id="Q3SWH6"/>
    <dbReference type="STRING" id="323098.Nwi_0097"/>
    <dbReference type="EnsemblBacteria" id="ABA03365">
        <property type="protein sequence ID" value="ABA03365"/>
        <property type="gene ID" value="Nwi_0097"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0097"/>
    <dbReference type="eggNOG" id="COG0445">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007831_2_2_5"/>
    <dbReference type="OMA" id="FRPGYAI"/>
    <dbReference type="OrthoDB" id="146811at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00129">
        <property type="entry name" value="MnmG_GidA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004416">
        <property type="entry name" value="MnmG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002218">
        <property type="entry name" value="MnmG-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020595">
        <property type="entry name" value="MnmG-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026904">
        <property type="entry name" value="MnmG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044920">
        <property type="entry name" value="MnmG_C_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040131">
        <property type="entry name" value="MnmG_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806">
        <property type="entry name" value="PTHR11806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806:SF0">
        <property type="entry name" value="PTHR11806:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01134">
        <property type="entry name" value="GIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13932">
        <property type="entry name" value="GIDA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00136">
        <property type="entry name" value="gidA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01280">
        <property type="entry name" value="GIDA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01281">
        <property type="entry name" value="GIDA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_1000016629" description="tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG">
        <location>
            <begin position="1"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="11"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="270"/>
            <end position="284"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00129"/>
        </source>
    </evidence>
    <sequence length="623" mass="66650" checksum="ECD4A6E6FA676D74" modified="2005-10-11" version="1">MTASFDVIVIGGGHAGCEAAAASARTGARTALVTHRFATIGAMSCNPAIGGLGKGHLVREVDALDGLMARIADAGGIQFRMLNRRKGPAVRGPRAQEDRKLYAAAMQAAIRETANLEVIDGEADDLIVSGGRIAGVRLTDGRRIKSAAVVVTTGTFLRGLIHLGEKSWPAGRVDEAPALGLSASFERIGFALGRLKTGTPPRLDGTTIDWAAVEMQPGDDPPEPFSVLTARITTPQIQCGITRTTPATHDLIRTNVHRSPIYSGQITSSGPRYCPSIEDKVVRFGDRDGHQVFLEPEGLDDATVYPNGISTSLPEEVQLAILATIPGLENVKMTRPGYAIEYDHVDPRELEPTLQTRRMRGLFLAGQINGTTGYEEAAAQGLVAGLNAALAAGGGDPVVFDRADGYLGVMIDDLVTRGITEPYRMFTSRAEYRLTLRADNADQRLTDRGIALGCVGAGRAAYHRAKMAALQAAKQGAMSLSVTPNEAARHGLSLNRDGHRRSAFELLSYPEIGWREVAAIWPELSAIDPAIAEHLEIDAKYDVYLKRQAADVEAFRRDEGLVLTEIDYSAVPGLSNEARNRLEKAQPLTVGQAGRLDGITPAALGILAAYLRREARQRTAASA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-06-12" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWJ8</accession>
    <name>Y075_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0434 protein Nwi_0075</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">Nwi_0075</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0434 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313412.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWJ8"/>
    <dbReference type="SMR" id="Q3SWJ8"/>
    <dbReference type="STRING" id="323098.Nwi_0075"/>
    <dbReference type="EnsemblBacteria" id="ABA03343">
        <property type="protein sequence ID" value="ABA03343"/>
        <property type="gene ID" value="Nwi_0075"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0075"/>
    <dbReference type="eggNOG" id="COG2835">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155659_2_0_5"/>
    <dbReference type="OMA" id="ELICHAD"/>
    <dbReference type="OrthoDB" id="1834382at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01187">
        <property type="entry name" value="UPF0434"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005651">
        <property type="entry name" value="Trm112-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03966">
        <property type="entry name" value="Trm112p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000291120" description="UPF0434 protein Nwi_0075">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01187"/>
        </source>
    </evidence>
    <sequence length="66" mass="7361" checksum="A1DADDEEE587390C" modified="2005-10-11" version="1">MTPPHSDRPEDTADPKLLEILVCPVTKGPLELDGARRELISRSAKLAYPIRDGIPIMLPEEARKIE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWP7</accession>
    <name>TRUB_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA pseudouridine synthase B</fullName>
            <ecNumber evidence="1">5.4.99.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridine(55) synthase</fullName>
            <shortName evidence="1">Psi55 synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridylate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA-uridine isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">truB</name>
        <name type="ordered locus">Nwi_0026</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>uridine(55) in tRNA = pseudouridine(55) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42532"/>
            <dbReference type="Rhea" id="RHEA-COMP:10101"/>
            <dbReference type="Rhea" id="RHEA-COMP:10102"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase TruB family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313365.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWP7"/>
    <dbReference type="SMR" id="Q3SWP7"/>
    <dbReference type="STRING" id="323098.Nwi_0026"/>
    <dbReference type="PRIDE" id="Q3SWP7"/>
    <dbReference type="EnsemblBacteria" id="ABA03294">
        <property type="protein sequence ID" value="ABA03294"/>
        <property type="gene ID" value="Nwi_0026"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0026"/>
    <dbReference type="eggNOG" id="COG0130">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032087_0_3_5"/>
    <dbReference type="OMA" id="ELQFIRW"/>
    <dbReference type="OrthoDB" id="1166299at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106029">
        <property type="term" value="F:tRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02573">
        <property type="entry name" value="PseudoU_synth_EcTruB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01080">
        <property type="entry name" value="TruB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014780">
        <property type="entry name" value="tRNA_psdUridine_synth_TruB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015240">
        <property type="entry name" value="tRNA_sdUridine_synth_fam1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032819">
        <property type="entry name" value="TruB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13767">
        <property type="entry name" value="PTHR13767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09157">
        <property type="entry name" value="TruB-C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16198">
        <property type="entry name" value="TruB_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00431">
        <property type="entry name" value="TruB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000229363" description="tRNA pseudouridine synthase B">
        <location>
            <begin position="1"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="358" mass="38377" checksum="30EA9AEB7DE6CA85" modified="2005-10-11" version="1">MTTPDAAIDSKISDSNGADKNKSAADDNAFNAPGRKRHHNNQPRRDKRDVHGWVALDKPVGMTSTQAVAVVKRLFSAKRAGHAGTLDPLASGGLPIALGEATKTVPFVMDGRKRYRFTVAWGEERDTDDTEGRAVATSAERPTAEAIMALLPSFTGKIEQIPPQYSAVKIQGERAYDLARDGEVVPLAPRPVEIHHLNLADHQDSGHSVFEAECGKGTYVRALARDMGRLLGCYGHICALRRTLVGPFDETSMIPLEHLQALCDRAASGEGNLADALLPVETALDDIPALAVTRADAARLHRGQAVLLRGRDAPNMSGTVYVTVAGRLLALAELGNGELIPKRVFNLSGLTASPHRQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="133" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMU7</accession>
    <name>Q3SMU7_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">DNA gyrase subunit B</fullName>
            <ecNumber evidence="4 12">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">gyrB</name>
        <name evidence="14" type="ordered locus">Nwi_0004</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="12">Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the type II topoisomerase GyrB family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the type II topoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="4 12"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313343.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0004"/>
    <dbReference type="EnsemblBacteria" id="ABA03272">
        <property type="protein sequence ID" value="ABA03272"/>
        <property type="gene ID" value="Nwi_0004"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0004"/>
    <dbReference type="eggNOG" id="COG0187">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006146_4_1_5"/>
    <dbReference type="OMA" id="LWETTMH"/>
    <dbReference type="OrthoDB" id="205481at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03366">
        <property type="entry name" value="TOPRIM_TopoIIA_GyrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.670">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01898">
        <property type="entry name" value="GyrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002288">
        <property type="entry name" value="DNA_gyrase_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011557">
        <property type="entry name" value="GyrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001241">
        <property type="entry name" value="Topo_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013759">
        <property type="entry name" value="Topo_IIA_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013506">
        <property type="entry name" value="Topo_IIA_bsu_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018522">
        <property type="entry name" value="TopoIIA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034160">
        <property type="entry name" value="TOPRIM_GyrB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00204">
        <property type="entry name" value="DNA_gyraseB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00986">
        <property type="entry name" value="DNA_gyraseB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00433">
        <property type="entry name" value="TOP2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="SSF56719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01059">
        <property type="entry name" value="gyrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00177">
        <property type="entry name" value="TOPOISOMERASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 12 14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 12" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Toprim" evidence="13">
        <location>
            <begin position="433"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABA03272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="813" mass="89698" checksum="E2AC610DE795315D" modified="2005-10-11" version="1">MNEPARQQIADTESVAAIEYGAESIRVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALAGHATAVEVILNADNSVTVRDDGRGIPVDIHKGEGVSAAEVIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSSKLTLRIWRRDKEHLIEFANGDALAPLKVVGDANGRRGTEVTFLASTETFTNIEYDFATLEHRLRELAFLNSGVNILLSDMRHAVERREQMMYEGGVVEFVKYLDRNKKAMVADPIMVRAEMNGISVEAALWWNDSYHENVLCFTNNIPQRDGGTHLAGFRGALTRQVNGYADAMAKKERIALTGDDCREGLTAVLSVKVPDPKFSSQTKDKLVSSEVRPVVENVINEALAAWFEEHPTEAKLIVGKVVEAAAAREAARKARELTRRKGALDVASLPGKLADCQERDPAKSELFIVEGDSAGGSAKQGRNREFQAVLPLRGKILNVERARFDKMLSSEQIGTLITALGTGIGREDFDLSKLRYHKIIVMTDADVDGAHIRTLLLTFFFRQMPALIEAGHLYIAQPPLYKVTRGKSERYLKDERALEDYLIETGLDDCVYKLATGEDRGGRDLLALVDDARVIRNVLQNLHGRYNRGVIEQAAIAGVMNPRITENLATATAAADYIARRLDALADEVERGWVGRFVEGQGFFFERTVRGVKDVAMIDDALLGSADARKLDEYAGRLQEAYPRPGLLRRRDTETAIHGPVSLFEAVTDAGRKGVALQRYKGLGEMNPDQLWETTLDVSERSLLQVKIKEVDEADDIFTKLMGDVVEPRREFIQDNSLSANVDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="134" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMW0</accession>
    <name>Q3SMW0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Heavy metal translocating P-type ATPase</fullName>
            <ecNumber evidence="10">3.6.3.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_3134</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.3" evidence="10"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316289.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3134"/>
    <dbReference type="EnsemblBacteria" id="ABA06381">
        <property type="protein sequence ID" value="ABA06381"/>
        <property type="gene ID" value="Nwi_3134"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3134"/>
    <dbReference type="eggNOG" id="COG2217">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001771_6_4_5"/>
    <dbReference type="OMA" id="FAQPWET"/>
    <dbReference type="OrthoDB" id="237367at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00941">
        <property type="entry name" value="CDATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="SSF55008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="SSF81653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="102"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="325"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="357"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="659"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="9">
        <location>
            <begin position="6"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="70"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="77"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA06381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="712" mass="73791" checksum="739D358BAD6BAB1C" modified="2005-10-11" version="1">MATASPLKLRIEGMDCGACAAKIENAMQRLPGVSDINVNYGLQSLSLAVDEDRTSRTTIEAKIRALGYTPADPSEHVMSSSRRDDDRDSAEGTWWSSSKGRLVIGTGALLLLAFAVSYIEPDWSNWAYIAATLVGLIPIGRRALAGALSGTPFSIETLMSIAAIGAVAIGEAGEAAVVVFLFAVGELLEGVAAGRARAGIKALIDLVPRTARRQTGSDIETVPVGDLVVGDAVIVRPGDRVPSDGTVIEGVSEVNEAPVTGESVPVGKEPGSTVYAGSINANGELRVKITRTAADNTIARIVHMVEEAQGSKAPTARFIDRFSRWYTPAAMAVSALVVLVPPLMFGADWFTWVYRGLAVLLIACPCALVISTPAAIASGLASGARRGLLIKGGAALETLGKVRTVAFDKTGTLTMGHPQVTDIVVIEGTEADILAKSAAVERGSSHPLGAAIVAEAERRGLEIPKAFGGGIATPGKAVTARLKAGFVSVGSPRHASEQGEVPEKVRKQIEALESQGKTVVVVSEGKQLVGLIALRDEPRPDAADGLAKLRALGIRPVMLTGDNARTAAAIGDALGLEIRAELLPDAKLDAISVYKSEGPIAMVGDGINDAPALAASSVGIAMGGGTDVALETADAALLKNRVTGVAELIALSRATLANIWQNIMLALGLKSVFLVTTLFGATPLWMAILADTGATVLVTANALRLLRLEFKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SMZ0</accession>
    <name>Q3SMZ0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_3104</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06351">
        <property type="protein sequence ID" value="ABA06351"/>
        <property type="gene ID" value="Nwi_3104"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3104"/>
    <dbReference type="HOGENOM" id="CLU_2539147_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="83" mass="9743" checksum="9E3E21F9F9BD468C" modified="2005-10-11" version="1">MRTYTRRSGTDFHNVLTAKQPTRRTCRGLSDKQKADAVRAYGPHAIWKDVGQRLLDNTCPHRTGRHEVNGYWPGWERLLQRRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN34</accession>
    <name>Q3SN34_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Polyhydroxyalkanoate synthesis repressor PhaR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_3059</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316223.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3059"/>
    <dbReference type="EnsemblBacteria" id="ABA06307">
        <property type="protein sequence ID" value="ABA06307"/>
        <property type="gene ID" value="Nwi_3059"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3059"/>
    <dbReference type="eggNOG" id="COG5394">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089210_0_0_5"/>
    <dbReference type="OMA" id="QEKFRKQ"/>
    <dbReference type="OrthoDB" id="1665699at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012909">
        <property type="entry name" value="PHA_DNA-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010134">
        <property type="entry name" value="PHA_reg_PhaR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007897">
        <property type="entry name" value="PHB_accumulat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05233">
        <property type="entry name" value="PHB_acc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07879">
        <property type="entry name" value="PHB_acc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01848">
        <property type="entry name" value="PHA_reg_PhaR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PHB_acc_N" evidence="3">
        <location>
            <begin position="9"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="PHB_acc" evidence="2">
        <location>
            <begin position="74"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA06307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="197" mass="22850" checksum="6FCFF56181B3AD3C" modified="2005-10-11" version="1">MVKSETPTTIKKYANRRLYNTGTSTYVTLEDLAAMVKQGEDFLVFDAKTGDDITRSVLAQIIFEQENKTGQNLLPTTFLRQLIRFYGDSMQMVVPKYLERSIDLLTREQEKFRKQLTSSFSGTPFAPLEEHVRRNMELFERTFSMFKPFVPPRGGAAPEPEKTPDAPDPGDNIEDLRRQMKEMQDRLERMSKEPKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN46</accession>
    <name>Q3SN46_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fec I like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_3045</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3045"/>
    <dbReference type="EnsemblBacteria" id="ABA06295">
        <property type="protein sequence ID" value="ABA06295"/>
        <property type="gene ID" value="Nwi_3045"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3045"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_12_3_5"/>
    <dbReference type="OMA" id="MAWLRDI"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="2">
        <location>
            <begin position="23"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="3">
        <location>
            <begin position="122"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA06295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="182" mass="20933" checksum="491ECCADF6FE77ED" modified="2005-10-11" version="1">MIARNLRDRGDKARPVTFDVHDLFRRHSKELTRFLRRRVPSADVAADLTQEAFLALWAARSRTSIADGHAYLFRTATNLAINHNRRQRILSFVDDPDTALATLADETPSAEQVLLSRQELMIVYATLKEFPPAHRAVFILSRIDGRTFAEIGRMLDIQPRTAFGHMVRILARMKLRLDEARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN48</accession>
    <name>Q3SN48_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">TonB-dependent siderophore receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Nwi_3043</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the TonB-dependent receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316209.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3043"/>
    <dbReference type="EnsemblBacteria" id="ABA06293">
        <property type="protein sequence ID" value="ABA06293"/>
        <property type="gene ID" value="Nwi_3043"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3043"/>
    <dbReference type="eggNOG" id="COG4773">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008287_9_0_5"/>
    <dbReference type="OMA" id="VETEYHY"/>
    <dbReference type="OrthoDB" id="173242at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015344">
        <property type="term" value="F:siderophore uptake transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012910">
        <property type="entry name" value="Plug_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037066">
        <property type="entry name" value="Plug_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011662">
        <property type="entry name" value="Secretin/TonB_short_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039423">
        <property type="entry name" value="TonB-dep_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000531">
        <property type="entry name" value="TonB-dep_rcpt_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036942">
        <property type="entry name" value="TonB_rcpt_b-brl_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010105">
        <property type="entry name" value="TonB_sidphr_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32552">
        <property type="entry name" value="PTHR32552"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07715">
        <property type="entry name" value="Plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00593">
        <property type="entry name" value="TonB_dep_Rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00965">
        <property type="entry name" value="STN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01783">
        <property type="entry name" value="TonB-siderophor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="4 8" id="KW-0798">TonB box</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="STN" evidence="9">
        <location>
            <begin position="82"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABA06293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="819" mass="88268" checksum="3EA935D92F42BC1F" modified="2005-10-11" version="1">MRSGAERRLVTEAWADASNGCAIVLLLSTVLTTGMVGVANAQTPGSSAPVAAPAARSASYSFSIPPQPLAGAISAFSRVTGIDVVGDGGIGRGVGSPGVAGNLTPHQALTRLLAGTGLSYRFTNASTVAIQRPNAGGGIGAAPSGSISLDTIDVQGETAWGPVNGFVASRSATATKTDTPIIEVPQSISVVTQDQMQTRQAQSVNETLNYTPGVLAGSNGNQFNSTGAQIRGFNASPTSGSLYLNGLRSINVGDIDPYALERVEVMRGPASVLYGQNEPGGVIGLVTKRPTERPFHEVRLQGGSFGQRGGGFDFGGPVTEDRSLLYRFTGLMREGGNGIDFSNDRRALLSGAVTWRPTDVTELNAFAMHQRDDSKWNYGLPARGTALPNPNGRIPITRYIGEPAFDYSNTETTAIGYNFQHRATDSLVFRQNLQYSHEIWDYRDVAPVGLQADLRTLDRSASRVRYGWDTFAVDNHAELKATTGVLHHTVLFGVDYRMRKSTQQGTWDGVVGPIDVFAPVYGSPVFMPADNFGSVQTQNLTGIYLQDQIKLERWILTLGGRHDWVDTRTVGIFNNAVNNQNSSAFTKRAGLGYEFDSGMVPYISYSESFVPTSGTTFDGTPFKPTTGIQHEAGIKFQPKDTNLRMTAAVYDLRRQNVTTIDPAHPGFSIQIGEVTSRGFEFEAVASLTSNLNLTAAYAYNDARVTRSNGADLGKRPFRSPPHLASLWADYTIREGALNGLGFGGGIHYVSASVGDALNTFYAPAYTVVDAMIRYDIDNWRFSVNATNLFDTQYVAACFFIDQCNVGRVRTVLGRVSYRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN51</accession>
    <name>Q3SN51_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Adenylyl cyclase class-3/4/guanylyl cyclase</fullName>
            <ecNumber evidence="4">4.6.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_3040</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.6.1.1" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316206.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3040"/>
    <dbReference type="EnsemblBacteria" id="ABA06290">
        <property type="protein sequence ID" value="ABA06290"/>
        <property type="gene ID" value="Nwi_3040"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3040"/>
    <dbReference type="eggNOG" id="COG2114">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3850">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018096_0_0_5"/>
    <dbReference type="OMA" id="VFCDLRG"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009190">
        <property type="term" value="P:cyclic nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13185">
        <property type="entry name" value="GAF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="280"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="3">
        <location>
            <begin position="304"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="2">
        <location>
            <begin position="620"/>
            <end position="751"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA06290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="815" mass="87961" checksum="2E3414096DA9A6EF" modified="2005-10-11" version="1">MNEAENNSRSPRRGLFVKYVMSLVGLVLFVLAVNGALEIWISYNQTKTALVQAMSEKADAAAHRISQAMVDLERQVSWVTRASVVTVEQRRADYAELLNQVPAVDRIFQLDGSGREQLRLSRRSVLVGSGADFSRDARFTRTIAQGVTYAPVFFRDRRPFMSIAVAHAGRDAGASVAEVDLRFLSGYVDDAQGGKAGSAYVVDASGILLASSDKTTHPGENLSSRPQVASLLNAGHGSSASGTNNQGQAVLTASSPVSTLRWFVVLEQPASLAFAPILDLLIRIGILVAFGLVVAIIAGMLLARRMLVPIQALSGGARRLGAGDFGHRIDIKTGDELEQLAERFNSMAGQLQETYSDLELKVKERTRDLAQSVNELKVLEEVGRAVVSSLDLEAVLSTVAARALEITKADAVLIYGFDAGSRRFDLVQASGIEKSAPGDHLVIGSDDSLLGAAAANGQSIAIEDLASAEANPLNEVAASAGFRSVLVVPLVDQQGVLGSLIMLRRSAGAFSPNLVGLMQTFAHQAASAMRNARLFHEVDRKGKELAIAHGVVQQQADKLQEQTYRLLNWNRSLEERVEAQLGEIERIRRLERFLAPQVAQIVASSDGQNSLLDSHRGEVTVVFCDLRGFTAFTESSEPEEAMNVLREYHAALGELIFKYEGTLDRYAGDGVMVLFNAPVPVPDHTPRAIRMAVEMRDAIGLLSQKWRSRGHSLGFSVGIAVGYATLGQVGFERRLEYAAIGSVTNLASRLCDEAKAGQIVVNQRAYGMVEHLVEAAELAPMVLKGFHHPIVAVEIHRWRDPAEGGSVRSAAGRTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN66</accession>
    <name>Q3SN66_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycoside hydrolase 15-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_3025</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316193.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3025"/>
    <dbReference type="CAZy" id="GH15">
        <property type="family name" value="Glycoside Hydrolase Family 15"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06275">
        <property type="protein sequence ID" value="ABA06275"/>
        <property type="gene ID" value="Nwi_3025"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3025"/>
    <dbReference type="eggNOG" id="COG3387">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010399_3_1_5"/>
    <dbReference type="OMA" id="VEWMCVP"/>
    <dbReference type="OrthoDB" id="318658at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011613">
        <property type="entry name" value="GH15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045582">
        <property type="entry name" value="Trehalase-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19291">
        <property type="entry name" value="DUF5911"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00723">
        <property type="entry name" value="Glyco_hydro_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5911" evidence="2">
        <location>
            <begin position="9"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_hydro_15" evidence="1">
        <location>
            <begin position="222"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="597" mass="67359" checksum="90343A98B42C26B0" modified="2005-10-11" version="1">MIDHGLDLAVIGNCRTAALVDPTARLVWWCFPRFDSDPVFSRLLAGNEEKGFSDVVLDGMADFQSDYVRNTAIVSTVLTDTHGNAVRITDFAPRFRQYGRIFRPPQLFRIIEPVAGLPRITIRIRPTHAYGKPLARHSMGSNHIRYFEEDGTALRVTTDAPLALIENETPFVLTRPMYLVFGNDEAFPGDLASTAQRFAEETKTYWLSWVRRLYISYEWQEAIIRAAITLKLSNFEETGGIIAAHTTSIPEAPGSGRTWDYRYCWLRDAYFVVKALNRVGATRTMEDFIGFTLSLASNAEEDLKPVYGVVPNLPLNEWIAADLQGYHGDGPVRVGNAAVDQKQHDTYGSAILAALPMFFDRRLPRPGDESLFRLLESLGDKAARVAMTSDAGIWEYRGRQRIHTHSAAMCWAGINRLAAIAERLGLSDRAAHWNAIADPLHAELIERAWNPKREAFTAAFGSDDLDASVLLLPELGVCEVDDPRFVKTVAAMERELLREKHVMRYAAEDDFGLPVTAFLICRFWLIDAWWALGRREEAREAFTDALAHRNRFGLLSEDVDPKTGALFGNFPQTYSMAGLILTGMKLSRNWEDRYWRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN83</accession>
    <name>Q3SN83_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Probable ISRm2011-2 transposase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2949</name>
        <name evidence="4" type="ordered locus">Nwi_3008</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2005-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyripides N."/>
                <person name="Lykidis A."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>Nb-255</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
            <strain evidence="4">Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2949"/>
    <dbReference type="EnsemblBacteria" id="ABA06199">
        <property type="protein sequence ID" value="ABA06199"/>
        <property type="gene ID" value="Nwi_2949"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06258">
        <property type="protein sequence ID" value="ABA06258"/>
        <property type="gene ID" value="Nwi_3008"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2949"/>
    <dbReference type="KEGG" id="nwi:Nwi_3008"/>
    <dbReference type="eggNOG" id="COG3335">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056788_10_0_5"/>
    <dbReference type="OMA" id="ERACIHT"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0814">Transposable element</keyword>
    <feature type="domain" description="DDE_3" evidence="2">
        <location>
            <begin position="44"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA06258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="213" mass="24601" checksum="01FFF379FB9C8351" modified="2005-10-11" version="1">MGVRSRCEAQLQKKTLIAAEQDRPDVARRRAQWTKYRDRIDPTRLVFIDETWTKTNMAPLRGWAPRGQRIRAKVPHGRWQTMTFMAALRHDRITAPWFIEGPINGEAFLLYIEKVLVPTLRHGDIVIMDNLGSHKASAVRRVIRAAGARLFYLPKYSPDLNPIEQFFAKFKHWLRKATQRTTEAVYNAIPPILETVAPAECANYFVNAGYNQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SN89</accession>
    <name>Q3SN89_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_3002</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_3002"/>
    <dbReference type="EnsemblBacteria" id="ABA06252">
        <property type="protein sequence ID" value="ABA06252"/>
        <property type="gene ID" value="Nwi_3002"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_3002"/>
    <dbReference type="eggNOG" id="COG1618">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_890901_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018912">
        <property type="entry name" value="DUF2478"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10649">
        <property type="entry name" value="DUF2478"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="312" mass="34018" checksum="D4805CF2F7644EF7" modified="2005-10-11" version="1">MLSACSHPTVDPASWFRRFRIDATASGYSDHLTGCHRSVSLAFILFHDLPVASEAASIADREAALKQQAAAFSAPNTCRIYQRLRAGKRATTKTRTAIRTRTQDMADAEEETMEQRRAPHISAGSGPDTNPIGVVAYDDGLVRDALMAQCAADLATSGYRLGGIVQSNPHREGRRRCDMYVKDLLGGDEVKISQDRGNEARGCRLDQDAFARIDAWIERAVLEHVDLLIINKFGREEAQGRGLRAVIAEALIADIPVIIGVSSQNLADFLAFAGESATRLSPDIETMTAWCRNAIKRRAQDQCEPGLPPVGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNA2</accession>
    <name>Q3SNA2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2989</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316161.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06239">
        <property type="protein sequence ID" value="ABA06239"/>
        <property type="gene ID" value="Nwi_2989"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2989"/>
    <dbReference type="eggNOG" id="COG3812">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090929_1_0_5"/>
    <dbReference type="OMA" id="DMFPLVR"/>
    <dbReference type="OrthoDB" id="2020712at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034660">
        <property type="entry name" value="DinB/YfiT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018531">
        <property type="entry name" value="DUF1993"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36922">
        <property type="entry name" value="PTHR36922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09351">
        <property type="entry name" value="DUF1993"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109854">
        <property type="entry name" value="SSF109854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA06239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="167" mass="18723" checksum="CDE95992EAE198F4" modified="2005-10-11" version="1">MSFHDSASPAFLQMLGALSDILRKAEAHASARKIAPETLLTARLYPDMLPLTRQIQIACDFAVKTCARLSGSEVPSTPDTEKTFDELQQRIAKATDYVRKLPPDKFDGAEDREITFPAGRDQTMTLKGQQYLSHFALPNFYFHATTAYDILRHNGVELGKRDFMGVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNA9</accession>
    <name>Q3SNA9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GSDH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2982</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316154.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2982"/>
    <dbReference type="EnsemblBacteria" id="ABA06232">
        <property type="protein sequence ID" value="ABA06232"/>
        <property type="gene ID" value="Nwi_2982"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2982"/>
    <dbReference type="eggNOG" id="COG2133">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012253_1_1_5"/>
    <dbReference type="OMA" id="IWSKGHR"/>
    <dbReference type="OrthoDB" id="567019at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012938">
        <property type="entry name" value="Glc/Sorbosone_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011041">
        <property type="entry name" value="Quinoprot_gluc/sorb_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07995">
        <property type="entry name" value="GSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50952">
        <property type="entry name" value="SSF50952"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GSDH" evidence="1">
        <location>
            <begin position="50"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="382" mass="41453" checksum="C7738A2150E5AD7B" modified="2005-10-11" version="1">MKTPFTRATAILSATTILSAFFTTGARSESRSFRSSAGPIEVSTVASGLVHPWGLALLPDGRILVTERPGRFRIVSRDGDLSPPLKNVPGVWASGQGGLLDVVLDRSFADNKTLYFCFAERVDGGGRTAVARARLGEDRLQDVRIIFRQEGPLSSGNHYGCRIAQADDGNLFVTLGDHFSHRDEAQNLANHLGKVIRIAPDGSAPSDNPFVGRADARPEIWSYGHRNEQGLAIHPVTGGLWEVEHGPRGGDEVNIIGKGRNYGWPVIGYGVDYNGAKIHAGASREGMEQPVKYWAPSISPSSMAFYTGTLFPSWKGSLFIGALSGRMLVRLSLRGDEVIGEERLLQDLNERIRDVRQAPDGALWLLTDSKAGRLLRVSPSGK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-03" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNG2</accession>
    <name>RBL1B_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribulose bisphosphate carboxylase large chain 2</fullName>
            <shortName evidence="1">RuBisCO large subunit 2</shortName>
            <ecNumber evidence="1">4.1.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cbbL2</name>
        <name type="ordered locus">Nwi_2929</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 (2R)-3-phosphoglycerate + 2 H(+) = CO2 + D-ribulose 1,5-bisphosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:23124"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57870"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="EC" id="4.1.1.39"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-ribulose 1,5-bisphosphate + O2 = (2R)-3-phosphoglycerate + 2-phosphoglycolate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:36631"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57870"/>
            <dbReference type="ChEBI" id="CHEBI:58033"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterohexadecamer of 8 large chains and 8 small chains.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">The basic functional RuBisCO is composed of a large chain homodimer in a 'head-to-tail' conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric 'cap' on each end of the 'barrel'.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RuBisCO large chain family. Type I subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.39" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316104.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SNG2"/>
    <dbReference type="SMR" id="Q3SNG2"/>
    <dbReference type="STRING" id="323098.Nwi_2929"/>
    <dbReference type="EnsemblBacteria" id="ABA06179">
        <property type="protein sequence ID" value="ABA06179"/>
        <property type="gene ID" value="Nwi_2929"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2929"/>
    <dbReference type="eggNOG" id="COG1850">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031450_2_0_5"/>
    <dbReference type="OMA" id="IHGHPDG"/>
    <dbReference type="OrthoDB" id="848380at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016984">
        <property type="term" value="F:ribulose-bisphosphate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019253">
        <property type="term" value="P:reductive pentose-phosphate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08212">
        <property type="entry name" value="RuBisCO_large_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01338">
        <property type="entry name" value="RuBisCO_L_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033966">
        <property type="entry name" value="RuBisCO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020878">
        <property type="entry name" value="RuBisCo_large_chain_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000685">
        <property type="entry name" value="RuBisCO_lsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036376">
        <property type="entry name" value="RuBisCO_lsu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017443">
        <property type="entry name" value="RuBisCO_lsu_fd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036422">
        <property type="entry name" value="RuBisCO_lsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020888">
        <property type="entry name" value="RuBisCO_lsuI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42704">
        <property type="entry name" value="PTHR42704"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00016">
        <property type="entry name" value="RuBisCO_large"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02788">
        <property type="entry name" value="RuBisCO_large_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00014">
        <property type="entry name" value="RuBisCO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51649">
        <property type="entry name" value="SSF51649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54966">
        <property type="entry name" value="SSF54966"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00157">
        <property type="entry name" value="RUBISCO_LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0113">Calvin cycle</keyword>
    <keyword id="KW-0120">Carbon dioxide fixation</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0503">Monooxygenase</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000251452" description="Ribulose bisphosphate carboxylase large chain 2">
        <location>
            <begin position="1"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="298"/>
        </location>
    </feature>
    <feature type="binding site" description="in homodimeric partner" evidence="1">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="1">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="383"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="338"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="1">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01338"/>
        </source>
    </evidence>
    <sequence length="489" mass="54148" checksum="E7A0458BCD485A9E" modified="2005-10-11" version="1">MNVQNEKSMTVRGKDRYKSGVMSYKKMGYWEPDYTPKDTDVICLFRVTPQDGVDPIEASAAVAGESSTATWTVVWTDRLTAAEKYRAKCYRVDPVPGAEGQYFAYIAYDLDLFEPGSISNLTASVIGNVFGFKPLKALRLEDMRLPVAYVKTFKGPPTGIVVERERLDKFGRPLLGATVKPKLGLSGRNYGRVVYEALKGGLDFTKDDENINSQPFMHWRERFLYCMEAVNRAQAATGEIKGSYLNVTAATMEDMYERAEFAKELGSVVVMIDLVIGYTAIQSMSNWARKNDMILHLHRAGHSTYTRQRSHGVSFRVISKWMRLAGVDHIHAGTVVGKLEGDPLTTRGFYDICREEYNPTQLEHGIFFDQNWASLNKVMPVASGGIHAGQMHQLIQHLGEDVVLQFGGGTIGHPMGIQAGATANRVALEAMILARNEGRDYVSEGPEILAKAAASCTPLKQALEVWKDVTFDYASTDAPDYVPTAVPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNH2</accession>
    <name>Q3SNH2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase, IS4 family</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ordered locus">Nwi_2919</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA06169">
        <property type="protein sequence ID" value="ABA06169"/>
        <property type="gene ID" value="Nwi_2919"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2919"/>
    <dbReference type="eggNOG" id="COG3293">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055261_9_1_5"/>
    <dbReference type="OMA" id="ALERQWC"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE_Tnp_1" evidence="2">
        <location>
            <begin position="54"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="210" mass="23869" checksum="21813CD92411404D" modified="2005-10-11" version="1">MGGHSSALRPAHHLREPLQPVAQGRRVGSHSVGCFKGLRWRHPNDRFVVDPRASTCGQRSKKQTRSRCMGRSRGGLTTKIHALVDACGLPIVLRITEGQAHDGRSAQDMIDTVERGDVLLADRAYDSNALRQTLAARGARANVKPMPNRVAALQFNRRLYRKRNLVERFFNKIKHYRAVATRYDKRDDNFLTSIKLASIRIWLRFNESMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNJ5</accession>
    <name>Q3SNJ5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Diguanylate cyclase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2896</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2896"/>
    <dbReference type="EnsemblBacteria" id="ABA06146">
        <property type="protein sequence ID" value="ABA06146"/>
        <property type="gene ID" value="Nwi_2896"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2896"/>
    <dbReference type="eggNOG" id="COG2199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_11_36_5"/>
    <dbReference type="OMA" id="YCAGTTT"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="2">
        <location>
            <begin position="235"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA06146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="393" mass="42962" checksum="F27683CB73E24412" modified="2005-10-11" version="1">MPFRTETVRDGVYVELVRSLYTTLIPTTIMSIGYVLSLSVMAFEAGDRVLAVFSFIGLLSNAARIWILIGGRAEAAASDLEITRARALEKRFALAYYQFAVVLGVSAAYVFMLDPARFHMLMICLLVGYGAGAATGVGLRPRIAITSMLVAIVPTILVAAWHHEPIYWITAAMTSALLAGGCQSLLARWRVTSTEIARRLTFEALARRDVLTTLPNRLALREWFDSHIAVGSGQRLLAVHCLDLDNFKPVNDVFGHPVGDGLLKAIAMRLIQTLRPGDIVARLGGDEFAVIQRDLHDPDEAAAQAQQFRAAIAEPFLVHGHEISVTTCVGYALCYCGAADLDELLGLADQALYIAKNTDTGVEYDDSLLRSALRMDDWYESSRSQSQSAHPMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNT3</accession>
    <name>Q3SNT3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2805</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011316003.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2805"/>
    <dbReference type="EnsemblBacteria" id="ABA06058">
        <property type="protein sequence ID" value="ABA06058"/>
        <property type="gene ID" value="Nwi_2805"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2805"/>
    <dbReference type="eggNOG" id="ENOG502ZXE6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1640561_0_0_5"/>
    <dbReference type="OMA" id="CYLGLIP"/>
    <dbReference type="OrthoDB" id="2065446at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA06058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="158" mass="16828" checksum="9BD4997682BE40A8" modified="2005-10-11" version="1">MALIGRLFAILFGLLAACFVAGTIVVVALLYPEFSDLDVTLPNPDTLDVVLGFGFIFVSGFALLPAMIVVLITEAFSIRGILAYAVGGAICGAACYLGLVPFDPETMRFDGIVRRHLEIMTGAGVVAGLTYWLIAGRNAGAWREAPRDPRPPDLPSRT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SNY6</accession>
    <name>Y2752_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0301 protein Nwi_2752</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">Nwi_2752</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0301 (AlgH) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA06005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SNY6"/>
    <dbReference type="SMR" id="Q3SNY6"/>
    <dbReference type="STRING" id="323098.Nwi_2752"/>
    <dbReference type="EnsemblBacteria" id="ABA06005">
        <property type="protein sequence ID" value="ABA06005"/>
        <property type="gene ID" value="Nwi_2752"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2752"/>
    <dbReference type="eggNOG" id="COG1678">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057596_1_0_5"/>
    <dbReference type="OMA" id="IYWGGDF"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00758">
        <property type="entry name" value="UPF0301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003774">
        <property type="entry name" value="UPF0301"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30327">
        <property type="entry name" value="PTHR30327"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02622">
        <property type="entry name" value="DUF179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000258846" description="UPF0301 protein Nwi_2752">
        <location>
            <begin position="1"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00758"/>
        </source>
    </evidence>
    <sequence length="221" mass="24099" checksum="20F6D6C36B9D0601" modified="2005-10-11" version="1">MKPARKGPGRDRRHADDADMDIADTDTYDRNYLDGQLLIAMPVMEDERFARSVIYVCAHSSEGAMGIILNRPAGSVDFSDLLVQLDIIKRADLIKLPETAETMKVMKGGPVETGRGFVLHSSDFFIEDATLPIDEGICLTATLDILEAIAKGAGPKHAILALGYAGWAPGQLETEIQDNGWLHCPADQDLIFGRDIEDKYVRALHKIGIDPGMLSNEAGHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SP96</accession>
    <name>Q3SP96_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">6-phosphogluconate dehydrogenase (Decarboxylating)</fullName>
            <ecNumber evidence="6">1.1.1.44</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_2642</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; pentose phosphate pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 6-phosphogluconate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.44" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315845.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2642"/>
    <dbReference type="EnsemblBacteria" id="ABA05895">
        <property type="protein sequence ID" value="ABA05895"/>
        <property type="gene ID" value="Nwi_2642"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2642"/>
    <dbReference type="eggNOG" id="COG1023">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024540_0_0_5"/>
    <dbReference type="OMA" id="NGYALMY"/>
    <dbReference type="OrthoDB" id="1245550at2"/>
    <dbReference type="UniPathway" id="UPA00115"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004616">
        <property type="term" value="F:phosphogluconate dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019521">
        <property type="term" value="P:D-gluconate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016054">
        <property type="term" value="P:organic acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004849">
        <property type="entry name" value="6DGDH_YqeC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006114">
        <property type="entry name" value="6PGDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006183">
        <property type="entry name" value="Pgluconate_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11811">
        <property type="entry name" value="PTHR11811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00393">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00076">
        <property type="entry name" value="6PGDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01350">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00872">
        <property type="entry name" value="gnd_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0311">Gluconate utilization</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6PGD" evidence="5">
        <location>
            <begin position="186"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="317"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="338" mass="36643" checksum="767037D24333B832" modified="2005-10-11" version="1">MQIGMIGLGRMGANIVRRLMKKGGHHAFVYDISEQAVAALAAEGATAATDLEDFVRKLEKPRAVWIMLPAGAVTESTIATIAGLLDGDDVIIDGGNTFWQDDIRRAGTLREKGIHYVDVGTSGGVWGFERGYCMMIGGERAVVDRLDPIFAALAPGHGDIPKTPSRDGRDPRVEQGYLHTGPVGSGHFVKMVHNGIEYGLMQAYAEGFDILRNANVPALPEQHRFKLDLADIAEVWRRGSVISSWLLDLTASALARSETLEAYSGFVADSGEGRWTVNAAIDEAVPAEVLTAALFARFRSRQQHTFAEKILSAMREGFGGHKEPGEADPSTKERTANR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPB6</accession>
    <name>Q3SPB6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Polynucleotide adenylyltransferase region</fullName>
            <ecNumber evidence="6">2.7.7.72</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_2622</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.72" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315825.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2622"/>
    <dbReference type="EnsemblBacteria" id="ABA05875">
        <property type="protein sequence ID" value="ABA05875"/>
        <property type="gene ID" value="Nwi_2622"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2622"/>
    <dbReference type="eggNOG" id="COG0617">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015961_2_3_5"/>
    <dbReference type="OMA" id="YNYELRI"/>
    <dbReference type="OrthoDB" id="2018439at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052929">
        <property type="term" value="F:ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052928">
        <property type="term" value="F:CTP:3'-cytidine-tRNA cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052927">
        <property type="term" value="F:CTP:tRNA cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016437">
        <property type="term" value="F:tRNA cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd05398">
        <property type="entry name" value="NT_ClassII-CCAase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002646">
        <property type="entry name" value="PolA_pol_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032828">
        <property type="entry name" value="PolyA_RNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01743">
        <property type="entry name" value="PolyA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12627">
        <property type="entry name" value="PolyA_pol_RNAbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PolyA_pol" evidence="4">
        <location>
            <begin position="32"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="PolyA_pol_RNAbd" evidence="5">
        <location>
            <begin position="200"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="418" mass="45436" checksum="55007707542063D1" modified="2005-10-11" version="1">MNAARSLCDAGWLKSGPAVRVLSLLGGNGEEARVVGGAVRNALMGLPVVEIDIATTAPPEEVMRRAAAARIRSIPTGVEHGTVTLVVEGKPVEVTTLREDVETFGRKARVAFGRDWARDAQRRDFTINGLSVDSGGTVHDHVGGLDDIAARRVRFIGDPDRRIAEDYLRILRFFRIHAAYGAGEPDRDGYLACIRGRAGLATLSAERLRAEILKLLLADGAVEAAVAMEDGGLLLAVFGGVAYRGPLAAMMAAESALELAPDPIRRLAALAVSVTEDARRLAQRLRLSNAEAKQLDSMGHRWWRLGSMDEATARRRLYRLGATRFRDRLMQAWSKAGLGADPAPWLNVATLPRRWQPPAFPLKAEDFIVRGIAEGPALGHVLTLAEDAWLAEDFPLDSARLSAIADQTVARFTRDHRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPF5</accession>
    <name>Q3SPF5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, TetR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2583</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315787.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2583"/>
    <dbReference type="EnsemblBacteria" id="ABA05836">
        <property type="protein sequence ID" value="ABA05836"/>
        <property type="gene ID" value="Nwi_2583"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2583"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_12_0_5"/>
    <dbReference type="OMA" id="HVLSHRK"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="14"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="226" mass="23459" checksum="30C64E9033DED543" modified="2005-10-11" version="1">MVYRRTQQVMKRLAARRRAILTAARDIAGNAGMAAVQIAPVALRAEVAAGTVYRYFPSKADLISELIAEVVRDELTAIRGAADAAPGPSSALAAAIATVAVHMVSHRKLAWGILAEPVDVDVAASRLASRRDIVGEIETRIKAAVSAGHLPQQDTSLAATALLGALHEALVGPLAPDFEDQASLRDVVKAATLLALRAVGVTDARARGLVVQAALPATTPAGNKTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SPW1</accession>
    <name>Q3SPW1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nicotinate-nucleotide diphosphorylase (carboxylating)</fullName>
            <ecNumber evidence="4">2.4.2.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Quinolinate phosphoribosyltransferase [decarboxylating]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Nwi_2426</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the catabolism of quinolinic acid (QA).</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from quinolinate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the NadC/ModD family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.19" evidence="4"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315638.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2426"/>
    <dbReference type="EnsemblBacteria" id="ABA05680">
        <property type="protein sequence ID" value="ABA05680"/>
        <property type="gene ID" value="Nwi_2426"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2426"/>
    <dbReference type="eggNOG" id="COG0157">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039622_0_1_5"/>
    <dbReference type="OMA" id="DMIMLKD"/>
    <dbReference type="OrthoDB" id="1718114at2"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00331"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004514">
        <property type="term" value="F:nicotinate-nucleotide diphosphorylase (carboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01572">
        <property type="entry name" value="QPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004393">
        <property type="entry name" value="NadC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027277">
        <property type="entry name" value="NadC/ModD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037128">
        <property type="entry name" value="Quinolinate_PRibosylTase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002638">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022412">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32179">
        <property type="entry name" value="PTHR32179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01729">
        <property type="entry name" value="QRPTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02749">
        <property type="entry name" value="QRPTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006250">
        <property type="entry name" value="NadC_ModD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="SSF51690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00078">
        <property type="entry name" value="nadC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="QRPTase_N" evidence="11">
        <location>
            <begin position="26"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="QRPTase_C" evidence="10">
        <location>
            <begin position="115"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR006250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABA05680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="287" mass="30003" checksum="91F2B7494E14B7BF" modified="2005-10-11" version="1">MPLPALPRIMIEPLVRAALLEDLGRAGDLTTDAIVPVGHRTTAVIAARQQGTVAGLDLARLAFELIDPAIEMHAGHDDGAIVAPGDVIATLSGPARGILTAERVALNFLCHLSGIATATASVVAAVQDYKAKIVCTRKTTPGLRAIEKYAVRAGGGGNHRFGLDDAVLIKDNHIVIAGGVTEALKRAKDRVGHLVKIELEVDTLHQLREALEQGVDAVLLDNMSPDTLREAVAMVAGRAVTEASGRITPATAPAIASSGVDLMSIGWLTHSVGILDLGLDFEDEVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ76</accession>
    <name>Q3SQ76_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_2311</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315530.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2311"/>
    <dbReference type="EnsemblBacteria" id="ABA05565">
        <property type="protein sequence ID" value="ABA05565"/>
        <property type="gene ID" value="Nwi_2311"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2311"/>
    <dbReference type="eggNOG" id="COG3336">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054944_0_1_5"/>
    <dbReference type="OMA" id="WAMELSL"/>
    <dbReference type="OrthoDB" id="1553593at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019108">
        <property type="entry name" value="Caa3_assmbl_CtaG-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09678">
        <property type="entry name" value="Caa3_CtaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="110"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="169"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA05565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="254" mass="27297" checksum="E715A57F2B481108" modified="2005-10-11" version="1">MRQGADPYCGPAPLPEDLASSWNFDPLLIAVFAGVAAACMMMSRERRIAALKSPAALAGWAVLLAAFVSPLCALASALFSARVFHHVLLVALAAPLFALAFPPRATLRRVPVSGLVLIHAICMWVWHTPGPYAFALDSTAAYWMMEASLFLSAFLLWQAVLDRRNHAGAALAALLATVIQMGMLGALLTFAARPLFDHHMTTTLPYGLTPLDDQQLAGLLMWIPASMSYLAAAALLAARMLETHPHPTQSTRPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ98</accession>
    <name>Q3SQ98_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">HpcH/HpaI aldolase</fullName>
            <ecNumber evidence="7">4.1.3.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2289</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HpcH/HpaI aldolase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.6" evidence="7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315510.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2289"/>
    <dbReference type="EnsemblBacteria" id="ABA05543">
        <property type="protein sequence ID" value="ABA05543"/>
        <property type="gene ID" value="Nwi_2289"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2289"/>
    <dbReference type="eggNOG" id="COG2301">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044864_0_2_5"/>
    <dbReference type="OMA" id="AWLFCPA"/>
    <dbReference type="OrthoDB" id="1107373at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008815">
        <property type="term" value="F:citrate (pro-3S)-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005000">
        <property type="entry name" value="Aldolase/citrate-lyase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011206">
        <property type="entry name" value="Citrate_lyase_beta/mcl1/mcl2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03328">
        <property type="entry name" value="HpcH_HpaI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015582">
        <property type="entry name" value="Cit_lyase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HpcH_HpaI" evidence="6">
        <location>
            <begin position="4"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 5">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR015582-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR015582-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="293" mass="30833" checksum="B9EE8FB01832EC66" modified="2005-10-11" version="1">MKWRSLLFVPADSDRKFARAGTCGADALVLDLEDSVAPGCKEVAREAVKSFLGGATDRDWTFLVRINALGTGLTLADLAAVVRPGLDGILIPKVNGVEDIRQISHYVDVLEMAAGIPTGHVKLLAVVTETPAAMLGFAGYAAQGPGQVSRLAAMTWGAEDLSAAVGALTNKEPNGDWTFPYQVARAQCLFAANAAGVVALDTLYSDFRDQAGLAESCRVARRDGFAGRLAIHPDQVATINACFTPTETDLAHARRVVAAFASQPDAGVIGIEGRMYDIPHLIAAKRTLASAGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQ99</accession>
    <name>Q3SQ99_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2288</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315509.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2288"/>
    <dbReference type="EnsemblBacteria" id="ABA05542">
        <property type="protein sequence ID" value="ABA05542"/>
        <property type="gene ID" value="Nwi_2288"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2288"/>
    <dbReference type="eggNOG" id="COG1680">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020027_11_2_5"/>
    <dbReference type="OMA" id="AGWAVRY"/>
    <dbReference type="OrthoDB" id="1439085at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase" evidence="1">
        <location>
            <begin position="29"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="424" mass="45716" checksum="4514F8CA8997BD7B" modified="2005-10-11" version="1">MTTHNSGSPEASGMSSVALARIDDHLKRRYIDAGRFPGTQLMVYRRGSLVHHSVQGFADLERKVRLKGDAIFRIYSMTKPITSVAFMMLVEQGLVALDDPVHRYIPEWKNLGVFQAGAAPAFMTRPPSRPMQIVDLLRHTSGLTYGFQQRTNVDAAYRDKRIGTLDPEGTLASMIADLAGIPLEFSPGEAWNYSVSTDVIGYLIGRISGIPFDRYLKERIFDPLEMADTGFFVPAGKAHRLAACYSADAGGMVPARSPGATSGLTLQDDPATSPFLSPPGFVSGGGGLCSTATDYLTFCRALLNGGELGGVRLIGPKTLALMTSNHLPGNRDLPEMSRSMFAEATYSGIGFGLGFAVTINPAKTLIAGSPGEYAWGGVATTSFWIDPAEELIAIFMTQVIPSTAYPVRRELRTMVYAAITDSNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQF5</accession>
    <name>Q3SQF5_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4332 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2232</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315454.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2232"/>
    <dbReference type="EnsemblBacteria" id="ABA05486">
        <property type="protein sequence ID" value="ABA05486"/>
        <property type="gene ID" value="Nwi_2232"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2232"/>
    <dbReference type="eggNOG" id="COG3743">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117602_1_0_5"/>
    <dbReference type="OMA" id="GTQYCGL"/>
    <dbReference type="OrthoDB" id="1642788at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025567">
        <property type="entry name" value="DUF4332"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14229">
        <property type="entry name" value="DUF4332"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4332" evidence="3">
        <location>
            <begin position="9"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="135" mass="14784" checksum="ED4E155BBF094E14" modified="2005-10-11" version="1">MTYSISKIEGLSAFEAKKLRSIGIRTTDAYLEAARTSKGRKALAARTGISEQQLLEWANLADYMRIPGMGNGKTKLLRAAGVTTVRELTHRNPARLAQAMKDANAKRKLVSALPSEKSIGDLIAQARKLPLKITY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQH8</accession>
    <name>Q3SQH8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Amino acid/amide ABC transporter membrane protein 1, HAAT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Nwi_2209</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315432.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2209"/>
    <dbReference type="EnsemblBacteria" id="ABA05463">
        <property type="protein sequence ID" value="ABA05463"/>
        <property type="gene ID" value="Nwi_2209"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2209"/>
    <dbReference type="eggNOG" id="COG0559">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039929_3_0_5"/>
    <dbReference type="OMA" id="YIVGCFM"/>
    <dbReference type="OrthoDB" id="1529037at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="279"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA05463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="304" mass="32717" checksum="78753445C99AFB8A" modified="2005-10-11" version="1">MDYFAQQLINGVVLGSIYGLIATGYTMVYGIVGMINFAHGDIFMIGGFIALITFLILMSMGLSAVPLILLVMLIVSMAVTAVYGWVVERIAYRPLRQSFRLAPLLSAIGMSFVLMNFSQVSQGAQVKPIPPLITGGYTLHDSGNFAVQLSNVQIVVVTTTIVLLAIFSYIVSRTRFGRDMRACEQDQTMAALLGVNVDRTISMTFVIGASLAAVAGMMYLLYYGLVDFFMGFVAGIKAFTAAVLGGIGSLPGAMLGGLAIGLIETFWSAYFSIEYKDVAAFSILIIVLIFMPTGLLGRPEVEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="127" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQJ6</accession>
    <name>Q3SQJ6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Co-chaperonin GroES</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">10 kDa chaperonin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Chaperonin-10</fullName>
            <shortName evidence="3">Cpn10</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">groES</name>
        <name evidence="3" type="synonym">groS</name>
        <name evidence="5" type="ordered locus">Nwi_2191</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Together with the chaperonin GroEL, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding. GroES binds to the apical surface of the GroEL ring, thereby capping the opening of the GroEL channel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heptamer of 7 subunits arranged in a ring. Interacts with the chaperonin GroEL.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3 4">Belongs to the GroES chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315414.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2191"/>
    <dbReference type="EnsemblBacteria" id="ABA05445">
        <property type="protein sequence ID" value="ABA05445"/>
        <property type="gene ID" value="Nwi_2191"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2191"/>
    <dbReference type="eggNOG" id="COG0234">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132825_1_0_5"/>
    <dbReference type="OMA" id="EVKYSGE"/>
    <dbReference type="OrthoDB" id="1965002at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00320">
        <property type="entry name" value="cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.33.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00580">
        <property type="entry name" value="CH10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020818">
        <property type="entry name" value="Chaperonin_GroES"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037124">
        <property type="entry name" value="Chaperonin_GroES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018369">
        <property type="entry name" value="Chaprnonin_Cpn10_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772">
        <property type="entry name" value="PTHR10772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00166">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00297">
        <property type="entry name" value="CHAPERONIN10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00883">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00681">
        <property type="entry name" value="CHAPERONINS_CPN10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA05445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="105" mass="11275" checksum="7AB8B70C6268E1D6" modified="2005-10-11" version="1">MKFRPLHDRVVVKRIDAEEKTAGGIIIPDTAKEKPSQGEVVAVGPGGRDEAGKLIPIDLKVGDRVLFGKWSGTEVKIEGQDLLIMKESDIMGVLTDVAPAKKKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQL0</accession>
    <name>Q3SQL0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2177</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315400.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2177"/>
    <dbReference type="EnsemblBacteria" id="ABA05431">
        <property type="protein sequence ID" value="ABA05431"/>
        <property type="gene ID" value="Nwi_2177"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2177"/>
    <dbReference type="eggNOG" id="ENOG502ZAVI">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029427_0_0_5"/>
    <dbReference type="OMA" id="GYLARWY"/>
    <dbReference type="OrthoDB" id="1851238at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="393"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="422"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="450"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="490" mass="53991" checksum="FCB6DA072FA341CF" modified="2005-10-11" version="1">MHLRRTLNRRNGGIGVTAGRATGGTRISTRVLYRRSMEAPWAWIAAVIIPILLLAPAIWNGYPLLQHDTGGYLARWYESHLEISRSNAYGIYLHLGQGSYFWINLACQALLTLWILHLTLRVLNMAQPLRLLTIALILIAATALPWLASMLLTDIFAGLAVLTLYILVAHNGKLSIAERISLFLFTSFAAASHTATFGVLLGLCCAGWIVKPLLGPRLQTGGLIQGSLTIVTGAAMLLAANFTLTGQLTWTPGGAAVSFGRMMQDGIVARYLNDHCDKIKLKLCPYRDQLPATADDFLWGNDSIFDKLGRFEGMNGEMEFIATHALVAYPLWQAEAAVEATAEQLVSVDTGEGNTGWIPHTYGIIERYLPEQVKTMRDARQQHWNMKFTAINWIHVPVALASMLLLAAIALAALWRRRLDDVDLLAVTVTVALLGNAFICGVLSGPHDRYGARMVWVATFVVLIAAARFLAHRHDPPYGRRDKNFSTPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQL4</accession>
    <name>Q3SQL4_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2173</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315396.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2173"/>
    <dbReference type="EnsemblBacteria" id="ABA05427">
        <property type="protein sequence ID" value="ABA05427"/>
        <property type="gene ID" value="Nwi_2173"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2173"/>
    <dbReference type="eggNOG" id="ENOG5032V60">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_173788_0_0_5"/>
    <dbReference type="OMA" id="WSMMSGT"/>
    <dbReference type="OrthoDB" id="1784469at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="109" mass="11717" checksum="A7A43131B80EDA10" modified="2005-10-11" version="1">MSPNALNSFFSLCIGFAIAGALASGYQALVKRPAGFGLLQQGVVARAFAAVPFLVFAAPFIIMRNTLRGRRIERRRFEFVMMATVVAGFWSLMSGTCVVLTLKAVGLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQP3</accession>
    <name>Q3SQP3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">tRNA and rRNA cytosine-C5-methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_2144</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315367.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2144"/>
    <dbReference type="EnsemblBacteria" id="ABA05398">
        <property type="protein sequence ID" value="ABA05398"/>
        <property type="gene ID" value="Nwi_2144"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2144"/>
    <dbReference type="eggNOG" id="COG0144">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005316_0_2_5"/>
    <dbReference type="OMA" id="PDAKWKL"/>
    <dbReference type="OrthoDB" id="1064993at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB/NOP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="PTHR22807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM_MT_RSMB_NOP" evidence="5">
        <location>
            <begin position="145"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="361"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA05398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="432" mass="46562" checksum="71E336314905AA81" modified="2005-10-11" version="1">MTPSARLSAAIEVIEAIDNQRIPAAKALKEWGTAHRFAGSGDRAAISGLVYDVLRRQASSAWIMDDDTPRARLLGTLKLERGINTDAIASLCDGGRFAPEPLTEGERQALASRSLGDAPAHIAGDYPQWLDVYLDEAFGDDRAAEAAAMACRAPLDLRINTLKAKRDKVLGSLQHLGVTATPWSPLGLRIMLGADARNPGIQAEEEFIKGAVEVQDEGSQIAALLSGARPGEQVIDLCAGAGGKTLTLAAMMQGRGRLIATDRDKRQLAPIHERLSRAGVHNAEVRTPKGEDDPLSDIRASADLVLIDAPCTGTGTWRRNPDAKWRMRPGALDIRLKDQAEVLDRASALVKPGGRVVYITCSVLPQENSGQIRKFIGRHPDFSVIPPSPTASVLWDRTDDFLAAVYASDEGLLMTPRRTGTDGFFVSILTKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQR3</accession>
    <name>Q3SQR3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ParB family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2123</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315349.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2123"/>
    <dbReference type="EnsemblBacteria" id="ABA05378">
        <property type="protein sequence ID" value="ABA05378"/>
        <property type="gene ID" value="Nwi_2123"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2123"/>
    <dbReference type="eggNOG" id="COG1475">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019174_1_0_5"/>
    <dbReference type="OMA" id="FERPNPY"/>
    <dbReference type="OrthoDB" id="1375763at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB/Sulfiredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02195">
        <property type="entry name" value="ParBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="SSF110849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ParB" evidence="3">
        <location>
            <begin position="16"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="663"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="312"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="690"/>
            <end position="706"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="717" mass="79688" checksum="2C02210E9F9EDA13" modified="2005-10-11" version="1">MAKAARKIVFSRSRDIPFNKLVLSESNVRRTRPEAELDELVHDIDRREDLVQGLNVRAILDADGNETGMFEVPAGGRRYRAIERLVKAKRFPKDGLVPCIVRKSDTRILAEDDSLAENLLRAGLHPLDQFRAFQDMLDNGMTEEEIAAAYLTTVQVVKQRLRLNAVSPVLRDAYAEERMTLDMLMAFTVNPDHQSQEQVWEAVQHSYNRQPWHIRQLLTETTIPASDKRARFVGIDAYVAAGGAVLRDLFEDDDGGWLADPALLHRLVSEKLKAVADEIAAEGWKWIKIDLDLPYGYDHGLRQIGGTFVDLTEKERAEREALRAEYDRLEAEYDGADELPDEIDQRLGEIEEALEAFEKRPVVYDPADIARAGVFVSVDRDGDIVVDRGYVRPEDEAPVAVNGSGAEVVREPDAVDAPATPSAQRAVITIRGQGTDADDDEEDDAIKPLPERLVIELTAHRTLALRDAVANHPQVALTALLHKLVLDAFKRNAHGCAVEASVREVHFPVQATDLKDSASAKAIQARMDAWKADMPLEDDDRLWNWIAGLDDASRLALLAHCVSYGINALYERPNPHSGYGVTQHGLDRRMRDADRLARATSLDMVEAGWRPTVANYLGRVTKSRIIEAVREALGEEKAQLIDHLKKPEMAKEAERLLADTGWLPEPLRSADPDATTSVDVSGGEGDETLPAFLADDEDEQADDGEGDADDRAMIAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQT6</accession>
    <name>Q3SQT6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Conjugal transfer protein trbE</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_2098</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TrbE/VirB4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2098"/>
    <dbReference type="EnsemblBacteria" id="ABA05355">
        <property type="protein sequence ID" value="ABA05355"/>
        <property type="gene ID" value="Nwi_2098"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2098"/>
    <dbReference type="eggNOG" id="COG3451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_686655_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018145">
        <property type="entry name" value="CagE_TrbE_VirB_cntrl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03135">
        <property type="entry name" value="CagE_TrbE_VirB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CagE_TrbE_VirB" evidence="3">
        <location>
            <begin position="28"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="382"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="401" mass="43396" checksum="55CE92F0667F2A3E" modified="2005-10-11" version="1">MLDKTDATRLVTKIRRQWFAKRKSVAAILKEVMTNEQSVLLDTDAANKAADADAALQDLGSDQVGEVYVTATVMVWSADRTIADERLRLVEKVIQSRDFTCIVEGVNAIEAWLGSIPGQTYANVRQPPISTLNLAHMMPLSAVWAGPARDEHLGAPPLFYAKTEGATPFRFALHVGDVGHTMIVGPTGAGKSVLLALMALQFRRYRNSQVFVFDFGGSIRAATLAMGGDWQDLGGTLTDDAESTSVSLQPLAFIHHVLERAWAADWLVDILLREGVTVTPDVKEYIWAALTSLASAPVEERTITGLTVLLQSSALKQALRPYCIGGAHGRLLDAEHEYLGTANVQAFETEGLVGTKAAPAVLSYLFHRIEARFDGRRACSSSTRAGARSMTAASPTRSRSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQT8</accession>
    <name>Q3SQT8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Possible conjugal transfer protein TrbJ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_2096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315331.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2096"/>
    <dbReference type="EnsemblBacteria" id="ABA05353">
        <property type="protein sequence ID" value="ABA05353"/>
        <property type="gene ID" value="Nwi_2096"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2096"/>
    <dbReference type="eggNOG" id="COG5314">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065326_1_0_5"/>
    <dbReference type="OMA" id="CPFAPAV"/>
    <dbReference type="OrthoDB" id="1557327at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014147">
        <property type="entry name" value="T4SS_TrbJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02780">
        <property type="entry name" value="TrbJ_Ti"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229207" evidence="1">
        <location>
            <begin position="33"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="249" mass="26680" checksum="71239B6A50CD1E28" modified="2005-10-11" version="1">MILSVSRSRYLSSIAAGALAIGIALSSAPASAQIVFDPSNYAQNVLTAARALQQINNQITSLANQAQMLMNQAKQIASLPTSVLSQIEGNFTEMKRLMGEAERLAYNVSNIESQFSSTFQNFAVGKSDAQLIASARERWQQSLSAYEHSLKAGATAVQNLDSTRTQTSTLVNTSQSAVGVLQATQAGNQLLGVQTSQLADLTPMLAAQGRANALEKSRQAAAQEQAREQFGRFLTGSSYTPTTVRMFHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQX5</accession>
    <name>Q3SQX5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2058</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2058"/>
    <dbReference type="EnsemblBacteria" id="ABA05316">
        <property type="protein sequence ID" value="ABA05316"/>
        <property type="gene ID" value="Nwi_2058"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2058"/>
    <dbReference type="eggNOG" id="ENOG5032PAR">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2317400_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="110" mass="12372" checksum="C8C3852919F34D1D" modified="2005-10-11" version="1">MPLSTRTEAKHMKDLKLYLDKLLADSEHCITISKSTLNDKKREVFEMLAATYRKLASDIETVMATNAILDEERDKRLIGLLGTDDNAADSITEITKLLGQKPEKPTPPEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQX8</accession>
    <name>Q3SQX8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase_M15_4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_2055</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315294.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05313">
        <property type="protein sequence ID" value="ABA05313"/>
        <property type="gene ID" value="Nwi_2055"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2055"/>
    <dbReference type="HOGENOM" id="CLU_1276515_0_0_5"/>
    <dbReference type="OMA" id="ADSGHFQ"/>
    <dbReference type="OrthoDB" id="2120776at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009045">
        <property type="entry name" value="Hedgehog_sig/DD-Pept_Zn-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039561">
        <property type="entry name" value="Peptidase_M15C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13539">
        <property type="entry name" value="Peptidase_M15_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55166">
        <property type="entry name" value="SSF55166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229263" description="Peptidase_M15_4 domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M15_4" evidence="2">
        <location>
            <begin position="137"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA05313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="216" mass="23643" checksum="60F0F583CBAAF678" modified="2005-10-11" version="1">MIRLLIASAMICIVATPSSARSFSKKWQTAQGCDAMRPCHGDWSALYGSAQHPHGHVRRYAVRHRGRHSHRRIPSGLARVMAEGAGRAVGVVRSTSGATARVANRATGAFQCLVSALDRQGYPIRFMGGWRAHGSVRGSLHPAGLALDINQYGRNVTRPRMPRNEIALANSCGLIAGAQWSNADSGHFQLGGWSGKGRHETRRRYHRGARYANARW</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SQY9</accession>
    <name>Q3SQY9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_2043</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_2043"/>
    <dbReference type="EnsemblBacteria" id="ABA05302">
        <property type="protein sequence ID" value="ABA05302"/>
        <property type="gene ID" value="Nwi_2043"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_2043"/>
    <dbReference type="eggNOG" id="ENOG5032Z13">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148087_0_0_5"/>
    <dbReference type="OMA" id="HNPMCEC"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA05302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="108" mass="11544" checksum="DFEE52E3DE9C5DC4" modified="2005-10-11" version="1">MMGLRKGGLTIAVIGAVSLFIGAAWAHPPFCICKMANATTVSCEGGFADGTGVPGVRLDVIRYDESIVLETKFGPDSTVSFKRPDGEFYMLFDAGPGQSFDVDYKDIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR36</accession>
    <name>Q3SR36_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1996</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA05255">
        <property type="protein sequence ID" value="ABA05255"/>
        <property type="gene ID" value="Nwi_1996"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1996"/>
    <dbReference type="HOGENOM" id="CLU_205282_0_0_5"/>
    <dbReference type="OMA" id="CMQRRSH"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA05255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="67" mass="7510" checksum="FAA27349EED1E9FF" modified="2005-10-11" version="1">MCISIPECMQRRSHLTLEGNMAKKAKKAKKAKAKAKTTKKVKKTKSAVKKTAKKTRKVAKKVAKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SR40</accession>
    <name>Q3SR40_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">NADH/Ubiquinone/plastoquinone (Complex I)</fullName>
            <ecNumber evidence="9">1.6.99.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Nwi_1992</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Membrane</location>
            <topology evidence="1 5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.6.99.5" evidence="9"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1992"/>
    <dbReference type="EnsemblBacteria" id="ABA05251">
        <property type="protein sequence ID" value="ABA05251"/>
        <property type="gene ID" value="Nwi_1992"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1992"/>
    <dbReference type="eggNOG" id="COG1009">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007100_11_0_5"/>
    <dbReference type="OMA" id="GYSTMGQ"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_mem"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003945">
        <property type="entry name" value="NU5C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001516">
        <property type="entry name" value="Proton_antipo_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42829">
        <property type="entry name" value="PTHR42829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00662">
        <property type="entry name" value="Proton_antipo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0830">Ubiquinone</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="247"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="317"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="382"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="415"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="444"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="478"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="Proton_antipo_N" evidence="8">
        <location>
            <begin position="73"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Proton_antipo_M" evidence="7">
        <location>
            <begin position="128"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABA05251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="566" mass="61559" checksum="97BA09F8971A0BE6" modified="2005-10-11" version="1">MDQWVSWCLVVLPALPVLSAIMAPLAGSGQQARAARLSVAMTAVTCVVSVALLVAVLRAPQGDVTVARLGPAALMLDNLAVAMAVLVSAISLVVHVYSVRYMADEPGYVRFFAMLDLMTAVILLMVTAADLLTLLAAWFLIGVLLFFLLGFDTSRKASGRYALWTFLTYRVGDLPMVGAALLLWHGYGTAELSELFARITANPDVQLGGVAVVPLVAMLLALAAFARSAQFLLHTWLPYTMDGPTPVSALMHAGIVNAGGFLFNRFAPVFVHAPDALHLAFVVGLVTALIGSALMLTQNDVKKSLGYSTMGQMGFMIMECGLGAFSLAVYHLIAHGLFKATLFLNAGNVIGDSRREDGVPPDDIYSFVVERRPLREVQKVPWLWAALATVLVPALVLGLSHGMVAADFFHKQGSVVLLFFGWVTGAQLFFSIHKMPSESPLRTLTMVLLSFFIVVVGYTLIAHTFENFLYPDAELRDAIYAAASINVLAFDTLVVLVTVALVGSWLATFYTHSLEFGGKPGTRWNAIYLNLYALFSREFYVSYIYTQLARGTLSATKRVNIWLRWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRB8</accession>
    <name>Q3SRB8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Arsenate reductase</fullName>
            <ecNumber evidence="3">1.20.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1913</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>[glutaredoxin]-dithiol + arsenate + glutathione + H(+) = arsenite + glutathionyl-S-S-[glutaredoxin] + H2O</text>
            <dbReference type="Rhea" id="RHEA:22016"/>
            <dbReference type="Rhea" id="RHEA-COMP:10729"/>
            <dbReference type="Rhea" id="RHEA-COMP:17668"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29242"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:48597"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:146199"/>
            <dbReference type="EC" id="1.20.4.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 2 3">Belongs to the ArsC family.</text>
    </comment>
    <dbReference type="EC" id="1.20.4.1" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1913"/>
    <dbReference type="EnsemblBacteria" id="ABA05173">
        <property type="protein sequence ID" value="ABA05173"/>
        <property type="gene ID" value="Nwi_1913"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1913"/>
    <dbReference type="eggNOG" id="COG1393">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116644_0_0_5"/>
    <dbReference type="OMA" id="YLKHPPD"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008794">
        <property type="term" value="F:arsenate reductase (glutaredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03034">
        <property type="entry name" value="ArsC_ArsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006659">
        <property type="entry name" value="Arsenate_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006660">
        <property type="entry name" value="Arsenate_reductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30041">
        <property type="entry name" value="PTHR30041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03960">
        <property type="entry name" value="ArsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00014">
        <property type="entry name" value="arsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51353">
        <property type="entry name" value="ARSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA05173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="132" mass="14748" checksum="CEFCFC0132DF1312" modified="2005-10-11" version="1">MPTMTVTIYHNPSCSKSRDTLAMIRQGGEEPVIIEYLKHPPDRSRLRALADAMGLPIRGLLRENGSPYEELDLGNPKWTDDQLLDFILAHPILLQRPIVETPKGVRICRPPERVLDLLTSPAAGEGGENTQR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-03" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRF2</accession>
    <name>NUOI_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADH-quinone oxidoreductase subunit I</fullName>
            <ecNumber evidence="1">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">NADH dehydrogenase I subunit I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">NDH-1 subunit I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nuoI</name>
        <name type="ordered locus">Nwi_1879</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 2 [4Fe-4S] clusters per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 23 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315135.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRF2"/>
    <dbReference type="SMR" id="Q3SRF2"/>
    <dbReference type="STRING" id="323098.Nwi_1879"/>
    <dbReference type="EnsemblBacteria" id="ABA05139">
        <property type="protein sequence ID" value="ABA05139"/>
        <property type="gene ID" value="Nwi_1879"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1879"/>
    <dbReference type="eggNOG" id="COG1143">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067218_5_1_5"/>
    <dbReference type="OMA" id="RGDLYYT"/>
    <dbReference type="OrthoDB" id="1619561at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050136">
        <property type="term" value="F:NADH dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01351">
        <property type="entry name" value="NDH1_NuoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010226">
        <property type="entry name" value="NADH_quinone_OxRdtase_chainI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10849">
        <property type="entry name" value="PTHR10849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01971">
        <property type="entry name" value="NuoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0874">Quinone</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0830">Ubiquinone</keyword>
    <feature type="chain" id="PRO_0000250919" description="NADH-quinone oxidoreductase subunit I">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 1" evidence="1">
        <location>
            <begin position="52"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 2" evidence="1">
        <location>
            <begin position="93"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01351"/>
        </source>
    </evidence>
    <sequence length="162" mass="18673" checksum="822FD9AB0C53776C" modified="2005-10-11" version="1">MSINATARSLLLTEFISAFFLTMRYFFKPKPTINYPFEKNPISPRFRGEHALRRYPNGEERCIACKLCEAVCPAQAITIEAGPRRNDGTRRTVRYDIDMVKCIYCGLCQEACPVDAIVEGPNFEFATETREELYYDKAKLLANGDRWEREIAKAIELDAPYR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRJ0</accession>
    <name>LEXA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">LexA repressor</fullName>
            <ecNumber evidence="1">3.4.21.88</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lexA</name>
        <name type="ordered locus">Nwi_1841</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of Ala-|-Gly bond in repressor LexA.</text>
            <dbReference type="EC" id="3.4.21.88"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S24 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.88" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315097.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRJ0"/>
    <dbReference type="SMR" id="Q3SRJ0"/>
    <dbReference type="STRING" id="323098.Nwi_1841"/>
    <dbReference type="MEROPS" id="S24.001"/>
    <dbReference type="EnsemblBacteria" id="ABA05101">
        <property type="protein sequence ID" value="ABA05101"/>
        <property type="gene ID" value="Nwi_1841"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1841"/>
    <dbReference type="eggNOG" id="COG1974">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066192_45_2_5"/>
    <dbReference type="OMA" id="HVWLLPH"/>
    <dbReference type="OrthoDB" id="1933795at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06529">
        <property type="entry name" value="S24_LexA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00015">
        <property type="entry name" value="LexA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006200">
        <property type="entry name" value="LexA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039418">
        <property type="entry name" value="LexA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006199">
        <property type="entry name" value="LexA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006197">
        <property type="entry name" value="Peptidase_S24_LexA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015927">
        <property type="entry name" value="Peptidase_S24_S26A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01726">
        <property type="entry name" value="LexA_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00717">
        <property type="entry name" value="Peptidase_S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00726">
        <property type="entry name" value="LEXASERPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="SSF51306"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00498">
        <property type="entry name" value="lexA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0678">Repressor</keyword>
    <keyword id="KW-0742">SOS response</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_1000001309" description="LexA repressor">
        <location>
            <begin position="1"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="active site" description="For autocatalytic cleavage activity" evidence="1">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <feature type="active site" description="For autocatalytic cleavage activity" evidence="1">
        <location>
            <position position="192"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="1">
        <location>
            <begin position="119"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00015"/>
        </source>
    </evidence>
    <sequence length="233" mass="25403" checksum="D82188147F95AEB2" modified="2005-10-11" version="1">MLTRKQYELLRFINERLKESGVPPSFDEMKDALDLRSKSGIHRLITALEERGFIRRLPNRARAIEVIKLPELAASGGGRRGFTPSVIEGNLGKVRRGGGGGADEAERPVAVPVMGRIAAGTPIEALQTCSHTISLPPDMLGAGEHYALEVRGDSMVDAGILDGDMALIQRNPTADTGDIVVALIDDEEATLKRFRRRGASIALEPANAAYEVRILPPNRVQIQGRLVGIYRKY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRN1</accession>
    <name>COAD_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphopantetheine adenylyltransferase</fullName>
            <ecNumber evidence="1">2.7.7.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Dephospho-CoA pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Pantetheine-phosphate adenylyltransferase</fullName>
            <shortName evidence="1">PPAT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">coaD</name>
        <name type="ordered locus">Nwi_1799</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Reversibly transfers an adenylyl group from ATP to 4'-phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-4'-phosphopantetheine + ATP + H(+) = 3'-dephospho-CoA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19801"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="2.7.7.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 4/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial CoaD family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.3" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA05060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011315056.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRN1"/>
    <dbReference type="SMR" id="Q3SRN1"/>
    <dbReference type="STRING" id="323098.Nwi_1799"/>
    <dbReference type="EnsemblBacteria" id="ABA05060">
        <property type="protein sequence ID" value="ABA05060"/>
        <property type="gene ID" value="Nwi_1799"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1799"/>
    <dbReference type="eggNOG" id="COG0669">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100149_0_1_5"/>
    <dbReference type="OMA" id="EFQMALM"/>
    <dbReference type="OrthoDB" id="1846503at2"/>
    <dbReference type="UniPathway" id="UPA00241">
        <property type="reaction ID" value="UER00355"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004595">
        <property type="term" value="F:pantetheine-phosphate adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02163">
        <property type="entry name" value="PPAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00151">
        <property type="entry name" value="PPAT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001980">
        <property type="entry name" value="PPAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21342:SF1">
        <property type="entry name" value="PTHR21342:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01020">
        <property type="entry name" value="LPSBIOSNTHSS"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01510">
        <property type="entry name" value="coaD_prev_kdtB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000011190" description="Phosphopantetheine adenylyltransferase">
        <location>
            <begin position="1"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="94"/>
            <end position="96"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="129"/>
            <end position="135"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00151"/>
        </source>
    </evidence>
    <sequence length="165" mass="17486" checksum="020A9B0ACCA7F9BE" modified="2005-10-11" version="1">MPRVALYPGSFDPVTNGHLDVVRHAVALCDRLIVAVGVHPGKTPVFSADDRLAMARSVFEPVAVEAGCAFDCVTYDDLTVAAAHKVGATILVRGLRDGTDLDYEMQIAGMNETMAPDVHTVFVPASPGVRPITATLVRQIATMGGDVSPFVPQLVASRLRTKFAG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SRY6</accession>
    <name>RL9_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplI</name>
        <name type="ordered locus">Nwi_1694</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314953.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SRY6"/>
    <dbReference type="SMR" id="Q3SRY6"/>
    <dbReference type="STRING" id="323098.Nwi_1694"/>
    <dbReference type="EnsemblBacteria" id="ABA04955">
        <property type="protein sequence ID" value="ABA04955"/>
        <property type="gene ID" value="Nwi_1694"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1694"/>
    <dbReference type="eggNOG" id="COG0359">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078938_1_0_5"/>
    <dbReference type="OMA" id="MKIILTH"/>
    <dbReference type="OrthoDB" id="1959318at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.430.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00503">
        <property type="entry name" value="Ribosomal_L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000244">
        <property type="entry name" value="Ribosomal_L9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009027">
        <property type="entry name" value="Ribosomal_L9/RNase_H1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020594">
        <property type="entry name" value="Ribosomal_L9_bac/chp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020069">
        <property type="entry name" value="Ribosomal_L9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036791">
        <property type="entry name" value="Ribosomal_L9_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020070">
        <property type="entry name" value="Ribosomal_L9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036935">
        <property type="entry name" value="Ribosomal_L9_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21368">
        <property type="entry name" value="PTHR21368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03948">
        <property type="entry name" value="Ribosomal_L9_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01281">
        <property type="entry name" value="Ribosomal_L9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55653">
        <property type="entry name" value="SSF55653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55658">
        <property type="entry name" value="SSF55658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00158">
        <property type="entry name" value="L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00651">
        <property type="entry name" value="RIBOSOMAL_L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000236550" description="50S ribosomal protein L9">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="176"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="188"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="202" mass="21973" checksum="F97DE516C399DEA9" modified="2005-10-11" version="1">MEIILLERVAKLGQMGEVVKVKDGFARNFLLRRGKALRATAENRAKYDGMKAELEANNIKAKGEAATVAEKINGRDIVVIRQASETGQLFGSVTVRDIVAALAADGIIVSRPQVWLDAPIKTIGQQKLTVAVHPEVEAEITVTVARSVDEAERIQRGEDISTRREDRDAAAEAIAAAGEFFDPDAQHDDEPAAEDDQNAEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SS65</accession>
    <name>Q3SS65_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_1615</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314880.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1615"/>
    <dbReference type="EnsemblBacteria" id="ABA04876">
        <property type="protein sequence ID" value="ABA04876"/>
        <property type="gene ID" value="Nwi_1615"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1615"/>
    <dbReference type="eggNOG" id="COG1511">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5281">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008236_0_0_5"/>
    <dbReference type="OMA" id="QNIYITI"/>
    <dbReference type="OrthoDB" id="1006982at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006431">
        <property type="entry name" value="Phage_tape_meas_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013491">
        <property type="entry name" value="Tape_meas_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09718">
        <property type="entry name" value="Tape_meas_lam_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20155">
        <property type="entry name" value="TMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02675">
        <property type="entry name" value="tape_meas_nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TMP_3" evidence="2">
        <location>
            <begin position="90"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Tape_meas_lam_C" evidence="1">
        <location>
            <begin position="485"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA04876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="671" mass="69525" checksum="DF4609BA595F19F0" modified="2005-10-11" version="1">MAEETGERLVVMLEARISEFEKRMVKAEQTGTRTYRQLTAGSSRATKNMEQDMLRSTVAINRSLAQTSVQIGSFAKAFAVGIAGSAAFRSVSKAAQEFTSLQNALKVTGLEGAALDKVFSSLFQIAQKNGTAIEPLVTLYSRASQAQKELKANSADLMKFTDGVSIALRVAGTNSTQAAGALLQLSQAIGSGVVRAEEFNSVNEGARPILQAVAAGLKEAGGSVSALKTLVNDGKVSSEAFFRAFLAGMPQIEAQAAKADGTIGQASARIGNAFIALVGHLDKTTGASGNAAQGLNAVAGVVEKLGGYFDAASGKLETLQKWLTSVGNNSAWTTIAKFFGADFSPEEMAKYGLTPVGATPATPARVVVTGGTTEAPKVNPVSLTDFKVPGKAGSGAAATRDPFERSNDQIAKRIELLKVEAATIDQTAAEQDRARVVVEMETAAKQANERAGKKNVEVTGEQRAKIEALADAYAKARGELERLNSPLASFARESADVGQQLERLAVGSLDRIADDFGEIVTGTKTVEEAFKSMARSILAELAKIAVKQAVLGPLANLMGGGGSGGGILSALIPKFANGTDNAPGGLAIVGERGPELVNLPRGSQVIPHHQIGSAGGGGDINVPVMVNIDATGADAAGLARVQRQVADLKANLPGLAVQAIQSAKNRRVRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSL2</accession>
    <name>Q3SSL2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1468</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04729">
        <property type="protein sequence ID" value="ABA04729"/>
        <property type="gene ID" value="Nwi_1468"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1468"/>
    <dbReference type="HOGENOM" id="CLU_1480885_0_0_5"/>
    <dbReference type="OMA" id="ERTIIKV"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="195" mass="22673" checksum="2FE65111865A2914" modified="2005-10-11" version="1">MSSRKLLRRGYRMRHMDYFEQAEEETRRFLNRPDMQFVVRAKQYLYAASILVQSERTIIKVPTLHLIAHGIEVLLKAPLVRASGDVEEVRKEFRHDIQKLWLADCNAEIRAKVFRIASLTWEEARASGKYRDAFAEKPDEAIECSLRDLSRLHSPETKFALKYIIPEGEIGPRAPHLVETFLEVADECLKNPSSF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSP1</accession>
    <name>LIPA_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lipoyl synthase</fullName>
            <ecNumber evidence="1">2.8.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Lip-syn</fullName>
            <shortName evidence="1">LS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Lipoate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Lipoic acid synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Sulfur insertion protein LipA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lipA</name>
        <name type="ordered locus">Nwi_1439</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[[Fe-S] cluster scaffold protein carrying a second [4Fe-4S](2+) cluster] + 4 H(+) + N(6)-octanoyl-L-lysyl-[protein] + 2 oxidized [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = (R)-N(6)-dihydrolipoyl-L-lysyl-[protein] + 2 5'-deoxyadenosine + [[Fe-S] cluster scaffold protein] + 4 Fe(3+) + 2 hydrogen sulfide + 2 L-methionine + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:16585"/>
            <dbReference type="Rhea" id="RHEA-COMP:9928"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:14568"/>
            <dbReference type="Rhea" id="RHEA-COMP:14569"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:33722"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:78809"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="EC" id="2.8.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the radical SAM superfamily. Lipoyl synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.8" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314708.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SSP1"/>
    <dbReference type="SMR" id="Q3SSP1"/>
    <dbReference type="STRING" id="323098.Nwi_1439"/>
    <dbReference type="EnsemblBacteria" id="ABA04700">
        <property type="protein sequence ID" value="ABA04700"/>
        <property type="gene ID" value="Nwi_1439"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1439"/>
    <dbReference type="eggNOG" id="COG0320">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033144_2_1_5"/>
    <dbReference type="OMA" id="PYCDIDF"/>
    <dbReference type="OrthoDB" id="1184806at2"/>
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        <property type="reaction ID" value="UER00593"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:lipoate synthase activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:lipoyl synthase activity (acting on glycine-cleavage complex H protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102553">
        <property type="term" value="F:lipoyl synthase activity (acting on pyruvate dehydrogenase E2 protein)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00206">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003698">
        <property type="entry name" value="Lipoyl_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10949">
        <property type="entry name" value="PTHR10949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005963">
        <property type="entry name" value="Lipoyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00271">
        <property type="entry name" value="lipoyl_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00510">
        <property type="entry name" value="lipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000012247" description="Lipoyl synthase">
        <location>
            <begin position="1"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="73"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="5"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="319" mass="35509" checksum="6F5AE3AAE43CC433" modified="2005-10-11" version="1">MVVILDTISANPVRPRHPEKANRPDALSPPKPDWIRVRAPNTRGYANTRRIVKENGLVTVCEEAGCPNIGECWDKKHATFMIMGDTCTRACAFCNVKTGMPGAIESSEPEYVAEATRKLGLAHVVVTSVDRDDLDDGGAEHFAQTIRAIRERCPATTIEILTPDFLRKDGALEKVVAAKPDVFNHNLETVPSRYLTVRPGARYFHSIRLLQRVKEIDPAIFTKSGIMVGLGEERHEVLQVMDDLRSADVDFLTIGQYLQPTRKHHAVIRYVTPEEFSSYETVAYTKGFLMVSASPLTRSSHHAGEDFAKLQAARATLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSP8</accession>
    <name>Q3SSP8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Response regulator receiver modulated diguanylate cyclase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_1432</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314702.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1432"/>
    <dbReference type="EnsemblBacteria" id="ABA04693">
        <property type="protein sequence ID" value="ABA04693"/>
        <property type="gene ID" value="Nwi_1432"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1432"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_11_28_5"/>
    <dbReference type="OMA" id="NDYFVYP"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="4"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="157"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="3">
        <location>
            <begin position="322"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA04693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="457" mass="50645" checksum="B2E13C92741A5EE9" modified="2005-10-11" version="1">MSARVLVVDDVPANVRLLEARLSAEYFDVLTASNGAQALETCARAECDIILLDVMMPDMDGFEVCRRLKANPATHFIPVVMITALDNPSDRVRGLEAGADDFLTKPVSDVVLTARVRSLTRLKMMTDELRLRAMTSLEIGMQAPEREAVCDSGKGGRILLVDDRPSSYERLASMLSAEHAVDVEADPSEALFRAAESNYDLLIVSLGLENFDGLRLCSQARSLERTRHVPILAVADADGSPRLLRGLEIGINDYLLRPIDRNELLARARTQIRRRRYTVHLRDNVASSIEMAVTDGLTGLHNRRYMESHLTTLAEQASLRGQSLALMMLDIDYFKAINDNYGHDAGDDVLREFALRIRKAIRGIDLACRYGGEEFVIVMPETDLHVAGMVAERLRRAIAGEPFSIHKGTKLIKVTISIGLSTLERKGEPISDVVKRADVALYRAKHDGRNRVVAEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSQ2</accession>
    <name>Q3SSQ2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1428</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314698.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1428"/>
    <dbReference type="EnsemblBacteria" id="ABA04689">
        <property type="protein sequence ID" value="ABA04689"/>
        <property type="gene ID" value="Nwi_1428"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1428"/>
    <dbReference type="eggNOG" id="ENOG502ZXPR">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064490_0_0_5"/>
    <dbReference type="OMA" id="FRFVTQI"/>
    <dbReference type="OrthoDB" id="1176608at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015000">
        <property type="entry name" value="EipB-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08904">
        <property type="entry name" value="EipB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229256" evidence="1">
        <location>
            <begin position="36"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="295" mass="32067" checksum="78C3E1D4788C4083" modified="2005-10-11" version="1">MRHYSRCSASRLLPWFLTTIVAAASLPALSGAAYAAAGVSFLSHQALYNLSLVKSRGSSSINGARGRILYKFSGSACEGYTSDFRQVSEMDNGEGETTLSDMRSTSWEDAEGKAYRFKIVTRANDSDQSEVDGIAERKGGKITVKLKRPVAKTVTIDGKAVFPTEQIQRIIAAAKAGKSVLELTVYDGSDNGEKVYNTFSVIGKPIRGEATGSTPDPSTANDQMKTLTRWPVTVSYYERGAPHTSGEQTPNYSISFELFEDGVSRALVLDYNDFVISGKMDKFDLSDVRSAKPCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SSZ8</accession>
    <name>Q3SSZ8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1332</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314611.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1332"/>
    <dbReference type="EnsemblBacteria" id="ABA04593">
        <property type="protein sequence ID" value="ABA04593"/>
        <property type="gene ID" value="Nwi_1332"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1332"/>
    <dbReference type="eggNOG" id="COG5330">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035493_0_0_5"/>
    <dbReference type="OMA" id="PFSWDRI"/>
    <dbReference type="OrthoDB" id="806188at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019285">
        <property type="entry name" value="DUF2336"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10098">
        <property type="entry name" value="DUF2336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="516"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="556" mass="60291" checksum="26CFAE3D710FB045" modified="2005-10-11" version="1">MIIRQFLSWVRTASAGARAEATRALARAWLISDLSDDERAAAEGGLLLQLDDSSPLVRRAMADVFARSANAPPTIVRALSVDQPSVAIPVLEHSPLLTDADLVDIVATGSCEVQCAVARRVRVPVPVCAAIAEVGSAAAALELIENPGADLARVSWDRIVERHGHLPAIREAMLALDGLPAVTRLLLAVKLSDSLARFVVARDWLSAERAGRTVSDARDRSIVDIVARAREDEMTCLVAHLRATGQLTAGLMLRALLSGTLGLFHHALADLSGLPFSRVTALVHDRGGASLPALLTRAGLPESAFPAFRAALEAHEEIGFVATVDGAVRLRRRMVEHVLTVCEAASAEISQPLLTLLRRFATESARDEARVFCDELVAEAARSPMIEDVPAGETALRDLEVREEFYEPVPAIALPSEVEINSLEEDASDQNAGVVYRFGEGMKTSRPRFLDRLALKTRFAEYEPRDAELDDATSPDAVPYEALSCEVLPYETLSYEDEPVEDGPYIVSSCEPKRVEPRFDEPAPYDSLYREPDFSGLKNAPPAYREPLLSDLLAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3ST81</accession>
    <name>Q3ST81_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF87 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1248</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1248"/>
    <dbReference type="EnsemblBacteria" id="ABA04510">
        <property type="protein sequence ID" value="ABA04510"/>
        <property type="gene ID" value="Nwi_1248"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1248"/>
    <dbReference type="eggNOG" id="COG0433">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023842_0_0_5"/>
    <dbReference type="OMA" id="CLVSQRP"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008571">
        <property type="entry name" value="HerA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002789">
        <property type="entry name" value="HerA_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957">
        <property type="entry name" value="PTHR42957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01935">
        <property type="entry name" value="DUF87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF87" evidence="1">
        <location>
            <begin position="160"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="617" mass="68460" checksum="0AA6F37E27B7493C" modified="2005-10-11" version="1">MPPLRGRLNRFLPQPCKHWRRSSALDTRNVVRPMLHSISQNSFGRVISVRGSQARVGILRTGRMSMAAARATVGRFVSLRSMTSTIVAMITEVSCEHLPSSDEFMASASVDLLGEILDSSGPRPRFQRGVTNYPAIDDLADLVTNQELRTIYAPNGADQIDVGTLQQDPSVTAFIDIEEMLSKHFAVLGSTGVGKSSGVSLLINEILQSRPDLRIFLLDVHNEYGRCFGDRALVLNPRNLKLPFWLFNFEEIVDVLFGGRPGVPEELDILAEVIPIAKGIYTQYQHADRVSLKRTDPKSVGFTSDTPVPYRLVDMISLIDERMGRLENRSSRIVYHKLISRIETVRNDPRYAFMFDNANVGGDTMAEVVSLLFRLPANGRPMTIMQLAGFPAEVVDSVVSVLCRMAFDFGLWSDGVSPLLFVCEEAHRYASADRNIGFGPTRKAISRIAKEGRKYGVYLGLVTQRPAELDATIISQCNTLFTMRLANDRDQSLLRSAVSDAAANLLSFVPSLGTREVLAFGEGVALPTRLRFKEVPAHQLPRSEAKISTVPSSSHGQDMVFVDAVLERWRGATSHRDAPHTQAPMERPTLSVLDAQMLQPSLGLDPDRFTLLKRPLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STF7</accession>
    <name>Q3STF7_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1172</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314463.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1172"/>
    <dbReference type="EnsemblBacteria" id="ABA04434">
        <property type="protein sequence ID" value="ABA04434"/>
        <property type="gene ID" value="Nwi_1172"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1172"/>
    <dbReference type="eggNOG" id="ENOG5032SBG">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085951_0_1_5"/>
    <dbReference type="OMA" id="ADWFENR"/>
    <dbReference type="OrthoDB" id="1829390at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd08054">
        <property type="entry name" value="gp6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021146">
        <property type="entry name" value="Phafe_gp6_head-tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011738">
        <property type="entry name" value="Phage_CHP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05135">
        <property type="entry name" value="Phage_connect_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02215">
        <property type="entry name" value="phage_chp_gp8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="187" mass="19964" checksum="FFA451DB991CE4AE" modified="2005-10-11" version="1">MPSILLTGPAVEPWSVEEAKSFLRAENDDDDAVIASLIAAARSHVEAMTRCALIAQTWRFVLDQWPKDGRIRPGRGPLRTLAAARVYNSAGIATPIDAGTFVLDKAAGVIASSVWVFPGRASGGIELDIEIGFGDAASDVPDVLRHAVRTLVTHWYENRGLVAIGQSVAMMPASVSAMIASYRVHAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STI2</accession>
    <name>Q3STI2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_1147</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1147"/>
    <dbReference type="EnsemblBacteria" id="ABA04409">
        <property type="protein sequence ID" value="ABA04409"/>
        <property type="gene ID" value="Nwi_1147"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1147"/>
    <dbReference type="eggNOG" id="ENOG5032SY5">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1198763_0_0_5"/>
    <dbReference type="OMA" id="DEPHRSH"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="281" mass="31336" checksum="8E6B13054C4A84F7" modified="2005-10-11" version="1">MQKTPLATLGLGRSRLASLLSVILLGTALAACSGGDFGRVRQDARNDDMHRWMGAEATGSIGRRPSRFQLTDNERQLRDLAYPLVEPPHSRPAWKSVFGNYDPLAAPWRQKPAFDRSAYGRLLLDEPHRSHASRYAQLIDDVRDDITRFDPFFNTARQVVDLDSKRNASLRLVSDLSPRERADAVARMEENTLVVQWVQQCLEQRIASYRWALEHLVIHAPDGVAADADRLIGHLAALTASPPVASAPVIGRALRTRGSAHRVFERSLSSARAPRMDDLFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STQ0</accession>
    <name>Q3STQ0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Xanthine dehydrogenase, molybdenum binding subunit apoprotein</fullName>
            <ecNumber evidence="2">1.17.1.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_1079</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.17.1.4" evidence="2"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314375.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1079"/>
    <dbReference type="EnsemblBacteria" id="ABA04341">
        <property type="protein sequence ID" value="ABA04341"/>
        <property type="gene ID" value="Nwi_1079"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1079"/>
    <dbReference type="eggNOG" id="COG1529">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001681_2_0_5"/>
    <dbReference type="OMA" id="IGSCPAV"/>
    <dbReference type="OrthoDB" id="117507at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004854">
        <property type="term" value="F:xanthine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000674">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036856">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016208">
        <property type="entry name" value="Ald_Oxase/xanthine_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008274">
        <property type="entry name" value="AldOxase/xan_DH_Mopterin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037165">
        <property type="entry name" value="AldOxase/xan_DH_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908">
        <property type="entry name" value="PTHR11908"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01315">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02738">
        <property type="entry name" value="Ald_Xan_dh_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01008">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54665">
        <property type="entry name" value="SSF54665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56003">
        <property type="entry name" value="SSF56003"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ald_Xan_dh_C" evidence="1">
        <location>
            <begin position="22"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="763" mass="81556" checksum="B30872280206B50C" modified="2005-10-11" version="1">MAPVKFGIGQSVRRKEDDAFIRGKGRYTDDHAPPDALHALMLRSPHAHAAYRIDVSRACRLPGVALILTATDVADLGGLPCQVELPDQPFIAPPYPILPKDEVRHVGDAIAFVVADTVERARDALEAIEVEWTPLASVVGVADAVRDGAPQVWPDHAGNVLFDVSLGDRKAADAAFAAADAVAEISIVNPRVVTNYMETRAAVCVYDARRDHLTLTVGSQGSHRLREILCENVLGIPLEKMRVICPDVGGGFGTKLFPYREYALIAVAAKKLHTAIRWTAERSDHFVGDSQGRDNVTTARMALAGDGRFLGMDVDLMGDMGAYLSTFGPDIPYSGAGMLPGLYDIQAFHCRVRAVFTNTVPVDAYRGAGRPEAAYVVERLVDAAARKIGMTPDAIRRKNFIPPEAMPYRTATGKIYDSGDFAAHMARAMEIAKWREFAGRAEAAKERGLVRGIGLATYVEVCGTMGRERADVRLDANGDVTVLIGTQSTGQGHATAYAQIIADQFGIPPERVHMKQGDTDVVTTGLGTGGSSSIPSGGVSVERATRKLGVRLREIASEALETSAADLEISDGSIRIAGTDRFVSFADLAKRADLSKLTADESFSSADGTYPNGTHVVEVEIDPATGKIELVNYVVVDDFGVTLNPLLLAGQVHGGAMQGIGQALMEQTVYDADGQLVTGTLMDYAVPRAADGVSITFETRNVPCATNPLGIKGAGEAGAIGSCPAVVNAIIDGLWREYGIDHIDMPATPERVWTAIRDAQRRL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-16" modified="2022-05-25" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STT1</accession>
    <name>MRAY_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phospho-N-acetylmuramoyl-pentapeptide-transferase</fullName>
            <ecNumber evidence="1">2.7.8.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">UDP-MurNAc-pentapeptide phosphotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mraY</name>
        <name type="ordered locus">Nwi_1048</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the initial step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan: transfers peptidoglycan precursor phospho-MurNAc-pentapeptide from UDP-MurNAc-pentapeptide onto the lipid carrier undecaprenyl phosphate, yielding undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide, known as lipid I.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>di-trans,octa-cis-undecaprenyl phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine = di-trans-octa-cis-undecaprenyl diphospho-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine + UMP</text>
            <dbReference type="Rhea" id="RHEA:28386"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:60392"/>
            <dbReference type="ChEBI" id="CHEBI:61386"/>
            <dbReference type="ChEBI" id="CHEBI:61387"/>
            <dbReference type="EC" id="2.7.8.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 4 family. MraY subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.13" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314344.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3STT1"/>
    <dbReference type="SMR" id="Q3STT1"/>
    <dbReference type="STRING" id="323098.Nwi_1048"/>
    <dbReference type="EnsemblBacteria" id="ABA04310">
        <property type="protein sequence ID" value="ABA04310"/>
        <property type="gene ID" value="Nwi_1048"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1048"/>
    <dbReference type="eggNOG" id="COG0472">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023982_0_0_5"/>
    <dbReference type="OMA" id="LMSPLHH"/>
    <dbReference type="OrthoDB" id="1151822at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008963">
        <property type="term" value="F:phospho-N-acetylmuramoyl-pentapeptide-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051992">
        <property type="term" value="F:UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine:undecaprenyl-phosphate transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06852">
        <property type="entry name" value="GT_MraY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00038">
        <property type="entry name" value="MraY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000715">
        <property type="entry name" value="Glycosyl_transferase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003524">
        <property type="entry name" value="PNAcMuramoyl-5peptid_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018480">
        <property type="entry name" value="PNAcMuramoyl-5peptid_Trfase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22926">
        <property type="entry name" value="PTHR22926"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00953">
        <property type="entry name" value="Glycos_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10555">
        <property type="entry name" value="MraY_sig1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00445">
        <property type="entry name" value="mraY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01347">
        <property type="entry name" value="MRAY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01348">
        <property type="entry name" value="MRAY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0133">Cell shape</keyword>
    <keyword id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000235459" description="Phospho-N-acetylmuramoyl-pentapeptide-transferase">
        <location>
            <begin position="1"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="296"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="345"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00038"/>
        </source>
    </evidence>
    <sequence length="368" mass="39451" checksum="7731CCF73312F0DF" modified="2005-10-11" version="1">MFYWLIDLSNTVPGLGIFKPLLNVFRYITFRTGGAVVTGALFVFLFGPWIIDNLRLRQGKGQPIRADGPQSHLVSKKGTPTMGGLMILSGLVVSTVLWANPLNPYVWIVLAVTLGFGLIGFYDDYLKVTKQTHAGFSGRTRLLLELLIALAACYALTRLGREPFSTALVIPFFKDVALDLGWFFLGFGAFIIVGAGNAVNLTDGLDGLAIVPVMIAAASFAMIAYLAGNAVFADYLQINYIAGAGELAVLCGAVLGAGLGFLWFNAPPASIFMGDTGSLALGGMLGSIAVAVKHEIVLAVIGGLFVLEAVSVIVQVASFKLTGKRIFKMAPIHHHFEQLGWTEPQIVIRFWIISVMLALAGLSTLKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STV1</accession>
    <name>Q3STV1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two component transcriptional regulator, LuxR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_1028</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314324.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_1028"/>
    <dbReference type="EnsemblBacteria" id="ABA04290">
        <property type="protein sequence ID" value="ABA04290"/>
        <property type="gene ID" value="Nwi_1028"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_1028"/>
    <dbReference type="eggNOG" id="COG4566">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_4_5"/>
    <dbReference type="OMA" id="KMNKHIA"/>
    <dbReference type="OrthoDB" id="930905at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="6"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="136"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="205" mass="22420" checksum="A5EBBD50EED1B04A" modified="2005-10-11" version="1">MSHKGNVYVIDDDPTIRDSLDFLLGSANFDVTLFDRAADFLDSHSGLGFGCVVSDVRMPGIDGMDLLRRLKAHASPLPVIIMTGHGDIPLAVEAMKLGAIDFVEKPFEDERLIGMIEAALEQGPDQTKGDSISAEIVSRIATLSQRERQVMDGLVAGLSNKLIAREHNISPRTIEVYRANVMTKMQAHNLSELIRLALRAGILRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3STZ8</accession>
    <name>Q3STZ8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0981</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314282.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04243">
        <property type="protein sequence ID" value="ABA04243"/>
        <property type="gene ID" value="Nwi_0981"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0981"/>
    <dbReference type="eggNOG" id="ENOG5030YYN">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145244_4_1_5"/>
    <dbReference type="OMA" id="QRMACTP"/>
    <dbReference type="OrthoDB" id="2024195at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="94" mass="10040" checksum="D3560D9499986DF8" modified="2005-10-11" version="1">MLRHGVVLFSGILAGACIVSTAYAEEYRGTFAQRMACTPDVFRLCRSEIPDVGRIVACLRKNQPRLGGPCRAVFEANADAGGDDVVAKATGSHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUA1</accession>
    <name>Q3SUA1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Penicillin amidase, Cysteine peptidase, MEROPS family C59</fullName>
            <ecNumber evidence="5">3.5.1.11</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Nwi_0876</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C59 family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.11" evidence="5"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314181.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04140">
        <property type="protein sequence ID" value="ABA04140"/>
        <property type="gene ID" value="Nwi_0876"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0876"/>
    <dbReference type="eggNOG" id="COG3049">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045206_0_1_5"/>
    <dbReference type="OMA" id="KQVIHHG"/>
    <dbReference type="OrthoDB" id="636485at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008953">
        <property type="term" value="F:penicillin amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029132">
        <property type="entry name" value="CBAH/NAAA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02275">
        <property type="entry name" value="CBAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229217" evidence="3">
        <location>
            <begin position="29"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="CBAH" evidence="4">
        <location>
            <begin position="29"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABA04140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="354" mass="39329" checksum="35A719E9290393BC" modified="2005-10-11" version="1">MSQKYSRASRCAALFVSVNLLFTPVAEACTRFVYHGADGEVITARSMDWTVDIGTNLWVFPRGMKRSGEAGPNSIRWTSKYGSVIASGYDISTTDGMNEAGLVTNVLWLVESSYPEYDGKSPTLSIAAWAQYMLDNFATVREAVDTLAKESFTIVTNKVPGQDRLATLHLSLSDATGDSAIIEFIGGKQIIHHGRDFQVMTNSPIFEKQLALNEYWTQIGGTVMLPGTNRASDRFTRASFYVNAIPKTEKPVEAIASVFSVIRNVSVPFGITTPDQPNISSTRWRTVSDQKRRLYFFESALTPNIFWVDLTKLDFSEKTGKVLKLDLGPDQTHIYSGVANDQFKEAKPFKFLGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUE0</accession>
    <name>Q3SUE0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0837</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314149.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04101">
        <property type="protein sequence ID" value="ABA04101"/>
        <property type="gene ID" value="Nwi_0837"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0837"/>
    <dbReference type="eggNOG" id="COG4453">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152494_1_2_5"/>
    <dbReference type="OMA" id="WIGEPAK"/>
    <dbReference type="OrthoDB" id="2011686at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014795">
        <property type="entry name" value="Vibrio_phage_ICP1_Orf50"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08681">
        <property type="entry name" value="DUF1778"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="SSF47598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA04101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="88" mass="9997" checksum="C6521F8612CA2B35" modified="2005-10-11" version="1">MPNLAPVSVRVSKKERELLEAAADQSRTNLSDFIRRKAVEAAEIDLLQQTRVTIPAAKWKQFEAWVNSPAKEIPALKKLSKSRPVWQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUE9</accession>
    <name>Q3SUE9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0827</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314140.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0827"/>
    <dbReference type="EnsemblBacteria" id="ABA04092">
        <property type="protein sequence ID" value="ABA04092"/>
        <property type="gene ID" value="Nwi_0827"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0827"/>
    <dbReference type="eggNOG" id="ENOG5030WUD">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1376937_0_0_5"/>
    <dbReference type="OMA" id="WEWIARP"/>
    <dbReference type="OrthoDB" id="1429522at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="211" mass="23191" checksum="AE04E49FB1394379" modified="2005-10-11" version="1">MPAPPRSFLTVTDTAIRWQVPALDIAGWAIDGEIALSAVLPTVETADKQIASGIVEIDGADVVALFRRDGASRKPALVRRFRRSKKTEWEWIARPVDGVAVTSADVIILRKEFERCERAWGLFDAGAAESGPKASTQAKRAAGPGAPPKHDWDAFAGAIARRVHDHGMPVSQGELVRDMMDWFAGREDFPPPDERTVRRKVSAIWKELASQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUJ5</accession>
    <name>Q3SUJ5_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase (Class I)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0781</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA04046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABA04046">
        <property type="protein sequence ID" value="ABA04046"/>
        <property type="gene ID" value="Nwi_0781"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0781"/>
    <dbReference type="eggNOG" id="COG2801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2035577_0_0_5"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA04046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="121" mass="13632" checksum="145F60B94E82ACDD" modified="2005-10-11" version="1">MDEARGSIADLPTRSKEPHSSSLSDRAEATIVAFRKYTLPCCRSTTASMRFSRRSRIRLARHCIAVYSVVAYPDCSNVECDKAPGRKFERYPIGYVHFNNSQTLPIPGPRSTNCNGFFDDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUT1</accession>
    <name>Q3SUT1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cytochrome c Hsc</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_0694</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011314016.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0694"/>
    <dbReference type="EnsemblBacteria" id="ABA03960">
        <property type="protein sequence ID" value="ABA03960"/>
        <property type="gene ID" value="Nwi_0694"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0694"/>
    <dbReference type="eggNOG" id="COG1858">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041010_0_0_5"/>
    <dbReference type="OMA" id="DCHANGH"/>
    <dbReference type="OrthoDB" id="52724at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004229234" evidence="6">
        <location>
            <begin position="23"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="7">
        <location>
            <begin position="357"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="25"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA03960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="466" mass="52275" checksum="F19A98DBF0C968CD" modified="2005-10-11" version="1">MPMKTAIGLTTVIAAALASAFAATSDPLPPESTYRPLPTQPFSKVKEDDEAQKPAVMERQRTLMEQRYDLANRPVAGVMMSGGRKAVQQGVRVKLPQGVTWDSLAAKEPNEIRDKDLLPKGFMPLPHVKQATGGQVFPDDQIRKIAHDEQRDLKRFDIDFDLPGHLTPEFPPPIFLTTHPELGDVSRGDLLTIRNFYEYLNGIITPVQMEGLRLLLTPFPQEEFNQTEDRKVAQPSLGVTCLDCHANFHTNAAFHLTPDIRPQAERFRLDTTSLRGMYNQQIHGSKRSLRSIEDFTQFEQHTAYFNGDHVSAARKGMHLPDRANQVAMMAQMQNIIDFPPAPKLDPSGRLDPKKATEQELRGEQVFLGKGRCAECHIPAMSFTDNNMHDLKLERFYKAGQTINGMVAIPDGPIKTFTLRGIKDSPPYLHDGRLLTLADTVEFFNLVLGTKLNQQDKDALVAYLLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUV8</accession>
    <name>Q3SUV8_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Amino acid/amide ABC transporter substrate-binding protein, HAAT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0667</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313989.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0667"/>
    <dbReference type="EnsemblBacteria" id="ABA03933">
        <property type="protein sequence ID" value="ABA03933"/>
        <property type="gene ID" value="Nwi_0667"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0667"/>
    <dbReference type="eggNOG" id="COG0683">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027128_1_2_5"/>
    <dbReference type="OMA" id="PDAVACF"/>
    <dbReference type="OrthoDB" id="538961at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028081">
        <property type="entry name" value="Leu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13458">
        <property type="entry name" value="Peripla_BP_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peripla_BP_6" evidence="1">
        <location>
            <begin position="33"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="423" mass="45783" checksum="F1949A3D0AA23C66" modified="2005-10-11" version="1">MAINPTRRQVLQGAAAIAGSAVFAPAIHADPAPIRLGTLASLEGPLAINGQDAYRVVELLLEEAGYKAGGRKIEWIRESSNGKPDVALARTRKLIEQDKADVVIGPLAGAEGIAVRDYSRTVPNKTFINGSSASQDNTLRNSSSNYFRFSTDGTQWTAGLGEYTKNVLNIGEVTVVASDYAFPYSQVFGFSLEFTRAGGKLNYLWTPFGTSDYTSLIAQIPKSSGALYVVYGGSDGLAFLTQYAQAGGSLPLLGGTLLADQTLFSARGPHRKVLEGTISAGPIGDHYDNPVWKDFQARYVRRWSKDVGQQTPGTLAFAYSTNLQATLLALNAVDGDLSGNQEAFREALRKLEFKNYIDAHVKLDHNRQAISDNFLTKVEVQDKRLQSVVIKKVPNVNQTLGFPEDKYVALGAPSRNNTGNVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUZ3</accession>
    <name>Q3SUZ3_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">23S rRNA m(5)U-1939 methyltransferase</fullName>
            <ecNumber evidence="6">2.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Nwi_0631</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313958.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0631"/>
    <dbReference type="EnsemblBacteria" id="ABA03898">
        <property type="protein sequence ID" value="ABA03898"/>
        <property type="gene ID" value="Nwi_0631"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0631"/>
    <dbReference type="eggNOG" id="COG2265">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014689_8_0_5"/>
    <dbReference type="OMA" id="FYAGDMK"/>
    <dbReference type="OrthoDB" id="1421660at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030390">
        <property type="entry name" value="MeTrfase_TrmA_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010280">
        <property type="entry name" value="U5_MeTrfase_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11061">
        <property type="entry name" value="PTHR11061"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05958">
        <property type="entry name" value="tRNA_U5-meth_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51687">
        <property type="entry name" value="SAM_MT_RNA_M5U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01230">
        <property type="entry name" value="TRMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4 6" id="KW-0808">Transferase</keyword>
    <feature type="active site" evidence="5">
        <location>
            <position position="378"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="378"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="352"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABA03898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="422" mass="45097" checksum="175641CA9BF17BD9" modified="2005-10-11" version="1">MAEPRLQTGGTKRLTIDHVGHRGDGVALTDSGNVHVPYALGGETVEIEPFDGHAGRARLLRIEAASPDRIAPFCSHFGACGGCAIQHWRPEAYRAWKRRIVIDTLAQARIDCDVDELIDAHGAGRRRITLHARSDGGKLRVGFAAPNSHAIVPIDACPVLDPALHGAIDAARELAVALKPANKPLDIQMTATRNGLDVDVRSSGQLPPALIATLSRLAEAHRLARLTRHGELVLMRAAPMIEIGAARLVLPPGSFLQATVAGEEALAALVAVHCGKARRIVDLFCGVGPFALRLAMRAPVAAFDSNAAAVTALQKAADTASRLKPIKAESRDLFRRPLMAQELRDIDAAIFDPPRQGAEAQSKQLAAGRIPVIVAVSCSAATFARDARILVDGGYSLEAVTPVDQFRHSAHVELVAKFVRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SUZ6</accession>
    <name>Q3SUZ6_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphoglycerate/bisphosphoglycerate mutase</fullName>
            <ecNumber evidence="1">5.4.2.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Nwi_0628</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="5.4.2.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313955.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0628"/>
    <dbReference type="EnsemblBacteria" id="ABA03895">
        <property type="protein sequence ID" value="ABA03895"/>
        <property type="gene ID" value="Nwi_0628"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0628"/>
    <dbReference type="eggNOG" id="COG0406">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033323_9_1_5"/>
    <dbReference type="OMA" id="PALNEMN"/>
    <dbReference type="OrthoDB" id="1122642at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="SSF53254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABA03895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="197" mass="21479" checksum="D71D1B29F6F965F9" modified="2005-10-11" version="1">MPTIYYVRHGKTEWNALGRFQGLQDIPLNDLGRAEAVKAGDILRKLLAREGRDPFGLGFVTSPLIRARGTMELMRGALDLSPHDYSVDDRLREVGYGHWEGLTLAEMKAANPDVFAAREADKWSVAPPGGESYASATARLRDWYRSLRTSTVAVAHSGTARALMAVLGLQTAQAAASLRIEQGAVYVLGEGEMIKHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV45</accession>
    <name>Q3SV45_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phospholipid/glycerol acyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.51</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0579</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.51" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313907.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0579"/>
    <dbReference type="EnsemblBacteria" id="ABA03846">
        <property type="protein sequence ID" value="ABA03846"/>
        <property type="gene ID" value="Nwi_0579"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0579"/>
    <dbReference type="eggNOG" id="COG0204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027938_5_1_5"/>
    <dbReference type="OMA" id="MPRPLCY"/>
    <dbReference type="OrthoDB" id="1756450at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003841">
        <property type="term" value="F:1-acylglycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="PlsC" evidence="2">
        <location>
            <begin position="73"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="259" mass="29060" checksum="89124243BC9FC1C8" modified="2005-10-11" version="1">MISIFLRSLVFNILFYVTMAIWMVAALPTFLMPRAVLVRMAKAWGRWNIWLMRVICGTRVKILGTGKIPKGPLIFASKHQSTWETFALFSLIDQPVFILKRELTWIPFFGWYLLKTGMIGIDRKAGLRSLKMLVRMTREQVRLGRQLVIFPEGTRRPIGAPPSYKSGVAMIYAEGGVPCVPVALNSGLCWPRRTFLRYPGTITVEFLDPLPQGLRRDEFMSRLTASIEGATASLVEAGRREQARLSGRVKDAGAGGGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SV78</accession>
    <name>Q3SV78_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">dTDP-4-dehydrorhamnose 3,5-epimerase</fullName>
            <ecNumber evidence="3 7">5.1.3.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Thymidine diphospho-4-keto-rhamnose 3,5-epimerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_0546</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 7">Catalyzes the epimerization of the C3' and C5'positions of dTDP-6-deoxy-D-xylo-4-hexulose, forming dTDP-6-deoxy-L-lyxo-4-hexulose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dTDP-4-dehydro-6-deoxy-alpha-D-glucose = dTDP-4-dehydro-beta-L-rhamnose</text>
            <dbReference type="Rhea" id="RHEA:16969"/>
            <dbReference type="ChEBI" id="CHEBI:57649"/>
            <dbReference type="ChEBI" id="CHEBI:62830"/>
            <dbReference type="EC" id="5.1.3.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Carbohydrate biosynthesis; dTDP-L-rhamnose biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the dTDP-4-dehydrorhamnose 3,5-epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.13" evidence="3 7"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0546"/>
    <dbReference type="EnsemblBacteria" id="ABA03813">
        <property type="protein sequence ID" value="ABA03813"/>
        <property type="gene ID" value="Nwi_0546"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0546"/>
    <dbReference type="eggNOG" id="COG1898">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090940_1_1_5"/>
    <dbReference type="OMA" id="AFNAREF"/>
    <dbReference type="UniPathway" id="UPA00124"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008830">
        <property type="term" value="F:dTDP-4-dehydrorhamnose 3,5-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019305">
        <property type="term" value="P:dTDP-rhamnose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000888">
        <property type="entry name" value="dTDP_sugar_isom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21047">
        <property type="entry name" value="PTHR21047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00908">
        <property type="entry name" value="dTDP_sugar_isom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01221">
        <property type="entry name" value="rmlC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <feature type="site" description="Participates in a stacking interaction with the thymidine ring of dTDP-4-oxo-6-deoxyglucose" evidence="6">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR600888-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600888-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA03813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="198" mass="22480" checksum="18114E26BB6E1EF2" modified="2005-10-11" version="1">MRRRHELTNAKKLKMNVIQTEVPEVVIFEPRLFGDQRGFFLETYQFPRYAEHGVTRPFVQDNLSRSARGVLRGLHLQNPSPQGKLVTVLRGKVLDVAVDVRAGSPNFGRHVAVELSEENRRQLWVPRGFAHGFVVLSETADFFYKCDDLYSPKNELSIRWNDPTIGIDWGVETPSLSAKDADAPLLADVKNFPIHGQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVA9</accession>
    <name>Q3SVA9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0515</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313843.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0515"/>
    <dbReference type="EnsemblBacteria" id="ABA03782">
        <property type="protein sequence ID" value="ABA03782"/>
        <property type="gene ID" value="Nwi_0515"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0515"/>
    <dbReference type="eggNOG" id="COG4372">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060521_1_0_5"/>
    <dbReference type="OMA" id="MVEPIMY"/>
    <dbReference type="OrthoDB" id="1795155at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="120"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="263"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="418" mass="46484" checksum="0589127971FDE251" modified="2005-10-11" version="1">MVEPIMYLAIGFLVSMLCGLMVMPLVHDRAVRLTVKRMEAATPMSMAEIQADRDQLRAEFAMSARRLEMKVDQFRRKVASQSAELGKKADAINRLKVELGEKNERIFSLEGRGKTVGERLQSAEQELAARAETLRSVEQTLAERQSDLGRLRQELSERSVTAESRQAELFVVRAQIERLQSRLNEVENELASTQALLEQQRDETAAVARERDDARKHVENLSQRIVNIDTQLIDKTRDAERLGGRVRALEADLAAQANALTERNAKNSELRRAKTAAERANSELRDEIASLTGGKSPLIDTLRNEMLATERALRAAHDERAKLQRDINVLQRQTESGTVEQMENALLREHINDIAAEVARLAMTIEGPDSPIETILAASATGPAKAPNGSAGVIPVSTLADRIRALQPHAPRSERRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVC0</accession>
    <name>Q3SVC0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Nwi_0504</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313832.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0504"/>
    <dbReference type="EnsemblBacteria" id="ABA03771">
        <property type="protein sequence ID" value="ABA03771"/>
        <property type="gene ID" value="Nwi_0504"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0504"/>
    <dbReference type="eggNOG" id="COG0392">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056539_0_0_5"/>
    <dbReference type="OMA" id="LAMYVLV"/>
    <dbReference type="OrthoDB" id="1179665at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022791">
        <property type="entry name" value="L-PG_synthase/AglD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03706">
        <property type="entry name" value="LPG_synthase_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="138"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="215"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="244"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="289"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="331"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABA03771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="351" mass="38238" checksum="FF2FE102C1130242" modified="2005-10-11" version="1">MLGSIRRAISILRRKRILHKLGVVISVTVIAAAFYVLFHILRDIDVEEVMEAVKRTEWHSIALSGLFVAAGYFTLTFYDLFAVRTIGITHVPYRVTAFAAFTSYSIGHNVGASVFTGGAVRYRIYSAWGMNAIDVAKVCFLAGLTFWLGNAAVLGLGIAYHPEAATSIDQLPPWFNRLIAIAIILGLIAYVIWVGTRPRRVGSGSWTVVLPGGRLTLLQIFIGIVDLGFCALAMYVLLPHQPNVGFVVVAVIFVSATLLGFASHSPGGLGVFDAAMLIGLWQMEKNDLLAAMLLFRLLYYVAPFAISIILLTLREIIVSARMRRVGYARTGSNAESDSKLPYLGEHGDSGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVC1</accession>
    <name>Q3SVC1_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0503</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313831.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0503"/>
    <dbReference type="EnsemblBacteria" id="ABA03770">
        <property type="protein sequence ID" value="ABA03770"/>
        <property type="gene ID" value="Nwi_0503"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0503"/>
    <dbReference type="eggNOG" id="ENOG5030PB8">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2286075_0_0_5"/>
    <dbReference type="OMA" id="WIAVVHR"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="108" mass="11532" checksum="DAE94E436538025F" modified="2005-10-11" version="1">MVYLAGFHWVWLVLSLLLGLAMGWIAVVHRGRGVSRLTARWLAATILALIVLAVMHVVPGRFGYWLDLGLVMLGVYLGGCAVGSWLRDGVVSRQAASGGSTEHSSGTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVJ6</accession>
    <name>Q3SVJ6_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endolytic peptidoglycan transglycosylase RlpA</fullName>
            <ecNumber evidence="3">4.2.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rlpA</name>
        <name evidence="7" type="ordered locus">Nwi_0428</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Lytic transglycosylase with a strong preference for naked glycan strands that lack stem peptides.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the RlpA family.</text>
    </comment>
    <dbReference type="EC" id="4.2.2.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313759.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0428"/>
    <dbReference type="EnsemblBacteria" id="ABA03695">
        <property type="protein sequence ID" value="ABA03695"/>
        <property type="gene ID" value="Nwi_0428"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0428"/>
    <dbReference type="eggNOG" id="COG0797">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042923_6_4_5"/>
    <dbReference type="OMA" id="TAMAMIV"/>
    <dbReference type="OrthoDB" id="2027419at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008932">
        <property type="term" value="F:lytic endotransglycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000270">
        <property type="term" value="P:peptidoglycan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02071">
        <property type="entry name" value="RlpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034718">
        <property type="entry name" value="RlpA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012997">
        <property type="entry name" value="RplA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="SSF50685"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00413">
        <property type="entry name" value="rlpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009992554" description="Endolytic peptidoglycan transglycosylase RlpA" evidence="3">
        <location>
            <begin position="20"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="DPBB_1" evidence="6">
        <location>
            <begin position="80"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="36"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABA03695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="168" mass="17817" checksum="45EE43DB772C2630" modified="2005-10-11" version="1" precursor="true">MLHSRKTTLAFLIRPRTLAAVMTASVLIAGGVTEASAGSRNHHRHHHQGHHAKSHGAGGPAWTNANASLERSARSNRSFSGVASYYGNEAGNRTASGQRFNQNAMTAAHRSLPFGTRLRVTHGSRSVVVTINDRGPFIRGRVLDLSKGAAHAIGLTGRGVGRVVAEVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-05-25" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVK1</accession>
    <name>Q3SVK1_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 10">Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex</fullName>
            <ecNumber evidence="5 10">2.3.1.61</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">2-oxoglutarate dehydrogenase complex component E2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Nwi_0423</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 10">E2 component of the 2-oxoglutarate dehydrogenase (OGDH) complex which catalyzes the second step in the conversion of 2-oxoglutarate to succinyl-CoA and CO(2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>(R)-N(6)-dihydrolipoyl-L-lysyl-[2-oxoglutarate dehydrogenase complex component E2] + succinyl-CoA = (R)-N(6)-(S(8)-succinyldihydrolipoyl)-L-lysyl-[2-oxoglutarate dehydrogenase complex component E2] + CoA</text>
            <dbReference type="Rhea" id="RHEA:15213"/>
            <dbReference type="Rhea" id="RHEA-COMP:10581"/>
            <dbReference type="Rhea" id="RHEA-COMP:10582"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="ChEBI" id="CHEBI:83120"/>
            <dbReference type="EC" id="2.3.1.61"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
        <text evidence="10">Binds 1 lipoyl cofactor covalently.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 6/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.61" evidence="5 10"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313754.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0423"/>
    <dbReference type="EnsemblBacteria" id="ABA03690">
        <property type="protein sequence ID" value="ABA03690"/>
        <property type="gene ID" value="Nwi_0423"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0423"/>
    <dbReference type="eggNOG" id="COG0508">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016733_10_1_5"/>
    <dbReference type="OMA" id="MKVPSPG"/>
    <dbReference type="OrthoDB" id="1626282at2"/>
    <dbReference type="UniPathway" id="UPA00868">
        <property type="reaction ID" value="UER00840"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004149">
        <property type="term" value="F:dihydrolipoyllysine-residue succinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033512">
        <property type="term" value="P:L-lysine catabolic process to acetyl-CoA via saccharopine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036625">
        <property type="entry name" value="E3-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004167">
        <property type="entry name" value="PSBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006255">
        <property type="entry name" value="SucB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02817">
        <property type="entry name" value="E3_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47005">
        <property type="entry name" value="SSF47005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01347">
        <property type="entry name" value="sucB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51826">
        <property type="entry name" value="PSBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9 10" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 10" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="12">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Peripheral subunit-binding (PSBD)" evidence="13">
        <location>
            <begin position="127"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="79"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="169"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00019511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABA03690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="424" mass="45176" checksum="386BB6813A85C0DC" modified="2005-10-11" version="1">MTEIRVPTLGESVTEATIGKWFKKQGDAVSVDEPLVELETDKVTIEVPAPSAGTLAELVARQGETVAVGALLGQITEGGAPAKPAAAKTQGISAESTTGRPDLKSDTTTPINAGPEEPRPRAEADAPLAPSVRKLSAESGIDASTVSGSGKDGRVTKGDMLAAIEKAASAPTPVDQPAAAVQVRAPSPADDASREERVKMTRLRQTIARRLKDVQNTAAILTTFNEVDMSGVMALRAHYKDVFEKKHGVKLGFMGFFTKAIVQALRDIPAVNAEIDGSDLIYKNYYHIGIAVGTDKGLVVPVVRDCDQKSIAEIEKSVADYGRRARDGQLKIEEMQGGTFTITNGGIYGSLMSTPILNAPQAGILGMHKIQERPVAIGGKVEIRPMMYLALSYDHRVIDGKEAVTFLVRVKESLEDPARLVLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVM0</accession>
    <name>Q3SVM0_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cobaltochelatase CobT subunit</fullName>
            <ecNumber evidence="3">6.6.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0404</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.6.1.2" evidence="3"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313735.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0404"/>
    <dbReference type="EnsemblBacteria" id="ABA03671">
        <property type="protein sequence ID" value="ABA03671"/>
        <property type="gene ID" value="Nwi_0404"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0404"/>
    <dbReference type="eggNOG" id="COG4547">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031624_0_0_5"/>
    <dbReference type="OMA" id="WKGGQAR"/>
    <dbReference type="OrthoDB" id="1182388at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051116">
        <property type="term" value="F:cobaltochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006538">
        <property type="entry name" value="CobT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025861">
        <property type="entry name" value="CobT_VWA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06213">
        <property type="entry name" value="CobT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11775">
        <property type="entry name" value="CobT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031715">
        <property type="entry name" value="Cob_chel_CobT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="SSF53300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01651">
        <property type="entry name" value="CobT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="2">
        <location>
            <begin position="413"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="616"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="635" mass="70703" checksum="5D5140CA4E8A3FA2" modified="2005-10-11" version="1">MSTASPSNRFRASPKEAPTEPFKRAVTSCLRAISRRPELEVTFAIERPGLSPGKARLPEPGRKMSRRDAAIVRGHADSIALKLACHDPKVHRKLMPGNPQARGVFDAVEQARVEAIGSRRMTGVARNLTAMLDDHFHRGKYDEITDRADAPLSDALAMMVRERLTGLPPPAAASRLVDLWRPFLEDKIGARLDQLSHFTEDQAKFGDLVHDLLSELDLGDDSRADTEKEENEDDNREGENDQSGTEGSPDSEAAQEMSADQAQDMTDDMPDGAMESAQASVSDTFDDGDLAEEEMPGEATRPNAGGANEPRGPEYHAFAPKFDEVIAAEDLCDHDELERLRSYLDKQLAHLQGIVARLANRLQRRLMAQQNRAWEFDLEEGILDPARLSRVVIDPYQPLSFKHEKEATFRDTVVTLLLDNSGSMRGRPITVAATCADILARTLERCGVKVEILGFTTRAWKGGQSREAWLAAGKPVTPGRLNDLRHIIYKAADAPWRRSRKNLGLMMREGLLKENIDGEALDWAHKRLLGRSEQRKILMMISDGAPVDDSTLSVNPGNYLERHLRWVIEEIETRSPVELIAIGIGHDVTRYYRRAVTIVDAEELGGAMTEQLAELFSETHEPPPGPARRPRKLHS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-11-13" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVQ1</accession>
    <name>GLO2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hydroxyacylglutathione hydrolase</fullName>
            <ecNumber evidence="1">3.1.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glyoxalase II</fullName>
            <shortName evidence="1">Glx II</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gloB</name>
        <name type="ordered locus">Nwi_0373</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an S-(2-hydroxyacyl)glutathione + H2O = a 2-hydroxy carboxylate + glutathione + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21864"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58896"/>
            <dbReference type="ChEBI" id="CHEBI:71261"/>
            <dbReference type="EC" id="3.1.2.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.6" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313704.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SVQ1"/>
    <dbReference type="SMR" id="Q3SVQ1"/>
    <dbReference type="STRING" id="323098.Nwi_0373"/>
    <dbReference type="EnsemblBacteria" id="ABA03640">
        <property type="protein sequence ID" value="ABA03640"/>
        <property type="gene ID" value="Nwi_0373"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0373"/>
    <dbReference type="eggNOG" id="COG0491">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_4_1_5"/>
    <dbReference type="OMA" id="NYIWLLQ"/>
    <dbReference type="OrthoDB" id="733552at2"/>
    <dbReference type="UniPathway" id="UPA00619">
        <property type="reaction ID" value="UER00676"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004416">
        <property type="term" value="F:hydroxyacylglutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019243">
        <property type="term" value="P:methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07723">
        <property type="entry name" value="hydroxyacylglutathione_hydrolase_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01374">
        <property type="entry name" value="Glyoxalase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035680">
        <property type="entry name" value="Clx_II_MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032282">
        <property type="entry name" value="HAGH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017782">
        <property type="entry name" value="Hydroxyacylglutathione_Hdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16123">
        <property type="entry name" value="HAGH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005457">
        <property type="entry name" value="Glx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03413">
        <property type="entry name" value="GSH_gloB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000309667" description="Hydroxyacylglutathione hydrolase">
        <location>
            <begin position="1"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01374"/>
        </source>
    </evidence>
    <sequence length="255" mass="28001" checksum="C50424207AEDF667" modified="2005-10-11" version="1">MTADIRLFTCLTDNFGVLIHDPATGATAAIDAPEAEPVIQALDREGWVLTDILITHHHSDHVGGVAELKRKYACRVVAPHDRSAEIANVDLRVGQGDIIKVGTLLGRVLETPGHTLDHVSYVFDDEKVVFAADTLFSIGCGRVFEGTYPMMWDSLLKLRALPDSFRLYCGHEYTDSNIKFALTIEPNNPVLKARAEEVTRLRAEGRPTVPTLMGEEKKANVFLRADDPAVAAGVHMKGASGVEVFAELRERKNKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVS9</accession>
    <name>Q3SVS9_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">PTS system fructose subfamily IIA component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Nwi_0345</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313677.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0345"/>
    <dbReference type="EnsemblBacteria" id="ABA03612">
        <property type="protein sequence ID" value="ABA03612"/>
        <property type="gene ID" value="Nwi_0345"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0345"/>
    <dbReference type="eggNOG" id="COG2893">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123235_0_1_5"/>
    <dbReference type="OMA" id="RNITCAS"/>
    <dbReference type="OrthoDB" id="2008912at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00006">
        <property type="entry name" value="PTS_IIA_man"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004701">
        <property type="entry name" value="PTS_EIIA_man-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036662">
        <property type="entry name" value="PTS_EIIA_man-typ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033887">
        <property type="entry name" value="PTS_IIA_man"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03610">
        <property type="entry name" value="EIIA-man"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53062">
        <property type="entry name" value="SSF53062"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51096">
        <property type="entry name" value="PTS_EIIA_TYPE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PTS EIIA type-4" evidence="3">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABA03612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="133" mass="14157" checksum="905F6632ABACB471" modified="2005-10-11" version="1">MIGLVLVTHGRLADEFKAALEHVMGPQKQIEAITIGADDDADLCRSDIIEAVNRVDSGDGVAILTDMFGGTPSNLAISCMSRPKVEVLAGINLPMLVKLAKVREERTLPDAIAMAQEAGRKYVTIASRVLAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVZ2</accession>
    <name>Q3SVZ2_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0282</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313615.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0282"/>
    <dbReference type="EnsemblBacteria" id="ABA03549">
        <property type="protein sequence ID" value="ABA03549"/>
        <property type="gene ID" value="Nwi_0282"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0282"/>
    <dbReference type="eggNOG" id="COG5002">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144643_0_0_5"/>
    <dbReference type="OMA" id="WAGYREV"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAS" evidence="1">
        <location>
            <begin position="3"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="2">
        <location>
            <begin position="78"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="141" mass="15309" checksum="FF4C4B87372DF1E6" modified="2005-10-11" version="1">MDASAIAAALLDSSADAIVATDRNGTITLWNPGAVRIFGHSADEATGQSLDLIIPENQRARHWAGYREVMLTGRSRYSDGDVLAVPALHKDGRRISLEFTIVPLTDASGRINGLASVLRDVTARFEEIRQLRRKAAVALRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SVZ8</accession>
    <name>Q3SVZ8_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA gyrase inhibitor YacG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">yacG</name>
        <name evidence="2" type="ordered locus">Nwi_0276</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Inhibits all the catalytic activities of DNA gyrase by preventing its interaction with DNA. Acts by binding directly to the C-terminal domain of GyrB, which probably disrupts DNA binding by the gyrase.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with GyrB.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA gyrase inhibitor YacG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0276"/>
    <dbReference type="EnsemblBacteria" id="ABA03543">
        <property type="protein sequence ID" value="ABA03543"/>
        <property type="gene ID" value="Nwi_0276"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0276"/>
    <dbReference type="eggNOG" id="COG3024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_178280_2_0_5"/>
    <dbReference type="OMA" id="CSNRCRE"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008657">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00649">
        <property type="entry name" value="DNA_gyrase_inhibitor_YacG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005584">
        <property type="entry name" value="DNA_gyrase_inhibitor_YacG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36150">
        <property type="entry name" value="PTHR36150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03884">
        <property type="entry name" value="YacG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="65" mass="7233" checksum="C9E2019BF2E80DF0" modified="2005-10-11" version="1">MPAGWMPVRKPARTRPDKPCPICGKPAAEDSRPFCSERCRDVDLNRWLSGSYAIPASRADDDDAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW21</accession>
    <name>Q3SW21_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Possible ferric siderophore receptor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Nwi_0253</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TonB-dependent receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0253"/>
    <dbReference type="EnsemblBacteria" id="ABA03520">
        <property type="protein sequence ID" value="ABA03520"/>
        <property type="gene ID" value="Nwi_0253"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0253"/>
    <dbReference type="eggNOG" id="COG4774">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008287_2_2_5"/>
    <dbReference type="OMA" id="KQRHRGV"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015891">
        <property type="term" value="P:siderophore transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030149">
        <property type="entry name" value="FcuA/FatA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039423">
        <property type="entry name" value="TonB-dep_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000531">
        <property type="entry name" value="TonB-dep_rcpt_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036942">
        <property type="entry name" value="TonB_rcpt_b-brl_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32552">
        <property type="entry name" value="PTHR32552"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32552:SF82">
        <property type="entry name" value="PTHR32552:SF82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00593">
        <property type="entry name" value="TonB_dep_Rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TonB_dep_Rec" evidence="2">
        <location>
            <begin position="3"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABA03520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="200" mass="21697" checksum="317035D917F116F6" modified="2005-10-11" version="1">MHPYLTKQTEVGVKYDFGTFIATAAVFEFEKQNTIVVPDGSSGPGRLAIDGKQRHRGVEISFFGEPYEGVRLLGGVTYSDARMIATQGGTLDGTFVTGVSRWQGNLGAEWDTPFLDGLTFTGRMIATGSQYASTNNTLRMPGWTRFDVGARYTTTALGNPLTIRGGIENVLGTKYWSQVSGSIFSLGAPRIFKLSATIDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SW63</accession>
    <name>Q3SW63_NITWN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Nwi_0210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313546.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="STRING" id="323098.Nwi_0210"/>
    <dbReference type="EnsemblBacteria" id="ABA03478">
        <property type="protein sequence ID" value="ABA03478"/>
        <property type="gene ID" value="Nwi_0210"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0210"/>
    <dbReference type="eggNOG" id="COG4530">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140210_0_0_5"/>
    <dbReference type="OMA" id="EWGTKRV"/>
    <dbReference type="OrthoDB" id="2048267at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012644">
        <property type="entry name" value="CHP02300_FYDLN_acid"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09538">
        <property type="entry name" value="FYDLN_acid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02300">
        <property type="entry name" value="FYDLN_acid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABA03478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002531"/>
        </source>
    </evidence>
    <sequence length="137" mass="14606" checksum="0598F0436EB025F7" modified="2005-10-11" version="1">MAKSELGTKRICPTTGKKFYDLNKNPVISPYTGEVVPIVVAPVRGRSDAARAAAASGAEATPEPAEAEEMVSLEEADAEENTGKVKKAVVPESEDDIEIDDTLDDDEDDDSTFIADEEEGDEDVTDIIGDVGDDEET</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWE6</accession>
    <name>HSLV_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent protease subunit HslV</fullName>
            <ecNumber evidence="1">3.4.25.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hslV</name>
        <name type="ordered locus">Nwi_0127</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Protease subunit of a proteasome-like degradation complex believed to be a general protein degrading machinery.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP-dependent cleavage of peptide bonds with broad specificity.</text>
            <dbReference type="EC" id="3.4.25.2"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Allosterically activated by HslU binding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">A double ring-shaped homohexamer of HslV is capped on each side by a ring-shaped HslU homohexamer. The assembly of the HslU/HslV complex is dependent on binding of ATP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase T1B family. HslV subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.25.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313464.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWE6"/>
    <dbReference type="SMR" id="Q3SWE6"/>
    <dbReference type="STRING" id="323098.Nwi_0127"/>
    <dbReference type="MEROPS" id="T01.006"/>
    <dbReference type="EnsemblBacteria" id="ABA03395">
        <property type="protein sequence ID" value="ABA03395"/>
        <property type="gene ID" value="Nwi_0127"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0127"/>
    <dbReference type="eggNOG" id="COG5405">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093872_1_0_5"/>
    <dbReference type="OMA" id="IMKGNAR"/>
    <dbReference type="OrthoDB" id="1129370at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009376">
        <property type="term" value="C:HslUV protease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005839">
        <property type="term" value="C:proteasome core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01913">
        <property type="entry name" value="protease_HslV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00248">
        <property type="entry name" value="HslV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022281">
        <property type="entry name" value="ATP-dep_Prtase_HsIV_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="PTHR32194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039093">
        <property type="entry name" value="HslV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03692">
        <property type="entry name" value="ATP_dep_HslV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0021">Allosteric enzyme</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0915">Sodium</keyword>
    <keyword id="KW-0888">Threonine protease</keyword>
    <feature type="chain" id="PRO_1000012641" description="ATP-dependent protease subunit HslV">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00248"/>
        </source>
    </evidence>
    <sequence length="184" mass="19526" checksum="F338CE50027AE247" modified="2005-10-11" version="1">MQASQPETWHGTTILTVRKGGKVVIGGDGQVSIGQTVIKSNAKKVRKLGKGDVIGGFAGATADAFTLFERLESKLEQYPGQLTRAAVELAKDWRTDRYLRRLEAMMLVADKDVSLVLTGTGDVLEPESGVMAIGSGGNYALAAARALIDSDKDAETIVRSALDIAADICVYTNRNLTIEALAAS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-21" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q3SWP9</accession>
    <name>IF2_NITWN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Translation initiation factor IF-2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">infB</name>
        <name type="ordered locus">Nwi_0024</name>
    </gene>
    <organism>
        <name type="scientific">Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)</name>
        <dbReference type="NCBI Taxonomy" id="323098"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Hyphomicrobiales</taxon>
            <taxon>Bradyrhizobiaceae</taxon>
            <taxon>Nitrobacter</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Appl. Environ. Microbiol." volume="72" first="2050" last="2063">
            <title>Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255.</title>
            <authorList>
                <person name="Starkenburg S.R."/>
                <person name="Chain P.S."/>
                <person name="Sayavedra-Soto L.A."/>
                <person name="Hauser L."/>
                <person name="Land M.L."/>
                <person name="Larimer F.W."/>
                <person name="Malfatti S.A."/>
                <person name="Klotz M.G."/>
                <person name="Bottomley P.J."/>
                <person name="Arp D.J."/>
                <person name="Hickey W.J."/>
            </authorList>
            <dbReference type="PubMed" id="16517654"/>
            <dbReference type="DOI" id="10.1128/AEM.72.3.2050-2063.2006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000115">
        <property type="protein sequence ID" value="ABA03292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011313363.1">
        <property type="nucleotide sequence ID" value="NC_007406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3SWP9"/>
    <dbReference type="SMR" id="Q3SWP9"/>
    <dbReference type="STRING" id="323098.Nwi_0024"/>
    <dbReference type="EnsemblBacteria" id="ABA03292">
        <property type="protein sequence ID" value="ABA03292"/>
        <property type="gene ID" value="Nwi_0024"/>
    </dbReference>
    <dbReference type="KEGG" id="nwi:Nwi_0024"/>
    <dbReference type="eggNOG" id="COG0532">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006301_10_0_5"/>
    <dbReference type="OMA" id="NRDNRTG"/>
    <dbReference type="OrthoDB" id="347113at2"/>
    <dbReference type="Proteomes" id="UP000002531">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03702">
        <property type="entry name" value="IF2_mtIF2_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00100_B">
        <property type="entry name" value="IF_2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013575">
        <property type="entry name" value="IF2_assoc_dom_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044145">
        <property type="entry name" value="IF2_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006847">
        <property type="entry name" value="IF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000178">
        <property type="entry name" value="TF_IF2_bacterial-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="PTHR43381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08364">
        <property type="entry name" value="IF2_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04760">
        <property type="entry name" value="IF2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="SSF52156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00487">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01176">
        <property type="entry name" value="IF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000228217" description="Translation initiation factor IF-2">
        <location>
            <begin position="1"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="domain" description="tr-type G">
        <location>
            <begin position="367"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="G1" evidence="1">
        <location>
            <begin position="376"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="G2" evidence="1">
        <location>
            <begin position="401"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="G3" evidence="1">
        <location>
            <begin position="424"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="G4" evidence="1">
        <location>
            <begin position="478"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="G5" evidence="1">
        <location>
            <begin position="514"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="8"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="41"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="97"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="182"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="376"/>
            <end position="383"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="424"/>
            <end position="428"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="478"/>
            <end position="481"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="871" mass="93665" checksum="A6B619F436A55F2B" modified="2005-10-11" version="1">MVDTKNPGDKTLSVSPTKTLTLKPRVEQGTVRQSFSHGRTKQVVVEKRGKRRIGGDSPGPEPAGTSEPASARTPAAKAPPARAATPAAPRAGSGVVLRKLTEDERSARASALAEAKVRAEEERRIAEAEAARRNSKEGIEQAEREAAEARRKAEEERHRQEEEAKRKAEIEAKRRFGQTESKPAPAKTTTTTTRAAPPARPAAVAADGSDEDEAPRLVRRPGGPARPVIAPKPAAKPAPAKQRGRLTLVTALSADDVRERSIASFRRRTQRLKGHASNEPKEKLVREVVVPEAITIQELANRMAERAVDVIRMLMKQGAMHKITDVIDADTAQLIAEELGHTVKRVAASDVEEGLFDVVDNSTDIEPRSPVVTVMGHVDHGKTSLLDALRHANVVSGEAGGITQHIGAYQVTSPESGKKITFIDTPGHAAFTAMRARGAKVTDLVILVVAADDGVMPQTIEAINHAKAAKVPMIIAINKIDKPEAKPERVRTELLQHEVQVESMGGQVVDVEVSAKNKTNLDKLLEMISLQADLLDLKTNASRPAEGTVIEAKLDRGRGPVATVLVQRGTLRVGDIIVAGAEMGRVRALISDQGETVNEAGPSVPVEVLGFNGPPEAGDRLAVVENEARARQVTSYRAHQKRENAAASTSGMRGSLEQMMSQLKTVGRKDFPLIIKADVQGSLEAILGSLEKLGTDEVAARILHAGVGGISESDVTLAEGFNAAIIGFSVRANKEAAAAAKRNGIEIRYYNIIYDLVDDVKKAMSGLLAPTLRETMLGNAEILEVFNISKVGKVAGCRVTDGSVERGANVRLIRDNVVVHEGKLSTLKRFKDEVKEVQSGQECGMAFESYGDMRVGDVIECYRVETIQRSL</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>